LOCUS       DAZULO010000001        89654 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000001 DAZULO010000000
VERSION     DAZULO010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 89654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.001"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(553..2166)
                     /locus_tag="ACG8OA_000001"
     CDS             complement(553..2166)
                     /locus_tag="ACG8OA_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4974857.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2307..3215)
                     /locus_tag="ACG8OA_000002"
     CDS             complement(2307..3215)
                     /locus_tag="ACG8OA_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4974858.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3398..4522
                     /locus_tag="ACG8OA_000003"
     CDS             3398..4522
                     /locus_tag="ACG8OA_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4974859.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4749..5666
                     /locus_tag="ACG8OA_000004"
     CDS             4749..5666
                     /locus_tag="ACG8OA_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4974860.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5659..6492
                     /locus_tag="ACG8OA_000005"
     CDS             5659..6492
                     /locus_tag="ACG8OA_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4974861.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6575..7693
                     /locus_tag="ACG8OA_000006"
     CDS             6575..7693
                     /locus_tag="ACG8OA_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4974862.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7714..9585
                     /locus_tag="ACG8OA_000007"
     CDS             7714..9585
                     /locus_tag="ACG8OA_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFS4974863.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            9579..10802
                     /locus_tag="ACG8OA_000008"
     CDS             9579..10802
                     /locus_tag="ACG8OA_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4974864.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10888..12822)
                     /locus_tag="ACG8OA_000009"
     CDS             complement(10888..12822)
                     /locus_tag="ACG8OA_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4974865.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13048..13704
                     /locus_tag="ACG8OA_000010"
     CDS             13048..13704
                     /locus_tag="ACG8OA_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS4974866.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13778..14131)
                     /locus_tag="ACG8OA_000011"
     CDS             complement(13778..14131)
                     /locus_tag="ACG8OA_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS4974867.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14323..15252
                     /locus_tag="ACG8OA_000012"
     CDS             14323..15252
                     /locus_tag="ACG8OA_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFS4974868.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15263..16099
                     /locus_tag="ACG8OA_000013"
     CDS             15263..16099
                     /locus_tag="ACG8OA_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFS4974869.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16347..17123
                     /locus_tag="ACG8OA_000014"
     CDS             16347..17123
                     /locus_tag="ACG8OA_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS4974870.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17299..18042
                     /locus_tag="ACG8OA_000015"
     CDS             17299..18042
                     /locus_tag="ACG8OA_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4974871.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18035..19615
                     /locus_tag="ACG8OA_000016"
     CDS             18035..19615
                     /locus_tag="ACG8OA_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFS4974872.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19866..20345)
                     /locus_tag="ACG8OA_000017"
     CDS             complement(19866..20345)
                     /locus_tag="ACG8OA_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS4974873.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20361..21113)
                     /locus_tag="ACG8OA_000018"
     CDS             complement(20361..21113)
                     /locus_tag="ACG8OA_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS4974874.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21230..21300
                     /locus_tag="ACG8OA_000019"
     tRNA            21230..21300
                     /locus_tag="ACG8OA_000019"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21262..21264,aa:Gly,seq:ccc)
     gene            21661..22386
                     /locus_tag="ACG8OA_000020"
     CDS             21661..22386
                     /locus_tag="ACG8OA_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFS4974875.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22641..22907
                     /locus_tag="ACG8OA_000021"
     CDS             22641..22907
                     /locus_tag="ACG8OA_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFS4974876.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22904..23335
                     /gene="ruvX"
                     /locus_tag="ACG8OA_000022"
     CDS             22904..23335
                     /gene="ruvX"
                     /locus_tag="ACG8OA_000022"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFS4974877.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23360..23665
                     /locus_tag="ACG8OA_000023"
     CDS             23360..23665
                     /locus_tag="ACG8OA_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFS4974878.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(23746..24891)
                     /locus_tag="ACG8OA_000024"
     CDS             complement(23746..24891)
                     /locus_tag="ACG8OA_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4974879.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(24952..25599)
                     /locus_tag="ACG8OA_000025"
     CDS             complement(24952..25599)
                     /locus_tag="ACG8OA_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4974880.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            25793..26086
                     /locus_tag="ACG8OA_000026"
     CDS             25793..26086
                     /locus_tag="ACG8OA_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4974881.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            26130..26441
                     /locus_tag="ACG8OA_000027"
     CDS             26130..26441
                     /locus_tag="ACG8OA_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4974882.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            26438..27062
                     /locus_tag="ACG8OA_000028"
                     /pseudo
     CDS             26438..27062
                     /locus_tag="ACG8OA_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            27203..27361
                     /locus_tag="ACG8OA_000029"
     CDS             27203..27361
                     /locus_tag="ACG8OA_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4974883.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            27362..27724
                     /locus_tag="ACG8OA_000030"
     CDS             27362..27724
                     /locus_tag="ACG8OA_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4974884.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            27761..28609
                     /locus_tag="ACG8OA_000031"
     CDS             27761..28609
                     /locus_tag="ACG8OA_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFS4974885.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            28599..30074
                     /locus_tag="ACG8OA_000032"
     CDS             28599..30074
                     /locus_tag="ACG8OA_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFS4974886.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            30340..30747
                     /locus_tag="ACG8OA_000033"
     CDS             30340..30747
                     /locus_tag="ACG8OA_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFS4974887.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            30750..30965
                     /locus_tag="ACG8OA_000034"
     CDS             30750..30965
                     /locus_tag="ACG8OA_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4974888.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            30969..31349
                     /locus_tag="ACG8OA_000035"
     CDS             30969..31349
                     /locus_tag="ACG8OA_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS4974889.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            31483..31638
                     /locus_tag="ACG8OA_000036"
     CDS             31483..31638
                     /locus_tag="ACG8OA_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFS4974890.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            31707..32180
                     /locus_tag="ACG8OA_000037"
     CDS             31707..32180
                     /locus_tag="ACG8OA_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFS4974891.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            32177..33871
                     /locus_tag="ACG8OA_000038"
     CDS             32177..33871
                     /locus_tag="ACG8OA_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS4974892.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            33837..34022
                     /locus_tag="ACG8OA_000039"
     CDS             33837..34022
                     /locus_tag="ACG8OA_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4974893.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            34026..35201
                     /locus_tag="ACG8OA_000040"
     CDS             34026..35201
                     /locus_tag="ACG8OA_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4974894.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            35194..36717
                     /locus_tag="ACG8OA_000041"
     CDS             35194..36717
                     /locus_tag="ACG8OA_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS4974895.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            36773..37057
                     /locus_tag="ACG8OA_000042"
     CDS             36773..37057
                     /locus_tag="ACG8OA_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFS4974896.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            37044..37379
                     /locus_tag="ACG8OA_000043"
     CDS             37044..37379
                     /locus_tag="ACG8OA_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS4974897.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            37674..38300
                     /locus_tag="ACG8OA_000044"
     CDS             37674..38300
                     /locus_tag="ACG8OA_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4974898.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            39042..39879
                     /locus_tag="ACG8OA_000045"
                     /pseudo
     CDS             39042..39879
                     /locus_tag="ACG8OA_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            39944..40378
                     /locus_tag="ACG8OA_000046"
     CDS             39944..40378
                     /locus_tag="ACG8OA_000046"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4974899.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(40446..41768)
                     /locus_tag="ACG8OA_000047"
     CDS             complement(40446..41768)
                     /locus_tag="ACG8OA_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFS4974900.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(41922..43562)
                     /locus_tag="ACG8OA_000048"
     CDS             complement(41922..43562)
                     /locus_tag="ACG8OA_000048"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFS4974901.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            43892..44362
                     /locus_tag="ACG8OA_000049"
     CDS             43892..44362
                     /locus_tag="ACG8OA_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFS4974902.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            44991..46094
                     /locus_tag="ACG8OA_000050"
     CDS             44991..46094
                     /locus_tag="ACG8OA_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFS4974903.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            46378..47058
                     /locus_tag="ACG8OA_000051"
     CDS             46378..47058
                     /locus_tag="ACG8OA_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS4974904.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            47148..48182
                     /locus_tag="ACG8OA_000052"
     CDS             47148..48182
                     /locus_tag="ACG8OA_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS4974905.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            48196..48531
                     /locus_tag="ACG8OA_000053"
     CDS             48196..48531
                     /locus_tag="ACG8OA_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4974906.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            48515..50995
                     /locus_tag="ACG8OA_000054"
     CDS             48515..50995
                     /locus_tag="ACG8OA_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4974907.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            51051..52610
                     /locus_tag="ACG8OA_000055"
     CDS             51051..52610
                     /locus_tag="ACG8OA_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4974908.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            52607..53971
                     /locus_tag="ACG8OA_000056"
     CDS             52607..53971
                     /locus_tag="ACG8OA_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFS4974909.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            54142..55212
                     /locus_tag="ACG8OA_000057"
     CDS             54142..55212
                     /locus_tag="ACG8OA_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4974910.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            55227..55895
                     /locus_tag="ACG8OA_000058"
     CDS             55227..55895
                     /locus_tag="ACG8OA_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4974911.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            55915..56493
                     /locus_tag="ACG8OA_000059"
     CDS             55915..56493
                     /locus_tag="ACG8OA_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4974912.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            56896..57807
                     /locus_tag="ACG8OA_000060"
     CDS             56896..57807
                     /locus_tag="ACG8OA_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS4974913.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(58063..58980)
                     /gene="rnhC"
                     /locus_tag="ACG8OA_000061"
     CDS             complement(58063..58980)
                     /gene="rnhC"
                     /locus_tag="ACG8OA_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFS4974914.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            59182..59619
                     /gene="zapA"
                     /locus_tag="ACG8OA_000062"
     CDS             59182..59619
                     /gene="zapA"
                     /locus_tag="ACG8OA_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFS4974915.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            59677..60224
                     /locus_tag="ACG8OA_000063"
                     /pseudo
     CDS             59677..60224
                     /locus_tag="ACG8OA_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            60339..62699
                     /locus_tag="ACG8OA_000064"
     CDS             60339..62699
                     /locus_tag="ACG8OA_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFS4974916.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            62786..63100
                     /gene="trxA"
                     /locus_tag="ACG8OA_000065"
     CDS             62786..63100
                     /gene="trxA"
                     /locus_tag="ACG8OA_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFS4974917.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            63282..65060
                     /gene="uvrC"
                     /locus_tag="ACG8OA_000066"
     CDS             63282..65060
                     /gene="uvrC"
                     /locus_tag="ACG8OA_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFS4974918.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            65205..66104
                     /locus_tag="ACG8OA_000067"
     CDS             65205..66104
                     /locus_tag="ACG8OA_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4974919.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            66097..67359
                     /locus_tag="ACG8OA_000068"
     CDS             66097..67359
                     /locus_tag="ACG8OA_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4974920.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            67522..69420
                     /locus_tag="ACG8OA_000069"
     CDS             67522..69420
                     /locus_tag="ACG8OA_000069"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFS4974921.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(69719..70438)
                     /locus_tag="ACG8OA_000070"
     CDS             complement(69719..70438)
                     /locus_tag="ACG8OA_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFS4974922.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    70628..70675
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            70775..71107
                     /locus_tag="ACG8OA_000071"
     CDS             70775..71107
                     /locus_tag="ACG8OA_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4974923.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            71094..73097
                     /locus_tag="ACG8OA_000072"
     CDS             71094..73097
                     /locus_tag="ACG8OA_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFS4974924.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            73116..73586
                     /locus_tag="ACG8OA_000073"
     CDS             73116..73586
                     /locus_tag="ACG8OA_000073"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFS4974925.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            73607..74191
                     /locus_tag="ACG8OA_000074"
     CDS             73607..74191
                     /locus_tag="ACG8OA_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFS4974926.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            74206..75192
                     /locus_tag="ACG8OA_000075"
     CDS             74206..75192
                     /locus_tag="ACG8OA_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFS4974927.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            75182..75493
                     /locus_tag="ACG8OA_000076"
     CDS             75182..75493
                     /locus_tag="ACG8OA_000076"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFS4974928.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            75515..77296
                     /locus_tag="ACG8OA_000077"
     CDS             75515..77296
                     /locus_tag="ACG8OA_000077"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFS4974929.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            77289..78665
                     /locus_tag="ACG8OA_000078"
     CDS             77289..78665
                     /locus_tag="ACG8OA_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFS4974930.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            78672..79307
                     /locus_tag="ACG8OA_000079"
     CDS             78672..79307
                     /locus_tag="ACG8OA_000079"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFS4974931.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            79337..80692
                     /locus_tag="ACG8OA_000080"
     CDS             79337..80692
                     /locus_tag="ACG8OA_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS4974932.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(80884..82278)
                     /locus_tag="ACG8OA_000081"
     CDS             complement(80884..82278)
                     /locus_tag="ACG8OA_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS4974933.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            82370..83341
                     /locus_tag="ACG8OA_000082"
     CDS             82370..83341
                     /locus_tag="ACG8OA_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFS4974934.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            83338..84120
                     /locus_tag="ACG8OA_000083"
     CDS             83338..84120
                     /locus_tag="ACG8OA_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFS4974935.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(84289..84660)
                     /locus_tag="ACG8OA_000084"
     CDS             complement(84289..84660)
                     /locus_tag="ACG8OA_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4974936.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            84794..85237
                     /locus_tag="ACG8OA_000085"
     CDS             84794..85237
                     /locus_tag="ACG8OA_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS4974937.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(85280..86089)
                     /gene="yidA"
                     /locus_tag="ACG8OA_000086"
     CDS             complement(85280..86089)
                     /gene="yidA"
                     /locus_tag="ACG8OA_000086"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFS4974938.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(86108..87475)
                     /locus_tag="ACG8OA_000087"
     CDS             complement(86108..87475)
                     /locus_tag="ACG8OA_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4974939.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(87459..88289)
                     /locus_tag="ACG8OA_000088"
     CDS             complement(87459..88289)
                     /locus_tag="ACG8OA_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFS4974940.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            88452..88889
                     /locus_tag="ACG8OA_000089"
     CDS             88452..88889
                     /locus_tag="ACG8OA_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFS4974941.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            88965..89585
                     /gene="rpoE"
                     /locus_tag="ACG8OA_000090"
     CDS             88965..89585
                     /gene="rpoE"
                     /locus_tag="ACG8OA_000090"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFS4974942.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        29552 a        15170 c        18472 g        26460 t
ORIGIN      
        1 gttaaataaa taggaacaaa aaaattaaat tatttaatac acacattagg gatcattcat
       61 tgaaggatta gaagacaacg aggatggcga aaaatatgat tacaatagaa aagtcgttct
      121 aaaccaatca gttcctagta tcacacaacg caagccagtc gatttggtga gattagagga
      181 gtgaaagcaa actttattat ataagaaaaa atattcgaaa gggatgctag tttaggtact
      241 agtgctgata gattaatatg atgcctcgta atattaggcg tagttactct agagacatca
      301 ctatggacta tgatttgtct cagagtcaca acatgttttt ttatgaaaag aaacagctaa
      361 aataattaaa ttgcgcaatg tttaaacttt cttaaactat aacctaataa atgtgaagaa
      421 ataatgcaca atttcttcac atttatcttc tattctatat acataccaac caacaaccct
      481 atatactttt tcatttttac tcctcaagta aaaataactc ctagaccgcg gctacccacc
      541 gcggtcgttt ttttattctt caatcaaaat cgctttgata cccaaacctg ccaagattcg
      601 ggcagaagta taagtattca atcctcttct gaaagtaatg taaacttttt tatcttttgg
      661 gatttcattg attcgatctc gtaactcatt cattggaata ttttttgttg ccgagatact
      721 gccagaaggt gctttattag gttcacgtat atctaaaaag aacgcttctt tcaaatcgtt
      781 ttcgggtata tcacttgctt tgatcgtttc tacgatattc gtcatttgat tgattgcgac
      841 ataaccagct atattcaacg ggtcacgggt agtagaatac ggtggagagt aaggcaactc
      901 caaatcaggc aaatcaaaga ctgtcaagtt tccggtgatt gctacagaaa gttctcccat
      961 ccgtttatcg acaccttttt ctccaaatgc ttgtccacct aaaatacgac cgctatctgc
     1021 atcaaaaatc agctttagat ttaatcttgt agcttctgga tagaaatagg catggtcaaa
     1081 gggagtgata aaaacagttt tataatttgt tatgcctgat gcttttaatt catgttctgt
     1141 cattcccgca taacttgctg tatgatcaaa gatttttgcg acacctgcac caatatatcc
     1201 gcgatagcgc ataggcgtac catttaaaat atctgccagc atatgtcctt gacgattggc
     1261 cgcactggaa agcatacttt ggatcggctg accagtcact acactagtcg tttcgataat
     1321 atcgccaatc gcatagatgt ccggcagatt ggtttgtaac tggtcatcaa caatgatctg
     1381 acctgtatct gataattgta tgccggttgc tttcaccact tcattcttcg gagaaacacc
     1441 aacagcaaaa attagcatat cagcagaaag gacggagcca tctgataata ctatttctcg
     1501 acctttatca cggatctcag taactcttgt ttctaaatga acggctaatc cttctttcag
     1561 caatttgtca taaactaaat tagcaatttc tggatcataa gggaaagcga cttgtggcaa
     1621 ttgatcgatt aaagtcacgt ctaagccgcg atgcttgaaa ttctcagcta attccagacc
     1681 cataacacct gcaccaagaa tgacgacatg ttgtggattt ttttcttcaa ggaacgattt
     1741 gattcgatcc gcatccgtca cactacggag gacaaagccg ttttctgctt caaaaactcc
     1801 gggaatatcc ggataatctg gtctagcacc agaagagatg atcaacttat cgtaatctgt
     1861 ttttatttct tcatttgtgg ataggtcttt aactgttaat cgcttagtag aaggatcgat
     1921 tgctgttact tcgtgtttag taaagacatc gatgttgttt tttgttttca atatttctgg
     1981 tgtacgttcg atcagcgagt cacggtcagt gatcacacct cctaaataat aaggcaaagc
     2041 acaacttgca taagaaatat tttctccgcg ttcatagata atgatttcgt gcgcttcatt
     2101 caatcgacgg aaacgagtgg caaatgaagg accgccagct acaccgccaa tgacaacaac
     2161 tttcataaaa acatctcctt ttctacgttg atcgtactat gttatggtaa aaaacagcaa
     2221 ataattgtgc cggaaaatta ttgtttattt tgaatagaca gaaaatatag agaaataatt
     2281 tgtggataaa acctaaaaat ttttttctat tttaaggata gataatactt tgaaggggat
     2341 aaccctgtat attttttaaa agctgaagaa aaatgatgga tcgaatgaaa tcctaatata
     2401 tctgcaattt ctgtataggt taattcattt tctagcacga gacgttttgc tttttccatt
     2461 tttagttctt taaaatagct aataacactt tttccattca cttttctaaa atgttttttt
     2521 aagctggctg gactgataaa aaaaatttgg gctaattgtt caagcaaaaa attttctgaa
     2581 agatgttctt ctaaatatcg tatgattgga tcaactaatt catctgaata aagaatatgc
     2641 ttttttttag gaatgacttt ctcatttgaa ggtattgtca ataaagcaag ttcctgttga
     2701 taatgccttg gtaaggaaga caattgaatt ttttgcagac aataagtaac taataaggaa
     2761 agcaaatgaa tgctcatctg ttggcatagt atgaaaaaag aattgatttc ctgatcatag
     2821 gaaatagctg gcttactatg ttttagataa tcaattttga actgttcatt tttcagaaaa
     2881 gtaatgatag atgttatttc ttgatctata gaaaaagatt gtcccgtaat atgtgctaat
     2941 aattgctcat caaaataaaa ctggataatg ataaaagttt gtgtttcctt ttgagtagaa
     3001 aagatttgga cttcttgctg tttatcaatg aataaaaggg aaggattatc tagttctata
     3061 gatttatgtg caaattcagc atataaatta gcgctttcat ttattagtag tcgaaaatag
     3121 ggagcagcag ataaatgaag atgggtatct atggaatagg ttagtgtttt tttgacaaga
     3181 ttggaggatt ggttgtttgt taacaaaaga accactttcc tttctctttt tatataaaaa
     3241 gctttctatt tataattaat gactattata acaaaaatga aaagccaaaa cgacaagtaa
     3301 aatatttttt tatataaata tttgtgttac taattccgtt aatatacata agtgaaagcg
     3361 ttttatataa gaacttgaaa agaaagaagg gatagacatg aaaaaaagat acaaattggt
     3421 gatagctttg ctaaacatta ccctattatt cagcttaagt gcatgtggaa aaactaacga
     3481 agaagtaaca gcaaacatcg cagatccaat gataaaaaaa gccactacat tagaagtata
     3541 tgtcgcaaac ggcaaactga caaactatgt tgaaaatgct gcaaaaatct ataaccgcaa
     3601 aactggagca aatattgact taaaaattgt gaatgtagct tcgggaacag caacagtaca
     3661 aatgatcacc ccaaaattgg taacacacga agatatgccg gatattatct ccatggcaga
     3721 cacttcagca gcaggtgtgc tggaaaaatt tcaagatgct ttttatagtg ctgatgaata
     3781 tggattttat gacaaatatg gcgatcagtt ttatgatcaa aaattgaata ttttgcgtca
     3841 acaaacgagt aatgaaacga ttattccttg ggctagtgat tttaccccag cactttgtta
     3901 ttatcaacca aaaaaatttg ctgaaatagg tgtagatttt gaagaaatca gttcttggga
     3961 agaatatatc caggtggcaa aagcattaaa agaaaaaact ggtagttttg gaattgcctt
     4021 gccagaatct ggggaccaag aattgtttat caacatgcta gcccagcaga atcaatcttt
     4081 gcttgatgaa gaaggaaata tccgtctgaa tactaaagaa gcgaaacatg ctgcaaagtt
     4141 aatcaaagaa atgatcgact cagatattgt acatttctac ggtgcacaag atgctgagaa
     4201 agcatttcaa gaatctgcaa tgtttgttgc tggcggctgg tatgctacga atatgtcttt
     4261 gaattttcct gatgcatctg acaaatggcg catggctcca ctgattcctt tttcaaaaga
     4321 aaatccagga aaaggtgcag tatcaggagg atcaagtttt tatgtcccta aagatgccaa
     4381 aaatccacaa gtggcacaac aatttcttac atttatgctg acagatgaag aatgcttagc
     4441 caacgcactg gagctgggcg tggctacatc taactcattg gcctatcaaa cagaagcagc
     4501 tgagaaaaag tttgactact aaggaggaca aaaatactat gaactattga ataaaatcaa
     4561 tgaaaataca tcaaatactg ttttaccagc cacttattca gatgctacgt cttatttgac
     4621 taccgctgca tataactatt ggcaaacagg tgattttgaa aattcatatc taaaagaagc
     4681 agaaaatttt gctcataagt acggcgtaaa attaactggt gattaaaaaa tattaggagg
     4741 acttaacgat gatgcaatct tcgttaccaa gcaagacaat aaagaacagc aagaaaaaag
     4801 caacagatac gtctcataaa aaaagtgctt atggattggt cacacctttt gttattttct
     4861 ttattgtgac gattttgatt cccatcatta tctcgtttgc ttttagtttt atgaaaattg
     4921 gctactcatt tgaatttgtt ggatttgaca attatctagc tatcttccga gatccattgt
     4981 ttattaaatc ttacacgaat gttttgatta tcatggcagg atcaattcca ataacgatgc
     5041 tattagctat ttactttgct gttttattga attcagcaca gttaaaagga aaaggatttt
     5101 ttagaactgt ctattatatt ccaaccatta catcaacggt ggctgtagct tccgtattca
     5161 tgactttttt caatccgact gggttattca atggattttt gaatgcgcta ggaattaacg
     5221 gtgtacagtg gttaacaaat cctttttgga tacgaatatc aatgatcatc acgatgattt
     5281 ggcttagtgt aggatataac actattttat ttttagctgg tcttcaaggg atttctaaag
     5341 agatatacga aagtgctgaa atagatggag catcgaaatt tcggcaattc tttttattaa
     5401 cgatccccat gctaaagcca atcattttaa tggccacagt tttagccact atcaatggct
     5461 tgggtaattt caatatccca aatatattct ttgggacttc caatggacca gaaaattcag
     5521 ctttggtcgt aggagtcaat ttatataaaa caagttttga aatggttgat tttggaaaag
     5581 cctctgctat tgcctggacg atggtgtttg tatctgttat attatcggct attcaattta
     5641 aattcggagg tgataaaaat gactaaacaa aagatacaaa gcagaatttt tacgtacgtc
     5701 attattgtgt ttggtgcctt aatatttttg atgccatatt tatatatgat tgtagcttca
     5761 acgcaaagta atcaagaaat tttaagtggc ggaatcaatt tcaagtttgg ggatatgcta
     5821 ttaaaaaact ggcaagccat catggcgaga tataattatc ccttgattat ttggaattcg
     5881 attgttattg ctgtaatcag tacagtttta gctacagcca gttctacaat ggcgggttat
     5941 gcattagcaa agtatcgttt tatggggaat aaactgttgt tcaatacagt tatgctatct
     6001 aggatggtgc ctttttttac cacattgatt cctttatttt acattatgag tcgtttagga
     6061 ttatcgaata cttatgcagg tgtgatcatc ccttcgattg ccagtacaac ttcagtgttt
     6121 atgatgcgac aatatagcag tcaatttcct acagcactga tggaggctgc cagaatcgat
     6181 ggtgccaatg agtgggtgat ttttacgaaa atcgctgtac cgattttatc acctacgatt
     6241 attactagtg gtttacttat ctttatggga agttggaatc aatatctatt tccattagtc
     6301 atgctttcag ataaagataa atatacggtt cccttaatta tcaaaaatat gacggtttct
     6361 agtacaggtg agccaattaa ttatggcgca attatgctaa ttttagcaac ttcagtgatc
     6421 ccaatggtct tgatttatgt gtgggcgcag tctaaattta aggagaatga cattggagct
     6481 gcaaataagt aaaaaaaata acgaatgtta accatctagt tcgttctagg agcaactcca
     6541 cattcataaa aattaaaaga atggcgggat atccatgaat gaacagacga aggaatggtc
     6601 aagaaaaatt ttgaatgtag cttctgaaaa gatagatgcg gaattagaca gaatggggaa
     6661 taaaattcca tatatttctt acaacggaaa atatacagac gtaataaagg acagaggaat
     6721 cgactggtgg accaatggtt tttggagtgg tattttatgg cagctctttc acaagacaaa
     6781 acgagaaaag ttcaaagagg cagcaataca acaagaacaa ttattagaat ctgccctgta
     6841 ccatttttca gacgttcatc atgatgtagg ttttttattc ttaccatcgg ctattgcaca
     6901 ttatcaacag tctcaaaaca aacatagtcg acaactaggg ttacatgcag ctacggtact
     6961 tgctggacgc tataatattt cagggaacta tttagtagca tggaatgacc ataaacctgg
     7021 ttggatgatt attgatagta tgatgaattt accattactg tattgggctt cgagggaaac
     7081 tggtgatccg aggttctcta aaattgctcg gcagcatgca gatacaacag ctaagtatct
     7141 agtgaggaaa gatggatcaa ccgggcatat cgcagaattt gatgcagaat cagggaaata
     7201 tattcggtta cttggcgggc aaggatacaa tgagaattct tgttggagca gaggaaatgc
     7261 atgggcttta tatggattcg cgttaagtta tcgttacaca aaaaatccgt actatctagc
     7321 tatagcagaa aagatagcag taaattttat tcagaaaatc gaaaaaactg gttatattcc
     7381 tctcattgat tttgctgtaa aagattcgaa tattgatacc agtgctggag tatgcgctgc
     7441 atgcggtctt ctggaattag caggatatgt ttcctctgta cgatcaaaaa tgtatcatga
     7501 agctgctatc aatatactcc aaaagacaga aaagcactat gcaaattgga atatggatga
     7561 agatgggatc attggaggcg gagcagaagc ataccataga gaactgactt ataatgtgcc
     7621 actgatctat tctgattatt tttttattga aggcttactt cgattagaac agtcggaatt
     7681 agctatttgg tagtaaaaag aggagaaaat acaatgaaac atccagtacc actattaagg
     7741 accagacagg attactttga cttatttaaa aagataaatc aaccactcca gccgttttat
     7801 cgagcaagtg gcgcacaaat tgattatata catcaagggg taggatatgg aaataaaatt
     7861 gcaggaatgg aaggattctc tcgggtttta tggggagcgg ggcccgcaat tgaccaatta
     7921 gatgaacgat ggttgaaatt aattctatca ggaataaaag ctggaacaga tccttcccat
     7981 cccgattatt ggggagaaat acatgaaaga gaccagcgta tggtagaaat gcccgcaatg
     8041 ttattagcgt tatatcatca tgaacaatta ttatggaaaa aattagatac aggagaaaaa
     8101 agccagatta ttcattggtt ctcgcaaatt tttgaatatg aatgtgcaga tggtaattgg
     8161 caatttttta agataattgt cggaacaatt atccaaaaat ttggttatcc agttaaagaa
     8221 tcagcactga aagaagcatt tgataatata gaaaattgtt atcttcaaaa tggctggtat
     8281 cgagacagtt caagaggtcg ccaagattat tataatcctt ttgcatttca ttattatggt
     8341 ttgatttata gcgtactaga accagaaaag cccattagcc agatatataa gaaccgagct
     8401 attgattttt ctagagacta tattcatttt tttgcggaag atggtgcaaa tgtgcctttt
     8461 ggtcgaagca tgatttatcg ttttgcagtt tcaagctttt ggtcagctat gttgtgggcc
     8521 gatcttttgc cttttaaatt aggtgaaatc aaggggttag tcaatcggaa tataagatgg
     8581 tggatgcaaa aagaaatatt tgatgaaaaa ggtatcctga atattggtta tacctaccct
     8641 caattgacat tgactgaacc ctataatagt acgttatctc cattatggct gaataagatt
     8701 tttttattgt tggcgctacc tgaagaccat gagtattgga cggctaaaga aatgcctatg
     8761 cctattcgtg aaggaactaa acttctttca gaagccaatt tgctcataca acatgataat
     8821 ggacatacat tttttttgaa tgcgggacaa ttagggccaa atttccatac attgaccaat
     8881 gaaaaatatt taaaatttgc ttattctagt caatttggtt tttcgatacc gcgagcgaat
     8941 caattgaaag cagaaatggc aatggattca atgctaggga ttcagcgagc ggacacatat
     9001 atcacgacca gttttaagaa tgagagcatc aaaacctatg gacagttcat cgttcgaaat
     9061 aatgtacgag acattgtatg taagaaagag gtacttgcct ctacatggga acctacttta
     9121 caggcaaaaa tcagaacttg gcttattcct tttgaaggct ggcagattcg cgtacacaaa
     9181 gtagaattag atacagaata tgttctatat gagacagggt ttgctattga atgttctcct
     9241 gagaaagaag gaataatcat agaagaagac aaggaaaact atagggtgag tgaagctggc
     9301 ttttcaggta ttatctgttt aagtgacgat actattaaac gaaaatccac agctattatg
     9361 ggtttgccca atacaaactt gatgacatgg gaaaacactt ttattcctgg attagagggt
     9421 acatttggaa aaggaaccca ttggctcgga acaggagtgg ttgctcatca agatcgggat
     9481 tatagtttag aagtatggaa caatcgtccc aaaattgatt ttgatgggac aaccgggcta
     9541 actatccaaa ataaaaacaa taaaaaaaga ctaaaattat gctaaaggct ggtggggaaa
     9601 tgcttgataa agtgatgttt gaagagataa tacatccaga aaaatggata aatgaatgtt
     9661 ataaagaatt aatggaaaaa gaatggaata atgagatcga gcgttgtcgc agacacacgg
     9721 agaggttaat caaacgtatc agtaacagtc agtatcaatt cccaagcgag tgtcctataa
     9781 aaggacgtta tccattaaga gacaatattg gttggacaga aggattttgg acaggaatta
     9841 attggttgtt atttgagaag acgggtgatc ctttatttca acaaacagct gaaaaagcag
     9901 atatagattt tatccacagg attgactgtc aaattatcac taatcatcac gatcttggtt
     9961 ttttgtattc tttatcaacc gtagcagatt ataaaataac gggaaatcga aaaatgaagg
    10021 aagcttcaat tgatgctgca gaaacattac ttcagcgttt ttgggagaag gcaggagtta
    10081 tacaagcttg gggagatcct agtgatcctt cacaacaagg tagaatgatc atggattgtt
    10141 gcatgaatct tccattattg tattgggcga gtgaagaaac tggaaatcaa aaatataaaa
    10201 aagctgctca ggctcatatt caaaatgcag cagattattt agtaagagaa gatggttcaa
    10261 cttatcatac attttatatg gatgtcaaag atggaaaacc aagatatgga agtacgcatc
    10321 aaggttatag tgataattcc tgttgggcta gaggccaagc atgggggatt tacggttttg
    10381 cattaagtta tcgttatttg aaagaacaaa aatattttga tttaagcaaa atattagcca
    10441 attattttat gaatcggttg ccagaagata ccatttgtta ttgggatttg atctttgatg
    10501 attctagcag agaagaaaaa gatacatcta gtatggtgat catggtttgt ggattattag
    10561 aattaataga atcaggtcta ttagacaaaa cagaaaagaa cttataccag tctgctgttt
    10621 ggaaaattat gcgacaattg aaagaaagat atgctacaac ggaagaagaa gatggctttt
    10681 taacacagtc tgtttataat aagcccaaaa ataatggggt aaatacacct tgtatttggg
    10741 gtgattacta ttatttagaa gctttagtga gaatgactag taattggtct tcttattggt
    10801 gaaaaaggta aatatggaat ttttgtttga taaaataaaa aaagaaaaac caaaacagca
    10861 tgaatggttt ctgttttggt tttttcgtta attttcatat tcttctaatt ccaagcttag
    10921 ttcttcccat tccgtcaatt ttgtttcttg atcttgtcta gcagcttcta aatcttggtt
    10981 cagctcattg agttttacgt gatcttccag gttttctggc gcgaccattt cttgttccaa
    11041 ctgatcgatc cattcatcga tccgcgccat ttcttcttca acctgagtaa tttttcgttg
    11101 aaggctacgg ataagctttt gctgttcttt gctttggtaa taatcagatt tgttcgtggg
    11161 agcaggagca gattctggct catcttgtgc caacaattcg gcgatttctt cttcttcctg
    11221 ctttttctct aaataataat catagtctcc tagatagact ttgctgccat tttcagagag
    11281 ttcgacgact tttgtcgcga tccgattgat gaaatatcgg tcatgggaaa caaaaagcag
    11341 tgtgccgtca taatcgatca atgcattttc taatacttct ttgttgtcaa tatctaaatg
    11401 gtttgttggt tcatccaaaa tcaaaaaatt ttctttgttc atcgctaact tggctaaagc
    11461 aacccgtgct ttttcaccgc cacttagcag agggattggt ttttctacat cttcgccaga
    11521 aaataagaaa gcacctaaag cacttcggat ctctttttct ggagtggtag gatgctcatc
    11581 ccacaattct gctaggatcg tcttgtttgt atgcagatca gcttgacctt gatcgtaata
    11641 gccgatggtg acatttgtcc ctagcgtttc ttttccacgg ataaaaggga tctggtgaat
    11701 gattgatttc agcaacgtcg atttcccgat accattcgga cctactagag caatcgcttc
    11761 ttgacgacgg atatctagat tgattggttc agataaggtg acattatcat atccgatagc
    11821 ggcatcttcg atttgcaaga ctacatttcc agaaggcttt tctatactga atagaaagtt
    11881 tgcttgtttt tcatcccctt gaggacgatc cagccggtcc attttttcta atgtctttct
    11941 gcggctttgc gctcgttttg tggtagaggc gcgaacaagg ttccgagcaa cgaagtcttc
    12001 taatttatgg atttctgttt gctgcttttc ataagccttc cattcacttg ctaactgttc
    12061 tgctttcaaa tctagatatt tcgaatagtt acctttgtaa tggcgcattt ttcttctgct
    12121 taattcatag acttcattga ctactttatc taaaaaataa cggtcatggg aaacaatcaa
    12181 taatgcacca gtatagcttt gcagataatt ctcaagccag gaaagtgtct cgatatctaa
    12241 atggttcgtt ggttcatcta gaatcaaaat gtcagggtgt tgcaaaagca tgcgagctaa
    12301 agctaaacgt gtgcgctgtc cgcctgataa agtatcgatt ttctgatcat aaaatgaaga
    12361 atcgaattta aacccatgca atacggaacg aatttcattt tcataaccat agccgtttcg
    12421 atctgaaaaa tcgtgttgca gccggtcgta ttcttttaaa taagaactat aagcaggtgt
    12481 gtctggcgta ccttcgctaa tgaaaatctc tagctcgcgc atccgctctt ccatttgtct
    12541 aacttgttca aaagctttca gcatctcgtt ccaaactgtt tcattggaat caagtcctgt
    12601 gtcctgagcg aggtatccga gagtcgctgt tttattttta gcgatctttc cttcatcagg
    12661 tgcttcaatt ccagcaatga tctttaataa agtcgatttt ccagcgccat ttctgccaac
    12721 taaagcgaca cgagcgcctg tactgatttc taaatgtatg ttttcaaata agacttctgc
    12781 accaaaatgg cgagcaatct gattggcttg taataatatc atggaatcct cttttctact
    12841 ttgtgaattc tcctcaaata aaaaactatt tttcaattag tagtctacca taaattccat
    12901 tatactgcga gatttgcaga aatgataaat aaatgaataa agaataaaat ttcacaatat
    12961 ctgagcaatg gaattgattt tttcctataa atactgctat gatggagaaa tcaaagatgt
    13021 aattagacaa tacgtggagg tcatttcgtg aaagatcata ttattccaaa ggcgacagct
    13081 aaaagactgc ctttgtatta tcgctattta aggattttaa ataatgctgg aaagaaaaaa
    13141 gtgtcttcaa ctgaattaag tgaagccgtt caagtagata gtgcaacgat ccgtcgagat
    13201 ttttcttatt ttggcgaatt aggaaaacgt gggtatggtt atgatgtaga agatcttatg
    13261 cacttttttg caaagacatt aaacgacgat caattgacaa atgttgcgct gatcggtgta
    13321 gggaacttgg gaagtgcttt actaaaatac aaattccatc aaagtaatag catacgtatc
    13381 agttgtgctt ttgatgtgaa tgaggaaatc gttgggcgca tcgtagacgg tatccccgtt
    13441 tatccaatgg aagatatgtc agaacaaatc aagatccaac aaatcgaagt agcaatttta
    13501 acgatacccg ctgaaagagc acaagaagta gtcaatcagt taacggaagc aggaatcaaa
    13561 ggaatcttga actttaccgc tgctcgattg aatgctccgt cagatgtatt gatccaaaat
    13621 gttgatttga cgaatgaatt gcaaaccttg atctatttct tgcattctga cgcaaatgcg
    13681 cattccgaaa atacagcaga ataaaaataa aaagcgattg aacaaatgaa ggcaaaactt
    13741 tcacttgttc aatcgctttt ttctgtttgg acaacgatta ttttgttcgg aagatttctt
    13801 cataagaaag ggaatcatct tctgctgtca gttcatctag tagataacgg atttttttaa
    13861 agtcgatgaa ccgtgcttgc cgaaagactt cttttccttg atgaaaaatc aggacaactg
    13921 gcgcagtcag cacttcaaat ttactagcta cttctggtgt ttcaaatgca tccaaatgaa
    13981 gtgccggaat atcataatgt gtgagaagtt cctctagtcg tggacgaaca gcgtgacaaa
    14041 cactgcaatt aggcatcgaa agataaatga cggcaacgga tgactgttgc agtatgtctt
    14101 gaacttcagt caagttagtg gtttgtttca tttttattct cccttttatg atagaagtga
    14161 tatgtatgag accttaattt aatgggaata acagaaaaag tcaattgaag tgctttggta
    14221 aaaaataaga gttgtgtaaa aatttacaga gcaacttgtt ttttttgaat aaaagcgtat
    14281 atttgcaaag cgaatgaact agaaataaaa aggaggaaac tcatgttttc ttttattcca
    14341 aattcggttt tgcagatgag tacgattttc ttatcgatcg ttattgaagc attacctttc
    14401 gtattattag gctgtcttat ctcaggcgcc ttacaagtat ttttgacccc agaacgtgtg
    14461 aagagatggc ttcctgaaaa tcgtatcttc tctattttta ctggttcgat cttaggattt
    14521 ttctttcctt catgtgaatg cggcatcgta ccgatcgttc atcaatttgt taaaaaaggc
    14581 gtaccagttc acacagcgtt tgcttttatg ctgactgctc cgatcatcaa tccaatcgtg
    14641 attttttcca cttttatcgc atttggcaat tcttggcaga tggcaagctg gcgggtgata
    14701 ggcagctttt ttgttgccct tgtgattggt gtctggcttg cctattttca aaaagacgct
    14761 gtattgaaag aaaaaatcac gtctaaagta gcagaaaatc accatcatca tgtccatgaa
    14821 gaagaacaaa gtgagaagaa tgaaccaaaa aatcagctga tccatcaaac tgggcatgtg
    14881 ttgacacatt caatcgacga attttttgat acaggtcgtt atttgatcat tggcggatta
    14941 atcgcagctg cgatgcagac ttatttacca actagaatta tgctaacgct cggctcaaca
    15001 aagattttag ccattatcat catgctgtta ttggccttta cgatgtctct ttgctcagaa
    15061 gcagatgcgt ttataggatc ttctttgtta agtctgttcg gcacagcacc tgtagctgcc
    15121 tttttggtct ttggcccaat ggtcgatatc aaaaatctac tgatgatgaa acgatatttt
    15181 acaggacgct ttattttatc gctgatcgga ctgattgccc ttagcgttat tggattcaca
    15241 ttatttattt aggagggaaa atatgctgcg gtttttgatt ttatctgggt atttcgaatt
    15301 gatgatgtac ttgcaagttt ctggcaaact cgatcaatat atcaatgtcc attatcgcta
    15361 cttagctatt ctttcaatga ttttatctgc tcttttggct ttagttcagc tgtatttgtg
    15421 ggtcaaaaac ggaaaagcag aagaaaaggc acacgagcac gatcatgacc atgggttgtc
    15481 aaaaccttat caacgtggaa tcgcttatgt actgctagct ttgccagtaa tcgtggggac
    15541 gttgtttcca acagttagtc tcgacactac gatcgtagaa gccaaaggat ttaatttccc
    15601 agtaagtaaa gaatcagtag gggatccgga gatgcagaca caatacttaa agccggatac
    15661 cagtatttat ttcaacaaat cagactactt agaccaaatg aatgagttga aagaaaagta
    15721 tggggataag caaactatca agatcacaga tgaaaactat ttagaagtta tggagctaat
    15781 ttataattac cctagcgaat tcattggcaa aaaaatcagc tatgaaggat ttgtttacca
    15841 aacaccgaac gatagcaata cagatatctt cttgttccga ttcggcatta tccattgtgt
    15901 ggcagattca ggagtctttg gtttgttgac gcatatgccg gaaggagtaa cggttaaaaa
    15961 tgatgaatgg tataaaatag aaggaaccat ccaatcagac tattatcaac cattcaagcg
    16021 tgaaatccca tctgtagttg ttacaaacgc tgagaaagtc tctgctccga aaaatcagta
    16081 tgtgtatcgt tctttttaaa agaacaaaat aaataaacat agctcagaca gctgacacgc
    16141 aaggacgtcg gttgtctggc tttttttgca tgatgtattc atttgtttga tatttatttt
    16201 agaagcagca tcctgaaagt atttgatatc ggtttcaaag gtgttggaaa agattgtgaa
    16261 gagaattaca atttattcct aacatgtatg ttgaaaagtt gtatgaaatg attttgatag
    16321 taaaatatcg agtggaggta ctttttatgg taacgcgtat atttattttg gatgatagtt
    16381 tggtttttga aaaaatgcta gtagaaattt tggaagaatc cagaatttta ttaagtcctt
    16441 ggaaatttga gatcgttcag ggattgaacg ttatacaatt tgttgaattc attaaaaata
    16501 atgaaatcta ttcctcagat atttttttcc tagacatcga tttgaacgcc tattatagcg
    16561 gtatcgaact aggcgaaatg cttagagaaa agaatgaaaa gtgtaaaatt gtttatctaa
    16621 cgtctcatcc gaataaagca ataactgtta ttaatcgaaa gattacgcct agtggttatt
    16681 tgataaaaga attcacttat gaaaaaaata aaaaaagcat ccaaagttac ttattgaaag
    16741 aaaaagaaaa ctatcctgtt caacatgcta cgaaaaaaag gtggacaact gtccgttatg
    16801 gtaatagcga tcactatatc cattacgatg aaatccttta tctaatgagc atgccaggtt
    16861 ataaaaataa aataggccta gtattgaaag atgaagagat tctcgtaaac ggcacaatta
    16921 aaaaatataa aaaatcactc gaatatgagt atttatgtaa agatttaaaa gcttatatca
    16981 ttaatactga aaacattaca tctattagca gagtaaacga aatagtaaca tttgcaaatg
    17041 gagaacaact agagatggga aagagaagta ttgataaact aaagaatttt atgcaagaaa
    17101 agatagaaaa tgaatcattt taaagaaaac aaagactcat atcttacctg ttagaagagt
    17161 gttttcctgt agaagagaac aacccaagta gcagcctagt cgtttctatc cttttttgtt
    17221 atgcactctt tttgacattt actccaccaa taacgaaggg tacaataaaa agtaataaca
    17281 aacaaaagga gtacagaaat gattgaattc atcaatgtaa gtaaaagtta cgggaaaaaa
    17341 caagcattaa aagacttgtc tctctcgata agacaagggg aaatttttgg atttttagga
    17401 cataatggtg ccggaaaatc aactacgatt aaaagtttag ttagtattat tgaaccatca
    17461 tcgggaacga ttttagtaga tggaatgaaa ttgacggaaa atcgattgag tatcaaacaa
    17521 aaaatcggtt atgtgcctga ttccccagat atttttttac aattgacggc tggagaatat
    17581 tgggacttga tttcagctgc ctatgaatta gacacacaaa aaaaagaaaa gcgtttagca
    17641 gaattgacag cgctttttga tatgtatagt catcaaaatg aaacaatcgc aagtttttca
    17701 catggaatgc gtcaaaaaac gattttgatc ggcacgttgc tgccagatcc agatatttgg
    17761 gtattggatg aacctttgca aggacttgat cctcaagcag catttgattt gaaagaaatg
    17821 atgaaggctc atgccgcaaa aggaaaaaca gtcatttttt ctacacatgt attggatacc
    17881 gcacaacagt tatgcgataa actggctatt ttgaagaaag gtgaattgat ttatcagggg
    17941 gcagttagtg aattattgaa tggctcacct gatgaaacgc tagaaaagat ttatttgaaa
    18001 atggctggaa ggcaagcgac aggagatgga ccgtatgtat aagcgacaat tattagaatt
    18061 aattcgagtt aatcttttat atgttaatcc acagctcacg aataaagcaa gagagaaagg
    18121 aaaaaaggga aagcgattaa ctatctatct tttgtctcaa aatctttttt cagggctgat
    18181 ttttttactg atttacggat ttgctatgtt tgctatgaac tttgttaaat tgccaggttt
    18241 ctttacttat tacgtggcac tttttggtat gttaggttta actcaaagta tatcagtcat
    18301 ttataatatc ttttttgaag gaaatgacct gcaagtattt ttgcctctgc cttttggaca
    18361 agaacaggtt ttttcagcaa agattttggt agttgctttg accatcattc cttttgtttt
    18421 tccgcttcta gtcatgttta ttttaaccgg atggcgagct gatctaggga ttttgcttac
    18481 tatcattatc tcaatccttt tgttttctct atttttgatt attctttttt gtctggctag
    18541 tttgattgtt tttggtttgg ctcgaacagc agtgtttaaa aaacatagaa aaacagtttc
    18601 tagtatcttg cttggcagct cgatgctgat cgcatttgct ggaatcatgt ggatgaatca
    18661 tcaaacgggg actattgaaa atgagttaac agatcggcat ccgatttcga tattattgcc
    18721 attctatgat gctgccagtc agccactttc tcaacaaggt ttattgggtt ggggaatact
    18781 agctatcacg gctctgggat ttttccttgc tgtaaaacag ctgatagtac caaagcttac
    18841 agagcaactg actgattcaa acagtgagtt caaaataaaa agaacccata aaaagaacca
    18901 aaatgtgcat cagcttttat ttagttacaa tgctcaatta gtaaaagaac ctaatttgat
    18961 tatgcaagtt ctttcttctt ccttgttaac tccaatcatt tttattcttg cttttgccct
    19021 aggaggcgaa ataaaattaa ctgatttaga taataagtta atcggtgttg ttttcctggt
    19081 agggatcgca ttggctttct taacagttaa tcagacttct tttatcagca atcttatttc
    19141 tttggatcaa gaaaattttc tgtttatccg ttctttgcca atctctatga accaatattt
    19201 aaaagaaaag ttcagattcg gatggatatt gcagagtttt ttaactggtg ggatcgcatt
    19261 attaagcgga ctgtcttttc agctgccttt gttttttatc cctagcttgc tatttggttc
    19321 tttattcggc tgctacttat tgtcactacg ttatttcgct agagattatc ggttgctgct
    19381 gctgaactgg accaatatta accaattatt taacagaggt tcaggaagtt tgggtttagt
    19441 cgcaactatg atgggttcat tgatcatcag tattattctt atcgtcttat acggaatgtt
    19501 ggctttgttt tatccatttt ggttagtgaa tattcctgtg atcggtttag ttctaatact
    19561 ttccgctcta tggatcgttt attatcaacg aacgttttgg aaaaaatttg aataaagaag
    19621 aggggccgtg acaaaaatca tgtcgcagcc ccttttttac agatagaggg tgttcaaaag
    19681 tcagtttctc agtattaagt caattttatg taaaaatgtg agaagctatt tttacaaaac
    19741 tttttttaat actcataatt acttcttatt gctcctgcgg ttacttgtcg caatgtttgt
    19801 gagatatttt gtcatcgctc aaggctgaac gtctctttca caaccttttc tttgttacac
    19861 ttcgtttatt taaaaagccg tttttttcgt ttcatcatga ttgcgtcacg ctttactttg
    19921 aaattacgga tgttatggtt aaagacttct gataatacaa gacctaaagc gattgctcca
    19981 gcaatcataa ttgcttggac cgcataatga gccgcttctc gataggattc agtaacgaga
    20041 tttcgaaccg cttgataagc taaaccacca gggactagag gaaccatccc gggaatattg
    20101 aaaatcgtta cgggcatttt caaacgttta gagaaaagat cgctgatgaa tgctactcct
    20161 aaagaaccaa gcaatgaacc aaaagcagca gatccgccta tttgaacaca gatccagtag
    20221 atcatccagc caaaagcacc agaaagacca caacaaatca atgaacgcct aggtacattt
    20281 gtaataacgg caaatgccgc agtcgctaaa aaactaaaag aaaactgaat aaatatcgtc
    20341 cccatacttg tgttcctttc ctaataaaaa agctggaaaa taaaagcaat cgcaaaaccg
    20401 atcatcgtag cagtcatcat cgcttctgtt ccgcgagaaa ctcctgagac ataatgtcca
    20461 gctagcaaat cacggatagc gttcgtaatt tgaactccag gaactaaagg catcacacat
    20521 ccgataatga ttagatcctg attgcttcct aagccaaaac gtacgctgta gatagcggct
    20581 atgccaatca ataaagaaga taaaaattcc gataaaaatt tgattctaaa aaatttcaaa
    20641 ctatatagat aagctgcgta tcccaatcct ccaatgacta aagtcacagg taaatcggtc
    20701 atctttccgc caaataacac cataattgtt ccactgatca ctgccgcgct aacgatttgc
    20761 aaccagacag gaaagaaaga acggtctcgg tcgattgctt gcagttcgca gtaaagttct
    20821 tctaatgtta gctctgaagc aacatattta cgtgaaagat catttacttt tactactttt
    20881 tctaaattaa tcgaacggtt ggtaatttgt tccattcgga tggtcgaggt acgatcaaga
    20941 ccaataaaga gaccggtttg agtgacatag ctgaccaaac ggaaatttcc agaagctaaa
    21001 gcaattcgac tcatcgtatc ttctacccga tacatctccg cattgctttc catcatgatt
    21061 ttcccagcga gtaaacaagt gtccagcaac agatctaatt cttttcccgt catagcatct
    21121 tctccaataa ttgattgatt agttttattt tactacaaaa cataccagaa gcgtaaagaa
    21181 taaaaaaact tgaggaagga ataaaagagg cgtatactgt ttttgtattg cgggtgtagt
    21241 ttagtggtaa aatcccagct tcccaagctg atgtcgcggg ttcgattccc gtcacccgct
    21301 taaaataggt tgtgaaaaag ctgctctgca acaagtaata agaacagcca agcaaaaaca
    21361 acttcttatg tttttaactt ggatggactt attaccgcag atgcaagctt ttgcgcaccg
    21421 tttaccaagc ggttgtgaaa tcagtgtatt gtcttgagta tgaagaaaat ttttgaataa
    21481 taataaatac cttacagata gagtggtgac tgattagaga cgaggaatcc gtctcttttt
    21541 tgtattttta agccatctta aaacaggttt tattttttgc tgtttctatt tctttctagt
    21601 atttatttat aggggaacat atgttaagat ttagggagct gttagtaaag gagtaaagaa
    21661 atgagcaaaa aaacaaccaa ggcgatcgtg gggatcgtcg ctgctgtgat tgcaggggca
    21721 ttcggtgttt ccaccacaca aaaagattca gatatctttc agcagattac aactattttt
    21781 gattccacac cacaagcttc tcaagtagcg gatgtgccag aatatgatgg aaaacatcaa
    21841 gaaatagaaa tcaatgggaa tcagccggaa tttacaaaag aggatcttag cctttccaaa
    21901 ggaacatggg aaagctattc aaatattgat aaattgaaca gagtaggaca agccaatgct
    21961 atgcttggaa aagatttgtt ccctactgaa aagagagagc ctttgtatat tgacccgaca
    22021 ggttggaaac aaaagaaatt aagtgacggg caatggttat ataatcgcag ccatttaatt
    22081 ggtttccagc tgactggtca aaataacaac attaaaaact tgatgacagg gacacgttcg
    22141 ttgaatgctc catacatgct tgctcacgaa aatgatattg cggcatatat caaagaaaca
    22201 aatcatcatg tacgctaccg tgtcactcct cattttgagg ggaatgaact ggtagccagg
    22261 ggagtgcaat tggaagcaga aagcattgaa gacaaaaaaa ttgaattcaa tgtctttatc
    22321 tacaatgttc aagatggcta cacgatcaat tatgagacag gacaagcaac aaaaacaaaa
    22381 aattagtagc agctattcga attttttatg aatattatga aaaaattgtg taaaaacata
    22441 cattcaaaga aatatagcgt atactttatg aaaagagtac ttttgataaa gctacagaaa
    22501 aaaggaaaaa aacatacaaa tagtgtttct taatttattc ggaagtttgg aatatttgtt
    22561 tgtagttttc cctttcttcg agtaaaataa ggttgtagaa ggtaagctgg aattaccttg
    22621 cgggaaagag gtgtagaatc atgggtttta cagatgaaac cgtacgtttt ggttttgacg
    22681 atagtcgcaa aaaagaagtg agcgaaacat tagcgattgt ttatcgggct ttggaagaaa
    22741 aaggctacaa tccaatcaat cagattgtcg gctacttgct ttctggagac ccggcctaca
    22801 ttcctcgtta tcgtgatgct cgaaatttga ttcgccgtca cgaacgcgat gagatcatgg
    22861 aagtaattgt caaggactac ctctcaaatc atggagtgaa tttatgagag taatgggctt
    22921 ggatgtcggt tctaagacag taggtgttgc tatcagtgac ccctttggct ggacagctca
    22981 aggagtagag atcattcgga tcaatgaagc agaagaagag tttggctttg atcggttgaa
    23041 ggaattagta gcacaatatg aagtagaccg gtttgtagtt ggattaccta aaaatatgaa
    23101 caattccatc ggaccaaggg cagaagcttc catgtcatac ggagaaaaga tcaaagaact
    23161 attctccttg ccagttgatt atcaagatga acgattaacc actgttcagg cagaacgtat
    23221 gctagtagag caggcaaata cttcaagagc caaacgaaaa aaagtcatcg ataaattagc
    23281 agctgtgatg atcttacaaa attatttaga cggctcaggt aagcaattat tttaataata
    23341 aaagaaaaga ggtaatctta tgacagaaca taaccattcc cacgaccatg aaggacacga
    23401 acacatcaca ttggtggatg accaaggaaa tgaaacatta tatgaaatcc tactaacagt
    23461 tgacggacaa gaagaatttg gacgtaacta tgttctttta tatcctgctg gtgtttcaga
    23521 agatgaagat gtagaattac aagcttatgc atacatcgaa aacgaagatg gaacagaagg
    23581 cgagttagaa caaatcgaaa cagacgcaga atgggacatg attgaagaag tattcaatac
    23641 attcatggcg gaagaagaag aatagactat aaagctagga gaaaccttga tatgataggg
    23701 tttctccttt ttttattttt tactgtatgc catattgtat gccatctagt caatatttaa
    23761 gtgttgtact aatttttcaa cagtcttttc tttcgtttct tttgccaagt gaccgtaaca
    23821 atcgatcgtc tgactaattt ttgtatgccg taatctagtt tgaacttcta ccatagtagc
    23881 tccagcgtcc aagagcattg aagcatgagt gtgtcttgct acatgcacac cgagaaatgg
    23941 aaccatagct ttttcacaaa tagtaatcaa tcttgcataa atagcgtggt gtgtgattag
    24001 ttctttatag ataccacaaa aaatcatagt cggatcagtt acaccaagtg tcagcatata
    24061 tttccgctga ttaatctgcc atttttttaa tatagaaatt gttctttcat ctagtggaag
    24121 aaccccagca ctacaatcag ttttaggatc actgatttta tagccatttt tggattggct
    24181 tagtgtttta ttgatggtga ccgtattttc ttgaaaatca atgtctgacc aattcaatgc
    24241 aagggcttca ctgatcctaa ccccgctata aaagagaaag cgtagtaacg ttttatcaaa
    24301 gtcgtttcga taagtttcag gtttgctgtt taggtaatca aacaaggtga tcagttgttc
    24361 tttactgtaa actttgacct tttctttatg cttagtggtg gttcttttag gcattaatac
    24421 gttgtctagt ggattagtct caataatccc aagattgata gcgtagctaa aaatacgctt
    24481 agaagttgaa tgaagtacct tgtattggtc agtagtttca gcccagctat ttacaatttg
    24541 ttgggctttt tttaccgtga cttcattcac acgaagattt ccatattttg gaagaatcca
    24601 ctttctaaac ttgatttcag ttttcatgaa ggtggcttct cggacagtgg ttttatatgt
    24661 ttctagccac aagtcataaa cttctttaaa cgtttcgttg ttcgcttttt taataccgtt
    24721 tttatcgtag tccacttgca agcgtgacaa ggcaagctgg gcttcttttt ttgttttgta
    24781 acctcgtcta cgaaccttat caggtttgcc agtcattttg ttcattccaa gataggcttc
    24841 atagagccaa aatttacctt ttttagtttc atattgcttg aatgttgcca taatagtttt
    24901 ccctccatag tgtagcaagc cgtgggggcg gttggaggag gcagtttatt attattttga
    24961 atagtgttta tttagctcag taacaagagc tgtgaactct tttagaagat cattttcata
    25021 aaattcttga tactcttttt tctcatctgt atttttttct gaatcattaa ttaatgagtc
    25081 atatcgtaaa agcgtattta gaagagctgc gagataagca attggggtac tcccgtcagg
    25141 aaattcaatt cgagatcctg ttttaagata ttttattgcg tttgttaaat aaatcttctg
    25201 tgagacagtc aggttatctt cttcgatgtt attgatagca ttagaaagat tagaatcaat
    25261 tatagagtta tttttttcaa tagttttttt caattgcttt tcaagcacat ttcgcttaat
    25321 ttcgggcatc atatgcatat ctcttaatgt ataagatccg tttaatagat aggtagtgga
    25381 tacatctaat acctttgaaa gttgtttaag cctggtgtta ttgggaagat tatagccatt
    25441 ttcccaatta ctaacggcac ctttactggc cggtggggat aatttacctc caagttgttc
    25501 cattgagagt cccttttcaa ttctagccag tctaattctt tgtcctgtct ccactgcttc
    25561 ggggtatttt tttctagcca cattttcacc accattcata tattttctct actatatcac
    25621 gatttaaata tgtgtgaaac ataaaagttc aaaaaagtat gttaaaattc ttgcaaatga
    25681 aaaggtatga tggtatattg agtttataag aggtatagaa atgtattcaa gagcctcttg
    25741 cattttttta aaatctttgt atagaaatgt atgctaacga ggggggataa atatggaatt
    25801 aaaaattgac aaactcacgc ttgatgatcc agcttttaaa attattgacg atcgagttgt
    25861 tcaacaagta gagcaaactc aaggaacgtt actttcatta ttagaaaaaa agtaccagtt
    25921 tccaaaatat atgaacaagg cacaagctgc taagtatatg aacgtttcat acaatactat
    25981 gatgcagaaa tatgttccta acggtttaaa gttgattata gttgatggag tcgtaagaat
    26041 ttcacaagat gaatgtgatc gctttatgga agaataccaa aaataaacgc aagccgtggg
    26101 ggcaaaggga ttaataggag gaaataataa tggaatcgac acaaaataaa gcaatcgaaa
    26161 aagtattatc aacaattctt acagaagaca cagcggcaga attagcaaac ttggaaggca
    26221 aagcgctaga agaaacgttt gaatgtttgt acgaacaaat ggactatcaa aaattgctac
    26281 cacaagaacc aacggtaagc ggagtattgc gtggcttgaa cgatttagta caagcagagt
    26341 ttaaagaacg actttctatt gaagagtatc aagaaatttt gtatcagcaa gttgagttgt
    26401 tagcgagctt gctagggctt gaactggagg acgttgaatg aaaactgaaa tttggaacgg
    26461 acacattata agatttgtcg atattaatga tgaatggtgg gcagtggcga aagatgttgc
    26521 ggaagcgtta ggtcttaaac aggttactag agccattcac tctttgccta aagatggggt
    26581 tactactagt aaggtcatcg attcattggg cagaacacaa gatgtaaaca tcatcaacga
    26641 aaaaaaatat ttaccgcatg gcattcaaaa gccgtaaaaa agaagccgag gcatttcaag
    26701 actggatttt cgacatcatc aaagaactac gccagtccac aggacttgaa ggctttcaag
    26761 tattccgcat gctagacaaa gaacaccaaa aagaagcaat gactaaatta agccacgcta
    26821 ttactgaacc aaaaccagta gactacatta aagcgaatgt gatagccaat aaagctgtat
    26881 caactattta tggtcattcc aaaatggtaa aaaagaaaga tatgactcct gaaatgttgg
    26941 ttgatcgtga acctatttta gatgagacag tagaattaat gactgttaaa gaaaaatatg
    27001 ggttgcagtt ttcagtgagt gagaaaattt ataatcgttc tgctgaattg cagacaactt
    27061 aggagcatta acatgatcga gaacaaagaa aaagccttat ccataacagt cttggcggac
    27121 aggataaagg caacttaatt gggataaaac ccgatattct ttgttccgat tataccatat
    27181 taaaaaatcg gagggaacca ttatgaaacg aaacttaatt ataggaagca ttttaatcgg
    27241 cggaattttc ggcttattac cattaattgc aaaaatagct atctttgcag gattagcgtt
    27301 atatctcttc actgcttatg acgagtacga ctatcaatta agaactggag ggcataagta
    27361 gatgtacaaa acaaaactat taaaccaact cgatagcctt gaattagagg aaattaatca
    27421 agggatcgct gaactagaaa ataatatcgg gaaaacctac tttggaaatt cattcaatga
    27481 aaaactaaca gttttgtatg tactcaaaaa acatgcagaa cacaaaataa tttgtcgaga
    27541 aatcaacgag ctaaaaaatc agattttaac cgcatggtta aacattactg atatgcaaga
    27601 agcaagggtg aaaacattta atacatgggt aaagtatcag aatcaattaa aaggtgctga
    27661 atttgttcgg gatgggttga aatacgaact tgagcagttg aagctcatgg aggtgtctga
    27721 atgatttata ccagtatttt gaaaaaggag gagaggttta ttggcaaata attatcaagc
    27781 agcaacggaa ttacaaaagt cgggatggtc aatttatccc ttatctcctg gaactaaaat
    27841 tccaataaaa ggctctagtg gttggaatga tgcaactaac gattctaatc aaattaatga
    27901 atggtggacg gaagatgcaa ctagaaatat tgggttaatg cttgaagatc atcaaatggt
    27961 ggtagttgac ctagatcaac attctgaaac acgaaacggc gttgagaatt ataaaaaact
    28021 tgcttcaatg tatgatccat ttccttctac ttatacggaa atcacaccag gtaaaggaat
    28081 ccatttcttc tttaaaattc ctgatggtgt aattatccaa caagaaacag gtgcatttgc
    28141 tgatatcttc gggcgtgatg ataaaggtaa aagtttaagc gggattgata ttatcactaa
    28201 aggagttcca atagcgccaa ctatgattat ttcaagaagt gttgggagag catatgaacc
    28261 tattgaaaac gtatctctaa ataatattgc accagctccc gattggctag tgaacctact
    28321 gctaaaaaaa ccgcaagaaa taaagactca ataccgaccc gtagcaaaaa cagggacagc
    28381 aaaaaaatta gatatgattg ttcaaggcgc tggcgaaggt agtagaaacg atcatttaac
    28441 taagttatgc ggttggttac tatggcacgg tgtagataat caaactttag cagaattgat
    28501 ttatacagca aatgcgtaca atgaaccacc gctaaaagat aaagaagtga atcaaattat
    28561 tcgatcgatg attaaaaaag atttgagggg gaaagctcat ggctcataag cataaattag
    28621 atagtatttt agactttccc gaagccagcg agcgtgaaga caatatcatt gaattgaaaa
    28681 cctggatgtc acgattaaga tgcaataaag acgatcagat taaaagtaac agtgttgtga
    28741 atgcagaatt gattttaacc aatgatagca atttagctgg aacaattgct tataacgaat
    28801 ttagcggata cattcaccta ttaaaagatt ctccttggat caatcgtacc gctggagaat
    28861 gggaagacag ttttgaggat gccttaacgg catatattga agaaaactac aatgtagttt
    28921 ttgatgacaa caaaatacac aaagcagtag taaatgtggc tagaaagaat gttttcaacc
    28981 cagtaaaaga acgtatcgaa aaagttaaat gggatcaaca gcctagactg gaaacactgt
    29041 ttattgacct tttaggggta gacgacaacc tttacacacg agaagtaaca aaacgttgga
    29101 tagtcggatc agtcgcacgt atctataaac caggaataaa gtttgagatc gttccagttt
    29161 tggatggacc gcaagggatt ggtaagagta ccgttccagc attactttac acggatgatt
    29221 ttttcaccga ttcattagat tcgctaggtg aaaagaaaga tgattatatg cagcttcaag
    29281 gcaatgtaat tattgaactt ggagaattat cttcaatgaa caaaactaaa attgaacaga
    29341 taaaaaactt catcagtgcc aagatcgata aaattagacc tccttatggt cggaatgtca
    29401 tagcttgggc aaggcaatgt gtatttattg gaacgtcgaa tgatgggcag tatttgaaag
    29461 atgatacagg aaaccgccgt ttttatcctt tgccttgtaa gaataagcca aagatgaatc
    29521 cgtttaaaac aaaggatgaa tattttcttc aagtgttagc tgaagcaaag gtgctttttg
    29581 ataaaggaca acgaatctat tttagtcccg atgaagataa agaagtctta gaaattgcaa
    29641 aagactatca agaggatgcc aagatggaga atccaattaa agaagcaatc actaaatatt
    29701 tggaaatgga aatcccgtat aaatgggaag aagctccagc gtgggcgaga cgttcttatt
    29761 atcagaacta tcctgacagt aaatgtaatg agaaaaattt acagcatttc tataatggag
    29821 ctactatgca acaagagttt tatttagttg atagcgtact caccgctgat attttggaag
    29881 ctgtttttga taaacaagca aaggatttat tgaatggtcg atcagatgca gaaactaaaa
    29941 agattgcctt gattattagc agtattcctg gttgggaacg gaagcaactg ccaaaaagaa
    30001 ataaacgacg aggattttac aataagtcaa atgcctatga aaataaaaat cgagctggaa
    30061 agtacaaaaa gtaacgggta gtaacgggtc agtaacacct agcccgttac tagtttaccc
    30121 ttactgcccc aagggataca gcaatttggt aacggggtaa caccttattt ccttaaaact
    30181 tttgaaatag ttagctaata tgctggggaa taatgttact ggattttacc cgttacctgt
    30241 taccaaaacg ctagaaccct tgtccccgtt gggattgagt ggtaacgggt acctgttacg
    30301 taggtgttac cacgtgttac gaatcaagga ggaaagtaaa tgctcaatat tatttcaaca
    30361 aacaaagcac cgaactttca atatacagat gaaatggatc ggtttctaat gaacacgctg
    30421 gcatttagtg tgggattggt aacagaagat tattccacat ttgatccaga agtattaaag
    30481 ataatggaag aagaacctga ttggctgcag gagtcagtag cgtggtgtca atcgctagta
    30541 gtgggatcat tggtagatag tggcaactat gatgatacag gtgagctaat ggatgaattt
    30601 aattgtctgc ttaacctata tgatcgagcg agacaacgag agcttacatc gaatgaggat
    30661 aatttgtttt taaacatcca tgataagttc ttggctttgc tactaacaga tgatgaattg
    30721 ataactaatt tattggaggt agaataatta tgcatatgga aagtattaga tacttcgatg
    30781 gtgagaaaca cattgagtat tcaaacactc aagaagatgt agactttatt agttttgagg
    30841 aagataaaat cactaaagtt aattttaaag acggaacgtg cttgaagata gtttcgcctt
    30901 atattgaata caaatcaaaa tgggaacaag atgattctga cgaacgagat cgctttggtt
    30961 ggtaatctat ggcagttaaa aaacaatgta atcatgctgg atgtaaaaca ttgattgatt
    31021 ataggcagaa gtattgtggg aagcataaag caaagcaaac ggcaattaag cgtgaagaaa
    31081 gaaagcaaag cgaaggtaaa tattttcaat tctatcaaag tagaacatgg cgaaaggctt
    31141 catacttgta tcggttaaat catccagtat gtgaggattg cctagaagaa ggcttgataa
    31201 ggaaagctga tgttgtagat cacaaaattg aactaaaaga tgattggaat agacggttag
    31261 atgaaactaa ttttcgttct ttatgccatg cacaccataa ttccaagaca gccaatgaaa
    31321 gacaaagacg agaaaagagt accctttgag ttaagggggc atagtgtgaa acttactgac
    31381 aatcgatgcc tactcatttt ggtacaaata accgttgtga aaaggcataa gggtaattac
    31441 aaataacgat tacacttgta atcacaagtg aaaaagtata taatgaaagt aggagattta
    31501 ttgaaaaata caattgttat tactgataaa acagggaaac agcgcaccat tgagttacct
    31561 cattttggtc aagtgaccat tcaaatgcag aacggaaaaa tcatttatat agataaatta
    31621 gataaagaaa aattctgatc gaaaaacgaa ggaatgtgag cttaattgct tacgttcctt
    31681 tttcttttgt ctgaaaggag gacaacatgg gacaccctaa aatattacac gatacaaaag
    31741 gcaatatatc aagtgaagaa aaagcaatcc gtgtggacgc tagagaagaa ttgttcaagc
    31801 agcaaccatt aataaatatc acgccccctg actggatggc agcgagcgct agaagtgaat
    31861 ggaatcgtat cgtaccaaca ttaaaaaaag attatccatt gagtgaagca gattatggtt
    31921 cattggtagc ctattgttta gcctttgctc gaatgaaaac agccgaagcc gagataagaa
    31981 aatcaggtac gtttatcaca tgcgaaaacg gagtaaagaa agccaatcca gcggttcggg
    32041 ttcaatcaca agctatgagt gatttgaaaa aacaagccac ctcactaggt atgaccttag
    32101 aatcacgatc aaaactagct ttgaacaagg ttaaaaatga tgaacccgaa gacccgttca
    32161 aagagttgat gaattcatga atgattacat tgagaaagtc ctatcaggta agctgattgc
    32221 acccaagaaa attatccaag cgtgtgagcg tcatataagc gatttggagc gttctaagtc
    32281 agatagcttc ccttatgtgt tcgatgaaga acaagccacc aaagcgatta aattcattga
    32341 gttgctacca tctacggacg gtaaagcaat caagatgtta ggatttcaaa aattcattct
    32401 aggcagtctt tatggctggc gtactaaaga agggaattac aggcgattca atcgagcgtt
    32461 taccagtatg agccgtaaga atgggaaaac gtatatcgca agtggcatgg ctgccaatgc
    32521 gttgattatg gaacaagaac cagcagaagc aaggcaagta ttgtttgtaa gtaacgcttt
    32581 gaaacaagct aaattgggct atgatatgct gtctaattca cttagaaacg tggtcaagtc
    32641 tagtaagttt ttaagaccac aactgaaaat tatgaactct aagattcagc acttgccctc
    32701 taattcgttc gctatggcac tggctagtga aaccagcacg ctagatgggt ttgcaccaac
    32761 aaccgcaatt cttgacgagt ggcacgaagc aaaaactcgt aaaacgtaca acgtcattaa
    32821 gtcaggaatg acccaacaaa agaacggctt attgtgtgtt attagtaccg ctgggcttga
    32881 tttaaacgtt cctatgtacg aggaatactt attgttagat cgtgtgctaa aaggcgaaga
    32941 acaagccgac aggtacttta tagcgatatg ggaattggac gatcccgaag aaattcacga
    33001 tcaagagaaa tggatcaaag ccaatccgat ttttgaaagt gaagaaatca aaaaagtaat
    33061 gattccaacc attcaagatg atgtgaacct tgctttgaaa caaaataacc ttaattctgt
    33121 attggtgaaa aacttcaatt tatggagaca agcgagtgag gacagctata tgattgctga
    33181 agactggcag gcaaccgaag tagaaccaca agatattaca ggcaagcccg tttatatcgg
    33241 agtggattta tctaaaacag atgatttaac tagcgtttca tggatcgtac cgctagataa
    33301 cggcgaactt tattgtgatt cacatagctt tgtagccacc aaatatgggc ttcaagacaa
    33361 agaaaagcgc gacggtttgc cttatcgaga actagaaaaa gctggtgagt gttccattac
    33421 tcaattagaa agtggaatcg tggactatga ccaagtattt cagtttattc aagatttgat
    33481 tcaagaaaat gatttggaat gtatggggat ttgttatgac ccgtataacg ctaattcgct
    33541 tatcagtaaa gctgaaaaag ccaactaccc aatgttagaa gtaagacaag gaacgattac
    33601 tctaaacgtt ccgacccgaa cttttagaga acaagtttat gaaggcaacg ttattcacaa
    33661 taaaaatacg attctcaccc atgcagtgaa caacgctatt ttaaaaacgg ataacaacgg
    33721 cattcagatt aataaatcaa agaacagtaa caaaattgat ccaatagctg cattaatcaa
    33781 tgcctatgtg tttgcaatgg attacttcac cacaacggaa ggagcgaaag cagacaatga
    33841 attttataca agtgaagaat tttctttcta attacattca taccgttctt ttacttcttg
    33901 gattggtgtg tgtgttggtt gctatcacct tactaacaaa tgtctactat ggcttgttag
    33961 cgctgggcat agtgcttatt ggggtagcgg tcatgctaaa tacagaacag aagggaggtt
    34021 aaaagatggc attttttaaa gcgagacaaa atacaacggg agatcctttc ctacaacatg
    34081 tggtatcaat ccaatcggat gattacacca ccagctttac aagcgttcgt gcattaagaa
    34141 atagtgatgt gtttgcagcc gttcgaatta ttgctagtga tattgcttca agtccgattc
    34201 aattggtcaa aaacaatatg ccacaagctg ataatgaact ggtgaagtta ctaaacgaga
    34261 aacctaattc agaaatggat ggttggcatt taaagtttgc tttggcggtc aatatgttgc
    34321 taaacggtaa tagctttgca gagattaaac gcaacggtga aaaggtagaa gaaattcact
    34381 tattacctaa ctcaagtgta acggttactc aattagataa tggtacgttg tcttatcaga
    34441 ttggtgataa aaaaagacgt gtgaagtcta gcgatatttt gcacttcaaa tatttcaccc
    34501 aagatggttt aacaggatta ccaccacttt atgcgttacg tgatgaactg aaaattcaac
    34561 aagctggcaa ccgcacattg cacaatttct ttactcgtgg tgtcagtggg tcaggtattt
    34621 tgaaggttca taagtcagat ttagacggat cagcaaagaa cgcaattcgt gaaaagtttg
    34681 aagaagccaa cggttcaagt agtggagata atgctcttag aacgatcatt cttgatgaaa
    34741 caatggacta caaaacatta gaaataaaca ccgatgtttt gaagcttatc aattccaatg
    34801 attggaacac aaaacaaatt gccaaggcgt ttggtgtacc gattgagcgt ttaggcgttg
    34861 aaaatgaaca ttctagcacc gttcaaagta atctccagta tatccaaagt acgctgattc
    34921 attactttaa tgtgtttgtg agtgaattag atacgaaact tgaaaccaat atccgcttta
    34981 attccgatca gttactagag actgatcctg aaacaaaagt aaaaaatgta ttggatcagg
    35041 tcaaagggtc acttctcacg attaatgagg ggcggtcgaa aatggggcta ccccccatgg
    35101 atggtggcga tcgtttacta gcaagtttga actttacgta tttagatacg ttggagaaat
    35161 atcaattaaa agaacaggaa ggagttacac cagttgaata atgaagaaga aaaggaaaaa
    35221 cggctgacag aagaagctga gctaacagcc gattctccca aagtagagaa agaaaatgaa
    35281 aaacaaccaa cagacggcaa aactatttca ggctatgcgt tgaaattcgg gcaaccgtca
    35341 aaagatttag gcggctttgt agaagtgatt acacccgaag cattaaaaga ggtggattta
    35401 tcaaatgtgt tcttattata tggacacgat tattcaaagc cactagctag tgtcaaagca
    35461 ggtacgttga aattgaatgt cgatgataca ggattacatt ttgaagcaga gctaaccgac
    35521 acgacctatt caaatgatgt gtatgagaat atctcaaaag gggttgttga ttctatgtcc
    35581 tttgggttcg tgcttggatt agattcattc gacaaaaaag aagatggcac aattgaacga
    35641 tcaatagata aaatcaaagc acttaatgaa attagtgtcg tgaccgttcc cgcttatgat
    35701 tcatcaaatg tccaagtaaa taagcgttct tacgaatcgt ttatgagtaa caaccaagca
    35761 aaacaaacaa acaatagctt agaatccact tctaaagcac aaaaggagag taataacatg
    35821 gaaaaaactt taatcgataa cgaaaaaaca gaattgcgtg ggtatgaaga atatatccgt
    35881 tcacaaggcg aagtgcgtga tggagtcact actgtaaatg cagcggcagt tgttcccgaa
    35941 gaagtaatcg gtgaagtctt tgatttgaaa cgttcaaatt ataacttagc tcaatatgca
    36001 acagtaaaaa cagtatcaaa tggacaaggt aaatatccag tagcaactaa ccaacaagca
    36061 gtgttagcaa caaaagctga acttgctgaa attggtgata ttgacgctga aatgtttact
    36121 tcagttgatt ataaagtaga aactcgtgct ggtaagattg ccttatcaaa tgaggttgtg
    36181 gaagattcag cagtgaatat tgtacaagag gtcaaagatc agttagcgaa attggtagaa
    36241 aacactgaca ataagcatat catggattta ttaaaaacat tcactaagaa aacggctgct
    36301 acgttggacg atttgaaaca actatacaat gtggcattag acccagcatt aaataaaatg
    36361 gtaattctaa accaaagcgg atattaccac ctagatacat tgaaagattc agatggacgt
    36421 tacattttac aacccgatgt gacagcacct agtggtaaat cattattcgg tatgccagta
    36481 gtattgattg cagatacatt gtttgccaat cctaaagctg gaacattccc tatgattatg
    36541 ggggatattg cacaatctat ctttgttgct cgtagaaatc aagtaacgac tcaatgggaa
    36601 aaattcgatt actactcaca aggacttgca gtgatcgttc gcaacgatta taagaaaatt
    36661 gatcaaaatg cttcagtgta tattgagttt acgccagttg taacaccaaa agtatagaaa
    36721 aatattgggc ggcggtttat cccactgcct ttttttcatt aggagagata aaatggtaat
    36781 tttggatagc ataaaaaaaa gtatgcggat cgatcacact attgatgatg actttattca
    36841 acaattgatt gatacagcag gcgaatatat aaaaagtgct attgatagta gcgcaacgga
    36901 taaagatatg gataattatc agcaatttga tttggcggtg tcattactta ctcaacattg
    36961 gtatttgaac cgtcaagaag ccagcagcga acggatacca gtaacggtac aagcattagt
    37021 acaacaaatg agaggtgctt attatgccaa tcattaagaa tgtaaatgaa ttgactgaga
    37081 gagtcaagtt taaaaagaca aaacgggtaa aagatgaaga tgggcagatg gtggatagtg
    37141 aagaaactgt atttgaatgt tgggctaatg tgcgttcaca aatgctaaaa gacgtgcttg
    37201 ctagtgtagg taccattctt gaaggaacgt tgacgtttat tattcgatac gatcaagatt
    37261 atgaactaac aaacgatatg aaagtagctt ggaaaaacaa gagttataaa attatttcaa
    37321 tcaatgaagg aacggcgttt aaagattata caacgatcat agctaaacag atttcttaga
    37381 atgattacac ttgtaaacgt tgtgggtatt tagtataatt aaggtagtaa atgaagggtt
    37441 agctgcctga attttgcaag acttagctag tcgaaatcta ttgttggact gaaaattgta
    37501 ggtgtggttg caaacatgct ggactaagaa agatgataga tgatgaacgt tcaattttaa
    37561 tacaggcatg ctaggactga ttggatggtt ttggcaagct gattgtgtag aaatcaaaga
    37621 acacgtctgt tgtggacgtg ttttttttgt tataattcaa aagaggtgat ttaatggaca
    37681 aagcagagtt tacaaatttt tattggaagt attatctaca tttagaaaat gagtttatta
    37741 ataccacaga ttttgtgatg ctcgatgaga ataatttcaa aacattctct attgaatatc
    37801 aaaaactact actagcgatt ggttcggagt gtgagattat tttcaaagaa ctttgcggat
    37861 ttgatagcag tagcaataaa agcataactg attttaaagc tgttattaaa tctagcgaat
    37921 taatgtgttt ggataatggt gttagggtta tgaattcttt aacattgact tcactaaaac
    37981 cttttggaga tgggtggcct gaagatacac cagattggtg gaaaatatat aacagagtaa
    38041 aacatggtcg ttcttcaaat tataaggaag caaatttgga aaatgtgttg tatgctttag
    38101 caagtctata tttattagag gaatatttgc ataagaaagt aatactagaa ggtgaagtag
    38161 actttataag tccagagtca aatctattta cgttatcttg ggaaagaaaa cattcgtcga
    38221 tgcaaaaagt gacgtttgaa aaagtggatt caaattatgt agctccactc attgatttta
    38281 aagaacaaga agaaatttaa aaacccccac ggcttattga agtttttgta tgccatattg
    38341 tatgccattt ggtttaaatc gcttgaaaaa tagtgataga taactctaat tcatttagtc
    38401 ataaacgctg ttttgataac ctttatagtt ctttgatatg ggtttctcat tcatggcgga
    38461 agaagaagaa tagaacaaaa gaatgaatta ataagatata aaaaaaggga ttgtgacaaa
    38521 cgtcttgtca caatcccttt ttccaaataa acgatgttca atcagctgcc cctcggcatt
    38581 cgctcgaaaa acggcgaaat atgaagaact attttgccat ttgtttctct aataatcagg
    38641 tcaaaacgtt gacttcacag tctcttcctt aaacggtgtt ctcctgccaa ctcctcggtc
    38701 acaagtcaca atattcaaca aacatgagaa gctgtttttt gaaattgttc cttgtgactc
    38761 ggagctgaac ggcaggctca cgacctcttt cttaaacggt gttcaaaagc ttgcatctgc
    38821 ggtaataagt tcaaccaaac gaaaattatg aggagctatt tttgtttggc tgttcttatt
    38881 acttgatgca ggacagcttt ttcacaacct cttttcgttt agcactaaat ttccttaaaa
    38941 agtagaaaag ctttcgaagc ctcgtttgtt tcttttactg tataataaaa gaaaagagta
    39001 acaatcgcta aaagatataa acaagagaaa ggaaatacaa gatgattggt ctgatttatt
    39061 ttgtggtgat cgttctggcg aacacagttg gcgctatttc cgggatgggt ggaggcgtgt
    39121 tgatcaaacc gattcttgat cttatcggcg cccattctgt tgcagggatt tctttttact
    39181 caacagttgc ggtattcacg atgtccattg tttcgactgt gcgacaagta tcaagcggga
    39241 aatcactgaa ttggcagatt gtcggttggg tatctagtgg agctgttgtt ggaggagtag
    39301 tcggcaacat tgtattcgaa gtttttttac agttatttga aaacgagaag catgtgcagc
    39361 tgatccaaat ttttttgacg gtattgacct tggtttttgc gttcttctac accaagcatc
    39421 atcagccaaa atttcatttg acgtcatgga cttggtacct tatatgtggg gggggttctg
    39481 ggctttttag ctagtttttt aggtattggt ggtggaccag tcaatgtttc tttgttgatg
    39541 ctgatgtttg cattgccaat aaaagaagca acactatatt ctctatctac gattttcttt
    39601 tctcaactgg caaaattggt aacgattgct ttaacaagta gctttatgcg ctttgattta
    39661 tctatgttgt tttatgttat ccccgctgct gttattggag gtttgtgggg agcaagattt
    39721 agcagggttt tgtctccaaa aaaagtgacc tttatttttc aagtaattgt cctcgtggtt
    39781 cttttggtca attgttacaa tgcctttgtc atcttacaaa cattaggaga atctagacta
    39841 acgcaagtca aaatcactga aacatattgt tctaaatgaa aaaaagagta tactatgata
    39901 aaaaaattca ttttaaaaaa tcagaaattg gctggtgatt tggatgttac gggaaatgcg
    39961 tttagaagat gcagaagcac tacgtgaaat caatgcggat caattagggt atgatatacc
    40021 attaaccgtt acggtgagac aaatgaagaa actgctgaag gagccagaga aaaatttttt
    40081 cttggtttat gaagatccta cttctaaaaa agcagctggc tatgtacaag cggagtccta
    40141 tgaatcgatt tttagcgatc caatgttcaa tattatggca cttgctgtgt ctaaatatgc
    40201 tgaaaaaaga ggcatcggaa aggctttgat gactggcgta gaagaagaag cgaaaaaacg
    40261 aggaataacg acaataaggc ttaattctgc agaatacaga gtagaagcac accaatttta
    40321 tgaacacatc ggttatcatt ccgataaaat gcaaaaaaga tttttgaaaa tcctgtaaat
    40381 ctttatcaaa aaaatacccg atcaccaagc cgatacttga tgatcaggta tttttgtttt
    40441 tttgactaag aaagtaattg catcaagaaa ccagagatga tcaaaacgat ggcaccacca
    40501 agtcgattgc ccatttgagc aaaagcaata agttccatcc gattagctgc agataagacg
    40561 gctacatttc ctgttccgcc catcgagttg ttgcataagc cagcagtaat agttgattca
    40621 attggatata aaccaaacaa acgaccgaca aatcctgaaa caactgaaat cgcgataaca
    40681 cttgtcagac ataagacaac aaattgccat gtcaaagcag aagctaatac attcagattc
    40741 agcaacgcaa taccgatacc ggcaagaaca gctgctgtca agtttttgac aatcaagtta
    40801 ttgaacatga tagctgcatc ttcataatac tctggtaata ggttggcgat tttacagatg
    40861 acaacgataa taatcataaa tgcgtaagca tggactttag gaacaaaata gttaccaatt
    40921 tgtcctaaga tgaagaaagt caatgatacc atcaaaccaa cacctaactg tggaatggaa
    40981 ggtttcacag ttcgaggttt tccatcgcca agatcgcctt tttccattaa tttaccattg
    41041 ccgttgaatt ttggcattga ttgtccagca cgagcaacta atgctgcacc gatgatagct
    41101 aagacatttg tcattgctgt cgcaggaatc aggcgggaaa tgatagaaga agcatccgta
    41161 cctaaaacat ttgcataaat cgttgataaa ggaacggctc cggctccaac accaccagcc
    41221 atcgcaggta atgagatgta taaaacggaa tttgcaaaac catttccgat aagcatgccc
    41281 acgagtccta cggcaaaaaa ggagacgacc attgaaataa gtgcaacggg aataaaacga
    41341 acagaagctt tcatcaatag attacggttc ataccaagga tgctaccagt aatcaaggca
    41401 gcaatataaa agtcccaaaa gcccatattg ctgacaaagt tttctgtgga tttcactaca
    41461 tattcaggta agatcccaaa tgtagcaatc gcagcagaag cgaagatcgc aaaaacggaa
    41521 ccaccaccca aataactttt gacgataggg atttttgtcc cgataaaatg aaataaatta
    41581 ccgaaaataa ccaaaactgc gattggtcca atcatattcg tcggtaattt ccctaaagcc
    41641 attgcaatga tcaggacaag gaccatgatc aaataaagtg gcaaacttac gccggtgatt
    41701 tttgtggacc aaaaaccttc ttttttcttt tcctcaactg cagttgcagt tgttgttttt
    41761 tgtgccattt ttacagatcc tttcttattc tgtatcatat gaaaaaagcc aaagaaaagc
    41821 gacaaaagag caggagttgg ctggccgctg ttttgccgca gtcgcttttt tctttttaga
    41881 cgtcatacaa agtttctgta tgttctttta ctataacgat cttaatcttc taaagattta
    41941 tattcaggaa cccatttagc agattcaact gcttctttga tatccgtgat ttcttcacga
    42001 ttcaagtttt gtgcgacaac tgattttgcg acagtttcag caatcgtttg agaaaattca
    42061 gtgatttttg ctactggagg caagatagct gctcctggtt ttgttacatc aacgattccg
    42121 cctaatgctc gactagcttg agaaatgatt tcagcattta ctcgtgtagc tgttgaagca
    42181 atcaaaccaa gtcctaaacc tgggtacatc aaagcattat ttgcttgacc gatttggtaa
    42241 gttacaccat tatattcaac atcagcagca gggatacctg ttccaactaa tgcttttccg
    42301 tccgtccatt ctattaaatc ttttgctttt gcttctgcca attttgttgg attagacaaa
    42361 ggcatgatga tcggacgtgg tgtatgagca gccatttctt tcacgatttc ttcagtgaat
    42421 gctccaggtt gggtagaagt accaatcatg attgtcggat ggatcgcttt tacaactgct
    42481 tctaagtttg tcaattcatc agcattagtg aaatctgagc gtttgcgagc gaatgggatt
    42541 tgaccaggag tcaatccttc tgtatcttca aacaataaac cttgtttatc tacttgatag
    42601 aaatgttggc gtgcttcttc ttcaggtaca ccttgacgga tcatctcatc taaaaggatg
    42661 tttgcgatac ccacaccagc agtaccagca ccaaatgtca aaattatttg atctttcaat
    42721 tgttcaccgg atatattcaa tccgcctaat acacctgcta ggacaacaat tcctgtacct
    42781 tggatatcgt cgttgaaagt agtgatttga tcttcgtatt tttctaagat cgctgcagca
    42841 tttcctcgtc caaaatcttc ccaatgcaat aacaattctg ggaataattc ggtagctgat
    42901 tgaacgaatt gatcgatgaa ttcatagtaa gtttcgcctt ctacacgagc atgacgattg
    42961 cctaagtata agggattatt caataattct tcattattag taccagcgtc gatcgataca
    43021 ggcaaaactt gtgctggatt aataccagca gcagcagtat agaccatcaa tttgccgata
    43081 gcgatgtcaa caccgttcac accccagtcg cccataccta agattccttc tgcatcagtc
    43141 acaacaatca aacgaatgtc tcgtccatct gccgcgttct tcaaacttgc tttgatgttt
    43201 tctggttcgt cgattgaaag aaatgcagca tcttgcggtt tcaagaaaat ttcgttgtat
    43261 tgttcgattg aatccgcaac gacaggatca taaacgacag gcatgaattc aactaaatgt
    43321 tcgcccatca atttataaaa caatgtccga ttcgtattga atagattcat taagaagata
    43381 cgtttttcca aatctgtttg tttgcttaag tattgaccat aagcttgaac agattgttgt
    43441 tccagtgttt ggacagtact tggaagcata ccagtgattc catattttgc ccgttcttct
    43501 tttgtaaaag ctgttccttt atttagaaaa ggattattta ataagtttaa ccctgtaagc
    43561 atgaatgatt cctcctaagt tttaatacaa acgaagtata gaagtcagat gatgatatag
    43621 tcaaaaagag caggtatgat aaattttatt tatatgtaat atagccaaaa gaaatcaaaa
    43681 tagcagtatt tgttcttttt atatcgacaa tccttgcttg aatactatgc gtatctctta
    43741 tatgctagag aaagaaaagt agaagaataa gggatagatg gaatggataa ataggatttc
    43801 tgaggaaaaa agcacatgat agcgttgata ggagtcacct atgtcaggtg ataaaaatac
    43861 ataagtagaa aattaagagg aggaagagaa aatgaaagaa gttcaattga ttagaaaaag
    43921 cgaactatca gaaggcggtt gcaatgcatg tggcgtagta gaagcgacaa gctatacatt
    43981 aaaattagat tccaatcaag caataatctc tgaactgaca gtaggcggtc ttgtggacag
    44041 tctggcgctt gctgaaggtt ttactggaga agatatttat gagatgtttt cagaagtccg
    44101 tcagttaaaa aaaggagaaa aatgcataga ggttcatcat gagagcccga atgtgaggtt
    44161 caaacgtggt gataatgaga tgatcttcaa gaatcatgtg tctgatcaca ctgaactata
    44221 tgaaatcgtg aatcaaatac tgtctgggat ttttgaatta ggaccttatg agttcaagga
    44281 agaaaacggt aatccaaaat taaatgaaga atggcaagaa acgattgaaa cacaaagaaa
    44341 caatccacac ttatttcaat agtcttccac agtatttcgt aaaaatgttg atgaaaaaga
    44401 aacaccgtga atcagaggtc gtgaaaaacc tgccctgcat caagtaataa gaacagccaa
    44461 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagtttt
    44521 tgaacaccgt ttaaggaaga gactgtgaag tcaacgttct gacctgagta ttagagagac
    44581 aaatggcaaa atagttcctc atattttgcc attttttgag ctaataccga gggtcagctg
    44641 attgaacacc gttttatcga gaggttgtga aaaagtcgct tagtttcgag tagtaagaaa
    44701 aattttgtga aaatggcttt ccacattttt gcataaattt gacttaatac cgagaaaatg
    44761 gcttttgaac accgtttatt caaaaaaagg gactttgaca ctacttttgt caaagtccca
    44821 tcggtttttt atctattttt attacaatca tttcttccag agtagtgaca tctggaatgg
    44881 ctgaaatgta aacagattat ttgcctgtat caatgtcgaa gagtaatata aaaagtgtaa
    44941 acgctatcat attgttcatc attcaaattt tatagagaga aggcattgag atggaaaaaa
    45001 catatcaagc aggtacaaat gaaggaatag tagattttat caatatggaa gatttagaaa
    45061 tcgcggcttc acaagtgatt ccagctggtg gttatggtta catcagcagt ggtgctggcg
    45121 acttatttac ttatcaagaa aatgaacggg cattcaatca tcgtttgatt attcctcatg
    45181 tattaagaga tgtagaatta cccgatacaa ctacacattt tgacgaagaa atgctgactg
    45241 caccaatcat catggcacca gttgctgctc atggattagc tcatgtgaag gcggagaaag
    45301 cttctgcaaa aggtgtagca gattttggga cgatctatac agcaagttct tacgcttctt
    45361 gtactttaga agagataaga gaagcaggcg gggagaaagc accgcaatgg tttcaatttt
    45421 atatgagcaa agataatgga atcaatttgg atattcttga agtggccaag cgtaatggag
    45481 caaaagcaat cgtattgaca gcggatgcaa cagttggcgg aaaccgtgaa acagaccgca
    45541 gaaacgggtt taccttcccg ctgccaatgc cgatcgttca ggcttatcaa tcaggagtag
    45601 gccaaacgat ggatgctgta tataaatctt ccaaacaaaa acttagtcca aaagatgtag
    45661 aatttatcgc tgctcattct gatttgccag tctacgtgaa aggtgttcaa tcagaagaag
    45721 atgtctatcg atcattagaa tctggtgctg gtggaatttg ggtatctaac catggtggcc
    45781 gtcaattaga tggggggcct gctgcgtttg attcgttgca atatgtggca gaagcagtag
    45841 ataaacgtgt accaatcgtg tttgacagcg gtgtacgacg tggacaacat gttttcaaag
    45901 caatcgcatc aggtgccgat cttgttgcta ttggcagacc cgttatttat ggattgtctt
    45961 taggaggaag tacaggagtc catcaagtgt ttgatttctt taaaacagaa ttggaaatgg
    46021 tcatgcaatt agctggaaca caaacagtag aagatatcaa aaaaataaaa ttgcgtgaga
    46081 atcgttttat ttaagaaata aatgacgaaa aaaataggag tgcggcagat gatgccgtac
    46141 tcctattttt atagttcaag gatgtcacta atagaataat ttgaaacgaa aaaataaaat
    46201 tgaaatattt tttcatactt aataaaaaag gaagaatatt catgtaaacg attttattgt
    46261 ttttttctct tttatacaaa aagggtcata atttcaagta agcggatgaa ttcatgagga
    46321 ttttttgcat actgtctcta ctatacttag gtcactaaga aaaaggagtg atttattatg
    46381 aaaaagaaca tgattgggac attattactt tcaacacttc ttctaggcgg cttggcaaca
    46441 ccagcttttg ctgagggtca agcgacttca aaaggagata ttacctttac tgaaccaaca
    46501 aataccgttg agcctcttaa ccctactgat ccttcaaaac cagtagagcc agcagatccg
    46561 gaaaatccag caactggcca gacaggttct ttaacattgg atgtagttcc tgaattgcca
    46621 tttggtacac acgagattga aagcggaaca aaaacgtatc aagtagacgc ttcaaaaaac
    46681 gatacacctt atctccaagt atcagatcgt cgaggtgtag gtgcagatgg ccaagcacaa
    46741 gggtggaacg tgacagtttc ggtttcagat tttgtaaacg gttcacaagt tttgcaaggc
    46801 gctgaattag attttggaac atccactgtt aaaagtacgt cagataacga atccactgga
    46861 ccaactagtc aaactgtaac tgggttgagt aaagcaagtg cagcgacacc aatatttaca
    46921 gcagcaaaag atcaaggtct gggaacatgg ctttctgttt atgatccagc aaatatcaca
    46981 ctgaaagtgc cgaaagcagc agctggaaca ttcacggctg atttgacttg gaacctcgtg
    47041 gctggcccag tagcatagag tatgaaataa attcaacaaa ctgcggtgaa aatactatag
    47101 gtgaaggatt ttttaccgca gttgttgttg aaaggatgtg gtggacaatg aatcaaagta
    47161 aacgactgtt tgtttcgttt ttttctattt taagtctatt tttcattttt ccgtcaatca
    47221 gccaagcaaa ggattcagca ggagactttg gaatcaaacc ggtatttcca gagaaccaaa
    47281 tagataaagc aatcggttat tttgatttat tagtggcacc ggaacaaaat cagacactag
    47341 aagtaattat cagcaattct tcagatgaag aacgaacatt tgaggtctct gtcaatccgg
    47401 cggtaacgag tgatggtgga acgatcgatt atagtcaaaa aaacccaacg ttagatgaga
    47461 cattgccgtt tgatgtccgt gacgttttgc tcattgcgaa aaaggaaatc aatgtatcag
    47521 cacatgcaga aacaactata cctatagaag tgaaaatccc agcaaaaagc tttaagggac
    47581 gtgtattagc gggtattcat gtttcaccta aagaagaagc tgaaacagaa aatgctacag
    47641 aaggagcaca aatcaaaaat cgtattgctt ataatctcgc agtcgtttta caagaaagtc
    47701 aagagacgat agaaccggat ctgaaacttt tatcaggaga tttggacgag gtcaacgcaa
    47761 agccaactgt tcagcttcgt ttccaaaatc ctcagcctag gattatcagc aatttgatat
    47821 ttacgagcaa gatcttttat gaaaatcagc tttatattga aaatacgtct aatgaatttt
    47881 tagttgcacc aaattcgaat tttcacttga atcttgattt agctggtgat aaagcaaaag
    47941 ccggcgatta ccgcgcagaa atcatagcaa aatcaggcga cagtaatgaa tggcgattta
    48001 cacaaaactt tacgatcaaa aaagaaaaag cacaaaaagt aaatgaaaat tccgtttttg
    48061 cagtacaaga acaatctttc ccatggcttt atgcaggatt ggttgctgca gttctagtgt
    48121 tatggttgat tttgctgttt ttgtgggtcc acaagaaaaa gaaaaaagga gaaacaacat
    48181 gatgaggtga tttcgttgaa aacatcgatc ttattgggag ggattattgc aagtttgctg
    48241 ttcttcagct ggcaagagcc agtccaagca gataccaaaa gttcaggtac gattacattt
    48301 tatgatctaa gtccagttcc gcatccaagt ggttcagtca atggcgcatc aaaacagcag
    48361 acacagctac cgatttataa agaatctaat caacatggcg tattcccgga gacgaatgaa
    48421 gaaaagatcc gttttcttta tttgatcatt ggtatagaat tatggcttct tgcgtggttg
    48481 ttgttatgga gaagaaagga aaaggaggat gaacatgttg ctacaaaata aattttcatt
    48541 ttttcgctcc ttctttttta cttcgctttt tgttttaagt atatttatct tttcaccttc
    48601 ggcagaagct gttggagcaa cgcctcctgc catttcttat gatccataca cgtatgaaaa
    48661 tatcgtacct ccaaatggtg gacgtagtgc agtcaatggc gggaagcttg tccaactaac
    48721 tggggctaac caattgccta aattgactgc tacttatttg aacgatggtt caaatgatcc
    48781 ttggaaaggt ggcacggtcc gttttcattg ggaaagccat gtgaaactaa caaatgtact
    48841 tgatttggtg aatatcactg cacaaggatt tgcatttgtg gcgatcatca atttgccgga
    48901 acatgtatca agcagtgatg tattaaaagc aattgactgg aacaacgcct acttgtatat
    48961 tgccggtgcc cccatcaagg ttactagcgg tagtttatta tcagcaggaa gccatacctt
    49021 gcgttttggg atggggtcgt gggatacaaa tactctattg gcagatatta ttgctattat
    49081 tacgcgaggc gaattcaacg tggacgatat cccgcttcaa tttgatgtta cagctaatgt
    49141 cgcgaagatg acagaaaacg gagataaata tgatacgagt ccaaacaaaa ttttgaccaa
    49201 gggtaaatta caaccttcta tgtctaaaaa ggccgttttt tctgttgatt tttatgattc
    49261 gaataatatt atccaagatc gtatgttcaa ttcgatttta gctccagctg caggtagatt
    49321 ttatcctaaa ttaactggcg gaaacttgaa tccaggagca tatgcatttt ctgcaagtgc
    49381 agagataaat acgtggaata gttacatctc aacaagtgat tcaacaggaa aatataatac
    49441 atttgctgat accacagaat acgtggatgg aaataaccag aaccttccag gctctaccaa
    49501 tagtcggaca ttgacgatgg ggctgactga aggacgtttt gatcagttgc catttagtcg
    49561 ctttgatcgc gtagtcaact attttacagg aaaaaacgtt acacaaggag caatactgac
    49621 tcatactcca gcaagcacaa gtggattgaa tcagacagcc aatatcgtgt acagtgggaa
    49681 agatgcgcaa ggcggattgc tttctcctgt tgctttaaag gtattggagc agtacaagct
    49741 agacggaacg gttttaccaa caaacttaac agccgatagt cagtggggaa agctcctttc
    49801 ttctgcatcg taccaagtca cggccaattg gtcagcaact gaactaaaaa agggttcgat
    49861 ccattacagg ctttattcga aaggaaccca aaaattagcg acaggatgga cagatcaact
    49921 tttccaaacg atccaatcaa ataacggggg tgtaaataca gctacttcta cactacctgc
    49981 attggcttca gggcaatatt attttgatta tcgcttggtg gacgatgtac ttactaacat
    50041 gtatccgtct tttgcgtaca aatggcagtc tgaacaagct ggaatcacca ctcttcctca
    50101 aataacagtg gctaattttc catctgtgag tgcagatagt cagctaaaaa accttagcag
    50161 acaacctgag acaggagatc ccctgtctgc tctatcagga gatacgattc aagaaaatgt
    50221 gacattcacg ttaacaaaat ctggggatgc gataaccgac aaaaaaatca cgatctcact
    50281 tcctgaaaat actacttata tcaatggttc cttaaaatta aatggtacaa ctatcgcaga
    50341 taccggcatt caacaaggag tttctgtccc agcagatcta ttgagcaaaa taggagatac
    50401 cattcatctt acttacaact atcaattaaa tactgtagat acttctgtac aatccgtcag
    50461 catactgaca aaggcagctg tactttccag caatattaca ttagcagatg gtgctaagct
    50521 tcctaaccca gttgtccaaa catctgctaa aacaattctt gtccccaaac aagaattaac
    50581 attagttaat gtaccagatg attttacatt tggtaacgat ctccccaaac cgttgaagac
    50641 aagttattat gaagcaaaag gcgatttttc ttttgatgtg cgggatactc ggcttccaag
    50701 cacgtctcct tggcaactga cagggacatt gacttcttta tttaagaatg atcaaggtca
    50761 agaattaagc ggaacaaaat tatacttcaa tcattctgga tcaaaacaat tgattcagca
    50821 aggtcaaaac acgttgattt atgagagtga tggaacagct aaaggagagg ttctcgttga
    50881 ctttcccgat accgatggtt tactcttaga ggtaaatagc agtacgaatg ctcaacctgg
    50941 cgcaacgtat caaggcatgg tcacatggga actgacagct gggccaacca gctaagtgat
    51001 tagaatttgt atggatctgt gataactgtc acagatcctc tttttcaagg atgagaagga
    51061 aacttctgct gttaaaatca gaaatgcttg gaggagtgga cttggaactt ttcagtttgc
    51121 ttgaaaaaaa tgaacaacgg caaatcaaac ttttccaact tttattagag gaaaaaggag
    51181 agatttccgt taataacgta cttacccgct tatcagttga tcgggctacc ttgaaagaag
    51241 atctgtttta tttacaagct aacttggaaa atatcaaaca attggaaatt gttatccaag
    51301 aagggaatat tttattaatc agacatggaa atatagcagc agtcgatgtt tattatcatt
    51361 gctacttgcg ctattctaca aaatatcaga tacttcttta tctgttggaa aatggctcat
    51421 ttgatcggca gaaacttgtc atggaactta atatcagttc ggctactttg tctcgcagag
    51481 tgaaggaatt aaatgattct ttaaatgagt ttcatctgaa aataaaaagc gggcggctga
    51541 ccggtccaga aagtcaaatt tgttattttt atttccaatt attttggttt ggacgtcctt
    51601 atcttgaaaa caaaaaagaa tacagtgatt tatcaggaaa cactttttta gatctgctgc
    51661 aaaaagagtt tagttttact attacagaag aaggacgatt gaaatttgaa atatggcttt
    51721 ccattatgaa gaagcgaatg cgctgtacga aaaaatccaa accagattat agcaatcttc
    51781 ctgttggatt gtttcaaacg acacaatttt tattggacct ccatacattt ttgacccgtt
    51841 acttctttca atatgccttt ccttggaatc aaaatgaaac gttggttttt tatcttttca
    51901 tgatcagtaa ctttacatta gatacacata actcgcatgt ccaggagttt ttgataagcg
    51961 aaaaatacaa agacgacgta gtagctcaat tgaatcgaac gtttaaacaa tcgatagaag
    52021 aatatttctc tttttatgtt tttccagaag agatttcgca aaagattgcg ttgacagtga
    52081 tgcaaaatca cgctcatttt gcctatttta gaggatggat gaatattttt ggccaagaag
    52141 gattaaaaca gcggttaact gaagtagaag ataaacaatt cctggtttta tgtgaaagat
    52201 tggtcagaca aacatttgct attgttgatt ttactgaagt agacgaggaa aatgcccgct
    52261 ttgaattatt tgctcgctat gcatctattt tgcggacgct gatcaagcag ttagaattac
    52321 gtctgcaaat tgcttatgat cttccttatg aaaggctaat ggcggatatc gtgaaagaaa
    52381 tcatccagga acaattgctg aatacgatca aatatgattt agtcgaatac gagaaagaaa
    52441 agcagtatga tatcattttg actagccaat tgaaagagta cccgtcacag aagtccgcta
    52501 aagtttttgt attcactagc aacgaatata attatgattt cccctatttg aaccaatttt
    52561 taaaagaatg ttatttagaa aaattgaatt taaggatgaa taaaacatga aaatcgtgat
    52621 cattggtgct tcccatgcag gcatcacagc tgccctaaat ctaagaaaac ttcaaccgaa
    52681 agcagaagtt ttactgatcg atgaagatca taaagatgga ctaggctatg tttcaaatgg
    52741 aatcaacctt tatttaaaag gaaagatacg tagtttagct gaagttgccc ataacatgcg
    52801 gactcttcaa gaaagtgggg caaaactgat caccgaatgg agagtaactg aacttgatcc
    52861 agacaaacat cagctcatcc tcgcatccaa agaaggaaaa aacgagacca taacctatga
    52921 taaattaatc gtagcaacag gttcctctcc tgtcaccttg tataaacaaa ttgaagctga
    52981 aaatgtctac acctataaaa atctagtgca atctaagcaa gtacttgccg ctttaaaaga
    53041 agcaaaagaa gtagtgatct tcggtgctgg ttatattgga ttggaattag cagatgcttt
    53101 gagaaataaa ggatacatta ttcatctagt tgattatatg ccgaatgttt tatcaagata
    53161 tttcgataag gatatgatca acagttttca aaatcagctg caaactaagc aaatcaattt
    53221 ttatccaaat gaattcttga tcgattggaa aaaatcagaa gagaaagtag tatccgtaca
    53281 acttctatct caagcaataa aggcagatat ggtcattttt tctgcccaaa ctcgtcctaa
    53341 tacgacattg ttaaaagaaa aagtggcttt gtatgaggat gagactgtga tagtcaatga
    53401 atatctgcag acaagtgatc cggacatcta tgcgattgga gatatcgtcc cagtcagttt
    53461 tgacaaaaat aaacggcatc tctttttacc tttagttact cgagctgtac atatggcaag
    53521 agcagtagcg ctaactttga gtggtcagcc aaccgcttac gacctcagac aaaagataac
    53581 agcagcagtc attatagact actttttagg aactgtaggg ctgacagaag atgaagcgcc
    53641 attcttggaa caaaataccg gttcgtgttc aggagaattt gatctatttc cacaatacga
    53701 tgaagaaaac aaaacagtaa atgcaaagct gatctaccat ccggatacat tagaaataat
    53761 tggcggacag ctgataagcc aagaattctt attatcagat ttgaacctgc ttgcggatat
    53821 cgtgaaacac aaaactacga ttccacagct agcagttgag gattttggat ttctgtctga
    53881 acacacctct cgttttcact acctcaatga gttagctttt aaagtactag ctaagagagc
    53941 aaatagcaat agagtagata aaagactgtg agaaatatca cggtctttta ttgtgtgggg
    54001 atataataaa tattaatcaa tcgagaataa aaaattcata taatgaaata gcggaagaat
    54061 tttttgagca taatattcgc caaaaattta tcaagtgata aaattgttgt ataaaatgat
    54121 tatttcagga ggaggagtta aatgttttta gcattaaatg aaattatgca ttcaaagtta
    54181 cgttatgcgt tagttgcagg ggtgatgttt ttgatcgcct acctggtatt ttttttgact
    54241 gggttagctt atggcttagc tcaagataat cgaacagcag tcgacaagtg ggaggcagat
    54301 tcgattgtgc tttcgaaaga tgcaaattca aatctaggga tgtccatgat tactaagaag
    54361 atagcagaag aagtcgaagg tgggaaagtg gcgtatcttg cccagacacc tggagtcgtg
    54421 actagtaaag acagtacaga agaaggaaaa atcaatgtta gcttcttcgg tatcgataaa
    54481 aatcaattca tcatgcccaa tcttgttgaa ggaaaagctt ttgataacga tgatgaggca
    54541 gtaggcgata ttagtttgaa agaagaatat ggtctagccg taggagatac agttaagtta
    54601 tctggtagtg acaagacgtt taaactaact ggctttacgg atcatgccaa attcaatgtc
    54661 tcaccagttc tctatacaac catcaatgct taccaagaga tccgcttcga aaaagaagat
    54721 acaagtgaaa atgcacggat taacgcaata gttgtcagag gtaaaatcag taatttacct
    54781 gaagatttag aacagatcaa aatttctaaa ttcatcaatg aattacctgg atataatgcg
    54841 caagtattga cttttggatt tatgatcgga tttttgatcg ttattgcggc tattgtgatc
    54901 gggattttca tttatgtact gacgatgcaa aagattaata tctttggtgt aatgaaagca
    54961 caagggatca caggaggatt tattgcgcgt tctgtagtgg cacaaacatt cattttatca
    55021 tttgttggta ttcttcttgg tctattagga acagtgggaa catcccttgt tctacctgat
    55081 gcggttccat tccaaagcaa ttggctgttt tttggcgtca ttagcttgtt gatgctggta
    55141 gtagctgtgt taggcgcact attttctgtt cggacaatcg taaaaatcga tccgttgaag
    55201 gcaattggat aagaaggagg aagagcatga aagcaataga atttaaagaa gtagaaaaaa
    55261 gtttccaaga tggcgaccaa gtagttaaag cattgaaaga aacaaccatg agtatcgata
    55321 aaggggaatt tgttgcaatc atcggtcctt ctggttcagg taaaagtact tttttgactt
    55381 tggcaggcgg attgcagacg cctagcagtg gctctgtatt tatcaatgat caagaattca
    55441 gtaatcaaaa agaaagtgaa cgcgttaaac tacgttttaa agaaattggc tttattttgc
    55501 aagcttccaa tctggttcct tttctgacag taaaaaagca gttattatta gtagataaga
    55561 tcaaacatga acatcgtcaa aaagaagcaa aagaattatt tgagcagctt ggcgtggata
    55621 agttgatcaa caagtatcca gaagaactat ctggtggaga acggcaaagg gtcgcaatcg
    55681 ctcgtgcatt gtacaatgat ccgtccatta ttctagcaga tgaaccaact gctagtttgg
    55741 attcagaaaa ggcacgagaa gtagtcgata tcttggcaaa agaatcaaaa gacaaaaata
    55801 aagcaacgat catggttact cacgatacac ggctgattga caaatgcgat agaatattta
    55861 tgatggaaga tggtgtattg aaagaaaaag aataaccaaa aagcaggtgg aaaaatggtc
    55921 catcgataca acaaagatac acagcaaaaa attttgaaag aagcgactga cttgtttatg
    55981 tccaagggat atctcggcac ttcaacaaga gagatcgcac aaaaagttgg gatcacccaa
    56041 cccaatctct atcattattt cggtgataaa gaaaaactat atacggcagt tttagaaaac
    56101 cacttaaaag atgttggaaa agctttgaga gaaatcgttc aaacaagcga aacgggattt
    56161 caagtaacct tgacaaaaat ggcgcagttt ttgattgaga cacatctggt cgatttgttt
    56221 ctgatgcttc acgatcttga aagcaatctt tcaaaggaaa caagagatca tcttttcttt
    56281 ctatggaaga aaaattatcg tgaacctttt gaagaaatct tttcagcaaa ccaatctgtg
    56341 ctaagaaatg ggatttcaaa agaaatcgca gcaaggcatt ttttcttagt gttagcacct
    56401 tacatcactc ggtcgaaaag acctgtggaa gaaccgctaa atgtggcgca attgatcgat
    56461 ttatatttga atggtgtatt aggagaagcg taaagctgtt atatagggag aaaaatagtt
    56521 aggtgaagaa gcaagatatg acgaagatcg ttatatcttg ttttttttat ataggtagtt
    56581 tttcataaat acaaatttgt tattctctca tgattatcag ggtatatttt tatttattat
    56641 gtcggtgttc caggaagtgt tttaatttaa taacgaagaa attaattgtt tgtagaatga
    56701 aggaaactat ttattgggta gatgaatcac cacgaatagt caaaaggaga tttcaggata
    56761 tgccaaaaat gaaaaaaatc attttagcag taatgttatt ttctacggtt atatctttta
    56821 cagccccttt tttgcgtact taagctgatt ttaagttttg acccttagtc tggatctacg
    56881 aggtgagagt caagaatgaa aaaaataatt ggagcttttg tatttgcaat cattatcatt
    56941 attatctgtt ttcaaataga tgtttttggt caaaaaaaga tagttggaca aacggtcagc
    57001 agtacgaaca gtacggttac aaagacaaag gaatcatttg atgatacaga acaagagaca
    57061 ttaatcaaaa tcggaaaaaa tgaccgtaca ctatatacag atgactcgct aactacccca
    57121 cttgcaacta taaacggcgg agagctggcg gagtttcttt ctgaaacaaa aaatgcttat
    57181 cagatcaaga caaatgatgg ctatactgga tatttagccc tcgaagatgg aaccaaagtc
    57241 acaaagaata tacaaacaaa accggaagag ctatctgatg cagtgattgt attagatcct
    57301 gggcatggcg gaaatgatac tggggcatta agcaatgatg aacaaacaga agaaaaagat
    57361 ataacattaa gtacagccat aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt
    57421 tatttgaccc atactacaga tgaattagta cagcttggag atatatgcga ctacagtgaa
    57481 gaaaaaaagg cagatgtctt tatcagttta cacgcagact ctacggaata tgcgaatgaa
    57541 gcaactggaa tcacgacata ttattactat ggacaagagg aaacattagc acagacaatt
    57601 gctgactcct tcactgatct gcctctggat tcaagaggaa tctccactgg gaactatcaa
    57661 gttcttcgag aaaatcttca accttctatt ttaatcgaga tgggttatat gaataatgat
    57721 agtgatttaa aggaattagt aacagacagt tatcagcagc agattgctgc aagtttgaca
    57781 caagcattga ccacttattt tcagtaataa ataagcccag ccaaacaaaa atagctcctc
    57841 atatttttcg tttggccggg cttattatcg cagatgcaag cttttgaaca ccgtttaagg
    57901 aagaggttgt gagcctgccg tttaactccg cgtcacaagg agcaatttca aaaaacagct
    57961 tctcatgttt gatgactatt gtgacttgtg accgaggagt tggcatgaga acaccgttta
    58021 ttcggaacaa gggactgtga catgactttt gtcacagttc cattattttt ttagcagctt
    58081 caaagctttt tctgtattgg caaaatgaag tttggcatat ttttcaagca taggcatgcc
    58141 gcctttctcc aatagttttg ccgctacttg gtcagatttc gttccagctc ctgatggaag
    58201 ggtgattcca gcttcagctg atgctttgtg taactcttcc aaaaagctgg cacgactgat
    58261 gatagaagca gcagcaacag ctaaatgata ttgttctcct ttagtaacga agtacagttt
    58321 ttcttgtaat tgattttttt ctaaacgcgc atatttttta aaatttgctt ccggcgtaaa
    58381 ctgatcgatc aaaatcgctt ctggtttttc tggctcgatt ttttttagca gcaaatggat
    58441 cgcttgattg tgcaaagcga ctttcatccg aacggcattg tattttggtt ggatctcatt
    58501 gtatttttct ggagataaga caagcagttt ataggggatg gtttttttta agatgtcagc
    58561 aagctggatg atttggctat cttttaactc ttttgaatct ctaacaccaa gtttacgtaa
    58621 ttctgcaagc tgatcacgtt tcacataagc agcacatact gttagcggac caaaatagct
    58681 gccatttccg acctcatcac tacctaaaac ggataattga ctgatatttt ttggcaaaga
    58741 agaggctgtt tttgctggct tggcttgttt atcctgtgcc tccccccatt tatcagcttc
    58801 tattgctgca ttcgtacctt gaaacatcac ttttccggat gtataagcgg taatggtcgt
    58861 cccgtctttt tttgcagcaa agctagtata aggaatctgt ttatttaaca aataagagcg
    58921 atagtatgct ttcatttgac taagttggtc gtttgttgct ttgataatga cgttgctcat
    58981 aaaattctcc tcgtttttat acataaaact ataccgcaac tcacgacttt tcgttttcta
    59041 agagattgcg agacatagca tttttttaaa gtttatcata taatagttaa gtatgtgaaa
    59101 aatatttgtg gagaacggtg aaagaaccaa cttcatggta cactaaataa aactttcatc
    59161 aaataggagg aaatgattcg tatggcgcac gaaaaaacac gctacaaagc agtgattgcc
    59221 aaccaaacat atactattat cggacgggaa accaagcatc atatggatat cgtaacgaag
    59281 ctgatcaatg aacagttagc agaattgaaa caattatcac cacaaatgga taatgaacaa
    59341 gcagcaatct tgatggctgt caatgcgctt tcggatcaat tgaagaaaca agaaagaatc
    59401 ttggagctgg aagaagaaac agcagaatta aagaaaaaaa tgatcaaatt tacagagctt
    59461 gaaaatcgtg tgaaacgcat tgaagcaatc gagaacgaag ctcgggaagt attgaaagaa
    59521 aatggtcaag cagatcatac cattaaaaat catgtggaag cacaacagat tttaaacgag
    59581 aaacgaaaag gacaaattaa acaaaaatct tctaattaag atccttttat caaaataaga
    59641 aaaatttaaa aaagttagaa gagaaaggaa cgtagcatgc tgagtttgct gattttattt
    59701 ttattgctaa ttgcgttttt ttctggagca cgtcgcgggt ttgctttgca aatcatattc
    59761 gcgattggat atgtattgtc atttattgct gcccagcatt tttacaaacc attagcaagt
    59821 catttggaat tatacattcc atacccagct gtcaccccaa catcaaagtt agcatttttt
    59881 gatcaggtat ttgcttttca cctagatgaa gctttttatg caggaactgc ttttctgatt
    59941 attttgctaa ttggctggct gcttacccga tttgtggggg tttttgtcca tggattgacg
    60001 tatgtgccga tcttgagaca ggtagactgg attgcaggag gcatcctgtc actaatcatg
    60061 gcttacgtta ctatattttt gattttgcag ttacttgcct ttgttccatt agtgtggtcc
    60121 aaaatcaatt cggcgagcat agtttcgctc gttttatcgt tgagagaacg ccatttctta
    60181 ccaacaagat gtatgagctt tgggtaacga atattttgaa ttaaggaaaa tagtgctgga
    60241 aaatatttcg ttttttgtac agagtagttt atttaagaag gctgcgtagc gtatgtccgg
    60301 cacagctctt ttttcatagt gaaagataag gtgataaaat gaataaccga atccttgaaa
    60361 cattggaatt tgaaaaagtc aaacagatgg tcagacaatt tgtagtgact gcacaaggaa
    60421 aagaagaact ggcagaattg gtaccagtga gtgagaaaca aacgatcaca aactggcttc
    60481 aagaaactga ggatggattg aaagtgcaac gtctgcgagg cgggatccct atacctaaac
    60541 ttgaaaatat ccgtccccat atgaaacgga tcgagatcgg agcagacttg aatggagtgg
    60601 agcttgcaca agtttccaga gtattgtcta cgacaagcga attgaatcgt ttcattgatg
    60661 atctttctga cagtgaaatc gaatttgcac gtttgtatat gtgggcagat cagttagtca
    60721 cgatccctgt tttgagccgc cgtttaaagg aagcaatcga tgaagacgga cgcgtgactg
    60781 atgacgcttc tcctgagtta aagagcataa gacagaacat tcggcgaagt gaacaagctg
    60841 ttcgtgaaca gttagatggg atcgtccgtg ggaaaaatgc aaagtattta agtgatgcga
    60901 tcatcaccat gcgaaatgac cgctacgtta tcccagtcaa acaagaatac cgcggtgtat
    60961 ttggcggtgt agtccacgat caaagtgctt caggacagac tttatttatt gaaccaaaac
    61021 aagtggtgga tttgaataat cgtttgcgtc aataccaaat cgctgaacga aatgagattc
    61081 aacgtatttt aagtgaactt tctgctgaac ttgttcccca tcgtcaagaa atcatccaca
    61141 atgcttatgt gattggtaaa atggatctga tgaatgcaaa agcacgattt ggtaaagaag
    61201 taaaagccat cgtgccggga atcagtgaag acaatcatgt cgtcttgaaa caagcaagac
    61261 atccactgat cgatcaggaa aaagtagttt ctaatgacat cacaatcgga aaagactatc
    61321 aagccatcgt cattacagga cctaacacag gtgggaaaac gatcacgttg aagactttag
    61381 gtctgttaca actgatgggg caagcaggat taccgattcc tgcaggtgaa gaaagtcaaa
    61441 tcggtatttt tgaggaagta tttgcggata taggagacga acagtcgatt gagcaaagtc
    61501 tatcgacctt ttcttctcat atgacgaaca ctgtagatat tttatcaaaa gtcaatgaaa
    61561 aaagtctggt attatttgac gagctcggtg caggaacaga tccgcaagaa ggtgctgctc
    61621 tagcaatcgc aatcttagat gatcttggta aaaagtctgc ttatgtgatg gcaacaacgc
    61681 attatcctga attgaaagtt tatggataca atcgggcaaa tacgattaat gccagcatgg
    61741 agttcgatgt ggatacacta agtccaacgt atcgtttgct gattggcgtt cctggacgaa
    61801 gcaatgcgtt tgaaatctct agccgattag gtcttgatac agaagttatt gatgaagcga
    61861 agcagttgat gaatgatgag agccaagatt taaacgaaat gatcacagat ttagagaatc
    61921 gccggaaaat ggcagagacc gaatatctgg aaatgcgtca ttttgtttct gaggcacaag
    61981 aacttcacga tgatttgaaa gaagcttaca gctatttctt tgaagaacgt gaaaaagaaa
    62041 tggaaaaagc aaagaaaaaa gcaaacgaag tcgtgtcaga ggcggaagaa aaagcagaaa
    62101 aaatcattgc tgatattcgt aaaatgcaac agcagattgg gcaaggaaat gtgaaagagc
    62161 atcagttgat cgatgcaaag acccaattag caaatctaca tcaagaagaa actttgaaga
    62221 aaaataaagt attgaagaag gcgaaagaac aaaaaacgtt gaaaccggga gacgaagtgt
    62281 tggttacgac ttacggccaa agagggacgc ttttacgtaa aaacgggaat cagtggcaag
    62341 tagaaatcgg tatattaaaa atgaatgtgt ccgaagacga gctgacgcct gttgctcctc
    62401 aaaaagagcc gacacaacga gttatccatg ctgtacgatc tgaatctagt agtcacgtgc
    62461 cgaatcaatt ggaccttcga ggaaaacgat acgaagaagc gctaagtgag gtagatcaat
    62521 atttggattc tgccattcta gctggttatc ctcaagtgac tattgtccac ggaaaaggaa
    62581 caggcgcact tcgcaaggga attactgatt atcttaaaaa ccatcggagc gtcaaaagct
    62641 ttgagtttgc accggcaaac caaggcggaa atggtgcgac aattgttaaa tttaagtaaa
    62701 gcttactaaa ggtaagcaga tggttcgcta tccaagtata gaaatgctat actaatcata
    62761 gattttaaaa atggaggtct ttgatatgtc acaagtaatt acagataaga catttaatga
    62821 agaaacagat aaaggacttg tattgattga tttttgggca acttggtgtg gtccatgtcg
    62881 tatgcaagca ccgattttgg atcagttgga acaagaatat gatgaagaag aattcagaat
    62941 tgctaaaatg gatgtggatg aaaatccaga aactcctcaa caattcggta tcatgagcat
    63001 tccaacatta atgttgaaaa aagatggtca agtagtagaa aaagctgttg gtgttcattc
    63061 aaaagaacaa ctacgtcaaa tgatcgatca atatttataa aaacttacag caaaaataag
    63121 ctgagggtgg gactgaatcc agttccgtcc tcggtttttt tgcggttgag ttaaggttat
    63181 tcaaattaat attttttgag tataattacg ttgtcaggct ggattgtttt tttgacagac
    63241 atgctataat aactttaatt gaatatgaag gggagtacca gatgaacgaa cgaatcaaaa
    63301 acaaattagc tttacttcct gatcagcccg gctgttactt gatgaaagat aaaaatggaa
    63361 cgatcattta tgtcggcaaa gccaaaatat taaaaaatcg tgtccgctca tattttactg
    63421 gcagccataa tacgaaaacg gaacggcttg ttagtgaaat cgaagacttt gaatacatcg
    63481 ttacagaatc gaatatcgaa gcattgctct tagagatcaa tctgataaag aaaaatgatc
    63541 ctaaatacaa cattatgtta aaggatgata aaacctatcc tttccttaag ataacgaatg
    63601 aaaaatatcc ccgtttggtg attactcgta aagtactaaa agacaaagca ctgtattttg
    63661 gaccatatcc cgatgtcggt gcggcgaatg agacaaagaa gatcctcgat cgattatttc
    63721 ctctgagaaa atgcaaacct agtcagacga aggagccttg tctttactat catttgggac
    63781 aatgtctgtg tccttattat ttcgacgtag atccgaaagt ctatacgaaa atcgttgatg
    63841 aagtgaaaaa gtttttaaac ggaggacacg aaaaaatcga gtcagagatc catcagaaaa
    63901 tggaaaaagc tgctgaaaac atggagtttg aaaaagctgc agaataccga gaccagatcc
    63961 gtgccattga gacaatcatg actcgccaaa aaatgacaaa tactgattta ctggatcgag
    64021 atgtatttgg ttatgcgatt gacaaaggat ggatgtgtgt ccaagtgttt ttcgtaagac
    64081 aaggaaagct aattgaacga gatgtttcaa tgtttccttt ctataacgaa gcagaagaag
    64141 atttcttgac ttacctcggt cagttttatc aggaaaatga gcatttcatt cctaaagaag
    64201 tcttgattcc tgatacgatc gacaagccca gtgtagaagc tttgttagct acgaaagtgc
    64261 tgcaaccaaa acgaggagaa aagaaaaaat tagttgaatt ggcgaataag aatgcctcag
    64321 ttgctttgaa tgaacggttt gatctgattg ccagaaaaca ggaacggacg atcggagcag
    64381 ttgaacgttt aggaaacgcg atgaatatcc cgaccccaat acgtatcgag tcttttgata
    64441 actctaatat catggggacg aatcctgtgg ctgcgatggt cgtattcatt gatggaaaac
    64501 cggcaagaaa agaatatcgg aaatataaaa taaaaacagt caacggaccg gatgattatg
    64561 catcaatgag agaagtcatc tatcgcagat attcacgagt gattcgcgaa gggttgcctt
    64621 tacctgattt gatcttgatc gatggaggaa aaggacaagt agatgtggca aaagatgtct
    64681 tagaaaatca attaggaatc gatattccta ttgctggcat ggcaaaaaac gataaacata
    64741 aaacaaatga gttgctattc ggtatagatt tggctgtagt accgcttgag cgaaattcac
    64801 cagagttttt cttactccag cgaatccaag atgaagtcca tcgattcgca atcactttcc
    64861 atcggcagct acgcagtaaa aacagttttg cttcccgatt agatgaaatt gaaaggttag
    64921 gaccaaaacg aaaaaaaatg ctgcttaaag agttcaaatc gttgaaaaat atcacagcag
    64981 ccacgacaga agaattacaa gaaatcggac tgccgaaaaa tgtcgcacaa aacgtatttg
    65041 ataaattaca tcaggaatga ggaaagggca ctttacaaag aaagtaaagt gtcctatcat
    65101 tttatgtaca acaaaaagag cgtttacgtt ttgttggtaa ataataaatc gcttacgtct
    65161 ggtgtctaag gatatatgtg atttcttgaa gaaggagtga agaaatgtta gaagtcaagg
    65221 atcttgttaa aacttttggg aactacacag ctgtcgatca tgtctctttc caaatccccg
    65281 atggaaaaat catggggcta attggccaaa acggggctgg gaagacaacg acttttcgtt
    65341 tgatcttgga ttttttgaca caagaccaag gagaagtgtt atggaatggc catcatcttg
    65401 gggagaaaga ttatgatgtc atcggttatc ttccggagga acggggactt tatccgaaag
    65461 taacgattca agaccaactg ctttattttg cagaacttag agggaaatca agaaaagaga
    65521 ttgaaccaaa aatcgatttt tggatggaaa aatttcaggt aaaagggaaa aagacggata
    65581 aagtaaaatc tttgtctaaa ggaaatcagc aaaaagtcca gctgatcgcc acgttaatcc
    65641 atgaacctaa attgatcatt ttggatgagc cattcagcgg gttagatccg gtaaatgctg
    65701 aattgctaaa agatggaata ttggaattga aaaaacatgg ttcatgtgtc attttttcta
    65761 gccacaatat ggataatgta gaaaaaatct gcgatcattt gatcatgttg cgaaacggaa
    65821 aaatggtttt gaatggaaaa gtacatgaga ttcgtgaatc atttggccgt acgaagttat
    65881 tcttggaatc tggtttaagc caacaggaag tagaagaaat cgaaggcgtg aaaaaagtga
    65941 tccttcatga agacaatacg ttagagatta ccttagaaga tcctgaagct gggaaagaaa
    66001 tctttactcg tgcgactcag ttcggctaca ttcctatgtt taaccagcaa ccgccaactt
    66061 tggaagagat tttcaaattg aaagcaggtg aaatcgatgc gtaagttttg gattattacc
    66121 aaagatgtct atttaaaaaa tgttaagtcg atttctttct tgatcatgat attagtccct
    66181 tttatcatgc tgggtatcat ttatgtagca ggaaactttg ctcaacaaaa tagtgatgtc
    66241 aataaaatcg gcgtattggc tgataatgag caaatcagtc agcaaatgag ccaagtcaaa
    66301 acagatgatt ttgagttcaa agcagttgat tctgaagaaa aagcacaaaa agagctttcg
    66361 gaagaaaaaa tcgatgcgta tttagtagtc tcaactgaga atgataagat aaatgggatg
    66421 ctttatagcg aaaattcatt aggacaaagt acacaactac tgatacaaca acaactgact
    66481 ggcttgcaat cgatgcttcg agcaagtcaa ttaggcattt caccagaaga agtagctagc
    66541 ttgagccaac aagcaagttt gactcgacaa aaagtcaatt ttgattcaaa aggaaaaatg
    66601 acagttggag aagataactc gacagtccaa tatgtcgtaa gttatgtagc aacgattgtc
    66661 ttgttcatta ttattttgac gtatgcgcag atcattgcac aagagatcgc ttctgaaaaa
    66721 ggcacgcgga tcatggaagt gatcctgtcc agtacgactg cacaaaagca tttttacgga
    66781 aaactaacag gtgttttact tgttgcatta acgcaaatgg cattatacgg tattattttt
    66841 ggtataggat acagtcaatt caaagatatg gaaatggtca aaaatatatt agacggtatc
    66901 tcattggata acttgattgg accattcttg tggtattctc ttctattcat gatctttggc
    66961 attttgattt tttctgtttt agcagcttta tgtggctctt tggtcaacaa agcagaagat
    67021 acagcaaaag caattttgcc agtgacttat ctatctctag gcggatatat gcttggattg
    67081 atactaggag cttcagatcc aaacaatatc gttattcgga tcacttcgta tattccattc
    67141 ttatcttcat atattatgcc ggttcgctta gcaaatgaga cagtcggtat gggtggagca
    67201 ttgatttcct tgttggtact tgtagccgta acattcttat tgatgatctt gtctgcaaac
    67261 atgtacaaat cgaatgtact cgtgtatagc gaaggcggat tatggagttc attgaaacaa
    67321 tcgatttcaa ttatgagaaa tgaacgaaaa aaagcataaa atatagaaaa atatagaagg
    67381 aagctggaca tttattgttc ccagcttctt ttttgtatag acctatttct atttttttat
    67441 tatattggat atatatattc attgaaaata gagcataaca actttataat gttgagtata
    67501 ccaatttttg gaggcgtttt aatggaccgc tatttagatt tactttcaga gaagttccct
    67561 acaacggaag atgtcatcac ggaaatcatc aatctcgaag caattttgca gcttccaaaa
    67621 ggaacagagt tatttttgag cgatatacat ggcgaatttc ctgcttttga tcatattctg
    67681 cgtatcggtt caggaaatct taaagaaaag atcaaagacc ttttctctga acaaatgacg
    67741 gaaacggaga tcagccaatt cactattttt acggcttatc cagaatatgt gttgacgact
    67801 gattggtaca aaaaacaaga taaatcccag ctgatccatc gtttgatcga tcttttacgg
    67861 tttacgacag tcaaatacac tcgatcaaaa gttcgaaaag gattgccgaa agaatatagc
    67921 tatatcattg aggaactctt gtatatcgat gatcgtatca acgggaaaaa agactatgta
    67981 aaaaaaatca ttgaacaact tgtcttgatg aaggaagaag aaaaattttt aacaaaactt
    68041 gcgcagacga tccaaaaatc tgtcatcgat catttgcata tcgtgggtga tatttttgat
    68101 cgaggaactc aagcagccaa agtaatggat cgaatcatgg atttttcctc tgtagatatc
    68161 cagtggggga atcatgatat tttgtggatt ggtgcgtatt gcggctctga ggcatgttta
    68221 ttgacgcttt tacggattgc cgcacgctac aactatttat ttgaattaga gaaagaatat
    68281 ggattaaatt tacgtccatt attttctttt gcccatcaaa cctatcagaa gaatccagta
    68341 tttaccccta aaaacaggaa agatctatct gaaagagagc aagaagaatt agaaaagatc
    68401 catcaagcac tttctatcct ccaattcaaa tcagaacatc agttgttgaa tcgacgacct
    68461 gaatttaaaa tggacgatcg taaattgttg gaaaaaatca actatgagga atcaacgatc
    68521 gttttagaag gtcagctcta tgagttgaaa gggacttgtt ttcaaacgat ccagccagat
    68581 gatcctaaaa aattcttgga agaggaacaa aaagtgatcg atagtttgat gtattcattc
    68641 cagcactcgt taagaatgca aaaacacatg acgtttttga tcgaaaaagg gggaatgtat
    68701 ctcacgaaca atcagcatgt actgttccat ggctgtatac ctgtagatga aaaggggcag
    68761 ctcttagcat ttgaattaga ccaatcttat cgcggaaaag aactgctggg attttttgaa
    68821 aaacagataa ctgaaagcgc aaaagatttg actgcaaatg aagatttggc gaccgatttg
    68881 atttggtatg cgtggagcgg gccattttcg cctttattcg gcaagagtaa aatggcaact
    68941 tttgagcgct attttctcac agagcctcat acgcacgtcg aaaaaagcaa tccctattat
    69001 cacttgcgag atgaagagtg gttcagtgaa aaaattttgg cagaattcga tgcaaaagaa
    69061 gaaggttcag caatcatcaa tggacacacg ccagtaaaag taaaaaaagg agagtcaccg
    69121 atcaaagcaa acggaaaagc atttgttatt gacggaggtc tatccaaagc ttatcaaaaa
    69181 acaaccggga ttgctggcta ctcattacta tgcaattctt acggtttcca aatcgtgaca
    69241 cattatccgt tcttgcctat tgaacaacaa tttgcaaaaa aatctgatca aacgaatgta
    69301 aaaaaagtga tcgagcagca gctcccacgc aaacttaatc ggaatacgac aaaaggaatg
    69361 gaattacagc aacagattac tcaactccaa aggctgttgg attatcgaaa ggacgattga
    69421 gaaaaatgct agccaacatt gctcaataga ctaaaaagcg actgcaacaa aaggcgtgtc
    69481 gcagtctcct tcggaattag ctcaaaaaac ggtaaaatat tagaagctat ttttctgttt
    69541 atttctctaa tactcaggtc agggcgctga tttcacgacc tcttccttaa acggtgttct
    69601 cctgccaact cctcggtcac aagtcacaat atccaacaaa catgagaagc tgttttttga
    69661 aattgctcct tgtgactcag agttgaacgg caggctcacg acctaatcag aaaaacgatt
    69721 aaaattcatt cacaaagaaa tcgaataaac gctgttcaga tgaatctaca cgatgagaaa
    69781 gttccggatg ccattggaca ccaaggattt tgtgcatatt atctttactt tggactgctt
    69841 caatgagtcc atcgttggaa aaggcgatgg cttttaacaa aggagaaaga tctttgactg
    69901 cctgatgatg ataggaattc acaaagtatg aatcagggag aaggtcactt aataaactgt
    69961 ccgaaacgat tttgacttcg tgagttgcaa actgcggggc agtgggctgt tgttcgtgtt
    70021 ttacggacca ttttggatat aatgaaagat cttgataaag tgttccctcc aacacgacat
    70081 tcaatagttg aagtcctcgg cagactgcaa aaatcggttt tctttgtttc aatgcttcca
    70141 agatcaatgc ctgttcgaat tggtctcgtt gaatatttgt ttccgtcagt tttggatgag
    70201 ggtcttccat gtaaagttgc ggactaacat cctgaccgcc tgcaagaatg agtttgtcga
    70261 ttttctcaat tagttgaggc gctgaatctg gagacatgac tggcagaata agaggtaatc
    70321 cttgagcatc taaaactgca tccacaaatc cctgcggtgt atagcttact tgatttcctt
    70381 ggaaagtatc tgtcgctcga atcagttgat tcccggcaat gccgatgatt ggtttcattt
    70441 ttattccccc taaagtagac taattgtaac aaggatttaa aaaaatttaa actatgtaag
    70501 acttgcccct atacttatct gttcttgctg tgttactatc tgtaaagaga attttcgtgt
    70561 gaaattcatg acgatagttg aaaatattgc ttaatttgtt ataataaaaa gtaagcagaa
    70621 ataaaaaggt tgggcgtaag cttcaaggat ctagttcttc aaggggtgag aaggacattt
    70681 tattgaaggg gcctgcctat actaagttat ctttagaggt acatactttc aatgaaaaaa
    70741 gaaagcatgt acctcttttt taaatcgaaa ggaagtgaag caagtgttag aagctttgga
    70801 acaaatccga aaagcagaag aagaaaatga gaagcgtgct cgaaccctac ggcagcagtt
    70861 gaagacctat gcagaagaaa aagagcaaga attggagata atccaaacga acagtcggca
    70921 acagctgaaa cagttggtag aagaaaaaga acaattcgaa aagcaagaat tagaaaaaga
    70981 aaaaatgaaa ttgaaagcag aagcagacaa ggtgcaagca actttgaaag accaatatca
    71041 aaagcatcag agtcaagcaa tcggtgcaat cattgaaagg gtgaaagaaa cttatggccg
    71101 tcactaagat ggaaaaagtg accctcattt cagataaaag aaatcaggag aggattttac
    71161 aagcagtaca aggaatccaa aatgttgaaa tccgggatct gtatcaaaat agaacaaaca
    71221 accagtgggt agaaaaatat tttccagata ccacgattat tgataaagaa gcaaaattaa
    71281 gcgctttagg caatcggctg aaagacatcc aagaagctat ccaattcata caacaccacg
    71341 gcgatagccg tcaaaagaaa ctccatttga aacgtcaaga actttcctta caagacttgg
    71401 aagcaaatta ttccgaagaa aatttcatga ataaattaga agaagtcctt gatttaaaag
    71461 aaaagtggga acagctaact gaaaaacgtg atcgtttaga agaagaggaa gaatggcttc
    71521 ttcattggca acagctggat attgctccaa gtatgtatgc aagtaagacg accatttttg
    71581 agatggggac tgtgagtgca gcaaattttg aaagtttcaa ggaagaacta agtaagcttg
    71641 atgacgttta tatggaagaa atagattata cgccaaaaca agtttatctt tcgtatgttg
    71701 ctttaaaaaa atcagttgct gctatagaag aaattgctgg gaaatacggg ttcaataaag
    71761 aaacgtatcc ttatgaacaa aatcctaaag aaaaattgca agaaaccaaa cagcaattat
    71821 ccattgttca cgagcagcaa aaaaagataa cgacagcgtt aggtcagtgc agcggctata
    71881 tccaagaatt tgaatgggta gaagaagtca cacttgccgt tgcagaacgg gaaaaagtaa
    71941 aagaacggtt tatccatgcc acttatctga tcgttttgca aggttgggta gatgcagaag
    72001 aaaaacagaa tttgatgcat gtgttaaacg aaactgtatc agaagaagat atatacgtta
    72061 gtttcgaaac accaaactca gaagagattc agcaggaagt ccctactaaa ttgaaaaacc
    72121 atccgcttat cgaacctttt gaattgctga ctgaaatgta cagtctgcca aaatatgaag
    72181 aagtagatcc aacgccttgg atgacaccat tttatcttgt cttcttcggg atgatggtcg
    72241 cagatgttgg ttacggattg ctgatgctca ttgggacaat actagctcaa aaactatttg
    72301 ttttaccccg cgggatgaaa cgtttcgtaa aattctttga gattctctcg gtaccatcga
    72361 tcatctgggg attgatctac agttcatttt tcggacaggc attaccaaaa gaactatttg
    72421 gtatccgctt gcctttccca atcctatcta cgacggatga cgtcaatacg attttgattt
    72481 tatccgtgat tttcggtctg atacagatct tggttggatt attcgtggca gcaaaggaaa
    72541 atatcaggag gaaagattac ttgagtgctg tcagtgatgg ttttgcctgg caagggatcc
    72601 tgattgggat cgttattgca ttgattggtg tcatgttgat aaaaaataca gcatttgttt
    72661 atcttggcgc ggggctatcg atcattgctg ctttatgtat cttaatcgtg ccgattatcc
    72721 aatcgccgtc taaagtcaag ggtgctgcca aaggagcgta taatctttac ggattgactg
    72781 gttatatcgg tgatttagtc agttatacac gtttgatggc actaggtatt tcaggcggaa
    72841 gtattggcgc ggcattcaat atgctagttg ctttcatgcc gccagccgct cgattcagtg
    72901 tagggatcct tttgatcatc gctttacacg cactgaatat gtttttgaca ctgttaagtg
    72961 cctatgtcca tggtgcacga ttgcagtatg tcgaattttt tggaaaattt tatacaggag
    73021 gcggacgtgc ctttgacccg ctgaaaacgg cggaaaaata cgtcaacatc aatcataaga
    73081 aaaaaaacaa taaataattg gaggattttt atacaatgat ggattactta attagtcaaa
    73141 atggtggaat ggtatttgca gtattagcga tggcaacagc gacgattttt tcagggattg
    73201 gttcagctaa aggtgttgga atgacaggtg aggcagcagc agcgttaaca acaagtcaac
    73261 ccgaaaaatt cggacaggct ttgattctgc aattgcttcc aggtacgcaa gggttgtatg
    73321 gattcgttat cgcctttttg atttttatca acttaggaaa tgatatgtca gttgtacaag
    73381 gattgaactt tctaggcgct tctctgccga ttgcttttac aggtttgttt tcaggtatcg
    73441 cccaagggaa agttgcagca gcaggtatcc aaattttagc gaaaaaacct gaacatgcta
    73501 caaaaggaat catttttgct gcgatggttg aaacatatgc gattttaggt ttcgttattt
    73561 ccttcctatt agtattgaat gcctaagcat aaaggaggaa aagcagatga acgccattga
    73621 aaaaatcatc tctcagatga atgaagcagc agagcaagaa agagcggcgt tagagcaaga
    73681 agagcgaatg aagatcgatc aaaactttga acaaaaacgt acgcaaatcg aaacagaaca
    73741 tcaaaaacaa aaagaaaaac aaatcgaatt acttgaaaaa aaataccgtc agcttaggaa
    73801 tcgccaacaa gtagaagtac gacaagaaaa tttgaatgcg aagcaggaat ttttacgacg
    73861 attgtttgca gatgcaatag ctgaaatgga gaattgggat gaatctgaac aaatccagtt
    73921 tatcaaaaat tcattgtatt cattaccttt gacaggcaaa gtaacattca ttgctggaga
    73981 aaaatcagca gcttatcttt ctcagacatt gctggatgaa tggaacaatg aacttccctt
    74041 tatgatggtt ttaagtgatg aaactgttgc tgatcaagcg ggttttctga taaacgatca
    74101 aggagttcag tataattttc ttttcagcag ccttgtacaa gatatccaag gaacgatgag
    74161 ttttgagatt gccaatcaat tattcgaata aggagggatc agtgtatgga ataccacgaa
    74221 ctaaatccct tgattcgcgg aagagagctt gaacttttgt ccaatgagac atttgaacgg
    74281 atgattcaaa caaattctgt cgaagcactt ggtgaaatat tgaaatccac gatttacagt
    74341 ccatatattt atgacggata tgaatatgac ttcgaacaga atcttttaaa ggagcagagc
    74401 cgtctatttc gctggttaaa ggaatcagca ccggagcctg aaatcgtttg gatctatacg
    74461 atgcgctata ctttccataa tctgaaggtt ttgacaaaag ccgagatgac aaatcaaaat
    74521 ctggaccatt tgtatatcga cgatggattt tattcattag aaaccttgaa ggatgccatt
    74581 catacgcaag tttcaaatga actccctgaa agcattatgg acagtatacg agaagtacgt
    74641 gattattttg aagagtctac cattcttcag ggaattgacg tgatttatga tcgacagttt
    74701 ttgacagagc agcgacggct tgccgataag ctaggatatc aagaattgct gacagaagtc
    74761 attgctttta ttgacttgac caatatcaca acgatggctc gaggaatatt gcagaaaaga
    74821 acatatgggt tcatgacgac ggtactttcc agctcgggaa gtatcgaaaa agaagtattt
    74881 ttgaactttg tagataaaga tatggaagat tacacgaatt ttctattaga gacagattat
    74941 gctgattttc tgcgccctgt catccgtgag ggaaatcttg atctcgtaaa gcttgaacag
    75001 ttgaaagatg attatctaag ttcattgtat caagaagcac aaacccaggc tttcggacca
    75061 ctgccactac tagccttttt gaacgcaaaa gaggtagaaa gcaaaaacct tcgtttactg
    75121 gttatcggca aaagaagcca tttcgaacca gaaacaatca gaaagcgggt gagacaagtc
    75181 tatgacgcat aagatcggtg tagttggcga caaagattct gttttgccat tcaaattgtt
    75241 tggctttgat gttcgttatg gtacaaccaa gcaaaccgtt cgtcaggcaa ttgaagaaat
    75301 ggcaaagaat gagtatggcg tgatttatgt cacagagcaa tgtgcagcat tgagcccaga
    75361 aacgatcgaa cgttacaagg aacaaatgat acctgctatc gtattgattc ccaatcatca
    75421 aggatcatta gggatagggc tggaagaaat tcaaaaaaac gtagaaaaag cagtaggtca
    75481 aaatatctta tagtatgaaa aggagataga aattttgcaa attggaaaaa tcataaaagt
    75541 ctccggacct cttgtaatgg ctgagaacat gtctgatgcc agtatccaag acatgtgtct
    75601 agtgggagat ctaggagtta tcggagaaat tatcgagatg cgtggcgatg tggcgtctat
    75661 tcaagtatat gaagagactt ctggaattgg tccaggcgaa ccggttcgct ctactggtga
    75721 agctttatct gttgaattag gaccagggat catttcacaa atgtttgacg gaatccaacg
    75781 gccattagat acctttatgg aaatcacaca aagtaatttc ttagggcgcg gagttcaatt
    75841 gcccgcattg gaccatgaga aaaaatggtg gttcgaacca acagtagaag ccggagaaac
    75901 ggtgagtgct ggagatgtta tcgggatcgt agaagaaaca aaagttataa aacataaaat
    75961 catggtgcca aatgggataa aaggaacgat caaaacaatc gaaaaaggtt cattcactat
    76021 tgatgaaaca gtatgtgtca ttgaaaccga aaatggtgac aaaaatttga cgatgcttca
    76081 aaaatggcca gtaagacgta gtcggccaat caaagaaaaa ttaaacccgg atgcacccat
    76141 gatcactgga cagcgagtga ttgatacatt cttcccagtg acaaaaggag gagcagcagc
    76201 tgtacctggt ccttttggtg cagggaaaac agtcgttcag catcaaattg caaaatgggc
    76261 cgatgtggac atggttgtct atgtgggatg tggcgagcgt ggaaatgaga tgacggatgt
    76321 tttaaatgaa ttccctgaat tgattgaccc taatacagga gaatcgttga tggaacgtac
    76381 ggtattgatt gctaatacat caaacatgcc agtagcagct cgagaagctt ctatctatac
    76441 gggaatcaca attgcggaat atttccgtga catgggttat gacgttgcta tcatggcaga
    76501 ctccacttca cgatgggcag aagcacttcg tgagatgagc ggacgtttgg aagaaatgcc
    76561 tggtgatgaa ggttatccag cttatcttgg ttcacggctt gcggaatact atgaacgatc
    76621 tggaagagtc attgcattag gatcagaagg tcgcgaagga agtataaccg caatcagtgc
    76681 tgtatccccg tctggagggg atatttcgga accagtcaca caaaatacat tgcgggtcgt
    76741 aaaagtattc tggggtcttg attccagttt agcccaaaaa agacattttc cttcgatcga
    76801 ttggatacaa agctattctc tttattcaac agaagtaggg aagtatatgg atcatatcct
    76861 tcaagctgac tgggcatcca tggtcaccga gggcatgcgt attttgcagg aagaacagca
    76921 gctgaatgaa atcgtgcgtt tggtagggat tgattccctt tcagataatg accgtctgac
    76981 attagaagtg gcaaaatcca ttcgtgaaga ttatttgcag caaaatgctt ttgatgatgt
    77041 cgatacattc acttcaagag agaaacaatt caaaatgttg aagatgatct tgacctttgg
    77101 tgaagaagga cgccgtgcgt tgtcgttagg tgcttattta agtgagatca tggacggtac
    77161 cgtatcgatt cgtgaaagga tcggacgaag caaatatatc ccggaaacag aattagaaaa
    77221 actagatcaa attattggtg aaatcaagca aacgatgcaa acgatcgttt cagaaggagg
    77281 catgagcaat gattaaagaa tatcgtacga tcggtgaagt cgtcggtcca ttgatggcgg
    77341 ttgaaaaagt tgctggtgtc aaatatgaag aattaatcga agtccgtatg caaaatggcg
    77401 agattcgtcg tggacaagta ttggaagtcc aagaagataa agcaatggtc caaatattcg
    77461 aaggaacaag tggtatcaat ttacgtgact cttctgtacg atttttaggc catcctttag
    77521 aactaggtgt atcggaagac atgatcggtc gagtattcga tggtttggga cgaccgaaag
    77581 ataacggacc agagatcctg cctgaaaagc atttggatat taacggagaa gtaatcaatc
    77641 cagtagctcg ggattatcca gatgagttta tccaaacagg tatctctgca atcgatcatt
    77701 tgaatacatt agttcgtggg caaaaattac cagtattttc tggctctggt cttcctcata
    77761 aagaattagc tgctcagatc gcgcgacaag caacggtact cgattcatca gatgatttcg
    77821 ccgtagtctt tgctgctatt ggaatcactt ttgaagaagc tgaatttttc atggaagatt
    77881 ttcgtcaaac gggcgcaatc gaccgttctg tcatgtttat gaatttagca aacgaccctg
    77941 caatcgaacg tatcgctact cctcgtatgg ctttgactgc tgcagaatat cttgcttatg
    78001 aaaagggaat gcatgttttg gttatcatga cagacatgac caactatgca gaggcattgc
    78061 gagagatttc tgcagcacgg cgagaagttc caggaagaag aggatatcct gggtatcttt
    78121 acacgaactt agctacttta ttcgaacgag caggaagaat ccgcggacta aaaggatcgg
    78181 ttactcaaat tccgattcta acaatgccag aagacgataa aactcatcca atccctgact
    78241 tgactggtta tattacagaa ggacagatca tcttgactag agagctttac aagagtggga
    78301 ttcagccacc aattgatgta ctcccgtctc tttcgcggtt aaaagataag ggaactggac
    78361 ccggaaagac aagagaagac catgcagcta cgatgaatca gctttttgca gcctatgcac
    78421 aaggaaaaca ggcaaaagaa ttagccgtag ttttaggtga atctgcttta tctgatgtag
    78481 acaagatcta tgcgaagttt gctgagcggt ttgaaaaaga atatgtcaat caaggattct
    78541 acacgaaccg ttcgattact gaaacacttg atctagggtg ggaactgcta tcgatgttgc
    78601 caagaaccga attaaaacga atcaaagacg acatgctgga caaatatctg ccagaaggga
    78661 agtgagttct gatggcacga ttgaatgtga atccgacccg catggaattg actcgattga
    78721 aaaaacagtt gacaactgcc acaagagggc ataaattatt aaaagataag caagatgaac
    78781 tgatgcgtca attcatcctg ttgattcgaa agaacaacca gctgcgtcaa gaaatggaag
    78841 agtctatgac agaagcaatg tctgattttg tattggcaaa tgcttcgatc acggaacctt
    78901 tcatcgaaga attatttgtt ttgccggcgg aaaacgttga tttgtctatc gtagacaaaa
    78961 atatcatgag tgtaaaagta cctgtgatga attttgaata tgatgaacag ctgacagaca
    79021 cacctttgga atacgggtat ttgaattcca gtgccgaact ggatcggtca atcgatcggt
    79081 ttactaccct gcttccaaaa cttttggaac tgacagaaat cgaaaaaact tgtcaactga
    79141 tggcgagtga aatcgaaaag acacgtcggc gagtcaatgc attagaatat atgacgatcc
    79201 ctcaattaga agaaacgatc tattatattc gtatgaagct ggaagaaaac gagcgcgcag
    79261 aagtaacaag gttgatcaaa gtaaaaaata tgggtaccac tgactaagtg gtatcttttt
    79321 ttaaaagagg aaaacgatga ttaaacaaag caaacaaata aagaaacgtt tgccggctgt
    79381 tcagcttctc gcattaggat tttttctcct gattttagtt ggcggcggcc tacttaccct
    79441 accatttttt agtaatagtg ggcatgggac aaattttatc gatgcgcttt tcactgcaac
    79501 ttctgctgtc tgtgtaacgg gattgacaac attgaataca gcagagcatt ggaacagtgc
    79561 tggtcaattt ttgatcatgc ttttgattga gatcggcggt cttggtttta tgatggttcc
    79621 gattctatgc tttgcattga taaagaagaa agtcagtctc agcacacgta ttatattaaa
    79681 agaagcgctg aatctagaag aagtatcagg tgtgatgaag ctcatgcttt acatcctaaa
    79741 attggccttt gttatccaat tgattggtac ggttgcttta tcttttgtat ttgtgcctga
    79801 atttggctgg ttgaaaggta tctggtacag tttgttccat gcaatctcta gtttttgtaa
    79861 tgcagggttt gatttgttag gagacagttt agccaatcac cagcaaaatg tttatttgat
    79921 catggtggta tctgctttga tcatttctgg tgggttaggc ttcatcgtat ggcgtgattt
    79981 actggaatat catcagaaaa agaagatgac gattcattca aaaatcgcat taaccgttac
    80041 aggtaccctt ttattaggcg gattcgttgt gttctttttc acagagcgca atggggcaac
    80101 gctagtggaa gggaatatgt ttcaacgatt ggcaaacacc ttttttatga gtgtgacacc
    80161 tagaacggct ggatattatt cagttgatta cctccatatg agccatgcag gtctgatttt
    80221 aacgatgttt ttgatgtaca tcggcggaac atctgggtcg acggccggcg gattgaaaac
    80281 aactacgtta ggtgtgttgc tgatccagat gcgttcgatg ttgaaaggac gtacgagagc
    80341 ggaagcattt ggccgtacca tccgacaagg cgcagtgttg cgggcactta ctttattttt
    80401 tgttacgttg acgttgtgtg tagtcgccat catgatttta tcggtgactg aaacgatacc
    80461 taaaacttct ggcatcgaat atattgcttt tgaggtcttc tctgcgtttg gtacagtagg
    80521 tcttacgatg ggattgacgc cagacttgac tgcaataggg aaattgatca ttatagcgct
    80581 tatgtacatt ggacgagttg gtgtattgac tgtggtcttc tctctgatga caaaagcaca
    80641 aagaacacaa tcaaaataca aatatccaga agaaagtgtc atgattggat aatttcatct
    80701 tctgacagaa acctagtgat ttagtaaaat aagaaaataa aataattttt cttgcttctt
    80761 ggatacttta agtgcaaaat gttcgtttat tttttgaggc actacccgtt gttgaacctc
    80821 gggtagtgcc tcttttttat tgaaaggaat caataagatt tgattccttt atttatcaat
    80881 aggttataat tcatattttt ttattttttc ttttacgata cttagtccag aagctaaaat
    80941 ttcaaggtta ttagaggata gggaaattcg aacgaatgca tctggttgcg cacgtgtagt
    81001 aaagcgtctg gaatgataaa gtcggatacc gttagcaaga aagtcattct caattatctt
    81061 agggtcaatc tcaggatcaa ctggaagaat tcgatagtaa gggacacgat tcgttggttt
    81121 ttctactttg aaaagagaat caaaaagctg attggctctt tttgtaagac ttaatttagt
    81181 ttctaagatc ctatttgctt catttgaatg gaggatttga gtgatgactt ccacatctaa
    81241 tccagaagtt ttcacattta tattaaaaat tgcttgttct atcttgcttt ggacatgtac
    81301 tggaactaca agataagcaa ctcgcaatcc agaacaaata aattttgtca ttccagctaa
    81361 gtagattgtt tggttaggta ataaattttg gaaaggagga atgatcgtat gttgatagta
    81421 agttgttaaa aatgaatgga tatcatcttc aataacccat agaccttctt tttcaatcac
    81481 ttcggctaat tgctctcgtc ttttgtgaga gatttgaaac cctataggat tattacaaga
    81541 aggcataaga aagacaccat gaatcttctt ttttctacat tcctgaatca atatatcagg
    81601 tcgcattcct tgctcgtcaa aatcaattgg aacgatctct aaatgatata actgagccaa
    81661 ttcaatgaaa ttagcgtatg tatatcggtc tgttgctatt ctatcacctg gagagaaaag
    81721 tgcagaaagt gtgatagcca atccattttg aacaccggaa acaatagata gagattctaa
    81781 ggacggaatg actccttgag tttttaacca atttgctgca gtagtcaatt gaaaatttgt
    81841 gcctttggga tcttggtaat taagcaaacc aacaagattt gggttattac tagttgattg
    81901 aataaacggg acaacgaatt tattacattc ttcaaaagaa ctaaccagac caaaatcaat
    81961 gatatgttcg gttacttcat tggtagagat ggtgatggac tcaaagacat tgggagaaat
    82021 aaatgtgcca cttccaatat tcgtatacag caaacctttc tcaattccat atttataagc
    82081 cttgccaacg gtagtaaaat ttaaatctaa ataatcagct agctctcttt gggagggtaa
    82141 ctgagtgttt ttctgtaatt tattctttaa aatatcttgt tctaattgtt gaatcaacga
    82201 tcgataataa ggacgcttca atttttgttt gtctggtcgc caagttaacg ggtaattgtc
    82261 ataagaatta ataggcattt tctccactcc ttaaaattgt ataccataca attttaccat
    82321 tgattgtatt gattttagaa gttaatcttt tgtgaggagg gaattgtcta tgaaaattat
    82381 taattttgag gaagtagtag cttcttttag ttttgaagaa gcgattcaag taatgaaaaa
    82441 atgttttttt gattatgaaa gtggtaaaat cagccaaaat cctcgattgg tggaaatttt
    82501 acctgacgga aaaggcaaaa atatgtttgc gatgatgcct gcttatttgg gggagaacag
    82561 atattttggc acaaaagtca ttaccgcttt tccggataat catcagtatc atttaccttc
    82621 tcatttagga gagattttct tgtttgatgc tcaaaacgga ctacccaaag cattgataaa
    82681 cgcaaatgca gtcacctgga ttcgaacagc tgctgtatct gcgctggcaa cagattactt
    82741 agcaaatccg catgcagaaa cacttgcttt aattggagct ggtcaacaag cattgtcgca
    82801 tttgactgcg atttctacca ttcgtcccat cactacagtg tttgtttacg atattttaga
    82861 agaaagacga aatactttta tcgctaaagc taaaaaacag tatcctaaaa taacgtttat
    82921 aaatgcaata tcagtagaag atgcagtacg tccagcggaa atcatttgta cgttgacctc
    82981 tagtaaagag gcatttcttc aaaaagagtg ggtaagggaa gacgcacata tcaatgctgt
    83041 gggcacattt actcctgata caagagagat aagtacatcg ttgatggaaa cttctcaaat
    83101 ctatgtagat gattactcag ctgccttaac agaaagcggg gacctgctta tagccatggc
    83161 agaaggagat ttagaggaat cttgtattgt tggtacatta ggcgaattag tttctgagaa
    83221 aaaaacattt tataaaggag aatcgaggcc gacgattttc gacgcagttg gtctagctat
    83281 tgaagatctt tgttgtgcag aatatattta taacaaatta gagaaagggg ctttaaaatg
    83341 aacttagatt attacgtagt tgatgcattt gcagatgagg tgttcaaagg aaatccagca
    83401 gcggtttatg tgctagaaga atggctgcca gaaggtacga tgcaaaaaat tgccattgaa
    83461 aacaatttat ctgaaacagc atttacagtg aagaaaaacc aagaatttga gcttagatgg
    83521 tttacgccag atcgagaaat cgatctttgt ggtcacgcga cattagcaac agcatttgtt
    83581 ttatttaact attacaaaat acctgatgaa acgattaaat tttctactca aagcggtaat
    83641 ctatttgtga caagaattaa agattattat tacatggatt ttcctagtat tatgccgaaa
    83701 aaagtgccga tattgtctga atatgaagaa gcgattgggg ctaagattaa agaagcctac
    83761 ttagcacgtg atttattttt cgtattagaa gatgaaaaaa cggtggctaa attgcagcca
    83821 gattttacag ctatcaaaaa ttttgaatta ggaataggag tcatcgtgac agcagaaagt
    83881 gtgcaaaaag actttgtttc tagaaccttc tttccaaaat taacaatcaa tgaagatcca
    83941 gtatgtggat cagcacattc taatcttatt ccttattggg cagaaagaat gaataaaaca
    84001 aaattgtcag cttatcaaat gtcatcaagg ggcggggatc tagaatgtga attaaaagaa
    84061 gatcgtgtga taataggagg aaaagcagtt ttgttttcta aaggtacctc gtttatttga
    84121 tttctttcaa atgaattaaa aataaattgg tggagagtgg caaccttata gctacaatgg
    84181 ttgttaaaaa aagacgcaaa ctttttcaat ttaatgaaaa gtttgcgtct cattttaatt
    84241 attggtgttt gttttttatt caatcaagag taaaatgccc catactaact atcctcggat
    84301 gacttcgatt ttataaccat cagggtcaat gacaaagtag taagaaggcg cagtgccagg
    84361 taatcctttt aagtcagtaa tctcaagacc ggcagcttta tgttgctcgt gtaatttttc
    84421 tagatcatca gctgctatag cgatatgacc atagccgtct cctagatcat acgcaccatg
    84481 atcatagttg taagtcaact ctaattcata gtcatcccca ggaagtgtca aataaacaag
    84541 tgtaaatttg ttttctggaa aatcacggcg gcggctttct tcaaaaccaa atgccttttg
    84601 ataaaattcg attgatgctt caagatcttt cactcgaaca catgtgtgag ccattttcat
    84661 agattcatct ctccttttcc ttttactcaa tcataacaaa aagaagtaag gaaatagttg
    84721 atttgtgctt gttcatgaga aatttctcat gtaaaatgga tagaaaataa aactgttaag
    84781 gtggaaacta ttcatggata aaccaatttt tggtgaaaaa aagcaatttg ctgattatca
    84841 aacccgttat gcagcatata ttgtcataga aagagatcag cagattgcag taatcgaggc
    84901 tccaaatggt gctttttttc ttcctggcgg ggaaattgaa ggagaagaga cgaaagaaga
    84961 agcgattgtc cgtgagctga tagaagaaat gggaattgtg gcagaaattt cttcttatct
    85021 gggagaagct gatgagtatt tttactcgaa ttatcgtgca acttattatt ataatccagg
    85081 atatttcttt gttgctgatt cttggaagcg tataggagaa ccaactgaaa agaccaataa
    85141 aatttggtgg gtgagccctc aagaagcgct tgtgaaattg aaacgtggta gccaccgatg
    85201 ggcagtcgag agatggatcg aaaaaaataa aaactgaaag aaagcgagta gatcagcttt
    85261 ctctcagttt ttttatttat tacagtacat attttttgat tgcttcagct acaccgtgct
    85321 cgtcgtttga tgaagtgatg acatttgctg cttctttgac aagagaaacg gcatttccca
    85381 ttgctactcc tagaccagca tattcaatca tggataagtc gttctcgtta tctccgatag
    85441 ccatgatttc atcttgatca ataccaaggt gctgtgcgag attcgcaact gccgcacctt
    85501 tacttgcttc tttatttagt acttcgaagt aatagggcgc acttttgacc gttgtgtatt
    85561 tatcacggaa agtttgcggt aaacgcgcaa ttgctgcatc taaaatttct ggttcgtcga
    85621 tcatcatcat tttgacgatg ttcatgtctg gtgtcatttc ttcaggtgtt cgatatttta
    85681 gcggcatctt gactaaactt gcttcgtgga tcgtataagc gctgatatct cgattaggcg
    85741 tgtagatcgc tttgtcatcg atcgaatgga gatgactgcc gacacggcgt gccatcactt
    85801 cgatctctag gaaatcttca tgtgtcagtc cgtgacgggc gatgatttta cctgatttcg
    85861 tttgttgtac gagtgccccg ttataagtaa taacgtaatc attatcttga aaaaggttca
    85921 actcgtttaa ctgttcctgg acacctggta agggacggcc agtgcataaa acgatcgaaa
    85981 caccttttgc aacagcagcg gcaatcgctt cttttacttc ttttgttact tctcttttct
    86041 catttaataa agtaccatcg atgtcgatag cgactagttt gatagacata atttcctcca
    86101 atatatttta atttatttca atcaatgcgc cattacggat atggctggca aattcttgat
    86161 aagtttcgtc aaataaatca taatgatcac gcaaggacgg atcaaccatt tcttttggaa
    86221 agaaaaatcg ctcatctcct tgttcttgtc ccgctaatgc agcaacaagc tgactaacct
    86281 gtgataattc tatcaaagtt ccgtcatttc gcaaaatctc gatttgtgtg cgatgtctgc
    86341 cttgttctgg gcgataaaaa tcataaggta gatcgtagct agaattcaca gcagtgtaat
    86401 aaacaggatt atatccgacc ttttctacta acaaagtgag ttcttggact aattttgaat
    86461 cccggttgcc agaaaaagta gcagatttca gtggtttacg attcaaaaaa cgtttagcta
    86521 aatcatttaa aataggatca gcagattcag accattgagt aaagtaagtg cccaagaccc
    86581 catcatcaag tttgaggtat tcttctaaag taaagcttcc gtcaagaaat ggcagaagta
    86641 attgagaatg gagttcaaaa ctgctggcat cttcttgata caactcgtgt gcgcgatgaa
    86701 gcagatgctc caaaattact tccataccac gggaaacagg atggaagtac acttgcacat
    86761 acatttgata tcggctaacg atataatctt ccactgcatg catgccactc atggaaaaag
    86821 cgatcccgcc ttcataagga cgaatcacac gtaatattcg ggtaagatca aatgttccat
    86881 attctgttcc agtgaaataa gcatcccgaa gcaaatagtc cattcgatct gcatcgatct
    86941 gactagaaat catttggaca acttgtgggt tggggtaagt tttttggatc acactagcta
    87001 ccttttcagg aaaaccttct tctactcggt taagaatctg atacacttct gtttctggag
    87061 aagtgatgat ctctacagtg atggcttcat gattcgtatg gaagatatgt tcaaatgtat
    87121 gagagtaagg accatgaccg acatcatgca acagtgccgc acataaagtc accagccgtt
    87181 cctgatcatc ccagcctccg ttgcctattt tttcttttga aaaatttcga gaaaagatat
    87241 cgcagattcg tcttgaaatc tcataaacgc ctaatgaatg agtaaaacgg ctatgttctg
    87301 ctccgtgaaa agtgaaagaa gaagtgccta attgtttaat gcgtcgtaga cgttggactt
    87361 ctttcgaatt gatcaaatct aagattactt gatgctggac atggatatag ttatggactg
    87421 gatcacgaaa aactttttcc ataggcagca tttgatattt ataaggggtt gtcataatgt
    87481 agctccttta tgttattacg ttgttccatt ctctggattt ggttgttcca ttgctccagc
    87541 tggatttctt tttttagcca gtcggtagaa tcctgtaaat cacttgcaga atacagcttt
    87601 tcaaaagctt ttgcaaatgc ttctttgact tcttttacaa aaaggggttg gtcaagcagt
    87661 tcttcaagtg ttgccattgt agcaggatcc actgcaggat agccatttgt accaaagtct
    87721 tcttttaaag cagattgata aaatcgcttc atgacttgcc cgcgtttttc ctgattaccg
    87781 ttgacactta agtacatcat aacagaaatc ccatttttga tccgacgctg agcgataccg
    87841 gcaaattttt tgccatggat gcttaaatca taagttccag gacaataaga atgggtgatt
    87901 tcgaatgctt gtatcggtgc ctttggaaaa gcgtatttag tcaataggag catttgctcg
    87961 taagcgtcat ctatggttat tttttttgtg tcactttggg ggaaaatcaa agaaatattc
    88021 aaaattcctt catctgatac gatccctaat ccacctgaat ttctgatcac tggagcgtaa
    88081 cctgcatctt tcagtgtttg gatcccgtca cttagaaaag gagttcgaac atccttcata
    88141 cctaagatga ctgtgcagtc atattgccag aaatgtaaaa agatttgctg gtgttcttta
    88201 gaatattccg ttaatatatc tgttaatgca aaaggggaaa agtcgttttt ttgataaatg
    88261 tgctgatcta gtcgaatcgc agggttcatt caaccggctc ctttgtataa attcatattt
    88321 tctacaatat tatactctaa aatcaaagag ttgttttgga aaaaaggttt tctgtaaaaa
    88381 tggacaagtt ggttgacaaa acaaaagaaa ctaggaacaa tgtagctatg agaagtaaag
    88441 gagaatgaat aatggaatta tcacaaggaa caccgatttc aatcaaattg cgtacaaaag
    88501 taaaacagca aggggaagtg caagattttt attttgattt gaaagggcaa atggtgacaa
    88561 ttggcgatac gctgtacata cgttataaag agattcagga ggagacgggc gatgagattc
    88621 ctgtaacgat caaattagtt ccagatggtc atgtacagct gatccgtgca ggagaaatgc
    88681 ggatgcgttt gaaatttggc tataaagaac ggttagaaac atcctatcga acaccatacg
    88741 gtatgatcca gattgctact tttacgaaag aactgcatgt tagtttgaaa gaccgtccca
    88801 caggagggaa ggttcgaatc gattatgatc tgttcatggg accggaaaaa atcggcgaat
    88861 attatttgac cttggatttt acagcgtaaa tataaaagaa aagtttgatt ttttgtgtct
    88921 gattttgtat gataaggagt agactgtgaa aggacgtgtc ctgattggaa attagtgtat
    88981 ttgaaggtgc aaacaaaagc gaattatcaa tgatcgaagt agcacacgcg atcttagaac
    89041 aacgtggcga tgtcatggat ttttctgatt tagctaacca gatccaaaac tatcttggga
    89101 aatcagacag tgaaattcgt gattcactcg cacaatttta tactgacttg aatattgacg
    89161 gcagctttat ctcattaggg gataatcgtt ggggcttgcg ttcatggtat gctatcgatt
    89221 cgatcgatga agaagtaaac catggaatgg acgaagagga tgaagatact ccacgtcgcc
    89281 gtaaacgcaa gaaagtcaac gcctttatca atgatgatga agacgcgatc gattacaatg
    89341 acgatgaccc agaagataca gatctgacag aagaagatga cgatgatctg tttgatgacg
    89401 atgatgatga agatgaagaa atcgctgctt ataattctga cttgcaagaa atcggagcag
    89461 atgacccgac agatgatgaa gaagacttgc caggaaatat cgaagaagat ttaagtatca
    89521 tagacgaaga cgatgatgat gaagattatg aagaggaaga attttctgac tctgaaaaag
    89581 aataaacatt attttcttac gagaggttgt gacagagacg atcagcctat cagaataagg
    89641 aggtgtgaca cgat
//
LOCUS       DAZULO010000002        81815 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000002 DAZULO010000000
VERSION     DAZULO010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 81815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.002"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..570
                     /locus_tag="ACG8OA_000091"
     CDS             52..570
                     /locus_tag="ACG8OA_000091"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS4974943.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            698..1381
                     /locus_tag="ACG8OA_000092"
     CDS             698..1381
                     /locus_tag="ACG8OA_000092"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFS4974944.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1690..1763
                     /locus_tag="ACG8OA_000093"
     tRNA            1690..1763
                     /locus_tag="ACG8OA_000093"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1724..1726,aa:Arg,seq:tct)
     gene            1922..2281
                     /locus_tag="ACG8OA_000094"
     CDS             1922..2281
                     /locus_tag="ACG8OA_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFS4974945.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2404..4035
                     /locus_tag="ACG8OA_000095"
     CDS             2404..4035
                     /locus_tag="ACG8OA_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFS4974946.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4097..4738)
                     /locus_tag="ACG8OA_000096"
     CDS             complement(4097..4738)
                     /locus_tag="ACG8OA_000096"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS4974947.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4924..5000
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5020..5328
                     /gene="rplU"
                     /locus_tag="ACG8OA_000097"
     CDS             5020..5328
                     /gene="rplU"
                     /locus_tag="ACG8OA_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFS4974948.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5355..5699
                     /locus_tag="ACG8OA_000098"
     CDS             5355..5699
                     /locus_tag="ACG8OA_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFS4974949.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5712..6005
                     /gene="rpmA"
                     /locus_tag="ACG8OA_000099"
     CDS             5712..6005
                     /gene="rpmA"
                     /locus_tag="ACG8OA_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFS4974950.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6439..9246
                     /locus_tag="ACG8OA_000100"
     CDS             6439..9246
                     /locus_tag="ACG8OA_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4974951.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9437..9928
                     /locus_tag="ACG8OA_000101"
     CDS             9437..9928
                     /locus_tag="ACG8OA_000101"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS4974952.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10031..11020
                     /locus_tag="ACG8OA_000102"
     CDS             10031..11020
                     /locus_tag="ACG8OA_000102"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS4974953.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11053..11856
                     /locus_tag="ACG8OA_000103"
     CDS             11053..11856
                     /locus_tag="ACG8OA_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFS4974954.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11877..12791
                     /locus_tag="ACG8OA_000104"
     CDS             11877..12791
                     /locus_tag="ACG8OA_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4974955.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12874..13248
                     /locus_tag="ACG8OA_000105"
     CDS             12874..13248
                     /locus_tag="ACG8OA_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFS4974956.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13387..13632
                     /locus_tag="ACG8OA_000106"
     CDS             13387..13632
                     /locus_tag="ACG8OA_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4974957.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13804..14868
                     /locus_tag="ACG8OA_000107"
     CDS             13804..14868
                     /locus_tag="ACG8OA_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFS4974958.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15114..15539
                     /locus_tag="ACG8OA_000108"
     CDS             15114..15539
                     /locus_tag="ACG8OA_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4974959.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15532..15981
                     /gene="nusB"
                     /locus_tag="ACG8OA_000109"
     CDS             15532..15981
                     /gene="nusB"
                     /locus_tag="ACG8OA_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFS4974960.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16078..16932
                     /locus_tag="ACG8OA_000110"
     CDS             16078..16932
                     /locus_tag="ACG8OA_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFS4974961.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16933..18270
                     /gene="xseA"
                     /locus_tag="ACG8OA_000111"
     CDS             16933..18270
                     /gene="xseA"
                     /locus_tag="ACG8OA_000111"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFS4974962.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18275..18499
                     /locus_tag="ACG8OA_000112"
     CDS             18275..18499
                     /locus_tag="ACG8OA_000112"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFS4974963.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18501..19391
                     /locus_tag="ACG8OA_000113"
     CDS             18501..19391
                     /locus_tag="ACG8OA_000113"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS4974964.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19400..20218
                     /locus_tag="ACG8OA_000114"
     CDS             19400..20218
                     /locus_tag="ACG8OA_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFS4974965.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20325..20774
                     /locus_tag="ACG8OA_000115"
     CDS             20325..20774
                     /locus_tag="ACG8OA_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS4974966.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20792..22474
                     /gene="recN"
                     /locus_tag="ACG8OA_000116"
     CDS             20792..22474
                     /gene="recN"
                     /locus_tag="ACG8OA_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFS4974967.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22529..22645)
                     /locus_tag="ACG8OA_000117"
     CDS             complement(22529..22645)
                     /locus_tag="ACG8OA_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFS4974968.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22718..23674)
                     /locus_tag="ACG8OA_000118"
     CDS             complement(22718..23674)
                     /locus_tag="ACG8OA_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS4974969.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23845..24213
                     /locus_tag="ACG8OA_000119"
     CDS             23845..24213
                     /locus_tag="ACG8OA_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFS4974970.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24383..24814
                     /gene="mraZ"
                     /locus_tag="ACG8OA_000120"
     CDS             24383..24814
                     /gene="mraZ"
                     /locus_tag="ACG8OA_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFS4974971.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24835..25794
                     /gene="rsmH"
                     /locus_tag="ACG8OA_000121"
     CDS             24835..25794
                     /gene="rsmH"
                     /locus_tag="ACG8OA_000121"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFS4974972.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25799..26200
                     /gene="ftsL"
                     /locus_tag="ACG8OA_000122"
     CDS             25799..26200
                     /gene="ftsL"
                     /locus_tag="ACG8OA_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFS4974973.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26201..28393
                     /locus_tag="ACG8OA_000123"
     CDS             26201..28393
                     /locus_tag="ACG8OA_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS4974974.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28419..29381
                     /gene="mraY"
                     /locus_tag="ACG8OA_000124"
     CDS             28419..29381
                     /gene="mraY"
                     /locus_tag="ACG8OA_000124"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFS4974975.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29395..30756
                     /gene="murD"
                     /locus_tag="ACG8OA_000125"
     CDS             29395..30756
                     /gene="murD"
                     /locus_tag="ACG8OA_000125"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFS4974976.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30782..31870
                     /gene="murG"
                     /locus_tag="ACG8OA_000126"
     CDS             30782..31870
                     /gene="murG"
                     /locus_tag="ACG8OA_000126"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFS4974977.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31885..33105
                     /locus_tag="ACG8OA_000127"
     CDS             31885..33105
                     /locus_tag="ACG8OA_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFS4974978.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33261..34586
                     /gene="ftsA"
                     /locus_tag="ACG8OA_000128"
     CDS             33261..34586
                     /gene="ftsA"
                     /locus_tag="ACG8OA_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFS4974979.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34609..35850
                     /gene="ftsZ"
                     /locus_tag="ACG8OA_000129"
     CDS             34609..35850
                     /gene="ftsZ"
                     /locus_tag="ACG8OA_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFS4974980.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35867..36544
                     /locus_tag="ACG8OA_000130"
     CDS             35867..36544
                     /locus_tag="ACG8OA_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFS4974981.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36558..37157
                     /locus_tag="ACG8OA_000131"
     CDS             36558..37157
                     /locus_tag="ACG8OA_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFS4974982.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37238..37459
                     /locus_tag="ACG8OA_000132"
     CDS             37238..37459
                     /locus_tag="ACG8OA_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFS4974983.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37508..38290
                     /locus_tag="ACG8OA_000133"
     CDS             37508..38290
                     /locus_tag="ACG8OA_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFS4974984.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38311..39018
                     /locus_tag="ACG8OA_000134"
     CDS             38311..39018
                     /locus_tag="ACG8OA_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFS4974985.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39280..42078
                     /gene="ileS"
                     /locus_tag="ACG8OA_000135"
     CDS             39280..42078
                     /gene="ileS"
                     /locus_tag="ACG8OA_000135"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFS4974986.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42127..43653)
                     /gene="zwf"
                     /locus_tag="ACG8OA_000136"
     CDS             complement(42127..43653)
                     /gene="zwf"
                     /locus_tag="ACG8OA_000136"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFS4974987.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43668..44315)
                     /locus_tag="ACG8OA_000137"
     CDS             complement(43668..44315)
                     /locus_tag="ACG8OA_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS4974988.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44499..44828)
                     /locus_tag="ACG8OA_000138"
     CDS             complement(44499..44828)
                     /locus_tag="ACG8OA_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFS4974989.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            45005..45733
                     /gene="sfsA"
                     /locus_tag="ACG8OA_000139"
     CDS             45005..45733
                     /gene="sfsA"
                     /locus_tag="ACG8OA_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFS4974990.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45749..46762
                     /locus_tag="ACG8OA_000140"
     CDS             45749..46762
                     /locus_tag="ACG8OA_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4974991.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46762..48039
                     /locus_tag="ACG8OA_000141"
     CDS             46762..48039
                     /locus_tag="ACG8OA_000141"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4974992.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48102..50804
                     /locus_tag="ACG8OA_000142"
     CDS             48102..50804
                     /locus_tag="ACG8OA_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4974993.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50956..51273
                     /locus_tag="ACG8OA_000143"
     CDS             50956..51273
                     /locus_tag="ACG8OA_000143"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4974994.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51303..51623
                     /locus_tag="ACG8OA_000144"
     CDS             51303..51623
                     /locus_tag="ACG8OA_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4974995.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51731..53191
                     /locus_tag="ACG8OA_000145"
     CDS             51731..53191
                     /locus_tag="ACG8OA_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4974996.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53259..53480
                     /locus_tag="ACG8OA_000146"
     CDS             53259..53480
                     /locus_tag="ACG8OA_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4974997.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53511..53693
                     /locus_tag="ACG8OA_000147"
     CDS             53511..53693
                     /locus_tag="ACG8OA_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFS4974998.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53693..54106
                     /locus_tag="ACG8OA_000148"
     CDS             53693..54106
                     /locus_tag="ACG8OA_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFS4974999.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54229..55410
                     /locus_tag="ACG8OA_000149"
     CDS             54229..55410
                     /locus_tag="ACG8OA_000149"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4975000.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55481..55645)
                     /locus_tag="ACG8OA_000150"
     CDS             complement(55481..55645)
                     /locus_tag="ACG8OA_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975001.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55799..55872
                     /locus_tag="ACG8OA_000151"
     tRNA            55799..55872
                     /locus_tag="ACG8OA_000151"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55833..55835,aa:Arg,seq:cct)
     gene            complement(55918..56031)
                     /locus_tag="ACG8OA_000152"
                     /pseudo
     CDS             complement(55918..56031)
                     /locus_tag="ACG8OA_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(56414..57646)
                     /locus_tag="ACG8OA_000153"
     CDS             complement(56414..57646)
                     /locus_tag="ACG8OA_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFS4975002.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            57900..58469
                     /locus_tag="ACG8OA_000154"
     CDS             57900..58469
                     /locus_tag="ACG8OA_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4975003.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(58647..59087)
                     /locus_tag="ACG8OA_000155"
     CDS             complement(58647..59087)
                     /locus_tag="ACG8OA_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFS4975004.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            59245..60009
                     /gene="map"
                     /locus_tag="ACG8OA_000156"
     CDS             59245..60009
                     /gene="map"
                     /locus_tag="ACG8OA_000156"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFS4975005.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            60041..60964
                     /locus_tag="ACG8OA_000157"
     CDS             60041..60964
                     /locus_tag="ACG8OA_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFS4975006.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            61040..62179
                     /locus_tag="ACG8OA_000158"
     CDS             61040..62179
                     /locus_tag="ACG8OA_000158"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFS4975007.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            62172..62972
                     /locus_tag="ACG8OA_000159"
     CDS             62172..62972
                     /locus_tag="ACG8OA_000159"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4975008.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            62972..63799
                     /locus_tag="ACG8OA_000160"
     CDS             62972..63799
                     /locus_tag="ACG8OA_000160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4975009.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            63804..64511
                     /locus_tag="ACG8OA_000161"
     CDS             63804..64511
                     /locus_tag="ACG8OA_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4975010.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            64611..65477
                     /gene="rfbA"
                     /locus_tag="ACG8OA_000162"
     CDS             64611..65477
                     /gene="rfbA"
                     /locus_tag="ACG8OA_000162"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFS4975011.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            65491..66063
                     /gene="rfbC"
                     /locus_tag="ACG8OA_000163"
     CDS             65491..66063
                     /gene="rfbC"
                     /locus_tag="ACG8OA_000163"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFS4975012.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            66085..67113
                     /gene="rfbB"
                     /locus_tag="ACG8OA_000164"
     CDS             66085..67113
                     /gene="rfbB"
                     /locus_tag="ACG8OA_000164"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFS4975013.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            67211..68062
                     /gene="rfbD"
                     /locus_tag="ACG8OA_000165"
     CDS             67211..68062
                     /gene="rfbD"
                     /locus_tag="ACG8OA_000165"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFS4975014.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            68097..70130
                     /locus_tag="ACG8OA_000166"
     CDS             68097..70130
                     /locus_tag="ACG8OA_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975015.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            70174..71454
                     /locus_tag="ACG8OA_000167"
     CDS             70174..71454
                     /locus_tag="ACG8OA_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFS4975016.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            71664..72470
                     /locus_tag="ACG8OA_000168"
     CDS             71664..72470
                     /locus_tag="ACG8OA_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4975017.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            72482..73702
                     /locus_tag="ACG8OA_000169"
     CDS             72482..73702
                     /locus_tag="ACG8OA_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4975018.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            73692..75278
                     /locus_tag="ACG8OA_000170"
     CDS             73692..75278
                     /locus_tag="ACG8OA_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4975019.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            75317..77455
                     /locus_tag="ACG8OA_000171"
     CDS             75317..77455
                     /locus_tag="ACG8OA_000171"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4975020.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(77823..78806)
                     /locus_tag="ACG8OA_000172"
     CDS             complement(77823..78806)
                     /locus_tag="ACG8OA_000172"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS4975021.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            79184..80575
                     /locus_tag="ACG8OA_000173"
     CDS             79184..80575
                     /locus_tag="ACG8OA_000173"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS4975022.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            80591..81631
                     /locus_tag="ACG8OA_000174"
     CDS             80591..81631
                     /locus_tag="ACG8OA_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4975023.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            81650..>81815
                     /locus_tag="ACG8OA_000175"
     CDS             81650..>81815
                     /locus_tag="ACG8OA_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFS4975024.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERL"
BASE COUNT        26697 a        13826 c        17345 g        23947 t
ORIGIN      
        1 atcgttatta tatgattaag aaggattgaa taaagaaaga agttgaaaac gatacctgat
       61 tacaaaaaaa taacttttat aagttctata acgttgatta tatttttaat tcttattctt
      121 ctcgtcgtct tggttccccg aatttttggt ttgactcctt atattattga ggacaaagaa
      181 atgagtccat attatgcaca aggaaatctg atatatgtac gaccgaaaaa tacaaatgag
      241 attttagtcg gagatattat tacatattat gaaaattcag gaaaaaaaat ctcgacgcgt
      301 cgcgtaatag ccgttgaaaa caaccacgaa gatttttata caaaagctga tacaaagact
      361 tatattgagc ctggagtagt aaaaaagaga aatattatcg gtagtcccat ctttcagatt
      421 ccatatattg gactagtcct ttcaaaaacg gcatttgcat ggattcaggg gttattcttt
      481 acgattgctt tttgcctaac aggaatcaca gcatggaata cctttgtaga gttgaaaaag
      541 aagcggagac gaacacagaa atttttgtaa gaatgttaaa aacataaatg tattccagag
      601 atccaacgac tcttctgaac atgaaacacg agaaaacact catgcagctt gtagatttag
      661 tcagttgttc gtataatgtg tgaagaggag gagtagtatg cgcaatgaaa tgatgcatcg
      721 tcctgtagtc aaagaagaac tattggattt tatgcgtacc cagcaaaaaa aattaccggg
      781 tgagctaggc aaactggaag aagaagctca tgtagcagaa gtaccagtta ttccccatga
      841 aacagttgta tttttgaaat ttttacttgg acagcttcag cctgaacgaa tcttggagat
      901 cggagcagct atcggttttt catcaagtgt catggccacg acaatcggag aaaacgctca
      961 tgttacgaca attgatcgtt tcgatgtcat gatcgagaaa gctaaaaaaa actatgaacg
     1021 tctaggactg acagataaag tgactttgct tgaaggtcaa gcagcagata tcttgccgga
     1081 gttgtctggt ccgtatgact ttatttttat ggatagtgca aaatcaaaat atattgaatt
     1141 tttacctgaa tgcttacgtt tgttaagaaa aggtggcgtt ttgatggtag atgacatctt
     1201 ccaaggagga acgatcttgc tcccagatga agagattccc cgtggcaaac gagctatcca
     1261 tcgtaaatta aatgaatttc ttcgtgttgt gatggatcat cccgatttga catcgacaat
     1321 cttgccatta ggtgatggtg tgatcttaat gacaaaggaa tcagaaacaa tcgagttgtg
     1381 atacatgatt atggcagcct gtagataatc gattgaatcg agggctcaat tcgaacttga
     1441 tgtaaaagtt tctatatttg taagtcaatt aaaggataca ggaaatctaa ataaacagga
     1501 gtagcatgaa gtcaatgtag ctcctgtttt caattgataa ccttagaaca gctgaaggag
     1561 aatgtctcgt tttaaaagat tgatttagta gttgggccaa aataattcat tatttgctaa
     1621 ttaaataact atttcgtatt gacagaagca atttaattat ctataattat tgatgttccg
     1681 ctgattcttg tcatagtagc tcagctggat agagcatccg ccttctaagc ggacggtcgg
     1741 gggttcgaat ccctcctgtg acgctaaaaa accgttagaa actgaggttt ctaacggttt
     1801 ttctttgttt tttgtgccaa tattgtttgt tttcaaaggc aaataaaata aaaaaacatc
     1861 cttatcgagt acaattataa ggagcaaaaa aataaaactc atcagaaaag aggaagaaga
     1921 tatgacgata ctatctagaa ttttagtgac tctagttgca attgaatttt tttatatcat
     1981 gtatatcgaa acggtgagaa ctgattctga cacgacaagt agggttttta aaatgagtaa
     2041 agaagagtta agcagaaaat ctgttcaaac attgtttaag aaccaaggag tgtacaatgg
     2101 actattagga gtgggactac tttatggagc ttatctttct tccgcatcta aagaaatcac
     2161 aagtatgcta ttaatatcta ttttttttgt tgcattatat ggcagtttga ctagcgatcg
     2221 ttggatttta ctcaaacaag gtgggctagc tctgttggca ttgctttcta ttttcttata
     2281 aatatagaaa aaataagaat ttatctaaca ggaaactttg tgaaaaatat tactttatct
     2341 catgaaatat gatattctaa tgaatggtat tttagaaaat ttcatgaaaa aggagttaga
     2401 taggtgttca aaaattatgt ttcctcacta aaaaaagaat tttcaggtta tgatctttca
     2461 agatttcaga aagatctttt ggctgggatc acagtagctg ctgtagcatt gcctttagcc
     2521 ttggcttttg gggtatcaag cggagctgat gcggccgcag gtttgattac ggctatcatt
     2581 gccggtatcg ttattggcgg tttatcaggc ggtttttatc aaatatcagg tccgacaggt
     2641 gcgatggcag ctatcttgat gtctattgcg gcaacaaagg gaatgcaagg tgtattgctt
     2701 gctacttttt tagcgggtct atttttactt gcagcaggac ttttccgatt aggaacactg
     2761 acttctttta tcccagcacc cgtaattacg gggtttactt caggtattgc tatcattatt
     2821 gcattaggtc agatcgataa tttatttggt gttcactcgg aaggtacaaa cgttattgaa
     2881 aaactaagta gttatcaaac gataggattt ccgataaaca cagcctcttt gatcatggga
     2941 gggctagtca ttttagggat gtttatctat ccaaaaaaat gggcgaaacg aatgcctagt
     3001 tcgctgctgg ctatcatttt ggcaacagca gttatgctat tgttccattt accagtagca
     3061 actgtgggta agattccaca aacattgatc agtagtaatc gtttgaatat gggagatttt
     3121 agcttttctg ccttgcaaca agtggcagtt ccagctatta gcattgcttt attaggaatg
     3181 attgaaagct tgctatgtgg tgcttcggct ggccgaatgg caaataagcc cttagatagc
     3241 aatcaagaat taatggcgca aggaattgga aatctgttgc ttcctttctt tggcgggatt
     3301 cctgctactg cggccatcgc ccgtacaagc gtggccatca aatctggtgc acaaactaga
     3361 ttagctggta tgatacatgc agttgtctta ctgctctcca tgcttgtttt tgcgccagtg
     3421 atgtcacaga ttccaatgcc agcacttgca ggtgttttga ttgtgacggc ttggcgtatg
     3481 aatgaatggg aaacgatcaa ggagctattt actaataaat attggtcagc agttctattg
     3541 tttatcctta cgatgatttg tacagttatc tttgatttaa gtattgctat cgtgatcggg
     3601 gtgatcggcg gctgtgtatt ttttgttgtc aaaagcgctg ctatcacgat ttcattagaa
     3661 acgatcgatt ggcaaagaat ggggctgcca gaaacggaaa agctgaataa ctggacagta
     3721 gtgtatatta gtggtccact gttttttatg tcttcagaaa aactcaaaaa taaattggaa
     3781 gaactaaatc atcacgatgg tgtcattttc tccatgcgag gcgtaccaag tattgatttg
     3841 acggcacgtg cagtttttga tgaatttcaa gaaaaagcag agcaaagagg acaaacgatt
     3901 atctatactt ccttgcagcc agaagtagaa aaacaacttt ctcacttgtg ggaacataca
     3961 ttcacgcatc aacatcaaac agttgctcat gcgttgacag ctttacatgc gaaatatact
     4021 gcaattagcc attaaaacaa tgaaaaagaa cagacaagac tgtaaactga agattttatg
     4081 gtcttgtctg ttctttttaa tattgctgta aaagtgaagc tagtacttgc cagtcttgat
     4141 ggataacagg tacaagggag cgattgtaga aaatagctgc tggatgaaaa agtggaagaa
     4201 ttcgatagtt tttttctgac cattcgtagg catccttttg ttctgttaat tgtaagacag
     4261 gccctgtata gagttgacca tgatttttgg taaccgtata ttctttgcca agtaaacgtt
     4321 gaagaccaat gtttccaagt gtcgcaatta attttggctg gaccttcatc agttcgtaat
     4381 ccaatattgg tgcatgcgca aatacttcgg aacgagtagg tgtccgattt ggataaacag
     4441 tttctgactg ttgtgtccgt ttattgatcc gatgagtcac gcgatacggg cggctcctta
     4501 cagcactggt gatatacacc tcttctcgtg ttaagccagt tgaagaaagt gcctgcatca
     4561 attcttttcc agcttgaccg ctaaaaggaa catgcgtatc aatctctgtt ttccctgggg
     4621 cttctcccac gagcatcagt ttaggatgaa gaggtccttg cccagcgata aatccttcta
     4681 caggacggcc gttcattctt aaagaagctt cttgtatcag ttcttttggg tattccattt
     4741 atgctcctcc tttttctttt attataagaa taaaagaaac acaatgcgac tattttattt
     4801 ggtagaactg tgaggataga aaagcagaag tattgcttga atttttgttt tactttttga
     4861 ttttaaattt agttgactaa cggttgctgt tctggtaagc tagtactgtt gtaattgtgg
     4921 actccacagc tacaaccgct cagaacaagt aataagcaca gcatatgctg tcacttcgga
     4981 tggcgagtct aagtatatca gaaggaggtg ctttatagca tgtacgcaat tattaaaact
     5041 ggtggtaaac aagtaaaagt tgaagtggga caagcaatct atgttgaaaa acttgacgtt
     5101 gaagcaggag aaaaagttgt ttttgacgaa gttatcttag taggcggaga atctacaaaa
     5161 gtaggcgctc caactgttgc aggtgcaact gttgaaggta ctgttgaaaa acacggcaaa
     5221 caaaagaaag tcgttacttt caaatacaaa cctaaaaaac attcacaccg taaacaaggt
     5281 catcgtcaac catatacaaa agtggttatc gacgcgatca acgcttaata gcgtttgaga
     5341 aagaaggcct atatatgatc aaaggaacat ttaagcgaaa tgacgcaggt cagatcgttt
     5401 cattcacttt gaccgggcat gctgatgctg gtccttatgg aagtgatatc gtttgtgcgg
     5461 gtgtttctgc gttagcgatc agcactgtca acgggattgc ctcattagcc ggttttgagc
     5521 cgattgttga aatgaatgaa gaagaaggcg gttatcttta taccgaagtc acttcaggaa
     5581 tgacacaaga acaaaacaat attgctgaga ttctattaga aaatctttta ttaggattac
     5641 aatcgataga agctgaaaac tcagaatata tccaaattaa aacaattaat gataaatagg
     5701 aggtgcagac catgttgtta tctatgaatc tacaattatt tgcccacaaa aaaggtggcg
     5761 gttctacatc taacggccgt gactcagaat caaaacgtct aggtgctaaa cgtgctgatg
     5821 gacaaacagt tacaggtgga tcaattcttt accgtcaacg cggtacaaaa atctatccag
     5881 gtgtaaacgt gggtatcggt ggagacgaca ctttatttgc aaaagttgat ggcgtagtac
     5941 gtttcgaacg taaaggccgt aacaaaaaac aagtgtctgt ttatccagta gctcaagaag
     6001 cttaattgaa tcttatgatt aaagaggttg tgacaagact tttgtcacag ccttttttgt
     6061 ttataaaaat aaaagttgta gtggaggcta cgaagtttac tttgtctttt tacacttaat
     6121 atttttttgc caatttggaa tcaggatgtt ttgataacta tcgttttcaa ttgatggaag
     6181 ctatattgtt tgctatcagt gaaataatta tatggtaaac agtgttcttt ttcttgtttc
     6241 cattataaaa atcgtatcgg gatttttcgt tcttttaaat tgaacatttc tatctatccg
     6301 cgtgttatca acaaaacaaa atcaggtaaa tagttgacga aacagaaata aatacatagt
     6361 attatgtttt gaagcctaag tacaatagtc tatagaaaag gagcgatacc attggcaaaa
     6421 cgcattgacc gaatttatat gtatgtcaaa gaaaaaaccg ctcatttaac aaaggcagaa
     6481 tatgatcaag gagtaacaac acaggaaatt gctgaagtgt tagggattca gcgaacaaat
     6541 agcagtaaag atctgaatca gctagtgcgc gaaggaaaac tgataaagac agacggacga
     6601 ccagtacgtt acatatatca aacactcgtt acgcacgtaa agccagttac aaaacatgta
     6661 gtcagttaca aagaaacgcc ggtctctgaa gaaaaaaata cactccaaat agatactaag
     6721 gatatctttg caaaaatcat tggagctaac ggcagcatga aaaattcagt ggaacaggca
     6781 aaagctgcga ttttgtatcc gccaagagga ttaaattgtt tgattacagg acctaccgga
     6841 tcaggaaaga cttattttgc acacgcgatg tatcattttg caaaagcgca tcatgtaata
     6901 gatgaggaaa cggaactgat tgttttcaat tgtgctgatt atgccaataa ccctgagctt
     6961 ttgatgagtc atttatttgg gtacgcaaaa ggtgctttta caggcgcaga tgaagaaaaa
     7021 atggggatta tcgatcaagc ggatggcggt atgctgtttt tggatgagat tcaccggttg
     7081 ccgcctgaag gtcaagaaat gatcttttac tttatggatc atggaactta cagtcgtctt
     7141 ggggaaacaa cgaaatccca tgaagcaaat gtacggatcg taggtgcgac cacagaagac
     7201 ccaggttctt ctctattaga aacttttgtt cggcgtatcc caatcaatat caaacttccg
     7261 gcttttgaaa aacgtcctgc aaatgagaaa gtcgatttag taaaaatcat gattgcccac
     7321 gaagcaaacc gcacgcagcg aaagatttct ttgacagaag atgtagcaaa agcattgatc
     7381 ggcagtgtga cctatggaaa tataggacaa ttgaagtcaa atatccagtt agtatgtgca
     7441 cgaggatttc taaatcatat gcaatcaccc gaaatctcga tcacaatcga tgatttgaca
     7501 gaagggattc gtagcggatt gattcaatta gcaagcaacc gcagtgcaat ggcagagctt
     7561 tctaaaatat tggagccaaa gataactgta gcaccaaatg atacgatcat gaaaatccaa
     7621 tcggactcct atgagttacc ttataatcta tatgatatca tcggagataa agcagcttta
     7681 ctgaagtcgg atggtttgga tcaagaagca atcaatcatt tcatttctac agatatcaac
     7741 atccatctga agtctttcta taaagaccat ggtttttctt ttaatgcaga cagcaaatta
     7801 gcagaatttg tagatccgaa aatcatagaa gtgaccaatc agatttatgt aatggtcaaa
     7861 agatctttac catatgagtt tcagcaaaac tttatttatg ctatgagctt gcatatcagt
     7921 tcttttttga aaaaaatcaa cttaggagaa gaacggcata cgaatgataa catccgtgag
     7981 atggcttttg attttccaga tgaatatgct gttgcgaaag aagtgcgtag ttatatcgaa
     8041 acgtattttc aagtggagat tccagaaagt gaagactatt acttgaccgt tctactcgtc
     8101 tcgttacggg caaatcaagc aagcggcaga atcggtgtag tcgttgcagc tcacggaaac
     8161 agcacagcaa gcagtatggt ccaagttgtc caacagttgt tagatgcaga gaacgtacga
     8221 gcagtagaca tgcctttgga tatggatccg aaaacagcat tggaccgaat tgaacgcaat
     8281 gtacaagaag tagatgaagg aagcggggtc atactcttag tagatatggg gtcccttgcg
     8341 tcgtttaatt cgcaaattca aagagacact ggaattccag tacgtactgt tgatatggta
     8401 acaacttctc tagttttgga aactgtacga aaggtctcag tattagggac agatctagat
     8461 atgttgcatg aatcattgaa aaattttcga ggttacgcag aaatatcttc tgtagaatct
     8521 gttcaggaag caaaacctat tcataagaaa aaagctgttt tggctatctg cgcatctggc
     8581 gaaggtacag cacaaagaat caaagaattg atcgaacgag cggtaaacaa gcgtcaagaa
     8641 acagaattag aagttctagc tttgtcgatt gtggaagtga aagaagaact gccagttatc
     8701 caaaaagaat atcagatcat agctactact gggattactg atttgaatat ccaagcccca
     8761 tttattcctt tagaacgttt tattgatcaa aatatcgaag tcattttgga tcagttgctg
     8821 atggaatcag aattggagga aactgaattc atctccctag atgaagagtc agctaaaaat
     8881 acgtgtgttg agtttatctc tgataacttc atctttatta atggtagtaa gttaatcgat
     8941 ccaatgtggc aattttctac acaaatctct cagacgacgg gaatcggcga tgaagaatat
     9001 ggattcaaaa tcaacctagt gatgcacaca gcaggaatga tcgaacgtat tattcggaat
     9061 gaaccattga ctgtggaaga aaacgaattg acaaatacaa cgaatgatcc tctttatagc
     9121 caattggcag cctcagttgt tctgttggaa gatcagataa aagtaaaagt gccgatagaa
     9181 gaaatgtatt atctgctacg tctagtccat aatcagttag ataaaaaaga atacactgtg
     9241 ccatgaatac acaaaaacga tacactttta tagtgtatcg tttttgtgta ttggttaaga
     9301 atcctttaaa ttcaattagt gtatcgtaaa aaagttggca cgctttttgc ttatagtagt
     9361 gtgtgctagg aggtggttgt tcacacagaa atgatagggg tttctgtgaa gtagtgagga
     9421 agaaggagtt ttgcagatgg atattcgatt ggcacgtata gatgatcgtt taatccacgg
     9481 gcaagtcgca acagtttggt cgaagcaaac aggtgttgaa cgtatcattg tgatcagcga
     9541 tgtagtcgct caagataaat tgagaaagtt tctactaaaa gaagctgcac cgccggggat
     9601 cacagcaaat gtaataaccg tgcagaaaat gattcaaatc tatcatagtg gattattatc
     9661 acgagcaaaa gtgatgcttt tattcaccaa tccccaagat gttgaagcgg ttgtccgctg
     9721 cggtgtacag ttgaagtcgt tgaatatcgg cggtatgagt tttactgaag gcaaatcaat
     9781 gattaccaat tttgtttcag taaatcaaca agatattgat tcctttcgtt acttgaacag
     9841 tcaagaagtg gagttagaga ttcgaaaagt acctgctgac aggaaaatcc ttttgatgga
     9901 actactagcg aaaatcaaaa aggtgtagca agaatggttg ttggaaaagg tcaaataatt
     9961 ctgttgtaat ggttagcaat tattaatatt tttgaatttg ttacaacaaa agtaaatagg
    10021 aggtataaat atggtaggga ttatcctagc aagtcatgga caatttgctg aaggaatctt
    10081 acaatccggt tcaatgatct ttggcgaaca agaaaacgtc aaagccgtga ttttgaaacc
    10141 aagtgaaggt ccagatgact tgagagccaa attggaagaa gcggttgctt cttttgataa
    10201 tcaagacgaa gtgttattct tagttgattt atggggcgga acaccgttca atcaatcaaa
    10261 cacattattc gaagagcaca aagataaatg ggcaatcgtc agcggtttga atttaccaat
    10321 gttgattgaa gcctacgcat cacgcttttc aatggaatct gctcatgaaa ttgcagcaca
    10381 catcattgaa acagcaaaag acggtgtaaa agtaaaaccg gaagaattag aaccggcaga
    10441 agcaccgaaa gcagcagttg aagacgcaca acctaaagga gcacttccag aaggaactgt
    10501 ggtcggagac ggaaaaatca aatatgtatt agcacgtgtt gactcacgtc tgttacatgg
    10561 acaagttgcg acagcttgga caaaagcagt gcaaccaaac cgtattatcg ttgtttcaga
    10621 tgcagtatca aaggatgatc tgcgtaagag actaatcgaa caagctgctc ctccaggtgt
    10681 aaaagcaaat gttattccta ttagcaaaat gatcgaagta gcaaaagacc ctcgttttgg
    10741 aaataccaaa gcattgcttt tatttgaaaa tcctgaagac gtattgacag ctgttgaagg
    10801 tggcgtagac atcaaagaat taaatgttgg gtcaatggcg cattcggtcg gtaaagtcgt
    10861 tgtaagtaaa gtattgtcta tgggacaaga agacgttgaa gcgtttgaga agcttgaaca
    10921 aaaaggtgtg aaattcgatg tacgtaaagt acctaatgat tcacgcgata acatggatga
    10981 tattttgaaa aaagcaaaag ctgaattagc aaaagcgtaa taaataatat taaaatttat
    11041 aggaggctta tcatgtctat tatttcaata attttagtcg tattagttgc ctttctagct
    11101 ggtatggaag gtattcttga tgaatttcaa tttcatcaac cactagtggc atgtacattg
    11161 atcggtttag ttaccggtaa cctcgaagct ggtatcgtac ttggcggtac gcttcagatg
    11221 atcgctttag gttgggcaaa tatcggtgcc gctgtagcac cagatgctgc attagcatca
    11281 gtagcatcag caattatttt agttttaggt ggacaaggtg ttaaaggtgt tccttctgcg
    11341 atcgcgattg ccgttccttt ggctgttgca gggttgttct taacaatgat cgtacgtaca
    11401 atcgctgttc caatcgttca tttgatggat gcggctgctg aagaaggaaa catccgtaag
    11461 gttgaaatgt ggcacatcat tgctgtatgt ttacaagggg ttcgtatcgc ggtcccagca
    11521 gcagcgttgc tatttatccc agcacaaact gtgcaatcat tccttgaatc aatgccagca
    11581 tggttaacag atggtatggc tatcggtggg ggaatggtcg tagctgtagg ttacgctttg
    11641 gtaatcaaca tgatggctac aaaagaagta tggccattct tcgttattgg tttcgttgta
    11701 gctgcaattt ctcaattaac attgattgct ctaggtgctt taggtgttgc attagctctt
    11761 atttacttga acctttctaa aatgggtggt tcatcaaata atggaggcgg aagcaactct
    11821 ggtgaccctt taggcgacat tttgaatgac tattaatctt gaaggaggaa aataaaatgg
    11881 cagaagaaaa aatcaaatta tcaaaaaaag atcgtttagc tgttgcgtgg cgctctacct
    11941 tcattcaagg ttcatggaac tacgaacgta tgcaaaacgg tggctgggca ttttctatga
    12001 ttccagcaat caaaaaatta tacaaaacaa aagaagaacg ttctgcagca ttaaaacgtc
    12061 acttggaatt ctttaataca cacccataca ttgcttctcc aattttaggg gttacattgg
    12121 ctctggaaga agaacgtgcg aatggcgcgc ctgttgatga tgtagcgatt caaggggtta
    12181 aagtaggtat gatgggtcct ttagctggtg ttggtgaccc agtgttctgg tttacagtac
    12241 gtccaatgct aggtgcacta ggtgcttcat tagctatggg aggaaatatc cttggaccaa
    12301 tcatcttctt cttaggttgg aacattattc gttgggcatt catgtggtat acacaagaat
    12361 ttggctacaa agctggttct aaaattactg atgacctttc tggcggattg ttacaagacg
    12421 taacaaaagg tgcttcaatt ttagggatgt tcgtattagc tgcccttgta caaagatggg
    12481 tatcaatcaa attcttacca gttgtatcaa ctgtaaaatt agataaaggt gcttacatcg
    12541 aatgggataa attgccttca ggtggtgaag gaatcaagag tgcatttgaa caagttaata
    12601 atggcttagc cctttctcct gaaaaggtaa caacattaca agacaactta gaccaattga
    12661 ttcctggttt agctgcatta ggattaacat tgttatgtat gtggttgctt aagaaaaagg
    12721 tttctccaat cgttatcatc cttggtttat tcgtagtcgg tgttgttgga cacgtcatcg
    12781 gacttttata atccataaaa atatagctcg ggcgttgtcc cgagcttttt ttatatagta
    12841 tactgttcat aaacaaatat ctggagggag aaaatggttc aatcaatcaa tacaaaagta
    12901 gacttggtaa ttgatgctac ttcgtttacg ggtctgacag attacgggca aatcatgata
    12961 ggtgataatg gttttgagtt ttaccatgcg cgagatagac gcaaatatat ccaaattcca
    13021 tgggaagaag tggattatgt actagcatca gtcatgctaa agggaaaatg gattcctcga
    13081 tatgccattc aaacgaaaaa aaatggtaca tacacatttt cttccaaaga acctaaaaaa
    13141 gtgcttcgtg cgatcagaga atatgtagat gaagatagaa tggtacaatc attgagtttc
    13201 tttgatgtag tgaaacgtgg tattcgtagt atgtttaaac gcaagtaaaa aataaagaaa
    13261 acttctaaga ggaggttttc tttatttttt tactttgcag aagaaaatat gaaatagcaa
    13321 aaccaattat ttgtcccctt taaatcccat atgctaaaat tgattagcaa agaaggaggt
    13381 tatattatga gttggttatg gacattgatt gttggagccg tgatcggagc aattgcaggt
    13441 gcgatcacca gtaaaggaaa ttcgatgggc tgtgtaacga atatcattgc tggactagtc
    13501 ggttcaagta ttggacaagc gattttcggc gaatggggac caaaagctgc aggtatggca
    13561 cttattccat ctattttagg cgcagtgatt ttggtagcag ttgtctcatt tttctttgga
    13621 aaacgatcat aagtttttac aaaatagtaa actaaaagga gttgcagcga gactgtgact
    13681 cctttttggc tttatttatt taaaataaaa agaagttttc cggaaatttt tatttaactg
    13741 tcgtgtacga agaaaagttt gctataatgg aaaaaagcgt gcaaagaaag agggagtttc
    13801 attatgatgc tacgtgtaga aaaattgcgc aaaaaaatgc aagaagaaaa tttggattca
    13861 tttttggtta ccagtccgta caatcttcgt tacttaacga actttacagg tacgacagga
    13921 ttagctgtta ttactttgga aaaagcattt tttattactg attttcgtta taccgagcag
    13981 gcggcagcgc aggctcaagg gtttgaaatt attaaaaatg tcggacctat ttttgatgag
    14041 gtagcaaact tagtacgcaa agaagaactg cactcattag cttttgagga aacaacagtc
    14101 tcatttttag aatattcggt tttagaagaa atcatcgatg cggaattagt acctgtttca
    14161 cagatgatcg aagaactcag agaagtgaaa gatgaagaag aaatcgctat tatcgaaaaa
    14221 gcatgtcata ttgcggatat ggcatatgat cacatcctga aaatgatcca accgggcatg
    14281 actgagattg aagtcgcaaa tcaactagac ttttatatgc gttctcttgg cgcttcaggt
    14341 gtttcttttg atacgatcgt tgctagcgga ttgcgttcgg ctatgccgca tggtgtagca
    14401 agcaaaaaaa tcatcgaaca aggtgacttg atcacattgg actttggttg ctattacgaa
    14461 gggtacgttt ccgatatgac acgtacattc gctattggtg atcctgggga aaaattaaaa
    14521 gaaatttatc aaatagtctt agatgctcaa ttagcagtta ttgatgctgc aaaaccagga
    14581 atgactggtg tccaattaga tgcagttgct agagattata tttcaaaaca tggttacggg
    14641 gaagcatttg gacattctac aggacatggg atcggtttag agatccacga aggaccaaac
    14701 gtttcttctc gtgcagaaaa gcaatttgta ccaggaaatg tgatcactga tgaaccaggg
    14761 atctacttac caggaatcgg tggagtacgt attgaggatg acttgttgat cacagaagat
    14821 ggaaatcgtg tgttgactcg ttcaccaaaa gaattgatca tcttatagtt tcatcaatag
    14881 aagaagtaag cgtaaaaatc agtgttcaca gactttgtta tagaaaatca ctcgatttaa
    14941 tgatacacta tagaagaact tttaagctgt aagagataat aaagttgggt gttttctcca
    15001 attcatagca gtttgcagct gatcggcggc agttttctta ccacgtgatc actatgccgc
    15061 atcatagaga attcggatgt atctcgtgct taggcaaagg gaggaataaa taaatggcag
    15121 atgaaaaaaa tttgatttta aacgcaaacc aagatttagg agagatcgtg atcgctcctg
    15181 aagtcatcga agtaattatc gggattgcag cttctaaagt agaaggcgta tacggaatgc
    15241 gcggaacttt tgccagcaat gtcactgaat tattaggacg tgcagctcac ggaaaaggtg
    15301 tttacttagc aaacgaggaa gatggcttga aagtagatct ttactgttat ctagaatatg
    15361 gtgtttcagt tcctaaagta gcaatggaaa tgcaggaacg ggtcagccag caagtccttt
    15421 tcatgacgga tatcgattta tctgaagtca atatccatgt agtagctgtt gttcctgaaa
    15481 aattagaaca accaacaatc gaagaacttt ttgaagacga agaggaagag aatgaataaa
    15541 gaattatcaa gacacgagat tcgggaaatg gcattgcagg ctttatttcc attagacttc
    15601 aatgcagacc taacaaaaga agatgcgatt ttcaacgcaa tcgaattaga ccaccgcgac
    15661 atgatcaacg aagatgaatc agagtttgtt cctgtttatc ttgatacgct agttggcggt
    15721 gtctgtgcaa aaaaagatga attggataaa gtgattgaaa aacatttaaa aaacaattgg
    15781 cacatcaatc ggatctcaaa aatggatttg gtgatcttgc gtatcgcgat ttttgaaatg
    15841 acttatgtga cagatgtacc agcggctgtt gctttaaatg aagcaatcga gttggcaaaa
    15901 acatttagtg atgaccgttc acgtaaattc gtcaacggtg ttttatccaa tgtattgaaa
    15961 gaattagaag ctggggcgta agtcctagct tttttctatg aatcatcata aacctgttat
    16021 aaatcgggaa aattctatgg tagaatgaaa acacaaaaaa tatctgggag tggttagatg
    16081 gcagaattga ttaacggaaa agaattagct gaaaaaatgc aggcagagat tgctgaaaaa
    16141 atccaaaaat tgaaagataa tggtatccat cccggattag tcgttttatt ggtaggggaa
    16201 aatccagcca gccaaatcta tgtaagaaat aaagaacgtg tagcaaaaga aatcggaatc
    16261 cattcattgg tagagcgtta tcctgagacg atttctgaag acgaactact tgcagaaatt
    16321 gaaaaataca atcaagatcc tagttttcat ggtatattgg tccaattacc attgccaaag
    16381 catatagatg aagaaaagat cctgttagct atccgccctg aaaaagacgt agacggtttt
    16441 catccgatga atttaggcag actccttgcg ggtaaaccgg acaagattcc ttgtactcca
    16501 tacggcatca tgaaaatgtt tgaagcttat tctatcgatc cggcgggtaa acgagcagta
    16561 gttatcggac gaagcaatat cgtcggtaaa ccgatggctc agctactctt gatggcagat
    16621 gcaactgtta ctgttgctca ttctaaaacc gaaaatctgg ctgaactggc acaagaagca
    16681 gatattttag tcgtagcaat aggacgtgga cattttgtca caaaagaatt cgtaaaacca
    16741 ggtgctgtag tgatagatgt agggatgaat cgtgatgcta acggtaagct gatcggtgat
    16801 gtgaaatttg atgaagtgga gcctattgca ggctggatca ctcctgtgcc aaagggtgta
    16861 gggccaatga ctatcacgat gctgatgtat caaaccgtaa aatctgccga agcttttgga
    16921 gcaggtgaat agatggcaca agaatattta actgtcacta cattaacgaa gtatttgaaa
    16981 agaaaatttg atgccgatcc ttatttagaa cgcgtatatt taacaggcga aatatcgaat
    17041 ttccgtttac gtgccaatgc ccatcaatat ttcagcttaa aagatgacca tgcaaaaata
    17101 tctgctatta tgttcaaagg agcattccaa aaattacgat tccagccaaa agaaggaatg
    17161 aaagtccttg tcgttgggcg tatttcatta tatgaagcgg gaggttctta tcaaatctat
    17221 gtagagcaca tggagcctga tggaataggt gctttatatc aagaattgga agaacgaaaa
    17281 gaaaagctga tgaaggaagg cctttttcaa gggcctaaaa aaatgctgcc acgtttccca
    17341 aagcgaatcg cggtccttac cagtccaagc ggagcggtta tccgcgatat catcacgacg
    17401 gtgaaaagaa gatatccaat tgtccaattg gttttgttcc caacagttgt acaaggaaac
    17461 caagcagcgg acaatgttgt caggaatatc cagagagtag aagagatggg caatttcgat
    17521 acaatgatca tcggtcgtgg gggtggttcg attgaggatt tatggccgtt taatgaagag
    17581 cgggttgctc gggcgattta tcaagctaat acgccggtga tttcatctgt aggacatgaa
    17641 acagatacca cgatcgctga tttagtagca gatgttcggg ctgccactcc aacagcagct
    17701 gcagaattag ctgttcctgt tttgtcagaa gagttgatgc gcattcaaga acggcaagct
    17761 agattgcagc aggcgtttgt acgccagatc cagcgcaaac aagaacggtt cgagcgtgct
    17821 cgacgttcct atgtatttcg gcagcctgaa agattgtatg aaggtcagac catcaaatta
    17881 gaccagttga aacagcgatt atatcaagct acacagcaaa tttatcatgc gaaagaaaaa
    17941 caaacgatcg cattaagcca ccaattggaa caagtggctc cgctttatcg agttaaagca
    18001 gctcgccaag aaactgctta tctagaaaaa cgcttagaag agaaaatcca acaatatatg
    18061 aaacaacagc agcagcgttt tgctcaagct gttcaatctc ttgatttgtt gagtccattg
    18121 aaaatcatgg gaagaggcta cagctatacg acgttaaatg atcaagtagt aaaaagtgtc
    18181 accgagattc atcaagggga tttgctgacg atccattatc aagatggaat cgttaaagga
    18241 caagtacaag agatagacaa aggagaataa aaatatggaa aatccaactt ttgaagagtc
    18301 actgcaagaa ttagaaaaaa ttgtcatgca gttagaacaa ggagacgttc cattagaatc
    18361 agctctagat tcgtttaaac gaggaatgga attaagcaaa cattgccaag atacattagc
    18421 taaagcagaa gagaccttga cgaaaatgat gacagaatca aatgaagaag ttgattttga
    18481 cggaaacgag gaaagctgag atgagattaa ctgacttttc agctattcat ctacctgctg
    18541 tagaaaaaga aatcttgtct tttttagatg agcatacaac ggatagagga ctctatgatg
    18601 cgatgagtta ttcagtgaaa gcaggcggga aaagaatacg tcccttatta cttttaacgg
    18661 ccgttgcttc ctttgatgag cctattgatg ttcccgtcta tcaagtagca gctgctttag
    18721 aaatggtgca tacgtattcc ttgatccacg atgatttgcc tgcgatggac aatgatgatc
    18781 ttcgtagagg aaaacctacc aatcataaag tattcggcga agcattagct atattggccg
    18841 gagatggttt gcttacagga gcattccagc taatcagtat ggcacactta ggtaattctc
    18901 ctaagcttct gctgcttcag caattagctg tttgtgcggg atcacaaggt atggttgctg
    18961 ggcaagctgc agatatcgag ggcgaatcga aaaagctttc tttagaagaa ttagcattta
    19021 ttcatgaaag aaagacaggg catttgattc gttatgcctt gctggctggt ggcatattag
    19081 cgaagcaacc tgaagaaatc ttattgctac ttcaacgatt agccgaacat ttaggtttag
    19141 cttttcagat aagagatgat ctactcgatg tgattggtac aacgaaaaca ttagggaaaa
    19201 cagctggtaa agatgaacgt atggaaaaaa atacttaccc gcgtttatta ggcctagaaa
    19261 agacacgtga ggcgttggag attgagctgt tatccgccaa caagatcata gacaaattag
    19321 aagaggacgt tttttctttt gatggcgggc tgatgcgcca gatgatcaag caatttgatg
    19381 tggagaaatg agggaaagaa tggaaaaaga acgcgtagat gtattagcgg tcaaacaagg
    19441 attatttgaa acaagagaac aagccaaacg ttcagtgatg gctggtttga tttacaacga
    19501 aaaaaacgag cggtttgata aaccgggaga aaaaattcca gtgagtagtg aattaagagt
    19561 caaaggtaaa aaacttcctt atgtttctcg agggggatta aaattagaaa aagcactcaa
    19621 agcatttgat ctatctgttg aaggaaaaac attgttagat attggtgcat ccactggcgg
    19681 atttactgat gcagctttgc aaaatggcgc aaaaatgagt tatgctttag acgttggcta
    19741 caatcagcta gcttggaaaa taagacaaga tcctagagtc gtcgtaatgg aaagagtgaa
    19801 ttttcgttat gcaaaacccg aagactttac cttaggaatt ccagaagttg ctgttatcga
    19861 tgtttctttt atttcactaa aactgatgct gcctccatta catgctattt taaaagaaga
    19921 gggagaagtg attgctttaa tcaaacccca attcgaagca ggcagagaag ctgtagggaa
    19981 aaacggaatt gttcgtgacc ctcagaccca tcaaaaagtt ttagaagaca ttttgtcatt
    20041 tgcggcacaa ggtggatatg atgtgctgga attaagttat tctccgatca cggggggaga
    20101 aggaaatatc gagttcttgg ctcacttgag gaaagtaccc gaaagcggaa caatcaattc
    20161 agctattaat atggcagaag tggtttcaaa tgcacacgaa cagtttgacc ataaataaat
    20221 tgtaactgtg aaaatcaagt gagaagtatg cggattcttc tttattatac caaggaaata
    20281 agctatcatt gattaagaag aatatgctaa ggagcgaaga cgtaatgaga aaaaaagatc
    20341 ggcatcgttt gattactcga ttgttgagtg agcaagatat ccgaaagcag gaagaatttg
    20401 ttgaaatcct gaaaagcaaa ggaatatctg ttactcaagc tacgatttcc agagatataa
    20461 aggaactgaa actgatcaag gtaccggctt cagatggcgg ctaccgttat agcataccag
    20521 ctgaaacaaa cgaagatgta aatactaaat tggaaaaact attaaaagac gctttcgtgg
    20581 cagttgacca aatggaaaaa tttgttattt tgaaaacatt gccgggaaat gcttcggcag
    20641 cagcaaattt gattgataaa cgttacaaga aagaattatt ttcgattatc aatgatgacg
    20701 ataatgtttt gatgattaca cgaactgaag accaggcagt tttattgaag aaagacttcc
    20761 tgcattacct ttaagttgag gagcgaatgt gatgctacaa gaaatcagta ttactaactt
    20821 tgcgattatt ccggaattgc ggctttcttt tcacgaagga atgaccgcgc tgaccggaga
    20881 gactggtgcg ggtaaatcca ttattatcga tgctttaggt ctgttagcag gaggaagagg
    20941 ttcaagcgat tatatacgtc aaggagcaga aaaatgtgtg cttgaagggt tgtttgaact
    21001 accaaaacaa gaaggattct ctgaactgat ggttgaatta gggatcgaaa cagatgagga
    21061 caatctgatt gttcgacggg atatgtcctt aactggaaaa aatgtttgtc gagtgaatgg
    21121 tcatattatc acattagcta atctgagaag aatcggcagt tatttagtag atatccaagg
    21181 acaaaacgaa catcaggaac tgctccagcc tgaatcacat cttgctttat tggatcgttt
    21241 tggagatgta gctttccaac agaagaaaaa gagttatcag caagaatata tctcttatcg
    21301 tgagctggaa aaaagggtac gaaaagtgca gcaaaatgaa aaatcctatg ttcagagaat
    21361 agatatgctc catttccagc aagaagaaat agctgctgct gatttgcaag tcggagaaga
    21421 agagaaatta aaagaagaaa gagaaaaact tagcaattat caaaaaattg ctgatggatt
    21481 agctgcaggt tacggagcac tgacgaataa tgaacaaaac agtgtagatg gtgttggctt
    21541 ggccgtttca gaactgcaag ggatcgccca tctagatgtg gagtatgaag caatttatga
    21601 aaatatccaa agtgcttact atttgctgca agatgcaatc ggcgatatga gtcgtcagat
    21661 tgatttattg gagttagatg agagtcgttt agaagaagtt acgcaacgtt tagaattaat
    21721 ccgacagttg aaacgtaaat acggcgaatc cattgagtca atattggctt actatgacga
    21781 aatcaccgaa gagctagctt cttctgattt ttcagaaggt caattggaca aaatgaaaga
    21841 ggaactagag caaaaagagc ttctacttca gcaacaagcc gcagatcttc atgaagcgcg
    21901 taaaaaaatc gcaaaagaat tggaaaaatc cattctgcat gaattgaaaa gtctgtatat
    21961 ggaaaacact gagtttgagg tacgtttctt aaaagaagaa aatagacaat tgaatcatga
    22021 tggatttgac caaatcgaat tttacattac gaccaatcca ggagagccac tgaagccttt
    22081 ggtgaaagtc gcttctggtg gcgaactgtc tcggatgttg cttgctttga aaactatttt
    22141 ttcttctgag caaggtgtga caagtattat ttttgatgaa gtggatacag gtgtgagcgg
    22201 aagagttgca caggccatcg cagataaaat ttctcaaata tcgaaatact ctcaagtttt
    22261 gtgcatcact catctgcctc aagttgctgc agtcgccgac tatcaatatt atattgtgaa
    22321 agcagtgatt gatggacgta cgcaaacttc tgtttcagag ttgaaaacaa aagaaagaga
    22381 agaagaaatt gcacgtatgc tggctggaag tgagattaca aaactcacac tcgaacatgc
    22441 aaaagaacta ttgaaactcg caaaaaggac ctgagccacc tcgcagtggt tcaggtcctt
    22501 ttcagcagct gatagctata ttttattttt acatgacact cattacagca gtcaatacaa
    22561 cagatccaat cgttaaaatc agcattgtcc aaatgacaaa ttttgttact ttagcaaaag
    22621 tacttgtttg ttttttttca cgcattgttt tcaacctcgt cattaattag ttgattcaag
    22681 tgtacaatct tttttccatt tccacaagag ggctttttta taaaaacttc ccttttttta
    22741 aataataggt agttacgata caaataaata atgttaatag aactagccat ataaaggcat
    22801 catccctttc agcaataggt aacttaatgt tcattccata gatactgccg acgattgtcg
    22861 gtatagttaa aataatcgtt aaagaagtta aaattttcat aatattattc agattattcg
    22921 atacgattgc tgaaaaagtc tgattcattt gttctactag ttttaattga atgcgagaag
    22981 tagtcattgc ctgctctgtt tccaccaata catcacgtaa atgatttttc aaagcatgat
    23041 tttcttggaa aagtttattt tgcgctagtt ctttcaatgc atctaaatta gcagaagtcg
    23101 cttctctgaa ataaacgagg cttttttgga tatccatgat ttgataaagt tgtttgtttt
    23161 cagtggatac ttgaagtttg gcttccaaag tatcgcattg agattgaagc gtagctaaat
    23221 aactgttgta agagagtgtg acctgccaaa tgaattgaaa gaagatattg agattaggcg
    23281 aaatatcact ttttggaaag gtctggctaa agactttctt taaaaacagt ggttcatgat
    23341 tcgtaattgt gataagcttc tttttgggcg tcaaaataat agagagcggg tatgtatcca
    23401 cttgaaaata accgctagga cttgttttag caaaaggata ttgtaacaat aataacgctg
    23461 gctttgtcaa ctttgattga ttgaattctt ctgtacggga attttctgca tcgtccaata
    23521 tggaagtgat gtaatctgac ggaatttcaa attgatgtgt cagctgatga atctcttctt
    23581 gattaggacg ttctacacaa taccagatcg tttggtcagt ttcctttccg gtattacgca
    23641 gtttctcatt atctaatgta aaatatttga tcatgatttt cctcctgttg cctgtttctt
    23701 ttattataac ggatagggaa ttggaataca tggaatgtgt ttgtaagata tagaatataa
    23761 ctgcataaat ttaaataaaa tgaaacagtt gtgtaaagta tatacagtat acttgaagca
    23821 acgataaaaa aggagtttac gttcatgggc acattcacac cacttatctt gttttggtat
    23881 atttacccgg taatcgtttt gtttggttgc caatttcttg taaaattatt ttcgttaaat
    23941 cgtcgtttta aaataaaagc ccctgatttg gcgattcctt ttttgtgggg cggtatgcat
    24001 gcattgtcta gaaatacagg gacaatttct atcttaccgt ttttctttat ttctgttttg
    24061 ctaatgggaa tttgtattgc tgttttccaa gcatattatt atgaagaaat tatttatccg
    24121 cgttatttca agatgacttg gcgtttggtt tttctgttga caatcgtctt gtacgctgtc
    24181 ttgatcattg tgaacatact ttcttaccta tgaaaaaaat agaaaagctg taaaaagtct
    24241 tttaaaaggc tttttgcagc ttgattttta aaaaaatgtt tatgaataaa aaaagttgtt
    24301 cccggatttc ccagaaaagg tggtagaaag tgggggattg tggtagacta attttagtta
    24361 gtggaggaat ggggggcttt caatgttgat gggtgaattc caacataata ttgatgcaaa
    24421 aggacgttta atcgtgccct caaaacttcg tgaagaactt ggcgaaaagt ttgttctgac
    24481 acgcggattg gatggctgtc ttttcggcta tccgatgagc gaatgggaaa atctggaagc
    24541 taaactgaac gaaatgccgt tggctaaaaa agacgcacgt acttttgtgc gattcttcta
    24601 ttcggctgcc actgaatgcg aacttgataa acaaggacga atcaatatac ccagcacgtt
    24661 aagaaattat gcagctttga caaaaggttg cgtagtcatc ggtgtatcta accgaattga
    24721 aatttgggat gagacacgat ggcaagattt ttctgcagca gcagaagaga actttgacga
    24781 aattgctgaa agtatgattg atttcggact ataagaatag gaagaggctg actaatgact
    24841 gaaacatttc aacactatac agtcatgttg aaagaaacgg tagatggatt gaacatcaaa
    24901 ccggatggcg tttatgtaga ctgtacactc ggcggtgccg gacatagtga atatttatta
    24961 agccaactaa gttctaaagg gcatctgtat gcgtttgacc aagatcaaaa agcaatcgat
    25021 cacgccaagc tgcgtttagc ttcttatgtc gaacaaggta tggtcacatt tatcaaagcg
    25081 aacttcagag aattagaaga acgcttgagt gagcatgtag ataaggtaga cggcattttg
    25141 tatgatctag gggtttcctc tcctcagcta gatgaagcag aacgtggatt tagttatcat
    25201 caagatgcac cgttggatat gcgcatggat cagtcagctc ctttatccgc ttatcacgta
    25261 attaatgaat attcctacca tgaattagta aagattttct tccgctatgg cgaggagaaa
    25321 ttttccaaac aaattgctcg tgaaatcgaa cgagtacgaa aaacaaaacc aatccaaaca
    25381 actggtgagc ttgtcgaaat cattaaacaa gtcatcccag ctcccgcaag aagaaaaggc
    25441 gggcatcctg caaaaagaat ttttcaagct gtgcggattg cagtaaatga cgaactcggt
    25501 gccgaagagg cctctttaga acaggcaatc cggttattga aagtcaatgg aagaatcagc
    25561 gtaatcacat tccattcatt ggaagacaga attgtcaaaa gtatgtttaa agaatatagt
    25621 acggtacaag atctaccacc gggacttcct gtcatacctg acgaattcca accagaactg
    25681 aaagtcatta ctcgtaaacc aatccttcct tcagaggaag agctaacaga aaacaatcga
    25741 tcacgcagtg caaagttacg tattgcagaa aaaataagaa tgaaagagga gtagaatgat
    25801 ggcagaatta aagaaaatgc aagattacga atatgattta cctgaattgg tggatcagcc
    25861 ggaacaacaa atgcctgatt cttcttcgaa tcaagaaatc attcttccac aatcaccggc
    25921 aaaacgatta aagcagattt ctgctttgga aaaaattgct gtggcaagcg tggcgattgc
    25981 tattgttgct ttatgtgttt taacagttat gctgcgcaca aatatcagcg gagctgaaaa
    26041 agaaatcacg tcaatacaaa cagaaatcaa tgataaaaag caagaaaaaa cgagtttaga
    26101 acaagaaaaa aacgaacttt ctcgtactga acggattaaa caaatcgcag aagaaaaagg
    26161 attgtctatt aatgacgaca atttaaggaa agtgaagtaa atgagtctta aaaataaaat
    26221 tcggcggttt atggaaaaga aaaatctaaa tccgatgaac aatcgcaaaa aagtaggtat
    26281 cattttattt gctacgagta ttggattgtt ctttttattt gccgtccgtt tctcgtatat
    26341 cgttattgga ggtcatgttg ctggtacctc attagctgaa aaaacaaaac agttgtacca
    26401 aggaagtgaa gtagtaaaag caaaaagagg aacgatttat gatcgtaacg gtgtggcgct
    26461 cgcagaagat gcctcctctt attcaattaa agcgatactc tcaaaaacat atacctctgg
    26521 ggataaaaaa ctgtatgttg aagaaaagaa ttttgataaa atagcagaaa tcctgcataa
    26581 gaatctttct atagacaaaa aagatgcact gaatatttta gaggacggcg caaaaaaaga
    26641 gctgtatcaa gttgaatttg gtagctatgg gaaaaatatc tcccaagaaa ccaagcaaaa
    26701 catcgaagcg gacatgaaaa aagaaggtgt cgctggtctt tattttgtcg accatcaggc
    26761 tcggatgtat ccaaatggcg tcttttcttc tcattttatt ggatacgcag ttccagacaa
    26821 agatgagaat ggcttagttg gtaaactagg tttggagtct gcttacaatg atatcttgag
    26881 cggtaaagat ggtaagatta tttatcaaaa agataatttt caaaatcctc tgccaggtac
    26941 agtggcagaa gaagaaaaag ctgtcgacgg acaagatatc tacacgactt tagacagcag
    27001 actacaaagt tatttagaaa cactgatgga tcaagtaaat gaagaatatc agccagaaga
    27061 attgacagca gtcctgatga aagcaaaaac cggagaaatc ttggcaatgg gacaacgacc
    27121 tactttcaat cctgaaacaa tggaaggatt gactggaaaa gatgctatct ggcgtaattt
    27181 cttggtacaa gatagttatg aaccaggatc aaccatgaaa gttttcacta ctgcagctgc
    27241 tatcgaagaa ggagaattca acgagaatga aaccttccaa tctggaaaaa tccaagtaga
    27301 agatgctacg atcaatgacc atgactttgg tgaaaaaggt gtgctgacca tgcgtcaagc
    27361 tctttcatgg tcaagtaacg tcgggatggt catactggaa cagcgattag gcggacgatg
    27421 gtataactat ttacaaaaac tgggatttgg acaaagtact cattctggat tagatgatga
    27481 agtaaacggt gctttaccta cattaaatat tgtcgaccga gcaatgagtg cttatggaca
    27541 agcggtaggt gtgacgaatt tccaaatgat gaaagctttc acttctattg cgaataacgg
    27601 tacaatgatt caacctcgat atatcagcaa agtggtggat ccccaaactg gagaagagcg
    27661 aacaactcaa acagaagttc tagggcaacc tttttcaaaa gaaacaacag aaaaagtccg
    27721 tgaatatatg agagatgttg tcgaaagcga aaactacggt agtgcttatg gtgtatatag
    27781 tgtacctgga tacaatgtat cggcaaaaaa cggaacagcc caaatcgctt ctgatacagg
    27841 gggatatcaa acaggagata cagcctacct gtattcgatt gtggagatgg ttccttctga
    27901 agatccagac tatgtcttgt atctaacaat gaaacaccct aagacatatg atcgaatggc
    27961 attggctaaa atcgcaaatc cattgatgaa acgggcaatg gattttaaag aaactgaaga
    28021 agattcagat acagaaacaa aaacagaaaa agtatccgta gccgattatc gtaatcttga
    28081 agcggacgtt gcagcagctg atgctcaaaa aagtggactg caacctgtag tgatcggtaa
    28141 tggtaaaaaa gtacaaaaac aatctactgc caacggtgat cagttgatct ctggagaaaa
    28201 actgatccta tatactggtg gtgataagct gatgcctgac gtaacaggat ggtcaaaagc
    28261 agatatcatg aagctgggga aaatcctcgg tattgaagtt agttttgatg gagatggtta
    28321 ttgtgtcaaa caagaacttg ctccatatga aaaaatcact gaagacaaac ttagttttac
    28381 tttagaagaa taagaagaga aataggagag aaaagagcat ggaatggaca caagcattga
    28441 ttcccattgt cagcagttgt gcaatgacaa ttgccgcaat gccgctattt attggttatt
    28501 ttcaaatgaa aaagcaaggt caggcgattc gtgaggaggg accaaagtgg cacaatagca
    28561 aagccggaac tcctacaatg ggcggcttag tgtttctgat cggctctatt ttgacaggaa
    28621 tttgggttgg agcttggcaa aaacagctaa ctcctactct tttcatcttg ctttttgtat
    28681 tagcgttata cggagtaatc ggctttcttg atgattttat aaaaatattc aagaaaagaa
    28741 acatgggact gaactcgaag cagaaattga ttggacagat tctaggggga atcatctttt
    28801 atcttgttta ccgttctgaa ggttatccag gtgtattgaa tttcttcgga ctagatctac
    28861 ctttaggaat catttatggt attttcgctc tcttctggct tgttggattt tcaaatgcag
    28921 taaatctgac agatggcatc gatggattag ttgctggctt aggaagtatc tcttttgcag
    28981 cttacggtct aattgcttgg catcagcatc agtatgatgt attaatcatt tgcttaagtg
    29041 ttttaggagg tttactgggt ttcttcgtct acaacagaaa acctgccaag atttttatgg
    29101 gagatgtcgg ttcattagcg ttgggcggac ttttagcggc aatttctata atgctaaacc
    29161 aagaatggac attgcttttg attggtttga tctatgttat ggaaacagca agtgtgatgc
    29221 ttcaagttac ttcctttaag cttacaggaa aacgaatttt taaaatgtct ccaattcatc
    29281 atcactttga aatgtgcgga tggtcggagt ggaagattga tacagttttt tggttgatca
    29341 gcattgtgac ttcgttgatc acattatgga tcgtttggta ggagatgacg ggaaatgaaa
    29401 aaaatatcaa catatgaaaa taagaaagtg cttgtcttag gactcgcaaa aagcggagta
    29461 agtgcggcca aattattaca tgaattgggc gcacttgtca ccgttaatga tggaaaacct
    29521 tttgacgaaa atccggaagc acaagagctg ctttctttag ggataaaagt aataacaggt
    29581 agtcatccaa tcgaattgtt ggatgaagaa ttttcattga tggtcaaaaa tccagggatt
    29641 ccctacagtc atcctttagt agcaaaagcc caagaaatgg gtattccggt aattacggaa
    29701 gttgagctag cttacgaagt agcggaatgc ccaattattg ggattacagg aacgaacgga
    29761 aaaacaacga caacaacgat gacaggacta ctactgaatg caggagcaga tcaaggaatt
    29821 gcacgtttag cgggtaatat tgggtatcca gcaagtggtg tagcgcaaga agcaaaatca
    29881 gaagataaaa ttgtgatgga attgtctagc ttccaactca tgggaatcac agatttccgc
    29941 cctcatatag cggtgattac caatatctat gaagcacata ttgattatca tggtacaaga
    30001 aaagaatatg tcaaagctaa atggaacctt caaaaaaaca tgacagaaaa agattatctg
    30061 atccttaact ggaatcagtc agaactacaa gaattagcgc aaagaacaaa agcacgcgta
    30121 ctgcctttct cgacaaaaga agtgttggaa gacggtgtgt atgctgacga ttactcaata
    30181 tactataaaa aagaaaaaat aatggaaata tctgaacttg gcgtaccagg taagcataat
    30241 gtagaaaatg cactagctgc tatttctgta gcaaaattgt atggtatatc aaatgaagct
    30301 atacgagaaa cgttgcattt tttccatggc gtaccacatc gtacgcaata tgtaggagaa
    30361 atccaaggca gaaaatttta taatgattca aaagcaacca atattttagc gactaaaatg
    30421 gctctaagtg gatttgaaac gtctaaggtc gtgttattgg ctggcggatt agacagagga
    30481 aatacatttg atgaattaat tccttcatta aaaggaatca aagcaatggt cgtatttggc
    30541 caaacaaaag aaaagttgat ggatgctgga aaaaaagcag gcatcgagac aatagtaaca
    30601 gccgattctg tagaacaagc agttccttta gcattagaaa acagtacaga tggtgatgta
    30661 gttttattat caccagcgaa tgccagctgg gatcaatacc caaattttga aactagagga
    30721 aacagattca tggaagctgt gaaccgatta aaatagaaaa agtagaagcg agcgataaaa
    30781 aatgaaaatt ttggttacag gcggaggaac agggggacat atttatccag ctcttgcctt
    30841 tgtcaattat gtaaagactc aagaaccgaa tgctgaattc atgtatgtgg gagcaaaacg
    30901 aggtctagaa aacaaaatcg tccctgatac aggaatgcct ttccatacct tggagattca
    30961 aggatttaaa cgaaaaattt ccatgcataa catcaagacg gtccgattgt ttcttaagag
    31021 tatccgtcaa gcaaaaaaaa tcctcagtga ttttcaacca gatatcgtta tcggtactgg
    31081 aggatatgtt tccggagcgg tagtgtacgc tgcagccaaa atgggtattc caacagttat
    31141 ccatgaacaa aacagtgtac caggtgttac caataaattt ttaagccgat atgtggatcg
    31201 gatcgctctt tcatttaaag atgcggcttc cttttttcct gaaaacaaag cagttcttgt
    31261 aggtaatcct cgagcacaag aagtagcaga tactaaaaaa tcagatattt tgaaaacatt
    31321 tgatttggat ccagaaaaga aaacagtctt gatttttggc ggtagtcaag gagcacttaa
    31381 aatcaatcaa gcagtatcag tttttttaag tgaatatatt ccttcttctt accaagtttt
    31441 gtatgcttca ggagatagat attatgaaga gatcctaaat aaaataaatc ctgaaataca
    31501 aaatattagt gtccagcctt atatcaaaaa tatggcagag gtcatggctt ccagtgatct
    31561 gctggtgggg cgtgctggtg caacttcgat cgcagaattc acagctctgg ggttaccggc
    31621 agtgttggtt cccagcccat atgttacgaa tgatcatcag actaaaaatg caatgagtct
    31681 tgttcacgca ggagcagcaa aaatgattgc agataatgaa ctaacaggtg aatcactaag
    31741 tcagacagtc aacgaaatca tgggtgatga agaattgcaa aagcaaatgt gccgagcttc
    31801 taaagaacaa ggaatccctg atgcttcaaa acgtctttat gacttagtaa aacaaataat
    31861 caaaaaataa aggcggtggt cttagtgagt aagaaaaaag aaccaaaaaa attgtctcat
    31921 ccgtcttctg atgaaaacaa tttgactcct tggcaaaagg ctaaccgaaa atatctggca
    31981 gaacaagaga tgaaaaacca agagccaaaa aaagaagaca taacattaga tacacccctt
    32041 tcagattctg aagaagaaaa aacggttcaa gaggaacagc aagaagaaag ccagtccgag
    32101 aaaaaaatcg aattttttga agagatcaag caaaagaaaa acggtggacc atataacggt
    32161 tcttttttga accggctgcc caatttaaaa agtcaacggc acaaagtact atttcgtcgg
    32221 ttagctttga tcatcactgt attgggtata ccgctgattt ttttgatcta ttatgtttct
    32281 ccttatagca aattacaagc agttgcagta tcaggcaata agacagtcaa cagccaagaa
    32341 atcatctctg atacaaaatt gtcattagga gaaaatgttt ggggacagta ttttcatcga
    32401 tctacttata tcgatcgcct taaaaaagca cagccaagaa tcgaaacagc aaatatacat
    32461 ttcaaaggga tgaatgaatt cgatttggat gtaacagaat ataaagagat tgctttgatc
    32521 gcaaaaaaca atcaatacta cccagtgatt gaaaatggaa cagtattaga tgaaaaagta
    32581 gcaaatccta caaaaaatct ccctatcttg gaagagttca aagactctgc aaaaatcaaa
    32641 gaattaacaa agcaatacaa tcagttatca tcagaattac aaaaagcaat ttctgaaatc
    32701 aagtacacgc caagagcaag caataaaaat ctgatccaat tgaatatgaa tgacggcaat
    32761 caagttattg taaatatcaa taatcttgcg aaccaaatga aatattattc gcaagtagcc
    32821 aaagatatgg atgaaaaagg tgtgatcgac atggaagttg gtatcttctc gcatccttac
    32881 ggtagcactc aagaaacgga agaatcgggc caatcggaag aaacaactga aagtgaagaa
    32941 aatgcggatc aaacaaacga atcaagtgaa gaggcagaaa cgcaaaaagc acaaacacct
    33001 ggatcgtcta gcacaaacgg atcatctgat tcaaatgatt caagtgaaac aagtcaaata
    33061 gaagataatc caagtacaac tccgtcaagt ttaaatgaaa attgataaaa caactttaaa
    33121 aagtaagaat gtactttcta aaaaccagta atttatggta gaattggaag cagtgaatat
    33181 atctattaat aagtatattt taaaacggac gctagttgtt cgcttgtata tggaatagta
    33241 agtaggaggg aatcccattc atggcaaaaa caggaatgta tgtaggcctt gatattggaa
    33301 cgacatctgt taaagttgtc gtggctgaat atatcgacag ccaaatgaat attattggag
    33361 taggaaacgc aaaatcagaa gggattaacc gaggcattat cgttgatatc gataaaacgg
    33421 tccaagcgat acaacgagca gtacgacaag cagaagaaaa agcaggtatc cagatcaaag
    33481 gcgtaagtgt cggtttgcca gcaaacatgc ttgaagtaga aaattgccag ggaatgattg
    33541 cagttaacgg agattcaaag gaaatcaccg atgaagatgt cagaaatgtg gcttcagctg
    33601 cccttgttcg ttcaatacct cctgaacgtc aaatcgtgtc aatattgcca caagatttta
    33661 ctgtcgatgg gttcgaggga atcaaagatc ctcgtggaat gatcggcgta agattagaaa
    33721 tgtacggtct gttgtttaca ggacctaaaa cgatcatcca taacatacgt aaatgcgtgg
    33781 aaaatgccgg attaatcgtc aacgaaatgg tgattactcc tttagcgctg actgaatcaa
    33841 ttctttctga cggagaaaaa gattttggta cgatcgtcat tgatatgggt ggcggccaaa
    33901 caacaacggc tgttatgcat gataagcaat tgaagtttac caatcttgac caagaaggtg
    33961 gcgaatttgt cactaaagat atctctattg tattaaatac gtcatttaac aatgcagaag
    34021 cgttgaaaat aaactacggg gatgcatacc cagaacggac atcaccagat gaagaatttc
    34081 cagtagatgt gattggtcag tcagaaccag tgaaagtcga tgaacgctac ttgtcagaaa
    34141 ttatttcagc tcgaatggaa cagattttca acaaagcaaa agatgcattg gaccaaatcg
    34201 aagcattaga gttgccaggt ggcgtcgtat tgacaggcgg agctgctagt cttcctggtg
    34261 ttgtggactt ggcacaagaa atcttcggcg taaatgtaaa attgtatgtt ccaaatcaca
    34321 tggggctgag aaacccggta tttacaaatg taataagtat cgtggattat tcagcaaact
    34381 tgagtgaagt gtatcaattg gcaaaaattg ctgttacggg tgaaacctca gcagctcgtc
    34441 aaatggtagt tgaacaagaa acaacaaaca catatgagag ctatgaagca cctgaagaaa
    34501 caatctacga tgagccggaa ccaaaagagt ccggagaaaa tgtaaaaaat aaaatcaagg
    34561 gtttctttac aaatattttt gattaattca tcctgggagg aataagatat ggaattctca
    34621 attgataata atatcaatga cggcgcagta atcaaagtca tcggagtcgg tggtggcggt
    34681 ggaaatgctg taaaccgaat gattgaagaa aacgttaaag gtgttgaatt cattacagcc
    34741 aacacagacg tgcaagcatt aaaaaattca aaagctgaaa cagttatcca attaggcccc
    34801 aaatatactc gtggtttagg tgctggttct cagccagaag ttggacaaaa agcggcagaa
    34861 gaaagtgaac aatccttacg cgaagcttta gacggtgcag atatgatttt catcactgca
    34921 ggaatgggtg gaggaaccgg tacaggtgct gcaccaatcg ttgcaggtat cgcgaaagaa
    34981 ttgggcgcat tgactgttgg tgtcgtgaca cgtccattca cttttgaagg accgaaacgt
    35041 ggccgttttg ctgcagaagg tatcgctcgt ttgaaagaaa atgtggatac attattaatt
    35101 atctcaaata accgtttatt agaagttgta gataaaaaga caccgatgct tgaagctttc
    35161 cgtgaagcag ataatgtttt acgacaaggt gtacaaggta tctctgattt gatcacagcg
    35221 ccaggttacg ttaaccttga ctttgctgac gtgaaaacag tgatggaaaa ccaaggtaca
    35281 gcattgatgg ggatcggtgt ggctagtgga gaagatcgcg tagtagaagc aacgaaaaaa
    35341 gcaatctcct ctccattgct tgaaacatct attgatggag ctgaacaagt acttcttaac
    35401 atcacaggcg gtttggatat gacattgttc gaagcacaag atgcttcaga tatcgtagca
    35461 aatgcagcaa ctggcgatgt aaatatcatt ctaggtacat ctatcaatga agaaatgggc
    35521 gatgaaatcc gtgtgacggt catcgcaaca ggtatcgatg aatcaaaaaa ggaacgcaag
    35581 tctagccgac cagctagaca agcacaaatg caatcacctg ctcaaaagac tgtattagat
    35641 atggatcaag ctaaaccaac ttcttcagaa gaagaaaaca gttttggcga ttgggatatc
    35701 agacgtgaac aaaatgttcg tcctagagtt gatgattcca attttgacaa tatcgaaaaa
    35761 aaagaatttg atacattcaa ccgcgaagaa acaaaatctc atggcgatga tgaattaaat
    35821 acgccaccgt ttttccgtcg taaaaaataa aggagaggaa gagcacatga ttgctgacaa
    35881 cttgcagaaa atcaagcaag aaattcagga ttcgtgtgct cttgttcatc gtgatccgaa
    35941 agaagttaca ttagtagctg taacaaaatc tgttgaatcc gctcaagctg aagaattggt
    36001 tagagctggc gttatgaatt gtgcagaaaa ccgagtagat aaattattag aaaagaaaca
    36061 agatttattt gattttcctg aaataaagtg gcatttaatt ggtaatttac agcgcagaaa
    36121 ggtaaaattg attgtaaatg aaattgatta ctttcatgcg ttggacagtt taagattagc
    36181 agaagaaatc cagaagcggt tagagaaaga attatcctgt tttattgaag taaatgtttc
    36241 tggtgaggaa tcaaaacatg gaatttcacc agatgagctt ttaccgtttg tggaatcttt
    36301 agctgcctgc acaaaaatca aggttgttgg gttgatgact atggcgccta aagaagcttc
    36361 tgatgagcaa atccataaag tgtttggtac gctgaaagaa ttgcgtgatc ttgttcaagc
    36421 aaaaggttat gcccatgcac catgtagtga attaagtatg gggatgagtc aagactttca
    36481 aattgcgatt gaagaaggtg caacatttat ccgtgtagga acagcgctat tcaaagacga
    36541 ataaaggagg aacaaaaatg ccgcttttaa acagaggagc aattgctaat tttttcggat
    36601 tagctgatga ggaagagtac gaataccaag attatcctga aacaacaaaa aaggctgtac
    36661 agccaactaa aaaaaataca acacagaatc aatcaactca ttctgtatct gctgcgaata
    36721 ctcatgaaaa acaaaaaacg gcgacttcac gtcctgtaac gcatgaaagt attcagccgg
    36781 ctgaaaagaa aaaagtaatt gaactacatc aatcacctac aaaaactgta gcgaaagaaa
    36841 gaacagcttc aagaacctct acacaaccta aagcaaatct tgctggaaaa atcactgtaa
    36901 tggaaccaag agcttattct gaagctatgg cgattgctaa acgaatcatt gctggagaag
    36961 caaccttagt gaattttcat ttagtagaag aaaaacaagc gagaagaatc gttgattttc
    37021 taacaggaac agtttatgca ctagatggag atatcaaacg tgttggagat gagatttttc
    37081 tttgtacacc gactgggatg gagattgaca gttcaaccgc tcaatctttt gcagatgcga
    37141 atttgtttga tctgtaagag gaggaactac gatttatact attgcactat tacttcattt
    37201 acttcaacgg gcagcctatt tctatagcat tatcttgatg gtatatgcat tgctctcttg
    37261 gtttccaggc gggtatcaat cagggctagg acgattatta gctaagatct gcgaaccgta
    37321 tatcagtttg ttcgatcgtc tgcctttgag gattggaccc ttggatttca caattttagg
    37381 ggcaatcctg atcttgaact tagccgtaca aggtttagca cagatcgtta tatggataac
    37441 tgtccattta gtttattgat tgaagcaaca aaatctttat tctcagaata gataggaggg
    37501 gaaaattttg gatgcaaatg tataccagca ctttcgggca gatgaacgta catttatcga
    37561 ttcggtaggc gattggattg aacaggttca aggacaatat gcaccctatc tgaccgaatt
    37621 tcttgaccca agacaatcct atattttaga aacactgatt cgacaaagca gtgatttgcg
    37681 tttcatgttt tatggcggat atgaacaggc agaaagaaaa agatgtttga ttttccccga
    37741 ttattacgaa cctgtagagg aagattttga aacagcattg tatgaaatcc attatcctag
    37801 caaatttgcc agcctaagtc atgggaaaat ccttggaact ttagtgggaa cggggatcaa
    37861 acgctcttac tttggcgata tcatctcgga cggtgatcgc tggcaagtat ttattgcaaa
    37921 tgaggtggat cattttgttg ctgcacaagt aacaaagatt ggaaaggttg ctgtacgctt
    37981 ggaaccaatc aaatacacag aaatgattat gccgaaagat agctggacac aagaaagagg
    38041 aacagttagt tccatgcgac tagacagtgt gatctctgag atttataata tttccagaca
    38101 gcggtccaag cagctgatcg aagctggtaa agtaaaagtg aactggacag aaaatacaag
    38161 acctgatttt gtacttgaac tattagatat tatttcagtc agaggttatg gcagagtcca
    38221 gatccaagaa cttgagggga agacgaaaaa agaaaagtac agggtactgt tcggtgtatt
    38281 acgtaaataa aataaaaagg tggtttactc atggctttaa ctccattaga tatccaaaat
    38341 aagacattcc cgacaaaaat gcggggatat aatcaagacg aagtagatga tttcttagat
    38401 ttagttgttc gtgactatga ggaattgact caaagaaatc gagaattaga aaaagcagtc
    38461 aaacattctg aagaaaaact tgagtacttc aatgaattaa aagatgcttt gaaccaatca
    38521 atcatcgtag ctcaagatac ggctgataaa gtaaaaacaa gtgcaagcaa ggaatccgaa
    38581 gtgatcgtta cttctgcaca aaacaaagca gatgagttag ttgcgaacgc tgaaaaacgt
    38641 gcacatcaat taacaactga tgcagaagaa aaagcaagaa aaatcttgac agatgcaaca
    38701 gaaaaagcaa gacagcttgc gacagaaact gaagatctta agaaaaaaac acgagtattc
    38761 catcaacgta tcagcttgat gcttgagtct caattagaac aagtgaaaag tccggaatgg
    38821 gatgaaattt tacaaccatt ctccagctac gtgactgaca gccatgaagt gatcaaagaa
    38881 gttttagcaa aacagcttga caatgaaaat gatactgatg taaactcaga cacaggagtc
    38941 atagaagcac ctatgacggc atttgataca caggctattg atatctcgat cgtgcctggt
    39001 gaaaatgaaa acgagtagaa cagaaaagca ctttgttatt cttaaagcga gccgatgaga
    39061 gtggaagttc ggtaagacac tgcatggtga aagatcctct acttgagacc tgcttttccg
    39121 aaattagagt aagaagcagc ggctgacctc gttattggtc acagagagtt tgcactagat
    39181 agatgcaact aaatttgggt ggtaccacgg catgttcgtc ccttgcggct gaacatgctt
    39241 ttttgtgtcc ggaaaacaaa taaaggagtg tatggttgaa tgaaaatgaa agaaaccttg
    39301 caattaggaa aaacaaaatt ccctatgcgt ggtaatttgc caaaccgaga aggcgagtgg
    39361 caaaaagaat gggaagaaaa tcgaatctac caaaaaagac aagagttgaa cgaaggaaaa
    39421 cctagctttg ttcttcatga tggacctcca tatgccaatg gtaatatcca tttaggacat
    39481 tcattgaata aaatcagtaa agacattatc attcgttcaa aatcaatgtc tggctatcgt
    39541 gcaccatatg ttccaggatg ggatacacat ggattgccga ttgaacaagt attggctaat
    39601 aaaggcgtga aacgtaaaga aatgtccatg gcagaatact tggaaaaatg tcgtgaatat
    39661 gcactttccc aagtagataa gcaacgagca gatttcaaac gcctaggagt tgcaggagat
    39721 tgggaacatc cttatgttac attagatcct tcttatgaag ctgcacaagt acgcgtattt
    39781 ggtaaaatgg ctgaaaaagg ctatatttac aaaggattga aaccaattta ctggtctccg
    39841 tcaagtgagt cttctttagc ggaagctgaa attgaatata aagatgtaaa atctccatct
    39901 atttttgtag ctttcaatgt caaagacggc aaagatattc ttgatgaaga tacagctttt
    39961 gtcatttgga cgacaacacc atggacattg ccagcaaacc aaggtattgc tgtaaatcca
    40021 aaatttacgt atgtattggt ggaagccgat ggccgtaagt ttgtcgtagc gaaagatttg
    40081 ctagaaacag tccaatcaga aatcggttgg gaaaatgtaa cagttctaaa agaatttgct
    40141 ggacaagaaa tggaatatat gactgcaagt catccattct atgaacgtga atcacttgtg
    40201 attttaggcg atcatgttac tttagacgct ggtactggat tagtccatac tgctccagga
    40261 catggggaag atgactatat cgctggtagc aaatataaac tatctgtagt gtcacctgta
    40321 gactcaaaag gattgtttac agaagaagca cctggatttg aaggtatctt ttatgacaaa
    40381 gcaaatccta tgatcactga tctattaaaa gaaaaaggag ctttgctgaa gctagatttc
    40441 tttactcata gttatcctca cgattggcgg acgaaaaagc ctgtgatcta tcgggcaact
    40501 ccacaatggt ttgcatctat cgataaattc cgtcagaata ttttggatga agtagaaaaa
    40561 gtagagtggg ttatcccatg gggtaaaaca cgtttgtata atatgatccg tgaccgcggc
    40621 gattgggtaa tttcccgtca gcgtgcatgg ggagtaccac ttccgatttt ctatgctgaa
    40681 aatggagaag caatcatcac accagaaaca atcgaacatg tggcaaaatt atttgctgaa
    40741 catggatcaa ttatctggtt tgaaaaagaa gcgaaagaac ttcttcctga aggatttacc
    40801 cacccaggtt ctcctaatgg cgaatttaca aaagaaaaag atatcatgga tgtctggttt
    40861 gattctggtt cttctcatga agctgtttta cgtcagcgac cagaattatc attcccagca
    40921 gatatgtatt tagaaggatc agaccaatat cgtggctggt tcaactcaag tatcacgact
    40981 agtgtggcta tcaatggtgt agcaccatac aaatcagtta tttctcaagg atttgcttta
    41041 gatggtgaag gccgtaagat gagtaaatct ttaggaaaca tcattgcacc agaaaaagtg
    41101 atcaaacaat ttggtgcaga tatccttcgc ttatgggtat ctagtgtaga ttatgaagca
    41161 gatgttcgtg tttctatgga tatcttaagt caagtggcag aagtatatcg taaaatcaga
    41221 aatacgatgc gtttcttatt agcgaataca gaagatttcg atcctaaaaa agatactgtt
    41281 tcgtacaatg atttacgttc tgtagacaaa tatatgaccg ttcgattgaa ccaagtaatc
    41341 aaagaaatcc gagaagaagg gtacgacaaa tataacttca tgcatattta ccgtacagtt
    41401 atgaacttct tgacggttga tctatcgtca ttctacctgg attttgctaa agatgtagtt
    41461 tatattgaag cagaagatga ttatcaacgt cgttgcatgc aaactgtttt ctatcaaaca
    41521 gctgtagctt tgacaaaatt attgactcct atcattccgc atacagcaga agaaatctgg
    41581 tcattcctga aagaagaaga ggagtacgtt cagttaagcg agtttcctgg ttatgaagag
    41641 tttgcaaacc aagaagaatt gatggatacc tggactgctt tcatggactt ccgtgataat
    41701 gtcttaaaag cattggaaga agcaagaaac agcaaattga ttggtaaatc tttggaagca
    41761 aaactaactg tttatccaaa acaacaagtt cgtgaaatgt taaaagcttt ggatgcagat
    41821 attgcacaat tattgatcgt ttcaccagac tactttgaag ttatggcagc agatgaacaa
    41881 gtgccagata acgctatggt atttgatgat gttgctatta cagtggaaaa agcagacggg
    41941 gaaacttgcg atcgctgtcg ccaagttcgt aaagatgtag gagtagatga aaaacttcct
    42001 catctatgcg gtcgttgtgc aaaaatcgtt gaagacttct atcctgaagc tgtagctgaa
    42061 ggattcgaag aaaagtaaaa gggaaaaggg ggtgtgacat gttctttgtc acaccccctt
    42121 ttgttatcaa tcgtttaatt ttccacgttc acgataccag ttatctggtt gccagatcca
    42181 ttcgaatccg tctttttcaa gtagttcgaa agctgcttta ggtcccattg atccagcttt
    42241 gtagttggga aaatccggtt gtgtttggtc ccaagctttt cgaatgacat ccacgatatg
    42301 ccaagattga gcgacttctt cccaatgaga gaagttcgtt ccatcaccat tcaacgcatc
    42361 taatagtaat ttttcgtaag cttctgggct attttcaacc atttctgcac tatttctaaa
    42421 ttccaatttg attggttccg tttcaaatcc ttgccctact tctttcccat tcaaggataa
    42481 agaaaatcct tcagttggct gaatatagat cgttagaaca ttaggtggaa gtgtcgtatc
    42541 gtcacatggt tcatcgacag atgttttgaa tacattaaca ggaacttgtt tgaatacgat
    42601 gttgatccga gtcccttttt cagtcaaacg tttgcctgta cgtacataga aaggaacacc
    42661 tgaccaacgg aagttatcga tagtgaattt gcctgcgacg aatgtttcag tctctgattg
    42721 agcatccact ttgtcttctt cgcgatagcc cttgaattgt acaccatcca attcaccttg
    42781 agcatattgc ccgcgaacga agttttctaa cacttcttct tcgttataaa tgcggatagc
    42841 tttcaatgct ttgatctttt ctgtacggat ttctttttcg gaaaatgcta ctggcggttc
    42901 cattgcaagt aatgctacaa cctgtaaaat atgattttgc accatgtctt taagtgcgcc
    42961 actatgatcg tagtagcccc cgcgatcttc tacaccgagg ttttcgccaa aagtaatttg
    43021 gatattgtcg atatagcgat tgttccattg agcctcaaaa atattattgg caaaacggat
    43081 agctgagatg ttttggatca tttcttttcc taaataatga tcaatcctga aaatatcttg
    43141 ttctgggaat acttctctga tttgctggtt caattcaaaa gcagactggt aatcagaacc
    43201 gaacggtttt tctatgatca aacgattgaa gccatttttt gtcataatat tttgtgattt
    43261 caaatgagaa acgatcgttc caaaaaattg aggtgccata gccaaataat agacacggtt
    43321 accgtttaat tgatagtgtc catctaatcg gtcagacaat tcttttaatg tctgataatg
    43381 cgttgaatca tttacatcat gggattgata gtaaaaatgg cttgcaaagg tagttgcttc
    43441 ttcatctgaa ggattcaatg cacggattgt ttctttgaca acttcacggt agtgctcatc
    43501 gctccaggga cggcgagcgg ttccgattac agcaaaatgc tcgcctaaat ttccttttct
    43561 gtaaaggcgg aacaaagacg ggtacaattt acgctgggct aggtcacctg taccgccgaa
    43621 tatcgtaaac aatacatttt tttcattaga catgtggata cactccttta tattttatcg
    43681 acaaaaatcg tgctggccgc cttaaagctg acggggattt tctttctatc attttggata
    43741 gtgatcggtc cttcataagc tgcgatgtca acgatctcat attcttcgtt gatattcagg
    43801 tcaatagtga ctaagtaatc caacaattct ttttcatcaa gcactcgtgc gatacgcact
    43861 tttgtaccga caggatagga ctccaatgtt tgtcggcgtt cttcatggac aacttcctct
    43921 tttttaggaa tcgatcctcc atgaggacaa tgttcaggat gattcaaata gttttctaaa
    43981 tgatctgcaa gatgttccga agtcacatgt tccaatactt cagcatcatc atggacttca
    44041 ttccagctat agtctaaatg ttcaactaaa aaaacttccc ataagcgatg tttgcgaatc
    44101 agactgctag ctttttgcag tccgcttgca gtcaattgta caccttgata aggggaatgt
    44161 tctacaagct cttctttcac aagtttagaa atcatttcac tgacagatgc agctgagaca
    44221 tctagactag atacgatttg tttattgttg actttcgttg tatctccacc tagttctaaa
    44281 atgattttca aataatcttc tcgatttggt gtcatattcg atcatccttt catggtaaaa
    44341 aaccatcatt agtaagtgta acaaaaaaaa cgttaattga ctatttttct aaataaaaaa
    44401 caaaggattt ttagacgttt ttacaacaaa aaacgaaaga tgatagagaa gactcattct
    44461 ttcgtttata aaggatatat atatacattg ggagggatct atttttcaga taacaactta
    44521 tatgaagtgt ctgcagattc gtcataaaga gtttgttcgg gataagaaaa gataagataa
    44581 aaaccatact gatcacgtac gactaaacga tattcatcta tttctaccac agatgtgcca
    44641 agagactgac cgttgatcaa aaacgtgtaa tcagatgtaa tctgtaattg ttgtttttgc
    44701 tggctatcac taaagaacca tgtaccttga tactttgaga gtccttctga cttttccgct
    44761 tgttttttct ttttcaagta aataaaactt gagagaacgg taatgagtga accaagaaaa
    44821 tagatcattt tttttatcac caaatcactc ctttatgaga aaataatcgc atagacatat
    44881 tctttcttct ttatttcatt gtaccgaata aaagctaaaa tttctctaag actttcgact
    44941 ggttttttta ctgtttttct acaaattatg ataggataca tagtaggaag aaaggtgaca
    45001 taatttgaag tatcagcaag tggaaatagc tttttttatt gaccgcccga atcggtttat
    45061 agcacattgt atgaaaaaag acggagaaat aattattgcg catgtcaaaa atacaggtag
    45121 aggaaaagag gtcctgatac caggcgctga agtggctatt gtatttgctc ctggacctaa
    45181 acggaaaacc gcctatgatc tgattgctgt aaaaaagaag ggtgactggt ttaatatcga
    45241 tagtcaattg cctaatcggc tggcaattga tggtatttta gacggcacga tccttttacc
    45301 aggattaact ggtccattta cggtatacaa gcgtgaagta acttatcttc actccaaatt
    45361 tgatatttat ttagagacgg tggtcggaca aaaagcattt gttgaagtca aaggtatgac
    45421 acttgaaaat aaagcgattg gtgcttttcc tgatgcgccc acactcagag gattgaaaca
    45481 tataggagaa ttaacgtatg ccgcacaaga tggttatgcc gcttatgtct tgtttattgc
    45541 tcagttcgaa catcttcatc tggcaacgat ccatgaagaa atgcaaccag cactagctga
    45601 tatggttcga catgcgcagc aaagcggagt tcaaatcctc gcctataatt gtcaagtatc
    45661 ggcagatgag gtaactgtca agcaggccat tccatttgat gtagatatcc cttttgaaga
    45721 tcctatacaa tagaaagaag gtaaaataat gatcaactta ggtattatcg gaacaaattg
    45781 gattactcat caatttgtcc aagcagcttt aagcacaaaa aaatatgaat tgacagcggt
    45841 atattctaga catttggaaa aagcacagga atttggttct cattatgaag gggatatcga
    45901 attcgcaaca gatttgaaga cttttttcaa tattactcat atggacacca tttatattgc
    45961 ttctccgaat agtcttcact ttagtcaagc taagcaagca attttggcag gaaaaaatgt
    46021 gatagttgaa aagccagctt tttcgacgcc aaaagaaatg gatgaaatca tccatttggc
    46081 aaatgaacac aatgtgtatt tttttgaagc agcaagaaat atccatgaat caggttttaa
    46141 aaagattgcc gattacttac cattaaaaga cgagataatc ggtgcaaatt tcagctatat
    46201 gaaatattct tcgcgctatg accaagtatt agatggagaa gaaccgaata tcttctcgac
    46261 tcatttctca ggtggtgcat tgatggattt aggtgtttac cttgtatatg cagctgttgc
    46321 ttggtttgga atgccaaaag aagtccatta tttcccaaga aagatttcta ctggtgtaga
    46381 tggtcttgga tgggggatct tacgttatga gaattttgat gtatcgatcc agccaggtaa
    46441 aatcggtgat tcttatttac cgtcagagat ttattttgac tcaggaacgc tagtgatgaa
    46501 tggagtcaat gcaattgaac aagcacaatt tcatgatcgc gcgcataaag aaatcattga
    46561 tttggcggta acagctgcag aaaatccaat ggcagatgaa gcagaggatt ttgccaatgt
    46621 catggctcat ccgaaagatc cagcttgggg attgttgtat gaagagtgga tcgagctagc
    46681 tagaaatgtg aaccaagtga tttatgattt aagaaaaaat ggcggcatca aattcgatgc
    46741 agataatgag gaagattttt aatgaacgaa tttgtacaag tatttcccga aaattggcag
    46801 aaaaaatatg aggataaagg gtttatctca ccttctctta ttcaacgagc agtttttcag
    46861 ccactaagta ataaacaaag tatagtagcg gtttctccta caggttcagg taaaacactg
    46921 gcttatcttt ggcctctact actaaacgtg gaaccaggtg aagcaagtgc gttggtcatt
    46981 tttgcttctt ctcaagaact agctattcaa gtagcagatg ttgcacgtga atggggaaaa
    47041 gacaaagagt tgaaagtcca atctttggtg ggcggagcca acgtcaaacg tcagattgag
    47101 ggtttgaagg aaaaaccaga aatcttgatt ggtacaccag gaagaatatt ggaattgatg
    47161 aaagcaaaaa aaataaaagc acatcaagtg aaaacaatgg tatttgatga agcagaccag
    47221 ctatttgacg ctgggaacag ccaaatcatt gatcaaattc ttcatcaagc tccaacagag
    47281 tatcagctag cttttttttc agcgacagca gatcgttccc ttgaaagcat tgaaaaaatc
    47341 actgggaaaa cgtttgagac aattgacgtg acagcagagg acgattctcg aaaaggactg
    47401 agacattact ttttacgtgt accgatacga aagaaagatg actacttgag acgtctcacg
    47461 catatagaag attttcatgg tctgattttc tttaatcaac tgactgaatt aggcgtaatg
    47521 gaagaaaaat tgctgtatag aggtgtaccg gtaggaagtc tggcctctga tcagaacaag
    47581 ctactccgaa aagcagcgct agaacaattc aaaaagaaaa tggtcagagc acttttgacc
    47641 acagatgtgg cggctagagg tttagatatt acaggtcttc cttatgtagt gaatgcagaa
    47701 gttccgttaa gtgaagaagc ttatttgcat cgttcgggaa gaactggtcg aatgggtaat
    47761 gaaggaatag taattacact cgtacaagat aataatctaa gagatttaaa acggctttta
    47821 cgtccatcaa atataacact tgaagagatt tttttatacg gaggtaagct gcaaactgag
    47881 cagccaacca aagaagatgc agctcctgca gtaaataaaa agtatgcgaa aactttttct
    47941 ggaaaggagc agaacccaga atataacaaa gaaaaaataa gaaaaaaagt taaaaataaa
    48001 tcaaaaaaag agagaaataa aggagcaagg cgcaaataag cgccttttta tgctatgatt
    48061 atgaaaacgg gaacaaaatt ggttgccaga ggaggggtta tgtgaaagaa cgtcaagaag
    48121 agatcttgca gctattgaca gaaaaccaac aaggattgac agcaagtgat gtagctgagc
    48181 gcttaactat cgatcgaagc aatgcaagcc gatatttaag tgaactatac aaggcccatc
    48241 atattgtaaa aaccgctggc cgaccagtcg tttactcttt gccaacggaa aagtcaaaat
    48301 ctgatgaagt gcatgtcgac tcttctacgc aagtgacttt tgaaacactc gttggagaaa
    48361 atgattcttt aaaagtcagc atacagcagg caaaagctgc gattctttat ccaccgcggg
    48421 ggttgcatac gattattttt ggagaaactg gtacagggaa gtcgatgttt gctgaatgta
    48481 tgtatcattt tgcaatcgat tcagagatgc tttctgcaga tgcgccattt gtttctttca
    48541 attgtgcgga ttatgctcaa aatccgcaac tgctctttgg acacatcttt gggatcaaaa
    48601 aaggggctta cacaggagca gctcaagata gtcccggtct aatagccaaa gcagacggcg
    48661 gtatattgtt tttagatgag atccaccgtt taccgccgga agggcaggaa atgctgttta
    48721 ctttcattga taaaggtatt taccgtccgc ttggagaaag tgcacaagta catgaagcat
    48781 ctgttcagat cattggggcg acgactgaat cttcagaaag tttcttatct acgttcaatc
    48841 gacggattcc tatggcaatc accttgccga atcttgcttc acgttcactg gatgagcgtt
    48901 atgaaatcat ctctcttttt atcaaacaag aagcgaatcg tttgaatcaa cgtatcgata
    48961 tcgaaaaaga agcgattctt gccttcatgc tttatgatgc ggaaggaaat atcggtcaag
    49021 tgaaacgaga cttgaaacta gtctgtgcaa aatctttttt acattaccgt actcatcatg
    49081 agaaaaagct aatcattcga aaagaagaac tttcccttca agtacaaaaa ggattactga
    49141 aaatcaagga aataccggaa cgtctcgacc gattcatgga cagcaaaagt cagtatttga
    49201 catttgaacc aggatttgct gatgttgtct ggtcacaaga tccagaacga aacatgcagg
    49261 tctataacga tattgaggaa aaagtcactt ctctttccga aaccggtgtg gagaacattg
    49321 atctagagac cctcatttca aaagatgtgg atgcttattt ccagacatat gtcaaagaat
    49381 tgactaaaag ttcgattccc aaagatcttt tgccagatga aatctggcgg ttaaccaatc
    49441 aattgtatga tgtggctgaa aaacggttag accgtattta taatgaaaaa gctcgttttg
    49501 cttttgcttt gcatctgcaa agtacattgg atcgtgtgag agaaggtcat atgatcgtcc
    49561 acccagattt gaataacgta cggaaaaatt ttaagaggga attccaggta gcaatcgatc
    49621 tttcaacgat cattgaagaa gaagaacaag ttgagattcc ttttgatgaa atcggcttta
    49681 ttagtatgtt tctttctatc aatctaggag aaagtgagcc tgttaaagaa aacaaagtag
    49741 atgttctcat attgatgcac ggcagacaga ctgcttcaag catgttggag atggcacaag
    49801 aactattagg aacaaaagta gggaaagctt ttaatatgcc actggttatg gaagttcaaa
    49861 caatgtatga agaagtgtta caatatgtca aaaactatca agagcatttg acgaatggcc
    49921 tactcttgtt aacagatatg ggatcattaa acaccttcag tgatttgatt tatgaagaga
    49981 ctggcattcg gacgaaagct atcacgatga caagtaccat gattgtgata gaagcgatac
    50041 ggatggcaag tgttggaaga agcttagaag atatttatca aaatatccag ctttcctttg
    50101 aatcgatcgt acgtgatcag ttcaaagcgg aaaagtctga aactttacag cgtaaaaaag
    50161 ccgtaatcgt gacatgcttt actggtgaag gagtagctgc caaattatat cagcgtatta
    50221 tgccagtgat taatcaaaat aaggtagaac tgatccagat gcagttttta gaacgtgaat
    50281 cattcaaaaa acacatcgat gccttattgg aagaatatga gatcaaagca attgcgggaa
    50341 cagttgatgt tgattatcaa aatatcccat ttttttcagc ctatgacgtt tttgatgatg
    50401 aaaaactaaa tattttaaag cgtatagctg atgatgagat cccgacagct aaaattgtcg
    50461 attcacttgc tggatcatta agcgatgtct tttctattga tgaactgatc cgttcattgc
    50521 aaaagacagt tcagcagatc cagaccgatt tgtcgattat cgtggaacct ggagtagata
    50581 ctggaatcgt tatccattta gccttcatgt tagacggatt gatcaaacaa gaaagccatc
    50641 ggcaatttga gaaactgtct attttctcaa aaatttatcg tttggaaatg gatatcgttc
    50701 gggcaaactt gatggcacta gaacgaaaat atcaggtaaa aattccagaa gatgatatcg
    50761 catttcttac acaaatgttt cttgaaaata aaataaaaaa atgaacacac taatcatact
    50821 gtttacacac taaacatctg tgtgcgcact ataaaaaaga gctaaaaagc atgaaaacgc
    50881 tatttcagct ctttttttta ttggcatgct tcttgcgtta tataaaagtg taaaaaaata
    50941 aaaggagtgt taaaaatggc aaaaaaaaca atcatgttag tttgttcagc agggatgagt
    51001 acgagtttat tggtcacaaa aatgcaaaaa gcagcagaag aaaaagggat ggaagccgat
    51061 atttttgcgg tctctgcctc agatgcagat aataatttag aatctaagaa tgtcgatgta
    51121 ttattattag gtcctcaagt acgtttcatg aaagctcaat ttgaacaaaa acttgcgcct
    51181 aaaggtattc cattagatgt catcaatatg caagactatg gcatgatgaa cggagaaaaa
    51241 gttcttgcac aagcagaaaa tctaatgaaa taaatcaagt atgcagttgg gggtataaca
    51301 acgtggaaga tcaacaaaat ctagaagcca tcatggggtt aatcatgttt ggcggtaatg
    51361 caaagagcga tgcaatggaa gcgattgcgg ctgctaaaat cggtgatttt gagttagcgg
    51421 ataggaaaat cgctgatgca gaagaatcac ttatacaagc gcatcatgca caaacagaca
    51481 tgttgacaca agaagcacaa ggaaatcata tgcaagtgac attattgact gttcacagtc
    51541 aagatcactt aatgacatct attgcgttta ctgatttagc aaaagaaatc atcgaacttt
    51601 atcgtcgcgt atacagcgaa taaatacaac ttcttttttg aacggaagaa gaaaaatttt
    51661 tgaacggatt ttttattttg ttataaggag aactctcttt atataaaaaa cttataggag
    51721 gaagtaaaaa atgaacggac tatcaagttg gatggagaaa tacatcctgc cggttgccgc
    51781 aaaaatcgga gcgcaaaaac atttagttgc tttgcgtgat gcttttatcg gcacactgcc
    51841 cgcaacaatg gcgggatcag tcgctgtaat gatcaatgca attattcgtg atttaccgca
    51901 acagtttaac agtacctatg ctgacagttt agcagcagac aaaggaatct ttgccgtatt
    51961 gaatgtcatc atcggaatca atggttttgt ctggaacggt acactagcaa tcgccggatt
    52021 gatctttgcc ttttcttggg ggtataatct tgcccgagct tatggagtaa atgatctagc
    52081 tggggggatc gttggtttag ctaccttgat tcaaggtatt tcctttggtc cagcattatc
    52141 agcagcatta gatgtgaatg ttccttcaaa tgtcgcagat gcaatcaata gttctgagat
    52201 tggtgcaacg attgcagatg gcacactgtc agttggatta tggggttggc taaaactaga
    52261 tcatttgaat ggaaacgctt attttacagt tatgatcatg ggagctattt cagtcattat
    52321 tttttgtaaa ttgatgcttg ccaacattac gatcaaaatg cctgattcag taccaccagc
    52381 agtatctaaa gcatttgcag ccattttacc agcgacgatc gctttatacg tcattgctat
    52441 tatcaattat atcgtaggtc gtgtagctga tgggcaactg atcattgatt tggtgcaaaa
    52501 atatattgca gagccattcc taggattatc tcaagggttg ggagcagtat tgatcgtcac
    52561 agtatttgtt caagtattct ggttctttgg tattcatgga ccaaatgtat tggcaccagt
    52621 gctagaggga atctggggac aagcacaact aatcaatatt gatattttcc aaaaaggtta
    52681 taacggaaaa actggtacac cagccgtgtt agatgcaatc gaagatggaa aagcttatat
    52741 gtgggtccgc ggatcgttcg atgcttttgc atggtttggc ggatcaggcg gaacgatcgt
    52801 cttactgata gctatcttga ttttctctaa acgtgcagat tatctaacag tagctaaatt
    52861 atccattggg cctggtatct tcaatatcaa cgaaccagtg atgtttggtc tgccaatcgt
    52921 attgaatgca atcatgttta tcccattctt gattgctcca gtagtagcaa cagcaattgg
    52981 atggacagct acttatcttg gcctagtcgc tccggtatcg cagcaagtca catgggtagt
    53041 accgccgttc ttattgtcat tcttagcaac aggagcggat tggcgggcac cgatcgtaac
    53101 attagtatgt atggtcgtga ctttcttgat ttgggcacca tttgtcatct ctgcaaacaa
    53161 aatggaccct gccgcaggcg aacaacaata agatgaacgt gaggagcggg acgctttaag
    53221 tttcgttcct cataatgaat tttcgaggag gaactgaaat gattacaatt gccagtgcaa
    53281 tgcaacaaga tgaaatcaaa gaattgttag aaggatacaa tgaagaagga atgactttta
    53341 catttaaaga aaaacaagga atcaagctgt tctttgaaac aagtgcagaa gatccggaag
    53401 ttgcggctaa aaaagcaaag gaactaatca aagcacagcc ttggggaaca gtcctttatt
    53461 ttcaatcaac agctgtttag aaaatggttt aaaaagatag gatggttgag atgataaaaa
    53521 acggtttatt tttatgtagt attggttctt tgatcgtgtt gtatagtttg aatccacatt
    53581 cgcaaaggta tgatttgtat agtatggtca caggtctttt tctaacagga ttaggaatct
    53641 atttcttcat gaaggggaaa aaaagagaag aagcaaagga gaaaaataac taatggaaat
    53701 cacgaaaaaa ttaaatattc cagcatcgtt tttttacgat aaagttatgg actctgtcct
    53761 tttcgatgta cgtaaagcga ccggcaaatc agtaactaga aaacagctga aaaatttgga
    53821 gtacgtcaaa caattctcgc aaaataaccg ggctcgcata aaaatcgaag aagtcattga
    53881 aaaccagtct tataagtttc gtacatcgac aacgaagaat gaattcgtcg ttcattatca
    53941 aatcaaacca ttggacgatt cgacctgtga ggttcattac acagaaaaaa tggagtctta
    54001 tggcatgctt caaaagttga atgatatgct tttaggaaca atcttgatgt attttaaacg
    54061 gagacgtttc aaaaaaatgc tagatatgat cgaagcaacg tattaaatca atgaaacatg
    54121 gtactattta gttattgaaa aatgaagtaa gctagaagga atttttcata ggaagataga
    54181 tctttcctgc gaatcctcac tagctttttt gtaggaaggg gcagcaaaat gacaaacaaa
    54241 gtaagagagc taggtgtatc ttcgattgag gaaatgtttg atttagccac ttatgcattt
    54301 aatggggcag atacacctga aaggcgagaa cgttttcgta cacttgcgga gaattcttgg
    54361 aattacggat tttttgatga ggaggatcgg ctaacaagtc aagtcatggc gacccctttt
    54421 ccagtgaatt tttacggaca agagtaccta atggccggca ttggctatgt tgcttcttat
    54481 ccagaagcaa ggggacaagg cgggatcaat cagatcatgg aaaagatcct tgaagattgc
    54541 cgtgatcgta aagtaagctt atcttatctg gcgccattct cctatccctt ctatcggcgc
    54601 tatggctatg aacaatcatt tgataaaatc agttatcaac taaacagtcg tgatgtgccg
    54661 tttatcaaaa aaaaatctgg aaaaataaag cgtatggatt ttgaagatgc aaaagctgca
    54721 atcagaagaa tttatgaaca gatgccggaa aaccaacgag ctggtctgaa gcgcgaagac
    54781 tggtggtata cgtataaatt caagcaaaaa ggcaattacc agtttgcttt gcattttgat
    54841 gataatgagg aagcaacggg atatatcgtt tatcagcttg caacgtcttc ctttattatt
    54901 gcggagtggg gatttttgga tcatgatgct ttttgcagcc ttgttcgctt cgtaagttct
    54961 cacaatggtg cttttgaaac atttgaatat tcatgcggct acggcggtaa tgatcaaaat
    55021 tatctattag aaaatccatt tgcttctgta atgatcacac cttatatgat ggcgaggatc
    55081 gtagatattt ctttgttttt ggaaaattat ccatttaaaa agagacaagc tgcatttgcc
    55141 ctacaaatag aagaagatca ttatgcgaaa tggaatgaag gaatcgtgga agtaacaatc
    55201 gaaaatggaa aaaatcatct ctcaaaagta gaacaaacaa aactcccaat cgttcgagga
    55261 agtatccaac atatcacgca gctattatta gggtatcgta aagtggaaga gttggtattc
    55321 caagaaagaa tacaggtgac aaaagaatat gaagaaacgt taagctcaat cttgccacag
    55381 caacagccaa ttcttaatga ctatttttga taaaaaacgg gtgatgacaa tatttttgtc
    55441 accacccttt tgtttttcat ttggcatttg aaggaaaatg ttaaagtaaa tcctcttttt
    55501 tcgtaaattt gaattttttg ttttcttttg ttttagggtt ttcttttttg tggtcttgga
    55561 ttgcttcttt cgtaatcttt tcccgatttt ttttagatga atctaagcgt tctgcgtata
    55621 ctttttcttt tggcttatca ttcattggaa aggacctccc tacattattt cattctacct
    55681 ctagtttatc atctgttaag gactagtgga aacgttttgc ctaacttata agctgatttt
    55741 tttagcagat aagcttgcaa gtaaaaaaca aacaagctac aattgattta ttgaaattgt
    55801 tcctgtagct cagtaggata gagcaatcgc ctcctaagcg ataggtcgct ggttcaattc
    55861 cagtcaggaa cgtacaaata acccctgtat cctttgcggt acagggggtt attttgtata
    55921 agtagacatt gtagtttcta aactgctatg tcctaaacgt ttcgatacgc taagtatatt
    55981 tactccttga taaagtaaaa tagatgcatg cgtatgtctt agtccatcaa taaacagtcg
    56041 aagagaaaat agggaaaaat tgattactaa agaaaaactt actaggtcga cggtgtagtt
    56101 gattttcata cttcaataga ttcgaattaa acgatttcga ttggcttata ccagtcctat
    56161 ccaacgaaag atatatcgtt cttaactcta gcggtataac ttgaagcttt acgtaaagcc
    56221 aatgggaatg aactggagat gactttttat tatttttagt aatagctgat gggttaatac
    56281 ttacaacttt ccttttcaaa gtaccatata aaaaaacggt ggggttgttt agcgaaataa
    56341 aggatctaag ccaatagctt agatccttta ttctatgctt tatattattt tttgcatgtg
    56401 tgcgaaagta tactcaagcc caatcgccat ttcggaaaat cggtactgta gagccatctt
    56461 cacggattcc gtcgatatcc atcttgtcag atcctaccat gaaatctacg tgggtttgac
    56521 ttctattcaa tccagcttct gctaattctt catcggacat ttctgtaccg cctttcacac
    56581 tgaaggcata agcagaacct agtgctaagt gattcgatgc gttttcatca aataatgtat
    56641 tgaaaaagat gatgccagat tgagaaatag gtgaagggtc aggtacaagt gcgacttctc
    56701 ctaaacgacg cgcaccttca tctgtatcaa gcagttttgc taagacatct tctccttgtt
    56761 cggcagaaaa atcaacaact tttccatctt taaaagtaaa tttcataccc gagatgatgg
    56821 tgccagcata actcagtggc tttgtacttg aaatgtaacc atctactcga tgactgtcag
    56881 gtgctgtgaa cacttcttcc gtcggcatat ttgccatgaa tttttctcca cgagcattat
    56941 agcttccagc gccttcccaa agatggtttt taggcaatcc gataatgata tctgtaccgg
    57001 gagcagtata gtgaagggca gaaaattgtt ctcgattcaa ttcttctgct ttttttgcta
    57061 atttttcatc atgcttcttc catgctaaga cagggtcttc ttcatagaca cgagtcgttt
    57121 tgaaaatctg atcccataat gcctcaactt gtttttcttc tggcagttct gggaagactt
    57181 ttgctgccca ttgtttacca gccgcagcca cgacagtcca actgacttta tttgcttgag
    57241 ttgccttgcg taaattcatt agtgctttgc cattcgctga ttgataagac gctactcgat
    57301 ggctatctac accagcaaat gcatcgggat cggcagaaac aacgctgatc ctgcttgctc
    57361 ctttttcaag ccattcatcc gcttgatcga ttttagattg tggtacattc tcaatacgat
    57421 ccgttgcagc atgtaaaaga aattctcttt ggatctggtc gtcagtccat tgaacgataa
    57481 cctcagcggc tcctagttta taagcttctt gtgtgatcaa tcgagcaagg ggtgcctgtt
    57541 ccacgctgat ctgtaaaaca actgtgtgtc ctttttctgt ggctacgcca gtttctgcaa
    57601 ttaagcgtgc atattttttt agaagttcat taaaatcaga taacataata aaaattcctc
    57661 ctcaccattt tttctggtaa ctattagcat gataccattt aattatgatt caaacaatga
    57721 ggctttaaat aaaaaaagga ggttattttt ttaaggttaa gtttcatgat atatgttact
    57781 acttgctatt tcagcttgtg aagaaaatca aaaactaatt ttgcatatca aaaaaacatg
    57841 ttacaatgaa attaatcaat ggtggaagcg ttataaaaaa attaaggaga ttaaaaaata
    57901 tggcaagaaa aaagacgatt actagagaac agattttggc agctgcttat gaagtagctg
    57961 ccaccgatgg atttacacga tttactgcga gaaacatcgc gaacaaaatg aattgttcca
    58021 cgcaaccgat ttatcttgag tttaaaaata tggatgactt gagacaagct ctttttgagc
    58081 agatctatga tcatttacaa tatgaagtgt tcactgttga gcatacaggg aatacgatta
    58141 tcgatcttgc ttttaattat atccattttg caagaagaga gaagaagttg tatcgttcac
    58201 tgtacttaga agaatatggt ggaggaaaag agatgcagaa tttctcttat acttactttg
    58261 tgaatacagt gaaacgcgat ccagcttatg tgaatctttc agaagagcag atcgaatctt
    58321 tgtataatgg gacatggatc atagctactg ggatcgcagc tttgatgtct tcaagcatca
    58381 tccatccaac tgatgaacag attgaaaaga tgatccaaga cagtatagat gctatcttga
    58441 aattcaaaga accaatcgaa atcgattgaa tatactcaaa agttccacaa atcagtaggt
    58501 aacaagagat gtgtcaaaat aaggaaaagt tcgtttttct tacagcgtat gcatttttaa
    58561 ctcccgaaga actcttccat gaatgcaatg aataaaagag ccatacagat tgaataaatc
    58621 tggatggctc ttttattgca agtatattaa atttttgcga ctagttcttt agcgaaagct
    58681 tctaaatttt cgatatcttc ttcttcagca ttcagatcca ctttaacatt ttcggctcct
    58741 tttgatgcgc ctgtttttgc aaaagcagca tcgaaatcat ccaccgattt gcagaattca
    58801 tcgtaaaaag tatcaccaga tccgcagaca ccgtaaattt ttcccaatag atccaattct
    58861 tgcaagtctt cataaaagtc tacgatctca tcaggcagat cgccatcatc gtatgtataa
    58921 gtagctacta cgcaaatatc tgcctcctcg aattcatctg catctacttg ggtacattcg
    58981 ttgatttcga cttcgatatc cagattttca aatgcttctg caacaatatc ggcgatttct
    59041 tcagtgtttc ctgtcatgct agcatatacg atttttgcta aagccatgat gtgcctcctc
    59101 gtttataatt atttaacaat tatgaattat accaaaacat cattgaaaag aaaataaaac
    59161 ctaaagacac ctggggaatg tcatttggct aaatgtatgc cgctatgtta aaatgagagg
    59221 gatttgtaaa aaggagacga aaatatgatt actttaaaat cagcacgtga aattgaaatg
    59281 atggcagaat ctggagcttt attagcagat gtacataaaa atctgcgtga ttttataaaa
    59341 ccaggaatca caagttggga tattgaggta tttgtcagaa actatatcga gagtcatggc
    59401 ggaattgctg cgcaaattgg ctttgaaggg tatgaatatg cgacttgtat cagtataaat
    59461 gatgaaattt gtcatggatt ccctcgtaaa aagcctttga aaaacggtga tttggtaaaa
    59521 gtcgatatgt gtatcgattt gaaaggcggc atttctgatt cttgttgggc ctatgtagtt
    59581 ggagaatcta cacctgagat cgaccgattg atggaagtaa cgaagaaagc tttgtacatc
    59641 ggtattgagc aagcacaagt agggaatcgg attggtgaca tcggtcatgc tatccaaact
    59701 tatgtagaag gcgaaaatct agcgatcgtt cgtgatttta ttggtcatgg cgttggtcct
    59761 acgattcatg aagaaccagc agttcctcat tacggagaag ctggaaaagg gttgcggttg
    59821 aaagaaggca tggttatcac gatcgaacct atggttaata ccggaacttg gaaaatgaaa
    59881 atggatccta acggctggac tgcgtataca agagacggcg gactttcttg ccaatatgaa
    59941 catacattgg ctattactaa agatggtcca agaattttga catcacaagg agaagaaggc
    60001 acttattgat ctatccagtc aataggtacg ggagaggtac atgaaagttt ggaacaaaat
    60061 aaaacaaaat gaaaatctga tgcgctttat tgagacgact caaaagcgca tcactgattc
    60121 agaaatgagt acgacctctg tcgtcgttgc ttattattta ctcttgtctt tatttccatt
    60181 gattattgct tttggtaata ttttaccgtt tctccacatc gatcaagaga ctgtactgac
    60241 ctatttacga gaagtgattc ctgagaccgt ttaccagttt atcggtcctg caatcaaaga
    60301 ccttttgaca caaagttcag gaggcttgtt atccgtttct gcattagcgg ctttatggtc
    60361 tgcaagtcaa agtatcaatg ctttgcaaat tgcgatgaat aaagcgtatg gcgtagaaaa
    60421 tagaaaaaat tttttgatcg tccgtttctt ctcactaatt gtcattgtct tgttcatgat
    60481 tgctattagt ggggtaacta tcgttcttgg gttgggacag ctgattttgg atgctattca
    60541 gccaatcgta caaatacccg tgaactttat cgatcagttc caaactttga agtggccaat
    60601 cacattggta gctttatttg tcatcatgtt cttgatttat ttaattgtcc ctaatgctca
    60661 gttgaagttg aagacgatca ttccgggcac gatcttcgct actgtcggct ggatgctgtt
    60721 atcacaagtg tttagtatct atgctaggta ttttgcaacg aggatcagcg gttatcagat
    60781 cataggaagt tttattgtac tcatgctttg gcttaatttt gctgctacga tcatcatttt
    60841 gggcggaatc atcaatgcag ttgttcaaga atacgtgacg ggtcatcaaa tcaaaaaacg
    60901 tcgagatcca agccgaaaat ggttcaataa attgaaaact aaattttctc ataaaaaaaa
    60961 gtagaaacaa gagttcttat tgtaaattta tatcagtaat ttataataat tatgactctt
    61021 aattaaagta ggtgcccata tgcttgtttc ttttagtatg gttataagat tttttctgac
    61081 aattttatta tcactgatat taacacctgt attcaaaatc atttcagtac aaacaggtat
    61141 ggtcgacaaa ccaaatgaaa gaagaataaa taaagttcca atgccttcag cgggcggact
    61201 gcctatcttt atttcatttg ttatatcgac tttattctta ttccgtaaaa ttatccctca
    61261 atcgtatatt gtaccgatct tgattgcttc tttagtaatc atagtcacag gagtgctcga
    61321 tgataaatat gagctgacac ctaaacaaaa aagtataggg atcttgattg cttctcttat
    61381 tatttatttt gtagcagata tacgtattga ttccttcacg cttcctttta ttggatatat
    61441 ccaactaggc tggcttagtt ttcccgtaac gattttttgg atcttcggta tcacgaatgc
    61501 ggtcaatctg attgacggtt tggatgggct ggcggcaggg atttcattga tcggtttaat
    61561 tacgatagga atcattggat attttttcct ccatgcttct acagtgtata ttccagttgt
    61621 tattttctgt cttgtagcaa gtattatcgg tttttttcct tacaattttt atccggcaaa
    61681 gatttattta ggcgatacag gagctctatt cctagggttt atgatggccg tcctttcact
    61741 acaagggctg aagaacgtga cttttgtttc atcgatttct ttgctgatcg tcatgggagt
    61801 gccggtaaca gacacgtttt ttgctatcat taggagaaaa gcaaaccgtg tttctttttc
    61861 tacagcagat aaaaaacatc ttcatcaccg actaatcagt ttaggcttta ctcataaagg
    61921 tgctgtcctg acgatttatg cgatggcttt gatgttttct ttcacagcta tggtcatgaa
    61981 ttatactgga agaatcggaa cgatcgtatt gattattgcc atgttgtttg ctgcaatttt
    62041 attatttgaa ttgattggtc tgatcaatga gaaacatcag tttattttaa aaacattgcg
    62101 atttttaggg aataaagagt accgtacgca agtaaaacag aaatatttga agaaatggaa
    62161 ccgtcatata gatgaataac aaattaaacc agccgatcct tattagtatt gtgacggcaa
    62221 acagtgataa aatttttcgg acactagata attttatttc aactgtaaaa gatgaaacag
    62281 cagtaaaaat ccgaattttt gacaacaatt cacttcccga gtacaaaaaa agactaacag
    62341 agtataggaa atatccctac atcgatatct attttcatga tgaaaattcc ggatttggtt
    62401 atggacataa tcacaatctg atagctagcg attctcgcta tggcattatt tgtaacccag
    62461 atattttagt ggatgaagag acagttacag cattggtctt gttattgaaa gaacatccgg
    62521 aatgtgcgat gttagcacct aagattctta atgaagatgg aacaactcag cacctcatcc
    62581 gtgagcagct ggctgttttt gattacgttt tgcgctttgt gcctttcaaa tgggtcaaaa
    62641 aaaagtttga caaacgactg gctgtgtttg aatgccgagg attgcctact gatgctaata
    62701 gttatgtacg gatgatctca ggtagtttta tggtagctga tattgataaa tttaaagaga
    62761 taaaggggtt tgatgagcgt ttcttcatgt actttgaaga caacgacttg tgtttgagtt
    62821 ttgaaaaagc aggttacaag ttattgtaca caccctttta tgcagttatc catttttacg
    62881 ggaagggtgc gcatcgaaat gcaaaactgt ttcgcgtgtt tttgacttct atgatgaaat
    62941 ttttcaataa gtgggggtgg cgttttttct gatgaaacca gaaatattag tagtcgttgt
    63001 gctttatcag caactattca gtcaatctcc agcttatgga ccattaagca aagcacttag
    63061 cgaaaagcaa attcagctta ttatttacga taatagtcct caaaggcagc aaaatgagct
    63121 gtttgaaaaa gaaaatatcg tttatcatca tgatccgcaa aatcctggtt tagcggtggc
    63181 ttataatttt gcgttgtctc aagcagatga aggaacacgt tgtctcgtca ctctggatca
    63241 ggatacgagg ctagtcgacg attattttga actattgcgt aaagtaacct ttacagatga
    63301 atgtgttgct gcagttccga tgattttttc tgggagccga caagtttctc ccctatattc
    63361 agatcgttat atcaatcgta cagctcaagc agtagaagtt gaaggcccaa cagctgaacg
    63421 aatcatggca atcaattcag gtgttgcctg gtctgtcaaa tttctaaaaa agattggcgg
    63481 gtttaatcct gctttttctt tagatttttt ggatcattgg ctgttttgga aagtggatca
    63541 gttggaaaaa actgtggaga tccttcctgt acgaatggaa catgatcttt cggttttaga
    63601 ctatgaaaaa gtttcaagta atcgataccg ttctatttta caagcagaaa atcgatttta
    63661 tcatgaatat gatcaaaggc atttatccag tcaccgacga caattattgt taagaacagc
    63721 taaacagttc ttgaccgtca aaaaccggca tatctggcgg atgactttgc gctcatatgt
    63781 taagaattgg aaggtgtgaa ttcgtgcgtt cggtttgtat agctaccttt aatggaggca
    63841 gatatttaag agagcagtta gatagtattt tatctcagct ttcagcagaa gatgaagtag
    63901 tcatttctga tgatgggtca gtagatgata catgggatat attaactgaa tacgcagaaa
    63961 aagataagcg aatccagctt ttttcggggc ctcgtcaagg attgatcgct aattttggtt
    64021 atgcgatcca gcagacgaaa ggagatctaa tctttttggc agaccaagat gatgtttggt
    64081 tggagacaaa agtgaaaaag atcactgact attttgagca gaatccggat cagctagtag
    64141 tagtttctga tttaatcatt gtggacagcg agctgcaaga gatccatgat tcgtattttt
    64201 cgtttcgaaa ttccaaagaa ggatggctta ataatgtgat aagaagtcac tatattggtg
    64261 ctggtatggc tttccggtct tctttaaaac ccgatatttt gccgatcccg tcagaagtgc
    64321 cgatgcatga tatgtggatt ggtttgctgg gaggaaagaa gactgggttc ctaagagaac
    64381 cgttaactct gtaccgaaga catgatttaa acgcgagtga gattgaaaca cattctaaat
    64441 tacggcaaaa aatttcttgg cgtgttcatc tgattacggc gttgatcaaa cgaaaatggt
    64501 ttaaacgttg ataaaagatg tttaaatcct taatatttcc atgacaaata taacaattga
    64561 tgagataata acaaagtgaa atttgaaatc aaaaaggaga gcagatatcc atgaagggaa
    64621 tcattcttgc tgggggcagc ggaacacgtc tgtacccttt aacaaaggca acatcgaaac
    64681 aattgatgcc gatttatgac aaaccaatga tttattatcc aatgtctact ctgatgttgg
    64741 cgggaatcaa agaaattctg attatttcaa cgccacaaga tacaccacgt ttcaaggaat
    64801 tatttggaaa cggaaatgat ttaggactgc agatcgaata tgcagtacaa gaaagtccag
    64861 atggattggc acaagcgttt atcattggag aagaatttat tggagatgat agtgtctgct
    64921 tggtattagg ggacaatatt tactatggtg gcggtctgtc taaaatgttg caacgagcag
    64981 cttcaaaaga tacaggcgcg acagtatttg gctatcatgt caatgatcca gaacgctttg
    65041 gtgtagtaga atttgatgag aatatgcaag ctttgtcgat tgaagaaaag cctgctcagc
    65101 caaaatcaaa ttacgctgta actggccttt atttttatga caatgaggta atttctatcg
    65161 ctaaaggtat caaaccttca gaacgtggag aactagagat tacggatgtc aataaagcat
    65221 atttggataa aggaaaacta tccgtagaac ttatgggacg cggatttgct tggcttgata
    65281 caggaacaca tgaatcattg cttgaagcat ctacctttat cgaaacaatt gaaaaacgtc
    65341 agaacttaaa agtggcttgt ttggaagaaa ttgcttaccg tatgggctac atcgataagg
    65401 aacaattgct ggcccttgca cagccattga aaaaaaatca atacggtcaa tatttattga
    65461 atttggccgc tgaataagga gtccttacag atgaaagtaa cagatacaaa attaacagat
    65521 gtcaaaatcg tagaaatgga cgtctttggc gatcatcgcg gtttcttcac agaaagttat
    65581 tcaaaagcaa aatttgcaga acatggattg aaatatgact ttgttcaaga caaccattca
    65641 ctatcagctg aagctggtgt ccttcggggg cttcatttcc aaaaaggaga agctgcacag
    65701 acaaaattga ttcgggtagt cactggagca gttttagatg tagtagtgga tatccgcaaa
    65761 ggtagcccga cttatggtca atgggacgga tttatcttat ctgaacataa tcatcgacag
    65821 ttgcttgtac ctaaaggata tgcccatgga ttcgttactt tgacaccaaa tgtgaacttc
    65881 ctttataagt gtgataatta ctataatgca gaagcagatg ggggcatcgc atttgatgat
    65941 ccagatttag cgattgattg gccaattgat ccatcaagag cgattatgtc ggaaaaagat
    66001 caccgtcatc cgacattaaa agaatttgaa gcagaaaatc catttgttta cggagaaatc
    66061 taagaattaa ggagaaattt tcttatgaaa aacatcattg ttaccggagg tgccggattc
    66121 atcggctcta atttcgttca ttacgtggta aacaatcatc cagaagtgca tgtcactgtt
    66181 ttggataaac tgacttatgc aggaaacaaa gagaacttgg ccggacttcc tagcgatcgc
    66241 gtagaattgg tcgtaggaga tgttgcagat gcagaattag tggaccgtct agtaaaagaa
    66301 gcggacgcag ttgttcatta cgcggcagaa tcccataacg ataattcatt aaaagatcct
    66361 ttcccatttg tacaaacgaa tatcattggt acgtatacac ttttagaagc ttgccgcaag
    66421 tatgatgtac gctatcatca cgtatctact gacgaggttt acggcgatct tccattacgt
    66481 gaagacctgc caggacatgg ggaaggcgaa ggagaaaaat tcactgcgga aacaccatat
    66541 aatccttcaa gtccatattc ttctacaaaa gctggttcag atctgttagt aaaagcatgg
    66601 gtgcgttctt ttggcttacg tgcaaccatt tcaaactgtt caaacaacta tggtccttat
    66661 cagcatattg aaaaatttat tcctcgccaa gtaacgagta tcttaagcgg tattcgtcca
    66721 aaattatatg gcgaaggaaa gaatgttcgt gactggatcc atacgaatga tcattcttca
    66781 gcagtgtggc tgattttgac gaaaggcaga attggcgaaa cctatctgat cggtgcagac
    66841 ggtgaagaag ataataagac cgtgatggaa ttgatcttag aaatgatggg ccaaccaaaa
    66901 gacgcatacg accatgtaaa cgaccgcgca gggcacgatt tgcgttatgc aatcgattcg
    66961 acaaaattgc gagaagagct aggttggcag cctgagttta cgaacttccg tgatgggttg
    67021 gctgacacga tcaaatggta cgaagaacat gaagactggt ggaaaaaaga aaaagaggcc
    67081 gttgaagcag catatgccaa aaatggacaa taatacagag gaaatttcac tgtattaatg
    67141 gaaaaacaca tgtgaggcag gcgctcgcct gtctcttttt taaaataaaa atcaaggagc
    67201 ggataaccat atgtttttat taactggagg aaatggacaa ttaggtacag aattgcgcca
    67261 tttattagat gaaaaaaatc tgccatatgt ttcaacagat gcaaaagact tggatatcac
    67321 agatgctgag aaaacaatgg cttatattac agaattaaaa cccgatgtta ttttccattg
    67381 cgcagcttac acagcagtag ataaagcaga agaagaagca aaagaattag atgagaaaat
    67441 caatgtcgat ggcacaagaa atgtagcact tgcagcaaaa gctgtgaatg cacaattcgt
    67501 ctatatcagt acagactatg ttttcgatgg aaaaaacaaa aatgtagaat acgaagtaga
    67561 cgaccaaacg aatccactaa atgaatatgg tcgtacgaaa ttattaggcg aaaaagcagt
    67621 agaagagatt ttagaagact attatatcat acgaacttct tgggttttcg ggatctatgg
    67681 acacaatttc gtcttcacga tgcaacgcct agcggaaacc cgcgacaaac tgactgtagt
    67741 aaatgaccag tttgggcgac caacttggac acgtactttg gcagaattta tggtatatgt
    67801 catcgaagaa aaagcaccat ttggtattta tcatttgtcg aacgaaaata gctgttcttg
    67861 gtatgagttc gcaaaagaaa tattgaaaga tacagatgta gaagttgcac ctgttacgtc
    67921 agaagaattt ccgcaaaaag caacacgtcc gcagtattca gtcatgagcc tgaaaaagac
    67981 agaagcgtta ggttttgtca taccaacttg gcaagaagca cttgcgcaaa tgctggaagc
    68041 tgccaaaaaa atgaataagt aatttccttt ttatcaaata gaatttggag catgatatgg
    68101 aaaaacaaac aagaagtgca cgacgaaaag aaaagccaaa agcgaatcag gcagattcga
    68161 atttggtgct tgacctgttt ctaaaactcc gctatcttat tggcgtttta gtcatcgtgg
    68221 tcatcgttac ttttaattta aacggaagtt cacttggtgt ttgggataaa tatgtgtcgc
    68281 aaagagacga tgggaaaaaa actgatgtga tttttggaca gaatcgggaa gtgcggtcag
    68341 acgaatggct ggtgcagaca cctttttacc tctcacaagc tgaagaggac ttttcgttgg
    68401 tgaacgagga ttattcatta aacggacaaa acatgatcat tgcgtataat tctcctgtaa
    68461 aggatattac ggttatcgga aaaccattca actggggatt ctttttcctc ggaagagatc
    68521 gaggactttc cttttattgg gcgatgaaat tagtcgtcat ggtcttgctt ggattcgaag
    68581 tactgatgat attaacgaag aggaaaaaag ctctatcgct aatcggtgca tttgcgctga
    68641 cattttctcc agcagtccag tggtggttca tgcaacatgt gggcgatttg attttcttta
    68701 ctttgggact gatgattgct ttttatcatt atttctacaa tcatgataaa aaatggctac
    68761 gaacgttgat gatgcttttg gtggttattt tcggtattgg gtttatttta gtgatttacc
    68821 cagcccatca agtgatgctg gcgtacttgc tgatttttta ttttgttggc ctgcttattt
    68881 actatagcaa gaaagtttca tgggatctgt tggatgtagt gttgattgga ggggcagttt
    68941 tatttattgg cggagtcatg gttcatttct ggctaacgtc aaaagacgca ttgttagctt
    69001 ctttgaatac cttatatcca ggaaaacggg taagtactgg aggaaagtgg acgattggcg
    69061 aatttttttc tttccttaca aattggaaaa ttccttttga agatatcaac tattcgaata
    69121 actctgaagt ggccctgttc tatcactttt ttccaactgt attcctagca agtccatttg
    69181 tgttatttgg caaaaaagat agcgaacaaa agttactggg acgtattttg atgatttttt
    69241 gtctgttttc tatcttttgg attaccgttg ggttgccaaa atggctagca gaaatcactc
    69301 tgctgtctta tgttcctcca ttaagagcca tccttacttt tagttttgcg gcttgtttat
    69361 tatcgatctg gtttatcgct tatatttgga gagaagatgt tattcctatt tggtataaat
    69421 tcattttgtt ggcagtaaat gcatggtctt atttctatgc gattacgcat tcgaaaatgg
    69481 ataattattt ttcggatttt gaaaccatcg cagttgctgt gttgggaaca gtgattttag
    69541 cttttgcatt atttggctgg agacgcttgt tttacctcgc ttttgtcgcc ttagttatcg
    69601 tatctggctt tttcgtcaat ccagtcgtag aaggtacagg agcgattttt gaaaaaacac
    69661 tggcaaaaga aatccaaaaa atcgatcaac aagatcctgg taaagtttgg ttgtctgaag
    69721 atgaattata taacttcacc ccaacattgg gagtgaaatc cttcaataca gtacgctttt
    69781 atccagatat agctgcatgg aagaaaatcg atccgaaaga agaatacgag aagtattata
    69841 atcggtatgc tcatacaaga gcttttatcg ctaaggaaga tacaaagttc attttagatc
    69901 gtccagatat gttttcggtc acgttgaatt ttgaagacgc agaaaaatta gggatcgact
    69961 atgtgatcag taaacgacca ttaactgatt acaaccaatt atatgaagcg cagttcacgc
    70021 agctatatgg accggataaa gacggttata tgatttttaa agtagaatat ccggattatc
    70081 cagatgtacc acaaacacaa actcaggaaa atcagctagt cgtacagtaa taggctagct
    70141 gagaataatc ctgaaatatt agtagaaagg agagtgaaga taattcaagc aatcgtacaa
    70201 aaatttgtca acaaaatgag tgtcggtttc ttgcttttgt tgctgacgct agtctttttt
    70261 gtttggacag caggggtagg ttttccagca actttgttta tatataacaa tagttcatta
    70321 cttatcgctt ttagtgtggc tattttgctc attttaaatg tttataaagc aaaaggcgtc
    70381 gattttcttt atctaggtgt tgcgcttgcc cttttcattt tcttttctta ctttcgggaa
    70441 ttaagaaatg agtctttatt tggagactta ctgtttcctt tggttatttt attttgttta
    70501 gtaattaaat ggatcgagtg gacaaaatgg gatcgcggta tttatctgat tgtctttacg
    70561 gtcttcctcg gaatcacgtt atatcgtgta tttacagaga tccaagtacc agaaggacaa
    70621 agcatctggg ctccaggcaa ttcgctttct gatatttgga tcaatacgaa tacaatcgga
    70681 agttctttga tgactcttgc tttattgatc agtggatttg ccagttcttt tgaacgatgg
    70741 tatgttcgaa tcatcggtgg gatagcagtc gttgcagcct ttttaggtat ttgggtctgc
    70801 caatctagag gtgcattagt cgcgttgatt gtttttgcga tattggatct tttacctaaa
    70861 tcgttgatgc gtgtcattcg tgcgccattt attgcctaca cggttacgat tcttttagct
    70921 ttgccgattt cttatctggc ggctgtctct gaaaaagtca atttgtttac cgatcgagaa
    70981 gatatttggc ataaattcta tcagacgcta ggagaaaaat ctgaacaagt tttactagga
    71041 atgaaaacat ttatcttcca gcgaggaaac caatttttag gaaaccacaa tagttataat
    71101 tctattttga atatctatgg attgatagga tttgggattg ctgctttgct gcttatcctt
    71161 tttattggtc gcttaacgtt gaaagcagat ttatccaacg ggcagatgac ttttatctgg
    71221 gccttttttg cggtgatgat gcagtcattt atggaggata cattgacttc atacccatgg
    71281 gttccgatta tttatatcct gttggctatg gccgttcatc gatatgatga accagaagtt
    71341 gcggaggaat tctatgttat ggaagaggca tatgacgata tgacttatga tgatacatct
    71401 tatgaggaac aaccagatag cgctcattct tccaggatcc aacgctatca ttgataggca
    71461 caaaatacga gagaggctgt gacaaccgtc gcagcctttt ttggtttatt gcaattgtct
    71521 agcatcttct gtaaaataga aacgttagag aaaatcttgt atcatagaaa attaagaaag
    71581 aaataagaac gcagctcggt accaaaaata tggtaaaatg gcacttgact gtaattcact
    71641 gaaaatcgct tggaggaaca caaatgttta aagatttgta tttatttatg aaggacattt
    71701 atcagaacag aaaattattg ctacagtttt cgatcaatga cttcaaatca agatatgcag
    71761 gatcgttttt aggaatctta tgggcgttta tcaatccgct atttactgta ttgatttatt
    71821 ggctggtatt tggtgttgga ctgaaagcac aaatgatcga tgcaaaatac ccatttatcg
    71881 tatatttagt taccgggatg attccctggt tcttcttctc agatgcattt gcatcaacta
    71941 cggttgtttt tcgggaatat acttatttag taaagaaagt cgtattcaat attcgtatct
    72001 tgcccacgac gaaaatccta tccaatctgt acacccattt attcttcatt ctgattggca
    72061 tagctgtcgc gcttataaat ggtatatatc cttcagcaat gtctttacag ctgatttatt
    72121 atttattttg catgtgtgct ttcttgacag gattgacttg gctgacggca tcgatccaac
    72181 catttttacc tgatatcatg caattaatta cgatcgtgct gcagttgatc atgtggacat
    72241 taccgatatt atggagccct aatcagtttg attcaggtct tgtaagcata ttgaaattaa
    72301 atccgctgta ctatgttgtt caaggttatc gtgagtcatt tttaagtgaa gcttggttct
    72361 gggagcatgg aacatatact ttgtatttct gggttttcac gttagtaatg ttcgtaatcg
    72421 gctctgcagt gttccgtaga ttgaaaccac atttttcaga tgtgctgtaa ggagaatagc
    72481 tatgacagaa tatgcaatta gactaaaaga tatcacaaaa tcttataata tgtacgcaaa
    72541 accttcggat cgatttcgtg aagccctcaa tccttttaag aaatcctatc atgatgtatt
    72601 ttatgcgttg aaaaacgtga atgtagaaat aaaaaaaggt gagatgatcg gtttcgtggg
    72661 agaaaacgga tcgggaaaat ctacaatgtt gaaaatcatt acgggagtac tgacaccaac
    72721 atccggcacg atggaaatcg aaggaaaaat ctcagctttg ctagaactag gatcaggatt
    72781 caatcctgag tattctggct atgaaaatat ctatttaaat ggaatggttc taggattttc
    72841 aagagaagaa gtcgatgaaa tggtcgatga tatcattgaa tttgctgaca tcggagacca
    72901 tatttatcaa cctgtaaaaa cttattcaag cgggatgttc gttcgtctag cttttgctgt
    72961 agcaatcaat gtgaatcccg atattttgat tgtggatgaa gcattggcag taggcgatct
    73021 tgaatttcaa ctaaaatgta tggaaaaatt tacagaaatc aagaactcgg gaaagacgat
    73081 tttattcgtt tctcatgata tcagttcgat tcgtcgtttt tgtgatcgtt cctattggtt
    73141 gaaaaacgga gaagtcgtcg aatatggcga tacgatggaa gtaacgacga attatgaaaa
    73201 ctttttaaag aaaaaatcag ttaaaacagt ggatcggaat aaaaaccaag tagaacggtt
    73261 cgccgctcca gatatcgtcg acgtgacgaa agcggaattg ttggataaag acaaaaatcc
    73321 ggtggatatg ctggaacaag gagaaaaact atacgttaaa gtaacttatg aagtaaaaga
    73381 tgatacaatc aaaaatccgg ttttaggaat tgcgctacga acagtcgaca atttttatgt
    73441 atgcggactt aatactctac tagatggtat caagattcct tggaaaaaag gagagaatat
    73501 cttttatctg gagtatgaaa agttaggttt gttagctggt gaatattatt ttgacgttgc
    73561 ggtctttgaa gaaaatgcaa cggttccttt ggtatataag acaaagtaca tgaacttgtt
    73621 tgtcagcggt tcttatatcg gagaaggcat cgtggtctta gaccataaat gggaagaagg
    73681 gactcatagc aatgaaatat gattttgaaa tggacctaga tgaacaaagc agtgtgggaa
    73741 aaattgccgc acagatcaaa ccaggaagta aagttttaga gtttggtccc ggaaacggcc
    73801 gattaacgaa acatttaatc ggagcaaaac aatgtgaagt gagcattgtt gagttagata
    73861 aggaattgtt cgattttgtc agcgaatttg ctcaagatgg attttatggt gatatcgaaa
    73921 gttttgagtg ggcaaactat tatgctggac agacatttga ttacgtcttg tttgcggatg
    73981 ttttagaaca tttggtagat cctggaaaaa cattgaagaa agtcagagaa tttttgaatg
    74041 aagaaggaga gatcctgatt acattcccta acttggcgca taattctgtc atgatcgatt
    74101 tgttcaataa ccaattgccg tgggcttctt atggtctcct agatgaaacc cataattctt
    74161 tttatactca tgatggcttc caaaaagttt ttgaaaaagc gggattattc attaatattg
    74221 aagattattt gtacttagct gtaggtgata cagaattaaa atcaacttat gaagaattgc
    74281 cggaagctgt acgctatgat ttcaaaatgc gaccatttgg tgaagtctac caatactttt
    74341 tctcattgat gaaacatcca gtagcacaaa gttccattgc agagccacaa aattctaatt
    74401 atgtaaaagt gttagaagta acccagcaaa cgaaacaaga tgaaacaatc caacaaatac
    74461 cattcaataa tttcacaggg gaaaatgaga cactaagttt ccccgtatca gaaacaacag
    74521 aaagaatggt gtttcgcttc gcgcaacaac ctagctttat cgaattcagc gcagaagctg
    74581 ccggagaaaa actaacattt atcgattcga atgctgtagt caaaacagta aatgattgtt
    74641 atttgtttga tgggaaagaa ttaccagagt tcgttctaac ggatatttct gggaaagaag
    74701 tgactatcca ttgtcactat cgctttatcg gggaattgac accaacaatg aaagaattgt
    74761 tggaaacgat tcgtcccatg gctgaagtca caaagcagct ttctgagaaa aatgacgaat
    74821 tagaaagaga aaatcatcat cttcttgagg aaaatacaag actagaccat acgttaaaaa
    74881 caaccacaaa tcgctattgc acattgttgg aaagtgatga atggacaata aaacatagac
    74941 taggacgacg taagaaagaa acttcgaaaa aaatccaaga aaaagaactt tctatatgta
    75001 ttgacgagaa aatctgggat gcagaaacaa aaatattaaa aatcatcgga tggggaatcg
    75061 ctaattcgga tcgccagccc ttgtcctata agttatcagc tgaccaatcg ccatttttcg
    75121 aagcaattcc tgtttcacga gaagaagtca atcaagcaga acagctcgca aaaggaacag
    75181 aagcgggatt tgagttacga atcctttgcg agcaagaacg ttcattctta gtggaagcag
    75241 tagctcaaaa tggccaatca tggatcattg aaatgtaaat aatgattaac ttataaagat
    75301 aaggagtaaa cgaatcgtgc aaaaaaaaga aattgccgta tttatcgatc aaatcactcg
    75361 tgaacgggcc actggcgatt tgcttattgt gggctgggca attgatgaag tgaccaaaga
    75421 aatccctacg ataaaggtag aaaaagaaaa tgtgattgct gaagcaacac atgtcgtaag
    75481 attggatatc aatcatttat ataatcttga tgttaagacc caaagcggtt tcaaggtacg
    75541 tttatcaggg aaaatgagag gaaaagccat cttagacttc caaacagcga gacatcaaaa
    75601 tggcatagct gtgaaattaa acggtaaata cccttatgat gacggcattg aatcttcatg
    75661 ggaaagaaaa aaaagattac tgaaaaaagg gataaattat gcaaggactc atggcgtaaa
    75721 aaaagcgatc cgtcgggtca aattggaatt gaagccagga tcgatcgact atgcagagtg
    75781 gatcagtcgt catgagaaac cagatttgaa agagcaaagg aaagaagcac aaggatttgc
    75841 gtaccgtcca ttgatttcca tacttatgcc tgtctataac gttgaaatca aatggctcga
    75901 aaaatgcatt gattcggtac tggatcaaac gtatgatcat tgggaactat gtatcagtga
    75961 tgatgcttct actgatccag ctatccgaaa gtgtctggaa tcctatcaag caaaagatga
    76021 tagaatcaaa gtcgttttcc gaaaagaaaa tggacacatc agtctggcaa ctaattctgc
    76081 attggaaata gcagaaggag aatttatagc actcttagat aatgatgatg aattaccgcc
    76141 atttgcatta tatgaagtgg caaaagtgct aaacgtacat ccagaactgg atgtgatcta
    76201 cagtgacgaa gataaaatcg atgcagatgg caatcgcttt gatccgcatt tcaaagcaga
    76261 ctggtcgcct gatacattga tgggaaataa ttatatctct cacttaggcg tatatcgaac
    76321 aagcattgtt aaagaacttg gcggtttccg taaaggctat gaaggttctc aagattatga
    76381 tttggtcctt cgtgtgacag aacaaatccc tgcagatcat atttaccaca ttgaccgagt
    76441 cttgtatcat tggcggacga tcccaggatc aactgcaagt aatggcgaag ccaaaagtta
    76501 tatctatgat tccggagtga aagcattgac agatgccctg tctagaagaa acatcaaagg
    76561 aagcgtacgt cctggcagaa tttctggatt ttatgagatt gcttatgacg tcttgcaaga
    76621 ggatttggtc agtgtgatca ttccaacgaa gaatggctat gaagatctaa aaacttgtgt
    76681 ggattcgatc atcgaaaaaa cgtcttattc aaattatgaa atcattattg cggataacgg
    76741 aagtacagat ccaaaaatgc aagaattatt tgcagaatac aagcatcagc tgaaagaccg
    76801 ctttatcgta gaattgattg acattccatt caattattct cgaattaaca atttagcagc
    76861 agaaaaagca aacggcaaat actttttatt cttgaataac gatacagaag tgattgagcc
    76921 agattggatg acggtgatgg tttcttatgc acagtttgat cgaatcggct gtgtaggagc
    76981 aaaactgttt tatccagatg atacaacgca gcatgcagga gtattattag gaatcggtgg
    77041 tgttgcaggg catgcattga ataactatga tcgaacacat tgtggttatt tcggccgatt
    77101 ggtcattgac gtcaattact tagcagtcac tgcagcttgt atgatggtca aggcagctga
    77161 cttcaatgct gttaacgggt ttgatgaaac attagaagta gcattcaacg atgttgactt
    77221 gtgcttgaaa gtttatgaac tgggtcgtta caatgtctat gcacatcaag cagaattgta
    77281 ccatttcgaa tctaaatcaa gaggttatga agatactcca gaaaaacaaa gacgttttgc
    77341 tggtgaaatc aaaaaaatgc aagataaatg gccagcatat attgcgcatg atccatttta
    77401 caatgataat ttaacaaaag acggaatcgg cgacttttcg ttgagaccag attaaactat
    77461 aataaaaagc tgttatgttg tgattcgctc gcacataacg gctttttata gttttttgtc
    77521 aaaaaaaggc agaaggtgcc gactcaaaaa aataagtcga ggttgggaca gaagtgttta
    77581 actccgagaa ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcggc
    77641 ttatttaaga agaagttgct tctgctccca ccgtttattc gtttttaaag aatgagacaa
    77701 aagtgtttct tacttttgtc ccacgctctt attcatgctc agagcgccaa tagatacaaa
    77761 atcatagcca aagtttcacg aaccctagct gtcaaagaat cgatatttta ttttaatata
    77821 ggctatttta aaataaatgg tttaaggtat tcctctacaa aagccactct atcaattatc
    77881 atgggtttaa aataagaagt gatggagata accagattgt tcttagcgtc aatataaaga
    77941 acatttccac tattaccaat tgctgaatat atttcgttct cagaatctaa tacccaccat
    78001 aaatagccat atctcatatt ttgtaagtct tctaaagcac tggttctagg tttgaacatc
    78061 tcgtttatcc acatgggtgg tacgatttgt tttcctttat acatcccttt atttaggcat
    78121 aatacaccaa tttttgccag atcacttgcc gataaacaca aaccatatcc tgccgtcccg
    78181 atatttagtg gatcacaaaa ccagacattg gttttaggag acttatccat tgtaaactgc
    78241 ttatgttctt cagctgtttc agcataataa ttgacatggt ttggtatccc tataggatca
    78301 aatagatact tattggcaaa atcaacagtt gttagcttac ttgtctgata caacaagcca
    78361 gttaatatgt gtaaacagac agtttgatag ttgaattcgc cggttatacc ttttcttcct
    78421 cccaataaat ctagtgatgt ttttgtccaa tcttcacttg aacagacttt tgtccatgga
    78481 tcgcctttac atttgtaggg tgctttcatt gttagcaagt gcttgattgt aatagcgtaa
    78541 atagttttct ccccgcgttt cactttatac tctggaaaat aatctaatac tttatcatcg
    78601 atacttttga ttaatccttg ctctaaagca atgccaatca gcaaagatac aatacttttc
    78661 gttgctgaca tgatatgaca agtatcgtct tttttataac cattccattc atctgtataa
    78721 acttctaccc catccttata tgcgacaatt tgacatatat ttggttgtcg aaaaccaata
    78781 aaatcatgca actcttgttt attcataaga tattacctca ctttcttcta taagcataat
    78841 gtcattaaaa atcgatttca attttttcga ttcttcacgt aatacaggta ttcttatgga
    78901 ctagacagcg ctttgggaag atggattctc tgaaaaatat aaaggttcac tttatgagtg
    78961 gataaaaaaa gcagcaaata cttcgacttc tttaatttac caacattagt gaaaatcgat
    79021 tcatctggag atttgtaatt tagagtcctt gtcttgatta gaggtgtata aagcttttta
    79081 ttagacattt ttaaattcca ttacaatttg cttataaact aggtttttaa aggtgactat
    79141 ggtaagataa tagagatatt attttgtgat taggagtgta tatatgaata aaaatggaga
    79201 atggagtgct gcacgacgga tattcattat attaatggat gttattgtat acaatgcagc
    79261 aatatatagt agtttcttac tgaagtttcg tggagagatc ccattacgaa atttagaaac
    79321 tttccaacac tcagcaatta ttatttcagt gatttttatt gcgttgaaca ttttgttggg
    79381 tgcatatgtt ttttataatc gaatgatcag tgacatcgtg tttgtcacag tcatcggtca
    79441 ggtattgatg acactaggga tcatggtagt gacttttgcg ggccgctggt ttgcttttcc
    79501 acgtcttgtt atcttacttt catttgttct cggtacgctt ttcttaagta tctatcgtat
    79561 tctcgtatat aaactttatt tacgcgtaag tcgagataaa aaggtcgtca ttattgggtt
    79621 agaaaaggat gtcgcaccag caatccaaaa ttttggttca aagaaaaaca acaaacataa
    79681 agtcgaagca gtcattcttg ataactatta tgcaaacatc aaaaatatga ttaaagacat
    79741 cgatattgtc tatcttgctt ctcatatcga agaaactgaa aaaatcaaaa tctatcaatt
    79801 agttactaaa aatgaaaaaa aattgttttt gaatacaaca tttgaaaact tgacattgat
    79861 caatccaaat attatgaatt tcgaagatga aagtattatt gaagcatctg gatttagaat
    79921 ccaacctgaa tatgaactat tcaaacgttt gtttgatatc atcgtttcac ttgttttatt
    79981 agttattgct tcaccattta tgttattcac agcaatttta gtcaaagtga catcaccggg
    80041 accaatcatc tataaacaag tgcggatcac aaaaaatcaa aaggaatttt caatctataa
    80101 attccgctcg atgaccgcca cagccgaagc aaaatctgga ccagttttag ctaagagcaa
    80161 tgatgcacga gtaacgcctg taggtaaatt tattcgagct gtccgctttg atgaattacc
    80221 acaaatcttc aatgtgctga aaggcgatat gtcgatcgta ggaccacgtc ctgaacgttc
    80281 tttcttcgtc gatcagttca atgcagaaaa cccttattac tacttacggc ataatgttcg
    80341 tgctggtatc actgggtatg cacaagtcta tggaaaatat gtctctgatt acaacagtaa
    80401 attacgtttt gatttacttt atatcaaaaa atattcattg atgcttgatt taaagatctt
    80461 gctgcaaacc atcaagatcc tatttgataa aatgtcctct caaggacttg aagaagacga
    80521 aaatgtgatc tcattggcag aaattgcttt tccgaatgaa aaaatctttc gctagttggg
    80581 aggcgtaaaa atgaacaatc cgaaattcag cataatcgta ccagtataca atgcagaagc
    80641 aactattggt cgaacgattg atattctaaa agaattggat tatgaagagt acgaagtcgt
    80701 tttagtaaat gatggatcaa cagatcaaac acaggaaatc attcaagaac tgattgaaaa
    80761 tgatgaacgt tttgaattga ttactacgac caatcaaggg cccggattag caagaaacga
    80821 aggaataaga cagtcaaaag gagaatatct cttattcttc gatgcagatg acacgccagt
    80881 aaaaacgatc ttgtcagatt atgatcattt gctagaaaag agtccaaaag cggatcttgt
    80941 tgttagttcg tttgtttttc gtacaatgga cgagggaaga atcgtttccg aaaaagaaaa
    81001 cttagttaca gaatgtcact atctaactca cgaagagttt ataaaagaca tgtatcagtt
    81061 aatgaatgaa cagttgatgt atgttgtatg gaacaaatgc tatcgtagcg atattctgaa
    81121 aaataaccaa attctattta aaacttataa tagttgtgag gatcgaatat ttaacttaca
    81181 ttattatgaa tactgtcatc aagtgatcat gaacccaaaa gtagaatata tttatgaatt
    81241 tgaaggtggg aaggggatca ctaatcaata ccgacctaac aagttcgaaa catttaaaga
    81301 attctattca ttagcaaata ctgtaacaga cgatattaat aaagacggta tggcggcgct
    81361 tcttttaaaa gggacaactt cagttatttt ttctatttat agtacctctg caagaacttc
    81421 aaaagagaag aaagacgaag caaagcagat attgagtgat ccagtgatcc gagaagccaa
    81481 gcaaattgct aagacggatt cttcagcaaa aaaaataaca aagttgcttt acaatatgcc
    81541 agctccacta tttttatctg ctgtaaaagt tggaagcttc gttgaagtga aaatgccagg
    81601 gttaatggct gctttgaagc gagtatatta gttaatggag gaaaatataa tgaaaagaat
    81661 tttgcatttt cagggacgaa tgggtttagg tggagcagag tctttcatga tgaatttata
    81721 tcgtaaaatt gatcgtacaa actatcaatt tgattttttg atttatgaag attatgcaga
    81781 tgttcaagac tatcattcag agattgaaag attag
//
LOCUS       DAZULO010000003        76368 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000003 DAZULO010000000
VERSION     DAZULO010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.003"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1058
                     /locus_tag="ACG8OA_000176"
     CDS             <1..1058
                     /locus_tag="ACG8OA_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFS4975025.1"
                     /translation="NLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVGK
                     ILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKG
                     DKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLK
                     MEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWL
                     FQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAV
                     LTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLT
                     PVWIVVLQMIHGIGLFS"
     gene            1077..2096
                     /locus_tag="ACG8OA_000177"
     CDS             1077..2096
                     /locus_tag="ACG8OA_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFS4975026.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(2198..2914)
                     /locus_tag="ACG8OA_000178"
     CDS             complement(2198..2914)
                     /locus_tag="ACG8OA_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4975027.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(2883..3668)
                     /locus_tag="ACG8OA_000179"
     CDS             complement(2883..3668)
                     /locus_tag="ACG8OA_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4975028.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(3659..4408)
                     /locus_tag="ACG8OA_000180"
     CDS             complement(3659..4408)
                     /locus_tag="ACG8OA_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS4975029.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4706..5230
                     /locus_tag="ACG8OA_000181"
     CDS             4706..5230
                     /locus_tag="ACG8OA_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFS4975030.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5246..6745
                     /locus_tag="ACG8OA_000182"
     CDS             5246..6745
                     /locus_tag="ACG8OA_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFS4975031.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(6833..7435)
                     /locus_tag="ACG8OA_000183"
     CDS             complement(6833..7435)
                     /locus_tag="ACG8OA_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4975032.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7539..8495
                     /locus_tag="ACG8OA_000184"
     CDS             7539..8495
                     /locus_tag="ACG8OA_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFS4975033.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(8800..9756)
                     /locus_tag="ACG8OA_000185"
     CDS             complement(8800..9756)
                     /locus_tag="ACG8OA_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4975034.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            9930..10490
                     /locus_tag="ACG8OA_000186"
     CDS             9930..10490
                     /locus_tag="ACG8OA_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975035.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10483..11307
                     /locus_tag="ACG8OA_000187"
     CDS             10483..11307
                     /locus_tag="ACG8OA_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFS4975036.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11317..11934
                     /locus_tag="ACG8OA_000188"
     CDS             11317..11934
                     /locus_tag="ACG8OA_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS4975037.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            11952..12635
                     /locus_tag="ACG8OA_000189"
     CDS             11952..12635
                     /locus_tag="ACG8OA_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFS4975038.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13318..14553
                     /locus_tag="ACG8OA_000190"
                     /pseudo
     CDS             13318..14553
                     /locus_tag="ACG8OA_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            14561..15448
                     /locus_tag="ACG8OA_000191"
     CDS             14561..15448
                     /locus_tag="ACG8OA_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4975039.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15465..16301
                     /locus_tag="ACG8OA_000192"
     CDS             15465..16301
                     /locus_tag="ACG8OA_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4975040.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16304..16927
                     /locus_tag="ACG8OA_000193"
     CDS             16304..16927
                     /locus_tag="ACG8OA_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975041.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            16924..18216
                     /locus_tag="ACG8OA_000194"
     CDS             16924..18216
                     /locus_tag="ACG8OA_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4975042.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18575..18672
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            18788..19369
                     /locus_tag="ACG8OA_000195"
     CDS             18788..19369
                     /locus_tag="ACG8OA_000195"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFS4975043.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19372..20679
                     /locus_tag="ACG8OA_000196"
     CDS             19372..20679
                     /locus_tag="ACG8OA_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFS4975044.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            20972..21460
                     /gene="purE"
                     /locus_tag="ACG8OA_000197"
     CDS             20972..21460
                     /gene="purE"
                     /locus_tag="ACG8OA_000197"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFS4975045.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21453..22577
                     /gene="purK"
                     /locus_tag="ACG8OA_000198"
     CDS             21453..22577
                     /gene="purK"
                     /locus_tag="ACG8OA_000198"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFS4975046.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22590..23885
                     /gene="purB"
                     /locus_tag="ACG8OA_000199"
     CDS             22590..23885
                     /gene="purB"
                     /locus_tag="ACG8OA_000199"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFS4975047.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24065..24442)
                     /locus_tag="ACG8OA_000200"
     CDS             complement(24065..24442)
                     /locus_tag="ACG8OA_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975048.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            24698..25426
                     /gene="purC"
                     /locus_tag="ACG8OA_000201"
     CDS             24698..25426
                     /gene="purC"
                     /locus_tag="ACG8OA_000201"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFS4975049.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            25426..25680
                     /gene="purS"
                     /locus_tag="ACG8OA_000202"
     CDS             25426..25680
                     /gene="purS"
                     /locus_tag="ACG8OA_000202"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFS4975050.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            25682..26353
                     /gene="purQ"
                     /locus_tag="ACG8OA_000203"
     CDS             25682..26353
                     /gene="purQ"
                     /locus_tag="ACG8OA_000203"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFS4975051.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            26354..28576
                     /gene="purL"
                     /locus_tag="ACG8OA_000204"
     CDS             26354..28576
                     /gene="purL"
                     /locus_tag="ACG8OA_000204"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFS4975052.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            28561..30000
                     /gene="purF"
                     /locus_tag="ACG8OA_000205"
     CDS             28561..30000
                     /gene="purF"
                     /locus_tag="ACG8OA_000205"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFS4975053.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            30023..31075
                     /gene="purM"
                     /locus_tag="ACG8OA_000206"
     CDS             30023..31075
                     /gene="purM"
                     /locus_tag="ACG8OA_000206"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFS4975054.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            31072..31650
                     /gene="purN"
                     /locus_tag="ACG8OA_000207"
     CDS             31072..31650
                     /gene="purN"
                     /locus_tag="ACG8OA_000207"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFS4975055.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            31647..33182
                     /gene="purH"
                     /locus_tag="ACG8OA_000208"
     CDS             31647..33182
                     /gene="purH"
                     /locus_tag="ACG8OA_000208"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFS4975056.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            33198..34472
                     /gene="purD"
                     /locus_tag="ACG8OA_000209"
     CDS             33198..34472
                     /gene="purD"
                     /locus_tag="ACG8OA_000209"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFS4975057.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            34594..35508
                     /locus_tag="ACG8OA_000210"
     CDS             34594..35508
                     /locus_tag="ACG8OA_000210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFS4975058.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(35641..37206)
                     /locus_tag="ACG8OA_000211"
     CDS             complement(35641..37206)
                     /locus_tag="ACG8OA_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4975059.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(37199..38650)
                     /locus_tag="ACG8OA_000212"
     CDS             complement(37199..38650)
                     /locus_tag="ACG8OA_000212"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4975060.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(38664..39680)
                     /locus_tag="ACG8OA_000213"
     CDS             complement(38664..39680)
                     /locus_tag="ACG8OA_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4975061.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            39900..40988
                     /gene="chvE"
                     /locus_tag="ACG8OA_000214"
     CDS             39900..40988
                     /gene="chvE"
                     /locus_tag="ACG8OA_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4975062.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            41042..42568
                     /gene="mmsA"
                     /locus_tag="ACG8OA_000215"
     CDS             41042..42568
                     /gene="mmsA"
                     /locus_tag="ACG8OA_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4975063.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            42570..43775
                     /gene="mmsB"
                     /locus_tag="ACG8OA_000216"
     CDS             42570..43775
                     /gene="mmsB"
                     /locus_tag="ACG8OA_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFS4975064.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            44032..45009
                     /gene="guaC"
                     /locus_tag="ACG8OA_000217"
     CDS             44032..45009
                     /gene="guaC"
                     /locus_tag="ACG8OA_000217"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFS4975065.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            45273..46697
                     /locus_tag="ACG8OA_000218"
     CDS             45273..46697
                     /locus_tag="ACG8OA_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS4975066.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(46739..46996)
                     /locus_tag="ACG8OA_000219"
     CDS             complement(46739..46996)
                     /locus_tag="ACG8OA_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975067.1"
                     /translation="MPIRILTALVNKITIRKAFYFSSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            47158..48267
                     /gene="pdhA"
                     /locus_tag="ACG8OA_000220"
     CDS             47158..48267
                     /gene="pdhA"
                     /locus_tag="ACG8OA_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFS4975068.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            48270..49247
                     /locus_tag="ACG8OA_000221"
     CDS             48270..49247
                     /locus_tag="ACG8OA_000221"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFS4975069.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            49272..50915
                     /locus_tag="ACG8OA_000222"
     CDS             49272..50915
                     /locus_tag="ACG8OA_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFS4975070.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            50923..52329
                     /gene="lpdA"
                     /locus_tag="ACG8OA_000223"
     CDS             50923..52329
                     /gene="lpdA"
                     /locus_tag="ACG8OA_000223"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFS4975071.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            52491..52766
                     /locus_tag="ACG8OA_000224"
     CDS             52491..52766
                     /locus_tag="ACG8OA_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFS4975072.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            52772..53539
                     /locus_tag="ACG8OA_000225"
     CDS             52772..53539
                     /locus_tag="ACG8OA_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFS4975073.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            53707..55536
                     /gene="typA"
                     /locus_tag="ACG8OA_000226"
     CDS             53707..55536
                     /gene="typA"
                     /locus_tag="ACG8OA_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFS4975074.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            55797..57569
                     /gene="recQ"
                     /locus_tag="ACG8OA_000227"
     CDS             55797..57569
                     /gene="recQ"
                     /locus_tag="ACG8OA_000227"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFS4975075.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            57683..57985
                     /locus_tag="ACG8OA_000228"
     CDS             57683..57985
                     /locus_tag="ACG8OA_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFS4975076.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            57988..59151
                     /locus_tag="ACG8OA_000229"
     CDS             57988..59151
                     /locus_tag="ACG8OA_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS4975077.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            59177..62605
                     /locus_tag="ACG8OA_000230"
     CDS             59177..62605
                     /locus_tag="ACG8OA_000230"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFS4975078.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            62626..63750
                     /locus_tag="ACG8OA_000231"
     CDS             62626..63750
                     /locus_tag="ACG8OA_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFS4975079.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            63731..64177
                     /locus_tag="ACG8OA_000232"
     CDS             63731..64177
                     /locus_tag="ACG8OA_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS4975080.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            64161..64499
                     /locus_tag="ACG8OA_000233"
     CDS             64161..64499
                     /locus_tag="ACG8OA_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFS4975081.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            64618..65178
                     /gene="rsmD"
                     /locus_tag="ACG8OA_000234"
     CDS             64618..65178
                     /gene="rsmD"
                     /locus_tag="ACG8OA_000234"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFS4975082.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            65175..65666
                     /gene="coaD"
                     /locus_tag="ACG8OA_000235"
     CDS             65175..65666
                     /gene="coaD"
                     /locus_tag="ACG8OA_000235"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFS4975083.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            65656..66699
                     /locus_tag="ACG8OA_000236"
     CDS             65656..66699
                     /locus_tag="ACG8OA_000236"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFS4975084.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            66858..67586
                     /locus_tag="ACG8OA_000237"
     CDS             66858..67586
                     /locus_tag="ACG8OA_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFS4975085.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            67702..67947
                     /locus_tag="ACG8OA_000238"
     CDS             67702..67947
                     /locus_tag="ACG8OA_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS4975086.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            67944..68162
                     /locus_tag="ACG8OA_000239"
     CDS             67944..68162
                     /locus_tag="ACG8OA_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFS4975087.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            68298..68837
                     /locus_tag="ACG8OA_000240"
     CDS             68298..68837
                     /locus_tag="ACG8OA_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFS4975088.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            68830..71103
                     /locus_tag="ACG8OA_000241"
     CDS             68830..71103
                     /locus_tag="ACG8OA_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFS4975089.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            71141..72187
                     /gene="holA"
                     /locus_tag="ACG8OA_000242"
     CDS             71141..72187
                     /gene="holA"
                     /locus_tag="ACG8OA_000242"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFS4975090.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(72276..72530)
                     /gene="rpsT"
                     /locus_tag="ACG8OA_000243"
     CDS             complement(72276..72530)
                     /gene="rpsT"
                     /locus_tag="ACG8OA_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFS4975091.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(72930..73199)
                     /locus_tag="ACG8OA_000244"
     CDS             complement(72930..73199)
                     /locus_tag="ACG8OA_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975092.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(73461..73589)
                     /locus_tag="ACG8OA_000245"
     CDS             complement(73461..73589)
                     /locus_tag="ACG8OA_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975093.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(73669..74124)
                     /locus_tag="ACG8OA_000246"
     CDS             complement(73669..74124)
                     /locus_tag="ACG8OA_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFS4975094.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(74262..76190)
                     /locus_tag="ACG8OA_000247"
     CDS             complement(74262..76190)
                     /locus_tag="ACG8OA_000247"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4975095.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        24739 a        13195 c        16582 g        21852 t
ORIGIN      
        1 tgaacttatt tgacattttt cgatcgactt tgacagatat gtcgattgtc agtgcaatca
       61 cttcaacagt ttttattata ttattagggt ttgtttgtag aaaaaagggg attttttctg
      121 aatctgttgg aaaaattcta tcgaaagtcg tcttaacagt ggctttgcca gcactggcat
      181 ttcgagcttt catgcaagat atcaatacag aattactgat gcaaggaatc aatgtcttga
      241 tttggggatt agttatttat attattctta ttctagtttc aaaacctttt ttcttaagct
      301 ataaagggga taagcaaaca acgttgcgtg tcctgacgat ctttggttct acgacctttt
      361 tcggaacgcc tatcgtcagt gcgatttatg gtcctgtcgg cgtgatgtat tcctcaattt
      421 tcaatattgg ctaccgaatc tttttgtatt cttatggata cataaaaatg agtggcttga
      481 agatggaagc taaaaatatc aaagcgatgt tcgtcaatcc aatcattatc gctacttttg
      541 ccggcctttt catttggctg ttccaagggt atctgccaca agtgtcagtt acaggtgcag
      601 aaggagagat aagccaagta gcgtttttgc gaattgacca gactgcaccg tggctatttc
      661 agccgatgga ttatttagct ggtttaagtt caccattggc gtggctagca atcggcgcaa
      721 cattaggtga agtaagcttc aaaaaagctg catctgaaaa aacttcatgg tattacagtc
      781 tagtcaaagt ggtatttgta cctgcattga atatcgtgat tctagcagtt ttgacggcaa
      841 cgaatattct accagtcaat tttgaagcac tggcaacaat cgttatcatg atggctacac
      901 cagctgcaac agtggcagca gcctatgcta tcagttttga tagggaggca cttttagcct
      961 ccaatgcatc cttactgtca actatttttg ctgtggtact gacacctgta tggatcgttg
     1021 tattacaaat gattcatggc ataggtttat tttcataaga aatggaggaa gaaagtatga
     1081 gaatcgcatg ttacggtgtt cgtccgaacg agatagattt ttttgaaaaa ttgaatattt
     1141 accattatga tctcagtctt tatgaagaac tgttgacaca tgacaatatc gtgtcagcaa
     1201 aggaccatga tgctgtattg ttgaggggaa attgtatagc agatgagacc aatcttgcaa
     1261 aaatgcagga atatgggatt cgttatgttt ttacacggac tgtgggagtc aatcatatcg
     1321 atctacaagc ggcagctgat tttggtatga cagtagcccg tgtaccatcg tattcgccaa
     1381 atgcgatagc agagttatct ttgacttttg ccatgatgct tttgagaaat actgcgtata
     1441 cgacgatccg aacatctttc aaagattttc gagtggatga gcaaatgttt agtcgtgaaa
     1501 tcagaaattg cacagtaggg atcataggaa caggcaggat agggttgacg gaagctgttt
     1561 tgtttaaggg actaggagcc aaagtgatcg gctacgatat tcatcagaca gaagctgcta
     1621 aaaaagtatt gacgttccac tcgcttgaag agttattagc caaaagcgat attgtcagta
     1681 ttcatgtacc tcatatccca ggtgaaaacg atcaaatgat caatgaagga tttttagctc
     1741 aaatgaaaaa aggtagcatc ttgattaata cagcccgtgg agagcttcaa gataatgaag
     1801 ctattttaag agctttgaaa agcaatcatt tagctggttt tgcaaccgat gtttttgcaa
     1861 atgaaacaga ggtttttttc cgatccttca agccatggga gacgattcca gaccctgcta
     1921 tccagcaatt agtagaaatg taccctagag ttcttgtaac cccgcatgta ggttcaaata
     1981 cagatgaggc attatcaaat atgatttcga caagtttcga gaactttcgt gaaataatag
     2041 aaactggaaa aacgaaaaat gaggtatctc ttcctaaagc ccgtcaatta agataagaaa
     2101 acatggatat tcgtcaataa aatgtttaaa aatagtccaa gtaaacagtt gttacttctg
     2161 tatacttgga ctattttgta tgggataatt cgatgactca acagtaatct gtgagctttt
     2221 tccgcccatt ctttgtcaat tcataacagg ttagcggctg gccgatttcc aaatatttga
     2281 ttctttcgtt caatatatct tgttctacta aaaaattcaa atatttcttg acggatatac
     2341 gtgacagtga gcttttttga gccagttctt ttgttgaaaa aatagtatct tcttttagaa
     2401 taaatgcact tatttttttt aaagtcagtt ttgacagacc tttaggcaaa tcgatgaatg
     2461 atggtatttc gtgaatggga gtggtgtggt tgaatacttg atcgatcaat ttctgatcgg
     2521 ctttttcagt cgtttggatt gtctgagaga aagcaagata cttttcgatc gccagctgaa
     2581 aacgttgcaa agtgaacggt ttgatcaaat aatctaaggc accgtagcgg atcacattct
     2641 ttatggtttc gatatcatct gcagcagtga tcaaaatcac tgggatatca tttccttgct
     2701 gtctaagcca agataaaaaa tggataccac tttcgccagg catgtataca tcaagcagca
     2761 gcagatctgc gtttttttgt ctgaaatatt tttttccttc ttctacattt ctgaaacttt
     2821 cgattacgtt catattaggg atgctttcca caaaagtacg attcaattct gcaaccattg
     2881 gatcatcttc gattatcagg acatttgtca tcattttgat cttccttttt tggtaattct
     2941 agatagacga ttgtaccgcc atttgtttgt ttggaaaatt ctaaaaatcc accgtggcgt
     3001 ttgacgattg aatggatgag attcaatcca aacccacgat tgattccctt cgtcgaaaaa
     3061 cctagcgtaa atattttttc tgagatttct tctgatatcc cgacaccgcc atcgcagacg
     3121 gtcacgatga atacttcagc ttctgtgtca tagtttaaat gaatccggac ctgtccgttt
     3181 ctttcatgtt tttgtagcac ggcttctttt gcattatcaa gtaagttccc taacacttgc
     3241 aaaagtgaat ggatggagtc atccatctcc attttaggga taaaagaatc gctttctaac
     3301 agcatttcaa cagattgttc tttggcttcg tttattttgc ctaataaaaa accggcaatt
     3361 gcaggtactt tgatcagact tgagacttgg ccaatctctt ttttataatt ttgatttagc
     3421 tgctggataa aatgggtgac ttcttcatac ttcttcaaat caatcaaacc agaaatgaca
     3481 tgaagtttat tcatgaattc atgggtttga gctctcatgg agtttatata ttgtttcgtc
     3541 cccccaagct cctccattaa tttcattact tctgatcgat ttcgtaaagt tgcgactgct
     3601 cctaaaaaac ggtcattgct aaaaagaggt gtaacgctag aaatagtttc tatcccattt
     3661 aaatacatgg attgagtccg aacggtcgtt tgatgctcaa tcgtctcttt aaacaaaacg
     3721 tcatatagat tatcactgat ttttttccta cttcgaaatc caatagctgc tctgtttggt
     3781 agaaaatgtt ttttgcaggt gtattgatca agaggatagt tttatcagaa gagatggcta
     3841 agattccttc atctacttca ttttcgataa tcgttttttc acttaactgt tctgctattt
     3901 cttgtggttc aagtccaagc agtacttttt tcattttttg ggcaagaaaa acggcgccta
     3961 tgatgcctac gctaagtcca ataatcaatg aaaaaacaat cattttttgt ccatctttga
     4021 cttgattgtc aatcgtgttg tttgttaagc caacacaaac tgcaccaata atctcttgat
     4081 cataataaat agggacgaaa tagcggaagc cgaaccctaa cattccttct ttttcagaga
     4141 aaaattcttg tcctccaatc acttttttca catcttcatg acttgagaaa ggaagaccga
     4201 tgtaacttgg gtctgcgtga gaatatcgaa tgccttcagg gtccatgaca acgataaaat
     4261 ctaaattcga tgcaatcatg atttcatttg ttattccttg aatcgcatgt gtcgggtcac
     4321 cagtctgcag ctttttttgg atgagtttat cattggcaac gattttagca atgctagaga
     4381 ttttatctga cacactagat cggttcatcg aaagaataaa attcctgatc aatatggcag
     4441 aaacaatcaa agaaacgaat gtggagaaac aaacgacaag gatgatcgtg ttctggagct
     4501 ttaatggttt tttcttaaaa aaaggaaaca taggctacct cgaaagaatt tattgtgaaa
     4561 attttaacat gaaaacaaaa atttaacaaa ttgtcaacga taaacacaac cttcctataa
     4621 aaaacaccac tgagtcggta aaagaagcta tacagaattt tcaaacattg gtatgataga
     4681 cgtagaaatg gaaggaggcg tgtatatgat agggggaatc gcacttgaga cgatttattg
     4741 gtatacattg ctgatttgtg caggattggc agtcctattg attatttttg gagatgtatt
     4801 cgattttgat ggcccgcttg atccgatgct tattatccct tggttaactt tcacaagctt
     4861 atttggtttt ctaggtgagc gtttaacaga tcaatcatct ttatttttat ttattgctag
     4921 tggaggcata tcgatagtgt tggtattttt gcttaatttc tatatattga tgccgatgaa
     4981 acatgccgaa tcaacgatgt cgtcttctga aaaaactctg gaaggacaag tagctgttgt
     5041 gatcacaccg atccctgtac gaggtatggg agaaattcag ttgaaaagtg ttaccggttc
     5101 cattagtcgt ccagcttgtt tttacagccc ccaagaacag acagctgagc ggggaacgaa
     5161 agtattgatc attgagatca aggaaagaat ctgttatgtc atgccttatg aaagcatgat
     5221 aaaagcttaa aggagcgtgt gttaaatgga aataccagtt gtttttatcg cgatcgcaat
     5281 cgcagctttg attttactga tgttgctgat cgtctttgta gcgaagtacc agacagcaaa
     5341 accggatgaa gcgttgatca tcagtggaag ttatttagga agcaaaaatg tgcacattga
     5401 tgaaagcaat aacaaaatca aaattgtgcg tggaggtggc gcattcgtct tgccagtttt
     5461 tcaacgttcg aatcggatca gtctattatc gagtaaattg gacgtctcca cccctgaagt
     5521 gtataccgaa caaggcgtac cggtgatgtg tgatggtact tcgatcatca aaatcggttc
     5581 atctgtagaa gagattgcta cagcagcaga acagttttta ggcaagacaa gagaagaact
     5641 ggaaaatgaa gcaagagaag tattagaagg acacttgcgc tcgattcttg gttctatgac
     5701 tgtagaagaa atctatcaaa atcgcgataa gttcagtcaa agcgtacaag aagtcgcttc
     5761 tgtagacctt gcaaaaatgg ggttgatcat cgtttctttc actatcaaag aggtcagaga
     5821 caaaaacggt tatcttgact ctttagggaa accaagaatc gcccaagtca aaagagatgc
     5881 agatatagca gaagcggaag ctctaaaaga aacgcgtatc aaaaaagctc aagcagaaaa
     5941 agaatcacaa gcagctgaat tacagcgaca aacagagatc gcagaatcat taaaagaaaa
     6001 agaactgaaa ttagcaacct acaaacaaga acaagatgtt gctaaagcaa aagcagacca
     6061 agcctacaat ttagaaagtg cccgagcaca acaacaagta atcgaacagg aaatgcagat
     6121 taaaatcgta gaacgccaaa aacaaatcga actggaagaa aaagaaatca cacgtcgtga
     6181 aaagcaatat gattcggaag tgaagaaaaa agcagatgcc gatcggtacg ctaaagaaca
     6241 agaagcacaa gctcaaaaag tcaaagaagt gactgaagcc gaagcagaac gattccgtgt
     6301 ggaagcactg gcagaagcgg aagccaataa gacacgttta gctggacaag cagaagccga
     6361 agcagcacta gcaaaaggaa aagcagaagc cgaagcaaaa caaaagatcg ccaatgcctt
     6421 caaggaatat ggcgaagcag ctgtcttgag tatggttatc gatatgctac cgcaattgat
     6481 gcgcgaagca gcacagccgc taggaaatat cgaaaaaatc tctgttgtcg atacaggaag
     6541 cagttcagga gaaaccagtg gagcgaaccg agtgacaaat tatgccacaa atttgctttc
     6601 ttctactcaa gaaacactaa aagcgacaac aggacttgac ttgaaggaaa tcgtagagaa
     6661 tttgtcgaca cgtgggacta ctagccaagt aaatatgtat gaagcgccaa aagaggcaga
     6721 acaaaactcg atgaaggaag aataaagtag tacacttcac cgttcaatca aaaatggagg
     6781 aagacttttt aaagttcttc tctccatttt tttattttat tgcaaaagaa aattagtcct
     6841 cgctattttt cttttgtttt tggatcttag cttctatctc ttgtaaattt gttcttagca
     6901 atcgttcttt cttttggagt tcgaccaaat cttctgatag ttcttccttc ttttctgttt
     6961 caggaggacg aagaaagaaa ttgataccga ttgcaatgaa tgtccctata aaagtatcta
     7021 gtacacgttg gatagcatac aaagcggatt cgccttgagg gatgctcaac gcaatcagca
     7081 acaacgtagc tattgcggaa atgataccgg aattattgtt aataccatct gacacgacga
     7141 tcacaataat aactagaata ggaagcaaga tcaattctac caaaaaatca ttgtgaaaat
     7201 agcttttgac aaaaaaataa atgatagcta atgtaccacc taatgtattg ccgatgatcc
     7261 tagactttcc aaaagaaacg gttgtagtca agtcctgacg caaagaaaaa acagcagata
     7321 atgcagcaat catcgggatt cctcgttgag tcaaatgaaa aaacaagata cataaaaaaa
     7381 cagctaaggc acttttgatc gtacgcatac ctagtcgaaa acggccaaat tgcatagaga
     7441 ttctcctttt tggtcttttt actatcatac ctaaactttt gtctgatagc tagtttttca
     7501 ttattcaagt ataattaaaa taaaaaaggg ggaagtaaat gcgtatagga atgcgaacag
     7561 tcaaaacagt gatcagtgtc ttcttttcga tggtcacagc gggtagctta tcgctgttgt
     7621 attggccagc tgcagggatc attgcattat taagtgtagg aaataccaag cgctctacat
     7681 tgcgtacggg catgaatcga atcgcagcct ttatattagc tacggcaaca gcactgttca
     7741 gtttttttgc tgttgggtat actatttttg gcttcagtct ttttttattc ctgttcatcc
     7801 cgcttgcctc tcgcttcaaa ttaaccgaag ggatagtagt aaattcggtt ttggtcactc
     7861 attatttagt ggaggaaaac atgtcttggc agctgatcgg caatgaagcg ggattaatgg
     7921 cgattggatt agtatttgct ttgcttgcaa atgtttacat gccagatcgt acgaagcagc
     7981 tgaaggaaaa ccaaatacag atcgaaaaag aatttcgaaa tattttgaga caaatggcag
     8041 aatttctttt atcagaaaat aaagaagatg tgcagatcaa atgcgaacat ttactgacat
     8101 tcattcgcga aagccaagaa gatgccagag aacatcaaga aaactactgg ctcagacagc
     8161 cgctatacta cgaaacttac ttctctatga gaagagcaca ggccaatgtg attaaagaca
     8221 tgctggaaaa tctagagaga atccagcaac cagcttatta tggaaaacat atctatggtt
     8281 tattgattta tactgcagaa acattttcag agtcaaatga tggcagacaa attttaacac
     8341 gtattgaaga agtttatgta ttgtatcggc aaatgcctct gccgactagt cgccctgaat
     8401 tcgaggatcg agcagaattg ttccagtttt tgcagtcttt taaaagtttt attgaaatca
     8461 aggcagaatt ctcccaacaa aaaatccaaa aatagaggtt gtgaattcag ctaatattca
     8521 ggtcaaaaag ctgatttcac aacctcttct ttaaacggtg ttctcctgtc aactcctcgg
     8581 tcacaagtca caatattcaa caaacatgag aagctgtttt ttgaaattgc tccttgtgac
     8641 tcggagctga acggtaggct cacaacctct ttcttaaacg gtgttcaaaa gctttcctct
     8701 gcggtaataa gtccagccaa acgaaaaata ggaggagcta tttttgtttg gctgttctta
     8761 ttacttgatg cagaacagct ttttcacaac cttttaaaat tacatatttt tccatagaac
     8821 ttgcatttga tccaagattt cgatgacatc ttttgtcaag aagagagagc gattgggcaa
     8881 gtcaccttca atcatgtgta ccatattctt tatttcgtaa ttcattgctt cagatgtatt
     8941 cccgctttcg atgaattcct ttgtaccatc tgtaaataag atctccgcac gatcagcacg
     9001 cgggtaatca gcaatcgtca cataaccgtt ctcaaaggca acgataccga cttttggcat
     9061 ttttgcctga aaagtcaagc tgacagcagc aagttcattt tctttgttac gcaagatagt
     9121 gacggattgt tcgtccacgc ctgtttcaaa aggaaccatt gtagaggcaa ctacttcagg
     9181 ttgagagctc aaaaagaaac gagcgaaaga tacagcatac gtaccgatat ccaacagagc
     9241 tcctccagct aattctggat tgaagaaccg gtttttaggg tctggttctt tgtaactgcc
     9301 gaaaggtgct tggatcattt ttaaagcccc cagttttcct gtatccatta ttgagcgtaa
     9361 ctgctgataa agtggcatgt tgaagattgt catcgcttct gctaagatca aattcttttc
     9421 ttctgctaag cgcatagcgt cagccagttc ttttttattc atcgtgatgg ctttttcaca
     9481 tagaacgtgt ttgcctgctt ccaatgctgc caagatatgg tcgatatgct gtctatttgg
     9541 tacagcaatg tacacgatgt cgatttcagc atcgtctaac atttcttgat agcttccgta
     9601 agcttttgga atattgtaac ggtgagcgaa gttttccgct ttttctgaag ttctggaagc
     9661 aactgctgtt agttcgctcg tctcttgatt gaagctttcg gcaaactcat gtgcgatggt
     9721 accaagacca acgattcccc attgatattt tttcatctga aaacgctcct tcatagttgg
     9781 attgagtttc ttgtctgtct attattataa caaaaatcac gaagaataaa gaggagagga
     9841 cttgaaacat taaaaaaaat gaaagcagcg atttaagagg tgctttttga tcaaaactta
     9901 agtagaatag ttaaaaggag gggataaacg tgcatcaagt atatattgat cttattatga
     9961 gtgtattatt agaacagttt gaaactgaaa aaaatttttt agatgaatac ttgctgatca
    10021 gtccagaaca atggcaaaaa tggaaagatg gcgagaaaaa tctgccatct gaagcgatgc
    10081 agaaagtaaa aaatcttttc actgattatg aatggatgct aacgcaaaag attttgcgtc
    10141 aaacgattat ttttcccgaa aaacgaaacg ttgccgttgc agagtacaag caaatcaaaa
    10201 cacgcatcgc gcaaaaatgg cttagcagca aagcagcaaa agtcgagctg atccctttga
    10261 aaaataatca gcaggctggt agataccttg acttgaaagt aagtattcaa tatgatgaat
    10321 ggggctatga tgatattttg aattttcgtc tgccagcaga tgttcaaaaa cagatcgaag
    10381 gagaaaaaat cgaacttctc gattgggtga atgaaaatct ggaagaaact tacgttcatg
    10441 aggcgaatgg ggaacgtgaa gaacaacgaa aggatttttc aagtgaataa atttttagga
    10501 tttggagcag ttgtgattat gatcgcagca atgggctatg ttatttttag cgatgatgat
    10561 tcgcatgttc aagccggcaa tcaagcagtc gaacgaacaa gcaccaaaac aactgctagt
    10621 aaagaaaaga acagtcaaac aaccagcagc aaggtaaagg acaaagaaac aaaagatcgt
    10681 acgtccaaac aattgccaga tgttaaaaca gacgattgga agctagtttt ggttggtcca
    10741 gatcacaaat tagaaaaaga aattgatgag tctactcaat tggcatcatt aagtaacgga
    10801 tacttgatcg acaaacgcat tgctccagaa tatgaagcat ttgccaaggc tgctgaagca
    10861 gctggatttc cattagtgat ggtctcagct taccggtctg tatcatccca acaacaagtt
    10921 ttcgaacaaa atgtccaaga tgtgatgagt cgtcaaggtg tatcagaaga ggaggcgaca
    10981 aagattacta aagaaacgat tacggagcca ggatacagcg aacaccacac aggtctggca
    11041 gtcgatgtcg tcgatcaaaa ctggtacaac agttatcctt ctactgtatt ggatgcaagt
    11101 tacggagatc agcctggagc taaatggatc gcagataatg ctgcgcagta tggatttatc
    11161 gttcgttatc caaaaggaca cgaagatata actaagatca catatgaacc ttggcactta
    11221 cgttatgtcg gtaaagaaaa cgctgagtat atcaccaaac accaattgac gctagaagaa
    11281 tatctgaatg aattaaatga aaagtaggaa ttgatcatgg cgaaaaaaaa gtggaaaaag
    11341 caaaagcgca agaatatccg ttggccagca gtggttgccg gtgtagcagt gatactctta
    11401 gcctttgttt tttccttgaa gaatctgtct tctcctttta ctgataatca gcaagaaaat
    11461 gaagcgcaat cccatcagca atttattgaa cggcttgttc cgcatgccca agaactacaa
    11521 gatggttatg gagtgcttcc tagtatcatt ttaggacaag ctatattaga atcaaattgg
    11581 ggaaaaagta ccttggctag caaatacaat aatcttttcg ggatcaaggc ttatggggat
    11641 cagaagaaag taagcctgga aacaaaggaa tttgttaacg aagaatggat aactatccaa
    11701 ggagacttca aagtgtatga ttcatgggaa cagtccatgg atgaccatac acaactgttc
    11761 gtccaaggag tagattggaa tcctgcgctg tatgaaaaag tgattacggc caccaactat
    11821 caggaagccg cacaagcatt gcaagatgca ggttatgcga cagaccccgg ttatgcgcaa
    11881 aaaatcatcc aggtcatcga gacgtatcaa ttgaatcagt atgaccggag atagcaaagg
    11941 agaagtggct gatggcagaa caatttacgc cagaaatcat gacggaattg agaaaagata
    12001 tcgtaagagt accggaaatc attttagaag caagcgggat caagatattt ggaaaattga
    12061 tccgttcgat catctttact acggatattg cgatcatacg taatacaaat gcgaatgcag
    12121 taattgctgt gtatcctttt acacctcatc cagcgattac taaaagcatt attgaggcag
    12181 ctgacattcc agttttctcc ggtgtaggtg gtggtttgac acaaggtgct cgttcttcct
    12241 acatgagcat gttcgcagaa gcgcaaggtt cgttaggggt cgttttgaac ggtcctacgc
    12301 ctttagagac agtaaaagaa gtttgtcgag tagttgatat tccagttatc agtacggtaa
    12361 cgtcgaaata tacagaaata gatgaaaagt tagcagctgg tatttcgatt atcaatatca
    12421 gtgcaggtaa agaaactgca gaagcagtac gccattttcg taaaaaatat cctgaacttc
    12481 ctatcattgc gacaggtggt ccaacggaag aaagtattgc tgagaccatt caagcaggag
    12541 caaatgccat cacatatacg ccaccaagta gcggagagct ttttagtcgt aaaatggaaa
    12601 aatatcggga aactgagaaa aagaatcatg aataacaggt gagaatcaaa agacgaggaa
    12661 gagcagatag tggctcttcc tcgtcttttg tactccagta ctatttgttc agaattcaaa
    12721 aaaaagagaa atttttttgt ttttttacct gtaatgtaca taagaagagt taaaaccttt
    12781 tgttattatt agcattctaa caaaaaaatg tcttgattta ttgaagcgct ttctatagaa
    12841 tgaaaaacga ataaaaacta aaaaaggcac ccgacaattt agtagcagct aaattgtcgg
    12901 ggcttgaaaa gtcagcggga actgaccaat caagttgcca tagtcaatgc ttgtgcatcg
    12961 acgtgtatat tgtacctaat ttcatacaga attactagtt atttttctgt tttttaggga
    13021 agagcgcaca cgaagaagca tgcttattta gctatgtcaa ggatattggg cagttgtctg
    13081 atatcctttt ttctttgcgt aattatcctc ccatcattga agcaagaagt gtttttagcg
    13141 atgaaagaag aatcagcact atctaatttt tcttgaaagt attgagttgt tcatcctagg
    13201 cagaacgata aggcaagtct tggtttttat tccccttgaa aaagatcgtt taataaatat
    13261 gttctgtata aaaatacagt atattgaaaa ataacaatgg aagtagaggt gcagagaatg
    13321 aagaaaagaa tgagaatcat cttagcttta agtgttatgt tattcttctt gacggcttgt
    13381 gggcaagaag atgacaaaaa aatcacgctg aacatggctt ggtggggttc gcaagtccga
    13441 cacgatgcaa cagtaaaagt aatcgagctt tatgaagaaa aaaatcccca tgtgacgatc
    13501 gattatgaat tttatgatta tgaaggatat tacacaaaat taggtacact agcagcatca
    13561 gatgatatct gggacatttt ccaactgaac aatcaatttc ctcaatacat caatcagatt
    13621 gaaccattag atgaatatgt ggagcaaggg ataattgata tttctaatgc agatgaatcc
    13681 tatttagcaa cgacgactta cgacgggcaa ttagttggac tgtctaacgg ggtgaattcg
    13741 tttgcgattg cttacaataa aaatatattt gaacaattaa atatccctga accagcttca
    13801 aattggacat gggatgaatt tgaacaaatc agctatcaaa tcactgaaga attaggaatc
    13861 tatgctattt caagattcga agattttata gctggctgta ttattcaaat accacaagtt
    13921 gagaaaggat tgaattttta taatcgggaa gatctatcta acagcttggg atttgaaaat
    13981 ccagactatc taacagattt tttccagctg cgtaaaaatc tggtagacaa aggggcccat
    14041 ccagaaccag gtggtggagc tgcaagtacg acggatctgt cttttcaggc ggttagggat
    14101 agagaagcgg caatggtgtt tctatcaagt aaccagttga cagaaatatt gtcaggggct
    14161 cctgaagata tggagattca gttgattaat ccacctcgca gaaaagcaga tggagaatct
    14221 ggtatccctt tacgttcttc gcaaatgctg agtatggcaa aaagctcagc ccataaagaa
    14281 gaagctgcta agtttattga tttctttcaa aacagtgaag aagccaatga aattttaaaa
    14341 ggagaacgtg gtgtaccaat catgtcgaat attcgagaac agctcgctcg tgcagatgat
    14401 gcaactttgc aagcgacatt tgattattta gaaatgattt ccgatatgga caacggcgaa
    14461 gtgaatgtct ttgatagtgc tttgaatccg gaaattcaag accaatacaa tcttttagtt
    14521 gaaaaagtca tttatgaaga agtaacagca gaaggaaggc atgaaaaaag aaaaactaaa
    14581 acggttcttg gatcaagaca atgtggtcgg ttacgtattt gcttctccat tcatcctagg
    14641 atttctattg ataacagtga taccgatgat cttgtcactt tattattcgt tcaccgatta
    14701 tcgtttggga aatccagtct catggatcgg cttggataat tatataaaga tgctgagtga
    14761 tgcccgtttt ttgaactcca tccgagtcac ttttctctat gtatttgcat ctgtacctgt
    14821 gaaactgatc tttggtctat tgattgctta tcttttaacg aggaatatca aaggaacgac
    14881 tttctttcgt tctttgtatt atgtgcctag tttgatcggt ggcagtattt ctatcgctct
    14941 agtttggcgg gagttgtttt ctaaaaacgg gttagtgaat attttcctgg aacgattagg
    15001 cgcacctgcg ctttcctggt ttagcgatca aaaactggtc atgatccctc ttgttttgat
    15061 gtctgcttgg caattcggtt cggcaatgat catctttgct gcaggtatca aagagatccc
    15121 aggctcgtat tatgaagcgg cagagatcga tggagcgaac aaatggcaga cgttttacaa
    15181 gatcacgtta cctcttcttt cgccgatcat tttgtataac ttggttatgc agacaatcgc
    15241 ggcgtttatg acttttacgc aggcatttat cattacccaa ggtggtccga atgatgccac
    15301 gaacttttat gctctttatg tgtataacca agctttttct tttaattcta tggggtatgc
    15361 tagtgctatt tcgtggatca tgctgttgat tatgggagct gtcacctttc tgattttcaa
    15421 atcatcaaaa aaatgggtcg ttgtgtgaat gagaggagga agaaatgaat aatacagtaa
    15481 cgaaatggtt tcaatacggt tttattttgc tattaggtct agtcatgatt tatccggtct
    15541 tatggatgat cgcaggttct tttaaaacgg atcaagagat tatgagtggt tcgctgaatt
    15601 tgattccaga gacttttcaa tgggaaaatt acgcaaaagg atgggcgggg tttgctggaa
    15661 tttcattttt tactttcttt aggaattcgt ttgtcatctc tgcagtttcg acgataggga
    15721 cagttctgag ctcgacttgt attgcttacg cattatcgag aatcgatttc aaaggaaaaa
    15781 gattctggtt cgtcgtcatg ttagtaacga tgatgattcc tgcacaagtg attttgattc
    15841 cgcagtttat tatttacaat cggctgaatt tggttgggac gtatgtgccg ctgattttac
    15901 cacatttttt tggacaagca tttttcattt accagatcat gcaattcatg gtcagcatcc
    15961 ccaaagagtt agatgaatca gcgattattg acggatgcag caagtatagt gtctttacca
    16021 aaattatttt tcctttgctg aaaccttcga tcatcacgac gatcatcatc cagttttatt
    16081 ggaaatggga cgacttcatg ggaccattga tttatttaaa caagccgaga tcttacacgg
    16141 tatcgattgc catcaaatta tttgcagacg ctggatctag tacgacttac gcgagtatgt
    16201 ttgcgatgtc tacgttgtcg ctgctaccgg tttttctgat tttcttattc ttcaataaat
    16261 atcttgtcca ggggatcagt acaagcggtc tgaagggctg aatatgaaaa agccaataga
    16321 agagatgttt ctttacaagc tcaactacta tgtctgtcgg atttttttaa ccagtcttat
    16381 cagtaatgct cttttcgctt tagttctagg atgtttgttc actttaggat tagtgctgtc
    16441 tgctcgtgtt gtctatctga tttatttttt gatgatgtat ccttcttatt atctatctgt
    16501 ccattacttg gcagaaaaaa aggtatgttg gaggaaaggg aaaatcaaac ggatgtgttt
    16561 catcattacg ttgtttcagc tgtttgtcat tatttttgca gttgattggt atttattagt
    16621 gggaaagatt ggagcttggt atctgattcc ttttctattg tgtctgttgc tttttgtatc
    16681 ccatagtttt tttagtacgc taattgatat ctgcaaagaa gataaagcag atagtcttgt
    16741 ttatttgatc aaaaggaata tctggctgtt gtcaaaaaac gtcaaggggt ttctgtggta
    16801 tttatgcctt ttgtatggac tgttctggtg tatacagcct gattttttac tcactgcatt
    16861 actgtatcca gggctgccat ttgtcacagt attttgctta aaagaaaagg agaatgactt
    16921 taaatgaaga aaaaatatgt acaagtcgga cttggtggga gagcgcgttt tttttatgag
    16981 gcaattgcct ctacgtatca tcaaacttgt gaactagtcg gtttttgtga tgtatctcaa
    17041 acaaggatgg attatgcaaa tcggatactt gtgaacaagt atggacagaa aaaagtaaag
    17101 acatgtatgc catcacaatt tgagcaacta ctggatgacg tacggccggc tgaagtcatt
    17161 attacgtcag tagatcgaac gcatcatgat tatatcattc gtgcaatgaa aaaaggatat
    17221 gatgttattt gcgaaaaacc tctgactatc gatgaggaaa aagctcaaga aataataacg
    17281 gtccaaaaac agacgggaag aagcctgcga gtcactttta attatcgtta tgcaccgtat
    17341 cacacgaaga taaaggaact gatagaaagt gatgtgattg gtgatgtgtt ttctatccac
    17401 ttcgaatggc tgttaaatac gcaacacggg gcagattatt tcagaagatg gcatcgaaat
    17461 aagcacaata gcggcgggct tcttgttcat aaatctaccc atcattttga tctagtgaat
    17521 ttttggttga acagccgtcc ccaaaaggta tttgcattcg gtggattaaa cttttatggg
    17581 atgcataatg cggagaaacg cggagtagaa atattttaca gtcgatgcta tggaagcgaa
    17641 ctagctaaag ctgatccatt tgctatagac ctgtcagcca atgaggaatt gcgagaaatg
    17701 tatttagaag cagaaaaaga cagtggatat attcgtgacc gcagtgtatt ttccgatgat
    17761 atctctattg aagatacact aggcgtaacg gtcaaatatg aaactggtgc gattttgacg
    17821 tattcgctaa acgcgtatat gccgtgggaa ggattcaatg tttctttcaa tggcagcaaa
    17881 ggacggatcg aaatgaaagt gatcgaaaag tcttacatca atgcaggtgg agataaaaag
    17941 gatgaaggag cagccgttct tcagtccatc catgtctatc cgatgttcgg tgagccatat
    18001 gaggtaatcg tcgaacaaga agcgggcgga catggcggag gagatccgcg tttattagat
    18061 gatctatttg gtgaagaaaa agaagaagat ccgtggaatc gggcagcaac tcacgtagat
    18121 ggtattttgt cgattttgac aggaatcgca gcaaatcatt ctattgcttc aggaaaagca
    18181 gtagtgattg acgagcttgt cagcttaaaa gaataagaat agatttacgg aaattacttc
    18241 ccgtagttta taaaatcttg ccttattgcc gataggctgt cttttgaaca ctatttattc
    18301 ggaaaaaggg gctatgacat gctttttgtc atgccttctt tttatgtggg ctgctttttt
    18361 agataggaga aaataaaaat aactatttaa acccgaacaa aaaggaataa tgcattaaaa
    18421 atcattagat tttacaaaaa aataagtaaa ccacgttgtc agtttcagaa agtagtgtta
    18481 gactgttttc aatatttgaa acaacatttt ttattcaaat atggcaaact gccttcacgt
    18541 gcaaaacagt tgcaagaaaa tgaatttaca ctattattag ttttacatac ctacttatat
    18601 atcgtcataa tatggatgac agtttctagc cagtaccgta aatgctggac tataagtaaa
    18661 agattggcta tttcaccctt cttttcttat gataaagtgg agggtgtttt tctttaacag
    18721 aaacatagta ggtttttgca caaaagcggt tatttcggta ttaataaatg ataaaggagc
    18781 aaataaagtg aaagaattag ttgaacgtat tcaaaaagac ggacgtatat taggcgaggg
    18841 cgtattaaaa gtcgacagct tcgtcactca ccaagtagac cctgtattga tggaacaaat
    18901 gggaaaacgg tttgcagaag tatttaaaga acaaggaatc acgaaagttg tgaccattga
    18961 agcttctggg atcgcaccag ctttatttgc tgcaagagaa ttagatgttc caatgatctt
    19021 cgctagaaaa gcaaaaagtt tgacgatgga tgaagaatta ttgacagctt ctgtttattc
    19081 ttttacgaaa caagtgacaa gtacgatttc tatttctcgt aaatttttat cttcagaaga
    19141 caaagtattg attatcgacg atttcttggc aaacggacaa gcggcaaaag gattgatcga
    19201 attatgccag caagctggag cgcaagtaga aggaatcggg atcgtcatcg aaaagtcttt
    19261 ccaagatgga cgtcagttac ttgaagaaat gggattgaga gtcgtttctc ttgcgcgtat
    19321 tgcttccttg acgaatggac aagttgagtt tcttgaggag gacgcataat cgtggaaaaa
    19381 gaaacacaaa atggaaaagc tgcggtttta gggctgcagc atctgctagc gatgtatgca
    19441 ggagcagtag cggtgcctct tttgatcggt acaggactag gctttaatca agaacagatg
    19501 acttatttga tttcaattga tatctttatg tgtggattag cgacattatt gcaattgaca
    19561 gttaaccgat ttttcggaat tggcttgcct gtcgttttag gatgtgcgat ccaagcagta
    19621 gctccactga tcttgatcgg aaccaatgaa ggagtaggag ctatctacgg atctattatc
    19681 gcttctggga tctttgttgt gttagtttct ggtgtctttt ctaaaattaa aaaactattc
    19741 cctcctatag taacaggaac ggttatcacc gttattggtt tgactttgat tcctgtagca
    19801 atcgagaaaa tgggtggagg taatgcaaca gcagctgatt ttggtgacaa aacaaatctg
    19861 ctacttgcat ttgtgacgat tctcctaatt gtaggcgtgc aaatgctggc aaaagggttc
    19921 attcgttcaa tcgcggtatt gattggttta gtcggaggaa gcatcttagc tgctttcatg
    19981 ggaatgattg atctgagcgc gattggagat gcgccgttgt tccatgttcc acaacccttt
    20041 tattttggta aaccaacttt cgacgtatgg tcgattttac taatgatcat catttccatg
    20101 gtcagcatgg tagaatcaac aggtgtatat tttgcacttg gtgacattac tgggaaaaat
    20161 gttggagaag aagaattgaa aaagggatat cgtgcagaag gacttgccgt tatcttaggc
    20221 ggtattttca atacattccc ttatactggc ttttctcaaa atgtcggatt ggttcaactt
    20281 tcgggaatca aaacaagaaa acccatctat ttctctgcat tcttcttgat tgttttagga
    20341 ttattcccta aaatcggtgc gttagcacaa atcatccctg agccggtact tggtggagga
    20401 atgttagtca tgttcgggat ggtggcagtc caaggaatcc gtatgctgtt ggaagttgat
    20461 ttcagcaatg ataaaaatct attgattgtt gctgtttctg ttggatttgg gctgggtttc
    20521 aatgttacgc cgacattatt cagtcaaatg ccgcaaacat tgcaaatgtt cactggaaat
    20581 ggcatcgtaa tgagtagcat tacggctatc atattgaatt tgatctttaa tggattgaaa
    20641 aaagatgaag aaatattgga agaagccttc aagaagtaat agagcaaaag aaaggttgtg
    20701 aaatcagcgt tttgacctga ttgttagaga aacaaacgga aaaatagttc ctcgtatttt
    20761 gccgtttgtt tgagctaata ccgagggtca gctgattgaa caccgtttat ccataaaaag
    20821 gaatgtgacc taacttttgt cacattcctt tttttcatct ttaataaaca cgaacaataa
    20881 aatgtttttc tttgttaaca ttcgaaattt ctttgacatc aaaaaaagtg attgttaaaa
    20941 tatgaatgta gaaaaactgg gaggaaaaac aatgacagca aaaatctctg tgattatggg
    21001 gagtacttca gattgggaaa caatgaaaga agcatgtcaa atattggatg aattcggtgt
    21061 gtcttatgaa aaaaaggtcg tttctgctca tcgaacccca gatttgatgt ttgaatatgc
    21121 acaaaacgct cgaaaaaaag gaatcaaaat cattattgca ggagcagggg gagccgcgca
    21181 tctgccgggt atggtcgcat caaaaacaac tttgccggtt atcggtgtac cggtccaatc
    21241 acgaacatta aatggtttgg attcgcttct ttcgattgtc caaatgcctg gaggagtacc
    21301 tgtagctact acagcaatcg gaaaagcggg agcaattaat gctggactac ttgcgataca
    21361 gatgctttcc atgtatgatt tagaattgga ggctaaattg gctgaaagac gttcaatgtt
    21421 ggctaaaact gttatagaaa gtagtgatca acttggttaa acctttgttg cctggttcaa
    21481 cgatcgggat cgtcggtggc ggtcaattag gtcgtatgat ggcgattagt gcaaaagaaa
    21541 tgggctttaa agtaggtgtg ctggatcctg tgagcgattg tccagccgca caagttgcag
    21601 attggcacat tgaaagtgtc tatgatgaca cctttgcgct gaaagagctt gccagaagaa
    21661 cagatgtcat tacctatgaa ttcgaaaacg tcagtgtaga ggcgttaaat gcgattttac
    21721 ccatgtcatt tattcctcaa gggacagact tgcttgcgat tacgcaagac cgcctgttag
    21781 aaaaatcttt tttggaaacg aataacatcg tgattgctcc ttatgcaacg atcgtcagtc
    21841 cgacggatat ccaagatgcg attgacggga tcggctatcc ttgtgttttg aaaaccactc
    21901 gaggcggcta tgatggcaag gggcagtatg ttttgaaaag tcgggcggat ctagctcctg
    21961 ccatggacct tttaagagaa ggaacctgtg ttttagaagc atggatcccg tttgaaaaag
    22021 agatttcgat catggtggca ggaaatggtc aaggagattt cacgactttt ccggtcgtgg
    22081 aaaacatcca ccacaacaat attcttcatg aaacgattgc tccagccgcg attgatcagg
    22141 atgtgatcga ggaggctgag cgtattgccc gtgtgattgc agaagcagtg agcctttcag
    22201 gtgttttagg ggtagaaatg tttttaacaa aaacaggcgg gctatatgtg aatgaattag
    22261 ctcctcgtcc tcacaattct ggacattatt ctattgaagc ttgttctatg agtcagttcg
    22321 atgcccatat tcgaggtatt tgtggttggc cattaggaga aaaagtacgt ttgataagca
    22381 atgcagtaat ggtcaatgtt ttgggagaac aactgctgga aagctatcgc ttgattgaga
    22441 ataagccaga ctggcaattc catttctatg gaaaaaaaca agcaaaaaaa ggtcgcaaga
    22501 tgggacatat cactatcttg acagaagaca tccagcaaac gctggaagaa atcaaacaaa
    22561 cgaatatttg ggattagagg agaaaaaaca tgttagaacg ttatacacgc cctgaaatgg
    22621 ggaatatctg gacggaagag aacaaatacc gtgcgtggct cgaagtcgag attttagcag
    22681 atgaagcttg ggcagaactt ggtgaaatcc cgcaagaaga tgtgaaaaaa attcgtgagc
    22741 atgcatcatt tgatatcgat cggatcttgg aaatcgaaaa attaacacgc catgatgtcg
    22801 ttgcgtttac acgagcggtt tctgaaacat tgggagaaga aagaaaatgg gtccattatg
    22861 gtctaacaag tacggatgtc gtagatactg cttatggcta tctcatcaag caagcaaatg
    22921 atattttacg ggaagacttg aaacgtttta cggaaatcat tggcgaaaaa gcaaaagaac
    22981 acaaatatac agtgatgatg ggtcggacac acggtgtcca tgctgaacca actacttttg
    23041 gattgaaact tgccacatgg tattcagaaa tgaaacggaa catcgaacga tttgaacatg
    23101 cggcaaaagg agtagaagca ggaaaaatct caggagcagt gggaacgttc gcaaatatcc
    23161 cgccatttat tgaagagtat gtatgtaaac aattggggat ccgtccacaa gaaatctcta
    23221 ctcaagttct ccctcgggat ctacatgctg aatatttctc agcgatggcg cttattgcta
    23281 caagtattga acgttttgca acagagatac gcggattgca aaaatccgaa acacgtgaag
    23341 tagaagaatt ttttgcgaaa ggtcaaaaag gttcttctgc tatgccgcac aaaaggaatc
    23401 cgatcggttc agaaaatatg gctggtctag ccagagtggt gcgcggacac atggtcactg
    23461 cctatgaaaa tgtcacatta tggcatgaaa gagatatttc tcattcttca gcagaacgaa
    23521 tcatcttgcc tgataccacg attttattga attacatgtt aaatcgtttt ggcaatatcg
    23581 ttaaaaatct aacggtcttt cctgaaaaca tgaagcggaa tatgtctgca acatttggtt
    23641 tgatttatag ccagcgcgtt atgctgaaat tgatcgataa gggaatgaca agagaagaag
    23701 cttatgatct tgttcagcca aaaactgcgt acgcttggga caaccaagtg cagttccgcc
    23761 cgctgttaga agaagatcca accatcacat cggtattatc taaagaagaa atagacgatg
    23821 cctttgatta tcatcatcat ttgaaaaacg ttgacgtgat attcaaacga gtaggtttgg
    23881 aataaagaag agtgagttgc aaaattcaat taaaatagaa tagcaaaaat aaccaaaaaa
    23941 gaagcagatc cgttagcggt tctgcttctt ttagtttatt atattttctt atcaataata
    24001 ggaacctgat tttaaaagcg gctacctctt taccgtgcct ctttacatac aaaaaataga
    24061 ggaattaagc gtaaatggct tggctattat aactaaaatg ctggttatgg agatgttgca
    24121 gcaagttttc gagaaaagcg tattccatct cttgtccatt gaataagatc tggatacgat
    24181 aatgattttt gaaacgcttc tgttcatgaa tctgactgat tgaaagttcg cctttgtgtt
    24241 ctgtctgata aagaaaagtc cttttacggc attcagcaca gcgcttggtt gctcggcgga
    24301 tttttcggaa tgtataaaaa ctagtatctt tattgcataa ataaacgtct ttgatcgaca
    24361 gcaaatcatt atatagacag gaacgttctt cagctgttaa ttctgataaa aaaagctgac
    24421 cttgttttat tgcatcttcc atcggcaagt gcctcctcga acccattatc caaattttta
    24481 gttaattcta atataccatc attaagtata aatgtaaacg cttgttgatg gtataagatt
    24541 ttcgtttctg ataaaacacg aacattaaaa gaatctaatg ccttaaatgt acatattttt
    24601 gttgatttat aaaaagatga ttgctaaaat ttaaagtgaa aaaacgaaca aaaaacgatg
    24661 tatttgcttt taatgtaaat aaaaaggaga gacgtacatg gagaagaaag aacttttgta
    24721 tgaaggaaaa gccaaaaaaa tatatcttac ttctgatgag aacttgattt ggatagaata
    24781 tttagatcaa gcaactgcgt tgaatggcgc gaggaaagac cagataagtg gaaaaggaga
    24841 attgaataat caaatcacaa gtgcaatttt ccgttatctg actcaaagag gaataaaaaa
    24901 tcattttatt gaacaattgt ctaaaaccga acaattagca aaaaaagtac aaattatccc
    24961 tctggaagtg gttatccgaa atattgcagc aggaagtttc tcaaaacgtc taggtgttcc
    25021 cgaaggtaaa gaattcatca aaccaatcat tgaattctac tataaggatg accagttgga
    25081 tgatccattt atcaatgaag agcatatcta tttcttacaa cttgcaaaac cggaagaaat
    25141 cagtgagctg aaagcccaag cgttggctat taatgaaatt ttgaccgaat tatttgctaa
    25201 ggtcggtttg gttttagtag atttcaagct agaatttggc cggtcgaatg gacaaatttt
    25261 gcttgcggat gaagtaacac cggatacttg ccgattatgg gatgaacgta cgaatgaaca
    25321 tatggacaaa gacgtttacc gcagagatct aggtgatttg atcccagtct atcaagaagt
    25381 atataaccgt ctatcggaac taactgaaga aaaggagcga gtatgatgta tttcgtaaaa
    25441 gtatatgtaa cctataaaga ttcagtgtta gatcctcaag gggaggcagt aaaaaaagca
    25501 gtccatcgta tgggatatga aacaatcgaa gatatccgaa taggaaaata ctttgagata
    25561 caacttgctg atgcagaaga accaatcgat acagtgattg aaacgatttg tgacaagctg
    25621 cttgcaaatg taaatatgga aacataccga tatgaaatca aaaaaatgga ggaagtataa
    25681 catgaaattt gcggtcatcg tttttccagg ttcaaattgt gacgcagata tgttatgggc
    25741 agttcgcgag atcatgggcg ctgatgccga atttgtacgg catgatgagt cctctttaga
    25801 aggatttgat ggggttttac tgccgggagg attctcctat ggtgactatt tgagatgcgg
    25861 tgcgattgct cggttttcta caatcatgga agaagtgatt cgttttgctg aagaagggaa
    25921 accagtattc ggtacatgca atggattcca gatattgaca gaagcaggac ttttgccagg
    25981 tgtactgaga cgaaatgagt ctttgcattt tatttgcaaa actgtaccat tgaaagtcaa
    26041 ccccaagacg gcctttactt cagagtatga agccgacgaa ataatctatc ttccagttgc
    26101 tcatggggaa ggaaattatt tctgtgacga agaaacactt caagaactaa aaaaaaataa
    26161 tcagattgcc tttacttata atgacaacat caacggcagc gtggaaaaca ttgcagggat
    26221 tacgaacaaa gcagggaatg tgttaggaat gatgcctcat ccagaacgtg cagtagaaga
    26281 cttgctagga tcagaagatg ggaaacggtt ctttgcttcc attttaaaaa acttcggaaa
    26341 ggtgaccgta tagatgaaaa cattgaaaga accgacagct gaagagatca agaaaaaaag
    26401 aatctatgcc gaatggggat tgactgatga agaatatcga atgatcgaag aagatatctt
    26461 aggaagaatg cctaattata cggaaactgg actgttttct gttatgtgga gcgaacattg
    26521 ttcctataaa aattctaaac cggttctacg caaatttcct actagtggac ctcatgtgtt
    26581 gcaaggccct ggagaaggcg caggcatcgt cgacatcggt gatggactag cagttgtctt
    26641 caaagcagaa agtcacaatc atccttcagc cgtggagcct tatgaaggag ctgcgacagg
    26701 agttggcgga atcatcagag atattttcag catgggggct cgtccaattg cgttgctaga
    26761 ttcattgagg ttcggcgaat tagataatcc aagaacgaaa tatttactag aagaagtagt
    26821 tgcaggtatc ggcggatacg gcaattgtat cggcataccg actgtaggcg gcgaaattgc
    26881 atttgatcct tgttatgaag gaaacccatt agtcaatgca atgtgcgtag gtttgattga
    26941 acacaaacat atccagaaag ggcaagccgc tggcgtcggt aattcgatca tgtatgtagg
    27001 tgcaaaaaca ggtcgcgacg gtatccacgg agcaactttt gcctcagaag aatttaatca
    27061 agaagaagaa cagcaacgtt ctgctgtaca agtaggcgat ccttttatgg aaaagctgct
    27121 gcttgaagcg tgtttggaat taatcttgca tcattcggat tctctaatag gtatacagga
    27181 tatgggagca gcaggacttg tttcatctag tgcggaaatg gcttcaaaag ctggctcagg
    27241 acttatattg acattagatg aagtcccaca aagagaaact gggatgacac cttatgaaat
    27301 gatgttatcg gaatcacaag aacggatgtt gatttgtgtt aaatcaggag aggaagaaaa
    27361 aatccaagaa ctatttcaga agtatgatct agatgcagtc actatcggta aagtaacaga
    27421 cgacgggcaa tatcggctat atcatcaagg agaagaagta gcaaatctgc cagtcgatgc
    27481 attagctgaa gatgcaccag tttatcataa agaaatgaaa gaaccagctc gaatcgtaga
    27541 atttcagcag ttagctcctt atcagccagt tatcgaagaa cctggacaag tattgcttga
    27601 tctgctgcag caaccgacga ttgcttcaaa acgttcaatc tacgaaactt atgattcaca
    27661 agtacaaacc aatacagttg tccgtccagg aagcgatgct gctgtgatga gggttagagg
    27721 gacgaataaa gcgttagcta tgaccacaga ctgcaacgcg cgctatctgt atttggatcc
    27781 aaaaatcgga ggacaaatag cagtggcaga agctgctaga aatattattg ctagcggtgg
    27841 taagcccttg gcgatcaccg actgcctgaa ctatggttct cctgataaac cagaagtttt
    27901 ttgggaatta tcgacttcag ctgacggaat tgctgctgcg tgtgaaacgt taggaactcc
    27961 tgttatttca ggaaatgtct ctttatataa tgaaacggat ggtcaagcaa tctatccaac
    28021 accaatgatt ggtatggtcg gattgattga ggatcataaa catatcacta ctcaggaatt
    28081 caaaaaatca ggggatctaa tctatatatt aggtaagacg tttgcagatt ttgatggcag
    28141 tgaattacaa aagatgcagc ttggaagaat cgaaggtgtg atccgaaact ttgatctatc
    28201 cattgaaaag agaaaccaag agttagtatt gacagccatc caaaacggat tgattgaaag
    28261 tgctcatgac tgttcagaag gtggcttggc aattgcactg gcagaatcag ctttcaaaca
    28321 tcagctaggg atatctgtac agtttgaatt gtcctctgct caattatttg cagaaactca
    28381 atcaagattt gtgttgacag tcgcaccgga aaataagaca cgattcgaag aaatgatggg
    28441 agatgctgct gttttagcag gtaaggtgac ggatgaagcg ataatcgaaa tttcagcaac
    28501 tgacggtcag atcaaaatag aaactgcagt agcaagaaag tgctgggagg atgcaatcgt
    28561 atgtcttatg aagtgaagag tttaaatgaa gaatgcggga tcttcggtgt ttggggtcat
    28621 ccagacgccg ctagtgtggc atacttcggg ctccatagtc ttcagcatcg aggacaagaa
    28681 ggtgcaggta tcgtgtccaa tgatcgagga aagcttaagg ggcatcgaga tttggggctg
    28741 ctttctgagg tattcaaaga tcaaagaaaa ttgcagcagt taacaggaga agctgcaatc
    28801 ggacatgtac gttatgcaac agcaggcaat ggaagtgtgg acaatattca gccttttcta
    28861 ttcaaatttc atgatcagtc gatcggctta gcgcataatg gtaacttgat caatgctcgt
    28921 tcccttagaa aagagttaga ggaaaatggg gcgatcttcc attctaactc cgatacggag
    28981 atattgatgc atttgatccg acgaagtgaa gaaacagatt ttttaaataa attaaaaagt
    29041 gctttgaatg aagtcaaagg tggctttgcc tatttattaa tgacagaaac ggcaatgatt
    29101 gccgctttgg acccaaatgg ttttcgtcca ttagcaatcg gacaaatgaa aaacggagca
    29161 tacgtgattg cctcggaaac ttgtgcatta gaagttattg gggcacgttt tataagagat
    29221 gtccagcctg gtgagatagt gatcatcaat gacgaaggaa tccagattga ttgctttacg
    29281 aaggacacgc agctttctat ttgttcgatg gaatatatct attttgccag accagattca
    29341 aacatcgcag ggatcaatgt gcatactgca cggaaaaata tgggacggaa tttagcgaaa
    29401 gaatcgccag taaaagcaga tatggtcgtt ggcgtaccta attcttcttt gtcagccgca
    29461 agcggttatg cggaagctag tggaatccct tatgaaatcg gattagtaaa aaatcaatac
    29521 attgcccgaa catttatcca gccaacgcct gagctaagag agcaaggtgt tcgaatgaaa
    29581 ttatctgctg tacgaggagt agtggaaggt aaaaaggtga ttatggtcga tgattcaatt
    29641 gttcggggta cgacaagcag acggatcgtc catcttttga aagaagcagg ggcaaaagaa
    29701 gtccatgtga ggattgcttc tcctcctttg aaatatccat gtttttacgg gattgatatt
    29761 caaacgcgta aagaactcat tgcggcaaat cacacgattg atgaaatcaa agaatgtatc
    29821 ggtgcagatt ctttaggttt tttaagtgaa gagggtctga tcgaatcgat cggtctggaa
    29881 aaagatgctc cgtattcagg attgtgtatg gcttatttca acggagatta tccgacgccg
    29941 ttatatgact atgaagaaaa atatcaggaa tcactaaaag aaaaagtatc gttcttttaa
    30001 aagcagacca agaagggagt atatgaattc aatggcaaat gcatacgcaa aagctggcgt
    30061 agatgtcgaa gctggatatg aagtagtaga acggatcaag aagcatgtca aaaaaacgga
    30121 acgtttaggt atgatgggcg ctataggagg attcggcggt tgttttgatt tatcggtctt
    30181 tcaattaaaa gagccagtcc ttgtctcggg gaccgatggc gtggggacga aattagcagt
    30241 ggcaatcgaa gcagacaaac acgatacaat cgggatcgac tgcgtagcga tgtgtgtcaa
    30301 tgatatctta gcacaaggcg cagagccgtt atatttctta gactacttag caacggggaa
    30361 aaatgatccg ttacgtttgg aaaatgtagt aaaaggtgta gcagatggat gtgttcaagg
    30421 aaatatggcg ctgattggcg gagaaacagc tgaaatgcct ggtatgtact caggtaatga
    30481 ttatgatcta gccggatttg ctgtagggat agctgaaaaa tctcaattat tgacaggaga
    30541 aacgatcaac ccaggagatg tgttgcttgg aatagcatct agcgggattc attcaaatgg
    30601 gtactctctt gttcgcaaaa ttttctttga gcagcagcag ttttctttat catcaaaact
    30661 gccagaattg aaaaaaacac ttggcgaaga attgcttgag ccaacgaaaa tctatgtttc
    30721 ttccttgctt ccgctgatca aagaaaagaa aataaaagga gctgcacata tcactggtgg
    30781 agggtttgta gaaaatatcc cgcgaatgtt accagaatct atggctgcac aaatcaaact
    30841 tggctcatgg ccgattcttc caatattcgg ctgtttacaa aaatatggtg aaattcctga
    30901 gatggagatg tatgaaatct tcaatatggg catcggtatg gtcctggcag tcgaagaatc
    30961 gaaagtaaaa gaaatagtac acgaaattga gcaagctggc gaaaaagttt atgaaatcgg
    31021 gaaagtgatc gccaaagaaa aacatcgtgt ggtgtttata ggagaagaac gatgagaatc
    31081 gctgtttttg cttctggaaa cggaagcaac tttcaagcgc tagccgatta tctatcgaaa
    31141 aaaggattgg aatcttcgat tgactggttg ttttgtgatc agcctgaagc atatgttttg
    31201 aaacgtgcaa ctgcgttgag tgtaccggca gattgttttt cacctaaaga atttgattca
    31261 aaaaaagagt atgaagaagc aattttgcat aaattgaaag aaaaaaagat cgatttgatc
    31321 gtattagctg gatatatgcg aatcattgga ccggtattgc tgaaaaatta tgataaaaga
    31381 atcatcaata tccatccgtc tcttttacct gccttccctg gtttgcatgg catacgtgat
    31441 gcctttgaag ctggagtaaa agaaacgggt gtcacaattc attatatcga ccaaggtgta
    31501 gatactggac caatcatccg gcaggaaaaa gtgagaattg aacaggagga cacgttcgat
    31561 tctttagaag aaaaaatcca tagagtagag catcgaatct atccagaagt gattagtgaa
    31621 attattgaaa atggaggcta tctgaaatga caagagcatt gatcagtgta tcagataaaa
    31681 ctgggattgt agcattcgcc caaggtttga ttgaacaagg agtagaaatc atttcaacag
    31741 gaggaacaaa gcatgcgttg gataaagctg ggatcaaaac gctttcgatc gaagaagtga
    31801 cagggtttcc tgaaatgatg gatggccgtg tcaaaacact tcatcctaaa atccatggtg
    31861 gtcttttagg aagacgcgat ctccctagcc atatggaggc aatggaaacg gaaaatatcc
    31921 aaccaatcga ctttgtttgt gtcaatttat atcctttcaa agaaacgatt atgaaaccag
    31981 atgtcgaaat agcagaagct attgaaaaca ttgatatcgg tggtcctagt atgctccgta
    32041 gtgcagcaaa aaaccatgaa tttgtcacct ctgttgttga tccatctgac tatgaagaag
    32101 tactttcaga attacgtcag tatgggcata cttcactttc tttcagacaa aaactagcgg
    32161 caaaagtctt tcgtcatact gcggcatatg atgcactgat cgctcaatac ttaacggatt
    32221 gggtggaaga aaaagaaccg gaaaaattga cacttacgta tgaacgaaaa caaacgcttc
    32281 gttacggtga aaatagccat caagccgctg ccttttatca gtccgctctt ctggaaaaat
    32341 attcgatcgc agcagcaaaa caactccatg ggaaagaact ttcttataat aatatccgag
    32401 atgcagatgc tgcgctacgg atcatgaagg aatatgacga gccaacggta gtcgccttga
    32461 aacacatgaa tccttgtggt attgggacag catctacgat tttagaagcg tgggaagcag
    32521 cgtatgaagc agatcctgtt tcaatctttg gtgggatcat cgttttgaat cgagaagtag
    32581 atctgccaac agcagaacag atgcataagc tgtttctaga aatcatcatt gccccttctt
    32641 atacacaaga agctttggaa ttactgcaaa aaaagaaaaa catccgatta ctgacagtag
    32701 actttgcgca ggaagaaaat caaaatcgag aagaaacggt ctcagtctta ggtggacttc
    32761 ttgtacaaga tcaagatctg gcaatagaaa atgaagaaga gtggaaagtc gtgacaaaac
    32821 gtaagccgac tccagcagaa gcaaaagcat tagcgtttgc ttggagagct gtcaaacatg
    32881 tgaaatctaa tgcgatcgta ctggcaaacg aacgtcagac agtaggaatc ggagccgggc
    32941 agatgaatcg agtcggttca gtaaaaattg cgattgaaca agcgcaagag aaaatggaag
    33001 gtgcagtttt agctagcgat gcttattttc caatggatga ttcggttgaa tatgcagcca
    33061 aacatggtat aaaagcaatc attcagccag gaggaagcat caaagaccaa gattctattg
    33121 atatggcgaa taaatacgat ctagcaatgg tctttactga tgttcggcat tttagacatt
    33181 agagaggagt cggataaatg aacattttgg tgatcggtgc aggtggtcgt gaacatgcaa
    33241 taagtaaaaa acttttagcc agtccgaagg tggatacggt gtattgtgcg ccaggaaatc
    33301 cggggatgac aaaagatggt atcaattgtg tcgctatcag tgagcgagat cattcagcgt
    33361 taatccattt tgttaagaaa gaggctattg attggagtat cgttggtcct gaagttcctt
    33421 tgttaaatgg gatcgtcgat gattttatgg cggaaggatt gaaaatcttc ggtccgacaa
    33481 aagcagcagc tctgatcgaa ggttcgaaaa gttttgctaa acaattaatg gatacttatc
    33541 aaatcccgac agctaaatcc agaagttttg aatgttacga aaaagcagca gcgtatatca
    33601 aagaaaatgg tgcgccaatc gtagtgaaag cggacggctt agcagctggg aaaggtgtag
    33661 tcgtggctac cacagtgaag gaagcgctgg atgcggcaga acaaatgttg aaccagcatc
    33721 gatttgggga tagtagcaaa aaaatcgtcg tagaagagtt tttggcagga gaagaattct
    33781 cccttttggc gtttgtccgt aataccaagg tgtatccgat ggtcatttcg caagatcata
    33841 agcgggcatt tgataacgat aaagggccaa atacaggcgg aatgggtgct tattcaccag
    33901 taccgcaaat ttcaaaaaag atgattgaaa aagctgtgga agaagtttta aaaccagcgg
    33961 ctttgggaat gaaagaacta ggacgctctt tcacgggtgt tttatatgcg ggtttgattg
    34021 ctacgaagga aggaccaaaa gtgatcgaat tcaatgcgcg tttcggtgat cctgaaacgc
    34081 aagtcgttct ttctcggttg aagagtgact ttgcgcaagt aatcgatgat ttattagaga
    34141 atcgtacagt agagttaaag tggcagcaag aaggatacaa tgtaggcgtt gtggttgcag
    34201 ctgccggtta ccccgaagaa tatgagacag gaatggttct accagatttt ctggaagaag
    34261 agctttctgt ttactatgct ggagtttcct caaataacgg caaactagtt ggagcaggag
    34321 gacgtcttta tttagtagaa gcatacggtg agacgatcaa agaagcacag aaaaaaattt
    34381 acaccgcgtt aatggataaa acgacagatg gaacatttta tcgtacagat attggagaaa
    34441 aagctgccaa tagcgaactg atcgtgaagt aaatcagctg cttccattta taaaaaatac
    34501 aacaaacata atcggaagcg ccgattttag tgaagccttt tcataaagag tgtggtacac
    34561 ttttgttgtg tatataaata aggaggagtt cagatgaaaa aaattcaatt aggtacttct
    34621 gatcagaaag cagctgcagt gatcttaggc tgtatgcgta tcaacaatgc ggaagatcca
    34681 gtaaaagtga ttgagacagc ttatgaaaac ggcattgatt tttttgacca tgcagatatt
    34741 tatggaaacg gtgaatgtga agaaatcttt gcagatgctc tagcaaaaac atcaatcaaa
    34801 cgagaagatc tttttattca aacaaaatgt ggaatcgttc caggtgtgat gtttgatttt
    34861 tctaaaaaac atatcattga atctgtagaa aaaagtttga cccgtctgaa aatggattat
    34921 gtggatgctt tacttcttca tcgtccagat acattggtag aaccagagga agtagctgaa
    34981 gcgtttgatg aattggaacg gtcaggaaaa gttcgttatt ttggcgtcag caatcaaaaa
    35041 ccaatgcaaa tcgaattgct gaaaaaatat gttcgtcaac cattagtagc taatcagctg
    35101 caatttggct tgaaacatac tggaatgatc gatcaaggga ttcatgtcaa tatgacagat
    35161 gaagcaagta tcgatcatga cggtagtgta cttgattatt cccgattgca tgatatgacg
    35221 atccaagcat ggtcaccata ccagtatgga ttttttgaag gtgtatttat cgggaacgaa
    35281 aaattccctg aattaaacca aacattagaa gaactagcag ctaaatatca aacgacacca
    35341 actggcttgg cttctgcttg gattttacgc catccagcca atatgcaagt gatcgccggt
    35401 acgatgaacg tagggcgaat caaggaaatc gctcaagctt cagaaattaa tatgtctcgg
    35461 gaagattggt acgaattgta ccgtgctgct ggaaacattt taccttaaag attgtcataa
    35521 gcaggtcaaa agggctataa attaaaaaga cagaataact tgaaagctgt tttcagcttc
    35581 gagatattct gtcttttttt attgcaaatg gaaagagtat cattgtacgg atttcgttta
    35641 ttagttattt ttgtttttaa ggaaaagctg tgtccgatat tcttttggag aaatcccttg
    35701 ttttttctta aataaataac tgaaatagtt agggtcatta taaccaattt caaaagcaat
    35761 atctgaaata cgatagtctg attgctgcaa taattttttt gctaattcga ttctttgttc
    35821 tgtcagataa tcgatgaatg tttggttaaa cgattgagaa aaaatcgtac tgaaatgaga
    35881 gggacttaat gcaacttgtt gagcgaccca agttaaggac atatctggat cggtaaaatt
    35941 ttctttgata aaatccaatg catggttcaa aattggctga tatttgttcc ttgaaggctg
    36001 tgagggcaga gcagttaaga aagaaatgaa catataaact gtcgaacggt aaagcgagtc
    36061 gtcggcgctg attgctaagc gttgactggt ggatgtgcaa tgaaccattt cttgcgggat
    36121 tttcttttct ttcaggcaga catgctggag tagcttagtc aagacaaata aacgatataa
    36181 tcgtgttcga gttggtgtgc cttgtggttt cgtcaagtgt tcgatgtact tagatacttc
    36241 gtttttcgat aatgaagaca aatcaagtga gaggtcaaag taaaagacag aatcaactgg
    36301 aagtttcgct tcttgaatca aatcctcata gctgataatt tgttctttaa gggaactggc
    36361 ggtttcaagt atcatctgcg tgatctcaaa agatgcaggt aattcgctga ttctttctgc
    36421 gacaggacca atggcgacag aaaaattatt tttttgtccc ttttctaatt catggaccaa
    36481 cgtttgggct acctgataac tggtctcgat cacattttct tcaagagaat caactattaa
    36541 aaatttgatg aatcgagagg aaacagcaga gaagatcact tgttgattct ccctaaataa
    36601 aaacttcaaa taatcactga gatgagcgta atcttcaaag tctgtactga cttggtttgc
    36661 tgctaataat acggtcattt ttttccctgc taaagagcga ttgaaagatt cagcaagttt
    36721 caatgtttca ctgatcgtaa gtgagccttt gaacaaaccg ttcaagaaat gatttttttt
    36781 caattcgaaa acaagatcag aagaagtgtg cgtttctttt tgctgcaaat ctaaagcagt
    36841 tactgctttt tctattgctt gtttcaattc gctactttta atcggtttaa gcagataatc
    36901 atctgcctgt acctggattg ctcctttcat ataatcgaaa tcgtcgaatc cactaataaa
    36961 tatgagacga gtccaaggaa gtagtttcct tgcttctttt gaaaaagtca acccgtccat
    37021 gaaaggcatt tggatatcag tcagaatgat gtcgggctgt aagtcgataa ttgaagacag
    37081 tgccatctca ccatctccag cttcgccgac ataagtgatt ggataataag aaccaaggac
    37141 agttatttgt tcccgcaagt tattgcgaat gaggtattcg tcttcgatga taaaaacttt
    37201 atacatgttt gtttcctcca attgttttgg gagatagagg gatcatcagt gtgacagttg
    37261 ttccttctcg gtatttgctg tcaatcgtga taggtttttg atcatttccg taataaagaa
    37321 ggagacgctt gtttacgttg taaagtccgt agccgatact gaaatctgaa tcgatccctt
    37381 tggctaactc tttacgtatt tctgccagtt tttctgcagg gatgccaatc ccgttgtcta
    37441 taactttgaa ttggatcatc tcatcaattt tgcatgcggt aatacgaata aatcctttgt
    37501 gacggacgta cttcgttcca tggtatacag cattttcaat caatggctgc aagatcattt
    37561 ttaaaataga aacaggaaaa agtgtttctg gtatatcgat cttatagtca agcatatggc
    37621 cgtatcttat ttttaagatg actagataat cattgatatg gctaatttct ttttcaagag
    37681 gaatccagtc ttgtcctttg cttaaagacg tacgaaaaaa gccggaaagt gcatgggtca
    37741 tagtcagagc ttcttcaaag cagtcttgtt cgatcaaaga aataatggca tccaaactgt
    37801 tataaataaa atggggagta atctgagctt gcaatacacg aacttcactt tgtgctaaat
    37861 ggtattgttt caaagcgttc tcccgtaaag actggtttag atcttccgcc attttattca
    37921 tgctagcggt aagtgtttgt aattccagtg tttccggcag ggttaaacga aagccaaggt
    37981 tccctgaaga aagttgttct gacatctcga tcagctgatt gatcggttct tggacctttc
    38041 tctctaaaaa gcgacgattc tttttcgcat agaaaaaaat ggtactaaga actccaagtt
    38101 ccactattgt taatagaatg agcgagcgga caagtgtcgt attttttttg cttgctaagt
    38161 cgatctccac acgaacaaaa tcttgtaaga tatctgacag cagttgagtg acagaatcta
    38221 cctgattgat gatctcttca ttggtatcca taaaatcttt tgccaagata ttattgatca
    38281 gctcgttttg ataatcttcc agtgtatcga tcgtacgtag cgatacatct aacgtactaa
    38341 tttctttaag tgtatttgta ttatttttga tctctcgaat atcattccta agttcttcta
    38401 ggatattttc ctcattgaac tcatctacgg ttacttggcc agaaatcaag tcccacatac
    38461 tttccaaaac tttttgatca accttcaaag caattgcatc tgctttttgg atatttgcga
    38521 tcgtctgttg ataaatcaaa agctgttgcg aatataacag tatgctcagt agcaaaggga
    38581 taacagcaaa tagaagcatg gctttgtttg cttgggcaaa aatctgtttt atgcttaaat
    38641 tttttgtcat gatacttctc cagtcaataa tttctggttg ggatgttagc tatattctgc
    38701 tgggaaaaga cagtctcgga aataaaaata tctttaggta atgtttttcc tgtttgaata
    38761 tttccaaaat aacgagtaac ggtattggct acataccagc cgacatcagg tttacactcg
    38821 actacacaat tgacgattcc tgagcgtagc ttctcgatca tatcttcttg tccatcaata
    38881 gttacaatga tcaaatcttc tccaggtttg attgatgttt cttttatcgc agctaaagca
    38941 ccgtgagtca tttcatcact atggctgtat aatacatcga tttcggataa atcatttgtt
    39001 tctaaatatc gtttcatctg gtcttttcct ttcatccgaa taaaatcacc gacaattcgg
    39061 tgaacaatag tcatcggagg tttatggtcg atggttccat ctttttttag ggttggttta
    39121 cgattgattg attccaaaaa tccttcggag cgtagcttgg tcggtgtgga gttctctgaa
    39181 ccagcaagtt ctaggattct gatttcctgt ttctttgaat ttgtgaagtg attgctgaca
    39241 aataaacccg cccgatttcc ttctgctttg aaactggggc caatgtgggt gacgaaaagc
    39301 tcgggatcat tcgtttgaat atgtctgtcg acaagtatga cagggatccc tgctcgcttt
    39361 gcttctttta atacagagtc ccacccggat tcttgcaaag gagtaaaaat gatcatatcc
    39421 actttatagg caatgaacgt ccgtatatct tggatttgcc gttcctgatt catgtagcca
    39481 tttcggtaga gaacttggta atcttctttt tcaagcgcag tgataattga ttcagtatgc
    39541 tttttccgcc agctactctc cgttcctgat tgcgaaaaac caacgactag tgggtcaggt
    39601 gcttcctttg tattaggtgt acaccccatc atttggtaag cgattacaac aagggctaaa
    39661 aaaaataaaa cacgtttcac tttaaatcac tccatctgga tttgttagta ctatcttatc
    39721 agataaaagg gaaaatcaaa gtcaaaagga atcaatcgaa aaaaatccaa gaaacttaaa
    39781 aaaatccaag acaaaaaaag tacacctaaa aaatttaaag taaatcccat aaaaaaatca
    39841 atgttaacgc tttctttatt agctatactc ggtgtatcaa ttgaaatggg ggataaaaaa
    39901 tgaaaaacaa atttagaaag attgttggga ctatcgctat tttgaccgca agcattctat
    39961 taagtgcgtg tggcaattca ggatctgcag atgatagtac ggggtatgta ggaatcgcta
    40021 tgccgaccaa atcagcagaa agatggatcg ctgatgggaa caacatggtc tcggaattag
    40081 aaaaactggg ttataaaaca gatcttcaat atggagaaga taaagtagaa aatcaagtag
    40141 cacaaataga aaatatgatt acaaaagggg tagatacact tgtgattgct tctatcgatg
    40201 gttcggcttt aactgatgtg ttagctaaag caaaagaagc agatatcaag gtgatcgcct
    40261 atgatcgttt actgatgaat tcagaaaatg tcgattatta cgcgactttt gataattttg
    40321 gagtaggtgt atcgcaagca gcttatattg aagagcactt aggattgaaa gaaggaaaag
    40381 gaccattcac gattgaatta tttggtggct cgcctgacga caacaacgca ctaatcaatt
    40441 acaacggagt gatgtctgtt ttacaaccat atatggacaa cggtcagttg gtcgtgccat
    40501 ctggacagac gagtttcagt caaatcgcta cgcttcgctg ggatggttct acggcgcaag
    40561 caaggatgga taatctgtta agcgcaaatt atactgacca aacattggat gctgttttgt
    40621 cgccatatga tccaataagt ttaggaatca tttcgtcatt aaaaggcgtg gggtatggga
    40681 gtgaaagtaa acctttacct gtgattactg gacaagatgc gacagttgct ggcgttaaat
    40741 cgatcattgc cggggaacaa acacaaacga tctttaaaga cacacgtatc ttagcaaaaa
    40801 atacgattga aatgatcaaa gcaatcagtg atggagaaga ggttccggtc aatgatactg
    40861 aaacttatga taatggggtg aaaactgtcc ctacgtattt agcaaataca gtatctgtcg
    40921 ataaagataa ttatcaagca gagctgatcg acaccgatta ttataaagaa tccgatctga
    40981 aaaattaaga ggaaccaaac aaggaactaa agagatctaa gtcagagaga ggataagaat
    41041 tatggcggat tgtattttaa aaatggcaga tatcaccaaa aaattttctg gaatccgagc
    41101 gttagattgc gtgaatctga tgattgaacg tggggaaatc catgcattgt gcggagaaaa
    41161 cggagcggga aaatcaacat tgatgaatgt cttgagcggc ttgtatccat atggctccta
    41221 tgacggggaa atcttttatg atggaaagct gtgtcaattt aaagatttga aagatagtga
    41281 agaaaaaggc atcgtcatca ttcatcaaga attagcacta agtccttatc tttcaataaa
    41341 agagaatatt tttctcggaa atgaacaagc agaaagaggc gtgattgatt gggaactgac
    41401 agaaaagaaa acatatgaac tgctgaataa agtcggtttg tctatcgacc cgaatcttct
    41461 agtatcccag attggcgtag ggcaacagca actagttgaa atagctaaag cattatcaaa
    41521 atccgtccgt ctgttgattt tggatgagcc taccgctgca ttgaatgaag aagaaagcgc
    41581 gaatttgctg agattgatcc gagagttgaa agagcaaggt gtaacctcca tcattatttc
    41641 acataagctg aatgaaatcg tggatatcgc agatcgaatc accattttac gggatggaca
    41701 gacgattgag acactagaaa atgaaatgat cagcgaagag catattattc gcgggatggt
    41761 gggcagagat ttagttgatc gttatcctga aagaaaaccg gatattggag aagtttattt
    41821 taaagtagaa gactggacgg tctatcaccc tcttgatcac gagcgaatca taaacgacca
    41881 catcaacttg catttgaaaa aaggagagat tgtaggaata gctggactga tgggagcggg
    41941 cagaactgaa ttcgctatga gtctatttgg acattcttat ggaagcaaga tatccggacg
    42001 tatattaaag gaaggggcag agatatcagt taagaccgtt ccttcagcca ttgcaaacgg
    42061 actcgcttat gtctcggagg accgaaaatc tttaggactg aatctgctga tggatattcg
    42121 ggaaaacgtg acgctgagca gtttggataa aataagtcaa aaaggagtat tagataaaga
    42181 aaaagaagta actgaggcag aaaatttccg gaaaaagatg cgtataaaaa caaactccat
    42241 ccatcaaatt gtcagtagtc ttagtggtgg gaatcaacaa aaagtcgttt tatccaagtg
    42301 gctgatgaca caacccgata ttcttttttt ggatgagcca acccgtggga tagatgttgg
    42361 ggcaaaatat gagatctaca cgattatcga agaaatggca gcaaatggca aagctgtctg
    42421 tcttatctct tctgaacttc ctgaaatatt agggatgtgt gatcgaatct atacgatgaa
    42481 cgaaggacgt attacaggag aagtctcgcg agaagatgca gatcaggaag tcttgatgaa
    42541 attaatgacg aaagaagagg tgggttaagt tggaacaggc aaaaaatctt acggataaaa
    42601 aagagaaaaa actaaatttg aaaagtagag cagctgatct ttttggtcgc tacagcatgg
    42661 tgatcatttt agcgggacta cttattgcgt ttcaattgat gacggatggt attttctggc
    42721 gtccattgaa tataactaac cttgttctgc aaaatagcca tatattagta ctagctgcag
    42781 ggatgttatt agttgtgctc ttaggtcatg tggatttgtc ggtagggtca gtgatggcgt
    42841 ttgttggtgc aattgcgggc atgatgatgg tcaatcgcca aatgaacccg tggatgacag
    42901 ttatcgcatg tcttgcagta ggcgggctga tcggcgcatg gcaaggtttt tgggtagctt
    42961 atgcagggat accggcgttt atcgtgactt tagcaggatt gctgatgttt cgtggactga
    43021 cacaagttgt tctaggaggc caatctctag caccttttcc agctgttttc caaaaaatgt
    43081 ctacgggtta tttaccagac ttaagcgaag gaagcagcct tcacttcttg acattgattt
    43141 taggaatgat catcacgatc gtgttagttg ttggtcaatg gcgaacaaga aatcgtcgga
    43201 aaaatcattt atttgaagtg gaatctccaa cagctttctt ggtaaaggcg gtatttacag
    43261 cagtcgttgt actaggaata acttatatct ttgcctctta tcaagggtat ccgattattt
    43321 tgatcattct tggtttgatc gttgcagcat atgggttttt gacaaataaa acagtagcag
    43381 gtcgacagat ttatgcgacg ggcggaaatc gaaaagcggc tgaactatca gggatcaaaa
    43441 caaagcgaat cacgttctgg gtattcgtca acatgggagt catggcagct ttagctggat
    43501 tagttttagc tgctcgattg aatgcggcaa cacctcaagc aggtacatcg atggaactgg
    43561 atgcaatggc ggcagtatat tttggtggtg cttcgacttc cggcggcatt ggaacaatca
    43621 tgggtgcgat tgtcggtggc ttggtcatgg gtgtattgaa caatggcatg tcgattttag
    43681 gcgtcggggt agactggcag caggcaatca aaggattgat cttgctttta gccgttgtgc
    43741 tagacttgta taataagaaa aaacgaacaa cgtaggagaa cgccggttat tcagaaaaaa
    43801 gactgtgaca agacatttgt cacagtcttt ttttcgctaa aagcgaacat tcattgtttc
    43861 gtttgtatag actaaaaata atatatgttt ttttaccatt cgtataaatt ctctttttgc
    43921 tcttgatttt tttgcttaat tgagttaaat tacatagggc gctaaaaaaa gccttgatta
    43981 aattaataat gttcgtgttt tgctggatgt gaacatcagg aggataatgt aatgaaagta
    44041 tttgattacg aagatattca attaattcct aataaatgta tcgtaaatag ccgttctgaa
    44101 tgtgatacaa cggtcacatt aggtaaacat acattcaaaa tgccagtagt gcctgccaac
    44161 atgcagacga ttattgatga aacaatcgca gaattcttag cggaaaatgg ttatttctat
    44221 atcatgcatc ggtttgatga agcagctcga attccgttta tcaaaaaaat gaaaaagcgt
    44281 ggattgatta cctcgatcag tgttggcgtc aagaaagagg aatattcctt tatagagaag
    44341 ttggcagaag aatcgctaaa tcctgattat atcactatcg acatcgcgca tggacatgct
    44401 aactcagtta ttgatatgat tcagcatatt aaaaaatatt tgcctgaaac ttttgtgatt
    44461 gccggtaatg ttggtacacc ggaagccgtc cgtgaattgg aaaatgcggg cgcagatgca
    44521 acaaaagtcg ggattggtcc aggaaaagta tgtattacaa aaatcaagac aggctttggg
    44581 actggaggct ggcaactggc agctcttaga tggtgtgcga aagcagctcg caagccgatc
    44641 atcgctgacg gtgggatcag aacacatggc gatattgcaa aatctgtccg ttttggtgcg
    44701 acaatggtca tgattggttc attgtttgcc ggtcacgagg aatctcctgg cgaaacaaaa
    44761 gtcgaaaatg gaatcgtgta taaagaatac tttggttctg cttcagaatt ccaaaaaggc
    44821 gaaaaacgaa atgttgaagg aaagaaaatc tggatccaac acaaaggatc tttgaaagat
    44881 acactagtgg aaatgcaaca agaccttcaa tcatctattt cttatgctgg aggaagagat
    44941 ctggaagcca ttcgaaaagt tgactatgtc atcgtgaaaa attcgatttt taatggagac
    45001 acaatctaaa aaataaataa aatattagga tgttgtgaaa aagttgtcct gcattaagta
    45061 ataagaacag ccaaacaaaa atagttcttt atatgttttg tttggctgga attattatcg
    45121 cagatacaag cttttgaaca tcattattca gaataaggga ctgtgataag atttttgtca
    45181 cagtccctta tgttttgcaa aaaaagtttt tcttttttct cgtaaattcg gtaaaatcaa
    45241 aagaaaaaat agacaataga aaagagaagc ttatgtggga aaaagtagag aaaaaacaag
    45301 gaccgattta tcgtcaaatt atggaacaga ttatgagaga aatcgaaaac ggaaggatca
    45361 atcctggaag tcaacttgaa tcagaacgaa aactagggca actttttggt gtgaatcgtt
    45421 ccaccgttgt ccatgcattg gaagagctta gggagcttgg ggtactaacg agcaaacgtg
    45481 gcagcggatg gtttgttaat cagactgaat gggggaaatt cgctgttcca agaatcgatt
    45541 ggcggcaaat gatctctcct cgttatgagc agacggactg gtatgagcaa aaaataagag
    45601 aagcaaagca gatccaaaaa acatcgtttc ttgatttata cgctagcgag atgccagatg
    45661 agcttttgcc gaattttgag tttccttctt attctcttga agaaatctta gcggaagaaa
    45721 aagaaatgga tcttttaggc tatactccgt tacgtaacaa aatcaaacgg tatttggaaa
    45781 gtaaatttga attggcattt tcccccaacc agcttcttgt gactggtggc ggtcagcaag
    45841 caatcttttt gattttacaa acgattcttt cagctggaga ggggatagct gtagagtcac
    45901 cttctttttt ttaccgatta cctcttttca aagcttcagg aacgcgttta tttgggatac
    45961 cgatggatga agaagggatc gacttagaaa aactgaaaga atcgatccat aaaaataaat
    46021 tgaaagcagt gcttatcaac ccgaattttc aaaaccctac aggaaaagtg atgagtcaaa
    46081 aacgacgaga aaagctagta gaattgtgtc gacagtatcg gcttccaatt attgaagacg
    46141 atgtatttac ggatctttca tttctaaacc aagaagaagt ccttcctata cgaacgattg
    46201 atccggataa tgtgttatac gtcggttcgt tatctagggt tttaggaaaa acaacgaaaa
    46261 tcggttgggt gataggacct gagactttta tctcgcaact agctcaagcg caagaaatga
    46321 tggagttttc gatgaatatc cttacacaaa tcacagtggc taatgtattt aatgaagcaa
    46381 ttgatttgaa aatgcaagat gtaagaaaaa agttgaaaga aaaaagccga ctgataagcg
    46441 cttggggaga ttcacaatcg tttttcaagc ttcattcaat tttaggtggt tactatgcct
    46501 ggattacttg ggatgggaaa aaactagacc ccgaactagc agaaaaagta gtagatatgg
    46561 gtcttggaat tgcaccaagt ctactttttg ggcaagatat aaatggaatc agaatcaatt
    46621 acagtaggct ggatgaaaac caaataccgt tgttttattc aaagatgaaa attctagaaa
    46681 agtggttaac agaataattt cacaaagtaa atagattaaa gaatataaca gcttggattt
    46741 aaatttcaag ctgtttcttt tttttcggaa aaagaatacg atataaagaa actaagaaaa
    46801 aaccattatt tgtacgtccg ttcacgatgt gtaaataaaa taaatgtttg caacaaaact
    46861 gtataacacc aacgttttta aatagaaaca gatggttgta agtttgatta gaaaatagaa
    46921 aaaatgcaga tgaaaaataa aacgctttcc ttattgtgat tttattcact aatgctgtta
    46981 atatgcggat tggcataatt tcctttcatc gtgaaaattt gtgcttttag cttgtatttc
    47041 tcataaatcc atgataattt attaatgaag acggttaaat agtgatacag ttttattcac
    47101 tgtttcaatc gcagaatgaa gagattttct tatagatgag agaggaatgt gtgacaaatg
    47161 gcaaacaaaa aagcaccgat cgacttccaa gcactgctag atgaagtcaa tgcagatttc
    47221 ccagtatatc aagcacttga tcaagatgga aacgtagtta acaaagattt agtacctgat
    47281 ttatccgatg atgaactcgt tgaattaatg actcatatgg tatggtctcg tgtattagac
    47341 caacgttcaa cagcgctgaa ccgccaagga cgcctaggct tttttgcacc aactgctgga
    47401 caagaggcaa gtcagctggc tagtgcttac gcatttgata aagaagatgt tcttcttcca
    47461 ggttatcggg atgtacctca actagtaaaa catggtttac ctttatctca agctttttta
    47521 tggtctcgag gacatgctgc aggtaattct tatccggaag aattgaaagc tctaccacca
    47581 caaattatca tcggtgcaca atatgtacaa gcagccggtg ttgcattagg tttgaagaaa
    47641 cgaaacaaga aaaatgtcgt cttcacttat actggtgatg gcggttcatc acaaggtgat
    47701 ttttacgaag caatcaattt cgctggcgct taccatgcaa atgccgtatt ttatatccaa
    47761 aacaacgggt atgcgatctc tacacctcgt gaaaaacaaa ctgcagcaaa aacacttgca
    47821 caaaaagctg tcgcagctgg gattcctggt attcaagtcg atggaatgga tccgcttgca
    47881 gtatatactg tatctaaaat ggcacgggaa tgggcgatca gtggaaatgg accagtccta
    47941 atcgaaacac tgacttatcg ttatggacca catacactat ctggtgacga tccaacccgc
    48001 taccgtacaa aagacacaga tgacgattgg cagaaaaaag atccattgat ccgcttccgt
    48061 aaatatttga cggaaaaagg actttggtct gaagaaaaag aagaacaagt catcgaagca
    48121 actaaagaag aaatcaaagc agctattgct gaagccgaca aagtaccaaa acaaaaggtt
    48181 tcagatttct tgaaaaacat gttcgaagta tctccacaat caatcaaaga acaaattgca
    48241 atttatgaag cgaaggagtc gaaataatca tggcacaaaa aacaatgatc caagcaatca
    48301 cagatgcctt agcacttgaa ctagaaaatg acgagaatgt cgttgttttt ggtgaagatg
    48361 ttgggaaaaa cggaggagtt ttccgggcaa ctgaaggatt gcaggaaaag ttcggagaag
    48421 accgagtatt cgatactcct ttagctgaat ctggaatcgc aggattgagt ttcggtcttg
    48481 cgttagaagg atttcgtcca gttcctgaaa tccaattctt tggttttatt ttcgaagcaa
    48541 tggacgaagt agtagctcaa atggctcgta caagatatcg aatgagcggc actagaaatc
    48601 tacccatcac aattcgttcg ccatttggtg gcggtgttca tacaccagaa ttgcattcgg
    48661 ataatttaga aggtttgatc gctcagtctc ctggtattcg agtagtgatt ccttccaacc
    48721 cttatgatgc aaaaggacta ttgatttctt ctattagaag taacgatccg gttgttttcc
    48781 tagaacacat gaaactatat cgctcattcc gagaagaagt gccggatgaa gcttacgaag
    48841 ttcctttgga taaagcagca gttactcgtg agggaacaga tgtatctatt atcacatacg
    48901 gagcaatggt gcgtgaagca atcaaagcag cagacaatct tgcaaaagaa aatatatctg
    48961 ttgaaatcat tgatcttcgt acagttgcgc cattagatgt agaaacgatc attcaatccg
    49021 tggaaaaaac tggtcgcgta gttgttgtcc aagaagcaca acgacaagcg ggcgttgccg
    49081 cgcaagtagt ttctgagatc tcagaacgcg ctatcctttc attagaagcc ccaatcggac
    49141 gagtttctgc acctgataca gtcttcccat ttggacaagc agaaaatgtc tggttgccaa
    49201 atgcaaaaga tatcgaagac aaagtaaaag aaatcgcgga attttaagaa aagaagggac
    49261 gaaacaaagt catggcttat caatttaaat tgccggatat cggtgaagga atcgcagaag
    49321 gtgaaatcgt caaatggttc gtcaaaccag gagatacgat caatgaagat gatacattac
    49381 tagaagttca aaatgacaaa tcagtagaag aaatcccgtc acctgttaca ggaacggtta
    49441 aaaatgtgat tgttccagaa ggtaccgtag caaatgttgg ggatgtatta gtagaaatcg
    49501 atgcaccagg acacgaagac aatgaagggg acagtggtgt agccgcagaa tcccaaactc
    49561 cagctaaacc ggctgctgaa cccactgttg atacagaatc agctggatcg tcaagcgaag
    49621 gagtcttcca atttaagtta ccggatatcg gtgaaggaat cgcagaaggt gaaatcgtca
    49681 aatggttcgt caaaccagga gatacgatca atgaagatga tacattacta gaagttcaaa
    49741 atgacaaatc agtagaagaa attccgtcac ctgtcacagg gacagttaaa aacgtgattg
    49801 ttccagaagg cactgtagca aatgttgggg atgtattagt agaaatcgat gctcctggac
    49861 acaatagtgc accgtcaact agtgcgccat cagctgaagc accaaaagaa aaagtggaaa
    49921 catcaggttc agcaagtgtc gttgaagctg ccgatccaaa taaacgtgta ttggctatgc
    49981 catctgttcg tcaatttgcc cgtgaaaaag atgtagatat ctcacaagta actgcaactg
    50041 gaaaaggtgg cagagtaaca aaagaggaca tcgaaaactt cttggcaggt ggtccaagca
    50101 gtgcaccagc gaaatcagaa gcgccagaag cggcagcacc gaaagaagca gctcctgcag
    50161 cagaaagcaa accagctgca ccagcaaaac cattcaagtc caacttagga gacttggaag
    50221 aacgtgtagc aatgacgcca acacgtaaag ctatcgctaa agcaatggtc aacagtaagc
    50281 atacagctcc acacgtgaca ttgcatgatg aagtagaagt gtctaaacta tgggataatc
    50341 ggaaacgatt taaagaagtt gctgctgcaa atggtacgaa acttactttc ttgccatatg
    50401 tcgtgaaagc tttaacagcg actgttaaga aatacccagt attgaatgca tctatcgacg
    50461 atgcaaacca agaaatcgtt tataaacatt actacaatat cggtattgca actgatacgg
    50521 atcacggact atatgtacca aatgtgaaag atgcagaccg caaaggaatg tttgctattg
    50581 cagacgaaat caatgagaaa gcaaaattag ctcatgacgg caaactatct gcagaagata
    50641 tgcgcaatgg aacaattacg atcagcaata tcggatctgt aggtggcggt tggtttacac
    50701 cagtcatcaa ctatccagaa gtagctattt taggtgtagg aacgattgca caacaaccaa
    50761 tcgtaaatgc tgaaggcgaa atcgttgtcg gtcgtgtgat gaaactgtca ttaagtttcg
    50821 atcaccgtat cgtagatgga gcaactgcgc aacaagcaat gaataatatc aaacgtcttt
    50881 tagctgatcc agagctatta atgatggaag gatgataaac aaatggtagt tggagatttt
    50941 gctgtagaat tagatacagt tgtaattggt gcgggacctg gtggctatgt agctgccatc
    51001 cgcgctgctg aaatgggaca aaaagtagca atcattgaaa gagagtatat tggcggtgtt
    51061 tgtttgaatg tcggctgtat tccttcaaaa gcactgatag ctgcaggaca ccattatcaa
    51121 gagtcactag attctgaaat gtttggagta actagtgaaa acgtcaaact agattttgca
    51181 aaaacacaag aatggaaaga aaacaaagtt gtccatacgt tgacttctgg tgttggtttc
    51241 ctattaaaaa aacataaggt tgaaacaatc gaaggagaag cgttcttcgt agatgatcac
    51301 acactacgcg tcattcatcc agattcagct caaacttatt cgttcaataa cgcaatcatc
    51361 gcaacaggtt cccgtccaat cgagattcca ggatttaaat tcggaggacg tgtgttagat
    51421 tctactggcg gattagcatt gaaagaagta cctaagaaat ttgtgatcat cggtggcggt
    51481 gtgattggtg ccgaattggg cggagcgtac gcaaacttag gagcagaagt aacgattctt
    51541 gaaggctcac cacaaatttt accaacttac gaaaaagatc tagtaaaatt agtagaagat
    51601 gatttcaaga aaaaaggcgt aacagtcgta acgaatgcaa tggcgaaaga agcagtcgat
    51661 aacggtgaca gcgtgacagt gaaatatgca gtggacggga aagaagaatc cgttactgct
    51721 gattatgtga tggtcactgt aggtcgtcgt ccgaatacag atgatatggg cttagaacaa
    51781 gcgggtgtcg aagtaggaga acgaggattg atcacggtgg ataaacaagg acgtacgaac
    51841 gtacctaaca tctatgccat cggtgatatc gttccagggg cggcattagc acacaaagcc
    51901 agttatgaag caaaaatcgc tgctgaagca atctctggca aaaaagtagc tgtagattat
    51961 aaagcaatgc cggcagtagc cttcactgat cctgaattgg cttctgtagg tatgacgatc
    52021 aaagaagcaa aagatgctgg tatcgaagca aaagcatata aattcccatt ttcagggaac
    52081 ggtcgggcat tatcgcttgg taaaacagaa ggattcatcc gtctagttac gacgatcgaa
    52141 gataacgtgc ttattggcgc gcaaatcggt ggagtcggcg caagtgatat ggtctctgaa
    52201 ttagctttag caatcgaatc aggaatgaat gctgaagata tcgccttgac gattcatcct
    52261 catccatctc ttggcgaaat cgtcatggat gcttcggaat tagcattagg tttaccaatc
    52321 catatttgat ttgaataata caggcgaaac gcaactgaaa aatcagttgc gttttgtttt
    52381 ttagaattat caataatatt attatgtaaa cttaaaaata tgacaacttc ttggtttatt
    52441 accacttcct atgctacact gaagaggaat atgaaatagg agagttgaag atgcaagaat
    52501 atcagtatcc acttgattta gattggacaa cagacgaaat ggtcattgta atgaagatgt
    52561 gggaagatct ggaacgtgcg aatgaaaaag ggataaacaa tgaagctttt ttgaagactt
    52621 atcagcagtt taaaactgtt gtcaaatcca ttggtgagga aaaacgattg ggacgggaat
    52681 ttgaaaaagc ttccggttat tctttatatc gtacagtgca gcaagcaaaa aaaaatccaa
    52741 acaaaacttt gagaatgaac gggtgagata aatggaacag atgattgatc atatcaagtc
    52801 ctggcttgaa gaagctggtg agaaaatcaa atcttctcaa caaacattaa caatctcgca
    52861 aaaatcaaat cgaacggatc tagtaacaaa tgtcgatcga aacatccaga attttctaat
    52921 cgaaaaaatc cattcctttg atccagaggc gaaaatattg gcagaagaag aagggtgtaa
    52981 tcaattatcg gatctcgatg gaagagtctt tatcatcgat cctattgatg gcaccttgaa
    53041 ttttgtgatt gaaggggaaa atttttgtat tatgctcgct gtttatgaat ccggcgttgg
    53101 aaagcttggt ttcatttatg acgtgatgag aaatgaactt ttttggggtg gaaaaggaat
    53161 aggcgtgtat aaaaatgatg agccattgcc tagaccagca aataaatcct tatcggaagg
    53221 cttacttggt gtgaatagtt atcttttcgg tcataataaa tatggaatcc gagaaattgc
    53281 ggaaagcagc atggggacac gtatgtacgg atgcgctggt cttgaactag tggcaatctt
    53341 gaaaggaaac cacattgctt atatttcaaa ccttagccct tgggattatg cagcgggaaa
    53401 tgtcttattg gaagagtttg gtatggtaac aagcggtctt tttggtactc ctttgacatt
    53461 tgaaaaaagg gaatactatt tagcagggac ttcttgtgca tatgcagaaa tcgcagacct
    53521 tttgaaaaaa gaaggctgaa aaaaaggtag aaatcattca caaaaatgat aaaaacgagc
    53581 aaaagattaa ttttctcgaa aaaatgaaaa cattttacta tatttcatga aaaaagtctg
    53641 ctataattag cagtcgagta aaatttgccc tgtcataaag aaccaataaa aaaggagtta
    53701 gttacattga attatagaaa cgatatccgt aacgtcgcaa ttattgccca cgttgaccac
    53761 ggaaaaacaa cattagttga tgaactatta aaacaatcag atacattaga cgcacatacg
    53821 caattgcaag aacgtgcgat ggactcaaat gcgctagaaa aagaacgtgg tatcacgatt
    53881 ttagccaaaa acacagctgt agactataaa ggtattcgcg taaacatcat ggatactcca
    53941 ggacacgcgg acttcggtgg cgaagtagaa cggatcatga aaatggttga cggcgttgtg
    54001 ttggtcgtag atgcgtatga aggaacaatg cctcaaacac gttttgtatt gaaaaaagca
    54061 ctggaacaac atatcacacc aatcgttgtc gtaaacaaga ttgacaaacc ttctgctcgt
    54121 cctgagcatg tcgtagatga agttcttgaa ctattcatcg aattaggagc agatgatgat
    54181 cagttagact ttcctgtgat ttatgcatct gcactaaatg gtacgtcaag cttgtcagat
    54241 gatccagcag atcaagaacc aaccatggca cctatttttg atacaattat tgaaaaaatc
    54301 cctgctcctg tcgataacag cgacgaacca ttacaattcc aagtttcatt acttgactac
    54361 aacgattatg taggtcgtat cggaatcgga cgtgtattta gaggaacaat caaagtagga
    54421 gaccaagtag cattaattaa attagacgga acagttaaga aattccgtgt aacgaaatta
    54481 tttggtttct ttggactaaa acgtctagag attcaagaag caaaagctgg agacttgatt
    54541 gctgtctcag gtatggaaga tatctttgtc ggtgaaacag ttactcctgt ggatcatcaa
    54601 gatgcgttac ctatccttca cattgacgaa ccaacattgc aaatgacttt cttagttaat
    54661 aattcaccat ttgccggtcg agaaggtaaa ttcgtaaccg ctcgtaagat tgaagaacgt
    54721 ttgatggcag aacttcaaac agatgtatcc ttacgagttg aaccaacaaa ctctccagat
    54781 gcatggacag tttctggtcg tggagaattg catttgtcta tcttgattga aaacatgcgc
    54841 cgtgagggat acgaattgca agtatctcgt ccagaagtta ttgaaaaaga aatcgacggt
    54901 gtaaaatgcg aaccgtttga acgcgtgcaa atcgatacac cagaagaata tatgggtagt
    54961 gttatcgagt ccttgagctt acgtaaaggt gaaatgcaag atatggtcca tacaggaaac
    55021 ggacaaatcc gtttgacatt cttaacacct gcccgcggtt tgattggtta ttcaactgaa
    55081 ttcctttcaa tgacacgtgg ttacggaatc atgaaccata cttttgacca atatctgcca
    55141 atgcttcctg gtcaaattgg cggacgtcac caaggggcgc ttgtttcgat cgatacagga
    55201 aaagcaacga cttattctat catgagtatc gaagaacgcg gtacagtctt cgtagaacca
    55261 ggtacagaag tatatgaagg aatgattatc ggggaaaaca gccgtgataa tgatttgact
    55321 gtcaacatca cgaaagcaaa acaaatgaca aacgtacggt ctgctactaa agaccaaaca
    55381 tctgtcatca aaaaaccaaa acaattgacg ttggaagaat ctttggaatt cttgaatgat
    55441 gatgaatatt gcgaagtaac accagaaagc attcgcttga gaaaacaaat tttagagaaa
    55501 aatgctcgtg aaaaagcatc taaaaagaaa aaataatctg taaaaggatg aagagagaaa
    55561 ccagcttgtt ataaaaaata gctggtttct ttttttgtcc aactcttttc tgtggagaag
    55621 tcatctttgt caatggacat aaattcagaa aatacgccga aagacgttta aaagactttt
    55681 aaaaaactta tttctagaga gtttttgttt cataataaga taatgtttca cgaaaggaga
    55741 tcaacttgtc cttcgaagaa aatcgaagta aaatggcaag agaaaggagc gggaagatga
    55801 acgacttatt aaaacaatac tttggttatg atgagtttag acctggtcaa aaagaaatta
    55861 tccagaaagt cattgatcag gaaaatgtat taggaatcat gcctaccgga agtgggaaat
    55921 caatctgtta ccagcttcca gctttactat tagatgggct tactgtggtt gtgtcccctt
    55981 taatctcttt gatgaaggat caggtggacg cggcgaatca attaggtata cccgccactt
    56041 ttatcaatag ctcgttagat ggttacgaaa cagctagacg cttccaagaa atcgatagac
    56101 agcagtaccg tttactttat atcgctcctg aacgttttat catgccggat tttatccaag
    56161 caatgaagcg atggaacgtc cgtatgatcg ctattgacga ggcacattgt atctctcagt
    56221 gggggcatga tttccgccca agttatttac agatggcgaa ccagctagat caactgccaa
    56281 atcgtcctgt aattgttgct ttgacagcta cagcgactgt gcaagtagct gctgacataa
    56341 aaagactttt aaaaattcca gaaaacaacc atattcagac tggttttgaa cgagaaaatc
    56401 tacgatttca agtaattaaa gatcaaaaaa aagaacaata tctaatcgaa tatttaaaga
    56461 tcaacaaaaa tcaatctggt atcatttatg cagcaacgag aaaagaagta gaccgtctct
    56521 atcatttgct taaaaaattc gatttttccg tgggtagata tcatggtggt ctaaatgaga
    56581 atgagcgaac agaaatgcaa gaagcctttt tatacgatcg cttgcagctg attgtagcga
    56641 caaatgcatt tggtatgggt ataaataaaa gcaatgtccg ttttgttatc cattatcaaa
    56701 ttcctggctc tctagaagca tactatcagg aagctggtcg ggcgggaaga gatggtcttt
    56761 ccagtgaagc catcctgctt ttctctcctc aagatatcca agtccaaaag ttttttatcc
    56821 agcagtccca aagagaagaa gggcaaaagc aaaaagaata tgaaaaatta aaagcgatga
    56881 cagaatacgt gtatattgag tcctgtcttc agcaatacat cctaaactat tttggcgaga
    56941 caagcagtcc gtgcaaccgt tgtggtaact gtttagatga cagagaaata gtagaagtaa
    57001 cgacagaagc acagatggtt ctttcttgct taaaacgtat gggtgagaac tatggtaaac
    57061 aaatgctgat gaaagtctta gcaggatcga aagaacaaaa gctgcaagct ttaggttttg
    57121 gacatttatc tacctatgga ctgatgaaaa accaatcgca aaaagaaacg atgcagttaa
    57181 ttgaatattt gatatcgaat ggctatcttc tgaccatcaa tggtgagtat cctgttttga
    57241 aagtaacaga aagagggata caagtattga aaggacaaga atctgtttat cgaaaggaac
    57301 ccaagaaagt ccagcagcta tcggatgaag aaaccgatac actatttgaa gtgctgcggg
    57361 aactgcgaac ggatctagca tcagaagcag gcgttccccc gtatgtcgta ttttcagatt
    57421 ccacattgaa agaaatgagc cgaatacgtc catcaagcag attggagatg cttcagataa
    57481 aaggagttgg ccaaagcaag cttgataaat atggcgaagc ctttttatct cggataaaaa
    57541 atgcggactc taacgttctg gataagtaga aagaacgaat acgtaaaaat gaataaaaac
    57601 aactggttaa atctgttttt tttgctcctt atgaaaatca aatatgttat aattaaggaa
    57661 ttagaatggg aggtaagcac agatggattc tttgtcgaaa aaacttgcga tccaagtatt
    57721 aaatgatgaa gcagaacgga tcaaacgatt aatccgaaat caaaacaaca gtctctgtat
    57781 ctcacaatgc aaagccttcg aagaagtggt agatacacag atgtacggct tttcccggca
    57841 agtcggtttt gctgtccgta taggagtaat cggaaagaac gagggccagc tgatgttaag
    57901 tgagctagaa cgagaattga atcagttata tagtgaggtt taccaagaaa tttatgataa
    57961 aaaagaagtt ggcaaggagg aatagacttg cctaagaaag tgaaaaaaag gcatctatta
    58021 gactatagca tcttgattcc ctatttaatt ttatgtgtat tgggattgat catggtatat
    58081 agttctacgt cgtatctatt attagaaaac ggacaaaatc cgtccgcatc agtcattaac
    58141 cagagtattt tttgggtgct tagcttgatt gctatcgcac tactttataa aatgaaaaca
    58201 gatgttttga aaaaccagcg tttgatcatg gcagccatcg cagttttgac gattttgctg
    58261 ctgattgttg tgttttttgg taaggagatc aatggggcga aaggttggct gcaaattgca
    58321 ggattctcga tccaacctgc cgaataccta aaaatcattt ctatctggta cctgtctctg
    58381 actttatcta aaagacaaaa cagtgtacaa aaagattttc ttgggactgt gaaacgtcca
    58441 cttgcaatgg ttatcggact tactgcatta gtcgctattc ttcccgattt tggtaatgcg
    58501 gcagttatct ttttgattat tttggttctg cttttagcca gcggagtcaa ttatgtgtat
    58561 actttgattg taggagtagg aggcttttgt ctaagtactt ttacgatctg gctgatcaat
    58621 atcacaaacg gaaagatctt acccggacgt ttacagtata tctataatcg atttgctatt
    58681 taccaaaatc ctttctcaga cgaattaaat aaagggcatc aactagtcaa cggctactat
    58741 gccatgttca acggaggact atttggcagg ggattaggta acagtatcca gaaaaaagga
    58801 tttttacaag aagcacaaac cgattttatt tatgcaatcg tagtggaaga actgggagtg
    58861 attatgggga ttttgatatt agcgttgcta ttctttatga ttgtgcgcat tgtccttgta
    58921 ggtatccgtt caaaagatcc gttcaattcg cttttgtgca ttgggatcgg cgcaatgttt
    58981 ttgatccaag tatttgtcaa tcttggagga attactggcg ttattccttt gacagggatc
    59041 acgttcccat ttttaagtca aggaggctcc agtttgctga tgctttcgat ttgtgtcggc
    59101 tttgtcctca acatcagtgc ggatgaaaaa cgcaagagct taggtttata ataaaaaaag
    59161 atgtaggagt ggttcgatga aaaaagtttt agttgctaac aggggtgaaa tcgctgtacg
    59221 tgtatttcgt gcttgtacag aattaggaat taaaactgtt ggaatctatg cagaagaaga
    59281 tgaatattcc gtacaccgtt tcaaagcgga tgaagcatat ttagtaggac aaggaaaaaa
    59341 accgattgat gcgtatctag atattgaagg gatcatttct atcgcgaaag aatgcggagc
    59401 tgacgctatt catccgggtt acggtctttt gtcggaaaac ttgaattttg cgcaacggtg
    59461 cgaagaagaa ggcatcattt ttgttggacc caaactgcat catttagata tttttggaga
    59521 taaaatcaaa gcaaaagctg cagctatcga agcggggatt gcttcgattc caggcacaga
    59581 tggaccaatc gcttctatag acgatgcctt ggagtttgcg aaacaatacg gttatccgat
    59641 tatgatcaag gcagctctag gcggcggcgg acgcggcatg cgtgtagctc acgatgaaaa
    59701 gagtgcaaga gagggctacg aacgtgcgaa aagcgaagca aaagcagcct ttggtagtga
    59761 tgaagtgtat gttgaaaaat acatcgctaa tcctaagcat atcgaagtac agattctcgg
    59821 ggatacgcat ggaaacgtca tccatctttt tgaaagagat tgttctgttc agcgccgtca
    59881 ccaaaaagta gtagaagtag cgccttgtgt ctctatgaat gaacagcaac gtcaaaaaat
    59941 ctgccaggca gctgtccaat tgatgaaata tgtcgggtat gtcaacgcag gtacagtaga
    60001 atttcttgtt gaaggagatg atttttactt tatcgaagtc aaccctcgcg tccaagtgga
    60061 acatacgatc acagaaatga ttacagatat tgatatcgta acaacgcaac tgttgattgc
    60121 tcaaggtttg gatctgcata aagaaatcgg attgcctcag caagaaggaa ttaaattaaa
    60181 tggttcagcc attcaatgcc gaatcactac agaagacccg ttgaataact ttttaccaga
    60241 cacagggaaa atcgatacgt atcgttcacc tggtggtttt ggcgtacgtc tggatgtcgg
    60301 aaatgcttac gcgggctatg tagtgacacc gtattttgat tcattgctag taaaagtatg
    60361 tacgcatggt gctacctttg aaacagccat tcagaaaatg gaacgttgct tgaaagaatt
    60421 tagaatccgt ggagtgaaaa caaacattcc atttatgctc aatgtcatca cacatcctga
    60481 attccaatca ggaaatgcaa aaacaacctt cattgatagt acagcaacat tatttgaatt
    60541 tcctcgacta cgagaccgcg gaaacaaaac gatgaaatat atcggtgaaa ttactgtgaa
    60601 tggttttcca ggtatcgaaa gtggcgaaaa accattttat gaagaaccac gtatgcctaa
    60661 agatctaatc acaagaactg attacgtaac ggctaagaat gtattagatg cgaagggtgc
    60721 cgatgctttg gtcgaatgga tcaaaggaca ggaaaatttg ttgttgacgg atacgacatt
    60781 tagagacgcc catcaaagtc tgctagcaac tcgtgttcgg acaaaggatt tcaagcagat
    60841 tgcacgtttg actggagaag gtcttcctga actcttttca agtgagatgt gggggggagc
    60901 aacatttgat gtggcttacc gctttttgaa tgaagatcct tggcaacggt tgagaaaaat
    60961 tcgttcttta atgcctaata cgttgctgca aatgcttttc agaggttcaa atgccgttgg
    61021 gtattctaat tatcctgaca atgtattggt ggaatttgtc aaagaagcag cagcgcaagg
    61081 aatcgatgta tttcgaatct ttgatagttt gaactggact ccacaaatgg aaaaaagtat
    61141 ccaagctgta cgggatactg gtaaaatcgc agaggcagct atttgctata ctggcgatat
    61201 caacgatcca agtcgggcaa aatacaatgt tcagtattat aaagacatgg caaaggaact
    61261 agaacaactg ggtgcgcata tcattgcaat caaagatatg gctgggctgc ttaaaccgca
    61321 ggcagcttat cgtttgatta gtgaattgaa agaaacgaca gatctgccaa tccatctgca
    61381 tactcatgat accagcggga atggaatcat cacttattct gctgcttcaa aagcaggagt
    61441 agacatcgtt gacgtggcaa tgagtgcaat gagtgggaat actagtcagc ctagcatgag
    61501 cagcttgtat tatgcactgg tcaatggtcc acggctgcca gaaatcacga ttgaaaatgc
    61561 acagaaatta aaccattatt gggaagatgt gcgcatgtat tacaaaccgt ttgaaaacgg
    61621 cttgaatgct cctgaaacag aagtatatat gcatgaaatg ccaggaggac agtattcgaa
    61681 tctacagcaa caggcaaaag ctgtcggatt aggtcatcgc tgggatgaaa tcaaacaaat
    61741 gtatcatact gtcaacttga tgtttggcga tatcgttaaa gtaactccat catctaaagt
    61801 agttggagat atggcactat ttatggtcca aaatgatctt acagaagaag atatttatga
    61861 aaaaggcgaa acactaagtt tccctgaatc tgttgtgact ttcttccaag gagaactagg
    61921 gcaacccgtt ggcggatttc ctaaaaaatt gcaaaagatt attcttaaag gcagacctgc
    61981 attgaatgaa cgtccaggtc ttttggctga atcagtcgac ttcaacgaag taaaaaaaga
    62041 attggcagaa aaaattggct atgaaccaaa acaagacgaa gtattaagct atttgatgta
    62101 tccgcaagtc tttttggact atcaaaaagc atataatcaa tttggcgatg ttacattact
    62161 ggacacacca acatttttcc aaggtatacg tttgggtgaa acaatcaatg tacaaatcga
    62221 acgcggaaaa acgcttatca ttcgtttaga tgaaatcgga gaacccgata tcgaagggaa
    62281 tcgcgtactg ttcttcaatt tgaatggaca aagacgtgaa atcactatca aagataattc
    62341 gatcatcagt acggtccaaa cccgattgaa agctgaaccg acaaaccgag aacaaattgg
    62401 agcaacgatg tctggttctg ttttggatgt attagtgaaa aaaggcgata acgttaaaaa
    62461 aggtgatacg ttgatgatta ctgaagcgat gaaaatggaa acggccatcg aagcacgttt
    62521 tgacggagaa gtcgcacatg tatatgtgtc ttcaggagac actatttcgt ctggtgacct
    62581 tttgattgaa gtgactgaga aataataagc gaaaggtggg gaaacatgaa aagactgggt
    62641 ttgttttggc tgattctgct attagttatt gtggcattct acatggaacc agtcttgttt
    62701 ccgccaaaac ttgaagtgaa ggaaaactct gatcaacaaa tatcaaacca aatgacaacg
    62761 tggaaatacc aggagcttaa agcaactggt ttttctactt atatcggaaa accgatcgat
    62821 cagctggaaa aggattttgg ccaagcctat gatcgattaa ctagcggatt tggttttgaa
    62881 acaagatatt atgaaaataa agctagtcat ctcacatttg aagcgaacat ccaagacagc
    62941 agagtcacaa ctgttaaagt actgcaaaca gggcctgctg atatcgcgcc gttttattta
    63001 gggatgacga tgcaagatct tacgaaaatc actactatct atactaattt cacttttgag
    63061 taccatgata cagaagttgg aatcgaacta atggaagagg atatgaatta tcgtccgcta
    63121 attgcttttg ataacgacac ttttgctatt ttattttttg atcaaacaac aaacggccta
    63181 ttttcagttg tgtatttaga taaagatagt cttttgaaac tattgccata tcaagtgttt
    63241 ggtgaaggcc ttccacatta tcaagcagat aaatcggctg attgggaatc aatcaatcgc
    63301 gttaaagaac gaaaaagtac gactttatta aatatgctta gaagagaaga cgaacttccc
    63361 atatacaatc aaacacttgc ctttcaaaac cacagtcagc tgctactaca tgattttttg
    63421 acaaggcctg aggaaatatt gtcagaggaa cgtatggctg attggcaaaa atcgctaggc
    63481 agtgctcaaa cgacagtgaa attctcactg acgaatgatg aattggagaa gttaatcagt
    63541 aaacaaaaat tagaaaatac tagtgggcta tttattcatc cggcgattga cgctactttt
    63601 tcgtttttat attggtacag cgatccttac aaccatgacc gtttcatgga cccgggaacg
    63661 gattctgtag gcattgcttt ttctaaagaa aatatgttag tcttattgca agaagaaaac
    63721 ggagaaagta gtgatagcag tgatcgttaa cgaagagctg tttcaactgg aggatcaatg
    63781 cctccaagtc gctgaaatga taaaaaagag taaaacgatg gcttcttatc ttgaacataa
    63841 acagtcaatg gaagaatctg caaaagtttg ccagttgaaa gaagcgttct tgtctaagaa
    63901 agaagcattt gaaaatgtag cggcatatgg tgagtatgca cctgattatc gtgaaaaaaa
    63961 acgagctgtt cgaaaagcaa aaagacagct tgatatggat gagcatgttg cagctttccg
    64021 atatagtgaa acacagctgc aaagtctgtt ggatcaaatt gtccaagtac ttgccgaaga
    64081 agtttctaat gaaatcaaag tagatgcagg tagcccgttt tttgaaacga aaaaacactt
    64141 tggatgcgga ggtaattgtc atgcaattag acaatagtga atttgaaata acaaaacgac
    64201 gcgggctgat cgtatgggtc tacagcttaa aacaattaaa gaatctgaaa aagttcggct
    64261 tgttgcatta tgtctcaaga agaatgaaat atgtcgtgct ttacttaaat gaagagacat
    64321 tggaagaaac agaagaacga atccgtaaat tacattttgt tcgaaacgta gaacgttctt
    64381 accgaccgga tatcgaaatg aattttgccg aaaaaatagg aaacaaaaca gtattaaaag
    64441 acgaaggttt cgaaattgaa gagctaaata cagaaatccg attagctgaa catctttaaa
    64501 aatctcttat gagcagtttt taaataaaaa ataacttgga taacgatcga tttttgatca
    64561 ttgtccaagt atttttttgg tatactagta ccagtaccat ttctaaggag tgagtagatg
    64621 cggataatat caggaaatta tggcgggcgc cgtttaaaaa gtttagcagg ggcgaacacc
    64681 agaccaacta cagataaagt aaaagaatcc atattcaata tgattggacc atattttgat
    64741 ggagagacag tattggatct gtttgcagga agcggtggtt tagctatcga agcagtatct
    64801 agaggctgca gtcatgcggt atgcgtagac aaaaattacc aagcgctaaa aatcatcaaa
    64861 gaaaacatcg aaattacgaa agaaccagag aaatttacca caataaaact tgatgcggac
    64921 aaagcaatcg aacggctagc agcggagaaa caacaattcg actacttgtt tcttgatccg
    64981 ccatatgcaa aacaaaaaat aatcgatcaa atccataaaa tggaagaact gggacttttt
    65041 tctaaaaccg cagttatcgt atgcgaaacg gataaaacag tcgaattgcc agaaactgtg
    65101 gctggattcc atcaaataag aaaacagatt tatggcataa cggccgtaac tatctataga
    65161 aaagagggaa atcaatgaaa cgtgcattat tcccaggaag ttttgatcct tttacaaaag
    65221 gtcacttaga tacggtggaa cgtgcggcta aattattcga tgaggtagta attggtgttt
    65281 ttatcaatac ttcaaagaaa agtttgtttc ccccagaaga aagaatgaca ttgatcacaa
    65341 aagctgtttc tcatcttcct aatgtaaaag tcatgcatca agaaaatcaa ttgacggtag
    65401 aaacagcaaa agagattggt gcagatgcgt tgatcagagg catacgaagt atcaaagact
    65461 ttgaatatga acgagagatt gctcagatga atcatcatct atatgatgaa ttggaaactg
    65521 tcttcttatt agcaaaacca gaatatagtc acgtgagttc cagtatttta aaagaagtac
    65581 tgcactttgg cggagatgtg agcagctact taccgccagt gatcaacgaa gctttagctg
    65641 gaaagcgtga atcaaatgaa agctaaacaa tggttgaaaa cagtactgat tatcatattt
    65701 ggaatgttga tcattggatt tgtagtagtt cccttgccgt attatgtaga atcaccggga
    65761 gccacgatcg atctgaaaga actaattacg gtcaatgata aggaagataa aggttctggg
    65821 tctttctctc ttacctctgt tggtatcagg caagctacag gtttcttagc tttaaaagct
    65881 aaaatgaccc cttttgaaga attgatcagt gaagaagaat tgactggtgg ggcgacaaat
    65941 gaagaatata atcaaatgca acagtattat atggattctt cccaaaatgc agcaatcgaa
    66001 caagcgttaa aacttgcaga caagccttat aaaatggaat tcaaaggcgt atatgtcatg
    66061 agtgtagaga aaacatcgaa tttctttggc aagctttcag ttggagatac agtaacaaaa
    66121 gtagacggga aaagctttca aagtacagaa gcatttatgg attatgtaaa aagccaaaaa
    66181 gttggacaaa caatcgaaat cagttatttg cataatggtg aagaaaaaac agcttctggt
    66241 gatctgatcg agttaccaac agataaaaag gcaggaatag gtatcacgct tactgaccat
    66301 accgagatcg aatcagatac agatgttcgt attaattctg gctctatcgg tgggccatca
    66361 gcgggattga tgttcacttt agaaatctat gaacaagtca ctggtaaaaa cttgcgacat
    66421 ggtaagcaaa ttgcaggaac tggaacgatc aacagtgaag gagaagttgg ccgaatcgga
    66481 ggaatcgata aaaaagtggc aagtgcggat aaagctggcg tagagatttt ctttgcacca
    66541 gatgatacga tttcagacga tgttaagaaa gaatatcctg aaataaaaac caactacgaa
    66601 gaagcaaaag ctgccgctaa aaaattgaat tcctcaatga aaatcgtacc agtaaaaaca
    66661 gtacaagaag cgcttgacta tctagagaaa atgaaatgaa actaaataat taaagagggg
    66721 ttgtgacacg agttctgtca caacccctct ttttcgtcaa aaatcttcac gataaaaata
    66781 aaaacaaaag aaaaaattga cgtaattaga tgttgaaggg acgattttgc cagagctcac
    66841 tgtttcttca attttaaatg aaaggagtaa tgaatgattt ttactggtca ttctttgaaa
    66901 aaagaacaat gatagaaaaa atcagcaaga aaatactagc ttcacccaaa ttgatcattt
    66961 ggatgtctat gatagttctt atcttttgta tcgcaagtgg ttttttatta tcaaatttgc
    67021 tattttatag agaaacagaa gagactgctg caaaagaaat actttcttcg tttagttcca
    67081 gtggagaaga tccatcagct gaaggaaacg atgtagaaga ggatgctcgt ccaaaggtca
    67141 tgtacaccga tatcaaagga agtgtgaagg agcctggtat ctattccttt tcttcagaag
    67201 aacgggtcta tgatgtacta aagcgtgcag gtggattatt agaagaagcg gatagcgatc
    67261 gaatcaactt ttctgcgaag atagaagatc agcaagtcct atatatccct gctgtaggtg
    67321 aagagctgcc agaacatctt gatcagttgg catcctcgga aggaaaacag cctacagcag
    67381 attccgagcc tcccaaaata aatatcaata cagccactcc atcagaactt cagcagattc
    67441 ctgggatcgg atcagtcaaa gcgcaagaaa tcattcgatt tagagaagaa aacggatcct
    67501 tccagaaagt ggaagacctg caggagattt ctggtatcgg tgaaaagaca gttgaaaaac
    67561 taaaaaactt cgttacaatt aaatagaatg attcaaaatc gactggcacg agcaataaat
    67621 ggacgctaat ggatactaag aataatccaa ttctaagagc ttggcaggac gaggaggtag
    67681 tatttttgga taggaaatag cttgattgtt cgcaaagaaa cattaaaaaa accaatgctg
    67741 aatgtctatc tccaaaataa aatatctggg attcatatta tgaatacggc tgtcagcggc
    67801 aataacagtc aagctctacg agaacgcttt gccaaagatg tacttagcta tacagcagat
    67861 aaagtattta ttctcattgg gacgaatgat ctagcagagc ataaacaact ttctaaagaa
    67921 acgtaccaaa aaatctgcag tggatgatag ttcagctgaa aaaagactat agttctgatc
    67981 atgctttctt tatcacgcca ccagcagtcg atgagacaaa acaaaaatat cggaataaca
    68041 agttgatcaa agaatatatc tctgttctca cagaagtagc aaaacgccat gattgtgaag
    68101 tatcgatttt tatacggaat ttcttgaaca aatggattcg aagaattttt ataatgggct
    68161 aatggatgac ggacttcatt tcggcagtga agggtaggat cttttgtcag acaggatgat
    68221 tcgttatatt taaagaatat tttgtttaag caatgagtac tttttaatca acttgaaaaa
    68281 acggaggaat ataaaacatg ttagaaaaat cttcaaaagc atgcgatacg aataaagaaa
    68341 aagttactga taagtcacat aatgaacgca ttccttggga ccagtacttt atggctcagg
    68401 cagtactgtt atctttacgt agtacgtgta cacgtttaga agtaggagcg acactcgttc
    68461 gagaaaaaag gatcattgct ggcggctata atggagcagt cagtggagat gtccactgca
    68521 tagatgaagg gtgttacgtc gtggatggac actgcttgcg aacgatccat gcagagatga
    68581 atgcactcct acagtgtgca aaacttggta tcccgacgga aggttcagaa atctatgtca
    68641 cacattttcc atgtttggcc tgtacaaagg ctttgttgca agctggtgtg aaagggatca
    68701 attatctcca tgactatcga aatgatcctt atgcccaagc gttgatcgaa caattaggtg
    68761 tatctgtaca ccaagttgct ttagactcac agcatttttc aaaacttcaa aatgaattaa
    68821 caggaaaaca tgtctagcgt tttagaaagg catgttcgaa atcggttgat ttttccggca
    68881 attacgtcga tgattatctg tttgttgtat tacactgaca attggggtta tcgtttgctt
    68941 cttgccggaa tttttttatg gttttcgcta aaagaaggac gttttatcgc gtacttgtgt
    69001 attttgtgta gcataacggt atccatttct tataggctac aagagtattc atttttagag
    69061 gaaaaaacaa catctgcgca aattactgta tttacagata cgattcgtgc aaatggtaat
    69121 tttttgacaa tggaaggaag gttatcagag aataaacaaa agctccagct ttcttatcaa
    69181 gtaaacacag aagcagaatt gaaaacatgg cttaacttcc gaggaaaggg aatagaattg
    69241 actgctgaag gcgagttcat ctctccagca aaaaagcgga atctttataa ttttgaccaa
    69301 gaagcttact tcaggaacca tagaatttct ggacggttcc aaattcaaaa gattgaacag
    69361 atcaccatgc aaaatcactg gcgaaactgg cttcagagaa aaagaggatc ttttatctcc
    69421 tttgtccaat ctgaatttcc gcaaaaaaca agtgtatata ttaattcttt attatggggc
    69481 tataaagatg caggattcag agaaccagaa gaaattttta gggagagtgg tttgctgcat
    69541 ctgttcagtc tatcgggtct acacatccaa ttttatttag ggtggctgta ttatttattt
    69601 cgacgtatcg gatggccact acacgtcagt attattccgt tgagtctact aactatctgt
    69661 tatgtttcta ttgcaggaag ttctatttct gttttgcgtg ctagtttgct gtttttattg
    69721 cgattgctag tcaaattgct gaataagcat tattccacca tggacttatt tgcagttgtc
    69781 ttttggcttc ttttactatt tgatcctcga tttctttttc atgcgggtgg acagcttagt
    69841 tttttcatga gttttttgtt tatcctgatt tcttttgatg gagattggtt gaaaaaaacg
    69901 gcctctcacg tcctgtttac tatgatcacg atgccgttga ttgtatggca tttttatgag
    69961 tggcctattt taggtagtct ttttacatta ctgattgcac cggtattcaa gtttcttttt
    70021 cttcctggtg tgttatttct cacactcttc aatcgctggc tgccggaaac tttacttttg
    70081 ctgatagaag agggattaat cttatttgag cgaataatcg atttttcttc agggtcgtct
    70141 ctcattatag gacgtttgcc ccttgtgcta tctgggatgt atattttcgg attactgatt
    70201 tgtatggata ggttgaaaga acaacctgtg aaacacttgt tttatgttgg tcttttttct
    70261 tttatttttt tgactttgcc atttttacgc ttttcttcaa cgatcgcttt tatcgatgtc
    70321 ggtcaaggag acagtatctt tttgcaaagt ccttttcgaa aagaaacgat tttgatcgat
    70381 actggaggtc agttacactt tgaaagagaa aagtgggcgc aaggtatgat acgtcctcct
    70441 gctgaaagaa atatcgttcc atatctaaaa ggtcagggga taagtgtaat cgataaactc
    70501 atcttgacac acgacgatac ggatcatgtg ggagaattac ccacgttatc tcagcatttt
    70561 actatcaaaa aaatttatat cggttggggc gctggacgaa atgagccatt acaaaggcat
    70621 ttatctgaag ctcaaaaaaa tggtactgag atcattgaaa tcaaacatgg ggacaggatc
    70681 agcggttatt atgatcttta tgtattgact ccttttgaaa agggagaggg aagaaatgaa
    70741 gattcgattg gtctatggat ggaatataac aataggcgct ttttattttt aggagatttg
    70801 aatcaagcaa tggaaaaaca attgcttttg atttatccta atctgaaagc cgatgtagtc
    70861 aaattaggtc atcacggtag ccgtacgtca agtaacacag attttctttc ccatattgaa
    70921 gcaaaacatg ggattatatc ctgcggcgta aataatcgtt atggtcaccc gcacagcgaa
    70981 gtattggaga cactagaaga aaatcagata aaaacatggc gaacggatca acaaggaatg
    71041 atcacctatc gctggcatcc agtttttcat cctcatgggc tagtggaaac aatgatagac
    71101 tagtaattct catctgctca tggtaggata atttttgaaa gtggtgagta atttgagtat
    71161 acaagcagag ttacaaaaaa tacgtaaaga aaaacttgca tcttgttact tgattttagg
    71221 aacagaaaag tttctccaag atcaagttcg gacagaaatt gtcaaaaaaa tccaattaga
    71281 gggcgaagat gacttaaatt ttttaagttt tgacatggaa aatgcaagtt tagatgaggt
    71341 agtagctgaa gcagagacgt tgccgttttt cggagatcaa cggttagtat tcgtagagaa
    71401 tccttatttt ttaacaggtg aaaaagtaaa caatggaatc gaacaaaata ctgatttatt
    71461 gacggcttat ttgaaagaac cattagaatc gactgtatta gtattctttg cgccatatga
    71521 aaagttggat gaacggaaaa aagtaacgaa acaattgaaa aaaacagcaa ttatcatcaa
    71581 tgtccaacag ttgaatgaaa aagaagtacg tcaatatctg atgaatacct tagagaattc
    71641 ctcaatcaaa atggaacgaa gtgcaattga tttattcttg aggttgaccg acttagattt
    71701 atccaaaatg atgagagagt tacagaaatt ggtcctttat ggacaaaatc aacagcaaat
    71761 cacggtaaga gaagtagaag aattagttcc taaaacgtta gaacataata tttttgacat
    71821 gacccaatat attttaaaag ggaagacgga acaagctttg cgtttatacg aagatttagt
    71881 tatgcaaggg gaagaaacaa tcaaaattaa tgcgattttg ctttctcaat tacgtttatt
    71941 tttacagaca aaatttcttg taaagattgg ttatcagcag gcaaatattg ctgaaacatt
    72001 gaaaatccat ccatatcgtg taaaacttgc catgcaggaa gtaaaaaaat tcgatgaaca
    72061 tttgctggta cagttgtttg atcaattagt tgagatggat tatcaaatca aaacgggaca
    72121 aatcgaaaaa gaattaagtt ttcaactatt cgttttgcgc acagggcagt tgttaagaaa
    72181 tcgataagaa gaaagacgag ggcaaagcat gcgggaaggt cgtttgtttt tgtccttttt
    72241 ttgacaaata aaaaaatcgg ccgaagccga ttcttttatt tagcgatttt tttgcttaaa
    72301 cgagatttgt cgcggttagc tttgtttttg tggattagac cttttgattc agccatatca
    72361 atagctttaa cagcttcttt atataacgca tctacgttgt cagcacctga agctacagca
    72421 tcttcaaatt ttttgattgc agtacgcata gcgcttgttt gagatgaatt tttcacatta
    72481 gcgttttcgc ttgtacgaac acgtttgatt gcagattcaa tgtttggcat tagtttcacc
    72541 tccgataact taagttctta cgcgtgttac cgcataattc aacattcatt attataccta
    72601 aaggcagcgt gctttgcaat atgaacatga caagtttttt tccttaacag tcttttatta
    72661 ggattttttt tcagtaaaaa cggatagtac aagctgaaaa aaatcaaaaa gacgctattg
    72721 tgggaggcaa tagcgtcaaa ccaaactaga tttttattta tctatttaaa caaagtttga
    72781 atgggaatgt ttggtactaa gttattatat aacaaagaga accgtttgca aagttttttt
    72841 tgaaaaggga tacgattccc tgttgttctt tgtttttcta ttaagaaact gcagttattt
    72901 tataaccagt atcctttgat cagtaaaggc tattctggtg taccgattat tttaatgtat
    72961 tcatcgatat tttttatcaa catttttttt atgaccaact ggttccgacg gttccatttg
    73021 ccacttactt ccagcacata tttatttagg ggcaaacgta agatatattt tgccaaatag
    73081 cgatctttaa gcaggccatt gatcacacgg tcctcaagct gcaagttgat cccagtcagc
    73141 atcattggct gttcttgtaa aacgtacgta tcaattaaaa gaccttcaaa agtttccaca
    73201 agcgtcttcc tttcttttca aagtaacctg aaaacaggca ctaattcttc cattttgata
    73261 ttcgttgaag tggatgcgat cattctttct tttgtccact gtaatctagt ataacaaaaa
    73321 agagcagaag agccaaaaag aaaaaagagc atcgaattct ataggaagtc tcattggcac
    73381 ggcctaaatt attacaaaac attgattaaa aaaagcccct gcatcgcagg agcatcccct
    73441 atatcgtttt aataggtcaa tcaaaaagca gtatcaaaca cattttgtat caaatcagga
    73501 aataatagat aagctaagcc cagaatcaaa agtaccgcta gaagaaacca gccgattgtt
    73561 cctatgaaat actttaattt ttcgaacatt gtatcacctc gttgtcagta tatcacggat
    73621 cagtaaattg ggggtgttaa gaatctccta aaatagacaa aatttgttct attttcctaa
    73681 agcaaatact tgtaagatac agtaagtgat accagaagca atgaccggtc cggcggcgat
    73741 tcctcttaag aagacaacac ctaaaatcgt gccgaaaaca agtgcaacag tgatttgtgg
    73801 atcagaagca atcaatccta cacccttgga agataagaca gccactagcc caccgcaaaa
    73861 aacagctacc cagccaatag gggatttaaa ggcttctaat agatcacgta gaccgatttc
    73921 tcctgttgcg atagggatta gaatagccgc tgtgatgatc gtcaccccaa tattgattcc
    73981 tttgccttga agccaaggga aaaaggaacg ggaggctggt atgagcttca taatcaaaac
    74041 aaaagctgcc gctatcatca gactttgatt tttagctaaa attgctacaa gtaaaataat
    74101 tcctaaaaaa agccaacttt ccatgaaata tattcctcac tttcttcgaa acaatcataa
    74161 catgttttta gaaagttaca aaagtaggaa ttcatccttc aagacctatt cataagctgt
    74221 ttttttcata aaataaacaa aatcataaaa aaacactttg cttatttgac agaagacagc
    74281 cgcgctttgt atagtggaca agacttacat ttttttgaag aatcagaaag atcaggtaaa
    74341 ggtcttagta aacgcaagcc attcagaata accaatattg tgcttccttc gtgtcctaca
    74401 actccaagag gtagatttaa aaattgcagg aagttagaag caatcaacaa gagaataaca
    74461 agaatcgaaa aaataatatt ttgtttgatg atccgccgca tttttaaaga tagccgatga
    74521 ctgtagacaa atttatttaa gtcgcttttc attaagacga tgtctgcgat atccatagcg
    74581 gcatctgtcc cttgccccat agcgacacct aaagaagcgg ctgctaaagc aggcgcatca
    74641 ttgactccat caccaaccat cgcatttact tggtaaagtt cattctcttt tttgactagt
    74701 tgtgtttttt cttccggagt acagcttgca tagtaatgtt caatccctac ttgttcagca
    74761 atggcctttg ctgtgccaga atggtcgccg gttatcatac ttgtatggat gttttgggat
    74821 ttaaagtagg aaattacttg ttgggtattt gcttttggaa tatctagcag acctaaaaca
    74881 gcaattaact gctgatcttt agaaagataa atcacggttt tgccttgatt ttccaaacgt
    74941 tcgatcattt ccgcaatctc tttgctgatt atcatcgtct caggatcata tgcgtgcttg
    75001 ccaactttcc attgcgcacc taaataggta gtttgtaatc caaaaccagt aatttcttct
    75061 attggtaagt attggatttc ttcaggtatt gtgcaatcga aatattgcaa aatcgcatga
    75121 gcaagtggat gcgtcgtttt ttgttccata gcaacaagaa gttgttgtgc caaatccttt
    75181 tcatcaaaga aaaagctgtc agttacaact ggttttcctt tagtgagcgt tcctgtttta
    75241 tcaaaggcaa tcgcttttaa ttcagaaagc ttttctaaat ggataccgcc tttgattaaa
    75301 atcccatttt ttgcagaatt tgaaagagca gctagagaag ctggtgtagc agatgcgaca
    75361 agcgcacaag gagaagccac aactaataat accattcctc gataaaagct ttcatcccaa
    75421 ctccaaccta ggaaaaagtg ggggagaagg atcattagag gaacagcaag caaaatcaat
    75481 tttacataat tattttcgat tcgttcgata aatgaagctg tttgagttgg cgtattctgc
    75541 gcttcttcta ctaattggat aatcttagca aagacagttt cggaactcgt ttttgttact
    75601 cgaatcgtga tgggatttcc ttgattcagc gttcctgcat acatctcatc gcctaaatat
    75661 ttttctacag gcacagattc accgcttaat gcagactcat cgatcgttgc ctgtgtgctt
    75721 tctaaaaagc catctatcgg aacagctgct cctttagcga caaataccaa gttatccacc
    75781 tttagttgag aaacatctac ttcttgcatt tgtccatttt cttttaataa ttgggctttt
    75841 tgtggttgaa gattcatcaa agcagtaatt tctttttgac ttttattagt tgtgtattcc
    75901 tctaatgcac cgcttaaaca aaagatgaat gtcaacattg ctccttcaaa gtaattaccg
    75961 atcaaacagg caccaatagc agctaaagcc atcaataaat ccacattcag tgtccgatca
    76021 ttccacaatt ctttcaagcc ttccgatgtt tgtttatatc caccgatgat aatggcactg
    76081 atataaaaaa gtggggtgat ggtaagagag aatcggtcga agataaaacc gaagatcata
    76141 aataaaaggc agccgattgt tgctgcaaaa gcttttttct gattagtcat cgttattaga
    76201 cctccttgtc aaaacaagga tagcataata aaaatgtaat tgataataat aacagctaaa
    76261 tgagaacgat aatggttatc atttggtgat taagcttgat gttgttttta ttatttataa
    76321 tgattattat taaagaaaat aaaaaaccag aacatttctc aaataaga
//
LOCUS       DAZULO010000004        74001 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000004 DAZULO010000000
VERSION     DAZULO010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 74001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.004"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..562
                     /locus_tag="ACG8OA_000248"
     CDS             <1..562
                     /locus_tag="ACG8OA_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFS4975096.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            762..1076
                     /locus_tag="ACG8OA_000249"
     CDS             762..1076
                     /locus_tag="ACG8OA_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4975097.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1097..1474
                     /locus_tag="ACG8OA_000250"
     CDS             1097..1474
                     /locus_tag="ACG8OA_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4975098.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1484..2257
                     /locus_tag="ACG8OA_000251"
     CDS             1484..2257
                     /locus_tag="ACG8OA_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975099.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2279..3682
                     /locus_tag="ACG8OA_000252"
     CDS             2279..3682
                     /locus_tag="ACG8OA_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS4975100.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3864..5048
                     /gene="mobT"
                     /locus_tag="ACG8OA_000253"
     CDS             3864..5048
                     /gene="mobT"
                     /locus_tag="ACG8OA_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS4975101.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5045..5335
                     /locus_tag="ACG8OA_000254"
     CDS             5045..5335
                     /locus_tag="ACG8OA_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975102.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5332..5553
                     /locus_tag="ACG8OA_000255"
     CDS             5332..5553
                     /locus_tag="ACG8OA_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975103.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5595..6374
                     /locus_tag="ACG8OA_000256"
     CDS             5595..6374
                     /locus_tag="ACG8OA_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFS4975104.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6435..6935
                     /locus_tag="ACG8OA_000257"
     CDS             6435..6935
                     /locus_tag="ACG8OA_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS4975105.1"
                     /translation="MEMKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEE
                     YAIHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENK
                     EDIHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHG
                     VFEYCQ"
     gene            6991..7629
                     /locus_tag="ACG8OA_000258"
     CDS             6991..7629
                     /locus_tag="ACG8OA_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFS4975106.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7701..8096
                     /locus_tag="ACG8OA_000259"
     CDS             7701..8096
                     /locus_tag="ACG8OA_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4975107.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8080..10533
                     /locus_tag="ACG8OA_000260"
     CDS             8080..10533
                     /locus_tag="ACG8OA_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4975108.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10530..12557
                     /locus_tag="ACG8OA_000261"
     CDS             10530..12557
                     /locus_tag="ACG8OA_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS4975109.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12554..13576
                     /locus_tag="ACG8OA_000262"
     CDS             12554..13576
                     /locus_tag="ACG8OA_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFS4975110.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13593..14522
                     /locus_tag="ACG8OA_000263"
     CDS             13593..14522
                     /locus_tag="ACG8OA_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4975111.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14836..14883
                     /locus_tag="ACG8OA_000264"
                     /pseudo
     CDS             14836..14883
                     /locus_tag="ACG8OA_000264"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14899..15699
                     /locus_tag="ACG8OA_000265"
                     /pseudo
     CDS             14899..15699
                     /locus_tag="ACG8OA_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            15937..16173
                     /locus_tag="ACG8OA_000266"
     CDS             15937..16173
                     /locus_tag="ACG8OA_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975112.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            16180..18132
                     /locus_tag="ACG8OA_000267"
     CDS             16180..18132
                     /locus_tag="ACG8OA_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFS4975113.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(18204..18701)
                     /gene="dfrG"
                     /locus_tag="ACG8OA_000268"
     CDS             complement(18204..18701)
                     /gene="dfrG"
                     /locus_tag="ACG8OA_000268"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFS4975114.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            18976..20103
                     /locus_tag="ACG8OA_000269"
                     /pseudo
     CDS             18976..20103
                     /locus_tag="ACG8OA_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            20201..20389
                     /locus_tag="ACG8OA_000270"
     CDS             20201..20389
                     /locus_tag="ACG8OA_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFS4975115.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(20447..20800)
                     /locus_tag="ACG8OA_000271"
     CDS             complement(20447..20800)
                     /locus_tag="ACG8OA_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4975116.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            21331..21753
                     /locus_tag="ACG8OA_000272"
     CDS             21331..21753
                     /locus_tag="ACG8OA_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4975117.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            21750..21980
                     /locus_tag="ACG8OA_000273"
     CDS             21750..21980
                     /locus_tag="ACG8OA_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4975118.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            22478..22678
                     /locus_tag="ACG8OA_000274"
     CDS             22478..22678
                     /locus_tag="ACG8OA_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFS4975119.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            22705..23898
                     /locus_tag="ACG8OA_000275"
     CDS             22705..23898
                     /locus_tag="ACG8OA_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4975120.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(23966..25531)
                     /gene="guaA"
                     /locus_tag="ACG8OA_000276"
     CDS             complement(23966..25531)
                     /gene="guaA"
                     /locus_tag="ACG8OA_000276"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS4975121.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(25590..26513)
                     /gene="coaA"
                     /locus_tag="ACG8OA_000277"
     CDS             complement(25590..26513)
                     /gene="coaA"
                     /locus_tag="ACG8OA_000277"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFS4975122.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            26709..27314
                     /locus_tag="ACG8OA_000278"
     CDS             26709..27314
                     /locus_tag="ACG8OA_000278"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4975123.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            27622..28638
                     /locus_tag="ACG8OA_000279"
     CDS             27622..28638
                     /locus_tag="ACG8OA_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4975124.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            28779..30080
                     /locus_tag="ACG8OA_000280"
     CDS             28779..30080
                     /locus_tag="ACG8OA_000280"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFS4975125.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            30126..30788
                     /gene="deoC"
                     /locus_tag="ACG8OA_000281"
     CDS             30126..30788
                     /gene="deoC"
                     /locus_tag="ACG8OA_000281"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFS4975126.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            30809..31207
                     /locus_tag="ACG8OA_000282"
     CDS             30809..31207
                     /locus_tag="ACG8OA_000282"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFS4975127.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            31273..32355
                     /locus_tag="ACG8OA_000283"
     CDS             31273..32355
                     /locus_tag="ACG8OA_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4975128.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            32567..34129
                     /locus_tag="ACG8OA_000284"
     CDS             32567..34129
                     /locus_tag="ACG8OA_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4975129.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            34122..35255
                     /locus_tag="ACG8OA_000285"
     CDS             34122..35255
                     /locus_tag="ACG8OA_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4975130.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            35255..36211
                     /locus_tag="ACG8OA_000286"
     CDS             35255..36211
                     /locus_tag="ACG8OA_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4975131.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            36310..37476
                     /gene="deoB"
                     /locus_tag="ACG8OA_000287"
     CDS             36310..37476
                     /gene="deoB"
                     /locus_tag="ACG8OA_000287"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFS4975132.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            37500..38318
                     /locus_tag="ACG8OA_000288"
     CDS             37500..38318
                     /locus_tag="ACG8OA_000288"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS4975133.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            38335..39042
                     /gene="deoD"
                     /locus_tag="ACG8OA_000289"
     CDS             38335..39042
                     /gene="deoD"
                     /locus_tag="ACG8OA_000289"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS4975134.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(39340..40026)
                     /gene="gpmA"
                     /locus_tag="ACG8OA_000290"
     CDS             complement(39340..40026)
                     /gene="gpmA"
                     /locus_tag="ACG8OA_000290"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS4975135.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(40198..40779)
                     /locus_tag="ACG8OA_000291"
     CDS             complement(40198..40779)
                     /locus_tag="ACG8OA_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4975136.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(40912..41595)
                     /gene="rpiA"
                     /locus_tag="ACG8OA_000292"
     CDS             complement(40912..41595)
                     /gene="rpiA"
                     /locus_tag="ACG8OA_000292"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFS4975137.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            41914..42327
                     /gene="rpsL"
                     /locus_tag="ACG8OA_000293"
     CDS             41914..42327
                     /gene="rpsL"
                     /locus_tag="ACG8OA_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFS4975138.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            42411..42881
                     /gene="rpsG"
                     /locus_tag="ACG8OA_000294"
     CDS             42411..42881
                     /gene="rpsG"
                     /locus_tag="ACG8OA_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFS4975139.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            42975..45062
                     /gene="fusA"
                     /locus_tag="ACG8OA_000295"
     CDS             42975..45062
                     /gene="fusA"
                     /locus_tag="ACG8OA_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFS4975140.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            45234..46421
                     /gene="tuf"
                     /locus_tag="ACG8OA_000296"
     CDS             45234..46421
                     /gene="tuf"
                     /locus_tag="ACG8OA_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS4975141.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            46828..47136
                     /gene="rpsJ"
                     /locus_tag="ACG8OA_000297"
     CDS             46828..47136
                     /gene="rpsJ"
                     /locus_tag="ACG8OA_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFS4975142.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            47167..47796
                     /gene="rplC"
                     /locus_tag="ACG8OA_000298"
     CDS             47167..47796
                     /gene="rplC"
                     /locus_tag="ACG8OA_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFS4975143.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            47824..48447
                     /gene="rplD"
                     /locus_tag="ACG8OA_000299"
     CDS             47824..48447
                     /gene="rplD"
                     /locus_tag="ACG8OA_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFS4975144.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            48447..48737
                     /gene="rplW"
                     /locus_tag="ACG8OA_000300"
     CDS             48447..48737
                     /gene="rplW"
                     /locus_tag="ACG8OA_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFS4975145.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            48776..49609
                     /gene="rplB"
                     /locus_tag="ACG8OA_000301"
     CDS             48776..49609
                     /gene="rplB"
                     /locus_tag="ACG8OA_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFS4975146.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            49652..49930
                     /gene="rpsS"
                     /locus_tag="ACG8OA_000302"
     CDS             49652..49930
                     /gene="rpsS"
                     /locus_tag="ACG8OA_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFS4975147.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            49953..50300
                     /gene="rplV"
                     /locus_tag="ACG8OA_000303"
     CDS             49953..50300
                     /gene="rplV"
                     /locus_tag="ACG8OA_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFS4975148.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            50314..50970
                     /gene="rpsC"
                     /locus_tag="ACG8OA_000304"
     CDS             50314..50970
                     /gene="rpsC"
                     /locus_tag="ACG8OA_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFS4975149.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            50973..51407
                     /gene="rplP"
                     /locus_tag="ACG8OA_000305"
     CDS             50973..51407
                     /gene="rplP"
                     /locus_tag="ACG8OA_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFS4975150.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            51397..51585
                     /gene="rpmC"
                     /locus_tag="ACG8OA_000306"
     CDS             51397..51585
                     /gene="rpmC"
                     /locus_tag="ACG8OA_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFS4975151.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            51610..51876
                     /gene="rpsQ"
                     /locus_tag="ACG8OA_000307"
     CDS             51610..51876
                     /gene="rpsQ"
                     /locus_tag="ACG8OA_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFS4975152.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            51934..52302
                     /gene="rplN"
                     /locus_tag="ACG8OA_000308"
     CDS             51934..52302
                     /gene="rplN"
                     /locus_tag="ACG8OA_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFS4975153.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            52339..52647
                     /gene="rplX"
                     /locus_tag="ACG8OA_000309"
     CDS             52339..52647
                     /gene="rplX"
                     /locus_tag="ACG8OA_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFS4975154.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            52675..53214
                     /gene="rplE"
                     /locus_tag="ACG8OA_000310"
     CDS             52675..53214
                     /gene="rplE"
                     /locus_tag="ACG8OA_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFS4975155.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            53233..53418
                     /locus_tag="ACG8OA_000311"
     CDS             53233..53418
                     /locus_tag="ACG8OA_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFS4975156.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            53454..53852
                     /gene="rpsH"
                     /locus_tag="ACG8OA_000312"
     CDS             53454..53852
                     /gene="rpsH"
                     /locus_tag="ACG8OA_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFS4975157.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            53884..54420
                     /gene="rplF"
                     /locus_tag="ACG8OA_000313"
     CDS             53884..54420
                     /gene="rplF"
                     /locus_tag="ACG8OA_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFS4975158.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            54581..54937
                     /gene="rplR"
                     /locus_tag="ACG8OA_000314"
     CDS             54581..54937
                     /gene="rplR"
                     /locus_tag="ACG8OA_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFS4975159.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            54958..55458
                     /gene="rpsE"
                     /locus_tag="ACG8OA_000315"
     CDS             54958..55458
                     /gene="rpsE"
                     /locus_tag="ACG8OA_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFS4975160.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            55473..55652
                     /gene="rpmD"
                     /locus_tag="ACG8OA_000316"
     CDS             55473..55652
                     /gene="rpmD"
                     /locus_tag="ACG8OA_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFS4975161.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            55696..56136
                     /gene="rplO"
                     /locus_tag="ACG8OA_000317"
     CDS             55696..56136
                     /gene="rplO"
                     /locus_tag="ACG8OA_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFS4975162.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            56136..57431
                     /gene="secY"
                     /locus_tag="ACG8OA_000318"
     CDS             56136..57431
                     /gene="secY"
                     /locus_tag="ACG8OA_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFS4975163.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            57493..58140
                     /locus_tag="ACG8OA_000319"
     CDS             57493..58140
                     /locus_tag="ACG8OA_000319"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFS4975164.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            58331..58549
                     /gene="infA"
                     /locus_tag="ACG8OA_000320"
     CDS             58331..58549
                     /gene="infA"
                     /locus_tag="ACG8OA_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFS4975165.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            58582..58698
                     /gene="rpmJ"
                     /locus_tag="ACG8OA_000321"
     CDS             58582..58698
                     /gene="rpmJ"
                     /locus_tag="ACG8OA_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFS4975166.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            58716..59081
                     /gene="rpsM"
                     /locus_tag="ACG8OA_000322"
     CDS             58716..59081
                     /gene="rpsM"
                     /locus_tag="ACG8OA_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFS4975167.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            59109..59498
                     /gene="rpsK"
                     /locus_tag="ACG8OA_000323"
     CDS             59109..59498
                     /gene="rpsK"
                     /locus_tag="ACG8OA_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFS4975168.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            59578..60516
                     /locus_tag="ACG8OA_000324"
     CDS             59578..60516
                     /locus_tag="ACG8OA_000324"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFS4975169.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            60544..60924
                     /gene="rplQ"
                     /locus_tag="ACG8OA_000325"
     CDS             60544..60924
                     /gene="rplQ"
                     /locus_tag="ACG8OA_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFS4975170.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            61196..62035
                     /locus_tag="ACG8OA_000326"
     CDS             61196..62035
                     /locus_tag="ACG8OA_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4975171.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            62011..62880
                     /locus_tag="ACG8OA_000327"
     CDS             62011..62880
                     /locus_tag="ACG8OA_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4975172.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            62870..63667
                     /locus_tag="ACG8OA_000328"
     CDS             62870..63667
                     /locus_tag="ACG8OA_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFS4975173.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            63870..64616
                     /gene="truA"
                     /locus_tag="ACG8OA_000329"
     CDS             63870..64616
                     /gene="truA"
                     /locus_tag="ACG8OA_000329"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS4975174.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(64949..67618)
                     /locus_tag="ACG8OA_000330"
     CDS             complement(64949..67618)
                     /locus_tag="ACG8OA_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFS4975175.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(67856..68044)
                     /gene="rpmB"
                     /locus_tag="ACG8OA_000331"
     CDS             complement(67856..68044)
                     /gene="rpmB"
                     /locus_tag="ACG8OA_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFS4975176.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            68314..68676
                     /locus_tag="ACG8OA_000332"
     CDS             68314..68676
                     /locus_tag="ACG8OA_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4975177.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            68728..70410
                     /locus_tag="ACG8OA_000333"
     CDS             68728..70410
                     /locus_tag="ACG8OA_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFS4975178.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            70527..72563
                     /gene="recG"
                     /locus_tag="ACG8OA_000334"
     CDS             70527..72563
                     /gene="recG"
                     /locus_tag="ACG8OA_000334"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFS4975179.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            72612..73613
                     /gene="plsX"
                     /locus_tag="ACG8OA_000335"
     CDS             72612..73613
                     /gene="plsX"
                     /locus_tag="ACG8OA_000335"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFS4975180.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            73735..73977
                     /gene="acpP"
                     /locus_tag="ACG8OA_000336"
     CDS             73735..73977
                     /gene="acpP"
                     /locus_tag="ACG8OA_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS4975181.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT        24348 a        13160 c        15503 g        20990 t
ORIGIN      
        1 attaaaaaat gaatttggaa tagaagttaa agattatttt attgacttag atttattaca
       61 gctaataaac agtgtgaaaa gaatgtatag tggagacgac aacggtgttg atttatacaa
      121 atttgttacc aaacatatat ttgttaatct taatattgac gagaaaaatt acccgtctat
      181 ttttaagcac tatctatttt ttattaacga agtagaagat cggataaaaa ataattcatt
      241 atctagtagt gtatctagtt ttaagaagat gtttaaatct cgagatggta tcacacttac
      301 tacagcacat aaagttaaag gaaatgagta tgatactgtt atcgctatta atcttctaaa
      361 cgggaaaatt cctcattgga atgaaatttt taatgtttct gataaaggaa ttagttcagc
      421 gagaaaacta ctatacgtaa tttgttcaag agcaaaaaat aatttatttc ttttctcaga
      481 ggtgggttat tttacgagta aaggagactt gctttatccc actcaattat tagataaaat
      541 agaatttgat tataatattt aaaataccta ggtagtcgtt gttttaaata tagtaggaat
      601 cggacgctca gtacggagcg tctatttttt tacccaattt tgaaaggaag tgaacttatg
      661 aaaataaaaa atcaagaatc aaaaggtcgc tctcccccct ttaaaaccat cacacaaata
      721 agattcattc aaacataaaa acagaaagga taggtaaaaa tatggaactt aaatatgtca
      781 ttcccaatat ggaaaaaaca tttggacaat tagaatatgc tggtgaaggg aacattgaac
      841 aacgtcgaat aaatggcaga ccaaccattc tctctcgtag ttacaattta tactcaacga
      901 ttcaacgagc agatgatatt gttgtcattc ttccctccga agctggcgag aaacattttg
      961 aatccgacga aaaagtcaaa ctcatcaacc cacatattac cgcagaaggt tataaaattg
     1021 gcaatcgagg atttaccaat tacattatgc acgcagacga tatggtgaaa gcataagaaa
     1081 ggaaaggtga aaaaatatga gattagcaga aggtattgtg attgaaaaag accagacatt
     1141 tggcacattg aaattttccg ccttgcgtcg tgaggttcgt atccagaatg aagatggtac
     1201 ggtttccgaa gaaattaaag aacgtaccta tgacttaaaa tcaaaaggac aaggacgcat
     1261 gatacaagtc agtatccccg cttctgttcc tttgaaagaa tttgactaca acgcagaagt
     1321 ggaattaatc aatcctgtgg cagataccgt agcgacggct acttttcgag gggcagacgt
     1381 tgattggtac gtgaaagcag acgatcttgt attaaaagga cagacaaaac ctcatcagaa
     1441 atataaaaaa gaagcgtccg aaagtgaaaa ataggtgttg cttatgacaa acacaccatt
     1501 gattttgaaa gaaattccga aggatgaagc catttctttt attcggcaat accattattc
     1561 aaagatactc cctagattgt gtaaatactt tctagggatt ttttcagaag aaaaattatt
     1621 aggcgttgtg gaattaggct ggggtacgca acccctacaa accatacgca aactctttcc
     1681 cgacagttcc cttcaaacca cagattattt agaaattgga aagatgtgtt ttcttccaga
     1741 aatgaatcaa accaattatt ttggcagtca agctctttca gctctaatta agtggttgaa
     1801 agaacatacg gactgtcatt ttttatatac actggcagat ggaattgagg gaaaatgtgg
     1861 ctatgtctat caagcgtcca acttctttta ctgcgggtat ttcaagacaa gtgtctatcg
     1921 tgataagcag tcatgggaaa agatacaccc tcgaagtgct cgccttttat tagaggaaaa
     1981 tgctcgcttt gaacaggtag agaaaaagca ttggctttcc caggcatttt gcgaatacaa
     2041 agggattgag aaaatcaatg gacgcatgtt tcgctatctc tatccgttga caaaagaagc
     2101 gaaaaaactg ttaggacaca ctctctatcg acgtcattat tatccgaaag aaaaaaattt
     2161 acgctttgaa aagcgaatcg cttatcagaa atatgaagcc atttctcaac caacttttga
     2221 taaacaggct cgtatctaca atacacaatt attttgatag gtggaaagga ggatttttat
     2281 gccattgctt catgacagag gagagcgaat caaagccaac gataagcact tggtctatca
     2341 ttttgtacta atttcattgc tggtagtttt tctagtcgtc ctactccctt ttcattggag
     2401 agcgttacaa tcggtgaact ggcaagcagt tcagttagat acatttttct atcaacttca
     2461 tacgccatac ttttggagca gtattctcac tgctcttttt gtatgcggac ttatcagctg
     2521 gctttattat cactatcgta tagattctat aaaacagtta gagcaccgtc aaaagttagc
     2581 tcgtatgatt ttagaaaatc attggtatga agccgaagaa actcaatcag agggattctt
     2641 taaagattta cccagcaaga aaaccaaaca acgaatccgc cattttccta aaatatatta
     2701 tcgaatgaaa gatggactaa tccatattca tgtagaaatc accttaggga aatatcaaga
     2761 tcagcttttg cacctagaaa acaaactaga aacaggattg tattgtgaat tagtggaaaa
     2821 aacgttgaaa gattcttatg tagagtatgt cctactttat gatactattg gcaatcgaat
     2881 tggcattgaa gacgtggtag ctcaagatgg acgtgtcaaa ttgatggaat cggtctattg
     2941 ggagttcgat tctctccctc acatgttaat tgctgggggc actggtagtg gaaaatccta
     3001 ttttattctc acattgattg aatctctcct gcatacaaat gcaaagcttt atattcttga
     3061 ccctaaaaat gctgatttgg cagacttagg ggcggtcatg aataatgtct attaccgtaa
     3121 agaagatatg ttggcatgta tcaatcaatt ttacgaagat atgattgatc gcagtgaaac
     3181 catgaaacaa caccctaact ataaaacagg agaaaattat gcttatcttg gtttgtctgc
     3241 caattttctt atttttgatg aatatgtggc aatgatggat atgctaggtc gtgaaagtac
     3301 caccgttatt cataaactca aacaaattgt aatgttagga cgacaagcag gcttcttttt
     3361 aattcttgcc tgtcaacgtc cagacgcaaa atattttagt gatgggattc gggacaactt
     3421 taacttccgt gtggctttgg gtcgtatgtc tgaaatgggc tttggtatga tgttcggaag
     3481 cgatacacaa aaacaatttt tcttaaaacc catcaaggga agaggttacg tggatacagg
     3541 aaaaagtgtg attagtgagt tttatacacc ccttgtacca aaaaggtacg atttcttagg
     3601 agaaatagga aaagtcattc aaaaaaagca atctgagcct gtaccgcacg aagtgaaagg
     3661 tacaggttca gacttgcttt gattggtgtg gcgttagcca cgccaatcct ttagccccct
     3721 ctatctaaca ggggggcaca aatacaactg aaaacaggca attctcatca accaatgttg
     3781 atggtacaaa ggattgcgga aaatatgaaa ggtgtaaact caagggtcat agtttacatc
     3841 ttttttagat tggaggtttg cgaatggaaa acgaaaaaat ttggattaag gaattgaaag
     3901 aaaagcgact ggcttatggc gtttctcaaa ataaactggc ggttgcttct catattacca
     3961 gaccttacct atcggatatt gaaacaggca aggcagtccc tactcaacag gtgaaagaag
     4021 atttactgaa cgctttggaa cgcttcaatc ccgataaccc attggagatg ttattcgact
     4081 atgtacggat tcgattccct accaatgatg tggctcaaat tatcggtgag gttttacgtt
     4141 tgaatatgga ttatatgcta cacgaagact ttggctatta ttcttatccc gaacactatc
     4201 gtttcggtga tattgttgtc ctagtctctc atgatgtaag taaaggtgtt ttattagaac
     4261 tgaaaggaaa aggctgtcgg caatttgaaa actttctatt ggctcaacat cggagctggt
     4321 atgatttctt tcaagattgc atggaacata aaggtatttt caaacgcttg gatttagcta
     4381 tcaacgataa aacagggatt ttgaatattc cagatttggc aaagaaatgt aaacaggaag
     4441 aatgcatttc cgtctttcga agctttaaaa attatcgcag tggtgaattg gttcaccgtg
     4501 atgaaaaacc atacatggga aacacacttt atattggctc attaaaaagt gaagtctatt
     4561 tctgtatata tgagaaagat tatgagcaat acgtgaaaaa tgatgttccc ttagaagacg
     4621 cagaagtaaa gaatcggttt gaaattcggt taaaaaatga ccgagccacc caagccatga
     4681 aagatttatt ggcacatcaa caggcggaaa aaacagcctt tgaaattatc aatcgctata
     4741 tccgctttgc agataaagac gacactaaac gtcggagtga ttggaaaact aatgaacgtt
     4801 gggaatggtt tatcggtaaa aatcgtggag cattacgcct aaccacacag ccagagcctt
     4861 attcttttga acgaacattg aactggttac atcatcaagt cgcccctaca ttaaaaatcg
     4921 caagcatttt agatgttctc aacggtacaa ccattatctc caccatgatt caggaagcca
     4981 aactgacaga aaaacatgag aaattaattg aacaacaaca tctagccatg gaggatttga
     5041 ttacatgagc cttttaacaa aagaattaaa gaagttaggg tttcagtgtg gcattgagtt
     5101 tcaagcgtat atacaaaata cagggaaata tacgtctctc attattgagg gaaaacggca
     5161 agctggtgat accatctata cctatgattt ttacaaggtg agattttata ataattatac
     5221 caatcgagta accgtctatg gagagcattt aacccctttt caattattaa ggcgtgtaaa
     5281 aagctatatc tattatcgag aaaaatatct taaggaaagg aggacaatca catgaatttt
     5341 gggcagaatt tgtacaactg gttcttggat aatgcacagt cattggtatt gttagcgatt
     5401 gttgtcattg gactgttttt aggttttaaa cgagaatttt caaaactcat tggcttcttg
     5461 attattgctc taatcgccgt cggacttgtg ttcaatgcag ctggcgtgaa agatgtgtta
     5521 ttaaacttat ttaaccgaat cattggtgct taacagatat ttttcatgat aaaataggaa
     5581 gtatatattt tgttatgaaa ggatttatga atatgtttac aaatgatcaa tttaaatctc
     5641 ttttaattaa aacattagaa agaaagagct cttcaaatta ttatgaagga ggtaacgaga
     5701 ttgtttctaa aatgaaaata tcagaggtaa ctttagatga gactgacgat tttacttatt
     5761 ataaaggata taaaaagggc tggaatacgc tttgcacata tcttaaaata agagttcctt
     5821 ttgatgattt agactttttt gaaagtcata aagatttgat tacccagaca gcaagctcca
     5881 tatatgataa acaaggtgat aatgtattag tggatactat tttagtacca cttccagaga
     5941 attacgaagt aattaacttt agtcagttga aaattagtga tgtggtttca caagctattg
     6001 aagacgctga gtcttttatg agtaatggtg aatatcaacg tgcctttgac agagttcaca
     6061 ctgcttttca tggatattta attgaaatac taaaaaaata tgaaattaca gttcctagag
     6121 acgaaaatct atctaaatta tatagtagga tacaacaatt aattgaaaaa gaaattcaac
     6181 ctactgaact tgctgatata gtgaagacaa cgataaggtc atcaaatgga atgattagct
     6241 ctcttaatga agcaagaaat aggcattcac ttgcacaccc taatacaaat attattggaa
     6301 agagagaagc aaaactaata ataggtattt caagtacagt tacagattat attagcggat
     6361 accttgataa gtagcgtcat taactgattg ctactttttt tatgactatt tttataaaaa
     6421 ggatgtgata agaattggaa atgaaagtct atgtggcaaa tttaggacgg tataatgaag
     6481 gtgaattagt cggagcatgg tttactccac caattgatga ggaagaaatg gcagaaagaa
     6541 tcggtttaaa tgaggactac gaagaatatg caatacatga ttttgagctt ccttttgatg
     6601 tggacgaata cacgccgatt agtgaaatca accgtctatg tgaagccatt caagaaatcg
     6661 aaggtacacc tatttataat gaactcaaag aaattcaagg catgtggttt agtagcttgg
     6721 aagaattact ggaaaacaaa gaggatattc attgttatag tgattgtgat tcgatggaag
     6781 atgtcgcccg ttattacgtg gaagaaacag gacagcttgg tgaagtccct tccaatttac
     6841 aaaattatat tgactaccaa tctttgggac gtgatatgga aatagagggt aattacctag
     6901 ttacctctca tggggtattt gaatattgtc agtaagttta acaatccgtg ttataattaa
     6961 attaataaat agcacggaga ggtataaatt atggtaaatg tatttgaaaa tcttaaacta
     7021 ttaaaactag acatgaaaga taaaggctgg gtgatagatt ccttttactt tcgttacaaa
     7081 cagcaaaatt atattgtact tgttaagcta tttgaaaaag aggaaaaagt tccagaatat
     7141 gctctgttaa aactagaatt tttaaaggaa aacgattttt cagatatgct agctgtctat
     7201 gctaattcgg taaaattata tacagacacg aaaacaataa gagaatattt tggtatcgaa
     7261 tacagtagta acttgggaga tgttttattt caatttagcc aaactcttgc tcgatttatt
     7321 ccgacagaag ttagtgagaa gaaaaatgaa gaccaaaaag aagccatgtg tttctcatta
     7381 agtcaaagtg attcagaaga tcccagaaaa aaatattgtt tttcagttag aagaaatcct
     7441 ttaaagggaa atggtgagct gggaaaacga agtcctttta atgacaataa aacgagattg
     7501 tatagaccca gcttatatga acgattaggg tcagatacca atttaagttt cagatattca
     7561 atgaatccag atgatgaaga aactgatgaa ggtattattg caaaatggac aaaaaataaa
     7621 attgaatagt taaagcattg aaagagcaat cacctatttt gattgttctt tttctatttt
     7681 tacggaaagg acgtgaagac atgaaaaaaa taagaagtta caccagtatt tggtcagtag
     7741 aaaaagtgat ttatgcgatt aatgatttac aactcccttt cccaattacc tttacgcaaa
     7801 tgacatggtt tatcgtttct ttgtttgtcg tcatactctt tggcagttta ccaccacttt
     7861 ctctgattga cggagcacta ctcaaatact tagggattcc cattgttctc actgggttca
     7921 tgtgccaaaa gacctttgac ggaaagaaac cctatggatt tctaaagtct atcattacct
     7981 atgctctacg ccctaaagta acctacgctg gcaaacaaat acgattaaaa aaagaaacaa
     8041 tgaatgaatc gattacgatt gtaaggagtg aacaactaga tggcatatcc gattaaatat
     8101 atggaagata atctcgtatt taatcatgat ggcgaagtat tcgcttatta tgaattaatt
     8161 ccctataatt acagtttttt aagttccgat gaaaaaattc aagttcatga taatttctgt
     8221 caattgattg cccaaaatcg tgatggcaag attcatgctc tgcaactagc aaccgaatca
     8281 agtattcgag ccacacagga acaatcgaaa aaagaaatca ctggacgatt aaaagagata
     8341 gcttacgaaa aaatagatga acagaccgaa gcgttaattt ctatgattgg tgacaatcaa
     8401 gtagactatc gcttttttat tggttttaaa ctctacctca atgaacagga agtatccgta
     8461 aaaaatgttg ggaaagaaat tcaaaaagca tttgcggaat tctttcatgg tgtgaatcac
     8521 aaattaatgg gcgattttgt ttctatgcct atggcggaaa tcgaacgctt ttctaagatg
     8581 gaacagttgt tagaaagtaa attatccaga cgctttcaga ttcgtaaatt ggagaaagat
     8641 gattttggtt atattattga acatctttac gggcaaacag gtgtcccttt tgatgactac
     8701 gactaccatt taccgaaacg atacgaagat aaaaaagtgt tggtaaagaa atacgatatt
     8761 ctcaaaccca ctcgctgttt aatcgaagaa aatcaacgct atttacggat tgaacaggaa
     8821 gataccacta cttatgtggc gtattttaca attaatagta ttgttggtga attggatttc
     8881 cctaatagtg aaatttttta ttaccagcaa cagcagttta ctttccccgt tgataccagt
     8941 atgaatgttg aaattgtcgc caataaaaaa gccttatcta ccgttcgcaa taaaaagaaa
     9001 gaactgaaag atttagatag ccacgcatgg gaaagcgaca acgaaaccag tactaatgtg
     9061 gtggacgctt tagaatctgt cgatgaatta gaaagtaatt tagaccaaac caaagaatct
     9121 atgtacaaac taagctatgt ggtacgggta tcagcaaaga atttggacga gctgaaacaa
     9181 cgttgtgatg aagtaaaaga tttttatgat gatctgtcta tcaaattagt acgacctttt
     9241 ggcgatatgc taggcttgca taatgaattt attccctcaa gcaagcgata tatcaatgac
     9301 tatattcaat atgtcacgtc ggattttttg gctggtcttg gtttcggtgc tacacaaatg
     9361 attggtgaaa attctggtat ttacgtcggt tacaatctcg atacaggcag aaatatttat
     9421 ctccaaccta gccttgcaag tcaaggaatt aaaggctctg taacaaatgc attagcgtcc
     9481 gcttttattg gttcattagg tggcggaaaa tcattctcta acaatatgct ggtctattat
     9541 tcagtactct ttggcggaca ggcaaccatt gttgacccaa aagctgaacg tggtgaatgg
     9601 aaagaaacct tacctgatat tgcccatgaa atcaatattg ttaatctcac gagtgagaac
     9661 gacaataaag ggttacttga cccttatgtg attttaaaac tcccgaaaga ttcagaaagc
     9721 cttgccattg atattctcac gttcttaaca ggtatttcca gccgagatag cgagaagttt
     9781 cctgtcctgc gtaaagctat tcggagtgta actcaaagca aacaacgagg gttgttattg
     9841 gtgattgatg aattgcgaaa agaagatacg cccatcagca atagtattgc tgaccatatc
     9901 gaaagctttg tcgattatga ttttgcccat ctgttattca gtgatggcag tgtcacccaa
     9961 tccattagct tggaaaaaca actcaatatt attcaagtgg cagatttggt cttacccgat
    10021 tccaatagct cttttgaaga atacactacc atggaattat taagtgtggc aatgctgatt
    10081 gtcattagta cctttgcttt agactttatc cactctgacc gttccatttt taaaattgtt
    10141 gaccttgatg aggcttggag ttttttacaa gtggcacaag gaaaagctct atctatgcga
    10201 ttggttcgag caggacgtgc catgaatgca ggggtctatt tcgttactca aaacgcggac
    10261 gatttactag atgaaaagat gaagaataat attggcttga aatttgcctt tcgttccact
    10321 gacctagtgg aaatcaaaaa gaccttagaa ttttttggtg tggacaagga agatgaaaac
    10381 aatcagaaac gcttacgtga attagaaaat ggacaatgtt tagtttcaga catctatggt
    10441 cgtgtagggg ttatgcagtt tcacccgatt tttgaagagt tgcttcatgc ctttgatacc
    10501 agaccgcctg tgcgaaatga ggtggaagaa tgacacaaaa acaaaagaaa tttcttcact
    10561 atagttggat aacgctcctt tgtgtgggcg ttattttact tttattaggc acgctgggca
    10621 atgcagtaca agcgacaggc ttagttgatg aaaccattga tacaagcaat gaatactcca
    10681 agtatggttt gaatcaatat cagcttgatt attatgtgga caatagctgg ggctggcttc
    10741 catggaattg gagtgatggc ataggacaat cggtgatgta tggattgtat gccattacta
    10801 attttatttg gacaatttct ctttatctat cgaacgccac aggttatttg gtgaaagaag
    10861 cctatgcctt agatttcatt agtcaaacat cccaagcaat tggcgaaaac atgcagacga
    10921 ttgcaggcat tacaccgaat ggcttttcca gttcagggtt ttatgtcggt ttcctccttc
    10981 tttttattct cattgttgga atctatgttg cctatacagg attggttaag cgagaaacga
    11041 cgaaagtgat acgagctgtt ttaaactttg tcgtggtgtt tattctatcg gcttccttta
    11101 ttgcgtatgc acccgattat attgcgaaaa tcaatgattt ttccaaagat gtcagtaccg
    11161 ccagtctgaa tattgggaca aaaattgttc ttcctaacac ggatagtcaa gggaaagaca
    11221 gtgtggacat gatacgtgac agcttattct ctgttcaagt aaagcaacca tggctattgc
    11281 ttcaatatgg ctccactgat gtagaagcgt taggggaaga acgtgtggaa agtttattgt
    11341 ctaccagtcc agatacaaac aatggtgaag atagagaaaa tgtagtgatt gaagaaattg
    11401 aagatcatga caatacctat ttaaccctac cgaagacgat ttccagatta ggaacagtct
    11461 tctttttgtt tgtattcaat attggtatct cgatttttgt tttccttcta acaggaatca
    11521 tgattttttc gcaagtgcta tttattattt tcgctatgtt tttgccaatc agtttcctat
    11581 taagtatgtt gccgagtttt gatggcatgg gcaggcatgc cattaccaag ttatttaatg
    11641 tgattatgac aagggctggt attaccttga ttattaccgt tgcctttagt atttccacta
    11701 tgctttattc gctgtcagca agttctccct ttttcatgat tatgttttta caaattgtga
    11761 cctttgcagg gatttatttt aaactgggcg atttaatgag cctattcact ttacaaagca
    11821 acgactctca aaacatgggc agacaagtgt ttcgcagacc tcgtcaaatg atgaatcgac
    11881 aaaccagaag atttcaacgc agtatgagta agatttttcg tggaaaagca gggtcaacga
    11941 agcaaaagac aatgacaaca aagcagaatc aaaccaataa agctaaccat actcgtcaaa
    12001 atgaacggac tacaccaaag aaagaaacag cactttccaa tgtgaaaaag caacaaggcg
    12061 gtatcgggca acgattaggt gaaaagacag cccatgtatt ggacacgaaa aatcgtctag
    12121 tcaataaaag aaagcaggca aaacaacaga tacaggcgac accaacccat gtcaaatatg
    12181 cccttcatca aggaaaagag aatgtcactc ataatgtaac agactttaaa caatctatgg
    12241 cagataccaa agctcaacgc aaacaacagc gtatgaacca gcaggagcaa aaacgaaaga
    12301 acattgctca aaagcgtttg gagctggata aagcaaaaga aaaaagacga aaagaaaacc
    12361 catcatctcc tgttcagaat aaccagcagc atgttcgcaa gcgtcagcaa accgtcacgc
    12421 ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg accattgaaa aaagagcaaa
    12481 aacaaaagac accaccgaag caattcaatc agaggaagct aaataaaaga acggataagc
    12541 gaggacaaca aacatgaagc taaaaataac agtgatttgt agtgtggtct tctctctctt
    12601 cttttttctc ttattattcc tagtcatttt ctttgcagat gatacggaca gtggcgaaaa
    12661 caacaaggat tcttctattc cacaaggcgg tgtgaccgtt tcaccagaag tgttggcaca
    12721 ccgtccgttg attgaaaaat atggtaagga atatgggatt gaagactatg tttcttatat
    12781 cttagccatt atgcaagtgg aatcgggcgg tacagcagaa gatgttatgc aaagttccga
    12841 atctttgggt ttaccgccca acagtttgag taccgaagag tccatcaaac aaggggttaa
    12901 atatttcagt gaattattaa ccagtgcaga acaacaagga gtggatatag attctgttat
    12961 ccaatcttat aattatggcg gtggcttttt aaattatgtg aggagtcatg ggaaaaaata
    13021 tacctatgaa ttagccgaac agttttctaa agagaagtcg ggtggtcaaa aagcagacta
    13081 tcccaatcct atagccatac ctgtgaatgg tggttggcga tataactatg gaaatcagtt
    13141 ttatgttcag ttggtttcac aatacctaac cgacacttcc ccaacagaat ttgatgatga
    13201 aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa gggtttccct atgtctttgg
    13261 aggggcgtcg cctaccacct cttttgattg tagtggctta atacaatggg tgtatgacaa
    13321 agctggcatt tctctccctc gtgtcgccca agatcagtat gacgctacgc aagaaatctc
    13381 tatggaagaa gcccaagcag gcgaccttat cttctttcat tcaacctata atgcaggcac
    13441 atatgtgact catgtggcga tatatttaga gggaaatcgc ttttatcatg caggcgaccc
    13501 aatcggttat ggcgacttaa gcagtcgtta ttggcaagac catctgattg gggctagacg
    13561 tgttattcat aactaagaaa ggaatgaatc aaatgattat aaaaatcgag cgaaaagaaa
    13621 agaaggagaa accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta
    13681 aagttggcac gcataagaaa ttaacctttg ttttatgggt tttattgatt ggtagtgtcg
    13741 gatttggaat ctataaaaat tttactgcca ttgacactca tactgtccgt gaaacagaaa
    13801 tcattaaaca gcagattgtc gataccaacc aagtagaaag ttttgtgaaa tcctttgcga
    13861 aagactactt ttcgtggcaa caatcccaag aagccattga taaacgaaat gaaaaattga
    13921 cccattattt gacggaagaa ttgcaggtat taaatgaaga aatgattcgt aaggatattc
    13981 ctactagttc tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg aatgagaaca
    14041 cctttgaggt actgttttct gttgaacaag ttattaccga agacaaagac aaggaaacta
    14101 tatcgtctag ctttcatgtg gttgtccata tagatgaatc agataatatg gttattatca
    14161 aaaatccgac aatgagtaag aaacctcaaa aatcggacta ccaacccaaa cagctagaaa
    14221 gtgaccatac agtagataca gaaacgatgg acgaaatcac tagcttttta gaaacgttct
    14281 tccagctcta tcctaccgcc acagaaaaag aattgactta ctatgtaagt aatcatgtgt
    14341 tacctatgat taacaaggaa tatgttttcg aggaattggt gaatcctatc tttaccagaa
    14401 aagataacca agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa
    14461 cccaaatctc gcaatttgag ttgatattag aaaaacaaga taattggaag attgtaaaat
    14521 aacaaatatt ggtacatgat tacagatact ttgtaatcat gtactctttt tgataaaaaa
    14581 ttggagattc ctttacaaat atgctcttac gtgctattat ttaagtgact atttaaaagg
    14641 agttaataaa tatgcggcaa ggtattctta aataaactgt caatttgata gcgggaacaa
    14701 ataattagat gtcctttttt aggagggctt agttttttgt acccagttta agaatacctt
    14761 tatcatgtga ttctaaagta tccagagaat atctgtatgc tttgtatacc tatggttatg
    14821 cataaaaatc ccagtgataa aagtatttat cactgggatt tttatgccct tttgggtttt
    14881 tgaatggagg aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgca
    14941 ggaaaaacta ccttaacaga aagcttatta tataacagtg gagcgattac agaattagga
    15001 agcgtggaca aaggtacaac gaggacggat aatacgcttt tagaacgtca gagaggaatt
    15061 acaattcaga caggaataac ctcttttcag tgggaaaata cgaaggtgaa catcatagac
    15121 acgccaggac atatggattt cttagcagaa gtatatcgtt cattatcagt tttagatggg
    15181 gcaattctac tgatttctgc aaaagatggc gtacaagcac aaactcgtat attatttcat
    15241 gcacttagga aaatggggat tcccacaatc ttttttatca ataagattga ccaaaatgga
    15301 attgatttat caacggttta tcaggatatt aaagagaaac tttctgccga aattgtaatc
    15361 aaacagaagg tagaactgta tcctaatatg tgtgtgacga actttaccga atctgaacaa
    15421 tgggatacgg taatagaggg aaacgatgac cttttagaga aatatatgtc cggtaaatca
    15481 ttagaagcat tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc
    15541 cctctttatc atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt
    15601 actaataaat tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc
    15661 aaaattgaat atacaaaaaa aagacaacgt cttgcatatt aagtagttca gttttggagt
    15721 acaaaaagtg aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt
    15781 tttttattgt taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt
    15841 atataaaaat gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg
    15901 ttacaattta ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat
    15961 aagaacgcta agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa
    16021 tacgctgaat atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt
    16081 ttagacgata agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt
    16141 gaaaaaatgg gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta
    16201 gccacgaagg aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa
    16261 gatagggcta aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa
    16321 atgttgttct tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat
    16381 tactgcatca atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta
    16441 aaagggaaac caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt
    16501 atgtactgca atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct
    16561 ttatctaatt ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa
    16621 gatgttgagc cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca
    16681 ttcaatgagc cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat
    16741 caatgtaagg cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact
    16801 tatcaccaac aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt
    16861 tcagtcagtc gtacttgtga tattttagga attggagttg gaacctacta tcataagctg
    16921 gagtggctgt ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa
    16981 acaaagaaat ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac
    17041 gtccgtaaga aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa
    17101 acgtatatag ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc
    17161 tatgattgga atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac
    17221 catctcaata cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa
    17281 ccatccgcta acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact
    17341 aataggagta gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac
    17401 tattggttaa ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac
    17461 ttctctatta gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca
    17521 catcatttta tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc
    17581 ggacaagcaa aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt
    17641 ctacggactt tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa
    17701 gaggctatta gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat
    17761 ccattagcca ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct
    17821 ctggaaccaa acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc
    17881 tttattcaac agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt
    17941 ggtgataaga agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt
    18001 acaattctaa gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt
    18061 ttgacacctg ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt
    18121 tatttcaaat gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg
    18181 ttcttcttca attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa
    18241 tagtagttat acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat
    18301 tcctcataat tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg
    18361 atgtacattt tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata
    18421 aaaatttctt cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca
    18481 caaccattaa aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag
    18541 gctcttccga ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta
    18601 tttttaacat attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc
    18661 actctattct tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta
    18721 actaatttac catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg
    18781 ttagttgaat aagcaatttt ttctataagt tatattatca taataccaca aaatcacaaa
    18841 ttttcattca attatcattt tcaattgcat tttataaatc attttgtatg taatttggta
    18901 tgtaagttaa aatgaaaaaa gtagtccatt ttgaagtaca ttttgtcatt ttgtactcca
    18961 aaactgaact acttacttgc atatatacgc ctttatagtg gagtactaca tttacgagat
    19021 tcggttagag tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat
    19081 ggtgaattat gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag
    19141 tttttgaagt taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt
    19201 gaaaatccgc accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa
    19261 atgttgcttg atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg
    19321 gattctacga cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt
    19381 agtgcactgt tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt
    19441 tatatggaga gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat
    19501 cctttctggg cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag
    19561 tatgagagct cggtttctct tggatactta aatcaatcat ttcaaaatgc agttatggaa
    19621 gggatacgct atggttgcga acaaggatta tatggttgga atgtgacgga ttgtaaaatc
    19681 tgttttaagt acggtttata ctatagccct gttagtactc cagcagattt tcggatgctt
    19741 actcctattg tactggagca agcctttaga aaagctggaa cagaattgtt agagccatat
    19801 cttagtttta aagtttatgc accacaggaa tatctttcac gggcatataa cgatgctccc
    19861 aaatattgtg caaatatcgt aaatactcaa ctgaaaaata atgaggtcat tattattgga
    19921 gaaattcctg ctcgatgtat tcaagattat cgcaatgatt taactttttt tacaaatggg
    19981 cttagtgttt gtttagcaga gctaaaagga tatcaggtta ccactggcga acctgtttgc
    20041 cagacccgtc gtctaaatag tcggatagat aaagtaagat atatgttcaa taaaataact
    20101 tagtgcgttt tatgttgtta tataaatatg gtttcttatt aaataagatg aaatattctt
    20161 taatatagat ttgaattaaa gtggaaagga ggagattgtt attataaact acaagtggat
    20221 attgtgtcct atttgtggaa ataaaacaag actacgaata cgagtggata ctatacttaa
    20281 aaatttccct ttatactgcc ccaaatgtaa gaacgaaact ttaattaatg ttcaaaaaat
    20341 gaatataata acaatcaaag agccagacgc caagacgcag agccgataat ttgagaaatg
    20401 aaactctcat cttatcggct ctttttgttt atctgaattt tactgactag ccttcaatat
    20461 ttcttgcttc attgatacct tttgctagtg cttccattaa ggatagttct ttgtctgtaa
    20521 agctatccat gtatttttct acctgtaacc ttcgagtgct tttaatcaaa ttatctgtag
    20581 gtaagaaaaa ttcatcaata gatacatgga gtaatgatac aagatcatac agcacttgtg
    20641 tacttgggtg ttgtcctttg ttttctatat ttgttaaata acgtgggtca atttcaatca
    20701 ttgttcccac ttgttcacga gtgagacccc gtttcattcg agcctcttta atcgctaaac
    20761 caacggctct aaaatcgtat ttatcttctt ttttacgcat actaaatcac ctctatacat
    20821 tttactgttc ctatataacc ataaacaggt atagaaaaac atgttatata gtttatgagt
    20881 tcctatagaa cactgtggaa tttattaatg cgatgaatcc aatttcaaat agaaaggtcg
    20941 ctgattttat ttacctatac aactttataa aatttagtca tactcctaga aagtagataa
    21001 aattggcgac ttaatggagg gaaaagtcgc catgagaaag ataatctgtc atctagttct
    21061 cttaattaag ttcttatcaa aaaaacttat ccacctgtac tggatagata tgaccatcaa
    21121 tgtggttttg cagattacat agatagaaaa atcaaagcat atgaacagag aaaacaatct
    21181 gtttatgtgc ttttttggtc acttaataaa tatcttttag gatttctctt catttatgca
    21241 acaaatcccc cgttcgtgtc ggggtttagt atgaagggag ataaagaagc acatgccact
    21301 ccctttcacg acagaaaggg ggtgaaaagc atgaaaccat cttcttttca agaaaccata
    21361 gaaaatcagt ttgactatat ctgcaaacaa gttattgaag atgaacgaaa agattacttc
    21421 aaacatctag acagactatc agcaaaagaa gtctcgtttt ctgatataga taactacctt
    21481 gtcaactctt tttctactgt tgatacgtac gtcactgatt tccaactatt taaattattt
    21541 ggattaagaa tcaatgttga aagtgattta ttaagtgaag ccctgcgtca tctttcagag
    21601 aaaaaacgga acattatttt acttcattac tttatggata tgagtgatgt ggagattgcg
    21661 gagttactaa atttgaatcg ttcgactgtt tatcgtcatc gaataagtgg actagccatg
    21721 attaaagaat tcatgaagga gtgtaaagaa tgaaaacagc ttatccaagg gttcccttcc
    21781 ccctcattgt aaaggcgaca gatggcgacg tggaagcaat taatcagatt gttaaacatt
    21841 atcgagggta tacctcaaaa cgttcattac gtcgcatgac agacgaatat ggaaattctc
    21901 atatggttat tgatgaaacc ctacgtggca gaatggaaac gaaactcatc tctaagattt
    21961 tatcgttcga aattaaatag tcccattttt tcgtggaagc gtgtcgaatc gtatccacgc
    22021 ttcccgattt gggaggtttg ttattccatc aaagcatatt gtgtgttcag taggttttga
    22081 taggctaaca agccaaacgt tctttgacaa ccaaataaag gcaatcgaat acgtttcggt
    22141 aagaaaagag ctgatgaact aacacgccat gacctacaaa attagtgagc gattcatgtt
    22201 agtaattcga aagtaatctt tagcaggatt gctgccatga ctttcttatc gtgataatga
    22261 tactcccgta tagccatagt ccgagcgtta gaagcgtcac aggcaatggg tacggctaca
    22321 tgagaaccat gcagaagtga aattcctgtg agctttgcta gagctgttcg attgctaaga
    22381 tattcttctc ttgtcagtgt attattataa aactatttga tataattata ggtgaatatt
    22441 ggagttttcc tagcctataa ttgggaaagg agtaataatg ataacggtca caaaaaagga
    22501 tttagttgag ctaggatatg gaacttcttt ttcagcagac atcataaaaa aagcaaaaga
    22561 attgatgatt acaaaagggc atatctacta tcaatctcga aagctagata gagtccctcg
    22621 tgaagctgtt gaagaaattc taggaattcg gttttcaaat gaaaacaggt ctgagtagtt
    22681 tttgcacagt aaggagtaaa aactatggtt aaagacccaa taaaaaaagc aaagaatgga
    22741 acctattatt ttcgtgcaaa tctaggttat gattctaaag ggaaaaaaat tcaaaaatat
    22801 cgtagtggat tcactactaa aaaagaagca agagaagaat attcgaaact gctattgatg
    22861 aaaccagaag aattaagtga gaataaggat aaaatgacgt ttaaacatta tatctttgaa
    22921 attttccttc catggtataa gacacaggta aagctcagaa catatgaaaa tcgcttacca
    22981 actattaaga agcatttctc ttattttgat aaaatggctg tttccgatat tgagcctatt
    23041 gatgtgcaaa attggcaact aaaattatca aagaagtgta agtcttctta tgtaagagct
    23101 gttcaaggac tattttcagt tgctatggat cgagcaattg tccttgggat aaccaccaca
    23161 aatccatcta agattattgg caatgtgaaa aagcaaaagt ctaaaattga attttggaca
    23221 aaagaagaat ttgaaaaagt aatctctcta atttacaaag aagattacta tcaacacttt
    23281 ttatttatct ctctgtggtt tttatttatg actggaatgc gaattggtga agctactgcc
    23341 attcaatggg aagatattga ttttgactca ggagtactaa ccatcaacaa aacactctat
    23401 tacaagaatc aatcgaatta tagatttgtt gaaccaaaaa caaaggcgag cgttcgtcat
    23461 attgtattag ataaatgcac attaacttat ttatccgaat ggaaggaagt acaacaaaat
    23521 ctaattcaga cagactttgt aatgagctat aacggtattc ctacccaaaa acatacacta
    23581 tcatatgcta ttgaacgcta tgctaagctg gctggtatcc atcgaatcag aatccatggg
    23641 ttacggcatt ctcatgcttc cttgttaatc agtatgggag aaaatccact tatcattaaa
    23701 gatcgtctag gacacgaaga tattgagacg actttaggaa cgtatggaca tctttatcct
    23761 aacagtaatt tcgaggtagc taacaaatta aatggagcca tttcctttaa agaagcaaaa
    23821 caaaattatg atacttcacc aaagaatcaa tttactgtag actacttgcg aaacaaagaa
    23881 atgaaaaaag tgcaataaaa gtgcagtaaa aagataagaa gagccagaaa cccttggtat
    23941 aagaggattt ctagctctcc tacaactatt cccactccac tgttgctggt ggtttagaag
    24001 tgatgtcata cacgatacgg ttgacgtggg ctacttcgtt tacgatacgt acagagattt
    24061 tttgcaagac ttcccatggg atacgggcaa agtcagcggt cattccgtcg attgaagtta
    24121 cagcacggat accgactgtg tagtcgtaag tacgtccgtc acccataaca ccgacagagc
    24181 ggatccctgg aagaacagtg aaatattgcc agatatcacg gtcaagtcca gcagctgcga
    24241 tttcttcacg caagatagca tcagattcgc gtacgatttc tagcttgtct tcagtgattt
    24301 cgccaagaac acgaatacca agaccaggac cagggaatgg ctgacgccaa acgattgaat
    24361 caggcatgcc taactgagtc cctaattcac ggacttcgtc tttgaataat gtgtttaatg
    24421 gttcgatcaa ttcaaattgc atatcttctg gcagtccacc tacattatgg tgagatttga
    24481 tcgtctgtgc tgtttctgta ccagattcga taacgtcagt atacaacgtt ccttgtgcta
    24541 ggaaagagac gccttcttca ccagcaagtt ttgttgcttc gtcatcaaaa acatatacga
    24601 attcattccc aatgatttta cgtttttgtt ctgggtcaga tacacctttc aacttgtcta
    24661 agaagcgatc tcttgcatcg actttgatga tgttcaagcc gaattttccg cctagacttt
    24721 ccattacttg ttccgcttcg cctttgcgta gcagaccgtg gtcaacaaag atagatgtca
    24781 attggtcacc gatcgctttt tgtaaaagaa ccccaacgac actagaatca acgccacctg
    24841 aaagaccaag caagactttt ttgtcaccaa cttgttcacg gattttttct acttgcatat
    24901 cgataaagtt atccattgtc cagttacctt cacagtgaca aacatcaaag gcaaagtggc
    24961 gcagcaattc gattccgtat tcggaatgac gtacttctgc atggaattgg ataccataaa
    25021 aattacgtgt tgtgtcttgg atagaagcaa tcggacaatc tttgcttgtt ccgactgttt
    25081 caaagccttc aggaacttta gttaccaagt caccatggct catccatact gtttgttgtt
    25141 ttggtgtttc tttaaacaag acagcttgat cgcttgttac ttctaacaag gctttcccat
    25201 attcgcggtt tttagcaggc tctacggaac cgccaagttt gtacgtgatc aattgcatac
    25261 catagcaaat ccctaataca ggaataccta aattgaagat ctcaggatcg atcccaaatg
    25321 cattctcatc atatacactg tttggtccgc cagaaagaat gattcccttt ggattcatcg
    25381 cttttacttc ttctgcagta atgcggtggc tcaataattc ggaaaagact ccaagatcac
    25441 gaatacgtct tgtgatcaat tggttatact gactaccgta gtctaatacg ataatctttt
    25501 caacgttcgt catatcggca acgtttgtca aatttattac ccctatccct ttaaatttta
    25561 tctcaaaata gtcaaactat tttaaaatgc taatattttc gcatgaaaat cgaatcgatg
    25621 atatgatttt cagttttatg gagaattata tcggctcttc ctcgtgtagg caagatatat
    25681 tcctgtaaat ttttcaaatt cacatttttc catacatctt ttgccatttt aaaggcttct
    25741 tcacgatctc cgatagcaaa acgataataa taattctctg gattttggaa agccgtatcc
    25801 agtaacgcac caaaacgatc caaataccat ttttcgatca gcacgggatc ggcatcgaca
    25861 tagacagaaa aatcaaaaaa gtcgctgacg tagatttgct gattcgcagg taattgaagc
    25921 gtattgatcc cctcaacgat caagatatca ggctgctgga tcaattcgta cttgtctgga
    25981 atgatatcat acacatcatg tgagtaaaca ggtactttta tctcttcctg accagatttt
    26041 acacggttca agaaatcgat cagcatctcc atatcataac tttcaggaaa tcctttacgg
    26101 tccatgattc cacgctcttt taatatacga tttggataaa gaaatccgtc agtcgtgatc
    26161 agctgaacgt ttcttcgttt gaaggtacga gacaaaatcg tctgcagcag tctggctgtt
    26221 gtactttttc caacggctac acttccggcg atcccgataa taaaaggcgg tacttctacg
    26281 tatttatgaa gaaataaacc tttactcaag aacaaagatt caaattcttt catataaaga
    26341 taaatcagat gagcaagagg tacatagata tctcgaacat cctgcatgga aatgcggtca
    26401 ttcaaacttt tgatattatc tagttcatct tgtgtcaaag gtgcttgccc attacgataa
    26461 aattctcgcc attcttctct tgccacacgg tagtagttca ttggatcatt catattagcc
    26521 tcctgcgttc tcccatcaat ggaactattt taacacaaaa taaacgcaaa ccccaccact
    26581 taagagagat tgccgatctt tttgtataat ctttataata gaaagaaacg gagtcttatt
    26641 cttgacttgc gcacggatac ataaaaggat agactgttgt catgaaagaa atgagaagga
    26701 tggaagacat gacaaatcat tactattctg aacgcccaga tacggcgcac gattttgaac
    26761 aatgggcttt tgagttaaaa ggcagaacct ttcaatttgt aacagattct ggtgtatttt
    26821 cacgtgaaac agtggattat ggttcacgtg tcctgattga tgctttcgat tgggaagagt
    26881 tacctgatga aggaaagatc ttagatgtgg gatgcgggta tggaccgatt gggttagctt
    26941 tggcatttgc gacacagcgg tttgttgaaa tggtcgacat taatgaacga gcggtaagtc
    27001 tagcgcaagg aaacgcaaag cgtaaccaaa tcgaacatgt tgatatccat caatcgaata
    27061 tctatgaagc agttcatgaa gaaacctatg cagctatcgt aagcaatccg ccgatccgtg
    27121 ctggaaaaaa agttgtacat gaaattttga caggcgcata tcctcggttg aagaagggcg
    27181 gtacactgac aattgtgatc cagaagaaac aaggagcgcc gagtgcgcaa aagaaaatgg
    27241 aagaaacatt tggtaatgca gaaatcgtca caaaagataa aggatattat atcatcaaaa
    27301 gtgtgaaaga ataactagct gaaaaatagt ggagcgacga aagagtggaa ctgggcgtgc
    27361 atgtacgggt acgtatcagt tctttttttg ttgagaagtt tttaaaaaca aagaactatt
    27421 ttttcttcaa aaagaaatag taggaacaga gtcgttgttt tttacgataa aataaataag
    27481 atacagaact tgtatagaag gctgccaagt cggtagataa aggaggtttt tatcaaaaaa
    27541 catactttcc ttttttctct attcttttct tgggggtttt ggtacactga tagggaattt
    27601 agatagaagg gagccaatag gatgaagctg accatcagag atatcgcaga aatggcaggc
    27661 gtatcagtaa caactgtctc acagattttg aataacaaag gaagcagatt tagtgataag
    27721 acaagaaaaa aagtgttgtc gatcgtgaat gaacatcatt ataagcccga ttatttcgct
    27781 tcaaatgtga tcaatcgaca ttcaaagacc atcggaatga tcgtccctga tgtcactgat
    27841 ttcttcttct ccaaactgat cgaaggtgta gagagctatt tgaatccttt gggctatgtg
    27901 atcatgttat gcaattctcg tcatagccaa gaaaatgaga tcaagtattt acaagagctt
    27961 tcccaccgct cagtggacgg aatcttgctg gccacaccaa atattttacc tgagaaatat
    28021 gcattagaca gcggttttta tcaaaaaatg ccaatcattt taatcgatcg cggattgaat
    28081 cgtcgggata acggacggct tatcgtaaag gaatatgagg gcgcctatca agctgtttcg
    28141 ctgtttattg aaaatggcca tacgaagatc ggcatgctga aggaaaccac tggatactat
    28201 caattagaag aacgtttcaa cggatatcgt catgcgctga aagatcatgg cctgcctttt
    28261 aatgggaaat ttgttgaaca tggagagttg actgtccaag gtggatatac tgcatcaaaa
    28321 aaattactca aacataaaga tatcacggct attttttgcg ggaatgatgc catggcgatc
    28381 ggatgctatc aggcgatcga tgaattgggc aaaaaaatcc cagaagatat ctcagtcata
    28441 ggattcgatg gattaaagct gtcagaatac atgattccgc aattgacgac agtgcagcag
    28501 ccaagttttg atattggatt ttatgctgca cgtttcctga tcgatacgat cgaatttcct
    28561 caaagaaaag ttccgaacaa agtgttcgaa acaaaattaa ttattcgtga gagcgtaaaa
    28621 gtattgacaa agggctaact cggcggtata ttgcttatgt aagcatttac aaaggctagt
    28681 tagtgctttt tttattttta gtaaaccagt ttactaaagt ggtttacctt tcctaaaaat
    28741 aatgtataat accgaatgaa gacaaggagg atcaggcaat gcgaatggtg gatctgattg
    28801 aaaaaaagag ggatggaaac gagctgtcga aagaggaaat cgagtacatc gtcacgaact
    28861 atacgaatgg aaagattcca gattatcaag tcagtgcatt attgatggca attttttatc
    28921 aagacatgac aaatgaagaa atcacgaact taacattggc gattgctaat tctggtgatg
    28981 tcatcgattt gagttctctt gaaggaataa aggtagataa gcattcgaca ggtggcgttg
    29041 gtgacacgac cacattgatt ttagcccccc tagtagctag tgtcggagtg acagtggcta
    29101 aaatgtctgg acgcggtcta ggttacacag gtggtacact agacaagcta gaagcaattc
    29161 ccggatttca gatcgagctt tcggatgaag aatttgtacg cattgttaat gaaagtaaag
    29221 tagcggtaat tggtcaatca ggaaacttgg caccggcgga caaaaaattg tatgccttgc
    29281 gtgacgtcac agcgaccgtt gattctcttc cgctgattgc cagttcgatt atgagtaaaa
    29341 aaatcgcggc aggagcagat gcaatcgttt tggatgtgac cactggagac ggtgctttca
    29401 tgaagaacat cgaagatgca agacgtctgg caaagaccat gacgagcatt gggaaactgg
    29461 ctaaccgaga aacagtggcg gttatctcag atatgtcaga acctcttggc gaagctatcg
    29521 gaaatagtct agaagttgtc gaggcaatcg aaacattaca aggaaatggt ccagaagatc
    29581 tagtagaaat gtgctacgcc ttaggaagcc aaatggttgt tcttgctggc aaagctaaga
    29641 caaccgatga agcaagaacg ttgcttcaag aagctctgga atcaggtaaa gcgttagcaa
    29701 aattcaaaga aatgatccaa aatcaagggg gagacccagc gatcgtggaa catccagaac
    29761 gaattttgac tgcacgctac acaatggagc ttcctgctaa acaaagcgga gttgtgtcaa
    29821 aaatcgtggc aaatgaatta ggaattgctg caatgatgct aggtgctggg cgtaaaacca
    29881 aagaagaaga tattgatcac gctgttggat tgaaactgca taaaaaaatt ggtgacactg
    29941 tgacaaaagg tgagtcacta ttgacgatct atagtaacga cgaagaaatc acttctgtca
    30001 ttgaattatt atataaaaat attgaaattg gtgaatcggc aatgaagcca acgttgattc
    30061 atgacatcat cacagaataa aggaaaaaag cggactttac cgttttaaag aaaaggggaa
    30121 aagaaatgga actaaaccga atgatcgacc atactatttt gaaagcagac gcatctaaag
    30181 aagctgtgat gcaaattatc gaagaagcaa aaaaatacca tttttattct gtctgcatca
    30241 atccagcatg ggttgcgcta gcgaaagaac aattacaagg tgagccagta gccgtatgta
    30301 cagtaatcgg gtttcctcta ggagcaaaca cacccgagac aaaagcattt gaaacaacag
    30361 atgccatcaa taacggtgca gatgaagtcg atatggtcat caatatcggt gctttgaaat
    30421 caaaggatga tcaacaagtc caaaaagata tcgaagcagt tgttgaagca gcaaaagatc
    30481 gtgcgcttgt caaagttatt attgaaacat cgttattaga tagagaagaa atcatccgag
    30541 cttgcgaaat agcaaaagct gcaggcgctg attttgtcaa aacatcaact ggtttctcaa
    30601 caggcggagc aaaggtagag gatgtccgtt tgatgcgtga aactgtagga ccagaaatgg
    30661 gtgtcaaagc ttccggcggt atccataacg aagaagaagc aatggcaatg atcgaagctg
    30721 gtgctacaag aatcggaaca agcgcaggag tagcaatcgt atctggttca actggtgaag
    30781 gctattaatt ttttcaaagg agaagctcat ggcagaactc aaacaagaat ggatcaacac
    30841 agctattgaa gcgttagata aagcctatgt tccttattcc cattttccgg taggcgcttg
    30901 tctcgtaacg gaatcaggca agatttatca aggcatcaac attgagaatg catcatttgg
    30961 attaacgaat tgtgccgagc gcacagcttt tttcaaagca gtatctgaag gagaaaaaac
    31021 atttacgcat ctagtcgtag cggggcacac ccccgatcct atctctcctt gtggtgcatg
    31081 tcgacaagta atggcagaat tctgttcccc tgatatgccg gttacgctag taggagacaa
    31141 tggtgtgacg aaggcaacga ccgttcgtgc actgctgcct tatgcattta ctgaaaaaga
    31201 tctataactc atgttcattc agtcagctga ctgttgaaaa ataaaacaat tattttagga
    31261 ggctttacag aaatgaaaaa agcaaaatta tttggtttag gtgctgtcgc actagcagca
    31321 gggttatttt taggtgcatg cggcaacaac ggctctactg attcatccgg tggcaaaagc
    31381 tcatcagaca caacaactgc agcattgatc acagacacag gtggtgtaga cgaccgttca
    31441 ttcaaccagt cagcttggga aggactagaa aaatggggga aagatcaagg actttcacga
    31501 ggaaatgatg gcttccaata tttccaatct tcaaatgaat cagattacat cccaaatatt
    31561 gaccaagcat tgaatgctgg attcaaaacg atttttggta ttggttataa actaaaacca
    31621 gcgatccaag aacaagcaac aaacaataca ggtacgaact ttgttattat cgatgatgtc
    31681 atcgatggat tagataacgt tgtatctgca actttcaaag acaacgaagc ttcttatctt
    31741 gcaggtgttg cagcagctta tacgactgaa acaaacgtag tcggctttat cggcggtgta
    31801 aaaggtgaag taatcgatcg ttttgatgct ggattcaaag ccggtgttga tgcaggtgcg
    31861 aaagaattag gaaaagaaat caaagtattg aatcaatatg ctggtgactt ctctgctcca
    31921 gataaaggac gttcaattgc tcaaggtatg tatgcacaaa acgcagatat catcttccat
    31981 gcttcaggcg gtacaggtaa cggtgtattc caagaagcaa aatcattgaa cgaatcaggc
    32041 gacaaaaaag tttgggttat cggtgttgac cgcgaccaat cagacgaagg cgaatacaca
    32101 ttaaacggcg aaaagaaaaa cttcacattg acatctacac tgaaagcagt tggaacagta
    32161 gtagaagatt tggcacaaaa atcagcggac ggtaaattcc ctggcggcga acacactgtt
    32221 tatggattga aagaagacgg tgttggtcta acagaaggac aactttctga cgaagcgaaa
    32281 aaagcagttg atgaagcaaa agaaaaaatc atttcaggtg acgtaaaagt tccagaaact
    32341 cctgaagaaa actaaaacca tttctatagc cttgttagac acatgtttga caaggctatg
    32401 gtttgattag aacaagaatt gttcatttat ccaagctgcc ttgtgacagt ttggataagg
    32461 gaacaactcc ctgaagtaat gtttgtttca aaagaagaac gctcactaag cgtgaatctt
    32521 agtcaatcgt tgccctagat agaaatcgtt aggatgtgaa aaaaaggtgg ttcaagaaaa
    32581 tattgtcata gaaatgcaga atattaccaa agaatttgga acattcaagg caaatgacaa
    32641 catcaattta caagtaaagg caggagaaat tcatgcgttg cttggagaaa acggtgctgg
    32701 caaatctaca ttgatgaacg tgctttccgg attattagag ccgacatcag ggaaaatttt
    32761 gatgcgtggg aaagaagtaa agatcacaag tccgaccaaa gccaaccaat tagggattgg
    32821 gatggtccat cagcacttta tgcttgttga tgcctttact gtaacagaaa acatcgtgtt
    32881 gggaagcgag cctagtcgcg cagggatgct tgaccataaa aaagcacgaa aagagatcca
    32941 aaaagtttct gaacagtatg gattgtcagt caacccagat gcttacgttc gtgatatttc
    33001 agttggaatg gaacaacggg tagaaatttt aaaaacactt taccgaggag cagatgtact
    33061 gatttttgat gagccgacag ctgtattgac ccctcaagaa attgatgaat taatcgtgat
    33121 catgaaggaa ttagtcaaag aaggcaagtc gatcattttg attacgcata agttagatga
    33181 aatcaaagca gtagctgacc gctgtacagt tatccgccgt ggaaaaggaa tcggtacagt
    33241 caacgttaaa gacgtaacct cacaacaatt agccgatatg atggttggaa gaacggtttc
    33301 attcaaaacg atgaaaaaag aagcgaagcc tcaagaagtc gttttgtcta ttgaaaatct
    33361 agtggtgaaa gaaaatcgtg gattagaagc cgtgaaaaac ttgaacttag aggttcgtgc
    33421 tggcgaagta cttggtatcg ctggaatcga tggaaacggg cagtcggagt tgatccaagc
    33481 tttgactggt ttgcgaaagg cagaaagcgg acatatcaag ctaaaagggg aagacatcac
    33541 taataaaaaa ccccgaaaaa tcactgaaca tggtgtagga cacgtgccag aagaccgtca
    33601 taaatacggg ttagtcctag atatgacatt atctgaaaat atcgcactac aaacctatca
    33661 tcaaaaacct tacagtaaaa acggcatgct gaattattca gtaatgaatg aacatgccag
    33721 agaattgatc gaagaatatg atgttcgaac aacgaatgaa cttgttcccg caaaagcttt
    33781 atcaggcgga aatcagcaaa aagcaattat cgctcgtgaa gtcgaccgag atcctgatct
    33841 gttgattgtt gcaaatccaa ctcgtggact ggatgtaggt gcgatcgaat ttattcataa
    33901 acgtctgatc gaacaaaggg acaaatacaa agcagtgtta ctgatcagtt tcgaattaga
    33961 agaaatttta aatgtttcgg atcgtattgc tgttatccat gaaggagaaa ttgtcggtat
    34021 cgttgatccg aaagaaacat ctgaaaatga attaggtcta ttgatggcgg gctatacctt
    34081 agaagaagca cgaaaagagt tagaaaaggg ggctggcgca catgaatgat cgcagcgaaa
    34141 aattccgtaa tttattagtg cctgtacttt cagttgtttt aggattgatc ttaggcgcga
    34201 tattgatggc tgcttttggt tttcagccca tccaaggtta ttcttctatg ctagatgcag
    34261 cccttggttc gcaaagaggg ataggtgaaa cgttaaggca agcaacgccg ttgattttta
    34321 cagcattagg tttttctgta gctaactcag ctgggttttt caatattggg ctttctggac
    34381 aagcactttg cgggtggatc gtcagtgtct gggtggcact tgcgtttcca gatctgccaa
    34441 aagttgtttt gcttccgtta tgcgtgatct taggtgcctt agctggtgcg atggcagcgg
    34501 ctgttccagg tttacttcga gcattttttg gaacaagtga agtgatcgta acaatcatga
    34561 tgaactatat cttattatac gtaagcacat ttgctttaca tgaagtgatg cctgaaagct
    34621 atcgctcaag tttggactct tccaatatga tcagtcaaaa tgcctcactg cggttaccat
    34681 ggttaacaca agcttttggc ggctctcgcg taaactcagg attattttta gcaatcatcg
    34741 cgcttatttt cgtttggctg ctgatgaaga aaacgacttt aggatttgaa atccgttctg
    34801 ttggattgaa tccttttgct tctgagtatg caggaatgag tagtaaacgt acgatcgttt
    34861 tgtcgatggt catttctggc gctttggcag ggcttggcgg tgtggttgaa ggattaggaa
    34921 cgtatcaaaa cttctttgtt caaacgactt cgctatctat tggatttgac ggaatggctg
    34981 tttccttgct tggttctgga tcagcaatcg gtattctttt atcagcgatc ttgttcagta
    35041 ttttgaagat cggtggactt ggcatgcaga caggagcagg tgttccgttt gagatcgtca
    35101 atgtatcgat tgctttgatt atcttctttg tcgggatcaa cttcctgatt cgtttcgtca
    35161 tggctaaagt cttccaaggg aaaaaacaag aagagatcgt tgcgacaata gaatcgaaac
    35221 caacgaatca aaaccatgaa gggggcgaac tataatgtcg actatcgaat taattgcctt
    35281 gatccttact tctacactgg tttattcaac accgctaatt ctaacgtcac taggcggaac
    35341 tttttctgaa cgaagcggga tcgtaaacgt agggctagag ggaatcatgg tcatgggtgc
    35401 ttttagttcc atcgttttca acttgacgat gagtccatca cttggttcta tgacaccatg
    35461 gatcgctatt ttagtgggtg gcgtcgtagg tatcctattt tctctgattc acgcagtagc
    35521 aacagtcagt ttgcgtgctg accatattat cagcgggaca gtaattaatc tgatggcacc
    35581 tgctttaggt gttttcttga tcaaagcatg gtatggtaaa ggtcaaactg ataacatcac
    35641 gcagaatctg gggtattttt ctttcccagg attagcagat attccggtca ttggaccaat
    35701 tttctttaaa aatacattct tacctgctta ttgtgctatt ttgattgctg tcctagcttg
    35761 ggtgatcatc ttcaagacga aattcggctt gcgcctccgt tcagttggtg aaaatcctca
    35821 agctgccgac acattaggta tcaatgtata tgctatgcgt tattcaggcg tactgatttc
    35881 tggcttgtta ggcggtatgg gcggtgctgt ttttgctcag tcgatttccg gtaacttttc
    35941 agctggtacg atcgttggac aagggttcat ctccatggct gctatgatct ttggtaagtg
    36001 gaatccttta ggggcaatgg gtgcctcatt gttctttggt tttgctcaaa gcttgagtat
    36061 cattggcgca caattaccag ttatttcttc gattccgcca gtattgctgc aaattgctcc
    36121 atatttgctg acaatcattg tgctggttgt tttcttaggg aaagcttctg gtccaaaagc
    36181 aaacgggaag aactatatca aatcaaaata atcttttagc gaataaaaag ggtgggttac
    36241 gggaagtgtt tttcgtctcg ccctttttgt tttaaaggca ataaaaataa aaagaaagaa
    36301 ggaataatca tgtttaaacg cgtacatctg atcgtcatgg attcagtagg aattggagaa
    36361 gcaccagatg ccgataagtt cggtgatgtt ggttctgata cattaggaca cattgcgaaa
    36421 gaagcaggat tgaacgttcc acatttagag cagcttggtt taggaacgat ccgtcctctt
    36481 gaaggtgtgg aatcagtgaa agatcatcaa ggctatgcga cgaaattaga agaagtttct
    36541 gtagggaaag atacgatgac aggccactgg gaaatcatgg gattgaatat caaaactcca
    36601 tttcgtgtgt ttcctaatgg attccctgat gatctgatca aacaaatcga agaattttct
    36661 ggccgtaaag tcgtatgtaa caaaccttat agcggaacag ctgttattga cgattacggt
    36721 gaacaccaaa tgaaaacagg cgatttaatt gtatatactt ctgccgatcc tgtccttcag
    36781 atcgcggcac atgaagaaat cattccttta gaagaattat atcggatttg ccaattcgtt
    36841 cgtgatataa caaaagatga tccgtatatg attggccgaa tcattgcacg tccgtacgtg
    36901 ggtgaaccag gagacttcac acgaacaagc aatcgccatg attatgcgtt gaatccattc
    36961 ggacacacag tccttgattc tttgaaagaa gcaggaaagg acgtgattgc tgttggaaag
    37021 atcaacgata ttttcaacgg acaaggaatc actgaatctg tccgcacgaa aagcaatatg
    37081 gatggcgttg accagctgct gaaagttatg aaaaacgatt tttcaggaat cagctttacc
    37141 aatttagtag attttgatgc attatacggt catcgtcggg atgtagtcgg ctatgcgcat
    37201 gcgattgaag aatttgactt acgaattcca gaattgcttg aagcgatgga agaagacgac
    37261 ttgctaatga ttacagccga tcacggaaac gatccgacgt ttactggtac cgatcatacg
    37321 cgagaatatg taccgttatt ggtttacagt aaaaaaatga aagaacaagg ggcattgcct
    37381 caaggatatt attcagatat ttcagcaacg attgctgaga actttagtgt ttcttcgact
    37441 gaaaatggcg aaagcttctt gaaattatta aaataatctt tttaaatagg agaaagcaaa
    37501 tgaccaagtt aagcgagatg ttacaagaaa cgacccagtt tatccttgat aaaggagtaa
    37561 aagaaattga ttttggtttg attctaggtt caggattagg tgaattagca gatgaaatcg
    37621 aagatgctgt aatgatttct tatgatgaga tcccgttctt tcctgtttcg acagtagttg
    37681 ggcatgcagg aaagctagtt tatgggtctc tggcaggcaa gaaagtgtta gccatgcaag
    37741 ggagattcca ctactatgaa ggtcattcca tgcaaacagt gacctatcca gtacgtgtga
    37801 tggccggact aggggcccat tcagtcattg tgactaatgc atgtggtggt gtcaacgaat
    37861 catttgtacc aggagatttg atgctgatta ccgatcatat caattttact ggtgaaaatc
    37921 ctttgatcgg tccgaatgaa gatgcaatgg gccctagatt tccagatatg agtcaagctt
    37981 atacgcctga ctatcaagca acagcaaaag cagcagcaga aaaactaggt ttggatctta
    38041 aagaaggcgt atatatgggc tattctggac caacttatga aacgcctgcg gagatccgaa
    38101 tgtctcggac aataggagcc gatgcagtag ggatgtcgac tgttccagaa gtcatcgtag
    38161 cagctcacag cggattaaaa gtgttaggaa tctcttgtat taccaactta gcagcaggta
    38221 tgcaagcgaa tctgagccat gaagaagttg tagaaacaac tcagagagtc aaacaatctt
    38281 ttaaagcatt ggttaaagaa acattgacat tattgtaata ggaggaacaa taaaatgagt
    38341 gtgcatattg aagcgaaaga aggcgagatc gccgataaga ttttacttcc gggagatcct
    38401 ttgcgagcta aatatattgc ggagactttt ttggaagatc cagtttgtta caatcaggta
    38461 cgcggtatgt taggctttac cggcaaatat aaaggagaac gtatttctgt tcaaggaaca
    38521 ggaatgggaa tgccatcagc caccatctat gcccatgaat tgatccagtc gtatggtgta
    38581 aagaaattga ttcgagtagg tacatgcggt gcattgtcaa aagacgtcca tgttcgtgat
    38641 ttagtactag cacaaggtgc agcgaccagt tcttcgatga tcgaaaaaaa tttccaagcc
    38701 tttcattttc ctccaatcag tgatttcaat ctattactga aagcatatga gatagctaaa
    38761 gaaaagggct atacggttca tgtaggaaat gttctttcgg aagattcttt ctacaaagat
    38821 gatttgacag agacgttcca attagctgaa cttggcgttt taggagtgga aatggaagca
    38881 gcggctcttt attacttagg cgcgaaatac catgtgcaga cattaagttt gatgaccgtt
    38941 agcgaccatt tgatcaccgg tgaagaaaca acagctgcag aacgtcaatc tacgttcaat
    39001 gaaatgatcg aagtaggatt ggaaacagcg atagcagaat aagaaggaaa tgaaaaagct
    39061 gccctgcatt aagtaataag aacatccaag caaaaatagc tcctcatgtt tttgacttgg
    39121 ctggacttat tattgcaaat gtaagctttt gaacaccgtt taccaagagg ttgtgaatct
    39181 gccgtttaac tctgagtcac aaggaacaat ttcaaaaaac agcttttcat gtttgatgaa
    39241 tattgtgact tgtgaccgag aagttggcag gagaaaaccg tttatacgaa aaaagggact
    39301 gtgacaaatc gtgtcacagt ccctttttta tagtgttttt tataggtagt attttttagc
    39361 aactgttaag tcatcattta attcataaac aagtggttga cctgttggaa tttcaagatc
    39421 catgatgtct tcgtcagaga taccttcgat atgtttagct agggcgcgta atgagttacc
    39481 atgtgcagct actaaaacag ttttgttgtc tagtaatgct ggagcaattt catcttgcca
    39541 gaatggcaaa gcgcgttcta atgtcacttt caagttttct ccaccaggaa tatcgcgttt
    39601 gtctaacatt gcgtaacgac ggtcatttgc tgcagaacct tcgtcagttt cttccattaa
    39661 tggaggaaga gtatcgtaag aacgacgcca gatgtgcact tgttcgtcgc cgtatttttc
    39721 agctgtttct tttttgttca aaccttgtaa tttaccatag tgacgttcgt ttaagcgcca
    39781 tgatttgatt tgtggtaccc aaagttgatc tgaatattct aagatcaagt tacatgtttt
    39841 gattgcacga gttaaaacag aagtgtaagc aacatcaaat tcgattccag cttctttgat
    39901 tttgcgtccg ccttcttttg cttcttcgat tccttctggt gctagattta catcagccca
    39961 accagtaaat tggttcaatg cattccattc actaagaccg tgacgagaaa aaactaattt
    40021 tggcatgttt agctctcctt ttaagatagg tttatatttc tgaaattttc agttctgttt
    40081 tattgtacaa ttttttggaa gagatttcca tcgaaaaatt agataagatt ctatgagaaa
    40141 acaagtagag aatataaaaa aacgaacagt gcccacgcaa ctgttcgttt ttaggtatta
    40201 gatgcataat tgctcgattt ctttttcagg taaattgata tatttataac atgttccaac
    40261 tgaaacaccg cattttgaag aaataaattg gatcgtttct tttttttcat aatagagacg
    40321 tcggattttt ttgatcgttt ttgcatcgat ttttggtctt ccgccgattt ttcctttttt
    40381 acgagcttca tctaatccaa ctaatgtacg ttctttgatc aatgcgcact ccatgtttgc
    40441 taggttttcc attaattgga aatagatttt tcccatttcg cgcgtatcga ttccttcatc
    40501 aagactaatc aagtgcagcc cgcgatcttt gaataattgc gtgagttctg ttaattgccg
    40561 cgtagatttg cctaatcgat gaagttggca aatgactagt gcatcgcctt gttgcatgtc
    40621 agcgatcact gactccaaat gaacagcagt agaatcattt tgttccactt catatgtctc
    40681 atggaaaatt tcttgacaac caaagtcaga taattgatca acctgagttg ttaaatcaac
    40741 gtcatttatg gttgtgcgtg cataaccaat aattttcatg gtgattcctt tctagcagtg
    40801 attttctaac tggatataac agtaaaaatg tagcataaag tacgtcaaaa agatagtttt
    40861 ttcgcaaatt ttccgctgtc tttttttatt ggatgatcaa gcagtttttc tttatttacg
    40921 acggattgcg tcaagaatct caacgccttt ttctgagccg acaactactt tagaaaccat
    40981 gtttaggaaa agcccatgtt ctactacccc aacaagttca tctaaatagg tagctaagct
    41041 ttctggctgt tcgatgactt ctagatgcag atcaatgatg taatgaccac catctgtggt
    41101 taagacttct ccgttttcgt ctgtacgcaa gacgggttgg aatctttttt cttcaaaaat
    41161 atgaagtagc tgttgggagc cataagggat cacttcaact gggagtggga acttcccaag
    41221 tttatcgact aatttagaag aatcaacgat ccaaatcgtt tcttttgagt acgtagcgac
    41281 tattttctcg aaaagcaaag cagcgccgcc gcctttgatc ccttgaaaat cagcactgat
    41341 ttcatcagca ccgtctattg tcaaatcgac tacggggact tcatcaatac tctttaacgg
    41401 tattcctaat ttttccgctt gttcttttgt cgcatttgaa gtagtaactc ctgtgatacg
    41461 taaaccttct tcacgcatac ggcgaccaat ctcttctacc atataatagg cggttgaacc
    41521 ggtcccgaga cctacgatca tcccgtcctt tacgaatttc gcggcttcaa tgccagctaa
    41581 ttgttttaaa ttcatctttg cgtccacctt tttttagttt gagcatcgtg ctttcctcac
    41641 tattttagcg tgatttacgg taaaaagata ccattctttt ttattccaat aaagcttttt
    41701 caaaaaaaat tgcatctctt gttgacatgt tttttcattc gtgatattct aacaaaggtg
    41761 ctttatctac aggtttctgt gaagaaacgt gctgactgtt tatcaaagca tatttgataa
    41821 ggatgcagac attgcattta ttttttatca caaaaaaaga accacctgga tgtgtggaac
    41881 tagacgcaaa tcgtgggaag gaggaaacaa ggaatgccta caattaacca attagtacgt
    41941 aaacctcgta aatcaaaggt ggaaaaatct aattcacctg ctttgaacaa aggatataac
    42001 agctttaaga aaactcaaac taacgtaaac tcacctcaaa aacgtggggt agcaactcgt
    42061 gtgggtacaa tgacacctaa aaaaccgaac tcagctttac gtaaatatgc acgtgttcgt
    42121 ttgtcaaact taattgaagt tactgcttat atcccaggga ttggtcataa cttacaagaa
    42181 cacagtgttg tattattacg cggtggtcgt gtaaaagact taccaggggt acgttaccat
    42241 atcgttcgcg gtgctttaga tactgctggt gttaacgacc gtaaacaaag ccgttctaaa
    42301 tacggaacta aaaaacctaa agcttaattt taattattaa acaaatgaaa cttgcttcta
    42361 ctatatagaa gtaattacta tgaacaatat ttcggaagga ggagttacgg atgccacgta
    42421 aaggtcctgt tgcaaaacgt gatgttttac cagatcctat ttataactca aaattagtaa
    42481 ctcgcttgat caaccgtgta atggttgacg gaaaacgcgg gattgcagct aacattatct
    42541 ataatgcctt tgacttgatc aaagaatcta caggtaacga tccattagaa gtgtttgaac
    42601 aagcaatgaa aaacgttcaa cctgttcttg aagttaaagc acgtcgtgtt gggggttcaa
    42661 actaccaagt accagttgaa gttcgccctg aacgccgcgt tactctaggt cttcgttggg
    42721 tagtaaacta cgctcgcttg cgcggtgaac acacaatgga agaacgctta gcaaaagaaa
    42781 tcatggatgc tgcaaacaac acaggtgctt ctgttaaaaa acgtgaagac actcataaaa
    42841 tggctgatgc caaccgtgcg tttgctcact atcgctggta agattctctc tgtttggtcg
    42901 gcttttataa gtcgacctca gatagtgtcc ttatttttag caactagata gtaaagagag
    42961 gagaaacaag gagaatgacc agagaatttt ctttacaaaa cactcgtaat atcggtatca
    43021 tggcccacat cgatgctggt aaaacgactg ctactgaacg tatcttgtac tatactggta
    43081 aaatccataa aattggtgaa actcatgaag gagcttcaca aatggactgg atggaacaag
    43141 aacaagaacg tggtattacg atcacttctg ctgctactac agcacaatgg aagggtcacc
    43201 gaatcaacat catcgacaca ccaggacacg tagacttcac agttgaagtt gaacgttcat
    43261 tgcgtgtact tgatggtgct gtaactgttc ttgatgcaca atcaggtgtt gaacctcaaa
    43321 ctgaaacagt ttggcgtcaa gcaacaactt atggtgtacc tcgtatcgta tttgcaaaca
    43381 aaatggacaa gatcggtgct gacttcttat attcagtatc aactttacat gaccgtttgc
    43441 aagcaaacgc tcacccaatc caattaccaa ttggtgcgga agacgacttc acaggaatta
    43501 tcgaccttgt aacaatgaag gctgaaatgt atacaaacga tcttggaaca gagatcgaag
    43561 aaacagaaat tcctgaagaa taccgcgaat tagctgaaga atggcgcgaa aaattggttg
    43621 aagctgtagc tgaaactgat gaagaactta ctttgaaata cttagaaggt gaagaaatca
    43681 ctgaagctga gttaaaagaa ggtatccgcc gcgcaacagt taacgtagaa ttctacccag
    43741 tactatgtgg ttcagcattt aagaacaaag gggttcaatt actattggac gctgttcttg
    43801 attacttgcc atcaccgtta gatatcccag ctatcaaagg gattgaccct aaaactgatg
    43861 aagaagtaga acgtcctgca gacgacagcg ctcctttctc agctttagca tttaaagtta
    43921 tgactgaccc attcgtaggt cgtttaactt tcttccgtgt gtactctggt gttttacaat
    43981 caggttcata cgtacaaaac gcaacaaaag gcaaacgtga acgtgttggt cgtatcctac
    44041 agatgcatgc caactctcgt tcagaaatct ctgaagttta cgctggagat atcgctgctg
    44101 ctgttggttt gaaagacaca actactgggg acacactatg tgatgaaaag agtctagtta
    44161 ttcttgaatc catggaattc ccagaaccag ttatccaagt cgctattgaa cctaaatcaa
    44221 aagctgacca agataaaatg ggtgtggctt tacaaaaact atctgaagaa gatcctactt
    44281 tccgtgctga aacaaacgtg gaaacaggcg aaacaatcat cgctggtatg ggtgaacttc
    44341 acttagatat catcgttgac cgtatgcgtc gtgaattcaa agttgaagct aacgtaggtg
    44401 cgcctcaagt atcttaccgt gaaactttcc gtgctggcac tcaagccgaa ggtaagtttg
    44461 tacgtcagtc tggtggtaaa ggtcaatacg gtcacgtatg gattgaattt acaccaaatg
    44521 aagaaggtgc tggtttcgaa tttgaaaatg cgatcgtcgg tggggttgtt cctcgtgaat
    44581 acattccagc tgttgaaact ggtttgaaag atgctatgga aaatggtgtc ttagctggct
    44641 atccattagt agatatcaaa gctaaacttt acgatggttc ataccatgat gtcgattcaa
    44701 atgaaactgc cttccgtgtt gctgcttcta tggcattacg tgcagcagct aagaaagcta
    44761 atccagtaat ccttgaacca atcatggctg tagaagttgt tatccctgaa gattatttag
    44821 gtgatgttat gggtcacgta actgctcgtc gtggacgtgt tgaaggtatg gaagctcgcg
    44881 ctggcggcca acaagttgtt cgtgcgatgg ttccacttgc tgaaatgttt ggttatgcta
    44941 caactcttcg ttctgctact caaggtcgtg gtacatttac aatgacattt gaccactacg
    45001 aagatgtacc aaaatcagta caagaagaaa tcattaagaa aaacggcgga aaagctgaat
    45061 aatttcttaa aaccggatag tcctgcaaag agattatcca aagagactat atttttaata
    45121 tagtttcgtg taaaataatt atcgatgata tgggcatgtg gtgtaccttg tgtacatcat
    45181 atacctatca gacaaataaa aaatatattg tatttttagg aggacatttt aaaatggcaa
    45241 aagaaaaatt tgaccgttct aaaccacacg ttaacattgg tactatcgga cacgttgacc
    45301 atggtaaaac tacattaaca gctgcaatca caactgtact atctaagaaa aacggcggcc
    45361 aagctatggc ttacgatcaa atcgatggtg ctccagaaga acgcgaacgc ggaatcacaa
    45421 tctcaacagc tcacgttgag tatgaaacag atacacgcca ctacgctcac gttgactgcc
    45481 caggacacgc ggattacgtt aaaaacatga tcactggtgc tgctcaaatg gacggagcta
    45541 tcttggtagt ttctgctgct gacggcccaa tgcctcaaac tcgtgaacac atcctattgt
    45601 ctcgtcaagt tggtgttcct tacatcgttg tattcttgaa caaagtagac atggttgatg
    45661 acgaagaatt actagaatta gttgaaatgg aagttcgtga cctattaaca gaatacgaat
    45721 tccctggtga cgatgttcct gtagttgctg gatcagcttt gaaagctcta gaaggcgacg
    45781 cttcatacga agaaaaaatt cttgaattaa tggctgcagt tgacgaatac atcccaactc
    45841 cagaacgtga caacgacaaa ccattcatga tgccagttga agacgtgttc tcaattactg
    45901 gacgtggtac tgttgctaca ggtcgtgttg aacgtggaca agttcgcgtt ggtgacgaag
    45961 ttgaagttgt tggtattgct gaagaaactt caaaaacaac agttactggt gttgaaatgt
    46021 tccgtaaatt gttagactac gctgaagctg gagacaacat tggtgcttta ctacgtggtg
    46081 ttgcacgtga agacatccaa cgtggacaag ttttagctaa accaggtaca atcacacctc
    46141 atacaaaatt ctctgcagaa gtatacgtgt tgacaaaaga agaaggtgga cgtcatactc
    46201 cattcttcac taactaccgt ccacaattct acttccgtac aactgacgta acaggtgttg
    46261 ttgaattacc agaaggaact gaaatggtta tgcctggcga caacgtaaca atggaagttg
    46321 aattaatcca cccaatcgct atcgaaaacg gtactaaatt ctcaatccgt gaaggcggac
    46381 gtacagttgg tgccggtgtc gttacagaaa tcaaagctta attcacttaa gttttaaagt
    46441 aaagcaacat ttattcggac gtaatcgctc ggaacgaagg tcttaggatc tttgttccga
    46501 gcttttttat ttattgcagg atcaggaaaa aagacaggag tattataaat agaagaaaca
    46561 cgaaataaaa gagtcagtat tttctaaatg aaactttatg aaaacaaaat gttttttaca
    46621 agaaaaagcc ttgctttgtg agtttatata tattataatg acaaaggtgc gtttacttta
    46681 gtgaattgca cagacgtaat cagaatgatt tcgtcggcta agaaacgaga ggttgcgaca
    46741 cgcccggacg ctttgccacg aacgagcgtg acggaaattt tcgtggagct atgtctactt
    46801 ttcaaaatag gcgaaggagg gaacaagatg gcaaaacaaa aaattcgtat ccgtttaaaa
    46861 gcgtatgaac accgtatttt agatcaatca gcggataaaa tcgtggaaac tgcaaaaaga
    46921 actggagcta gcgtgtctgg tccaattcca ttaccaactg aacgcagtct ttacacaatc
    46981 atccgtgcga ctcataaata caaagattca cgcgaacaat tcgaaatgcg tacacacaaa
    47041 cgtcttatcg acattgtgaa cccaacacca aaaactgttg atgctttgat gaagctagac
    47101 ttaccatctg gtgtaaacat cgaaatcaaa ttataaaact aaaacaaaaa taatggaggt
    47161 gtactcatga ccaaaggaat cttagggaaa aaagtgggaa tgacacaaat cttcactgaa
    47221 tctggtgaat taattccagt aactgttgta gaagctactc caaacgtagt actacaagtg
    47281 aaaacaatgg aaacggatgg ctacgaagct attcaagttg gttaccaaga tatgcgtgaa
    47341 gttttatcaa acaaacctgc gaaaggtcat gttgcaaaag caaacacggc tcctaagcgc
    47401 ttcattcgtg aattcaaaaa tgttgagcta ggagaatatg aagtaggaaa agaaattaca
    47461 gtagatgttt tccaagcagg agacattatt gatgtaacag gtactacgaa aggtaaagga
    47521 ttccaagggg ttatcaaacg ccacggacaa agccgcggac caatggctca cggttctcgt
    47581 taccatcgtc gtcctgggtc aatgggtcca gttgcaccta accgtgtatt taaaaacaaa
    47641 cgtcttgctg gccgtatggg tggcaaccgc gtaactatcc aaaacttgga agttgtacgt
    47701 gtcgatgcag aaagaaatgt tatcttgatc aaaggtaatg ttcctggagc taaaaaatct
    47761 ttaattacta tcaaatcagc tgtgaaagct aaataattgt aggaagaaag gaggaactaa
    47821 ggaatgccga atgtagcatt attcaaacaa gatggaagcc aaaacggtga aatcacactt
    47881 aacgaagaaa tcttcggaat tgaacctaat gaaagtgttg tttacgatgc aatcgtaatg
    47941 caacgtgcgt cattaagaca aggaacacac gcggttaaaa accgtagcgc tgttcgcggc
    48001 ggcggacgta aaccatggcg tcaaaaagga acaggtcgtg cacgtcaagg atctatccgc
    48061 tcaccacaat ggcgtggagg tggcgtagtc tttggaccaa caccacgttc ttacagctac
    48121 aaacttccta aaaaagttcg tcgtttagca atgaaatctg tattgtcaga aaaagttgct
    48181 gaaaacaact tggtagcaat cgaaggcttg aacttcgacg caccaaaaac aaaagaattc
    48241 aaacaagttc ttgctaactt atctatcgac tctaaagtat tggttgttct agaaaaaggt
    48301 aacgactttg ctgctttatc agcacgtaac ttaccaaacg tttctgtagt aacttctgat
    48361 aatgtgagtg tgctagacgt tgtatcaaat acaaaagtgt tagcaacaca aactgctctt
    48421 actcaaattg aggaggtgct tgcataatga acttactaga cgtaatcaaa cgcccagtga
    48481 tcactgaaaa atcaatgctt gccatggatg acaagaaata cacttttgaa gtggacactc
    48541 gcgcaaacaa aactttagta aaacaagcgg tagaagctgc ttttgacgtt aaagtcaaaa
    48601 acgtgaacat cgtgaacgta cgtgcgaaat tcaaacgtat gggcaaatat gcaggatata
    48661 caaaaaaacg tcgcaaagcg atcgttacat tgactgaaga ttcaaaagaa atccaattat
    48721 tcgaagctgc tgaataagca ctaatagata actcattaaa ttaggaggga aaaacgtggc
    48781 gattaaaaag tacaaaccta cctcaaatgg tcgtcgtaac atgacaggtt ctgattttgc
    48841 tgaaatcaca acatcaacac ctgaaaaatc attgttacaa ccattgaaaa accatgctgg
    48901 acgtaacaac aacggccgca tcactgtacg tcatcaaggt ggcggacaca aacgccaata
    48961 tcgcgtgatc gacttcaaac gtaacaaaga taacgttgtt gcaactgttc aaacgatcga
    49021 atatgatcca aaccgttcag ctaacatcgc gttagtacac tatgaagatg gagtgaaagc
    49081 ttacatctta gcacctaaag gactagaagt gggtatgaaa cttgtttcag gtccagaagc
    49141 agatatcaaa gtcggaaatg ctcttccatt agaaaacatt ccagttggta ctgttatcca
    49201 caacattgaa atgaaacctg gtaaaggtgg acaattgatc cgttctgctg gtacaagtgc
    49261 tcaagtactt ggtaaagaag gcaaatacgt attgatccgc ttgaactctg gtgaagttcg
    49321 tatgatctta gctgcatgcc gtgctacaat cggttctgta ggtaacgaac aacacgaatt
    49381 aatcaacatc ggtaaagctg gacgctctcg ttggatgcgt aaacgcccaa ctgtacgtgg
    49441 tagcgtaatg aaccctaatg atcacccaca cggtggtggt gaaggtaaag ctcctatcgg
    49501 acgtaaagca ccagtttctc cttggggtca accagctctt ggttacaaaa ctcgtaacaa
    49561 aaaagctaaa tcagacaaac ttattgttcg tcgtcgcaaa aataaataat tactttgacc
    49621 atgtgtcgca cattttgaga ggaggttcac catgggtcgt agtttaaaga aaggaccttt
    49681 tgtcgatgag catttaatga aaaaagttga agctcaagca ggggttgaaa agaaaaaagt
    49741 tatcaaaact tggtcacgcc gttcaacagt tttcccaagt tttgttggat ataccatcgc
    49801 agtttatgat ggacgtaaac atgtaccagt ttacatccaa gaagacatgg taggacacaa
    49861 attaggtgaa tttgcaccta caagaactta tcgtggacat gctgctgacg ataagaaaac
    49921 gaaacgctaa tttgagggga ggataaatcc aaatggcaga acaaatcaca tcagctaaag
    49981 caactgcaaa aacagttcgc acttcacctc gaaaagcccg tttagtaatt gaccttatca
    50041 gaggtaaaag tgtcgcagat gcaatttcaa tcttgaaatt cacaccaaac aaatctgctg
    50101 gaatcattga aaaagtattg atgtcagcag ttgcgaatgc agaaaataac tttgacttag
    50161 atgttgaaaa cttggtagta agcgaagctt ttgttaacga aggacctaca atgaaacgtt
    50221 tccgtcctcg tgcaaaagga tcagcttctc caatcaacaa acgtacaagt catatcacgg
    50281 tagtcgtatc agaaaaataa ggagggataa tcagtgggtc aaaaagtaca tccaattgga
    50341 atgcgtgtag gcatcatccg cgactgggat gctaaatggt atgctgaaaa agagtatgct
    50401 gagttcttac acgaagattt aagaatccgt aaatttattt caactaaact tgctgatgct
    50461 gctgtatcaa cagttgaaat cgaacgcgca acaggtcgcg taaacatttc aattcacaca
    50521 gctaaaccag gtatggttat tggtaaaggc ggttctgaag tcgaaagcct aagaaaagaa
    50581 ttaaacaaat taactggtaa aagaatccat atcaatatcg tggaaatcaa aaaaccagat
    50641 ctagatgcaa aattagtagg tgaaggaatc gcacgtcaat tagaaaaccg tgttgctttc
    50701 cgtcgcgctc aaaaacaagc catccaacgc tcaatgcgtg ctggcgctca agggatcaaa
    50761 actcaagtat ctggtcgtct aaacggagca gatatcgctc gttcagaagg atactcagaa
    50821 ggaactgttc ctcttcatac tttacgtgcg gacattgatt acgcatggga agaagcagat
    50881 acaacatacg gaaaactagg agttaaagtg tggatctatc gtggagaaat tcttccaact
    50941 aaaaaaaaca ctgagaaagg agggaaataa tcatgttagt acctaaacgt gtaaaacacc
    51001 gtcgtgaatt ccgcggaaaa atgcgcgggg aagctaaagg cggaaaagaa gtagcattcg
    51061 gtgaatacgg tttgcaagct gttgattcac attggatcac aaaccgccaa atcgaagctg
    51121 ctcgtatcgc aatgactcgt tacatgaaac gtggtgggaa agtatggatt aaaattttcc
    51181 ctcacaaatc ttatactgcc aaagcaattg gggtacgtat gggttctggt aaaggggcac
    51241 ctgaaggatg ggttgcacca gtaaaacgtg gtaaaatcat gtttgaaatc gcaggcgttc
    51301 ctgaagaagt agctcgtgaa gcgttacgtc tagcttctca caaattacca atgaaaacta
    51361 agatcgtaaa acgtgaggaa atgggtggtg aatcgaatga aggttaaaga aatcagagaa
    51421 ttaaccactg ccgaaatgct tgatcaagaa aaacaattaa aagaagaatt gtttaatctt
    51481 agattccaat tagcaacagg tcaattagaa aacactgcac gtattaaaga agtacgtaaa
    51541 tcgattgcac gcatcaaaac tgtgttgcgt gaacaagcta agtaattatg gaaggaggcc
    51601 attaagcgta tgactgaagg aagaaatcaa cgcaaagttt atcaaggtcg cgtggtttca
    51661 gacaaaatgg ataaaacaat taccgttgct gtcgaaacaa agaaaaacca ccctatctat
    51721 ggcaaacgca tgaactattc taagaaatac aaagtgcatg atgaaaacaa cacagcaaaa
    51781 gttggcgaca tcgtgagaat tatggaaact cgtccattat cagctacaaa acgtttccgt
    51841 ttgttggaag tagtcgaaga agcagttatt atctaataac acgagatttt cctatataga
    51901 ttgaatgcaa aatctgaaag gaggatacac aacgtgatcc aagcagaaag tcgtttaaaa
    51961 gtcgctgata actcaggcgc acgtgaaatt ttgacgatca aagtccttgg tggatctggt
    52021 cgtaaaactg ctaacatcgg tgatgtgatc gttgctacgg ttaaacaagc aacgccaggt
    52081 ggggttgtca aaaaaggtga cgtcgttaaa gccgttatcg ttcgaacaaa atcaggagct
    52141 cgtcgtacag acggttcata tattaaattt gatgaaaatg ctgcagtgat tattcgtgat
    52201 gataaaagcc ctcgtggaac acgtatcttt gggccagttg cacgtgaact acgtgaaaac
    52261 aacttcatga agatcgtttc actagcacca gaagttttat aatcttgaga cacttatcaa
    52321 aggaggtgcg aaacagttat gtttgttaaa aaaggcgata aagttaaagt tatcactgga
    52381 aaagacaaaa acaaagaagg cgttgtacta gcagcgttcc cgaaaaaaga taaagttatt
    52441 gtcgaaggtg taaatgtcgt taaaaaacac caaaaacctt ctcaagctgc tcctcaaggt
    52501 ggaattgttg agatggaagc gccaattcat gtttctaacg tgatggtggt tgactctact
    52561 ggtgtagctg gccgagttgg ctacaaagaa gtagacggca aaaaagtccg tgtttctaaa
    52621 aaaaccggtg aagttttaga taaataaaag tttaaggaag gagggtctta ctgaatgaac
    52681 cgcctaaaag aaaaatatct taaagaagta actccatcat tgatggaaaa atttaactat
    52741 agctctgtta tgcaaacacc taaagttgaa aaaatcgtca tcaacatggg tgttggtgat
    52801 gcggtatcaa acgctaaaaa cttagataaa gcagttgaag aattaacttt gatcactggt
    52861 caaaaaccaa tgattacaaa agctaaaaaa tcaatcgctg gattccgttt acgtgaagga
    52921 atgccaattg gtgcaaaagt tacattgcgc ggagaaagaa tgtacgaatt cttagacaaa
    52981 ttggtttctg tttcactacc acgtgtacgt gacttccatg gtgtaagtaa aaaagccttt
    53041 gacggtcgtg gaaactatac tttaggtatt aaagaacaat tgatcttccc agaagtagat
    53101 tatgatttag tagataaagt acgtggtatg gacatcgtta ttgtaacaac agcaaactct
    53161 gatgaagaat ctcgtgagtt gttgacacaa ttaggcatgc cattccaaaa ataattaaag
    53221 gaggcgaact acgtggctaa aaaatcaatg attgctaaaa acaaacgtcc tgcaaaattt
    53281 tcaacacaag cttatactcg ttgcgaacgt tgcggacgtc cacattcagt ttatcgtaag
    53341 ttccatcttt gccgtatttg cttccgcgaa cttgcctata aaggtcaaat tcccggcgtg
    53401 aagaaagcta gctggtaaaa agtaaattcg cataagaagg aggtaaaaga tcaatggtca
    53461 tgacagatcc aattgcagat tttctaactc gtatccgtaa cgcaaacatg gttaaacatg
    53521 agtctttaga agtacctgca tcaaaaatta aacgtgacat cgctgaaatc ttgaaacgtg
    53581 aaggtttcgt tcgtgatgtt gaatacatcg aagatgacaa acaaggcgtg atccgtgttt
    53641 tcctaaaatt tggtaaaaac ggcgaacgtg ttatcacaaa cttaaaacgt atctctaaac
    53701 caggtttgcg tgcttatgtc aaagctgacg aagtaccaaa agtattgaac ggtttaggta
    53761 tcgcaatcat ttcgacttct gaaggtgttg tcactgataa agaagctcgt gaaaaaaata
    53821 tcggcggcga agttatcgct tacgtatggt aatcaagtag aatctacaag gaggtgtctt
    53881 taagtgagcc gtatcggaaa taaagtcgtt gttatccctg aaggagtaac agttactcaa
    53941 gacggaaaca acgttacagt taaaggacca aaaggtgaat tgactcgtac tttttcagca
    54001 gatatcaaaa tgaatatcga aggaaacgaa gtaactttca ctcgtccaaa cgacagtaaa
    54061 gaaatgaaaa caattcatgg aacaactcgt gcgaacttca acaacatggt tgtaggtgtc
    54121 agcgaaggtt tccaaaaagc gttagaacta atcggggttg ggtaccgtgc acaattacaa
    54181 ggatcaaaac ttgtattgaa cgttggttac tcacatccag tagaaatcac accaccagct
    54241 ggagtaactg ttgaagtacc agctaacaca caagtaattg ttaaaggtgc gaacaaagaa
    54301 gaagttggcg aattagctgc aaacattcgt ggaactcgtc ctccagaacc ttataaaggt
    54361 aaaggtattc gttatgttgg cgaatatgta cgccgtaaag aaggtaaaac tggtaaataa
    54421 tcgtttagaa aaattgcata gaagccagca gcaggaaaaa ggcatttggc tgaagtttac
    54481 cttcaaaaaa atacattttt acttgtgccg cagcttctat ttttcgaaac gtatttactg
    54541 cttgaaatag tagtacaata aaaataaaga ggtgacaatt gtgattacaa aaccagataa
    54601 gaacaaaaca cgtcaaaaga gacatcgtcg tgtacgtaac aatatctctg gtactgctga
    54661 gcgcccacgc ttgaacgttt ttcgttctaa caaaaacatc tacgctcaaa ttattgatga
    54721 cgtagcgggt gtaacgctag caagtgcctc taccttggat aaagaaattt caggtggaac
    54781 aaaaacagaa gcagcacaag ctgtcggtaa actagttgct gaacgtgcag ctgaaaaagg
    54841 cattaaagta gtagtctttg accgtggtgg atacctttac catggccgtg tgcaagcttt
    54901 agctgaagct gctcgcgaaa atggactaga attttagaag aaggaggaat accattcatg
    54961 gtttatatcg atccaaaaca tttggaatta gaagaccgcg ttgttgcaat taaccgtgta
    55021 acaaaagttg ttaaaggtgg acgtcgtcta cgttttgctg ctttagttgt tgtcggtgac
    55081 aaaaacggac acgtagggtt tggtacaggt aaagctcaag aagttcctga agcaatccgt
    55141 aaagcaattg aagatgcgaa gaaaaactta gttgaagtac ctatggttgg ttcaacaatc
    55201 ccacacgaag taatcggtgc ttttagtggt ggtagaatcc taatgaaacc tgctgtagaa
    55261 ggttctgggg ttgctgctgg tggaccagtt cgtgccgtat tagaattagc tggggtagca
    55321 gatatcactt ctaaatcttt aggctcaaat acaccaatca acgttgttcg cgcaacagtt
    55381 gaaggtttga aacaattgaa acgtgcagaa gaagttgcag aacttcgcgg caaatcagtt
    55441 gaagaattaa tcggataagg aggacaacaa taatggctga attaaaagtt actttaaaac
    55501 gcagtattat cggacgtcct caaaaccaac gcgatacagt taaagcgtta ggtcttacaa
    55561 agattaacag ttcagtggtt aagccttcta atgaagcaat taaaggcatg atcaatactg
    55621 tttctcacct agtggacgtt gaagaaattt aatttagaaa aatcataact agaacattaa
    55681 ggaggtgcca aacctatgaa acttcatgaa ttaaaacctg ctgaaggttc acgccaagta
    55741 cgtaaccgtg ttggtcgtgg tacttcatct ggtaacggaa aaactgccgg acgcggacaa
    55801 aaaggacaaa aagctcgttc aggcggcggt gtgcgtttag gatttgaagg gggacaaaca
    55861 ccattgttcc gtcgtttacc aaaacgagga ttcacaaacg tgaaccgtaa agactatgca
    55921 gtcattaatc ttgatgtatt gaaccgcttt gaagacggag ctgaagtaac tcctgttgct
    55981 ttagttgaag ctggaatcgt gaaaaacgaa aaagctggaa tcaaagtatt agctaatggg
    56041 gaactaaaca agaaattaac tgtgaaagca gcaaaattct ctaaagcagc tcaagaagca
    56101 attgaagcgg ctggtggatc aatcgaggtg atctaatgtt tagactatta aaagatgctt
    56161 ttaaagtaaa agacattaga tcgaaaatcc tatttactgt atttgtgttg tttgtatttc
    56221 gcctaggtgc acatattact gtaccaggcg taaatgctgc ttcattgcaa aacctttctt
    56281 ctttgccatt tttcaacatg ttgaacttgg taagtggtag tgcgatgcaa aacttttcca
    56341 ttttctcaat gggggtttca ccatacatta ctgcttctat cgtcattcag cttcttcaaa
    56401 tggacatcgt gcctcgattt gttgaatggt cgaaacaagg ggaagttggt cgtaaaaaac
    56461 tgaatcaagc gactcgatat ctgacattag tattggcatt tatccaatct gtcggtatca
    56521 ctgctggatt caatcaatac gctcaattag gtttcgtgaa taatcctaac gtagcaactt
    56581 acgtaacgat cggtttgatt ctaacagccg gtacgatgtt tgttacttgg ctaggagaac
    56641 agatcacaga aaaaggtatc ggaaatggcg tatcaatgat catttttgca ggtatcattt
    56701 ctagattgcc tgaaagtatc agagaaattt atgaagatta ctttataaat attgatcctt
    56761 cagacatatg gaaatctgct atttttgtgg ccattttact agtagcggtc ttgttgatcg
    56821 tgacattcgt aacatttttc caacaagctg aacgcaagat cccgatccaa tatacgaaac
    56881 gcgtagctgg cgcgcctaca agcagctatc tgccgttgaa agtaaatgca gctggagtta
    56941 ttccagttat ctttgctagt tcgtttattg ctacgccaaa tgctgtttta caagcattga
    57001 gcggtagatt cggttctgaa aactggtaca aagtgatgat ggagatcttt aactataata
    57061 cagcacctgg tgctacgatt tatacgattc tgatcgttgc cttcactttc ttctacgcct
    57121 ttgttcaagt aaaccctgag aaattagcgg aaaacttgca aaagcaagga agttatatac
    57181 caagcgtacg ccctggtaag gggactgaag agtatgtctc aagattactg atgcgtttaa
    57241 gtacagttgg tgcagtcttc cttggtttag tggcattgct tccaatcatt gctcaaatgg
    57301 tttgggatct tccacaatca atcggattag gcggtacaag cttactgatc gttatcggtg
    57361 tagctttaga aactgcgaaa cagttagaag gcttaatgct gaaacgtaaa tatgttggat
    57421 tcattgagta agaaagcgtg ttgaggtttc ccctcaacac acgttctcac acatttatta
    57481 ggagggaaaa caatgaacct cattttaatg gggctgccag gcgcaggtaa aggaacacaa
    57541 gctgaaaaaa tcattgatgc ttatggtatt ccgcacatct caacaggaga tatgttccgc
    57601 gcagcgatgc aaaacgaaac agctcttggc ctggaagcaa agtcttatat ggataaaggt
    57661 gcattagttc ctgatgaagt aacaaatgga atcgtgaaag agcgactagc tgaaccagat
    57721 acagagaaag gcttcttatt agatggcttt ccacgtacgc tagatcaagc aaaagctctg
    57781 gacgcaatgc taaaagattt gaataaaaaa attgatgctg tcatcgatat ccatgttggt
    57841 gaagaaatct tggtcgaacg tctagccggc cgatttatct gcagcaattg tggagcaact
    57901 taccataaaa ttttcaaccc tacaaaagtt gaagacacat gtgatcgttg cggtgggcat
    57961 gaattctatc aaagagaaga tgataaacct gagacggtta aaaatcgtct ggctatcaac
    58021 atcaaaaata gtgaaccaat cttggcttat tataaagaac aaggtttgct aaacactatt
    58081 gatggtgctc gtgaaatcga tgctgtattt gcagatgtac aaaaaatcat tgaaaaatag
    58141 cacgattgcc cgtagccgac ttgcattatg cagttactct tgtcaagttc aataaattat
    58201 gatagaatat gtcagtccga cttctagaga atcctctaga tgatccattt tgagaatttt
    58261 gaggtgggaa ctgcagtttc cgccatttta tcgtgtgaaa atgcgaaaca agtacaagga
    58321 ggtactgtgc gtggcaaaag aagatatgat tgaagtcgaa ggtacagtcg tcgaaacttt
    58381 gccgaatgca atgtttaaag ttgaactaga aaacggacac caagttcttg ctactgtttc
    58441 aggtaaaatt cgtatgcatt acatccgtat cttacctggt gacaaagtaa cggttgaatt
    58501 gtctccatac gatcttaacc gtggccgtat tacttatcgc tttaaataat tgtactccgt
    58561 aaaactacag gaggtattat catgaaagta agaccatcag taaaaccaat gtgcgaacat
    58621 tgtaaagtaa ttcgtcgtaa aggacgcgtt atggtgattt gcccagcaaa tccaaaacat
    58681 aaacaacgtc aaggataatt ggaggtgcta taaatatggc tcgtattgca ggagtagata
    58741 ttcctcgtga taaacgtgta gtagtttcac ttacttatat ctatggtatt ggtaacacta
    58801 ctgctaaaaa gatcttagct gatgctggtg tatctgaaga tgttcgcgtt cgtgaattaa
    58861 cgaatgaaca aacagatgct atccgtgcag aagtagataa attaaaagtt gaaggggatc
    58921 ttcgtcgtga agtgaacttg aacatcaaac gtttgatgga aatcggttct taccgtggaa
    58981 tccgtcatcg ccgtggattg cctgttcgtg gacaaaacac gaaaaacaat gcacgtactc
    59041 gtaaaggccc agctcgcgct atcgctggca agaaaaaata attcttaagt gaaggaggtt
    59101 aaaacttcat ggcagcaaaa aaagtgaatc gtaaacgccg tgtgaagaag aatatagaag
    59161 caggtattgc acatatccat tctacattca ataacactat catcatgatc actgatactc
    59221 acggaaatgc attagcgtgg tcatcagctg gttcattagg tttcaaagga agcaaaaaat
    59281 caactccttt tgccgctcaa atggcagcag aaactgctac aaaagcagca atggaacacg
    59341 gattaaagac tgttgaagta acagttaaag gacctggttc tggacgtgaa gcagcaattc
    59401 gttcattaca agctacaggt ttagaagtga ctgcaattcg tgacgtgact ccagttcctc
    59461 ataatggtgc ccgccctcca aaacgccgtc gtgtttaatg agtgccgctc attttgagtt
    59521 ttatcagtaa cgtcattgaa caagacactt ttcgttttga aaggggtaaa gataagaatg
    59581 attgagttcg aaaaaccaag aatcgcaaaa attgatgaag aaaaagatta tggcaagttc
    59641 atcgttgaac ctcttgaaag agggtatggg actactttag ggaattccct acgtcgtatt
    59701 ttattatctt ctttgccagg tgctgccatc actaatattc aaatcgatgg tgtactgcat
    59761 gaattctcta ccatcaaagg tgttagagaa gacgtcactc aaatcatctt gaacattaaa
    59821 ggcttagcgc tgaaaatgta tggccaagaa gaaaaaaccc ttgaaatcga tattaccgga
    59881 cctgctacag taactgctgg cgatattatc gttgatagtg atgtagaaat tcttaacaaa
    59941 gatatgtaca tctgtagtgt ctctgaagga gctacgttcc atgctcgctt aacagtgaaa
    60001 cctggacgtg gttatgttca agcagatgaa aataaaaaag aagatatgcc aattggcgtt
    60061 cttccagtcg attcaatcta cacacctgtt cgtcgtgtga actatcaagt tgagaacaca
    60121 cgtgttggac accgtgaaga ttttgataaa ttgacgatgg agatttggac agatggttca
    60181 attgaaccgt tggaagcaat gagtttgtct gcgaaaatca tgactgaaca tttggatatt
    60241 tttgtgaact taactgatga agcaaaaaat gctgaaatca tggtcgaaaa agaagaaaca
    60301 caaaaagaaa aaatgttgga aatgacgatc gaagagttag acttgtctgt tcgctcttac
    60361 aattgtttga aacgtgcagg aatcaatact gtacaagaac ttacaaataa atctgagcct
    60421 gaaatgatca aagtccgtaa cttaggacgt aaatcattag aagaagttaa agcaaaattg
    60481 cacgacttag gtttaggttt acgtaaagaa gattaaaacc ttttacgaag gagggaaacc
    60541 aacgtgagtt atcgtaaact aggacgcaca tctagccaac gtaaagcgat gttgcgtgat
    60601 ttaacaactg atttattaat caacgaacgt attgtgacta ctgaagctcg tgcgaaagaa
    60661 gttcgctcaa ctgcagaaaa aatgatcact ttaggtaaac gcggagattt gcatgcacgt
    60721 cgtcaagctg cggctttcgt acgtaatgaa gttgccagcg tgcgtgaaga aaatgaggac
    60781 atcgtcattg aatcagcttt acaaaaactg ttcaacgata ttgcacctcg ttacgcagaa
    60841 cgtcaaggcg gatacacacg cattttgaag acagaaccaa gacgcggtga tggtgcacca
    60901 atggttgtct tagaacttgt ctaaaatcac actaataaca tcactttata gatgattctt
    60961 ttagagtgtt atgatgttgg aagatatttt tccgagtcta gctcagcgct gctcctcaag
    61021 cgaatatcgc ttggggagta agcactcatc ataaagtgtt tttttatacc ttcaattacc
    61081 tgtcatgaca gatatgtttg aaggcataaa aaaacatttt aagcgagaaa taattgaaaa
    61141 ttttgtcttt ttttgatagt gttagtagga actttaggat gaagaaggag taatcatgga
    61201 gccaattatt gaattagaaa agataaatta taaataccag ccagatgatc ttcgccccgc
    61261 attgaaagat gtctccttta caattgataa aggtgagtgg atcgctatta ttggacacaa
    61321 tggttcagga aaatcgacac ttgccaaaac gatcaatgga ttgcttctgc cagaatcagg
    61381 gatcgtgaaa gtgggaaatc aaatattgga tgaagaaaat atttggacga tccgacagat
    61441 ggttggaatg atttttcaaa atccagacaa tcagtttgtt ggttcgacag tagaagatga
    61501 cgttgctttt ggtcttgaaa atcaagggat tcccagagaa gaaatgctag tgcgtgtgaa
    61561 agatgcactt gagaaagtta gaatggctga gtttgcttca cgcgagcctg ctcgtttatc
    61621 aggagggcaa aaacaacgtg ttgccattgc cggcgtggtt gccttacgac ctgatatcat
    61681 tattttagat gaagcaacca gtatgctaga tccagaaggt cgtgaagaag tgatctctac
    61741 gatcaagaag atcaaagaag aaagtcaatt gaccgttata tctatcactc atgatattga
    61801 tgaagcagct aatgccaatc ggatactagt gatgagacaa ggcgaacttg tccgtgaagg
    61861 tacaccaaaa gaaatttttt ctgctggtcc tgaactgatc gacttaggtc tagatctacc
    61921 atttcctgaa aaactgaaga gcgcactgaa agaacgtggg gttgatgtgc ccagtgaata
    61981 tatgacagaa gaaaggatgg tggattggtt atggacatcc gttttgaaca agtagacttc
    62041 acttatcagc cgaatacacc atttgaacaa agagccttat ttgacatcaa tatgacgatc
    62101 aaagaaaaca gttatactgc attagtaggg cataccggaa gcgggaaatc aaccttactt
    62161 cagcatttga atgcgcttgt taagccaaca agcggtacag tccatattgg agaacgagat
    62221 attcagccgg atacggataa taagaattta aagcccatcc gaaaaaaagt tggcatcgtc
    62281 tttcagtttc cagaagcaca gctattcgaa gaaacggtag caaaagacat tgctttcggt
    62341 cctaaaaact ttggtgtcag cgaagaagaa gcactagtcc tagcaaaaga aacattagaa
    62401 caagttgggc tggatgaaag ctatttggaa cgttcgccat ttgaactttc aggagggcaa
    62461 atgcgtagag tagcaatcgc tggtgtgctt gccatgaggc cggaagtact tgtattggat
    62521 gaaccaacgg caggacttga tccccagggc agaaaagaaa tgatggagat gttttggcgt
    62581 cttcataagg aacagcagat cactattgta ttagttacgc atttaatgga tgatgtagcc
    62641 aattttgcag attatgtcta tgtgttggaa aagggacgaa tcgttaatag cggggagccg
    62701 caagaggtct ttcaaaacat tgaatggcta aaagaaaaac aactaggcgt acctactgcc
    62761 accgagtttg cagaagaatt aatggctaaa gggatgaact ttgctacctt gcctttgaca
    62821 gctgaagaat tagcagacgc tatcgtccat tatgctggag gaaatgccca tgatgaataa
    62881 attgatattt ggtcgttata tgcctggaga ttccttgatc catcgattgg atccccgagc
    62941 taaattatta gccagttttt attttatcgg aatcattttt ttagcaaata attgggaaac
    63001 gtaccttttg ttggcatttt tcacattact ttctgtcttc ttgtcaaaag tagatttgaa
    63061 attttttatc cgaggcattc gcccattgat ctggctaatc ctcttcactg tagctttgca
    63121 ggtcttgttt acaagtggcg gagaaactta ttggagttgg ggtgttttcc atatcactga
    63181 atttggtgtg atgaacggcc tctttatttt ctgtcgattc gtgttgatca tttttatgtc
    63241 gactctactc acgcttacta caccgccttt ggaattatcg gatgcgattg agtacttgct
    63301 tcgtccattg aaagccgtac ggttcccagt ccacgaaatt tcattgatgc tgtcgattgc
    63361 tcttcgtttt gtgcctacat tgatggacga aacagaaaag atcatgaacg ctcagcgcgc
    63421 tcgcggtgtt gattttggtg aaggaaacct agttcagaaa atgaaagcag tcattccttt
    63481 gctgattcct ttatttgtca gcagcttcaa ccgtgcggaa gatcttgcga ctgctatgga
    63541 agcaagaggc tacaaaggtg gggaaggaag aacgaaatat cgtgtattgc attggcataa
    63601 tagagacacg atggttatca ttgcttttgt attattaact gtgctactga tctttttaag
    63661 aggataaacg ttcagtgggc cgctatctat tcttttcttg ctgatctgtt tgcaagaaag
    63721 gatgcaagca gtgtatgaca agaaatatta gggtctgtgg cagactaagg tcacaggcct
    63781 ctttctttta tctgaaaaga aagaacgaaa aaattttata gaaaaatcat tattccagat
    63841 aaccttttag gaacaaagga gagaaaaaca tggtacgtta caaagcaatc attgcgtacg
    63901 acggaacaaa ttttaatgga ttccaaaaac agccaaatgg gcgtactgtt caagaagaag
    63961 tagaaaaaac attacaaaaa atggcaaatg gaaaagaaat cacggtcttt ggttcgggaa
    64021 ggacagatgc cggagtacat gcgatcgggc aagtaatcca ttttgattat ccagaggagc
    64081 gtccgttaga acgaatgcgt ttcgcgctag atacccagtc accagaggat attgctgtaa
    64141 gacaagtaga aattgtttca gaagacttcc acgcaagata tttggtcaaa gaaaagacgt
    64201 atcaatttcg agtggatata gggaaaccac gcagtccttt tcgacgccat tatgcaagtt
    64261 attttcctta cccacttgat ttggaaaaaa ttcagcgggc gcttcctgat ttattaggga
    64321 ctcacgattt cacctctttt tgtgcatcag gaagctcgat agaggataaa atacgtacga
    64381 tctacgaggc taagatggag gtaaatgaaa caaaagacga attgttgttt acatttaggg
    64441 gaaatggctt cttgtataag atgatacgga tcttagtagg aacgcttttg aaaattggca
    64501 atggccgatt gcctgaagat agtatcccag atattattgc gaaaaaagat cgtaatgcag
    64561 caggacctac agcacaccct gaaggacttt atttgtatga agtgaaatat gaataagagg
    64621 ttatgataga agtggtcagc ttcaagaaat aagaaggtat ttacgaaaat tacttctcaa
    64681 aattttgcaa aatttcggct tattttcgaa gaagctactt ctggaacacc gtttaagaaa
    64741 gaggtcgtga gcctgtcatt taactctgag tcacaaagag caatttcaaa aaacagcttc
    64801 tcatctttga tgaatatcgt gacttgtgga cgaggcgttg ataagtgaat catctttttg
    64861 ttacctattt tttctgtaac caaaaaacga tggaacgttg ttttgattgc ccttggtgcg
    64921 gacagtctga acaggttcca tcgtttcttt attttctacg tcttcttctg tgtgtgtttc
    64981 gtggttgttc ttctattgct ggatcaatcg tgccataaaa ggggcgattg cggtcaaggt
    65041 accagacata aagaataaac aatgcaatac ataggacaaa aaggattgca cctatcgtaa
    65101 atccttcagg caagtaactg aaagtgacag tatgtttacc tgctggaact tttatcatca
    65161 agaatgcgtc tttgaaattg ctgaccttga ctggtttccc atcaatttta gctttccatc
    65221 ctttgtcata aggaatagtt gtcaccaaca tggaatcttc agcggtattt acagtcccag
    65281 aagcttttct gccagtggct tccatttcga cacctttttc tttgatctga ttcattgcgg
    65341 cttgataggc tacaacatct agaccaacga ctttaggctc catgaaactt atttctttcg
    65401 tcccataaaa acttgcagta aatgtcactg ttgtctcttg aggataatag ccaagatcat
    65461 agtattgacc agaaatgttg atttgcgttt ttcgcgatgt accattgaca gtgatcgtcg
    65521 cactagaact ttctaattgc gcaaaattgg ttgggaacaa acttaagtaa gcttgtgtat
    65581 ttgcaggcac atggacttcc caagtgagct cttttggctg gttatctgct gtttctgagt
    65641 aagtgacgcc agtcgttgtc tttgtgatcg tcgtattcaa ctgattcttc aaagtaggtg
    65701 tatagaatgt aaaatactgt tcatttagac cagacaatgt attgatcaag tttgtctgac
    65761 tggtcaaatt atctgtcact ggctgctcaa catcattaac ggacaacggt gcagtaaatg
    65821 ctagtggcaa tgcattgaca ttctgataca gtgtatagtc gccgctttga ccagcttggc
    65881 ggaagccata tttactgaac gtgctttcgt ttttagaaat gttatatttg attcctgtaa
    65941 acccatccat cagcaaagta ttattgttat aacggatatt caaatttgtc cctcttgaac
    66001 ggaaacctaa tttatccagg taagaggaag aattacgatt tcttatagaa gaaaacaaac
    66061 tgataccact gtaaccatag ttgatcgaat cgtttgcaga tactggatcc agattttcca
    66121 agcggtaaaa cgagtcggaa tctttttttg tttcatttac aagagctttc aagtcaggat
    66181 aaggatcagt atataaactg cgggaagcat aattccaatc atcaagaatg ccatggatca
    66241 tactgttcgt attgaccagt gcttcagcag acatgatcaa taataacaat atactcagat
    66301 gattgaatga aagtttttta tgctgataaa atcccacagc aaaagcataa atccctaaga
    66361 aaaccactgt caatacaaag gaagtcatcg tcacataggt gtagcttcct tcaggtttga
    66421 tgccccatgc caccgcaaag agagcagcta cgaccaaaat gattcctaac aacagcccac
    66481 catcgtcttg ttctatcttt tcccatcctt ttcctgcaag caggagcact aagaaagaga
    66541 acagataggc ataacggaat aaaaacatat tcggtgcatg cataccatgc cagaacaaat
    66601 tcaatggaac aagataaaaa cttgcgataa gtatggcgaa aagactgccg tacaaaaatt
    66661 tatttttcaa cggtactttc cgactggcaa aataaaacag acagaggact aatggtagca
    66721 gacctgcgta aacgaacgga atcgatccgt attttgtcgt gtcataaaca ccaatcatat
    66781 ttttcatgat tagatccaga taagaagtcg cttctgtttt caacgtagtg atctgactca
    66841 gtgcctcgcc atttgaccga agatccagca ctgctggcaa tacaatgatc attgaagcgc
    66901 cgcctgccag taaagaagtg ataccataag gaacaatcgt ttttttatat tctgaccagt
    66961 ttgtcaatag acgggcgata aaatataaga aagaaaatac gccaatcata aaacccatat
    67021 aaaaactgga taaaaacaag agtaaataac tgataaataa aagtttcggc cgtttaagat
    67081 ccataacacg atgaatcccc aaaatcacta atggaagata aacaaatgca tccagccaca
    67141 tgatgatctc cgaatgtgca gtgataaatg agatcaatgc gtaactgacg ctaagtgcaa
    67201 catgccccca gcgaggaatc ttgaacgtac gtgcaagaaa ccaaaaagcc aatccggcac
    67261 agccgacctt aagcaaagtc agaacataca gtgcatctgg catcatctgg tttggaaaaa
    67321 agaacaccag tggcgtgaag atcccgccta gatagtaaga aattaaagat aaataattca
    67381 atcctagaga agcattccat gtataaaaaa tactttgctt tccaagaaga acatttcgga
    67441 agctggcatg aaaattagaa aactgtgaaa aggcatcact tgccaaaata cttctgctgc
    67501 ttccaggata gattccgata cttagataaa caagtgctaa aatcaaaaac ggaatcagga
    67561 aactagctgc tgcgtagcgt ccgttttctc taaagaatga ctttaaattt tttttcatat
    67621 ttattttcca ccaaatttct atgaagttgt attcagttta tcatatcctt gtttttctct
    67681 gcttaaaaag agtctagttt tcacaaaaaa ataaaattta gaataaataa cttcataatt
    67741 tgttcgtata acaaaaataa acgcaaaaaa agggtaggaa tcaaaagtcc cacccttaaa
    67801 aatttttatt aaataaaaaa ccgttcacta tctgaacggt ccaagtatca ttcaattaaa
    67861 cgcgttcaac ttttcctgat ttcaaagcac gagttgacac ccaaactttt ttaggttttc
    67921 cgtcgaccat aacgcgaact ttttgtaagt taggtttaac agtacgtttt gttgagttca
    67981 tagcgtgtga acggttgttt ccactttttg tcttgcgacc tgtaaagtaa caaacttttg
    68041 ccatggtaat ttcctccttt gccttgattt ggagcaatag aataattcag ctcataccag
    68101 actaacatat catatagaat gggaatttgc aagtacattt tcatagatac aattgacaat
    68161 tgagaagaat aattgtaaat taaacaaggt tatacctgaa attttgcaag gtttttgagt
    68221 actttgggta actgcctttt atttcaagtt gcgctatgat aaaatatgat agaagaaaaa
    68281 tgggagtttt ttacccgtta aggaggctca ttcatggctg taaaaattca aacgtcagct
    68341 ggaaccattg aaattaccaa tgatgtaatt gcaacagttg ttggtggcgc cgcgacagat
    68401 gtgttcggtg ttgtaggaat ggccagcaaa aatcaaatta aagataatat tactgagatt
    68461 ttgcgtaaag aaaattactc aagaggagta gttgtccgcc aagaagataa tggtattgct
    68521 gtagatgttt atacgattat cagctatggt acgaaaatct ctgaagtttc ccgcaacgtc
    68581 caagaaaaag tgaaatacaa tctagaggca ttattaggcg taactgccaa ttcagttaat
    68641 gtctttgtac aaggtgttcg tgtactgcca gactaggcag gagactggct ggtaactcag
    68701 cagtcgatct gaaggaggat tatctaggtg agtgtaacag aaataagtgc aagccagttc
    68761 caggaaatgg tccaggcagg tgcgaatcgt ctgcaagtaa atgcagaata cgtcaattca
    68821 ttaaatgtat tccctgtgcc agatggcgat acgggaacaa atatgaatct atctatgact
    68881 agtggagcta aggcagtagt agattcaacc tcagaaaaag taggagaatt agcagcaatg
    68941 ctttcaaaag gattgctgat gggtgcgcgc ggaaattccg gcgttatttt gtctcaattg
    69001 tttcgtggat tttctaagca aattccagat gttgtctcat taaatgcttc tgatcttgct
    69061 gcagccttca ctcatggagt tgaaactgcg tataaagcag taatgaaacc agtagaagga
    69121 acgattttga cagtagctag agaagcagcg aaagctggag aaaagaaagc aaaaagtact
    69181 gatgatgtcg ttgaagtaat gacagctgtt gtaaaaggag caaagcgtgc tttagctaaa
    69241 actccagact tgcttccagt cctaaaagaa gtcggtgtcg tagacagtgg agggcaagga
    69301 ctgctgtttg tttatgaggg ctttttaagt gcattgaatg gagaattcca agcggatgaa
    69361 acatatgaac cttctccagc agaaatggat gatatggtca atgcagaaca tcatcgcagt
    69421 gtccaaggtc aaatggcgac agaagatatc cattttggtt actgtacaga aatcatggta
    69481 aaaatcggag aaggacctac agtcgatagt acattcgatt acgaagaatt ccgtaactat
    69541 ttagatggta taggagattc tttacttgtg gtcaatgatg acgagatcat caaagtccac
    69601 gtgcatacag aaaacccagg agaagtcttg aattatggac aaaaattcgg ttcattagtc
    69661 aaaatcaaag tcgacaacat gcgcttgcag catgagacga ttttggaaca cgatgaagaa
    69721 gtgaacgagt ttgcgaaaaa agaaatacca cgtatcccgt atggtatcgt agcgatcgct
    69781 gctggtaaag gcgtccaaga gctgtttgaa agtttaggcg ctaattatgt tatcagtggt
    69841 ggacaaacaa tgaatccaag tacggaagac attgttaaag cgattcaaga agtaaacgca
    69901 gataaagtga ttgtcttgcc aaacaataag aacatcttca tggcagctga tcaagcagca
    69961 gaagtagcag atatcccagt agcagttgtt ccttcaagaa cgatttccca aggattgaca
    70021 gctatgcttt cattcaatga tcaagcttct ttagaagaaa acaaagaaac gatgacagaa
    70081 atgttgtcta gcgttgtgag cggtcaagtc actcacgcga tcagagatac ctcaattgac
    70141 ggtgtagaga tcaaagaagg agactttttg ggcatgatcg atggaaaaat cctgatctct
    70201 aacccagata tcacagcaac agcgttaagt acactagaac aaatgatcaa tgaggacaca
    70261 gagatcgtaa cgatcttggc aggtgatgaa ggaacagttg aacaagcaaa tgaatttgct
    70321 gagaaactaa cagaaaaaca tcctgatttg gaaatcgaga tccaccaagg agatcagcct
    70381 gtctatcctt atctattttc tgctgaataa tttctgtgtc agaatatctt tctaacacag
    70441 tattcattca taaaaggcct tcggttagtg atcaggtatg atcgctgacc aagtcctttt
    70501 tttgttaaag gagggaggaa aaatttatgc gttctttaga tgatccaatc actatgctgg
    70561 caggagtcgg agaaaaaaga gcagctagtc ttgccagctt gggcatccaa acgattgaag
    70621 acttactgac ttattatcct tttcgctacg aagatattca ggaaagaaat ctaaacgaga
    70681 tccaggatca agaaaaggtt actttgaaag gaatcgtagt ctcgcctcct gtgatgaatc
    70741 gatttggtta taaaaaaagc cgattgcaat ttcgtatgat gcaagaacat gatgtgttca
    70801 acgtttcttt ttttaatcaa ccttatctaa aagaaaaagt cattttatcg gaggaaatcg
    70861 ccgtatacgg taaatgggat gcaaagcgta aagccttgaa cgggatgaag attttaggtt
    70921 ctcaaacaat ggatgacttt tcgccgatat atcacgtcaa caaatcaatt cgtcaaacga
    70981 cattagttga tctaatcaaa aaagggtttc aagaatacgg cacattgatt gaagaaaatc
    71041 tgccaaacaa ccttgtggag aaatatcgac tgcttgaccg agcttctgct gtaagaagta
    71101 tgcattttcc aaaagaccac gaagaaaacc atcaagccaa acgacgtgtc gtttttgaag
    71161 agttcttttt atttcaaatg cgtcttcaag gattgaaaaa agcagaaaaa gcagaaacga
    71221 atggtttgga gatcctttat gatgtccaaa aattgaaaca atttactcaa aagctacctt
    71281 ttgaactgac aaatgcacaa aaacgtgtaa cgaatgaaat ctgtcgggat cttcgcagtc
    71341 cgcagcatat gcagcgactt ttgcagggag atgtaggtag cggaaaaaca gtggttgccg
    71401 cgattgcgtt atacgctacc gttacagcag gtttccaagg tgcgttgatg gtgcctacag
    71461 aaatcttggc acaacaacat atggaaagct tgcaacagct gtttgatccc aatgaagtaa
    71521 ccatcgcatt gattacgggg tcaacgaaag caaaagaacg acgtgagctc cttgaaaaac
    71581 tagagcaagg ggaaattaac gtcgtgatcg gcacacatgc attgatccaa gatggagtcg
    71641 aatttcagaa tttaggtcta gttattacgg atgagcagca ccgttttggc gtgaaccagc
    71701 ggaaagtact tcgtgaaaaa ggctggcgac cagatgtctt atttatgaca gcgactccga
    71761 ttcctagaac attagctatt acagcatatg gtgagatgga tgtatctatc attgatgaac
    71821 ttccagctgg acgtatcccg atcgaaacac gctgggtaag aacacctcaa ttggacagtg
    71881 tactggattg gacgaaaaaa gaactggcaa gaggacatca aatgtatgtc atctgtccgc
    71941 tgattgaaga atcagaagca ttagatgtaa aaaatgcagt ggaaatctat gaaaaacttc
    72001 gtgaactatt tgcaccaaaa tatgaagttg gcttgttaca tggtaaaatg aagaatcagg
    72061 aaaaagaagt aatcatggaa gccttcaaag acaaccagat gcagatatta gtttcgacta
    72121 ctgtaattga agtaggggta aacgtgccga acgcgaccgt catgctgatc atggatgcgg
    72181 atcgatttgg attggcacag ctgcaccaac ttcgtggacg tgttggacgc ggaagtgatg
    72241 cttcctactg tatcttagtt gctaatccta aaaatgaact aggtgtggaa cggatgaaaa
    72301 tcatgacaga gaccaccaat ggttttgtac taagcgaaaa agatctggaa ttacgtggtc
    72361 ctggtgaagt atttggcttc cgtcaatctg gtctgcctca atttgcgatt gctgacttgg
    72421 tgacggatgg aaatgttttg gaggtagcca gagaagaggc tgcagccctt tggcaaataa
    72481 aggattggca gatgcttcct gagtatcagc ctttagcgca aagcctccaa atagaaaatc
    72541 aggataatcg ttatttcgat tagcagactg tgctatactt tatactgatg acggaaaaag
    72601 gagtggaaac aatgaaaatt gccgtagatg caatgggcgg agatcacgca cctcaagcaa
    72661 ttgtagaagg cgtggcttta gcgcagcaag attttccaga agtagaattc ttactttacg
    72721 gaaaagaagc agaaatcaag aaatacttaa caaatgaaaa aaacatcacg attatccata
    72781 cagacgaaaa aatcaatagt gatgatgagc cagtaaaagc aattcgacga aaaaagactg
    72841 catcaatggt ccttgctgca caagcagtga aaaacggtga agcagatgct gtattttcag
    72901 cagggaatac aggcgcgcta ctagcagcag ggttgttcat cgtaggacgt atcaaaaata
    72961 tcgaacgccc tggcttgatg tctaccttgc cagtagtagg gaaagaaggt gccggtttcg
    73021 atatgctgga tcttggcgct aacgcagaaa acaaaccaga acatttgttg caatacggta
    73081 ttttaggttc tttttatgca cgtaaagtcc gaggtatcgg acgtcctcgt gtcgcactct
    73141 taaacaacgg aacagaagca acaaaaggta gtgaagtgac gaaaaaagcc tatgaactcc
    73201 ttcagaatga aacaagcttg aattttattg gaaacgtaga agctcgtgag ctactcaatg
    73261 gtgtggctga tgtagtggtc acagatggat ttacaggcaa cgctgttttg aaatctattg
    73321 aaggaacagc aatgaatatg atgaatctat taaaatctgc tattctaaat gaagggatca
    73381 aaggaaaaat gggcgctatg cttttaaaag acggcttacg ttctttgaaa gcagaaatgg
    73441 attattccaa acatggcggt gccgttttgt ttggcttgaa agcaccagtc attaaaactc
    73501 atggagcaac aggccccgat gctgttcgct atacgatccg ccaaatccac acaatgcttg
    73561 agacagatgt agttggacaa ttagtcgaac aatttgaaaa aagcgaagaa tagtatttgg
    73621 atatctgaag cgatccgttc cattgaaaaa aaggtggaca ttcctgaaaa atgccagtaa
    73681 aattagccat ggtgtaggta attgacaagt gtatgattct tggaggtgaa aaagttgact
    73741 cgcgaagaag tatttaacaa agtagcgaaa attatttcaa atcattttga gatagatacc
    73801 gataaagtga cggatgaatt gaacatcaaa gatgatttaa aagctgattc aattagtatt
    73861 atggaattcg ttttagagct tgaagatgaa ttcggtacgg aaatttcaga tgaagacgct
    73921 gaacaaattg aaacagtcgg tggcgcagta gattatatca gcagccatct taaataattt
    73981 caaataaaag caaagaagga t
//
LOCUS       DAZULO010000005        73667 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000005 DAZULO010000000
VERSION     DAZULO010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 73667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.005"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG8OA_000337"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG8OA_000337"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFS4975182.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG8OA_000338"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG8OA_000338"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFS4975183.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG8OA_000339"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG8OA_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFS4975184.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG8OA_000340"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG8OA_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFS4975185.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG8OA_000341"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG8OA_000341"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFS4975186.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG8OA_000342"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG8OA_000342"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFS4975187.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACG8OA_000343"
     CDS             complement(8053..8523)
                     /locus_tag="ACG8OA_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFS4975188.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACG8OA_000344"
     CDS             complement(8569..8994)
                     /locus_tag="ACG8OA_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFS4975189.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACG8OA_000345"
     CDS             complement(9061..9330)
                     /locus_tag="ACG8OA_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFS4975190.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ACG8OA_000346"
     CDS             complement(9341..10936)
                     /locus_tag="ACG8OA_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS4975191.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG8OA_000347"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG8OA_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFS4975192.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG8OA_000348"
     CDS             complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG8OA_000348"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFS4975193.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ACG8OA_000349"
     CDS             15402..16376
                     /locus_tag="ACG8OA_000349"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS4975194.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ACG8OA_000350"
     CDS             16546..17946
                     /locus_tag="ACG8OA_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFS4975195.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ACG8OA_000351"
     CDS             complement(18024..18746)
                     /locus_tag="ACG8OA_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4975196.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ACG8OA_000352"
     CDS             complement(18825..19331)
                     /locus_tag="ACG8OA_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS4975197.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ACG8OA_000353"
     CDS             19454..21253
                     /locus_tag="ACG8OA_000353"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFS4975198.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="ACG8OA_000354"
     CDS             complement(21321..22895)
                     /locus_tag="ACG8OA_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFS4975199.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACG8OA_000355"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACG8OA_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFS4975200.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACG8OA_000356"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACG8OA_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFS4975201.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="ACG8OA_000357"
     CDS             complement(24672..25310)
                     /locus_tag="ACG8OA_000357"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFS4975202.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACG8OA_000358"
     CDS             complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACG8OA_000358"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFS4975203.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACG8OA_000359"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACG8OA_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFS4975204.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACG8OA_000360"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACG8OA_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFS4975205.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="ACG8OA_000361"
     CDS             complement(29104..29844)
                     /locus_tag="ACG8OA_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFS4975206.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACG8OA_000362"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACG8OA_000362"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFS4975207.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACG8OA_000363"
     CDS             complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACG8OA_000363"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFS4975208.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACG8OA_000364"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACG8OA_000364"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS4975209.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACG8OA_000365"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACG8OA_000365"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFS4975210.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACG8OA_000366"
     CDS             complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACG8OA_000366"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS4975211.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACG8OA_000367"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACG8OA_000367"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS4975212.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="ACG8OA_000368"
     CDS             complement(36724..37008)
                     /locus_tag="ACG8OA_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975213.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="ACG8OA_000369"
     CDS             complement(37026..37916)
                     /locus_tag="ACG8OA_000369"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFS4975214.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="ACG8OA_000370"
     CDS             complement(37936..38154)
                     /locus_tag="ACG8OA_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFS4975215.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="ACG8OA_000371"
     CDS             complement(38124..39824)
                     /locus_tag="ACG8OA_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFS4975216.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="ACG8OA_000372"
     CDS             40091..40864
                     /locus_tag="ACG8OA_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975217.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="ACG8OA_000373"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="ACG8OA_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFS4975218.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="ACG8OA_000374"
     CDS             complement(41688..42659)
                     /locus_tag="ACG8OA_000374"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS4975219.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="ACG8OA_000375"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="ACG8OA_000375"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFS4975220.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="ACG8OA_000376"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="ACG8OA_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFS4975221.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45614..46438)
                     /locus_tag="ACG8OA_000377"
     CDS             complement(45614..46438)
                     /locus_tag="ACG8OA_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFS4975222.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46411..47091)
                     /locus_tag="ACG8OA_000378"
     CDS             complement(46411..47091)
                     /locus_tag="ACG8OA_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS4975223.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47114..48052)
                     /locus_tag="ACG8OA_000379"
     CDS             complement(47114..48052)
                     /locus_tag="ACG8OA_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS4975224.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48297..49148)
                     /gene="ispE"
                     /locus_tag="ACG8OA_000380"
     CDS             complement(48297..49148)
                     /gene="ispE"
                     /locus_tag="ACG8OA_000380"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFS4975225.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="ACG8OA_000381"
     CDS             complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="ACG8OA_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS4975226.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52016..52822
                     /gene="proC"
                     /locus_tag="ACG8OA_000382"
     CDS             52016..52822
                     /gene="proC"
                     /locus_tag="ACG8OA_000382"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFS4975227.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(52901..54457)
                     /gene="rny"
                     /locus_tag="ACG8OA_000383"
     CDS             complement(52901..54457)
                     /gene="rny"
                     /locus_tag="ACG8OA_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFS4975228.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(54868..55917)
                     /gene="recA"
                     /locus_tag="ACG8OA_000384"
     CDS             complement(54868..55917)
                     /gene="recA"
                     /locus_tag="ACG8OA_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFS4975229.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56026..57267)
                     /locus_tag="ACG8OA_000385"
     CDS             complement(56026..57267)
                     /locus_tag="ACG8OA_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFS4975230.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="ACG8OA_000386"
     CDS             complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="ACG8OA_000386"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFS4975231.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58294..59217)
                     /locus_tag="ACG8OA_000387"
     CDS             complement(58294..59217)
                     /locus_tag="ACG8OA_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFS4975232.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(59956..61242)
                     /locus_tag="ACG8OA_000388"
     CDS             complement(59956..61242)
                     /locus_tag="ACG8OA_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS4975233.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61235..62503)
                     /locus_tag="ACG8OA_000389"
     CDS             complement(61235..62503)
                     /locus_tag="ACG8OA_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS4975234.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            62755..63216
                     /gene="mscL"
                     /locus_tag="ACG8OA_000390"
     CDS             62755..63216
                     /gene="mscL"
                     /locus_tag="ACG8OA_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFS4975235.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(63276..63722)
                     /locus_tag="ACG8OA_000391"
     CDS             complement(63276..63722)
                     /locus_tag="ACG8OA_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4975236.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(63815..64060)
                     /locus_tag="ACG8OA_000392"
     CDS             complement(63815..64060)
                     /locus_tag="ACG8OA_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFS4975237.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(64175..64753)
                     /locus_tag="ACG8OA_000393"
     CDS             complement(64175..64753)
                     /locus_tag="ACG8OA_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4975238.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(64830..65360)
                     /locus_tag="ACG8OA_000394"
     CDS             complement(64830..65360)
                     /locus_tag="ACG8OA_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFS4975239.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(65364..66224)
                     /gene="rlmB"
                     /locus_tag="ACG8OA_000395"
     CDS             complement(65364..66224)
                     /gene="rlmB"
                     /locus_tag="ACG8OA_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFS4975240.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(66217..66612)
                     /locus_tag="ACG8OA_000396"
     CDS             complement(66217..66612)
                     /locus_tag="ACG8OA_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFS4975241.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(66609..68021)
                     /gene="cysS"
                     /locus_tag="ACG8OA_000397"
     CDS             complement(66609..68021)
                     /gene="cysS"
                     /locus_tag="ACG8OA_000397"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFS4975242.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(68018..68554)
                     /gene="epsC"
                     /locus_tag="ACG8OA_000398"
     CDS             complement(68018..68554)
                     /gene="epsC"
                     /locus_tag="ACG8OA_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFS4975243.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(68640..68879)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(68876..70336)
                     /gene="gltX"
                     /locus_tag="ACG8OA_000399"
     CDS             complement(68876..70336)
                     /gene="gltX"
                     /locus_tag="ACG8OA_000399"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFS4975244.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(70506..71663)
                     /locus_tag="ACG8OA_000400"
     CDS             complement(70506..71663)
                     /locus_tag="ACG8OA_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFS4975245.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(71697..73067)
                     /gene="radA"
                     /locus_tag="ACG8OA_000401"
     CDS             complement(71697..73067)
                     /gene="radA"
                     /locus_tag="ACG8OA_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFS4975246.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(73130..73657)
                     /locus_tag="ACG8OA_000402"
     CDS             complement(73130..73657)
                     /locus_tag="ACG8OA_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFS4975247.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT        20135 a        16074 c        13210 g        24248 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag
    45541 ttcggtattt attaaaataa aaagaagcct ataaaaaatt tttcgttttt ataaggcttc
    45601 ttttctttat aatttagtta ttttttcttc tgaaggcaac caatattcgt ttgatgatat
    45661 ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga ggtgtatcta
    45721 gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa acactgatag
    45781 caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga agaaccatga
    45841 ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc acaccagtaa
    45901 tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg gcggtatctt
    45961 catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata ccgatggcaa
    46021 cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc gaaccaaaca
    46081 gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat actaacgcta
    46141 gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt cggtagatcg
    46201 ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc gtaagtgtag
    46261 gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga gaaagcgtat
    46321 cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg gcgattccgg
    46381 caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc aacgccattg
    46441 agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga tatcttcatg
    46501 atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc ggagaagacg
    46561 ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa gaacaaataa
    46621 gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc cgccagacaa
    46681 ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag cacgctgtac
    46741 atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat atcttcctga
    46801 acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa cttgttgagg
    46861 aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct ttatcattcc
    46921 ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg ctgctccatt
    46981 ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat cttctagtac
    47041 aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca ccttacaacc
    47101 tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt tccatgacag
    47161 aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa ggattcaaca
    47221 cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa gaggcatttt
    47281 cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc tcagctaagc
    47341 gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt
    47401 attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat
    47461 cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata taagcatcgg
    47521 cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct tcagcaatcg
    47581 ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg tattcatgtg
    47641 aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat tctaaacggt
    47701 cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa tcctttaacg
    47761 aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca tcggaaattt
    47821 ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca gggatcaaaa
    47881 gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc ggataaaaag
    47941 tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt ccacatgctg
    48001 caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc atttatatgc
    48061 tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat gtatcaaaat
    48121 aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat attcttgatt
    48181 aaatcgtaaa atggatgagt tgctgctcat atctagccat ttcactacaa taaaaaactg
    48241 aagaatacca ttttaggatt cctcagtttt tcatcaccta ttttacttct tatattttat
    48301 tgcagggttc gtactaaata aacttcctca caaaaacctt ttaacccatt atatactctc
    48361 tgcgctcttg aataatgttg acacaaggca aaaacggttg gtccgcttcc actcatcaat
    48421 gcagcatctg ctccgtactt gatcatacgc tctttgattt gatggatgac tggatgccgc
    48481 tttcccgtta tatcttctaa actgtttccc atttttttga tcatctgctg ataatcttgc
    48541 tctcggatgg cttcagcaag tccgtggatg tcttgatgtt ggatggcttg cgtatctact
    48601 tctctaaaca ctgtacgtgt agagacactc attcttggtt tgaccaacac aacccagcac
    48661 tgtggcatag aaggtaatac ttggatttct tcccctttgc caccgacaaa tgccgtactt
    48721 ccgtagacac agtaaggaac atctgtaccg acttccaaac cgatttctgc cagttcttct
    48781 aacgaacagc ccagattcca taattggttg atccctctta gagcagctgc acagtctgta
    48841 cttcctccgc ctaatccagc tgctactgga atattcttct taacaaagat cttgacaccg
    48901 gttttgatat tgtatttatt ttttagcaat aaagcagctt gataaacatt gttccgttca
    48961 tctaccggta aaaatgcttt atttgtttct acaatgattt tgtcttctgt tatttcttca
    49021 aaaaatagat gatctgccaa gtctacgctc gacatcacca tttccaactc atgataccca
    49081 tcttgccgtc tatataatac atccagtcct aaatttatct tggctggcgc tttttcaatc
    49141 aattccatcc tctcacccat tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa
    49201 ataaaagtat agcttatgtc tgcctagtgg ttgattttgt catcgtttca acagctttca
    49261 cagaattttc tcttcataat ccaaacaagt tctagtgatt cttataattc tgtgagcact
    49321 tcttctgcca aactcggaac atttcctttt tcttttccta tcagactgag tagctgcatg
    49381 gtagcaaatc cgaaattact tcctttccta taagcaaaat aacgtccttc ccaatgatcg
    49441 acatatttta atttcttttc ataagcagac tggaaggaat agaaaaaatt accataatta
    49501 tatacgatat gcatgacttt ttcttgaaag aaagaaaaat ctgtttctcc aacattagct
    49561 aatggagcta agcctagatt ggcttccgag tagcccattt tctcgtatac aataaacaga
    49621 ttggctaaca gatagtcagc taattcctca gattctttag gtaagacccg caacaaatca
    49681 tatcccgcct gcttcttcgt catcggctga gcggtgatga agccaacgac tttttgatct
    49741 ttacggacaa taccgacacc gctagataat acatatgatt tatcaaatcg tcccacagag
    49801 aagtattttt ctcgtgcacc ttgaagccat ttttgtgaaa cttcatttac agcttgatgt
    49861 aattcttccg gcagttgatc gtaccaagta aaatggtatc cttctttcat caaacgttga
    49921 tattcaagtt gctgactcag ccgagacaag tcttctgttg ctagatcaac aatccctgct
    49981 tcacctactt tcgtgaagtg aaaacccaat tcatgaagca tcactacgta gctttctgat
    50041 atcttataaa acgcaagctg atagcctaaa caatctgctt gtttcataaa ctgattcgtt
    50101 gctgcccgca tcattttagg attgccaact ggatctccta aaacaaagca tttgtttcct
    50161 ttgatctgat aaccaaaaac gacttgatct tggtcgtctt cttggtaata aaaataactg
    50221 taacctgaca agcgaagctg atggcttgta gctgtgcctc cgtatgtttc caataaacgg
    50281 tcaaaacggt ctttttcgta ctcactgcca agcttcgtag tatcttcagc taaatattga
    50341 taaagcgcca ctaaagctag caatgagatg cccaaaccaa taagccctga gaaccacaca
    50401 tcatctgaag gaaacaaaat aaaatgattt cctgttattc gaccgactaa aaagcgtccg
    50461 ctgtgatagc ctgcaattgc gtaaacgata aacaaaaagc cgaacaagct agcatcaaag
    50521 attatagctc cccaagaata aacgaatttt gttcgataaa attcttttct ggataaccac
    50581 acagctaaca gaatcaagac atataccaat atcaactgcc aagataacgt acgtgtgatt
    50641 gtatttaaaa taccaaaaga tagtaaagca atcgtaggca gaaatgcctt tttgactttt
    50701 actgaaagtg ctctagcaag gccgagtaat aaaaagccaa ccaatagatt cactgtctga
    50761 tctaaaaaat tgaatgagaa cggcaataat acttgaaata gccgactgat tgcagacaga
    50821 ttagtgattg ttgatagaag taccatcatg atccccgcaa aatagacagc acttacgagc
    50881 acgatatgcg caaccttttg gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt
    50941 ttgactcctg tatgtgtgat gaacaataga agtcccgtca taaaaggtag aacataataa
    51001 aacaaccgat aatataatag ccaaactact gctacatctt gccgaacaga caattgactc
    51061 attcctaaga tcatcaaaac gtcaaaagtc cccattccac ctgggaccat cgtcagcatt
    51121 cctatcaatg tagcaatcat gaacatagga tagatcgaaa gcatcgaaat atctattctc
    51181 atcaacttgc cgatgatcaa aaataccgca agggctcccg tccattgtcc aaaggatgcc
    51241 agtagcagac caagcaagcc ttttggataa aactcgaaga acaatgtctt tctctttaga
    51301 taagcaaaaa agaaaatgac tggtgctaaa aaactaccgc cgaaaagcca gatccaatag
    51361 cttcgaaaaa tactttctga ctgaataaaa aagacatcaa taaatgtaag aaacgaccat
    51421 acagacaaac cagtcagcat aaatagtgcg acttttgata cagtggctaa aaccattttt
    51481 ctatttgtct gctttccata aaaactagcc cgcagacttg ctcctaccac accaccaaaa
    51541 ccggcgagat tgttgatcgt gtttgttacc cacgcagcaa gaagaaactc ttttctttcc
    51601 atttttggtt ttccttgttt ttctaagact ctgatcgtca cccaatcata caacaacatc
    51661 ggagtgacac ccagcagtcc agccagaatc atgagcagga tcgtcatgcg gctttgttgt
    51721 cccatccttt gccaaatatc ttgccaactc atgccattag caatatttgc gacttgattt
    51781 gccacaaaga ctaacacgga gccgaaaaaa atcagtttta aaatcagact atgttccttt
    51841 atccattgca gcatcaagta tcgtttattt ttcacatttt cccattccta acttgtatac
    51901 ttttgtgtca taccccaatg ttatcttatt tattttaatt tttccacttt agacctgttc
    51961 tttgcttgcg atgatagaaa aagaaagata tcctaatgct aaaggagagg ataacatgaa
    52021 agtaggattt atcggcacag ggaatatggc acaagctatt attgatggtt tattaaaaaa
    52081 aggaacagtc tcgaaagaag aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa
    52141 atttgctgct gaaactggcg tccatgcatg caaaagtaat caggaagttg catcacaatc
    52201 agatatcgtt cttttagccg tgaaaccaaa aatcattgaa aaagtattgg aagaactaag
    52261 agaatatagc cagaaagtct tattcatttc tattgcttca ggaaaatcat taatagattt
    52321 agaaacagcg cttgaagatc ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt
    52381 cagcgtagga gcaggcgttt ctgcgatttg caaaaatgaa gctgccacgg aggaacagct
    52441 tcagcaagct acgatgattt ttgaagcaat cggcagcgtc tatcctttag cagaatcaga
    52501 tttcagtaca ttcattggcc ttgctggcag ctcacccgca ttcgtttaca tgctgatcga
    52561 tgcgatggga cggactggtg tactgaatgg tttgtccaaa cagacagcca ctgagattgc
    52621 tgcaaaagca attatgggtt cttgtcaaaa attcctttca agtgataaaa atccatggga
    52681 acttgtagac caagtttctt ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga
    52741 agaagcagga ttcatcccag caatcatcaa aggaatcaat acgacgatcg agaaagatct
    52801 tgagatgttg aataaaaaat aatgatagca atgttttttc gtgccttaca aaaaaagaag
    52861 agctgtggct tttgccgcgg ctcttctttt gtatttttta ttatttagca taatcagttg
    52921 cacgagtttc ccgaataacg attactttga tgtgtccagg ataatccagt tcttcttcga
    52981 ttcgtttacg aatatcacgt actaaacgaa cagcgtctaa atccgagatt tcttctggct
    53041 tgaccattac ccgaacttcg cgtccagctt gaactgcgta acttgattct acaccggcga
    53101 agctgttcga aatattttcc agacttctta aacgacggat gtagttttct aatgattcac
    53161 ttcgcgcacc aggtcttgct gctgagagcg catctgctgc agctacaagg acagagataa
    53221 cagaagtagc ttccacatct ccatgatgag aagcgatcgc gttgattact actgggtttt
    53281 ctttgtactt ggcagctaat tccgcaccga tctcaacgtg agatccttca atctcatggt
    53341 caagtgcttt cccaatatca tggagcaacc cggcacgttt tgctaattgg atatcttcgc
    53401 caagctctgc tgctaagata ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt
    53461 tttgtccata actagttctg aatcgcagac gtccgatgat cttgattaga tctggatgaa
    53521 gtgtatgagc accgacttca aacgctgcct cttccccgta ttcgcgaata cgttcatcca
    53581 tttctttacg agatttctcg accatttctt cgatacgcgc tggatggatt cgtccatctt
    53641 gtatcagttt ttccaaggtc atccgtgcga tttctcttcg gattggatcg aatccggaaa
    53701 gaacaactgc ttccggtgta tcatcgatga ttaaatcgat accagtcagc gtctcaagcg
    53761 ttcgaatatt acgaccttct cgtccaatga tacgaccttt catttcatca ttcggaaggg
    53821 taacgactga aacagtcgtt tctgaaactt gatctgctgc gcagcgttga atcgctaatg
    53881 ataataagtt tttcgctttg cgatctgctt cttctttggc acgctgttca gattctttta
    53941 ccattagagt taactcatga ttcagctctt cttcagtcga tttcatgatg acatctcgtg
    54001 cgtcctcttt tgataaacca gcaatttttt ctagttcttt ttgttgatct tcaatcattt
    54061 tttcaacgtc tttttctcgc tcttcaatta attgctgttt tgaactaagt gcttcttctt
    54121 tactttcaag tgagttctcg cgtttttcta acgagtcatc tttacgattc agcgtttgtt
    54181 cgcgttgaat caaacgattc tcttgagatt ttaattctaa cttactttct ctcagctcac
    54241 tttcaacttc agaacggtat ttctgatttt cttccttagc ttctaacaat gcttcttttt
    54301 ttagagtttc tgcttctttt tttgcactat cgatgattcc agcagctgat gattgtgcac
    54361 cagctatttc tttctcgtga cgtgactttg cgaccataaa ccctaaacca agaccgacaa
    54421 ttaaaccgat gatagcgagg agaatgttga ataccataaa atccacctcc atactatctt
    54481 tttcattttt atcatctata cagcttctat gataaattat tctaaactcg tcaatatgca
    54541 tcttcgcata tatgtgtaca aaaaacatgc acaactttat tctaatgttt gtaccccgta
    54601 gtgtcaactt taaataaaca aagaaacggc tttctactta ttcattaaac gatggttttt
    54661 aaaataaatg acactagttt catataggta aacctttaaa ttgagaaact tttctattaa
    54721 attattcttt gtagatgaac agatattttt attttatgta cccactcgta taattaaata
    54781 actcacgatt tctttattca ataaaaaaac ggcaggtcaa ggagagtacc tactcttcag
    54841 accagccgtt tttgtttatc ataattttta ttcttctact aaaggcaatt cttcttgtcc
    54901 tttttcatct tctgggacag cgacttcttc accaatacca taagctgctc taactagagc
    54961 agaaacttct gccatcattt caggatgatt agccatatag attttagcgt tttcccgtcc
    55021 ttgaccaatc cgatcttctt tataagaata ccaagcaccg cttttatcaa caatgtcttt
    55081 ttctactgcc atgtctagaa gttctccttc ttgtgagatc cctaaaccat acatgacatc
    55141 tacttcagcg attttgaaag gaggtgctac tttgtttttt acgactttga tctttgtacg
    55201 gttaccaaca atatctgtac cttgtttcaa ttgttctgca cgacgtactt ccaaacggat
    55261 cgttgcgtag aatttcaatg cgcgtcctcc aggagtgatt tctggattcc caaacatcac
    55321 gcctactttt tcacggattt ggttgatgaa aatagcgatt gtttttgttt tgttgatcga
    55381 accagagagt ttacgcaatg cttgagacat caaacgtgct tgtaacccga catgtgagtc
    55441 acccatttcg ccgtcgattt ctgctcgtgg aactaaagca gcaactgaat cgactactac
    55501 gatatctaca gccccacttg atactaaagc atcagcgatc tctagacctt gttctcctgt
    55561 gtcaggctgt gaaagaagta gttcatcgat attcacacct aatttttgtg catattgcgg
    55621 atctaacgca tgctcagcat caatgaaagc ggccgttccg ccattttttt gtacttctgc
    55681 aatagcgtgt agtgcaactg ttgttttacc tgaactttca ggtccatata cttcgatgat
    55741 acgtccacgg gggtaaccgc cgacacctaa agcgacatct aacgcaagag agccacttgg
    55801 aattgtagag atttgttggt cgattttttc gcccaatttc ataatagagc ctttaccata
    55861 gctcttttca atttttttca gtgcagcatc taaggctgct ttacgatcat ctgccaatac
    55921 gataatcctc cttatattta caaatcagag caacatttca ttatccttat catatctttt
    55981 ttagtgaaaa aaagcaagaa aaaaccgaac aaatattcgc atttatcatt ttcttaaaag
    56041 ctgttttctc aaaagatcaa atccttgcat aaccgcatta tatctgatag cagcacgatc
    56101 tctggcaaag tgatattttt ccgaaaccac gccgtcttta gatgaaatag cgatccatac
    56161 tgttcctgca gattgacctt ctaacgtgtc cggtccagcc actccggtga aagctacact
    56221 gtaatctgtt cttgctatct cacgtgcttg aatggccatc tgctccacac attctttgct
    56281 gactgttcca tattctgcta atagctccgg atcaatctcc agaaaaccag cttttgtttg
    56341 gagcgagtag gtaacgaatc cgcctggaaa tacttcagat acaccagcaa tgtcgcctaa
    56401 tgcggcttgg aaggcgccgg ctgtcaaact ttccgctgct gttacagtct gcttgttttc
    56461 tttcagtagt tccactacga cttttgctag cgaattatca tcgccgtatc cataaaaata
    56521 ttcccctact cgttcttgga ttttttcttc taatgctaaa agagcttgat tccctgcctg
    56581 aacatcattt gttttcactg tcagtcgtaa tgtcacttca tttggtttgg catatggtgc
    56641 gatcgttgga ttgatctgtg tctcaatcag atcttttagc tcagtgacca gtcgcgattc
    56701 accaatccca taaaagcgca aaactttaga aattagtttt tcttcagaag gaaaacgttc
    56761 ctctagtaaa ggacgtactt gttccacaaa cattggtttc aactcattag gcggaccggg
    56821 aagaaggata tacgcatgtg tatctgtttg ataaaaagtc cctaaagcaa gccctgtacg
    56881 atttggtaga ggaaccccgc cttcgataat ctgggactgt tgcagattgt ttttggtcat
    56941 ttttcgatgg gttgtttcaa aatatgccaa taattttttg tatccttccg tattttgtat
    57001 gagagactta ccgaggtgag ccgcagttgc ttcttttgtc aggtcatctt ctgtcggtcc
    57061 taatccgcca cataagagta tgagttcact tcttgtttca gctagagcaa tcaactcttc
    57121 taaacgctgc tgattatctc ctacaactgt ttgataataa acttcgatac ctagatcagc
    57181 tagctgttct gacaagaaag tggcattcgt attcacgact tgtcctagta aaagttctgt
    57241 tccgacagct attatttctg ctttcattac ttcccgctcc cttatatagt atacgctgta
    57301 tttttatatt tttttattta aaaagagggc aaggagctgt gacaaaaatc ttgtcacagc
    57361 tccttgttcc aaataaacag tgttctcctg ccaaaacctc ggtcataagt cacaatatac
    57421 ataaaacatg aaaagctgtt ttttgaaatt atcccttgtg actcagagtt agacggcagg
    57481 ctcacaacct ctccactaaa cgatacttac attgatcctt tgaacacacc gctgtttttg
    57541 atgaagtaat ccacacctga atagatagta aagatcagac aagcataaag catgatttga
    57601 tctactggaa taccgatcag gctgaatggg atattattga taaacagcaa gataatagca
    57661 accatttgtg tagccgtctt gacttttccc ggccaagctg cagccatgac ttctccatgt
    57721 tcgacaagta gtaaacgcaa gcctgtgacg gctaattcac gacagacaat gatcgctact
    57781 acccaaccag gggcctttcc ttggtcaacc aacatgataa aagcagtcat aactagcatt
    57841 ttatctgcca acgggtccgc aaacttgcca aaattagtga caaggcctcg cgcacgggca
    57901 atttttccat ctaaccagtc tgtgatacta gctaaagcaa aaataactgc acctactagc
    57961 tgtgtgacag ccagtgtggt gtctcctacg ttaaattgtc cccaatctaa aggtaaacct
    58021 acaaccaaaa tgaaaatcgg gatcatacaa attctaagta cagttaattt atttggtaaa
    58081 ttcacactat cgactccttt cttccctatt ctacgtgact atcagcgaag tttccaccga
    58141 tattatttac ttttccaaat gaatttattc aaaggagtat ttttattgaa attgatcttt
    58201 ttgaattaac gaagaaggac cgcgacacag atttgtcacg atccttcttt tttataccta
    58261 caagtaaaca gatgcgtaaa ctaatttata cgatcattct gcgtattgaa gagtaaggtt
    58321 cacatttttt ggactctgat tgttttcccc tggattaact ggtacggatt ctccattcgc
    58381 tttgatcgtt gcattgcttg ctgcaccaat cgtgatggtc gcatttgttg ctgtttccgg
    58441 taacacggta ctctgtgttt catttgctgc cagtgtccct tgataaacat aggcattgtc
    58501 tactagcaca cctaaccata cacgattgac agcagtaaat tcaagggtta caggcttggt
    58561 agcgtctgtg atatttactg cgatggcact gccagaatct tctcctgtag ctattgccat
    58621 tttcttttct tcacttgatt ttgttgttga ttctactgta gaagactctt tggtctgcgt
    58681 gctttgctct gaggaagagg actcctcgac tgatccttct actttaagcg aagaagctgt
    58741 gtctccgatt attggatcag cttgacgatc ttgccaagtc atatagccca cgacaacgac
    58801 aatcgccaaa gcaaccaatc ctaataaaat gacaggaaga cttgtccaaa agcgacttgg
    58861 atgcgattct tcctgatgca ttgcttttct agaagtactt actgtttcag gctgcggacg
    58921 ttcagggacc gatcctgtca atttgctttt cccatcaaaa acatcgacta aatggtctcc
    58981 atcttctcct acagcttcag cgtattgccg aataaatgcc cgcacgtaaa atttaccagg
    59041 caattggtca aaatcatcat tgtcgattgc ttccaaataa cgtttttgaa tttttgtaat
    59101 ctgctgtaat tcatctaaag atattttttt attcaggcgt gcttgccgta atttttctcc
    59161 gatggtttca aaagccactc gttcatcttc tccagtctgt ttgattctcc gaagcatctg
    59221 ctgttgcaac gaaagacgtt atgtttgggt gctgtgacaa aagtcatgtc acagctcctt
    59281 ttttccgaat aaacattgtt ctcctgccaa ctcctcggtc acaagtcaca atatccaata
    59341 aatataagaa gctgtttttt gaacttgttc cttgtgactc ggagttaaac ggcaggctca
    59401 cgacctcttt cttaaacggt gttcaatcag ctgttcctcg gtattagctc aaaaaccggc
    59461 aaaatataag gagatatttt tccgtatgtt tctctaatac tcaggtcaaa acgctgattt
    59521 cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa
    59581 gttaaaaaca ttaagaagct gtttttactt ggctgttctt attacttgat gcagggcagc
    59641 tttttcacaa cctcttggta cacggtgttc cagaagtagc ttcttcggca ataagccgaa
    59701 atatcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
    59761 acttctgtca caacctcttt gattacttcg tttgatttta ttttaatata agttgattaa
    59821 aagagagcat gatttgtttt attttctcac taatttttcc aacacaacta agaataccat
    59881 aaaagctgat tttggaggag gttcctacta caagtctaaa acgaaattta agacttttcc
    59941 tcataaaata ctggtttatt ttggtctcat gtaaaactcg ctgaaggcct cttcacttat
    60001 aaatgcttcc ccagctgcca gtacgtcttt catttggatc gaatcgatga tttcaggaag
    60061 atcaaataaa gttcgatcgc caaataaact ttgcgtaaat tgattggcaa tatattcgat
    60121 cgaatttaat gattggaaat attgtccaag cattttcttc ttcagcaaat cgagatttgt
    60181 ttctgacact tccggatcat cagcgaatcc aagaatgatt tctttgactt tttctgctgc
    60241 tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag tgaaattcac gatccaagct
    60301 gaattcaaaa ccaaagctgt catcgataat tccttgattg tacatcgcta gatagtttcg
    60361 agaagtattg cctaaaatca tttggaacaa cagattgatt gctgttttat agcgtaacag
    60421 ttctgttcct tcttgcggca atgtgtctaa tcctttaata ccaagtacaa atttatctcg
    60481 tgtaatggct gcttccaatg cactttgttt gatgatttct tttgtgtttt cagggaaata
    60541 acgtacaatt tcttgctttg gagggaaatt ttttgcttct tggttttccc gaataagttt
    60601 catcaatttc tcaggctcca tcttaccaac tacaaataaa accatattgc ttggctgata
    60661 aaacgtccga tagcacgtat acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt
    60721 gcccgcaata tcgatatgca atgggtgtgt aggatacaag ttatttagta tgccaaaaaa
    60781 catccgccag ttagggtcgt cttcatacat ttggatttcc tgaccgatga ttcctttttc
    60841 cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag
    60901 attcttttct acctgatccg tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa
    60961 agcattggcg gaagctcctt gtttcccaaa taattgaaag acatcaccat cttctttttc
    61021 aaacaactta tgttctaaaa aatgggcaat tccatctgga actttcacct tttctttttc
    61081 accataggga ataaattcgt tatcgatcga accataattc gtactgaaca aaccatatgt
    61141 cttgtgatag tcatttttag gcaacaaata cacggtcaaa ccgtttggca gtacttcatg
    61201 ataaagtgtt tcattgattt gctcatattc tgttttattc atttgcattt cctcctgcta
    61261 aaaagaagat tgcctgcaat tcgatttgct cagctacttg ttgtatctct ttgatcgtta
    61321 cgctctccat acgtttcaac cattcttcat cagataattt tgtttccggc agccagctgt
    61381 ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag agaaagcaga tattggtttc
    61441 tcagcattgc ttttgtctga gacagttcca actctgttat ttcaccattt cgcaaacttt
    61501 ctaattgctc gtgaatcaga tgaagtactt gatttcggtt cttttcatcg ataccggttt
    61561 ggacactcat gaatcctcgg aacgtatcca cgctactaga ggcataataa gctagacttt
    61621 ctttctctct gacattcatg aaaagtttag agtgaggaaa accgccaaat aaaccgttaa
    61681 agaccattag cgcaaaacgt tctggttcat cgtaatacac atttgtctga taagctaggt
    61741 tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc cttcacgata ttcacttgag
    61801 gttgggtata aaacagatct ggataaactg tttcccgcga ctcaaatggt aattgcccaa
    61861 ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac aaaaatgtcg atttgatcat
    61921 ttgccatcat ttcttgatag taggacgcta gcgaactagc agtgatcttg tccaaatctt
    61981 cgactacgcc caaccctggg atcttttgtt ccgggctgtt ttcaaaataa agttcttgca
    62041 aggcatagga agcaaaagtt tgtttatctt ccttcaaact ttccaaatat agacgcagat
    62101 tgttcttttc caagtcaaac gtcaactgat caaactgatg atttttaata tttggataaa
    62161 ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag gtttggatca ttgacatatt
    62221 caccattcac aaaactgatc cctgcgttta gccaatgtaa atttcctttt tttctaactg
    62281 ataatccaaa gcttgcgcca taaagctctg caagttgcgc acttaacttt gtctgatccg
    62341 aataattcaa actatttgtt tccaacatgc tgctgagaag tgaacgtttc gtgatgactt
    62401 cttgttgcag acgcgcagta aaacggataa aaatccggat cgttttgtat tgttttgttg
    62461 gtaagacatg aagattcacg ccttctgcca attgatacac catatttata tgctcctttt
    62521 cacagttgat tgcccgctta ctcttttaat gggtcaacct gcctttttta cttattcctc
    62581 tgcttgaaat ttataagttc aggcaattcg ccgttttttc taaatgatgt tacactttat
    62641 agtatagcat agatgttttt tatcaaaaga aacagacggc taaaagcgta tattgttttt
    62701 ttatttttta tttttcgata tactaaagac aaataaaaga agggagggtc attcatgatc
    62761 aaagaattca aagagttcat catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt
    62821 attggtagtg cgtttacaag catcgtaaac caagtggtgc aaggcttgat cacaccacta
    62881 ataggttggt ttgtcgcaat gatcacaggt acttctgatt tagaaggagc actttcaatt
    62941 cttgactggt cgccgacaaa aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc
    63001 attacatttc tgatcactgg ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg
    63061 aagaatttac gctccaaaga agagcaagtg gaagtggaag aaactcctac tgcagaagat
    63121 tatcttagtg acatacgcga tcttcttgcc aaacaagtgg cagaagacaa tacacatcca
    63181 gtagagcaaa caaatctttc cgatacttct aaataataaa gaatctaaaa aaaaagaaag
    63241 ccgattttca gtgtcggctt tcttttttta gattcttaac ttgctacttg ttcctttttc
    63301 atgtcgatat gtggaatatc atcttccaaa tacacatctg agattgcctt gaatccaaac
    63361 gactcataaa aagcttgtag gtattcttgt gcgccgatca cgattggttt ttctggaaaa
    63421 tttttctgga tcatgttaat cgtttcttga acaatctttc ttcctaaacc atctttacgg
    63481 ttgtcctgat gaacaaggac acgtccaaaa tggatactct ccgcttcttc atatactcgc
    63541 gtgtaggcaa ggatcttccc atcagtatct tgaaaaaacg tatgccaagc ttctccatca
    63601 atgtcatcaa cttcttgata aggacactgt tgttccacaa caaaaactgc gattcgttct
    63661 ttgacgatcg cgtaaaactc tgctgttgac aattcttcaa atttctttac ctgatattcc
    63721 actaagcaaa cgccccctgc ttttttattc ttagcatacc gattcgcaca caaaaaagca
    63781 aacgaacctt acgaatctca ggctcgtttg ctttttaaac tgcttcagtt aaaaattcga
    63841 tctcaactgt ccgagtaagt acatctgaat agctgtaaga tactcgttca aaagaatttt
    63901 cttcaggatc caaatcaaca acgaatactg aaggataggt ttctgttaaa acgccgcggc
    63961 gttctgtttg acgctttctt cctgtctgag caaccaacat gattgggctg ccgatacgac
    64021 cttctaaatc tttcttgatt gaagctaaag ttgttggcat acagttcacc tctactatca
    64081 actataccac atatttttaa aattttcaag tttaccataa aaaaaaaaga aatgcaataa
    64141 aaataaataa gaagctcatt aagatttttc taaattattc ctcattataa aatttcaagt
    64201 gtttttttat tttgagtttt actctcccgt aagcgctcat tacttgcttg tgatccattg
    64261 ctaaacattc agccatgtct acaaaatcaa cgccttgcat ataataatga aaaattttag
    64321 cttctatttt tgaaaactgg atttcgcaat tttcgattgc ttcttttaat aaaagatgct
    64381 gaattgctaa aggttcttgc aatgtgatct ctgctaaaaa gtctggtcct tctgtctcta
    64441 ttttttggtc gattgagact tcatcttttt gatcacgacg cttcagcgcg ttctgcttcc
    64501 tcaacagaga atagatttga ttgcttataa cgcttttata ataacttcca aagctaacac
    64561 ctttgctttt ttcgtaagtt ctcaatgcct gatataaagc gatccttgct tcttgttccc
    64621 aatcctcaga aacataattt cttaaataat aggtgttttt caaacgtagt aaaagtggaa
    64681 aatatttttg atacagggca gcaaatgctt cttcgtcacc attacgtgca gcgcgcaata
    64741 actcctcaga cataaaataa ttcctccata aattcgttta tggaggaatc ttatcatcaa
    64801 tgattcatcg aactgattag aacgatatgt tatttatttt ttctcattaa atcgtccaat
    64861 ttttgggaca atttttttaa ctgctcttca ttccatggag aattacgtcg gaaatcaata
    64921 aaatgcatat ctttattttc ttgggctatt gttttttccg ttttttttat cgtcttatat
    64981 aattcataag cagaagttcg taatgctcct tgggaaaaga tcatccactg ttctgctaaa
    65041 tcgcttgtcg ccactgtcac ttgtgtcaaa cggtcattca gctgaccagc gattctctcg
    65101 atataactgt cagccgtttc gtcttctttc gtaaaaacaa catccaactg gtatttctta
    65161 taattttgtt ggatgcccgg aactagttgg gcatcgaaga caacgatgac ttccattcct
    65221 tcatatttcg cataatttga taaacgatgt aaaagagctt ctcttgcatc cgccattttg
    65281 tcctgctttt taagctggac aagttccggc cacgaaccaa tcatgttgta gccgtctaca
    65341 atcaacagct gttttttcat ggtctactcc ccctgacgtt gggaaaaagc ctgatacata
    65401 agcaaaccag cagcaacact ggcatttaag ctctggacgt gcccggtcat cgggatcgtc
    65461 agcaattcat ccatttcttt tttcaatcct ggactcattc ctttgccttc attaccaatg
    65521 actaaagcaa ttgatccttt ggcattccat ttttggaagt ttgtgccggt catatctgtg
    65581 ccaaataccc aaaatcctgt ttctttgagc tgttgcacag cttgtaccaa attcgtcacg
    65641 cgtgcaacag ggacatgttc gactgcccca gtggaagtct ttgttacgat aggtgtgatt
    65701 ccaactgctc ggtgttttgg tatgatcaca ccatcgacgc cacttgcgtc tgctgtccgt
    65761 aaaatcgacc caaaattgtg tggatcttcg atgctatcca aaatcaaaaa gaaggggtct
    65821 tcttttttct cttttgttcg ttcaatcagt tggtcaaggg ataaatattc ataaggtgta
    65881 atcgcaagaa cgactccttg atgcacgccg tgatcgctca aagtttctaa tttttgttta
    65941 ggaacccatt tgactggcac agcttgttca cgtgcgattt ctttcagttc ttctactttt
    66001 tctcctcgtg aatcttcttg aagaaagagt ttattccctc gcccttgttg tagcgcttct
    66061 actgcggcat ggtgcccaaa gacgaaatta tcttctaatg cttcttcatt ttgatcagaa
    66121 actgactgat ttcgtccagt ttgcttttta tttgaatatc tattttgttt tttgtcttca
    66181 aatccacgtt tcgattgatg tttgtttgtt ctttctttac ccattttttt caccaacttt
    66241 ctgaatacac cagcggatca attcttccat ccgctcggtt tgttttgtca aatgcagata
    66301 gcccatgagc gcttcgaacc ctgttgcgac ccgatacgtc gtaacatcag cattttttgc
    66361 gctcgtatgg cttttagcat ttcttcctct gcgataataa agttcttctt cttctgtcaa
    66421 aagatgttct tccagcattt gctgaatcaa aaaggcttgt gcattcgcag aaacataatt
    66481 cgttgccgca cgatgcagct tattgggttt tgtttctcct tgatcaatca ggtggtcacg
    66541 aacatagatt tcgtaaatcg catctccaac ataagccaag gctaaaccgt tcaattgtgt
    66601 gtaatctctc atgcttctct tctccatcgg gttccttgcg gtgtatcttc taataagatc
    66661 cctttttctt tcaacaaatc acgaatttcg tcgcttcgga caaaatcacg attgcttctt
    66721 gcttgatttc gttcttcgat caattgttca atctcttcat ctaataactc ttctgctgta
    66781 aataaaatac cgaagatcga aagccattgc gagaataaag cttctccttg tgccaagaca
    66841 gcggcagaaa ctttcggctg gtctagataa gtgttcagcc acttagcaag ctcatagacc
    66901 actgtgatcc catttgctgc attaaaatca tcgtccattt cttcgatgaa tcgctgttct
    66961 aaattttcta acaaagcgat tttatccgca tcatctgcca aattctctga ggcagtttct
    67021 tggcggaatt tcaaattgtc aaacgtattc ttcagtcgtt gtaaattggt ggctgcttct
    67081 ttcatcgtcg tttcactgta gcggatcgga cgacggtatt gtgtggtcgc catgaagaaa
    67141 cgcaagactt gtggatcaac gtttttgatc atttcatgta cagtgatgaa attccctaga
    67201 gatttactca ttttttcatc gtcttcgcca atcgttacat agccattgtg catccagtaa
    67261 ttagcgaatt ttttccctgt tttcgcttca ctttgtgcga tttcattttc atgatgagga
    67321 aattctaaat cttgcccgcc accatggata tcgatcgttt ctcctaaatg ttttgttgcc
    67381 ataacggaac attcaatatg ccaaccggga cgtcctttgc cccatggtga atcccaagaa
    67441 atctcgccag gttttgcttg tttccataaa gcgaaatcta atgggtcttc tttttgctgc
    67501 tgttcttccc ctgtacgctg acttgctccg acttccagtt catcgatgga ttggtcactt
    67561 aatttcccat aattttcaaa tttacgggta cggtaataca catcacctga agattcataa
    67621 gcatagcctt tttcaaccaa cacttgaata aatgctaaaa tatctggcat atgatccatg
    67681 actcttggat gcagtgtagc aggttcgaca ttcaacgctt tcgtatcttc ttcaaatgct
    67741 cggatgaaac gatcagcaac ttctggtgct gtaataccta attcgtttgc tgtacggatg
    67801 attttatcgt ctacatccgt gaaattcgat acgaaatcga ctttatagcc tcgatattca
    67861 aaatagcgtc gaattgtatc gaaagcaatg gtgctacgag catttccgat atggatataa
    67921 ttatacacag tcggtccgca gacatacata ccgacatgtc ccttttgtat agggatgaat
    67981 tcttcttttt ttctggtcaa tgtattgtag atttttatca ttctgatctc ctcacttttt
    68041 ctcctttgat tcgaacgact tttgctggta ctcctaccgc tgtagcgtcg tctggaacat
    68101 ctttcaacac aacggcacct gcaccgattt tggcgttttt tccgatcgtt accggtccta
    68161 atatctgcgc attcgcagag agcatggctc cttgtttaac agttgggtgt cttttacccg
    68221 tttcttttcc tgtgccgcct aatgttacgc catggaataa aatcacatcg tcctcgattt
    68281 ctgcggtttc gccaatgacg attcccattc catggtcgat gaacacgcca gttccaatcg
    68341 ttgctcctgg atggatctca acaccagtta ggaaacgcca gaattgagca ttgatcttcg
    68401 ccaataaaaa gagccggtgc tgatataaaa aatgagagaa acgatgccaa aacaaggcat
    68461 gaaaaccagg gtatgtcaaa acgatctcag ctgtcgaacg tgcagccgga tcgtttctct
    68521 ttgctgcatc aatcgctcgg gtaaaccaac tcatgacttt ccactccttt cgtaagaatt
    68581 ttatgtagga aatcatcttc ttataaagaa aagaagcgcc ttttaaataa gtttccttat
    68641 caaaagacgc tttccgcgtg gttccacttt tcttcataat caatgattat ctctgacagc
    68701 taacgcctgt tatacgttct tggctacttt gtttcctata aaacagtttt tcctgttttc
    68761 tgtgtttcgc caagaccact cccagaggca tttcggcaac cattcattga taccttgcac
    68821 cacccggtat ctctcttgta atgatcagct gcttactttt ttctgttcat cgtgtttagt
    68881 tcagtacttg atttaaatgt gctaatgatt tttcacgacc aagcaattcg atcgtttcag
    68941 gcaattctgg accatgcatt tgtcctgata cagctacacg gattggcatg aaaagatttt
    69001 ttccttttac gccagtttct ttttgaacag ctttgatggc agctttgaca gttggagcat
    69061 ctactacttc catttgttct agctgtgctt tgaatgcgtg gagaacagta ggaactgttt
    69121 caccagcaag tacttctttt gctgcttcat tcaatacagg atgttcattg aaaaatagcg
    69181 tagacagatc aacgatttct gcagcatagc tcatttgagg ttgatacagg ctaacgatct
    69241 tcttcaccca ttcgattgtc ttttcatctg ggttttctgc tacacgtcca tcagcgatta
    69301 gataaggcag acacatgtca gtaaatgcat ctagatccat tgctttgatg taatggttgt
    69361 ttacccattg cagttttttc gcatcgaaag ctgcgggtga tttacttagg cgttttggat
    69421 cgaacatttt gatcaattct tggtgttcga agatttcgtc ttcgcccaca ggtgaccagc
    69481 ccaataatgc aataaagttg aacattgcat ctggtagata gcctaattcg cgatattgtt
    69541 cgataaattg caagattgat tcgtcacgtt tgcttagttt tttacctgtt tcactgttga
    69601 tgatcaatgt catatgtccg aaggttggtg gtgtccagcc aaatgcttca tagatcatca
    69661 gttgttttgg tgtattggca atatggtcat ctccacgtaa aacgtgagtg attttcatca
    69721 aatgatcatc gaccgcaaca gcaaaattat aagttggcat accatcgcgt ttttggatga
    69781 caaagtcgcc accgatatta tcggattcga acgaaatagc gcctttcacc atatctgtaa
    69841 atgcgtattc tgtattgcga ggcacacgga aacgtacaac agattctaga ccttgtgctt
    69901 ctttttctgc ttgttcttca ggtgtcaagt tcgcacatgt tcctgcgtaa tgaggcattt
    69961 ctcctcttgc gcgttgtgct tcccgttctg cttctaattc ttcttctgtg caatagcatt
    70021 tgtatgctcg attgctagca agcagctgat caatcagcgg ttgatagatt tctttacgtt
    70081 cagattgacg gtaagggccg tattctccag gattttcagg agattcatcc caattcattc
    70141 ccaaccaagc aaggttttcc aactggcttt tttcgccatc ttcgatatta cgtttttggt
    70201 cagtatcctc aatacgaata ataaagtctc catcattgtg gcgggcaaac aaataattga
    70261 ataatgcagt tcttgcattt ccaatatgca ggtgcccagt tggacttggt gcatagcgta
    70321 cgcgtacttt cgtcattttc ttcctcttct ttcaaactag actttgacta acagcaaaaa
    70381 ttccgtcagt tacggcttct caaatatcat ttatttaaag ccactcagca caaattttac
    70441 cttttctaag gtaaatagta aagccaactg gttgaatttc cttaccagtt ggctgaattt
    70501 cttttttatt ttgattgttt agtctttttc tctgtctctt ttttattgtc gattcctcgt
    70561 cctgcatgtg caggtttagc aaagatcatg cgtccagcag ctgtttgaag ggcacttgtt
    70621 acaacgactt cgatacgttc attcatgtaa tgctgtccat cttctacgac aaccatcgtg
    70681 ccgtcatcaa ggtaggctac tccttgttgg cgttctgtcc cggcttttac tactagaaca
    70741 ttcatcgttt caccagggat gacgactggt tttacggcat ttgccaacgc attgatatta
    70801 agaacaggta cattttggaa ttcagagact ttattcaagt tatagtcatt ggttacaatg
    70861 acaccatcta aaagcttagc caatttgatc aatttgctgt ctacttcact gatatcttca
    70921 aagtctccat cgtacatttc gacagatata ccctcttctt tttgcaaggc atttaggata
    70981 tctaatccgc gacgaccacg cacacgtttc aaactatccc ctgagtccgc gatgtattgt
    71041 agttcgtaaa gaacaaaatt agggatcatc aaggttcctt ctaagaatcc agtctttgta
    71101 atatcataaa tccgaccgtc aatgatcacg cttgtatcca agattttata tttatggaaa
    71161 ttttctcctg attttcgttc taacagttga ctctcaactt gactagctgt ttcttgctcg
    71221 atttttttgc ttcgtgaacc aaaaactttc ttccattctt cgattcgtgt cgttcccatt
    71281 ctcaatccca gatagcctaa gatgatcatc agtaaaatcg gcaatacact attgacgaat
    71341 ggaatttcca tattgtaaag aggggtcgag atcacaacac ctaaagtcaa tccgatgatc
    71401 gcaccgatcg caccgaagag aagataagtc aagctgacct cacttagttt ttgttctact
    71461 tgtttgataa tagctgagat atgtttggcc aacagcaaag ataataaaaa gaaaataagt
    71521 gcgcctaaca ggctgttggt aaatgtatta ttcagccatc cgtttgactg ttgaccgatt
    71581 gcctgccaag ctgagggtaa aaatgaaata cccaagcttg cgccgacaat gatcatcagc
    71641 aaagtaatga cacgtttttg catcgttttt cctccgtttc gacaatcatt tattagttag
    71701 cggaacacac gcttcaacgt ttcagccaaa gttgctactc caactatctc gattcctttt
    71761 ggcgcttccc agttacctaa gttgttttta ggtaggtata cttttgtgaa gccgagtttt
    71821 tggacttctt tgactcgttg ttcaatatga ctgacacgtc tgatttctcc agtcagtcca
    71881 atttcaccga tgaaacattc cgttggctgt gttccttttt ctttatagct tgaagcaata
    71941 ctgacagcga tagctaaatc aatcgctggt tcatttaatt ttaccccacc tgcagctttt
    72001 aagtaagcat cttgattctg aagaagcaac ccagctcttt tttccagcac tgccatgatc
    72061 agagataccc gattgaaatc aagacctgtt gttgttcgtt tggcatttcc gaacatagta
    72121 ggtgtgacta atgcttggat ctctaccaaa attggacgtg tcccttccat tgccacaacg
    72181 atagcagagc cagttgcacc gtctaggcgt tcttctaaga aaatctgtga aggattagcg
    72241 acttcttcca atccatgctc atgcatttca aaaatcccaa tctcattcgt tgagccaaat
    72301 cggtttttta ctgctcgcaa aatccgaaaa ctatgatgtt gttcaccttc aaaatataac
    72361 accgtatcca ccatatgttc taacattctt gggccggcga tcgagccttc tttggtaaca
    72421 tgaccgacga taaaaatggc tatcccattt gttttagcgg ttttgagtag ttctgcagtg
    72481 gtttctctta cttggctgac acttcctgcc acacttgtga tgtctggctg cgtcatcgtt
    72541 tggatggaat caatgataac ataatcaggg gagatatgtt cgatggcacg actgatttcg
    72601 ttcatatctg tttcagcgta tagataaaat tctgtgttga tacttccgag gcgttccgcg
    72661 cgtaatttta tctgctctgc gctttcttcg ccggatacat ataagacttt tccgcctatg
    72721 gctgctaatt gctgagaaac ttgtaacagc aaagtggatt tcccgatacc tggatcgcca
    72781 ccaatcaaga ccatagaccc tggcaccaca cctccgccta acacacggtt cagttcttct
    72841 aattttgttt tgatccgagg ttctttttta gggacgacgt ctgcgatttt tgttggtttt
    72901 gctttttctc ctgtcaaact tgtccgagtt ctgcggtccg ttgtatcttg ttcgatttct
    72961 tcgaccatcg tattccattt tccgcaattt gggcagcgtc ccaagaactt aggcgacaca
    73021 tacccgcaat tttggcaaac aaattgtact tttgattttt ttgccattgt accgcctcct
    73081 tttatcctaa tcatgagaca ttaaatcaag cttaagtatc tttaacgttt tatttactgg
    73141 aagaaccaaa tccgccttga cgttgagcag tcgtttgatc ttgatctgct tttaaaaacg
    73201 gcaaaaagat cccttggccg atacgctcgc cttttttgat cactacatcc ttgaaaccaa
    73261 agttggtgaa ttggaacatg atatggcctt catttgcctc attattgtaa taatcactgt
    73321 caatcacacc tactccgttt gtcaacacta aaaaccgctt caacggattg cttgaacggt
    73381 tagcaagctg caaaaattca tcttctccca tgtaagcttt gattccagta ggcacgaggg
    73441 ttggtttcaa ttgtttttca ttttctgata catctttttt caatccatct gcgactgttt
    73501 ccaacccctt tttccagcct gctttccaga tacttggaat cacgacctct tctgctgctt
    73561 caaaatcata gccagcagca tgatgtgtag cgcgctgcgg tagattgata ttttgatctt
    73621 tatatttaga aataacttca aatcctcttt ttctcaccgg ttttcct
//
LOCUS       DAZULO010000006        68384 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000006 DAZULO010000000
VERSION     DAZULO010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.006"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            40..921
                     /locus_tag="ACG8OA_000403"
     CDS             40..921
                     /locus_tag="ACG8OA_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4975248.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            934..2544
                     /locus_tag="ACG8OA_000404"
     CDS             934..2544
                     /locus_tag="ACG8OA_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4975249.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2621..3280)
                     /locus_tag="ACG8OA_000405"
     CDS             complement(2621..3280)
                     /locus_tag="ACG8OA_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4975250.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3563..4228)
                     /locus_tag="ACG8OA_000406"
     CDS             complement(3563..4228)
                     /locus_tag="ACG8OA_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFS4975251.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4375..4980)
                     /gene="dhaL"
                     /locus_tag="ACG8OA_000407"
     CDS             complement(4375..4980)
                     /gene="dhaL"
                     /locus_tag="ACG8OA_000407"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFS4975252.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5008..6018)
                     /gene="dhaK"
                     /locus_tag="ACG8OA_000408"
     CDS             complement(5008..6018)
                     /gene="dhaK"
                     /locus_tag="ACG8OA_000408"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFS4975253.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5993..6388)
                     /gene="dhaM"
                     /locus_tag="ACG8OA_000409"
     CDS             complement(5993..6388)
                     /gene="dhaM"
                     /locus_tag="ACG8OA_000409"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFS4975254.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6542..6862
                     /locus_tag="ACG8OA_000410"
     CDS             6542..6862
                     /locus_tag="ACG8OA_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFS4975255.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6974..7831)
                     /locus_tag="ACG8OA_000411"
     CDS             complement(6974..7831)
                     /locus_tag="ACG8OA_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS4975256.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(7939..9242)
                     /locus_tag="ACG8OA_000412"
                     /pseudo
     CDS             complement(7939..9242)
                     /locus_tag="ACG8OA_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(9239..9748)
                     /locus_tag="ACG8OA_000413"
     CDS             complement(9239..9748)
                     /locus_tag="ACG8OA_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975257.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9923..12058)
                     /locus_tag="ACG8OA_000414"
     CDS             complement(9923..12058)
                     /locus_tag="ACG8OA_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFS4975258.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12141..13796)
                     /locus_tag="ACG8OA_000415"
     CDS             complement(12141..13796)
                     /locus_tag="ACG8OA_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4975259.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13798..14112)
                     /locus_tag="ACG8OA_000416"
     CDS             complement(13798..14112)
                     /locus_tag="ACG8OA_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS4975260.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14282..14542
                     /locus_tag="ACG8OA_000417"
     CDS             14282..14542
                     /locus_tag="ACG8OA_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFS4975261.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14556..14870
                     /locus_tag="ACG8OA_000418"
     CDS             14556..14870
                     /locus_tag="ACG8OA_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFS4975262.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15600..16154)
                     /locus_tag="ACG8OA_000419"
     CDS             complement(15600..16154)
                     /locus_tag="ACG8OA_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFS4975263.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16280..18208)
                     /locus_tag="ACG8OA_000420"
     CDS             complement(16280..18208)
                     /locus_tag="ACG8OA_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFS4975264.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(18514..19230)
                     /locus_tag="ACG8OA_000421"
     CDS             complement(18514..19230)
                     /locus_tag="ACG8OA_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS4975265.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19231..21054)
                     /gene="glpO"
                     /locus_tag="ACG8OA_000422"
     CDS             complement(19231..21054)
                     /gene="glpO"
                     /locus_tag="ACG8OA_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFS4975266.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21068..22564)
                     /gene="glpK"
                     /locus_tag="ACG8OA_000423"
     CDS             complement(21068..22564)
                     /gene="glpK"
                     /locus_tag="ACG8OA_000423"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFS4975267.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22700..24160)
                     /locus_tag="ACG8OA_000424"
     CDS             complement(22700..24160)
                     /locus_tag="ACG8OA_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4975268.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24150..25445)
                     /locus_tag="ACG8OA_000425"
     CDS             complement(24150..25445)
                     /locus_tag="ACG8OA_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4975269.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25777..26454)
                     /locus_tag="ACG8OA_000426"
     CDS             complement(25777..26454)
                     /locus_tag="ACG8OA_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFS4975270.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26484..27575)
                     /locus_tag="ACG8OA_000427"
     CDS             complement(26484..27575)
                     /locus_tag="ACG8OA_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFS4975271.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27676..28110)
                     /locus_tag="ACG8OA_000428"
     CDS             complement(27676..28110)
                     /locus_tag="ACG8OA_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS4975272.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28694..30151)
                     /locus_tag="ACG8OA_000429"
     CDS             complement(28694..30151)
                     /locus_tag="ACG8OA_000429"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4975273.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30148..32043)
                     /locus_tag="ACG8OA_000430"
     CDS             complement(30148..32043)
                     /locus_tag="ACG8OA_000430"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4975274.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32101..33876)
                     /locus_tag="ACG8OA_000431"
     CDS             complement(32101..33876)
                     /locus_tag="ACG8OA_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS4975275.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33926..35422)
                     /locus_tag="ACG8OA_000432"
     CDS             complement(33926..35422)
                     /locus_tag="ACG8OA_000432"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4975276.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35616..37238)
                     /locus_tag="ACG8OA_000433"
     CDS             complement(35616..37238)
                     /locus_tag="ACG8OA_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS4975277.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37687..39000)
                     /locus_tag="ACG8OA_000434"
     CDS             complement(37687..39000)
                     /locus_tag="ACG8OA_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS4975278.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39245..39469)
                     /locus_tag="ACG8OA_000435"
     CDS             complement(39245..39469)
                     /locus_tag="ACG8OA_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975279.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39489..39911)
                     /locus_tag="ACG8OA_000436"
     CDS             complement(39489..39911)
                     /locus_tag="ACG8OA_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4975280.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(40055..40765)
                     /locus_tag="ACG8OA_000437"
     CDS             complement(40055..40765)
                     /locus_tag="ACG8OA_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFS4975281.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40783..41280)
                     /locus_tag="ACG8OA_000438"
     CDS             complement(40783..41280)
                     /locus_tag="ACG8OA_000438"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4975282.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41317..41769)
                     /locus_tag="ACG8OA_000439"
     CDS             complement(41317..41769)
                     /locus_tag="ACG8OA_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4975283.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41904..42509)
                     /locus_tag="ACG8OA_000440"
     CDS             complement(41904..42509)
                     /locus_tag="ACG8OA_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS4975284.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42959..43579)
                     /locus_tag="ACG8OA_000441"
     CDS             complement(42959..43579)
                     /locus_tag="ACG8OA_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4975285.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43581..44450)
                     /locus_tag="ACG8OA_000442"
     CDS             complement(43581..44450)
                     /locus_tag="ACG8OA_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS4975286.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44473..44880)
                     /locus_tag="ACG8OA_000443"
     CDS             complement(44473..44880)
                     /locus_tag="ACG8OA_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS4975287.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44899..46188)
                     /locus_tag="ACG8OA_000444"
     CDS             complement(44899..46188)
                     /locus_tag="ACG8OA_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4975288.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(46215..46517)
                     /locus_tag="ACG8OA_000445"
     CDS             complement(46215..46517)
                     /locus_tag="ACG8OA_000445"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4975289.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(46542..48635)
                     /locus_tag="ACG8OA_000446"
     CDS             complement(46542..48635)
                     /locus_tag="ACG8OA_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4975290.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(49124..49888)
                     /locus_tag="ACG8OA_000447"
     CDS             complement(49124..49888)
                     /locus_tag="ACG8OA_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFS4975291.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(49999..50868)
                     /gene="mdcB"
                     /locus_tag="ACG8OA_000448"
     CDS             complement(49999..50868)
                     /gene="mdcB"
                     /locus_tag="ACG8OA_000448"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFS4975292.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(50893..51969)
                     /locus_tag="ACG8OA_000449"
     CDS             complement(50893..51969)
                     /locus_tag="ACG8OA_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS4975293.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(51993..52586)
                     /locus_tag="ACG8OA_000450"
     CDS             complement(51993..52586)
                     /locus_tag="ACG8OA_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFS4975294.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(52590..54266)
                     /gene="mdcD"
                     /locus_tag="ACG8OA_000451"
     CDS             complement(52590..54266)
                     /gene="mdcD"
                     /locus_tag="ACG8OA_000451"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFS4975295.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(54256..54555)
                     /locus_tag="ACG8OA_000452"
     CDS             complement(54256..54555)
                     /locus_tag="ACG8OA_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFS4975296.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(54657..56303)
                     /gene="mdcA"
                     /locus_tag="ACG8OA_000453"
     CDS             complement(54657..56303)
                     /gene="mdcA"
                     /locus_tag="ACG8OA_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFS4975297.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(56310..57221)
                     /locus_tag="ACG8OA_000454"
     CDS             complement(56310..57221)
                     /locus_tag="ACG8OA_000454"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS4975298.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            57341..58198
                     /locus_tag="ACG8OA_000455"
     CDS             57341..58198
                     /locus_tag="ACG8OA_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4975299.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(58385..58747)
                     /locus_tag="ACG8OA_000456"
     CDS             complement(58385..58747)
                     /locus_tag="ACG8OA_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFS4975300.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(58936..59118)
                     /locus_tag="ACG8OA_000457"
     CDS             complement(58936..59118)
                     /locus_tag="ACG8OA_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975301.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            59093..60532
                     /locus_tag="ACG8OA_000458"
     CDS             59093..60532
                     /locus_tag="ACG8OA_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4975302.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(60539..63091)
                     /locus_tag="ACG8OA_000459"
     CDS             complement(60539..63091)
                     /locus_tag="ACG8OA_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFS4975303.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(63205..64536)
                     /locus_tag="ACG8OA_000460"
     CDS             complement(63205..64536)
                     /locus_tag="ACG8OA_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4975304.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(64502..65980)
                     /locus_tag="ACG8OA_000461"
     CDS             complement(64502..65980)
                     /locus_tag="ACG8OA_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4975305.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(66285..68120)
                     /gene="tdc"
                     /locus_tag="ACG8OA_000462"
     CDS             complement(66285..68120)
                     /gene="tdc"
                     /locus_tag="ACG8OA_000462"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS4975306.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        19449 a        14588 c        11704 g        22643 t
ORIGIN      
        1 tgtttagtat atatttttag aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc
       61 acacatttga aaaagagctt tggtcgtttc gaagctttaa aagatgtctc tttcaccgta
      121 cattcaggtg aagtcgttgg atttatcgga ccaaatggtt ctggaaaatc cacgacgatt
      181 cggacacttt tagggattat aaaaaaagac ggcggaacgg caacgatttt tgggcaagat
      241 gtatgggaag atagtttatc tatccatgaa cgtctttctt atgttccagg cgatatctct
      301 ttgtggggaa atttgactgg aggagcgatc atcgatctct tcatgaagct tcatggaaaa
      361 gggagtgctc aaaaaaaaga tgaactgatt caacgtttct ctcttgatcc taagaaaaaa
      421 gcaaaaaact attcaaaagg aaatcgccaa aaagtgggct tgattgctgc tttggctgtt
      481 gattcggatc tctatttatt tgatgagcct acttctggtt tagatccttt gatggaaacg
      541 attttccaag aagaagttga aaaattgaaa gaacagggca aatccatcct actttcttct
      601 catattctca gtgaagtcga gcgactagcg gataaagtcg tgatcatacg cgaaggaaca
      661 gtcgtagaag aaggaacact cgatgaattg cgtcatttga ctcgttcgac catcactata
      721 gaaacagaag cagagaatag tcacttagca caattgaacg gtgtccatga ctttgtgcaa
      781 aaaggtcacc tcgctcaatt tgctgctgat aacgaagctt tcgatgtgat tttagctgaa
      841 gcgtccaaat taggtgtgaa aaaatttgaa tctattccac cgactcttga ggatttattc
      901 atgagacatt atgaaggatg aggaggaagc gaaatgactg ataaattttc acgctggtcc
      961 accctttttt ctcaaaacct tagccgtgac tggaaaaaga tcctgatatg gctagtcggt
     1021 gtcactgctt tttctggtgg ttttgtccct gcgtttgaag aattgacttc cggacaagga
     1081 ttagctggat tatacgaaac cttgcaaaac ccggcaatga ttgcaatggt cggaccaact
     1141 ccagcttcga atgctgctga gtatacttta ggagcgatgt atgcaaatga aatgttgctt
     1201 ttttgcagtt tgtttgctgc tgtgatctct gttttacacg tggtgagcca aacgagaaaa
     1261 aaagaagaac ttggtctgat tgaacttatt cgttcctttc gagtcggacg tcaagcaaac
     1321 tctttagctg tcatcgctga aagtattctg atcaatttgc ttttagcaat atcgatcagc
     1381 tgtacgatga tttttttcca agcagattcg atcactgcag aaggtgcttg gctttttggg
     1441 ctatctgtag gtagtatggg gattttgggg agtgccgttg cactgtttgc tgcacaactg
     1501 atgccaagtt cagccagttc gaatggtctg tctttagcgt ttttaggtgg actttacctt
     1561 ctacgagcaa taacagatgt agctgattcc tccctttcga tgtggaatcc tttaggctgg
     1621 tcttatttga cctatccatt tacaactaac aactggattc ctttgcttta tttgtttttg
     1681 ttcagtctaa tcttgataat cagttcattt gttttagagg aacatcgaga tttgaacacc
     1741 ggctatatcc ccgaacgtga aggaaaaccg tatgctaaac gttctctttt atccttgcgt
     1801 ggtttattat tctatctaaa taaagggatg acaatcagtt ggcttatcac ttttggtttg
     1861 atgggggcgg catacggctc tatctacggg aatatgcaac agtttttaga aagtaacgac
     1921 ttaatgaaac agatgtttat ccaatctggc gtctcaattg aaaaaaactt tacaagcacg
     1981 atcatggtcg tcttgatttg tctagctgct attttaccaa ttgctagcat caatcgttta
     2041 tttacagaag aaacccacgg acgtatgaat cagctgttcg caacaaaaat ttctaggaac
     2101 caactttatt ggaccaatat gttactagct attctatttg ctatattggc aatcctgatt
     2161 agttctggtg cactagggct ttctgctttg gcttcgatga aggatgcgaa agacatggat
     2221 ctattgatgt ttcttaaagc cgggttcaat ttcctgcctg ccgttttagt gtttatcgga
     2281 attagtggct ttctattcgg atggcttcct cgaatgggta aacttgctta tatctactta
     2341 gcttactcct ttgcactcaa ttactttggc ggaattttgg atttaccaga atggttttcc
     2401 aaaacagctg tccctagctg ggttccacgg atgccaaacg attcttttga tctttccgtg
     2461 ttccttacgt tatcatgtat cagccttgca ctgttcgtcc ttggttctat tggttatagg
     2521 cgaagagact tactagaagg ataataaaaa agcatgtaca atttcatgct agcctccaaa
     2581 tctgggtcaa aaatccaaca tttggaggtc atttttttat ttacaagtct tctaactgct
     2641 ctttatcaat gatccagact tctcgattct ttctttttac ataaccattc tcctctaata
     2701 atttaaattt tctagataaa gtttcggggg tcgtaccgat aaatgcagca agttctttca
     2761 ttttcattgg caggaaaaag tgatccttcc ctgtcgcttt agctaagtca agcagataga
     2821 cagcgactcg ttcttctact ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg
     2881 ttaatttctc aacgttgatt gctaaaagtt tcagactaag ggcgggataa tgcagtaaga
     2941 gttccgagaa atctttttgt ctcaatatgc aaatttccgt tttttctagt gcttcgccaa
     3001 ataaagactc atttggaacg cctagcaact ggctctctcc ctcatatccg cccggctcta
     3061 ttactcgtaa aagctgttcc tttccgctag cagataattg atacactttc atactgccgg
     3121 aggccacgat cactaattga ggttctccat tcggcgcaag aatctgttct cctttttcca
     3181 catagtgatg tttgacaagt gcgtgtactt tttcttgatc ttgctgtact aagtgattga
     3241 acaaaggaac aaatgaaaca cataaatgat catgactcat aatctctcct ccttctaaac
     3301 agctatttct aataaagaaa cataacttcc cttattccaa ctgataattt tcttttatca
     3361 tagcataaac gtcaagcgaa ccagatacca gcaaatagag tggatttctt tttgtccctt
     3421 gctttatttt ccttcttttg agaccttaag taattgattc ctatcaattt tttaaatatt
     3481 tactttttga ataatcaaaa acaaaccttc actcgcaagc cgagagtaag gtttgttttt
     3541 cgcattgctt atttcgtgtt gtctattgaa atttttccgc acctagcttt tgatatagtg
     3601 gtgctagttc ttgtaaggtt tcagcggtta tttgctcaac gtgttcgatt tcttgcggat
     3661 agaagaatag tttacccatt tgaaaggttc ggtctctcat cgcttttttc aactcttctt
     3721 gtttctttgt atcggacaaa tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg
     3781 gttgcggcca tatgtaataa tccttgatat cgactgtttt gctccactct ttcagtatcg
     3841 caagcaactg gttatacgct gcaatgctac catatcgttc ttcgctttta taatgttggt
     3901 acatcaaata gatttggtat tgtttttgat tcaacaaaac tgctagacag gcattctcgt
     3961 cttgacgaga ttcgcttcgg tacgcgctcc aaaaatgact gcgtaaattc cagccattcg
     4021 tccagctttc gattttaggt ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct
     4081 ggagctgaat ctctttccat ttttgccagt gttctttata ttctttcttg atttgttcaa
     4141 tcttatctgt tggatggctc tctttgagct gtttgaatgc gtagattggt cgatcaaaca
     4201 actgataatc tgattttcca agttccattg tttcgcctct ttccgttatc ttttgtattc
     4261 tacctgaatt ttgcgatatg gacaaataga ggttgtgaaa aagctgttct gcatcaagtc
     4321 ataagaacag ccaaacaaaa aactgcgatg agcatcaccg cagttttatt gaacttaagg
     4381 ttgattgatc acatctaaaa gcgttgaaaa taaaatggca ctagaagcag ctcctgggtc
     4441 gatatgtccg attgcacgct ctcctagata ggaagctcgt ccttttttag ctaccaagac
     4501 tttggtgtgt tcttttgcct ggtcaatgat ttcttttgtt aaattccctg ccttcaatgc
     4561 ctctgtgact ggcagccaaa catctaacat cgttttatcg tcagcttctg cctttcctcg
     4621 gctttgaata cccgctgttc cctgctcgat cacttcgcct aatttttctt gagaatcaat
     4681 agaatcgacc tcttttgtcg ctttcatcat gttcatgaat gcagatccgt ataacggacc
     4741 agaagcccca ccgactttcg atatcatcgc catagagaat actttgaatg tatcgctgat
     4801 tgtttcagga tgatcggctt gaaaagcttc ttcataagct gaaacccctc ttcccatgtt
     4861 atttccatgg tctccatcgc caatcggggt atctagatca ctgagatatt gtttgttctg
     4921 attgattgcc tctgcaaatt gcgtgagcca ttcttggatt tcttgaacgg ttagtttcat
     4981 attatcaccc ttttatcttg agatttttta ccatgaaatc gtctgtacat tgctttctaa
     5041 agaatctttc cattgtgtct ccgtcaagtc gatcagtgtt agagaaagac cttgcatatc
     5101 tagtgaggtc ataaaattac cgacttttcg gaaaactaca ttgacgcctc tttcttccaa
     5161 cagagctaaa acatcattca tgaacacgaa ctgttccatc aaaggcgtcc cgcccattcc
     5221 gttgactagt acacctgctt cttttggtaa attcgagtaa tcatctaaaa tcttagtaac
     5281 aagttccttt gccagtactt tagaaggctg catcttttct cgacgatagc ctggctcacc
     5341 gtggatacca acaccatact cgatctcatc ttctcctagt tcgaatcctg gtttccctac
     5401 ttctggtaca gttgctgctc gaagcgctag accgatcgtc ttagttgcct gtacgacctt
     5461 ttctcccagt tctttcaact cagctaggga agcaccgttt cttgctgcat cgcccacgat
     5521 tttgtgtaca aggacagtac ctgcaactcc gcgttttcca gcagtatacg tactattttc
     5581 aacggcgata tcatcatcta cgatcacact ttctacttca atatcgtcca tagcagctaa
     5641 ttcctttgcc atatcgaagt tcaatgcatc ccctgtataa ttcttaacga tcagcaaaac
     5701 tccttttcca ctatccgctt ccttaatagc cgtctgaatc tgatctggag ttggagaagt
     5761 aaacacatca ccaagcaccg ctgcacttaa catcccttcc cctacaaacc cggcatggga
     5821 tggttcatgt ccgcttcctc caccagaaac caaaccaact tgttccgctt tctgattctt
     5881 ggctacaaca cgggaagatt ctacacgatg aacaagttct ggatagcttt ttactaatcc
     5941 ttccagcatt tcttcgacaa tattgcttgg atcattcatg atttttttca atttattccc
     6001 atcctttctt tccggcattt tgtgcttctg ttaagatttg ctgagtatca tctgttatac
     6061 cggcagtgat ggctgctgca aaagctcctt ccactaaagg cgcatccacc agatgataac
     6121 gttgctgctg ctcttcttcc aacatgtctt ttgccagttc cgagttcaat accgcgctac
     6181 cgatatccgc aaaaacaaaa taactagctg catcgcttga ttcattgact gcttctacga
     6241 ttttcatcgg gtctgatccg atctcgccat ttattccgcc gagagaaaaa atttgcacct
     6301 cttcggattt ttgcatctgc tcaatcattt ctttaatacc atctgttact ttttgactat
     6361 gagaaataag caaaatactt tttttcacaa gaatcttcct ttcttttaca tagttgctaa
     6421 cgattggttt cgctttcatt aatgaacaat tcaagtatat aataaatttc taattttggg
     6481 aagatatgtg tagtgcttct tgaagtttgt tatactttaa ttaaaagaga ggagtgatcg
     6541 aatgattaca acaacaacga acagtgtaga gggaaaagcc gtagtagctt ataaagggat
     6601 cgtttttggt gaggtcatta ctgggatcca tgcatttaaa gacttggagg cggggttgcg
     6661 aaatatcttt ggcggccgct caaaaagcta tgaaaacgaa ctaatgggtg caagagaaga
     6721 agcacttgcc gaaatggccg aacgtgcaaa agcattagga gctgatgcga tcattggcgt
     6781 aaaaatggac tatgaagtat taggcagcga caatggcatg ctgatggtca catgtagtgg
     6841 aacagctgtc tccacgagat aagtagggga catctgtttt aataactaaa gagagatggg
     6901 acatgtagtg gcactcaaga attagatttt tcagtctaac ttcgaggtac tctaccttcc
     6961 catcctctct tttttatttc tttttgcgga tgattccgta agctgctgcg actaacacac
     7021 ccgcccctgc taaggaataa ctgacagaag ctgttttttc atttgttttt ggcaaagtct
     7081 gacttgttga tttggatggc tctgtcgttt gctgaactgt ttctgtcgtg cttccagagg
     7141 tcactgattt ttctcctgcc gaatgcaaaa tatcttttat tcgttggaaa ctagctgcta
     7201 agtcagcttt ttttgcttca aaatcattag caactgtttg tgcttcgctg atctttccgt
     7261 ggattgcttg actggccgct gtgatatccg cttgagtttg acttagcttt ccagaagctg
     7321 tttgagcaac ctgatttaat tgctgattga attcttttgc ttgttggatc ttcccgtcaa
     7381 gatctgtttg gacagcttcc gcttttgctt tcgcttcggc aagctttttc tcaagttgtg
     7441 cacggtcatt tcccacttta tttttaagat cagctaaatc ttgatcgatt gctgcttggt
     7501 cttctttcaa tttgtttttt gcagcttcta atgcttcttc gagttctttt cttttatttt
     7561 ccaagtcttg caaatgatcg atttggtcag aagaggtaga actatttatt gatgtatctt
     7621 ctgaagatga aatcgtttct cctgaattat ctgtcggtac attggaagtc atttctgttt
     7681 ccgaggcacc gttttcagca ccagaagagc ttatagttgt tgaaggttct gtttgagcag
     7741 gttcagaagt ttccgcaaaa gctgtctgtc ccaataagaa aatactggaa acagcaattg
     7801 gccagattaa cggcttcatt ctttttttca tcacttttcc catccttact actttttaac
     7861 taatttctta ttataagcaa aaatgtttgg aagaaccata aacacaacag gctgtttgtc
     7921 taaaaaacgg tgcatctttt atttttttgt acgttccggg cttttccatg tcccgcttcc
     7981 ataacctaaa atcgttttga ctgctggaat gaaagtcgtc acgatcgtta tcgcgttcac
     8041 tatccagaac aaaagcatat atagtggtgc gaaaagaagg tatttcaatt ttcttcgtcg
     8101 atcatctaca atcagcgaag ctagcaactg gaaaaatcct gcgaccattt caaagcagat
     8161 gaacacaaaa ctgaatgtaa aagtaatata aatctgttcg tagtttcctt gatatacata
     8221 atgcccgatt aatccagcaa aaagaactac ggaaatacaa aagaatattg accagacaat
     8281 actcaatgtt tgatcaatga agatgatcgt tcttccaata tgtttaaaag gatggagcat
     8341 cacttttttg aaattcgtca gccagacttc tgttccacct tttgcccaac gttttctttg
     8401 tcgatatagc atcttcaacg tcacaggtac ttccatgaaa aagataatct caggagcaaa
     8461 aacagataac cagtcattta actgatgatc ccaagcaata ctgatgtctt ctgtcgctcg
     8521 atcttgtctg aatccgccca catcgatcaa ggcttctttt cgatacatcg tatttgctcc
     8581 actgtacgca taaagaccac cgaaaacagc tgactgtgtt cgtttaatga tgcccacgat
     8641 acttgagaat tcaaccgttt gagattttgc aatcaatttg gtacgatttt ggacatccat
     8701 atttgcagtg accgcagcaa tatgtcttgc accagggcga atgaaataat tcacatacct
     8761 aatcagtgca tctggctccg gtaccgtgtc tgcatcattg cttaaaatca atttcccttt
     8821 tgcaaaggca agtccgatat taaacgcgtg ggcttttcct ttattttttt cgatccgaac
     8881 cacacgaaga ttagcgtatt ttttcatcag tctagctaat atttctggtg tttggtccgt
     8941 tgaaccatcg tctgtcacta atacctcata gttgtgataa ttcaatttcg tcatcaaata
     9001 ctcgattgta tcttcgatca caatttcttc attatgggct ggcaccatga tcgtgatgaa
     9061 cggttcgaca ctcaagggaa catctaccca ttctttttgc ttatgtttgt ataaaaacac
     9121 ataacaccag acaccaataa accatacaaa tccgcctaaa atcggataag aaacgagaaa
     9181 aaatacataa tagacaaaca agtatctaag aataaattac ctgtatgggt gattgagatc
     9241 atttcagttc caccccattt tctttataca aatctgcaat gaatgtcgta tctagatttt
     9301 tctcttcgga gacagaataa aaacggaccg tttctctctc ttcttttgtt ccaaaacgtt
     9361 ccgtatacac ttcttcaagt aattcttgtc tttttttcaa tttttcatct tcatattcaa
     9421 aagatttatt tacttttttg tatagatttc gattgttcca cctagtcaat acaaatgagg
     9481 taatcaggaa actgaaaaaa atcaatacaa aaaagccgat taagaaatac agcattttct
     9541 tttctgataa aaaagcgtac aaggaaaaat ccttggctct ttctggtctc gttaatggga
     9601 aaaagaccca tataaaagga agaacaacga tcatccatcc taaaagagcc actaacgttt
     9661 gccaaatctt taaccagtaa ttaccttttg aaaaatactt atctttcacc aaaattgatg
     9721 caacttcttc tcttgttact tttttcatat cctcacctca cgtttttttg ttacgacgta
     9781 ggagtttacc atacatttca tttcttttca cataaagtgt tcctaaattt ttcctaagaa
     9841 aaagaacaaa acatttctca tcagcaaatg aaaaatattt tgttcttttt atcagttgtt
     9901 ctttttattt tttatctctt aattatgttg ctctttccaa tacgacaatg cttttcattc
     9961 ctctagatat cgccgtgtac atttgtctgc gtcttctttg ctcatctcca tcgttttcaa
    10021 atggatataa gattacatga tcaaattcca gtccttttgc tagactcaaa ggaatgattt
    10081 ggcagttttc taatccatat gaattttttt taatcgattc ttgtagcgtt tgggtttcct
    10141 cttctgtttg ggtgataact gccgcttttt ctccttttag agaaaccaaa atctcagcta
    10201 gtattttcaa caattcttca gaggatcgaa aagagcggta ttcgggttct tttccttgcg
    10261 gtcggatcga tacgatgtcg atcttctctt gatccacagc ataggttttg aaaagctctg
    10321 tgattgctcc gctagaacgg taactatttc taagatcata ccgatggatc ggcagatggt
    10381 agtcgtcgaa acaacgcata atatcagcaa aagtagtgga agaattaaag attgcctgat
    10441 tctcgtcacc tactaaagta aagtgagttt ttggaaataa ttcgatcaat agacctaatt
    10501 gtgccagggt ataatcttgt acttcatcaa tcaatagata gcgaaatgct ggtacattta
    10561 ccttttcaac aagtaaatga cgaatagcta atataatgac tgcttcatca acagtacgtg
    10621 tgggttgcca agcataagct gagccatatc gtttttcata aattctttca aacaactgat
    10681 gctcatccac ccatgccatt tcttcgattt gtctggttat ttttcgatat tttttttgta
    10741 aaagtttcct tgcataagct acaatacttt gttccgaatc atcagaaatc aatttaccga
    10801 ataattccaa ctgcctatct tctgtcaacg agagtacctg attctgcaca gcaggacttt
    10861 ttgcttgttt caaaagatga ttcttccatt cgctcatcag tgcctttttc gttgcttgaa
    10921 gcctatcgat caatttagga tgagcaggaa ctttctcata tattttttcg atcgtttttt
    10981 tagaaaggac tgtttttcca tttttagtaa gtccacggat aaaattcgga tgatctaaaa
    11041 acaaaggatc tgattgtttt agattatcga taaacttttt gctacgcaaa cgttctgtct
    11101 gctccgatac attttctccc gtgatacgtt tgaaataagc ctcttcacct tctatttcct
    11161 ctgctgaaag ctgtgaaacc agctgtatga tggttagatt cagtggattt ctttcaccta
    11221 atgatggtag gacattcgaa atatagtcga taaaccgatg attaggtgat agaatcagca
    11281 agtcatcgga tgtcatcgta tctcgatata aatagagcag atacgcaata cgctgcatga
    11341 tagcagaggt tttaccactt cctgctacgc catttaccaa gatattttga cttttcacat
    11401 cccgaatgat tgtgttttgt tccgattgga tcgtagcagt gatagccttc atttcattgg
    11461 tctcatcttg gcctaagact tctaataata cgtcatcttg aatagctaca gacgtatcaa
    11521 aaaacttgac caaacgatct ctttccaaaa gaaattgtcg tctttcttta ataaccgtat
    11581 cgattttatt ttgacgaacc gtataagaag atggaccaat catgttattg taaaaaagtt
    11641 cggcaattgg tgaacgccaa tcataaacaa gattttcatc tgcatcatct gtgaaatttg
    11701 ctgtaccgat ataaaaggat tcttttggct cattatccag aaagtctaca acgacttttc
    11761 caaagtaggg acttttcaat aaacgttcga tctttgctag ctgattttct gcgtttttct
    11821 gtttgatatt catctgatcg atttctctat ttttcatttc aatcatcgaa aaagtatcca
    11881 ggttatctaa gtaactgtca aaattcaatc gaagctcgcc agccatttct ttcaacgact
    11941 ggtttccttc tatttttgcc gtatccatcg attcccgcag ttcgtttttt ttatcaatta
    12001 gcttttgata cacgttcttc agatatgctt cttctaactg tttttgtgtg ttgaccatac
    12061 acattctcct ctttttttaa agtggtggaa ccgattagtt tacctctttt aagagtcgaa
    12121 tgcaagcttt ttaacgagta tcatttgtta ggatatatca gttcttcagg tcggacagtt
    12181 tgatataaag aaaatgcacc atcaaggttt tttacgtggt agccagactg cttcaagatt
    12241 ctttccgcta gatagctacg taatccgcta tgacagctga cgatatattc ttgttggctg
    12301 tctaattcat ttaatcggtc ccgaagttca ttcaaaggga tatgtttggc ttccttgaat
    12361 ttgccctttt ccaattcttc tgctgttcgc acatctaata aaatttttcc ttttgctagt
    12421 tctgtcggca attcatgcca ttgtacggtt tcactaagcc cttctgcaat gttcattgct
    12481 gcatatccta gcatgtttac tggatctttg gctgagccaa atggcggtgc atacgtcaat
    12541 tctaattccg gtaaatcgaa gatcgtcaaa tgccctttga ttgctgttgc taaaatatcg
    12601 atccgtttat ccacaccttt tgccccaaca ccttgtgcac cataaatctc gccagtctct
    12661 ggatggaaga tcaacttcag taaaatatct gttgcttctg gataatagct tgcatgatct
    12721 tttcctgata catgaatgac ttcaaaagac agttcgtttt ctttggctgt tctttcactt
    12781 aaaccggtgg atgcggcagt cattccaaac gttcgtataa ttgctgttcc aataccgcct
    12841 tgatttcttc gagaaattcc ggaaatcgtg tccgcaactt gtctaccttg acgattggct
    12901 ggtgaagcaa gagaaataag tgcatctttt ccagtgattt gctgttttac aacgatagca
    12961 tcccccaccg cataaatatc aggaatattc gtttgataac gttcgtctac taggataccc
    13021 ccgcgtatgc ctaattcgat tccagcggtc actgctagct ggttatcagg acgaacacca
    13081 atggataaaa taactaaatc agcagggatg gtacgcccat catccagtcg caatcgtttc
    13141 ccgtggtctt caaatccagc gacagcatga gaagtaatca cttctactcc ttgatgagac
    13201 aattcttttt ctataaaagc tgccatttct tcatccagtg ttggaagaat atgtggtgct
    13261 tgttcgatca ccatgacttc taaacctctt ctttttaggt tttctgccat ttccaatccg
    13321 atgaatcctg caccaacgat cacggcacgc tttggctgtt tttctaatgc atgcatcact
    13381 ttatcgatat ctggtacatt tctaatagaa aaaacattgt cggcttctgt tatccctgga
    13441 atcgatggaa caactggttt tgcaccagga gataaaacca atgcatcgta ctgttcaatg
    13501 tgtttttgac cctcatgtac gacttctacc gttttgtttt cgggaaagat ggctgtcact
    13561 tcatgatttg gacgcacatc taaacaaaaa cgagcggata atgattctgg cgtttggaca
    13621 agaagatttt cccgttcact gatttctccc gaaagataat aaggcaagcc gcaatttgca
    13681 aaagaaacat acggtccttt ttcaaaaaca acgatttctg catcttccat taatcggcga
    13741 agccgtgtag cagcagacat tccacctgct acacctccga caataacgat tttcatctta
    13801 tttccctcct actgttcgat caaaccattg attcatgcca ccacgtacat tcactacatc
    13861 ataaccagat ttctttaatt ctttagctgc ttgtttactc ctcatccctg attgacaaat
    13921 cacatagacg gtcttttctt tgtctccatc atatctgttt atactttgta atggaacatt
    13981 tttagctcct tttatatgcc cgccccggta ttctgcaggt gttctaacat caagtaacgc
    14041 tggattttcc aaaagctttt cctttaattc tgggactgtt atcgatttta ttgattgaaa
    14101 tagcaaattc attttctgca cctcatttac atttttataa atacctcatg gggtatatta
    14161 aacaggagct ttattaatat gtcaaccaga atgactcttc atttttttca ctacttttta
    14221 tatttgcata aaataccgat aaggggtatt atctaattat aaaaatagag gagaatgtgt
    14281 tatggagatt gataataaaa atatcctgaa ccgtttgaaa cgtacagagg gacaaattcg
    14341 tggtatccaa aaaatgatcg aagacgaaaa agaatgtatg gatgtcatca ctcaattaag
    14401 cgctgttcgt tctagtattg atcgcgtaat gggcatgatc gttgcagata atctgaaaaa
    14461 ttgctttgag aaacctgata gtaatccaga agaacaagcg gctaaactag aacaagcgat
    14521 caagatgatt attaaaaagt aaaggaatga tttcaatgaa agtcgttttc catattgatg
    14581 aattagaaaa atggtcagaa acagggaaaa acgtaaaaaa cctgataaaa gcttctccag
    14641 aaacgacgat tgtcgtatct gtaaatggca tcgcgattac cggttatcta gactctgcca
    14701 atgcagaatt cttagatttg caaggagtcg tttttcacgc ttgcgccaat gctatgcgtg
    14761 ctaatcatat ttcagaaagt tctttacctg aacaagtaat cgttgtacca gcaggtgtcc
    14821 ttgatttagt tgaactgcaa tcccaaggtt atgcttatat caagccttag tcagtagagg
    14881 ttgcgacaga agtgatcagc ttcaagaaat ttcggcttat ttttcgaaga agctacttct
    14941 ggaaaaccgt gaatcaaagg ttgtgaaaac gctgtcctgc atcaagtaat aagaacagcc
    15001 aaacaaaaat agcttctcat atttttcgtt tggttgggct tattaccgca gatgcaagct
    15061 tttgaacacc gtttaaggaa gaggttgtga aatcagcggt ttgacctaag tattagagaa
    15121 aaaaacggga aaatagctcc tcatattttg ccgttttttg agctaatacc gaaggtcagc
    15181 tgattgaaca ccgtttaagg aagagattgt gaaaaagtcg cttagtttcg agtattaaga
    15241 aaaattttgt gaaaatggat ttctacattt ttgcataaat ttggcttaat accgagaaac
    15301 tggcttttga ataccgttta aggaagaggt tgtaaaatca gcggtttgac ctgagtatta
    15361 gagaaacaaa cggaaaaata gctgctcata ttttgccgtt ttttgagcta ataccgaagg
    15421 tcagctgatt gaacaccgtt taccaagagg ttgtgagcct accatttaac tccgaatcac
    15481 aagaaataat ttcaaaaaca acttctcctg tttttggttt ggttggctta ttatcgagaa
    15541 gtagctactg ggagtctatt tatcaaaaaa aggaatgcga cataggtcac accccttttt
    15601 tattttcttt gaaacatctt tcggtactcg acagcataca agacaaaaga ttgtaaaaga
    15661 ccgtaagtga tgacggcaaa aatgactctc acccacagcg ttggtaattc tgggaaaagc
    15721 agcaaaagga tcatgccaac atagatgaca gcagtcgcta ctttgccata ccacttcgct
    15781 ccacttaatt tgatattttt agatagcccg attaatccag ctacaaacat aaagccttct
    15841 tttatcaaga atagaccaaa caacagccaa agccacggtc gataccatgc catagacaaa
    15901 atcagaaaaa gttgtgtcaa tttatctgca aacggatcaa gcaccttccc taagtctgtt
    15961 acctgattat atctccgagc aatgaccccg tctagataat cagacaaacc tgagacaaca
    16021 atgaccgctc ctgctatata aaatgactgt ctcagcacaa aataaagata aatcggcagc
    16081 aaaaaaatac ggaatatcga taaaagattc ggaatcgttt gtatttcttt acgccagtct
    16141 gtgcttaatt tcattttatc gccctacctc ctcagcactc ttttcttttt attttagctt
    16201 acacggggaa taaaaccaat gatttcactg tttttatttt tctttacagc cactgatatt
    16261 atgagctaat ttgagataat cacttgctgt tttcatcgtt tactcttctt tcttcctgtc
    16321 gtttgcgcat ttcttttact agtactttat gagctttttt tgatcctcgc tctaggtcac
    16381 gctgccgttt acgtgtttta taatcgataa acagactgtc taaatcgtaa cctgtcgggt
    16441 acagatcttc tgctgaacgc accagacgaa cacgctgacg aggcacgtgg attatttttt
    16501 tgtctaaata tacatcaatc gtatcttcac ctatatcttc ataaactaac gcttcttttt
    16561 ggtattcatt gacaaatatc cgatcacctt ttgcaaagag ctggcgttct tttttattct
    16621 ccttcctatt tgtaactgct gtcttaggaa gaaattcttt tttctgtgtc cgataggctt
    16681 tttccgccaa gtaacgctct gcttgatgga tcaatttcat cgacatatcc attttatgag
    16741 caatccacaa agcttggctt tctcctgttt ctccaacccg caactgatat ttaggacgaa
    16801 gtgcttcccg atcgaaagcc atagcagccg gcacaaaatc ttcatgatct ccggcgaatt
    16861 tcttgatttc tccataatgg gtcgtggcaa tgatcaatgc cccttgctca tacatagatt
    16921 ccatgattgc gatcgctagt gcagcccctt catttggctc tgttccactg ccaatctcat
    16981 ctaaaagtac caacgtattc ctttttatct ttttcaaaat agcagcgatg ttttgcatat
    17041 gaccagaaaa tgtactcaac gcattttcta aattttggtg gtcaccgaca tcgacgaaaa
    17101 tctcatcaaa gacagcaagc tctgtccctt cttttgccgg aacttgcata ccgaacatcg
    17161 ccatcaaagt caataaaccg actgtcttca acacgactgt tttgcctccg gcatttgctc
    17221 cggtgataat caagcctcga tagtctttac cgatttcaac atccaatggt accgcatgct
    17281 caggtaaaaa tggatgtctg ccttgcttga taatgatatg ttccgattta ttgattctag
    17341 gtgtgcttcc attgatttct cggctaaatt ttgcccgagc aaagatgata tctaacaccg
    17401 tgatcgtttc gatcaataaa tcgatcactt cttcgttttc tgcgatagca ccagtcaatg
    17461 ccgccaggac ttgatattct tcactgattt cttcagcttt taacaactga tagtgttcat
    17521 tcaatttaga aatcacagtt ggttcaataa aaaccgtcgt tcttttattt gattcatcga
    17581 taatcgttcc atctatttta tttttatagc tggctttgat aggaatcgta tagtattcgc
    17641 ctttttgaac aatcattgct tcttggatca tttctttgtt tttcggatga cgcaagaatt
    17701 tcagtaattt tgactggatt tctttttcga ttagttgtaa ttgtttgcgt actttacgca
    17761 aattacgtga cgcgtcgtca ctgactttat tattttttat tttctggtca atcaactctt
    17821 ctacatttat caaatcagga agatgtttgc tataagcaaa caacaagggt gtctggtatt
    17881 gattcttatc aaaaaacttt gtgatcatcc gactgcttct aagaaaatcc gcatattcga
    17941 tcaaatcggt tggctgcaga atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc
    18001 tcggcaaccc cataaatgga acatgctgac tgctttctaa gatcaagcgt gcttctcttg
    18061 tttcttcttg acgatccaag acagtttgta ggtttgtcga gaccgtcata tcgctgattc
    18121 gttttttgct atagtttcca attgctcttg cttggatttc ctttttgatc cgatcaaatt
    18181 gtaattgact aatcatttcg ttgtccatat tctctccttt tcttgaagag atacctgtcc
    18241 caaaataaat aagggcctag agataaataa aaggatagaa cacagcttaa aaaagtcatg
    18301 tcctatcctt attagaatac aaagatattg ttttcctgac aggtaccgtt tcataagcac
    18361 accaaataaa ctttgtttat tggctattcg acttactgat tagataccag ctacactcac
    18421 aaaacatacc ccattcttct aggtcttatt tattttccac ttaactatag caagacacat
    18481 tttggttgtc aaactttagt atgttttata gatttacacc attaatgaat agacacctac
    18541 cgcgataatt gctccgatca ttggacctgc gattggtacc catgaatatg cccaatctga
    18601 agttccttta ttagctattg gcaataccgc atgtgctaat cgaggaccaa agtcccgagc
    18661 agggttgatt gcatatcctg ttggccctcc taatgaaaga ccgatggcta agatcaatcc
    18721 acctacagcg aatacgttcg ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc
    18781 aagaactaat acaaatgtac caattaattc tgtgatcaca tttgctggat aattccgtat
    18841 agcaggacct gttgcaaacg ttcctaaaat cgcgctttca tctttcgttg cattccaatg
    18901 tggcaaataa gaaagccaaa caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat
    18961 gatgaaagga acgacaagat tccaactaat tgcccctgtc atcgccatag ctagagagac
    19021 tgcaggattc aaatgagctg ggcccatgaa tccagaaata tagacagcca ttgttacagc
    19081 gagtccccaa ccaaatgcga tgacaatcca gccagaagcc tgtgctttac ttttgtttaa
    19141 attcacagcc gcacacacac catctccaag caaaactagg atcagtgtcc caataaactc
    19201 tcccatgatt tgagtcatat cactgttcat ttatttctcc ccctttagtt cccgcagatc
    19261 tgattctgca atcaatgctt ctaaacgttt agtctgttgc gccttctcct cttctgtcca
    19321 ttcaaaatat ccagcaattg catcgatcac aggttgcttg atctcatcaa gctgatctcg
    19381 ttcaaacaac agatgatttg tacgacgaat gagataatcg cctggggcca aaaccatttc
    19441 ttcttctaaa ccataacgta accgagctga ctcagcgaga ctcaaaccag gataaggtgc
    19501 catttctttt gctaattcaa agatacgtcg ggcattagtc ccgtaaaaat cagcgatata
    19561 agtagcatct tcttcctcta aaccggctgc tactccttct tttgctaatt ctgtcactgt
    19621 ttcttctaat ttagttggat cgaagtttcc acctgaaatc tgatattttt tagagtcgat
    19681 ttctttcgtc tctattccgt attcttcttt taataattgg cgtatcaatc gcaaagctcc
    19741 ttctgccatc ttacggtaat cggtgatttt cccaccagat aaagtcagca aaccatctgg
    19801 ttctctttct aaagaactac ctctggaaat cgtagaaggt gctttttcat cacggctgtt
    19861 ttccaaatgg ttcaacacgt cttctacttc agcacgagaa actttgtttt ccttatattc
    19921 acttactgta tcgacaactt tattgaagct cttgtctgaa atcgatccat tatctccacc
    19981 attgtaatca gaaccagaat taccaatcaa cagtggacga agtcctgccc agcttgcttc
    20041 gatatcattc aatgtgatat ttgcttctgg atagcgatga ttgatcacat ccaatagata
    20101 atctacatct tcttgtgtga ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt
    20161 cgtaccaaag taagtcttgt tttctcttgg aatagcaaaa accatccgcc catcttgctt
    20221 tcctgtatcg aagtatgtgg gttgcggtac aggcagtttt ttcgcatcta caactaaatg
    20281 gatcccttta gttggacgca ttttaggaga gactggacgc gtaaaattca agtttcgtac
    20341 tttatctacc caaggaccac tcgtattgat cactaatttt gctttgattt cgatcacttc
    20401 atctgtcagc aggtcacgag ctttgacgcc aacgatttga tcgccatcat ataaaaagcc
    20461 aaccgctttc attttactta ctagataagc cccatcttcg gcagcctttt tgatattatc
    20521 aatcactaaa cgggcatcat tgttgcggaa atctagatag acacccgcgc cttttagccc
    20581 ttcttttttc aaaaacggtt ctctttccaa tacttcttct ggtgtcagcg tataattctc
    20641 atatttggtt cctgtcacat ttgccagttt gtcataaagg tccattgcta ctttgaccga
    20701 gaacatattg aaggttgttg ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg
    20761 ttttgggatg tgtggggcaa tactttgtac gactgcacgt tcaccaactg tgtcagctac
    20821 tacttccaca tcaaatgtct tcagataacg aataccgcca tgaacaagtt tggtcgagcg
    20881 agaggaagtc ccttctgcaa aatcctgcat ttcgatcaat cctgttttga ttcctgatgc
    20941 tgctgactgg actgccacac cggcaccagt gattccgcca ccgatgatca acagatccaa
    21001 ttcttcttgc tgcatttttt gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat
    21061 ttcctcctta ttcaaatgct tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt
    21121 tttctcggcg ctcttcctcc atttgaactt caaatagctt gccagcttca taaaattcgc
    21181 ggatctcgtc tgtatctttc caataaccga cagctaatcc tgctaaaaat gctgccccta
    21241 gcgccgttgt ttccaagttt tctgctcgtt ggatcggtac attcaagata tctgtttgga
    21301 attgcatcag atattcattg tttgctgcac cgccatctac tttcaataca ggaatcttga
    21361 ttccagtgtc atcttgcatc gtatcgatga tatctctcac ttgataagca atcgactgta
    21421 aagtagcttt aatgatatct tcttttgtcg taccacgtgt caagccaaac atagcccctc
    21481 gtgctgcctg atcccaataa ggagcaccta gcccaacgaa tgcaggaaca acataaacct
    21541 catcttcgct tgtagagcgt ttcgctgcgt cttctgaatc gctagctttt tgcaacattt
    21601 gaagtccatc tctcagccat tgaatcgaag aaccagccac aaaaatactt ccttctaaag
    21661 catagtaaac ctttccattt atgccgtaac caattgtcgt caataaatta tttttggata
    21721 attgcggttt ttcaccggta ttcatgacaa taaatgaacc tgtaccataa gtattcttga
    21781 ccatgcctgg ttcgaatgcc atttgtccga ataatgcggc ttgctggtct cctgccatcc
    21841 ctgaaattgg tgcttcgctt ccataaaaat gataaccttg cgtataacca taaacttctg
    21901 agttagaagt gacttttggt aacatcactc gaggaatatt tagtaaatct aaaatctcct
    21961 gatcccaatc tagctgatgg atattgaaca acatcgtccg actggcattc gagtaatcag
    22021 taacatgtgc ttgtccatcg gtcagtttcc atactaacca agaatcgatc gttccaaaca
    22081 tcaattcgcc tttttcagct cgttcttgcg cgccttctac atgatcaaga atccagcgta
    22141 tcttcgttgc tgagaaatag gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt
    22201 gatagccttc attttttaag ttatctgcaa ttccagctga ctgtctggat tgccaaacaa
    22261 ctgcatgata gattggacgt cctgtatctt tttcccatat tactgttgtt tcacgctggt
    22321 tcgttatgcc aatcccagca atctgatttg gtttgatccc tgattcgatg aacgctccgg
    22381 caataactga ctgtacagaa ttccagattt catttgcatc atgttccacc caactttctt
    22441 gcgggaaata ttgcgtgaac tctttttgag aactgccgat gtgtcttcct ttcttgtcaa
    22501 agataattgc tcgtgagcta gttgtacctt ggtcaatcgc catgatatac gttgattctg
    22561 tcattgtgat tccctccatt atatgcaaac gctttcttta actactctta tcatactact
    22621 gttttaaaaa aagaaactgt caaaatttta ttcttttttt ctttttttga cagtttttta
    22681 ttatgaagct attttttaat caattctttt cttttttcag cgtctctatt cttcttttca
    22741 atcgaataat gtcgtaaggt gacgcaaatt ctgacagata atattcacac tgttttttgt
    22801 ttctctggca aaatgtgatc accaaatcat attctttacc ttcttcataa cttccaatct
    22861 caataccgcc gattcccctc agttctctga tcagatattg ttgaaaagca atacgataaa
    22921 tcggcagact ttctaaatcg attccaatca ctactggttt tgcaatataa aaatcgatca
    22981 tcaaaagaac attggcatac tggatcatca ggtaactaag ctcactatct tcagctcttt
    23041 tcctcggcaa ttgctgcacc gctgtttcct gtaatttcgc cagtaactgt gttaaggaat
    23101 gcccgagcat tttctgctgc tgttccaaaa gccgatcacg ctcatagact tcgatctttc
    23161 caacaaagaa aaaccattca ttgttgatct gggaaatttg gtagcctact gccttttctt
    23221 ctttgatttt caaacgacga ggacgataat gccataacat cgtttcccga atgtaagtat
    23281 ctaaaacagc ggttggtaac tttttgctcc gtgtcaaatc atactgataa aatatttctt
    23341 cgtctactat cgaaaaagaa acaaaaaagc tgtaaaacat cattgtttcg tatacattca
    23401 gttctgctgc tgttcgacta agatgcatca cgatgattgg atcgattgct tgataaagcc
    23461 ggtccgactg atagagtaat ttcttttctt tcaaagttgc gtacgtactt ttttcattca
    23521 atagtctttt tttcgtaatc ccgagccagc aagcaatctg tgcttctgct gatgccgtaa
    23581 acgttgtttc taatacacgg tccaatccta gaatgaacgg tcttttttct ggtgtatttt
    23641 gcaaaaaaag cggtcgctga tcttccggga tatattggaa tagttcataa taaaaatatc
    23701 gtatctgtag ctcttctccg tataattgac cgtttttgat ttgtaatcca aattcctcta
    23761 agagctggtt caattctttg atctttcgaa aaacagaaga ctcactgata ttaaacgctg
    23821 tcgctaatcg aacgatggat acttgctgat gttctaataa atagacgagc atcttaaact
    23881 tcaagctttc ctgcaataaa aacaatagaa ttttttccaa acttacatct tcgtccaaca
    23941 caagcagcgc ctgatactca tttcgctgta cttctacttt tttttccatc attcggccaa
    24001 tcaattcaat atcttccaaa tattgatcaa acgaggcttt tgacagttgg acatgctctc
    24061 tcaaaccatt gatagaggct gttccccctt gcagaacaag gtttcgcaaa atcaggatct
    24121 gtaactgctc ttttctttct aataatgctt caatcttcat aggcagtatt cgccaccttt
    24181 gaccacacca tatctggcac acggtattct ggctggaaaa atagctcatg ttcttctaac
    24241 tgttcaaacg tccatccttc ttctactgca attgtcagtt ggtcaagaag gtggaacatc
    24301 tcttttttgc tttggagctg tgctccaaga atcttttttt cagaatcaaa tagaaatttg
    24361 agtttaactt tgctttgatt tattgctgta agcttgtagt ctacgtatcc tgtttcaatt
    24421 ttttggggat aaaaaatgct ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca
    24481 aaccactgat tgccgactgg acgataggtt ccctgatcct ttgtctgtga gccactaatt
    24541 gtcttgctga catttcgtgc tgtacggaca gcgttattta ctagagaaac atacattttt
    24601 tcatttgttg gacgaaaagt tacttggatc gcatcgccaa tcgcataaac gtctttttga
    24661 gacgtttgca agtagtcatc tacccagatc gttccatcat cattcaatgt taattgttct
    24721 tcccacatct gattgtcggg gcgagaatga ttagccagca aaacaagatc atagttctcc
    24781 tttctttttt ccgtctctac aatagcggtt tcattttcgg tcaaagacac aacttgttct
    24841 tctaaaaaaa gtgaaagccc ttgatttttc atttccttta ctaacggttc tgtcatttca
    24901 ggatctagat aacgaaaaag aattgacttt cggctttcgt aaagatggat ttgttttttt
    24961 cttgatgcca acccttcagc tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt
    25021 ttctttgcat tcatgatctt ttgctgaaga gccgttacgt tttgtatctt tttaactggg
    25081 tgaataagcg ttgattctgc agaaatattt cgaaactttt gatttgaacc agtcgctaga
    25141 atcaaacgat caaaatccaa tttttttcct cctgctaaaa cgactttttt ttctttcaat
    25201 cctattactg tcttttctgt gtaaacatgg attcgatagc ggtcagtcag ttcttctttc
    25261 gtgatccaag aaagttgttt ttcactggta acctttcctt ttaatatcaa attgaacccg
    25321 ctgggaatcc atcctaactt cgattgcttt tcaatcaaat atatttcact tgttggcatc
    25381 aattggcgaa gataaatagc cgcatgaatc cctgcaaacg acccgccgac aattacaatg
    25441 ttcatctgca ttcctctttc ttataaggtg ttctactgct aacttctcaa tcagaaaaca
    25501 cagtattcat aactcgcttt caaccttatg atacatagcg atgcagcaga atcaaccctc
    25561 tccatcatac cgtgttctag gtctgcttct caataatgta ctagtggacc taattgaaga
    25621 gtatcatttc tgaagagatg tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct
    25681 tttacgtacc tctgtccaaa taggaaacag aaaagaacga aaagacgagt tttagcgtgt
    25741 gtaatgtaat agttagaata gacacatcat ttatttttaa ttctttaatg cttcaacaac
    25801 tgcttcgcct aattgatgag cagattgagg attttgccct gtaagaagcc gcccatcaat
    25861 ttgaaccact tctgaaaaag ctgctccaga ctgatagttt gctccttttt ctttcaaagc
    25921 atcttctgct aagaatggta cttcattcgt tgtaccattt gcttcctctt cttcattact
    25981 gaacccagtc actgtacgtc cagctaaaat agattttcca gactcatctg taactggcaa
    26041 caatcctaca acaccgtgac aaacagctga gaccagccct tcatttcgat agatttgtga
    26101 agcgactttc ccaagatcta ttgcttctgg aaaatcccac attgttccgt ggccaccagc
    26161 ataataaacc gctgcataat catttgcatc cacatcatca ggacgtaagg tatttcccaa
    26221 ggcccgattt ctgaaatctt catcttcata ataggcccaa tcgatttcat ccatattcaa
    26281 aacactttgc ggttctaaag gtacatatcc gcctttagga ctgacaaaat ctgcatcgat
    26341 cccatttttt actaatacat cataaaaatg agtcagttca cttaaccaca aacctgtagg
    26401 acgctcgatc ttttcgtact tttctacatt tgttaatacg atcaatactt tcattttccc
    26461 atcctctttc tataaaaatt gcgttattga taacgtgctt tcaagaaatt atataatggg
    26521 cttggcaaat aaagctcttt atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca
    26581 cggttttcca ataatttctc tgcccagtca ggatcgacga tcatttgctg tccaattgct
    26641 gcaagttctg catatttcaa tacctgctct acatcttcgc gattgcggat cccaccgaca
    26701 ccaactaaag ggatctttcc ttgaatcgtg ttatggacca cttcaagaat cgatttatcg
    26761 ttgtatttat cagaacgagc tattcgatca tacatattta atgaaacatg cagataatct
    26821 agttttgact cacgtaatcg ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt
    26881 gtttcatatt cttctggtga gaaacgatac cctacgatga atggtttttt agcatattgt
    26941 tcaaccgctg agaaaaccgt tgttaataat tcttcaataa aacgataacg attttccaag
    27001 ctaccgcccc attgatcctc tcgtctattc gaatgcggag agaaaaattg ttggatcaaa
    27061 tacgtatttg ctccatgcaa ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg
    27121 gctttgccaa atgcttggat tgtttcaacg atttcttctt cactcattgc tcgtggtgtt
    27181 tccgcatcag gacgttttgc tgcaactgca cttgcagata caggctgttc tccagataaa
    27241 gttgctcgac ttgtcattcg tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct
    27301 ccttggattg ctttcgctag ctcacttaat cgaggaatca tttcatcatg agcgatgctg
    27361 agttcacctg gccagccttt tccgatatct tggacatttg cagcaccagt aataacagca
    27421 ccaagacctt tagaacgttg tttataataa gtgatctcat cttcggtgat cactccattg
    27481 aaaaagctga gttgtgtcgt cataggcgac agcatcaatc ggttcttcaa ttgaactcct
    27541 gaaggtaaag taattgattg atcaaattgt gacattgtca ttcctcccat aattcgctct
    27601 gattaaccta tcgagaatca tctatcatta aaccactcga ttagttgtac atgcaactaa
    27661 tttgcttaaa aaattttatt ttttttgtaa cttatctgtt gcatcattca ttgtctgggc
    27721 taattcttta cttgcttcat ttcccaaaac tgctttgtat gactgatgaa gttcttccca
    27781 gctaccatcg acttccttag caaattggcg tccttgttct gtaagataaa ctaatgacaa
    27841 gcgaccttcg ctaactgttt tgacgtatcc tttatattct aatttttcaa caaaacgagc
    27901 aatcgttgat ggggcggaat agagcatttg tgcaatatct ttttgtggta ttccatcttt
    27961 ttcttttaat atcaataaaa taaaaccata agttggtgcc agacctgtcc ttctaaacgc
    28021 ttcatcagct aatttattca acatgcgatc tagctttttc acgctaaaga aaagacattc
    28081 atctaacgct ttttcaattg gcttttccat ttcatcccct ccattggcgt acatatcata
    28141 gtatgatttt tattttttgt caaaaaacac ggaataatgt ccgcctcctc tgtctagatt
    28201 tgaaaaaaag aagtagccat cttcaaagaa tttcggcttg ttttcgacaa aactccttct
    28261 ggaacaccgt gaatcagagg ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt
    28321 ttgtgaaaat ggctttccac atttttgcat aaatttggct taataccgaa aaactggctt
    28381 ttgaacaccg tttatatcag aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca
    28441 gccaaacaaa aatagttcct catattttgt cgttttttga gctaatgccg atggtcagct
    28501 gattgtacac cgtttattga gaggtcgtga gcctgccgtt gaactccgag tcacaaggag
    28561 caatttcaaa aaacagcttc tcatgtttta tgactatggt gacttgtgat cgaggagttg
    28621 gcaggagaat actgtttatt cgtaaagaag gggctgtgac aagacttttg tcacagtccc
    28681 ttatttcctt ttattatagg atttcgccat ttgattggat gacttgtctg taccaatcaa
    28741 atgatttttt ctttaaccgt ttctttgtgc cattcccttc gtcatcttga tcaacataga
    28801 tatatccata acgttttgac atttgtgaag tagaagcgct gacgatatca atgcagcccc
    28861 acgacgtata accaaataaa tctactccat ctttgatagc ttccttcatc tgctcaatat
    28921 gctgtttcaa atagtcaatg cggtaatcgt cttgaacggt tccatcttct tctactttgt
    28981 ctatagctcc gagtccgttt tcagctatga acagtggttt ttgataccta tcatataatt
    29041 gattcaaagc aattctcaaa cccttaggat caatctgcca gccccattca cttgatggaa
    29101 gatacggatt tttaattcct ccagttatta agtttccttg gaccccttcc caattttctg
    29161 gatgagcact gactgcacta gacatgtagt agctaaaaga aacaaaatca acagggtagg
    29221 ctgctaagat ttcttcatca cctacttcta tttttaagtt gacacctttt tcttcaaaca
    29281 ttcgttgcgt atacgctgga taatatcctc ttgcttgaac atcactaaag aacaaagaag
    29341 ttctttgctg ctcaattgtt ttttgaatat catccggatc acaagaatac ggaacaagaa
    29401 gctgatagct gatcatacaa ccgacttgtg aacctttgat aatttcatga caagcttttg
    29461 tagctaaagc acttgctata aactgatggt gtgcaccttg gtatttcgct tgttccaaat
    29521 ggggatggtt ttcttcaatt actcctacgc taacaaaagg gtgatgtttt acacagttga
    29581 tctcattgaa agtcatccaa tattttactt tatttttata gcgtttgaat actgtttcag
    29641 cgaaacgaac gtaaaactga ataagctttc gattaggcca tccaccatat tctgtaacca
    29701 aatataacgg catttcataa tgggaaagag aaacgactgg ctcaatccca tattttagac
    29761 attcgtcaaa cacttgatca taaaaaaata atcccgcttc attgggtgtt tcttcatcac
    29821 catgaggaaa aattcttgtc catgcgatag acatccgata gcatttaaaa cccatttcag
    29881 caaataaacg gatgtcttct ttaaagtgat gatagaaatc atttccgtgt cttttaggat
    29941 aataatactt gctgttggga tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc
    30001 attgatctgt ccatttttct ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag
    30061 ataatccttt ttcatctaca tcccatgcac cttcacattg attggccgct gtcgctccgc
    30121 cccataaaaa tccttctgga aattttttca tatcgatccc cctaaacttg ctagttttct
    30181 tgtttgatta tttttcttat gactagtaat gatatttttg aatctactac tatttgtaac
    30241 aataaccatt gtggtcggat catatccctc tttgattaat tcttgcaaat ctacttccat
    30301 caataaatcg cctttacgta ctttttttcc atcagatact gcactttgaa agtatttgcc
    30361 atttaaattc actgtatcaa taccgatatg gatcagtatc tctgctcctt cttcactgac
    30421 taccccaatt gcatgtttcg ttggaaaaac tgttgaaact tccccgttta caggactgta
    30481 gatttttccg tccgttggaa tgatcgcaaa cccttctcct aaagatccac tagcaaatgt
    30541 tggatcgttt acttcagata aatcaattat ttcacctgct gcaggagtac ttaacactat
    30601 atcctttgag gattcttctt cgatttcatc cttgtagccg aaaagaaaag ttagcataca
    30661 ggaactgatc atcgctaaga aataactagc aactagccaa acaaatcctt caccaatata
    30721 aatcgggaaa gacaaaatac ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc
    30781 agctactcca ccaaatatag ctccacatat acaagcaatg taaaatggtc gtttatattt
    30841 aagagtcaca ccataaacag caggttcagt gattccaaat aatgctgtga taaatgcaga
    30901 taaagataaa ctttttactt tcttattttt cgttttcaaa aataccccaa gtactgctcc
    30961 tgcttgcgca gctacggcag tcatacatgc tggacctagc gtatcacgac cgtagaaaga
    31021 aagattgttg gtcattactg gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg
    31081 ccaaaatccg ccgataacag caccagataa aacagggcta aagttataaa cagctaaata
    31141 tccagctgca atcagctgac ttgcataatc tgcaattgga cctacaacta ataaagtaat
    31201 tgggaccatc acgatgatac aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa
    31261 gcttttgcga atcttaggct caatataaga aagaacccat acagctaaaa ttgctggaat
    31321 aaccgtactt gtataccttg ctagaatcac gggcatccct agaaatcgca aggttatgct
    31381 atcactatat gcactgacaa tcgtaggata aacgagagct gctgcaataa ccatagcgat
    31441 gaaacgatct gtattgaact tttttgcagc agtatatgcc aaaaatatag gcaagaaata
    31501 gaaaaatgca tcagctgctg cattcaaaat gatataagtt cctgattcag tagttaataa
    31561 attcaaactc gtacataagg cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc
    31621 taaagtcggg gcaaaaacag cggcgatcat gtctataaaa gagtttaaaa tgcctttttt
    31681 ctcagttcct ctctttccac tagatgcatc atcttctagt ttcatttgtt tggataatac
    31741 gtcatacact tcatttactg catttcctac tacgacttga aacattcctc cagcttcgac
    31801 tacagtgatc acaccttcta gttgctctac cttctcctta tctgccttct tattatcttt
    31861 tagggtaaag cgtaaccttg ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc
    31921 gacaaatcgg acaatatctt tcgctaattg tgaatagtcc atttttactt cccccttgtt
    31981 aattaatgcc gtagcaatcc ttcttacaag ataagtatag ttcttagaaa acggtttctc
    32041 cattcaatta gtacccctta taatagcgaa cgaaactgta caatagtgtg tatggtttac
    32101 ttagccgagc aaatcactca ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc
    32161 aaaggagaca aaaaaaacac gcttgatctt cctttgctga tgtataagtg gtatctcaag
    32221 ctgatactct gtgatttttg tttctacttg gcttttgata agacagcaca acaaagtctc
    32281 ggcatttaac ggaagcataa ctaggtcaga actaggagag attagttgct gaatggaatc
    32341 tatcttttta tggattttta tttgattttc ttctttgtag taatgatcaa gcagttttgt
    32401 tttctttttt tcttcaacta tggtcagctt ttcacgcaaa atctgataaa tctcccaaga
    32461 ttcaaactca tctagaacac taggtagata aatcaaatca tcccactcaa aaataagctc
    32521 ttgctcagta gtcaaaattg catcatattc cgttattttg tttgattgat ttttgaaaag
    32581 atacactggt tctatccggc attctttgat ccacatgttc atactttgtt gaatctgttt
    32641 ttttagtgta ttcaaaatac taattggctg gttactaaca atcagtatct ccccttgttt
    32701 tcgaaaagga ttcaacgctt cggcaagata caaaacaaaa taagcgcttt cagctaaatt
    32761 gggacgtaat tgaaaatgta ttgggaagaa acgccgaatt aaataaattt ctaaaggata
    32821 acgggagatg atcttttgta caaaatgatt catggtatta tgtccggcat ccatcctcgt
    32881 tttcatttgt ttgatatggg ttagcaaaaa ttctgtttca tggaacaata acgaaacagg
    32941 aagatctaaa aaacgaataa tcgtttgctc aaatgctctt agattcttct ctatttcttt
    33001 gtttttttca tcattaatca tcaaataatt taactctaat agtcgatttt caattgcttg
    33061 tttttcttga ttggaaaaag aatagggtag ttctgatgat aacttttttg tgagggtctc
    33121 taccatcgga ataaataatg tatacctgtt tgttttttct aaaagttctt ctcttcgaag
    33181 aatggtcaaa acgagatacc gagaaaatcg tatgaagtcc tctcctgaaa caatataatt
    33241 atatgatata agaacctctt ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat
    33301 tgtactgatt tgttgaggca accttgcgtg ttggatcacc gtttctgttc caacaagaga
    33361 taagaaaatt ctttttgaat tttcttcagc aatgattttt agtccttttg tcgacgaaac
    33421 ttccagttct aatttaggat tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga
    33481 tacggctgtt tttgaaagaa agaaaatgtc tgctatcttt tgcatactga catacccttt
    33541 ttgaaacaat aagtacgtca caatagttga agctcggaaa tccatgctgg aatagatttt
    33601 atgtttcgtc acctcttgta tgatttgctg tttagtttca gattcaattg attctaaaaa
    33661 ataacctgaa cgtttatttg actggataat gattttcttc tcgtagatag caagcatatt
    33721 tttgatttca gtttggatcg ttttcttgtt ggtacctaaa gcttcagcta attcttcgct
    33781 tgttatccaa tgattcttat ttcttaatac tacaagaatt tggagttgtc gtttatttaa
    33841 gtccatttgc ttacacctcc tctttgttat aaacatacag gaaaaacctc tcaaatgaca
    33901 atagtcactt gcagaggttt aaaaatcatt tgaagaaaga tctatattca aggtttttac
    33961 cattagactg gataatttgt ttataatatt caaaactatc ttttttcaat ctcctaaagc
    34021 ttccgctccc atcattttgt ctatccacat aaataaagcc atatcgcttc ctcatttccc
    34081 ctgttcctgc cgatacgata tcgattggtc cccaccataa gtaaccaatg atttggcaac
    34141 catcttttat tgcctcagca atttcctgaa gatgtatttt tagatactca caacgttttg
    34201 gatcgtggat tcgattcaat tcatctggtg cttcatctag tccaatgcca ttttcaacta
    34261 tgaataatgg taattcataa cggtctgtca attcattcat gacataacga aaccctagag
    34321 gatcaattgg ccaactccaa ggttcgggag atttttcctt caaaaaggga ttatcgaaac
    34381 catttgctcc gccagtatct acgcgcattt ctgcttcttt tttatagaca gcgctacgat
    34441 aataactaaa tgcaatataa tctgccgtgt tagcttgcaa gcactgttcg tctccatcta
    34501 acattactgg tgcacagtct tcttccttcc atgtcctgta aatataagct ggataatgcc
    34561 ctcggcacat gacatcggag aaaaaccatg aattgcgttt aaattgcagt gaaccaaata
    34621 cattctctgg atcacagtca tatgcataag tcgctaatgc agaaaagcta cacataatac
    34681 ccatcttagc atttggatcg attttcttta ataattgagt agcacgggcg ttcgctagaa
    34741 acatatgatg acaggcttga taaatcactt tttttgttag tgtagcaatg ggttcaagtt
    34801 tatctctagc agggaatgta accaatccag cagctgcata aggcatacga aaagcattat
    34861 tgatctcatt aaaggtcatc caatatttca ctttcccttg gtagcgttca aagacggtct
    34921 gcgcataaaa ttcaaaaaag tcgattaatt ttcgattttg ccacccccca tactctccta
    34981 ccaatgctaa gggggtttca taatgactaa gagtaattac tggctcgata tcatatcgat
    35041 ttaatgtatt gattagtctg tcataaaata cgagaccagc ctcatttggc ttctgttcgt
    35101 ctcctcttgg aaatattcgt gcccatgaga tgcttgttcg aaaagctttt aaacctaact
    35161 ccttcagtaa acgaatatcc tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa
    35221 gataatggag tgattcgtct aaagcaatct cccaaatctt tttttcttca ttccactgaa
    35281 tactaggatg ctggttcatg atcccgataa gcgtatctgt cacattaggt aatttgccgt
    35341 cttccaacca tgcaccttca cattgattag cagctactgc tccgccccat aaaaaatttt
    35401 ctggaaatat ttcctgatcc ataactatct ctcctcgcta tcaacaattt cttacttgaa
    35461 gtatagctcg attcatcaaa acaagatatt ctaactattt cggcagaaga gaagaaaaaa
    35521 attgaatgct actcttcatt tttaatattt atatataaca aatacataag acgatccccg
    35581 aaagaaaaca ctcgttaacc atctaagcaa acgatttatt ttacttccaa cacaaatgct
    35641 tcataaggtc gtaaatcaac tttgccgtct ggtaactgtt tcagaggata atttgacaca
    35701 ataaccctta aagcttcacg tggaacagtg aacgtctgct tttcactgga catattacaa
    35761 ataacgagcc attgctggtc ttcatatatg cgagaatagg caaagacatg ctcaggcgtc
    35821 ttttccacta attcatagtc tccccagacg atgatgtcat gttttctacg caaatcgatc
    35881 aatttcttgt acatataaaa aacagaatct tgatctgcta acgcttcttc tgcattgatt
    35941 tctttataat ttggattgac tcgaagccat ggaattccag tagtaaaccc agcatgttca
    36001 cgggcgttcc attgcatagg cgtcctagca ttgtctcttc ctttcgcatt gattgattcc
    36061 aagatttcct cttttgtaaa tccagaagaa atcctttcgt tatacatatt gattgtctca
    36121 atatcttttg cttccgcgat atcatcgatt gggcaattcg tcatgcctaa ctcttctcct
    36181 tgatagatat aaggggtgcc tttcatcata tggagcaaga tagcaaacat cttggcactt
    36241 tcttttcgat acacatcgtc atttccccaa cgagaaacaa ttcgcggaag atcgtgatta
    36301 ttccaaaata aactattcca tccttcatca cctaaagatg tttgccattt ggataatact
    36361 tttttcagtt cagagatact caagggtttc aaatcccatt tgtctttgcc ttcttgctga
    36421 tctaatccta tgtgttcgaa ttggaagacc atggataatt catttctttt aggattggaa
    36481 taaagtttag cgatttcggt agttgctccc catgtttcgc caacagtcat cagatttttg
    36541 tcaccaaaag ttgcttgatt catttcctga agatactcat gcagtttagg tccattactt
    36601 gtgatttctt tatccggttc tttgccaatc atatcgatca cgtccatacg aaagccccca
    36661 atacctttat cgatccagaa attcatcatt tgataaatct cttggcgaac ctgtttattt
    36721 ttccaattca aatcaggttg acgcttgcta tacaagtgca gaaaatattg ccccgttttt
    36781 tcatccaact gccaagcaga accactgaat attgagcgca atctattagg ctcttttcca
    36841 ttgaccggat cacgccaaat ataatagtcc cgataaggat tttctttatt tttacgggcc
    36901 tcaatgaacc acggatgttc atcagaagta tgattcacca ccagatccat gatgatacgg
    36961 atatttcttt tctttgcttc ttcaatcagt tcttccattt cttccatcgt accgaattct
    37021 gccatgattg cttgataatt actgatatca tacccattgt catcattcgg agactcgtat
    37081 actggagaaa gccaaattgc atcaatacct aaatacgcca gataatccaa cctggaaata
    37141 atcccccgca aatctccaat accatcttga tttgaatctt gaaaacttct tggatagatt
    37201 tgataaacaa ctgaatggtg ccaccatttt tgttccatga ttttcctcct ataaatggat
    37261 gtttctattt tttgcttaat catttatttt agtctctctc actaaaaatt gtaacgcttt
    37321 caaatagcta ttgcaccttg ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa
    37381 ttccctcaac tgttttttga aaggattgat ttatgcaggg aagaatgctg atttcacaac
    37441 ttctttataa acggtgttca ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat
    37501 atgaggaact attttttcgt ttttttctct aatactcagg ccagaacact gatttcacaa
    37561 cctctttata aacggtgttc actcagctgt ccctcggtat tagctcaaaa aacggcaaaa
    37621 tataagaaac tattttttcg tttttttctc taatactcag gccagaacgc tgatttcaca
    37681 acctctttat ttcacattca atatccacat gaatcccaca aatacaaaga acagaccata
    37741 catgattgga gatacttctt ttcctctttt agctgctaac atggtaattg gatagaagat
    37801 aaaacctaat gcaatcccat ctgaaatact ataagtcaaa ggcatgccga tcaagatcaa
    37861 gaatgaaggg atcgcaatct ctaagtttcc ccattcgatt tgtcgcaaag attgtgccat
    37921 caatactcct accataatca atgctggtgc agttacttgt gaagtaacaa ctgatagtaa
    37981 tggagaaaag aacaagccta acaagaagaa aatccctgta gtaatagctg tgatacctga
    38041 acgtcctcca acggcgatac cagctgatga ttctacaaat gcaccaacag gagaagtacc
    38101 aaataaggaa cccgcaagca tagctgttga atcagaagct aaagctttcc ctacacgagg
    38161 catcttgttg tctttcatga aacctgcttg gtttgctaat ccaactaaag ttcctgctgt
    38221 atcaaagaaa gtcacaagca agaatgtcag cactacgacc cacatttgaa gagaattgat
    38281 gtcccctaca tgttttaatg caactaaaaa tgttggtttc aaacttggtg ctgcggcaac
    38341 aatttcagaa ggcattggaa tcaaacctgt gaccagacct aaaatcgttg tagctgctat
    38401 cccgatgaag atgccacctg gtacacgccg aacaagcaag atagcagtta cgactaatcc
    38461 aaaaatcgtc aaccaagtag aaccaacgtt gagtgatcct aatcctacta atgtagattc
    38521 attcgaaacg atgatcccac cttcgctcag accaaggaaa gcgataaata gaccgatacc
    38581 gccagaaatc gcatatttca aatctgctgg gatcgcatca atgatcattt cccgcagctt
    38641 gaagattgtg atcaaaataa aaatcaaaga agcaacgaat acgccggcta atgctgtttc
    38701 ccatggaatc cccattccta aacaaacaga ataagcaaaa aaagcattga tcccaagagc
    38761 aggtgccgtt ccaattggat aacgggctaa tacccccatt aaaacacagc cgatcgcact
    38821 ggctaatgca gtcgcagtaa acactgctcc ttcatccatt ccagaagcac ctaatacagt
    38881 tggatttaca aataagatat aagccattga gataaatgtt gtaaatcctg ccagtacctc
    38941 acgttttacc gtggtgttca atttttctat ttcgaagtag gaaagtattt tttccttcat
    39001 cttccatccc cctaaatccg aacattataa ataaagtccg ttaaaatttc gatttattaa
    39061 taaaacaacg tgttttatca tatactaaaa caaaacataa gtaaacaaaa aattcggatt
    39121 ttaagcaaaa tccgaacgtt ccatctgatt aaatcccttt atctcaaagt tcatgaacat
    39181 tcactttgga ttacaagaat ttctttattg tgttaataaa gtttcttcat tctagtctca
    39241 aaaatcaata gtgtgatttg attttaccat ttcgtttctg ttcttctttg atgaattctt
    39301 ttgtatcgac aggattcaga ttatttagtc cgccaaaacc tgtatcgacc atttttcccc
    39361 agccttttgt cttctttttt cctgatttga gattctttgc tcgaagcttc tcactatgtt
    39421 ttgctcgccg atacttcaaa taaccttcta atcttcccat tagatccact gtttttcctc
    39481 ctttttattc aatcagacga ctcagctttt cctataacaa aataattgcc ttcagggtca
    39541 gaaaaattga aagtcaagat gccattgtta tctgtgattt ctcctggttc ttccatgttc
    39601 tcgtagatag tttcaaattc atccgtaaaa aatactaaag aaggcggtgg tcctaatacc
    39661 tctggtgaaa acttctgcac aaatactttt gggaatacgc ctaattctaa ttcttttgag
    39721 atgctgagta caatactctt aaattcttct ggcaattcga ttgtttcaga aatttctgca
    39781 ccaatttttt ctacaaagaa tcggcaaatc atctctacgt cattgacata aagcatcaca
    39841 cgtgtttttt gtatcatcat ttgacccttc tttctatttg attttctttc ctgtctataa
    39901 actgattcca ttatatcacg aaagcgcaaa caccttgtgt ttgatactat tcaagaaaaa
    39961 taaagcaata gtttctagga ttgctttcct ccacaaactt tttttacaaa aaaaaaaaac
    40021 ggcaatgaat gccgctcttc ttatttcgat tcttttataa ttctgcttct gattcatctt
    40081 tggttagtgt gtgtctaaca ctagttacgc caaatgtttg atcgattttt tccttcaacg
    40141 cttcgattgc ctgtgcgcca cgtgccgctt gttcagcatt gatggattca atgaccagca
    40201 ccgctgcttg tgtttcttcc gtcagcattg ttctttgggc ttcacgccag ttgagacagc
    40261 gacagatcgc tccttgttca tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg
    40321 aatctgcacc taaaggcagg aatgattcgc caccttgggc ttttcctaaa tgcagatttc
    40381 ctgcgatctt ttcgatatct tctccgccgc aaggtacgcc aaaagacaag gaaatactat
    40441 tatagatatc tacaagcgga ttgattggga aaaattcccg ttcttgattt gcacgtttca
    40501 gtaaagcttc aatagaagaa cgtgcacctt ttttagtctt gaatgtggta aatgcctgac
    40561 gccactgagc aatgacttcg ttttggctga atgtttcttc tgtaaggtaa tttcttgctt
    40621 cttttgctgc ctcattcaat aattgatgat acgtttcttc actttctttt gttacgtggt
    40681 tatcgatacc atttacggat aagatttcga tggttgcttc aggaaatact tcccagaaat
    40741 tggtatccac tattactttt ttcatctgtt cacacctctt gattagaata attgcttgat
    40801 gaatagatag tctgtttggg gatcatctcc catataaaaa acatgttcgc tgtatttgat
    40861 aaatcctttt ttacgataaa aagcgagcgc aacatcgttg tgttcccaaa caccgagcca
    40921 aatggctgtt ttttgctttt cttttgcttt ttcaatagct gtatctagta aaaatgtacc
    40981 gataccttga tgtttaaatt tttttcgtac atagattcgc tcgatttcta aaaaatgatc
    41041 agccatttcc tcagattgac tcgttcctgt atttagtttc aggtaacccg caggctcttg
    41101 atcgatactc actaggaaaa aatcggtttc tttatctttt agctcttctg taagtttttc
    41161 tagtgcataa gcttgtgtca agtaatcagc taaatgctcc ggtgaattat cttttccaaa
    41221 tgtatctgta aatgtttcga tactaatttg ttgtagtaag tctacatctg aaggcgtcat
    41281 ttttcgaatt tcgtatttat tcacggcttc ttcctttcaa tagatccttt tattgccttt
    41341 tttgacaaaa ttccaatctt cgctaacatt cttgcatact ttatttaaaa gatattccaa
    41401 ttgttttgct tcttcgtcag aaagaccatt taatgcgact gcattggagt agttgttttc
    41461 tcgaatgatg aaaggataga tttctgctcc ttttttagtc ggatagagtt tcttgatttt
    41521 tttattttct ttatcttcca aacgttcgat catcccattg gattctaatt tcttgattgc
    41581 tcgggcagtc gttgtacgat ctactttgat catttctgct agtttttctt ggatgattcc
    41641 aggattttcg caaatacgta ctaaataaag atactgtcct cttgttaaat cgacttcttt
    41701 gaattcgata ttgctaatag aatctagcgc tcgggcaatc attccgatgt ctcgaaggat
    41761 ttcagccatt tcatcccacc tttattttgt ttgtaagact agtttacctc tttttgttgc
    41821 aaatgcaact attttcaata aaaaaatcaa gttctttttt aataactagt aaagtatatt
    41881 cttttcttct tttacgaaaa agctcaatca caatagatct ggtttttgtt ttctaaaata
    41941 agtaactgtt tcttcgttgt tcgcttttct gatagctttg gcaactcttc tatactaaaa
    42001 aatcccatgt tggaagtttc attattctca ataaacttgg cctcttcacc gtggatcgca
    42061 caagcaaaaa caagtttata atactgaaaa agctgtggaa tatctttttg tttattcgta
    42121 tcaaaaactg ctcgcaactg agtagtttct actgttaatc ctgtttcttc atagacttct
    42181 ttacgcacat tttcttcagg agacaatcct atttcagcaa atccaccagg aagtgaccat
    42241 tctttcgtcc gtttatcttc gattagtagg accttgccat cttttttgat ataggcacga
    42301 acatctactt ttggtgttgg atgtccttct ccaatcgttg tgagcgcttt tatttcttcg
    42361 aaagtttctt ttcctatttc actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa
    42421 cgctctttat caaatacatc ttttccataa tatagaccag cttgagaaag tgcgagtagt
    42481 cttttgtatt gagccaactt cttgttcatt ctcttccctt cttttttagc aaatgtatga
    42541 ctatgtaatc aagtatagaa gactggttta attacggcaa ataaatataa tgaaatctgc
    42601 aataaataaa agaatgttaa aaaactcgag aacaaaagac attcgacttc tctggggctg
    42661 tgaataaatg gcgttcaaaa gcttgcatct gcggtaataa gccccaccaa acgaaaaata
    42721 tgacgagcta ttcttgtctg gctgttctta ttaattgagg caggacagct ttttcacaac
    42781 ctccccacta aacggtgttc tcctaccaac tcctcggtca caagtcacaa tattcatcaa
    42841 acatgagaag ctgttttttt gaaattgctc cttgtgactc ggagctgaac gacaggctca
    42901 cgacctctcg gtaaacagcg ttcaaaagcc aatttttcga tattaagcca tttctttgtt
    42961 acttattgtc attgatttct ttttctaatt tatccaatag tggagtgtaa tccacttgag
    43021 tatagtctgt tacatcaaat tcatatagtg ttcctaactg gaatttgtca tattttcggt
    43081 cggcgatgat ctttttgaat gcttctttcg taatatgatt ggtccctttc gttcgatctt
    43141 ccaatacgtc accatagtga taatgatcca tgatatagct caaatgacga tctgattcac
    43201 gatcacgttt gtaccgacgt tgacataaca cttcaaagtc tgctgttagg cgaatcgtga
    43261 tcacctgata tccgtatcta tctgctaatt cttggagtcg tgctttttgc ttatcactaa
    43321 aaggatactc ggacacaatc actttctttc ctgcttccat atacaaatca agtgctttgt
    43381 aatagaaagg ataaactttc gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc
    43441 cgatggattc tgcatagtat tccttgattt catcgagcgt gatggcatac atatctggaa
    43501 atcgttcccg tagtttattc ataagatatg tttttcctgt accaggacta ccagccagaa
    43561 gaatgaaata ctttttcatc tcatttattt cctaagttaa ttggactttc caagttcata
    43621 aacagtccaa tcttatgctt gacaatttct tttactgctt catttgctgg aatcacatta
    43681 tggcgaagag acttgttgat ttctgtttca ctaaaggttt ctttagctac tttcgtccag
    43741 ttgactaaaa gctccgttcc aacattgaat ttgcggatcc cgttattgat caattcagga
    43801 taatcttctt ctttcacacc tgttcctcca tggataacta gggggacatc aactactgca
    43861 tcgatttctt tcagcaaggg aatgtttact tctgtttttg atttgaattg tccatgattc
    43921 gtaccgatag caatagctaa tgcatccacg cctgtagctt ctacaaattt cactgcgtct
    43981 tccggtcttg tatatacctt gtcattttca gcgacatgga ttccttcttc tgtaccgccg
    44041 attgttccaa gttctccttc tacggaaaca ccgtgcgcat gggcatattc tactactgct
    44101 tttgtcttca ggatattttc tttgaaaggc agatgggacc catcatacat gacagaagta
    44161 tatccgctgt cgattgcttc tttgatatca tcaaagtctc gggcgtggtc taagtgcaag
    44221 actgcatcaa ccatttcttc ttctgcaata gctgtcatta cattgactaa cacatgcatg
    44281 ccgatatatt tagcggtatc gacacttgtt tgaataatga tcggcgaacc catttctttt
    44341 gctgcgccta gcatagcagg aagcatttct aggttatgcg tattaaaagc tccaactgta
    44401 aaattcaact cttctgctgt ttttgttact tctttcaatg ttgtatacat cttttattgc
    44461 caccttatct ttttatttgt ttcctttgat cgttacttgt tttgcaattt gtcgcatctc
    44521 atccattttg cccatgtagt aatcgatccc ttgatcatca ccagcacgag cagcatctgc
    44581 acgcttctgg ttatagaggc tcatcagttt tttataccca tctccgatcg tctgtttatt
    44641 ttccaaactc ttttctaaat attggatggc ttgatcgttc acatacaatt cagaaaattt
    44701 aagtccaact tgttcataaa gtttcgctaa tttatgctta tcttgggtag tggaaatctc
    44761 ttgtttcaat tgtgtaatct ctgattgaat cttttcggtc gcttctggcg atagggcttt
    44821 ttcttctttt gccagaactt ttttcggttc tttttctttt ttcttaaaaa atccaaacat
    44881 cattttccct actttcaatt aaaatgcttt caaaactggg cttaagatcc aggcatataa
    44941 gagtgctgcg gctactgtat ctacatcggt tgctgtagca ttgatgaatc ccatcgaatt
    45001 aaagatcgtt actagcaaag cgggcaataa tgtaataaag aaaccatgag caataccgcc
    45061 aatgatcgct cctcggcgtc cgccaactgc gttgccaaat atccctgctg tccctccggc
    45121 aaagaaattg gttagcatac caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa
    45181 catggcaata atcgtaccaa tcgttgtcgt aatgaaacct aaaatgacag catttgggct
    45241 ataaggaaag aaaactggac aatctaaggc agggatcgca tcaggaacta atttcatagc
    45301 tatccctcga aatgccggca cgatttcacc taacagtaga cgtacaccag ctagcaatac
    45361 atacacaccc actacgaatt ggatggcttg taaaaaggca aacatgatat agttttgatc
    45421 tcctgataat tcagaagcga atggttcgcc tgcaaataaa acagtgatga tatatagcgg
    45481 gaccatcacg accatgacag acaaataagt atcttgcaga aattcgaaat gtgtcggcaa
    45541 cttgatatct tcgatcgact ttttattttc gcctttctca ccaaaaagct tagcaacacc
    45601 ggcttcaaat aagtacccga tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt
    45661 gattttacga atgatcggtt gcgctactgc cggcatagca atggcgaaac aagctccgat
    45721 aaaaccaccg accaagatca atacgatccc tcgcagaccg gcaaaatagc caaagactgt
    45781 cgtcatcgtt gccatccata aaattgcttg accagttaaa aaaatgtact tccattttgt
    45841 gaaacgagcc aaaataatat tgaatacaaa aatggctaag aacgtcagtg caatatctct
    45901 acctaaaccc aaatcattca ttgcttgacc attgatagat tcaatagaag ggatgatccc
    45961 ttgcatatga aatccttcag taaagatttt cccgaaataa gttaaacttc caacgatgat
    46021 gctcgaaccg gcactcaaca cttgaaatcc taggagtgtc tttaacgtgc cagagatgac
    46081 ttgtcctgct gatttcttct gcagtagtag acccaacata gcaatcaatg caatggtgat
    46141 cgatgcctga gtcaaaatat tttcgatgat aaaattgata acgcccatgt ttttaccctc
    46201 ttttcttttt gtatttagat aactccctta tcttgcagaa ctggggtgat tttttcttca
    46261 atttcatttt ttgaaacaat atttttcaaa tagatcatcg ccgttttagg atcaactgcg
    46321 aacttttcga actgcgaacg aaaattttct gctgtgatga tcacatctgg tttcatggaa
    46381 actgcggaag aaatatcaca atggtccagt ttggcttcta ctcctaattt gttcaaaaca
    46441 tcctcagcag acatctgtgc tgcaaaacta cttcctaaac ctgcaccgca tacgaataaa
    46501 atgttcaatt tgttcatctt tgttctcccc cgtatcattt attatttctc aactactgaa
    46561 aaaagcagtt gtttgaaatc ttcgatattt gtgcattcaa tcaatcgatc caacttctct
    46621 tcgttgttga tcaattcaat tagttctttc atgatgttca gatgcgagta ggagtctact
    46681 gctgccaaac aaaaaataat ctttactgga tccatttcag gattaccgaa atcgattggt
    46741 tgctcaatcg tagctacgct gactcctaat ttattgaccc catcttctgg tcgggcatgg
    46801 gctagtgcca tgtgtttgcc aatcacgata tacggtccgt aatcattcac tgcacgaatc
    46861 atcgcttcga catagcgctc ttcgacaatg ttttctttaa ccaatggtct agcagctagt
    46921 tcaatcgatt cctcccaagt agtggctttt tctttgatca tgatattgct gtccgtcaaa
    46981 atatctttta acataggctg gatctccctt ttgttgattt ctaaattgtt tgtttcgaat
    47041 aatttttcta acttgccata gatgattcga tctactcggc caccactttc tttcatgaca
    47101 tctattattt gagtgaataa ctccgtcgat tcatgactgt taggctctaa ccgctgtagt
    47161 tcttcatttc ttttcaaaaa gtcttctata accaagatat tcgtctcagt taaaatcgga
    47221 tcgacaacta aaagcggctt atttcgataa ttcaattgaa aggtgctgaa aaccagattg
    47281 acatccaatt tatctactaa ttcgatttct tttgaactta aaactgcagt aatctcgatc
    47341 tgaggaaact tttctttcag attctctgct aacagatttc cagtagccat cccatggtta
    47401 cagacaacga ctgatttata aacataattc aagtctcgct tgattacact ggcgatcgtc
    47461 gaaaagtgga tgacaagaaa agctatctcg tcctccagta tcttgccacc aattacttct
    47521 tcgatcgtag gaacaaaatg agtgactgcg cggtagattg ggaaataatt tgttcggatg
    47581 ttttctttca atggattcat gacttggata tgattcttga ttcttacaat cagtgcagcc
    47641 atgtgcttat agagattttc gaataaagct tcttccttta gatggaaagg tacgtgtgtc
    47701 tcgttctcta caaattggat caattggatc gttagtaatt gagcttgtac ccactcgatt
    47761 gaattggaga tatctctagc attgaacgtc tcaatagtga aacgaagata atttctttct
    47821 acttgcggga cattgtccaa ttgaaattcc tcgatcatgt ggtcaataaa tgcagaaaag
    47881 ctactgtctt ttatttctgt atttttccaa ttaatggcag agataaattc atgtttcctg
    47941 attcgctgga tccagatcaa tgtaaaaagc aggatttgtt ttttataaat atcatcttct
    48001 tgtttcaaca acttattgga atagatctcc tgtatttttt tgatttccac caaaggaaaa
    48061 taatggagaa agattttttg agccggtgtt aattgtgcat cttgattcat aaagaagtcg
    48121 gtcattccca gattctgatt gataaaatca tagatcatcg tacggataga tcgttctgaa
    48181 cctttataaa ccagtccttg ctttccataa ctgacgatct ctatcccata actgaccagt
    48241 gttgctcgta accgtctcat atcttcatct aatgtacttt tagagatttg aaattcttct
    48301 tcttttttgt ttagatggat cgggattcgg ctgtaagcaa cggctaaaac tagatcaaaa
    48361 atacgttcgc gccgattcaa aaagccgtct ttcacatctt ttatttcagt ttctaatcga
    48421 gcttgctttt ccttttccag cttcaacaaa aaaactttgt tgcgaattgt acggatctcc
    48481 ggaagctttt tactgctcaa ccagatattc gcttcaagaa cttcctgacg gatcgtccgc
    48541 tgactgacat tgaactcact tgctagatgt tttgctttta ctggatattg ggctgtcatg
    48601 attctgtgaa gtatttcaat tgttcttgtt ttcatagaaa tgatctcctt ctgatttcaa
    48661 gtatatggtt ccgcttacat aataaaaagt agatatttct gccacttgaa gtggaaagaa
    48721 agtaagcaaa gcaaacaaaa aataagccat atgctttttc atctcctcaa cgaaaaagac
    48781 atacgcctat tattacaaaa attttgccca tctatacaaa aaagaggttg tgaaaaagct
    48841 gtcctgcatc aagtaataag aacaactaaa ctaaaatagt gcttcctatt tttcgtttgg
    48901 ttgttcttat tatcgcagat gcaagctttt aaacaccatt tagtggagag gttgtgacag
    48961 agatgttcag ccttgagcga taagaagcga ttttgaaaaa tcgcttccca tattttacaa
    49021 aatcgtgcct tattgccgat aggctatctc tggcacactg tttattcaga aaaaggagct
    49081 gcgacaaggc ttttgtcaca actccgttca taaatatttc ctgtcaatcc agcataatcg
    49141 gctgaataaa atcggcttgc catctttttt ctttctcaga aaaatcaaaa taccatatct
    49201 gagaaaaata ctgtcgttgt gtcttacttc ctgagatcaa tcgcccatct tttcgatcga
    49261 ctgaataatc acagcatctg aaatcaattc ggatggcaaa actattttcg tggatctgtc
    49321 gataatttcc tacttcctga atagttattt ttttagtatg atttcttaca ccgtctgctt
    49381 tgtttttttg cagaactttc tgatgttcgg caaacagctt gtctgtataa cagcttctgg
    49441 ctttttcagg agtttcttct tcccaagcgg attggatctt catgaatatc ttttggattt
    49501 ccagaaactg tgctttcttt tctttagccg ttcctgacat gcgggacaat atctccgctt
    49561 tcagcctttt cttttgtctt ttattcttga cgatagaaaa agcagaaacc cctcctactg
    49621 acaaaacagc taatgcaaga aatcccgttt gctcataact agaaggacct gtacctggac
    49681 cataataccc ataagaatct ctgccgtaag aacgactgcc aaaataactg cccccatatg
    49741 aacgaccacc aaatgaacct ctgcccgaac ttcgaccgct agatgtacct ccaccatgac
    49801 cacccgcgac agcgaatcct tgggtcggca gagcaaacaa acataggccc actaacaaaa
    49861 tgattgctaa ataattgatt tttttcattg tcttcctcgt tatcttttat tacaaaaaac
    49921 tatttttatg atagccttgc taaaaggaag agtcaataac cagatgcata aactgttttt
    49981 ttagcaattt tgtcttattt agtcattgag taacatcgga caaatgtatg tgtcggcagt
    50041 ccataattcc tctatcttca gcatgaacaa gcttgcagac aagagatctg cgcttcctcc
    50101 aggagagatt tggttttcct gacaaaagtg caacagtgct gtaaatggac gatttggtaa
    50161 agcactttga ctagcagcat gagcaagttt ttgcatttcc actaacgttt tctgatcact
    50221 gcgatgcaat acgcatgtgt catctagcga actcatcaaa actaacaaca tatgcatttg
    50281 tttctgtaca tatgaatcat tatctagctc cttagctgct tggattgcaa gcctgatatg
    50341 cggataccct aatatcgctt cttcatatgc accatttacg ccatattttt gcttcaccct
    50401 atgaccatga gtggtttttt tatgacgata ttttgtatca ggaaaagcag caagcttgct
    50461 gacatcatca agtattttcg ataaagaaaa aggctgttcc atactgattt tttgtgcaat
    50521 gacactgatc aatagcccca ttgcccaaat cgctccttta tgggtattga tgccatttgt
    50581 agcaatcagc attgcctttt ccgcttttcg cccaatctca gcaattcttt ctcttaactc
    50641 ctgatcaata gcatgatgcc atgctatttg agccatttct tcaaaaaaag gagttagcgt
    50701 cttagcagac ttaagaaata aagcatagtc catatcggaa tgtgcacccg catccagtgc
    50761 atccactaat ccaggttttg gacttaatgt tacttctgcg atcaatgctt cctctaccaa
    50821 cgcagcgagt tgctgagtca ctgctttttt tcgttcgtca tttatcatga cactcactcc
    50881 ttcttcctac tattacttgc tgaaaccttt tttgatagct acccaaatga tatgaagtaa
    50941 ccagcttaaa gcaaatgcac aacttgccgt cattagataa gctacaagaa tcatgactcc
    51001 tgaaggtaac tgcagccaac tatctttaaa caataataac agtacttgga aaacaaatac
    51061 attggctaaa tacgtacgat aagcataaac agacaaccat ttgaacaatg gcaattttgg
    51121 cgaatcgttt acaatcattt tggaagcgcc tatgaagaca gcaaagatga tgactaaact
    51181 gtataaactt tggatcgtat tcaagtaagg tgcattcgca aagctcaagt ctcctggatg
    51241 tgcgagcaat gaacggacag aaaagaacgc gataactaag gcaacaggta agaaaagata
    51301 acgtactctg tccaaatatt gatagacttt ttcgtgataa acaaatgcgg ctaccccata
    51361 aatcatgaaa ctagcaacaa accgatctaa taagtaccag ctgtcatgat aaggcccagt
    51421 caatacttgt gtttgataga acagatacca tgcgacatat aaaatcgttg aaccaatcaa
    51481 tactggccat acatattttt tattagcaaa tactttgtaa ccgagccaaa caaaaaacgg
    51541 catgaacaat tggatctgca gcatcataac ggtataccat aaatgaggcg ctccatcacc
    51601 tgtgacatat ttcagtaaaa agcttgcagt atccgtatac ggagtttgtt gttggactgc
    51661 tggaaatagc caaagataag ccgtcgtcca tagaagatac ggaacgacta gttcaaaaaa
    51721 cttttctttc aaaaattccc aataagacaa atgctgactt gtttttacca tattataaac
    51781 aattgcaaaa ataaacattg gtgctgtgta tttaacgatg acgtaaaagg taccaagaaa
    51841 agctgtattt gttgcgctgt ggctgtttgc caaagcaaag ctcaatatcg tttgcatcat
    51901 aacagccgtt acagctgata ctttctggaa gtcacttacg ttgattttga tttttttgtt
    51961 ttcaaacatc tctttctctc tctttttctt tattaccaaa gttctttggt taatttaggt
    52021 ccttcgactg ttttcaatag aatttttttc tctggctctc tcaaatactc ttttagtgaa
    52081 aaaccgcctt gccgagtcac tacttgggta tcgaattgct caaaaaaagc aggatgcgat
    52141 tgaattacct gaattggcaa ttcaatcggt gaatttgcgt acagcagcaa atcaaaatca
    52201 ctggtttctt ttactgccgg aatcccagtc gataattcaa atcctaagct gccgccgatt
    52261 ccccatttac atcctgataa tatgtttcta gcagcatgaa attgctgata aacaggataa
    52321 tccatgattt tctcttgtct atacgattct tgttcgatga tgtcccacgg atgaatgact
    52381 tgtttgacgg catgttttga gatttctgtt gaccatctct gattgcgggc aggtccacgt
    52441 aaaccaatcg gtagtatttc tgcgtgatcc gtcgacgcac gacgaactgt tccgaacatc
    52501 ttttcagtta gaacgaccca ttcaggcaag gtgaaccctt ctatttcatg tttatcaaaa
    52561 aggacaatat catgtgcttg aatcattttt tatgcatccc attcttcgtc cattttggca
    52621 cggactttat ttgtttcgac acgtccgaat gttttggctg tttcgtttgt gtatcgattt
    52681 gtcagcatcg tgtcatttgt attgcggaca tcttgaatgg cttcattgat tgcttcgatg
    52741 actttgctga cattttgttc tgaagcagct tggtcggtga tatcttctag gaaatgatag
    52801 atcgctccta gttttgtata attattgata tcgtaagcga tagctggaac tttttcagca
    52861 gctgcttcga tttcagcaac cgaacgtttt gtcacacgtg cagcactttc tttagacatc
    52921 gcttggacgg taatttcttt attgtttaaa gcaatcaagc ggcttgattg taatccatgt
    52981 gctaaaaatg cacctgaaat agcatttcct gcaatcaatc caataactgg atgtccagct
    53041 tgacggagtt ttgcataagc agcagcacta ttagccaaag ccacatggat accgatcaat
    53101 tcttcgttat agccgtatgc ttggcttggt acatcgatca caacgatgat tggccgtttc
    53161 acttttttgt ctttatcttc ttcgtaaacg tgatccaaaa tttgtcccat gcggaaacct
    53221 tcaactaaac caacttcacc gtttcgtaca cggtacatcg ggtttttagg atcaggtacg
    53281 attgatgtca cgatggcttc ttctatgaat ttacttgatt taccataata cacagtacta
    53341 atagaagaag ttggattttt catgccagtt agtgccgcaa accaatcgta accaacactt
    53401 gctgcagctg gttcatttcc ttctgctact tctggtaagt caattgattt tgcttgatgg
    53461 ttagcaattg cttcattgta atcgtcaatg gccattggtt ttgaaagatc taaagaatca
    53521 agtaatgata aataaaatgc tgagctttca ctgcgatgcg tgtcttttcg ttcgtccatt
    53581 gctgcgatga cagcagcttt gattgcttct acactatcag tcactaattc atcaatgatt
    53641 tttgattcta cccgttgacg agatcctaat gtattccaaa tcaagtcttt gtcactagaa
    53701 tcaaattctc gaacgcctgc ttcttgttca ataacttctg gtccattcaa accgactctc
    53761 gctttttttg ttgcgattaa ataactcatt aatgctgaag taatcgacat accgccaaat
    53821 gatcctacac gtcctggaat caaaccgatg actggtacat attgttttag agcaatgatt
    53881 acatttccga tttctgaaat agataacagt ccgtagtttg cttcttgcaa acgaacacca
    53941 cctgtatcta gtacaatgac tggaaagatc tcatttcctt ttttattttc ttctaacaca
    54001 tgttcaagtg cagcaacgat ttttgcaccg gaaacttcac cgataccgcc accttggaat
    54061 tttccttcca tagcgattac tactgcttgt ttgccattga tcgtgccacg gctgacaacg
    54121 atcccgtcat cactttcagg gacgattcct tgtgctggta aacttggcgc gatgatttga
    54181 tcgaatggat caatcaattc acgtccattg tcaagtagag cgttcactcg ttctctggcg
    54241 tttaattcta caaaactatt tttcatctga aagttcctcc attgcttgcg tcaatcgtag
    54301 gtacacaact cctggtgtag caccgaagtc attgatcacg atatctgctg aaattggata
    54361 acgatcaaaa aatctcgtta atacattgtt ccatacttcg ccgaagccat cgcttcctgt
    54421 acaaattttt acagtagatt ctgttttttc tgttggaaac atcaagattt ccaaatctcc
    54481 tgaaccgacc actcctacat ggattttctt attgatcggc tgtttcgttg gatatttata
    54541 agctaattgt tccatggatc tctccccttt ttgtaaaaca gagcatacac gttctgctta
    54601 ttcgtctttc ttcttccatc tgggtaaatg gcgacaaaag gtttttctcg ccaattttat
    54661 gaccaagttc tgaatttcat tggtggttca tacaggccat ctgaccattc gatcaaatca
    54721 tcgatggttt gtgcagctaa taatgaacgt ttcgcttcac tacgtttgat gcctagatct
    54781 tctggtaatt tcacgatgcc ggctgcacgc aagctatcta gtgattcttt cgtgctagtc
    54841 attccaagag gtgtaacacc gccaattgct gcaattgctg cttgtcgttc ttcttttgtt
    54901 tttgctttgt acaaataagc aattccttct tctgtcacga tatgtgtagt atcttcgcta
    54961 taaatcatca ctggtgcatt ggctaagcct gcttgttttt gaacagctag tgcatctaat
    55021 tcttcgacaa agactggttt tttatttgaa ccatatgttt caaccatttg aacaactaat
    55081 ttttggcctt tccctaatgg atcatcattg tcgcgaagtg attgccatgc tggtgtagaa
    55141 tgacgacgac cgccaggatt atgtcccatg ttaggcgcac cgccaaaacc agataaacgt
    55201 ccttttgtta cggtagaaga gtttccttcg taatctaatt gcaaggttga accgataaac
    55261 atatcgatgg catattgtcc tgcagcttgt gataatgttc ggttactgcg catcgtgccg
    55321 tctttaccga caaaaaagac atctgggcga gcagcaatat atttttccat accaacttcg
    55381 ccaccaaaac tatggatgct ttcaacccaa cctgattcga tggctggaat catcgttgga
    55441 tgtgggttca atacccagtt cttcgcgatt ttccctttta accctagtga ttcaccataa
    55501 gttggcagta acaattcgat agctgctgta ttgaatccaa taccgtgatt caaagattgt
    55561 acgccgtgtt ctgcgtagat tcctttgatt gccatcattg ccattagaat ttgcaattct
    55621 gtgatgtttt gaggatctcg tgtgaataaa gcttctaatt gataaggttt atctgctttc
    55681 acgacaacgt ctacccagtc accagggata tctacacgtg gtaaagaatc gacaacttcg
    55741 ttgacttgaa cgatcacgat accatctttg aaagctgcag cttcgatgat tgttggtgtt
    55801 tcttccgtat tgaaacctgt gtacaagttg ccgtctttat ctgctttatc tgcagctact
    55861 aaaacaacat ttggaatcaa atcgatgaat aagcgtccat aaagttccaa ataagtatgg
    55921 atatcaccta atttcattgt gccgtcttca atcatttggg cgacacgcaa gctttgtgca
    55981 ccagcataag agaaatcgat cttgcttgcg attccttttt caaaaatatt caaatgttct
    56041 ggtcgtgaga tactagacat gatcaaatgc agatcattca ctttttctgc atctactttt
    56101 tcaagacttt ctgaaagaaa acttgcttgt ttttgattgt cgccttctaa tacaactttg
    56161 tcgccgggag caatcagttt ttccattact tctacgattt tttcagtttc tacatacttg
    56221 ccattgagca agttgctgat tttttccaat cgctgcgctt ttttttcgcg tttagaagcc
    56281 cagttgcggt ttggtttttc cataattagt catctccatt tttgatatag aaacgtggcg
    56341 tcatcaatac catgttggtc tagattgatc gtacggatcc ctttatctac accaaatttt
    56401 gtatgaatca gctttgtcaa agtattgccg gggggaaatt caatcgtcag atccattcct
    56461 aattcttttg ccaagtccat catttggttc cactgcacag ggaaagctag gttttccaac
    56521 aaatctttac gaatctcttc tgcgtcctga gtggctcttc catccgtatt ttttatatac
    56581 aaacatctag catcagatag atggacagac tgcatatacg gcttgaattt cttgaccgta
    56641 tcattcatca agagacagtg agatggcaca gggactttca gcagcttcgc tgtttgcgct
    56701 tggtgccgtt tggcttctgc caacgtaacg gataaacctt cgatactccc agataacgta
    56761 tgttgcatcg gacaattttc atttgacaga taaactggat ggtcaggatc aaatgttttt
    56821 gctacaattt cctcagcttc gcttctcgtc acacctacta ctacgcccat tgcataaccg
    56881 cttggataag cttctttcat taagactgcg cggtgataga ctagttccgc tgcattctca
    56941 aatctcaaag atcgacaagc aacagctgca caaaaagcac caatcgaatg tcctgctgca
    57001 atatcaggct gaataccgtt tttttccatt tcatctaaat aaaatactgc ttttgtcagt
    57061 aatgctagct ggataaaaac cgtatcttga tagttttctt ctttatcaac taaccgatac
    57121 cccgtcacat tttctaaacg ttccatgtac atttcaggga tatccgctaa catccctaac
    57181 ttctgactgc cttgtccagg aaataagaaa cctgtactca tgagcctcgc ctcctcagca
    57241 cttacttttt ccactgctat cttagtcaat ttctgctata ataaaaaatg ccaaaaaaag
    57301 aaaaaaccat aacaaaaagg tatggatggg atggataatt atgaaattgc ttcatctgca
    57361 gtattttaaa aaagtattcg aaacaaaaaa tgttacccaa gcagccaaag aattatttat
    57421 ttctcaacct gccttgagcc gtgcgatcaa acatttggaa aacgaattag gtgtcccttt
    57481 gttttaccat agcggcagaa acattgagac gaccgtttat gcagaagaat tttatccata
    57541 tgcagtcaag acattagaca cattagaaga aggaatcaaa ttattgtctt cggtcaatga
    57601 gaagatcgtt acttccgtca tattgtattt agaggtagcc tctgtttcta ttccgaactt
    57661 ggtccgaatt tttatggaaa agcatcctga tatccaatta acgatcatgc aacatcattt
    57721 accagatgat attaccgatc ctgtactgta catcacttcg gagatgaagc ctggcatgac
    57781 caatattcct atcatgaaag aacctgttta tgtcgccatt cctaagaaac atcctcttgc
    57841 taaaaaagaa catctgcaat tagaagatat ccaacgcacc cccatcttga tgctgtcaaa
    57901 gagcaacgcc ttccgtcata cactggatgc agcacttgaa gcgaaaagta tcgaattgac
    57961 gatcagttcg acgacagatg atcctgcgac attacgttct attgtcaagc aaggattagc
    58021 tgttagcttt ttcccaaaag tttcttggtc ttacgataaa aatgatcctt tcgtcttgcg
    58081 ggaaatcaaa gatttgccct tgacacgaac aatttattta tcttctagct tcacagaaga
    58141 tcatcctttg atcaaaatga ttgcaaaaac gatgcgggaa ttttatttag gaaaataaaa
    58201 aaaaaagagc tgggacaaaa ttcttgtcgc agctcttttt tcgaacaaac ggtgttccag
    58261 aagtaatttc ttagaaaata agccgaaatt tcccaaaaat ttgagaagca attttcgtaa
    58321 attccttctt atttcttgaa gctaaacatt tctgtcacaa cctcttctct attgtcctag
    58381 acattcattg cgttgattta aaacttccat caatcaaatc tgctagttca tctagctttt
    58441 ctattttacg tagatcaata gagatccaat gttctttatt catgtgatag gctggaaaat
    58501 acccctcttt tcctcgcaaa attccgccaa gttcaggatc gaccttcaaa tcaagaatat
    58561 tgatgatcct atggtcactt gcatcatcat agattttttc ttctggaaca gacatcagga
    58621 ccccaaacca ttttttgctt ttttgatgtc tgaatacgca ataatcagga aattttttaa
    58681 aaatatattc tggagacaaa ttgtatgttt cattgatgtg atcgattagt tcttttttcg
    58741 ttagcatctg cttccatctc tttcttattg attcgcttta ttcaaagata ccacgtaaga
    58801 atagaaaaat gaatacaagt agttgctaat caaatacttc gatatttttc ttttttatca
    58861 actcttaaat gttcaatccg cctgtgttgg tcttgctacc atctactatt aaaatgttta
    58921 ttattgtttg cccacctatt ttttgatttt agtggtatca tttttcaagt tcatgatgaa
    58981 attcttatca tcagtaaatg ctccagaaaa ataaacaatg attgaaaaat cagttgaaac
    59041 ttgaccaccg tttattttct ccgtattctt agaatagaag ggagtctgat tgatgtattc
    59101 actaatgaaa aaaatcatta ccgaaaaaga tattcgacgt catgtttctt tagtagaaca
    59161 attgctgaat catacaaaga taaccgtaaa tgaattagct gaaatcatag ggacaaccga
    59221 acgaacgatt ttttctgacc ttcaatctat tcgctctcag ctgcccgaag gctgggacat
    59281 catcagcgat caagcaggaa tcagtttgca aaaccagcaa aatttattaa caaatgacct
    59341 ttgggagatt tttttcaaac aatcagtcag tgtcgaactg ctgaaaaatc tgctttttac
    59401 aaaaaaagtt gctgtccctg attttctagc cgaccatggg ctttcttatg ggacattgaa
    59461 aagacatgtg actaaaatca atcagcgttt agcaagttac gacttgcaga tcgatttgac
    59521 gaaatataca gcttgtattc ttggaaaaga gcgtgcgatt cgtacttttt atcatcgatt
    59581 gctgattcct ttcactcata acaactattt tttcgaggat tattcgatcc atgaatccca
    59641 ttattttcaa tttttaagaa acctcagtca aactgaacta gcggttgaaa cagaagagat
    59701 tttcggaaca tgttggttct ttatcaatac gatccgtata aaagcgaatt gccgattaga
    59761 ttcttctatc cataccaaca gcacactctc ctccctgtat gactctgcat tgaaaaaatt
    59821 gtatttaacg gaaggtattt atcttaaaga cactgagctt tcttttgctt ctttctgctt
    59881 tctggaaagt tggaattaca acaataacta tggacaagaa accgctcgct gcctgcatca
    59941 ttctccattt ttagaggtcc tcgaaacgtt tgtagaggaa ctagcttcag agctgtctct
    60001 tgatcaacta aaaaaaacat ctctgactga taacttggct ctcctcatat taaaatatca
    60061 tgaatcgccg atattgattg aagcattgga ccgtcaatac catcgttttt tagaaacata
    60121 tgatcagcaa tatgcacact tgtatcaaca gaaagagaat ttactgaacg atctaaaaaa
    60181 agaaatagaa atcaatgaac caaattactt tctccaactc ttgtctctac tgatccaaca
    60241 aacgattttt tctatcaaac cgaatttgtt caatgtctat tttattttcc aaggagaacc
    60301 ttcatggaag gcgttcttgc agcaggaatt aaaagattac ttaggaaaac gtgtctgctt
    60361 cttacctatc gaactaaccg atctttccac tatttctttt gaaaaaacag atatcttgat
    60421 ttccaatttc cctctagatt ctctagatat ccctattgta tacatttctt ctattccaac
    60481 gaaaaatgag ctcaatcgtt tgactgaact cactttcaaa acttttttat agtaacggct
    60541 agcgactgta ttctctagct gttttttctt ttttgcgaaa cagtttgttc atgatccctt
    60601 tttctatttc tacaactaag aatagaagaa tcacatttgc tccaatcaat aactgttgtg
    60661 tcatcgttaa acttactgta ccgatcagct gctggccgac tggtagaaaa ataaccatcc
    60721 cttgcaataa agcaaggata cctaatgaaa gaaaaagcgc tttgttttgc aaaagacctt
    60781 ggttcaatga cggcttggac aactctctgc aattgatcat gtaaacagct tgagctaaga
    60841 cgatattttg cagaagaatg gtttgctgta agctttgccc ttcaaactgc atagccaata
    60901 aataggcagg aaccatgatc agtagggaga catagatgat ccgaaacaaa tgataaccag
    60961 ataggattcc ttgctttgga tctcttggtg ggcgcttcat gatttctgga tctggcgact
    61021 cgaaccctag tgcataagat aaagtgattg tcgttaccat attgacccat aaaatctgta
    61081 ctggggacaa aggtagcggg cgattcagca taagtgccca aatcaagatc aatccttgag
    61141 ctaatgcagt tggcaagaag aaattgatgg ttttttgcaa attatccaaa atccgccggc
    61201 cctcttttac ggcttttgca atcgtatgga aattatcatc tcctaaaacc atatcagctg
    61261 cctgtttgct tacttcacta cctttgattc ccatagcaat tcctacatcg gatcttttca
    61321 aagcaggtgc atcatttacg ccgtctccgg tcattccgac gatttctcca tttttctgca
    61381 aagctttaac aattcgcagt ttatgttctg gtgtcgttcg agcaaagaca tcgactattg
    61441 gcacttgctg aatcaactct tcatcagaca taagatccaa atcgatccct tctaaaactt
    61501 tggcagtatg ttttaagcca atttgttccg caatcgcttt tgctgtgtct ttgtgatccc
    61561 ctgtaatcat tttcactgag atccctgctt gttggcattc ctttacagct ttgactgcac
    61621 tttcttttgg cggatcgatc aatccggcga tccctgcaaa agttaattgg gttaaagtct
    61681 catgcgtgag ttcctgtttc gaatctactt ttttataagc aaatcctaaa acacgctgac
    61741 cttttctcgc aaattctgca gctgtctgac tccatgcttc ttgttctgca gaagaaagag
    61801 ttgatttagc aaataatact tctggtgcac ctttgacgta aatcaccgct cctttttcct
    61861 ccgcatgcat cgttgccata tatttgtatt cagaactgaa cggaatcttt gcttctattt
    61921 ttttcaaagg tagattggct gtttctgtgt gtcgaatcag cgcaacttct gtcggattgc
    61981 cacttaaatt ttctgttttc tgttcttctt ttgtttttaa ttcctgacag tttttcataa
    62041 tggacaacac gcaggcttct tcttttgcag ctacatccac aactgtcatt tcattttttg
    62101 tcaatgttcc tgtcttatct gaacagatca ctgtcataga acctaatgtt tctaccgaag
    62161 gaagtccttt gatgatcgcc ttttcttttg ccatttcatg tactccaacg gatagaatca
    62221 tggtcaaaac agctggcaat ccttcgggta ccattgacac caccaaagca atcgtagccg
    62281 aaagaaggat atgccaatcc ataccatatc gaaatgtcgt aaaaataact aagaataggc
    62341 tcaaacaaag aatgccttga aaaatttgtt tgtttaatcg gttgattttt ttaatcagag
    62401 gcgtcgtttg ttgttcgacc gattgcaatg cttgattgat tttaccgatt tctgtcgtgt
    62461 ttcctgtttc tacaacgaca ccgactgcag aaccagattg tacaagcgtt cctgaaaaag
    62521 ccatattttt ctggtcgccg atcgattctt cttctgtcaa aacggcatga cttttttcag
    62581 atgcttctga ttctcccgtc aaaatcgatt cttccactaa taaatcatgt gcttcgatta
    62641 atcggatatc tgctggaacg acatctccgg cacgtaagac aatgacatct ccttttacca
    62701 gttctttcgc agggattttt tcttgcatcc cgtttgatag tactatcgct tcttgtccca
    62761 tcagctgctt caaaccattc aatgattctt ccgcttttct ttcttgccag taactaacga
    62821 gtccattgac gatcactacg aataaaataa tacttccttc aatatattca ctagaagcaa
    62881 atttcaaaaa agaggcaatg atcaacacga tcatcagcag atcagtaaaa tgctttgcta
    62941 tttttttcca tcgttttgtc tgctgccttg cttttatttc attatatcct tgatcagtca
    63001 ggcgtttctg tcgttcttct tgcgacaagc cttctgttga tgtttgatag tttttcaata
    63061 catcttctac cgtcaatctg taccagttca tcctatcccc tcctgtttaa ttgatactgt
    63121 tattatacgc agggaagttt tttcaggcgt atcgattatt tcaggggggt ctgaaataat
    63181 tcgttttgca aaatgtatct gggcctaact gatatttcta ttgcttattc ttttatatac
    63241 aaaatttaag aatacaaacc agataaccaa taaaaaagga ataaagcgtg tcgtgctttc
    63301 gaataacaag ctgaaagcaa ttgaagtaaa aaacaccaac aaagcaagat ctttatatct
    63361 ttgtgtagtg ttccgtttct tttctttaat aaattgaaca tgtgcacaca agatcgaaga
    63421 ccacacaaat agaaaacaac tcgttgtcat gcctgccaaa agctgaaaaa acaatcgact
    63481 atcagctaca aaaaaatgta gacaaagacc ggcaaaaaat aacattcctg taattaaaat
    63541 accgtgtaaa ggtacgcctt tcttagaaag cgcacaaaac caatggggca aacttttttc
    63601 ttttgctgtt tgatataaaa tcctgctagt actgtagact gcactatttg ctgaggacaa
    63661 agaagcaacc aaaatcatta gattgacaat tgctgctccg ccttggcaat ggatcacttg
    63721 cagcatagtc actaaaggac tttcttttaa atctaaacaa tgccatggaa caacaaataa
    63781 aatcgacaaa atcgttccta tatagaaaaa gagaatacgc caaggcagac agttgattgc
    63841 ttttttcaaa ttcttttgcg gatcacttgt ctcgccaacg gttaagccga tcatttcaat
    63901 tccagcaaaa ctgaataaaa ccataggaaa tgagactacc attccagcaa agccaaacgg
    63961 tagtaattca tcgatctgtc caaaattttc tttgattccg atagactggg tatgaaaatc
    64021 tgagacactc aatcctatac ccaataagat aaaaaggaaa atccccaaga tcttgatgat
    64081 tgagaaaaaa gattccaatt caccaaacca cttgactgca acaaggttga ctgccattaa
    64141 gatcattagt gcgctacctc cgcttatcaa agctggtaca tgtacaaacc agaaatggat
    64201 atacaatcct attgctgtta attccgcaag tccagctcca agccagcaaa gccaataagt
    64261 ccaattgaca aaaaatgccc actttttgcc taaataatag tcaacgaaat cactgataga
    64321 gttaaatgta ctatttgcta aaaataaatc acccatggcc cgcatcaaac cataaatagc
    64381 caaaccgcat atcaaataaa cgagaataac agctggccct gctgatgcaa ttgcctttgc
    64441 tgaacctaaa aataatcctg ttccaattgc gccacctaat gcgattaact ggatatttcg
    64501 attagttaga gtccgtttca ttgccggttt ttccatcaag ttcacctttt tctttttcaa
    64561 gcagcatggc gacttcctgt tcccaagaag gtttctttat tttttcaata cagagtggta
    64621 ctacaaacaa aatgacactg atggctgtga ctgcaattag attgatccat gttccagctt
    64681 tcatcgtcag tccgacagaa aatccgattg ccgcaaataa aacaataacg acagtccaaa
    64741 ctccaagatt gctctttttc tctttttttc caattctaaa tggtcgattc aactttgcat
    64801 gtgtataacg caatctcaaa actgcgatcc ccatgatgac atagacgata cagtagataa
    64861 cagtagttga atttacgagc atcaaaaatg cttgattgac gccaggaatc agcaaataaa
    64921 aaagtgcaaa gattgagata atgacagact gagtcaaaat aacggatttg gatacgtcaa
    64981 attgattcgt cttccaaaaa ttgaattttg gtggatacgc accttttcta gcactttctg
    65041 tcactgtctt tgctggtcct gaggcccaag cagataattg gactgctaca ccaatgaaga
    65101 caagtaaact aaacagattc acaataagat gtggcaaacc aagaatttgg caataaatcg
    65161 aaatagattg cgcgatatta ttcaattcca tttgtccttt tggtacgaca tttgctacaa
    65221 ctaaagcatt caaaatattg acgaaaaata taaaaattaa cgcggcaaat actccgcgaa
    65281 tatacgtttt aactggattt tccaatcgag tgatatacac agaggacatt tcgataccag
    65341 taaaaataaa catgatcggc gcaaaataaa caaatgagga tgcagtcgta gtatctggta
    65401 ttaatttgtc ccatgaaaaa ctgcctaatg ttccggttgg tacgatccca actttgatcc
    65461 acgcagcaaa ccctaataag agcatcatcg tcaaaggcac gtacaatcct aaccaaacac
    65521 cgacttttcc gccgattttt gccatatcaa atttcaaatt caaaagagtg atgatccaat
    65581 aaacgactaa aatgacaact aatgtaaatt tgctgttcaa accaagcggc acattatcga
    65641 taatattacc aaacaacgga gcgagtgctg aagcgaccat caccatccca ggaaacattt
    65701 ggacccataa cagccatgag acaacgaacc cccatttttt tcctagagca ttcgataccc
    65761 aaagttctgg accacctttt tggggaaaca ttccagcaaa ttcaccagag atcaatgaga
    65821 ttggaaaagc gtacaataat actgcaacca gcatgtaaaa aatcatcgtc cagctcgttg
    65881 ctgctacatc tggaatattt cgtacacttg ctacaagggc gcaggtcatt ccgataaagg
    65941 tcatggtcgt gatttgcttt gatgaaccag tatctttcat ctttattcct tctttcaata
    66001 gcgacatatg tttttgtttt cttttatttt tgttcaagaa cagaaatgac aaggtttcat
    66061 cgattcagta caaaacccag tatcaccgaa acatccattg aacgatctta gtgaactggg
    66121 tttgatacat gaaacgaacg atccaactta tagaacgttt tataaaaaag aatatcttgg
    66181 cgaggaatat atgaaaaaca gggggaaata caattgtttt gatgaagcaa ataattgtct
    66241 gttctccata tgtctcttgt tattctttca cacaaaaatc agttctattg gatgatttcg
    66301 tttagtttct tttccattgc tttggcgatt ttttttgtat aaaaattgaa aattcgatca
    66361 tcattcaaat aaggagtcat gatagccgca cgtagcagag tgattttctg ttcttttttc
    66421 cattcttctt tcgttagacc cattctttca acaaaacgaa tcggagaatc accgtaatct
    66481 tcttgtgtaa atgtagtatg agaagtaata aaccgttcac cataaacatc gccatccata
    66541 taagaagacc gatcgaacat tttttcattt aattcattga tagcgttcaa atctgtacag
    66601 ccttgttctt tgaataccca attgaccata ttaaaatcag gatgattcaa tggatagact
    66661 tcgattgttt ttcctttaac agtaaaagtc agatgatcga gaaattctct gaatctttga
    66721 gctgcctcga ttgaagcccc aatcaattga ccatatccgg tcacattcaa tggcaataca
    66781 cgatgtgctg tccagactgc ggcggcggtt gctccagctt ttgatccttc caaaatataa
    66841 gctcctaaca tatctggagc ctttactgat ttttcaaaca catatggcgc gaaataagaa
    66901 atgatatttc tcatattttt atgtttgatt acgattcctc cagcagcata agggacatag
    66961 cccatcttat gaggatctat cgtgacagaa tccgcttctg aaatagctct gaacccttca
    67021 tatacctctt tgtcaatcgt tacgcagtgg tggaaaacat gatgttcttc aaagaattca
    67081 gctaaggaag catacggcac aaattctcct gcttcgttta gaaacagtga acgcgcatag
    67141 ccgccatatg cggcatcaac atgtaaatag aaatagatcc cttcatcttt taacctttct
    67201 cttaattgga cgattttatc tacactgtct acttgacctt cttctgtcgt tcctacgaca
    67261 gcaaccacac ctagaattgg gattttttgt ccagctaatt cacgaattgt tttttccaaa
    67321 gcattaatat ccattcgata atcctcttga actgggatag caaccatctg atctaagccg
    67381 actccgcaaa tatctaacgc cttcatccaa gaatagtgtt ttgtttgagg gaccagccat
    67441 ttccctaatc tttggatatt ttttccgcta cgggaagaag ctgctttgac ctcatccatt
    67501 tcttcgtccg taaagctttc ggtcatttcc aaaatttctt caacagataa attcaaaagt
    67561 tcccactcag acattttttt cactttttca ggcagcactt ccttgacagc taaaggaatc
    67621 gatttgatgc aacgggcgta ccataatcct tcaagattag caatagaacc atctgctgca
    67681 atgtgtcccc atccgtctgt catattgaat aatgaagcaa aatcctgtcc tacttctgct
    67741 tccatttgcg aagttgccat agaagattcc aatgcaacat tatttgggtt ccacagcatc
    67801 gcatagttgt aagctaataa agcaggcatc aatgtttcag cattcatttg tccccagtaa
    67861 cggcctgctg aatgccatgg gatcgatcct gcgcgtaatc gctgactgac ttcatccaag
    67921 acctctatca tatggtcgcg ggtggctaga taatctggag tgagcttatc tccttcgcta
    67981 attgcaggca tatctgaagg aatataattt tctctccatc caagatgttc atctaccatc
    68041 ttgttcaaca acattttata aactggtcca ttttcagctt tgtcgcctat aaagacggcc
    68101 ttgatatcca tatctttcat ttttcttccc tccatatttt gttggtcaat gtcttgcaga
    68161 tacatgtgtt tatctgtaac acctcttcac tatagcgcca aaaatgagaa attttttttt
    68221 ataacatcaa atccacgtag atttttacaa acaaaaataa ataaagtcgg atttttctga
    68281 taattcaacg caaaaaagcg aacaattttt caataaatca gtcttctaaa atagataacc
    68341 taaaaaaagc gaacaaacaa ataaaatgac tgtccttcat tcac
//
LOCUS       DAZULO010000007        60536 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000007 DAZULO010000000
VERSION     DAZULO010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.007"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..1535)
                     /locus_tag="ACG8OA_000463"
     CDS             complement(12..1535)
                     /locus_tag="ACG8OA_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS4975307.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="ACG8OA_000464"
     CDS             1787..2968
                     /locus_tag="ACG8OA_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4975308.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="ACG8OA_000465"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="ACG8OA_000465"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFS4975309.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="ACG8OA_000466"
     CDS             complement(5053..7764)
                     /locus_tag="ACG8OA_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFS4975310.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="ACG8OA_000467"
     CDS             complement(7774..8475)
                     /locus_tag="ACG8OA_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4975311.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="ACG8OA_000468"
     CDS             8661..9260
                     /locus_tag="ACG8OA_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS4975312.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="ACG8OA_000469"
     CDS             9341..10354
                     /locus_tag="ACG8OA_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFS4975313.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="ACG8OA_000470"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="ACG8OA_000470"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFS4975314.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="ACG8OA_000471"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="ACG8OA_000471"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFS4975315.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="ACG8OA_000472"
     CDS             11802..13175
                     /locus_tag="ACG8OA_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4975316.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="ACG8OA_000473"
     CDS             13532..15676
                     /locus_tag="ACG8OA_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4975317.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(15728..16768)
                     /locus_tag="ACG8OA_000474"
     CDS             complement(15728..16768)
                     /locus_tag="ACG8OA_000474"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS4975318.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17045..18847)
                     /locus_tag="ACG8OA_000475"
     CDS             complement(17045..18847)
                     /locus_tag="ACG8OA_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4975319.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18861..19613)
                     /locus_tag="ACG8OA_000476"
     CDS             complement(18861..19613)
                     /locus_tag="ACG8OA_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4975320.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19622..19984)
                     /locus_tag="ACG8OA_000477"
     CDS             complement(19622..19984)
                     /locus_tag="ACG8OA_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4975321.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20160..21032)
                     /locus_tag="ACG8OA_000478"
     CDS             complement(20160..21032)
                     /locus_tag="ACG8OA_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS4975322.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21058..21570)
                     /locus_tag="ACG8OA_000479"
     CDS             complement(21058..21570)
                     /locus_tag="ACG8OA_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFS4975323.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(21708..22499)
                     /locus_tag="ACG8OA_000480"
     CDS             complement(21708..22499)
                     /locus_tag="ACG8OA_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFS4975324.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22554..23162)
                     /locus_tag="ACG8OA_000481"
     CDS             complement(22554..23162)
                     /locus_tag="ACG8OA_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFS4975325.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23155..23472)
                     /locus_tag="ACG8OA_000482"
     CDS             complement(23155..23472)
                     /locus_tag="ACG8OA_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4975326.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23628..24356)
                     /locus_tag="ACG8OA_000483"
     CDS             complement(23628..24356)
                     /locus_tag="ACG8OA_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFS4975327.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24515..25396
                     /locus_tag="ACG8OA_000484"
     CDS             24515..25396
                     /locus_tag="ACG8OA_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4975328.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25633..26676)
                     /locus_tag="ACG8OA_000485"
     CDS             complement(25633..26676)
                     /locus_tag="ACG8OA_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4975329.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26679..27347)
                     /locus_tag="ACG8OA_000486"
     CDS             complement(26679..27347)
                     /locus_tag="ACG8OA_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4975330.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27489..29825
                     /locus_tag="ACG8OA_000487"
     CDS             27489..29825
                     /locus_tag="ACG8OA_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFS4975331.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29940..30251)
                     /locus_tag="ACG8OA_000488"
     CDS             complement(29940..30251)
                     /locus_tag="ACG8OA_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975332.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30432..31907
                     /locus_tag="ACG8OA_000489"
     CDS             30432..31907
                     /locus_tag="ACG8OA_000489"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFS4975333.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRNIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31954..32538
                     /locus_tag="ACG8OA_000490"
     CDS             31954..32538
                     /locus_tag="ACG8OA_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFS4975334.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            32540..32959
                     /locus_tag="ACG8OA_000491"
     CDS             32540..32959
                     /locus_tag="ACG8OA_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFS4975335.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(33240..33524)
                     /locus_tag="ACG8OA_000492"
     CDS             complement(33240..33524)
                     /locus_tag="ACG8OA_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFS4975336.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33876..34586)
                     /locus_tag="ACG8OA_000493"
     CDS             complement(33876..34586)
                     /locus_tag="ACG8OA_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFS4975337.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34583..36055)
                     /locus_tag="ACG8OA_000494"
     CDS             complement(34583..36055)
                     /locus_tag="ACG8OA_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS4975338.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36193..36666
                     /locus_tag="ACG8OA_000495"
     CDS             36193..36666
                     /locus_tag="ACG8OA_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4975339.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36819..37370)
                     /locus_tag="ACG8OA_000496"
     CDS             complement(36819..37370)
                     /locus_tag="ACG8OA_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4975340.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37475..38005
                     /locus_tag="ACG8OA_000497"
     CDS             37475..38005
                     /locus_tag="ACG8OA_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFS4975341.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38488..38931)
                     /locus_tag="ACG8OA_000498"
     CDS             complement(38488..38931)
                     /locus_tag="ACG8OA_000498"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4975342.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38947..40107)
                     /locus_tag="ACG8OA_000499"
     CDS             complement(38947..40107)
                     /locus_tag="ACG8OA_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4975343.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40614..42314
                     /locus_tag="ACG8OA_000500"
     CDS             40614..42314
                     /locus_tag="ACG8OA_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975344.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42416..43249)
                     /locus_tag="ACG8OA_000501"
     CDS             complement(42416..43249)
                     /locus_tag="ACG8OA_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFS4975345.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43553..45718)
                     /locus_tag="ACG8OA_000502"
     CDS             complement(43553..45718)
                     /locus_tag="ACG8OA_000502"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFS4975346.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45978..48272
                     /locus_tag="ACG8OA_000503"
     CDS             45978..48272
                     /locus_tag="ACG8OA_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFS4975347.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48265..48930
                     /gene="pgmB"
                     /locus_tag="ACG8OA_000504"
     CDS             48265..48930
                     /gene="pgmB"
                     /locus_tag="ACG8OA_000504"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS4975348.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            48923..49930
                     /locus_tag="ACG8OA_000505"
     CDS             48923..49930
                     /locus_tag="ACG8OA_000505"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS4975349.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49952..50971
                     /locus_tag="ACG8OA_000506"
     CDS             49952..50971
                     /locus_tag="ACG8OA_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4975350.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="ACG8OA_000507"
     CDS             complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="ACG8OA_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS4975351.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52729..54150)
                     /gene="tyrP"
                     /locus_tag="ACG8OA_000508"
     CDS             complement(52729..54150)
                     /gene="tyrP"
                     /locus_tag="ACG8OA_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFS4975352.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="ACG8OA_000509"
     CDS             complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="ACG8OA_000509"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS4975353.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56561..57817)
                     /gene="tyrS"
                     /locus_tag="ACG8OA_000510"
     CDS             complement(56561..57817)
                     /gene="tyrS"
                     /locus_tag="ACG8OA_000510"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFS4975354.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57929..58134)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58397..59698
                     /locus_tag="ACG8OA_000511"
     CDS             58397..59698
                     /locus_tag="ACG8OA_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS4975355.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59767..60366)
                     /locus_tag="ACG8OA_000512"
     CDS             complement(59767..60366)
                     /locus_tag="ACG8OA_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4975356.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        18278 a        12374 c        10842 g        19042 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15721 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15781 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15841 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15901 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15961 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    16021 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16081 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16141 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16201 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16261 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16321 gcgtataccg tacttctaca tctaaagttc caggaaatcc gtttcccata tctgggctag
    16381 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16441 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16501 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16561 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16621 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16681 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16741 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16801 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16861 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16921 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16981 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    17041 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17101 agatgaaata caatacgaag aagatcgtaa acggaaccca aatatctttg tatggatttt
    17161 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17221 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17281 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17341 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17401 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17461 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17521 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17581 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17641 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17701 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17761 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17821 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17881 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17941 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    18001 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18061 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18121 tgaaataagt tcctaatatg atcgtaacta aggctacccc tataccaaag acttgatagg
    18181 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18241 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18301 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18361 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18421 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18481 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18541 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18601 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18661 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18721 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18781 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18841 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18901 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18961 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    19021 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19081 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19141 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19201 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19261 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19321 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19381 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19441 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19501 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19561 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19621 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19681 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19741 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19801 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19861 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19921 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19981 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    20041 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20101 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20161 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20221 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20281 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20341 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20401 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20461 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20521 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20581 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20641 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20701 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20761 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20821 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20881 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20941 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    21001 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21061 ataaatatct aatgttactt ttttaatttt ttttcgaata cgcgccatta caccttctag
    21121 ttctaactct ttgtcttcag tgatgtacca accccttgcc tgtgtccctc cggctgcctg
    21181 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    21241 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    21301 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    21361 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    21421 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    21481 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    21541 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    21601 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    21661 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    21721 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    21781 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    21841 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    21901 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    21961 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    22021 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    22081 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    22141 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    22201 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    22261 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    22321 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    22381 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    22441 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    22501 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    22561 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    22621 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    22681 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    22741 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    22801 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    22861 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    22921 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    22981 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    23041 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    23101 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    23161 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    23221 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    23281 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    23341 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    23401 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    23461 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    23521 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    23581 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    23641 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    23701 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    23761 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    23821 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    23881 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    23941 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    24001 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    24061 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    24121 atcccattac ctaaatgaac aatcagacca atcgctgttc ctatcactaa agcaaaaata
    24181 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    24241 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    24301 ccaattagtc cgccaagcaa aacggaacct acattgatca aaactccagt tggcatcaaa
    24361 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    24421 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    24481 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    24541 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    24601 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    24661 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    24721 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    24781 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    24841 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    24901 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaaa
    24961 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    25021 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    25081 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    25141 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    25201 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    25261 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    25321 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    25381 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    25441 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    25501 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    25561 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    25621 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    25681 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    25741 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    25801 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagtc caatctctgt
    25861 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    25921 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    25981 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgca aaaaaacgat
    26041 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26101 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26161 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26221 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26281 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26341 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26401 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26461 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26521 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26581 aataaaaccg agaaaacaca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26641 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26701 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26761 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26821 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26881 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26941 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    27001 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27061 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27121 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27181 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27241 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27301 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27361 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27421 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27481 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27541 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27601 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27661 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27721 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27781 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27841 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacagatcat atgcataaaa
    27901 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27961 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    28021 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28081 ataatcctga actggcccat caatacgcag atcggatcat tgaattttct gatggtaaga
    28141 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28201 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28261 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28321 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28381 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28441 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28501 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28561 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28621 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28681 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28741 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28801 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28861 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28921 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28981 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    29041 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29101 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29161 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29221 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29281 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29341 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29401 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29461 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29521 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29581 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29641 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29701 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29761 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29821 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29881 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29941 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    30001 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30061 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30121 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30181 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactccttc
    30241 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30301 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30361 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30421 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30481 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30541 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30601 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30661 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30721 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30781 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30841 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30901 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30961 atacgatcgg tatgtacatc atgcctggcg gacgcaacat tggtcacttc caaaaacaat
    31021 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31081 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31141 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31201 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31261 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31321 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31381 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31441 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31501 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31561 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31621 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31681 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31741 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31801 tgagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31861 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31921 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31981 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    32041 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32101 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32161 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32221 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32281 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32341 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32401 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32461 gttaatcacc atacaatgaa acttttggat caatttggaa ttgtaaatga gcattcaaaa
    32521 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32581 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32641 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32701 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32761 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32821 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32881 gattttagat tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga
    32941 tggtgaaaaa acattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    33001 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33061 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33121 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33181 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33241 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33301 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33361 ttcacgatac tgccttcata cggcatattg tcaaaagttg cttggacttt aactcttcca
    33421 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgtcgact
    33481 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33541 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33601 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33661 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33721 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33781 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33841 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33901 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33961 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    34021 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34081 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34141 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34201 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34261 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34321 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34381 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34441 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34501 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34561 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34621 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34681 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34741 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34801 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34861 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34921 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34981 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    35041 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35101 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35161 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35221 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35281 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35341 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35401 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35461 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35521 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35581 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35641 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacgc
    35701 cgcaagcttg gatcaagcga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35761 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35821 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35881 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35941 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    36001 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36061 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36121 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36181 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36241 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36301 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36361 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36421 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36481 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36541 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36601 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36661 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36721 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36781 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36841 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36901 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36961 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    37021 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37081 ttaaccggta gttcattggt tggtgtatga atccactctg ccaattgttc cttcccttct
    37141 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37201 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37261 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37321 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37381 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37441 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37501 ttttattgga actcacttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37561 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37621 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37681 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37741 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37801 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37861 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37921 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37981 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    38041 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38101 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38161 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38221 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38281 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38341 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38401 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38461 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38521 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38581 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38641 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38701 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38761 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38821 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38881 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38941 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    39001 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39061 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39121 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39181 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39241 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39301 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39361 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39421 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39481 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39541 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39601 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39661 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39721 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39781 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39841 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39901 ttttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39961 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    40021 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40081 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40141 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40201 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40261 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40321 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aacagcttct catattttct
    40381 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40441 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40501 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40561 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40621 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40681 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40741 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40801 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40861 ccgtttacgg cccatttttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40921 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40981 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    41041 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41101 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41161 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41221 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41281 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41341 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41401 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41461 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41521 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41581 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41641 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41701 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41761 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41821 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41881 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41941 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    42001 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42061 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42121 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagaaaa
    42181 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42241 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42301 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42361 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42421 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42481 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42541 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42601 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42661 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42721 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42781 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42841 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgagattgg gagacggtaa
    42901 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42961 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    43021 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt acctgacaga
    43081 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43141 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43201 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43261 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43321 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43381 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43441 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43501 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43561 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43621 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43681 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43741 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43801 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43861 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43921 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43981 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    44041 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44101 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44161 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44221 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44281 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44341 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44401 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44461 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44521 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44581 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44641 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44701 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44761 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44821 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44881 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44941 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    45001 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45061 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45121 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45181 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45241 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45301 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45361 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45421 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45481 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45541 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45601 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45661 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45721 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45781 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45841 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45901 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45961 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    46021 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46081 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46141 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46201 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46261 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46321 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46381 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46441 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46501 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46561 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46621 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46681 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46741 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46801 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46861 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46921 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46981 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    47041 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47101 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47161 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47221 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47281 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47341 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47401 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47461 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47521 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47581 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47641 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47701 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47761 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47821 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47881 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47941 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    48001 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48061 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48121 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48181 gttttcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48241 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48301 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48361 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48421 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48481 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48541 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48601 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48661 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48721 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48781 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48841 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48901 ttgaaagaaa agtgtcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48961 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    49021 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49081 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49141 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49201 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49261 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49321 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49381 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49441 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49501 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49561 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49621 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49681 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49741 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49801 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49861 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49921 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49981 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    50041 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50101 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50161 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50221 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50281 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50341 ttcagatagt caagatcctg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50401 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50461 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50521 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50581 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50641 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50701 cttcgccctt cgcacgatgc agttggcgca aatgtacggc tatgtcattc cagagacact
    50761 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50821 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50881 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50941 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    51001 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51061 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51121 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51181 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51241 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51301 aagacaggtg caagaatcga gaaaaagacg aatggcaagt aatgcaacgc gctaactttc
    51361 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51421 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51481 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51541 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51601 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51661 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51721 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51781 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51841 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51901 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51961 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    52021 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52081 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52141 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52201 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52261 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52321 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52381 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52441 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52501 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52561 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52621 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52681 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tatttttttt atttttcttc
    52741 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52801 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52861 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52921 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52981 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    53041 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53101 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53161 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53221 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53281 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53341 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53401 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53461 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53521 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53581 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53641 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53701 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53761 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53821 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53881 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53941 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    54001 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54061 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54121 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54181 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54241 gggattcttt gagtctattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54301 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54361 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54421 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54481 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54541 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54601 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54661 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54721 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54781 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54841 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54901 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54961 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    55021 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55081 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55141 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55201 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55261 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55321 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55381 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55441 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55501 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55561 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55621 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55681 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55741 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55801 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55861 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55921 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55981 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    56041 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56101 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56161 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56221 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56281 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56341 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56401 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56461 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56521 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56581 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56641 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56701 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56761 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56821 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56881 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56941 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    57001 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57061 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57121 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57181 cttctacttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57241 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57301 cgatagattg cagaatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57361 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57421 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57481 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57541 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57601 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57661 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57721 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57781 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57841 tttatgaaag caggaacctg ctagcatgtg taattaatta tttcttcagg ataggatagg
    57901 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57961 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    58021 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58081 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58141 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58201 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58261 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58321 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58381 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58441 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58501 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58561 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58621 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58681 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58741 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58801 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58861 tgattttgtt ttagttaatt taaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58921 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58981 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    59041 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59101 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59161 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59221 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59281 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59341 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59401 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59461 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59521 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59581 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59641 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59701 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59761 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59821 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59881 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59941 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    60001 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60061 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60121 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60181 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60241 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60301 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60361 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60421 tctgaggttt attataacta tctattgaga aagtatcaag ctagcaattc cttgtttatg
    60481 aattcattga cagaaattta taaaaagaat attatgtact aaaataataa aaaaag
//
LOCUS       DAZULO010000008        51215 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000008 DAZULO010000000
VERSION     DAZULO010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.008"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            330..1175
                     /locus_tag="ACG8OA_000513"
     CDS             330..1175
                     /locus_tag="ACG8OA_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4975357.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="ACG8OA_000514"
     CDS             1456..2676
                     /locus_tag="ACG8OA_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS4975358.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="ACG8OA_000515"
     CDS             2673..5393
                     /locus_tag="ACG8OA_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4975359.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="ACG8OA_000516"
     CDS             5377..6321
                     /locus_tag="ACG8OA_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4975360.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="ACG8OA_000517"
     CDS             complement(6390..7400)
                     /locus_tag="ACG8OA_000517"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4975361.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="ACG8OA_000518"
     CDS             complement(7580..7924)
                     /locus_tag="ACG8OA_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS4975362.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="ACG8OA_000519"
     CDS             complement(7917..8351)
                     /locus_tag="ACG8OA_000519"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFS4975363.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="ACG8OA_000520"
     CDS             8489..9226
                     /locus_tag="ACG8OA_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4975364.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="ACG8OA_000521"
     CDS             9219..10430
                     /locus_tag="ACG8OA_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4975365.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10634..11416
                     /locus_tag="ACG8OA_000522"
     CDS             10634..11416
                     /locus_tag="ACG8OA_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFS4975366.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="ACG8OA_000523"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="ACG8OA_000523"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFS4975367.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="ACG8OA_000524"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="ACG8OA_000524"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFS4975368.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="comGA"
                     /locus_tag="ACG8OA_000525"
     CDS             14132..15061
                     /gene="comGA"
                     /locus_tag="ACG8OA_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFS4975369.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /gene="comGB"
                     /locus_tag="ACG8OA_000526"
     CDS             15018..16067
                     /gene="comGB"
                     /locus_tag="ACG8OA_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFS4975370.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /gene="comGC"
                     /locus_tag="ACG8OA_000527"
     CDS             16064..16342
                     /gene="comGC"
                     /locus_tag="ACG8OA_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFS4975371.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /gene="comGD"
                     /locus_tag="ACG8OA_000528"
     CDS             16317..16787
                     /gene="comGD"
                     /locus_tag="ACG8OA_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFS4975372.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16759..17106
                     /locus_tag="ACG8OA_000529"
     CDS             16759..17106
                     /locus_tag="ACG8OA_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975373.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            17069..17494
                     /gene="comGF"
                     /locus_tag="ACG8OA_000530"
     CDS             17069..17494
                     /gene="comGF"
                     /locus_tag="ACG8OA_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFS4975374.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /gene="comGG"
                     /locus_tag="ACG8OA_000531"
     CDS             17466..17825
                     /gene="comGG"
                     /locus_tag="ACG8OA_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFS4975375.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="ACG8OA_000532"
     CDS             17997..19004
                     /locus_tag="ACG8OA_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4975376.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="ACG8OA_000533"
     CDS             19029..20213
                     /locus_tag="ACG8OA_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFS4975377.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /gene="abc-f"
                     /locus_tag="ACG8OA_000534"
     CDS             complement(20650..22233)
                     /gene="abc-f"
                     /locus_tag="ACG8OA_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFS4975378.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22338..22466)
                     /locus_tag="ACG8OA_000535"
     CDS             complement(22338..22466)
                     /locus_tag="ACG8OA_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975379.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(22811..22954)
                     /locus_tag="ACG8OA_000536"
     CDS             complement(22811..22954)
                     /locus_tag="ACG8OA_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFS4975380.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23176..23655)
                     /locus_tag="ACG8OA_000537"
     CDS             complement(23176..23655)
                     /locus_tag="ACG8OA_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4975381.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23728..24012)
                     /locus_tag="ACG8OA_000538"
     CDS             complement(23728..24012)
                     /locus_tag="ACG8OA_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975382.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24090..24284)
                     /gene="ssrS"
                     /locus_tag="ACG8OA_000539"
     ncRNA           complement(24090..24284)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8OA_000539"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24356..25642)
                     /locus_tag="ACG8OA_000540"
     CDS             complement(24356..25642)
                     /locus_tag="ACG8OA_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFS4975383.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25780..26451
                     /locus_tag="ACG8OA_000541"
     CDS             25780..26451
                     /locus_tag="ACG8OA_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFS4975384.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26417..26905
                     /locus_tag="ACG8OA_000542"
     CDS             26417..26905
                     /locus_tag="ACG8OA_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFS4975385.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26918..27865
                     /gene="prmA"
                     /locus_tag="ACG8OA_000543"
     CDS             26918..27865
                     /gene="prmA"
                     /locus_tag="ACG8OA_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFS4975386.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27866..28630
                     /locus_tag="ACG8OA_000544"
     CDS             27866..28630
                     /locus_tag="ACG8OA_000544"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFS4975387.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28863..31076
                     /locus_tag="ACG8OA_000545"
     CDS             28863..31076
                     /locus_tag="ACG8OA_000545"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFS4975388.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31087..31533
                     /gene="dtd"
                     /locus_tag="ACG8OA_000546"
     CDS             31087..31533
                     /gene="dtd"
                     /locus_tag="ACG8OA_000546"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFS4975389.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31704..32963
                     /locus_tag="ACG8OA_000547"
     CDS             31704..32963
                     /locus_tag="ACG8OA_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFS4975390.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33052..33999)
                     /locus_tag="ACG8OA_000548"
     CDS             complement(33052..33999)
                     /locus_tag="ACG8OA_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4975391.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34393..35685
                     /gene="hisS"
                     /locus_tag="ACG8OA_000549"
     CDS             34393..35685
                     /gene="hisS"
                     /locus_tag="ACG8OA_000549"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFS4975392.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35700..37469
                     /gene="aspS"
                     /locus_tag="ACG8OA_000550"
     CDS             35700..37469
                     /gene="aspS"
                     /locus_tag="ACG8OA_000550"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFS4975393.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37578..38453
                     /locus_tag="ACG8OA_000551"
     CDS             37578..38453
                     /locus_tag="ACG8OA_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4975394.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38719..40602
                     /locus_tag="ACG8OA_000552"
     CDS             38719..40602
                     /locus_tag="ACG8OA_000552"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS4975395.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40677..40961)
                     /locus_tag="ACG8OA_000553"
     CDS             complement(40677..40961)
                     /locus_tag="ACG8OA_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS4975396.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40951..41685)
                     /locus_tag="ACG8OA_000554"
     CDS             complement(40951..41685)
                     /locus_tag="ACG8OA_000554"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFS4975397.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41814..42437
                     /locus_tag="ACG8OA_000555"
     CDS             41814..42437
                     /locus_tag="ACG8OA_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFS4975398.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42629..43750
                     /locus_tag="ACG8OA_000556"
     CDS             42629..43750
                     /locus_tag="ACG8OA_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS4975399.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43757..46885
                     /locus_tag="ACG8OA_000557"
     CDS             43757..46885
                     /locus_tag="ACG8OA_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HFS4975400.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46904..50098
                     /locus_tag="ACG8OA_000558"
     CDS             46904..50098
                     /locus_tag="ACG8OA_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFS4975401.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50244..50951)
                     /locus_tag="ACG8OA_000559"
     CDS             complement(50244..50951)
                     /locus_tag="ACG8OA_000559"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4975402.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16763 a         8942 c        10637 g        14873 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc ttgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatctcaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggctt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cgggacagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg aaactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acagtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatat cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccctggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttat
    23041 tttacttgaa ctcattgact gttttccctt tattttaaac attataaaga ccgatgagtg
    23101 cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa aaaaacctgc cgatagttgg
    23161 caggtcgtct acgtgttaag ctttggtgtg gtctttagct ggcaattggt tttctaaatc
    23221 agttcggaca acaagcacat cgcaagctgc gtttctaata acatattcag aaacagaacc
    23281 aataaataaa cgttctacag cattcaatcc tgtggctcct aacataatca aatctacttg
    23341 attatcttct gggatttctc tggcaataat ttgttttggt gagccatatt ctacgacaga
    23401 ggttacatta ttcaagccag ctttcttcgc attgctttca taatctgcca atgtttgctt
    23461 tgccatttca gtagcttgtt ctgcaagcat tccatcaaag gatgaaacgg tttggaaaga
    23521 acgtgtgtca atgacatgtg ctaatagaag ttctccgtta tttcggatag ctacattgac
    23581 cgccttttta aaagctaatt cagcttcatc agaaccatct acggcaacca tgattttttt
    23641 gtattgttgt aacataagca ttcactcctc aagtcaatcg tattgtactc ttttattgta
    23701 gaacacttta gggaaaaaga gaattattta ctgctttggt tcagcaattt tttttgcaaa
    23761 tcggatactg aaggtggcag aaacaataat tacgatcagt aaaaagacca aggcaataaa
    23821 gcgttgattg aaaaaagctg ttaaaatacc cattacgcct aaaacagcat atgcagcacc
    23881 gtaaaattgt aggaatcctt gatttttatc atttttgttg atcaatacaa aaaagatatc
    23941 ctgtttatta aataaataat agctaacgaa taataatact aaagtggcga taatcattaa
    24001 aatcctaatc atgaacaaat cctccaaaat aagcaacaaa tagaaaaagc aatttgccaa
    24061 gagcaaattg ctttattctt taaaagatgg taatctccgt taatgccgtt gtttgtcctg
    24121 tagtattgat cccgcgaatt caagcaggtg ggtcccatgg tcgtagcttc gaactaccaa
    24181 atgataaggc atgttaccag cagtgacaca agacgggtcc caagatatca ataaaaatgt
    24241 tcggtcaaca cacaaagagg acaattcgaa catcacagat ttcccacctc tatagtagca
    24301 tatgaattta aaaagagcaa tgagagagct cgcagaaaca gaaccgtttt cttgtctagt
    24361 tttttggagg atgctttttc cactctttta tacggtaata ctgttgaccc agagcctgtt
    24421 catatttccc tgtatctttt ggctgataat actgagcatt cttcagttta tctggtaaat
    24481 attgctgatt gacccaagcc tgatcaaaat gatgaggata ctgataaccc acgccacggt
    24541 tgagtgattt cgcacctttg taatggctat ctctcaaatg atcaggaaca tctccagctt
    24601 tgccctctcg aatatcagca agtgctgcat ctaatgccat gtaggcagaa ttggattttg
    24661 gcgacaagca aagatcgacg acgacgtctg ctagtgggat acgtgcctca ggcaatccca
    24721 atttttcagc agctaataca gcgttcactg tacgagccgc agcagcagga ttacctaaac
    24781 ctatatcttc ataaccgata accatcagac gccggcaaat acttgccaga tcacctgcct
    24841 caactaaacg ggcgagataa tgaagtgcag catccacatc acttccacga atcgattttt
    24901 gaaaagccga aataacgtca taatgagcat ctccattttt gtcatgggta agagcttttc
    24961 tttggacaca ttcttcaatg atcgacagtg taagatgaat ccttccttct ttatctgaag
    25021 gagtcgatag tgtagccagt tccagcccat tcaatgcact ccgcaaatcg ccgttcgttg
    25081 cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat tgcttgttgt ccaaggccgc
    25141 gttctttatc ccttaaagca tgttcgactg ccaactggat gtcttgttct gttaatggtt
    25201 tgacttcaaa gatctgtgta cggctgcgga tcgctggatt gatggtaata taaggattct
    25261 cagtagtcgc tccgatcaag atgattcggc cactttctaa atgaggaagc agaaaatcct
    25321 gttttgtttt atctaatcgg tgtacttcat ccaataagag gatcactgta ccactcattt
    25381 ttgcttcttc cgctacaact tgtagatcct tttttgaatc tgtagcggcg tttagcatgc
    25441 gaaatgcata attagtagag ccggcgatcg cacttgcgat actcgttttt ccagtaccag
    25501 gaggtccata tagaatcatg gaagagagca tgcgagcatc gaccatgcgg cgtatgattt
    25561 ttccttcccc gactagatgc tgctgaccaa cgacttcatc gatcgttcga ggtctcatgc
    25621 gataagccaa tggctgttgc ataggatttt cctccttctt gtttttacgt cccttaatta
    25681 tatcatattt agcaagatgc ctagaaagaa cagccttctg ttgttttgtt ttcatagctt
    25741 atccaactaa tattgtgtat gataaagtag gtgagaaaaa tgacggataa acaaaagata
    25801 gatgaatttt tttggaataa aacgacgcca gaagtggcac gggatttatt ggggatgtat
    25861 ttggagcatg acacaccaga aggaagatta gctggatata ttgtagacgc ggaagcatac
    25921 ctaggtccag aagacgaggc cgcccatagc tatggtttaa gaagaacacc gcgtgtgcgg
    25981 gcaatgtatg aaaaaccagg tacgatctac ctttacacga tgcatactca tcggattttg
    26041 aacatcatta ctcagccaga agggattcct caaggagtaa tgatacgtgc aatcgaaccc
    26101 gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa aaactggtcc agatatcagc
    26161 aacggtccgg gaaaattagt ggaagcatta gcaattcctc aagaacttta tggtcagtct
    26221 atagcagata gttcgttacg cttagttttt gagaaaaaaa agacaccaaa aaagatcaat
    26281 gctttgccta gaatcggtat cccaaacaaa ggtgtatgga cagaaaagcc gttgcgtttt
    26341 gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa accaaatcga gaaagattgg
    26401 ggttggagga aagaaaatga aaaagaagga tctattaaca tatttagatg aaataattga
    26461 aaaacaagtg acagactatg atgtagcttt agattgggat acaaaaaatc atacgatcga
    26521 gatcgttgtt cgcttgtttg cagaaaataa agcacaattt gagattgatg atgcagaagg
    26581 gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc gtacttcttt ttaatcctca
    26641 gaaatctgtt tttgatgaac gcaattatct agcagtaatc ccttatgaag gaaaaaaagg
    26701 gttggcaaaa tcagtagctg attctttagt ggaatatcta aatgaagtat tagctcaagg
    26761 gcaaagcgat ttgcttgatt ttttggatga agacgatgac gaagctgtat tcgagcttca
    26821 ctgggataat caacaactag cagaattagt agaagtaaaa cagcaaaatg aagcagatac
    26881 ttatttaccg tacccaagtt attaaggagt gacagatatg aaatggaatg aagtaaaagt
    26941 agaaactgaa agtgaagcag tcgaagcagt tgctaatatt ttgatggagg caggggcgag
    27001 tggtgtcgcg attgaagatt ctttagatgt tgaacatttt cagtcggatc aatatggtga
    27061 gattttagat aaaaatgatt ttacatcgct gaaagaaggc gcactagtca tggcttattt
    27121 tccggaaacg actttcttac cggaaatctt gccttttatc aaagatcgta tcacacaatt
    27181 acctgagttt ggtctagcta ttggaaaaaa taaagtaaca gttagtgaag tagaggaaag
    27241 caactgggca acagcctgga agaaatacta tcatccggtg cgtattacgc gaatgctgac
    27301 ggttgcgcca agctgggaaa actaccagac agaagatcct ctagaaaaaa ttatcacact
    27361 agatcctggt atggcatttg gcacaggcac tcatcccacg acttccttga cattgacagc
    27421 actagaaaca gtcttgcgtg gtggtgaaac attgctagat gtgggaactg ggtcgggcgt
    27481 gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc tatgcctttg atttagacga
    27541 agtagccgtt cgttcggcga aagaaaatat ggatatgaat gaaatagcca aagatgtcca
    27601 tgtatcagct aacgatcttt tgacaggaat cgagattgaa agtgatgtaa tcgttgcgaa
    27661 cattttagca gatatcatct tattgatgat cccagatgct tggcgtttac tgaagcaaac
    27721 aggcacgctg atcgtttcag gaatcattga agagaagaag caaatggttc tggatgccat
    27781 gttagaacaa gggtttatcg tggatcaaat tttccagcaa aaagactggt atgcaattat
    27841 tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt ttaaaagaat catatgaatc
    27901 aaaagactat tttgttttag aaggggaaaa ctatcaccac attgtacggg ttatgaggat
    27961 ggaacccgag gatgaagttt ttttggcatt caatgatcaa atagccattg ttgcagaaat
    28021 cacggagatt actgaagagg ctgtttatct gaaagaaatt aaaaaagaag aaaatcaaaa
    28081 ggaactgcca agttcgatta ccatagcgag tggctatcct aaaggggaaa agctggattg
    28141 gatcgttcag aaatccactg aattaggagc gcaagctttt cttggtttcc cggcatcagc
    28201 ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga caacaacggc ttgaaaagat
    28261 tgctcaagag gcagcggaac aatcccatcg gcagatcgtg ccatcagtag agttattgga
    28321 aacgaaacaa gatttatttg agcggctaga tgattatgac gtggtcttgg ttgcttatga
    28381 ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa attcttagtg aaacaaaacc
    28441 agatagtcga atcttagctg tctttggacc agaaggtgga ttatcccgcg atgaagtcga
    28501 ttattttgta gaacacaaag ggatactttg tggattagga ccaagaatcc ttcgtacaga
    28561 aacagcacct ctttatttat taagtgctgc tagctatcaa tgggaattaa tgaatccttc
    28621 tttcagctaa cgttcttcat taataaaaga gtatcgacag aaagaattga aaaaatagtc
    28681 aacaatttgt ataatagaaa caattattta tacaatggat acgaaaaatc gtttgaaaaa
    28741 gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac tgcacggatt ttgggacaat
    28801 tatcaaacag atatttgtcc cgattcccgt tttaccgaaa aaagaaaggg ggatcgattc
    28861 ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat aaagctcgtt agtcaataca
    28921 tgggtcccga acatgtagca tttgttgaga aagcatgcga atacgcgact gctgcacatg
    28981 acggacagtt tagaaaatca ggcgaaccct acattattca tcctatccaa gtcgcaggta
    29041 tattagcaga tttaaaaatg gatccccata cagtggctac aggcttctta catgatgttg
    29101 ttgaagatac agaaatcact ttagaagatc tgagagaaga atttggcgat gacgttgcta
    29161 tgttagtgga cggcgtaacc aaattaggga aaataaaata taaatcccac gaagagcagc
    29221 tagcagaaaa ccaccgaaag atgctgctag caatggctca agatttacga gttatcatgg
    29281 tcaaattagc tgatagacta cataacatgc gtacgttgaa gcacctgcga gaagataagc
    29341 aaagaaggat cgctcaggaa actttagaaa tctatgcgcc tcttgcacat cgtctaggga
    29401 tcagccggat caaatgggaa ttagaagata cagcacttcg ttatctaaat ccaaaacagt
    29461 attaccgtat cgtccacttg atgcagacga agagagaaga acgtgaaaaa tacgtaagtg
    29521 gtactgttga agatattcga atagcgacag aagagctggg gatttttgca gaaatctatg
    29581 gacggccaaa acacatttat tcaatctatc gtaaaatgaa agatcagaaa aagcagttca
    29641 acgaaattta tgacttgcta gctattcgag tgatcgtaga ttcaatcaag gattgttatg
    29701 ctgtattagg agcaatccac acaaaatgga aaccaatgcc cggcagattt aaggattata
    29761 ttgccatgcc aaaagctaat atgtaccaat ctttgcatac taccgtgatc ggtccggcag
    29821 gaaatccggt agaaattcaa atccgaacac aggaaatgca tgaaatcgct gaattcgggg
    29881 ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa agtagaacca gatggtatga
    29941 cgaaacaatt aagctggttc catgagatac tcgaacttca agacgaaagc tatgatgctt
    30001 ctgaatttat ggaaggcgta aaaggagata tctttagtga taaagtctac gtcttcacac
    30061 caaaaggaga cgttactgag ttaccaaaag gatccggacc attggacttt gcatacagta
    30121 tccatacaga tatcggtaac aaaaccactg gtgcaaaagt aaatggcaaa atggtgcagc
    30181 ttgattacaa attgaaaaac ggagatatca ttgagatcat gacttctcca aattcatttg
    30241 gcccaagtcg cgactggttg aaattagttg ctactagcaa agcaagaaat aagatcaaac
    30301 gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa aggccacgaa tccgtggtca
    30361 aatgtattac agatctagga tttacgccta aagatatttt gacgaagaac aaactgcaag
    30421 aagcactcga tcgttttaat tatcaaacag aagatgatct ctatgcagct gtagggtatg
    30481 gagaagttag ccccttgacg atggccaatc gtctgactga aaaagaacgt aaagaacaaa
    30541 aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa tcagccgaaa aaagaacctg
    30601 acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca aggggtagaa aacttactaa
    30661 ttcgtatcag tcgctgctgt aatccgattc ctggtgatga tatcgttggt tatatcacta
    30721 aaggcagagg gatatccatt catcgtcgag attgtccgaa tgttcagcct gacaaaccaa
    30781 atgtagcaga acgtttgatt gaagtcgaat gggaagatac atcgaataca cgaaaagagt
    30841 atgatgcaga tttggaaatt tacggctata atcgttcagg cttattgaat gatgtacttc
    30901 aaacagtcaa tgcgctaacg aaaaatctca acagcgttga agcacggacg aataaagata
    30961 aaatggcgac gatccatttg acggttggta tccagaattt atcccaccta aagagtatcg
    31021 tggataaaat caaagcagta cctgatgtct acagtgtacg ccggacgaat ggataggagg
    31081 acagatatga gagcagttgt tcaacgggta agtaaagctg ctgtgtcagt cgatcagcaa
    31141 gaaattggaa aaattggcta cggcctactt gttttattag gcattcatga aaccgatacg
    31201 caaaaagatg tggactatct ggtaaaaaaa attgcacaaa tgcgtatttt tgaagatgaa
    31261 caaggaaaaa tgaatattag tgtagaagat gtgggaggac aaattttaag tatctctcaa
    31321 ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa gctttgtgga agccgcacga
    31381 ccagaaacag ctattccttt atacgaatca ttcaatgaag gaatcagaag tcgcggcatc
    31441 acagtcgcta ccggacaatt cggagcagat atgtcggttt ctctcgtcaa tgatggacct
    31501 gtaacgatca ttattgattc taaaaacaaa taaagtaatc tggttttcac tagtaaaaac
    31561 tatgaaacac gtcgcttaag ttagactgat aaactctaac ttaagtggct tttttgtctg
    31621 caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta agcttaaatt atacaagtta
    31681 ttttttatag gaggttgctg aagatgaaag aagtaccagt agtagatatc aaaatcacag
    31741 atttaaaaaa attgtatcaa aaaggataca acatgttgga ggagttacgc catgaagcgg
    31801 acgctcccgt cgtcaaagca aagattttca ataaagaagc tatcacgatc tacggatcat
    31861 cagcagcaaa agtattttat gatccaagga atttcaaacg aaaaggtgct atgccaaaat
    31921 tagtattgaa gacacttttt ggtcaaggtg gagtacagac tttggatgga gctgctcatc
    31981 accatcggaa aaatattttt atggatctaa tgacaccgga aagaatggaa gattatcatc
    32041 gtatcttaga taagaatctg acacaagcac ttgaagcaca gcatggtcaa tttgaactat
    32101 ttgatttaag taagatggtt ttctttacat ctatttgcga gtgggcaggt atcaatttat
    32161 cagcgatttc taaagatgag gtcgaaaaat tagcagaata tcaaatctct atgatcagcg
    32221 gaacgtttac ttctccgatt gatcacataa aaggtgtcga gaatcgcaaa aaatcagaaa
    32281 aatgggcgca aggcttgatt gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg
    32341 tggcattata tgcttttgcc aatgccacag atttagatgg tcagctttta cctctggaag
    32401 tggcagctgt tgaattattg aacatcatcc gtccaacggt tgctctaact gtgtgggcgg
    32461 cattgatggg acatgcactt ttcagtagac cagatttgta tcagcaatta aaaaatgact
    32521 tttcgacgtt acaagatccg tttattcaag aaatgagacg ctattatcct ttcttcccaa
    32581 tgctacctgc gatttcgctg aaagaagtag aagtagatgg ttatcgtata ccagaaggca
    32641 gttgggttat tttagattta tatggaacag accatgatga acgaacagtt gaagcgccag
    32701 attcctttat gatcaaacgg tatgtaggga aagcaaaaga tatttcctat aaagaagaat
    32761 atgagatgat tgcacaaggc gggggaaatt ttcgacaaat gcatcgctgt gcaggagaat
    32821 ggatcactct tcatagtcta cgtgtgtttt ccgatcagct agtaaataag tttgagttct
    32881 ctgtacctga acaagattgg acgattccat tcaatcagtt tccgacttat ccgaatagcc
    32941 gagcgctact ttacaagaat tagattagca taaacttatc gaaaaaaagg aagatcaaac
    33001 agtgctgatc ttcctttttt catattacta taaattgata aggtcaattg atcaaaatcc
    33061 acgaaatgct tcatcaatgg tttgataatc ttcattggat aaatgcaaat ctaaagcttg
    33121 gacattgtca gatacttgct ctggacgctt agctccaggt ataactacag acactcgtgg
    33181 attcttcatg taccaagcta aaacaagttg cgcaggagtg gcttggtatc ttttagcaat
    33241 ggggcgaagt acatcgacag cggttaaaat ttctctgaaa cgttctcctt ggaaatctgg
    33301 atttcttttt cgaggatctc cttcaccaaa ttgtttttcc tcaccaagtt catattttcc
    33361 tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa ctgattttgt ttttttcaag
    33421 ataagggaat aattcttttt cagcctgccg atggatcaaa ctgtatttat cttctactac
    33481 atcgacatat ccatctgcat tcgcttcttt caattgttct aacgtgaagt ttgacacacc
    33541 gactgctcgg atttttcctg cttcttttaa ttcgtgcaat gtagcaactg attcattttt
    33601 tggtgtgctt tcatccggaa agtggatata aaaaatgtcg atgtaatcag tttgcagacg
    33661 ttttagtgca tcttccaccg attgtttgag aaactcagga gaattatcaa atgttcgccc
    33721 tttatttggt acatgagcag cttttgtagc gatgacgaca cgtgagcgat catattcttt
    33781 ttccttcagt acctcgccaa tcagttcttc ggaacggccg tttccgtaag cataagcggt
    33841 atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact acttgttttc ctgtctcatc
    33901 ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt gctcctaatc caagctgttc
    33961 tgcgtagact tgtgaatgtc cgattcttat ttctgccata gctgattcct cctttttgtc
    34021 tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt tttgtgaggg gaaatagtca
    34081 gatatctctt gactttgaga ctggttttta gtaggttata agtaatatcg aatgtcaatg
    34141 aaagagcgag taagtttttg aaactttaca agagaggatc atcgaggctg aaaatgatct
    34201 aaagtttcat tagcctaaag acactctgga gatggacgag tgaaaagccg tcccgcgtac
    34261 gagcgttatt cgtaagagga aagagaaatc tttcaaaatt aggtggtacc gtgtatgaat
    34321 ttcattcacc cttgttcatg atgaacaagg gtgatttttt tatttacata gctaagaaag
    34381 ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa atgatatttt gccaggcgaa
    34441 tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat tattcagaga ttatcagtat
    34501 aatgaaatgc gtacaccgat ttttgaacac tatgaagtga tatcccgaag tgtaggggat
    34561 acaacggata tcgtttcaaa agagatgtat gatttttatg acaaagggga acgccatgtg
    34621 acgcttcgac cagaaggtac agcaccaatc gttcgttctt ttgttgagca taaattgttc
    34681 ggcccagagt atgcgaaacc gttcaaaact ttttatatgg gaccaatgtt tcgctacgaa
    34741 cgccctcaaa aaggacggtt acgtcaattc catcaaatcg gagtagaagc atttggatca
    34801 gaaaaccctg caactgatgt ggagagcatg gtgatggcac ttgatttctt caaacagttg
    34861 gggatcacgc aaatccggct agtcatcaat tcattaggtg ataaagaaac gcgtcaagcc
    34921 tatcgtcaag cattgatcga ctatctttta ccttttgaat cagagctgag tgaagactct
    34981 aaacgccgcc ttcatgaaaa tccattacgc gtattggaca gtaaagacaa acgagatcaa
    35041 aaatttgtgg cagaagctcc atctatctta gattatctaa gcgagcctgc tcaaaaacat
    35101 tttgataccg ttgtttccat gctggatgca ttggctgttc cttatgaaat cgacagtaat
    35161 atggttcgcg gactagatta ttacacacat acgatttttg aaatcatgag tgacgcacca
    35221 aaaatggggg cacaagcaac gatttgcgca ggcggacgtt atgacaatct agtggaagaa
    35281 ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg gtatcgagcg attactcttg
    35341 actatggagg cagaggaagt agttatccct gcatttaatg agttggatgc ttacgtggtc
    35401 gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg tccaagcgat tcgaaacttt
    35461 ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa aagcgcaatt taagacagca
    35521 gataaagcga atgccaaatt agtattggtg atcggtgaag atgagcttgc tagtcaaacg
    35581 attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt ttccattaga agagatatat
    35641 gaacattttg atgaagtcta tgatgaaatg actttattcg agtaggagga aaaaaataaa
    35701 tggcacaaag aacgatctat tgcggattag tttccgaaca acaattagaa caaacagtta
    35761 cattaaaagg atgggtacaa aaaagacgag accttggggg agtaatcttt atcgacctac
    35821 gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga aatcaacaaa gaagcatggg
    35881 aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt gactggggaa gttagaagac
    35941 gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga attcgaagtg attgcaagtg
    36001 aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca aattgaagat gatcaagcaa
    36061 tcagtgacga aatacgtttg aaatatcgtt atttggatct gcgtcgtcca aaaatgacta
    36121 aaaacatcaa attaagaaat gcgacaacaa aagcgattcg tcattattta gatgataatg
    36181 actttctcga tatcgaaaca ccttacctag ggaaatctac accagaaggc gcaagagact
    36241 acctagttcc ttcacgtgtc catgctggac atttctatgc gttgccgcaa tctcctcaat
    36301 tgtttaaaca attgctgatg aatgctggat tcgaccgtta ttatcaaatc gttcgttgtt
    36361 tccgcgatga agatttacgt ggagaccgtc agcctgaatt tacgcaagta gatattgaaa
    36421 caacgttctt aacagcagaa gaaatccaaa cttacacaga gggattgatt gcgaaggtaa
    36481 tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc tcgtatgacc tatgatgaag
    36541 caatggctcg ctacggcagc gacaaaccgg atacacgttt tgatatggaa ttgatcgatc
    36601 taagcgatac agtcaaagaa gtagaattca aagtcttcca aatggctctt gaaaacggcg
    36661 gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg ctactcacga aaagacatgg
    36721 atcagttagg tcaatatgta ggacaattcg gagctaaggg gcttgcatgg ctgaaagtag
    36781 aagaagacgg attgaaaggt ccaattgcta aattcatggg agaagcaaca gaagcaatca
    36841 ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt tggcgcagat aaaagtgaaa
    36901 tcgtcgctgc tgcattaggt gctatccgga cacgtttagg aaaagaatta ggactaattg
    36961 atgaatcgaa attcaatttc ttatgggtaa ctgattggcc acaatttgaa ttttcagaag
    37021 aagaaggtcg atatgtctct gctcaccatc cattcacgat gccaaaagaa gaagatatcc
    37081 cgttattggc tgaagaccct gcaaaagtct atgcagaagc ttatgatatc gtcttgaacg
    37141 gttatgaatt aggcggaggt tctatccgta ttcatactcg tgagcttcaa gaaaaagtac
    37201 tagaaactct aggctttaca aaagaacaaa tggaagaaca atttggtttc ttgttaacag
    37261 ctttagatta cggattccct ccacatggcg gtattgcatt aggattggac cgttttgcaa
    37321 tgttattagc cggagaagat aatattcgtg aagtcattgc ctttcctaaa aacggaaaag
    37381 cagcagatgt gatgagtgat gcaccaagta tggtttcgcc tttacaattg ttcgaattga
    37441 atattgatgt aacgaatata gaagaatagc cgataaaaag gtaaaaagga tttgtcgaat
    37501 acgcttcttt tgacaacctt gatttaattg aaaaacactt tataatgaag ccataacata
    37561 taaagcgggg cttttccatg aaaaaaacat tcgaaaattg ggcttatcat gattacacga
    37621 ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc agtcgcattg aatttctttt
    37681 atcagccagg acatatttat tcaagtggta ttactggtct agctcagatt ttgacgactt
    37741 tgtcaaaatc actgatagga ttcgaagtac cggtatcaac aactttgtat ttactgaata
    37801 tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa gtttacaatt ttcactttta
    37861 tcacagtcac tttgacctcc ctttgtatcc atattttacc ggaaacagtc ctatctaccg
    37921 atccaatcat ctgtgcgttg tttggggggg ctgtgatggg actaggtatt gggctagcgt
    37981 tgagaaacgg cctatcatcg ggcggtctag atatcgtgag tatcacgatt cgtaaaaaaa
    38041 caggaaagtc gattggttct atttcgatct acttcaatgc attgatcgtt tttgttgccg
    38101 gaattctatt tggctggcaa tatatgtttt acagtacgtt gtctattttc gtcagtggaa
    38161 aagtaactga tgtcgtttat actaaacaaa agaagatgca ggtaatgatc gtaacgaaac
    38221 atccagaaaa agtagtaaat gaaatccaaa actgtatgcg tagagggatc acgatcatca
    38281 atgaagcaga aggtgcttat aagcatgata agcagacagt attgatgacg atcgttacac
    38341 gatacgaact gcctctactt agagaagcaa tgaaagcttc tgatccacaa gcttttgtaa
    38401 gtattgcaga aaacgtccag atacttggtc gtttctatga ggatggcatt tagatcgagt
    38461 ttgacaaaaa ttcatgaaat actgctgtag acagacgttc gtttgtctgc agtttttttc
    38521 taccatagta gaatcagcaa agaaaaactg cttttttatt tgaaattttc ttaaagttga
    38581 aaaaaagcat gtttattttt ttaaatttat gggatgatag aaaaaaatag tagagaaagg
    38641 acacgattct acttgctatt ttcacttaat acccgtatat tacgaatgaa aaaagtttat
    38701 gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa aaaacagccg ctacatcact
    38761 gggtttgatg ggctccgttc attagctgtg gtgggtgtta tcttctatca ccttctgcca
    38821 actagtatga aaggcggcta tttgggcgtt ccgatttttt tcgtcgtatc tggttatttg
    38881 atcacggatc tgctgcggca ggaatgggaa caaaatggaa aaatcaatat ttggcaattt
    38941 tatgtcagaa gaatgaagag actctatccg gcacttgttt ttatgcttgt gacagcttcg
    39001 gcttatatca ctttgtttca gcgagggctg ttaaacaatc ttcgaggagt tgtggtcagc
    39061 agcttactgt atgtcaacaa ctggtggcaa atcaataacg gtttatctta tttcgatcga
    39121 tttgctaatg agtcaccgtt tacgcatatc tggtctttag cagtagaagg tcaaaactat
    39181 ttggtttggc cgatcttgtt tgtattgttg atggtatttg ttcgtaaaag aaaatggatc
    39241 ttttatactg tgttaggtac atccttcctc tccgccatct tgatgatgat cttgtataca
    39301 ccaggaggcg atccaacccg tgtttattac ggtaccgaca ctcgtctgtt ttctatatgg
    39361 ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt taaagaaaaa catcccgaaa
    39421 caagcgaaaa atattctgaa tatcgctgga ggaatctcgc ttttggcact gattctttgc
    39481 ttcttctttt tagatgatca ttacaatttc atctattacg gaggaatgct cctagtgagt
    39541 ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg gagcaagttt gaatcgatgg
    39601 ctgacaaatc cagtatttac ttggattggc aaacgaagct atggcattta tctgtatcag
    39661 tttccagtta tgatttttta tgaagcaaag gtgactgata ttgccgatca tgttttgttg
    39721 catactttag tggaaatcac attgattcta atcatcagtg aactttctta tcgatatgtg
    39781 gagcgtccgc tagcacgttt tgattatggt cgtacattcc aagtgatgaa aggatggttc
    39841 agcaaaccga tcatcagtaa gagcaaacca tgggttgttc caggcacatt acttgtagca
    39901 attgcactcg ttggtttcgt tacggcaccg aaaaacgctg taacagccga tcaaaaacaa
    39961 ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag aaaccagaaa caattcagaa
    40021 aacaccacta gttcggttga ccaagcgatc cttgataagt acgagttgtc caaggcacaa
    40081 gggaataagg cacaaaaatt agaactaacg gcatttggtg attcagttat gttggatgct
    40141 gcaacggatc tacaagaact ttatccaaaa gtagtggtag atggagatgt cggacgacaa
    40201 ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac aagatttgtt gaaagacact
    40261 gttctgattg gactaggaac gaatggctca ttctcggaag ctcagtttga tagtgtgatg
    40321 gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc gagtaccgac gaagcgctgg
    40381 caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa aatacgacaa tatgacgctg
    40441 attgattggt acagctacag taatgaccat agtgactggt tttatgatga tcaagtccat
    40501 ccaaatcctg aagggatgac acattacatt cacttagtca gcgaagcctt gctaggagga
    40561 agttcaagta cgaacagcag tgagacaaac gtgacagaat gatagctaca aaaaacttca
    40621 ggcaaaaaca tttgtttttg cctgaagttt ttttatattt accgtgtttt taacattcat
    40681 atagattcag gaagcaaaac ggttgagtgt gtctgtaaat aacgttcttt ttgttttcgg
    40741 gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc ttcttgaaga aaatacttct
    40801 gcatggatca tcttaacagg tcttcttttt tgaagacgag tatatttagc accagtacca
    40861 ttgttatgtt cctctaatcg tctagaaaga tcggttgtat atccaccata aaatgtctga
    40921 tctttacaga ataatacgta aaaataatgt tcatttgcca tagagcatct cacgaatctc
    40981 tgcagtgtat tctccatcca tattgtaagt gatcaatggt ggagcgactt tgaatccgtc
    41041 taatttgcct tctttgattc cttcaactaa caaaatattt gcctcttttc caggttttgg
    41101 gtaaacaaat tggatccttt taggtgcaat tcgatagtct tccatgacct gaatgatttc
    41161 taagaaccga tcaggacgat ggaccatagt aaagcgtcca tttgttttta gtagcttact
    41221 tgaaatatcg atcacttctt ctaacgtcgt atgaatctca tgacgggcga ttgccaggtg
    41281 tttattaggg ttcgttttat ttgttggcag ccctttgaaa taaggtggat tacagacgac
    41341 taagtcacaa gaattatgtc gaacgacaga taagctgtct ttcagatcga tggtgtggac
    41401 agtcatttgt tcatttaaat ggttaagcgc gatactacgt cttgccatgt ctgctagacg
    41461 aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt ttacttaaaa aaagtccaac
    41521 tgcgccattt cctgcacaaa ggtcaacaat cttccctttt ttaggtacac gggaaaagtg
    41581 agcaagcagc acagcatcta aagaaaaaga aaacactttg gaactttgga taatcttgat
    41641 atcttctgcg tataattggt cgattcgttc gtcttcgtga agcatggatt ccctccaaaa
    41701 tgaaaaaaat agtcgtctgt attatactat ttttattaaa aagaaccaag gtggcttgtc
    41761 aaacctctat tttttatgca tactatgacc ataagaaggg aaaggaagag actatgtttt
    41821 atcgatttat gcgcggggta gttcgagtac tattagcctt agttaatgga aatgcccgct
    41881 atgagaataa agaagtttta ccgcaagatg agaattacat actagttgct ccccatcgta
    41941 cttggtggga cccattatat ctggcggtag ctgcagcacc caaggaattc agctttatgg
    42001 caaaagaaga actgttcaaa aatccagttc tgcgatttat attaactcga tccaatgctt
    42061 ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa aacacctgta aagattttga
    42121 aatcgacaaa tttgagtttg atcatgtttc ccagtgggac aagacatgct acagaactta
    42181 aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa aatcgtgcca gctgtatatc
    42241 aaggtccgtt gacacttggg gatctgttca agcgtcagag agtgacagtg cgtttcggcg
    42301 aaccaatcga cttatcagat atcaagaaaa cggataaaga aggtatggcg gagatcgaac
    42361 gacgaacaca agcagctttt gacaaattgg acaaagaagt gaaccctgat ttcaaatatg
    42421 aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg gtcttttgaa tctactgtcc
    42481 tgttcagttc agactttttc aatgaaaacg agggagagac gtcaagtctg ccctcttttt
    42541 gcgttccaat atgtaaaata gaagacaggt gtcatgtaat ggatcaaagt gttataataa
    42601 ttagaaggac attttttgag gtgattgaat gcgtttttta catacagccg attggcatat
    42661 cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag cgtcatgtac tatcttctat
    42721 actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg attgccggag acttatatga
    42781 taggagtgtc ccagctgttg atgccgtaga attatacaat gaattgatgg tcgattggaa
    42841 tttaaaggaa aagatcccga tttttgctat atcaggaaat cacgatagca gtacgagact
    42901 ttcagcgggt acgccatggt tcagttattc gaactatttt ttgcgaacac agctagcaga
    42961 agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc tttcttcctt actttgaacc
    43021 aattgcagcg agattatatt ttgaagatga caatatccga acgatcaagc aagcggtagc
    43081 caaggtgata gataaaatgc aggagctatt tgattcagaa aaaaaacaag tattgattag
    43141 ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa acgaaaatcg aagtaggcgg
    43201 gttggattcg atccctggtg aactacttac accgtttgat tatgtagcac taggacattt
    43261 gcataatcaa gctgctttga gacaggataa tgctcgctat agcggttccc cattgaaatt
    43321 ctctttatct gagatcaacc aaaaaaaagg cgtatggatc gttgacctga caacaggcct
    43381 ggagttgaat tttagggaga tcgaacccct gcatgatatt gaagaaataa cagcgagttt
    43441 caaagaattg ctggatcctg ccttttatca gtccgtcaat cgggagaatt acatctatat
    43501 ctatctcact gatagaacga tcatcccgaa tatgatgaac cagcttcggc aaatatatcc
    43561 tcgtattttg gggattgaac gggcaaacgg tcgagcagaa caaacaaagc gcaaacgaaa
    43621 agtactagaa atgaaagatc ctcaaaaatt agcagaagca ttttttagtg aaatgacgga
    43681 agagcagcta actgcaaacc aagaaaaatg gctcggagaa acgttgcaag aaatcatgga
    43741 aatggaatga tcaagaatga aaccgaaaaa attacaactt aaaaattttg gtccttttat
    43801 caatgagacc gtagattttt cacgtttgac agaagcaccg ctttttttga tcagcgggaa
    43861 gacaggagct ggcaaaacga cgatttttga tgggatcacc ttcgcacttt ttggtgaaac
    43921 ttccggaaga cttcgctctg gaaaagagat gcgttctttg tttgctacac cagacgaaga
    43981 aacaagcgtg actttttctt ttgaacatca gcagatgagc tatgaaatcg aacgaaaacc
    44041 agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa aaacaggcag ccaaagtaac
    44101 gttgaccatt tttgatggga aaggaaaaga aataaaacaa atcacaaaac gtacagaagc
    44161 agatcagttg atcagagaac tgatgaatct cgatgcgaaa cagttttctc aaatcgtttt
    44221 actaccccaa ggagaattcc gtaatttttt ggtttcatca agcagtgata aagagactgt
    44281 tttacgtcat ttattcggta ctcaattttt ccaacagttc aatgagcgat taaaagaaaa
    44341 agcaaaacaa cagcaacaaa aattggatca tttagagcaa gagctgcatc ttctgcaaaa
    44401 aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg tttgaagccg ttttagccca
    44461 atggacaaac catcaggcaa tactcgaaaa acaaatcacg gaagaaaaag aagctctaac
    44521 ggaacttcag gaaaaacaaa agcaactaga aaattcctac tatcgttttg aaagcttgaa
    44581 aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa aaggaattag ctgctcaaaa
    44641 agacgaaata gaggaaaaga aacgatggat tgggtattat gaatttacgc aacgattagt
    44701 cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa atagaactgt cacaaaaaag
    44761 tagtgagcaa acaaagaaac tagcgatgac tacgcagaat tatgaagagt ggcaaaaaaa
    44821 tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa gctgctcaaa aactgcaaaa
    44881 caaacagatg atggtgccaa ttgtcgaatc cttacgtagt aaaaaacaag acgcagaaca
    44941 attaacaatt tcattggata aagaagaatt gaatgccgag aagctgtatc aaaatctcgc
    45001 tatctatcag gaaagaactc gaattcttca agaaaattta ctggatcagg aaaaactgca
    45061 agaagatcgt cttgcttttg gacagttaaa atatctagat gaaaagcagc aagaaataga
    45121 aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata agagaagaac tcaaaaaagc
    45181 aactgaagaa ataacaaaaa atagtcagct attgaaagaa cagacagaac aagcagaaca
    45241 gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt cagctttttt tgcgtccagg
    45301 ggagccatgt cttgtttgtg gatcaacgga acaccccata caacaattaa aacacgaaca
    45361 gccaaataag gaagatatct taaaagtaga acaacaactg gcagaagctg agtctgctgt
    45421 agaaagaact cagcaaatca ttgccaaaag tgaagcacat gaaaataatc ttcaacaaca
    45481 attatcagat ttagaacaaa aaatcatggc aacgaatata caattggctg aacaagaaga
    45541 acacttgcaa caggctttga aaaaggcatt tcctgagata acagaacaaa aaacaaaaac
    45601 aactcttgta gtaatcgaac aatctttaga tagacgaaaa agcaagatgg ccaatgaaag
    45661 aatagagctg gaagaaataa gacaacaagc agaaaagctc gaacaacaat tagctgtcta
    45721 caaacagcag cagacagaaa aaaataatca gctccagcaa ctgcaaggag aaatcaaaag
    45781 tttagaacaa caattgcaag atacggatag taactgttta gaagaggaga tacgacgact
    45841 tgaaaaacag ttagaagaag actcgaatca actagctgtt gagcagaaag caggagtgca
    45901 attgttgcaa gatcttcata cgcttcggac acagcttgat cttcaacatg aacaaataga
    45961 aaaaatagat gaaaaaatag aaaaggaaca agaaagaata gctaaaaagt tagaaagcca
    46021 gtcgtatttt tcaacaattg aagaaatctg tgcatttgca caacagcaag cacaatataa
    46081 agaaaaccaa cgtacagtta cttcttatca tgaagagcaa ttagtgacag ctgaccggct
    46141 gcgtcaatta gaaaatatcc agctaactga gacattccca gatacagaag agatcagcca
    46201 acagttagaa gaaataagac gtatgatcga aataaagcaa gaaaagatat acggtttaca
    46261 agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt ctttcgattt atcacacaag
    46321 ccagacgaaa ttagatgagc ttacgcaatt acagcagctt tcgcaaacat tcaacggaga
    46381 aaatcctaag aagactagtt tggaacggta tgtgcttcag gtttatcttc aagaagtgtt
    46441 acaagtagca aatgatcatc tccaacgttt aacgaaaaat cgttatcaat ttgagctagc
    46501 ggataccatt ggcagttatc gagggaaaac cggattagaa atcaatatct atgatgatca
    46561 ggcaggtatg tctcgaagtg cgcataccct gtctggcggt gagagtttta ttgcagcact
    46621 ttctttagca ttagctcttg ccgatgtgat ccagacacaa gctggtggtg tagcaatcga
    46681 tgcattgttt attgatgaag gatttgggtc attagatgaa gaagctttgg aaatggcaat
    46741 ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc ggcatcatta gtcatgtaag
    46801 agaattgaaa gaacgagtat tacaacaaat aagaattgat acagaaggta gcggacaaag
    46861 caaaatacgc tatcaacttg gataaaaagg gagattggta actatgaaat ggagtattcc
    46921 agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa ggccgagtgt tatcggttgt
    46981 ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg ggcagtgaat tgtatatggt
    47041 tgatttggat ggtacagcaa aagagataga ttactgccaa tgtccctatt gggaggaaca
    47101 taaatattgt aagcatacag ttgctgtgga actttattta cggtcgaaaa atctatctcg
    47161 tatcatgaaa aaagaccagc caatgctaga accggtaaaa gcaacaagtg agggagaaat
    47221 cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat cctcaagcgg tgcaaccttt
    47281 gattattgaa taccaagtag aaacgattga gactaatcag tatcacccag agctttctat
    47341 tttggcactt tacttgcgta tcggttcatt ggcatcaccg aaaaaaacat atatcgtaaa
    47401 aaacatctat gattttttac aagcttacac agaaaaagaa acttacactg ttaataaaca
    47461 gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa gatcaaaaaa tactcacgca
    47521 tcttgcagga agtgctcaga cgcatcaatt attaggtacg aacggcttac aagtcaaagg
    47581 aaaactagat aaaagatatc tgcttctccc agtagaacaa gcccgcttcc tgttagagca
    47641 gatgaatcag actacacgtt tgcatctgca gataaatgaa caaaacgatc aaggaataca
    47701 attttctgat caaaacaaac ctttatcatt caaagtggaa aaatttggag aagattattt
    47761 gctgcaagca ttgaatgatt ttgatttttt ctttatgcat tatcagtggg gagttatcca
    47821 agggacattt tattctctga cgaaatatca acaaacggtt tatcaaactt ggaaacaatt
    47881 gattcgccga ttggagaaac cagaagtagt gttcaagaaa aaagaattac cgtctttgtt
    47941 taaagagatt atccctctgc tgtcagagat cggcgacgta gctattgccc ctgcagtaga
    48001 agaggaagta gcagatatcc ccgtcgaatt tattttcttt ttccgaaaag caaaaggaaa
    48061 aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc tattcgactg acccgaagca
    48121 tgaggtctcg acagaacagg cttttcaggt gttacgtgac acagtagaag agaaacgagt
    48181 acttgatcta ttcagaatgt atcgctacca gaaaaatgaa actggttatg aacgtccatt
    48241 gccaagcggg gaagaactat atgccttttt ccgcacagaa ctatctgtct tccgtcagtt
    48301 tggtgaagtc agacttggaa gaaaattgcg agagttgtat ttagatgcgc ataaacatca
    48361 accgaaaatc gaagtaaccg agaccggttc atggctcgat attcgattcg atgtgacagg
    48421 gatagaggag caagaaatcg atcatgtcct gcaaagcttg ctgagaaatg atgcttttta
    48481 tacattagaa aatggtcagg ttctttcttt tgattcagaa gaatttcaac aaacaagcca
    48541 agtgttgcaa caattacggg aatcgatacg tacagaagaa ggaacgatcc atgtacctaa
    48601 aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc aatgctactt ttagtgaatc
    48661 gttccaaacg atggtacaag atctcattca tccagaaaga taccaggcac agcttcccaa
    48721 aggattgaat gcaacgatga gagattatca aaagcaaggt tttcgatggt tgaagatgtt
    48781 aggtcattat cagtttggcg gtattttagc cgatgaaatg ggacttggga aaacactaca
    48841 gacaattgcc tttttacttt cggaaaaaga agaacggaaa agcttctctg ctttgatcgt
    48901 agcgcctgcg agcttaattt acaactggca ggcagaggtg agaaaattcg ccccttcttt
    48961 aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa gaattactgg ctaaagacac
    49021 agatatccga gtgacttctt atgccagctt aagacaggat ctagctaatt atcagtcgca
    49081 aaaaattgat tatttgattc ttgatgaggc acaaatggtc aaaaatagca gtacgaaaac
    49141 tgcacaagca ttgagagaat tagctgtacc tcagcggttt gcactgagtg ggacaccaat
    49201 cgagaataat ttagaagaat tgtggtcatt gtttgcaacg attatgccag gatttttccc
    49261 gactaaaaca aaattcaggg aacttgccgc agaagaaatt gcgcaaatga ttcgaccgtt
    49321 tattttacgg cgagataaac aaactgtgtt gaaagacttg ccggaaaaaa cagagatgaa
    49381 tctatattct gcacttacag aagaacaaaa aactgtttat ttagcttact tgcgtcaaat
    49441 gcgtgaagaa atcacatcaa tggattctga agcattcaag aaaaaccgga tcgggatact
    49501 tgctggtctg acaagactgc gacaaatttg ctgcgatcct cgtctgttta ttgaagatta
    49561 ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta ctggtagctg ccaaagagaa
    49621 caaacgaaga gttttgctgt tttctcaatt tacagggatg ctgactatct tgcaagaaga
    49681 gttagccgag ctagggatct cgacatttta tttaagaggc agtacgaaac cacaagatcg
    49741 gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat gtttttctga tttccttaaa
    49801 ggctggggga actggattga atctcacggg agcagatacg gtcatcttgt atgacctttg
    49861 gtggaatcct gccattgaag aacaagctgc tggacgtgca catcggatcg gacaaaaaaa
    49921 tgtggtagaa gtgtggcgta tgatctctga aggaacgatt gaggaacgca tggattcact
    49981 ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa ggtaatgaag aacagttgac
    50041 gaaactgact gaagaagaca tccgcatgat tttaagtgta ggagaaatcg aggagtaaga
    50101 gactcgaaaa agcgttcagt aatgggtaag aaagcctttc taaagaagcg attgtttatt
    50161 tgatttaaaa aatagcaagg aggctgggat tccattttat cccagcttcc ttgctatcat
    50221 ttttttattt ctatttttaa aggttataca aagattgtct tcattgtctc taacagctga
    50281 tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt caattttgcg ttctcgatgg
    50341 ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta gaacatcatt gtcgtaatta
    50401 tcgccaacgt ataaggtctc ttctggaagc atatgaaatt ctttctcagc cagctgaaaa
    50461 atctcttttt ctggtttttg gaaacctgtt gcttgagaaa tgagcatatt gcgggaagga
    50521 atccaattgt tcaattgaag ttgcttcagt ttttttgtct gatggtcagt cggcccattt
    50581 gtgataattc ctaatgggac attcttttcc ttcaagaaat cgagggtttt tttgacctcc
    50641 tcatgcaagc agatattatc aagttcttcc tcgtaaattt tttgaaataa gaggccatct
    50701 tcttccgtaa tatgagggta gtctaaatct tttagtgatt ggctgatccg atgtgctcgc
    50761 atatattcta gtgtccaatc tcctgccatt acttttggga agttttcatc actatgatgg
    50821 cggaaccgga tatatagtgg atgcatatct gcatctgaaa caagcggaac gactcttttg
    50881 accgcgtttc gaaatggctg ctgctgatca tacatcgtat catcaacatc aaacacaact
    50941 gatttaatca aaattttaca cgcctttcgg ttttcattga ttttttcaca aatccttgac
    51001 tattgtaaca gaaaaaattg tctaaaaaag ctgataaata aaggtttttc ttactatatt
    51061 tctatttcct tgacagaata aggtttttat atttcagttt ttttcactaa aaatcgtgaa
    51121 acacgtcaat aagtttgatg aacaaattaa tcattatggt agaagtcgat tgatttattt
    51181 taatgcaatc tctgtggaat tttcgaaaga tttat
//
LOCUS       DAZULO010000009        50113 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000009 DAZULO010000000
VERSION     DAZULO010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.009"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1399
                     /locus_tag="ACG8OA_000560"
     CDS             <1..1399
                     /locus_tag="ACG8OA_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFS4975403.1"
                     /translation="IYTGINAAQVFGATPTLGGVIGAVVMLTGMNPEAPLKNLFTGEA
                     LAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIVTPTLSLIAIGLIEIFFIMPLA
                     GFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFGLHQILTPIHVQMIDETGRTLL
                     LPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALPVGILGIGEPLIYGVTLPLGRP
                     FITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLISDGKWLGYILGLLAAYAGGFV
                     ATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSAESVTILSSVADGTVVSLDKAS
                     DPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTKHAIGITTPNGLEILLHMGINT
                     VDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGKETSMIVLVTNMDRVKNFVLEK
                     TGKAKAKAPVMDVKA"
     gene            complement(1464..1910)
                     /gene="nrdI"
                     /locus_tag="ACG8OA_000561"
     CDS             complement(1464..1910)
                     /gene="nrdI"
                     /locus_tag="ACG8OA_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS4975404.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            2121..3545
                     /locus_tag="ACG8OA_000562"
     CDS             2121..3545
                     /locus_tag="ACG8OA_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFS4975405.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(3726..4499)
                     /locus_tag="ACG8OA_000563"
     CDS             complement(3726..4499)
                     /locus_tag="ACG8OA_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4975406.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(4496..5401)
                     /locus_tag="ACG8OA_000564"
     CDS             complement(4496..5401)
                     /locus_tag="ACG8OA_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4975407.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(5555..5875)
                     /locus_tag="ACG8OA_000565"
     CDS             complement(5555..5875)
                     /locus_tag="ACG8OA_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFS4975408.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            6349..7035
                     /locus_tag="ACG8OA_000566"
     CDS             6349..7035
                     /locus_tag="ACG8OA_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFS4975409.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            7037..7981
                     /locus_tag="ACG8OA_000567"
     CDS             7037..7981
                     /locus_tag="ACG8OA_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFS4975410.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            8180..8824
                     /gene="pcp"
                     /locus_tag="ACG8OA_000568"
     CDS             8180..8824
                     /gene="pcp"
                     /locus_tag="ACG8OA_000568"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFS4975411.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            8903..10180
                     /locus_tag="ACG8OA_000569"
     CDS             8903..10180
                     /locus_tag="ACG8OA_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975412.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10364..11113)
                     /gene="pnuC"
                     /locus_tag="ACG8OA_000570"
     CDS             complement(10364..11113)
                     /gene="pnuC"
                     /locus_tag="ACG8OA_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFS4975413.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            11443..11823
                     /locus_tag="ACG8OA_000571"
     CDS             11443..11823
                     /locus_tag="ACG8OA_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4975414.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11919..12428
                     /locus_tag="ACG8OA_000572"
     CDS             11919..12428
                     /locus_tag="ACG8OA_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975415.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            12505..12684
                     /locus_tag="ACG8OA_000573"
     CDS             12505..12684
                     /locus_tag="ACG8OA_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975416.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            12739..13302
                     /locus_tag="ACG8OA_000574"
     CDS             12739..13302
                     /locus_tag="ACG8OA_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4975417.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(13349..13864)
                     /locus_tag="ACG8OA_000575"
     CDS             complement(13349..13864)
                     /locus_tag="ACG8OA_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFS4975418.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(13890..14759)
                     /locus_tag="ACG8OA_000576"
     CDS             complement(13890..14759)
                     /locus_tag="ACG8OA_000576"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4975419.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            14885..15565
                     /locus_tag="ACG8OA_000577"
     CDS             14885..15565
                     /locus_tag="ACG8OA_000577"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS4975420.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            15718..16698
                     /gene="pta"
                     /locus_tag="ACG8OA_000578"
     CDS             15718..16698
                     /gene="pta"
                     /locus_tag="ACG8OA_000578"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFS4975421.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            16821..17294
                     /gene="tsaE"
                     /locus_tag="ACG8OA_000579"
     CDS             16821..17294
                     /gene="tsaE"
                     /locus_tag="ACG8OA_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFS4975422.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            17287..17811
                     /locus_tag="ACG8OA_000580"
     CDS             17287..17811
                     /locus_tag="ACG8OA_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFS4975423.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(18301..18834)
                     /locus_tag="ACG8OA_000581"
     CDS             complement(18301..18834)
                     /locus_tag="ACG8OA_000581"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFS4975424.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(19698..20450)
                     /locus_tag="ACG8OA_000582"
     CDS             complement(19698..20450)
                     /locus_tag="ACG8OA_000582"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFS4975425.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            20563..21483
                     /locus_tag="ACG8OA_000583"
     CDS             20563..21483
                     /locus_tag="ACG8OA_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4975426.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            21556..22491
                     /gene="murB"
                     /locus_tag="ACG8OA_000584"
     CDS             21556..22491
                     /gene="murB"
                     /locus_tag="ACG8OA_000584"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFS4975427.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            22789..24267
                     /locus_tag="ACG8OA_000585"
     CDS             22789..24267
                     /locus_tag="ACG8OA_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4975428.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            24544..26604
                     /locus_tag="ACG8OA_000586"
     CDS             24544..26604
                     /locus_tag="ACG8OA_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFS4975429.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            26687..27256
                     /locus_tag="ACG8OA_000587"
     CDS             26687..27256
                     /locus_tag="ACG8OA_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4975430.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(27431..28237)
                     /locus_tag="ACG8OA_000588"
     CDS             complement(27431..28237)
                     /locus_tag="ACG8OA_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975431.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            28375..29889
                     /locus_tag="ACG8OA_000589"
     CDS             28375..29889
                     /locus_tag="ACG8OA_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFS4975432.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(30292..31275)
                     /locus_tag="ACG8OA_000590"
     CDS             complement(30292..31275)
                     /locus_tag="ACG8OA_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFS4975433.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(31280..31927)
                     /locus_tag="ACG8OA_000591"
     CDS             complement(31280..31927)
                     /locus_tag="ACG8OA_000591"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFS4975434.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(32012..32644)
                     /locus_tag="ACG8OA_000592"
     CDS             complement(32012..32644)
                     /locus_tag="ACG8OA_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4975435.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(32660..33211)
                     /gene="coaC"
                     /locus_tag="ACG8OA_000593"
     CDS             complement(32660..33211)
                     /gene="coaC"
                     /locus_tag="ACG8OA_000593"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFS4975436.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(33208..33945)
                     /locus_tag="ACG8OA_000594"
     CDS             complement(33208..33945)
                     /locus_tag="ACG8OA_000594"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFS4975437.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(33942..34931)
                     /locus_tag="ACG8OA_000595"
     CDS             complement(33942..34931)
                     /locus_tag="ACG8OA_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS4975438.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            35189..35731
                     /locus_tag="ACG8OA_000596"
     CDS             35189..35731
                     /locus_tag="ACG8OA_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFS4975439.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            35748..36833
                     /locus_tag="ACG8OA_000597"
     CDS             35748..36833
                     /locus_tag="ACG8OA_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4975440.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            36845..37651
                     /locus_tag="ACG8OA_000598"
     CDS             36845..37651
                     /locus_tag="ACG8OA_000598"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4975441.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLLLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            37652..38479
                     /locus_tag="ACG8OA_000599"
     CDS             37652..38479
                     /locus_tag="ACG8OA_000599"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4975442.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            38476..39549
                     /locus_tag="ACG8OA_000600"
     CDS             38476..39549
                     /locus_tag="ACG8OA_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4975443.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            39977..42673
                     /locus_tag="ACG8OA_000601"
     CDS             39977..42673
                     /locus_tag="ACG8OA_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4975444.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            42738..43238
                     /locus_tag="ACG8OA_000602"
     CDS             42738..43238
                     /locus_tag="ACG8OA_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4975445.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            43250..43744
                     /locus_tag="ACG8OA_000603"
     CDS             43250..43744
                     /locus_tag="ACG8OA_000603"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4975446.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            43765..44574
                     /locus_tag="ACG8OA_000604"
     CDS             43765..44574
                     /locus_tag="ACG8OA_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4975447.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            44571..45392
                     /locus_tag="ACG8OA_000605"
     CDS             44571..45392
                     /locus_tag="ACG8OA_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4975448.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            45443..47542
                     /locus_tag="ACG8OA_000606"
     CDS             45443..47542
                     /locus_tag="ACG8OA_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFS4975449.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            47539..48657
                     /locus_tag="ACG8OA_000607"
     CDS             47539..48657
                     /locus_tag="ACG8OA_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFS4975450.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            48696..49574
                     /locus_tag="ACG8OA_000608"
     CDS             48696..49574
                     /locus_tag="ACG8OA_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4975451.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            49720..49917
                     /locus_tag="ACG8OA_000609"
     CDS             49720..49917
                     /locus_tag="ACG8OA_000609"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS4975452.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            49901..>50113
                     /locus_tag="ACG8OA_000610"
     CDS             49901..>50113
                     /locus_tag="ACG8OA_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975453.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILI"
BASE COUNT        16034 a         8740 c        10408 g        14931 t
ORIGIN      
        1 tatctataca ggtatcaatg ctgcacaagt ttttggtgca acaccaactc ttggtggagt
       61 tatcggggcc gttgtgatgc tgacagggat gaatccggaa gcaccactga aaaacttatt
      121 tactggtgaa gctcttgcag ctggacaagg tgggatccta ggtgtcatct tcgctgtttg
      181 gctattgtca atcgttgaga aaaaactgca taaagtagta cctgatgcgg tagatatcat
      241 cgttacacca actctttcat tgattgccat cggtctgatt gaaattttct tcatcatgcc
      301 tttagcaggt ttcatttctg atggcatggt cggtgggatc aactgggtac ttggcgttgg
      361 tggagcattt gccggcttcg ttctaggcac attattcttg ccgatggtta tgttcggact
      421 gcatcaaatt ttgactccaa tccatgtgca aatgattgat gaaactggtc gtacgctatt
      481 gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt gcagcattgg cattatggat
      541 tcgctgcaaa aaagataaag aattaacaga aatgatcaaa ggagctttac cagtcgggat
      601 cctaggaatc ggcgaaccat tgatttacgg agtaacatta cctctaggac gcccatttat
      661 tactgcttgt atcggtggag gtatcggcgg ggcagtgatc ggaatgatcg gaaatgtcgg
      721 ggctatcgct attggacctt caggagccgc tttgatccca ttgatctctg acggtaaatg
      781 gttaggctac atccttggat tattggcagc ttatgctggt ggcttcgttg ctacattctt
      841 cttcggtatt cctaaagacc gtttagaaaa agaagaatta gcgactgaaa cagccacagt
      901 aaatgaagtg cctgaaaaag tagtacctca aacttcagct gaaagtgtga ctatcctctc
      961 atcagttgct gacggaacag ttgtttcttt agataaagca agtgatccag tttttgcaca
     1021 aaagatgatg ggtgaaggct actttgtaga accagaaaac ggacatatct attctccagt
     1081 tactggggta gtcagctctg tcttcccaac gaaacacgct attgggatca caacgccaaa
     1141 tggtttagaa atcttgcttc atatgggaat caacacagtt gacttaggcg gtacgccatt
     1201 tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat acgcttgtag cagaagctga
     1261 cttagaagcg atcaaagcag caggcaaaga aacatccatg atcgtcttgg taaccaatat
     1321 ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa gcaaaagcaa aagcacctgt
     1381 gatggatgtc aaagcttaat atgatagaaa caatagaaac gaaagaagaa actgaaatct
     1441 atttttcagc ttcttctttt ttattattta ttgttttctg ctaaaatctg ataaattcgt
     1501 gtcacatcag atggtgttcc ccgtaattca taatctgcca agaaaggaaa gccaaatgtt
     1561 tgtgcatatt gtttggctgt tagacaatac tgattattga agtttttatt tccgctaccg
     1621 actacaccaa gacaaagttt atgattgtct tcatgttcca gatattcacg catcgtttcg
     1681 gtcaagattt ctgtatcacc gttatctaaa ccatttccgc cgtctaaata agtaggaacg
     1741 aaagtaaaaa atggttctgt ttctttcgta aaatcactat tttcatggat ttcttttaaa
     1801 gtaacttcag gattgacagg atcttctgca tgcatttttt cagcatattc tgctagatgt
     1861 ttggcaaagg cacgcgtatt tcctgaaatc gatatatata aaatgttcat ggatcatcct
     1921 ccaattcttt ccttttagta tagcatcttt tttaagtttt ttggtggtgt aatttggaaa
     1981 gggactaggc gctttgcttg tataatgaga aaaaagccgg tagaattata tttctattaa
     2041 agtttttatt aattaagtaa aaataaaaaa ggtttgttat actttttgat aaaagcggtt
     2101 ttattttgga ggatgcttac atggatacag agcaacagaa aaaaggaatt ggtttaattc
     2161 cacttgctgc cttagtaatt ggttcagcga ttggaggtgg cgtatttggt attatgacgg
     2221 acatctccgg atcggctggc ggcccagcta ttttcagttg ggtactcgta ggaacagcaa
     2281 tgttgatgct ctccttgtcg atcaataata tcaataaaaa acgtccagat ttaaaaggcg
     2341 ggatctttag ttacgcagaa gaaggttttg gccgtttcgc tggttttatc agcggttggg
     2401 gttactggct cacatgctgg ttaggcaacg tagcttttgc aacattgttg atgagcgcat
     2461 taggatattt cttcccagta tttgaaggtg ggcaaaatct ggcatcgatc gtgatgagta
     2521 cagtcttttt atggggatat gcatggctag tgaatcgggg agtcgaaaat gctagtttcg
     2581 tcaatgctat cgtcacattc tgtaaattag tgccattatt tttatttatt gtagtagcta
     2641 tcagcacgtt ttcgatccaa caattcattg ataacttttc tagcgacatt atattgaatg
     2701 cgaatggaca gccgatcccg ttttccagtc aagtattgag ttgcatcatg gtgatgcttt
     2761 gggtatttgt tggtattgaa ggagcctctg tcgtaggttc acgagcaaaa aaacaatcag
     2821 atgttgggaa agcaacgatt ctagggatat tcggcttgat tattatttac gtcttggttt
     2881 ccatgcttcc atatgggaca atgacgatga atcagctgca aacgatcaag gcgcagcctg
     2941 ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg agcagtcgtg atcaacggcg
     3001 gtttgatcat ttctttagtt ggtgtttggc tctcatggac gatcttaccg attgaaacaa
     3061 tgggaaacat ggctgaagac ggacttttgc ctaaagcatg gggaaaactc aacaaaaatg
     3121 gggcaccaac ttatgctatc gtattgacga cgttatgtac gaatgtcttt ttactttcat
     3181 tattattcac tgacagtgct tataactttg cttacacact tggtacagca gctattttct
     3241 tcacgtggct tttcttagga ttgtaccaaa tgaaactttc ttatcagcgg aaagaatggg
     3301 tccaattttt agtagggctt ttagcaagtg cctttcaagt atgggctatg ctagtcgtag
     3361 cctttaatga agtaatgctt gttcttgttc ttttcttgcc aggcatttat ttttacctca
     3421 aagcacgtaa ggagcaaaac gagcctgtac aacatatcca cttagataag attttactta
     3481 taggtgtgac gatggtcgga gtgattgcaa tggttttatt tgccactggg atgcttcacg
     3541 tatagaagta taaaagaagt ataaagagag gttgtgagcc tgtcgtttat tcaaaaacat
     3601 actaggaaag gagctgtgac acaaatgttg tcacagctcc tttttcagaa taaacggtgt
     3661 ttaagagaca gccaatcggc aataaggctg aacgtctctt tcacagcctc ttttttgatg
     3721 agtcatcata cattttttcg cttaaatacg agatatccta atgctaagaa caaaacgatg
     3781 taagctaaat taccgaatag catttgatta gtagaaagat gagtcaattg cttcatgatc
     3841 tcttcgtttc caatttggtt ttgcagattc aacatactga tcggattcca tttgatccac
     3901 tcccattttt ggatgagtgc aataaggata ccagatatga ttgacgaagc aaaataaaag
     3961 acgattcctg ctgagatgga tgcgccggaa ctattgataa agcaagcaat catcaacact
     4021 aaacttaaaa tcagccataa accgatataa ctacccaaag tataagtgaa caagctttgg
     4081 atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa tcgaagggaa aagaatcagt
     4141 ttcatcaaca cagtgacaat aatagctaaa aggtataaat aaacagaata aataactaat
     4201 gtgatccatt tgcttactaa tatttctcct cgagaatact ttcgataaag gagatttttc
     4261 aatgtaccat attgtgcttc catcgaaatg atcgtgcttg cagctgcaat catgataaag
     4321 acaatccaag atgtggcact aaaaagctga gcagtcattt caactggatc aatgatcgtc
     4381 gtgtattttt tggctagaag tgctgttcct atgagcaaaa caactaatag aacagataaa
     4441 acagcagttg actttttctt gatcaattta aataattctt gttttacaag caatgtcatt
     4501 tttcttcttc cccctttact gaatccagaa tttccaatag agaagattcc aaatcattcg
     4561 tcagatgctg gacgtctgtc acatggatat tttgtgcaga caatagctgg atcacggttc
     4621 ctaataattc ttcgttttct tttaaaagct tgattttatc aggatcttcc aaagtatatc
     4681 cagcttctgt caagatcgtt cgagcagcta gatcatttgt tgtagagata acgatgaaat
     4741 cttgcgctgt ttggttcaat tcagccattg tacattgttt gatgatcttt ccttgattga
     4801 tcaggatcag ctcatcgacc attttttcaa gctcgctgag taagtgactg gagatcaaga
     4861 aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg cacatctctt gttgcttgtg
     4921 gatccaaacc gttcatcggc tcatctaaaa taacgataga cggatggttc agtaaggcaa
     4981 gagcaattcc caatttttgt ttcattccca acgagtagga tttcgctttt ttgtgaatgt
     5041 acttttccat cttgagctga ttcacgatag tgtcgatagc agatacatcg ttggtttcag
     5101 agaataactt cagatgatca tagcctgtta agaatggata gatacccggg tattcgatca
     5161 atgctcctac ttgttccaac cctttatgac tagagggaga aatctcattt tcaccaatac
     5221 agattgttcc atctgagtaa tggatcagtc ctaaaatagc tttcatgatc gttgtttttc
     5281 cagcgccatt aggacctact aatccaacga ttttgccagg ggataagcta aaactgacat
     5341 cgctgagggc ttgtttgtga ccaaagttct tggataattc ttggaccgaa agacttttca
     5401 aaaatattca ctccttttct atagatagta caagcataca cctgatgagt ggagaagtcg
     5461 ctaggtcatt ggacttattt cttatttttt cataaaaaaa ttgaatatta aataaaaagg
     5521 cttatttttt ctgatcagta cttattccaa gtgtctagtg gccgctagta atcttgatgc
     5581 caatgatact gatgagtagc atccccacaa agaaccaagt ggaaagattc agcttatcat
     5641 gaaacaagac cacaccaatt atgaccgaac caactgcgcc aatccctgtc cagataggat
     5701 aagagagact catcggcaaa cttttgattg cctttgacaa aaagtaaaaa ctagcaatca
     5761 tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa accatcactc catttcatca
     5821 tcgttgacca gaatacttcc aaaattcctg ctatgattaa ttccatccat gccatactta
     5881 cattcctcct taactataaa aaaatacaca aaaagaggaa aatcatccct tcaaagacct
     5941 aatggaatga tcatcctctt tatcctaccc ggtggcagat tgtatatctt tttctgtagt
     6001 atagcacacc tatccagata tcggcaatct catacttttt agtaaagtgt accaagcact
     6061 gacggatata tcgttagcgg aaaagaagat gaatgctttg caatttcata gaaagaagtt
     6121 gttatttatt aatcatttaa aaaaaacagt aactagacct tatttctata tattatcggg
     6181 caataaatta aaaaaagctt taccttacaa taagtaagat aatttttgtg ataaaaaaat
     6241 aacgaaaaat atttaaaaat agtgtctatt tcgtgagaaa acaactatat tttttatgtg
     6301 ttttgcttta taattaaaaa aatattgtca ttttatggag gaaaatatat ggaaaattat
     6361 cttagccttt taggcgtctt gattgttatt ctcgggtttg catttaaatt agactcgata
     6421 ttgatcgtaa tggttgcttt agttgttaca gctttgactg gcggtcttgg cattcaagga
     6481 atgttagaac ttttaggtac aagttttgta aacaatcgcg ggatggcgat ttttatcatc
     6541 attatgttgg caacaggaac gcttgaaaga aatggtctga aagaatcagc ggcttccttg
     6601 atcaagcgat tcaagaaagt atctgctggt atgattgtag atatttatgg gatctttcga
     6661 atgatctttg cggcttttaa cgtgagtttt ggtggagttg ctggatttgt tcgtccaatc
     6721 attttaccga tggcattggg gaccatcgaa tcaaaagggt tagaagtcca tcctaagcat
     6781 gaagaagaac taaaaggtat ggcttcagct atggaaaatg tctgctggtt ctttggccaa
     6841 gtactattca tcggaggagc aggggcgttg ctagtccaat caacactgaa agatctagga
     6901 tatgacgtaa cattgggaca gcttgcttta gtggaagttc cagtggctat cacggcttta
     6961 gttgtggcaa gtgtctactt ctatcttaaa gaaaagaaat tagcgaagaa atattatcca
     7021 ggaaaggggc aataagatgg acttttttac aaatcctgat attttattag gcgataaatt
     7081 actggagatc ctttatatcg tgatgggact cgtattgatt tacactggag taagaaattt
     7141 aacagataaa accaatcctc atcgttatgg gtcagccttt ttctggacgg tcttaggggt
     7201 agttattgca ggaggaagat ggcttccggc aatcgtgaac ggtggcttga tctttgcgat
     7261 gaccatacca gcaattgcta agaaagtcag caaaggagaa agccggcttc catcaaaaga
     7321 atatatggaa aaaatgtcag acaaactagg aatgaaaatt tttattccag ctttgagtat
     7381 cggtgtcttt gcgatcttgt ttgctctatt taccaattta ggtgctttag tcggcgtggg
     7441 cgtcggtgtt tttgcagcca tgctaatctt gatgttcttt tctcgagata atcgaccaag
     7501 tacattttta gatgatgcag cagatatgct gggcacagta gggccgttaa gcatgttgcc
     7561 aatgctttta gcttctctag gtgcggtatt tacaagcgct ggagttggga cagtgatttc
     7621 caatgcagta ggtacgatcg ttccagaagg aaatgtgaac atcggcatca tcatttacgc
     7681 tcttggcatg gtgatattta cggtaatcat ggggaatgca tttgcggcca tcaccgtgat
     7741 gacggttggg atcggtgcac cattcgtatt tagtcatgga gcgaatccag cgttaatagg
     7801 aatggttgca ttgacttgcg gtttttgtgg gaccctgctg acaccgatgg ctgctaactt
     7861 caatatcgtg cctgtagcga tgctggagat gaaagacaaa tacggagtga tcaaaaacca
     7921 gttgtttatt gcgttattta tgctagtttt ccaaattgct tatatgattc tatttaaata
     7981 atagaagaga ggggttgtga cagaataatt gtcgcaacct cttctaacat tgtagtggaa
     8041 cactattttt ttataagaat atattaagtt tgatctgtta tgttatttaa actattgttt
     8101 cctttattta cgctgttcaa ggaaaatggt atgataaaag ataaccatat gttaaatcaa
     8161 tcggacggag gaacaacaga tgaaagtact agtaacaggt tttgatccat ttggtgggga
     8221 caaagtgaat ccagcctatg aagcagtaaa aaaaatgcca gatgaaatca gcggtgctga
     8281 aatcatcaaa gtggaagtac cgaccgtatt cgaaaaaagc agtcaagtag tcaaagaagc
     8341 catccaacag caccaaccag atgtcgtgat ctgtgtcgga caagcaggtg ggcggtcagc
     8401 agtctcgttc gaacgtgtcg caatcaatct agcagaagca agaatccctg ataatgaagg
     8461 aaatcagcca ttcgatacgg ctttagaaga aaacggtcca gccgcttact ttacctcatt
     8521 gccaatcaaa gcaatgacaa aaaatgtaca agatcatggt ctaccagcgt acatctctta
     8581 tacagcagga acatttgtct gcaatgatat catgtatcgt ttgcttcatt tgatcgaaac
     8641 acaattcccg gcaattcgag gtgggttcat ccacgtgcca ttctcacccg atcaagtgat
     8701 cgatcgtcca gtgggtacgc cttctatgtc tttagaagac attgcttctt ctttgactta
     8761 tgcagttgaa gcagcagtag aaaataaaga agatatttcc ggaaatgccg gaacaactca
     8821 ctaatttagc aagaaacact ggctggttgc taacgttatg ttagtagcca gctttttgta
     8881 attagaaagg aaagaaacag ccgtggaaga atggcaatcc gtctttgaag aatggtttcc
     8941 aaaagaaatc agcaaaagct atcccatcaa aatttctaaa caatacacaa gcagtcaacg
     9001 ttgggagatc tatgcaaaat taacgaaaaa acagcgggaa ctagtcgata agcaccgccg
     9061 atatctaatc agctctcgtt ttatggaaga acattattta gctgcaacag attgggtgtt
     9121 ttctgacttc aagatcaatc cgttttttcg aacaaaacga agccagcaaa aattgtattg
     9181 tgagtgtggt agagaattaa aagtgcaata catcgttaaa tcgccaaaaa ccggaaagat
     9241 cctaaaacta ggcatcaacc actttgccga tcacctgcat gtctctccga cagtggcagc
     9301 ttcgatccat cagggaatga caaaagttga tctggcctta gacgaattat tatggcttaa
     9361 gcagaagaat atcgattttc ctgaaggact atggcaaaaa tactgttttg tcttgtacca
     9421 aaatcgtcgg atgaaacagc catatttacc ggatataaaa ttagctcagc ggctcgcaga
     9481 atttcgccaa gtagaaatgc cgatttacat agcggattat caagcattgg aaaatgaaat
     9541 caagaaaata agtgaacaca tcaatgggca gtcaaaaaag cgacagataa aaaaagaact
     9601 atttgacgac tttgcagaag aattagtaaa ggatgtagaa gaattcttga tcaactatcg
     9661 cgcattttta cgtaaagatt ggcaatccat cgtatatgaa gaagtgccag tacatcctaa
     9721 tgcttatttt gaaacattta tttccgtgtt gagaaagaca aaacgtcaac gaacaccaga
     9781 agtcactgct cagatggaat attttgcgaa aaaccaacga ttcatccaac caaaaatcta
     9841 tctatttatt tggaagcaat attgtcgcta tggttttaca gaagggttct tcgacagcat
     9901 tcctcggata gtcagaaatg gttttttaaa agtattgcgt aaagaacgag aagctataca
     9961 atctgctgat aagaaagacc gtacagtctc aaaagaaaaa tggcaactag tagtgaaaga
    10021 tatccaatct ggaaatgtgc aagagacaat cgacaaatgg aaaggaaaac attatcgttt
    10081 tactgaagct cagaaacaag cgttggaata ttatcagaag ttagaagaat cactgcgttt
    10141 caatgatgag gcaagaaagt atctgaagga attactataa aaaatagagg ctgggacaga
    10201 agcgtttaac tccaagaaat aagaagaaat tcacgaaaat tgcttttcaa atttttgtga
    10261 atttcagctt atttccgaag gagttgcttc tgattccgcc gtttatacgt gtttagagcg
    10321 tgagacaaaa gtgttcttta cttttgtccc acgctcagtt tttttatgcg tgttgttgtg
    10381 cgttgtagta ttcttcttcc attttttgca tacgttcacg ttctttttgg ttataccaag
    10441 gtgaaacggt aaatgcaagg acatcattca gcaagtaaac agagctgttc acgaacatcg
    10501 ccaaacttgc agatccatgc gtaaatgaaa tataccaaag gaccatttga gcaagccctg
    10561 aagctaacca ccagtaatat tggttgttgt aacgcaagaa actgatgata ccagcagtca
    10621 agctaataga aaaagcaatg gcatctaccc acggccgagg atcatctgta taacgaccaa
    10681 tcaggaatcc tgaaacaaga tagacaagga aagtactgat gattgcgata aaccagcttt
    10741 ttccagtgaa ttttcggatt tttgaagcca tattatgatt ccattcttta cttaacaata
    10801 cggggatatc caatgtgatc atataagcaa tctgttcacc gatacttaaa tagttctttg
    10861 ctgaaaaccc aacgataata aaacaaatcg cagacaaaat acctaaaata ccgttgactg
    10921 attttgccgc attgatggat aaaatacaca atacaccaag cgtggtaccg ataaatgtaa
    10981 taacggacag ccaagtgatg ggactattga caagagtcat gacctggcag ccaagactga
    11041 aaaagaaaag ataataattt tgttgcggcc agccttttag ctgatgagct aaaaactgaa
    11101 aatatgactt catgtatgag gactccttaa gacactaaat gtagtgtgtt ctgtatttac
    11161 taacacaatc tattgtatgc ttttcttgaa aaaaaactag tctttcaatt ataaataatt
    11221 tgtgttatga taattttctg tttgttttct tgaaaatata atttcttact ttcatttatt
    11281 atattgggaa caggaagagt gtcttggagg acctactttc tgtttttttt gccttttttc
    11341 ttataaaaag gaaaaacagc tatttttttc aatctgacac atttacaatg gaactggaga
    11401 ttaatataga tccgagctta aacgaagaat aggagtgctc atatgaacct tacaacgatc
    11461 catcatatag ccatcattgt ttcagattat cagaaatcac gtgattttta tgttaataaa
    11521 ttaggatttg aagtaatcag agaaaattac cgcaaagaac gagatgacta taaattagat
    11581 ctgaaactag gaagtgctga attggagatt tttggaattg ctaatgcgcc acaacgacca
    11641 aactatccag aagcttgtgg cttgcgtcat cttgctttca aagtagacga tattgaagaa
    11701 acaattgcag aattgcagtc cttagggatc gaatcagaac cgattagaga agataccttt
    11761 acaggaaaaa aaatgacgtt tttctttgat ccagacggat tgccattaga actgcacgaa
    11821 taaagataac aaaattgtca cttttttttc gtttcattcg atttttcatt cgttcatttc
    11881 ttgatattct ttaaaaaaaa gaaacaggag aatgaagaat gaaaaaaatg atgtttggtg
    11941 ttgctgccct catcgtaata gggattaccg gtatgacggg actgctcaat gccgcttttc
    12001 acatggatga tattagtgac caggaatatc ggatctcatt gaaaaaagca gcagcaatcg
    12061 taaaaaacga aacaaaagaa cataacatat ctgctattca ttttgcagtt gaaaaagatt
    12121 tggagaatcc tacgaatgca acatatgaat accagttttt ttgtgaagga caagtcatag
    12181 cagtcaatcc atccactggg aaaacaaccg tacgaaatga cacggaaaag gccgcagaac
    12241 atctttttga tatagaaaaa acggaagagt tcaaacaacc gcaagcagcg atgaaggaag
    12301 cagtcagcta ctacgggaaa aaagcaaaag tgaaagagtg gacacttcta gtcaaagatg
    12361 aacgtcctta ttataaagtc cgattaatag atggagaaca ggaccgtcag gtatggatat
    12421 ctgcatagaa gaaggatatt gcttctcttt tttacaatat aagagtaagc taagagagta
    12481 aagatgaaag gaagttgaga taacatggaa aaaatgaaat gccctaattg cggaaaaaaa
    12541 tttgcatacg aagaagtcaa taatgtagtc gaacataatg ataaagaaat gccagtcgtc
    12601 tgcccttatt gccgaactga agcggcaaga atcgttactc atggttattt cattactgaa
    12661 aagattgaag attttttaaa ataacgattt tgcgatttct ttttctgcat gttagaatgg
    12721 agaaaaggag aggaaactat gattcgtttt gcgagaaaag aagatgctcc agaaatcgcc
    12781 ccattgatct tagtgattct aaaagatatg gaactgccat ttttgaagca gacaggggaa
    12841 caaaagactt tagagatttt agaagaagca atcactgatc caaattatcg ttacagttat
    12901 tcacgaggta tcgtagaaga aatagacggt aaagttgcag gagttgctta cggttataca
    12961 gatgaagaag aatcaatcat cgatcagcca ctggtaccta tcttagagaa atatcagcta
    13021 gatgcgtcta cacgtttgtt cattgataaa gaaacgtttc ccaacgaatg gtatttggac
    13081 tctatcagtg tatcagaaga ttttagagga caaggcatcg gttcgcgatt attagaagca
    13141 ttacctaaat tggcgaagaa agctaatcgc tccgtcatcg gtctaagtgt ggatgaaaaa
    13201 aatccaaagg ctaaaaaatt atacgaacgc catgacttca aagttgtcgg tcagcggatg
    13261 atcagtggtc atctttatga tcatatgcaa aagaatatct aaaaagatgt acctgaaatg
    13321 cgtatttccg catttcaggt acatcttttt aacttaagtt aagtcggtaa gaaacaggct
    13381 tatcgcgact tttgtcttta cggtagagcc agtaatctaa aaccgcagca atacaaatac
    13441 aaactggagc atcttcatca tttgagacat ccaatacata gtaatctcct gaaaaaagtg
    13501 ttgctttatc cattttcatg atttgctgat tgaaatgatg gatttgatat tgatgattat
    13561 agatatcacc ttggactgtc cagtgaagtc tacgaatata ataaaagtca ccgggccaat
    13621 tgaatatttt ctgcatggtt cccacctttt cgaactcttt atacagctca aaacgcgtac
    13681 cgaatgtcag gcttatctgc ttgatgtgag caacgagatc gccattcatg gcataaagag
    13741 acaatgcatc gccctttgtt ccccagcgac caaccatcaa aaacagggaa tgtccttttt
    13801 cgtttttgac aattgtccga gttgcacgtc caagctgctt ttcctgaatg aaaaattctg
    13861 acatctatat ccctcttttc tagataaaac tacaagtctt ctgcaatcgc gcgcaatatg
    13921 gcttttccta cgccagattc aaggtttgtg tccgttgtat atttggcata atcttttact
    13981 tcgatttctg catttcccat tgcgaaactg actcctgcta cttgcagcat gctgacatca
    14041 ttgaagttat cgccaatcgt catgacttct tctaaagaaa tcccacgctc gtgagcgaca
    14101 tgggcaacag caattccttt ttgagcgttt ttatggttca cttcgatatt gttttgacca
    14161 gaggaagtaa cggctagatc gcctaaaaga gaaatctgct ttccaactgg acctaataca
    14221 gctggccctt ctgtatgaaa acaaataatt tttaaaactt ccagattctt ttcttccaaa
    14281 atagcacgaa tactgtccac gtatgtgatg tgcaataact ccagcttagc tgaggccatg
    14341 gcaatcgcca ttttatacgt aaggtgaggt aaatgggtag ccaccatgga agcaaaactt
    14401 tcaattcgtt tcgcttgatt ttcagaataa agaccatcgt tggttgctac ttcatagtaa
    14461 ataccgttag catctagaat atctaaaaca gagattgctt gattatttgg aatcgacacg
    14521 gtaaacaatg aatttccttc tttatcaaat acctgtgccc cgttcaaagt gatcatcgca
    14581 caatcgattc ctacctcatc taaagctgcg cgagcttctt gatagtttcg accagtagcg
    14641 accatgaact caacaccggc atcatttgca aaacgtatcg cctcggcatt ttcaggagaa
    14701 acactcatat gggcatctaa taatgtgccg tccatatctg atgcaattaa tttgatcata
    14761 atgattatcg ttcctttctg cttcttgttc ctagtttacc atattaatca agtgctgagg
    14821 gtgaagattg tcccttgtga ttgaagaaaa tgtgagttat tctttttatt aggaggtgga
    14881 gtttttgaaa gaaatcattc ataacagttg gcaagaagtt ttgtcctcag aattttcaaa
    14941 agattattat ctgcatctaa gagaattttt aaaaaaagaa tatgcgtccc aaaagatcca
    15001 tccagatatg tatcatatct atgaagcatt ggaattgact ccttatgaag aagttaaagt
    15061 ggtgatttta ggacaagatc cttatcatgg agaaaatcaa gctcatggct tatctttttc
    15121 tgtgcaacct ggcgtaaaga tcccgccatc gttaagaaat atctataaag agctttatga
    15181 tgatcttgga atcgcacctg ttcaacatgg aaatctagtg tcatgggcta aacagggagt
    15241 ccttttactc aacaccgttc ttaccgttcg ggaagggcaa gcgtattctc atcgcgggaa
    15301 aggttgggaa agattaacag acacgatcat tgaaaaatta aatgaacggg aaaaaccaat
    15361 cgtgtttatc ttgtggggaa aaccggcaca agaaaaaatc aaaatgatcg ataagagtcg
    15421 ccatatcatc ataacatctc cacatcccag tccgttatct gcaagtcgtg gcttttttgg
    15481 ttccaaaccc ttctcgaaaa cgaatgatgc gctccttgcg ttaggagaag aaccgattga
    15541 ttggcaactg cctgaaacag tgtaataaaa cagcgctttc ctgttatagt gcagttcgct
    15601 ttgtatgtga aaaacttaat ttttccagca tttttgactg atacagataa aataaactgt
    15661 tctttttttc tggaatagtg tatgataata catataaaaa cgtctggagg tcatcttgtg
    15721 gaattgtttg atagtttaag atttaaaatt gttcgccgtg ggatcaaaat cgttttcccg
    15781 gaagctactg atgctcgtat tttaggcgca gctgcccggt tgaaagcaga agaacttgtg
    15841 gaacccattt tagtagggaa cgaagaagaa ataagaaatg cagcgcatgc cagaggaatc
    15901 aaaacatcta gctttacgat catcagccct gataattacg ataagtggga tgaaatggtg
    15961 gatacttttg ttgaacgtag aaatggaaag gcatccaaag aacaagcaga aaagatttta
    16021 aaagatgtga attactttgg tacgatgctt acgtatatgg ggattgcaga cggaatggtc
    16081 tctggagcga tccattcgac tggcgacact gttcgcccag cacttcagat catcaaaaca
    16141 aagcctggca tcagtcggac aagcggagcg atgatcatgg tacgtggaag agatcaagaa
    16201 aaatatgtct tttcggattg tgcgatcaac gtgaatccaa ctgcgcaaga attggctgaa
    16261 atcgccgtag attctgcaaa aacagcagaa ttgttcgata tcgaaccaaa agtagcaatg
    16321 atgagcttct cgacaaaagg ctcagcgaaa tcgccagaag tagacaaagt agttgaagca
    16381 actaaaatcg ctaaaaaact agcgccacaa tacatgatcg acggcgaatt gcaatttgat
    16441 gcttcttatg ttccttcagt tgcacaactg aaagcgccag attcagaagt agctggtcaa
    16501 gcaacagtct ttgtatttcc agaattgcag tcaggaaata tcggctataa aattgctcag
    16561 cgtttcggta actttgaagc aatcggacca attctacaag gcttgaacaa accggtttct
    16621 gatttgtctc gtggagcaaa tgaagaagat gtatataaat tatcgattat cacagcagca
    16681 caaacattga ttgattaaca aatacacgcg aactagccaa aaacagcagc tgctgttctt
    16741 gactagttca ttttttcttt aaaaaaactt cattacgcga tatactggca aaaagaaaga
    16801 agagcgggag aagatgaaaa atgatcgaac tatcaggatt aacaatgaca gaacaattcg
    16861 cagaggcaat tggtcattcc gcaatgccag gtgacaatct gatattgact ggtgatctag
    16921 gtgcgggtaa aacaacgttg acgaaaggaa ttgcccaagg attagggata acgcaaatga
    16981 tcaaaagtcc gacatacacg attattagag aatatagtca aggtcggatt ccgttgtatc
    17041 atatggatat ttatcgggta gcagctagcg gagcagattt agggttagat gaatattttg
    17101 aaggtgatgg gctttccgtt gttgaatggg gaaatctatt agaagaagca ctgccggagg
    17161 attatttgga actgatttta gaaaaaagtg atacagattt agaaaagcgg tatgtgaaac
    17221 taatctcata cggggagcaa agtgaaacct tcaaacagcg aattctagaa aaatgggctg
    17281 aaaatcatga gtgaagaagt tgggatcatc ttgcgggaag cagtgccagg agatgcaaaa
    17341 gacattcttt tgatgatggg acaagttaat aaggagacag aatttttagt tttggatgaa
    17401 gcagaactcc tgttaccacc agaaacctta gaagaagagt tggattatat atacgaaagc
    17461 aataataatt tattgctgct tgcaatctat gaagggacga ttattggaac agcatctgtc
    17521 aaggcagaca gtcaatttcg tttgtctcac gtgggagaag tcggtatcag tattctgcag
    17581 gaatattggg gaatgggact aggcactctt atgctagaag aaatcatcag ttgggcgaag
    17641 gaaatgggtg tactttttcg attggagctt gacgttcaag ttcgtaatga aagagccgta
    17701 cacctttacc gaaaaatggg cttccaaatc gaagcggtca tgcctagagg ggcaagaaca
    17761 gatctaggtg aatttttaga tgtatataaa atgagttatt tgattgaata gaagaaatag
    17821 gaaaagagtg agaaactgtc atccttttga gaaggcagaa aaacacacga agggactgtg
    17881 acaaaagtcg tgtcgcagtc catttttttt gaataaacgg tgttcaatca gctgaccttc
    17941 ggtattagct caaaaaacgg caaaatatga ggaactattt tgccgtttgt ttctctaata
    18001 ctcaggtcaa aacgctgatt tcacaacctc ttggtaaacg gtgtccaaaa gcttgcatct
    18061 gcggtaataa gtccaaccaa atgaaaaata tgagaagcta tttttgtttg gctgttctta
    18121 ttacttgatg cagaacagct ttttcacaac ctctccaata aacggtgttc tcctgccagc
    18181 tcctcggtca ccagtcacaa tatttaataa acatgagaag ctattttttg aaattgctcc
    18241 ttgtgactcg gagtgaaacg gcaggctcac gacctctttg tgtgtttcat aagtattctt
    18301 ttactttata gtcacaagtt ttttcaatgg atctgtcccg aatttttttt cttgataaag
    18361 caggatttcc gcacatgcac gactatcttc caatgcatca tggtgatgat ctaattggat
    18421 gttcaaattt tcgcacacag tgttcaatcg atgatttgga aattcaggaa acaggcgacg
    18481 gctacttttc actgtacata aagaaagata attaggctgt tcaagatgat aataatctaa
    18541 gcatcccgct aagacctttg tatcaaaagc agcattatgt gcaacgacta aacgattttt
    18601 ttgaaaatat ggttggatag tttgccagac ttctgggaat ttcggcgcat tgcgtacgtc
    18661 ttcttgatgg attccgtgaa tttggacatt tcgccagaaa aaaggagtct caggctgtat
    18721 caatgtatag aattcatcaa cgatctggct attttttacc atgactagcg caagagaaca
    18781 agcactataa ggctgatgat tggcagtttc aaaatccatg gcaacaaaat tcatttctgt
    18841 aaaacctcct tgggaaatta ctgtaacata cttttgaaaa aagcaaataa atttaacaaa
    18901 aaacatagaa ttttttatgt aatatttgtt gagaaaagct agtttttgaa ctcattcctt
    18961 gtgatttgga gttagatggg gatcatgact tctcggtatt aagtcaaatt tatgcaaagt
    19021 tgtgaggaac cattttcaaa aaaatttctt acgactcgaa agtaggcgac tttttcacaa
    19081 cttctccact aaacggtgtt caatcagctg accctcggta ttagctcaaa aaatagcaaa
    19141 atatgaggaa ctattttgcc atttgtttct ctaatactca ggtcaggacg ctgatttcac
    19201 aacttctcaa taaacggtgt tcaaaagctt gcatctgcgg taataagtcc aaccaaacga
    19261 aaaatatgag gaactatttt tgtttggctg ttcttattac ttgatgcagt gcagcttttt
    19321 cacaacctct taataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa
    19381 cgaaaaatat gaggaactat ttttgtttgg ctgttcttat tacttgatgc agtgcagctt
    19441 tttcacaacc tcttaataaa cggtgttcaa aagcttgtat ctgcggtaat aagcccaacc
    19501 aaacgaaaaa tatgaggaac tatttttgtt tggctgttct tattacttga tgcaggacag
    19561 ctttttcaca atctctcgat aaacagtgtt caaaagccag tttctcggta ttatgccaaa
    19621 tttatgcaaa atgtggaaag ccattttcac aaaatttttc ttaatactcg aaactaaacg
    19681 actttttcac aacctcttta tttcaaatct aattctactg gacaatgatc actgcccata
    19741 atgtccgtat ggatctttgc atcagctagt cctggctcta aatctttgct cgtcaaaaag
    19801 taatcgatcc gccaaccagc attgtttttc cttgaattga atcggtagtt ccaccaagag
    19861 tagacacctt cctgggttgg ataaaaataa cggaatgtat cgataaaccc gttatccaac
    19921 agtcgagaaa gagccgctct ttcttctgga gtgaatccag catttttttg attcgttttc
    19981 cagtttttca aatcaatctt ttgatgagcg acatttagat ccccacacac gatgactggt
    20041 ttttgttttt ttagattttc caaataatta taaaaggctt cttcccattc taatcggtaa
    20101 tctaaacgtt ttaattcgct ttgagaattt ggcgtgtaac aggttacaag aaagaaatcg
    20161 gaatattcca aagtaatcag acggccttct gtatcatgga tatcgatacc cattccgtaa
    20221 gtggcattca atgctggttt tttagcaaaa atcgctgtgc cggaatagcc ttttcgttcc
    20281 gcgtaattcc aatattgata ataacctgga agatctaaat caatttgtcc tgcttggagt
    20341 ttcgtttcct gcagacagaa aaaatcggcg tctaactcat gaaaaacttc taaaaaattt
    20401 ttatttacga tggcacgaag cccgtttacg ttccaagaga taaatttcaa taaaagcaca
    20461 ctccttcgaa ttaatttcct tacctttgcg atactattgt acctcatttt agtaaaaaat
    20521 ggtgtaagat gaaagtaact aaatgatcca ggaggaaagt agatgaagat tggtgtaatc
    20581 ggtgtaggga atatcgcaga aaaagcatat ttaccaactt atgcgaaaaa acaaggaaca
    20641 cttgattttt attttgcaac aagaaataaa gaaacaaaag aaagaattca aaaagagtac
    20701 gggttccaac atctatatga aacgatcgat gagcttttag aagaaaaaat cgaagcatgt
    20761 atgatccacg cagcaacaaa agtacattat gaattagcca aaaaatgtct agaaaatggt
    20821 atccatgttt atattgacaa accactgagt gtccatctaa atgaagttcg tgaattgcaa
    20881 gaattagctg ataagaacca aataattttg atgatcggtt tcaaccggcg ctttgctcct
    20941 atggtagaag aattgaaacg tatcccagat aagcggttga tccacctgca aaaaaatcga
    21001 atagcggcaa aagaaacgac agagttcgtc ctttatgatt tgtttttaca tcttattgat
    21061 acagctgtgt atttattaga tgaacccatt ttgagaacaa aataccagat aagagaaaca
    21121 aaggaatttt tggaatatgc cattttacaa ttagaaacac aaaatcaaac ggctatttta
    21181 acgatggatt tagcaagtgg cgctaatata gaaaaatatc aagtaacaag taaaaatggt
    21241 acttatgaag taaacgatct gacccagctt gtcattcaag aggctgatgg aaaaaaagtg
    21301 aaagaatttg gcgactggac aaacacgcta gtcaaacgcg ggtttgagcc aatggtagag
    21361 gccttctttt ctcagatccg tttaggtaaa tcggaaacag aattgcttaa acaaaaagaa
    21421 gttgtaagaa gtcatgaatt atgtgaggaa gtgcttaaag atcactaccg tcatcaactt
    21481 tgaacaagac tggcaagatt gtattaagcg tgatagaata atgaggacgt ttgagaaaag
    21541 aaaggaaaat cctcagtgaa taaagaagaa atagttgaaa gcttagccac gattactata
    21601 ttgtttgatg aaccattgat gaactatacg tttacgaaaa caggtggacc agctgatgtc
    21661 cttgcattcc cgaaaaaaca agaagaagta aagcaaatca tcgattattg ccgaatccat
    21721 gatatacctt ggatggtatt aggaaatgca agcaatctga tcgttcaaga tggcgggatt
    21781 cgcggtgtag tcatcatgct gactgaaatg aaacagattc atgtcaaagg aacgatggtc
    21841 attgcggaag caggtgcctc actgattgat acgacttatg cagctttggc agaatcattg
    21901 actggatttg aatttgcttg cggcatcccg ggaagtgtcg gtggagcggt ttatatgaat
    21961 gcaggtgcgt atggcggaga gatcaaagat gtttttgctg aagtggatct tttgcttgaa
    22021 gatggaacat tgaaaacttt gacaaaagac gaaatggtat tttcttatcg tcacagtaaa
    22081 gtccaagaat tacgtgctat cgtattggaa gctcgattca gtttgcaaac aggggattat
    22141 gaagcaatca aagcaagaat ggatgagttg accgaattgc gtcaatcaaa acagccatta
    22201 gaataccctt catgcggaag tgtctttaag cgcccagtag gacactatac aggtcagctg
    22261 atccaacaag caggattgca aggcctgaaa tggggcggtg cacaagtctc tgaaaaacat
    22321 gctggattca tcgtcaatat cgatcatgcg acagcgacag attatattga attgattgcg
    22381 cacatccaag aagtaatcaa agaaaaattt gatgtgtctt tagaaacaga agtgcggatc
    22441 attggagaaa aacaagcaat atcagaaaca agcagaagtt tagccaatta ataagtaatg
    22501 gaacttaaaa taagtaaaag agcaggacgg tggccgtcct gctctttttt gtggttgttt
    22561 acgcaaatgg cgcaaatctt gtacatttta acacaataaa acaaaaaata tagaatattt
    22621 cctcataaat ttttccattt tttgcaatat tctcaaaaaa aagataaaaa atgagaataa
    22681 ctgatgaaaa aacaagcata atccatcata aaatcagtat aggtgaaaag atgaactttc
    22741 atctgcatat caagtaattg gtatgaggca ttttttgagg tgtaggatat gaagttggat
    22801 taccttatag aagaggaaga tagagaaaaa ctgctgctag ctagatattt agaatttgag
    22861 aaaaagaatt atgtagtgct gcctaagtta tatgaaacat taggtttgtc taaatttaaa
    22921 acaagaaatt atatcataga attaaataaa gaatttactg aattcaaaac aaagccagag
    22981 atccaaattt cagaagaagc agagatccat gtcgaacgtc tgaatttgac ggatatcaaa
    23041 aatttaagaa gaatctactt tcaacgatca aaaattgctc gactgttaac ggagatcgtg
    23101 gattcaaaag taacgatcga aaaatttgca gagaatcatt ttttaagcat atcaagagcc
    23161 tatgtaaaaa gaagagaatt agtctctttt cttgaacagc atcaaataaa gttaaagaaa
    23221 aacatattag ttggggaaga aataaacata cggaacgtat tatttggggt atatttcggg
    23281 gtctttaatg gtttttctat gccatttaga gaaagcgtca tgaatgaagc aaagagtatc
    23341 gtgcaatatt ttgaatatct gttcaattta aaactgtctg caacaaaacg aatcaaattc
    23401 aatttgctaa ccactattag tttgttgagg gcctatggga atcaaccaat cggaaaacgt
    23461 ttcttttcag atgaatactt taagagctca gaaggaatga agatcatcaa ggatttatca
    23521 catatttctt ttcttcatat ccctaaagag cagatcattg atgagatttc ttatatcatg
    23581 ttgtttttga tcacggaaga agaatttgaa ttagatggcg catttggact tgcggaagct
    23641 cattttcatg atatccacat gattagtcaa aaaatgtcag tggaattgat tgatcaatta
    23701 ccattttcga ttgaaatctc tgaagaagaa agactatata tacgaaaaga acttcgtatg
    23761 ggtatgatgc atgtcaatcg aaaaaattgg atattcgact ttgagatgac tagctttacg
    23821 tctaaaaagc agctacagtt tttcttcgag acgtatccaa tattcagtac agcaatccaa
    23881 aaggtcatac aagagaataa acgctatttc ttgggaaaag atgaacgttt catgattcgc
    23941 ttattttatg attatctctt tttattggtc ggtatttgtc cggtatcata ttttgaagca
    24001 ccgattcatg tctgtattga tttttcgcaa ggatctgctt ataccgatta cattatttct
    24061 caagtgaaag gatttaaaaa ttacaatatt gtgattgaga gtcgaatcac ttctcgtacg
    24121 aatatttatt tatctgattg tttacttgaa aaatttaata aagaacagat aatttggaaa
    24181 aatccaccga ctccagatga ctgggaatat tttggaaata ccatcatcag agaaaaaaat
    24241 agacatttaa ctgaaaaaat cagctagtat ttatggatgg cagctttgtc tcacatattt
    24301 attagctgat ttatgttttt ttcaaagaaa aggtaactag atataactag atataactag
    24361 atatattact taacaaaatt aaaaagtaca taagttatta ttccgatttt cttgaaacaa
    24421 taaaaaatca aaaaaaatac tctcctaata gtccatcttc ttttgatttt atttatataa
    24481 tgaaatcatc ttatgaaaat tactacttca tgtttagaca agagtaggga ggactaagaa
    24541 taaatgtgtt caaaaagacg ttcaaaacaa ctaaataaac acgcttgtcg tttagataag
    24601 gaacggtcgt gttctgaaaa acactacgtt aagtttatga tgcgcacgat gctgatcagt
    24661 ttatctgtag gtgtctatat gcatgtaccc gttcaggcaa cgacacaaac catcaaggtc
    24721 gtacgtacct ctgtttctga gaccactgat tcccctatca ctattactca tactttcact
    24781 gatgcaaatg gaaaacagct acttcctgca ttgacttcct ataataaagt cagttatcct
    24841 ccaacgataa accactactg gttaattcct aacaaaacat tatggacatc ggaaaccgga
    24901 agcagtgtgc cgctttctat cttttccgat atgactgatc ctacaaaagg tgatcagtta
    24961 aaacagatca ctgatatttt gaatcatgct ttgacagata taaaggacgg tggaagcgca
    25021 caagtttctt atgtatatga aaaagattat tcacagtttg aaagaaagaa aatcgatgtt
    25081 ctggcaggga caaaagtttc gatatctgat ctggtagaaa ggctgaagaa cgcagatgga
    25141 acacaaggag atatgtccaa agtaatttca aacaatggga atatcgtgga tacgactaat
    25201 acaggaacat ctactatttc cttgtcctat tttgatccaa ttgctttaaa aacaatgact
    25261 gcatctgctg tgatccatgt tcgtgctgat caaacagcta tcaaaggaaa agatcttcac
    25321 ttaagaacag gagagagtta cgcccctaaa gatcttgttg aatatgtaac aaaaagcgat
    25381 ggaacttcag gaaataaaga agaaatcaaa atgaacggga cagtagatac tacaactccg
    25441 ggagaatatc ccgttgtatt aacttatata gactcgaaaa catcaaatgt ggcagaaaca
    25501 acagctgtag tttatgtaga agcatctgct gcttcatcga ctgtcatcac tcattttata
    25561 gatagtcaaa caaataaaga acttcaagca gaaacacata tgagtagtgt aaatcgtatt
    25621 cctgatttgc gcctacctgc tgaattttct gatcgagtgc caaatataga tttatcgact
    25681 tatgaacaaa atgggaaagt cacttctatt tctgattgga tgcaagcaaa cggattagac
    25741 agaaaaagtc actggtcgga gatccttgaa gtacttcact cagaactgat gaataatagg
    25801 aagcaatcag gaggggaaat tacctttact tatgtttata taccagacaa tcgttcatta
    25861 gaaggtcatg atcttattgt tcatccaaac gaaaaaataa acaaagaaga gttgatccaa
    25921 tcagcaacaa attccttagg taaacccgta ccgttaaagg atgtatcggt tacaatcaat
    25981 gaagtaccat tggaggatgg ttatttgacc aaacaaacag gtgattttac gattatgtat
    26041 agttatcatg acccgtattc actccaagaa ctccatgctc agtcactatt acatgtttta
    26101 ccattaaaca aacagataga tcaaaaagaa caaaaaagac gagaagaaag cttgtcagaa
    26161 aaaaaacaac aaatggctca tgcagtttct ccaacaggga aagtaaaaca aacaagaagc
    26221 ctagagttag caagaatatc tagaaaaatc tcagaatcta ataaaacgat aaggcaagaa
    26281 gctcatttgg ccccacaagt ttctggattg gaaaatgaaa ttttagagcc taaaataaaa
    26341 caacaatcag aaaaagaatt tgttgctaaa cagtcggaag atcagaagga atcagatgac
    26401 cagaaggaaa cgaagaaaaa acgcttgtca actattgatt ccaccgatga gcatctgcag
    26461 ccgcaagtac cacttcctag tccgggaaca cccgcacctg gtggaggtat tccttcttct
    26521 gctttaggag atttcatgcg aggtatggca ggaacatggg tatacgctaa tcgaaatgaa
    26581 tttgacgcag atgactggtt ctgatttata tctctatctg ttatagtcaa ataagtagct
    26641 gagtcatatg ttatttggca taatggagac gaggagggat agaaaaatgt tagataaaac
    26701 gttaccttac gcagaaatct ggatgacaag accacttcaa caaaatgttc caatcacgcc
    26761 tttagcggaa ggttttgaat ttcgatttta tcaaaaagaa gatgaatctg cttgggcgga
    26821 gattgagact tccgttgggg aatttcaaac gacagatgaa gcacttgagt attttcaaca
    26881 aaactttgct ccttatcctg aagaacttga aagaaggatg ttttttgtag agacaaaaaa
    26941 aggagaaaaa gtagcaacct gtaccgcttg gcgaaaaaaa tcaagagagc aagtgtatcc
    27001 ggtttttcat tggttagcag tgaaaccaga ttaccaagga aaagggctcg caaaagcatt
    27061 gactgccaga gtcttgcaag aatttcctac cctccatacg gacggaccaa tctatcttca
    27121 cacacagaca tggagccatc aggcgattgc tctttataaa aaaatgggtt ttacttttat
    27181 tgctgaaaat ctagacggca cacctaatcc agattatgaa aaagttatgc aggttttaag
    27241 taaattagat aaataacgaa atggtgaaat aagtcctcag tattgggata aaaaacgaaa
    27301 tagtcattcc tcatatctta cttttttgag ttaatactat cggataacta atggcatgcc
    27361 atacattcaa aaaagaaggg gcagtgacaa aagtattgtc actgcccctt ctcttgtttt
    27421 ttagcatgtt ttatcgtttg atttttttag gaataaaatt cttgatgaac gtaaattttt
    27481 ggtaatcgtt tcgactatag aggtaaccaa atacactgaa aacaactgct ccaataaaat
    27541 taacaaataa atccttcata gtatccatga ttcccacgtc taagtaacca ttttggatga
    27601 ttgttttatc tgttttacca tctgcatttt gactttcaat gattgtccga gtgatatctg
    27661 gaacatgata gacagtgttt ccgtctttac ttaactccac actattgatt tgctggatga
    27721 tcgtatcttt ttgcatatcg aattgcagta atttatctgc tgaaaattcc acaaactccc
    27781 acataacacc aactgtcatc gaaaaacaga aggaaacaac tgctagtaat aaaggagtca
    27841 agttcatggt ttttgtattt ttattgagca aataaacaag ggaaaagccg acacccccag
    27901 ctaaaaatcc gctgaatgca tgtagaaaag tatcccacat tgaaaaatag ccataaaaat
    27961 cacttaactc tcctaaaact gttgaactaa aaataaaaga taacaaaatg atttctagca
    28021 tatttggaat ctgtatcttt aatatctttt ccagaaaata agggatttca aataaaatta
    28081 atgtcagtaa taaaagcatt gtatttcccc agtgttggtt ccagacctga tccactagca
    28141 taataacgac gatgatacgt aataaaatat agattccgtt tgtgattcta cctgcagttt
    28201 ttgtactgtt cgtaaaaaag gttttcaaat gattcataaa ataactcctt catgtatatc
    28261 cttaattata ctaagaagcg tgtgtttacc aaagctatat gacttaaaga aaaacagagt
    28321 tgatattgtg aaaaaaatca cttaatatag tataaggaga aaagggggat acaaatgaaa
    28381 aaattagttg ctggtttatt aacagccgtt gcacttcttg ctatgagcgc atgcggcgca
    28441 gatcagaaaa atacagcgca gacaagttct agtacgaagg aatctgccga agtatcatca
    28501 ggcgcttctg aacaagtata tacagatcca aaagaaatga aagaatctta tgatgtcgtg
    28561 attattggag ctggtggtgc agggatggca gcagctatcg aagcaaaaga tgctgggttg
    28621 aatccagtaa ttttggaaaa gatgcctgtt gccggaggaa acacatctaa atcatcaagc
    28681 ggtatgaatg cttcagaaac aaaatttcaa aaagaagaag gaatcaaaga ctcgaatgat
    28741 gccttctatg aagaaacgct aaaaggtggt ggaggaacga atgataaaga aatgcttcga
    28801 tactttgtcg atcactctgc tgatgcggtc gattggctag atcaaaacgg tatcaaactg
    28861 gacaacttaa cgatcactgg agggatgagt gaaaagagga cacatcgtcc gtctgatggc
    28921 tcggctattg gtggatactt agtggagggg ttattgaata acgtccatga gcgggaaatc
    28981 cctatttttg tcaacgcaga tgtgacagac attaagaaaa atggggaaac ggtcaatgaa
    29041 gtgactgtac atgttcaagg agaagaacca aaagaggtca ccggaaaagc aatcgtagtt
    29101 accacaggcg gttttggtgc cagcaaagaa tttattgaaa aatatcgtcc cgatttagct
    29161 gattatatta cgacaaatca agaaggatca actggagatg gtatcaccat gattgaaaaa
    29221 ttaggtggac aaaccgttga tatggataaa atacaaattc atccaactgt gcagcaggat
    29281 gagggggtct tgattggtga ggttgttcgc ggagaaggag caatcttggt cgatcaagag
    29341 gggaaaagat ttgtcaatga aatggataca agggataaag tatctgcagc aattacagca
    29401 ttgcctgaaa aatcagctta tttgattttt gaccaaggcg tgcgtgatcg tgccacagct
    29461 attgaatttt atgaccaaaa aggatatgtc acaactggaa agacaatgga agaattagca
    29521 gaaaaaatcg atttaccaaa agaaacacta gcaaaaacag ttgatggatg gaatacggat
    29581 gttgcggaga aaaaagatgc tcaattcaac cgtgccacag ccatggaaca tgacttatcg
    29641 tctccgaatt attatgcgat caaaatcgca ccaggaatcc attacacgat gggcggtgtg
    29701 aaaatcaata cgaacactga agtattagat aaagaaaacc agccaattag ggggttatat
    29761 gctgctggtg aagtcacagg tggcttacat ggaaacaacc ggatcggtgg aaattcagtt
    29821 gcggagatca ttatttttgg gcgacaagcg ggcatacaag cagctcgatt tgcttcggaa
    29881 aatgaatgaa atagaacgta aataaaaaga ggttgtgaaa tcagcgtttt gagctaatac
    29941 cgagggtcag ctgattgaac actgtttagt gaagaggttg tgaaaaagct gccctgcatc
    30001 aagtaataag aacagccaag taaaaacagc ttcttatgtt tttaacttgg ttggacttat
    30061 taccgcagat gcaagctttt gaacaccgtt tattcggaaa aagagcgtgg gacaaaagta
    30121 aagaacactt ttgtctcacg ctctaaacac gtataaacgg cggaagcaga agaaattcct
    30181 tcggaaataa gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat ttcttcttat
    30241 ttcttggagt taaacgcttc tgtcccagcc tcttttttga tcagcatgat tttattcagt
    30301 tatcttcaaa atgatttttc cgatgttttt gtttgcttcc atatattcat gcgcttgttt
    30361 gactgcgctg aattggaatg tacggtcaat gattggccga atcttgccat tttctagaaa
    30421 aggcccagct ttttgcataa aagtttgtgc gagctctgct ttatactgat cacttctagg
    30481 tgtcaataat gtaccaagca gttggacacg tttcccaatc aatacaccaa ggtctatctc
    30541 aggcacgatg gtcccaccta gcataccaat caagacccaa cgtccatcaa cggctataga
    30601 agctaaattc ttttcccagt aagaagcgcc gatgaaatcc aaaataacat cgactccccg
    30661 accttcagtg atttttcgta cctcttcttg aaagtcttgt tttttatagt tgatcaattc
    30721 atctgcacca agttctttac aaaaggcgag tttttcttct gatcctgcag taacgatgat
    30781 tttcgcgtga gaaagctgtc tagctaattg gatcgctgct gttcccacac cacttgctcc
    30841 tgcatggatc aagacagttt cgccttcttg taattttcca agccagtaaa gtgtttgata
    30901 agcagtcaaa aaaacttctg agattccagc agcagaaaca tagtctagcg tatcagggag
    30961 aaggattgca cgatcggcag gcatcacagc atattcggca taaccaccta gattgactaa
    31021 tccgtatact tttgtcccag gagtaaaagt ggagatatca cttttattct caactacctc
    31081 tccagctact tcgactccca aaatagggta gggaggtttc aaagagactg attctctttt
    31141 cataatatct gttcgattga cagctgctgt atggactttg atcagtaatt cgccttgttt
    31201 tggtttagga acaggcactt caatttgttc taatacatct acagaaccag gatgcttgat
    31261 tgaaatggca cgcatggatc tattcctcct ttgcccattc ttggaatgct gtattcaact
    31321 ctttgctacc ttcagaaagt actagagtcg tacgagaaat atccgtttct tccactagag
    31381 cggcaagtac agcagcaact gtagagcgag tgatcgtttt tgatgccttc tcattaggct
    31441 tagttaattg aatggaagta acttcttcat cgttagtcaa tgtgactggt tgaagaatga
    31501 caaaatctaa gttagttcgt ttcagccatt catctgcata atgtttagta atataataat
    31561 ctgtcatgga ctcaggccat tttgctggtt catctgcata aacagcactg accatcagat
    31621 agcgagagac atgcgcattt tcagctgcga tcactgtttt gattgcgcca tccaagtcta
    31681 cttgcagaag attctttcct tgagagccgg cagcaaaaat caatacatcg atgcctttga
    31741 atgcttcggt cattttttcc acggaccaag ttaaatcaaa agagacatac gaaacattct
    31801 ctttttttac taatgtttgg gaagcaacat ctctcacccc tgcaaaaatt tcatgggaac
    31861 ttggagctaa atcttttatc aggtgtcggc caatttgtcc gtttgcacct acaacaaaaa
    31921 ttttcatttg gatcatcctt tcgacgtcaa atctttccat tcaatagtaa caccaaaaga
    31981 gaaaaaaatc agagatcggc tacgatgact atcagttagt aagagaaggg tgcttgaagt
    32041 atcttaacaa gacattgcat actgctgcag tcgcaagacc tgcgacgatt ccttcaggta
    32101 taccacttgt aaaaataacc gctagaatag ctgaataaac agcatttaaa tttgttccga
    32161 tgactttggc ataatcttgc tggaataaaa agtagatcat attcatcact aaaatcgtat
    32221 tggtcaatga accggaaagt ccagctaaga ataaagaaac ggaatctgct ttttgtttca
    32281 ttagtttgcg cagaccttta taaacgaaat aaggaaccac cccaatcaag atgcgaggaa
    32341 taaacgcgat cagcaaggct ttccagcttc catgttctgt tccgatgact gggataaacg
    32401 gagaaaagac aaaagacaaa ggtgtttgga taactgtgct acggatcatg ctgatcagtc
    32461 caaatgtacc gccaagaaaa gcacctagtc ttggtcctaa aacgatcgaa gcaatgatta
    32521 caggaatatg catggttgtt gcattgattg gaccgatagg gataaaacct aaaggcgtaa
    32581 cagctaataa aatcaaaata gctaaaaaca tcgcagttaa tgtaaagttt tttgtatttt
    32641 tcataagtac tccttttttc taagcatttc tttcattaag agtcagcatg acctgttcga
    32701 tgatcgtctg aatatcagct aaggcacctt ttccaaaatc tccacaagct aaaagagatt
    32761 ctcttggttc aatttcctga tagccgacag atctcaatgt ttccagatta cgctgagtga
    32821 tcgggttttg atacatattc gtgttcatgg caggggcaat caattttggt gttcccactg
    32881 gaagcgccat tgcgactgtt gaaagaagat cgtctgcaat gccgtttgct agcttgccga
    32941 taatgtttgc tgtagcaggc gcgatgagaa acaagtcagc ttgttttgct agttcgatat
    33001 gattgattac ctgcggattt gattcttgca tgacgtctgt atggactgcg cgcttcgaca
    33061 gagattgcaa ggttaatggc gtgataaaat gtgtgctgct tttagacata atcacatcta
    33121 cctgggcacc taatttgacg aattgacttg taagatcagc gcttttgtaa gcagaaatac
    33181 ttccagtaac gcctagtaag atctttttca ttttgattcc tccagtctaa ttttctcagt
    33241 aatcaatgca gcaatctcag atttactttg agcttcttcc actgttccat ctttagataa
    33301 gagatagccg tggtgtgtct cgccatggat ttccgtcaaa tcatttgcga aaataaaatc
    33361 agttttgtta tttatcagac tattttttgc gactgccaga agagattcct ttggtacatc
    33421 tactaatagt ttgaaaccaa tgatttttgc ttttggctgc cattcacgta gataagaaat
    33481 gatttttggt gttttcttca aaacgagcac aagatgctcg gtgttagaag agatctttga
    33541 aacagtggta tctgtctgtt cattttccgc aaaccaattg tccagttctt cttgcgttga
    33601 agaagtgggt aggttttttg ccagctgttc ttctgaaaaa ctaaaagcag gagtaaaatc
    33661 actgactgcc atactgtgga taatcgcatc gtagtgttct tttttcgtta gttcttctaa
    33721 ttgtaaaaat aagtccagtg cagattcgat cgtataaatc gttatattgg agtgtctttt
    33781 cggtttcagt gcttgtttcg tagtcacgta atcgattgtg gtatccggat tggcagctaa
    33841 ataatcagct aatgcttgcc ctaatcgtcc tgtcgaatga tttgtaatag cacgtacttg
    33901 atcgattttt tcagtcgttc ctccagctgt gattagaatc ttcatttatg gtcacctaca
    33961 tttactagtg ttcttccttg gtgtgttcca gcaagtagtt tagatgctgt ttcaggcaat
    34021 tgatccagag taatctcttg gacgaccagt tcatctgcaa tctgcaaatc cgccatctgt
    34081 tgccataaag aaagacgttg atctattggt acattgacag aatcaattcc aatcaactgg
    34141 atgtttcgca gaataaaagg caagacagtc gcgtttagtt tgattccccc agcattgcca
    34201 cataaagcta ctgcaccgtt ataagagatc aatggaagaa gactgcttag ctgctcgcca
    34261 cctactgtgt cgatgacata gtcgatttgc tgttttccta aagcttttgt tgtctctggc
    34321 agaaattcat caggtcgtac gacttgcaaa gcacctagac tttttagcca tgtgacagac
    34381 tcttcttttc tggaaaaggc aatgattgac tggaatccta gtttatgtaa cagggctatc
    34441 gctgtactgc ctacaccgcc agaagcacca gtaacgacaa cttttttatc ggaggtcata
    34501 ccttgacgga ttaaagcgtt cacacacaac atagcagtaa accctgctgt accaaggatc
    34561 atcgcttgac gcagcgtcat attttttggt agaggaacta accagtctcc tggtactttt
    34621 tgatattggc tataaccgcc tggatgggtg acgccaagcc caaaaccagt agcaagtaca
    34681 agatcttctt ttgaaaaacg tggattgtta ctttggacga cagttccgga taaatcaatc
    34741 cctggagtca tcggatatgt tcgtatcacc tgaccagact cgctcattgc caatgcatct
    34801 ttataattga catcagaata ggcaattttt accaagactt cattttcagc caactcagta
    34861 aatggcagat cactgattgc tgtatggaaa acaggttcct ttattacctg aaaagatcga
    34921 aaagcgttca ttcgtgagac actcctttta aaattttctt ttatcatacc ataaagccta
    34981 ctcttttatt cttgttcttc cgtaaattta gacaaaaatt aggtgaaaaa taaagaatca
    35041 aacaactaga atttattcca aaagataaag aaatattcag caaaaaaaat tttacaaagg
    35101 gttgcctttt tcaataaatt tcgtatacta atacgagtgg tagtaaacag gaagtttact
    35161 attagaaaac ttcgattcgg aggaaattat ggaaattggt gagaagttaa gaaatttgcg
    35221 catccaaaag aatttgacac aagaagaact aggcgaacgc acagatttgt caaaagggta
    35281 tatttcccag ttggaacgag atttaagttc accatcaatg gaaacattct tttctattct
    35341 tgaagtctta ggtgtgactc cagaagaatt ttttcatcaa gaagcagcga atctgcaagt
    35401 ggtttattca aaagaagatc atacggttta tttcgatgaa gaaaacgggt atgaattgga
    35461 atggctcgtg gctgattcga atgaaaaaga aatggagcct gtcttgctga cttttgaagg
    35521 tgcaggagaa tataagacgt ttgaaccttc attatctgaa acgtttattt ttgtattaga
    35581 aggggaatta gagttaagct taggagagac ggtctatgtg gcaaaagcag gacagtcgat
    35641 ctattatcaa gcgacaaaac aacatcaatt aaggaatcat ctcaaaaaac gaacaaaagt
    35701 attgattgtt gcaacgcaat catatttgta atataggggg taaaaacgtg gggaaaccaa
    35761 tcatttcatt tgatcaagta gtgaaacgct atgatgatga aacagtatta aaaaaagtaa
    35821 gttttgagat cgaacaagga aaattttata cactgcttgg tccttccggc tgtggtaaaa
    35881 caacgatcct acgtattata gctggattca ccgaagctac agaaggggac atctattttg
    35941 aagggaaacg gttgaatgat ttgccagcta ataagcgaca agtaaataca gtcttccaag
    36001 attatgcact ttttcctcat atgaatgttt ttgataacgt tgcttttgga ttgaagatca
    36061 agaaaatgtc caaatcagat atcgaaaaga aggtaaaaga tgctttgcgt atggtccagc
    36121 ttcctggata cgaaaaacgg gagattagtg aaatgtctgg tggtcaaaga cagcgtgtag
    36181 cgattgcacg agcaattgta aatgaaccga aagtattgct attagatgaa ccgttatcgg
    36241 cgcttgattt gaaactacgt acagatatgc agtatgaact gcgggatctg caacaacgat
    36301 taggaatcac gtttattttt gttactcatg accaagaaga agcgttggca atgagtgatg
    36361 agatttttgt catgaataaa gggaaaatcg tccaaagtgg tacacccgta gatatttatg
    36421 atgagccaat caatcatttc gttgctgatt ttgttgggga gagcaatatc gtggacggtg
    36481 taatgatcga agataatcta gttgagtttg ttggtaagca atttgaatgt gtcgatggcg
    36541 ggatgcgtct aaatgaacca gttgaagttg ttttacgccc agaggatttg acgatcacga
    36601 caccagataa aggaaaacta gtcgtaacgg tcgatactca gctgtttcgt ggtgtccatt
    36661 atgaaatcat ctgttatgat gaacagcaaa atgaatggat ggtccattca acgaaaaaag
    36721 ctaaagaagg aagcaaagta ggattggcct ttgagccaga agatatccat gtcatgcgtt
    36781 tcaacgagtc tgaagaagaa ttcgatgcac gtcttgacag ctacgaagaa taggggggac
    36841 aagaatgaaa acaatgcgca ggatctattc cgtcccttat attttgtggc tcggtttatt
    36901 cgtgatcgcg cctgtattaa tgatcattta tcaatctttt tttgatatga atgggcaatt
    36961 cacgctgaat aattatctat cttattttac gtcggggaca tcgctttcta tgacgatcaa
    37021 ttctgtatgg tatgcatttt tgatcacgtt tttcacgctg atcatcagtt acccgactgc
    37081 ttatttttta acaaaactga agcacaagca attatggctg atgctgatta ttttgcctac
    37141 atgggtgaat ctgctgctta aagcttacgc atttattggg attttcagtg tgcacggcag
    37201 cgtgaatcat ttctttgatt ttatcgggat cgggccgaaa cagatattat tcacagactt
    37261 cagttttctg ttcgtggcaa cgtatatcga gattccattt atgattatgc caattttcaa
    37321 tgcattggaa gagatgaatc cttctttgat cagtgctagt agagatttag gcgcgaatag
    37381 ctttgagacc ttccgacgag tgatttttcc gttattatta aatggtgtga aaagtggtgt
    37441 ccaagcagta tttattcctt ctctttcttt attcatgctg actcgtctga ttggcggaaa
    37501 tcgggtgatc actttaggaa cggctatcga acagcatttc ttggttacac aaaactgggg
    37561 gatgggttct acaatcggtg tgattttaat cattgctatg ttcatcgtga tgctcctgac
    37621 aggcgagaag aaaaaaggag gtcgctcata aatgaaaaaa tttaaatggt cgtatcttta
    37681 tttagggatc gtctttttat tcttatatgc accgatcttc tatctgattt tttattcctt
    37741 caatgaagga ggaacgatga acagctttac aggatttact tgggaaaatt atgaagctgt
    37801 ttttgaagat acacgtttga tcattatcgt attgaatacg ttcatgctgg ctttcttatc
    37861 tgcattgatc gccacgatca tcgggacatt tggggctatg ggaatctatt acacgaaaaa
    37921 acgacgtgca cggacagcgt tgttgagcat gaataacatc ttattagtct ctcctgatgt
    37981 tattatcggt gctagtttct tgattttctt tacactacta ggattcggat taggttttca
    38041 gagtgtattg ctttcccata ttgctttcag cattccaatc gtcgtcttga tggtcttgcc
    38101 aaaattgcag gaaatgaacg attcaatggt cgatgcggct agagaccttg gtgctaataa
    38161 tttccaagta gtcaaaaata tcattttgcc ttatctttct ccaggaatca tagctggata
    38221 ttttatggca tttacctatt ctcttgatga ttttgcggtt acatttttcg tcacaggaaa
    38281 tggctttacg acgctctcag tagagatcta ttctcgtgca agacaaggaa tcagtttaga
    38341 aatcaatgcg ctaagtgcgt tagttttctt gttctccatg atattggtca ttggatacta
    38401 ctttattagt aaagaaaacc gtccaaaacg gctgaagaaa aacagaagag tacgcgaggg
    38461 ggtggcacgg ctgcgatgaa aaaattacaa tctctgtttt taggtattct catcattatt
    38521 gcggtgctgc tgcttggtgt tcgtcaactt gaaaaaacaa gtggcatgag tggagcaaag
    38581 gtacttacca tctataactg gggagattat atcgatccta gtttgatcac gaaatttgaa
    38641 aaagaatatg gatacaaagt gaattatgaa acatttgatt ctaatgaagc gatgttcaca
    38701 aaaatccagc aaggtgggac aaactacgac atcgctatac caagtgaata tatgatccaa
    38761 aaaatgatca aagaaaaact agtacttcca ttagatcatt cgaagatcaa aggattaaat
    38821 aatattgatc cacgattttt agacttggat tttgatccag ataatcagta ttcgattcca
    38881 tatttttggg gaactttagg gatcgtatat aacgataaat tctttgatgc aaatcaaatt
    38941 aagcattggg atgatttgtg gaagccggaa ttaaaagaca gtttgatgct gattgacggt
    39001 gcacgagaag ttatggggct ttctttgaat agtttaggct attctttgaa tagtaagaat
    39061 atggcacagt tgacagaagc agcaaataaa ttgaatcatt taacaccaaa tgtcaaagca
    39121 atcgtggcag atgagatcaa aatgtatatg atcaatgaag aagcctccgt ggcagtgaca
    39181 ttttctggcg aagctgccga tatgatgtgg gaaaatgaac atttacatta tgtgatccca
    39241 ccagaaggat ccaatctttg gtttgataat atcgttatgc caaaaaccgt gaaaaataaa
    39301 gaaggtgcct atgatttcat caactttatg ctagaaccgg aaaatgcggc acaaaatgca
    39361 gagtatattg gttattctac accaaatgaa gaagcaaaag cgttattgcc aaaagacatt
    39421 tcgtcggacg aacaattcta tccatctgat gacacgatct cccatcttga agtctacaaa
    39481 gatctaggat cgaaatacct aggcatctat aatgacctat tcttagaatt caagatgtat
    39541 aggagataat ggcaaaactc agtaagaatg gggacttatc aacgttctaa agagctaaaa
    39601 atcgtccttt ttctatctga gttttccaat cttctaccaa aagcggatga aacgccgctt
    39661 tttttgtgtt attttatttt ttctaccacg tttttctacc agagaaaagt ggacaaaacc
    39721 attgtaaaat agggcccgag acgggctagt aaatctaaga ttgaaaaagt tacagaagga
    39781 taaagtaatt ggaaactatg caaatatact taacccttct gtaatctatt aataaaacta
    39841 atatatgaat ccagttcaat atttgtagct atgaaaaaac ataaaaatca tattagataa
    39901 aaactaaatt ggtagataaa ttattttgtc agatatctga ggcaaatatt cgactatttg
    39961 tgtttcaaag taaaaattgg aatacaaata tagaatagac atattgcagc cgatagttta
    40021 ttatgctttt agacacatta aactggagga aaaaatgaaa aagagcgcta aagatcgagt
    40081 ttttgatgag ctgatacgac taacagggaa tatgtcttct gtcactgccg agaaaatttc
    40141 gaagaatcta ggtatctctc gtcagaatgc cagccattat ttgacgagat tagtggaaga
    40201 taaaaaggtc gagaaattac cgggaaagcc tgttttgtgg aagcctttag atgaatatgc
    40261 ggttgtagat aatacggagc aaataaacga agcctttcat tctgtagttg gtcatgacgg
    40321 aagtctgaga gaggtcattc aaaaatgtat agccgctgtc aaatatccgc ctaacggact
    40381 cagtgtatta ataaatggag ccacgggtgt tggaaaaagt tttttagcca caaaaatttt
    40441 tgaatacgct atccatgagc aaattattga aaaagatgca ccatttgcta tcttaaactg
    40501 tgccgattat gcggacaatc ccgaactgct ttcggcaaca ctgtttggat acaagaaggg
    40561 ggcctttacg ggggctgaaa aggataccga tggtttattg gcaacggcca acaacggcta
    40621 tctttttctc gatgaggtcc atcgcctatc gaaagaaaat caagaaaagc tctttctatt
    40681 tattgatact ggaaactatc gtccagtcgg tgaaaatgta aattggcatt ctgctaaggt
    40741 tcgttttatt ttcgccacga cggaaaaagg tgaaaattat ttattagaca cctttgatcg
    40801 gcgtattcaa attagtgtta tgttgccaac ttttgaagat cgccccattc gagaacgatt
    40861 agagctgatt caattatttt ttcaaaatga agcggatgtt ttacaaaaag atattattgt
    40921 ttcaagagaa gccttgtctt taatgttatc tcatcccttt agtgggaata ttggtaagct
    40981 gaagaatatc attaaaatca gttgtgcgga tgtgtatagt cgaacacaag aagaaccatt
    41041 aacgattggg aaaaatgaga tcttgattca gttaaatatg ctggaatccg aagtagaaag
    41101 cttgccgatt gctagtttat tgataaagaa aaatcaaaag ataaaaacgg atcgaatcaa
    41161 accgtttgat tatgattcga ttttggaaaa aattactcag ctgataaatg aaggtaattt
    41221 caatgaaaat ttttcttcga taaagaatga agtacagcat ttggtgcatc acattgataa
    41281 ggattctttt ttatcaatga gtagaaaatt gatgattcag atgtttagaa aggtctgggt
    41341 atccgtttta ggaaaaacgt acggattgac gacgagtgga cctgttgcag aaattattag
    41401 cagatcctat gtcatttccc caagctataa gacagactta accgggataa atcgtacatt
    41461 agctgcaaat ctacctcgga cgacttactt atgtaatcag ttcttggagc atttaccacc
    41521 gctctcagaa aaggacgaga actttctaag actgcttatt gtcgtgactt tatctgatta
    41581 tgtagatgaa tccatcgagc tgaagggcct gttgcttgct cacggagata gtacggctag
    41641 cagcattcaa gcagtagtga atcaattatg cggcaattat gtttttgaag cgttggatat
    41701 gccagtagag agtaatgtgt cagagattgt cgaatcgaca aggaatttcg tgaaacggca
    41761 agtgaataca gatagtctag tgctgatcgt agatatgggc tctttaaatc aaatttacac
    41821 acagatcaag aacgatttaa aaggggagtt acttcttctg aataatttga cgacttccat
    41881 tgcacttgat attggcttga agatgacgat gaatgcgcca tttaaacaga ttgcggagga
    41941 tgcagcagac aagtattcaa tcaatgttca atactttgaa ggcttttctc aaagtagcaa
    42001 cattatcatt tcctgtatgt cgggtttggg gatttcggac aaattgaaag atgtttttcg
    42061 tcatcattta tcggatgaat ccatcgaagt tttcacgaag gactacagag aactacgtga
    42121 attaatcgac caaaatgatg aaacttattt tgacaagaca aaattggtca tcacgacttc
    42181 ggatcttcca cgttcctttt caattcctaa tattaatgtt tatgacatct tagacggtga
    42241 tggagcagcc aatttggagc atattttgag tcctgaaatg aagccggcag aatttaaccg
    42301 cttgattcag gagctagtaa aattcttttc gttggaagga attgcggacc gtttgaactt
    42361 tctgaaccca acgatcgtga tgagtgaagt ggaaacagtc atttctaaat atgaaaatta
    42421 ctatcatttc actttaaatg gccgtgtaaa actaaatctc tatatgcata cggcattgat
    42481 gatggaacgt ttattcttgg ctcggacaga aaaatctgaa gaaccgaatt atgatttgaa
    42541 accagatgaa gatgaatttt attatgtttc tagaagtatt tttcaatcaa tggagatgaa
    42601 atataatttt aaagtcaatg cttatgagct ttcgctgctc tatgaattat taggaccgta
    42661 tattcaaaaa taaagtgtct aaaaaagtgt ctaatctgtt ttttttaggt tagacacttt
    42721 ttttaatgtg aattaacgtg attaaaccaa tgtttttaaa agttggcaca ggaattgctt
    42781 atataaaagt gaacagaaag aaaggaatta accatatgaa gaaaattcta atagcgactc
    42841 acggtcactt agcttcaggc tttcttagca gtattcaatt actgacgggg aaggttcaag
    42901 agattacggt gattaatgct tatgtggatg agtgtgattt tgaacaggag ttgaatgtat
    42961 ttgtaaacca ggtgaacgag caggatcaga tttttgtttt taccgattta tttggcggaa
    43021 gtgtcaatca gaagattacc aaaacctttt tggagaaaga gattgctgta actattttgt
    43081 ctggctttaa tctgccaatt ctgctcgaaa tagtattggc aaccaaggaa ttaacaaagg
    43141 aggatattca ggaactggta aataaatgcc aacaggaatt aaagatgacc attcttgatg
    43201 aggtacatgt agtaaatgaa gaggaagatt ttttttagga ggaagaaaaa tgattaaagc
    43261 agtgcgaatt gatgaacgat taattcatgg acaagtagcg atgacttgga caaagagtct
    43321 aaatttaacc gggttagtag tagcaagcga tgaggcggct accaacgata ttcaaaagat
    43381 gacattgaag atggctgcac catcgaatgt caaagtcatt attaaaacag ttgatgacgc
    43441 gattcatttg ttaaaagatc cgcgggcgaa ggacatgcgc ttgatggttt tagtccctaa
    43501 tgtcaaagat gcggtaaaag ttgcgaaagc tttcccaacg gaaatcgaat taatgaacgt
    43561 ggggaatgct gggaaaatga caccgccatc ggaagataag aaaaccttat caaaggaagt
    43621 gatgttaacg acgtcagaaa ttcaagactt aaaagaatta gttgaacttt atccagatac
    43681 cgtttttcag cctacaccgg cgatggaaaa acgttcagct atctctattt tgagtgattt
    43741 ttagaaaaag gaaggagaaa aattatgtat gatgcatttg tagtcgcttt ggccgttttt
    43801 attggaattg ctggacatga aatgttcggg atgtccatgc tgagtcgtcc gattgttgtt
    43861 gctccgttag caggagcgtt gatgggggat ttgcagacgg ggttgattgt cggggcgtca
    43921 ctagaagcca tctttatggg tgtcgtcaat atcggaatct ctagtacggc ggagccttct
    43981 ttggctgctg ggctggctac agcgttcgca atcaaaatgg gaggaaacgt ggatgcgatt
    44041 attcccattg tctttccact agcagtatta ggattacagt taatgaatat gattttttct
    44101 tttgtatgtg gaccgatggc tgctaaattt ttcaaatttg caaaatctgg tgatcaaaaa
    44161 ggaatcatca atctgcactt ctttatgtgg tttgtccact atgcgcttta tgctcttatt
    44221 ccattctttg ccgttttatt tggagcagat gttgtcaaaa gcgtcttgga taatattcca
    44281 gaggtcatca tgaacggatt aacagtcgct ggaaatctgc tgccggcagt tgggatggcg
    44341 atgctgttac ggatgctttg gagtaaaaat attgctgtgt ggtttttctt tggggccgtt
    44401 gttatggcgt atctcgagtt accattgatt gcaattgccg cagttggaac gattattgca
    44461 attgttatag cccaaagaga tctacaacta aaaaatatat ctgttcatgc agttcaaagc
    44521 gatgctgctg taaatgcaac gcttgaccaa gaagaggagg atttctttca atgaaattag
    44581 ctgatggaat ttctaaagat gagaaaaaat tggttcggaa gatgttttgg cgctcgatga
    44641 ctatgtatgc ttcggtgaat ccggtgacga tgggcggcgg tggtttcgcc tattctatga
    44701 tgcccttcat taatcatttc tacaaaagtg aagaagatcg agctgcggcg ctggaacgtc
    44761 atacacagta tttttcgacg accattcctt gcgcgtcctt tgtcatgggg attgcggcat
    44821 cgatggagaa ggaaaattct gaaaaagctg gttttgacgt ggaaagtatc aacgcgatca
    44881 aaacgagttt gatgggacca ttggctggaa ttggcgattc attgttctgg ggagtttggc
    44941 gtgtcgttac cgcgggacta gcgatcaact tagccaatgc cggaaatatt ttggcaccaa
    45001 ttatcttttt agttatgttc aatataccta attacttgat gcgatactat ggcggcgttt
    45061 taggctattc attaggctct aaatatattg aaaagctgta tgcgaacggg ttaattgaaa
    45121 ttctgacaaa agctgctagt atcttgggtc tgatcatggt aggtgcaatg acgatcagta
    45181 tggtcaaatt caataccgtt ttgagctttt caatgaaggg cgaagaacta atgaatgtcc
    45241 aagaaatcct cgatagtatt tttaaaggaa ttgtaccgtt attggtaaca ttgagctgtt
    45301 tcaaattact agataaaaaa gtcggcgtca tcaaaattat ttttggaatg gtcgtcttgg
    45361 gaattttatt gtcattactt ggaatcgcat agtaggaagc tgcagctact aggctgcagt
    45421 ttttttagca aggagggaaa agatgactat taatgaagta atttattcac ccaaacaccc
    45481 attttttaat gctgagttaa acgacggaaa tgtgactact tcaaacgaat acagctacta
    45541 tcggaatatt cctgatgtaa agggattgac cttcgatgta agtaatcaaa agacattagc
    45601 aaaccttgat gtttgcggga cgattcaaac gttgaccttt tatcgagaaa atttactaac
    45661 tgaggaaaaa ccaggtgttt gggtcaataa acagctctct caaaccaatt cgttatcctt
    45721 aaaggttgaa gtaaacggga ttgtctacca tttgtcagaa tcggatcatc gtattgaagt
    45781 cgatgtgatt caagactgtt tgcctagata tattcatcat tattcgacgt ttaaggtcat
    45841 tgtagtatcc tttgctccca tactggctaa taagcgttta agtatgcttg ttcaacaggt
    45901 ttacattgtg aatgaaacac acgaaagcct tcaagtttca gtgaaagatg tgccttcgta
    45961 tcaagagaag tattcggatc agcaaaatgt gttgatttct caaaaaggaa ataaggacac
    46021 gatcgcacaa caggaagtgg caagcttcgc cattgctttt gtggacccaa atgcttttga
    46081 agaagtaatg acttttcagg aatctgatat cgaaaaatgg ctgaaggaaa cattgaacta
    46141 ttttgctcag ctatttgggc agctgtcaat gccaaacgat agagttgtcc atctctatcg
    46201 tcgtgcactt tatcaaagct tttctagttt cggtatgaat cgacagggac aggtcgtcgg
    46261 cagcaattgg ggcagctatc ctgctactaa tcgaatttgg aataaggata tgtattacgc
    46321 atcattgcca ttcaccatgt ttgaaccgga gttatgtcaa aaaacggttc tatggtttga
    46381 tcaatatggc gttaagtttc ctggttcaaa atttacagga ggtatcaatc attctctgag
    46441 taattcgtta tccagtgcgt tactggctag tctttatttt gaacatactg gggatacaaa
    46501 attctttgag cggtgtccgc aggttctact gaatgcttca agaattattg aggacatatt
    46561 agctcaacgt cggtcagggg aaccaatgct ttttccatct gtttggttat cagacgcctt
    46621 tgcacttgga aagtatcata ccggcagcaa tctttgtctt tggaaggcct gtattggact
    46681 tgcagaaatt tttgaagccc tagaacaact gagtcttagc aagcgataca aaaacattgc
    46741 ctgtaaacta aaggaatcaa tttgtaaaca aatgactatc gatggtcctt ttggtgagca
    46801 atttttggag ggaatcggtg atgaggagaa aacaacctat tcagtgaagc actatcaaaa
    46861 accgattctt gagcaagggt taatcttttt aagtgatgtg attgtggatg gaaaaattaa
    46921 tttattgatg catgatggag aagaatcaga cactacgttg atgccttttt atcaattttt
    46981 agataataaa gatcatttat atcagaacac catggccttt tctgctagtt ctttcaatcc
    47041 aacctatagc gaggaaatca aaggcatcac ttggggatta gaatccggcg ccacttttcc
    47101 tggctttatc actgttttaa tgagcgagct gcacaaccct caagctttcg taacccgatt
    47161 agaagaacta atttgtttgg cagatttaga cggttcgtgg tggtggtggc cgtatcgctt
    47221 agaggctcaa caaggagaag tcgtgcgaga ctttggctgc ggcaaatgcg gctgggcatc
    47281 gggtcttttt gtcagcctaa tgatcagtca atatttcgga ttgaaattca aaaaaggagt
    47341 attgcaaatt gatcctgttg ataatttaac gttttcttgg aagaatttga agttcggtca
    47401 agccttcatt tcgattgaat gtacgcaatc cgagttgagt atttcaaatt tgtcagaaaa
    47461 gccattgaaa ataagtgata cgaaaaaaat tcttcacgta gaaaagggaa aaacgattca
    47521 cattcaaagg agaaaaagat gagtatacta aaaaatatta cccaatttaa aaaaatccag
    47581 agtcgagcaa tcactcccga aaatccaaca ggcgagaaag gtaaagctgc gatgttgcca
    47641 agcgagctag gaccaagccg caaaggtcgt ggcgcaattc cgctgacttc tggtgaagaa
    47701 actgtgattg cggatattac tggaacggga ataattcgtc atatgtggat gacgattcgt
    47761 gatcgaacag aatttggcag ttttgtttta cgtgatttaa tcttaagaat ttattgggat
    47821 gggtcggata gccctgctgt tgaaagtcct ttaggggatt tcttttgtaa tgggtttgga
    47881 gagcgttgcg aggtgaactc gttgccgata gtggttaatc ctacaggggg ctttaattcc
    47941 tatttcgaaa tgcctttcca aacatccgct aaaattacga tcgagaattt acatccgaag
    48001 gatatgtcct cattttttta tacgataaat tacgctttgg tggacgaatt ggaagaaaat
    48061 agcctatatt ttcacgcaaa ttggcatcgg gaacgcatta ccaaattaca ggaggattat
    48121 aagttaattg atcgattaaa gggtcaaggc tattacgtgg gtacttacct tgcgcttacc
    48181 tgcctagaac gttattggtg gggcgaaggc gagtttaaat tctacattga tggggatagt
    48241 gagtatccga ccgtaacctc aacaggttca gaagattatt ttggcggggc gtgggccttt
    48301 catgagtacg atgagttcgg acgtccacat gcgaaaaact attcaacagc ctttttaggg
    48361 tatccttacc aatcaaatag agatgccaca agagatcgtt ttgaaacggg aaaattgaat
    48421 gccgttcatg cctttggtga cgacgcaatg ccacgacatg gtctttatcg ttggcatctt
    48481 caagacccca tctgttttac cgaagattta tcggtcactt tacaacaaat cggcaacgat
    48541 gatgttcgct tatttgagcg gcaagacgat gttgctagtg tcgcttactg gtattcaaca
    48601 gaagcttcag gaaatactaa acgtcatttc actgatcgag aacgcttgcc gcgttaatga
    48661 agtgtttttc acatattcga agggaggaac ctatgatgat atatggggca attgaagctg
    48721 gagggactaa atttgtctgt gcagtcggta ctgaggaatt aacgattttg gaaagagtca
    48781 gttttcccac aacaactcca gttgaaacaa tgaagcaggt cattgatttt tttaaacctt
    48841 ttaaggaaca attggcaggc atcggggtcg gctgctttgg tccaattgat attcaatgca
    48901 aatcaacgag tttcggtcat atcacatcaa caccaaaact tgcttggcaa aatttcaatt
    48961 ttgtaggaac gctaaagcaa tatttcaata ttccgattga atggacaaca gatgtcaatg
    49021 cggcttgcta tggtgaatat gttgctggtt ctggcaaagg gttagcaggt gttgcatact
    49081 tcactgtggg aacaggtatc ggaggtggtg ccttgttcaa tgggactttc gtggaagggt
    49141 tcagtcatcc ggaaatgggg catatgttga tcaagcgaca tccaaaggac aatttttcag
    49201 gaaactgtcc cttccaccaa gattgtttag aggggatggc agcaggacca gcgattgaaa
    49261 aacggttggg tgtaaaaggc cagaatttat tggctgacga ttcgttttgg cagattgaag
    49321 ccttttacct tgcccaatgt gcatacaata caacactgat gttttcgcca gatagaatta
    49381 tttttggcgg tggtgtaatg aaacaagaac acatgaagaa aaaagttcag gataaatttg
    49441 ttgagcttat aaatggatat gttgaaatac caccaattga tagctacatt attacgcccg
    49501 aattaggtga taatgccgga ataatcggcg gattagcatt ggccagaaaa gcggtaagaa
    49561 ataagcaacc ataattaatg aatgtttcat tgatactcat gtcaaaagta tttaaccgag
    49621 taatcaaatg atcaaatgaa gttggataaa gcgaaaatga attcaaataa aaaggctcta
    49681 agtcaaatcg tgttgttttc attaaatcac tgaaaggaaa tgcttctgcg tttccttttt
    49741 tctttttgtt gtgcgctgta taccttgagg atattcactg gtggcgtaca cttaaatagt
    49801 aatatattgt gttactttat gtctgtggta atagttatag ttgcttacca atgtatgttc
    49861 atttttcgaa atgttgactt cataatcctt ggcttaatgt ttgaattttt ttggtgatag
    49921 attttttcag ccgtataaaa taaatcgtaa ttctattata gagagttgtg attataaaaa
    49981 gatagcaaga aaaaaaaaga aagtttctat acattttaaa tattttattc ctatttctat
    50041 tgctaaaaaa gtagaaattc tatatttacc tttaactctc gcttctcttg caaatgtagt
    50101 tagcatttta atc
//
LOCUS       DAZULO010000010        49558 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000010 DAZULO010000000
VERSION     DAZULO010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.010"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..589)
                     /locus_tag="ACG8OA_000611"
     CDS             complement(<1..589)
                     /locus_tag="ACG8OA_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HFS4975454.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVI"
     gene            complement(603..1499)
                     /gene="murQ"
                     /locus_tag="ACG8OA_000612"
     CDS             complement(603..1499)
                     /gene="murQ"
                     /locus_tag="ACG8OA_000612"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS4975455.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(1502..2563)
                     /locus_tag="ACG8OA_000613"
     CDS             complement(1502..2563)
                     /locus_tag="ACG8OA_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFS4975456.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(2774..3301)
                     /locus_tag="ACG8OA_000614"
     CDS             complement(2774..3301)
                     /locus_tag="ACG8OA_000614"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4975457.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(3368..4870)
                     /gene="eat(A)"
                     /locus_tag="ACG8OA_000615"
     CDS             complement(3368..4870)
                     /gene="eat(A)"
                     /locus_tag="ACG8OA_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS4975458.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(5128..6249)
                     /locus_tag="ACG8OA_000616"
     CDS             complement(5128..6249)
                     /locus_tag="ACG8OA_000616"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS4975459.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            6375..7316
                     /locus_tag="ACG8OA_000617"
     CDS             6375..7316
                     /locus_tag="ACG8OA_000617"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS4975460.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(7466..8362)
                     /locus_tag="ACG8OA_000618"
     CDS             complement(7466..8362)
                     /locus_tag="ACG8OA_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS4975461.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            8631..9971
                     /locus_tag="ACG8OA_000619"
     CDS             8631..9971
                     /locus_tag="ACG8OA_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4975462.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            10189..10632
                     /locus_tag="ACG8OA_000620"
     CDS             10189..10632
                     /locus_tag="ACG8OA_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFS4975463.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(11136..12554)
                     /gene="nhaC"
                     /locus_tag="ACG8OA_000621"
     CDS             complement(11136..12554)
                     /gene="nhaC"
                     /locus_tag="ACG8OA_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS4975464.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(12957..14180)
                     /locus_tag="ACG8OA_000622"
     CDS             complement(12957..14180)
                     /locus_tag="ACG8OA_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS4975465.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(14211..15050)
                     /locus_tag="ACG8OA_000623"
     CDS             complement(14211..15050)
                     /locus_tag="ACG8OA_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4975466.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(15067..15747)
                     /locus_tag="ACG8OA_000624"
     CDS             complement(15067..15747)
                     /locus_tag="ACG8OA_000624"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS4975467.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(15749..16822)
                     /locus_tag="ACG8OA_000625"
     CDS             complement(15749..16822)
                     /locus_tag="ACG8OA_000625"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS4975468.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(16878..17116)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(17159..17392)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(17469..18140)
                     /locus_tag="ACG8OA_000626"
     CDS             complement(17469..18140)
                     /locus_tag="ACG8OA_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4975469.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(18227..20875)
                     /gene="mgtA"
                     /locus_tag="ACG8OA_000627"
     CDS             complement(18227..20875)
                     /gene="mgtA"
                     /locus_tag="ACG8OA_000627"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFS4975470.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(21217..21391)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            21564..22001
                     /gene="ndk"
                     /locus_tag="ACG8OA_000628"
     CDS             21564..22001
                     /gene="ndk"
                     /locus_tag="ACG8OA_000628"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFS4975471.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(22023..22889)
                     /locus_tag="ACG8OA_000629"
     CDS             complement(22023..22889)
                     /locus_tag="ACG8OA_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4975472.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(23049..25142)
                     /locus_tag="ACG8OA_000630"
     CDS             complement(23049..25142)
                     /locus_tag="ACG8OA_000630"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4975473.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(25891..26514)
                     /locus_tag="ACG8OA_000631"
     CDS             complement(25891..26514)
                     /locus_tag="ACG8OA_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975474.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(26673..28070)
                     /locus_tag="ACG8OA_000632"
     CDS             complement(26673..28070)
                     /locus_tag="ACG8OA_000632"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4975475.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(28102..29073)
                     /locus_tag="ACG8OA_000633"
     CDS             complement(28102..29073)
                     /locus_tag="ACG8OA_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4975476.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            29447..29872
                     /locus_tag="ACG8OA_000634"
     CDS             29447..29872
                     /locus_tag="ACG8OA_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975477.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(30360..31061)
                     /locus_tag="ACG8OA_000635"
     CDS             complement(30360..31061)
                     /locus_tag="ACG8OA_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS4975478.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(31077..31517)
                     /locus_tag="ACG8OA_000636"
     CDS             complement(31077..31517)
                     /locus_tag="ACG8OA_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS4975479.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(31517..32092)
                     /locus_tag="ACG8OA_000637"
     CDS             complement(31517..32092)
                     /locus_tag="ACG8OA_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFS4975480.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            32333..32542
                     /locus_tag="ACG8OA_000638"
     CDS             32333..32542
                     /locus_tag="ACG8OA_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFS4975481.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            complement(32940..33452)
                     /locus_tag="ACG8OA_000639"
                     /pseudo
     CDS             complement(32940..33452)
                     /locus_tag="ACG8OA_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(33578..34279)
                     /locus_tag="ACG8OA_000640"
     CDS             complement(33578..34279)
                     /locus_tag="ACG8OA_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4975482.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(34387..35830)
                     /locus_tag="ACG8OA_000641"
                     /pseudo
     CDS             complement(34387..35830)
                     /locus_tag="ACG8OA_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            complement(35844..37709)
                     /locus_tag="ACG8OA_000642"
     CDS             complement(35844..37709)
                     /locus_tag="ACG8OA_000642"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4975483.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(37830..38681)
                     /gene="licT"
                     /locus_tag="ACG8OA_000643"
     CDS             complement(37830..38681)
                     /gene="licT"
                     /locus_tag="ACG8OA_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFS4975484.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(38991..39704)
                     /locus_tag="ACG8OA_000644"
     CDS             complement(38991..39704)
                     /locus_tag="ACG8OA_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFS4975485.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(39772..40098)
                     /locus_tag="ACG8OA_000645"
     CDS             complement(39772..40098)
                     /locus_tag="ACG8OA_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS4975486.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(40221..40448)
                     /locus_tag="ACG8OA_000646"
     CDS             complement(40221..40448)
                     /locus_tag="ACG8OA_000646"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS4975487.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(40770..42023)
                     /locus_tag="ACG8OA_000647"
     CDS             complement(40770..42023)
                     /locus_tag="ACG8OA_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS4975488.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            42084..42824
                     /locus_tag="ACG8OA_000648"
     CDS             42084..42824
                     /locus_tag="ACG8OA_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS4975489.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(42910..43668)
                     /locus_tag="ACG8OA_000649"
     CDS             complement(42910..43668)
                     /locus_tag="ACG8OA_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS4975490.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(43665..44129)
                     /locus_tag="ACG8OA_000650"
                     /pseudo
     CDS             complement(43665..44129)
                     /locus_tag="ACG8OA_000650"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            complement(44148..44645)
                     /locus_tag="ACG8OA_000651"
     CDS             complement(44148..44645)
                     /locus_tag="ACG8OA_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFS4975491.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            complement(44713..47880)
                     /locus_tag="ACG8OA_000652"
     CDS             complement(44713..47880)
                     /locus_tag="ACG8OA_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFS4975492.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(48104..48988)
                     /locus_tag="ACG8OA_000653"
     CDS             complement(48104..48988)
                     /locus_tag="ACG8OA_000653"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4975493.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(49083..49520)
                     /locus_tag="ACG8OA_000654"
     CDS             complement(49083..49520)
                     /locus_tag="ACG8OA_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975494.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT        14078 a        10460 c         8466 g        16554 t
ORIGIN      
        1 agataactag gtagctaaac atcccgtttt tcaagatgtt catcacatcg atatattgtt
       61 gccatgttgc tgcatcaagt gttccggctg tgactaagtt tgctaaaact gcggcaatcc
      121 ctgccacgat cccagtaccg acaaatgcag gaatcattgg cacaaagata ttagaaatgt
      181 cttttaatac cgatttgatt ttagatggtt tttgtttcga tttttgtgcc gctttcattt
      241 cttgtgcccg ttcgtttaca cggtcttttc cagatgctga tgaatttgct gtagctgtac
      301 ttacagaaga aagtttttct cctaatttca cgcctgccaa atcaaccatt tgctgtgcta
      361 ctttgttcac tttcccaggt ccgatgatga cttgaagctg ttcatcttct actacaccaa
      421 gcactcctgg aatcgctttt aattcagaga catttacaag atcatcattg atgatcttca
      481 tacgcacacg agtcatgcag tggacaatgc tttctacatt gcccatgccg cctgcccctt
      541 tataaatttg ttttgccagt tcttccaaag tcagttcgtt ttttgccatg gtcttttccc
      601 cctcataagg tcccttttac aaatccgttt gcttcagcta attttgcttg agcttcctct
      661 ttatcactat ttgtcaaaat catgacgata gccagcttaa cattttgatc tgcttcttca
      721 aagtactttt cagctgtttc gtaatcagct tctgtcgctt gcatgatgat tcgttttgag
      781 cgttcgatta gtttttcatt tgttggtttg acatcgacca tcaagttatt gaatacttta
      841 cctataccaa tcatactgat agtagaaatc atattcaaga taagtttttg tgctgttcct
      901 gacttcaatc gagtagagcc tgtcaaaaat tctggacctg catcgacttc gattgggaaa
      961 tccgcgtgtt tactgatttc ggcttgttta ttgcaagaaa tcgttcctgt gacagcaccg
     1021 atcgattttg cgtaatctaa tccaccgatc acatatggtg tacgaccgct agctgcgata
     1081 ccaataacta tatcattttt tgtcaaattc agcttgatta gatcttcttg ccctagctca
     1141 taagaatctt cggccccttc gacagctaca gtcattgctt cttctccacc agcaattaga
     1201 cctatgacca tttccggttc tacaccgaat gtcggtacgc attcagcagc atctaacaca
     1261 cctagtcttc cacttgttcc ggctcccata taaatcagac gtccaccttt tttaaaggac
     1321 tcaatcgtgt tagcaatcac tggttcgata cttgtcagtt ctttttcaac cgcagcggcc
     1381 actttctgat cttctttgtt cattagttga acggcttgag aaatactcat ctcatctaat
     1441 ccaaaagttt cttggtttct gcgttccgtt gttaggttct ctaaattgat tttgctcatt
     1501 tttatcttcc ttcttttctt ataaattgat agttcatacc agctttgatt tggttgatca
     1561 atggtagctc ttctttgata actttagcta ctacattcac tttttcatcc gctggcaaat
     1621 catatttggt taattggatc tcacccatat agcgcaaata cttttcgtta tctaaagtaa
     1681 tagacccttt catacgcgta gctgtatttc ttgctgggat aggcggaatc tctcgaaaac
     1741 gtgcatctac gcttcgtatg acatctcttg catcgtcttg ccgattcgta tgcttcccta
     1801 atatatattc gtaaaattct tcatctacag gttccgtatg gagcaagact gtctgttctt
     1861 tttggaaaga agcaaattgc tcctgaacgt tttctgacag acctgcatct ccgatgtaaa
     1921 caatatccgt gttacttgct aatagatcta gagcgcctgc aagaggatgg atccctcgat
     1981 gttcttccaa agttggcaat ccttgatata gtgggcctct caactctgta tctcctggta
     2041 caaaaccttg gacaaggaga ccttgaattt tgagccattg attttttttc tgataccatt
     2101 ctttgtccag ccctgtttcg ggacgtggat aataattatg ccaagcttca atctgtgaga
     2161 aatcagcttc tgcttctttc aattcatcga tatctttagg cgtgatcgtg ctagcattca
     2221 aactgatttt caattgatgt gaccagtcgg caatctgctg attggaaata tgataatcca
     2281 ttcttaaacc tgttacacca attgctttca aaggcgttag atcttctaat gaaaaacccg
     2341 ctcgttccaa tgcctctcct gaaatatcga ccatcaattc tagtttattc tttttcgctt
     2401 cgcttcctag ttcgatcaat cgcttttgat atgccgaaac atcatcttct ggtatatgca
     2461 tggaagtgaa aataccgaca aagccattgg atgccatttg gcggatatac tgtttcttct
     2521 cttctgacaa agaatcgttc ataaatattg aaaagccaaa catattatcc ctctttttct
     2581 gtatccgtct tcatttctat gactagagta tatatctaaa gaaacaaaat ttcaatactt
     2641 ttattaaaat aattaaattt catttctaaa ggttgagttt atattatcaa aagaattgtc
     2701 ataacctaaa aaaggcactg ctaagcaaaa gcagtgcctt gaaatatttt atttaaataa
     2761 cacaaaaaca taattactca taagaaacca attctgtatt atctaccatg aatgtaaaga
     2821 acccctcatt aggcatcgga tgaaaataag cattgatcac tcttatcaag gcattatggc
     2881 aaactaacag atagacattt tcttcgtctt cgatgcattc tttcaataaa ggaacgatcc
     2941 gtgcatacac atctaagact gactccccat ttgggaatcg tacagcgaac gctaaacgtg
     3001 ctttttgaaa gttctcatct ttgcttggca gtccatcata atccccaaaa tccatttcta
     3061 ccaatcgttc atcaacggtc agcggcaagc tgagacgatc agcaactgct tgtgctgtat
     3121 ctcttgctcg ttgaagaggg gaatggatga ttttcgtgat tggaacaggc aaatctacta
     3181 ccttttctgc tagtttctgt gcctgcatct gtcctttttc agtcagcggc acatctgccc
     3241 gtccgcaaac taatccttgt gcgttccaag ttgtttctcc gtgtctcgtt acataaagca
     3301 tgtgattctc tccttcgttg gcaaagttcc ccttaaccat accttgttgt tcttgagatt
     3361 acaagggtta gattctctca agagaaataa tctcagtaga tactttatcc aggaaggttt
     3421 gatcatgttc cactagtaac atggcaggtt tcacgttcaa gatcagttgt tctaattgtt
     3481 cttgattgaa gacatccaaa taattcaatg gttcatccca tgtatatagt tcagctggct
     3541 gtgataaaga tttagccaat tccacttttt tccgttggcc catactcatc tgctcgatct
     3601 tgttatgaaa aacatctctt tccatcccaa gctttcggag gttgttcaaa aatgcttggt
     3661 agtctacttg gtttttctct gcaaattccg ccaacgttcc tcgattgtct tcataatttt
     3721 ggcttgcata actaatgctc agatgttttg gctggtattt ttctcctata accttgccgt
     3781 tgaatgcccc cagaagataa tggataatgg atgactttcc tgcaccgtta ggacctgaaa
     3841 tcgccacccg ctgatgaggc tcgattgtaa aatgaattgg ctcgaataac agattttcat
     3901 accctaattg aagatcttct acgcttaaaa gtcgcttatg gtgagacgct tggctattca
     3961 tcgtcaaaga atcgatatat tcgatatctt ttaatagttt ttctttctcg ctgatctggg
     4021 tctccatccg attcacgatt gctttggatc gtttcatcgt ccgtgcagca tcagcaccca
     4081 ctgctccttt attcactcgt ctagattcag tatcgatgaa tccgacttgt ttctttgttt
     4141 tatctccttc tcgggaacga gaccattcgg ctttttcagc tgctgttttc tttagcctac
     4201 tgacttcttt tttcaatttt tcattttgag ccatttcaaa ttcatcacga agttttttct
     4261 gttcttcata gatagagaaa ttcccttgat aaagttccag ttgacttttt tcgattgcta
     4321 aaacatggtc cactacttca tcgataaatc cccggtcatg gctgaccacg atgaagcctt
     4381 gttttttctt tttcaaataa gccgctactt gttttctacc agagatatcc aaatgattcg
     4441 ttggttcatc gattaacggg aaatgagtgt catccacaaa taaaagtgcc agtaggactt
     4501 ttgtcttctc tccccccgat agtgtgctga attctctcca taagatttca ggatctgttt
     4561 gcatcaattg gagctctctt tcgatttccc atatctcaaa atccgtaata tcatttaaca
     4621 cgtaataggt taaacgttct ttatcttttg tctgttgcgg gaaataggca aattcttgct
     4681 gatggattac ttgcccttga taaggtagtt tgttttgtag aatattcagt aaagttgtct
     4741 ttcctcgacc gtttcgtccg ataagtccta gtttccattg tgtgtcaaaa tttagatttg
     4801 cttgttcaaa taataatgtg ccttggctgt cgtagccgaa tgtcagattt tttatttcga
     4861 ttttagacat tttccatcac ctcgtaaaaa aagaccagtc ctattttcag aactggctaa
     4921 agaggatgca agggttggat acacacaaaa agacccatac atcgaaaaaa ccaagccaat
     4981 cccgaaaatc tgcacactgc ttccttgtct ccacaaggta cagtctgaat gcagctttca
     5041 tcaatcgact taatttttca atgcacggat ccctcacttt gtcctatatt tgaaattacg
     5101 atagcataaa gaaaatgatt ttgtcaacta cttcgggagg atggtgaaaa atctgctaag
     5161 tgtctgtcct aagatttttg ttttttctaa tgtttgcctg tgttccattg cttcttctaa
     5221 acctattgga cccaaatgaa tacttaagac aattgggaga aaatcagaaa gtccttctag
     5281 ctcttggtct ctacttccgc aaatagcgat tattggtttt cgatattttt ttgcaagcac
     5341 tgctacaccg tatggaactt ttccgtggat cgtttgctga tcgatccgcc cttctcctgt
     5401 aatgatgaca tctgcttgtt cgatcttttt ttccaatcca gtgatttggc tgactaaatc
     5461 gaaaccagaa accatcttcg ctcccaacaa aaaaagggct ccaccaatac cgcctgctgc
     5521 tccacttcct ggaatatcat ctaaatccac ctcccagaaa tcttttattt gctgagcaat
     5581 ctttcctgct tgtttatcta agaaagctag cgtcgtatct attcctcctt tttgtcttcc
     5641 aaattgccaa gcagctcctt gttgacctgt atatggattg gtaacatctg cagcgataat
     5701 caattttact ttagaaacaa gaacgtttct tcgatcaaaa tgaagttttt tagtagataa
     5761 taaaggattt ccttctgtgt acccttctaa agaagcaccc aaggattgga gtaatcctag
     5821 accaccatcg ctacacccgc ttcctcctaa agtgacaata atttgttcta cttcttgaag
     5881 cagtgcatct ttaataagtt ctcctaaggc ataactggaa gcaagtcgga tagtttgatc
     5941 cgttggttta tcaagtagat ctaaccccaa tgcttgtgca gattcgatga ctgcggtgtt
     6001 ttttccttgc caagttgtca ataaatagct gatttttcgc tgacggaata agagatccat
     6061 cccatcaatg gtacgccatg ttcccctgag cgcgtaatag attgcttctg cagtattttc
     6121 tccgccatct gcaatcggca cattgatttt tcttccttta cgatcttccc agccatttag
     6181 aactgcctga tttagttcaa atgaagaagc acttccttta aatgaatcaa ttgccgccaa
     6241 aatatccatc tgtatccctc actttcttct taagtaagac actttttttc atttttttca
     6301 aacaaaacaa tgctattttc ttccattttt ctatatacta aaagggaact cattgaataa
     6361 aggagagaaa aaacatgaaa atcgcaatag ctggggccgg agcaatgggc agccgaatcg
     6421 gattgatgct acatcaaagc ggaaatgagg tcctcttgat cgatcgttgg cctgctcaca
     6481 ttgaagccat tcgtacaaat gggttgattg ctgactttaa cggtaaagaa gtggtcgcaa
     6541 aactgccaat ctattcacca gaagaaatca tcgaaagcaa cgaacacgtg gacttgatcg
     6601 tagcattaac aaaagccaat caattagatg atatgttttg ttcgatccaa tccattatta
     6661 ctgacaatac ttatgttttg tgtttactaa atggattagg tcatgaagat gtactggaaa
     6721 aatatgtgcc aaagaaaaat atcttatttg ggatcacgat gtggacagct ggtctagctg
     6781 gtcccggaaa agtcacattg ttaggtgatg gagaaatcga actagagaac ttggaaccag
     6841 aaggcgaagc atttacaaaa aaagttgtag aagtctttca agaagcaaat ttaaatccga
     6901 tctatagcca taacgttcgt tattccattt ggcgaaaagc ttgtgtaaat ggaacattga
     6961 atggtttatg cacgattttg gattgtaata ttgctgaact aggcgcgcaa aaagcggcag
     7021 agagcatggt tcgaacaatc gtatctgaat ttgcttctat cgcagcaaaa gaaggaatca
     7081 tactagatca agaagaagtg taccaacata ttgcttctac ttatgatcct gacaacatcg
     7141 ggttgcatta cccatctatg taccaagacc tgatcaaaaa ccatcgtttg actgaaatcg
     7201 actacatcaa tggtgccatt tggcgaaaag gacaaaaata tgatattgct acaccatatt
     7261 gtgccttttt gacccaatta gtccatgcaa aagaaggaat ccttggcgca gaataaaagg
     7321 ctctctgatg agaatcagct gaatcagcag ctggttctct ttttttaagc ccaataaaag
     7381 atcaaaaaga aaacacaact ctcatgatgc cataagaatt gtgcttgttt gtttcagtac
     7441 ataatctctt caaaatgtag gttttttatt tgttttcaac agaggagagg tcatattcgt
     7501 attgctcata aatagtggaa tcgttagtga taaatcgttt tgcccaattt tctgcatcat
     7561 aaaattccaa tagctctgat aggattggct taaatggtgt atgcagaaat tccggtaatt
     7621 catctaaatc acgttctcga agcaaaaaaa tattttcagg acgataaaaa tgataatgag
     7681 caatcgcttg attatccgtc actagtttct tttgaaaata catggattca aatgtccgca
     7741 aagtcactcc ttgctgaccc tcccgcaaca gttccagaat cccgcgcgat tcccctgttt
     7801 ttttcaggta ctcttcataa gtaagatatc caggttgttc attccctcga tgtggaagaa
     7861 tgaaatagtt ggtcgtaaca tctcttttta taaattcctg cttatagctt tctgctcgtt
     7921 cttttctccc attattcgtt gcaccaaaaa atagatccgt ctttggttcg ctagaagaaa
     7981 aattcatatg tttcgaataa aatgaactat tatgtttcag cccaagttct tttgcttcca
     8041 aaaaatcaaa atgataaatc tcatcgattg ccggttcatc ttgtgcctgc ttcagcatcg
     8101 cctcgtgttc tgctttgaag tgattccaaa aaaataacac aatttttgca ttggtaaatg
     8161 ggcgcacgcg tcgagccatc tccatatcaa atccataatc taaaaaaacg atcagctgat
     8221 aatttgccaa ctgttgtcta gctgatttaa tgaattccga ctgtagtaaa tcttttccgg
     8281 accaaacatc taaagagcgg atggaaggca aaaaataaag tgatttattt tttgcaacta
     8341 gcagtacgtc atttttgttc atcttttccg cctcactttc tctgtcactt tttcctatga
     8401 tagcctgttt aaatagtaaa aagccagtaa aacgtttcct atatttgtat gaaaatttaa
     8461 catttgtatg aaaatatgat gaaataaatc attcttctat taaaattcgc ctaaaagttt
     8521 tctgaatagt tgtaattgtc tgaaaatttg ttatcattag tcattgttaa atattgctat
     8581 ttattcttaa tataaatttt gattgtgaat ttaaaagaaa gaggtcattt atggaagata
     8641 aaaaattgaa aaaacaactt agctccagac atatcaccat gttagcttta ggtggagcaa
     8701 tcggtgcagg tctatttaaa ggaagtggcg aagcgattgg gatcgccggc ccttctgtat
     8761 tgattgcttt tttgattggt gggctgattt tatatatcgt gatgaaaggt ctgggaaaaa
     8821 tcgttttaag cggtggcgac acgcatcacg gattatctgg tttgatacgc ccttatctag
     8881 gtgctcattc tgctgatttt actgattggg tctactggtc tatgtggatt atcaatatca
     8941 tcgctgaagc tgttgcagct gcttcctttt tgcagttgtg gttcccgcac attccagcct
     9001 gggtatttgt ttttatgttg gctgttttaa ctacgatcat caatctttat tcggtacggt
     9061 tattcgcaga aacagaatat tggctggctt ttgctaaaat cagtgtcatt atcttactga
     9121 tcatttttgc tacttatctt gtcggtcagc aaatgttggg cacaggcgtt ttccctaccc
     9181 ttcagcaatt gactgaccat ggtgggttta ctcctcatgg tatgaaggga atcatcagtt
     9241 ctttgcttgt agttatctat tcttacggcg gttctgaact gattgctatc acggtaagcg
     9301 aagcagatga tcctaaacgt gcgattccta aagcaatcaa aggtgtcatg ggaagaatca
     9361 tttcctttta catcattcct ttgtttttgc ttttgattct ttttccttgg aatactttag
     9421 caggcacgaa tgtcagtcct tttgtgatgg tatttgaaaa aatgaatatc ccatttgcag
     9481 cggatatcgt caattttgtg attgtccttg ctttgttctc atctatcaat tcgggtgtgt
     9541 atgcatcttc aagaattttg ttcttccgtt tgaaggatcg aaaaggttcc tctagaaaac
     9601 tagctgtttt aaacaagcat caagtaccgc aacgtgctgt tttattttgt accagtgttc
     9661 tttatcttgg tgtagctctt tcttattttg tcggtgacaa attatttggc tatctggcag
     9721 gttccctttc gtatacagta ttattgatct ggatcattat cagtgcggca tcctttgtac
     9781 tggcactaag aaaaggaaca ttatgggata aaggaatcag cttttttgca ttagctgtct
     9841 taggattgat tttccttggt atcctgttca cgaactccat tggggtaact gtattgactg
     9901 gcctgcttta cctgttcatt tatttcagtt atcaaaaaaa gaatgatgcg ttcgtattga
     9961 cgaatgaatg aaatcgatag aaaattttat tttatttatg tggaatagat ggagctattg
    10021 aagaaaggag ttgtgacaaa agtcttgttg caactccttt ttccgaataa acagtctcat
    10081 gacctcttgg taaacggtgt tcaaaaaccc tctgtgcaaa ataattgatt gttctccatg
    10141 ctcatgacat gatagaagaa aggaatgaaa aaaaggagtt tgttttaaat gaaaaagtat
    10201 tggaagtttt tgttgatgat cggtgtttca acggtagtta tgtttggtat gatgtacttg
    10261 aatgtctatg cgctcgatca tatcttcttc agtcgtacac gagtattcat ggcgttgatg
    10321 atgggtgcag tcatggcagt catcatgctg ttgttcatgt ggaaaatgta tgaaaacaaa
    10381 aagataaatg ttatgatctt agccgctagt cttcttgttt ttgccggttc cttgtttatg
    10441 gtacgaagcc aaactactgt caacgacaca gcttggatga aggccatgat tcctcatcat
    10501 tctatcgcta tcctggcaag taaacgagct gatttaaaag atcctgaagt aaaaaaatta
    10561 gcggaagaaa tcattcaggc acaagaagaa gagatcgaca aaatgaaaaa aatgatcaaa
    10621 gaaatggaat gaccattatc cgtccaaata aaaaaagagg atgttccaga agttatcagc
    10681 ttcaagaaat aagaagaagc tacttctgga acgccgtgaa taaggggttg tgaaaaagct
    10741 gccctgcatc aagtaataag aacagccaaa caaaaatagc ttctcctatt tttcgtttgg
    10801 ttaggcttat taccgcagat gcaagctttt gaacaccgtt tattaagagg ttgtaaaatc
    10861 agcgttctga cctgagtatt agagagacaa atggaaaaat agttcctcat attttgccat
    10921 tttttgagct aataccgagg gtcagctgat tgaacaccgt ttatcgagag gttgtgaaaa
    10981 agtcgcttag tttcgcgtat taagaaaaat tttgtgaaat ggctttccac atttttgtat
    11041 aaatttggct taataccgag aaactggctt ttgaataccg tttattcgta aaagggattg
    11101 tgacaaaagt cttgtcacaa tcccttttta tgtttttatt gttctaattg cgctgtcttg
    11161 cttactgctt cttgcggttt cacaaatttt tttgagattc cccaacctgt gaaggcactg
    11221 accatcgaaa acacaggaga aagcaaactg aaaaatacga atggcaagta agtgagtgta
    11281 ggaataccaa atgtacttgc tacgaaactt ccggcaattc cccaaggcac taagtagttg
    11341 attactgttc caccatcttc tagcgttcta cctaatacag ttggatcaaa tcctgctttt
    11401 tcataagttt ctttaaatgc attaccaggc agaataacag ataggaattg ttcaccaata
    11461 aaaatattta ctccgatact tgtcatcaat gttgtaaaaa tcaaatttgg tgtgttcgtc
    11521 atctttcttg atactcgttc catcacggtt gagatcaagc caaattcgat caacaatcca
    11581 cctaatgaca aagttagaat aatcaatgac acagtaggca tcatactttc gattcctccg
    11641 cggctaagca gtgaatcgat ttgttggttt ccagtatttg aaacaaaccc tgattcgatg
    11701 accccagcca acttcgttaa ggaagtttgt gggttttgaa taaaaatcat tcctactgct
    11761 acagcgatat tcaacaaaat cgttaaaatt gccggcacct ttttccatgc acacagtaac
    11821 attaaaaata aaggaatcag tgaccagatt gaaatagtaa agtttgcttc tagaacatca
    11881 gtcacttcac gaatagatga aagacttgcg gctttacttg tgtgtcctaa aatcatgaat
    11941 aaaaagaaag aaacaacaaa tgccgggaca gttgaccaca tcatatgctt gatgtgtttg
    12001 aacaaatcgg catcaacaat agctgctgct agatttgttg attcggataa tggagacatc
    12061 ttatctccaa aaacggcacc tgaaacaata gctccaacaa ccagcgctgg atttaagcct
    12121 agtgttgtcc caataccgaa aaatgcaatc ccaatcgttg acataacagt aaaggcactc
    12181 cctacagcgc tacctacgat tgcacacaca acaaatacag aagggacaaa ccactggaca
    12241 ctgatcaatt tgaacccaat gaccattaga gttggaatga tccctgcttg tatccataca
    12301 gcaatcaatg aaccgactaa aataaaaata aagattggga tgattcctgg tttgatgcca
    12361 ttgatgattc cttcgtgcag ccaatctgtc gggtatcttt ttatcactgc atacaacatg
    12421 accattccga tcgctgctaa gatcgtgata ttaggtgaga cctttaatcc gacaacgcca
    12481 atcgcaatgc ctaaaacaat aaccattaat acaagtgttg cttctaaaaa acttggtgtt
    12541 cctttttttc ccatgataat ctctcctttt ttagttgttc ttatgttcta aaaaagttaa
    12601 gatatcggat tactccgccg caattcatcc gcgattcctc ctttataaaa ataaaaagac
    12661 ctgttttaaa ctaaggacga atgactccgc ggtaccacct taattaaaaa cagatctgtt
    12721 tttcactttc aaacttttat tcaaatgtat ggactttcca tgtaattcgt caattaggct
    12781 tgatcggttc acagcgacca ccgactttct gaacaaaaca cctaaccgct actgcgtgaa
    12841 aagttgactt gtacgaagtt taccttccat aagatcattt gtcaatgaaa aagaaaatat
    12901 tttttcattt ttctgaaaat tatttttata actttcacac acaagtttat tttttactag
    12961 aaagtagcgc cctcttgttt ttttcttgct actatttctt gatgttcttg cttgattctt
    13021 tctaatagct ttggctcttt caacagatca agagctgtat tggcgagtag ttctgctgca
    13081 atcgcaatag aagcaaggcc tttgtcactt ttagcagcag ctttgaattc ttctgtgtgt
    13141 ccggcaatat atgtctcaga gattgctacc gtaggctgga ttgttggtat gacttggctg
    13201 acatttccta catcagaaga accgagactt gttctttgtt ccgtttctat ttcttcatct
    13261 ggaacacctg cttcttttgc atgctggaag aataagtcat caaaagaagg agtaacgatg
    13321 acatcatcaa ctgcattttg gaacaatcca aactcaaaag tcgtgcccgt acttatcgca
    13381 gcacctcgta cgatattttc cactttttca tatactttat ctaatgtttg ccgatttcct
    13441 gcacgtaaat agaaacgtcc agcagcgtaa tctggaatga cgttggcagc tactcctccg
    13501 tctgtaatga tcccatggat attgacatct tttggcaatt gcaagcgtag agcgttgatt
    13561 ccattaaaca cttggatcaa tgcttccaaa gcatttactc ctttttcagg tgctgctgct
    13621 gcatgggaag cgacaccgta aaatttgatg tcgactggat cattcgctaa agattctgtc
    13681 gttttcccat aacgaaaagc tggatggacg cataacgcta catcgacatc ctcaaaaaaa
    13741 ccttctcgaa caaagctgcc tttagctgat ccgttctcgc ccccttcttc ccctggggtg
    13801 ccatatacac ggatttctcc accagtttca tcaatgaatt gcttcactat gctggcggct
    13861 aatacggagt aagtaccaaa caaattatgc ccgcatgcat gaccaatacc tggtaaagca
    13921 tcgaattctg ccaaaaaggc aattgtggga ccgggtttat tggaaatata acgtgcatca
    13981 aaggctgttc ggtgtccagc tacttctttt tctacccaaa acctttcttt ttctaattgt
    14041 ttaatcaacg ctgctgagga ataaaactca tagttactta cttcaggatg ttcgtggata
    14101 tctagagcaa gtgcttgata ttctggtaaa cgatattgga ttagatcctt caattggttt
    14161 gttgagattg ttgttgtcat aaaaaattcc tcctatgcta gtcatttttc ttattttctc
    14221 ccgaatgttt cccatgcagg cacgcttgat ccttttgatg ttttctcgat tactttgact
    14281 gtgtcttctg tttgataagc gtctactatt ttttgatacg ttttattgtt ttcatcttct
    14341 tttcttgcaa cgatgatatt cacataagga cgtgcatggt catccactgg ttctaagaag
    14401 atggcatcag aagcaggatt aaatccagca tctacagcta caccgctatt gatcactgaa
    14461 atgtctacat ctgacaaagc acgtgctgtt tgggaagcat ctaattcaga aatgtctaga
    14521 ttttttctat tctcggtaat atcagaaaca gtgggcgctt gtccttttgc cttatccact
    14581 ttgatcaatc cagcagtttg caataacaga agagctctgc cgccatttgt aacatcatta
    14641 gggattgcta cttttgctcc gtctttgata tcagctacat ccttcaaagt gttagaataa
    14701 attcccagcg gtgcattgac agtatttccg atcgatacta aatcagttcc gtgttcttta
    14761 ttgtaattgt ctaaaaagaa ttgatgttgg aaagaattca aatcaatcga tccatctgct
    14821 aaagccacat ttggttgatt gtaatctgta aatttcacgt attccaaatt aatgttctcc
    14881 tttttcaaac ggtctttcac gttgtcccat acatctgtat cttctccaat aatccctagt
    14941 tttacgatag tttctttttt gtcagttgta tctgcttttc cttggctaca ccctgccaac
    15001 caaataattg ctacaaatgt aatgactaca aaacgaataa tttttttcat tttttctcct
    15061 ccactttcaa tgtgtaattt ttttaatcag ttgattactg ataaattgac ttaaaaaaac
    15121 taagattaga atcaataatg tagccacaat cgttacatct gtttggaatc gattataccc
    15181 tctcgatata gcaagattcc ctaatccgcc tgcacctact gctccagcca tggtagtcag
    15241 tccgatcacg ttgatgatag tcaacgccga gacccgaatg ataccaggca aaccttctaa
    15301 caaatacaca cgtaaaatga taccgattgg actggttccc atcgcttctg ccgcttcgat
    15361 caccccagga tcaacttcta gcagtgcgtt ctcgatttga cgagcaaaaa acggaacaac
    15421 tccaaaaatc aatggcacta aagctgcttt cgcaccaatc gtcgttccaa ctaagaaacg
    15481 agtagtcaat gctaaaagag aaagcaagat aatgaaaggg atggaacgaa tgatattgat
    15541 cagcttatca agtatttcaa atagcagacg gttagctagt aatccattag ggcgtgtcac
    15601 tactaacgtc acacctaaga tgattccaat catcccgcca atgaatgctg tccaaaaaga
    15661 catatatagt gtttgcttcg tcgcttcgac aaattcatcc ggaatcaaat aaacattggg
    15721 caaatatgtt ttaaagaaat cagtcatttt atcttcctcc ttcgatcact ttgatacgac
    15781 tatctaaatc gtttttttgt aaaatattta tttttacacc agatttgatc aaataatcca
    15841 atgctttttt ccgctgtttg gtttctccag ctaaagtaac gatcaaactc cctaacggcg
    15901 tttcttgtaa taactctaca ttgccatata aaatattcgc tgttacttga tatttgctgt
    15961 acagatgagc gattagaggt tcgttcgtct gatttccaat ataacttaat tccaccagcc
    16021 atttattggc gatctgatcg gcaaaagcag aatgggcaat gatcgtttct aatgcttggt
    16081 caagatgagt agctgttcga ataaaatctt tggtcaattc ttcttcagga ttgctgaaga
    16141 tctggatgct gctacctttt tcaacgattc ttccatcttc cataaccgct accttgttgc
    16201 agatttcttt gacaacttgc atctcatgag tgatcaggac gattgttaaa cctaactggc
    16261 gattcaattt ttttaacaat gcaaggatct gaagggtcgt tttcggatca agtgcactgg
    16321 ttgcttcatc acataataat accttaggtt cattcgccaa agctcgtgcg attgctactc
    16381 gttgtttttg tccccccgac agttgtttag gataagcaga tgctttttct tccaaaccga
    16441 caagttccag tagttcattc actttttgac gacgttcctg tttcgatttt ccagaatact
    16501 ttaaagaaaa atcaacgttg tcaaaaatag ttcggctttc cattagattg aaatgctgaa
    16561 agatcatacc gatggccttt ctttcttttc gcaattcttt gggagagagc tgactcagat
    16621 tcgtctgatt gacaatgact tctcctgctg ttggtttctg cagcaaattc atcacacgaa
    16681 ctaacgtact tttccctgca cctgagtaac cgacgattcc ataaacatcg cctttttcaa
    16741 tcgttaaatc tacagaatct actgcctgta tctctttttc tttttgtttg aaacatacag
    16801 aaacatctcg caattcaatc attaaaattc ctccttatct aaaaaaagcg tcctcaagaa
    16861 aatgaaaatg tcattttctt gaggacgctt ttttgcgtgt taccacctca tgttcaagct
    16921 gtgttcacac acaactcctc ttcaagtacg atcatactct agcaagatat cggtcgcagc
    16981 cgtcatcgcc ttacatctat gctcgggatg aattgctcga tggccatttt ccaatagtcg
    17041 ttccttgctt cttctcagct accgaagctc tctgtcaaag gttcttctat cgtacttacc
    17101 atttcaatgc aaataatatt caaaaaaaaa gtccttttac taacataaac tatgtcagta
    17161 aaaggacgac gtatcgcggt accacctttt ttcggaagtt tccttccctc aagcccaaca
    17221 tctcaaaaag agagattggc gttcgtttta acggtcgaac agttccgaat ttgcctactt
    17281 atcgacaaat tattctccaa ggccattttc agcttttctt acttgactct tctcaccagc
    17341 cagagcctct ctgcaaagtt tccaaactta ctttcctttt caacgaaatc gatattttgt
    17401 tgttgggatt aatctacatg aggaaaactg aaaagtcaag tacgaaattc aaattatttt
    17461 ttctatattt atttttctgt ttttttattt aacatttcaa taacagttcg aagatctttt
    17521 ccttccgcaa gaagtttcaa tttttccttt tcttcgacgg tataatttcg gacaaaactg
    17581 gaatcaggta tcaaaacaac gcctaatcct gctcgtgaag cagctgttgc tcctactaaa
    17641 gaatcttcca ctgcaaaggc tgaactagct tctaatcctg ttcgttccaa agctgtcaga
    17701 taaggaatcg gtgaaggctt gttctccgac acttcgtctc cgaatgtaaa cgtgtccaag
    17761 taatcagcta atttaaagtg atccaagata tctgttgctc ttttcgtaac agtggtcgta
    17821 gcaatgccta gtttcatatg acgttctttt aagtctgcca atatttcttt tgcatatggt
    17881 tttaagtgga tggctccgct tgtcagctgt tgataaaaat attcttcacg cttcgctcgg
    17941 acttcctgta ctttttcagg gctacctgct gctttcacta aaatatcaaa actttgtttc
    18001 cagttttttc cgccccaagc gtcaacgatt tcttttggga tatggatatc aaattgctga
    18061 aatccccaaa gccagccatc acgatacgtt ttttctgtgt caactaacaa accatctaaa
    18121 tcaaatattg cacctttcat ctatacatta atcctttcca aaacaataca catagtttag
    18181 cacgactggt ccaaaaagaa cagaagcaaa gaaccagcct tctcgtttat aagagttctt
    18241 ggaatcgacg gatatagatt ttcttcacag ctgtcacaag cgccagataa gcgatgactg
    18301 tagcaaataa ccataaccag taattgccag gaagtggcat caagcctaat tcagcaccaa
    18361 aacttgtaaa aggaaggaca gaaccgacaa taattcctaa tgtagtgata acaaacatgg
    18421 caaatgttgc gttgctttgg ataaatggta ctttcggtgt acgcagtgca tggagcacta
    18481 aggtttgtgt ccatagtgat tcgacaaacc atcctgcatg gaataatgcg atgaaaatca
    18541 ctcgctgtga actatccaat gtatggaaac ttccgcccac tacagcagga caaataacaa
    18601 aatacatcaa caagtaagta gtaatatcaa atatcgaact tgttggacca aagtaaacca
    18661 tgaattcccc aatactagat gcattccatt tttttggctc gtccaaatat tctttatcca
    18721 tccgatccca tggcaaggaa atgcaggaaa catcatagat cagattcaaa aacaaaagtt
    18781 gtaaaggcag cattggtaaa aatggcagga acgtacttgc aatcagcacg gaaaacatat
    18841 tgccaaaatt agaacttgct gtagccttga tatatttcat gatattccca aaagtttctc
    18901 gtccagaaag aaggcctctt tccaaaatcg tcaaatcttt ttctaaaagg atcacgtctg
    18961 cagattcttt cgcaatatcc acagctgtat ctacagaaat accgatatca gccactttca
    19021 ttgcaggagc gtcattgata ccatctccta aaaatccgac agtatgacca tttttacgta
    19081 aagctgtcgt caaacgtgct ttttgctgag gattcaattt tacgaatact tcatggtctt
    19141 cagctacttg tgctaattca tgatcactca tttccgagat tttctcaccg gtaaccaatt
    19201 cattgatctc tagacctact tgacggcaca ctgaacgtgt aactaattcg ttgtccccag
    19261 tcagaacttt tacagctaca ccatgttctt tcagagcttt caacgcttgt ttcgttgttt
    19321 cttttggtgg atcaaggaaa gcaagataac caatcaaaac catgtctgac tcatctttga
    19381 ctgaaaattc tccaacgacg cttggattgg tcttttgtgc gacaccaatc actcgtaagc
    19441 catcttcatt aagtgactgt acttttgcaa gtacggcttc ttttcgttct tttgttaatg
    19501 gtgtaacagt accgccaaga tcaataaatt tagaaatgtt cagcatttct tcaatcgcgc
    19561 cttttgtgat catttgtgtt ttaccagctg gatcagcaac cacaacactc atacgacgac
    19621 gttcaaaatc aaaaggaatt tcatccactt tttgataatt gatcttatct gtatctaaag
    19681 tttctgttgc ttcatcaatg atagccacat ctaacaaatt tttcagccct gtttgatagt
    19741 agctatttag atacgcatgg cgcaaaacac gatcatcttc atttccttca caatccatat
    19801 gatattcaag aatgatttta tcttgtgtca atgtccccgt tttatccgta cataacacat
    19861 cgattgcacc aaagttctgg atggcattca aatttttgat cactgttcct tttttggcca
    19921 tcacacttgc tcctttgacc aaattcgtcg taacgatcat tggaagcatt tcaggtgtta
    19981 gaccaactgc tacagatagt ccaaaaagaa aagcttctag ccaatcccct tttgtaagcc
    20041 cattgatgac gataactgct ggagccatca atgacataaa tttgatcagt agcatggatg
    20101 ttttatggat gcctaattca aagcttgatt gaattggttt ttcagatagc gtttctgcta
    20161 catgaccaaa tagtgtttca tttccaacag aaacaatgat tccttccgca cttccactga
    20221 tgacattact tcccataaag acaagatttt cgtaacttgt ttcgctttgc tgatcgtctt
    20281 cacatacttt cgcatgcttt tctactggat agctttcccc tgtcaatgct gcttgggaaa
    20341 tgaataaatc ctttgcggac aataaacgca tatccgctgg tatcatatcc cctgctgaaa
    20401 gccgaacaag atctccacag acgatttctt ccataggaat ctctgtgtac tttccattac
    20461 gttttacagc tgccgttact ttcacaagtg atttcagttt ttcagccgct tgattggaac
    20521 gtacggactg aatcaacgtc attgtcccgc tgacgataac catagtgaag ataatggcta
    20581 caccgaaaag atctttatct ccaggagctg ctaatacata ttccgtgatg aatgaaataa
    20641 ttcctaatgc gatcaacact aatgtaaatg gcgtgatgta tgctttcaat atttctacaa
    20701 tgaatggtgt ttttttgcca tatgaaatcg cattttcacc gtaattttct cttgaacgtt
    20761 cgatttgttc ttctgtcaat ccatttgctg atgcatggaa tagttcaaat aattcttgtt
    20821 tcgtttttcc agcaaaagct gcatagattt tttgtgtatc tttctttttt tccatctcaa
    20881 tctctccttt gtgtccaaaa gacagctgtt ctcgctgctt ttggtcttta gctattcatc
    20941 ggatcgatag agaaaaaact gaaaggggct gagaacagat agataatagc cctggccttt
    21001 cctctagatc cttgtttttc gaatgactgt catccatttg ttctcatctc ctttactaag
    21061 taattttaat tcatgacttc caatcatttt tcgaatagtt atttttccaa ttagaccacc
    21121 tcctttttat ttttcaccga tggggatttg cggcaacaaa aaaacctctc gccaaatact
    21181 cagcgaaagg tttgaaaaag catattaaaa aaagcttctc agatctcatc gttgagtttt
    21241 gacactgtac aacgtaaaaa gcagatacct gcttttagct accttatgaa aagccttatc
    21301 tcgacaattc ctgttctacc cattggcgtc tctcgacgtt tttgggcagt agcctatgtt
    21361 tgtataggag cctcacctaa caaatcttat tcggttttcc ttcgttaata taacaactaa
    21421 aaaagaggat gtcaaatcaa ttttccatgt ttttcatact ttttttaaaa tacacacgtt
    21481 atattcctgt ctcacgcggt tttatttgtc tcattttcca cctcccgcta taatagaaat
    21541 atagtaggaa acgaggtgct gttatggaaa gaacacttgt tattatcaag ccagacggcg
    21601 tgagacgcca tttagtcgga agcatcatcc aacgttttga agcaaaggga ctggccattg
    21661 ctgaaatgaa gtttgaaact atgactcctg aattagcaaa agaacattat cagcacttga
    21721 ctgagcgcag tttttttgat gaactgatcg attacatgac ttccggtcct gtcgtttatc
    21781 ttgtgctggt cggcgaggaa gtgatcgata tcgtccgtaa gatggttgga gcaacaaaag
    21841 ctgccgatgc tgttccaggt acgatccgag gagactacgc gctgccgggg actgaaaaca
    21901 tcattcacgc ttctgattcc cgtgatgctg cggtaaaaga gatcgcccgc tttttcccgc
    21961 cattcgatat aaacaaaccg tctgaaaaag cggcgaagtg atacaaattc taaactgctg
    22021 gtctagttgc tgggttcagc aatggccagc agttttttct ctaaggacct ccgttgattc
    22081 tttcctaaca tatattcgag cactgtgaag aattcttggt agtctttcgt cttttcttcc
    22141 cctaaagcag cagaaatcaa tctttgataa taatctgcca tcaatgtttc atagatcact
    22201 gtacttcttt ctttatgtgc taagctccgt ttcatatctt cattcacact acgtgccttt
    22261 ttcaaccgac cattttctat ataaaacgaa gtcaggttca ttaaaaaagt aaagagatac
    22321 tgctcatgat tgttcaactg tgctaatcgt cgcattcttt ttttgatttt caaataattg
    22381 ttctcaatga accgatcact gaatatccaa ggaaaatcag ctagaataga tagttcaaat
    22441 tgaccatagt ctggtatctg catcaagtat tcttcaatga ttcggcattc ataacgtaaa
    22501 aattcaaatt ctgtctcaga tacttctgtt ttttcgacca tgtgaagatt caaaaccaaa
    22561 taatacagat cttctgtttt cttaaaatgc ctacggttat tgctgatctc tttataagga
    22621 tcaaagctgt cttcttgtac ccgatctagc attcgtcttt ttttctttaa ctttattggt
    22681 tcatccagct ggtttcgcat ccattcatat tctgcaagtg tcatgttcat tcgctctaaa
    22741 aatgatctca atgttacaaa tgacattttt ttcactgaat ttgtctcaat cccgactaga
    22801 gaagtcttaa aacagattcc ttccgctaat tgggactgtg tcaacttctt gttgactcga
    22861 agtttcttga tcaattcacc atcatacata atagttctcc tttttaattt aaatttttcc
    22921 acattcaata aatagatacg ttaaaaagga aaaatacacg aaaaaaataa aaaaatgttt
    22981 ttttcataaa aaataccaac cttcaagcat tagaatgttg tctaactact gaaggttggt
    23041 ttgattgttt attgattatt tttttgctgt tcaacgtctg ttcttgttac tggatataag
    23101 ttggttgttg ttttattacc attttttgaa aagacacttg tcgaatgttc gcctttttgc
    23161 ttttcgattg cttcattacg ttccgcttca tttttatagt catatttttt cggatcaatc
    23221 gtctcaaatc cgtttggcac ataaaaccgc aagagatttt cctgattcag cttatctgaa
    23281 agacttaacg cagtcttgac tttttcctga tctttggaga tttcctcatc gattcctgct
    23341 gctttcgtat cgatgatttc tcctgtctga ttattgtaag cttttcctcc taggacggtg
    23401 tatttgggtg tcacaaagtt cccatttcga aaagcaacta cttgatcatg ctgagaagat
    23461 aataagtctg taccaaaatg aagataatct ttatcatcga ttcccaatag atgtagtaat
    23521 gtcggcaaaa catcgatttc tccaccgtat tcatgattga ctgtcccttt agtataacca
    23581 ggcatatgga tcatcaatgg cactcgttgc atctgtgcgt tgtcaaattc atcccatgta
    23641 tctggatctt taccaagagc gcttgctaaa tctttattat ctgtattcga gatcccaaaa
    23701 tgatccccgt aaatcacaaa gattgaattt tggtaaatac cagatttttt caaatacgta
    23761 aaaaattgtt ccaatgattg atccagataa tgtgccgttc gaacataatt atctactgtg
    23821 ctattgccag tattcaaaga tggaaagtcg aaattcttgt catccgtata ataaggcgtg
    23881 tggttggtta cggaaagaaa tttcgtgtaa aacggctgtt gtagatgctc aagatattgg
    23941 gcagattctc tgaataaatc tttatctaat atcccataac ctaacgtttc gtcggattca
    24001 tcaaaataag atcgatcaaa gaaattgtca taacctaagt ttttatatac atgatctcta
    24061 ttccaaaaac tagctacatt cccatggaaa acagcgcttg tatagccttg cttttgtcca
    24121 aggatcgctg gtgcggcttg aaagacattg tctgacccaa gttctgtgaa taatgagcct
    24181 tgaggtaaac caaatgtgcc agtttccaac atattttcag catcacttgt ctttccctgt
    24241 cctacttcat gaaagaaatt gtcaaagctg atcgttgctt gattctggaa aatactattt
    24301 aaaaacggtg tgacttcttg tccgtctacc ttcatattaa tcaaaaactg ctggaaactt
    24361 tctaaatgga gaacgatcac atttttccct ttagcgatcc caaatgtttc gggatttggc
    24421 tctgcataat gtttctttgt ataatctaaa acttcatcaa tcccattact gcttgcatgc
    24481 gcgcgaacac tgctagtcat tcctgttttg attccgtcat aaatagtata cgcatcgatt
    24541 cctagatatt tgacaatata tgaacggtca aatgttcgct gcagcaattg tggacggttg
    24601 gcttcgctca gcgctagatt tcctgaaaaa ataagccctg aaagacaggt aacagcgata
    24661 gccatcggtt tgccgacagg gttgcttttg ataggtattt tcttgacctt caaccaaacc
    24721 agtagcccaa tgaacacagc gatatccagc cagtagatga catcatgggg cttcatcaaa
    24781 gaaaaggagc ttcctgacaa tccttgcgac actttcgaaa agcctaatac agatttgact
    24841 gtaatgaaat ctgtgaattc ccgataaaaa ataatattca agtacaacaa aagcgtattt
    24901 aaaatatcaa tgatcaaaag aatagctaac gctggctttg gctttttaat atacaaagaa
    24961 aggccaaaaa acaataaggt agtcgcaatt gggttgatcc acaagataaa gtactggatc
    25021 gtaccttcca cacctaatga aaaatctgca tagtaggcaa tgatcgtctt cagccagaaa
    25081 aagaaaacag ctgtcaaaac gattgctaaa cgattttggc acaatttttt aagttgtgtc
    25141 acgtatatca cttctctcta ttcacatttt ttcttatttt gctcctgaaa aatgaaaatt
    25201 tcctaaaaaa gttgttatct taagattttt ttattctttc taaagattat tccgtccact
    25261 tttcacagca taaaaccgct gcgatgtaag cgattaaact aatgaagaga taaaagtctt
    25321 gtggtgaccg cttcactcca cggtattcaa aagccagttt ctcggtattc gtacaaaaaa
    25381 agaggctgtg acaaaattct tgtcgcagct ccttatttct tgatgcagga cagctttttc
    25441 acaacttctt ccttaaacgg tgttctcctg ccaactcctc ggccacaagt cacaatattt
    25501 ataaaacatg agaagctgtt ttttgaaatt gttccttgtg gctcagagtt aaacggcagg
    25561 ctcaagacct cttgatacac ggtgttcaat cagctgaccc tcggtattag attatgtcag
    25621 gacgctaatt ttacaacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa
    25681 gcccaaccaa acgaaaaata taaggagcta ttttttgaaa ttgctccttg tggctcagag
    25741 ttaaacggca ggctcaagac ctcttgatac acggtgttca atcagctgac cttcggtatt
    25801 agctcaaaaa acggcaaaat atgaggaact attttgccgt ttgtttctct aatactcagg
    25861 tcaaaacgct gatttcacaa cctctgtttt tcattgtcca taggattgat attgtccttg
    25921 tgatgactca ttcgtaataa ttgaggcttg attttcttca cttcccgtta tcgtttgtcc
    25981 actgccagaa tctgaaggac ttgttcctgt cgactcgttt ttttgagaag agacagttgt
    26041 tttcttgact tgttcatttg tctttttcca ttccgctaaa cgatcggcga ccactttttt
    26101 gtctactttt tcagaataag cggtgatcac ttttccattt ttcaaacggg ctactttgtc
    26161 gccatcatga gtcaaaagca aaacggtcaa atgcttgtct ttaaattgat attgtctgag
    26221 agaaatcgca ctaagtacgt catcgatatt aataaattga ttttcttctt ttccttttgg
    26281 caaataaatg tccaattcgc ctttttcgta attggcagtt attttatact ccgcccacca
    26341 ataagttgag aattcttttt tgaaattatc tactgattct ttgcttgcta cgattttttc
    26401 tgtttctttc tgagcaagat ctgctgtcat ttttcctgaa aatgctgcgg ataaaataag
    26461 cagtaagaaa aaaccgaata accaaaagag aagaaacggt ctttttttcc tcatgctttc
    26521 ctccactcct atttaatttt gactattcta ttatatacaa aaaatgaaat agaaagacaa
    26581 aaaagttgaa aaagatcgta aataattgaa cttctttttc acaaaatggt aagaatggag
    26641 aatttcacgc atttgctctc aactattctt tcttaagcct caaatccgtt tttatctgat
    26701 acgtctttga accaatgacc cgattttttg attgttcttt ttccatcttg tgctaagtct
    26761 actgaaataa agccatatcg attcttatat gcattggtcc acgaccagtt atccatacat
    26821 gtccacatat gatagcctaa acaattggca ccttcttgaa tggcttgatg gacatatttc
    26881 agatgagatt ggacaaattc gatccgataa tcatcgtgga tcatgccatc ttctttgata
    26941 aatcgtgctt cgttttctac acccataccg ttttcagaaa taaagcagtc gatatttcca
    27001 taatgatctc gtacattgat caaaatatca tagattcctt gttcatagat ctcccatccg
    27061 cgataaggat tcattttacg accaggcatt tcataattgt cgaaataatc ctcaggcatt
    27121 ggaccgtttt tagtatcgat tggtgtttct tttgctttta cccggcgtgg ctggtaataa
    27181 ttgataccta aaagatctac tgtattttct ttgataaccg tcaaatcctc tgcatggatc
    27241 acaggcatga gatcaagttc ttttactatc tcaatcaatt cacttggaaa ctcgcctttt
    27301 acagctggat caaggaatga acgattgaag aacgcatctg ctatttgcgc tgctttaaca
    27361 tcttcagggt ttgattcgtc tcttggatag cttggtgtca gattcaagat gataccaata
    27421 acaccacctt gctccatttc atggtaagat ttgatggcta acgctgaagc caacgcttca
    27481 tgatagccaa cttggatcgc ttttttcata tctgactctt ctggataatg ccatcgatat
    27541 aaataacctg cttcgactgg aacgattggt tcattatgag taaaccattt ttttactttc
    27601 gaaccaaaca attcaaaaca acttttggca tatttttcat aggcttcaac cgtttctcgg
    27661 ttcaaccatc cgcctttttc ttgcagatac atcggcatat cgaaatgata cagattcaca
    27721 aagggttcga taccgttttc aatcaactca tcaaaataac gatgatagaa acgaacggct
    27781 tcttcgttga ttgttccaga ttcatcaggt atcaaacggc tccattggat agatgttcga
    27841 aatgaattat gcccagtctg tttcatcaac tgtacatcct cttgatactt cgtataaact
    27901 tgcgaggttt tttcaggtcc tacttgatca aagaactttt gcggttcttt tttgtaccaa
    27961 taatcaaaaa tgttctctcc ttttccatcg cctgaaatcc ttccttctgt ttgtgggcca
    28021 cttgctgcag aaccccacca aaaatttgct ggaaattgat attttttcat caaagcacct
    28081 ctttttcttt tttagttcgt tttagttgat tccatgtatg atattttcca tcacaatcac
    28141 tttttcattt tcttcctctt cttttttgat tcgttttaac agctgttctg ctgctcgttc
    28201 cccgatttta tctgttggtg gcgtgatcgt gattttcgga aggatactca gatcgttttt
    28261 atctaaataa tcatagtgac tgaacgcaat atccttcccg attctgacac cttcttgatt
    28321 gaaacattcg atggcaccaa tcgacatatt gtaattgctg acaaaaatag cagttgcccc
    28381 tttttctaat aatgcttttg ccccgtaata gccgcttttt ctagaatagt ctcctgtaaa
    28441 gacctctaat tggatcgatg gatcattttg gaccgcttct ttggctcctg ctaaacgttc
    28501 tcttgctacg tagtctgttt gagtggcagc aataaatccg atttttttat gtccttgttc
    28561 accatgatga gcgatcactt ttgctacact ttctcgatca ttcactaata ttgtatcaat
    28621 acctgctggt ccttctggac aattcaatgc gataactgga atactcattt ttgtgagcat
    28681 ttcaaatcca gaccactctt cttcggataa gaagacaatc agcccatcga tcacatgctt
    28741 catcaggtat tcaatttttt gttcaatttt ttctggctga tcggcaaacc cgcttaataa
    28801 taagctatac ccattttctt cagctactct ttcgatccct gagacaactt ccgacccaaa
    28861 acggctggaa atcccgttta tcaaaagtcc aatagagaag cttcgattat tcttcaaccc
    28921 tttagcaatg atgttttctt tatagtccaa cgctgaaatc gcttctgcta tttttttcat
    28981 gttcttttcc ttcagttgct gaccattcaa gtaacgggaa actgttccca ctgaaacacc
    29041 agccatctta gccacatcac ggatcgatgc catacattct cactcctgcc agttacattt
    29101 gatttctctt tcttgttgtt cgattatgat gaccgcattc tttgcatagt gttgcttgtc
    29161 atcaatatcc atataactaa agtcttcagt agagcaactt cctctcacta ttaattatac
    29221 ttaaacaagt atttgaaccg gttcattttt aatatagcat ttttttaaaa gattgcaaca
    29281 cttttttaaa acgctttctg aaagtggcgg attttttgcc tttttaaaat tttattctaa
    29341 aatttactct atttgtgggt gttttgtgcg tttttctctt ccatccgttt gaagttttac
    29401 attccaatgg tataatttct ttatcctatc aaatggggga agtattttga agaaattaat
    29461 cgtactattt attttgtctg cgggactttt gacattgact ggctgtgatt tgtttggcgg
    29521 ctctgaccaa tcctcggacg aaacaaccga ttcttctgtc ttaaaagcgg aaaaagcaga
    29581 taccaccaac agtgtatata cgctaaaagt acgtaaagat atggatttat ccgcgggaca
    29641 gttgacattt acagataaaa aaatggaatg gacacgcacg tatcagacac aaggaactga
    29701 ttcttctgaa tcatctgaag aaaaaacagt gctgacagat atcaagatca ctaccaaaga
    29761 tgacgaatat attatttcag gaaaagaaaa taaaaaagaa acaacagtca tatttacaaa
    29821 aatcggtaat taccgtttga aggatgagga tggcaacatc tacagtctat agtgccgtct
    29881 gttggatctt cttttgtttc tccccttctt ttctgctata gtaaagaagc aagatgattg
    29941 taaaggagac gaaatggatg atcgatagtc ctgattattg tacatgtaat aacaacaaaa
    30001 atatccatac agaatttaat gaccaaggaa gcttgagcat atgcgacaac tgtcacaaac
    30061 tagtcaaaga ttcatttaaa acatttgaat cttatcctgt tcaaaattat gtaaaaagta
    30121 gaagaagata aacaaatcca agcaaaactc actatggctg atttttatga acttcaaact
    30181 cagcttcttc ctttgatcaa gcttcatcag taaaacaaac agtggatcat ttcagtttac
    30241 aaatcaaaga gtaaagattt atttatttca aatatttgaa tataaaataa ccaaacgata
    30301 gaaaggctta ttctacatca cgtagtaatt tttctttccc gtcgtttggt tttttatttt
    30361 tagcctaatt cttgaccttt tgcatgctgg ttatccgtaa aatattttcc agcaggcttt
    30421 ccttccaatt cagctaattt ttcttggatc tgacggatca ccttttttgc atcttctgga
    30481 ttttctacaa aatcgaattt atccccatcc acttgtattt taggacatac atcaaaatct
    30541 tcataccatt gattatagcg ggtgttcaac gtttcataat aggtatataa cgtctcatcg
    30601 ctttctagct gttcatagtc acgcccacgt tttttgatac gttctagcat cgtgtccaaa
    30661 gaaactttga tatggaccat caaatcaggc cgtttttttg gagcaacatc atctaactcg
    30721 tttagcatcg tatctaataa gctatcgtac tgctgtactt cgatatccgt tacccgacct
    30781 aaatcagcgt ttagatggaa caacaggcta tcttcataga tggatcggtc tagcacattg
    30841 tcatcgttca ctaaagcatt tttcattgct aaaaatcgct tgttcaaaaa gaagatttgc
    30901 aataaaaaag cgtactgctc aggatttgaa taaaagagcg gcaatacttc gttatcttcg
    30961 acattttcat aaaatggttt agaatcaatc tcttttgcaa gcatatccgt caaactgctc
    31021 ttacctgcac caatcgttcc tgaaatcaaa atcactgaca tacaaccctt cctcttctat
    31081 aagtactcgc ctacgtaagt ttcaacaggc aatgtttcaa aatcatattc acgtacagct
    31141 tggtctgatt ttaaatacgt atggagactt tgtgtaatca tcaagttcgt aggtaccgtc
    31201 tcttcttgta caatgatcat cggttttttc aacatagttg ccacacctaa ttcgtaagcc
    31261 gttcctggat cgattgtttg atgctcaaag tcaaggatag caacaacaaa ttctgcattt
    31321 gtcagttctt ccatatcttt ttcgtatact tcctgcgccc atccagcgct gaatagttca
    31381 taattgcttt cttggtgatg acgtggagag tagaaacttg tgactgtttt gttttcctcc
    31441 aacgcttttt cgatacgact tactcgatcg atttgctcct ctgaaaaaaa tggtcctgct
    31501 aaatatattt gactcattat tttgcctcct tcaataataa ctgattttta atagatactg
    31561 ctcgtaattc attgaccagt tccaatggat tgttggaaaa gattacttga tccaaccatt
    31621 ttttatccat aaaatcctct tttgcaaaag tttctaataa ttgttgcaag ccgctgtaat
    31681 aaccgccaat attcaatatt ccgattggct tgtcgggcgt cagaccgatt gctatccaag
    31741 aaagaagttg tgagaattcc tccaatgtac ctagacctcc agggaaaacg aggacagcat
    31801 ctcccttttc aaataataat acctttcttt catcgatcgt ttctgtagga ataaatgtag
    31861 taacatcttt tccaggcaat tcagtttcaa aaagcccatt cggatagata cctgtgactt
    31921 ttccgttatt ttccagtact gttttactaa tcactcccat aatcccggag tatgaaccac
    31981 cgtaaaccag ttcgatttct tcttgtgcca tgtattttcc taacgtttgc gcaataaatt
    32041 tgtaactttc tttattacca aaacgggaac cgcaaaaaac agtaagtttc ataaaaaaac
    32101 actccctaac tcaaaagtct atcttcattc tacacgtgtt tttttaaaat aaaagactag
    32161 attttgtagt tttttagtgg tatataggat agacgccact atatatagta tttagaaaaa
    32221 aatcattttt cagccatttt atagataaaa caaaaataaa taacgtgttt tcatcgatat
    32281 gaaatgactt acttatagaa gatactggga tttcgtccta tatgccaata acgtgtatta
    32341 ttttaaacaa caaatacttg tttcttttat taacgaaaaa atcattttgt tctcgtgtta
    32401 tgtttcactg ataaatgatg tttctctaaa taaaaaagcc gacgaactaa acgatcaaaa
    32461 tttgatcatt tttgttcatc agcttctcct aaaaatcttt ttgttaacga taaatagctg
    32521 gatttgtaaa aatatcactt gaatcgtcac tgctggaaga aaattcagtg ataaccgcac
    32581 ctgttgcgcc tgcctggaac tagtgtttga tattgggctc gaacgtgtac tgatctctag
    32641 catgaagaat aatttctttt tttactgtat aataactttc tttccttttt ggaatagtac
    32701 aggaaagttt ttttgtttcc tttccttcga cataaggtat accagaccct ttcgacaacg
    32761 aaaggttctc atttttcctt catcgtcatt tcaaggaggg tgccgatgct ctggacagtt
    32821 tcgatcgctc ctaaaccaaa atctgtatat tcgattcttt ctaactctga ttctttcata
    32881 ccattcctgt ctcaaggtac atctattgta cttttttctt gtaatccatt ctgcctctcc
    32941 taactaattg tcaaatcatc ccaagatatg tcttcttcct ctttttcttc ttggatgatt
    33001 tctgcattcg gatctactgg ttttttcaat aataaaatgg ttattcctgt gacaaaagaa
    33061 ccagctaaca atgcaccaat gaatgcccat ggtctggtca tcgtagcgac tgcaaacatt
    33121 cccgatgccg gaatacggct atctgcgccc caaaacatgc ttaatccgcc acctactgtc
    33181 ccaccgactc ccgttgctat gacattgcgt gccaagtcgt tcaatacgat agggaaacat
    33241 ccctctgtga tctgacagat ccccatagga aaagcggtct tcaatgtatc gatttcttga
    33301 cgattgagta tatttttacg aatgattttc cctaagaaat aagcgatcgt caagccgaat
    33361 ggggttacca tcgaagctaa aatcaataca gtgattggtt catagattcc ttctgaaaat
    33421 cgtgccacac ccccttatcc agaataaatg gtaatccaga aacagccgtg tatctgcggt
    33481 aataagccta accaaacgaa aaatatgaga agctattttt gtttggctgt tcttattact
    33541 tgatgcaggg cagctttttc acaacctctt tttttattta tgtttcataa tcaatatggt
    33601 aaggatgcag ctccgtatgg tagaccgcct ccgaatattc aatcatttcc ccaatttgcg
    33661 tagacgattt ccgtatccgt ttgatgccga tctgtgcgtc tgttttcaga attccttgtt
    33721 cttcttctgt taaaaatact gccgagaaat catcagaaaa tgagtcaata gtatatcctt
    33781 ttttcgtcag caaacgatac aaagattctt tgccaaattc ttctctgctg aattgcatca
    33841 tttcttgtgg aagataatga acaaaataaa tataaggctg attgttcaaa taatataagc
    33901 gcttaaaaca aacagcttgc tcaccgaaaa aggaaaacaa aggatcttcc ttctttatat
    33961 ggaccaattg caaatccagc gtttctttcc ggacttcata tcgggagttc tccaagattt
    34021 gcgtaaagct tgccgctttg gataatcgat tgtaaggacg attgcttaga accgtcgtcc
    34081 cacgaccact tttctttgcg acatattcat ctgatgccag cagttcgatt gcctttcgga
    34141 cagtgatttt gctgacttga aacagttctt ctaattcact ttctgtcggc aacatcgaac
    34201 cgactggata ttttcctgag aaaatatctt ctttaatttt atctgcgact tccaagtata
    34261 gtacacgtgt ccttttcata acgtcaccct ttgctttact attactaaaa gaatagcaca
    34321 taaaaaataa aaaaagaaca caaatcacat gtgagaagtg tgttcttttt cattcagctg
    34381 ctttaattac aagtttacgc catttgaggc aattacttct ttataccaat caaaagattt
    34441 tttctttgat cgattcagtg tcccatttcc ttcatcgtct agatccacat agataaagcc
    34501 gtaacgttta ctcatttctc ctgttgatgc actgacaaga tcgatacatc cccaaggcgt
    34561 atagcccatc aaatctacac cgtctgctac tgcattagcc atcgcttcga tatgacgacg
    34621 taaataatcg atccgataat catcaataat cgtgccatcc tcttccatat gatcaatggc
    34681 tccaagaccg ttttcaacaa tgaataatgg tttctggtaa cgattgtata attcattcaa
    34741 tgcgattctc agcccttctg gatcgatctg ccagccccat tcacttgcct caagaaacgg
    34801 attttttact cctcccagca agttcccttc tgaagttgct gcttgtggat cagtctcatt
    34861 gattgccgta gacatataat aactaaatcc gatgtagtcc actggatatt cttttagcaa
    34921 ctggaagtct ttttccgttt gctctaattt ttccggatcg actccatatt ttttatgggt
    34981 acgcgctgtg tatgcagggt attctccacg tacttggaca tccgtgcaga aaaaattgaa
    35041 ttcttggttt tggatcattg ttgctgcctg attgactgga ttggcatcga tgctgtaagt
    35101 tgttgcatag ataatcatgc agccgatttg gatatccgaa cgcaattcgt ggccgatttt
    35161 tacggctttg ctacttgcaa cgaattgatt atgccatgct tggaaaatat tttggtactc
    35221 tcctgctcca ttgcttttta ctaatccttg acttaaagct ggaaagtgaa aggccgaatt
    35281 gatttcattg aaagtcatcc aatatttgac tttactgctg tatctctcta atactgtctg
    35341 agcaaatcgt tcatagaagt cgatcagttt tcgatttttc cagccaccgt attcttttgc
    35401 caaatgaaga ggcatttcgt aatgagaaat cgtgatcaca ggttcaatat catattttaa
    35461 gcactcatca atcagctgat cataaaattc taatcccgct tcgttaggag taccttcgtc
    35521 tccatttgga aaaattcgtg tccaggcaat cgaaaaacga taacatttga atcccatttc
    35581 cgcaaacaag gcaagatctt ctttgaaacg atcgtaatga tcgattcctc gatgattcgg
    35641 gtaaatatat ttttcttgat caatcgtcca gtcaaattct tcactgccga taatcgcaaa
    35701 gcgctgctta cctccaggca tagcatctgc aacggatagt ccttttccgt ctcgatcata
    35761 agccccttct aattgatttg gcagcagttg ctccacccca taaaaatttt tctgaaaaac
    35821 gtgattgcat cctgatttcc tccttatttt acaattgcca gaacatcttc gtttgcaata
    35881 atctcttttt ggtctaattc aagtatatcg gcaaatgcat cagtattcgt aatgacaact
    35941 ggagtgatta cgctatagcc agctgctcta attgcttcga tgtcaaaacg aatcaataaa
    36001 tctcctgctt tgacgagatc cccttgtttg actaagatct caaagccatt cccatttaat
    36061 tctactgtat ccatcccgat atgaatcaaa atctccgctc cgtcagtcgt tgtgatccct
    36121 acagcatgtc cagttggaaa aatagtcgtg atctctccgt ctgcaggtgc aactagttct
    36181 cctacttctg gatcaatagc gatacctttt cccatcacgc cagaagaaaa aacttggtct
    36241 ggtacttcac tcaatggcag tactttaccc gttaaaggac tttttaaggt atgccgtgct
    36301 gaagtaatcg gttttcctgc attcgcatgt ttgttttcta attgattgtc ttgtgtttgt
    36361 tcgtcaaatc ctaaaatcaa tgtacctacg aatgcaagaa caaaagcaat tcctgtagct
    36421 attactgcta ctgtcacatt tgactctaca ccatccaccg tattaatgaa agtcggaatc
    36481 gtcaataagc tgaccaaact gctggagaaa gcttttactc cggaaaatcc gataatagct
    36541 ccaccaatac ctccagaaat acaagcggca atgaatggtt tcttcaaagg taatgtcaca
    36601 ccatacacag ttggttcagt gattccaaat aacgaagtca ctgtagaaga aatccctaat
    36661 gctcgaagtt tcgtatcttt tgttcgcaat gctacagcta aagcagcccc accttgtgcc
    36721 aagatggctg gaagaagcat tggcatcaat acatcaaacc catattgttc gatattcaaa
    36781 aacatgatcg gtacgaatcc ccaatgcatg ccgaacatca cgaatacttg ccataaactt
    36841 cccatgatta gacctgctac gattggacta aatccataaa tcgagttgaa gagaccgcct
    36901 aaagcattac cggcaaccgt cccgattggt cctaaagcaa gaaatgttaa tggaaccatc
    36961 actactaaaa cgacaagcgg aactaaaatc atttgcagaa attgcggaat cacttttttt
    37021 acgaatggct ctaatttact ttgaacccat acggctagaa tgattggtat gacagaagaa
    37081 gtataaccag atggtgaaag aatcactggg atcccaaaga aactgatagc ttctcccgca
    37141 ccagcaactg ctgtgatagc tggatacaca agtgccatag caattgctac tgctaaaaac
    37201 tggttggctt tgaacttttt agctgctgta aaagcaagca tgacaggtaa gaaggtaaag
    37261 acaccatctg ctgcagcaaa caatatttga taagtaccgc tatcagctgt cagccagccc
    37321 atcaccgttg ccaatgataa gaatcctttc aataccccgg cggctgccat cgcccctaga
    37381 aaaggtgtaa atatactaga gataatatcg atcagacgat tcaaaatatt tttggactct
    37441 tcttttccat cctccgaaac tgcaagacct tcctcatcca cgatcgattg ataaacatct
    37501 gctacattac tgccaatcac tacttgatac tgaccgccac tttgtacgac ttggatcacg
    37561 tccgggtctt cttttaactt ttgcgtatct gctaaagatt catcttttaa tctgaatcgc
    37621 aaccgcgtag cacaatgtac taaacttttg acattctttt gtcctcctac cgcttcccat
    37681 acacgacgac caactgcttg attatccatt gttttttcct tctttctttt ttatttaaaa
    37741 aataaaaacc cagattgaaa aaaacagtca caaacattcg tgaacctatt ttttcaatcc
    37801 aggttttgcc tgccatacag taacaatcct tatgattgga tgcttttttt ctcacttact
    37861 actctttgaa tatgaatcgt taaataaatc tgctctgcga tccccatttc ctgtttttgc
    37921 gtttgtcgaa gatactcatt gatccgttcg acacaatgaa acgcttttgg atactttcct
    37981 tgaactaatg cgtagagaaa ttcatcttgc tcgtcagatc cttccccttt gagataacgc
    38041 tgggcaaaaa actgcagatg agtaatgata cgctgataat tcaaagattc ttcatcgaac
    38101 acgatgccga agaatttact gatgatactc agtatatctc ttaccattgt ggtagcggtc
    38161 atcgtcactt gcatgtcttc gtttccttgt tggctattca caaaatgaaa ggcaataaaa
    38221 ccggcttcat cctcagaaag ttccacaccc gtcttttgtt tcacgaacaa aagagcctct
    38281 ttcgctgctt gatattcttt cggataaaat ttttttgttt caaataataa tggattaggc
    38341 agttggatat tttcttttgc tcgtgtgatc gcataatgaa gatgatcggt caatgtcaga
    38401 taaatattag gcgaaaattc aacttttagt ttatcttcag ccagttcgac tacttgtttg
    38461 acccattcga tctcctctgc cggaatatca gccatcagct gctggaattg gtttttagca
    38521 aaagaatctc ttaaaacaaa ttctttttgg accgcttctg gcgaaatctc gtccccaatc
    38581 tttttttgaa aagctagtcc ttttcccata caaacgactt cttccccgaa tccatttttc
    38641 gaaatgacga cattgttatt caaaatttta tcaattttca ttttgcttcg ccctctcaag
    38701 aaaaataaaa aaacctagaa ttcgcctact gtttcttagc tgaattctgg ttttgcctgc
    38761 cgtacagtaa caatccattt cttattcaaa atcatcatac catcgaattt ttatttatgc
    38821 aagccttttc atataaaaaa taaaaaaact ggaacacaag tctatctaga aaacaaccaa
    38881 atcagagatg cacctctttg cgtttctctt tttttcagca aattggtttt tccttatcga
    38941 ttcatatcct ttggctgttt ttaatctgta acgacttatg tcccagtatc ctaaaatgtt
    39001 tttgtctcta gattgaaagc agatctcata tcatctgtcg catcttttat cgatgtaaca
    39061 ctttccagtt ctccttgtac aactagtcgc gtgacggcgt ccatgtcgcc acaagtaatt
    39121 agtgtgacaa tttcttttcc ctcgacttca ttcagtaatt gggtatctgt aggagcaact
    39181 ttttctttaa aaaatgtttt gtatgtatac acattttcta aatctgtcaa atagatcttg
    39241 gcacccatct ctaaattttc tagcggtgta aacagcaatt cagggttcgt tgcacgatgg
    39301 cttgctaagg cataatttcc ttttcccatt tcttgatctg gagataatgt tcctgctccg
    39361 taaagtagtg acgtattatc caagcctttg aaaaccggta aattgattga aactgaaggg
    39421 actgcgatac tagcaatcac tggtagatct gttcctctca tttgcgacct catgacccct
    39481 tctgtcgtca ttggttctac cgcatcaaag tcaaaactga catctttatc gttattcttt
    39541 ttcagatctt cttttgttac attcgctata gcatatttat ctcctgtctc tctgacaaaa
    39601 taatccttga tctgttcatt gaaaatcaaa gctagtccta ctaataacaa gaggaacaag
    39661 aggatattga ctagccatct ttttgctttt cctttttttt tcattttctc tccctccaga
    39721 ctcattttac tgtttttctt ttgctctttc caacagccga atgacacatt tctacaactc
    39781 tgtgtatttt tttcgtacac ctgcgctttt ttctacagga tatttcaaat tatttttgat
    39841 caatttttct tcaatgatct catctagatc caggtgaagg ttgtctgcca tcatgtatga
    39901 atagatcaat acatctgcca gttcttcttt gattcgttct aattgtgttt cttttacttc
    39961 ttccggcgtt ttccattgaa aaagttctaa caattcagat gcttccaaag aaatagagat
    40021 agccaaatct ttttcgttat gaaattgtcg ccaattacgt tcatctctaa actggttgac
    40081 tttttccata ctattcattt gcttgccttc ttccaatcac ttcttacttg attaatcata
    40141 ccattttttt acataaaaaa tagatgatgc ttttaaaaaa actcctgttt tcttttattt
    40201 aaaataaatg tcatagaaaa tcaataattt aggtagccac catgtgtatg cagtagcgtg
    40261 tcttcttccc ttgaggcttt ttgtttttcg atacgttctt cttcccaaac agacaacgct
    40321 actagaatct ctcttaatga ttttccttct tcggtgagcg agtactctac tttaggcggg
    40381 acttggttat aggcttttcg atggatactt tcatcttgtt ctaactctct taactgctgg
    40441 gtcagcattt tttttgtgat cgtagggatt tgcttgcttc tccacgccta aaataagcga
    40501 tattgctctt ccctttatct ggtgtaagca aagcagttag cattttgacg atcgtgggtt
    40561 ccgcttttca cttcttatct acttagtatc atgaagtata ctttcgtact aatactaagt
    40621 agcggaagtg tcaactactt tgtttaacta catagcaaaa gaaacagaag ggtgacaacg
    40681 gtcatcacgc ttctgtttct tttgaatatg ctgtgttgtt gtctctatct ccgtgtagac
    40741 tttcctcaaa cttatccttt gacagtaagt cagtaggctg atttgctctt tctagatgcc
    40801 agatacattt ctacaaaaaa atagtgattc tgttttgaaa attcgatttt tagttctgca
    40861 ttttcgtaaa catccaacat ttttttcgca ctgtatagac ctaacccgcc atttttttta
    40921 gaggtttttc ctcttttgac catctccgat agcgtcaaat tcgttggatt tgccttgttc
    40981 gctatactaa aacgtgatcc ctctttttct tccagcagat tgatcgtaat cggactctga
    41041 tcggctacac gatgatcgaa tgcgttagaa ataaaaatag ataaacagcg aataaaaaca
    41101 accagatcaa tcgggattgt tttcacttcg acaggaataa ctacttggat cgggattttc
    41161 tccgattctg ctttttctag aaattttaaa aagacacccc tgacagctgg ggatacaata
    41221 tttgttagct gttgtatttg cggtttttca aaaatagctg cagaatgatc ttctaatatt
    41281 tctaaaaatt ccaacgtttc ttttttattt tcctgaagag taatttttaa tgaaaacagt
    41341 agacttttat aatcatgttt gaattcctct agatgatggt aatattcttc atttatttga
    41401 tacagttgaa gcgattcctg atgttgtctt tcttttactt ggaaactgat caacataatg
    41461 gaaaaaaaca aagaaaaaaa actgatgact atcgacaaaa gagatagaag cagataatta
    41521 gatgagcgga agtgaacact tcccaatacc aagtaaatca cgccccagct taataaaata
    41581 caaatacctc caaaatagtg ttcagttgga taagtaaaaa gcaagttgag aaatctcgtc
    41641 tgtttgtcta tactttttac taatagatag aaaataaatg cgataagtag tgcaaaaaaa
    41701 cttgctgttc caagatgtct ctttcctaaa taatccatca gatcaaaaac cagaaaaaag
    41761 ctgagtccag tgatagcaaa aaaaagatta ctgatcaaac agctgataaa caggtttttc
    41821 gtagtaaaat aaaaaagaaa catcaataat aagccaacta ggtaaggaaa gctcacttta
    41881 gggataggaa gtaaaagtgt caccaccatc aaagctatac aaccaaagct gttttttact
    41941 agcagttgtt tcgaaaagaa taaatagata cctaaatcta aaatcaaagt aaaggtcaaa
    42001 taaagtgtca ctgttgcgac catcttattc ctccattgtt ttgtttcaaa atgtacacat
    42061 atttttttaa taggagcctg catatgaaaa agtattttat catagaagat aaccagtttc
    42121 acagcgaatg gatcatcaga gaattggtca atcatcatat cgagaatatc gtgcagctca
    42181 cttctatctc tcaaatcaat cagttgatcc agttgggcac tattgataat cacgatgttt
    42241 tctttatcga tatcgacttg aagcaatcac tcacaggtat tgatgtcgcc gaacaaatac
    42301 gtaaaaaaaa tgcacatagt aatattgttt ttttcaccgc ttatacgaat tttgcagtag
    42361 agatcatcaa tcggaaaatc cttccgattg gatatatcag aaaagacagg aatttatctg
    42421 aacaaattcg tcaagtgcta gaacagatca attggaaaga acagcagatc gtaaatgtcg
    42481 ataaaatctt tattgatcaa aaaggtggaa gtctcgtcct gtatcctcgg gaaattttat
    42541 atattgaaag tatcaaaggt ttgaaaaacc aagttatggt caaaacaact acagaagata
    42601 aattagtgaa tacttcttta aaacaattga agacagagtt agaaaagtat gcttactttt
    42661 tacttttaaa atcttactgt atcaacttat cgcaattgca gaaaattgat tctgctcatt
    42721 atgtgttaga atttttcaac ggtgactcct tactcgttgg tcgaaagatc tttgaaaaga
    42781 caaaggaacg tttccatgac ttccaaaaaa ctgcttcgag ttgatgaata agagctgaga
    42841 cgtaagcata gcagatttca gggagtgggc cttgaatcgg ctccactccc tgaaatcgtt
    42901 gatatgattt tacgcttcct cttctgtagg agcatctgtt aacgaccatg tgaatgttga
    42961 aacgtagttg tctacagcat aattggatac aggaacaaat aattccactg attgtctccc
    43021 ttgttccaca ttctctccta atgaataaat ccatgtgcca atcccttcat ttgctcctgc
    43081 agttactaga tctactgcct gatctgctgg gatttcgatt ccttcagcgc cagttccaga
    43141 aacggtagat ggcattccac tgctattagt tgcgctattt cgaatcacgg cgtttttgaa
    43201 tttcaccttt gaaccagtta gtttattcgt atcagatgtt gtaccaataa attctgttcg
    43261 tttgactgat aatttccaac cggcataatt tccgctttta tccgttactt gtacgaaatt
    43321 ctgtacttgt gtttgtgctc cagtactgtc cgttccgctt tgtaatgcag catatactgt
    43381 ttttgctgta cctaacttga ttgttccaaa atcaagatct ggagcttggt caatcgtcag
    43441 taatccggat gttccctgat ttggttcgtc aggtgtaaaa ggagtatcag gatcatttgg
    43501 atcaacagga ggaactggaa caacgtcttc aggatttgca gcatctggtt tggtcacaga
    43561 aatcgttcct tctgtagtgg ctgagctctt attctcagca aaaacacttt gtgtacataa
    43621 taatagactt ccagctagaa cggtacttaa tgcaattgtt tttttcattt ctttttcctt
    43681 ctttctttta aaataaataa tccagcaaat aagccaataa gaatgactcc aacaagtatc
    43741 agccatcgcc agtcaaatgt ttctggctga ttttttaaat caatatcctt ttcattcaat
    43801 tggtccgctg catcatcagt tatttcaaaa tcggtatgga gattccagcg tttttctttc
    43861 caagcaccct tcacaaccgc ttgataatgt cccggagcta atttttttcc ttctaatggg
    43921 acaagtaaat caaaatttgt attgggtgct acatctattt tttctttttt tattgaaagg
    43981 taaggctctt ttgtttcttt tttgtagatt tcaaccgatg tagtcaagcc gaacatataa
    44041 gaagcatttg gattttgaag attcgcctta atcacatttc ttccatcgct ttgatccgca
    44101 taagcttttt ttaatttcaa atctggggaa gactcttttt cgctatttca aatcaagatt
    44161 gctttcactt gagaaaactg attgacgaca ccaactgtac ttttgttaga aacgttctct
    44221 tttttttctt gaaacgtgat ccctcctgcg accacaccag ccagctcttt ttctggcgct
    44281 ttcagtgaaa gtgtctcagt gaccgcactt tgaggaggaa cagttatttc tcccactgtt
    44341 tttgctaagt ttttcatatc ataacttaat gtttcatcaa atttactttt tgattctcca
    44401 tattcgatca caccgtttga attcgtcgta gcggagtgga tcaatgtctc aactgttact
    44461 gatttatctg ttcggttaaa gaccgtgtag gggatttcct gtgttttccc aggctccatc
    44521 cttacattta tatatgtttt gttcttatca atctgattag ccggtaattc aaaatctacc
    44581 gcaaaattga attcggaggc atggacaggt gtctgccaaa ggatgaatcc aaggagaagg
    44641 atcataagta aagagcagtt gtttcgatga cttttattcc atattcgatt catcaatcat
    44701 cctccttgat ctttattctg tcggcgcatc tgctaatgtc catgttatct ctgctgagta
    44761 attaccagct atctgatttt ctttggttaa gacaagttta aacgcccgac cgttatttaa
    44821 atattctgaa taatctgctt gcacattcgt tgaattttgt cctctgacaa tttccagttc
    44881 ttcttcattc actaattgat actcctctcc tttcttgaaa tataggacat cctgcataga
    44941 aaatcctgca tcatttgtga aaggctgaga taattttatc gttaagatcc acggattttt
    45001 cgtttctcgc tgatcataaa tccataattt ttcctgtata tccatttggc caaatgcttc
    45061 ttgtgtcaaa ccagttgctt tgatctgacc aaaatcgagt ttggaagggg caagaaatga
    45121 gagaccacct tctactacga tcttcgtagc aggtgcaaaa ggcggggcat catgataaac
    45181 aactggcgta tcacttggat tgtgattccc aatttcatcg ttggtacttg gcttgttctg
    45241 cacaccctta gcagtagctt ctcctgcttg gtcgtttaat acaatttcta atacatctcc
    45301 tgcaaaaagt tgattttctg ggatagaaag aacaaattcc cctgtatcat caatagttgt
    45361 ttctgacaaa acagcttgat tttttttcaa tgaaaccaag gctcctgctt cacctgtacc
    45421 tgtgatcgtg cgagaagctg cattgatcaa tgtggtactt aaattagcag gttcaggagg
    45481 aatcacatcg actgtcgttt tggcagagtc cagttctgtc acttcttggc cacctcttac
    45541 ttttttagca gcaacaactt gcacttgatc ctccgttttt aatctctcat cattgggtac
    45601 ttgaacttcg aacaagccgc cttgttcgcc ttctccccat gggttatatc ctgccccttc
    45661 attttcatct gtttgtcctt gcgtgagagc agtaatttct acagatgatt tcgtcgtatc
    45721 tgcgtaagta atacgtaaag tcacttctac ttcgcctgtc caagcatctc ttggctttgc
    45781 ctcgacacct tcgggaacaa cgacatgccc atagattttt ttatctgcat tcgttggcac
    45841 tcttaaggat tcaatcatcg ggagctgatt attggcactg attcgattat aatttttcat
    45901 tccggcacct gtctgagcaa attcatttgt taaacctgca ctagggcttg atacgaaatt
    45961 gcctaagtcg gtcccattga tcgataagtc tgtcatatca gaaaaataat ccgggtcttt
    46021 ttcaaagtta tcgaatgttg cgcctgtttt tcgccataaa gcgatttctg aatgggcgat
    46081 ctttaaagaa gaatctccat ttccttggaa tacccagccg cctatgccat tggtctcgct
    46141 tccaatccgt ttgttttgga aatcaaaata ggtaggagat tctaagtcca cattcaacac
    46201 atccccactg atgatgccgg tcttatcttc ccctgttttc ccgaccatga ctaaataagt
    46261 tttttcacct gccgtaaaat ttaaatcaat atctccagtt gtatctacag ttgctccgga
    46321 atctgcgcgt atatcgatat ttgatttttc tccagtgact gtaaaatcag ctgaacctgg
    46381 actttctaga aaaccagaag cgtcataaaa ttggatcgct tgatttcctt gattgagtct
    46441 gttcacgtaa gatttgccat cacctgtatt tcttaccctc aatgagcctc cattggttac
    46501 tgtcagcttt tgcgtaccat tctcaaaacg aactgcgctg gaacgcccac tctgcttatc
    46561 gatgtcgata actgatccgg ctccttctac gatcaaagaa aggccttttc ctacaaaacg
    46621 catcccattg ttcccgggtt gatcgtcatc tccagccatt ttgaccgaga tttgtccacc
    46681 acttgataca tggatttgac tagaatctcc ttgcaagcga aatccgcccg tagttgctgt
    46741 attgatggtg attttcccac cattcgatac agtgactttc gatcctgaac ctctcatttg
    46801 gatgattccg cgattgttgt cattggtaat attgatcttt gtatttaccg aaaattcgct
    46861 tcccgaacca ctgacttcaa acatgacatt accaggtagc gtatcgactg cctctccttc
    46921 atcgctaacg ccatctgccc gatcataatc aagagagacc tttgaaccgc ttaagatatg
    46981 gattcctggc gctgtttttc cactaccagt ctcatctcct gaatctagaa aaccgtagaa
    47041 tttccctaga tacggtccat aaagttgact accattgatc acattcaatt gatagcttct
    47101 acaaaaatag gctttacccg gagcggcatt cagtccttcc ttcactggtt ggacatccaa
    47161 cacaccggaa acaccatcta atgtgacagt tgcacgagga gcgtaaatca gtcctaattt
    47221 ggacccatca tacaaatcaa gtgtcccggt aaattttatt tctcccgtaa aatgatatct
    47281 gcttccccaa aaatcggtca tataagcaat gataatggca tttccatcga tatttggtgt
    47341 caatccacct ggatgtgcag ctgttccttt aaaattctgg aactcgatcg actgaggtat
    47401 ctcattgata taaatatggt gtgtgccgaa attgtgagcg aatccaccaa aatcaataac
    47461 gatatctcgt ttgatctcta attccctgtc taaagtcaaa ggctgaatca gtcggatctt
    47521 agttactgat tcgtctgcga gaagctgcgc cagttcttct gttgtggcag cttcgctgta
    47581 cccatccgct gaaacagctc ttgccagtgc cgcttcactg ctatcagacg tttgttcagc
    47641 tacttctgag gtctcacttg tcttattttc ttcttgcctt ttattagtag aagcctcact
    47701 ttgagattct gtagtttcag aagtaatcga ttcttctgaa aaagtagtct tgtcaaaact
    47761 ttgattctct gattgcgttg attcgtttgt tttctctaca tcggcaaaaa cggatgtcac
    47821 catcaagaga gggaagacaa acaaaatcga cgcaatcagc ttttttacac attgattcat
    47881 tctccaccat cctctctgac gttttgacaa ttttatttta tcgttttaat ctatttccaa
    47941 ttgtactttc atatttaaaa tatgtatttt tatggaaaaa tttttctatt aggtgtaaat
    48001 ttcttttttg tacaaaaaaa aggctgtgac aaaagtcttg tcacagtccc ttatcccgaa
    48061 caagcggtgt tctcccgcca actcctcggt cacaacctcc attttaacaa taaacctgta
    48121 aatgtgaggg caatactttg attgtcacag gtaatttatc cccttcatct ccatcaacat
    48181 tcgtcgtcaa ttcttcctca tcagctaatg acacaactaa ttgctcacaa gtaaggtagg
    48241 ctagattttt cgtagattca tctactcctt ttaataggtc tggcaatgtt ttaagcgtat
    48301 cgatcaaaga agaatctttt aaataaacca aatggagctt accatcatcc acttttgcat
    48361 ttggaagtaa tgtttcaaaa ccgccgactg agttagttag tcctatcagc aaggtactgc
    48421 tctcaatttc ttccttcttt ccatcaactt ctacgtgaaa cgagtaggat tgtgtggaag
    48481 ctaactgttt gattcctgac atgaaataag ctagttttcc aaatttcgtc ttcttctccg
    48541 catctacatc attgattgat tcagggatag agccaattgc gacgacattc ataaaatagt
    48601 cgtcatttat ttttccaata tccaatggct tgactgattc aatcgaaaaa tgattgatcg
    48661 cttcctcagg ttctagcggt atccctaatg ctcgagccaa gtcattcacc gtacccaatg
    48721 ggaaaaatcc gaaatttggc cgatgttctt gttcagcgat tccactgatt ccttcgttga
    48781 ctgttccatc tccgcccatg acaaacacac tatgataccc ttccgtagcc gcttcgcgag
    48841 taaagttttt tgcatcccct gctttttttg tatgcaagac gaccacttca tcaaacaccg
    48901 attccaattt tgctatcgct aattgctcaa attcctttgc ttgttcaccg cctgaactgg
    48961 ggtttaccac taataaaact tttttcatca ctttctttcc cttctgattg atttctttaa
    49021 tataacgata gcataaagaa aaaaattagg ctgtgatgaa gtactgttca tgaaacattc
    49081 atttattttt tgacaagata aaacatccat actatgaaac tgaggaaaat taaaacaatg
    49141 atataggaaa taaaagaaaa tgataggcat tgtataccta ataaagtaaa aatcttaggt
    49201 ggcagataaa aacacaaaaa tagcaagaga atgtaatagc gtttcttcca ctttttgtta
    49261 tcagacctta tagcgatcag aggaatacca aatgcataac tgaaaaactc ttgatgtact
    49321 gttaatatgt cacggccttg aataataaat gcgacaatcc ccgctataat tagtgtgcct
    49381 aacaccaaaa cggaaaattg atgaatatat tttatataca aatgatccat tgtacttcct
    49441 gcctttttat ttgtgagaaa aacaaatccg tatacgtgaa taacttctag aaataaaaga
    49501 aatttgcaaa accactccat ttatctactt ctacgtgcat tttttgatat tgttttct
//
LOCUS       DAZULO010000011        48716 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000011 DAZULO010000000
VERSION     DAZULO010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.011"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(130..1896)
                     /locus_tag="ACG8OA_000655"
     CDS             complement(130..1896)
                     /locus_tag="ACG8OA_000655"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4975495.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1856..3609)
                     /locus_tag="ACG8OA_000656"
                     /pseudo
     CDS             complement(1856..3609)
                     /locus_tag="ACG8OA_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(3719..4288)
                     /locus_tag="ACG8OA_000657"
     CDS             complement(3719..4288)
                     /locus_tag="ACG8OA_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFS4975496.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4306..5721)
                     /locus_tag="ACG8OA_000658"
     CDS             complement(4306..5721)
                     /locus_tag="ACG8OA_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4975497.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(5732..6400)
                     /locus_tag="ACG8OA_000659"
     CDS             complement(5732..6400)
                     /locus_tag="ACG8OA_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFS4975498.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6530..7114)
                     /locus_tag="ACG8OA_000660"
     CDS             complement(6530..7114)
                     /locus_tag="ACG8OA_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4975499.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(7445..7678)
                     /locus_tag="ACG8OA_000661"
     CDS             complement(7445..7678)
                     /locus_tag="ACG8OA_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS4975500.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(7693..8643)
                     /locus_tag="ACG8OA_000662"
     CDS             complement(7693..8643)
                     /locus_tag="ACG8OA_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFS4975501.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(8640..9482)
                     /locus_tag="ACG8OA_000663"
     CDS             complement(8640..9482)
                     /locus_tag="ACG8OA_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFS4975502.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(9484..10203)
                     /locus_tag="ACG8OA_000664"
     CDS             complement(9484..10203)
                     /locus_tag="ACG8OA_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS4975503.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            10751..11053
                     /locus_tag="ACG8OA_000665"
     CDS             10751..11053
                     /locus_tag="ACG8OA_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4975504.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            11125..12351
                     /locus_tag="ACG8OA_000666"
     CDS             11125..12351
                     /locus_tag="ACG8OA_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4975505.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(12439..12831)
                     /gene="rpsI"
                     /locus_tag="ACG8OA_000667"
     CDS             complement(12439..12831)
                     /gene="rpsI"
                     /locus_tag="ACG8OA_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS4975506.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(12847..13275)
                     /gene="rplM"
                     /locus_tag="ACG8OA_000668"
     CDS             complement(12847..13275)
                     /gene="rplM"
                     /locus_tag="ACG8OA_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFS4975507.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(13809..14276)
                     /locus_tag="ACG8OA_000669"
     CDS             complement(13809..14276)
                     /locus_tag="ACG8OA_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFS4975508.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(14396..15934)
                     /locus_tag="ACG8OA_000670"
     CDS             complement(14396..15934)
                     /locus_tag="ACG8OA_000670"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFS4975509.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16014..16700
                     /locus_tag="ACG8OA_000671"
     CDS             16014..16700
                     /locus_tag="ACG8OA_000671"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFS4975510.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(16901..17428)
                     /gene="msrA"
                     /locus_tag="ACG8OA_000672"
     CDS             complement(16901..17428)
                     /gene="msrA"
                     /locus_tag="ACG8OA_000672"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4975511.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(17512..18189)
                     /gene="pgmB"
                     /locus_tag="ACG8OA_000673"
     CDS             complement(17512..18189)
                     /gene="pgmB"
                     /locus_tag="ACG8OA_000673"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS4975512.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(18186..19316)
                     /locus_tag="ACG8OA_000674"
     CDS             complement(18186..19316)
                     /locus_tag="ACG8OA_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS4975513.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(19316..21499)
                     /locus_tag="ACG8OA_000675"
     CDS             complement(19316..21499)
                     /locus_tag="ACG8OA_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFS4975514.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(21496..22521)
                     /locus_tag="ACG8OA_000676"
     CDS             complement(21496..22521)
                     /locus_tag="ACG8OA_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4975515.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(22566..23393)
                     /locus_tag="ACG8OA_000677"
     CDS             complement(22566..23393)
                     /locus_tag="ACG8OA_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS4975516.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(23390..24457)
                     /locus_tag="ACG8OA_000678"
     CDS             complement(23390..24457)
                     /locus_tag="ACG8OA_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFS4975517.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(24483..25319)
                     /locus_tag="ACG8OA_000679"
     CDS             complement(24483..25319)
                     /locus_tag="ACG8OA_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4975518.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(25316..26185)
                     /locus_tag="ACG8OA_000680"
     CDS             complement(25316..26185)
                     /locus_tag="ACG8OA_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4975519.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(26255..27538)
                     /locus_tag="ACG8OA_000681"
     CDS             complement(26255..27538)
                     /locus_tag="ACG8OA_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4975520.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(27552..29237)
                     /locus_tag="ACG8OA_000682"
     CDS             complement(27552..29237)
                     /locus_tag="ACG8OA_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFS4975521.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            29512..30537
                     /locus_tag="ACG8OA_000683"
     CDS             29512..30537
                     /locus_tag="ACG8OA_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4975522.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(31132..34785)
                     /gene="rpoC"
                     /locus_tag="ACG8OA_000684"
     CDS             complement(31132..34785)
                     /gene="rpoC"
                     /locus_tag="ACG8OA_000684"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFS4975523.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(34864..38481)
                     /gene="rpoB"
                     /locus_tag="ACG8OA_000685"
     CDS             complement(34864..38481)
                     /gene="rpoB"
                     /locus_tag="ACG8OA_000685"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS4975524.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            39209..40177
                     /locus_tag="ACG8OA_000686"
     CDS             39209..40177
                     /locus_tag="ACG8OA_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4975525.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            40195..41100
                     /locus_tag="ACG8OA_000687"
     CDS             40195..41100
                     /locus_tag="ACG8OA_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4975526.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            41097..41903
                     /locus_tag="ACG8OA_000688"
     CDS             41097..41903
                     /locus_tag="ACG8OA_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4975527.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            42060..43028
                     /locus_tag="ACG8OA_000689"
     CDS             42060..43028
                     /locus_tag="ACG8OA_000689"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFS4975528.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(43345..43869)
                     /locus_tag="ACG8OA_000690"
     CDS             complement(43345..43869)
                     /locus_tag="ACG8OA_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFS4975529.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            44156..45967
                     /gene="scm"
                     /locus_tag="ACG8OA_000691"
     CDS             44156..45967
                     /gene="scm"
                     /locus_tag="ACG8OA_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFS4975530.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(46122..47108)
                     /locus_tag="ACG8OA_000692"
     CDS             complement(46122..47108)
                     /locus_tag="ACG8OA_000692"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS4975531.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(47131..47697)
                     /locus_tag="ACG8OA_000693"
     CDS             complement(47131..47697)
                     /locus_tag="ACG8OA_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFS4975532.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(47711..48127)
                     /locus_tag="ACG8OA_000694"
     CDS             complement(47711..48127)
                     /locus_tag="ACG8OA_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFS4975533.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
BASE COUNT        14556 a        10021 c         8505 g        15634 t
ORIGIN      
        1 gtgaactaat tcaatcatac tgattctttc gtgtattcaa aattagatgc tgtttaataa
       61 aattattctt tttgaaaaag aattataact atatcccaaa aattgtaggt taatttaaag
      121 cctatttcat catcgtgatt gagcttcttt ttcatagacg aatttctcag atagtttagt
      181 tttctttatg agatttttgt atatttctga ccttcgatac aactcattat gagtaccttg
      241 atcaactagt tttccttgat ccatcactac aattttattt accttttcaa tagacttcaa
      301 gcgatgcgat ataattagta ctgtcttatc ttgtattaaa cgatttaaac tttcctgaat
      361 acgtttttca ttttccgcat ctaaactggc agtaatttca tctaaaatta aaattggcgc
      421 atttttcaaa aaagcccgtg ctatggatag gcgttgtcgc tctcctccag aaagtgaact
      481 tccgttttct ccgaccaatg tatgataacc gtcaggtagt ttttcaataa agtcttcgca
      541 gttagctaaa cgagctgctt ttttcacttc ctcatctgac gcttgactat ttccaatacg
      601 tatattttcc aaaatacttg tattaaacaa agttacctct tgaaaaacaa tagcgatttt
      661 gctaaacaaa gaagctggag atacacgttt aatatcatac ccatcaatta aaatacagcc
      721 ttcatcataa tcatacagtc tagacactaa tttcaagata ctagttttcc cggaaccact
      781 agcacctact aacgcagtca cttctccttg tttagccgta aaagagatat gatctaaaat
      841 tggggtattg ttatcatatg agaaagagac atctcgcaac tcaatgtcaa aagatttcag
      901 aggactatct gatccctctt ggatactggt ttccttcata gctctaattc gctgtatttt
      961 aggagctaaa taaaatattt caagcactgc ttctttcata gaatcgaagg aatcctttat
     1021 ttttattgcg gctagtaagt aaccaacgac gtagagtata gtcacttcac ccgttaacag
     1081 taagtgaaca cctactagaa tgactactgc taaactaaca tatgagaaaa tagaggataa
     1141 ggccatgact gaaaaagtac ttaattctac ctttaaatga atcctttcac tctcttccat
     1201 ttttttgaaa agtctgtctt gaactttttt agatagatta aagctattaa tctcttgctg
     1261 caattctata gtttcttgaa attcttccga gttttctctc aaagtatcgt aatatttagt
     1321 attggctttc gtttgggatt tctttgataa caagattaag acaaaactaa ataacgttgg
     1381 caaaataaca gctaatccca ttttgacatt accaaccaaa agcatcactg aaataaaagg
     1441 gaaaaacaga gccataccgc cggctttagg tattgcatga ctcatcgcat gctcaatacc
     1501 ttcaacgtca gacatgatag tttgagataa atctgataaa ttatgttttg aaaaatagga
     1561 taatggtaaa tttgacaaat cgtctgcaat ttgcactctt aaatgcgcac tttctttata
     1621 agtactatta tataagtttt catactccct atctaacaac caatacatga aaagcaaagt
     1681 aacaaacgat acggcaaaat aaagccaacg gggctttgta ttttctaaaa ctaattcgtc
     1741 tatcagcatc attataatga atgctggtac catattaatg cagtatacaa aaaaactaga
     1801 aatagaagct ttagttaaag cttctgcgcc ctgatcactt aaagcaaatt tgtttttata
     1861 aaaacttttc attgtcaagc ctccaatcat ttgcattctc ataattagtg accaattgtt
     1921 tatacacttg atgtttagcc attaattcat tatgatttcc acgttctact attttccctt
     1981 ctgacaagac aagaatttca tctacagatt taatgctgga taatcgatga gctatcataa
     2041 taactgtttt ttctttcatc agatgttgaa aagccttttg tagttcatac tcattatctg
     2101 gatcaataga tgcactggct tcatccataa taacaatctt tgcatctttt aaaattgctc
     2161 tggcaatagc tatacgttgt ttttctcctc ctgacagata aactccttta ctaccaatta
     2221 aggtatgctc tctttcggga aatttttcta aaatcgattc gcaaccggct aatcttagcg
     2281 cgtcaaatac tttacttgct ggcgcttgag gatttgctat agaaacgtta tcgtaaatac
     2341 tctgattgaa tagtttcgta tcttgaaaga caaaagcaat tgtgttaata attgtgtctt
     2401 ttgaatagtg atttaaaggc ttaccaccaa ttttaatctg tcccttatcc acattgtaga
     2461 aacctgaaat tagtttagct atcgtactct ttccgcttcc tgattttcct actaatgcat
     2521 aaattctttt ttgttctaaa actaagttga aattttctaa aacacactct tctttgtatg
     2581 aaaagctgac attttcaaat gcaatgtcac aatttttaat ctgtgtctcg ttcccatatt
     2641 ttagttggtc ttgcttcatt tctaaataaa gggagtctag tgtttcgact gcatatcttg
     2701 cttcaaacaa gtgcatactt acatacatta ttctcatgaa agaaacaaaa ataattccac
     2761 ttaaaaaaaa caacatcaac aagtcaacag ctaatatctt gggagtggta agagaagtta
     2821 taaaaaaggt tataggaact atcaaaatgc aaactatgcc aaaaaatagc cattgaaaaa
     2881 gaacaaaagg tgttttccca ctcaaagaat acttatatgc aaacttagca taatcgttaa
     2941 ttgctttatt caaggcttta aatgatgtta ctttagcacc aaatatcttt ataacttgaa
     3001 ttccgcggat atattctacc gtttcagagc ttaacacatc taacgattct tgatattttt
     3061 gaataaaatt tgtattctct gtcattttct ttaacaagaa tacaccaatt actaacatgg
     3121 cgattagaac caatccaatt ttaaagttaa tgaaaaagcc taaggcaact gataaaacag
     3181 gtgttactat ggcttgagca ctatctggaa tcaagtgagc aattgccata tgtgttttag
     3241 ccgcgttgtc atcaattgtc tttcgaatat atcccgaaga atttaaatca aaaaaacgaa
     3301 aacttgcttc tgttaaaccg ctaattccct tttttctcaa attagtctct aagcgaaacc
     3361 caaggatatg agaaaataag ccagaaaaaa tataaaataa agctctaaag ttaaccaagc
     3421 aacagtttga accgcataac tcttagctaa atattcatct cctacaataa ttagagactg
     3481 aagaaatttg taaatactgt aatatccata gactgttaat acgacagaaa aatcagaaaa
     3541 taaagtggct aaccatccgt agtatttcat tttagggaca taactaaata attttttgta
     3601 tactttcatt ttttcctccc ccgcattcaa tttcttctct tattcgttta caactgttaa
     3661 tgctttccgt aatagcgtaa attagagatg tacaaacaga taaaaggtaa actacgttct
     3721 attttttgta tttttttaag acttttcttc caatcaatac accaatcata gataaacttg
     3781 ctacaactaa cactgaaatg attccccata tacctaccgc atagtggatc attgcatcca
     3841 ttgttgcagc agattgtcct ttatctatcc aatcttgacg aaaacgatct gtaaagaacc
     3901 atataggaat ccactgtccg gcagcactgc ctaattgcat tagagcccaa gacgcaactt
     3961 gaggaattgc tcgatcatat ccaaatacag atagaataat atcagctaag atcgccatac
     4021 taatcgaact gataaaccaa ggtaagtacg ccccgcccat taacgtaaaa aataacaata
     4081 aaacaattgt gtaaatagta aaaattcctt ttctagggta gttatagcta atataaacaa
     4141 atatgattcc agataataat ccaaaaatcc cgggggatat gctgtgacca aacgccccac
     4201 caatactact aaccattatt gaaaaaaagt aaattactag ggaaataact gtagctaaac
     4261 caataaaaat ataatctttt gttttcatat atattctcct caattctatt tcgttatgtc
     4321 attaaaaatc tgttctatca aagttgaatc ttctactcta agttttatat ctttttttat
     4381 tccaccattt tttaaataaa taattctatt gcatgtttta taaagaaatt ccggatcatg
     4441 agtagctata actactgcac ccttttcttt taagtcttta ataaggtcag caatcctttc
     4501 catattaata taatccaacc cacttgtagg ttcatcaaat agaaataaat tagtttttga
     4561 caatgaagct atagaaacca ataatctttg tttttctcca ccggataaat caaacggtaa
     4621 ttgatctttc attttataaa gttccatttt ttttaaaatt ttttcttgct gatctttact
     4681 aactatttca ttccccaggg ctaactcgct cctcactgat tctgtgaaaa attggtaatc
     4741 catttcttgc attactaaaa acggcgatga caaatttttt ttattttcga ttatctttcc
     4801 tttggtaggg cgaataatct tcatgagtat tcttagtaag gttgttttcc ccacaccgtt
     4861 tttccctacc aaaccaataa catctccaac tttgacatca aaatttatgt cttttagaat
     4921 attttcataa gtaatttctt caacttttaa tacatctgca cttacctttt cgatataggg
     4981 aacttcaatc gaactattaa aaatgtcaaa tttccttaat gcgttattgt tttcagtttt
     5041 taacaactga atagttgtag tttttacagt tctattttta acaaatagaa cttgatcaat
     5101 taattctttc aaatagtaaa aacgatgttc agctataaca atagtaaatc ctttattttt
     5161 taaatcacaa agtaagtcag caagtttctt agtcgctttg aagtccaaat ttgaagacgg
     5221 ttcatccaat ataattaatt tagagtttag cgataaacta catccaatag aaattttctg
     5281 ccgttcacca cttgatagcg aaaaaatgtt tttatctaat atatttgaaa gatctaataa
     5341 ttcagctaat tcattgattc ttttatccat atgctctttt gtaaatccaa agttttccat
     5401 cgaaaatact aactcactcg ttgtatttgt tgtaaagaat tgtcctctag gatcttgact
     5461 tacgtagcca atattttttg atatactttc ttgtgttaag gattgtagcg agttattttc
     5521 aaataaaatt tccccaacca attttccgtc ataaagtctt ggaataagtc cattcattaa
     5581 ctttaatagt gtggatttcc cactcccaga agaaccagta atcaatgtaa gactgccttt
     5641 ttttattgaa aaattaaggt caacaagcgc ttgttcctta acatccttat atgaaaaaga
     5701 aacattgtta aattcaaaca attctttcac ctcaaatcaa aatacttaag aaaaaaatag
     5761 taacggaaaa caggatgata tagtctaata agctcatgtt tgttttatag taagaacttc
     5821 ttttcacagg agattctatc ccctttgtta ttgcattagc ggtcatttct tcagcaatca
     5881 tagttgcacg tataagaacg ggaactaaca gaaattccaa atattgcaga ggctgaaaaa
     5941 aagacaagcg aatgtttctc atttttaaag attctttaat cattcttatt tcagaacgat
     6001 aagtcggaaa gaatctgatc actactgtaa tacttaacat tatttttttc ggtaaatgta
     6061 ttttttctaa agaggataat aattcattgc tagtatagac actaaccaac aaccatgaga
     6121 tcattacgac cggcaacatt cgaaatacaa tatataagga tgtcgcaaaa aatatagtaa
     6181 aaaactctat cttgatagca tcaataattt tcattactaa aaacagaact gaataaatca
     6241 agatgaaata aatacatgtc tttattttac gaaacatcaa tactaaaact gtagcaataa
     6301 tgaaacaccc cacttgaaat atatttccct ttcctaaaaa aataatgacc ggaaaaaaaa
     6361 tatttaaagt aatcaaggtt aacggattga tttttgacac tctttcacct ccaaaaaata
     6421 agatcgcact gttatcttaa gctgataaaa atgcaccgtt attcagataa cagtgcgatc
     6481 ttatcaaaaa gcataactga gaacaattct caatgcaagc tttttttaat taactcagta
     6541 attcaatcca cccaggaaca gaaaattgat acaatcttct ggttaatagc atagtctttt
     6601 cttttgaata gctatgcttc aatatatcat agaatgtagc gtagaatgag taaatgtaga
     6661 agtggatttc ttcttcatta aagactaaat tttgattgat cattccgata gtctttaact
     6721 tatctagaca acagactgtc tttttcgtca caaatgatgt aattttttca acaatatctt
     6781 gatatttggt tccagctgat tggaataaaa tcaaatcgaa tatacgttta ttctcaaaaa
     6841 tataattaat aaatggatct aattcattga agttctctct ccaacttttc tctatatcta
     6901 tattttctaa gtttaagagg tatttggtaa actcttcatt atacatatta ataagcgcat
     6961 taatatgcgg ctgaataatt tcttcaaata acgcttcctt agaagtaaaa tgtttataaa
     7021 aagcacccgt agttacgcct gcggtcaggc acagttgcct tatcttagtt tgctgaaatc
     7081 cttctttttc aaaatggctt agtccacttt ctattaactt ttggtgagtt ataatataat
     7141 ctctggtttc tatgggtact tcctcctaat aagatgaatt aactttatta tcttacacag
     7201 gacttgaagt ttcaatcgtt aacgtttatt actgaactca ttccttagag ctaaactaat
     7261 gagaaagagc agaataactg atacaaaaag caatcctaca agaattaata gactcatcta
     7321 aatcattcac cttttctaaa ataatccgag aaagatttaa aaaaattata tccagctatt
     7381 aggataagtc ctatcataat cactccaata gccattttct tatcctccct aaaaataaag
     7441 tcttttactt aaaacctttt ttgtaatcgt caataaattt tttcccttta tcagttaatt
     7501 gaaatcctat atctaaatag tcctcatatc taagtttagc cagcatctca cttacagatg
     7561 ctggactaac acttagaact tttgctaaat cattattttt taatttcttt tttctttttg
     7621 ataattcctg aattgctagt aaataaatta gtatagtagt cgttttaatt tgtaccatac
     7681 ctaccctcca aattatttcg aaaggccttc agcaatttta tccaggttcc atttcatcat
     7741 cgcatagtaa ctatcaccat cttgtccttt ttctgcaatt gaatcagtaa aaatcgttga
     7801 atagatagga atattggtat cttttgatac tgttttcatt ggtctatcat ccacactgct
     7861 ttctacgaat aaggagggaa cttttgttgt gcgtaatttt tcaactaagt gttttatttg
     7921 atctggtgtt ccttcttctt cagtgttgat ttcccaaatg taagcagaag gcacattata
     7981 cgctttcgag aaatatttaa agcatccttc acttgtaaca atcatttttt tatcattcgg
     8041 aattgaagca aatttagatt tagcttcttt gtctagagaa tccaattttt caatatactt
     8101 atctagattt tctttataga attttttatt atcaggatct ttttccgcta actgtttttc
     8161 aatattttta gcgtaaataa taccgttttc taaatttaac caagcatgag gatcttcctt
     8221 ccctttctca ctctgaccct ctaagtaaat aacatctatg ccatcacttg ctgcaaaata
     8281 gtctttgttt tcctctttgt tcgcattttt aactaatttt gtaaaccaag catttcctcc
     8341 agtttccaag ttaacaccgt tataaaaaat caaatctgct tttgaagttt tttgaacatc
     8401 ttcaggcaaa ggttcatatt cgtggggatc tttcccaata ggtacgatac tgtgtaaatc
     8461 aattttatct tttgctatat tttcggtaat atctgctaaa atcgaattag taactactac
     8521 ttttaatttt tcgttcgaag ccactttatc tttgtcttta ctatttattg aacatgcccc
     8581 gaatataaaa agagcaatca aaatccccag acttcccagt actgctttcc attttctcat
     8641 cataacgatc tctccttctt tcttaaaaat ttttgttttg gtgagattag aaaactaatt
     8701 acaaataaga atgccgctgt taaaacaata cttgatcctg ctgctatgtt aaaactataa
     8761 ccaataaaca atccaagcaa tgatgaactt gctccgactg ttgcagaaat aaatatcata
     8821 gttttcaagc tatttgcata taaatatgct gtagctgctg gtgtaattaa cattgcaaca
     8881 attaatatag ttcccacact ttgcatagct gttactgcta ctaaagttaa tagaaccatc
     8941 aatagataat gataaaaact gacattcatc cctattgctt ttgccattaa tggatcaaaa
     9001 gaagtgatta ataattcttt aaaaaataga actactataa ccaacactaa aaccgaaacg
     9061 ccaattgtaa tccacttatc aatatcttgt acagctaaaa tatttccaaa taaaatatga
     9121 aataaatccg ttgagctatt tgcaactcct ataaggataa ctcctagtgc taaaaaagaa
     9181 ctgaaagtaa taccaattgc tgtatctcct tttattacac tattgctttt tataaaagta
     9241 ataatgatag aagctaatag accaaataca atcgcaccaa taaaaaaatt aatacctaaa
     9301 atataagaaa ttgctactcc tggcaaaaca gcatgagaaa tagcatcacc cataagagac
     9361 attcctctta aaataataaa acaacctact gttccagaaa caatccctat aactacagat
     9421 gtaattagag cattctgtaa aaagtgaaag tcatacagac cattaataaa attttgaagc
     9481 atattattcc ccctctccaa taaatatagt atctccatag gcttttttta aattttcttc
     9541 attaaataca gattctacac ttccgtgagc aattagattt cgattcaaaa gaataatatt
     9601 atcaaaatac ttctttacct tgcttaaatc atgatgcaca attagcactg tttttccctg
     9661 atgtttcaat ttttttaacg tatccataat tattcgttca ctaactaagt ctatccctac
     9721 aaatggttca tctaaaaata tgaaatccgc attttgagca agacatcttg ctaaaagtac
     9781 acgttgaaat tgtcctccag ataattctcc tatttgatga ttagaatatt ttcccatatc
     9841 gaccttttcc aaagcctttg aaactctttg ccattctgca tttttaattc tttgaaaaac
     9901 tttcattccg gcatatgttc ccatagatac acattctttt acttttattg gaaaagtaaa
     9961 atcaatatca gctttttgct ctacatatgc aatcttcacc agttttttat tgatatcttc
    10021 attattaatg gagacctctc cctgatgagg gataatattt aacattgctt taagcaatgt
    10081 ggattttcca gcaccatttg gaccaataat acctgttaaa gcaccttctt gtatttttaa
    10141 agaaatctcg ttcaatgcta aaaaatcgtt ataacaaaca ttaagctttt tcacttctaa
    10201 cataactttc tccttctaaa tgataatgat tatcacgatc atatggtata caaaaattat
    10261 attttagtca accctaaatt ttatagttta gatttttttc tgttgctgaa gattagaatg
    10321 acaaaactat ttgaaactac gcgtatagta agtagctttc aaaatatata aaaaaggagc
    10381 gttctcaaat gagaacatgc tcctaaaact actttattgg cgtatggaaa aattatactt
    10441 gaagtggata gcgggttgaa taagttgata agcatcaaaa catttataaa aattattcaa
    10501 gactatgtct gttccatata caaatatgaa aatcagtaaa ctccatttaa cttttttcgt
    10561 agtgtgtctt tttatacatg tttatcgtta ttaatagtga ataggaaaaa aactattttt
    10621 gatcattgac aattaaatac aatttttgaa taacgatccc tgctattcga gctagaaact
    10681 caagtacgcc atgacctgat tagcgacaaa tacttagaga tttctttttg ttctgggaaa
    10741 acaaaatgcc atgacaatag cagcaaataa tgatacttcc gtaaggctcg ccgtaaactg
    10801 gggagaggtg aaattcctat gtgcttaccc aaagcaattc aaaaatttca taactactat
    10861 tttaggagat gattttatag aatccagagt ctacctgaat aaaaaacaaa cctgtgcatt
    10921 tctaggaatc gataataata ctctagataa gtggattcgt atatataatt tcccagtaat
    10981 aagagttggt ggtgtagttc gctttgacgt agaagatatt aaagacttta tgaatacatt
    11041 taaaataact tgaaattttc ttctcgccgc atatactaaa attagtgaaa tatactgcct
    11101 tacttttttt aaggagtgag gattttgcct acaaagcttt caaatgggaa atataaaact
    11161 aatctgagat atccaaaaag gtttaaagaa ataacaggaa ttgcatcaga aaagtatcaa
    11221 aaaacatttc caaataggca attggcgatt aaagccgaaa atgatatgaa gaagaaaata
    11281 gaaaaagttt tgagagagga gaatgcaaac tcattagaat taaaaggaaa aataacgttt
    11341 aaaaaatttt acgaatctaa atggcttcct agatatgaat taggtcaaac aattagatca
    11401 aacaggcctc ctagtgatat aaccattagt aatacaaaag atatatttcg gttgcatatt
    11461 ttacctatgt ttggtgaata cgcaatgaat tatctaaata tcaatactga gataatatca
    11521 gatgaattaa ccaaaaaatc gaaagaatat gcaaatataa aaatcataaa aggatatgtt
    11581 cgttccatgt tcgatattgc agaaatacta aattatatcg aatttaatcg aactactaaa
    11641 attattcaaa gtatcactgc acctaaaaaa aatgctttag aagaaaaaag aatacaagaa
    11701 ggtaagcaag cactatcatc aaaagaactt actaattgga tagaagcagt aaatgatgat
    11761 tttaataatc atttacttac ttttcatgat tacacacttt ttatgattac cctgtatctt
    11821 ggtgatagaa aaagtgagac ttacgctttg caatggaaat atattgattt tgaaaaacaa
    11881 acggtccgtc ttaaacatac tttagataaa tatcaaagaa aaaagtttac aaaaggtcga
    11941 aaagataccg tcattcaagt accagaagtt gtaatgacat tgctaagtga atggaaatcg
    12001 gtacaagctg atcaattgtt gaaattaaaa attaagcaaa cgttagatca gtatctcttt
    12061 acttatacta aaccatctgg agaagtaaat tgtcctgttc atgccgatta cctaaactat
    12121 cgaatcaatt caattaaaag aaggcatcct gacttagttc atttatctcc acacaaactc
    12181 cgtcatactt atgcaactat agctagacaa ggtggagcag atatgaatca aatttcaaat
    12241 gctttaacac actcagatat ttctacaaca aagatatatg tgaacacacc agatatagtt
    12301 gataaggctg tttttgaagc ttttcagaga ggattgaata agtgtgattg atatgtgttc
    12361 ttaaatgtgt tcttaaaaac aaaagaagca ctttccaaat aattgaaaag tgccagaaac
    12421 gttgatatat aaaggatatt aacgttttga gaattgtgaa gctttacgtg ctttcttaag
    12481 acctggtttt ttacgttcga ccatacgtgc gtcacgagta agtaagccag cacgtttaag
    12541 agctaaacgg aagtcaggat caacttctag caatgcacga gcgataccat gacggatagc
    12601 tcctgattgt ccagcgtatc ctccgccgtt tacgttaaca aatacgtcat atgcgccttt
    12661 tgtttcagta acagcgaaag gttgattgat tacttcacgc aagtcagcat gtggaatgta
    12721 ttcttcaaca tcttttttat ttacagtaat tttgccagtt cctggtacta gacgtacgcg
    12781 agcaactgcg tttttacgac ggcctgtgcc gatatattga acttgtgcca atgaaattcc
    12841 ctcctattag attaagtttg tgatgtctaa aacttctggt tgttgtgctg catgtggatg
    12901 ttctgctcca ccgtatacgt tcaattttgt aaattgtttg cggcctaaag tgtttttagg
    12961 aagcatacct tttacagaag tttcgatcaa acgacgagaa tttttagcac gcaattcgcc
    13021 agcagtgatt gatttcaatc ctcctgggta gttgctgtga cggtaataaa ttttgtcagt
    13081 cgctttttta cctgttaatt tcactttgtc tgcattgatg acgatgacga aatcgccagt
    13141 atccaaatga ggtgtgaaag ttggtttatt tttaccacgt agtacagatg caacaactgt
    13201 tgaaagacgt cccaatggaa catcagttgc gtctactaca taccatttac gttctacttc
    13261 gcctggtttg gccatatatg ttgtacgcac gttattttcc tccaatttaa gttctgatgt
    13321 ttgacataca cttcgagttt ccggggctct ttgtggggca aacaatacca tttaacatat
    13381 tacccgctaa tctagtcaat gtcaacgttt ttctgcttga ttgaaaaatt tatatgaaac
    13441 tttacttctt ttctttcttt tatataaaag ggtcgtttcc tgtcaaaatg aaaaacaaaa
    13501 agaggctgtg acaaaagtct tgtcacaacc ccttattttg aataaacggt gttctcctgc
    13561 caactcctcg gtcacaagtc acaatatcca taaaacataa gaagctgttt tttaaaattg
    13621 tttcttgtga ctcggagctg aacggcaggc ttacaacctc ttggtaaacg gtgttcaatc
    13681 agctgaccct cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttgacgtttg
    13741 tttctctaat actcaggtca aaacgctgat ttcacaacct ctttttcggt caagttcaac
    13801 aacagttttt acgcgtcgat ctctggcgct ttgcccaaat gagcttggat catccagata
    13861 cgtttttctg tggctgtttt gaattcgata aagatatctt gtgttggatc gtcgccttct
    13921 tctccagaaa cttcgattcc tttttggtaa agatctgcta gataacgata gcctgcaaca
    13981 agtgtttcca aacgttctgg aatagtacga tcccagcggc caatttcatc agggattttt
    14041 gtgttatctg caaattcacg caatgtagaa taaggtgaac cgtctaatgt gatcaagcgt
    14101 tcggatactt catctaactg agcgttcaaa tcatccatga actcatccat cattgggtgc
    14161 aatgtaagga aacctggtcc tctcatgtac caatgtgttt ggtgaacaac aacagacatt
    14221 tggcttagat ctgcaactaa ttgatttaat acttctttgg tttgttggaa tttcatcttt
    14281 cttgcctcct aacattttct tacacttaaa ataatagtag actttcaatg aaaagtacaa
    14341 caaaatcatt tacctgagaa cgattttcag ttcgcttttt tatccgtaaa gccaatcatt
    14401 tttcagaatc agctaattct tctcgcagtt gagcaaattg ctgataagtc tgtgctaaat
    14461 gcttttgtgt cgaaacaaat aatggataga ttttctgata ttttttatga tcagtttctt
    14521 gtactccaac ttgctctttc aattctatca attcctgtgc ttcactgata ctttggatct
    14581 ttccgatgct tctccagcct aaaatcacag ctcctaatgc ggatgcttct gtgacagtag
    14641 ggaaaacaag cgggtaaccc agtacatcag ccatcatctg acgaaaaatc ggagattgag
    14701 caaacccgcc tgtagcacgg atctctgtga gctgattttc ctggcaaact ccttttaaaa
    14761 tccgataaag gttgagacag atcccttcta gaatcgcctt catcatttct ttttctgtat
    14821 gttgccgctg taaaccgata aaactgccaa acgcatctgc gtcccaaagc ggtgctcgct
    14881 caccaagtaa atagggatga aaaatcagac cattggcacc cgctggcaca aaagcgatag
    14941 ccgaaagcag tgcatcatat ggattttctc ctttttcttt agcagattga acgacttcct
    15001 gtaaaaatgt atgccctgcc caatcaaaga tgccagcacc attgctagtc gctcctccca
    15061 ctacccaatg tttctgatcc agaacataac aaaacgtctc catttctgga tggaactgag
    15121 gctgagtagt aataaaacga agtgctccgc tcgttcctac ggtcaaagcg gcgatcgtat
    15181 caccaaaagc acctaaccct agattagaaa gcggcccgtc tgcccctccc aaaacaaatg
    15241 atatatcatt tggcaaacct agttctgctg ctgtttttga tggtagacct gttacttgat
    15301 aggtggaagg aaagacttgc ggcaattgct ctattcgaat gttcaagtag tcaagcactt
    15361 cttctgccca ttggaattca tggatatcaa aatatccagt tcctgaagct gttccgtagt
    15421 cgctgatcag ttcgccaaac aatctataaa agatatagtc tttgataccg acaaaataag
    15481 cagttttttt gaataattca ggttggctgt tttgcagcca cttgattttg ttcaatggtg
    15541 tcattggatg aatcggcgtc ccagtctgcc gataaaattc tttgccaagc acggtatatt
    15601 tcagtttttc tgcttcgtcc gctgctcggt tatctgccca agtgatcatt cgagataacg
    15661 gtgttttctc ttcatccaac aatatcaggc catgcatcgc agaggagaag gagacaaatt
    15721 gaatgattgt gtctgaaggc aactgtttaa ttacttcttt tataacggtg cagacacctt
    15781 gaaagatcaa ttcctgatct tgttctgcca tcccttgtgt ttcttggatc aaagggtagc
    15841 gaaaataagc ggatgctgct tcttgggctt catctgtgaa agcaactgct ttggtttgtg
    15901 tggttccgat atctaatccg attgaaacgt tcattttttc acctcaaatt ttatgtatgt
    15961 acaaaaattt agtttaagct tgcgtatctt ggttttgaaa agggggagat ggcgtgttac
    16021 tttattttat cattggttgc tgtatcggat catttttatg tttaaccgcc cagcggattc
    16081 cgttaggtca ctctattatt tatcctagat ctcattgtgt caaatgctgt cgttcgcttt
    16141 cttggtatga attgattccg attctctcga tcatcgtcca acgatttcgc tgccgctatt
    16201 gcaggtgtcg gttgcccttc tactatttgt tggcagaagc cttatgtggt ggtctttttg
    16261 cctggttctt tactttttct tccgcaagaa atttttcgac gttcatatgg atgctttcgg
    16321 ctttgctatt ttcgttgatg gatttatttt acttcatggt ccattctcac acgctattct
    16381 gttcttgggc aattttatgg attttttggt tgcaggccgg cgtcttccaa tggcaaagtg
    16441 tgctgttaac gtttatcgta ggcggtgtgt gccttcgcta tggtcaatcc tatctcggag
    16501 ctggcgatac tctgttgatg ctttcttgga gtggtggctt gtctctagaa gagttgcttc
    16561 agatcttgtt tcttgctagt ctgtttggaa tatccgtttt ttctgtttac tttatctgtc
    16621 ataaaaaaaa gttggaaaag ctgcctttca ttcctttttt gagcagtgcg ttgctgattg
    16681 tccttcatct taggtattga aaaaaggttg tgacaaacga cttgtcacaa cctctgatat
    16741 atagtgttct cttaccaaat ccttgtgact tggagctagg cgacaggttc acaacctctc
    16801 cactaaatgg tgttcaatca gctgaccctc agtattagct caaaatgctg gttttacaac
    16861 ctctgatata cggtgttcca taagtagctt tttcggcttc ttatttcttg acgccccaat
    16921 gtcgacgaag gaaacctgca cgtccagaac cttctcgata aagattgtaa tggagcttgt
    16981 ttttcttgta ataatcctga tggtaatctt ctgcaggata aaattcgctt cttggttcga
    17041 tttttgtcac gattggctta tcgaatcgcc cgctgtcttg caatgcttgt tttgaacgct
    17101 ctgcaatatt tcgttcttct tcatcattat agtagatcac tggacgataa gaatcaccgc
    17161 gatcagcgaa ctgtcctaat gcgtcagtcg gatcggtttg ctgccaatag atttccacca
    17221 actgatcata agaaataact tctggatcat attcgatctc tactgcttct gtatgccctg
    17281 ttgtgcctgt tgttacttgt tcataagttg gatttggtac atgcccgcca gtataaccgg
    17341 atgtcacaga aatgatccct ggctgctctt caaatggttt gaccatacac caaaagcatc
    17401 cgcctgcgaa aactgctttt tttgtcatat aaaatccccc ttttttctca tcataccttg
    17461 atttccaaaa ggaggataac gttttgcttg tgacagaaaa aacggaaact attatatacg
    17521 tgatacggct tctgataaat gccaaaacga acgtacttga tcaaaaacgg ttaaatcagg
    17581 aatcacatat tcagcagaag gaagatttat gttttcacct attcccaata catgcatgtt
    17641 accagccaaa gctgcttgac aaccagcttc tgaatcttca atgaccaagc atgcatttgg
    17701 cggtacattc aattgctgtg ctcctttgag aaaaacttct ggatcaggtt ttgcttttga
    17761 tacttgcgtg ccgtcaataa ggacatcaaa ataacttgtc aaattcgttt tttctaaaat
    17821 gaggcgtgca tttttgcttg ctgatcccaa tccgatcttg atgtttgttt ttttcaagta
    17881 ctcaagaaca gctattgcac caggcaaaac ggatgtttca tccatttttt gaatcgcttg
    17941 aagatataga ttgttttttt gctcagcaaa ggctgatttc tctttttctg agaatttctg
    18001 ttccattttt ccaaatgaca gcaatcgctc tagtgactcg gttctggata ttcctttcag
    18061 ttgctcgttt tctttctcag tgaatggaat ggacagctgg ttagctaaaa ccagccacgc
    18121 ttcgtaatga taacgagctg tgtctacaag aacgccatct aaatcgaata gtgctgcttt
    18181 gattttcatc gattacctcc ttgaaaatga acgacttgaa gtaaagatgc gatgttttta
    18241 gctgtgcgat gaagattatc agtggcttgc tgtaaagctt cttgtaaagg cacagctgca
    18301 ggaaggatcg gaaaaacggc cgaaatgcca acatctgctg ctttttcgct tcctttacca
    18361 acacttccgc aaatagctat cacaggaacg ccctctggta ctcttctagc aatcccaact
    18421 ggcgcttttc cttttagact ttgtccatct agacgtcctt cgcctacgat tacgaggtct
    18481 gcttgctgta cccgtttatc gaactccagt aaatccaata cgtagtcgat gccagagact
    18541 attttccctg aagcaaatgc taacagtcct gcacccattc caccaccagc actactgccc
    18601 gatgcctcga ttacttgagg ggcaaatctc atataaaaat catgcatggc ttggtcagcc
    18661 actgctaatt cattttcttt caagcctttt tgtggtccga aaacaaaaga agcgccttct
    18721 tttccgcata aaagattagt aacatcagta atgacagtga ttttggcttt aatcagctca
    18781 ataggcacgt tcgttttatc aactgtagca attttaccta gattttctcc cagtgcttct
    18841 actttttgat tatcttgatc gtaaaaagca taacctagac cttctgccat gccgatgccg
    18901 ccatcgttgg tactacttcc gccaacgccg atgatgatgt gtcgaacacc ttgttgcaac
    18961 aattgtcgta tcagttctcc tactcctcgc gtggataacc taagtggctg ttgttggttt
    19021 agtgggacat ctttcaatcc gacgatttct gccatttcaa aaatcgcagt ttgaccgtct
    19081 gaagcgtact ggatttcttt gtttttgcca aatgcattag taacgactgc tgtttgccgc
    19141 ttcaacctta aactgtctgc taatgatgaa accgttcctt ctcctccatc gcctataggt
    19201 agcagatcat attcagcctg aacaaaaact tcttgaaaac ctgattgaat ggcttgtgct
    19261 gcctctaagg ctgtcagact ttctttgaaa gaatctggcg caatcagaat acgcattatt
    19321 ttcccctccg ttccaagatc agttcttttt ccagctggta ttggtctcca ttgacaaaaa
    19381 taggtaatgt ttctttcgtc tcttttttta ttactatttg tttttttgtc aaaacaaata
    19441 gcaacctgtg atttttccaa ataaaaggaa aagaaatttt ttcccatgct gaaggaagtc
    19501 ttggagaaat agaaagagtt ccattcgtta ctgtaatacc tgaaaaacca aatataactg
    19561 ctagccatag tgctcctaat gaagcagcat gaattccgtc atctgatgaa tgcggatctg
    19621 gtcctaaatc aatcaggcac gcttcttgga ataaatgata cgcccattcc atttcttgga
    19681 tacggcaagc ctctatggcg tgaatcgctt tactcaacga tgagtcgtgg attgttttgt
    19741 tttcatagta atgcaaattc ttagaaacga ttggttgaga aaaatactca ggtaataagt
    19801 ataacagcat gacaagatct gcttgcttgc tgacttggag ttcatttact tcgctacggg
    19861 cataatcaag taaaatagac tggctgccag gacgttcttt gtaagtggtc aaatcaataa
    19921 ctggcttact gagatacgtg tcatcttgcg gaatcagctg ttctttgtta ggcactggca
    19981 aatataactt atctaaaaaa ttttgacatt ctttcaaaaa aagcggatcg ccagcttgat
    20041 atttaacagc taaggccaca ttataatgcg ccagatagtt cgtatatgca ttattgtcga
    20101 tatattctgt gtattcatct ggaccaatga ctctgtgtat ctctaaatga tcatcgactt
    20161 ttacagctct gctcagccaa aaagctgctg tttcatgaat gagcgttttt ccctttgttt
    20221 ctaaaaacga ttgatcctct gttgcttgga catactcttg aacggcataa gcaaggtctg
    20281 cactgatatg atgttctgcg gcagctgaag caacttcttg tcttgttccg gttttgatat
    20341 tcaatgcagc aaatttgggt gtctcttcct gaccagtgtg tgcgctttcc catgggaaaa
    20401 gtgcaccttg atagtgataa tctcttgctt tttgccgtgc gccttctaat cgatcataac
    20461 ggtacagcag caaattcttt gctacttcag gaaactgata taaaaagaaa ggcaggataa
    20521 aaatttccgt atcccagaaa acatgccctt tataaccttc gccagttagt cccttcgccc
    20581 ctacactaac acttggatca ttgcggggag tcataatctg taaatggtaa gcagcgaagt
    20641 ccatcgctaa ctgatcgaaa gaagaatcgc cttggatcgt tatccgatgc tgatgccaaa
    20701 aatctgtcca ataagacatc gactgctcta aaatttctgt ataactaacg cttttctggg
    20761 cttgggcaat acttttctta aacgggtctg tttgctgaag agaggtagag agcacgatga
    20821 tcttctccag cgtaaaggac gtattttttc tgatcttttc tgaaaaggaa gcggtgatcc
    20881 ggcgattttt tgcttggaat acacctgctt tattgaattt agctgcaatc ccaatatcaa
    20941 ttttactttc tgttgtctgg taggatgcac tgatcaaatc gtcaccaaat actcgcagtt
    21001 ctttttcaac caactgctgt gtaccaaaat tggtttgctg tccatcgata cccgttttca
    21061 catctacttg gcagtcttcg gtcaacggct tgatgaccac tttcagggct gcctgatgga
    21121 gattattttg atcagcaaca cgacaagtaa tgacttccac tttttttcct tgagcagttt
    21181 caatgagcgc atagcgttta agctctccca tttctaaatc tagtgcccgc tcgtatgcaa
    21241 gaactttcat cgtctcaaga gaaaaatgtt caccgtcgat ttttatttcc catctgatga
    21301 catctgggag attcactaac tcggctgatt ctgctcctaa aggtcggtta tatacaccag
    21361 ctgcaaacat tccccttact tgattccgat agtcttcctc ctggcttcct ctgataccaa
    21421 aaaatccatt tccaatggtc atctgactcg catacttatt gatatactct aaatcaaact
    21481 gtttttcttc tactttcata aggtaataac ttctcctgtt tctgccgaac gatataaatt
    21541 ttccaccaga ttctggatga catatccttg ccagccatct gcaatcatct gagtctggtc
    21601 accgagcgcc cgattcacaa aatgagccat acttagttca tgtgggtcat taactggcgt
    21661 ggtcttttgt aaggaaacca gctttccatc tacatcttcg aatagttcgc ctggatacag
    21721 attagcacct gcctgatcgc cacaaaaagc cacattcagt ttgtcttctc tgatattcaa
    21781 agcaaacgaa gtattcagtt ggatgatctt gccgttttct aattccagtg tgccaaaaat
    21841 tgcatcttct actgtgaatt tgcttggatc ccatttcccg aatttccctg aatttttttt
    21901 cgtaccaatt ttttggaagc tgtacgcccg aacgcttttt actttaggaa atccagtaat
    21961 atacatcgct gtatcaagca tgtgaatgcc ataatcgatc aaaggcccgc ctccttgaag
    22021 ttctttgtcg ataaacgttc cccagcctgg aacaccagac cgacgaagcg cattcgcttc
    22081 tacgtaataa atatccccga gaacatcaag atgattcttg atcattgttg cttcgtcaga
    22141 aaaacgatgc tggaatccat aagccaaaat ctgcccgttt ttatcggctg tctctgccat
    22201 ctttcttgct tgatcagctg tcatagctgg tggtttctcg cacatcactg aagcattagc
    22261 ttcgagggca gccaatacat gttcgaagtg aaagcgattg ggtgtacaga cactcacgat
    22321 atcgggagaa atctcattca gcatttgtgt aactgatgta tacgcatgag aaaatcctgt
    22381 acgtcggcaa aagtcttctg ctcgctggct gactggatca accacagcgg ccaattctaa
    22441 gacatctgat cttttttgat aataagcagc atgtactttt tctgctactt gtccagcgcc
    22501 tatgattgct actttcttca tttatttctc tctcctttgc ttatttactt attgttattc
    22561 tttctctatt ctttctctaa atcatttaaa tgacgcgctg tttccagaca gtttttcaag
    22621 tattgcaatg attcctgata aattttcact tcattttctc ctcgcaaacg acattcatat
    22681 accatataac cttcatactc attttgaagt agcgtttgaa aatggcgagt aaaatcaatt
    22741 gcgccagatc ctggttggta gcgatgatta tctgctaaat ggatatgacc cacatactct
    22801 ttttgcgtag tcaatgcttt tgaaatatca tcttcttcaa tattcatatg ataaaaatca
    22861 gcgatgatct gcacatgttt caattgatta gagtcaatgt aatgacgcgc atccgctaac
    22921 aaattgatca tatggtcttg gtatcggttc aaaggctcca aataaataac cgtacctgtt
    22981 tcttcggcta ctttatctaa ataacgaagt gattcgctga ctgctttata gtctccttct
    23041 ttgcttcggg gtgaaaccat cggcggtaat cgataagtga acattcccca agctgctggg
    23101 ataacgattc ctttgcctcc aacctcgctt agtgcacgca aaatctcagc aatttgacgt
    23161 acaccattaa gacgccgttc ttcgataaag tcaccgatcc acccatcgta gccgttacaa
    23221 gctgtcacta ctgggatccc tgtttctttg attgcctgtt tgactgtgtc gatattttct
    23281 attaacaact taccgtcgat ttcgtattct tcaaatccac agtctttgat aaatctaaat
    23341 ttttctaaaa tgtctcttgg gaaaaatgct tgatcttgcg taccgaattt cattgttctc
    23401 ctcctcggac ttgaacgccc attttgatac tttcttctgg gtgctggtct acataacgca
    23461 tgtaagcttc tgcgctttct gcaaatggca caactggatc aatgatttct tcacaatcca
    23521 agtaaccgtt catcaataat tcccaacatg tgtcctcgat tcgtttacgg ttccatcttg
    23581 ggtaatctgg gtttggttca ctagatgctc gagcaaagac gatttttgca ttattgaaat
    23641 gtgcttcgcg accaagatta aacccagctg gaaaaggttt agcaaaggca acataagaaa
    23701 tgatcccgcc atagccgatc cctattaaag aagcttgtaa tgcggcagca ctgccactcg
    23761 tttcaataac tgaatctact ccacgtttat ctgtcaattc ctttagacga aaaccgacat
    23821 ctgctttcat aggatctagc gtgtaatcgg caccgtgttt ttctgctaat tctctacgct
    23881 gcgctatggg atcgatccca acgatggtag tagcacctgc ttttttagct aattgaatcg
    23941 caatttgccc gatagcgcct aaaccgaaga ttgctacagc atcacctgct cgaacatgtg
    24001 cttcccggat accacctaaa gcgaattgag caggatcgta acatactgca tttttccatt
    24061 gatccaaccg atcaagttta cgtagtttat aattatcaac accgtttgta atcagcgttt
    24121 cacgaatggg tccataagtg cagactaact ctcctttttg atactctgtc accaaactgc
    24181 ccgtttcttc gatcgtaccg acaatcatat ttcctaattg aaattcgcca aataccacac
    24241 cgcgttggct tccttcttct cttggcaaga ataggcgcca ttcttctgaa aattcttcat
    24301 cgataaatgg acttaatcct ctaaagtcga caatttctgt tcctggtttt ggagtagcga
    24361 aaattactcg gatacgtact tcgttctttt cgatctcacg gtcttcgtaa tctttgaatc
    24421 cggctattct tggttctaat gcaactaatt ttttcatctt ttctttcctc tttcttttct
    24481 ttttatcctt ttacgccgcc ttcgactaaa tcacctttaa taaatcgttc agataacgca
    24541 tacataatga tgactggtaa agcggtcacc aatgaagcgg ccatcattcg tccccaaatg
    24601 tagtctggtg tactgaataa tgtgttcaaa ccgattggta aagtaaattt ttcggaggtc
    24661 gacaggaaaa tcgaagcaaa taaataatca ttccacgaaa ccataaagca ataaacgaag
    24721 acagaaatga ttccagaaat agccaatggt ataatgatcc gaataatgat ctggatccgg
    24781 ctaagaccat cgatcattgc tgcttcttca atatcagtcg gaatcgtgtc aaaataactt
    24841 tttagcatga atatcgcagt aggaagtgtt tgaacgacca tcgtgataat cagtgctgta
    24901 ttggtatcat acaaacctag accggaaatg attttaaata atggaacaac gatcaaaata
    24961 ccggaaaaca tataaacggt gtaaaagcta gcattgattt tcgagcgagc tttgaagcgt
    25021 agttttgaca aagcgtatgc cccgctgatc ccaatcacta ctgcagttac ggaggctatc
    25081 aaggagatca ctaaactatt tttgaaatac atgatgaatg gaaaaatctc agcattgaaa
    25141 atatcctgat aatgttcaaa agtccattct ttcggaaaaa tagtttgtgt cgttgcaatt
    25201 gcttcttgtg aatccttgaa tgatgtcatc agcatgatca aaaatggaaa caaattgaca
    25261 gctaatagca acaaaatcgt cgcgtaaaat aagattttcc tgctcttttt atttttcatt
    25321 tcaagtccac cttctttcgt gagagcaaga cgaccgctgc aataatgatg aacaatagaa
    25381 tcgatatagc tgctgctttt ccaagatcat tgaacgcaaa ggctgtctga tacaaataaa
    25441 cgcctaagat attgacttta ttcgtcagta gatagacatc tgtaaacata tagaacatcc
    25501 agatagatcg taaaacaatc acagttgcta gtactggttt gattgccggc agtgtcacga
    25561 tcttgaactt ctgccaagca tttgcaccgt ccatttcagc tgcttcataa agcgttttgt
    25621 cgactgtttg caaaatagcc aaaaatgaaa taaacgcata cggatagtat ctccagatag
    25681 caaacaaaac gaccataaag aagctagcaa ctggctggtc gaaccacagc ggtgcttcgt
    25741 tgaaaagctg caacctatct actaacagat cattgacgat tccataacta ttattgaaca
    25801 tatatttcca agcaaaaatc aaagaaatag atggcgtaac ataacttaat aaaatcagtg
    25861 aacgcaccgt tttcctaaat ctgaacttgc ggttaagcaa aatagctacg gctaatccta
    25921 aagctgtact gcctgctaca actaagaatg tgtaagcaac tgttaaacct aaagaacgat
    25981 aaaattcact atcacttaaa atttcaagat aattgcttaa tccgataaaa acggcgtctg
    26041 agttaggatt cagcggcata tcgaaaaaac tgatctgaat attggaaagc attggaatca
    26101 gcactaaaac agctaataaa agcatgcttg gtgctaaaag tcccatggct aattttaagt
    26161 cagaatgttt ccttggacga ttcattgaaa cctccccctg ataatctgcc aatagctatc
    26221 tacttttttc gaacaccatt ggcagaactt cttgttattt cgtatttttt tgtgcgtcag
    26281 ctaaagcttg atcgacatct tcgtttccga cagttacttg gttcactgct tttccgatca
    26341 cacctgagct tgtgatatct cccattttcg tgaaattctt ttccccgact agtccaaaga
    26401 cttgtacttc atcaaaagca ctagcaatct cagatggcag ctcgccaaaa gacttgatga
    26461 cttcattttc tttgtaacca tccagttcga ctacttgttt attcactggt tgagcaccac
    26521 caggagacat caagacccac ttttccatgt ttttcggttg agataagaaa gctgtgaatg
    26581 cttcagcagc ctctttttgt ttctcgtcta gaccagaaga aatcgtcaat ccagatactg
    26641 tgccatagac agcttgatct ttttgtgttg ggatagcaaa accgatattt tctgagttcc
    26701 ccgcttcata aacagaaggc aaaatgtaag ttgaatagat tgccattggt gctgttccat
    26761 tcatgaaagc atctttgatt tctgtcacat cattagagcc tggcatcgtg tattgggata
    26821 agttttgata aaacgacaat gcttctctca tcttttccgt atcgatcgtt acctcgcctt
    26881 catcatccaa aacatttgct ccatttgaca aagcaaattg agagaacgct tgctcactca
    26941 ttgttccttc agcagtaggc atagcaattc catattgttt attctcttta tctgtgaaat
    27001 gttgagcaat ttttaaaata tcatcccaat tttctggttc tgaaaaacca gcttctgata
    27061 atttttcttt gtcataccaa ataccttgaa cccagccgct aattggtgca gcaatatagc
    27121 tttttccatc ttctgaacga acaaggtttt tggctccgtc ataataatta tcttctccag
    27181 cttcctcgat aacgttttgg actgccgtct gatcaatcag ttcatcttta tccatcactt
    27241 tagcaaaatc ctgactgact tccattactt ctggtaattt accggaacga gctaacgtaa
    27301 cgatttttgt attgaacgct gattcttcta ccggcacttg cttgattttg atgtctggat
    27361 tttctttttc aaaatcagca accagttcat tgatcacact taatcgttcc tgttcgacag
    27421 aagagtgcat gaattgaatc gttgtctgtt gtgattcgtt agagctttga ctttcaccac
    27481 aagcagtaag aagaaatcca gtagctagca gacaggtagt aacaataatt tttttcattt
    27541 ttttccacct cttattttat tattttcagc cacatatatt catacggctg gaatatcaac
    27601 tttcgttctt gactgcaatg gataatttgt ccagaaaaca ggtctaggta atctcctttt
    27661 ggtaaaagat aacctgctga ttgattggaa aaattatgga taactaagac tttctcttcc
    27721 ttcgcttcgc gaataaaact gataaaagaa tctgctgagt ccagtaagta aatcaaacta
    27781 ttcggatgga aggctttttc tttttttcga atagatagca tctgggtcaa ttcaccatat
    27841 actgctgttc ttaatggatc tgtctgcaac tctttctcaa tatctgctaa caaaaatttt
    27901 tgccgattga tttttcgatt ctctcctgtt ttttctacgc ctttgtaatc atttcgactt
    27961 cccagcacac tttgaatata aattgccggc acgcctggta acgttaccaa aaatgaatga
    28021 gcagctaaaa agcgtttgct tctccattcc gtcgtatctg ttggagaact taacgcatct
    28081 agatacgtag cgtttatctc atatggactg gtcgttccat ctgggttttt cttgtagttt
    28141 actcgggcac cctctttctt cagctgttcc actaattcaa ggatcgtttt ttccggcaga
    28201 atacctcgta ccggattcaa tccgataccg tcatgggagg ctaaaaaatt aaaaaaagtt
    28261 gtttttttcg atgcgaaatc gatggttttt aaccattctt tcaacttact tgtattaccc
    28321 gaacgaatcg cttgaagggt caaaggtggt agcgaaaact gatagaccat ctgtgcttca
    28381 ttctcgccat tcccaaaata actgatatta tctttgtgtg gcacatttgt ctcagtgatc
    28441 aataccgttc ctggagctgc ttcatcagcg attgcacgga ataattggat gattttatgc
    28501 gtttctggca ggtgtataca agaagttcct agttctttcc ataaaaatcc cacagcgtcc
    28561 aagcgaataa atgaagcgcc tttttctaag taaaacaaaa gaatatcgat cattttcaat
    28621 aaaacttctg gatttttaaa attcaagtct acttgatctt cgctaaaagt cgtccatacg
    28681 tgtttttctt tcccgttttt gtcaatgaac gttgtaagta acgggctggt ccttggtcta
    28741 gtcacgctgc ttgtatctgt atccagtggc aaatcaataa aaaaatcttt gtacgtatca
    28801 ttcaagttta aatattcctg aaaccaagta ctttcggcag acatatgatt gcagacaaag
    28861 tcgaacatta accgtgtttc gcttctcatt tcttctatat cttcccatgt gcccaaacgg
    28921 tcgttgattg cctgataatt tacaacggag aaaccatcat cagatgtata tgggaaaaat
    28981 ggcaagaaat gtacaactct aaatgtatct gccaaatact tttgataaaa tttcttaaat
    29041 gtacgcaaag ttggttgctg ctcttcaaaa aattgatccc cataagtaat caagaaaata
    29101 tcttcttcgt cccaatgcag tttgcctgcc tggcctttct tctgctgata aacactgact
    29161 ttttgttcaa tcatagaggc ggttttctca gacaaatctg agtcgcgata aatcgcttgt
    29221 aaaagttttg tatacatcgg attccccttt cttttttggg agcgttccca acgatttgat
    29281 catattatgg aaaacgctta ctgtcaatag cattttttta aataattttg atatacacaa
    29341 aaatacaaat aagaaagctt atgtatcaat aattaatagc ttcttaaaaa aatatggtct
    29401 ctcatgatca tgcaagctat tcctaaattt tcaaaatcat agacaaaaaa gaatgtctat
    29461 tgtactatgg aattattcta aaacgctccc aaaaaggagg tgcatataaa attgaaacct
    29521 actatttatg acattgcgaa aaaagctaat gtctcaaaat caactgtctc cagagtatta
    29581 aataaccagc caaatatctc tgataaagca aaagcaagtg tacttcaagc aatcaaagaa
    29641 ttgaattatg aaccgagcaa aattgcaaga ggactgtcag gaaatggttt tgacgcgatt
    29701 ctagcattat ctcatcgtac ttctcatacg acctatggca atcctttttt ttcagatgtt
    29761 attcaatcca tttctgccgt ttctgaacaa aataacttcg acttgattct acaaacggca
    29821 aaaacaccag aagaagagct agaaaaatgc gcgaaaaaga tccaagaaaa aatgatccga
    29881 ggaattatta ttttaagctc tacgatggat gaaacacttc tttttgaatt ggatcaatat
    29941 aatattccta ttgtggtaat cggaaaagtc ggaaacgact acacgaacat ctactcagtg
    30001 gacacggata atttccagga tagttacgat ttggtggaaa cactgatcaa gaatggacac
    30061 cgtcatatcg gctgtctcca tgctccgaca gaagtttatg tatccaatga tcgtctcaat
    30121 ggttaccgtc aatgcttatt tgatcaccac ctagatatcc gtaatgaatg gattatagac
    30181 ggcggctttc gcttagaaga tagcatggca gcaatgaatg atttaatgaa gctacgagaa
    30241 agaccaactg cgattttttc taccgatgca ttaaaatcat tgagtattta caaagtggca
    30301 gaacaatctg gaaagcagat acctgatgat ctttcgatcg caggattcag tgatccctta
    30361 ttgtcctccc tattttcgcc acagttgact cggatagaca ttccgacaaa tcagttagga
    30421 atcaaagcca cagaactttt attccaacta atccaccacc aataccctga agaaaaaaga
    30481 atcattcttt caaccgagat tactctcaca gattcgatcc aacaacaaga aatgtagaac
    30541 agaattataa aagaacagaa acactgagga taagtcagtt actctgttct tttttcaatc
    30601 actcatccct cgttaccccc tcccggtatt ttagcgaatt tttcttattt tccatctgtt
    30661 ttagatagct gtggataact ataatgtttg tgttaaacta ttttttccac agggaagaaa
    30721 aaactatttt taaccacgtt gtgtataagt ttttatagtt cgttttttta aacacattta
    30781 cccttatttc aatccatgtt atttggggat acttatctct aaaacataaa tagaataaca
    30841 gacatgtcta ctggttttgt tttattcgag gaattattca ataatagcta gcaaaatatt
    30901 aaaaataacc gctctatctt cccatcaatt tattttgcat tcaaaaaatg atctatcttt
    30961 acttttgttg attgacttct cttggaagat gatcgttccg ataccttaat tggatacgaa
    31021 ccgatgttgt taagatcctt tcccactttt cttctagtta gacaaattat tttagacaaa
    31081 ccaaaaaaga ggttgtgaca ataatttgtc acaacctctt ttttaaaata tctattttgt
    31141 ttgattgttc attgcgtctt ctgcagccat ttgcgcttca atatctgaaa tactgtagac
    31201 attttcgctt gctacaccga cttcttttgg ttccatgtca cgataacgtt gcataccagt
    31261 accagctggg atgatcttac ctataataac attttctttc aaaccaagaa gaggatcttt
    31321 cttaccgcgg atcgcagcat ccgtcaagac acgagttgtt tcttggaatg atgcagcaga
    31381 taagaaacta tttgtttcta atgatgcttt tgtgatacct agtaagacag gtcgagaagt
    31441 tgcaggaacg cctcctgaga ctagcgtatt gtagttttga tctttaaagt ctgcgatgtc
    31501 taataatgta cctggtaaga tatctgtatc acctggatcc attacacgaa ctttacgcag
    31561 catttgacga accattactt cgatatgctt gtcgccgatt tctacccctt gcatacggta
    31621 aacacgttgt acttcacgaa gcaagtagtt ttcaacagat aatacatcgc gaacttgcaa
    31681 caattgtttt ggatcgattg atccttctgt caatggtgca ccacggtgga tcatatcgcc
    31741 ttctgctact ttcatacgtg ctgtataagg aacagtatac gtacgtgtat ctgtttttcc
    31801 tttgattgtt acttctttgg tacgtgttgc aggatcttct gagatatcga tgacatcccc
    31861 tgttacttct gtgatcactg cttgcccttt cggattacgt gcttcaaaga tttcttggac
    31921 acgaggaaga ccttgagtga tatcgtctcc ggcaacccca cctgtatgga atgtacgcat
    31981 cgtcaactga gtaccaggtt caccgattga ttgagcggcg atcgtaccaa ctgcttctcc
    32041 gacttcaacg tcagagccag ttgcaaggtt gcgtccgtaa caatgtttgc agacaccatg
    32101 ttttgtatta catgtgaaga cagaacggat tgtcactgtt tcgacacctg catcaacgat
    32161 ttgttttgcg atgtcttcgg aaatcaattg gtttcctgca atgatcaatt catttgtttc
    32221 aggatgacgt acttctttac gggtgtaacg tcccactaaa cgatcttcta atgattcgat
    32281 gatttcattt ccctcgcgga tggcttggat ttcaagacct cggtcagtac cgcaatcttc
    32341 ttcacggatg attacatctt gggccacgtc aaccagacga cgagtcaagt aacctgaatc
    32401 ggcagtcttc aaggctgtat cggtcatccc tttacgagcc ccgtgagttg agataaacat
    32461 ttccaagacg gatagtcctt cacggaagtt cgaaatgatt ggcaattcca tgattcgacc
    32521 attaggagcg gccatcaaac cacgcattcc ggcaagctgt gtaaagttgg aaatgttacc
    32581 acgagctcca gaatcagaca tcatgaagat tggatttttc gcttccaaac cttccatcag
    32641 tttttgttgg atgctgtctt ttgcagcatt ccaaacagca atgacacgtt cataacgttc
    32701 gtcatctgtg atcagaccac gacggaattg ttttgtaata gtttcaactt gtttatgggc
    32761 attttcaatg atttcttgtt tttcattcag tacactgatg tcagcgatcc ctactgtgat
    32821 accggcatgt gtcgaatgtt tgtaccctaa gtctttcata cggtcaagca tcttagaagt
    32881 ttctgttact ttgaatcgtt tgaagacttc tgcgatgatg tttcctaagt ttttcttctt
    32941 gaacggtaat actaattctt gctctttgat atgtgcagga atatcagtac cagcttcaac
    33001 aaagtatttg tccggtgttt ggacggtcaa gttgaagtct gttggttcat tcagataagg
    33061 gaattcagga ggcatgatct cgttgaagat gatcttacca actgttgtga tcatcgtacg
    33121 ttctttttgc cattcagtaa atggtttttc acctaatgag ttagggttca caccgatacg
    33181 tgaatgtaaa tgaacatatc cgttgcgcca tgcgataacg gcttcgtttt gatctctgaa
    33241 gaccattcct tcgccttcgc gtccttcttc ttccattgtc aagtagtagt tacctagaac
    33301 catatcttga gaaggagtaa caactggttt accatcttta gggttcaaga tgttttgagc
    33361 agctaacatc aacatacgtg cttctgcttg tgcttcttca ttcaatggaa cgtggacagc
    33421 catttggtct ccatcgaaat cggcattata agcttcacat acaagtggat gcaaacggat
    33481 tgcgcgtcct tgtactaaaa ctggttcgaa tgcttggata ccaagtcggt gaagcgtagg
    33541 tgcccggttc agcaatactg ggtgttcctt gattacgtct tctaaaacat cccatacttc
    33601 gtcttcttga cgttcgattt tacgtttcgc atttttgatg tttgaagcta gttcgcgttg
    33661 aacaagctca cgcatcacga atggtttgaa taattcaatg gccatttctt ttggaagtcc
    33721 acattggtac atcttcaaga aaggtccaac aacgataacg gaacgacctg agtagtcgac
    33781 acgtttacct agcaagtttt gacggaaacg accttgtttc cctttcaaca tatgagaaag
    33841 agatttcaat gggcggttac ctggtccagt aactggacgt ccgcgacgac cgttatcgat
    33901 caatgcatct actgcttctt gcagcatacg tttttcattt tgaacgatga ttccaggtgc
    33961 attcaagtct aacaaacgtt tcaaacggtt attacggttg attacacgac ggtaaagatc
    34021 attcaagtca cttgttgcaa aacgtccacc ttctaattga accattgggc gtaaatctgg
    34081 tggaataaca gggataacgt ccataaccat ccagcttggt tgatttcctg aagcgcggaa
    34141 tgcttctaaa atatccaaac ggcggattgc acgtgtacgt ttttgtcctt gtgcagattt
    34201 caattcttct ttcaattcgg ctgcttctgc atctagatca acattatcaa gtaattgttt
    34261 aatcgcttct gcacccatag cagcttggaa ttcttgtcca tattgttcac gtttttcgcg
    34321 atattcgcgt tctgtcaaca attgtttttt ctctaaattc gtatttcctg gttcgatgac
    34381 tacatatgaa gcaaaataaa tgatttcttc caaagcgcgt gggctcatgt ctaatacaag
    34441 acccatacgt gaaggaatcc ctttaaagta ccagatatgt gaaacaggag ctgctaattc
    34501 aatatgtccc atgcgttcac ggcgaacttt cgaacgagta acttccactc cgcatcgatc
    34561 acaaacgatt cctttataac ggatacgttt gtattttccg caggcacatt cccagtcttt
    34621 tgtaggacca aaaatccgct cacagaacaa cccttcacgt tcaggtttta aggtacgata
    34681 gttaatcgtc tcaggttttt ttacttcacc gtaagaccag cttctgattt tttcgggaga
    34741 agccaaacct atttgcatac tttcgaattt atttacatcg atcaaaaggg gttccctcca
    34801 tttcatttaa tggcaaaccg caagacggct gatcttcgac cgccttgcag ctcatagacc
    34861 ggattagtct aaattatctt ctgcggtttc aaagttttgg atgacatcgt ctttttcatc
    34921 ttccacttgt tcagcagctt tcttttctaa ttctttggca gtttgctgtt cggcaaattt
    34981 tgtcaaagca tcaacagtga tcaaatcgtc gtcttcatcg tccatgtcgc gaagttcgat
    35041 ctctgtttct tcgatatcca agacacgcat gtccagacct agtgattgca attctttcac
    35101 caatacgcgg aatgattccg gtacacctgg ttttggaatt ggttcaccct tgacgattgc
    35161 ttcgtatgtt ttcacacgtc cgactacgtc atctgatttg tatgtcaaga tttcttgtaa
    35221 agtataagcc gcaccgtaag cttccagtgc ccatacttcc atttcaccaa aacgttgtcc
    35281 accaaattga gctttacctc ccaatggttg ttgtgtaacc aatgagtaag gtccgattga
    35341 acgtgcatgc aatttgtcat cgaccatgtg agcaagtttg atcatgtaca tcacaccgac
    35401 agacacacgt ccatcaaatg gttcacctgt acgtccgtcg tacagaacag ttttcgcatc
    35461 gctggccata ccggcttcac gaactgtttc ccatacgtct tcatcacttg caccatcaaa
    35521 tactggcgtt gctacgtgga tacctaactg acgtgcagcc atacccaagt gtaattccaa
    35581 tacttgcccg atgttcatac gtgaaggcac ccctaaagga ttcagcatga tatcgattgg
    35641 tgttccatca ggtaggaatg gcatatcttc ttccggcata atacgggata caaccccttt
    35701 atttccgtga cgaccggcca ttttatcccc ttcatggatt ttacgttttt gaacgatata
    35761 aacacgaact aacatgttta cgcctggtga caattcatct ccagcttcac gagtaaagat
    35821 tttcacatcg tgaacgatac cgccgccgcc atgtggtaca cgtaatgatg tatcgcggac
    35881 ttcacgagct ttttcgccaa agatcgcatg caatagacgt tcttctgcag ataattctgt
    35941 tacccctttt ggcgtgactt tccctacaag caaatcgcca tcttggactt ctgcaccaat
    36001 acggatgata cccatttcgt ctaaatcttt taatgcgtct tccccgacat tagggatttc
    36061 gcgtgtgatt tcttcaggtc ctaattttgt atcgcgggct tctgattcat attcttcaat
    36121 atggatagaa gtgtatacgt catcttttac caaacgacgg ctcatgatga tcgcatcttc
    36181 atagttgtac ccttcccaag tcatgaaacc aactaaaacg ttttggccta gagccatttc
    36241 gccttgttcc atagatggtc cgtctgccag agtatcccct ttttcgactt tctcacccaa
    36301 atgaacgatt ggacgttggt tgtagcttgt tccggagttt gaacgacgga attttgttac
    36361 atcgtattta tctaatgcac cgttgtcgcg gcgtacgcgg atttcagaag catcaacgta
    36421 ttcaacgaca ccgtcatgtt tacataacaa agcagctcct gagtcatgcg ctgatttgta
    36481 ttccatacca gtaccaaccc atggtgcttg aggattgatc aacggcactg cttgacgctg
    36541 catgttggca cccatcaagg cacggttgga gtcatcgttt tccaagaaag gaatacatgc
    36601 tgtcgcaacg gcaactactt gtttaggtga tacgtccatg tagtctactt tatcaataga
    36661 tacttctaag ttttcacttt gagcacgagc cataacaacg tcttgagcaa atgttccatc
    36721 ttcattcaat gggctgtttg cttgggctac gatgtaatgg tcttcgatat cagctgtcaa
    36781 gtagtcgatt tggtcagtaa cacgacctgt ttcacggtct acacgacgat aaggtgtttc
    36841 gataaatccg aatttattta ctttcgcata actggacaaa ctattgatca acccgatgtt
    36901 tgggccttca ggagtttcga tcggacacat acggccatag tgggagtagt gaacgtcacg
    36961 aacttcataa ccggcgcggt cacgagtcaa tccgccaggt cctaaggctg aaagacgacg
    37021 tttgtgtgtc aattcgccaa gagggtttgt ttgatccatg aactgtgaca actgagaaga
    37081 accaaagaat tctttgatac ttgccacaac tggacggata ttgatcaatt gttgaggtgt
    37141 caatgtttca gtatcttgga tcgacattct ttcacgaacc acacgttcca tacgtgctag
    37201 cccgatacgg aattggtttt gtaaaagttc accgactgaa cggatacgac gatttcctaa
    37261 gtggtcgata tcatcgacat tgccgatacc ttccatcaag ttcaagaaat agctcattga
    37321 agcaatgatg tcggctggac gaaccgtttt tacagctgct tctggatagc cgtttccgat
    37381 cacgtttact tcacgttcag ggtctcttgg tgaaaatact ttgatgactt ggacagtcat
    37441 tggatctgtg accacaccgt cttctgatgg atagtaagtc actgaattta ggccattatc
    37501 aatgaaagga gctagtgttt ccatgacttg gtgagtcaga actgttcctt tttcaacgat
    37561 gatctcgcct gtttctggat caactaatgt ttcagctaat gtcagattca atagacgtgt
    37621 tttcaaatct aattttttgt ttactttgta acgaccaacg tttgctaagt cgtaacgttt
    37681 tggatcgaag aaacgagcat tcagcaagtt acgtgagctg tctgctgttt ttggttcgcc
    37741 tggacgcaaa cgttcgtaaa cgtctttcaa accttcttct gttcttgagt cgcttgcgtt
    37801 tttatgcaag tctttttcga ctgtattgcg taatgtttcg ctgtcgccga agatttcaaa
    37861 gatcgtatca tcggaaccaa atcctaacgc acgaactaat acggttaaag gaatcttacg
    37921 tgtacggtcg atacgtacat aagagatgtc ttttgcatca gtttccattt ctagccaagc
    37981 tccacggtta gggataacag tagaaccgaa tccttctttt ccgtttttgt cgacttttcc
    38041 atggaaatag acacctggtg aacggactaa ctgagaaacg attactcgct ctgccccgtt
    38101 gatgatgaat gttccttgtt ctgtcattaa tgggaaatca ccgaagaata cttcttgtgc
    38161 tttgatttcg ccagtctcgc ggtttgtcaa acgcaatgtt acatgtaatg gtgcagagta
    38221 gtttgcatca tgcgcacgag cttctgctac tgtatacttt ggttctttca attcataatc
    38281 tacaaattcc aaagatagat tgccattgaa gtcatcgatt ggcaaaatgt cttcaaacat
    38341 ttctctaagt ccctcatcta agaaccattg gtaagagtct gtttgaattt cgatcaaatt
    38401 tggtaattcc aacacttcac tgattcttgc gaaacttcta cgttcgcgat gttttccgta
    38461 ttttactacg tgtccagcca agctcttcac ccctctaaaa tgtcattcaa aaagactacc
    38521 cgatcaattg ttacaataat atttcaaata ttatttttag gtaacaacag acattttttg
    38581 cggcttttag gcaaaaaaaa accaaaaata gaacattctc ttgaaacctt acttcgtgag
    38641 cttctacttt tgtttcctta tattaaagcc gaaacggaca atatttcgtt tctgcctttt
    38701 cttcatcaga tagtttttgc aacttcatat actacactta ttttctataa aagtcaacac
    38761 ttcctggcgt ttcttctgct ttttcgcaac attttcttaa tgttttcaat tttatgtttt
    38821 caattatctg acaattctta attgacagac ctttttatct cggttatact ttagaaaatt
    38881 cattttgtgg aggaacttct atgcgaacca acatcaacca aatcgcacca acacaactga
    38941 ataactttta ctttagctac tttagatagg tttgtattca tcatgatgga tacaaacgtt
    39001 aaacgacagt ttgtatctat gatggctaaa gaactttgta ttgcagcaaa aagccacgta
    39061 gacaaaatgc taagtggctg agcagtgaaa atacaaagta aaagtatcaa gcattctact
    39121 ttttcaattt attttccgac ttttcaatcc atttggacat tttgtctgaa tggatttttt
    39181 gtttcccaaa attaaggagg aaaaagaaat gaaaaaaaat ataatcatcg gattaggcgc
    39241 tgccctgcta ctagctttag gaggttgcgc acaaaaagac aattccgcag aggcatcaga
    39301 tggacaaaaa acaattggcg tattgcaact tgtacaacat aactcgttag atgctgccta
    39361 taaaggattc aaagaaggat tagcagaaga agggtataaa gaaggcgata atctaacaat
    39421 cgattatcaa aatgcccaaa acaatcaaga caacctgaaa agtatgagtg agaaactcgt
    39481 caaagaagat cctgatttgc tacttggaat cgccactcca gcagcacaat ctttattaaa
    39541 tgaaacaacc actatcccga tgactgtcac agcggtcaca gacttggaac aagcccattt
    39601 agtcacatcc aatgaaaaac ctggcggcaa tgtgactggc acgacagata tggtgccaat
    39661 cgaaaaacaa atcggcttac tgttaaatat cgtgccagac gccaaaacaa tcggcatcat
    39721 gtataacaac ggtgaagcaa actctaagat ccaagctgat ttggcagaaa aagaattgaa
    39781 aaaagcgggc gtcaaagtca aagtactgac agcaaattcc acaaatgatg tccaacaagt
    39841 gacgaaaagt ctagctaaag atgtagatgg aatctatatt cctactgata acacgttcgc
    39901 aagtgcggca agtgttgtcg gtgaagtcgc aaaagaaaca aaaacacctg tcgttgctgg
    39961 cgcagttgat caagtcaaag acggcggtct agcaacagta ggaatcgact acgaaagctt
    40021 gggcaaacaa acagggatca tggcagcaaa aatcttagac ggcaagaatc caagtgaact
    40081 tcctgtcgaa acagcgaatg atttggaact gtatgtcaat aaagacatgg ccaaagcatt
    40141 aggcatcaat ccagacagca tcaaagaagc tgaataaaac acaggaggat tctcatgaca
    40201 aatttgctct ctcttttctt atcatctgca tcgcaagggg tcttatgggc cctacttgcg
    40261 attggtgtat acgtcacttt ccgtatcttg gacattgccg acttgactgc tgaaggaagc
    40321 tttcctttag gtgcagggat cgcggcagta agtattacaa atggctatca tccattgaca
    40381 gcttgtcttc taggcttttt agggggtgcg gcagcagggc ttgtttccgg actgcttcac
    40441 acaaaattga aaattcccgc ccttcttgca gggatcatca ccatgactgg tttatattct
    40501 gtcacatccc gtgtgatggg tgcgccgaac atctctttat tgggtcagcc aacgatcttt
    40561 cattgggtcg agtcattcgg cataacaaaa gacaacgctg tactgatcgt cggtctgctt
    40621 tttgctttga tcgttgtatg tttacttgtt ttgttcttac gtacagaaac aggacttgct
    40681 attcgtgcaa ctggcgataa tctggcaatg agcgaagcaa acgggatcaa cacagataat
    40741 atgaagatca tcggttatat gatttctaat ggatgcattg ctctttctgg ctccttactt
    40801 gcgcaaaaca acggctttgc tgatttaaat tctggtattg gaacgatcgt aattggactt
    40861 gcttctatta tcatcgcaga agttttgtta cgtaaccagc cactatttct ccgtctcttg
    40921 actgtcgttt taggtgcggt catttatcgt tttattttag cgttagtctt cgaactgaat
    40981 gttgaaccaa gtgattcgaa acttgcttct gctttcgtgc tagtgatctg tctttctttc
    41041 ccgcagatcc agcaaaaatt ccatttacct caaaaaaaga aaggcggtag tgtcaaatga
    41101 atgacgtctt aacattggaa aatctccatc atacctttga aaaaggaacg atcaacgaaa
    41161 atcatgtcct gaaaggcatt aatctttcct tagatcccgg cgacttcgtg acgatcatcg
    41221 gtggaaatgg ggctggtaaa tctactttgc tgaacagcat cgccggtaca ttttctgtcg
    41281 atcagggtca aattttattg aatggaaaaa atatcacgaa aaaatctgtt gtcgaacgtt
    41341 cgaaatccat cagtcgcgta ttccaagatc ctaaactagg gacagccgtt cgtttaacgg
    41401 tagaagaaaa tcttgctctt gctatgaaac gaggaaaaaa acgcggcttc ttccgtggtg
    41461 tcaaaccaca agaccgaaca tttttcaaag accagctggt tcggctaaat ttggggttgg
    41521 aaaatcgttt atcctcagaa atcggtttgc tgtctggagg acaacgtcaa gctattacgt
    41581 tattgatggc cacattacag cgtccagaac tgatcttatt agatgaacac actgcagcgc
    41641 ttgaccctgc tacttccaaa acggtgatgg aattgacaga aaagctgatc actgagcaga
    41701 atctcactgc ttttatggtc actcatgaca tggaagacgc gatccgctat gggagtcgac
    41761 tgatcatgct gcatcaagga aggatcgtcg tagatatcca gcaagaggaa aaacagcagc
    41821 tcacagtgcc ccaattatta gcgttgttca aacaaaacag cggcacggca ttaaaagacg
    41881 atcaagtact tttatcttca taatcagaaa gaggatggga caaaaaatga acgcccaccc
    41941 tcttcttttt tctctttcct tctttcgttt ttgctttatg tatcaaccgt tgtttcttcg
    42001 aaaactattt tgttctctcc ttcttcctga tagaatggat gggaaaggag cttgatgcca
    42061 tgacaacgaa agacaaggta cttcaattat tgaaaaagac accagatttt ctttccggag
    42121 aaaagttggc acagcaactg gaagtttccc ggacaagtat ttggaaagcc atcaaagaat
    42181 tggaaaaggc aggctatcgt tttgagcacg ggcctacagg gtatcgttac ctgccctctg
    42241 acgtactaga tgcaaaagaa atccagcaag agctacggtc gtttattcct gatttgaaag
    42301 tacaaacgat gttgacttct gaatctacga tgaaagatgc caaactggca gcagctcaag
    42361 gagattctgt ttcgaagtta tttgttgctg atacacaaga acaagctcgt ggacgttttg
    42421 gacggccttt tttcgctgaa cctggacgag gaatctacat gagtctcttg ctccatccag
    42481 acaaacggtt tgatgaattg cctcaatata cgatcatcgc agccagtgcc tgtgtgaaag
    42541 ccattgaatc tgtaaccggt cgttctgctt cgatcaaatg ggtcaatgat atctacatgg
    42601 atggaaaaaa agtctgtggg attttatctg aagcagtcag tgatatggaa agcggcagga
    42661 tcagtcatat catcattggg atcgggctta atttttctgt tcctcaaaat cattttccag
    42721 ctgatctcca agaaaaagcc atctctgtct ttcccgacgg acaagctagt gtcacaagga
    42781 atcagctaat cagcaaaatc tggcaagaat tgttcactta tctttctcac ccagaaaaag
    42841 cgttggactt ctataaagaa aaatcattcg tattgggaaa aaccgtgtcg ttcaatcaaa
    42901 aaggaacgac ttatgtcggt cttgcaaaag aaatcggtga tcatggcgaa ctgatcgtcc
    42961 aaacaaatga acagaagatg agtttgtctt ctggcgaaat cagtctcaca tctatccgta
    43021 cgcaataatt atcagatttc acaaaaggac acctgatctc atacagtgtc cttttttctg
    43081 aattctcttc aaaagactct ccttattcta tcagctgaca gtttctctat cctctagaaa
    43141 aaggtattct aaaaatatct ataaagcatg gtacactttc tttaaatgaa tcaatgcaaa
    43201 aaacaagaga gtcatcagtt tacacgatca aaagagatag caggccgctt cctttttcaa
    43261 tcttatagat agaccatggg taacgataaa aacaaaaaag aacttgcgac ttattttgcc
    43321 gtaagttctt ttttagctag tgccttaatc tcttaatccc aacaaacgtc tcgcatagat
    43381 cacctgacca gagtgcatag cagcatcatc aataatagaa acgagacgca ttcctcgtgt
    43441 gactggtggt gtccagttct cgtcaattac ttcatctagc tcggaaggtt ccagccttct
    43501 taagtaagaa atagcaaaat cagcagctcc tcgcaagtat cctagcagat cttctatttg
    43561 attagcttgg atctgctttg cttgttccag actatgcttc caatcttgtt cgttatctgc
    43621 tatcgtcatc ggaaaccggc tttcccagtc ttgactatgc caaattggag aagttcctgc
    43681 taaatcagca atctggaaat ctaattctcg agcggtatgc caagccagcc aagtcattga
    43741 tttgatctgc ggggctctat cttccactgg aaaacgattg gcctcctcaa tagagacgcc
    43801 ttcaaacaaa cgttcaaacc gatcgtatgc ccgatccaca ctctcgcata gcatatctaa
    43861 ataatccatt tattttcctc ctttttattt atcctactta ctttttccta attctaggat
    43921 acctacttat atgttactac acacccttat tttttacact ttaacagtca ccatcatcaa
    43981 tagtttggag attgaaagaa aaagatagtg tcgaactatc aattgaacaa gaagcaacaa
    44041 tatgaacgtt tcattaatat tctttaaata aacaatgatt tttcaaacga acagtttaag
    44101 actaggagaa aaagagtata ataggaaaat gtttgaaagg aaaggaaggg gaaaaatgaa
    44161 aaaaagggca agtttactag tcctagttgc tttgttgttc aatatctttt cgtatggtgt
    44221 ggccgtttca gcggaaacat acattcctaa agaccctggg aatatttata atgatctaac
    44281 tggtaactat ggcttgttag gaattgctag tcaattccac gtatttgcta aaaataagac
    44341 aactgtgaat gcacatacag acggaaatat agcaacacat gaacttgatg caaaaaataa
    44401 ttttggtaca gagcttcatg aaggtttatt gaaccaagaa ataaattata tacaaaaaac
    44461 tgattctttc gctaattctt caggcatacc tacaggtagc acaagaatga ataaatttgt
    44521 cgttggcagt aacgtggatg tagcacttca agatggtcat cctacagtca atggatccag
    44581 gcttgatcac ttaactccag atgattttta taaagatcgt gctgggcatg aatatattga
    44641 tttcgagcag gaatttgcta aattgaatgt agcagcaaat gatcttgcaa cgatcactcc
    44701 agctaaaaca tatactgctg cagattttcc agacatgaac aatcggacaa tcgatttaac
    44761 tgatttatca gataccgggt ttttattggt aaatatcgat gcagaagtgt taacgatgaa
    44821 tacgccactt caaatcatta atccaaatga tcaagtagtt gtatttaatg tcatcaacag
    44881 ttcttctgca ttgaacgtac agtcaccaat caaatacaat gatcgttcaa atcatgaaac
    44941 agaagatttt tcggatgcga atatctcttg gaattttgga aatgagatga ctgatttgac
    45001 aatcagtgct ccttttcaag gaacaatcct tgcgcctaat gctactatcc gcattactca
    45061 aaaccaagac ggaacgatta taggaacaaa tgtgatttta gatgcagcta ccaatcgttg
    45121 ggatcctaac gaaatattta tcagcgacaa tggtacagat acgacagaca caagcgacag
    45181 tacagataca tctaccacaa gtgatagctc agattcatca acttcaagcg acagtacaga
    45241 tactacctct accacaagtg atagcacgga tacatcagct tcaagcgaca gtacagatac
    45301 tacctctacc acaagtgata gcacggacac atcagcttca agtgacagta cagatactac
    45361 ctctaccaca agtgacagca cggacacgtc agcttcaagc gacagtacag atactacctc
    45421 taccacaagt gatagcacgg acacatcagc ttcaagtgac agtacagata ctacctctac
    45481 cacaagtgat agcacggaca cgtcagcttc aagcgacagt acggatacat ctaccacaac
    45541 aagtgatagc acggacacgt cagcttcaag tgacagtaca gatactacct ctaccacaag
    45601 tgatagctca gattcatcta ccactacaag tgatagcacg gacacgtcag cttcaagtga
    45661 cagtacagat acatcctcta ccacaagtga tagctcagat tcatcaactt caagcgatag
    45721 tacagacacg tcgtctacca caagcgatac acgtcatgca agtgtctcct tctcaaaaac
    45781 aagttcggat aacaggacga actttaactt aactaataaa acaagcaatg acaatggaaa
    45841 caaatcaaaa agagcacttc ctaaaactgg aagtcaatcg aataattgga taacactcgc
    45901 aggcgttata ttacttgtaa tcggtttaag aatcactttt atgagttata agaaatcgaa
    45961 aaggtaaaaa aatattcaag ataaaaaaga gcaacgtcgc taacggacgt tgctctttat
    46021 ttttgctttt tatcataaat acaagaacta caattcaatt tgtcaaaaaa cacgtagatt
    46081 tccttctgag aaatttattt acgtatcgaa cctttcccta ttcagtcttt gcgatccaaa
    46141 atcagttggg taagggaaaa gagattctct tttgttcgaa gagaggtggc aggtaatttc
    46201 tttatttcct tcaaagcctt ttctgtgtaa gaggccgcta acttcctagc ttcttccacg
    46261 cctcctgtat gttcaacgat tttgcgtacg gcgtcgacat cttcttctgt cattgccgct
    46321 ttcttctcta aataaggtag tagctttggt ttgttttttt gcaaagagta gatcaacggt
    46381 aaagaataga ccccttgacg catgtcttct agtacaggtt tgccgatttc ctggctctct
    46441 tttgtataat ctaaaatatc atcgataatc tgaaaggcca tcccgataga aagtccgata
    46501 tctcctgctt tttttgcgaa gcgctcgctt gttccacttt caaaagcgcc aacagaacaa
    46561 cttaattgga acagttcagc tgtcttacct gaaatatttt ccaaatattc ttttacagaa
    46621 agttcataat tatagcgatc atccatctgc cctaattcgc cattcaatac tttttccata
    46681 ctgcgagaat tcaattggag acttttcaag gaagtggagt aatcagacaa tagtttaaaa
    46741 cagcagacaa ataaatagtc tcctgcatag acagaaacga cattaccaaa ctgactacgg
    46801 atattaggct gaccacgtcg cagatccgct tcatcaacga tatcatcatg gatcagtgtg
    46861 gccgtatgga gcatttcgat agaagctgcc aaagcgatgg ctttgtctcg atcttgctct
    46921 ggtccaaatt gtgagaacaa tagctgatag gcaggacgca acagctttcc gccggcatgg
    46981 atcgtttctt tgatggcttg ttcgacaggt ttgtttttca ggcggacagt ttcttccatc
    47041 cgttttagtg tttctgtcaa ttctttactt aaagcaggat agttttccca cattggatgt
    47101 agcttcataa aaaaactcct tgtgtgacga ttattttttg gtaaacattg ggctccattt
    47161 ttgtttggtt ccctgctcat aatctttttg gaatcgttga agtgtctgca catgtcgtaa
    47221 aagataattc gctgcgatcc cgattgcaat ccctgataaa atccctaaaa ctgaaaggac
    47281 tggcaagtac aacatgactg tccatgattg tgcaatgaaa gaagccatca aaagctgtcc
    47341 gacattatgc atgaaaccgc cggccgcact aattccgatg atactgacac gttttggtcc
    47401 taattgcttg accaataaca ttccaaaata actaagcaaa gcaccactca tactataaag
    47461 gaatgtggaa atcgttccgc ctagcaaagt agtcaggaat agtcgtagcc aaagtagtaa
    47521 gaagctgtct tgttttttca tggtaaaaat ggcaacgatc gtgattagat tggctaagcc
    47581 tagttttgca ccaggcgcaa aagaaaaagg ataagggatc atattttcca ataaaccaat
    47641 gatcactcct tgtgcaacaa gtagcgaaat ataaatcatt ttttgtaatt tactcatggt
    47701 gtgtgaaggt ctaataaatc agactgtctt cgtcacctcc atctgttgaa cggacttcga
    47761 tcactaattt atgtggcaaa cagacgatgg tttcgccatt tttcgttgcc cagcctcgac
    47821 gtacgcagat ctggtcatcg caatctgctt cttttatccg tactcgatcg ccatctatct
    47881 cgatcaagtt ataatctccg tgttcatctt tgtaagtata tgtgttgctc tcttctccag
    47941 ccatcaaagg gaatgttttg atttctgtgc catccacacg cagtaccgct tctttttctg
    48001 ctgttgcggt gttttgctga agaccaaaaa tcatgactgg taaaaaagaa agaacagtca
    48061 aaagcaaaat aataaaaaca tcccaaggtt tgagcagact tttttcagca aattctttaa
    48121 ggctcatttt tgttctcctt tttccagatt aactcaccca attttagcat aaaaacaaga
    48181 ggttgtgaca cctgtgtctt gctttgagta ataagaacga agaatagaaa acagctttcc
    48241 atgttttcta attctttggg cttattaccg agaagcagca ggtggaacac cgtttataac
    48301 aagaggttgt gacagaagtg ggcaacttca agaaataaga aggaatttac gaaaattact
    48361 tttcaagttt ttgtgaaatt tcggcttatt tttcgaagaa gctacttctg gaacaccgtt
    48421 tataacaaga ggttgtaaca gaggttgtga cacctgtgtc ttgctcatgg aggcttatct
    48481 cgggcatctt attcctttaa ttttacataa aaaggaggct gtgacggttt gtctcagccc
    48541 ctttctattg aaatcttgca ttcaaataac gttctttatc tatttatttc ttgccgtact
    48601 gggaacttgg tctaccgtac caccaattac ctagtgtacg ttgtagccat ggaattaccc
    48661 agcgatccaa cccaatcgca cgtccggaac cgttcatcaa tgcgaaagca acaaaa
//
LOCUS       DAZULO010000012        43773 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000012 DAZULO010000000
VERSION     DAZULO010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.012"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(413..2794)
                     /locus_tag="ACG8OA_000695"
     CDS             complement(413..2794)
                     /locus_tag="ACG8OA_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS4975534.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2784..3431)
                     /gene="recU"
                     /locus_tag="ACG8OA_000696"
     CDS             complement(2784..3431)
                     /gene="recU"
                     /locus_tag="ACG8OA_000696"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFS4975535.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3495..4034
                     /locus_tag="ACG8OA_000697"
     CDS             3495..4034
                     /locus_tag="ACG8OA_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFS4975536.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4124..4543
                     /gene="gpsB"
                     /locus_tag="ACG8OA_000698"
     CDS             4124..4543
                     /gene="gpsB"
                     /locus_tag="ACG8OA_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFS4975537.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4565..4932
                     /gene="rnpB"
                     /locus_tag="ACG8OA_000699"
     ncRNA           4565..4932
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8OA_000699"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5141..6307
                     /locus_tag="ACG8OA_000700"
     CDS             5141..6307
                     /locus_tag="ACG8OA_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFS4975538.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6353..7849
                     /locus_tag="ACG8OA_000701"
     CDS             6353..7849
                     /locus_tag="ACG8OA_000701"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFS4975539.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7930..8613
                     /locus_tag="ACG8OA_000702"
     CDS             7930..8613
                     /locus_tag="ACG8OA_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4975540.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9258..10448
                     /locus_tag="ACG8OA_000703"
     CDS             9258..10448
                     /locus_tag="ACG8OA_000703"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFS4975541.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10550..11503
                     /locus_tag="ACG8OA_000704"
     CDS             10550..11503
                     /locus_tag="ACG8OA_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFS4975542.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11572..12921
                     /locus_tag="ACG8OA_000705"
     CDS             11572..12921
                     /locus_tag="ACG8OA_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4975543.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13104..13427)
                     /locus_tag="ACG8OA_000706"
     CDS             complement(13104..13427)
                     /locus_tag="ACG8OA_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975544.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13556..14719
                     /locus_tag="ACG8OA_000707"
     CDS             13556..14719
                     /locus_tag="ACG8OA_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS4975545.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14789..16138
                     /locus_tag="ACG8OA_000708"
     CDS             14789..16138
                     /locus_tag="ACG8OA_000708"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4975546.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16183..16614)
                     /locus_tag="ACG8OA_000709"
     CDS             complement(16183..16614)
                     /locus_tag="ACG8OA_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4975547.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            17002..19644
                     /gene="alaS"
                     /locus_tag="ACG8OA_000710"
     CDS             17002..19644
                     /gene="alaS"
                     /locus_tag="ACG8OA_000710"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFS4975548.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19844..20440
                     /locus_tag="ACG8OA_000711"
     CDS             19844..20440
                     /locus_tag="ACG8OA_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4975549.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20562..21710
                     /locus_tag="ACG8OA_000712"
     CDS             20562..21710
                     /locus_tag="ACG8OA_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS4975550.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21748..22161)
                     /locus_tag="ACG8OA_000713"
     CDS             complement(21748..22161)
                     /locus_tag="ACG8OA_000713"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFS4975551.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22240..22671
                     /locus_tag="ACG8OA_000714"
     CDS             22240..22671
                     /locus_tag="ACG8OA_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFS4975552.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22770..23003)
                     /locus_tag="ACG8OA_000715"
     CDS             complement(22770..23003)
                     /locus_tag="ACG8OA_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4975553.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23186..24856
                     /locus_tag="ACG8OA_000716"
     CDS             23186..24856
                     /locus_tag="ACG8OA_000716"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFS4975554.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            25003..25389
                     /locus_tag="ACG8OA_000717"
     CDS             25003..25389
                     /locus_tag="ACG8OA_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975555.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25376..26074
                     /locus_tag="ACG8OA_000718"
     CDS             25376..26074
                     /locus_tag="ACG8OA_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFS4975556.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            26079..26543
                     /gene="lspA"
                     /locus_tag="ACG8OA_000719"
     CDS             26079..26543
                     /gene="lspA"
                     /locus_tag="ACG8OA_000719"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFS4975557.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26540..27451
                     /locus_tag="ACG8OA_000720"
     CDS             26540..27451
                     /locus_tag="ACG8OA_000720"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS4975558.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27686..28225
                     /gene="pyrR"
                     /locus_tag="ACG8OA_000721"
     CDS             27686..28225
                     /gene="pyrR"
                     /locus_tag="ACG8OA_000721"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFS4975559.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28239..29522
                     /locus_tag="ACG8OA_000722"
     CDS             28239..29522
                     /locus_tag="ACG8OA_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFS4975560.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29535..30461
                     /locus_tag="ACG8OA_000723"
     CDS             29535..30461
                     /locus_tag="ACG8OA_000723"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFS4975561.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30480..31760
                     /locus_tag="ACG8OA_000724"
     CDS             30480..31760
                     /locus_tag="ACG8OA_000724"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFS4975562.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31765..32841
                     /locus_tag="ACG8OA_000725"
     CDS             31765..32841
                     /locus_tag="ACG8OA_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFS4975563.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32846..36031
                     /gene="carB"
                     /locus_tag="ACG8OA_000726"
     CDS             32846..36031
                     /gene="carB"
                     /locus_tag="ACG8OA_000726"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFS4975564.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            36085..36861
                     /locus_tag="ACG8OA_000727"
     CDS             36085..36861
                     /locus_tag="ACG8OA_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFS4975565.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36858..37790
                     /locus_tag="ACG8OA_000728"
     CDS             36858..37790
                     /locus_tag="ACG8OA_000728"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFS4975566.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37787..38503
                     /gene="pyrF"
                     /locus_tag="ACG8OA_000729"
     CDS             37787..38503
                     /gene="pyrF"
                     /locus_tag="ACG8OA_000729"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFS4975567.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38510..39136
                     /gene="pyrE"
                     /locus_tag="ACG8OA_000730"
                     /pseudo
     CDS             38510..39136
                     /gene="pyrE"
                     /locus_tag="ACG8OA_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            39163..39441
                     /locus_tag="ACG8OA_000731"
     CDS             39163..39441
                     /locus_tag="ACG8OA_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975568.1"
                     /translation="MRFVSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFI
                     FLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            39479..40120
                     /locus_tag="ACG8OA_000732"
     CDS             39479..40120
                     /locus_tag="ACG8OA_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFS4975569.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            40163..40855
                     /locus_tag="ACG8OA_000733"
     CDS             40163..40855
                     /locus_tag="ACG8OA_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFS4975570.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40852..41754
                     /locus_tag="ACG8OA_000734"
     CDS             40852..41754
                     /locus_tag="ACG8OA_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4975571.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41912..43618)
                     /gene="efbA"
                     /locus_tag="ACG8OA_000735"
     CDS             complement(41912..43618)
                     /gene="efbA"
                     /locus_tag="ACG8OA_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFS4975572.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        14035 a         7707 c         9504 g        12527 t
ORIGIN      
        1 gaataacgag gtattgtttt cgataaaaaa agatggtgca gacaaattat gataattaag
       61 catactatca gataactcaa aatgaagcct tctttcaaaa aattattcaa aaccatatat
      121 tgtatttttt atttgataat aacaatggga ttattaagaa gtggcactaa ataaaagact
      181 agtgtgtatt tagccgattt aattataaac aagtaaatgg ataaagtata tgtaatgata
      241 aaagaaaaat ggattttcta atttttaagc taaatgttag atcacacatg tatatgctgg
      301 atagtcttat aaaaaatcat gtaatacagc ttggtacatg atttttattt gtagctgttt
      361 tttacataga cacaaaaaag aaaccacgct tgtagacgtg gtttcttttt tactatctgt
      421 ttccagaagc tgaggtgctg ctggatggtg tatggtcatt tgcagcatcc gctccgctag
      481 ttgctggctc actggaagaa gcaggaggag cagatgaaga cggtgtcgag ctttcttcgg
      541 atgaagaaga aggaaccgtt gttgaagacg ctggctcgct tgtttctgct gtcgtgcttt
      601 cttttgagga ctcagcagtt gattcgcttt gactagtaga gctagagctt tgagtcgttg
      661 tctctgttgt tgagcttcca ggagtctgga catagctgga agatggaata gttgttgatg
      721 gtgtaattgc attcgatcta gctgtatatt gatctttgta gtacagttca ccgcccacac
      781 gaattaatcc gcttggcatc tcccagtctg tatttgtcac gttagctgaa acatactgca
      841 taagctcgcg ataaacatca gaggctacgt gtgacgattc tgaagtgaca ggtgtcattt
      901 ttttattgta acctgtccac acggaaatcg aataatttgg cgtatagccc gcaaatagga
      961 tatctggata caccccagaa gaaataccaa gttttgcata ctcgtcatct gtgtaattgg
     1021 atgttccggt cttaccggct tgatataacc cagcgatttg cgcattcgta ccagttcctt
     1081 cagtgatcgt atcttttaaa atgtctgtga tcatataagc tgtttctgga gacatggctg
     1141 tttttccatc tggctcataa gtttcttctg ttccgtcttg gaagacgatt ttattaacat
     1201 attgaggttt gtaataagtc cctccattgg cgaaagccgc ataggctgct gccattttta
     1261 aggaagaagc tccgtatttt gtcccatctt gttcttccgt attactagag atcgcattcg
     1321 attgatggat cgtactgtat tcgatcccaa gattttttaa gaaagaagcc actttatctg
     1381 aaccgacttc attgaataat ttgactgctg gaacattacg ggataagtaa agtgcttggc
     1441 gtaaagtaat ggtgcccata tattgattat cccagtttcc tacaggagtc gaagtccctt
     1501 catagttata cggtgcgtca gtaatagttt ttccagtaga atatttcaaa tattcaaaag
     1561 ctggtccgta atctgtgaca ggtttcatcg tagaaccaaa atcacgggaa gtattcactg
     1621 ctaagttgtt gcctaaagtt acgtcttctg cgatgtgacg tccgccaatc tgggctttta
     1681 ctttcccggt attggtgtcg ataagggtgg aggcaacttg catctcgtcg tctgggtaag
     1741 agacatactg atccgtgttg acgatatcat atagtttctt ttgggcgtcc agatccaaat
     1801 tcgtataaat ctctaaaccg tctgtgtaga cattcttatc tgttttttct tgtacttcat
     1861 tgatgacttc tttgacataa ttatcgacga tcttagtatt gtcatcggat tgagttagtt
     1921 cttgcagacc atccgttacc ggtacattaa cagcttgatc atattctgtt tgagagattt
     1981 tttcgttttg aagcatcgta tacaaaacag tatctcgtct tttcttcgcc tgatctggat
     2041 aaacataagg atcataggcg gaaggggctt gaggcatccc agcaagcaat gctgtttgcg
     2101 gtaaagaaag ttctgataat ttcttaccaa agtacatctc agaagctgtc tccatcccgt
     2161 acaagccgtt tgacatatac actttattta cgtagtaggt caagatctct tgtttcgatt
     2221 ttttttgttc caatcttaca gccatccacg cttcttgggc cttgcgtttc agagtctgat
     2281 cctcggcact ggtggagaag aaggaaagtt tgatcaattg ctgtgtcaaa gtacttccgc
     2341 cttgcagtcc gccagaggta aagttagaga gggcagaacc gatgatccga atagggtcaa
     2401 ccccgatgtg tttataaaaa cggcgatctt ctaccgacac gatcgcatct tctaatgttt
     2461 tcggtaattc attcgcagaa attttttcac gcttttctgc gcctaaatct tcgaatagtt
     2521 cgccatcttg tgtatatagt ttggaagata cggtagcgtc taatttttta tccgtcaatt
     2581 cgggcgcatc ttttgcgtaa taccagaaaa gtcctacacc tgctaaaaag aggatacaga
     2641 aaaatgagag tattcctaaa aggatcttga taaataatcc ttttttatgt gttccagagg
     2701 actttccaga acctttgcca gaattcttct tcacactctt tttaggagta ggctgtttct
     2761 gtcgtctaga gcttctagtt tgttcatttg ccattcttgc tttctccttc aatagataat
     2821 gttgataaat agaagtcaac cgcgtccaaa taaggaatcc gcggagaaat accagttttg
     2881 atctctattc ctagttcttc gatcaaagat aaaggtagtg atttgcgtcc tttttccttt
     2941 tgggcgtccc agtaagcaat caaatgaaca ctattcaaat aaaaacagcg cttcaaagac
     3001 gaaaaccata acaagacaaa gcagacccca ttttgttcga ggcatgcttt catatgttcg
     3061 atttgatggg catggaagtt tttaaaggga aaagatgttt tgttcttggt ttcttttgct
     3121 tcaaagtcca agtaaaagcc tcgatagacg ccattatagt ctgtagttga ggcttgggta
     3181 aagtaggctt ccttgatcac agcagcactt ctcttaggat agtctacttt cacgatttgg
     3241 ataggggtag gctttttatg gataaccgcc aacccgcggg aaagataata tttattactt
     3301 tcattgattg cttcttcaaa gcgcatcccc cgattgccga attcagtgat ctgatttctt
     3361 ttttcttgaa ttttttcgac gggagaggca ttcttataat aaggttggcc atttggatag
     3421 cgcaatacca tctttaatca cactcctagg ccacattata gcaaaaaagc ggatagaaag
     3481 aaaaggaatc tgagatggat aagtcaaaag taatctatgt gtctgggtat cgaagtttcg
     3541 aactcggtgt ttttaaagaa aatgatccta aaatccaagt tgttaaaaat gttttaaaaa
     3601 aagaactcca tcagttagca gaagaagggc tggaatgggt acttgtcagt gggaatttgg
     3661 gcgtcgaact atgggctgtg caaatagtag cggaattgaa agaagaatat ccggaactat
     3721 ctcttggtat tttgtatcct ttttctgatt ttggtaataa ctggaatgaa gggaaccaag
     3781 aaaaaaaaca attagcagaa caattagcag attatgtcga agctgttagc catcaaccct
     3841 accaatcacc tagtcagcta aagaatcaca ctcgttttat acttgagcat acagtgggct
     3901 gtctgctgat ctatgatgaa gaattccctg gaaaaacgaa gtattttttt gaagatgcac
     3961 agaattacca agcagatcat ccctacgaaa ttcggctgat cagtatggac gatcttcaaa
     4021 attttagtga ataatcgata ttgtgtttga tttatcaact tttttcctat acaatgaaaa
     4081 aggtagaaat gttattacgt aaatgaaaac tagaggtgta aatatggcga atttggttta
     4141 tagtcctaaa gacattttac aacaagaatt caaaacaaaa atgcgcggct atgatcccgt
     4201 tgaagttgat gaattcttag acaatatcat caaagattat gaagcttaca gcaaagaact
     4261 ccttgcttta caagaagaaa acgatcgatt gagtgcaaaa gtagctcaat tatctaaaac
     4321 tcaaggagca gctcaaacac gcgtgcaaca aacagaggca ccaaagagcg cagcagtgac
     4381 caattttgat atcttaaaac gcttgtcaaa tcttgaacgt gaagtatttg gtaaaaagct
     4441 tgatcaacaa gcaagtgcag tcaaaccagc acaacctaat ccaaataact acacaaatgc
     4501 agacacaagc ttagatgata atgaaaaaac acgtcaattt taatttttga ttaatgaatt
     4561 tgcggttttc gggtaatcgc ggtctgactt gatcaggctg aggaaagtcc atgctcgcac
     4621 aggctgtgat gcctgtagtg ttcgtgctta gcgaaaccat aagctaaggt actctttgga
     4681 gtaacggcag gaaaacaagc taaggcttcg gctatgctta agtatccttg aaagtgccac
     4741 agtgacgaag cggctgggga aacccatccg gtggaacgcg gtaaacccct cgagcgagca
     4801 acccaaacaa tggtaggggc gctttttgtc tggaagaaga acaggacaaa aaggcagtat
     4861 aactgcagat agatggttac cgctttgttt tttctccctg gaaaagcaaa gtacagaaca
     4921 tggcttatag aaaaccgcaa acatcaggga attaggtcgt gacaaaagta atgcgttata
     4981 agaaacaaga aaaacatttg aaagctattt ttcaatactt cagatgaatc gttcttatta
     5041 caaaacaaac ttttgtatca ccggttattc ggaatttgag taaaagtgtg gggtaggact
     5101 gatgtcccac ctcctttttt tataaataag gagattgtgt atggaaaaaa acaggaagtt
     5161 caacctcgtc gcaactgcgg caagcgggct agaagcactg gttggtaaag aattaagaga
     5221 tcttggaatc gaatgccaag ttgagaacgg acgtgcacgt tttgaaggaa caatggaaac
     5281 gatcgctaca gctaatttat ggttacgtac agcagatcga gtaaaaatcg tggttgggga
     5341 attcgatgcg tatacgtttg atgaattgtt tgaaaacgta aaagcattgc cttgggaaga
     5401 ttttcttcca ctagatgcag cttttcctgt agctggaaaa tcgatcaagt cgaaactata
     5461 tagcacacct gattgccaag cgatcacaaa aaaagcaatc gttgaacgtc tccgtaccta
     5521 ttatcaccgt ccagcttctg taccactgac ggaaactggc gcacattttc agttggaagt
     5581 agctttgctg aaagaccatg tgatggtaac tctggatacg acaggaccaa gtttattcaa
     5641 acgtggttat cgcttagaaa aaggtggagc accattgaaa gaaaacatgg cagcagcact
     5701 tattatgttg acaaattggc gaaaagaccg tccattttat gatcctgtat gtggttcagg
     5761 tacgctttgt attgaagcag ccttaatagg ccataatatc gcaccaggat tcaatcgtga
     5821 gtttgcttgc gaatcttggg attggttcag tcaagaaatc atggaaaatg ttcgatcagc
     5881 agccgaagaa aaagcagatt atgatattga attagatatt actggttcag atatcaatgg
     5941 acggatgatt gaaattgcca aagccaatgc agaagaaatc ggattaggct cttcgattac
     6001 ctttaagcaa caagcagtca aagacttcaa aacggataaa gaatacgggg tcatcgtggc
     6061 taatccgcct tacggagaac gtctaggaga agaagaaact gttcggaaat tatataaaga
     6121 aatgggcgat gtattccgtc cattaaaaac atggagtaaa tatatcttga ctagcgactt
     6181 agcgtttgag gaattttacg gacaaaaagc aaccaaaaaa agaaaattgt acaatggtgc
     6241 attgcgtacg gatctattcc agtattgggg aactcgtccg cctagaaaaa aagagaaaga
     6301 cgaataatct tactaattga ttcattcaat tggatgtttg gaggaatagg gaatgaaaga
     6361 aaaagagttt ttacaagaag taaaagaaat caatcttcta caacaagctt taggaatcct
     6421 cgattgggat acacaaacag gtatgcctga aaaagctagt gcataccgaa gtgaagtaga
     6481 cagctatctt tacagcattt atttttcaaa aaaaatcggt ccaaaaatca aagaagccat
     6541 tcgctatttt tctgaacatc cggaagaatt gtcggaagtt ggaactgccg tttttgccaa
     6601 agtaaaagaa gagtatgatt tggaacatcg cgtaccggaa gatcttatga gaaaatatac
     6661 agctgccgtt tcttcagcac acggacaatg gcaaaaagct agagaaacac aacaattttc
     6721 tgattttcaa cacgcattga atcaaaatat tgaattaaca aaacagttga taccttactg
     6781 gagaaaagat gaaaagacga attatgatgt tttgctgaat caatacgaac ccgggatgac
     6841 tgtcgaaatc cttgataagg tatttgatca gcttaaagaa ggaattcttt cgatccgccg
     6901 tacgctccaa gaaaaaggga ctgaacctga aacagacttt ttaaatcgga gaatgacgaa
     6961 aaaacaacag aagagatttg tagtgaaagt aatcgaacaa cttggttatg attttagtcg
     7021 tggtcgttta gatgacactg tccacccatt tatgactgga attaatccga atgatgcacg
     7081 aatcacgaca cgatggaacg aaacagattt caaaatggct gtttttggag tgatccacga
     7141 ggctggtcat ggtatgtatg aacaagacat cgatgataaa tatgcgtata caccggttta
     7201 tgaaggtaca tcaatgggta tccatgaatc acaatcgttg tttaacgaga tcattattgg
     7261 aagtagtcgc agcttctgga aaaagcaata tcctttcttt caagaatgtg cagaaggaac
     7321 attcgatgat attccttttg acaaatttta tcgttcattg aaccatacaa aatcaagttt
     7381 ggttcgtatc gaggcagaca gtttgactta ccctctccat atcatcattc gttatgaaat
     7441 cgaaaaaatg attttcaatg aaggggtaga tgttgctgaa ctacctgaaa tatggaatca
     7501 aaaatatgaa gagtatctgg gaatacgacc aacaaatgat gtggaaggca tcctacaaga
     7561 cgttcattgg tcaggtgcaa gctttggtta ttttccctct tatgcattag ggtacatgta
     7621 tgcggcacaa ttacttcatt ctttgcagca agaaatagat gtggaaaatg ttctagcgtc
     7681 agacgattat tctccaatca aaaactggat gtcagagcat atccatcaat atggtgcatc
     7741 tagaaaacca aatcaattaa tcatggatgc aacacatgaa ccgcttaatc cgcaatacct
     7801 gatcgactat atgaaagacc tgtattttaa tgtatatcag gtaaaataaa atacaattta
     7861 caaagatagc tgaaattcat ctaatctata gatgttttca gctatttact taagaaagaa
     7921 gtggaacgaa tggataaatt acaagtgatc gctgattata gtatggacca attagcgcat
     7981 gatcaaacag gacatgggag cgatcatacc aaaagagttg tcaagttagc tgaaaaaatt
     8041 ttggagacgg aaccgcaggc agaccgcttt gtgacgcttg cagcagctta cctacatgat
     8101 acgattgatg ataaagtcgt taaaaatgag aacgaggcaa agcaacagct tcgtgtattc
     8161 cttcgctctt tgccaatagt agaggaacag atctcgatga ttttttccat tatcgaaaat
     8221 atgtcctttt ctaaaaatct ctctgaagcg gccgaactat ctctggaagg taagatcgtt
     8281 caagacgctg atcgtatcga agcattagga gcaatcggca ttttgcgtac tgcttatttc
     8341 ggcggtggac atgggcaccc gatttttgat cctgaactgt atccgcaaac attcaaggac
     8401 aaagaaaatt accgaaaagg cacgacagtg atcaatcatt tttatgaaaa actgttcttg
     8461 ctgtctgata aaatgaatac tgattacgga tatgaagaag ccaaacgaag agaaaccttc
     8521 atgcgtgaat tcttagaaga attttttctg gaatggtcta ttgacgatga aaattttact
     8581 ccagacaatt ggatcaaaaa taacaaaaaa tgatggaggt tgtgacagaa gtggtcagct
     8641 ccaagatatt tcggatcatt ttccgtaaaa gttacttctg gaacactgtt taccaagagg
     8701 tcgtgagcct gccgttcagc tccgagtcac aaggaacaat ttcaaaaaat agcttctcgt
     8761 gtttgatgaa tattgtaact tgtgattcgg aattggcagg agaacaccgt ttattcggaa
     8821 gaataggact gtgacaagac ttttgtcaca gtcctttttt cgaattcaac aagtaaaact
     8881 tttaagaaat attcagagag taaaattaaa aattgctgat aaacgaagat ggaaatttta
     8941 tgtattttta ttggtctatg caataaataa attaaatatt cagaatacaa ttgattttaa
     9001 cgaaagggct tctatcaatg gttgtgttgt atttatctgc ccaggtagag taaaatagat
     9061 cattatctaa aaggtaattg ttcatactat ataagtcatt tgaaagtaaa aagatactgt
     9121 aaataaaaaa tgtggttaaa taattctcat aatcagtgta agcgttatca aaaatatagc
     9181 atacttgctt tgtagatgaa tgacacgata tatgtttggt atgttctgca agacagatga
     9241 atatggaggt ggagacaatg tcttccaaag aactaacaat gtatttagag aaagaattgg
     9301 atcaactgaa agaaaagggg ttatacaata cgatcgacat actagaaagt gaaaatggtg
     9361 cttgtatcat cgtagacgga aaaaagatga tcaatctagc atccaacaat tatcttggat
     9421 ttgccaatcg cgaagaattg aaaaaagcat gtattgaagc aaccgaaact tatggtgtag
     9481 gagcaggagc agtccgaacg atcaatggtt cgctgaaaat ccatcagcaa ttagaagaaa
     9541 aaatcgcaga attcaaaggg acagaagcag caatcgcctt tcaatctgga ttcaactgca
     9601 atatgggcgc tatctctgca gtaatgacaa aagaagatgc tatcttatcg gatgaattaa
     9661 accatgcatc tattattgac ggttgccgct tatccggagc aaaaatcatc cgaattaaac
     9721 atcaagatat gaaagacctt gagaaaaaag caaaggaagc gatagaaagt aagaaatata
     9781 aaaaaatcat gtatatcaca gatggtgttt tctccatgga tggagacatt gcacgattgc
     9841 cggaaattat tccgattgtt gaaaaatatg gactgatcac ctacgtcgat gatgcgcacg
     9901 gctcaggcgt aactggaaaa ggtgcaggaa ccgtcaagca ttttggatta agcgacaaaa
     9961 tcgatatgca aatgggcacg ttatcgaaag ctataggtgt tgttgggggt tatgtagctg
    10021 gatcgaaaac actaatcgac tggttgaaag ctagaagtcg gccattccta ttttctactt
    10081 cgctaactcc aggtgcagct gcagctgcat tggcttcgat tactttgatg caagaacatc
    10141 cggaacttgt cgaaaaagtt tgggaaaatg ctaattattt taaagaagaa ttgaaaaaag
    10201 taggctataa catcggtatg tctgaaacac ctattactcc agtcattttg ggagacgaaa
    10261 aagtgactca gactttctct aagaagctga tcgaacatgg gatttatgct aaaccaatcg
    10321 tttatccaac tgtgcctctt gggacaggca ggatcagaaa tatgccgact gcggaacata
    10381 cgagggaaat gcttgatgaa gcagtagcag tttatcaaaa aataggaaaa gaaatggaaa
    10441 ttatttaact aaatttagtt ttttcgtcta tttaaaatta gagaacatgt tcacgcatat
    10501 attgttaatc atctgaagat agtagtaaaa aagatcaggg aggatcatca tgaaaaaagt
    10561 attggtgaca ggatgtttag gacaaattgg ttcagagttg atcacacggc tacgtcaaga
    10621 actaggcagt gaaaatgtca ttgcgacaga tatacgcatg cctgaagtca ttgagaaaaa
    10681 cgatatcttt gagatcctag atgtaatgga ttacgaaaaa atgcgcagca tagcagaaca
    10741 atatcaagtg gacacattga ttcatttggc tgctcttttg tcagctgttg cagaagcaaa
    10801 accacaatta gcctggaatt tgaacgtgac tgggctggtc aatgcattgg aagtcgctag
    10861 agaattccag ctgaaatttt ttacgccaag ttcaatcggc gcgtttggtc caaatactcc
    10921 aaaagatcat accccacaag atactcttca gcgtcctaca accatgtatg gggtaacgaa
    10981 ggtagctgga gaactactat gtgattatta ttatgtgaaa tatggcgtag atactcgtgg
    11041 tgtccgtttc cctggattga tttcttatgc aaccttgcca ggaggcggta cgacagacta
    11101 cgcagtcgat atttattatt cagcaatcaa agaaggaaag tacactagtt tcattgatca
    11161 aggtacatca atggatatga tgtatatgcc agatgcgatc gatgccatta taaaactcat
    11221 gaatgcagat cctgatcgat tggttcatcg aaatgccttc aatatatcgg cgatgtcttt
    11281 tgacccggaa gagatcaaag catctattca aaaaatcata ccagattttg aaatggatta
    11341 tgatgtggat cctgtacgtc agtcaatagc agaatcttgg ccaaattcac tagatgtttc
    11401 ttgtgcgcaa gaggaatggg gcttttcgcc gaaatacgac ttagatgtga tgacaaagga
    11461 tatgttggaa aaactgaaag aaaaattggt agagacggta taaaacaaga accacatcca
    11521 aaaagttttt tatacttttg ggattggcta tggtatgaaa ggggacaagg gatggaagaa
    11581 tatcaagcca cgccagtaaa agaagtaaaa atagaaaatg aattaaaacg tacgatggga
    11641 ttttttactg ctttatcgac tgttatgggc acagtgatcg gtgcaggggt attttttaaa
    11701 gccgcaagcg tagcagaagt tacaggatcc gccagtttgc atatgttttc ttggtttctc
    11761 ggtggtatga tttctgtctg tgcaggacta acaggagctg aactggcagc tgctattcca
    11821 gaaacaggag ggatgattaa atatatcgaa cggatttacg gaaatacagc agctttttta
    11881 ttgggctggg cgcaagtcgt gatttatttt ccagcaaatg tagcagcctt gtcgatcatt
    11941 ttcggtacac agttcgtcaa tctatttggt ctttcccagt caatgattgt tcccgtagct
    12001 gttacagctg ctgtatcgat cctgttgatc aattttttag gttctaaagc tggaggggct
    12061 tttcagtcga ttacgcttgt ctgcaagctt attcctttgt ttgtgatcgt cattttcggt
    12121 ttgttccgac aagaaggagt agacttccaa ctgtttccga tccaagcagg agaaaatctt
    12181 tcatttttct cagccttggg agcgggcttg ctagcaacga tgttcgcata cgacggttgg
    12241 atacacgtag gaaatatttc gggagaattg aaaaagccgg cgaaagattt gccaaaagca
    12301 atttctttag gaatcatcgg aatcatgatc gtttatttat tagtaaatgc ggtgttctta
    12361 agaaccgcat ctatcgacgg agtcgcagga aacagcaatg cggcaagtga cgttgcaaaa
    12421 atgatttttg gcggcttcgg tggaagactg gtcacagttg gtatcttgat ttctgtttat
    12481 ggaaccatca atggctatac ccttactgga atgcgtctcc cttatgttat ggcaaaagaa
    12541 aacaacctgc cttttagcaa gctctttgct aaacttcatg ataaaactaa agttcctgta
    12601 gccgcaggta tcttagagct agtcatcgct attggcatga tgatgatcgg tggttttgat
    12661 acactaacgg atatgctgat ctttgtcatt tggatttttt atacgatggt ctttgttggt
    12721 gtcattcttc ttcgtaaaaa agaacctgat ttgtttcgac catacaaagt tccaatgtat
    12781 ccgtttattc cactagtagc aatcattgga gggactttta tcgtatcaag cacgcttatc
    12841 actcagacct ttttagcttc gatggggata gtattgacac ttgctggtat cccgatttat
    12901 ctctatttaa aacgtagata gctaaaacat tcgaatttta ttagaactgc acttctctgc
    12961 caaacaaaat attttattta aatgaggagt acgtggaaga aatgtcaaaa gtactcgtta
    13021 attttgatca cgtaaatgtc gttttgtcta cgatgaaata gcaaagtaaa agacagaaat
    13081 cgggctcaac cgatttctgt cttttacttt gatttgcgta actgaactat tcggcgtacg
    13141 ccgcgataaa tatttaataa aaccaagcaa ccagaaatga aaatgggaag ccaaatcaga
    13201 tagacagggt agccaagcac tttagacaaa gcatgtggag tcattttatc catttcaaga
    13261 agtgcttgct gccaagtctg aagattgaag actagcatga aacccgaaat aatcaggaga
    13321 ataaatccta aataatgaat tgtagttctt tttcttacat aagcgatcgt agcataaagg
    13381 aacagtaaaa aaataggtac gccaaaaaca aagtacatat tcagcatctc gacaccccct
    13441 ttgtgtttat cttactttac tttgaaagaa atgagaaata atagcctcgt tacttgcttg
    13501 aaacagcaaa ggactgctaa gataagagag atacaaagaa tcaaggagga gtcatatggc
    13561 aagttatatc gaaccgattc gttttgcgct gattgttttt ccatttttag ccttagcgat
    13621 ctcatctgtt ttttttattt atgaatatcg aaagtatgga acatttctag taacaagggc
    13681 agttattttg tattctttcg ttttttattt attgtgtgcg tattttttgg tgatcttacc
    13741 acttccggca aaggagacgg ttgcccaaat gacagggcca aagatggagc tgcatttatt
    13801 tgcttcatgg caacatttca gagacaacac ggtattggtc ttgtctgaac ctagtaccta
    13861 cctgccggca atgaagcaga gtgtattttt ggaaccagct tttaatgttt tattactcat
    13921 accatttggc atttatttaa gatattattt ccggctttct tttatgaaaa cgatgattgc
    13981 tagtttctgc ctttctctat tttttgaatt gacgcagcta agtggacttt attttatcta
    14041 cccaagacca taccgcttat ttgatgtgaa tgatttagtg acaaatacat tgggtggaat
    14101 cattggtttt gtcgttacgc cgttatttac atttatgctt cctacaagaa gacgaatgga
    14161 tgaactttct tatgaaaaag gaaagactgt ctcattgact cgacgcttgg tcgcttattt
    14221 gatcgactgg acatttttat ctcttattaa tttggtagcg gcgattttct ttaaaattgc
    14281 gttcgattca ggggcagtta cttctcatat ctggtggctt ttattagaag taggggtata
    14341 ctttatgatt gtaccgttta taaccaatgg atggacactt ggcaaaaaaa ttgtgcgtat
    14401 ccgtttggtg gaagaagacc gcgaacgaat ttctttaaaa gcattgatga tccgctacgg
    14461 gtatctgtac tttctttttt atggtttaag ctggttgggg cagttgcttc agcaaatcat
    14521 gaattcgaca acgaatacgg tgttgcagat gagtgcagta ttcatttctc tcggtgtctt
    14581 gttactacaa ggtattttta ttttaaatat cttgctatca tttttgagaa aaaaacggat
    14641 tctgttttat gaaaaagcta gtcacacata tactgttagc acgattggcc aaacaaagga
    14701 tgatgattct cgaaattagc tgagcaaaaa cgctttgtct gtgttataat gccaaagtcg
    14761 tgataattta aagagaagag gtgcacgaat gaataatttt gatatattcc gcttcaaacc
    14821 gtttatcaat gaagcgttaa ccgcaaaggg cttcaaacag cccacagagg tccaggaacg
    14881 tttgattcct ttaattgcaa aaggaaaaaa tatcattggg caatctcaaa ctggtacagg
    14941 aaaaacacat acgtttttgt tgcctttgtt tgaaaaaatc aatgcagaaa aagaggaagt
    15001 acaagttgtt attacagctc caagtaggga actagctgca cagatctatg aagcagctac
    15061 acaaatcgct aaattttcta caccggagat ccgtgtctcg aattttgtcg gcggtacaga
    15121 caaacagcgt caattaaatc gtctgcatca ccaacagcca caggttgtag taggcacgcc
    15181 tggtagaata ttagacatga tgaatgaaca agcgctgaag attcacaccg catttgcatt
    15241 tgttgtcgat gaagcggata tgacattgga tatgggtttt ttaaatgaag tagaccaaat
    15301 tgcaagtcga ttaccagaaa agctgcagtt tttagttttt tctgccacta ttccagaaaa
    15361 acttcgtcca ttcttacgaa aatacatgga aaatccaatc attgaagaaa tcaaaccaaa
    15421 agcagttatt tctgaagaca ttgaaaattg gctgatttca acaaaaggta aagatgagaa
    15481 tcaaatgatt tatcaactgc tgacaattgg tcatccttat ttagcaatcg tatttgccaa
    15541 tacaaaacag catgtggatg aaattgccga ttacttgaaa aaccaaggct tgaaagtagc
    15601 aaagattcat ggagacatca ccccaagaga gagaaaacga gtgatgcgac aagtacagaa
    15661 tctggagtat caatatgttg tagcgactga tttagctgct agagggatcg atatcgaagg
    15721 tgtctctcat gttattaatg cagaaattcc agaagatcta gacttcttta tccatcgtgt
    15781 cggacgtact ggacgtaatg gcttaaaagg aacggccatt acgttatatt cgccaaatga
    15841 tgagcaagcc attgatgcga tagaaaaatt gggcgtctca tttgaaccaa aagaaatcaa
    15901 aaacggggaa atcgtcaaaa catacgatcg taatagacgt gccaaacgag aaaaatcaaa
    15961 agatgtatta gatcccacgc tgattggtct tgtgaagaag aaaaagaaaa agatcaagcc
    16021 tggttataag aaaaaaatcg attgggcaat tgcagaaaaa aataaaaaag aacgcaaaat
    16081 cgaacgcaga cagcaaagcc gtactgctcg taaaaataag aaaaattcac ataaataaaa
    16141 aacaagaggt tgtgacaaaa ggcgtgtcac aatctcttgt ttttaagcta gacttttttt
    16201 cattccgata caaagtactt catcttcaat aaaaggttct gtgcaagtat gatacccttg
    16261 tttttcataa aaatctacag ccgtcatttc agctactaca taactagtct gacaaccttc
    16321 tgaaaccgcc cgtttttcag cagcttcaag caactgaccg cctaaccctt ttcctcggta
    16381 ttcttttgct acgcaaaatc gttccggctt gaagcaagta ggtgttttct tttcataacg
    16441 caaagtagct actggtcttt tttgatcaag aaatagataa taggtttgtt gcgggatatc
    16501 ttttccgtcg aattctagtt ctgcaggaat cccttgttct aatacaaaaa cttgatatct
    16561 caaaaagcag ctagctgcgc gtatccattc atccgctcca actatttttt tcatcatatt
    16621 cctccttgtc atgaatagca gagatgtatt gattccttta ttcttatcgt accaaaaatc
    16681 gtggagtatg accaaaagga aatgaaaata aaaattgaga tattgacagt gatcaataga
    16741 atgcgtatac taattaagta cgaaaaggat atgatttgtt ttttaagact cacagaaaat
    16801 tctttcttgg ctgaaaaaag aatagtcact aatcaaattg ctcccttttt ctattttggt
    16861 agaaataccg acgcagctca gcgttatcga gtttaagagg taatgattac acatcattgc
    16921 aatcaaggtg gtaccgcgag ttttcgtcct tggctgtgat ggtgtttttt tgtatttaat
    16981 ttatagaaag caggtaatga catgaaacaa ttaagtagtg cacaagttcg acaaatgttt
    17041 cttgatttct tccaatctaa aggacatacg attgaaccta gtgcgtcttt gatccccgtg
    17101 aacgatccaa ccttattgtg gatcaactct ggtgttgcaa cattgaaaaa atattttgat
    17161 ggttcagttg tgccagaaaa tccaagaatc acaaatgccc aaaaatctat tcgaacaaat
    17221 gatatcgaaa atgtcggtaa aacagcacgg caccatacga tgtttgagat gctgggtaat
    17281 ttttcgattg gtgactattt caaaaatgaa gccatccact gggcatggga atttttgact
    17341 gatccaaaat ggatggcatt cgatccagaa aaactttatg taacagttta tccaaaagat
    17401 acagaagcaa aacgcatctg gcatgaagaa gttggcctgc cactagatca cattattgat
    17461 gtagaagaca acttctggga tatcggtgca ggcccatgtg gtccggatac ggaaattttc
    17521 tatgaccgtg gagaagctta taacgattta gcagaagacg atcctgaaaa ctatccagga
    17581 ggagaaaatg aacgttattt agaaatctgg aacttagtat tttcagaatt caaccataag
    17641 cctgataaca catttgaacc acttcctcat aaaaacattg atacggggat gggattggaa
    17701 cgcatggtct caatcgttca agatgcgtca acgaactttg agacagactt gtttatgcct
    17761 attatccacg ccgtagaaaa gctaagcaaa caagtaaaat atggtgaaaa cccagtaaca
    17821 gatatttctt acaaagtaat cgcagatcat attcgcgccc tttcatttgc tattggcgac
    17881 ggtgcattgc catcgaatga agggagaggg tatgtcttac gccgtttgct tcgtcgtgct
    17941 gtcatgcatg ggaaaaaatt aggtattgaa caagcattct tatatcgttt agtccctgtt
    18001 gttggggaaa tcatggtaag ttattatcct gaagtacttc aacaaaaaga atttattgaa
    18061 aaagttatcc gtacagaaga agaacgtttc aatgaaacaa tcaatgaagg tttggaaatc
    18121 ttaaatcaag tgatccaaga agtaaaaaac gaaaacggga cgactctagc aggaaaagac
    18181 atcttcaaac tatacgacac atatggcttc ccggtagaat taacggaaga ggtggcagaa
    18241 gatgaaggac tgaaagttga tcatgctggt tttgaaaaag aaatggatgc acaacgcgaa
    18301 cgtgctcgct ctgcccgcag tactgaaaaa tcaatggggg tacaatctgc tttattgaca
    18361 gatatcaaag tagaaagtaa attcattgga tacacagaac aagaatcaga tagtcaatta
    18421 ttggtcatcg ttaaaggtga gtctcttttg aatgaagtcg cagaaggtga agctcaaatg
    18481 atctttgatc aaacaccttt ctatgcagaa atgggtgggc aagtagcgga tcaaggatgg
    18541 atcatgaatg aagtaggcga aatcgtagca gttgttacag atgttcaaaa agcgcctaat
    18601 ggtcaattta tgcacaccgt agaagtaaaa gcaccaatga gtgaaggaca aacctatcat
    18661 ctattgatcg atcaatcgat gcgcaatcgg atcatcaaaa accatacagc aacccatcta
    18721 ctgcacaagg cattgaaaga agtacttggc gagcatgcaa atcaagctgg ttctttagtc
    18781 gctcctggac atttacgatt cgactttacc cactttggtc aaatcacttc agatgaatta
    18841 gctgaaatgg aaagaatcgt caacgaaaaa atctgggaag ctatcccagt tgaaacaatt
    18901 gaaacagata tcgatacagc caaaaacatg ggtgcaatgg ctttgtttgg tgaaaaatac
    18961 ggacatgacg ttcgagtagt aaatattggc ggctggtcaa tcgaactgtg cggaggcaca
    19021 catgtagcaa atacagaaaa catcggaatc ttcaaaatcg tttctgaatc aggaattggt
    19081 gcaggcgtgc gacgaatcga ggctgtaaca agtaaagaag cgtacgaact acttgaagaa
    19141 gaagaaaaac aactaaaaca agtagcaaca attgttaaat cacctcaatt gaaagaagtt
    19201 gtttcaaaag cggaacaatt gcaacagcaa gtaagagagc tacaaaagga aaatgaacaa
    19261 ttagctagca agttagcgaa tcaacaagct ggtgatgtat tcaaagatcc agaaacgatc
    19321 aatggaaaaa caatcattgc agcacaagta aaagtgaaag atatgaatca gctgcgtcaa
    19381 ttagctgacc aatggaaaca aaaaaatatt tcagatattc ttgttttagc aaacgaacaa
    19441 gaaggaaaag tcagtctgct agcggcaatg acgaaagaat caaatgaagc tggactaaaa
    19501 gctggcgact tgattaaagc aatcgcaccg aaagtaggcg gtggcggtgg tggacgtcca
    19561 gacatggcac aagctggcgg gaaaaatcct gctggtattc cagatgcact taaagctgcg
    19621 aaagaatggg ttgcgcaaca atagggcgtt tacgtttgac agaaaaacaa aagttcaaac
    19681 attgaacgaa acatgattat gggggcgaag acaaacaagt cttgccctca tacttttttc
    19741 aattggatgg cgcatctaat caaatggtcg gattcggatg gtctttagtt gacgaaagtg
    19801 acagaaattt ttacgattag tataaataaa aaggggtgaa aaaatgaata gtagaaataa
    19861 gacctttcga ctagtcttac gtgcgatctt gttagcaatc atcatcgtac aagcgatggt
    19921 tccttggctg gggtttattc cattaggatt cattagttta acaattatcc atatcacagt
    19981 aattattgct gcagtagtat taggcccaaa agatggcatg gtgattggct tgttttgggg
    20041 aatcgctaca attgttcgtg cctatgccat gccaacaacg ccatttgata cattagtgtt
    20101 cacgaatccg attatctcgg tagttcccag agtacttgtt ggcttagtag caggcttggt
    20161 tttccactgg atttatcaaa aaaataaatc aataacgatc agctcagcct ttgcaggggt
    20221 actgggttca ttggtcaata cagtcttagt gttaggattc atgggattat tttatacagg
    20281 tgcaacggca gatgcctatg gtgtagactc tgctctttta tttaaaacct tagcaggggt
    20341 tgcagcaatc aatgggatac cagaagcaat aggtgcggga atcataacac cattactagc
    20401 aaaagcgtta tttgcagcaa caccgttgaa accagaataa tgaccatctt ttaatagtaa
    20461 taggacgcac gatagataga aaaaggaaga gaggagagga aaacaatcgt cctctccatc
    20521 tttttacctg agcaacaagc aaactagaag gggtaaaaaa cttggctaag aaacaagaag
    20581 aaaaaaagaa acaaataccg caacagcgga caaaaaaagg agtactagcg ggtatcttag
    20641 gattggttac gaatctgatc ttgtttgtct ctaagctaat gatcgggctt ttttcaggaa
    20701 gtgtctccat tatggctgat gcagtgaaca gtctatcaga tactgcttca tccatattga
    20761 ctttgatcgg atttaaaatt gctgcaaagc cagcagatca ggaacatcct tatggacacg
    20821 aacgcttcga atatatcagt gggttatttg tttcgatcat cattacttat gtaggatttc
    20881 aatttttaga gtcttctgct gaaaaaatca tccatccaga aaatgtctct ttaacaccca
    20941 tcgtcttttt tgtcctaatc atttccatct ttttgaaatt tgcacaaggc aaaatgtatc
    21001 aggttattgc gcaatccatt gattctgaaa cattgagagc tacagcaaca gatagttata
    21061 atgatgtctt tactacactg gctgtattgc tttcagcagc aatcgaatgg gtaactggat
    21121 ggcgtattga tggatatata ggttttgctt tagcattata tatcctttat agtggattga
    21181 cgatgcttcg aaattttgta tacgaactat taggaagtcg cccaacgcca gcagagattg
    21241 aagaaatgga agagcattta aatgcttgcg atgatatact tggctatcat gatttattgg
    21301 tgcataacta tggacccaat aaacgctttg catcagtcca catcgaagta gatgaatcaa
    21361 tgaatttgaa tgtggctcat aagattattg atctgataga aaaagacttt aaaaagtctt
    21421 tggatgtgga acttgtctgt cacttggatc caatgcctgt tgataacgaa aaatactata
    21481 atatcttgcg gcagttaaag caaattcttc ttgaaacagg tacagggctt aatattcatg
    21541 attttagagt aattcatcaa ggaaaaacgc tgcaatttga tgtagtggtc cctgagaaat
    21601 tccagtttgt tgatacagaa ctagaaagaa tcttacgtag tcaaatagaa gaaaaaatcg
    21661 gagattacca attggaaatt acgtttgatc acaattatct tttatattaa atgagaaact
    21721 ataaaaagac tccttaaagt ttttaattta aagagtcttt ttttctttta attgtttatg
    21781 gagagcctga cgtgcaagat tatctgctcc tttatttttg ttttcgggca accattgcag
    21841 gatcaataaa gaaaattcgg atagaagttc ttggattctt tgaaggtagg gaaggaagag
    21901 ctcgttcttc gttgcgtttt tgtcgaccgt agaaactagt gttttgctgt ctgaaaaaca
    21961 gaaaatcgtt tcattctgta agtcgttttt ctttagaaat tctaaagttt ttaaaaagac
    22021 ttcgaactct gcttgatggt ttgtccctat agaaagcggg atagatagct gttcttgttt
    22081 accttgatag acaatcaaga ttccacctcc actaggacca gggttgcctt ttgtagaggc
    22141 atcaatgtat acttttaaca taaaaagaga ttctcctttt tgttcaaact aatgttatgg
    22201 taagattata tcattactga atcggtgaga ggttagagaa tgaaaaaaaa tattcgatgg
    22261 cagcctgaac tggcacatac gatcatctat tggtcattga ccttgatcat tctgtttttt
    22321 agtctgattc tttctttaga aaacacaggt ccttattgga aaagcaattt tgttttaggt
    22381 atttttcttg tttttctatt tctaggtttg cggcgttctt ttattttaaa atcaaactct
    22441 gtgattatac gttatgctgc tttttggaaa gaccgagaaa tatctgtcgg acagattcga
    22501 tctttgacgc caactaatac tggggtgcaa atgatgttgc aagatgagaa agccccatct
    22561 gttttcatga tgcgaaaaaa agatcaagta gtcctttttg atcattttga aaaaaacgga
    22621 cccaaatcag aatcaattcc ttatccttat acgaaaaaaa gaaaaagcta attttaaaaa
    22681 tcagaaccta aaaaaacgaa tttttacagg aaaagaaaag agtgagacgt acggttcatc
    22741 ccgtttagcg tctcactcta tttgtttatc tattcatgca tactagtatc ggtagtctga
    22801 acgttgcctt tcttgacatg ggcagcttgt gttcctcgat tcccttcgat gatctcaaat
    22861 tcgaccactt gattttcttc caaagttttg aatccctctt cttcaatcgc agtaaaatgt
    22921 acaaaaattt cttctgtgtc atcataactg ataaagccgt atcctttttt gttgtcaaac
    22981 cattttacaa ttcctgtggt caaagcactc acgacctttc taaagtctga tcatagcctt
    23041 cattataaag agtatttata agtacgtcaa ataaattaaa aacctgtaat tttatgtttt
    23101 ttttctgaat aaaaaaaggt ataattggca tatcttaaca aagaagatat tttgttgaat
    23161 aaagttcgtg aaaaggggta atcaaatgaa gaatgatctg caaatttcac aagaaacaga
    23221 gctagaaccg attgttgaag tggctagtaa attagatctt aaggaagacg atttggaact
    23281 gtatgggaaa tataaggcga aaatcaattt ttctggaatc aatcgatcaa aggataacgc
    23341 tgaggggcat ttgatcttag ttacttccat caatccaaca ccagctggcg aaggaaagtc
    23401 aacgatcact gtagggttag gagacgcgct caataaaatc aataaaaaat ctgttattgc
    23461 attgcgagaa ccctcacttg gtcctgtgat gggaatcaaa ggtggtgctg caggcggagg
    23521 atatgcacag gtattgccta tggaagatat caacttgcat tttactggtg acatgcatgc
    23581 tatcacaaca gctaataatg cattatctgc tcttttagat aatcatattc atcaaggaaa
    23641 tgaattgaac atcgatgccc gccgagtgat ttggaaacgg gtagtcgatc tcaatgatcg
    23701 tgaactgcgt aaagtaattg ttggcttggg tgggccaatc caaggtgttc caagagaaga
    23761 tgggtttgat atcacagttg ccagcgaaat catggctatc ttgtgtcttg cggcagacct
    23821 tgaagattta aaggctcgtc ttgcaaggat cgttgtcggc tatacatttg accgtcagcc
    23881 tgtgactgct ggagacttga aagcagaagg cgctctggca ttactgctga aagatgcaat
    23941 caagccgaat cttgtacaaa caatttacgg aacaccagct tttgtccacg ggggaccgtt
    24001 tgccaatatc gctcatggct gcaatagtat tttagctacc aagacagcat tgagattagc
    24061 agattacgtt gtcacagagg caggatttgg tgcagatctt ggcggagaga aatttttaga
    24121 tatcaaagta cctaacttga aaaaagcgcc ggatgctgtg gtgattgtcg ccactgtccg
    24181 tgcattgaaa atgcatggcg gaatgaaaaa agatgaactt aaaaatgaga atcttgatgc
    24241 tttgaaagta ggttttgcaa atttaaagcg ccatatacgt aatatggaac aatatcagct
    24301 acctgtgatc gtcgcaatca atgaattcgt aacagataca gacaatgaac tcacactgtt
    24361 ggagcacttg tgtgaaaatc aggggattct tgccaagaga gcgagtgtat gggcagatgg
    24421 agcagaaggc ggcgttgatt tagcaaaagc tgtagtacga ttgattgata gaaaagaagc
    24481 taattacaag ccattatacc gcctagaaga aacgatacaa gaaaagacag aaacaatcgt
    24541 caagaaaatt tacggaggaa atggtgttgt tttcagcaaa aaagcgcaaa aacaaataga
    24601 agagtttacg aagaatggat gggacaaact gccgatttgt atggcaaaaa cacagtattc
    24661 attttctgat gatccttcac tgctaggtgc accggaagat ttcaccatca cgatccgcga
    24721 aatcatccca aaattagggg caggattttt agtagccttg actggagatg ttatgacgat
    24781 gcctggtttg cctaaaaaac ctgctgcatt gaatatggac gtgacaaatg acggagaagt
    24841 gttgggattg ttttaagctt gattataatc taccatcgaa ggagctgtat tttgactaca
    24901 gcttcttttt ttagacgata gggaattaat tttgtttaca ctaggatcat tctttttctt
    24961 tattgtattt tgagctatac taaagcataa taggagggta tgatgaaaac gacatttata
    25021 agaaaaaccg gtttttacgg tatgggtagc tcgattcagc taagaatcaa caatgaaaaa
    25081 cgcagtgttc tcagtcatga acaaaagata acgcttgatc tggaagtgcc ctttacgatg
    25141 caggtcacct tttattggct aaaaagcccc gtttatacag tgaaagaagc aaagcctgct
    25201 tatgtgatca caatgaatta cttgattctt caaatctatc cgtttttatt tctagcaact
    25261 gggttggggg ccgttttcct acaaaattta tttttcagtt tttttgcagt tattgtgttg
    25321 attgcgttct ttttctatat aaaaaaccgg gcatatttga tcaaggagga aaattatggc
    25381 aaactctgat ctttttttaa tcagtttcaa tcgaatcgaa aaatggatga gagacgagat
    25441 gggaaatgca cgaaatatgg gatttaccga acttgtcaga cgcctagctc aaagaaaaca
    25501 attaatgatc agaaaatacg aagatgatct tttacagctt gctcagttaa ggaatgcgat
    25561 cgtccatgat cggatcgcag tcgattttgt cattgcagag ccaaatgatt ggacagtcaa
    25621 gcgaatccag ctgatcgaac aagaactgat tcagcctgaa accgtgctgc ctcgatttgc
    25681 aaaacgtgta acaggttttg aacaagatct tccgttactt gaactgttaa agatagtagc
    25741 agaaaaaagg tattcacaat ttccgttata caataaagga aaatttgtag cattgattac
    25801 tttacgtaat atcgggtttt ggctagcaaa agaaagccaa aaaggtccgg ttgatttaac
    25861 aaataaacga gcgttggatt tgatcatcca aaacggaaag tacacaaatt atcattttgt
    25921 tccagctaaa acacatattt acgaggttga agcgatgttt cgtgagcaag ggactttgga
    25981 agccgtactt atcactaaag atgggaatcc cgatggtaat ctgctcggga tcgtacgacc
    26041 tcgtgatatt tataaacaaa tagaaaagga ttgatttttt gttaattatt tattggatat
    26101 tcagcgcttt gctagtagga gtggatcagt tggtcaaatg gtggatcact gataatctag
    26161 cgcttggaga gacgaaaaat cttataccag gtattttgtc actgaatcat attcgaaaca
    26221 ctggagccgc atggagtatg ctagaaggga agatgtggtt ctttactata gtgacactca
    26281 ttgcagtcgt tgtgattctc acattgatga ttaaaaatcg tgaaaaaggt aatcgctggt
    26341 tcatgattgg attgagtttg attctagctg gtgcgattgg caattttatc gaccgtattc
    26401 gtcttggcta tgtagtagat atgttccaga cagattttat gagctttccg atttttaacg
    26461 tagcagatgt gacacttgta tgtggagtga tttgtatgtt aatttatatt attttagatg
    26521 aaaaggatca aagaaaaaaa tgacagaact taaaacgacg attcaccatg aatctggtag
    26581 gatcgataaa gtattgacta atttattcac cgattacagt cgttcgcaaa ttcagctgtg
    26641 gctgaaaaat ggagcggttt ctgtcaatgg acagccagtc aaagcaaatt acaaagtaaa
    26701 acaaggagac gagattactg tcttgatccc cgaaccagaa acattagatg tggtcccaga
    26761 aaatatcccg ttgacgatcg tttacgaaga tgaagatgta gcggtgataa acaaacctca
    26821 aggaatggtc gttcatcctt cagcaggtca tacgcatggc acaatggtca atgcgctgat
    26881 gtaccatatc aaggatcttt ctacgatcaa cggcgttatt cgtccaggta ttgttcaccg
    26941 aatcgacaaa gatacttccg gactgctaat ggttgccaaa aatgaccagg cacacgaatc
    27001 attagctcag caattaaaag ataagacctc tttgcgcaaa tatgttgctt tagtccatgg
    27061 agagattcct cacgaaaaag gaaccatcaa tgcgccaatc ggccgttcga aggaaaaccg
    27121 gaaaatgcag gcgataagag aagatgggaa gtcggctgtc acacatttta ctgtattgga
    27181 acgtttcgaa ggatttactc tggttgaatt aaaactagaa accggaagaa cacaccagat
    27241 ccgtgtgcat atgaagtata tcggttatcc gttagctgga gatccagtat acggaccgaa
    27301 gaaaactttg aaaggcaatg gtcagtttct ccatgcgcaa atgttaggtt ttacgcaccc
    27361 gactacaaac gaaaagatga tctttgaagc acctcttcca gaagtttttg aaaaaacttt
    27421 agaaaaatta agaaaaaaca ttgcatttta atttagataa cggtatagta attaacaacg
    27481 aaagcataaa acttaataga attcctttaa gtttagtccc gtgaggctaa gaagggtggg
    27541 agcaaggata cgtatggtgc gccataagta gattgctcat tgacatagta tagctatcag
    27601 tgtatcagta tacgctcagt ctaaacattc ctcctgtcct cttagggcag gagttttttt
    27661 atgcaaaaaa gtagggggaa tgaaaatgca agcaaaagaa gtagtagatc aggtaaccat
    27721 gaagcgagca ttgactcgaa ttacttatga gattatcgag agaaaccata gtatccaaga
    27781 tatcgtattg gtaggtatca aaacacgagg aatctacatt gcatcaagaa ttgccgaacg
    27841 cctgaagcag ctagaagata tcgatattcc tgtaggagaa ctggatatca ctttataccg
    27901 agatgacaaa aaagaaaatc cagaagaacc ggaacttcat tcttctgaca tcccagtttc
    27961 cttagaagga aaagaagtta ttttgattga tgatgttttg tatacaggac gaacgattcg
    28021 agcagcaatg gacgcagtaa tggattttgg cagaccaaga aaaatttcat tagcagtcct
    28081 ggtcgatcgt gggcatcgag aattgccaat ccgtgcagat tatgttggga agaacattcc
    28141 aacatctcgg gcggaagaga ttttagtaga gatgcaagag cttgatggac aagatcgcat
    28201 catgattttg aaagaggaag attagaggag agagaaacat gtcggagaaa aaaggatttc
    28261 ataatgatga cgtggtgtta gatatccacg atcgcccaac cccaggacat tgggtaggat
    28321 taagtttaca acacttgttc acgatgttcg gagcaaccgt gttagtcccc atcttagtag
    28381 gtatcgatcc aggtattgcg ctagtcagtt caggactagg tacattggtg tacttattta
    28441 ctactaaagg aaagatacct gcgtacttag gaagcagctt cgctttcatt gcagcgatgc
    28501 agcttttgat gaaaacagat ggttatccag cgattgcaca aggagcagtc acaactggat
    28561 tagtctattt gattgttgcg ctgatcatca aaaagatcgg ttcagcatgg ctggataaaa
    28621 tcttgcctcc aatcgtcgtc ggtccagtca tcatggttat cgggttggga cttgcggcaa
    28681 atgcagcaac aaatgctatg tataatcagg gcacgtatga tttcaagtat attgctgtag
    28741 cgctgatcac attaggatta acaattttct tcaacatgtg gctaagaggt tttctcggat
    28801 tgattccgat tttactaggt attattagcg gatacatcgt agccttgctg tttggaatcg
    28861 tggatactaa gccaatcgta gacgctgcct ggtttgctgt tcctaacttt gagattccct
    28921 ttgtacaata tgaaccaaag ctatatcttg gagcaatcac aacaatggca cccatcgctt
    28981 ttgtgacgat gacagagcat atcggccatc ttatggtatt gaataaacta actaaacgta
    29041 atttctttga agaaccgggt cttcataaga cgttgacagg tgatggatta gcacaaatcg
    29101 tggcagcctt tgtcggaggt cctcctgtaa caagttacgg tgaaaacatt ggtgttctgg
    29161 ctatcacacg tgtccatagc gtgtttgtga tcggtggagc tgcagtattt gcagtcattt
    29221 taggattcgt aggaaaatta agcgctttga ttttaagtat ccctggacca gtcatctcag
    29281 gaatcagttt tatcttattt ggagtaattg ccgcaagtgg aatgaagatt ttaatcgaga
    29341 acaaaatcga ttttgataaa aagaaaaatc tgttgattgc ttcagttatc ttagtagtag
    29401 gaattggcgg actcgtcttt gaagtaggca catttacatt gtcggcaatg gcattagcaa
    29461 ctgtacttgg catcatttta aatcttatct taccagaaac ttcacgaagc gaagaaaact
    29521 aaggaggaaa cattatgatc atcaaatcag aacgtatcag cttaaagcat ttattgaccg
    29581 ttgaggcact tactgatcaa gaagttatgg gcttgatccg cagagggcag gaattcaagc
    29641 aaggtgctaa ttggactccg caaaagcgcc agtactttgc tacaaacctc tttttcgaaa
    29701 atagtactcg cacacataaa agcttcgatg tagcggagaa aaaactaggg ctagaagtga
    29761 ttgaatttga agcaagcaca agctctgtcc aaaaagggga aacactatac gatacagtac
    29821 tgacgatgtc agcattaggc gtagacgtag cggttatccg tcatggtgat gagaactatt
    29881 atgacgaact gatacaaagt aaaacaatcc agtgttcgat tatcaatgga ggagacggaa
    29941 gcggacagca tccaacccaa tgcttgcttg atttgatgac catttatgaa gaatttggtc
    30001 attttgaagg tctgaaagtt gccatcgtag gtgatatcac tcactctcgc gtagctaaat
    30061 ccaatatgca gatgttaaaa cgtctaggcg cacaagtatt cttctcaggt cctagagaat
    30121 ggtacgacga tgaattcgaa gtgtacggac attatatgcc attggatgaa ctactagatc
    30181 aagttgatgt catgatgatg cttcgggtgc aacacgaacg ccatgatgga aaagaaagtt
    30241 tctcaaaaga aggctaccat caggaacatg ggttaacagt tgagcgtgcg aagaagttgc
    30301 aaaaacatgc aatcattatg cacccagcac cagttaaccg tgatgtagaa ttagctgatt
    30361 cattagtgga aggattacag tcgcggatcg ttcagcaaat gagcaatgga gtattcgtca
    30421 gaatggcaat cttagaagca gtgctaaatg gaaaagcgta attataggag gatgaaaaaa
    30481 tgaaaacact cattaaaaac ggtcagatca atacacgaaa aaatatgacg acaccagcag
    30541 aaatttggat agaagatggg cgaatcaaag caatcggtac aggattctca gaagccgaat
    30601 ttgatgaagt gtttgatgca aagggacaat tgatcacacc tggtttagtg gacgtccatg
    30661 ttcatttgag agagccagga tttacataca aggagacgat cgaagctggg actcgctcag
    30721 ctgctcgtgg cggtttcact actgtatgcg cgatgccgaa tttaaatcct gtacctgata
    30781 cggcagaaaa gctgagacaa gtatatgaca tcattagaaa agatgcagtt gtgaaggtat
    30841 tgcagtatgc gccaatcaca gaaaatctgc gaagtgagaa attagttgat caagaagcac
    30901 tgattgaaga aggtgctttt gctttcacaa atgacggtgt aggtgtccaa acagctggga
    30961 cgatgtatct ggctatgaaa gaagctgcaa aaaacaataa agccctagta gcccatacag
    31021 aagatgaatc tttattattt ggcggcgtta tgcacgctgg caagaaggca gaagaacttg
    31081 gtcttcccgg gatcttaagc gtaaccgagt cttctcaaat tgctagagat ctgttgctag
    31141 ctgaagcaac tggtgttcat taccatgttt gccatgtttc tactaaagaa agtgtccgtg
    31201 taatcagaga cgcaaaaaaa gcaggaatcc acgtaacagc agaagtttca ccccatcatc
    31261 ttattttgat cgacgaagat attcccgaag atttcggctt ttggaaaatg aacccgccgc
    31321 taagaggacg ggaggacaga gaagctttga tcgaaggctt gcttgacggt acgatcgatt
    31381 gcatcgctac agaccatgcc ccccatggat tagaagaaaa aagccaaagt ttcatgaaat
    31441 caccatttgg tatcgtagga agcgaaacag cgtttcaact gatctatact cattttgtag
    31501 aaactggtcg attcacgttg gaacaagtga tcaattggtt ggcagttaaa ccggctgaga
    31561 tttttggttt gaatgcagga acgctaacgg ttggtgcccc cgctgatgta gcagtttttg
    31621 atattacaca aacttgcacg atcgataaag aagacttttt atcaaaaggg gagaatacac
    31681 cttttatcgg ctggaaagta aaaggcgaaa cacaaatgac ttttgtcaac ggaaaacttg
    31741 tgtggcagaa gggagaataa aagtatggaa cgtttgctta tattagaaga tggaacagtt
    31801 ttcgaaggaa aggcttttgg tgcagatgct agtgtcttcg gagaaatcgt ttttacaaca
    31861 agtatgactg gttatcaaga aactatcact gatcaaagtt ttaacggtca aatcattaca
    31921 tttacctatc ctatggtggg taattatggg gtaaatagag atgattatga atcgatttct
    31981 ccaacatgca aaggagtagt agtaaaagaa catgcacgtg tagcttcaaa ctggcgaaac
    32041 caaatgacgc tagattcatt tttgaaacaa aaaggaattc ctggaatatc aggtatcgat
    32101 acgcgcgccc ttactcgcaa aatccgtgaa gtcggaacga tgaaagctag tatagttgat
    32161 gctggagatt ccttcgaaca tgcatttgat cagctgaaag caaccgttct agcaaccaat
    32221 caagtgcagc aagtctcaac taacaaacct tatccaagtc caggaacagg tcggaaagtc
    32281 attgtcattg acttcggctt gaaacatagt attttacgag aattgagtaa aagaaactgt
    32341 aacctcacag tacttcccta taacacagat gccgagacga ttttagcgct atcaccagac
    32401 ggagtcatgc tgacgaatgg acctggtgat ccaaaagatg tgccagaagc aatcgaaatg
    32461 atcaaaaaaa tccaaggaaa agtaccgatt tttggtattt gtctcggtca ccagttattt
    32521 gctttagcca acggtgcaga tacctataaa atgaaattcg gacatcgagg attaaatcac
    32581 ccggtacgag aagtagcgac cggaagaatt gattttactt ctcaaaatca tggctatgca
    32641 gtcgatgaag ccacgattga tccagaaaaa ctcatcgtaa ctcatgtaga agtaaatgac
    32701 ggaacagtag aaggagtcaa acacagagat tatcccgctt tcagtgtgca gtttcatcca
    32761 gatgctgccc ctggtccgca tgatgcacta catttatttg atgaatttat ggaaatgatg
    32821 gacgcaggaa aggagcagta aacagatgcc gaaacgtaca gatatcaaga aaattatggt
    32881 gattggatct ggtccgatca tcattggtca agccgcagaa tttgactacg caggaacaca
    32941 agcctgcctt gccttaaaag aagaaggata tgaagtggtt ttagtaaatt ctaatcctgc
    33001 gacgatcatg acggataaag aagtggcaga tcaagtatat atcgaaccga tcacacttga
    33061 atttgtatca aggattcttc gaaaagagcg tccagatgct ctccttccaa ctttaggcgg
    33121 acagacaggc ctcaatatgg caatggagct ggcagcatca ggtattttag aagaattgaa
    33181 catagaactt ctaggaacaa agctttctgc gatcgatcaa gcagaagatc gtgatctatt
    33241 caaacagttg atgaaagaat tgaatcagcc aatccccgag tctgaaatcg tcaatacagt
    33301 agaagaagct gtagattttg caaataaaat cggctatcct gtcatcgttc gtccagcctt
    33361 tactttaggc ggaactggtg gaggtatgtg tgaaaatgaa gaagaattac gtatcgtatc
    33421 agaaaatgga ttaagtcttt cccccgctac ccaatgtttg attgaacgaa gtattgcagg
    33481 ttttaaagaa attgaatatg aagtgatgag agattcggct gataatgcaa tcgttgtctg
    33541 caatatggaa aattttgacc cggtgggtat ccatacaggt gattctatcg tatttgcccc
    33601 tagtcaaaca ttgtctgacc atgaatatca aatgcttcgt gatgcttctc tttcgattat
    33661 tcgagcactt aagatcgagg gaggctgtaa tgtccaactc gctcttgatc cacatagttt
    33721 taattattat gtgattgaag tcaatccgcg tgtttctcgt tcatctgcgt tagcaagtaa
    33781 ggcgactggc tatccgatcg ctaagctagc tgctaaaata gcgcttggat taacattaga
    33841 cgaaatgaaa aatcctgtta caggaacgac ttttgcagaa tttgaacctg cattagatta
    33901 tgttgtagct aaaattcctc gctggccgtt tgataagttt gaaaacggag aacggctttt
    33961 aggcacgcag atgaaagcaa ctggtgaagt gatggctatc ggtcggaata tcgaagagtc
    34021 tttattgaaa gctgtgcgtt ctttagaaat cggtacacac catatcgaac ttcctgagtt
    34081 ggcagacatc acggacgatg acctaatcca aaaagtggtt cgagcacaag atgatcgcct
    34141 gttttatgta gctgaagcta ttagaagagg gtacaccata gaagaattag cggatttgac
    34201 aaaaatcgat ttattctttt tagataaact gttgcatatc gtagaaatcg aacagcagtt
    34261 gaaacagcag ccgttggatg acaagatatt gaaaacagcg aaacaaaacg gttttacaga
    34321 caaaaaagtg gcggagctgt gggaaataaa cgaagaagcc gttcgccaga tgagaaaaga
    34381 aaatgatttg attcctgtat ataagatggt tgacacttgt gcagcggaat tcgaatcaaa
    34441 aacaccttat ttttatagtt cctatgaata tgaaaatgaa agttcacgat cagaaaagcc
    34501 ctcaattcta gtactgggat ctggtcctat ccgtatcgga caaggggttg aatttgacta
    34561 tgcgactgtg cattctgtca aagcaatcca gcaggcagga tacgaagcaa tcatcatgaa
    34621 cagcaatcct gaaacagttt cgactgactt ttctatttcg gataaattat attttgagcc
    34681 attgaccttc gaagatgtga tgaatgtgat cgagctagaa cagccgatag gagtaatcgt
    34741 tcaatttggt ggtcagacag caatcaactt agccgaacct ttgactgctg ctggtgtaaa
    34801 aatcttaggg acaacgatcg aagacttgga tcgtgcggaa aatcgtgatt tattcgagca
    34861 agcgctgaaa gagttggata ttcctcaacc tttaggggat actgctacaa gtaaagaaga
    34921 agccgtggtt atcgcttcga gaattggtta tccagttctt gttcgtccaa gctatgtatt
    34981 aggcggacgt gcgatggaaa tcgttgaaaa ccagcgagat ttagaagatt acatggaaca
    35041 tgctgtgaaa gcatctccag aacatccagt actagtggac cgttacttgc ttggtggtga
    35101 atgcgaagtg gatgcgattt gtgacggtga aacagtactc atcccaggga tcatggagca
    35161 tattgagcgt gctggtgtcc attcagggga ttcaatggct gtgtatccac ctcaaacatt
    35221 atctgaatcg atcaagaaaa cgatcgaaga ttacacgatc cgcttagcat taggtttgaa
    35281 ttgtgtcggg atgatgaata tccagtttgt catccatgaa aatgaggttt atgtgatcga
    35341 agtcaatcct cgagcaagtc ggactgttcc attcttaagt aaggtgacag gaattccaat
    35401 ggcacaaatt gcgacgaaag ctattttggg agagaaacta aagacacttg gttatgaaaa
    35461 cggactttat ccagaaacaa aagaagttca tgttaaagca cctgtctttt cttttacgaa
    35521 attgcagaaa gttgatactt acttagggcc tgaaatgaaa tcgactggag aagtaatggg
    35581 atctgacaga caattagaca aagctttgta caaggcattc gaagcttctg gattacactt
    35641 gcctgaatat ggagccgtat tatttacgat tgcggacgaa acaaaagaag aagcactaga
    35701 attagctaaa cgttttacag aaattggcta tagtctgata ggaacgaaac atacagctgc
    35761 ttatttcgaa aaaaatggtc tggtcgttga aacagtagcg aaaatttctg aggaagcagc
    35821 agaaaaaaat gtagtcgact taataagaga tggtaaaaca caagtggttg tcaatacgat
    35881 tgataaagat cggggaaatg cttctaaaga cggtttcatc atccgaagag aagcagttga
    35941 acatggtatt ccattgttta cttctttaga cacagcagat gcgatcgttc gagtgatgga
    36001 atcccgtgct ttttcaacta accctattta acggcgagca catgttaaga caaaaagacg
    36061 aaaaaccgtt gcaggagggg acagatgaaa caagaaatga tggaaatcgt tcagcaaaaa
    36121 cagctggcac caaagatttt tgaaatgaca ttgaaaggtg atctagtaaa agaaatgcag
    36181 caaccaggac agttcttgca tattctcgtg ccgcgtgcag acttgctatt acgccgaccg
    36241 attagcttga atcatatcga ccacgaaaca aatacttgcc gtatcattta tcgggtggaa
    36301 ggtgagggga ctagagtctt ctctactctt tctgccggag accatttaga tgtcatgggt
    36361 cctttaggca atggttttga tttgtccaat cttcatacag gagacgaagt atttataatc
    36421 ggtggaggaa tcggtgtccc tcctctatat gagttatcaa aacagttagt cgaaaaagga
    36481 attaagccaa cgcatttttt aggttttgcc acacatgaag tcgtttatta tgaaaaagaa
    36541 tttaatcgtc taggagaaac aagaatcgct actgacgatg gaacatacgg tatgcacgga
    36601 aatgtcggta atttattgtt aggaacgaag acggaaccta cagctgtttt tgcatgtggt
    36661 tcaaacgggt tattgaagac agtggaacag ctgttttctt cgcatcaaaa tgtccagtta
    36721 tcattagaat cccgcatggc atgtgggata ggggcttgct atgcttgcgt ttgtcatacg
    36781 ccggaagatg aaacaggaac aaaaagtgtg aaagtttgtg acgaaggacc gatttttaaa
    36841 ataggagagg taattatatg aatgagaatc ctttagcggt caaacttcct ggcctagaat
    36901 taaaaaatcc tatcatgccg gcaagcggct gtttcggttt tggcaaagaa tacggaaaat
    36961 actatgattt gaatcagctt ggatcgatca tggtcaaggc aacgactcca aatgcccgct
    37021 ttggcaatcc cacgccaaga gtggcggaaa caccatctgg catgttgaat gcaattggtt
    37081 tgcaaaatcc tggtctagac gttgttatgc ataaacttct tccagaatta gaggagtaca
    37141 atgagttacc catcatcgca aatgttgctg gtgcttgtga agaagactat gtagaagttt
    37201 gtagcaaaat tggcgaagca ccaaatgtga aagcaatcga gctgaacatt tcttgcccaa
    37261 atgtcaaaca tggcggaatt gcttttggaa cagattctga tgttgctttt caattaacac
    37321 aagcagtaaa gaaagtttct tctgttcctg tctatgtcaa attgtcccca aatgtaacag
    37381 acatcgttcc aatcgctcag gcaatcgaag ctggaggggc agatgggttt acgatgatca
    37441 atactcttct tggtatgcgg attgacctta agacaagaaa accgatttta gccaatcaaa
    37501 caggtggact ctccggacca gcaatcaagc ctgtcgctat tcgtttgatt caccaagtag
    37561 caagtatatc cagccttcca atcattggga tgggcggcgt gcagacagtg gatgatgttc
    37621 ttgagatgtt tatggctgga gcaagtgctg tagctatagg gaccgcgaat ttcaccgatc
    37681 cgtatatctg tcctaaacta attaaagagc tccctgtgcg aatgagcgaa ctaggcatcg
    37741 aatcgctgga aagattaatc aaagaagtga gagaggaacg tgaacgatga atcaactaga
    37801 ccaacgacca attattgcat tagatttttc aactaggcaa gaggtagaag actttcttcg
    37861 atttttcccg aaagaagaaa aattgtttgt gaaaatcgga atggaattgt tctaccaaga
    37921 aggacctgaa atcgtacgtt atctaaaaga ggctggtcat aatgtatttt tagatctgaa
    37981 acttcacgat attccgaata cggtcgaaaa agcaatgcgg ggattggcaa agcttggtgt
    38041 tgatctcacc tgtgttcatg ccgctggcgg tattcgtatg atggaggcgg caatgcgagg
    38101 attagaagag gggactccag aaggtggaaa acgtccgcta cttctagcta tcactcagct
    38161 tacttctaca agtgaagaag aaatgcatgc cgatcaacta atcgaagttt ctttagaaaa
    38221 aagtgtgatc cattatgcta gctgtgccaa aaaagcagga ttagatggtg tggtttcttc
    38281 tgcttgggaa gtagaagcga tcaaagaaat ggcaggagac gaatttgttt gcttaactcc
    38341 gggtataaga ccagaaggaa cagtcgcagg agatcagaca agagtagtca caccttctca
    38401 ggcaaaaaag atcggctcaa cttttatcgt tataggtcga ccaatcactc agtcaactaa
    38461 tccttataaa gcatatcaaa cgattcaaac agaatggagc taacctaaaa tgaatgttga
    38521 acaaagtatc gcaaaagact tattagaaat tgaagcagta tttttaaatc caagtaatcc
    38581 ttttacatgg gcaagtggaa tcaaaagccc aatttactgt gataaccgaa tcacgatgag
    38641 ctatccaaaa gtccgaaaag agatcgcaaa aggattagca agcaaaatca aagaagctta
    38701 ccctgaagta caagtgattg ctggaactgc aacagcagga atccctcatg ccgcatgggt
    38761 agcggaaata ctggatttgc caatggtcta tattcgaagt aaggcgaaag atcatgggaa
    38821 aggtaaccaa atcgaaggac gtatatttga aggtcagaag atggttgtga tcgaggattt
    38881 gatttctaca ggcggaagtg tcttggaagc agctgaagca gcaaaaagag aaggtgcgga
    38941 tatccttggt gttgcggcta tctttacgta tgagttgcca aaaggaaaag caaatttcga
    39001 aaaagccgaa atccctctta tgacgttgac caattattct gtattgattg aagcagcttt
    39061 agaagatcgt tatatcgatg agcaagaatt aactctgcta aaagaatgga aaaaagatcc
    39121 tgaaaattgg cagattgaag cgcgcttgat gaagatagag aaatccgatt tgtgtctcca
    39181 gaatcatcaa ttggaatagc aaaaatccaa aaactgattg cgagaagcat cttttcatca
    39241 ggtatgaaaa gaaacttcta tcgtgatcag tttttgattt ttttaaaatt catttttcta
    39301 agactggtta gcagaaagaa gagaataatc tcttctcttt tttctattat atatagattt
    39361 tctctatttt ttggtaaggg agctgtttca aaaatggtaa agagcggaaa tgttgcgaat
    39421 atcagttatt taatgatatg atattcggaa aaagaaatct caaagggagg taacttttat
    39481 ggggttgaat gaatttattt taggattgaa caatttagag acgatcacaa gggcacctgg
    39541 attttttaaa tatacagagc atacagttgc agcacattcg tatcgtgtag catctattgc
    39601 gcaagttcta ggagacattg aagaagcgaa tggacaaccg atcgattgga aactgctcta
    39661 tgaaaaatca ttgaatcatg actacacaga gcgttttatt ggtgatatca aaacgccggt
    39721 gaaatacgca agcaaagagt taagagggat gctgcaaaca gttgaagaaa agatgacgga
    39781 cgaattcatc aaccaagaga ttccagctga gttccaagaa gtttatcgcc gtcggttgtt
    39841 cgaaggaaaa gatgaaaccg tagaaggaga actattagcc atttcagata aggttgacct
    39901 tctttatgaa tcttttgatg aaatcatcaa aaataacccg gaaaaagttt ataaggagat
    39961 gttcattgag gcagtgacta cgattaaaag ctttgaacat cggccttctg ttcagtattt
    40021 ctttaaagag atatttccgg aactattgaa ccaggaattt tatggaaaag aggcgttttt
    40081 agaaaccgtc tcaacgattg taaagaaaga agaaaaataa aaatgatatg agatgcaggg
    40141 ggaagtatag ttgaaaaaga atatggatgt cgaatggggc tattctggag acaaaggtcc
    40201 ggaaaattgg gcgagtttat gtgattggtt tgcgagaggt gcggaatttc cattgcagtc
    40261 gcctattcat ttgaccaagg gggaagaaac caaaatagaa ggagacacgc tttcctttca
    40321 ttatcaaaag caacggttta ctgacaagga attcaaaaac acgattcatt tagtaccttt
    40381 tgaccaactc agttatgtag agttcaagaa agaacgctat tatttgacag atattcattt
    40441 ccacttgcct tctgaacata tcatcaacgg aaaacaggaa gatattgaat ttcattttgt
    40501 tcatatgaac agcaaaaaag agaatctagt tgtgggtgtc atgtaccagc taatggtaca
    40561 ggaaggttgg ctgtataatc aagaaaatgg ggaaagttgg aacttagaaa aacatgagca
    40621 ttgggtaaat ccggatgtgt tctttccgga gcaaaaaagc catttccatt atattggctc
    40681 attgacaact ccgcctacca gcgggccaat ccaatggttt gtaatggatc atgtaggaaa
    40741 actgaaccaa gagtttcttc tccccttcga tggtcagtat gctcgaccga acaaccgacc
    40801 aatccagccc ttgaatggaa gagaaattta ttatttcgag gagtttgagc aatgaatatt
    40861 cagcagatga aatacgtggt cgctgtagca aacaatggta gtttcagaga agcggcaaag
    40921 aaaatgtttg tctcccagcc aagtctttca gctggtatca aagaattaga aaccgagtta
    40981 gggatcacgc tgtttactcg aacgaatcga ggggcatatt taacagaaga agggcaagac
    41041 ttcttaaatc gtgccgagaa aatattggtc cagcttgata cattagaaac tcactatcta
    41101 acgagtgaaa agagagacaa tttttcgatc gctgcccagc attatgattt cttaggtcct
    41161 ttgaccgcac agctactcga ggaattcaaa gacgagatca aacaatttcg gatcatggag
    41221 acgacaacag cgagagtgat cgaagaagtc aaagagcagc acagtgagat cggcattatt
    41281 tatatgaatg agcataatca agctggtatc agaagatatt tacaacaagg agagctaagt
    41341 ggccgcagca ttggaaaatt caaaacgcat atttttttaa ggcaaggaca tccattagct
    41401 gaacacgcag agatcaccaa agaagaattg ttatcttatc ctcaagttcg ttttacacag
    41461 gatggaaata atttcccgta tttttatgaa gaccttatcg agatccctga tcaagaaaca
    41521 gtgatttata cgagtgatcg aggtactttg atgaatatcg ttcttgaaac tgatgcttac
    41581 gcttcaggat ctggtattgt gattggaggg attcgcgagc acttgcggtt aatccctttg
    41641 gcagaaggcg aactaaatga attttatatc atttattcgg cgaaacgtcc gctcagtgaa
    41701 atcgctcaaa gatttatcaa agagcttact cggttactgg accagcagag ctaagaaaag
    41761 cagatgagaa cgctgtttat tgaacaccgt tgatcaaaaa agaggggttg tgacagaatt
    41821 ttgtcacaac tcctcttttt gtagcaaaca atttataact aattgctgtt gtgggaaagt
    41881 acgcaaatat gattctagaa acactcattg attacctgtt ttgtttcata gctgtaattt
    41941 tttcttcttc tggtgtcacg ataatcgtct tttggttttc ataaataaca taaccaggct
    42001 tggcgccatt tggttttcgg atatgcttta cctgtacaag atcaaccggc acttgggcag
    42061 agtatcggta ttttgaaaaa taggcagcta actcggcagc ttctgtaatt gtttcatctg
    42121 agggcttgtc acttttaatg atgacgtgcg aaccgggtat attttttgca tgaagccaat
    42181 agtcggtttt tttagctgtc ttcattgtca gctggtcatt ttgaagattg tttttaccaa
    42241 caagaatgag cgttccgtca gaagaaaagt attgatcagg ctgagatggt ttcttacgtt
    42301 tttgtttttt taagttcttc tttttcaggt atccttctga agtcaattct tctttaattg
    42361 cttcgatatc cattgggccg gcaatttcca gttgagataa gacagattca agatattgga
    42421 tttcatcttt ggcttcttgg atttgttcgc caatcagctt aacagcgttc ttcagctttt
    42481 ggtatcgatg gaaatatttc tgtgcatttt gatttggtgt caatgccgga tctagtgcta
    42541 ttttgattgg ccgatcttct tcataataat taggtaaaac aacttctttt gcgccgcgtg
    42601 gtacttgtgt cagaaaagta gtaagtagtt cgccatttct acggtaattc tcagcgtttt
    42661 cggattcttt gagtgtttgt tcacgttttt tcaatttatt tttatttcgt tttagttcat
    42721 tttcgatttt tcgaatcagt tctccgcctt gttgttttgc ccgatctttc tctgcttttt
    42781 cgtgataata gctatctaat aattgactga gtgttggata agctgttaca gcagatgcct
    42841 gttcggacaa tacctgataa gcaatcggtg tgaaaaaatc tttattttcc gtttcgtaaa
    42901 aagtaggaat aggctgatgg ctgatggctg agaaaaattc gtcccataca accatctttt
    42961 cattaggacg ttccagtaat cttttggtca gctcttgagc gatatcgaaa ccgattccct
    43021 gaaactgacg ttggattgct tttggatcta gttcagtttg ggaaagccgc tgaaagattt
    43081 tttctttctc ggagctaaat gggttcaaga cctcttgttt tggcggagca acatattcta
    43141 caccaggaag caaagaacga taagtgtttt gagagcttcc aatgtgtttg atcgcatcca
    43201 gtatttttcc agtttctcga ttaactaaga cgatcgtact atgacgtccc attaattcaa
    43261 cgatcaatat gatattttgt agatcaccca actcgtttcg tttagcaaag tggaaatgga
    43321 tcactcgatc attttcgatt tgttcgatgt cttctaaaat agcgccatcc aaatattttc
    43381 gaagcatcat cacgaagtta ggcggattat caggattctg ataatcgatt tgggtaatct
    43441 gtacacgagc ataacttggg tgcgcagata ataataatcg atggtttttg ccgcgtgaac
    43501 gaatcaccag cacgatttca ttttcataag gctgatggat tttcgagatc cggccagata
    43561 gtaacgtttc acgcaattcg ttgaccatcg cgtgggtaaa tacaccatca aaggacatgg
    43621 ttcattcctc atttcttttc ataagtatat tcattataac gaaaaaaaga agaaaaacca
    43681 atttggccga atagtaaagc aaaagtctag aaaaatcttg gactaattac ataaaaaaca
    43741 gtgtttctat cgttgaaatc caaattttta ggt
//
LOCUS       DAZULO010000013        41003 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000013 DAZULO010000000
VERSION     DAZULO010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.013"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..1189)
                     /locus_tag="ACG8OA_000736"
     CDS             complement(38..1189)
                     /locus_tag="ACG8OA_000736"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFS4975573.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="ACG8OA_000737"
     CDS             complement(1202..1639)
                     /locus_tag="ACG8OA_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4975574.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="ACG8OA_000738"
     CDS             complement(1654..3420)
                     /locus_tag="ACG8OA_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFS4975575.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="ACG8OA_000739"
     CDS             3684..4361
                     /locus_tag="ACG8OA_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4975576.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="ACG8OA_000740"
     CDS             4364..5491
                     /locus_tag="ACG8OA_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4975577.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="ACG8OA_000741"
     CDS             complement(5691..6131)
                     /locus_tag="ACG8OA_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4975578.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="ACG8OA_000742"
     CDS             complement(6142..8175)
                     /locus_tag="ACG8OA_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFS4975579.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="ACG8OA_000743"
     CDS             complement(8177..9880)
                     /locus_tag="ACG8OA_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4975580.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACG8OA_000744"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACG8OA_000744"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFS4975581.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="ACG8OA_000745"
     CDS             complement(10963..11988)
                     /locus_tag="ACG8OA_000745"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS4975582.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="ACG8OA_000746"
     CDS             complement(12197..13066)
                     /locus_tag="ACG8OA_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4975583.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="ACG8OA_000747"
     CDS             complement(13525..15417)
                     /locus_tag="ACG8OA_000747"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4975584.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="ACG8OA_000748"
     CDS             complement(15414..17141)
                     /locus_tag="ACG8OA_000748"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4975585.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="ACG8OA_000749"
     CDS             complement(17157..17807)
                     /locus_tag="ACG8OA_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFS4975586.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="ACG8OA_000750"
     CDS             18011..18307
                     /locus_tag="ACG8OA_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS4975587.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="ACG8OA_000751"
     CDS             18479..19099
                     /locus_tag="ACG8OA_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS4975588.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="ACG8OA_000752"
     CDS             complement(19191..20357)
                     /locus_tag="ACG8OA_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4975589.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="ACG8OA_000753"
     CDS             complement(20369..20722)
                     /locus_tag="ACG8OA_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS4975590.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="ACG8OA_000754"
     CDS             20850..21872
                     /locus_tag="ACG8OA_000754"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFS4975591.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="ACG8OA_000755"
     CDS             21891..22052
                     /locus_tag="ACG8OA_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975592.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="ACG8OA_000756"
     CDS             complement(22311..22745)
                     /locus_tag="ACG8OA_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975593.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="ACG8OA_000757"
     CDS             complement(22855..24714)
                     /locus_tag="ACG8OA_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4975594.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            24715..24855
                     /locus_tag="ACG8OA_000758"
     CDS             24715..24855
                     /locus_tag="ACG8OA_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFS4975595.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(24832..25992)
                     /locus_tag="ACG8OA_000759"
     CDS             complement(24832..25992)
                     /locus_tag="ACG8OA_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4975596.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ACG8OA_000760"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ACG8OA_000760"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFS4975597.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="ACG8OA_000761"
     CDS             complement(27739..28437)
                     /locus_tag="ACG8OA_000761"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS4975598.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="ACG8OA_000762"
     CDS             complement(28394..30055)
                     /locus_tag="ACG8OA_000762"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFS4975599.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="ACG8OA_000763"
     CDS             30273..31673
                     /locus_tag="ACG8OA_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFS4975600.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="ACG8OA_000764"
     CDS             complement(31925..33004)
                     /locus_tag="ACG8OA_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4975601.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="ACG8OA_000765"
     CDS             complement(33090..34049)
                     /locus_tag="ACG8OA_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFS4975602.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="ACG8OA_000766"
     CDS             complement(34061..35572)
                     /locus_tag="ACG8OA_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFS4975603.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="ACG8OA_000767"
     CDS             complement(35594..36157)
                     /locus_tag="ACG8OA_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS4975604.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="ACG8OA_000768"
     CDS             complement(36163..37026)
                     /locus_tag="ACG8OA_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4975605.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="ACG8OA_000769"
     CDS             complement(37040..37936)
                     /locus_tag="ACG8OA_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4975606.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="ACG8OA_000770"
     CDS             complement(38111..39400)
                     /locus_tag="ACG8OA_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4975607.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="ACG8OA_000771"
     CDS             complement(39372..40889)
                     /locus_tag="ACG8OA_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFS4975608.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT        11823 a         8589 c         7096 g        13495 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aat
//
LOCUS       DAZULO010000014        37550 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000014 DAZULO010000000
VERSION     DAZULO010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.014"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..172
                     /locus_tag="ACG8OA_000772"
     CDS             23..172
                     /locus_tag="ACG8OA_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975609.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            complement(206..370)
                     /locus_tag="ACG8OA_000773"
     CDS             complement(206..370)
                     /locus_tag="ACG8OA_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975610.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            665..1993
                     /locus_tag="ACG8OA_000774"
     CDS             665..1993
                     /locus_tag="ACG8OA_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFS4975611.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2195..3028
                     /locus_tag="ACG8OA_000775"
     CDS             2195..3028
                     /locus_tag="ACG8OA_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFS4975612.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3044..3781
                     /locus_tag="ACG8OA_000776"
     CDS             3044..3781
                     /locus_tag="ACG8OA_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFS4975613.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3936..4904)
                     /locus_tag="ACG8OA_000777"
     CDS             complement(3936..4904)
                     /locus_tag="ACG8OA_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFS4975614.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5136..5969
                     /locus_tag="ACG8OA_000778"
     CDS             5136..5969
                     /locus_tag="ACG8OA_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFS4975615.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            6003..6842
                     /locus_tag="ACG8OA_000779"
     CDS             6003..6842
                     /locus_tag="ACG8OA_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4975616.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6868..7428
                     /locus_tag="ACG8OA_000780"
     CDS             6868..7428
                     /locus_tag="ACG8OA_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4975617.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7627..9348
                     /locus_tag="ACG8OA_000781"
     CDS             7627..9348
                     /locus_tag="ACG8OA_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFS4975618.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9513..10655
                     /locus_tag="ACG8OA_000782"
     CDS             9513..10655
                     /locus_tag="ACG8OA_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS4975619.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10671..11882
                     /gene="thiI"
                     /locus_tag="ACG8OA_000783"
     CDS             10671..11882
                     /gene="thiI"
                     /locus_tag="ACG8OA_000783"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFS4975620.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12455..13102
                     /locus_tag="ACG8OA_000784"
     CDS             12455..13102
                     /locus_tag="ACG8OA_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS4975621.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13231..13447
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13495..16140
                     /locus_tag="ACG8OA_000785"
     CDS             13495..16140
                     /locus_tag="ACG8OA_000785"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFS4975622.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16450..17763
                     /locus_tag="ACG8OA_000786"
     CDS             16450..17763
                     /locus_tag="ACG8OA_000786"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFS4975623.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17753..18406
                     /locus_tag="ACG8OA_000787"
     CDS             17753..18406
                     /locus_tag="ACG8OA_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4975624.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18461..19012
                     /gene="radC"
                     /locus_tag="ACG8OA_000788"
                     /pseudo
     CDS             18461..19012
                     /gene="radC"
                     /locus_tag="ACG8OA_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            19143..20228
                     /locus_tag="ACG8OA_000789"
     CDS             19143..20228
                     /locus_tag="ACG8OA_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4975625.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            20225..22117
                     /locus_tag="ACG8OA_000790"
     CDS             20225..22117
                     /locus_tag="ACG8OA_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFS4975626.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            22124..22501
                     /locus_tag="ACG8OA_000791"
     CDS             22124..22501
                     /locus_tag="ACG8OA_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HFS4975627.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            22655..23437
                     /locus_tag="ACG8OA_000792"
     CDS             22655..23437
                     /locus_tag="ACG8OA_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HFS4975628.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(23563..24294)
                     /gene="erm(A)"
                     /locus_tag="ACG8OA_000793"
     CDS             complement(23563..24294)
                     /gene="erm(A)"
                     /locus_tag="ACG8OA_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HFS4975629.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(24352..24411)
                     /locus_tag="ACG8OA_000794"
     CDS             complement(24352..24411)
                     /locus_tag="ACG8OA_000794"
                     /inference="COORDINATES: protein motif:HMM:NF019858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HFS4975630.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            24803..25465
                     /locus_tag="ACG8OA_000795"
     CDS             24803..25465
                     /locus_tag="ACG8OA_000795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4975631.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            25700..25843
                     /locus_tag="ACG8OA_000796"
                     /pseudo
     CDS             25700..25843
                     /locus_tag="ACG8OA_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            25993..26193
                     /locus_tag="ACG8OA_000797"
     CDS             25993..26193
                     /locus_tag="ACG8OA_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4975632.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(26447..28126)
                     /locus_tag="ACG8OA_000798"
     CDS             complement(26447..28126)
                     /locus_tag="ACG8OA_000798"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS4975633.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(28128..28340)
                     /locus_tag="ACG8OA_000799"
     CDS             complement(28128..28340)
                     /locus_tag="ACG8OA_000799"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFS4975634.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            28733..30463
                     /gene="efrA"
                     /locus_tag="ACG8OA_000800"
     CDS             28733..30463
                     /gene="efrA"
                     /locus_tag="ACG8OA_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFS4975635.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            30460..32220
                     /gene="efrB"
                     /locus_tag="ACG8OA_000801"
     CDS             30460..32220
                     /gene="efrB"
                     /locus_tag="ACG8OA_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFS4975636.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            32315..32512
                     /locus_tag="ACG8OA_000802"
     CDS             32315..32512
                     /locus_tag="ACG8OA_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975637.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            32665..33150
                     /locus_tag="ACG8OA_000803"
     CDS             32665..33150
                     /locus_tag="ACG8OA_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFS4975638.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(33254..33847)
                     /gene="clpP"
                     /locus_tag="ACG8OA_000804"
     CDS             complement(33254..33847)
                     /gene="clpP"
                     /locus_tag="ACG8OA_000804"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFS4975639.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            34027..34554
                     /locus_tag="ACG8OA_000805"
     CDS             34027..34554
                     /locus_tag="ACG8OA_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975640.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            34645..35382
                     /locus_tag="ACG8OA_000806"
     CDS             34645..35382
                     /locus_tag="ACG8OA_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS4975641.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            35386..36303
                     /gene="miaA"
                     /locus_tag="ACG8OA_000807"
     CDS             35386..36303
                     /gene="miaA"
                     /locus_tag="ACG8OA_000807"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFS4975642.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            36305..37534
                     /gene="hflX"
                     /locus_tag="ACG8OA_000808"
     CDS             36305..37534
                     /gene="hflX"
                     /locus_tag="ACG8OA_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFS4975643.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        12593 a         6182 c         7863 g        10912 t
ORIGIN      
        1 atagctcagt tggtagtagc gcatgactgt taatcatgat gtcgtaggtt cgagtcctac
       61 tgccggagta aataaagctt atgcaaagaa aaagaaatcc aatcacttgg gtttcttttt
      121 cttttattat tatgatgaat caagctttct gtttttgtca cgtatgttgt aatctttacg
      181 aaagagtaga gttggctact cataatcaac catacttccc atcatcgcta aatacgaaat
      241 aaatcagcat tcctacacca atcaaagcac aagctgcacc aagaaaaggg atgacattcc
      301 caaagttatc tgtattcata agtatgccac cacccacaaa caaaaagccg ccagcaatca
      361 ggtttttcat tttattcatc tcctttctta cgttatagta tttccttctt tcaatgagta
      421 ttttgaaagc tatcttattg tatgatgttc ttctaggcct cttctttatt atacaggcca
      481 ttttcgttat ctcatagaaa tattcctttt tatctgaaaa ctattgtata ccgtatgaca
      541 tttttcttca tgaagttgta agtatattct cattcaaatg aaaatttcat catagaagag
      601 attgtatttc taaattctta agagtaaaat acgaagtata acttggattt ggaaggatga
      661 ttggatgaaa gctattacgc ctgatatgac aacaaaattt aacaaacagt ttcaagcaaa
      721 tatcaaacag caggcattgc agcgggcagt tgtgaagaac ggaattactg cttctgctga
      781 aaatgtttca gcacatgtgg agaatacacc ggtattttct atagatttgg ctacggggaa
      841 agtggctaat caaatgcaat cggggcgttg ctggatgttt gctgcactga acacattccg
      901 tcataagatg ctgaatcaat tccatttaaa agagtttgaa ttatcacaaa attacacttt
      961 tttctgggat aaatatgaaa aggcaaatta tttctatgaa aacatcattg caacaggaaa
     1021 agaggcaatc gatagccgga aagtagcttt cttactcgct actcctcaac aagatggcgg
     1081 ccaatgggat atgatcgttt cattattcca aaaatatggc gttgtgccta aatcggtgat
     1141 gccggaaagt gcgaatagtt caaattctcg agatttgaat aactacttga acaaattact
     1201 gcgtaaagat gcagtagtgt tacggaaaat ggttgctcaa ggagaaacat tagacgctat
     1261 tgaagaaaag aaagaagaaa tgctagaaag catttataac ttcttagcta tctcattagg
     1321 aacacctcca aaagaattcg attttgagta ccgcgatgaa gaaaagaact atcatcttga
     1381 ccgaggactg acaccacaaa ttttttatga taaatacatc ggtgtaaaat tagatgatta
     1441 tgttagcgtg atcaatgcac caacaaaaga caaaccattc aatcgttctt atacagtaga
     1501 gatgctagga aatgtcgttg gcgggaaaga agtcaaatat ctgaatgttg atatggaaac
     1561 tttcaaaaaa ttagcgattg ctcagcttga agaaggagaa tctgtttggt ttggttgtga
     1621 tgtaggtcaa tcttctacta gaactagtgg catcatggcc ttagatgctt attcaatgga
     1681 tgatctattt gatacggatt ttacaatgac taaagctgaa cgtttagatt ttggagaaag
     1741 tctgatgacc catgcgatgg tattgaccgg tgttgactta gtagacgggc aatcaacaaa
     1801 atggaaagtc gaaaatagct ggggcgaaaa agttggaaag aatggcttct ttgttatgag
     1861 tgatgcctgg atggacgaat atacgtacca aatcgttgta agaaaagatt tattgacgaa
     1921 agagcaatta aatgcgttca atgaagaacc aattgttcta tctccgtggg atccaatggg
     1981 tgctttagca tagacgagta tttcaatgaa gagtatgtag taaagataaa aagagctggc
     2041 ttaagccagc tctttttatc tttaaagtcc attccctgtt ttttatggtc cctgtgctat
     2101 aattctaaat aaaggataag gattggaaat aatcctttta tcattaaaaa tatgatgacg
     2161 aagtagaata aaattgggag taggggggaa agtcatggat atccaaactg ttttatctga
     2221 tctgaaatta aatggtaaag tgattccagt tgttggtggc gatgttaatc agacctatcg
     2281 tataaaaacc gaacatcgtg cttatttttt aaaaatccat ccgaatgtaa aaaaaggatt
     2341 ttttgaagca gaagtagacg gattaaaaga actgtcagca ttcgtacgtg taccggatac
     2401 gtatatgcta ggtgaaacaa gtgaaggcgc ttatttgtta atggaatgga tcgagccggg
     2461 aaaaggggat caacgagatc ttgctgcagc tttagctaat ctccatcagc aaacagcgcc
     2521 acaatttggt tttcgtaaag ataactattt gggtacactt gttcagaaaa attcttttga
     2581 agaagattgg tggacttttt tctttaaaga tcggcttgaa tcccagattt ctttggcaga
     2641 agaaacgaat cgatggaatg tacagcgtca agaaaagtat ctgcgattta aagaacgtgt
     2701 attgaaatcg gttgaaccaa aaaagatcac tccacgcctc ttgcacggag acctgtggag
     2761 tggtaatgtg ttctttgacc agcaagggca tccagttttt gtcgacccag ctgtttctta
     2821 tgggaatcgg gaacaagata ttgcaatgag ccagttattc ggtggtttcc gaccggaatt
     2881 tttagatgct tatcaaacta tatttccatt agaaaaggga tggaaagacc gactgccgat
     2941 ttaccagtta tattatcttt tggcacattt gaatatgttt ggagaaagtt acggttccca
     3001 agtagatcaa ttactagaaa atttttaaga ggagggcgtg tttttggcaa aaaaatatac
     3061 caaaaagcat gaagtttcat attatgagtg tgacatcaac caaacgatga catttccttc
     3121 gatgttagga gtagttatca aaacgtctga agatcaaagc gaccagttgg gaagaggcag
     3181 tgaattcgtc aatagctttg gacttacttg ggtcatcacg aattattcca tggagattaa
     3241 tcgtctccct agagtcggtg aagtaatctc agtaacgact caagccatgg agtacaataa
     3301 gtttttctgt tatcggaact tctggatcca tgatgaagat gggaaagaat tagtcaaaat
     3361 agaatctgtt ttcgtattga tggattttgt gaatcgaaaa atgagcagtg ttaatgaaga
     3421 gatcattgct ccttttgaga gtgagaagat caagaaaatc aagcgtcagg aaaaaattga
     3481 aaagattgat tcgggcttca tgttgccata tcgtgttcgt ttttatgata tcgatagtaa
     3541 tcagcatgtt aataatgcga tgtattttaa ttggatcatt gacgtattag gatatgactt
     3601 tttgactacg catgttccag aaaaagtaat tattcgattt gataaagaag tagagtacgg
     3661 gaatgagatc gaaagccatt ttgaacaaat ccctatcgaa aatggtgtca aaacaaaaca
     3721 tgaaatccga atgcaagaac agctgtattg tgaagcaaat attatatgga aaaataaata
     3781 aaaaagaggt tgtgacagaa gggggcaact tcaagaaata agaagaaatt tacgaaaatt
     3841 gtttctcaaa tttttgtaaa ttttcgactt agttttcgaa gaagctattt ttgtcacagc
     3901 ctctcttttt aagtttagct attagataat gatgtttagt tttggataaa cgaaatcaat
     3961 tcttcacccg acaaaccata gtttacagca atcaatagtt ttaaacgtgc tttttgacta
     4021 ttaatactgt tacagaaaat aatccccatt ttttctagtt cttttccccc gccttcataa
     4081 tcataaacag gctctgcgat tccattgaaa caacgagaaa caagtactac agggattttt
     4141 tggacaagaa gcttttccaa agcagctaag gcttgtggag ggagattccc cgcacctaaa
     4201 gcttcgatta ccaaaccgtc tactttcgta tgggcgatgg cttccaataa gtccccttgc
     4261 atcccagcat atgctttaac gatcgggata gtcccatcta cagcggaaat gtccagtcgc
     4321 tttgtttcta aaagttcctg caagaataaa attcgattct tagttactaa gccgatggga
     4381 cccaatgtag gtgttcggaa agtagcaaca tttgtggtat gggtctttgt cacgtagcgt
     4441 gcagaatgga tctcatcgtt catgaccacc aaaaccccct tatctagcgc ttcttcacaa
     4501 cttgcgacac gaatcgcact ttcaaaatta taaaggccgt cacttccaag ttcattactt
     4561 gaacgcattg ccccagtcaa aacaattggt aatttgttgc caatcgttgt atcaagaaaa
     4621 aaagcagttt cttctaatgt atctgtaccg tgtgtgatca ccactccgga tatacctgat
     4681 gcataagcgg acaagatacg ttcttttaat accaacatgt gagaaggcat gatgtgtggt
     4741 gagggaagat tgaagatatc ttcaactaat aattctacat gcttggggat tgtgatttct
     4801 gcatctaata atgggtttgc cacatcagga ctaacaccgc ccgtgagcat gttttctttc
     4861 atggcgatcg ttccgccggt gtgtagtaca agtatttttt tcattttaca gacttcctta
     4921 cgaataattt tgcatagccg ttaaatctta ttgtatacta tataaaagag ttttgctatc
     4981 agtgaagaat aacgaatagc ctaacttatc ataaaaaagc tgaccttatt aaaatggaaa
     5041 agactcaacc ttgacctcct aatacaatgg agtatctggt tgagtttctt ttacaagaaa
     5101 gcagccaagg aaatcaatca cgaaggtggc cggaaatgat cagaatcgga ttaacatcgt
     5161 ttaaagaaca tgacaaatta actggtaaaa agaataacac tctttatgaa tatgcagggt
     5221 ttctaccctt agtggaatta gatactgcct attatggaat tccccgaaag tcctcagttg
     5281 agaattggat aagtgaagta cctgaatcat ttcgctttgt agtcaaagtc tatcaaggaa
     5341 tcagcggtca aggagactgg aagccttatt atcagaccga agaagagatg attgcgcatt
     5401 ttttagatgt tatgtcgcca atggtagatg ctggaaagat gttttgcttt ttagtccaat
     5461 tcccctcatc attcaaatgt acgaaagaaa atgtccagta cttgcgcaaa cttagaaagt
     5521 ggtttgaggg ttatcctgtt tctatagaat tgcgggatta ttcttggtac agcgatgaat
     5581 ttcttgaacg tacaagaaac tttatgtcta gagccgcttt ttctttagcc atcatcgatg
     5641 agccacagtt gctgaataca acgattcctt ttgatccatt tgtcaccaat ccgaactttg
     5701 ctctttttcg attccatggt aggaatgcgc aaggatggca agcaaatgat aaagagtggc
     5761 gtaaaaaaag aacgttatat cgttattcag aaaaagaact gcaagaacta gcacatgaag
     5821 tcaaagtagt tgattctaag acaaaagagg taggtgtgat ttttaataat aattctggtg
     5881 gtgatgcggc tgaaaatgca ttagcactgc aaaaaatatt aaatatcaag ccagatggac
     5941 tcaatccaac ccaaatggat ctgttttaag ggcttatttt attcaagaaa ggggcgaagt
     6001 ctatgatcaa agctattttt tttgatattg acggaacatt ggtcaccaat cgttcaaaag
     6061 cgttggaatc aacaaaacaa gcaattgaat atgctcggaa aaacggcatt ttatgcggtg
     6121 tagcaacggg gcgcagccca gtaaagatca aagaaatcat tgatgaattg gaattagata
     6181 tgtatgtcgt gtataatggt caactggttt ttactgcaga tcgcacgatt atcgaccacc
     6241 cttttgaaca aaaagtattg gaacatattg ttgaatttgc agatgagaac catcgacaaa
     6301 tcgtttttgg tgcgagaaat cggttagatg gatcaacgac gatgctatta ggacaatcga
     6361 tttttatcaa gcgactagtc agctttttac cgcgtaaatt cccggtacgt ctgatgaaaa
     6421 aaatcctcca ggtttttagc cctcaccgac aaaaggatcg ttatgaaacg ttagagatat
     6481 taaaagaacc aatttatcaa tgtatattgc tcagtccgga gtcagaacag aaaaagttag
     6541 aacatcgctt tcccgagtgt acgtttcagc gttcaaatac gtatactgta gatatcatcc
     6601 caaaaggtgg ttcaaaatta ctaggcatcc aagctttcgc aaatgctgtc gaaattgaaa
     6661 tggaagaaat tatggcattc ggcgatcatt acaatgatat cgagatgctg aaaggtgtag
     6721 gaattggagt ggccatgggg aatgctcaga tcgaagtaaa acatgctgca gactttgtga
     6781 ctcaatcaaa tgaagaagat gggatatacg aaggactgaa acacttcggt ctgatttatt
     6841 agtcaatgaa agaaggaaaa aatcagtatg gaagcagaga caccttttca aaaagtagaa
     6901 acattccaca aaacttttga tccaagaaga ccagagatac ccacggcttt tacagaaaat
     6961 gaagctcata cacgtgctgc gttcaaaata gaagaacttg ttgaatttgt tgcagctact
     7021 tcacctaata aagaacaact gcatgaagct gttatgctgc tgcacgaagc attagataaa
     7081 gcagaagaaa aaatacttgg taaaacacag tggggaagtg atccgctggt tgaacaagta
     7141 gatgcactga cagatatcct ttactttact tatggttctt tttcattact aggaatagtg
     7201 cctgatcgaa tattcgaaat cgtccatcag gcaaatatgg ggaaattatt tccagatgga
     7261 aaacctcatt atgaccctgt aacaaataaa gtgttgaaac ctgaaaactg gcagatggaa
     7321 tatgctccag aacctaaaat cgcggcagaa atccaacgtc agattcaaga agcgattgct
     7381 aagaagaaat ccgctgatat aggcatgtgc acaaatgaaa aagagtgatc agagtttcac
     7441 tatttttgtt atctttatac gatggaaaaa caaaagattt tcgtttaagt tagtgaatgc
     7501 atgaaaatag tatgctataa tctagaatag atgtcttttg cacgagagac gtattatttg
     7561 gatttatgcc aagcagaacg ccatctgttg cataaaagat gcatactgaa aacggaggaa
     7621 ccataaatgg gaaataatgt tatcatcgga atcgtcattg caatcattat agtcgccatt
     7681 gtcgtctttt tggttagtta cttgatgaag aaaaaaaacc aagatcgatt ggatgcctta
     7741 gaaaaacgca aggaagaatt atttgacttg ccagtgattg aagaagtcga tgatgttaaa
     7801 aaaatgcatc tggttgggca aagccaaaat acctttcgtg aatggagtca aaaatggaat
     7861 gacatatcta cttcctcgtt cgctgaacta gaaagtcaaa ttttcgaagt ggaaaacctc
     7921 aatgaaagtt ttcggttctt aaaaggaaaa aaagcagttg ttgaagcaga agctacaatg
     7981 gaccaaatgg agaaacaagt gacggaaatc cgtgaaggtc taaaggaatt gcgggaaacg
     8041 gaagaacgca actcactagc tgttcagcag gcacttgacg tctatgaaga actgaaagaa
     8101 aaagtcagag aagacgagca tgaatacgga ccagctcttt ttgaattgca aaaacagatc
     8161 aaagtgatcg aaaaagaatt tacgcagttt gtgactttaa acacttctgg agaccctgtt
     8221 gaagcccgcg aggttttgga taaagcagaa aaacatacgt atgaagtgga agctctgatg
     8281 aaaaaaattc caccgctgta tgaggagctc cataccactt tccctgaaca acttgaagaa
     8341 atcagtgata cttacgagaa attgatggaa gaacaatatg tattcccaga agaagatctt
     8401 gctgaagata tcgcaaaagt aagtcgaaga attgaaaatt cattagccaa tcttgaaaaa
     8461 acagaagttg aaacggtcga atttgaaaac cgtgaaacag ctgatttgat tgattcgtta
     8521 tatgatatct tagaacggga aatggaagct cagagatatg taaaaactaa ccaatcaacg
     8581 attgcagagt atatcaaaca tacaacaaag aataaccgtc aattattgat tgagctggat
     8641 cacacatctc aaagctatac attgaaccac aatgaaattg gccgtgtacg aggtttccaa
     8701 accgaagtgg aagaaatgga acgtcaaaat gaacagatga ttccacagat ccgtcaacac
     8761 gaaattcctt attcagaagt gcgcacgttc tataaaacag tcttcaaagt cttagaagat
     8821 atcgaaaagc aacaagtaga gatcgacgac tcattacatg aattgagaaa aggcgaaaaa
     8881 gaagcgcaag aaaagatcga tacattcgaa ttcaaattgc gcagtttgaa acgttttgta
     8941 gaaaaacaac gtttgcctgg cttgccgaat gattacctag aattcttttt tgttgctact
     9001 gaccgtattg aagaactaag tgttgtcttg aataaaattc gagtaaacat ggaagaggtc
     9061 aatcgtttag ttgctctatg tgaagaagat ctagaattat tagataagaa gacacatgat
     9121 ttagtagatg cagcagcatt aacggagcaa atgctccaat atgcaaatcg ttatcgtcat
     9181 acacacgaag aagtgagaga agcaattgat aaaagctact atttattcaa taaagaatat
     9241 cactatcaag aagcattaga cgaaatcggt acagcattag agcgagtaga acctggtgct
     9301 ttcaaacgca tcgaagattt ttattttaat catccagact tagtataaag caaaaaagtt
     9361 agtaaaccag caagagcaag acggattatt gtcttgttct tgtctttttt tgtatatttg
     9421 aaaattgaaa caagtgaaaa tttcacctgc tcttgaaagc accttggaca accgttataa
     9481 tagaaaagaa tttcaaaaaa gaggggtttc ttatgatata ttttgataac agtgcaacaa
     9541 ccccaatctt tccgcaaagc ttagatgcat atgtaaaaac cagtcagcgg attattggga
     9601 atccatcaag cttgcatgat ttaggcaatc aagctaaccg gttgttgcag caagcaagaa
     9661 agcagatcgc tgatttgatc catgtagaac cagaagaaat ctattttact tcaggcggaa
     9721 cagaaggaga taactgggta ttaaaaggaa cgatgatcga aaaaagagaa tatgggaatc
     9781 atatgatcat ttcaggagtc gaacatccgg ccgtcagcga gacagcagaa caattaaaag
     9841 aattaggaat cgaagtaagt atcgccccag tggataaacg cggatttgta cgtgtcgacg
     9901 aattgaaaga attaataaga aaagagacag tattagtctc tgttatggct gtgaataatg
     9961 aagtaggatc gattcagccc atccaggaaa tcagtgatct tttggatgat tttcctaaaa
    10021 ttcatttcca tgtagatgct gttcaggcga ttggaaaagt accattctcg caatggttga
    10081 cagaaagagt tgattttgca acattttcag ctcataaatt ccatggccca cggggaactg
    10141 gtttcattta ttggaaaaag ggtcgtcgtt tagcaccatt actgacaggt ggagggcaag
    10201 aaagaaatca gcgaagtggg acagaaaatg ttccaggtat tgtggctatg gttaaagctt
    10261 tacgtttatc attagagata aaggaacaaa aacctgagca gacacaacgc ttaaaagtag
    10321 ctctaatcaa tgcgttgaag acgtatgaga aagtgacgat tttttcagaa ggaactgact
    10381 atgcaccgca tattttatgt tttgctctaa aaggaattcg cggcgaagtt ctcgtccatg
    10441 ccttagaaga aaaacaaata tttgtttcta ctactagtgc ctgctcctca cgtaaacata
    10501 tggcaagcag tacattgcac gcaatgcatg taccaaatga tctggctaca tctgctgttc
    10561 gaatcagctt agatgaaaac aattcgcttg cagaagtcga gcaatttatg attattttca
    10621 agcagcttta taagaaattt tctgcaatca attaaaagag gagtgttctt gtgaaataca
    10681 gtgaaattat ggtccgttat ggggaacttt cgacaaaagg aaagaatcga aaaagcttta
    10741 tcatgcaact agcacaaaat gtcagacaag ctttgaaaga ctttccagaa atcaaaatcc
    10801 atgctgatcg ggaccggatg catcttttat tgaatggcgc agacgggcag ctagttattc
    10861 caaaactagc aaaaatattt gggatccaaa atttctctcc aagtattcgt gtagaaaaag
    10921 atgtcgaagt tttgaaacaa gctgttcaag acatcatgaa agaaatttat actgggaaag
    10981 aaacatttaa aattatcgct aaaagaagcg atcaccaatt tgaattgaca agtaatgaat
    11041 tgaatcaaac attaggtaat gctgtttttg atattttccc gcatatccaa gttcaaatgc
    11101 gtcaaccaga tattccatta cgtgtagaga ttcggcgtga tggcgcttat ctatcgtatg
    11161 aaacgatcaa gggagctgga ggattgcccg taggaacaag cggaaaagga atgcttatgc
    11221 tttctggggg gattgattct cctgtagcag ggtactttgc catgaagcgg ggagtggaaa
    11281 ttgaagctgt tcattttgct agtccgccgt atacaagtga acaagcactg caaaaagcaa
    11341 aagatctaac agccaaacta gcaccttatg tgggaacgat ccattttatc gaagtgccat
    11401 ttaccgagat tcaagaagaa atcaaaaagt cagtgccaca aggatattgg atgacaatta
    11461 ctcgtcgaat gatgttgcgt ttgaccgatg aaattcgccg tattcgtcat ggtttagtca
    11521 ttttgaatgg tgaatcatta ggtcaagtgg cttcacaaac tttgcaaagt atggtggcta
    11581 tcaatgaagt aacgaatacg ccaatcattc ggcctgtagc gacaatggat aaacttgaaa
    11641 tcattgaagt agcagaacag atcgatacat ttaatctttc tatccagcct tttgaagatt
    11701 gctgtacgat ttttgctcct ccacagccaa aaacacgccc tcaactggaa aaggtccatc
    11761 aatatgaaga gcgtttagca atagatgaaa tgatccaacg agcactagct ggcttgaaga
    11821 tggaagagat acaagcagaa tcgtctaatg aaaaaactga agaattttca gaattgctat
    11881 aagaggttat gaaaatagtg ttttgaccag agtattagag aaacaaaacg aaaaaatagc
    11941 tccatgtatt ttaccgtttt tgaactaatg tcgaagatta gctgattgaa taccatttat
    12001 caagaagtta tggaaaagcc ggttcttttt tactaactga aaaataaaat tttatttaga
    12061 gatcaagcta cagttgataa atgatataaa aaagagaatt tcccatgaaa tagcgaggtt
    12121 gtgacagaag tgggcagctt caagaaataa gaaggaattt acgaaaattg cttttcaaat
    12181 ttttgtgaaa tttcggctta tttttcgaag aagttacttc tggaacaccg tgtatcagag
    12241 gttgcgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
    12301 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgttttattt
    12361 gtctcacacc cttttccttc ctacttgtaa ccttgctccc gtgttagaaa catgttacac
    12421 taaatatgtg aaattattaa caaggagcgt cgttatgcga gaaaaaacag atacactgcc
    12481 aaaagcaact gcgaaaagat tacctttata tttacgctac ttgaagatgc tggatgactc
    12541 aggtataagt agaatcaaat caaatgagtt cagcgaaatc acccaaattc cttctgcaac
    12601 gatccgcaga gacttctcgc aattaggcga gctaggcaga agcggatatg gctatgatgt
    12661 cccttttctt atcgatgtat tcaataatat ccttaatact aaagaagaaa aacgaatcgc
    12721 attagtagga tatggaaatc ttggcaaagc attgaaacac aacaatttta gaagaaatga
    12781 aaatctaaat atcgtgtgcg tctttgataa tgatccagca ctgatcaatc gagtaattga
    12841 tggagaaatg atctatccga ttgatcgttt cgcggagata gccaaagcaa aaaatgtgac
    12901 agtagcaatc tctacggtac caagtaaata ttcacaatct gctattgatg aaattgtcaa
    12961 aggaaatgta accgctatac ttaattttgc gcctgacaga gtaactgtcc cagcttatgt
    13021 caatgttcaa tacattgatt tgacaaccga gttgcagacg cttatttact ttgatgagaa
    13081 ttactcagaa gttttttctt aggaaaattt gagtaggctt attgatcttg cgagaactcg
    13141 tcgtaattct ttcaaaaatt ttttagtact aaatgaagtg gttttttcac gaccacttgt
    13201 tgactaaaaa aaaaaaccat tgtagaatga agcataacgc tgaacaagag gagtaactgg
    13261 cacattcatt cttagagaga gaactatctg ctgaaaggtt ctcaatgaca accagtgaag
    13321 gtagcttggg agtttctgtc ttgaatggag tagagataga cgagtcgtga tcgttaactc
    13381 acttgagagg caataatgat tgattattgc aatttaggtg gtaccgcgta aatcatacgt
    13441 cctagaatac gaaagtattt taggacgttt tttagtttaa aggaggaaaa cagcatgacg
    13501 gaagaaaata acctatcaac aaaatataat ccgcaagaag ttgaagctgg acgttatcag
    13561 cattggttag aacaagattt gtttaaaccg agcggagata aaaaagcaaa accttattca
    13621 atcgtaatcc cgcccccaaa tgttactgga aaacttcatt tagggcatgc ttgggatacg
    13681 accttgcaag atatgctgat tcgtcaaaag agaatgcaag gatatgatac tttatggctg
    13741 cctggtatgg accatgcagg aatcgcgaca caagcaaaag tagaagcgaa gcttagagag
    13801 caagggattt ctcgttatga tctaggtcgt gaaaagttca tcgatcaagt gtgggaatgg
    13861 aaagaagagt atgcgagcca catccgcgaa cagtgggcta aaatgggctt atctttagat
    13921 tacagtaggg aacgttttac attagatgat ggattatctg aagcagtacg taaagttttt
    13981 gttaccttat acgaaaaagg tttgatttat cgtggtgaat atatcatcaa ctgggatcct
    14041 caagcacgaa cagctctatc tgatatcgaa gttattcata aagatatcga aggcgccttt
    14101 tatcatatga gctatgaact agcagatgga tcaggcgtag tagaaattgc gacaacccgt
    14161 ccagaaacga tgttgggaga tacggcgatt gctgttcatc cagaagacga gcgttatcaa
    14221 gcgctcatcg gcaaaaaagt gattctacct ttagtcaata aacaaatccc aatcattgca
    14281 gatgaatatg tagatatgga attcgggaca ggtgtagtaa aaatcactcc agcacatgat
    14341 ccaaacgact ttgaggtagg taatcgtcat gatcttccac gcgtaaatgt tatggatgat
    14401 gatggtacga tgaatgaact agcaggaaaa tatgctggaa tggaacgttt tgctgctcgt
    14461 aaagcaatcg tgcaagatct gaaagaaatc ggtcgattga ttaaaatcga tacgatgatc
    14521 cacagtgtgg gtcattccga gcggacaggt gtcgttgttg aacctcggct gtctactcaa
    14581 tggtttgtaa aaatggcgcc gttagcgaaa aaagccattg ataaccaaga aacagatcaa
    14641 gcagtggaat tcttccctcc aagattcaat caaacgtttt tacgttggat ggaaaatgtc
    14701 catgattggg taatctcacg acaattatgg tggggacatc aaattcctgc atggtatcac
    14761 aaagaaacag gtgaaatgta tgtagggatg gaagcaccag cggatagtga aaactgggta
    14821 caagatcctg atgttttaga cacttggttt agttcagctt tgtggccttt ctcaacaatg
    14881 ggctggccag atacggaaag cgaagattat aagcgatact tccctactag cactttagta
    14941 accggttatg acatcatttt cttctgggta agcagaatga ttttccaaag cttggaattt
    15001 actggcgaac gtccatttga gaatgtcttg attcatggat tgattcgtga cgaacaaggg
    15061 cggaagatga gtaaatcatt aggtaatgga atcgatccga tggaagtaat cgaaaagtat
    15121 ggagcagatg ctctccgctg gttcttatcg aatggttctg caccaggaca agatgtccgc
    15181 ttcagctatg aaaaaatgga tgcttcttgg aactttatca acaaaatctg gaatgcttca
    15241 cgtttcgtta tcatgaatat cgaaggaatg accgtagaag agatcgattt atctggagaa
    15301 aaatcagtgg ctgaccggtg gatccttact cgtttgaatg aaacgatcga acgagtaact
    15361 gagctatttg atcgttttga atttggtgaa gcaggtcgac aactgtataa ctttatctgg
    15421 gatgattttt gtgactggta catcgaaatg agtaaagaag tactttatgg agatgatgct
    15481 gtttcaaaac aaaccacccg tagcatctta gtccatacac ttgatcagat cctacgttta
    15541 ttgcatccaa tcatgccatt cgttacagaa gaaatatggg aacatattcc tcatcaggga
    15601 aattctctag tagtggcaga atatcctgtt gtacgtccag agtttgatga tgaaacagct
    15661 tctaaaggga tggaagtatt gaaagaattg attcgagccg ttcgaaatat tcgttcagaa
    15721 gtgaatacac cattgtctaa accaattacg ttgctaatta aaacaaacga tgctaaaatc
    15781 gatcaatttt tagttgaaaa tacaagctat atcgaacgct tctgtaatcc cgaagagttg
    15841 acgatatcaa gtgagatcac tgcgccagat ctagcgatgt cagctgttct gacaggtgca
    15901 gaaatcttct tgccgttagc aggattgatc aatattgaag aagaaatcaa acgattagaa
    15961 aaagaactgg aaaagtggac aagcgaagta aaacgtgtcc aaggaaaact ttctaatgaa
    16021 cgttttgttt ctaacgcgcc ggaagaagta gtagaggcag agcgtgcaaa agaaaaagat
    16081 tatttagaga agcaaacggc tgtcaaagaa cgaatcgaaa gtttgcgtac tattcaataa
    16141 gaatagaaaa agagagtgtg aaatcagcta gctattacta aaagctagct gattgaacat
    16201 cgttacagga aagagtttga gcctcactct tagcttcgat tcataaggag atgtgacata
    16261 aattttgtcg catctctttt ttagtctact cttattatgt aggagaagga gggaggtgga
    16321 tcatgtatca agagtcaata aagtagttga taaaatcgtt cctctctctg tttcttttct
    16381 ctttgtcata cttttatttt tcttatgtgt tctatttgtt ataataaaag agaaatgggg
    16441 gaattgttga tgacgattga agaagcaata gaatggatcc atagtcgatt accatttggt
    16501 tcacgtcctg gccttgaccg agtcgaagct ttattagaag tggtagggca tccagagaga
    16561 aaagtaaaaa cgatccatat cgctggaaca aacggaaaag gttctactgt agcttatttg
    16621 agaagcctat tagaagaggc tgggctgaca gtaggtacat ttacttcacc ttatatcgaa
    16681 acgttcaatg aacggatcgc aatcaatggc aaaagcattc cagacaatga tttgatccag
    16741 cttgtaaaaa aatatcagcc actgatcaaa caacttgatc aatttgaagc agtagctggt
    16801 attactgaat tcgagacatt aacagcaatg gcatttgatt attttttgat gaagaaagtg
    16861 gacgtagcga ttatcgaagt tggcttaggc ggcttattgg attctaccaa tgtagctgta
    16921 ccaatgctga cgggaataac aacgattgga cttgatcata ccgaaatact tggagataca
    16981 attgaagaaa tcgctgcaca aaaagctgga atcatcaaac aaggcgttcc agtagtaaca
    17041 ggaaatattg ccccagaagc ttttactgtc atccaacaaa cagctaaagc aaaaaatgca
    17101 ccgctttatg tatacgggga aaattataaa ataaactatc tgcatcctga tgagatgtgg
    17161 ggagaagtat ttgaattttt taatgaaata ggaaagatcc ctcaattaac cactccgctt
    17221 ttagggcaac atcaagctga aaatgcagga gtagccatcg aattgtattt tgtttactgt
    17281 caattggaaa agctgccttt ccgagaaaaa gatgttttga agggattgaa gaaatctcga
    17341 tggccaggaa gaatggagcg tttgagtaac gagccattag ttgttttaga tggtgcccat
    17401 aatacgcatg caatgaaccg gcttgttgag aatttgaaaa aagaattcaa aggatatcaa
    17461 atcaacattt tgttttccgc cttacgaact aaaaatgtta gagaaatgct agaacgattg
    17521 cagcaagtac caaatgtcca tatttatttg acgacctttg aacatccaaa agccatcgaa
    17581 ttaagtgaag tatcagattt agcaagtgag agagtatcaa tcgtttcatt gtggcaattc
    17641 ggattagcag atatattgga aaagatgaca aatgaagacg tattagttgt gaccggttca
    17701 ctttatttca tttcagaagt tcgccattta ttaatacaat taggaggaga tcatgaagta
    17761 taatgcagtg atttttgaca tggatggttt gctgttcgac acagagttag tgtattacga
    17821 agccacacaa gtagtagcag acaagatggg ttttccttat gataaagacc tgtatttaaa
    17881 attcttaggt gtttcagatg aagaagtatg ggcgaattat catatgattt tctcggaatt
    17941 tggcaaaaca agtgtacaaa gattcataga tgattcatac caggaaacgc ttgaacgttt
    18001 ttcgcaagga gcggtacaaa tgaagcccgg tgtgttggaa ctcctctctt ttttagaaga
    18061 acatcagatt cccaaagttg tcgcttccag taaccaacga gcagtcattg aacttttatt
    18121 gaagaagaac aatctgtttg aacgatttga gacgattgtt tctgcagaaa atgttcaacg
    18181 agcaaaacca gacccagaaa ttttcttgtt agctaatcag aagctaggta cagcgaagaa
    18241 agaaacattg attttagaag attcacaaaa tggtatttta gcagcttctg cggctgagat
    18301 cccggtagtc atggtaccag atctgcttcc accatcccaa gaattgatca aaaaaacagc
    18361 tggggttgtt tcttctttac atgaaatccc tgcattgtta aaataaaaat ccatctcttt
    18421 tcgtatctta ttttagataa gatagaaagg agatgttttt ttgggaaaac gattgatcaa
    18481 agaagtacct actagctctt taccaaggga acgtatggaa atttatggcg cggaagcttt
    18541 atcggatcag gagcttttgg caatcttact gcgtaccggt caacaccctt atagtgtgat
    18601 gtcgattgct ggaaatctgt taaaaacgtt tggaggactg gcctctttac gacaggctac
    18661 tttgcatgag ttagaagaga ttcgagggat aggacgcgtg aaagcaatcg aaatcaaagc
    18721 cttgatcgaa ttaggtcgac gaattcagac ggatcatgaa agagcggctc caccggttcg
    18781 taccagctac gaattggccc agcaattgat tctggaaatg aaagatcaca aacaagaaca
    18841 tttggtttgt atttatttgg acacgaagaa tcaagtgatc ttaaaaaaaa cagtgttcat
    18901 cggttcgctg aatcagtcag tcgctcatcc tagagaaatt ttccattatg ctgtacgtta
    18961 ttgtgcagca aggatcgttt tagcgcataa tcatccttct ggtaatgttt taaccatagt
    19021 ataataaata atagtagtta aagtagttta agaggagata ttaaagggat attttacttt
    19081 aaataaaaat taacaaggtt tataatttgt agtagttaaa gtatttaaag aggtgggaac
    19141 atgtgaaggt tcaaaggata gaagtggaga ataagccgta tccattgtat ttattactag
    19201 ataaagaata ccagctaata gaaccagtaa tgaaatttat taaatactta gataatactg
    19261 gtaagtctcc taataccatt aaagcatact gctatcattt aaagttgctg tacgagttca
    19321 tggaacagag aggtgttatt cttaatgata ttaactttga gttgttagca gacttcgtag
    19381 gttggttgag atatccttca gcatcaaatg taattgatct tcagtcaaaa aaagccataa
    19441 gagaagaaac gacagtgaat acaattttaa atgtagttat gagttttctt gattatttaa
    19501 gtagattagg agaatttaaa tcaattgatg tatttaaaca agcaaaggga agaaatttca
    19561 aaggattttt acatcatgtt aataagggta gataccaaaa gaatgtctta aagttaaggg
    19621 ttaaaaagaa acagataaga acattgagat cagaggaagt taagcaaatt attgatgctt
    19681 gtcatacgaa aagagataaa ttaattttaa tgcttatgta tgagggtggt ttaagaatcg
    19741 gtgaagtgtt atcgcttagg cttgaagata ttgtcacttg ggacaatcaa atccatttaa
    19801 cacctagaga tgttaatgtt aatgaagctt atattaaatt aaggaaggaa agaacaatac
    19861 atgtgagtaa agaacttatg tcactttata cagattactt gatatatgag tatagtgagg
    19921 aattggagca tgattatgtt tttatttcct taaaagaagg ctattttggg aaaccactaa
    19981 agtaccaaag tgttcttgat ctagttagaa gaatagttaa aaggactgga atagaattta
    20041 catcacatat gcttcgccac actcacgcaa cgcagctaat tagggaagga tgggatgttg
    20101 cgttcgttca aaagagatta ggtcacgcac atgttcagac aacgttaaat acctatgttc
    20161 atctttcaga tcaggatatg aaaaatgagt ttaataaata cctcgagaga aaggagcata
    20221 agaaatgaat gcttctagta aaaggaaaat tattagtcag agtgagatta gcaaaaaaat
    20281 agctgtaatg aatgaagaaa tgcaggggtt ttgggctaat aatagttggg atataagaaa
    20341 atgtccacat ccttctgcca tagaattaag taagaatcct gctttaagga atcgttgggt
    20401 tcgttttgaa cgtgttaaaa atctgtggtt aagaacagaa ttgaaatatt tttattttta
    20461 ccatttaaac aatggaatat ggaatgcaaa aactgtctgg attagaaaag gaacagtaat
    20521 taataaaatg ttagatttct tagatttaaa gtatcctagc attacttcaa ttactgaagt
    20581 tcctattgaa aaagcaatga cggagtatag aacttatttg acaaaacggg gtgttagaat
    20641 taccactact aattataaga ttactgctaa tcaagaaaaa acacctgtaa aagctaattc
    20701 ctactatgtt actaatctaa aacaatttat ggagttttat gagaactttt attttgatgg
    20761 agaggagtgg gataaagacg tttgggatag acgtaactta cctttgccag atgataaggt
    20821 taacccaaca caatatgaat atacaattaa ctttaaaggg tttcggaata catattttaa
    20881 acaacttgta aaaagatatt gtaagttgag attgaacatg gatagctttt cctatgtaag
    20941 tgatattgcc caaagactta aagagttctt taattttctg gacatgaaat ttaaacaagt
    21001 tcagagagta caccaattaa cgagagtgga aattgaagca tatttaagtg aactaaacat
    21061 gatgggaata aaacctagta caataactgg gaggatctct atattggaag gactatttag
    21121 tacccttctt aggctagaat gggatgatgt tccttccaaa atattaattt attctgagga
    21181 ctatccgaaa ataccaagag caaaaccacg ctttatagat gaattcgtcc tagagcaatt
    21241 gaacagtcat cttgataaat tacccgaata tatagctacg atgactatga ttgttcaaga
    21301 atgtggaatg aggataagtg aattgtgcac cttgaaaaaa ggctgtctat tagaggacaa
    21361 agatggagat ttctttttaa agtattatca atggaaaatg aaaaaggagc atatagttcc
    21421 aatatctaaa gaggtagctt tacttattaa agttcgggaa gataaagttt cagaggaatt
    21481 tccagatagt gaatacctct ttccaagaaa agatggatcg ccattaaaac aagaaacatt
    21541 tagaggtgag ttaaataaat tagcttatga gcaaaatata gtggataaat caggtgagat
    21601 ttatagattc catgcccatg cctttcgcca tacagtagga acaagaatga ttaacaacgg
    21661 gatgccccag catattgtgc agaaattttt ggggcatgaa agcccagaaa tgacaagcag
    21721 atacgctcat atctttgatg aaactctaaa aaatgaattt actaaatttc aggaaaaact
    21781 ggttaccaat aatggagatg tgcttgatct agatgaagat aatgaagtcg atgatgtaga
    21841 gcttcaatgg ttcaagaaaa atataaatgc acaagtgctt ccaaatggtt attgtagatt
    21901 gccagtagta gcaggtggtt gtccacatgc gaatgcatgc ttagattgca ctcacttctg
    21961 taccagtaag caattcttac cacagcacga agaacagtta gagcgtacag aagagttatt
    22021 agccatagct aaggataaac aatggcaaag acaagtagag actaatagcc gtgttaaaga
    22081 gcgtttagaa caaatcattg gaagtttgac ggggtaatta tcaatggata aacaagttag
    22141 aaatacaaca gaaattgtac gtttggcgaa gcagaaatca aaaaagacaa gggaaaaagt
    22201 agacaaagcg atttctaaat tttcgattga aggtaaagtt attaatttta attcaatagc
    22261 aaaggaagct aatgtttcta aatcatggct ttataaggaa cacgatatta ggcaaagaat
    22321 cgaatccctt cgtgagcgtc aaataacagc aaatgtagtc tcaaaaccca agaaaagttc
    22381 tcgttcggag gaaatcctta ttaaaacctt aaaaagaaga gtaatggaat tagaaaaaga
    22441 aaataaaaaa ttacagaacc aaattcaaaa attatatgga gatctgtata ataaagaata
    22501 attattaatc tgtagacaaa ttgtgaaagg atgtacttaa acgctaacgg tcagctttat
    22561 tgaacagtca tttaggtata tgtccaatct agggtaagta aattgagtat caatataaac
    22621 tttatatgaa cataatcagt tacgaggtga aatcatgagc aatttgatta acggaaaaat
    22681 accaaatcaa gcgattcaaa cattaaaaat cgtaaaagat ttatttggaa gttcaatagt
    22741 tggagtatat ctatttggtt cagcagtaaa tggtggttta cgcattaaca gcgatgtaga
    22801 tgttctagtc gtcgtgaatc atagtttacc tcaattaact cgaaaaaaac taacagaaag
    22861 actaatgact atatcaggaa agattggaaa tacggattct gttagaccac ttgaagttac
    22921 ggttataaat aggagtgaag ttgtcccttg gcaatatcct ccaaaaagag aatttatata
    22981 cggtgagtgg ctcaggggtg aatttgagaa tggacaaatt caggaaccaa gctatgatcc
    23041 tgatttggct attgttttag cacaagcaag aaagaatagt atttctctat ttggtcctga
    23101 ttcttcaagt atacttgtct ccgtaccttt gacagatatt cgaagagcaa ttaaggattc
    23161 tttgccagaa ctaattgagg ggataaaagg tgatgagcgt aatgtaattt taaccctagc
    23221 tcgaatgtgg caaacagtga ctactggtga aattacctcg aaagatgtcg ctgcagaatg
    23281 ggctatacct cttttaccta aagagcatgt aactttactg gatatagcta gaaaaggcta
    23341 tcggggagag tgtgatgata agtgggaagg actatattca aaggtgaaag cactcgttaa
    23401 gtatatgaaa aattctatag aaacttctct caattaggct aattttattg caataacagg
    23461 tgcttacttt taaaactact gatttattga taaatattga acaatttttg ggaagaataa
    23521 agcgtcctct tgtgaaatta gagaacgctt tattacttta atttagtgaa acaatttgta
    23581 actattgaaa atagaaagaa attgttcctt cgatagttta ttaatattag tgacatttgc
    23641 atgcttcaaa gcctgtcgga attggttttt agtgaaaaga acacgatatt cacggtttac
    23701 ccacttataa acaaaagatc gatacttttt gtagtccttc tttgaaatca atggttgatg
    23761 tcgttcaaga acaatcaata cagagtctac acttggctta ggatgaaaat atagtggtgg
    23821 tacttttttg agcattttta tatccatctc caccattaat agtaaaccca aagctcgttg
    23881 cagattttgc aatcttttcg caaatccctt ctcaacgata agatagctat atttagcctg
    23941 actttcaaag gtaattcttt tgacaatatc cgtactgatg ttataaggaa tattaccata
    24001 tatcttatag tttatatgtt ttgggaagga aaattttaga atatccgttt gaatcacttt
    24061 tatattctca gaggggttta ccgcttcttt agtcacttga cataagcctc catcaatttc
    24121 tatagcagta actgatcgac tcattttgac tagctctttg gtaaaatgtc cttttcctga
    24181 tccgatttct attacgttgt cttgtttact gatattcgtg tgattcaata tttcttttac
    24241 atgcttttta gaagtaataa aattttgcgt gtctttaggg tttttctggt tcattataac
    24301 cttctcctta ctggttataa tgaactgaca ttaagcgttc attataacca attaattttg
    24361 atttggttga taacgaactt tattaataac aaatatagaa aaagtaccca tacttttatc
    24421 tcctttccat ttttttcatt atgaatgaga taaagtaatt tctactactg cgatactggt
    24481 gcacataatt gaaacctcct tatgtaactg aatatgattg tatcatcttt tcattttaga
    24541 ataaaccttt atatgtaatt ttgcacttga cacttcagat ggacgttttc ttcaaaataa
    24601 tatgttttga aacttgataa gagaagccac tgtctttact aaaaagtaaa ggctgcgata
    24661 aaggacagtg actttaataa agttgcactg ttgtattgtc tttggataaa attgtctaaa
    24721 gggaagtttt atccccaacg aaaaaacatc taaagtggta agtaatgaaa gaatgtcgat
    24781 aaagtaggaa tgggggatct ttttgaatac aatagaatca cagtttgata aggttgcaga
    24841 agattacgat ttcgtgaatg agcttttgaa tgattattca ttctttgtgt ctaatatgtc
    24901 tccaaagaaa ggcagagcat tagatatcgg atgtggctcg ggtttgttag tggagaaatt
    24961 agcaagttat tatgatgaag tggtagggat tgatatttct aatcaaatgc tcgatcttgc
    25021 caaatctaaa cgtcaactaa caaatacggt ctatctgaat atgaatgcag aacaacttaa
    25081 ttttaatgag aagtttgatt ttattgtaag ccgaacaacc tttcatcatt tggatgatat
    25141 agccagcgta atacagcaaa tgaaggaact gttgaatgag gaaggaagaa tagttattct
    25201 tgataatgta tctgaagtcg agacaccgcc tacctatgtt tataaattag gggcaatcca
    25261 agagttttta ccgcattgtt tcaaatttgg aataaaaaat gcgattagaa tttataatca
    25321 caacacatct aaatcatggc ttgagcattt agcttcagat aagtacctat ctgaacaaaa
    25381 ttattacgat ttatatgaaa agttgttgcc tggatgccaa ttccataaaa tgggttgggc
    25441 aatgggggtt gtctggacaa aataacttaa agctgtggct ttgaaaagtt gaatttgtga
    25501 ttgttttcag agctatcggg tgctttagta taaataacaa tgaataaaaa atcagcctgt
    25561 atcttatgtt actattaagt aatttaagat acaggtttta ctatttttga aataagtacc
    25621 ttccacttca aatacatgta ttcaaagtga aagtattgat ttaaatgatt cttttgtaat
    25681 ttactcttga taatatgaaa ttccatcgca gcaggatatg aattttacca aaaggataca
    25741 aaagtgtgga gaaatgatgg ggatcactgt actggaccac ttgatcatcg gacgcaaacg
    25801 atattttagt ttgagagaag aaggaatgat ggaagagaag taagttgctt tcttgcaaaa
    25861 aaaatgaagc aagtgtacaa ttatattgta attttgtttg ggaatagaag ttgaaacatc
    25921 tgttgcacct ctatcgtaca cgagcaatgt tacaatttaa attagtgctt ttggcacgag
    25981 gaggatatta ttatggaaaa cggaacagta aaatggttca acgctgaaaa aggatttgga
    26041 tttatctcac gtgaagatgg aagcgacgta ttcgttcact tctcagctat ccaaggtgac
    26101 ggattcaaaa cattagaaga aggtcaagca gtgactttcg acgttgaaga atcagaccgc
    26161 ggcccacaag ctgcaaacgt tgtaaaagct taattttatc tgtaaatcaa attgatggta
    26221 gacatcaatt tttacgagag aacaaagcga gtaacaaaaa tacctgccac aagagacaat
    26281 ttctcttgtg gcaggtattt ttttgtatga aaaagtaaat aaatttaaga ggctatgaaa
    26341 gagccacctt gtatcaagga ataagaacaa acaaaaatgg cttctcatgt ttttcatttg
    26401 ttttctctta tattcaggac aaggtgctga tttcataacc tcttatttat tcttcatctg
    26461 gtgtcattac cattggcaaa atcattggat gtctttctgt acgttcgaat aagaaaggct
    26521 gaagcgcgcc agtcatcgct tctttcaatt tatattctga acagtcaggt ccattcaaac
    26581 attctcttaa agcgtggaat aagattcgct gagcttcatt gatcatttct cctgattctc
    26641 gcatgtagac aaatccacga gagagaatat ctggaccagc caagatttct tttttcttga
    26701 tatccactgt cgcgacagct aatacaagac cttcttctga aagcaggtga cggtcacgta
    26761 aaacgacatt accaatatca ccgatcccgc taccatcgat atacacatct ccagccacaa
    26821 aatgcccagc aggacgcgca gaatcgctag ttaacgctaa aacatcgcca ttttccatga
    26881 tgaagcagtt ttctgcagga acacctacat cttgtgctag agaagcatgt actttcaaca
    26941 tgcggaattc tccgtggaca ggaataaaat attttggctt catcaaacga agcattaatt
    27001 tttgttcttc ttgtccaccg tgaccggaag tatggatgtt attgattttt ccgtgaatca
    27061 cttctgcacc agcttcagac agcaaattga tcaaatgatt gacacttgtt gtattaccag
    27121 gaataggtga acttgaaaag atcactgtat cgtctggctg gatttggatt tgacggtgag
    27181 taccattcgc gatgcggctc aacgcggcca tcgcttctcc ttgagaacct gtacatagga
    27241 tcatcgtttc attggcaggc aagcgattga tttcgtgtgc atctacaaat gtgccgtctg
    27301 gaactttgat atatcctaaa cgttgaccgt tgacgatggc attttccatg cttcgtccga
    27361 aaacagcgat tttacgacca gttgccacag cggcatctgc tgcttgctgc aaacggaaga
    27421 tgttagaagc aaagctggca aaaatgatac gtccttcgat ttttgaaaag atcttcaaaa
    27481 tagaatgccc aatcgttttt tctgatttag taaatgttgg aatctcagca tttgtactat
    27541 cagataaaag gcacaagact ccttcttccc cgatacgagc cattttatgc aagtttgctg
    27601 gttcgccaac aggagtgaaa tcgaatttga agtctccagt cgctacgata tttccagaag
    27661 gtgttttcac cacgatccct aatgcatctg ggatactatg ggtcgttctg aaaaagctga
    27721 cagaagtctt tctaaaacgg atgatcgtat cttcattgat ctcatggagc tctgcatctc
    27781 gaagcaagcc atgctcatct aatttgtttg tgatcaacgc taatgcgaga ggaccagcat
    27841 agataggaat atttgcttgt ttcagcaagt agggtattcc gccgatgtgg tcttcgtgcc
    27901 cgtgagtaat caccaaggct tttactttgt gtaagttttg tatgatgtag ctgtagtcgg
    27961 gaatgacata gtcaattcca agtaaatcgt cttccgggaa tttgatcccg gcatcaatca
    28021 aaatgatttc atcttggaat tgtaccccat acgtattttt cccgatttca cctaatccac
    28081 caatagcaaa gacgcctgtc tcgttatttt tgatggaaag tttcatatta aaactccact
    28141 aatgagaaat cagcattttc tttttcgtac tctagatgac ttccttctag aagttgaacg
    28201 aattcaatat tgtatggggt attttcttcc acacgttggc gagcgtctac atcgcttttt
    28261 gcctcaatgt aaagagattt tgtttcttcc cgtttagggt tgcgtacttt tgtttcttga
    28321 taataaactt tatagatcat tgttttgtct cctttatctg cgtaaagcag tgatttagct
    28381 agttgatttt tttagacaac acaaaacgag gggacaagcc acctggtttt tcatatgctt
    28441 tgactagaat actggattcg agtcaaagag acaagcctcg tttttgctta tctgttcgga
    28501 tattttttca accgtacctt ctgtataaaa aatatacaac aattaattgt tattctagca
    28561 taaaacagca aataagtata gaaatcaact agattgaaag aaaaaatcaa tgtttatcat
    28621 cccgctataa tcataatgac ttttttatga gagaagtgtt ctattaatag gctcgttgtg
    28681 ctatcatttt tagtaatgaa aatgtaacaa aagattaaaa ggagatgaga aagtgaaact
    28741 aatgtggcat tataccatgc gttataagaa attcttgttt ttcaatttca tatgcgtatt
    28801 tggttttatt ttgattgaat taggacttcc tacgatttta gcacgtatga tcgatgtagg
    28861 aatccgcaat gatgattttg attatgtgaa gcaacaaggg atatttatga tcgttattac
    28921 tgtaatcggt attattttga atatcttgtt aggttatttt ggttcgagga ttacgactaa
    28981 tatcgtagca gatattcgag atgatttgtt cgaaaagatc caaagttatt cgcatcagga
    29041 atacgaaaat ctaggcgtat cttcattgat cactcgtaca acaaacgatg cttatcagat
    29101 catgttgttc ctgcaaaata ttttacgtat cggttttatg gcaccaatga tgttcattgt
    29161 cagtttgtac atggtcatgc gtacaagtgt gacactcagt ttgtatgtag taggtgcatt
    29221 acctttacta ttattggcag tcgtagcgat tgctaaggtg tctgaaccgt tatctaaaaa
    29281 gcaacaaaaa aacttagata aaatcaacag tatcttgcgg gaaaatcttt caggattgcg
    29341 tgtgattcgt gcattcgtca atgaaaagtt tgaagaaaaa cgttttgctg tagtcaatga
    29401 gaattatgct tctagttcaa aaagcttatt ccgtttgatg gctgttgcac aaccgggttt
    29461 ttatttctta tttaatatcg taatggtatt gatcatctgg agcggggcag tgcaaatcga
    29521 tgcaggcgag cttcgtgtag gagatttgat tgcgtttatc gagtatatat tccatgcctt
    29581 gttctcattc atgttatttg ctagtgtgtt catgatgtat ccaagagcag ctgtttcagc
    29641 tggacgtatc caagaagccc tagatgccaa accttcgatt gtagaagatc caaatggtat
    29701 atctgataca aaaactaaag ggtatatcga atttcaaaat gtaacatttg cttatccagg
    29761 ccattcgcaa gagccagtga tacgaaatgt cagttttaca gcctctccag gagagaccgt
    29821 tgcttttatt ggaagtaccg gtagcggaaa atctacgttg atccaattga tccctcgctt
    29881 ttatgatgtg agtgaaggaa agatcttgat cgatggagta gacgtgagag attatcggct
    29941 aagcaagttg cgagataaga ttgggtttat tccgcaaaaa gcgctgctat ttactggaac
    30001 gattgcagat aatttgcgat acgggaaaga agatgctacg caagaagaaa tggaacgtgc
    30061 agctgagatt gctcaagctt cagaattcat ttctcgaaaa ccagatggat ttaatgaaca
    30121 tttatcagaa ggtggttcca atttttcagg tggacaaaaa caacgattgg caatagccag
    30181 agccatcata cgccgtccgg aaatctatat ttttgatgac agcttttctg cattagatta
    30241 tcaaacggat gcgaagttac gtgctcgtct gaaaaaagaa acgacagaat ctactgtatt
    30301 gatcgtagca caacgtgtgg gaacgatcat gcatgcagat aagatcatcg tattaaatga
    30361 aggagaagtc gtcggtatgg ggacgcatcg ggagttgttg gaaaaatgcc cgatttatta
    30421 tgatattgcc gcttctcaat tatcaaagga ggaattagca tgaaaaaaac cttttcttcc
    30481 ttaaaacgtt tagctcgata tatcagaccg tatcgtgaga cttttctttt agtgatccta
    30541 tttacagttc tgacagtggc tttcaacgtg gctttgccat atgttacagg acttcctact
    30601 acagaaatca gccgtaatat tgccaatggc gaatcaatca atttttctta tatattcaaa
    30661 tgtctcattt ggatcacagt tgttggaata gggtattgta tttcgcaatt actttctggt
    30721 gttttgatga caaatgtcgt tcaaagtgcc atgcatgatt tgagaagaga catagaagaa
    30781 aaaatcaatc gtcttccggt ttcatatttc gataaaaacc agcaaggaaa tattttgtct
    30841 cgtgtgacga atgatgtaga tgcagtcagc ggtgctttac aacaagcttt tattggtatc
    30901 gtaaatgcga tattgggaat tacgttggct gcagcaatga tgttttatat ccagccgatg
    30961 atggcgctga tctctatgat catgattcca acatcgatct ggatctcaaa acgagtgatc
    31021 aatgcttcgc agaaatattt tcaaagcatg cagaattctc ttggtgagct aaacggctac
    31081 gtccaagaaa atatgacagg ttttagtgta ttgaaagtat atggacgcga aaaagaaaca
    31141 ttggaaggat tcaaaaaagt taatcacagc ttgaaatact ttgggtttcg tgcagcattc
    31201 atttctggct tgatgatgcc tctagttcaa ttaactgcct atgctactta tattggtatg
    31261 gctgttctag gaagctttta tgttattact ggggcgatcg ttgtcggtca actacaagca
    31321 ttcatccaat atatttggca aatcagtcag ccaatgggga acgtgacaca attgtcttca
    31381 gttttgcaaa gtgcatcagc ggcaacgaaa cgtgtattcg agattttaga tgagccagaa
    31441 gaaaaagaaa acaaagtaga tgcttcactg ccagaaacga ttttaggaga tgtgacattc
    31501 gatcatgtcg attttgcata tgatccgaag aaaccattaa tccaagattt gaattttgaa
    31561 gtgaaagctg gtcaaaccgt agctgtggtc ggtccgaccg gtgccggcaa gacgacattg
    31621 atcaatctat tgatgcgttt ttatgacgta gacaaaggag caataaggat tgatggaatc
    31681 gacaccaaat caatgagtcg cagtgatgtg cgttcgctat ttggtatggt cttgcaagat
    31741 gcatggcttt atcaaggaac gattgcagat aatatccgct tcggaaagct tgatgcgaca
    31801 gattatgaag tagttgatgc tgcgaaaaca gcaaacgtag atcattttat ccgaacgatg
    31861 ccagatggat atgatatggc catcaattct gagggagaca atgtgtcttt aggacaaaaa
    31921 cagttgctga cgattgcccg agcagtggtc tccgatccta aaatcttgat tttagatgaa
    31981 gcaacaagtt cagtagacac ccgtttagaa gcgttgattc aaaaagcaat ggatcgcgtg
    32041 atggagggac ggacaagttt cgtcatcgct caccgtttat cgacaatccg tgatgcagat
    32101 ctgattctag tgatggatca aggtcagatc attgaaaaag gaacgcatga gagtttattg
    32161 gcacaaggtg gattttatga aaaactgtat aacagccaat ttgctgagga agcagaataa
    32221 gtcatataag aataaaacag tttaaaactt attttttgtt ctctattctt gataatggta
    32281 aagtaaagtt atcaagaaaa gtgaggacac ggagatggaa cagtttttaa atgaaaaaat
    32341 tactgaagcc aaaagagaag ttgatcgttt aacgcacgtc tgtcagacga agccttttga
    32401 ttcagctgat ttttttgaaa atgagtttca aatcgaacgc ttggcagcac aaatagaagt
    32461 gtacgaatta attttaaaaa aagtaataga agaaaatata gatgtacaat aaaaacaggt
    32521 aaaacgtcag ttaccagata ctgtaaatag acatctggcc tgagcaggtt ttacctgttt
    32581 tttttcttat gaaataaata tatgctatga tacctttatt caataggatt agtttctaca
    32641 aaaaatgaaa ggagaaaaat aaatatggaa cggtttatag catcaaatgc cacagtgatt
    32701 ggtgatgtga cattgtcaga agatgtgacg atctggtatc aagctgtttt acgcggggac
    32761 agtaattgga taaaaatagg acaaagaacg aatatccaag atggtacaat catccatgtc
    32821 gatcatgatg cacctgtaga tattgcggag aatgtgacgg taggacatca gtgcatgcta
    32881 catggctgta caatcgaaaa aggggcgctg atcgggatgg ggaccacgat attgaatcat
    32941 gcggttattg gtgagaatag tttgattggt gctggatcat tagtaacaga aggaaaagtt
    33001 attcctccta atgtcttggc ttttggtcgc ccagctaaag tgatccgtcc tttgacaaaa
    33061 gaagaaatac aaaaaaacaa agaaaacatc cagcattatg tcgagatagg tcggatgcat
    33121 gcgcaaggaa aattcaagga gcaaagatag gcagtagcat ccataaaata aaaaaggatg
    33181 gtcagtttga gttgaccatc cttttttatt tggtttagat aagagagctt aaaaaagctt
    33241 cctttgaaac agattaattt aatgaagaac tgttttccat gacttcatcg atcaatccat
    33301 atgcttttgc ttgttcagct gtcatatagt tgtcacgatc tgtatctttt tcaatcactt
    33361 caagcggttg tccagtgcgt tcagctaaaa ttttgtttaa gcgttcgcgt gtttgcaaaa
    33421 tatggcgtgc cgcaatttcg atttcagttg cttgcccttg agcgccgcca agaggttgat
    33481 ggatcatgat ctcagcattt ggtaaagcaa atcgtttacc ttttgtacca gctgtcagca
    33541 agaagcttcc cattgaagca gccataccca taacaattgt ttggacatct gctttgacaa
    33601 agttcattgt gtcgtaaata gccatacctg ctgtaactga accaccagga gaattgatat
    33661 aaagatagat atctttttca gaatcttgag cgtctaagaa aagcaattgg gcgatgatcg
    33721 aattagcaag atcatctgtt acttggccgc ttaacatgat gatacggtct ttcaataaac
    33781 gtgagtagat atcgtaagct ctttcacctc gtgaagattg ttcaatgact gtaggaatta
    33841 aattcataat agagttcctc cttgatttcg tttagaaata gtgtaccata atttagtttc
    33901 taatacctaa aatgtagtat acatttttgg tcaaaaaagg tcaaataaaa aacttaggca
    33961 aattagtcgc ataaacccgc tttatggtat gataagcaga gaaacaaaac aaggaggaac
    34021 aacagattga tagttggtat tactggcctt atcggagtgc tgacaggtgc tgtagttgtc
    34081 tatatcggca tgatactgca aatgcgtcac ttggaaaata aagaacagcg tagacgtgaa
    34141 ttggaattga aagtcaaaga aatcgagacg ctgaatcaat taaataaaaa gatcaacgag
    34201 atcttgcaaa aacggacaac gctgatggaa gaatatgtca gctttgatgc atttgatgat
    34261 tgttatatca cgattgacga ttttgcgtat ctccaatcat tttcagcgca aaatagtttt
    34321 tatctgccaa attatatttt agacgagcta tttaagaata tctcccatcg aaaagtgatt
    34381 ttatctccag atgaaacaat gaagattggt ggatatgctt ataaaggcgg acgcgtgatt
    34441 ttagagaatt ttacggatag tctgactgaa atgatcaatg aaaaaaaagc tcagttgaag
    34501 actatgacgc gccagccgat tggtttcttc tcgaaagatg atgtttggag ctgatatttt
    34561 gaagcattgc cgttgcacat gagagcgtgg taatgctttc tttttttctt aatgatacaa
    34621 tagaactgaa cggagtgaag aaagatgtta caaatctatg cccatcgcgg aagcagcggc
    34681 acccacccag aaaatacgct tcctgcattt gcagaagctg tacgagtagg tgcagatggt
    34741 atcgaactag atgtccatct aagcaaagat ggttatttga tcgttatgca cgacgaagaa
    34801 gtggaccgga cgactaacgg taaagggctg attcgagaaa aaacattgga agaactcaaa
    34861 aaattgaatg cagggggcaa attcagcaaa gaatttccag cagctaaagt tcctgaattg
    34921 agagaagtgt taggactgct cctccaaaaa aattaccgag ggatgctaat gattgaaatt
    34981 aaaactgatc agtatgaata tcccggtatc gaagaaaaat tagccgaaac attaaaaagt
    35041 agagattggc cttttcaaca tgcatattgc agctttcatt tggattcttt ggaaaaaatc
    35101 gcattactgg aaccggagat acagctggac ttattgatga gtacttctga taaaaagatc
    35161 caactagcag cagaaacccc ttatatagaa ggagtacatc caaaaaatac ttggttgttc
    35221 gaacatccag tccaggcagc tgattttcca aaagcaattc gtgcatggac gattaacgat
    35281 gaagaagaaa tggccaaatg tcaagaactc aaaatagatg gaataataac tgattttccg
    35341 gagaaagctc ttcggtggaa acaatcaacg aaaagtaggt aaaatatgga aaaagtatta
    35401 gtgatcgtcg gtccgacagc agtcggtaaa acagcgctaa gtgtggaact cgcacaacta
    35461 tttggaggag agatcatcag tggtgattca ttgcaaatct ataaaaatct ggatatcggt
    35521 acagcaaaaa tcaaagaaga agagatgaat aatgttccgc atcatttgat tgatgtgatc
    35581 gaacctactc aacgttactc agccgctgat tttcagcatt caggtcgaca attgattacc
    35641 gagattgcga accgagggca cctacctatc atagctggtg gtacgggtct ctatatccaa
    35701 tcccttctat atgattatca gttaggagcg acagaagaag cgaatcctga gattagaaaa
    35761 aagtatgaaa cgttagctga aacaattggt tcccaagcat tatggaatca gttgaaagaa
    35821 aaagacccgg cagctgctga gaaaatccac tggaacaatc aacgcaaagt gataagagct
    35881 ctggaagttt tagagacgac aggacatagt atcacggcac ctaaagaaga gccaaagcaa
    35941 ttgtatgatt acctcatgat tggattagat acgaagcgta gtattcttta tgaacgaatc
    36001 aatcaaagag tcgatttgat gctagcagaa ggtttagtag cagaagcgcg taaggtgtat
    36061 gagcttggag aagtccaggc cagtcaaggt attggatata aagaattttt cccttatttt
    36121 gaagggaaag aaacattgga agaagcaaca gaacaagtca aactacattc gagaagatac
    36181 gcgaaacgtc agttgacgtg gtttcgtaac cgtttggatg cacagtggtt tgatcttata
    36241 agtgagccaa atcaaaaaag tcagattgag caaacaatca aaaaatggct ggaggcggaa
    36301 taaaatggaa gaaaaagtga tcatcgtagg ggtagaaacc gaagcaaacc agcgttactt
    36361 ttcagagtca atggaagagc tagttcagct gacgaatacc gcatcaggag aagtcgtatt
    36421 cacaattaca caaaagagac cacaagtgga tcggcaaacg attatcggta aagggaaatt
    36481 acaagaatta gttcaacaag cggatgcgca tgaagcggat ttgatcatct ttaatcatga
    36541 gctaactccg agacagagcc aactgattac cgatgctgtt ggtcttccgg taattgatcg
    36601 cgtgcagctt atcttagata tttttgcgat gcgtgctcgt tcgaaagaag ggaagctgca
    36661 ggttgaactt gctcagttag aatacctgct gcctcgttta gtagggcaag ggaaaacatt
    36721 atctcgtctt ggcggtggga tcggaacaag agggccaggt gaaacaaagt tagaaacaga
    36781 ccgccgacat atacggaata aaatttttgc tgttaagaaa gaattgaaag aagtagaagc
    36841 tcatcgagag cgcaatcgcc aaaaaagaaa aaatagcgag atttttcaga ttggacttat
    36901 tggctacacg aatgccggga agtcaactat cttgaatcta ttgacacaag cggatactta
    36961 ttcgaaagat caattgtttg caacgttaga tccattgact aagagatggc gttttgctga
    37021 aggatttgag attactgtaa cggataccgt cgggttcatc caagacctgc ctacgcaact
    37081 gatcgatgct ttccattcta cattagaaga aagtcaaaat atggatcttt tgctacatgt
    37141 agtcgatgct agttctccag atcgtatttt acaggagcag actgtcttaa aattgatgga
    37201 tgaactgaat atgaaagaaa tgccgatact gactgtttac aataaggcag accagatcga
    37261 cccagcgatg ttcacgccta cgctattccc aaatgtattg atttctactc agacgaaaga
    37321 aggtaagatg gcgttgatcg aagcaatcaa acgtcagctg atggaactaa tgacccctta
    37381 tacactttct gtgccaagca acgaaggaca gaaattaagc gagttgagaa gacaaacaat
    37441 ggttttggac gaagcgtaca tggaagaaac aaacgaatat gaagttaaag ggtttgcaaa
    37501 aaccaattca aaatggttaa gaaaaaacca ataaaatttt atttttttaa
//
LOCUS       DAZULO010000015        35902 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000015 DAZULO010000000
VERSION     DAZULO010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.015"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(176..2521)
                     /locus_tag="ACG8OA_000809"
     CDS             complement(176..2521)
                     /locus_tag="ACG8OA_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFS4975644.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            2800..3702
                     /locus_tag="ACG8OA_000810"
     CDS             2800..3702
                     /locus_tag="ACG8OA_000810"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFS4975645.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            3915..5690
                     /locus_tag="ACG8OA_000811"
     CDS             3915..5690
                     /locus_tag="ACG8OA_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFS4975646.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5756..7342)
                     /locus_tag="ACG8OA_000812"
     CDS             complement(5756..7342)
                     /locus_tag="ACG8OA_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFS4975647.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7593..7817
                     /locus_tag="ACG8OA_000813"
     CDS             7593..7817
                     /locus_tag="ACG8OA_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFS4975648.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7959..9710
                     /locus_tag="ACG8OA_000814"
     CDS             7959..9710
                     /locus_tag="ACG8OA_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFS4975649.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            9710..11494
                     /locus_tag="ACG8OA_000815"
     CDS             9710..11494
                     /locus_tag="ACG8OA_000815"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4975650.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            11820..12671
                     /gene="licT"
                     /locus_tag="ACG8OA_000816"
     CDS             11820..12671
                     /gene="licT"
                     /locus_tag="ACG8OA_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFS4975651.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            12764..14650
                     /locus_tag="ACG8OA_000817"
                     /pseudo
     CDS             12764..14650
                     /locus_tag="ACG8OA_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
     gene            complement(14690..15592)
                     /locus_tag="ACG8OA_000818"
     CDS             complement(14690..15592)
                     /locus_tag="ACG8OA_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4975652.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            15823..17226
                     /locus_tag="ACG8OA_000819"
     CDS             15823..17226
                     /locus_tag="ACG8OA_000819"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS4975653.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            17226..18659
                     /locus_tag="ACG8OA_000820"
     CDS             17226..18659
                     /locus_tag="ACG8OA_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS4975654.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            18804..19730
                     /gene="trxB"
                     /locus_tag="ACG8OA_000821"
     CDS             18804..19730
                     /gene="trxB"
                     /locus_tag="ACG8OA_000821"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFS4975655.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            19944..21671
                     /locus_tag="ACG8OA_000822"
     CDS             19944..21671
                     /locus_tag="ACG8OA_000822"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFS4975656.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            21686..22036
                     /locus_tag="ACG8OA_000823"
     CDS             21686..22036
                     /locus_tag="ACG8OA_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFS4975657.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(22128..22988)
                     /locus_tag="ACG8OA_000824"
     CDS             complement(22128..22988)
                     /locus_tag="ACG8OA_000824"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFS4975658.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            23080..23508
                     /locus_tag="ACG8OA_000825"
     CDS             23080..23508
                     /locus_tag="ACG8OA_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS4975659.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            23670..23846
                     /gene="rpsU"
                     /locus_tag="ACG8OA_000826"
     CDS             23670..23846
                     /gene="rpsU"
                     /locus_tag="ACG8OA_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFS4975660.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            23873..24319
                     /locus_tag="ACG8OA_000827"
     CDS             23873..24319
                     /locus_tag="ACG8OA_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFS4975661.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(24473..25852)
                     /gene="brnQ"
                     /locus_tag="ACG8OA_000828"
     CDS             complement(24473..25852)
                     /gene="brnQ"
                     /locus_tag="ACG8OA_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS4975662.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            26558..27529
                     /locus_tag="ACG8OA_000829"
     CDS             26558..27529
                     /locus_tag="ACG8OA_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFS4975663.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            27553..29745
                     /locus_tag="ACG8OA_000830"
     CDS             27553..29745
                     /locus_tag="ACG8OA_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFS4975664.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            29762..30238
                     /gene="ybeY"
                     /locus_tag="ACG8OA_000831"
     CDS             29762..30238
                     /gene="ybeY"
                     /locus_tag="ACG8OA_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFS4975665.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            30222..30617
                     /locus_tag="ACG8OA_000832"
     CDS             30222..30617
                     /locus_tag="ACG8OA_000832"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4975666.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            30632..31531
                     /gene="era"
                     /locus_tag="ACG8OA_000833"
     CDS             30632..31531
                     /gene="era"
                     /locus_tag="ACG8OA_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFS4975667.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            31674..32486
                     /gene="recO"
                     /locus_tag="ACG8OA_000834"
     CDS             31674..32486
                     /gene="recO"
                     /locus_tag="ACG8OA_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFS4975668.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            32870..33787
                     /gene="glyQ"
                     /locus_tag="ACG8OA_000835"
     CDS             32870..33787
                     /gene="glyQ"
                     /locus_tag="ACG8OA_000835"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFS4975669.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            33789..35864
                     /gene="glyS"
                     /locus_tag="ACG8OA_000836"
     CDS             33789..35864
                     /gene="glyS"
                     /locus_tag="ACG8OA_000836"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFS4975670.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        11639 a         6420 c         7602 g        10241 t
ORIGIN      
        1 tttgttataa tccttttttt gtgtcttaaa aaagagagaa agtggttgaa attacattta
       61 tcgtatataa aacaatccgt gtatagatgc tgcattttgt tcaccaccat caaaaaaata
      121 agggactgtg acaatactat tgtcacaatc ccttattact gtgtattcta ttaaatcaat
      181 catctttctc atctgattct ttagttgctt gattcgctct tgcataagct gaagcggtgt
      241 tccagtaatc tgtatagttt tcatcggtac cgccaacgcc aaatttaaag gcgcttttct
      301 tcggtccatt tttcgcccat agcgattcaa ctgtttttcc tggtgtttgg atggattttt
      361 tattgacagt aacttttcca ggtgtttgac cagtaaatcc agatactttt ttcttcatca
      421 catcagatga taattcgaat ttctcatcag tgccaaaaat gtcaggtttt gcttggtaag
      481 cttgattgat cagattggca agataattag ccgaacgtat ccttgctttg ctgtccattc
      541 cagtattgtc atcgtgccct gcccagctac tgatggtaat agacggagta gagacaatta
      601 gccatgaatc tcggtattca ttggtagaac cagtttttcc gacccaatct gcttttccta
      661 gatttccgtt taagcttgag atggcatctt tgaatggtgt agtgattttt gcattgatca
      721 cactacgcat catgtcattc atgatcgatg ccgttgcttc cgaatagatc cggacagagt
      781 tgctttcgtg tttataaatt acatttcctg cgttatctgt gatggaatcg atgatatagc
      841 cttcttcata gacgccgccg tttgctaaag cctgaaatcc gttggtctgt tgaagcgtcg
      901 tgacatctac tgtacctaaa ggagcagatt caacgcccca attgtcattt gccgggtagt
      961 tcatctttgc caagtagtgc tcgtaagcat attgtttcga tccaccggaa ttcaacacat
     1021 cttgatataa gtgataagcc gggatattgt tggaccattc tagtgactca cggacggttt
     1081 ggaaggtgtt cgatccttcg ttagtagcat tgacgatttt ctcgccagca ttttctcctt
     1141 cttgccaagt tgttgcataa tctgatacac gggattctga tccgataagc cccatgtcga
     1201 ttgcaggtcc ataaacaaga actggtttga ttgatgagcc agcttgacgt tctgtatcga
     1261 aggcatggtt attttgattc tgactgtaat tccgtccgcc aacgaatccg tagatgcggc
     1321 cagttctgtt atccatcaat acatttccgg tttctacttg actgccagca ccatcatcta
     1381 acatgtatcc ataattttca acgccatttt gcaatgcagc atagacatct ttatcgattg
     1441 tcgaatggat tgtatatcct ttattttgga tcgtttgctg tgccttttga taataagcgt
     1501 cactgaccga tgaatcagac atatcagcgt tgtctttttc agccaattgt tgtgcaatga
     1561 ttcttgttgc ttcattcatc acagtataat acaagaattc tctgtcagtc tgttctgcga
     1621 tttgttgtgg tagaaagtct tttgtaaggt cataggcttt tgcttcttcg tattgttctt
     1681 tcgtaatttg gccttcacga tacatactga ataaaacaaa atcttttctg gcaaggccgg
     1741 cagaaagatc ttcttttagc gcgcctgtgt tcgtgtatgg actatacgtg atcgggcttt
     1801 ggggtaatcc agcgatataa gcagcttgcg gcagcgtcac atcttttgca tctacaccaa
     1861 agattccttt tgcagcttcc tggactccag caatgttttg tcccttatta ttacgaccaa
     1921 atggtgagac attcaaatac gtagcaatga tctcatcttt tgaaaagtat ttttctactt
     1981 gtgcagaaag aatgatctcg tttgctttac gtttgaatgt tgtttcatct gtaaggatct
     2041 gctgtttgac taattgctgt gtcaaagttg atccaccaga agagccaata ccagtagctt
     2101 cagatactaa tgctcgtaaa acagctttag gaacgtaacc ttggtgttta tcgaagtatt
     2161 catcctctgt gctgataatt gcagttttca ataaaggtga aatcttatca gatgaaatag
     2221 tcgtgcgcat cagatctgtc tggatttttg atatctctgt attatctgca taaacaatct
     2281 ttgacgtttc tgtaatgttt cctagttctg tctgcagtgc ttttttagtc ggcaattgtg
     2341 tgtcttcgac gagataggca aaatagccag cgcctattcc aatacctaga gctgcgaaca
     2401 tcaaaagaag aacagtaata aaaacagtca atgattgaaa aactctaaaa ataatcttag
     2461 gaacaaacca tttttctttt tttcttctat tctgtttttt tcgagtattt ttgcgtggca
     2521 aaagggtcac ctcaactaat ggttttattt cgctaattat agcaaataat agaacaaaaa
     2581 aacagacaag gctgtttttg gcaggaattt cttttttaaa agagttaaaa cgtgagaaaa
     2641 tgatctttat tataaaagag ttcttaagct tttctttaga gaaaatttca gttactatgg
     2701 agaacagggc aaagaaaaat caatcatttt ctttgtatat catcttacca atatatagga
     2761 attgttagta acaattgcct ttcaaggagg aatccatcca tgttattagg ctcacacgtc
     2821 agcatgagcg gaaagaaaat gctgttagga gcagcagaag aagcagccag ttatggatct
     2881 tcgactttta tgatctatac aggcgcccct caaaacacta gaagaaaagc catcgaagag
     2941 atgaatattg atgccggaaa agcgtacatg gcagaaaaag gtctaacaaa tatcgtcgtt
     3001 catgcccctt atatcattaa tttggggaat acgatcaaac cagaaatgtt tccttttgcc
     3061 gtccaatttc tgagagaaga gattgttcgt gcggaagctt taggcgctac tcaaattaca
     3121 cttcacccag gtgctcatgt aggggcagga acagaagccg gaattgctag aattattgaa
     3181 ggcttgaatg aagttttgac aaaagaacag acggcacaaa tcgctttgga gacaatggca
     3241 ggtaaaggta cggaaatcgg tcgaacattt gaggaattag cgcaaatcat cgatggagta
     3301 aaattgaatg aaaaattatc cgttactttt gacacctgtc atacgaatga tgccggttat
     3361 aatattaaag aagacttttc aggtgtaatg gaagagtttg ataagatcat cgggttagat
     3421 cggcttaaag tactccatat caatgattct aaaaatccgc aaggttcgca taaagaccgg
     3481 cacgccaata ttggttttgg aacaatcgga ttcgacacgc tgaataagat cgtccatgat
     3541 ccaatgtttg acaatatttc gaaaatcttg gaaactccct atgtaggcaa agataaaaag
     3601 aaagcttacg caccatatgg aaaagaaatc gccatgttca aagcgcaaac attcgatcca
     3661 gaagtgtttc ctgagttatt agcagaagaa gtgatactct aagattttct atctgacagc
     3721 agcaaaaaag gaccaggcac attgccttgt ccttttttgt ttaagaaaca aaggagtact
     3781 ttttgcagtc tttttcatca tgcatcttct cccccctctt tttaaattta gacaaacaag
     3841 gtatactggt agaaaagtaa aaaaacgaaa agtttcacaa gcaagtggca aaacgtgatt
     3901 ggaaggacga aaagatgctg aacactttaa aacccttgat caaccaacta aaaaagaaac
     3961 atacatactt tattcttggc atagccctat tatttacaat tattcgtttg tatttatata
     4021 gtaaagcaat ctatggaaca gacatgtcgg caacctatga tgaccagctg ctgattcgct
     4081 atgctcaaaa tattttggac ggtaaatggc taggggagta tacgactaca acactttcca
     4141 aaggaatctc ctacagctta tttttagtac ttgcacataa actgcatctt tcttatgcag
     4201 ttttgttcgg catgttcagt atttttgcca gtatattggt tgcactcgcg tttaaaccga
     4261 ttgtgaagaa caagtatctt cttttaagta tctattttta ttttttgttt tctccgatta
     4321 gttttacaca tgaatattcc actcgaacat atcgtaatgc cctcgtgatt ccggctgttg
     4381 agatcattat cgcttgttta ttagctattt attaccgcaa atacggttct agcagaaaat
     4441 tgctgccatg gtttttaggg ctttctgtcg cttttccttt cttttggttc attcgcgaag
     4501 attctatctg gcttcttcct tttactgtag tagctttggg tatcagtttc gtccagatct
     4561 tgctagggca agacttttct ttcaaagaag taaagaagtt aaaaaaacga ctctttcttc
     4621 tctcgaaacg ggaatggctg aaactttcat tgtttttact tcctatagtt ggcttttcca
     4681 tcgtctatca atggatcgaa tataaaaatg agacaacata cggtattgca accatcaatg
     4741 accgttccag tggagaattt ggacgtttga tgaaacaact gatccgaata gatgatgaaa
     4801 cgaacttgaa taaagaaaac agtgagatat gggtctcaca tgctgcacta aataaagcat
     4861 tagccgcatc tcctacattc catactgttt ctacccgtat tgacgaatta tatacgactc
     4921 acgcgtggac tcaaggtgga aaagtaaaag aattacccgg agatatcatc ttttgggcat
     4981 tgcgagatgt ggtaagcgaa tctggttatt accaaaacaa tgcaaaagaa accaatgatt
     5041 tttggaaaaa agtcaaccaa gaattagaag aagcctatca caagggaaca atcaaaaaga
     5101 aaaaagagtt ctatctgatg ggatcaggag atggaaagac gatgagtgat ctgccaatcg
     5161 tattcgattt ccttcaatca ggatacgctt atgcatggcg atataaagat ttccatcaag
     5221 gaggaaatta tagtttcgga acgcaagaac aagtcaaaca ggctcaagaa ttattaaacc
     5281 ttccgctaat ggagaattgg atcgatagta atgaaccgag aacacctaat ttccaattaa
     5341 caaaatcagc aaaagtcgcg aatgtcatca taaaaattta ccaagttctg gctattcccg
     5401 tgctgattgc ttcaatagca gggatattac ttatgatact aggaaccgta tttgcaaaag
     5461 ctgatcgtgc ctattatagt tcctctttga ttgtggtcac cggattaata ctgacacaat
     5521 tggtttttct tttcggtgtc tcatggtttt gttcttatgc gccagaaaaa aaggactatt
     5581 ttttaagtgt ctacactggt gcaggcgtgc cattaacgca attcatcgta gtaacaagca
     5641 tgctagtttt cagtaaatta aatctgaaaa aagctttatc ttctaaataa aaagaccctt
     5701 ggtagctaga tttttaacct aactatcaag ggtctttgtg tggaaaaatc agtttttatt
     5761 cgatttcttc taccgtttcc tgcaaaagct gttcatattt tttgtttcct ttatataaat
     5821 caccaatcgt ccatgccaac aggagaagga caagaacaat gattacataa gcgatgtgat
     5881 cagccaatgc tagttgagcg gaagtggctt gctcgccgaa gaaactttcg atctgtcctg
     5941 gtaatcctcg taagttcaag taagttagac taatcacaga gaaccagcca agaagtttta
     6001 cgatgaatga atttttaaat cggctaccca tctccgcttg gctgttggtc atcatcagta
     6061 aaggaatcat cgaaaacggc aacgcaaaag ccaagaacac ctgggattca ttcattaagt
     6121 tattcaaagc ggcatgttct tcgatcgttc cttttgtact tgtaaacaaa acacaaatca
     6181 atacaggaat cacagaaagc aatcgtgtga ccagtcttct tagccaaatc gggatacgca
     6241 tatggatgaa tccttccatg atgacttgac cggtcaacgt accagtaata gtagagttct
     6301 ggccagaagc aagaagtgct acagcaaata aagtggacaa gataccagat tttgctacag
     6361 caatcaaaat accattgctc atagctgatg gatcagataa agcttcatac agaccaaaaa
     6421 aggaaggatc ttggacagaa ccggttttga agacagcgac acccatgatc aatagcagtg
     6481 cattgactag aaaagccatt gacaactgga tattagaatc ccaagtagaa aaacgtacag
     6541 cttgtgctac atcttcttcg ttttggtgat cgatcttccg agtttgtgaa actgctgaat
     6601 gaagatataa gttatgcggc atgactgttg caccaataat ccctaaagct ccagtcagcg
     6661 gagtttcgcc gccgatgacc ggtgagtctg agaatgtatg actgttaggg ataaagccag
     6721 caaataaact gtgccaatct gggttagaca agccaacttg atagacaaaa acgaataaaa
     6781 tgacgaaaat taagcagaca accagtgctt cgatcttacg aaacccgact tttgtcagta
     6841 gtaagagaac gaaaacatct aatacagtaa taaagacggc aatcaccaat ggaatatcaa
     6901 aaagtaagta gagggcgatt gctgcaccaa tgacttctgc aatatctgtt gccataatgg
     6961 ctaattcagt caagatccat aaaatgacac ctaatgtctt acttgtgcgt gctcggattg
     7021 cctgtgccaa atccatttgg ctcacgattc ctaattttgc tgccatatac tgtaaaagca
     7081 tagcaatcag actagacatc aaaataactg acatcaataa atattggaaa ttttgtcctc
     7141 cggtgatcga agtagaccag ttacccgggt ccatataccc aactgctact aacgcaccag
     7201 gtccagagta agcaagaagt gttttaaaaa aacttttccc ctttggtaca tcaactgttc
     7261 catttacttc agctaaagat aaaccatttg tatgttcaat aagtcgatgc ttatgaaatt
     7321 gtttattgga ttgattattc aagaaaaaac cacctttcct atttatctac gattacattc
     7381 tacacctcaa aaagaaaaaa ttaaagaaaa agattcggta cgcctaattt tttttcatgg
     7441 cgcgtcttgc tagatgacga aagaaatttt gttctcctct aaaaaaacca ctattttttg
     7501 catgaaacta aagaatacgc ctttcttttt tcaataaact ttagtaaaat aagtcattga
     7561 gagcaattcg gaaatagaaa ggagagattt atatgttatc aacaggtatt gtcgtactta
     7621 ttgctattat tgctttgcta atcggcgcag taggtggctt tttcctagcg cgtaaatata
     7681 tgaaagacta tttagaaaaa aatcctcccg ttaatgagga catgttgcga tcaatgatga
     7741 tgtcaatggg gcaaaaacca tctgagaaga aaattcgcca gatgatgcag caaatgaaga
     7801 accaagggaa aaaataggtt ctgacccaac gactttttac gggctgattt tttcagtccg
     7861 ttttttattt cgtaatttgt tagactattc agattattca gcaaaaaaga caagcacaga
     7921 caatcctagg ctcttgataa agacaaagga ggagtaaaat gtcgattttt agaaaattag
     7981 gctggttttt tcgtcaggaa aagaaacatt atttaattgg ggttttctcg ctattcgcag
     8041 tggccttggt acagttagtt cctccaaaag tgattgggat catcattgat gagatcgccg
     8101 ataaaaattt atcaataaag atcattttcg ggtggatctt cctattgatt atcgctgctt
     8161 ttttacaata tctttttcgt tatatctggc ggacgaatat ttggggaagt gcagctcgat
     8221 tggagaaaga acttcggcag cagttattca aacattttac agaaatggac aatctctttt
     8281 accaaaaata tcggactggg gatttgatgg ctcacgcaac gaatgatttg aatgcgatcc
     8341 agaatgtagc aggagctggt atcttgacct ttgcagattc agtgattacg ggcggaacaa
     8401 cgatcattgc gatgattttg tttattgatt ggcgcttaac actgattgcg ctgattcctc
     8461 tgcctttgct ggccgtcact tcacgagtgc tggggtcaaa actccatgat gcttttcgtg
     8521 attcgcaaga agctttttct agtatcaatg ataagacaca agaaagtatc actgggatca
     8581 aagtcattaa aacatttgga caagaaaaag aagatctagc ggattttgaa gaaaagattg
     8641 aagatgcaat tattaaaaac aaacgagtca attttttaga ctccttattt gatccattca
     8701 ttaccttgat tatcggtctg tcttatgttg caacaattat tttaggtgga aagtatgtct
     8761 tggaaggcac gatctcgatc gggcagatgg tctcatttat tagttatatc ggtatgcttg
     8821 tttggccgat gtttgcgatt ggtcgattgt tcaacgtatt ggaaagagga aatgcttctt
     8881 atgatcgcgt caatgaactt ttgcatgaaa agacgcatat cgtggaacaa ctgggtgcag
     8941 tgcagacccc ggcaaaaggc gagctgaatg tagctatttc ctcttttaat tatccgaaag
     9001 aagaatcacc agcattagaa caaatcaagt ttgcgatcca agaaggagaa acattaggta
     9061 ttgttgggaa aactggtgcg ggaaaaacga cgatcatcaa attactgatg cgtgaatacg
     9121 atgattatca agggaaaatc caatttggcg gacgttcgat caaagaatat tcattgaatg
     9181 ctttattagg atcaatcggc tatgtgcctc aggatcactt cttgttttcg atgaccattc
     9241 gagataatat ccgtttttct aatccaaaac tttcagaaga agaagtagag catgcggctt
     9301 ctttagcatt tatcaacaat gaaatcaagg aattaccaaa aggttatgat accttagttg
     9361 gagaacgtgg agtctcttta tctggcggac agaaacaaag gatttcgatt gctcgagcat
     9421 tgatcaaaaa tcctgaatta ttaatattag atgatgcact ctctgcagta gatgccaaaa
     9481 cagaagaagc tatcttgcat aatttaaaag aaataagaaa agagaaaaca actatcattg
     9541 cagctcaccg tttaagtagc gtgatgcatg caaaagaaat cattgttgtc gatcaaggga
     9601 aaatcattga acgaggaacc catcatgagc tgcttgcact aggcggctgg tatgaacaaa
     9661 tgtgggagaa acagcaatta gaagcgaaga tagaaggagg cgaggcctga tggatgaaaa
     9721 ttatcaatcg gaatggacaa aatcgattcc acttaaagaa cagaaaaaga tcatcaaacg
     9781 actgatgaag ttcgccaaac cttttagaag aacgttcatc gttgctatct tatttgcgtt
     9841 tgctttgtct gtgatcaatg tcctattgcc aaggatcatc caaactttta tggatgatca
     9901 tctggctaaa cagtcggcta cgacacaagt tattttgttt tttgctggta tgtacttatt
     9961 tggagtcata gtaaaaagta tcatttggtt tttccaatgg tatttgtatt ccatggcctc
    10021 attgaagacc tatcagtata ttagggtaaa attatttgaa aagctgcata cactaggtat
    10081 gcgttatttt gatcagacac cggcaggatc aaccgtttct cgagtgacga atgacacaga
    10141 aacgctattt gaattttggt acgtcttttt gatggtattg actgggatct ttgcagtcgt
    10201 ttcttcattc cttgccatgt ttcaaatcaa tggagaaatt gccttgtgga ctttggtgtt
    10261 tttgccgatt ttaggaatcg tcatctggta ttaccagaaa ttcagttctc gtatttaccg
    10321 aaatatgcgg gaaaaattaa gtcagctgaa cacgaaattg aatgaatcga tttccggcat
    10381 gcgtattatc cagcagtttc gccaagaagc tcgtttagct aaagaatttg aacaagtgaa
    10441 caatgagtat ttagacacgc gttatgcgat gatcaaaacg aattcgattc tgttaagtcc
    10501 gatcatcaat tttctatatg cattggcgat tgcgttggtg ttgactttgt ttgggatcga
    10561 tgcgttgcat tctcccgtag aagttggact gatctatgca tttacgacct atgtccaggg
    10621 attttttaat ccaatgtctc aaatgatgga ctttttgagc atcttcacag atgggatggt
    10681 tgctggtagt cgtatcttga agattatgga tacggaagaa tatgcaccac aacaaaaccc
    10741 tggagcagat caaatgatta cttcaggtaa gattgagttt cgcaatgtaa gcttttctta
    10801 tgatggagag cacaatgtat taaacaatat ctcttttgtt gctaatccgg gagagaccgt
    10861 tgcattagtg ggacacaccg gaagcggaaa aagctcgatc atcaacgtat tgatgcggtt
    10921 ttacgaattt tatgaaggtc agatattgat tgatgatcat gacattcgag aatatccaat
    10981 ggaagagtta cgtaagaaaa tggggctggt cttgcaagat gctttcttgt tttatgggga
    11041 tatcgcagga aatattcgga tgatgaatcc agctatcaca gacgaacaga tacgccatgc
    11101 ggctgagttt gttcaagcaa acaaattcat cgatgaatta ccaaaaggat atcactcaaa
    11161 agtgatcgaa catggtgcca gctattctag tggacaacga cagctgatca cttttgcgcg
    11221 aacaatcgtg actgatccta aaattctagt attggatgaa gcaacagcaa atatcgatac
    11281 tgaaacagaa ggattgatcc aagaaggatt ggcaaaaatg cgtcaaggac ggacaacgat
    11341 tgcgattgcc catcggctgt caacgattcg agatgcagaa ttgattttag ttttagacaa
    11401 aggtcatata gtagaacgcg ggaatcatga aacacttcta gcgcaaaatg gtttgtatgc
    11461 agatatgtac aaactgcaaa gcagtggtat ataaaagatg ttgtgacagt agaattttga
    11521 cctaagcact agagtgaaaa taaaacatag cttctcatat ttttcatctg tgagagttag
    11581 tgtcgtagtc accagattta aaaaaagatt cgacagctag tcagaagtgc ctaatagcca
    11641 ctaagaaagc gttgacaaaa gcatttgctt tcgataatat aaagaaaacg atacaacttg
    11701 gatagtgatt gcctgatgca agctaaacct gatgagtctc ccatggtttc atggacggct
    11761 ttttgggttt ttctttttgt tttggataca gccctataca tcatggaaag gagcaaaaca
    11821 tgctgattga gaaagtactg aataacaacg tcgtaataac cagggatgaa agaaatcagg
    11881 aaatgatcgt gatgggaaaa ggtttggcat tcaaaaagaa agtgggtgac ccgatttttg
    11941 actcatgcat cgacaaaaaa tttcagttga caaatgacgg gctttcacaa cagttccaag
    12001 aattggcaca agcgattcct cttgagtact tagaaatttc ttgtgaaatc attgaatata
    12061 caaaagaggc tctacaagtc cagttgaatg attctgtgta tattgcattg acggatcatt
    12121 tgtatacggc tatcgaacgt cacaaaatgg gaatatttgt accaaatgta ttgctgtatg
    12181 atatcaaaca actatttcct aaagagtatg ccatcgggaa aaaaacagtg aagaaaatct
    12241 cggataaata ccacatcaag ttatcagaag atgaagccgg atttatcgca ttgcatttag
    12301 tcaatgcaca aggcattcaa agccagcaag acaataccta cctgtttact ataacaatac
    12361 aggatattct aaatatcgtc cgctattact ataagattac atttgatgaa gattcagtcc
    12421 atttttttcg attcgtcaca cacttacgtt tttttctgac acgcgtccta gatggagaaa
    12481 aatcagaagt gggagaagtt tctgaggaat tgtttcaggt tattcaaaaa aaatataagg
    12541 atacgtttgg ttgcgttgag aaaatagcag actttttagc cgagacgttc aactaccaga
    12601 tgtccacgga cgaaaaaatg tatttaacga tccatactgc gcgtttagtg catgagaatc
    12661 gttgtaaata aaaaaaggag gataaattaa caagaacgta tgaaataagg gtagtgacat
    12721 tcagttggct aaaccttcca ctaataaatt ggagggaata gaaatgggaa aataccaaga
    12781 attagcgaga aaaatcgtag aaaatgtcgg cgggaaggaa aatatcaatg gtttgacaca
    12841 ttgtatcaca aggcttcgtt tcaaattaaa aaacgaagaa aaagcaaatg atgagatctt
    12901 gaaaaatatg gacggtattg taacggtaat gcgagcggga gggcagtatc aagttgtcat
    12961 tgggaatcat gtgccagtcg tgtttgaaga ggttataaaa gcaggaaacc ttacttttga
    13021 tgaggctgtt tcaacagaaa aaatgagacc gtttgatatg cttattgatg gaatatcggg
    13081 ctgtttccag ccattccttg ctatcctagc tgctggcggg atgattcgtg gactgacagc
    13141 attccttgtc ttcttaggcg cgtttgaccg cggttctggc acatttgtta tgtttgataa
    13201 tatcggtgat tctgtattcc aatttatgcc tgttattatc ggtttaacag ctgcccgtaa
    13261 atttaaagta aatgaattcg tagggatgtt gcttggcgca gcattaatga atcctagctt
    13321 gtcgctggaa gctttgagtg gtgcagcaga agcaccacta acgaccatat tttctggcac
    13381 tatttttgaa gcacctattt atcaaacagt attcggcatt ccctggattg cgcgtaacta
    13441 cgcttcttct gtcattccca ttatctttat tgtgttgtta gctagccaaa ttcagaaacc
    13501 aattaaaaag ttagtacctg agatgatcgc gaatttcttc gttccattct tcaccgttct
    13561 tattacaatg cctttaggtt ttttactcgt tggaccagta ttcacattcg caactgatat
    13621 tttaatggct ggatttgaaa cacttttagc actttcgcca gttatttatg gagcaatcgt
    13681 aggcttcttc tggcaaattc tggtgatgtt tggtctccat tgggcaatcg taccaatggg
    13741 attgatgcaa ttttctgtga atggttggca aaatattatg acacctgttg cagtagtgtc
    13801 ttttggacaa actgcagcac ttaccgcact ctactttaag cttagaaatc caaaagacaa
    13861 agcaattgca ataccagcca ttgtttctgg tattgtgggg attacagagc cagctattta
    13921 tgggtttaca ttaccacgta aaaaaatatt tatttttacc tgtgtcggtg gtgcgttagg
    13981 gggcgcgtat agtgggttaa tgaatttaac ttcttggaat caaggtggac taggcatctt
    14041 tacaattccc aactatatta gaccagatgg tgatttaaca gatgtgatta atgtattgat
    14101 tgggatcgcc attgcgatgg tggtatcctt tacgctgact tttttctttt ggaaggatga
    14161 agcaggtgaa acagatgata tacagaaaaa atcaggtaaa gaaattgtaa aaacaccgat
    14221 acaaggacaa atcgctccac taaatgcagc aaaggatgca gcttttgcac aaggaacact
    14281 aggtcgagga atcttgatct accctgaaaa aggagaagta cgtgcccctt ttgacggaac
    14341 agtcatgacc ctgttcccga cgaaacaggc aattggaatg gtttctgaaa cagggctgga
    14401 attgctgatt catgtcggat tggatactgt gcagttggaa gggaaatatt ttgaaagctt
    14461 agtgcagcaa ggataaagag tgaaacaagg cgatctgttg ctacgcttcg acaaaaagaa
    14521 aatcgaaaag gaaggataca gtttagaaac acctgtgatc gtcaccaatt acacggatta
    14581 tttggatatt ttggaaaacc aaggggagac ggtcgggcca gacgatgcac ttatcaccgc
    14641 actgacctga acggttaggt gaagaatgga ctaggcttaa ctattctttt tattccactt
    14701 ctttattttc aaaattgaaa ggctctttca tgatggctga gctgataggg cgtttatcga
    14761 atttttgacc gacagaaatt agatggacta aattctgttg ataattttct gcaaaaacac
    14821 aactatataa cacgtctaga agataacaaa ttgatgtatt gatcgtaaaa ttaccaattt
    14881 ttgaatacag tcgctcgcgt gtcgtaagtc gcaaagcggc tgtactcatt gcagatagcg
    14941 ttgaatcacc gatactggtc aaagctaaaa ttggaacatg attttgtttc aatatttctg
    15001 cggtacgaag gatcacattg ttttcacccg tataagaaat cagaatcccg cacatatcac
    15061 tcgtgcaatt gtacgcttca tagaatgatt caccaaaagt ggtgacaatc gtgacatcca
    15121 ttttgatgcg cttcattttc agcatgaaat cttgagaaat caaagtattc gagttactgg
    15181 cgaaaatcac gatttttttt gcttgaagca ttagttgttt ggctttttgc agttggtcat
    15241 gatgaatgag agaaagtgta tcatgaatcg tcatctgttc taaagaagcc attttatttg
    15301 cgatcgtcat aataccgtct gtggtggaaa aaggaagatt cgcatctacg tcttgaaaat
    15361 tcccttcaag ataggctgtt tcagctaaaa aagcattacg aaaactgacc cagccatcaa
    15421 aacccatttt ttttgctaca cggatgagtg tagaggggtg gacataacat cgctcagcaa
    15481 tcgcttggat ggtcatttcg ccaagtgctg gagaatcgct taagagaaac tggatcactt
    15541 cattttctga aggagaaaag gagatttctt tgattttttc acgtaataac atgtagatac
    15601 cttcttctta tttttttacg taaattataa cttttttgaa aatgatttgt aaacggattt
    15661 atactatata acttaaaata gacatttttt gacaagatat ttacaaaaat ccactagaaa
    15721 acagtaaatt ttgtaaatgt tactggaaag aaacagattt ctattgtttg ttttgtttac
    15781 aaaaaagata atgaaagtgt aaaaaagtaa aggagtgaga acatggcaaa aggaatcaaa
    15841 atcgctacga ttggtggagg cagcagttac acccccgagt tgatggaagg atttatcaaa
    15901 agatatgagg aattaccgat acgagaaatc tggctcgtag atgtagaaga aggaaaagaa
    15961 aagcttgaga tcgttagtca gatgtctcag cgtatgtggg atgcttctcc gtatgaagtc
    16021 aagatctatg caactctgga tcgggaagaa gcattgaaag atgcggattt tgttacgact
    16081 cagtttcgtg tcggtcaatt agatgcacga atcaaagacg agcggatccc tagttattat
    16141 gggatgattg ggcaagaaac aaatggtgct ggcgggatat tcaaagcttt tcgtaccgtt
    16201 ccagtcgttt tggcaatcgt tgaggacatg aagcgtttat gtccaaatgc ttggctgatc
    16261 aatttcgcga acccaagtgg gatggtgaca gaagcagtta tcagatatgg caaatgggat
    16321 aaagtgattg gattatgcaa tgtgccagtg atggcgatga tgacagaacc agcaatgctt
    16381 ggcaagaaaa aagaagagct gatctacaag tttgccggtc taaatcattt ccattggcac
    16441 aaagtagcag ataataaagg aaatgaccgc acggcagaat tgattgatcg tttatacgat
    16501 gaagagaatg gattaccgaa aaatatcttt gatatcccgt ttttcaaaga acaattaaaa
    16561 caaatgaaca tgatcccttg tggctaccac cgctattact atcgtgaaga agaaatgctg
    16621 gaacatgctt tagaagaata tcaaacgatt ggtacaagag cgcagcaagt gaaagaaaca
    16681 gaagcagagc tatttgaatt atacaaagat ccagaattag accataaacc tgaacaattg
    16741 caaaaacgag gtggcgctta ttattcagac gctgcctgtg aaacgattgc ttcaatctat
    16801 ggaaataaag aaacacagat cgtggtatct acaaagaatg atggagctgt tcctgacctg
    16861 ccggcagatt gtgtagtaga agtcactgcc tatgttggcg cacaaggagc gagaaatgta
    16921 gcgtttggtt ctcttcctgc tgctgaaaga ggctggttgc aagtaatgaa gaatatggaa
    16981 ttgctgacga tcgaagcagc tgtgactggt gattatggga cagctttaca ggcgtttacg
    17041 atcaatccac ttatacgatc aggaaagaca gcaaaacgga tcatggatga gttgtttatt
    17101 gcacaccagc agtatttacc gcaattcaag gagacaatca gccgattaaa agaagaagga
    17161 atcgaagttc aagatgaact ggcaagagaa ttatcaggta atacacatat aatggaggaa
    17221 gtataatggg attcagaaaa gattttttat ggggcggtgc tacagcagcc aatcaatgtg
    17281 agggcggtta tgatgaaggt ggacgcggac tagcaaacgt agatgttgtg ccaatcggaa
    17341 aagaccgttt tcctgttatc accggaaaga aaaaaatgtt tgaatttgat gaggatcatt
    17401 tttatccagc aaaaattgca atcgatatgt atcatcgtta taaagaagat attgctttgt
    17461 ttgcagaaat ggggttcaag acttatcgaa tgtccatcgc ctggagtcgt atttttccaa
    17521 tgggggatga gaaagagccg aatgaagctg gcttgaaatt ttatgaggat gcctttaaag
    17581 aatgccaaaa gcacggaatt gagccacttg tgacgattac gcattttgat tgtccaatgc
    17641 atttgatcaa agaatatggt gcgtggcgtt ccagagaact cgttggtttt tatgaaaatc
    17701 tgtgtcgagt catcttcaat cgctataaag gacttgtcaa atattggctg acattcaatg
    17761 agatcaatat gatccttcat gcaccattca tgggcgcagg gctgtatttt gaaaaaggag
    17821 aaaatgaaga acaaatcaaa tatcaagcgg cacatcatga gttggttgca agtgccatcg
    17881 caacgaagat tgctcatgaa gtagatccgg agaacaaagt aggctgtatg ctggcagcag
    17941 gagcatatta tccatacact tgcaatcctg aagatgtttg ggcttctaga aaatcagatc
    18001 gagaaaatta tttcttcatt gatgtccagt cacgaggaga atatcccgct tatgcattga
    18061 aagaaatgga gcgaaatggc attcagatcg aaatggaacc aggggatgcc gcattgctga
    18121 aagaacatac tgttgatttt atctcttttt cttattatgc ttcccgagtt tcgactaccg
    18181 acaacgaact cttggaacag actgcaggaa atatctttgc ttctgtcaaa aatccatatc
    18241 tgcaagcaag tgaatggggg tggcaaattg atccattagg cttgagaata acgatgaatg
    18301 atatttatga tcgttatcaa aaaccattgt ttattgtaga aaacggctta ggtgcagtcg
    18361 atacgccaga tgaaaatggt tatgtggaag atgattaccg aatcgattac ctggcagcgc
    18421 atatccaggc gatgaaggat gcagtagaac tagacggagt cgatctatta ggctatacga
    18481 cttggggatg tattgattta gtatcagctg gtaccggtga gatgaagaaa aggtacggat
    18541 tcatttatgt cgatcgtgat aaccaaggaa atggcacatt agcccgttca aagaaaaaat
    18601 cattcaactg gtataaaaaa gtaattgatt caaatggaga aaatctttcg aacgattgat
    18661 caatgattta taaaagtcta ttgtttttca gcggattttt cgagtataat aaagctattc
    18721 gagaaacgca agcagaacaa tagacttttt ttcgcattgg ctgtaaagta tgcttacttg
    18781 attaagtaaa ggagaatcta catatgtatg atgtaatcgt tattggcgca ggtcctgctg
    18841 ggatgactgc tgctttatat gcttcacgtt caaacttatc cgtattgatg atcgaacaag
    18901 gtgcccctgg cggccaaatg aacaatacgg cagaaatcga aaattatcct ggattcgatt
    18961 cgatcatggg ccccgatcta gcttataaga tgtatgaaag tgtctcaaga tttgggacag
    19021 aaaatgctta tggaatcgtt caagatatca aggatcacgg cagccataaa gaagtcatct
    19081 gtgaagataa aagttacgaa ggaaaaacag tcattatcgc aacaggatgt atccaccgta
    19141 aattaggcgt acctggcgag gaagaatttg ctggacgagg cgtttcttac tgtgcagtct
    19201 gtgatggtgc gtttttccgt aacaaacgtc tagtagtcat cgggggtggg gactcagcag
    19261 tagaagaagc catctatttg acacaatttg cttctgaagt agtgatcgtc caccgtagag
    19321 atgaattacg tgcacaaaag atcatccaag accgtgcatt tgcaaatgaa aaaatttcct
    19381 ttatgtggga tacagtcgtt gacgaaatcg ttggaaacga aatggtagtg acaggtgtga
    19441 aagcacgtaa tgtcaaaacc ggcaatgaat ttgatttagc agcagacgga gtattcatct
    19501 atgttggact ggatccatta actgcacctt ttagagaagc aggtttgacg aacgaagctg
    19561 gctggattcc aacagacaac gaaatgaaaa cgaatatgcc aggcgttttt gccatcggag
    19621 atgttcgtga aaaagatttg cgacaaatca ctacagctgt tggtgacggc ggtattgctg
    19681 gacaacaagt gtataaatac ttggagtctt tagaagaaac aacagcataa gtttaaaaag
    19741 agtagaagaa cgggttcaat tgttctttta ctcttttttt tcgcgtgaaa tttactgaat
    19801 ctgatttatg cgaacacgtc caacaccttt tgataaaaga atcctttctt gatttaagcg
    19861 ttttttggtc ctcatacttt catatctaat aagtgaatgc tatacttatt aagaaccata
    19921 tttcagaaag aggtgttatt ttcatgtctt gggaacaagt gtatgaacaa tgggtacatg
    19981 aagaaaattt agaagaaaac ttaaaaaaac aattaaatga tctgggtgaa gatcctgaaa
    20041 aacttgaaga tgctttttat gcaccgttgg aattcggtac tgcaggtatg cggggaatct
    20101 taggacctgg gatcaatcga atgaacatct atacggtaag acaagcaaca gaaggactag
    20161 cacgtttcat ggatacacaa ggtgaagaaa caaaacgccg aggtgtcgct atcgcttatg
    20221 actcacgcca tcagtcacca gaatttgcaa tggaagcagc caaaacacta gcacatcaca
    20281 atatcccgtc ttatgtattc gaaagtctgc gtccaacacc agagctttct tttgcagttc
    20341 gtcatttgaa gacgtttgcg ggaatcatgg taaccgcatc tcacaaccca gcggcttaca
    20401 atggatataa agtctacgga gaagatggcg gacaaatgcc tcctgcagac gcagatgcat
    20461 tgacgaaata tgtccgtgaa gtcagcaatc cgctaaaagt cgaagtatta tctgacgaag
    20521 aagctaaaca tagcgggttg atcacgatca tcggagaaga agtggacgct gcttatctag
    20581 aagaaatcaa agcagtgacg attgatcgtg aattggttga aacaatgggg aaagatttga
    20641 aacttgtgta cacgccactt catggaacag ggaaaatgtt gggcgaacgt gcgttgaaac
    20701 aagctggatt cgaacaattc gtactagttc ccgaacaagc tgtggcagat ccagatttca
    20761 ctacggtcaa atcgccaaat ccagaagaac attcagcttt tgaatatgct atacgacttg
    20821 gagaaaaaga aggagctgac ttgctgattg caacagatcc tgatgcagac cgtttaggcg
    20881 cagcggttcg tttgccagat ggttcttatg aagtattgac tgggaaccaa atcggagcat
    20941 tgtttgctca atatattttg gaagcacatc aatcaagagg cactttacca gaaaacgcag
    21001 cggtattgaa atcaatcgtt tccagcgaat tgcctacagc aattgcggaa cactacggtg
    21061 ctaccatgtt caatgtgttg acagggttta aattcattgc tgaaaaaatc caacaatttg
    21121 aagaagacca ttcacatact tttatgtttg gattcgaaga aagttatggt tatttagtta
    21181 aaccttttgt tcgcgataaa gacgcgatcc aagcattgct tttattagca gaagtagcag
    21241 cttactataa aaaacaaggc aaaacgttgt atgacggctt gcaaacgatt tttgaaacct
    21301 atggctattt cgcagaaaaa acgatttctg tgacaatgag cggacaagaa ggttctgcga
    21361 aaatctctgc tttgatggaa aaattccgta aagaagcacc gacagaattt gccggagcaa
    21421 aagttattca aacagaagac tttaaagaat tgacgagaac agaccgtgaa ggaaacgtag
    21481 aaaaactaac aacaccgcca tccgacgtat tgaagtatac attggaagaa aatggctgga
    21541 tcgctatccg cccttcagga actgagccga aaatcaagtt ttatattggt gtaaaaggaa
    21601 gctctacaga agacgcagcc gaaaaagtag cctcttatga agcgattatc aaaaagatga
    21661 cagaagaata aaaaacttga gactgatgga aaaagtggta aactttagaa gtcagtcttt
    21721 tttcaagttg gaggtgaatg gattgccaga cacgcaaaaa tcaaaagtag agaaagtcag
    21781 tcaattattc aaaatgttga gtgatcccac tcgtcttaaa attctgctat atctgaaaga
    21841 cggggaacaa aatgtgacag caatcaccca agcagtggaa atggagcagt ctgctgtatc
    21901 gcatcaacta cgtttattaa gagaaaatca tgtcgtcaag tctcatcgcg agggaaaagc
    21961 gattttgtat agtcttgatg attctcatgt actggatatt ttgaaccaga cactcaaaca
    22021 tgtggaacat caataacgat aggtgaaaat agataaacac tggagtgtga caacgaagaa
    22081 atttcgttgt cacactccag tgttttttaa gaaaaagaca aaaatgatta atcgcttttt
    22141 tctgttgttt cagttccata ataactatga tcttctaggt ctaaagcatt caaaataagc
    22201 gaagccgttt cttcaataga taaagatgcc acattgatca ctacacatcc tagtttttca
    22261 taaagatcat tcgcaaaatc gagttcagct ctaatttttt cgatatcgga ataagcagta
    22321 tcagggttta atccgtaaga gcgcatccgt tctttacgga tattatttaa aatatctggg
    22381 ttattcgtta gtcccacgat ttttttagga tcgatttccc acagctgttt gggaatatgt
    22441 gcttggggaa tcaaaggtaa attagcaact tttaaatttt tatttgctaa aaacaagctc
    22501 aaaggcgttt tggaagttcg ggatacgcct agcaaaacga catctgcttc taaaaaacca
    22561 cgaggatctt tcccatcatc gtattttaca gcaaattcca ttgctttgat ccgtttgaaa
    22621 tagttctcat tcaataaatg gagggcacca ggttgccgaa ttggcttgat accagttaaa
    22681 cgttcaattt ctgatatcgg tggcgtcatc acatcgaaac taaataattg atgttcttca
    22741 caaaaagaac gtgttatctc gatcagttct tgattgatta atgtatgcag gacaagtgct
    22801 ttttcttcta atgcatcttg caatgcctgc cttaatgttt tttcatctgt cacgaacgtt
    22861 ctgcggaata ggttgaactc aacggtagga tactgagcca tagtagcttg cgctagtttt
    22921 gtcgcagttt ctccagcaga gtcggagatg acaaaaaaag taacgatttc tgtagaatcg
    22981 gaagacatgg ataaaacctc acttgcattt tttttatgtt tattatacca tactctagaa
    23041 tgcgtacttc gtaatcattt tgatttggag gaataagtca tgaaaaaaca gacattgatc
    23101 gaacaatcat tagaaacatt gaaaaataac ggttttaaat atactaaaaa aagagaagca
    23161 ttgttaactt atttagtgaa gcgtaaccgc tacgtatctg ctaaagaagt ttttgactat
    23221 atgaatcagg aattcaaagg ggtaagttat gatacgattt accggaatct ccatgatttt
    23281 gcagagatcg atttactgga agaaaccgat ttaaatggtg aaatgaagtt tcgttttcat
    23341 tgctgtcagg gggaaatcgg ccatcaccat cattttatct gtacagtgtg cggaacaacg
    23401 aaagaaatcc atatgtgtcc aatgaattat ttcgaagaac agctaggtgg atgcacgatc
    23461 gaagggcata gattcgagat tttcggaaga tgtgaaaatt gtcaataagg atttaaataa
    23521 aataacattt gataatttaa aaaaaaagca agatttcctt gacggtcaaa acagcattag
    23581 atataatatt tacaggacag tcttgttttt tgagatatat ttcaataaca attacggtta
    23641 ctttttaagc tcggagggag ggaattaaca tgtcaaaaac agtcgttcgc aaaaacgaat
    23701 cacttgacga tgctcttcgt cgcttcaaac gttccgtgtc aaaagcgggt actttacaag
    23761 aatctcgtaa acgtgaattc tacgaaaaac caagtgtaag acgcaagaaa aaatctgaag
    23821 cagctagaaa acgcaaaaaa ttctagttct gtgatgaatt ggagttggta gtgtgtcact
    23881 actaagtaga ttaaacgatg acatgaaagc agcgatgaaa gcgaaagata aggaatcttt
    23941 acaagtcatc cgaatgatca agtcatctat acaaaacgag cagattaaag aaggtcacga
    24001 cttgacagaa gaagaagaac taacggtgtt gtctcgcgaa atgaaacagc gacgtgattc
    24061 tttgcatgag tttgaagaag caggacgtga cgatttagct gaaaaagtaa aaagtgaaat
    24121 tgtaatagtc gaaaaatata tgccggaaca actttccgac gaagaaattc gacaacttgt
    24181 tcaagaagca atcactcaaa ctggtgcttc atcaatgaaa gaatttggca aagttatggg
    24241 tgcaattatg cctaaagtca aaggaaaagc agatggaaac caagtgaatg cgattgtgaa
    24301 agaactatta caatcttaat tttatcattt aaactgcgga cagtgattgt ccgcagtttt
    24361 ttgtgttcgt aaaaaaagaa ctaaaaaaca gctagaagaa agaataagat tctttcttct
    24421 agctgttttt ttcctgttta atatctctta tgttaaattg tgtttgattt ggttattctt
    24481 ctggtagttc atttgcttct tgtttatacc ctgataggcg ataggcgtaa taaaagagta
    24541 agctgccagc gtaaccaagt aacgttggga ccatccagcc aagtcctaat gcagcaaaag
    24601 gaacgtgatg atgataaaat cctagtaagc ttgctacgac agtttgctga ctcactaatg
    24661 caggcgaatt tgccaacata tccaatacgg ctggaacggc agaaaagaac atcgttactt
    24721 gatagacaaa tggactcttt tggaagaatt ttgctgttaa ggaaagcaag attaacgcaa
    24781 gtgacaatgg atacaaaagc atcaatactg gtaaagacca ttggatgata ttatccaatc
    24841 cggcattcgc aacgacaaaa gagacaaaag tcgttaaccg cagccatgtc atataactga
    24901 ctttagggaa cagtttatgg aaatcttgtg caaaagaaac aacgagtccc atagcggtag
    24961 tgaagacacc taatgtgacc attaccccta agaagctagc accgaagtta cctaaataaa
    25021 aattagtgat ctgagaaagt gcagttccgc cattttctga taaaggcatc ttattcaaac
    25081 tcatcgcacc gagaaggacc agaccaaaat aaatcatgac ttctaacgca acgctcaggc
    25141 taccagcttt tgctgttaat tctgtgactt ttttatcttt aaaaccaaga tcacgaactg
    25201 catggacaaa agtgacactt aaagcaagta aagctactgc atcaaccgtg ttgtatcctt
    25261 ctaaaacgcc atttgtcaaa gcgttcgtct gataagaatc tgcagcagat tggtttagat
    25321 tccccatggg ttggaataaa gaaacgacaa aaatgatagc taataatgca agaaacaagg
    25381 agtttaaata ctttccgaca tatttgacca aacgtgttgg atgtacagta aggaaataag
    25441 caagtaaaaa gaaaagagca gagaataaaa acatacctaa tgaatcatat ttttcaggca
    25501 aaaagggttt agcagccatt tcaaaagcag ttgctgctgt ccgcggtgta ccaaagaatg
    25561 gtccgatcgt taaatgaaca aggatcaaga acaaggcagc ataccatttt ccgacaggtt
    25621 tagctaagtc atacagacca tcactttttg tcactactac agctaagata gctaataatg
    25681 ggaaaagagc gcctgagatg gcaaatccta caccagcgct gatccagttt gatccagcca
    25741 tttgacctaa gtggacagga aagatcagat tacctgaccc gaagaacatc ccgaagataa
    25801 gtgaactgac catcaataat tgcttccatg aataagtttt tgtttcttcc ataaattttt
    25861 cctccaataa ttatcatttt gagtaacaaa ataaacgttg cagaaactag aaatgccact
    25921 aatatcagat tccatgaaaa ccaccacctt aaaatataaa aaaaagccct tttccacttg
    25981 ggaaaagggc tctagattct agaacggtac cacctttttt cgcttaaaaa agcttcttca
    26041 ttcacggacg cttttcgata aagttatccg ctagcctgat aatggaggca gccacaatga
    26101 tctacgtttt cgacattgac tcggaaatga tgttcgtcta ttcgatttat tgtcatttct
    26161 cacttacatg actcactggg aataaatcgc tgaatagcct actgttttcc tcgacgcttt
    26221 gctgtatatt tatttgttga cgttataata cacctgtttt ttttgaaatg caagaatatt
    26281 tcaaatattt tgttttcttt ttttgaaaca agaaaaatac ccagagaata cttgctaagt
    26341 tagacggaat acttgtcctc tatcttatgc atagcttaca tgatcagctt tttttattca
    26401 aacgagacct ttagagaatg gaagcagcta ttttttaacg tttcttggta cttatggtta
    26461 agcaactctt ttagaacctt ttcttatttg ctatatagtt tcttctgagt atggtaaaat
    26521 aggaaagaat gaatcgaaaa aaaggggatg tctatttttg acggaagagt ctagttcttt
    26581 agagattaga ttgacggctg ctgatgatgt cagcatgctt ttaggtgcac acgacaagca
    26641 tataaaactg attgaagaaa caacagaaac aaccatccat acacgtggag aaatcattca
    26701 gatcattggc gaacaatccc agatttcctt ggctgcctca gtcattcgga cattgcagga
    26761 attgatcaaa cgaggaatcc atgtaagtac accagatgtc gtgacggcac tgaaaatggg
    26821 gcaaaaaggc acattagatt attttgttga aatgtatgag gaagagatcg tcaaagaccg
    26881 taatggaaaa ccaatacggg taaaaaacag cggccaaaag aaatatgtcg aatcggtagc
    26941 caaacatgat atcgtatttg gcgtaggacc agccgggaca ggaaaaactt ttcttgcagt
    27001 agtattagca atcgctgcct tgaaaaaagg tgaagtgcaa aaaatcattt tgacgcggcc
    27061 tgctgtggaa gcaggggaaa atctaggctt tcttcccggg gatctaaaag aaaaagtaga
    27121 tccttatctg cgtcctgttt atgatgcttt ataccaaatt tttggattag atcatacgaa
    27181 ccgattgatg gaacgaggcg tgatcgaaat tgctccttta gcttacatgc gggggcggac
    27241 attggatgat gcattcgtta ttttggatga agcacagaac acgacagttg ctcagatgaa
    27301 aatgttcctg acacgtcttg gttatcaatc aaaaatgatt gtcaatggag atacgagtca
    27361 gattgatctg cctcgaggga caacaagtgg tcttgttcat gcagaacgaa cattaaagca
    27421 aatcaagaaa atcgattttg ttaattttga agcaagtgac gttgttcggc accctgtagt
    27481 tgccgaaatc atcaaagctt atgaaaaggc agatctccat caagaataag ctttgcttag
    27541 gaggataagc acatgttgaa taaaacactg cagaaactac aagaaaaact tggaaaggca
    27601 gcattgcctt tcctgctgtt cttgtttttt atcattatct gcggcgttac atttagtagt
    27661 gtcaaacaaa aggccaatga tttcagagaa ggccaagtag cagaagaaag tatccgtgcg
    27721 aacaaaacga tcgagaacac ggaagaaacc gaacaaaaaa gaaaactggc agcagaagca
    27781 gtcactccag aatacactta ccaaaaagat cttgcagatg accagaacaa tcggatcaag
    27841 caactatttg aattgatcga taaaacgaat gatgccatca acaaatctta tgacgaaaag
    27901 attgataagg caaaggaaga tgaaaatatc ccaaagccta ctacagaaga gcggatcgcc
    27961 gcgttgaaga aaaattttga aaatgtgaat gcagaaaatg ttgcgtttta tcaaaaacta
    28021 ccgaacaatt tttattcgac gatatttgaa atgactgaaa ctgagatcca tactgtgagg
    28081 gatgagagtc tgaagctcat tgacgaacag atgagcaaac aagttcgtga aagtgaattg
    28141 gaagctttta aacaagaagc agaagagcaa atccaatatt tgaatgtcac accagcacaa
    28201 caacaaatga ttcgctactt agtggatcaa ggtatcgtag tgaatgacgt attgaatgag
    28261 aaaaagacag aagaattgaa acaatccgca cgagaagctg tacaaccagt gatgatcttc
    28321 caaggagaaa tcattgttcg tgaaggtaat caaatcgatg cttcagctat gaaaaaattg
    28381 gaattactag gactgactaa tcaaacaact tcgatttttc cgctaattgc catggtttta
    28441 gctgttttgc ttcaaatcgg cgtactgatt tataattcac ttcaattcca aaatgcagga
    28501 gaaagaacaa aatacgtgct tttctatgtg acagctatgt cagtcagcat agtgctgatg
    28561 aaatttttcc aattattcca aacggaacaa gctacttata tcccgctgtt ttatcccgct
    28621 gcttttgcac cacttgtttt aagctttttc ttgaaccgaa gagcagggat catggctggt
    28681 attttccaag cagtatctgc acttttcatt ttttatgatt cgattggcac gaactttcta
    28741 acgattatct tgatgtctta tgttttctct gggctcatgg caacgattgt cagacgcaaa
    28801 cggatctcag aacaaggatt atctgctgcc atgtgggtca ttatttttcc tgtgatgatg
    28861 gatgtcattt tgatcgtcta tcaaggaatg agctttggtg atgcaacgac ttggttaatg
    28921 cttgtctgcg ggttcactgg tgcactcttc tcattcttgc tgacgatcgg tctacaccca
    28981 tatattgaat tgatggtcaa tgatgacagt gtaattgtat taaatgaact cagcaatcca
    29041 aatcatcctt tattgaaaca gttgctggaa gaagcgccag gtacttatca tcatagtatg
    29101 atggtagcta atctaagtgc taacgctgta gcagaaatcg gcggacgttc tttgctaact
    29161 cgagtagctt gctattatca tgatatcggc aagatcaaac atgctagctt tttcgttgag
    29221 aacttgcctt caggagcgga aaacccgcac aatttcttat tgccagaaga cagtaaacat
    29281 attatctttg gacatgtgac agatggagcc aaaatattgg aagattataa tatgccgcaa
    29341 atggtgatcg atatttgcaa acagcatcat ggaaccacgc taatgcgtta tttctacatc
    29401 aaagcgaaag aacgcaatcc ggaagtaaca gaagagcaat tccgttatcc tggaccaaaa
    29461 ccgcaaagtc gtgaagcagg gatcgtcaat attgctgaca gttgtgaggc tgcagtacgg
    29521 gcaatggatc atccgacgag cgaaaaaatc gaaagttttg tccataatct tatcgaagag
    29581 cgtatttccg atggtcaatt agacgatagt ggactgacct tgaaagaaat ccgaaaagtg
    29641 gaaaaatcat tgatcagcgg tttaagtagc accttccatt ctcggatcaa atatcctaaa
    29701 atgaaaacag aagttgagaa gataaaggaa gaacaagaag aatagaaaga agcgatgtat
    29761 aatggatatt acctttatgg atgaaacaaa ccaagtctca gaagagaaaa tgaaagaaat
    29821 cgatgacttg ctgcaatttg cagcatctta tttaaagctg ccagaggaaa cagaaatgtc
    29881 tgttactttc atggacaatg cggcaatcca agtaatcaat cgtgattacc gtggaaaaga
    29941 tgcaccgaca gacgtcatca gttttgctct ggaagaagaa ggcgaagacg aaatcccagt
    30001 gatttttgaa gatggagaga atccattgcc tcgtgtacta ggagacatca tgatttctat
    30061 tgaacgtgct gctgaacaag cagaagctta cggccattca tatgatcggg aacttggctt
    30121 tttagcggta catggattct tgcatatcaa cgggtatgac catatgacac cagaagatga
    30181 gaaagaaatg tttggtttac agaaagagat cttggatgcc tatggactca aaagataaaa
    30241 acgtagagaa aaacaaacat ttcctgatgt ctgtggaatt tgctgtccaa ggagtcaaaa
    30301 ccgtttttga ggaagaacgc aatatgagga aacatgttgc gttcggtatc cttgctttat
    30361 ttatgggcgt gattttccag cttgatcgga tagagtggtt atggctgctt ttagctgttt
    30421 ttctcgtttg gatagtagag atcatcaata ctgtttttga aaatgtagtg gacatgttta
    30481 cggattttca ttttcatccg attgggaaaa aaatcaaaga tatggcagct ggagcagttt
    30541 tagtgaccgc ttgttttgct gtcatcattg ggttgatttt atttgtccct aaaatatatc
    30601 atttgttgtt taattaaaaa ggagaacgta tatgccagaa cataaatcag ggtttgtcgc
    30661 aatcgtcggc cgcccgaatg taggaaaatc cacattattg aaccgaatcg tgggtcaaaa
    30721 aattgccatt atgagtgaca aggcacagac aactagaaat aaaatccaag gagtatatac
    30781 tacaccagaa gctcagctga tttttatcga tacaccagga atccataaac caaagcatcg
    30841 tctaggagat tttatggtag aagcagctta cagtgcgttg aaagaagtgg atgcagtctt
    30901 atttatgatc agtgctgatc aaaaacgcgg ccggggagac gattttatta ttgaacgtct
    30961 aaaaaacgtg cattctccag tttatctgat tatcaataaa atcgataaga tccatccaga
    31021 cgagttgctg ggaatcatcg aagattattc gacgcagatg ccttttgcac aagtgattcc
    31081 gatctctgct acagaaggaa acaatgtcga acgcttaatg gatgtattag tcagtgaaat
    31141 gccagagggc ccacaatatt ttccagatga tcaagtgact gatcatcctg aatactttat
    31201 cgtttctgaa ttgatccgtg agaaagtctt gtttttaaca cgagacgaag tgcctcattc
    31261 tgtcgcagtc gttgttgatt cgatgaagcg aaacgagaat gataaaatcc atatccaggc
    31321 cacgatcatc gtcgaaagag atagccaaaa aggaatcatt atcggcaaag gcggaaaaat
    31381 gctgaagcaa gtaggaacga aagcaagaaa ggatatcgaa aatcttttag gagacaaagt
    31441 atttctagaa ctttgggtca aagtccaaaa agactggcga gacaaaaaag tctatctgca
    31501 agactttggc taccgaaaag acgaatactg atattctgaa cttcttatag gttaagtata
    31561 gaaataaact ggataaaaat ccgcacgttg agaaaaacgg cggaagatcg aaacataaat
    31621 aatctggatc ggcagaatct gcttttccag atttattttt taggagggag aatatgagcc
    31681 agcttgcaga atcgaaaggc ttgattttat ctagccgtaa ttacaaagaa aaagataaat
    31741 tagtcaaaat ctttacggaa tcttctggca aaatgatgtt ttatatcaaa ggaatccatc
    31801 ggcaaaatca gccattagct ccggctttgc tcccctttac cgaagcagtc tatatcggta
    31861 attttcgaag tgaaggtttg tcgtttttaa gcagtgtcaa agacgtccat gcgtttcgga
    31921 acatccaaga agatatcttt ttgagcggtt atggtaccta tttgctaaat ctagtagatg
    31981 cagcaatcga agatcatcaa tatgacccgc atttattcca atttacgcaa caagctctgc
    32041 agcggatgga tcaaggagat gatgcagaaa tcatcacgaa tatctttgaa gttcaaatac
    32101 tgcaacgctt cggaatcgcg ccaatttgga cgcactgtgt cgtatgcggg gaaacaaaag
    32161 gaaaatttga ctattcttcc aaatctggcg gtgtgatatg cgagaaacac tggccaatgg
    32221 acgaacaccg ttatcatgca gatcctcgcg ctgtttattt cgttcgaatg ttttcggcta
    32281 tttcttatga taagatcagt ggaatccact tgaaagaaga aacgaagcag gcaatccgtg
    32341 acctcattga ccagttgtat gatgaatatg tcggtattca tctaaaaagc aaaaagttca
    32401 ttgatcaaat gaagtcttgg gcacacgtat taaagacgcc agagaaaaat atgcctgaaa
    32461 aagagatacc tgaaaaagaa gaataaatga aagatacaac aaaaaaatca aaactatttg
    32521 acaaatacag ggacttccgt ctattattaa ccgtagatta aatagatcgt tcactgagag
    32581 gatctattat ataaacaaga aaactgcaaa atgaatacgt acattgacca agatcagtaa
    32641 aaaacacgtg gtttagcgag ttcgggagag tggaagccgg acccaaacga tttttgaaga
    32701 ggcacttgcg agtacgataa atgaagctgc cgaatcttta ttcggaagta gggtggaacc
    32761 gcgaaattat tcgtccctat gtctgtaatc agacataggg actttttgtc gttttctttc
    32821 ctgtattctt attgacagca acgcttaaaa attgaaaggg gcaaaacaga tgagtaaaaa
    32881 attgaccgtc caagatatga ttttaacatt acaaaaattt tggtcatcaa acggctgcat
    32941 gctgatgcaa gcttatgaca cagaaaaagg agcaggtacg atgagcccat atacattctt
    33001 acgggcaatc ggaccggaac catggaatgc tgcttatgta gagccatccc gccgaccagc
    33061 tgacgggcgt tatggagaaa acccaaaccg tttgtatcag catcatcaat ttcaagtcgt
    33121 aatgaaacct tcacctgaaa atatccaaga gctttattta gaaagcttgc gtcttttagg
    33181 aattgatcca ttagaacacg atattcgttt tgttgaagac aactgggaga acccatcgat
    33241 ggggtgtgcc ggtgtgggtt gggaagtatg gcttgatggg atggagatca cacaattcac
    33301 gtacttccag caagtcggag gactaccttg taaaccagtt acttctgaga tcacttatgg
    33361 actagaacgt cttgcttcat atattcaaga agttgaaagt gtctatgatc tggaatgggc
    33421 tgatggtgtg aaatatggcg agatcttcaa acaaccagaa tatgaacatt ccaaatattc
    33481 ttttgaagta agtgaccaag acttgctact aagcaacttt gaccgttttg aaaaagaagc
    33541 taaacgctgt atcgatgaaa atcttgttca tccagcgtat gattatattt tgaaatgcag
    33601 ccatacattc aatttattag atgctcgcgg tgcagtatcc gtaacagaac gagcaggtta
    33661 tctttcccgt atccgcaaca tggctcgtgc tgtagccaaa atctttgtgg cagaacgcga
    33721 aaaattagga ttccctttat tgaataaaaa cacagcaaat ccagaaaaca tgaaggaggc
    33781 tgaataagat ggcaaaagac ttattacttg aaatcgggct ggaagaaatg cctgctcatg
    33841 ttgtcacacc aagcatgaaa caattagaag aaaaaacagc aaaattttta gatgaaaacc
    33901 aattgactta tgatacgatc gaaacgtttt ctacaccgcg ccgcttagca atcaaagtaa
    33961 caaacattcc agaacgtcaa gcagacagtg aagaagaagt caaaggacca gccaaaaaaa
    34021 ttgctttgga cgcagatgga aactggacga aagcagctga aggatttgtc cgcggacaag
    34081 gtctgacgac agatgctatc acttttagag aattaaaagg cgtggaatat gtctatgtaa
    34141 cgaaatacac tcacggcaaa gaagcgaaag aagtattgac aggtttagca gatgtcatta
    34201 aaagcctcac tttcccagta acgatgcatt gggcagaata cgattttgaa tatatccgcc
    34261 ctattcactg gatcgtggca ttgttagatg acgaagtaat cccgtttgag atcttagatg
    34321 tcaaaacagg cagagcctct cgaggacatc gtttcttagg cgaagatgtg acatttgcgc
    34381 atgcaaaaga atatgaagaa aaattgaatg aacaattcgt tattgcttca ccgcaaaaaa
    34441 gacaagaaat gatcgttaca caagcaaatg aattagccga aaaaaatcac tgggttattg
    34501 atctagacga agatttacta gaagaagtca acaatttagt cgaatatcca actgtctttt
    34561 ctggcgactt cgaagaaaaa tatctttctg ttcctgaaga agtactagtc acttctatga
    34621 aagaacatca acgttatttc gaggtacgga acgatcaagg tatgctgctt cctcatttta
    34681 tttctgctcg taatggtgat cgggtaaaat tggaaaatgt ggttcgaggc aatcaaaaag
    34741 tattgacggc tcgtttagaa gatgcggaat tcttttataa tgaagacaag aaagcttcaa
    34801 tcgaccaata cgttgaaaaa ctaaaaactg ttactttcca tgaaaaaatc ggtagtatct
    34861 atgagaaaat gcagcgggta caagtcattg cccgtatcat tggcgaacaa gtaggactat
    34921 ctgaggaaga attgatggat ctgcaacgag ctgctgaaat ctataaattt gatctagtaa
    34981 ccaacatggt aggggaattt cctgaactac aagggatcat gggcgaaaaa tatgctctgc
    35041 tgaaaggcga gaaacctgct gtagcaacag cgattcgtga gcattacatg cctatatcca
    35101 gtgaaggaga gctacctcag tctacagtag gttctgtttt agcgattgca gataaattag
    35161 atagcatttt ttctttcttt gcagttggga tgattccaag cgggtcaaac gatccttatg
    35221 ccttacgtcg ccaagcatac ggtgtcgttc ggatcatcga atcgaaaaaa tgggcctttc
    35281 ctttgtctgt tctgcaagaa acgattagtg atgttatcag taaagatacg gatcgtttcg
    35341 gcattggttt atcagaaggg caacaacagg tgatcgactt tatcaaaggc cgtctgcgcc
    35401 aattattgac aacgaagaat attcgtcatg atgtcatcga agctgttttg aatgcagaac
    35461 aaaaagatct gacaaaagta tttgcagcag cacaattgtt caagcagcat cttgcagatg
    35521 aagacttcaa accatcgatg gaagctttga cacgtgtcgt gaacttagcg aaaaaagcgg
    35581 agcttgaaaa acaatcagaa gtagaccctg aactgttcga aaatgaagcg gagaaagaac
    35641 tgcacaaagc agtagaggca atgaaaaaaa gctttgctga ccaaacgatc aacgaaaatt
    35701 atgatcagct tgtaagcctt cgcccactga tcgaaaatta ttttgatcag acgatggtca
    35761 tggcagaaga accagcagta cgtaagaacc gattaacgca attaagtcaa attgctgcca
    35821 tggctttgtc gctagcaagt ttagatcaaa tcatcacaaa ataatttcta atatgtaaat
    35881 aaaccagaga ataggcacaa aa
//
LOCUS       DAZULO010000016        35002 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000016 DAZULO010000000
VERSION     DAZULO010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.016"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            164..1309
                     /locus_tag="ACG8OA_000837"
     CDS             164..1309
                     /locus_tag="ACG8OA_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS4975671.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1446..1790
                     /locus_tag="ACG8OA_000838"
     CDS             1446..1790
                     /locus_tag="ACG8OA_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFS4975672.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            2061..3347
                     /gene="efmA"
                     /locus_tag="ACG8OA_000839"
     CDS             2061..3347
                     /gene="efmA"
                     /locus_tag="ACG8OA_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFS4975673.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3485..4084)
                     /locus_tag="ACG8OA_000840"
     CDS             complement(3485..4084)
                     /locus_tag="ACG8OA_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4975674.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4488..5609
                     /gene="mnmA"
                     /locus_tag="ACG8OA_000841"
     CDS             4488..5609
                     /gene="mnmA"
                     /locus_tag="ACG8OA_000841"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFS4975675.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5855..7276
                     /gene="arcD"
                     /locus_tag="ACG8OA_000842"
     CDS             5855..7276
                     /gene="arcD"
                     /locus_tag="ACG8OA_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFS4975676.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7290..8408
                     /locus_tag="ACG8OA_000843"
     CDS             7290..8408
                     /locus_tag="ACG8OA_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFS4975677.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8468..9760)
                     /locus_tag="ACG8OA_000844"
     CDS             complement(8468..9760)
                     /locus_tag="ACG8OA_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS4975678.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            9910..10470
                     /locus_tag="ACG8OA_000845"
     CDS             9910..10470
                     /locus_tag="ACG8OA_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4975679.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            10599..10976
                     /locus_tag="ACG8OA_000846"
     CDS             10599..10976
                     /locus_tag="ACG8OA_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFS4975680.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            10909..12981
                     /locus_tag="ACG8OA_000847"
     CDS             10909..12981
                     /locus_tag="ACG8OA_000847"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS4975681.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(13192..14139)
                     /locus_tag="ACG8OA_000848"
     CDS             complement(13192..14139)
                     /locus_tag="ACG8OA_000848"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4975682.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            14385..14849
                     /locus_tag="ACG8OA_000849"
     CDS             14385..14849
                     /locus_tag="ACG8OA_000849"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFS4975683.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(14911..15954)
                     /locus_tag="ACG8OA_000850"
     CDS             complement(14911..15954)
                     /locus_tag="ACG8OA_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFS4975684.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(16331..17296)
                     /locus_tag="ACG8OA_000851"
     CDS             complement(16331..17296)
                     /locus_tag="ACG8OA_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFS4975685.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            17530..18375
                     /locus_tag="ACG8OA_000852"
     CDS             17530..18375
                     /locus_tag="ACG8OA_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4975686.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            18566..19753
                     /locus_tag="ACG8OA_000853"
     CDS             18566..19753
                     /locus_tag="ACG8OA_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS4975687.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            19759..20115
                     /locus_tag="ACG8OA_000854"
     CDS             19759..20115
                     /locus_tag="ACG8OA_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFS4975688.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            20117..20455
                     /locus_tag="ACG8OA_000855"
     CDS             20117..20455
                     /locus_tag="ACG8OA_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFS4975689.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    20531..20787
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            20850..21884
                     /locus_tag="ACG8OA_000856"
     CDS             20850..21884
                     /locus_tag="ACG8OA_000856"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS4975690.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            21877..22563
                     /locus_tag="ACG8OA_000857"
     CDS             21877..22563
                     /locus_tag="ACG8OA_000857"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS4975691.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            22587..23435
                     /locus_tag="ACG8OA_000858"
     CDS             22587..23435
                     /locus_tag="ACG8OA_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4975692.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            23632..24405
                     /gene="sufC"
                     /locus_tag="ACG8OA_000859"
     CDS             23632..24405
                     /gene="sufC"
                     /locus_tag="ACG8OA_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFS4975693.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            24418..25704
                     /gene="sufD"
                     /locus_tag="ACG8OA_000860"
     CDS             24418..25704
                     /gene="sufD"
                     /locus_tag="ACG8OA_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFS4975694.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            25701..26936
                     /locus_tag="ACG8OA_000861"
     CDS             25701..26936
                     /locus_tag="ACG8OA_000861"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFS4975695.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            26923..27393
                     /gene="sufU"
                     /locus_tag="ACG8OA_000862"
     CDS             26923..27393
                     /gene="sufU"
                     /locus_tag="ACG8OA_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFS4975696.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            27398..28789
                     /gene="sufB"
                     /locus_tag="ACG8OA_000863"
     CDS             27398..28789
                     /gene="sufB"
                     /locus_tag="ACG8OA_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFS4975697.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(28884..30026)
                     /locus_tag="ACG8OA_000864"
     CDS             complement(28884..30026)
                     /locus_tag="ACG8OA_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4975698.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(30065..30799)
                     /locus_tag="ACG8OA_000865"
     CDS             complement(30065..30799)
                     /locus_tag="ACG8OA_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4975699.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            31031..31306
                     /locus_tag="ACG8OA_000866"
     CDS             31031..31306
                     /locus_tag="ACG8OA_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4975700.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            31404..33401
                     /locus_tag="ACG8OA_000867"
     CDS             31404..33401
                     /locus_tag="ACG8OA_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975701.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(33477..33812)
                     /locus_tag="ACG8OA_000868"
     CDS             complement(33477..33812)
                     /locus_tag="ACG8OA_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4975702.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            33939..34154
                     /locus_tag="ACG8OA_000869"
     CDS             33939..34154
                     /locus_tag="ACG8OA_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4975703.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            34191..34553
                     /locus_tag="ACG8OA_000870"
     CDS             34191..34553
                     /locus_tag="ACG8OA_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFS4975704.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            34599..34925
                     /locus_tag="ACG8OA_000871"
     CDS             34599..34925
                     /locus_tag="ACG8OA_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975705.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT        11518 a         6213 c         7340 g         9931 t
ORIGIN      
        1 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
       61 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
      121 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
      181 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
      241 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
      301 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
      361 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
      421 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
      481 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
      541 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
      601 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
      661 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
      721 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
      781 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
      841 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
      901 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
      961 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     1021 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     1081 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     1141 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttggaagcaa tgtatggaga
     1201 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     1261 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     1321 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     1381 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     1441 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     1501 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     1561 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     1621 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     1681 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     1741 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     1801 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     1861 gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg
     1921 ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa
     1981 ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga
     2041 aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga
     2101 gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc
     2161 aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg
     2221 ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg
     2281 atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc
     2341 ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt
     2401 ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc
     2461 cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat
     2521 cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca
     2581 attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg
     2641 tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg
     2701 caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg
     2761 gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag
     2821 attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa
     2881 tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta
     2941 ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg
     3001 atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat
     3061 atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc
     3121 gtgtcttagg gaacttcaac tctttgatga acctgacagg tccaattgga ttattatttg
     3181 caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga
     3241 ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat
     3301 tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt
     3361 tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc
     3421 tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt
     3481 tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag
     3541 ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc
     3601 ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc
     3661 ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt
     3721 cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc
     3781 taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca
     3841 agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat
     3901 attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg
     3961 cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag
     4021 caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat
     4081 tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact
     4141 cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca
     4201 attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc
     4261 ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt
     4321 tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa
     4381 ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc
     4441 ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca
     4501 gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt
     4561 tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg
     4621 acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc
     4681 aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg
     4741 aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca
     4801 aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag
     4861 ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg
     4921 gagtagacaa taataaagat caaacttact tccttagcca attatcacaa gaacagcttt
     4981 caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac
     5041 gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa
     5101 agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc
     5161 ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc
     5221 aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag
     5281 atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca
     5341 caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca
     5401 agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg
     5461 atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag
     5521 cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc
     5581 aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc
     5641 taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat
     5701 ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga
     5761 ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt
     5821 gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag
     5881 caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat
     5941 cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt
     6001 tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga
     6061 tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat
     6121 cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct
     6181 gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat
     6241 tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg
     6301 agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt
     6361 aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt
     6421 atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg
     6481 ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa
     6541 ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt
     6601 gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc
     6661 aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc
     6721 agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat
     6781 gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg
     6841 aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa
     6901 tctattctta ttgactttct tagttactga ttatgcctat caatttgcct attctttatg
     6961 tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt
     7021 aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat
     7081 gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg
     7141 tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa
     7201 agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg
     7261 ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat
     7321 ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat
     7381 tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac
     7441 gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag
     7501 tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg
     7561 gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc
     7621 ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg
     7681 ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca
     7741 aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag
     7801 atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga
     7861 tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct
     7921 atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta
     7981 ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca
     8041 cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag
     8101 aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat
     8161 gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca
     8221 ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag
     8281 gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact
     8341 gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac
     8401 aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca
     8461 tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt
     8521 tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt
     8581 tgaccaaaac aagaagagaa gtcgaaagaa agacgccacg ataaccaaat gcactggaaa
     8641 cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac
     8701 tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag
     8761 ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat
     8821 tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta
     8881 ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg
     8941 caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca
     9001 gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga
     9061 tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct
     9121 gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca
     9181 gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc
     9241 caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac
     9301 cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa
     9361 gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta
     9421 attgatacac actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag
     9481 cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga
     9541 taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg
     9601 acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa
     9661 atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt
     9721 ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa
     9781 aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc
     9841 ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga
     9901 aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa
     9961 ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat
    10021 atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta
    10081 acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt
    10141 ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa
    10201 gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga
    10261 atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa
    10321 gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt
    10381 tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg
    10441 atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag
    10501 atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact
    10561 aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt
    10621 tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag
    10681 aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc
    10741 atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg
    10801 gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt
    10861 tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta
    10921 attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa
    10981 aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta
    11041 ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg
    11101 ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta
    11161 aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat
    11221 gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa
    11281 ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag
    11341 caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa
    11401 gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat
    11461 ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa
    11521 atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa
    11581 gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt
    11641 gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg
    11701 gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat
    11761 ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc
    11821 gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa
    11881 gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga
    11941 gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt
    12001 ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat
    12061 caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca
    12121 aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa
    12181 gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg
    12241 agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca
    12301 gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca
    12361 gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca aggactgcaa
    12421 gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat
    12481 ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga
    12541 accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc
    12601 aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat
    12661 ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac
    12721 taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa
    12781 atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca
    12841 gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc
    12901 ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta
    12961 aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt ggtcagcttc
    13021 aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat
    13081 ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt gacaagactt
    13141 ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat attattggtg
    13201 aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa caggacgctg
    13261 tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac ataacaattg
    13321 gattccacca atgaacagga agatcgagac catggaaggc catcctgatg taggatcacc
    13381 aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga cacaagacaa
    13441 agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga taccatcaat
    13501 cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac gttcgcggtt
    13561 ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt ttgaaaatcg
    13621 gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca agcggaaatc
    13681 acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt agaatttttt
    13741 cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg tgccgacaca
    13801 atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg gcggatcttg
    13861 cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag
    13921 agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag gataagctga
    13981 ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata
    14041 ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag gaacagtttt
    14101 ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc tttcaatcgt
    14161 atgtttatat atggtttaca ttcaaaattt aataaacctt aaataacacc tttaaaattt
    14221 gattttacgt aaaagaacag gtattctttt tgtaaactaa cgtatttagt ttaccatgtt
    14281 tcctcggttt atcaaacttt taagaacatt ttttattagt tggcaagtag ttttggatag
    14341 agaaaaagga ggaagttcca gtctattgac taggagcaaa caaaatgaaa atacacacac
    14401 ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta tcttttcagc
    14461 caatttcaga aagcgaatca gcgaatcagc gaattttaac gcgcacacaa gaacagtttg
    14521 aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata aagagaggta
    14581 cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga gaaatccgaa
    14641 cgtataaaga agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg attgggcagg
    14701 caaatcgaac caatccattg ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg
    14761 gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa cgccaattgt
    14821 tgttgttaga aggatatctg caaaattaaa aacagttgtg attcggacat tgttccatgc
    14881 gagaatcaca actgtttttt catgttcatt ctaaactttg atcagccaaa taaatagtaa
    14941 agccatcact gtttggagaa gcccgacagc taatccataa gataagaagc gtgtaccttc
    15001 tttcatgaat tttgcaaagt ctaaacgcag accaattgcc gctaaagcag tgatctcaaa
    15061 ccagctgctg ataaaatgtg ccgattggtc aataactaca ggtaaagaaa acaagctgtt
    15121 gaccaaacaa aagatcacaa accctaatac ataccaagga agcggtaatt ttcgtttttt
    15181 ctgcgtatca gttggcgttt caaacagagc tggttgtttc ttgtgcatga atttttcaaa
    15241 cagatagaca acaacaacga gcatcataat acgtagaatc ttaaatagca tggcgaattc
    15301 gacaacagaa ttgtcaagta agcttgcccc agcgactact tgaccaacag attggagtgt
    15361 accacccaaa agtgcacttt tttcaatgag atttccacca aacaactgta atccaagaaa
    15421 agggagagtc aacatcatca cagtacccag tagattgact agtgtgatga tctgtccttt
    15481 ttcttcctct ttcgcatgga tggaaggggc gattgaagca atagcagaag aaccacaaac
    15541 agcgtttcct ccagccatca tcaaagaaga agtatcgctg aattgcagtt ttttaccaat
    15601 caagtaagca ccgatgatag tgactgacat ttgcagtaac acaaaaacaa ttccttttac
    15661 accaatttga gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa cagagaattc
    15721 taatagtttg ctttcagcaa attttgtgcc gattgctaga acaggctgtt taaaaaaagt
    15781 atttcctaat aaaataccca ataaaatagc aatagtcgct ccacctaact gtggcagata
    15841 aatagcaaga agcctgctga aggcagcgat ggcaaaagaa agcagtaaac cgggtaagat
    15901 ttcgcggacc gctgaatcct ttacagattt tattggatgt gacaatgatt tcatttgatc
    15961 ggttcctttc gtcaataaat actaaaagca tactcgaaca tagtagcact aaaccagtat
    16021 tttgacaaaa gaacaagagg tcgtgacaaa agtcttgtac gacctcttgt ttcgaataaa
    16081 cggtgttcca gaagtagctt ctacggaaat aagccgaaat ttcacaaaaa tttgagaagc
    16141 aattttcgta aattccttct tatttcttga agctgaccac ttcttttacg acctcttggt
    16201 aaacggtgtt caaaagcttg catctgcggt aataagccca accagtacaa aaacatgaac
    16261 agctgttttt gtttggccgt tcttattact tgatgcagaa cagctttttc acaacctatt
    16321 gaatcgatta ttaagcgatt tttttagaaa caaattttcc atgtttgcta tgttctttaa
    16381 ttaagaaacg tacgtaatga gcaagaacgt agaagacaac tacacttgca aacagacatt
    16441 ccgcaacttt taagaaatcc aatacgctac tatagatacc taaactgttg aagtaatgaa
    16501 cagtagtaaa tatagctgtt gcagagataa caagcgggaa tgtaaaggct gcatagcttg
    16561 gataaaaacg aagcttgaac aaacgtggaa gcatagacag cacaacgaaa tacaatactt
    16621 gagataaagc gaacaatgct acgacaagat aaaaattagg ttcagcaaag tttttaagat
    16681 aacctgttag gcaaagagaa ccaggtgctg caacgatcgt aatcagcgga cgtgtcggat
    16741 gagccatatt tcttacgaaa aagacacgtt tgatgatgat cggcaataag aacgcatagc
    16801 agatcaatga aatccaaaag aaaatttttc caataagtgg gaagaagttc ccggcagtta
    16861 cagtgatgac cccaaatcca acgtaaatga taaaccagct tggataaatt gcttcaattg
    16921 aaagatcagc tttgataaca tggtgataat tgaaataaaa gacaaggatc acttggaaga
    16981 taacagcgca cagccaaatg aatttgacaa ttggtgcaat actttcatat gaaacaaaat
    17041 aagtacaaat aaccatgagt gacatcgtaa aagttggtga aacggaggcg atgattggat
    17101 catgcaacgt ttgtttcgtg tgttcgaata agataaacaa tttaacaaaa atgaatatca
    17161 taagaatcat agagataccg ccagttagat tgcctaaagc aacatggtga tagtctttaa
    17221 acagatttcc aagggaagct aacccaagga tcaacccgca gatcgggatc ggaataattt
    17281 tcaaaaagtt cttcatatat aatattctcc tttttattta atagatttta gtaccaagat
    17341 gaacgaatac attgtaaaat gaataggaat gtatgattac gagcaagcag acagctgatt
    17401 gtcttcatct aagttactag ttggatcagc aactgttttg ttgtacattt ctattataga
    17461 tggttttttg tatatttaaa tcaaatagtt tttatagtaa ttatcataaa aagttatgga
    17521 agtggtgcca tgtttaagtt attaacgact tttcgagcag tatatgaaac aagaaatttt
    17581 tccagagcag cagaagtact gtttttatct caaccagcag tctcgaatca aatcaaacag
    17641 ttggagcggg aattgaatat cgatcttttt caacgtaacg gaagaaaaga gatgcttcca
    17701 actaagcaag ccgacttatt atatgaacgg acactggttt tgttagaaga atgggaagaa
    17761 actcagcaaa aattgctcaa tgaacaaaat gaagaagaag tatgtcggat cgttgcttcc
    17821 cacacctttg ctgtctatct tttgcctcag ttgatgaaac atctgatcaa aaggtttcct
    17881 aaagtgcatt ttgagatcga aatcacgaat tctcatgaat cattagaaca agtagcgaaa
    17941 catgaatccg attttggttt tatcgaaaag ccattagaga cttccggcgt acaaagatac
    18001 tctttgatgg aagaccagct agtcatcgct ggggacccaa aaagcaaatt gtggcttgta
    18061 agagaagcaa actctggtgt ttttcattac acgcagcgtt attttgaaga gatgaatatc
    18121 caagataaga agatgatcgt gaaaagtaac gatgtaattg ttgcgttgct gaaggaagga
    18181 atcggtcgct cgattttgtc taagcgctct gttccagaaa atgtgccaat gactccgttg
    18241 aatttccatc gatattttta tttcatccaa cgaaccaatc tggtttctga tcagctgcta
    18301 gagattgccg aagagatcct tcgatacaat gaaagcacag aaacttctca cagcgaggag
    18361 aatctcagct ggtagataat tgcatgtatc aaaaaaagtt ctatgataca ctaaagataa
    18421 gcagtaggct tttgtactgc atttgctcaa gcaactgcac aaacggaaag ttaatttttc
    18481 ccaaatgtct atggaaagac tttccattat ctaactggac cgctataata atcagcagta
    18541 tggtttttaa gaaaagggat gaaacatgga aaaaaaatta aaagcttcga acgataatcg
    18601 tattgattat ggcgtgattt taccagtctt tctgttatgt ttgattggtc tgctctcatt
    18661 atatgtagca cttagccatg atccgaacaa tccagcagtc ttgaaaggcg tagggatgca
    18721 agttgtctgg tatttagttg gagcggcagc aatcgttgtc atcatgcatt tgaattccaa
    18781 atggatctgg aaactgactc cttaccttta tggtttgggg ttagtggtaa tgggactctt
    18841 gctcaaattc tatgatcaga atttagctgc ttcaacaggg tctaaaaact ggtttcgatt
    18901 cggtgcgttt actttccagc cagcagagtt gatgaagatt gcgtacatct tgatgatggc
    18961 actgatcgta actaaacata atacgcaagt aaaagaacgg acgatgaaat cggacttttg
    19021 gctgattggg aagctcttga tcgtgacaat accggtttta gcattgatca tggcccaaga
    19081 tgactttgga acgatgcttg tctttcttgc gatctttggc gggattttcc tgatgtctgg
    19141 aatctcttgg cgcatcatcg cacctgtagt aatcttggca gtagtcgttg gagcaggaac
    19201 gatcttcttg gttacgactg aaggcggaag agacttgctt tataaagttg gattcaaatc
    19261 ttatcagttt gcacgtattg attcatggct ggaccctttc catgatacgt caggaatgag
    19321 ttatcagccg gcccaaggac ttttggcaat tggtaccggt ggcttgtttg gtaaaggatt
    19381 caacgtaagc aatatctatg tgcctgttcg tgagtccgat atgattttta ccgttatcgg
    19441 tgaaaacttc ggctttatcg gtggtgcatt cgtcattttc ttgtatttta ttttaatcta
    19501 tcgtatgatt cgggtgtgtt tcgatacgaa taatgaattt tacgcctata tcgcaagcgg
    19561 tttgatcatg atgctgctgt tccatgtctt tgagaatatt ggagcaaata tcggattact
    19621 tcctttaaca gggatcccat tgcctttcat tagtcaggga ggatcctcga ttttagggaa
    19681 catgatcggt atcgggttga tattatcgat gagataccaa aatgaagcac ctgcaagatc
    19741 ttcaaggagg taagaaaatt gtacaccttt tactggtacc cgaaatgttc aacatgcaaa
    19801 aaagccaaag cgtggttaga ccagcatggc gttagttatc aaaccgttga tatgatcaaa
    19861 gaagtaccga aggcctcaac gattgagact tggctagaaa cgagtcaagt accaatccgt
    19921 cgcctgttca acacaagcgg gatgaaatat cgggaacttg gactgaaaga tcaagtaggc
    19981 gagtttacgg cttcaaaagc gagccaagtc ttatcgacag atggtatgct gatcaagcga
    20041 ccactgatct tacgagagga ccagcttgtt gcaattggat tcaatgaaaa aatttatgaa
    20101 ggagaactga tataaaatgg caggcaagca tttgaagaaa aaggacaacc tttggatctt
    20161 atttaatgga aaagaatact gtgttggttt gacaaatgaa gcgcaagaag aacttggaga
    20221 catcacgttt gccaatctgc caaaagcagg acaatcgcta aaaaaagatg aacctctgat
    20281 cgaagttgaa gcggaaaaag cggtcaatga atttgccagc ccgctaacgg gtacagtttc
    20341 ttctgttaat gataaaatcg gtgaagatat caatatcttg aatgattcag atgaaatgaa
    20401 tgcgtggatc ttaagtttca aagatgttga tccaaaagaa tttgacgaat tataaaatca
    20461 gaaaatcact tgaaaaaagt tgacactgat tcttccagtt gctataatca taagcaatcc
    20521 tatatgaaac gagaaagctt tgaaaagaag agtacatgtc acggacgttt gaagagagcc
    20581 ctgtttgctg aaaatgggta acgaaaatga gatggaagat ggtcttagag cagagtaagc
    20641 gagcgtaagc aaacttacta cgggagtgcc cgttacagcg cgacagtata cggaaagaag
    20701 ctaagtccaa gtactggaag aggctgttat tgcgaagtaa cggtaaatca aggtggtaac
    20761 acggaagttc aagctttcgt ccttttgctt aaggcaagag ggcgaaggct tttttgtttg
    20821 aaaaatcata agagaaagat gtgaatggca tgtcattaat ccaactaacg aatgtcaaaa
    20881 agacattcaa tgggaaaaac ggaaacatcc aagccgtcaa tgacgtttca ctaaacgttg
    20941 aaaaagggga tatttatgga atcgtcggct attctggcgc aggaaaaagt acattagtcc
    21001 gtctcctgaa tggacttgag cttcccacga gcggcgaagt gatcgtaaac gatcaagata
    21061 ttacaaaatt aaaaaacaaa gaattacgta cattccgaaa gaaaatcggt atgatcttcc
    21121 agcatttcaa cctgttatgg tcaagaactg tacttgaaaa tatccagctg ccgctggaat
    21181 tagctggcgt accaaagagc aaacgtaaag aacgggcaga agaactgttg cgtttagtcg
    21241 ggttagaagg acgcggacaa gcatatcctt cacaactttc cggcggacag aaacaacgcg
    21301 tcggtatcgc acgagcattg gcaaatgatc ctgagatcct gctttgtgat gaggctacta
    21361 gcgcattgga cccgcaaaca acagaagaag tattagactt attgttagcg atcaataaga
    21421 aattgaattt gaccatcgtc ttgatcacac atgaaatgaa tgtgatccgc aaaatctgca
    21481 acaaagtcgc tgtgatggag ttggggaaag tcgtagaaga aggagacgta ttgactgtct
    21541 tcagacaacc gaaaaaagaa gtaaccaaac gctttgtaca acaagacatc gaaccagaag
    21601 ctgattcgac agaactatta gctgaactca tcaaagaaaa tccaagcggc cgtttggcaa
    21661 ctttgacctt tagcgaaaga aatgccaacg agccagtcat ttcccaagcg attcgacgct
    21721 ataacatcga tgtcaacgtt gtgtatggaa agatcaaaca agtgaaagaa ggttcctttg
    21781 gttcattgac tgtcttgatg actggtgaaa ccaaagaatt agatcaagca gaagtgttca
    21841 tcaaagaaca aggagtagga attgaggtga tccatcgtgg ataaaagctt ttcagaaaca
    21901 tatctagatt ttaaacaaat caacccggaa acgatgaatc aggctatcct tgatacacta
    21961 tatatgacat taatctcaat ggtcttagtg acgatcatcg gattagtttt gggattgatc
    22021 ctttactcta ttggacgcaa aaacaaaccg tatgctcgaa tcatttatgg aatcgtatcg
    22081 atcatcagta atattttccg gtcaacgcca tttatgatcc tgatggtttt gatcattcca
    22141 tttactaaag caatcgtagg cactatgcta ggtgcgcaag cagccattcc agctcttgtt
    22201 ttatccgcag caccatttta tgcccgtcta gtagagattg cttttcgtga agtcagccct
    22261 ggtgtactgg aagcagcaga tgcaatggga gcaagttact gggaaatcat ctggaaggtc
    22321 ttgatacctg aaagtattcc cgcattgatt tctggattaa cagttacaac gatctcgatg
    22381 atcggattta ctgcgatggc cggcgcgatc ggcgcaggtg gacttggagg tcttgcttgg
    22441 caggaaggct accagcgtgg taatctaaca gtgacattcg ttgcgacttt aattatttta
    22501 ctaatcgtat ttatcgttca aggaatcggt gatttcttaa ctaagaaaac agacaaaaga
    22561 taataggata tagggggatg taggatatga aaaagaaaat ttttggattt gcagcagcgc
    22621 ttttgctgac tgtaggatta gcagcatgcg gtaacaacag cgacagcaaa gattcagcaa
    22681 ataaagcaga cgacacgaca ttgaaagtcg gcgcttcacc gacaccgcac gcagagattt
    22741 tggaacatgt gaaaccatta ttgaaagatg aaggaatcga tcttgaaatc gtcaaattcg
    22801 atgactatgt attaccgaac caatctttgg aagaaggcga cattgatgcc aattacttcc
    22861 agcatattcc ttatttgaac aaagaaatca aagaaaaagg atatgacttt gtcaatgccg
    22921 gtgctgtcca catcgaacca atgggcttgt actctaaaaa agtcaaagat gtttccgaat
    22981 taaaagatgg tgcaacaatc attacttcaa attctgaatc agactgggga cgcatcatca
    23041 caattctaca agatgctggc ttagtcaaag tcaaagatgg agtagatctg gaaacagcaa
    23101 cattcgatga tattgaagaa aaccctaaga acttgaagtt caaccatacg atcgacccat
    23161 cacttttagc ttctacgtac caaaatgatg aaggtgacct agttgcaatc aacgcaaact
    23221 tcgcttacgg cgcagggttg aatccattag aagatgccgt attgcttgaa aaggacaact
    23281 ctccttacgt gaatattatt gcgactcgta aaggtgatga agacagtgac aaaatcaaaa
    23341 aactgattga agtgcttcac cgcaaagaag tccaagaatg gatcacagac aaatggggcg
    23401 gctctgttaa accagtaaag gctgatgcaa aataaggtat ggaacaaaaa agatgagaaa
    23461 taagtctgta atggcttatt tctcatcttt tttataccta ttctaattta gaaatcaaaa
    23521 ttgtttaata ataaataaaa gatatttgat tttagtaagg aaggtcattg cgcttcattc
    23581 tcaattagtc taaaatgagt gagaacgaaa gagaatttgg gggtataatg catgtctgtc
    23641 ttagaaataa aaaatctaca tgtgtcgatc gaggaaaaag aaatccttaa aggcgtaaac
    23701 ctaacaatga aaaccggaga aatccatgcc atcatgggac ctaacggcac aggaaaatct
    23761 accttatctg ccgctatcat gggcaatcca aactatgaag taacagaagg cgaaattttg
    23821 tttgacggga aaaatgtttt agacttggaa gtagatgaac gtgcgcgact tggcttattt
    23881 ttagctatgc aatatccaag tgagatacca ggtatcacga atgcggaatt catgcgtgca
    23941 gcgatcaatg cgaaacgtga tgaaaacgac aaaatttctg ttatggcatt tttgaaaaag
    24001 ttagatgaaa aaatggcttt actaaatatg ccagaagaaa tggctgaacg ttatctgaac
    24061 gaaggttttt ctggcggaga aaagaaacgg aatgagatcc tgcaattgtt gatgttagaa
    24121 cctacatttg caattttaga cgaaatcgat tcaggtctgg atatcgatgc tttgaaagtc
    24181 gttgctaaag gagtaaatga aatgcgcgga gacaatttcg gtgcattgat catcacccac
    24241 tatcaacgat tattgaacta tatcacacct gatgtcgttc acatcatgat ggaaggccga
    24301 gtagtcatga cagggaatgc tgacctagct aaacgtctgg aagcagaagg ctacgctggt
    24361 atcagtaaag aattaggaat cgactataaa gaagaagaag cgtaaggggg agcaagaatg
    24421 aaagaaaaaa actggctcga ttatcttgac gcggtaaatg atttttcttt aagtaaaggc
    24481 gaacctgatt ggatgagaac cttccgtcaa gacgcactag cgaaagcaga tgaactgcca
    24541 ttgccgcata tcgatcgggt gaaattccat cgctggtcgt tatttgatgt gaaagaaaca
    24601 caaaccatca gtgaaacagg cacgattccg gcatttgatg caatgaaaga caatcctgtg
    24661 cttgtccaac aaggttcatg gacgattttt gaacaactgc ctgtggaatt agcagaaaaa
    24721 ggagtcattt ttaccgactt gttcactgca atgatcgaat atccagaact agtccaagaa
    24781 tattatatga aaaaagcagt aaacatgaac gaagaccaat tgactgcact ccatgtagct
    24841 tttatgaaca gcggtatttt cctgtatgtg cctaaaaatg tcgtgatcga tgaaccactc
    24901 gagtccttat tcattcaaga tggcgcatcc gatgagcact tcttcaagca tgtgttgatc
    24961 gtggcagatg aacatagtga attttcttat ctggaacgtt tccagacaac caaagaacaa
    25021 gtagcaaaat catcaggtaa tatcatcgtg gaagtcatcg ctaaagctgg ttcaaaaatc
    25081 aaatattcag ccgtagatca attaggagaa aatattactt cttatatgaa tcgccgtggg
    25141 cacattttac gagatgcttc tgtcgactgg gcaattggtg tcatgaatga cggtcatgtg
    25201 atcgctgact ttgactcaga tttagcagga gaaggtgcac acgcagaagt caaaatcgtg
    25261 gcaatcagct cgggcagaca aatccaagga atcgatacac gagtaaccaa taaagcccct
    25321 catacgatcg ggcatatttt gcaacacgga gtgatccgag aaaaaggaac attgaccttt
    25381 aatggtatcg gccacatctt gaaaggggca aaaggtgcag atgcccaaca agaaagtcgt
    25441 gtcttgatgc tttctgataa agcacgtggt gatgcgaatc ctattctttt gatcgatgaa
    25501 aacgaagtaa ctgctggaca tgctgccagt gtaggacgag ttgatccaga agaaatgtac
    25561 tatctgatga gtagaggatt gcataaagaa gaagcagagc gcttagtgat tcgaggattc
    25621 ctcggttctg tcttaacagc aatccctgta gaacaagttc ggaaagaatt agtcgaagta
    25681 atcgaaggga agttgaatgg atgatcgatg ctaaaaagct acgcaatgat tttcctatct
    25741 tatttcaaga cgtcaatgat gaacctttag tttatctgga taatgcagcg acgacccaaa
    25801 agccaaaaca agtactccag gcattagcgt actattacga acacgataac gccaatgttc
    25861 atcgcggtgt ccatacgtta gcagaacgcg cgacccatga ttatgaacaa gcaagagaaa
    25921 aagtacgggc gttcatccat gcaaaagaaa cagcagaagt gctgtttact cgtggtacga
    25981 cgacaagctt gaattgggta gccagaacct tcggcgaccg ctatatcaaa gcaggagacg
    26041 agatagtgct ctcttacatg gaacatcatt ctaatgttat cccgtggcag cagttggcaa
    26101 aacgaacagg tgctgtcttg aaatatatcg aactaacaga agaaggcttc ctggatatgg
    26161 aggatgcagc gaaaaagatt acggacaaaa ccaaaatcgt ctccattgct catgtatcca
    26221 atgtcttagg tgtcttcaat cctgttcaag aattagctga acttgtccat gaaaacggcg
    26281 gggtcatcgt agtagatggt gctcaatctg ccccgcacat ggccattgat gtccaagcac
    26341 tagactgtga tttctttgct ttcagcggac acaaaatgtg cggaccaaca ggtattggtg
    26401 ttttatatgg taaacgaaaa tggttagaag aaatggaacc tgttgaattt ggtggcgaaa
    26461 tgatcgattt tgtcgaatta tacgacagta catggaaaga actaccttgg aaattcgaag
    26521 cagggacacc aaatatcgcc ggcgctatcg cattaggcca tgcaatcgat tatttgcaaa
    26581 aactcggtat ggacaatatc catcgatatg aagaagaact agccgcttat gtgttgccaa
    26641 aattacaagc aattgatgga ttgactgttt atggtccaca agacccgaat tttcggacag
    26701 gagtcgttgc attcaatttg gatgggttac atccccatga tgtcgcgaca gctttagaca
    26761 tggaaggggt agccgtcaga gcaggtcatc attgtgcgca accgctattg aagtaccttg
    26821 aagtatcagc gactgctcgc gcgagctttt atttctacaa cacaacgcaa gacgccgacc
    26881 gtttagttga agcaatcatg gcaacaaagg agtttttcca acatgggact atctaaatta
    26941 gacaatttat accgtcaagt gatcttggat cattcttccc atccgcatca tcgtggaagc
    27001 ttgactgact cgacaagcaa gatcgaattg aacaacccca catgtggaga tgtgatcgaa
    27061 ttgcaagtcg atgtaaaaga cggaatcatc aaaaatatcg cctttgacgg cagtggctgc
    27121 tccatcagta cggcaagtgc ctcgatgatg acagacgccg tcatcggaaa atcaatagaa
    27181 gaagcagaac atttaactgt agactttcta cagcttgtcc aaggaaaaga agtcaaagat
    27241 cccgaccaat taggcgatgc tgctatgctt ggtggtgtag ctaaatttcc cgctcggatc
    27301 aaatgtgcga cacttgcctg gaaagcacta ggaaaagcaa tggaaaacga tggtaccgca
    27361 gctttccatc attaccacaa ggaagaggag tgagaacatg ggcgtaccag aattagaaga
    27421 atataaattc gggtttcacg atgatgtcga accggttttc agtacaggag aaggcttgac
    27481 agaagaagtg atccgtgaga tgtcaagaat caaaggcgaa ccagaatgga tgcttgaatt
    27541 ccgtctgaaa tctctagaaa catttaacaa aatgccaatg caaacatggg gccccgacct
    27601 ttcagacatc gatttcgatg ccatcaaata ttaccaaaaa ccaagtgaca aacccgctcg
    27661 tgactgggaa gacgtaccag aaaaaatcaa agatacattt gaaaagattg gtataccaga
    27721 agctgaacga gcttatctag ccggtgcttc cgcacaatat gaatcagaag tcgtgtacca
    27781 taatatgaaa gatgaattcg aaaagttagg aatcgtcttt actgacactg actctgcttt
    27841 gaaagaatac cctgaattat tcaaagagta cttttcaaaa cttgtaccgc caaccgacaa
    27901 caaattagca gcgctgaatt cagccgtatg gtcaggtggg acctttattt acgtgccaaa
    27961 aggcgtcaaa gtcgatgttc cattacaaac gtacttccgg atcaatgcgg aaaacacggg
    28021 gcaattcgaa cgtacgctga tcattgttga cgaaggagca agcatccact atgtcgaagg
    28081 ttgtacggca cctacttatt caagtaacag cctgcatgcc gccatcgtag aaatctttac
    28141 aagaaaagat gcgtattgcc gttacacaac gatccaaaac tggtcagaca atgtgtacaa
    28201 cttagtaacc aaacgagcaa aagcatacga aggcgcaacc gttgaatgga tcgacggaaa
    28261 cctaggtgca aaaacaacga tgaaataccc aagtgtttac ttagatggaa aaggtgcacg
    28321 aggcaccatg ctctccatcg cctttgcagg agcaaaccaa atccaagata cgggtgcaaa
    28381 aatgatccac aatgcaccaa atacatctag ctctatcgtc tctaaatcaa tttcaaaaga
    28441 cggcggagaa gtaaactatc gaggacaagt aacctttgcc aaaaacagtg cgggttcgat
    28501 ctctcatatc gaatgtgaca cgatcatcat ggacgaatgg tcgaaatccg atacgatccc
    28561 attcaatgag atccacaaca gccaagtctc tttagaacac gaagccaaag tctcaaaaat
    28621 ctcagaagaa caactctact atctgatgag ccgaggcctg accgaatctg aagcaactga
    28681 aatgatcgtc atgggctttg ttgaaccatt caccaaagaa cttccaatgg aatatgcagt
    28741 cgaactcaat cgattgatca gttatgagat ggaaggcagt gttgggtaaa cctgttatag
    28801 gagctagaac ccttgatata taagggttct agcttttttg ttttgcctgt atactagaaa
    28861 tgaccagatt ttgaccagct cttttactga tgagccatca tatcagcaaa gtcttgacct
    28921 gttttcttag gtgcattatt tgtaatatgt gcatagatgt ctaatgtcac tttactattt
    28981 tcatgcccta atcgttgttg aactaccttg acagattctc ctgcttctaa taataaacta
    29041 gcatgtgtat gtctaaaccc atgaggtgta atacgaggca agttatattt tttcaaaata
    29101 tatcttaacc aatcattaac agtgtttgga atgtatagag tgtttttaac tgttgtaaaa
    29161 acatgttgct ctttagagct agtattgtat ccaagtttta aatactcttc tttttgctgt
    29221 aatttccagt tgttgaggat agaaagagtc tcagtatcta aagagataac tcgtctagaa
    29281 gctcttgttt tcggagattg aattataact ttaccaaatt catctacagc aagagtttta
    29341 ttgacatgta gttctttatt aaacgtgtct atgtctttcc actgtaaagc gagtacttca
    29401 ctctttctca tacctgtgta tgccaataat ctgaagaagg cgtattgttt catgttacca
    29461 aaatctttaa aagcatccag taaattatta agctcctctt ttgagtaaaa tttacgattt
    29521 ttctcatact ctttttttcg tggaagaagt gttttttcca taggattatt gtcaatatat
    29581 tgcattgcaa ccccgtagcg taaaatttgt gcagtctgtt ttctcatata atcgtaactt
    29641 tcatatttac tgtgccattc atctaccact ttttgacaat atgggacaga gatattcgat
    29701 agtttaagct taccaaatgc tggaagtatt tgattatttg cgtatcttac tgagattgcg
    29761 attgttgaag ctttaacact ggtacgctga tgagttatcc attcattata caggtcttca
    29821 aatgtcatat caggtgcttt ctttttaaca ccgtataata tttgatgtct gtactcgtca
    29881 taatcttttt gagcttcttg tttgctacta aaacctcttc tcgtagtatt tattttttca
    29941 ccagttcgtg gatcggtggc aatgtgtcct ttatacatcc acgccacttg accgtttttc
    30001 tttttgtact ttgatatttt tgccattaaa tcatctcctc ttagtttaca aggagtactt
    30061 accactagat ggtatcacct cccttcattt ttttcttagt gtattttttc ccaattgatg
    30121 aaatcgtttg ttctagcttg tttatattat cagtaatttc tctcacagct tcttcgggat
    30181 aagacatgtc tttcaattga ataggtagcc attcatctac cattctttct agtagttctt
    30241 ctatactgct agtgaggaaa agaatgttat tgtcaaattc ttctacaata gcattgatga
    30301 ataattcttc tatttttaag atattgaaaa gactaatttt ttcattttta actttattac
    30361 aagtcttgtt agcaataaat tcttgcatct ctggttctaa tttggaatat ctatccagcc
    30421 atgtatctaa attagagtgt actggtggga actgttgacg aagatgctgg aacaggtttt
    30481 tatctggaaa tgttatacca tctagtacaa tgctatctct ataaagaata attcccaaaa
    30541 tatattcttc tacattgcca caaactaact cctctatact catatttccc aaatcagcaa
    30601 ttcgggataa tcgctctggt attggtaaat aggttccctt ctcccattcg tagatagttt
    30661 ttttgtcaat taattgaccg ttgttaatca taagaccaaa tgctggttgt gttaattttt
    30721 tactctttct tatagctttt atttttgttc cgagtatctt ttttcgttct aaataattta
    30781 ttggtggacg ttttatcata ttgaccacac ctcctaaaat tagtatacct caaaaaaggg
    30841 gtgttgcaaa taaattttac aaagctgttg acaccccttt tttatcgagg tattatgtgt
    30901 atgtcgatga actggtaatc attttttgtt tgattaatcg gttaataaca aaatactttt
    30961 attttgagca aaaaatacga agataattct tagaaaattt gttcaagcaa tcacgaaagg
    31021 agggggaaat atgtctctac aaatcattga tagcattgtt tctatacaag ttgaacaaca
    31081 agtgaaagag ataattacag tgctaaagcc tatgctagaa gacattattt ctggagtcac
    31141 tctagagtat atggactatc agcaggcaag tgaatatcta ggtgtatcag taggaacgat
    31201 tagaaaatat gtttctcaat acgggttacc tgttataaaa attgatacgg tcattagatt
    31261 taaaaaatct gatattgata gtttcatgga acggtataaa ggttgagtaa acaaataaga
    31321 ctaaaacata tagtaataaa gagtttgttt ttattatctg tatgttttag tctttaacag
    31381 aattttataa ggaggaaaca caaatggaaa catcaatgat taatattcca atttatgtag
    31441 atcaagaaaa attattagag aagccagtta caaaaaaaga aaaaactgat gtaagagata
    31501 ggtgtttaga taactataga gaagttacaa gagatgagtt tgtagagata ctaaattcat
    31561 cacaatcatt tattccaagc aagacaaaga ataaaggcaa tgacaaggag agttttgtgg
    31621 aaaccagagt aattattctg gatgtcgata acactgttaa agatgagaag gggaaggtag
    31681 tagatttgag taaagatgat agtagatact taagcgtaga aaaggtttta tctatcaatt
    31741 tagttcataa ttctgcgttt gcgatacaaa aaagtattcg gtactctgaa aatctagaaa
    31801 aatacaagat tgtctttcta ttaaaagaag caattactga ctatggtaaa atggttgctg
    31861 tttacgaata cttgaaaaag aacatccccg ggtgtgatga caatgtaaat gcttcgaata
    31921 gaatgttttt tggaggatat aagtcagatg cagtgataat aattaatgaa gacaacatgt
    31981 tagatattac tgagcttccg attgatttta ccatgacatg ttctaaaaat tgtcaaagtg
    32041 attcagggag agagtatgaa ctagtcaatg aaaccgattt tgttaagttg ataaaaaatg
    32101 acgacaaaga agaaatgaaa cagtggttta aagactgcct gtttgattcc tccgacatgt
    32161 cttttaatga gatttatgaa agattgttaa ccattgatat gaataaatta cttaagtcaa
    32221 gtaaaaactt aagatgtctg tttcacaacg atcataatcc ctctgctagt atttttacca
    32281 gtaaaactgg gcattccatt ttttattgtc attccagtaa ttgtgatatc agtgaaaatt
    32341 ttattggagt tgtgatgcta attttaaaga aagacagtcg ggtagaaaca tttaattggc
    32401 tattagagaa cctagacttg tatcctacac attttcgaaa attgaaagag gaatcaaaag
    32461 tactttttga cactcttgaa tcaatgaaag acaaactttt caggacaata aggaattatc
    32521 tagaggaaat gcgacgtgtc tatgatgtct tgctttctga tgttaatttt tttgatgata
    32581 gtaaggaaag tgtgacttgt attttgtcgg gggaacaatt agctaaaaaa atgagttctt
    32641 actatggtaa aacttatgac atagataaat gtaataagtt actatcactt atgactttta
    32701 taggattgat aaaaaaatta gatgatgaag atgttccttt gaagatgctc gaatacctaa
    32761 ataggttaaa ggaagagaaa gcgttaggaa actttaatca taagagaagc aatgtctatc
    32821 gattagacgt ttttaatgca acttctgtga ttcaaaaatt gaatgaagat atccttccac
    32881 ttctggaaaa aattcatttt acatatcaat atttttctta tgaatgggtt aagatatgtt
    32941 ttaacgaaaa agaggctaaa agagtatttc ctcaaaattc ccgatcagaa ctcagcaagg
    33001 aaaaacaatt gattttagat gaagcacatg aagttttaca gggcttatcg tggcaaggga
    33061 catatctaat gacagagaaa gaacttatta acactgtttg tgaacgccta cgtaatgttg
    33121 gggataacag cgtgaaaaat gctttaataa aaaacagagg ctactttgtt aaacaaggat
    33181 tcactcttga acgagcttct aaggatatta agaaactata caatgtgaaa gcaagtagta
    33241 gttttttaat atatttagcg aaaggggcag ggatagatga aacaataaaa gaattgatat
    33301 tatcaggagc tgggatagtt cctctgtatc aatttacaag tgtaacaaga gatgatggac
    33361 ggagagtaag gaagagaaaa gacccaattg tattaaacta gacaggttat ccatagctct
    33421 taatcttatt tcatctcaat acacccaccg ttaaagttcg gtgggttatt tatttttcaa
    33481 tctttcaata gctgattgcg ttcattttct tgccttttta atagcttcat atagtcaatt
    33541 gttcgaactg catcttcagt gctaagagaa cgatagagag ccaaaaactc ttcctcataa
    33601 tctacgacaa cattgtctag aaaatggctt acaggggttc ctagggctct agaaagagct
    33661 gttaagctat cgaggttagg ctctgttaca gaattttccc atcttgctac tgcctgttgt
    33721 gtagagaaga ttttttcagc taattctttt tgtgtccacc cgagagcttt tcgcttttct
    33781 ctaatgacct ttgcatattg tttctgtttc attaatacac ccccatctca gtatagtaca
    33841 acaagttgtt gtatttgtaa tggtatttga agttttttat attttatgag ttgaaataca
    33901 tcatattgtt gtatataata aaacaggagg ggagaatcat gaaaaaagta agtaatctca
    33961 gccgatacag aaaagaaaaa ggactttctc aaacggagtt agcaaaaaaa atgaatgtta
    34021 cacagcaatg tgtaagttcg tggcaaacag ggagaacgat tcctaaaccc tatcaaatga
    34081 agatgctttc tgagatatta agtgtaccaa tgaatgagct gttttctgat gtatttaata
    34141 aagtaaacag ttaactaata agcaattgaa tgattttagg aggagaagac atgtattgta
    34201 gaaaatgtgg caaagaaata acagatgaaa gtaagtattg ccagttttgt ggagcactac
    34261 aagaaacaga tgagacaacc ggaacagaca cagtaaagaa aagtactgag cagacaatta
    34321 atgaaatgaa ggaacaaatc gtacatggac aagagacagg gaagctgtat ttgattattg
    34381 gttggatttc tatggtagta tcgttagcat ttattcctgt tgtatttggt gctgttgctg
    34441 ttattatggg ctacttatat cgagaaaaag atgagaaaat gggtacgatt ttgatgatag
    34501 caggggtagc tggagcaatc tttggtgtac ttttagggat gtcaatgtac tgataagagg
    34561 gataagagca aattattttg gagataggag ataaaaagtt gaataaagta aatatgaaca
    34621 agaagtttgt cattataggt attgttgtct tggctattat aggtattatt ttttcaaaaa
    34681 tgacaggtac atctggatta gagaagtcat caaaaccatt ggtaacacag attttacaag
    34741 aacagtacaa cttaaatcga tcatgtgatg atgtgacgat tactcatgaa aatggagata
    34801 acacataccg ggctaaagct atattagaca atggaagtgc tatcaacata aatattgagt
    34861 actatccaaa gaaagaccgt atatatgtgg agataccata tgctgaggtg ttaatgttaa
    34921 attgatttaa atgtattatg cataccgtcc aaacaggaac tcccaaaggt gtaaattcac
    34981 tagggagttt ttatagtttc ct
//
LOCUS       DAZULO010000017        34876 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000017 DAZULO010000000
VERSION     DAZULO010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.017"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..187)
                     /locus_tag="ACG8OA_000872"
     CDS             complement(59..187)
                     /locus_tag="ACG8OA_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS4975706.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACG8OA_000873"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACG8OA_000873"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS4975707.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="ACG8OA_000874"
     CDS             complement(1674..2729)
                     /locus_tag="ACG8OA_000874"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS4975708.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACG8OA_000875"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACG8OA_000875"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFS4975709.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACG8OA_000876"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACG8OA_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFS4975710.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACG8OA_000877"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACG8OA_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFS4975711.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACG8OA_000878"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACG8OA_000878"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFS4975712.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACG8OA_000879"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACG8OA_000879"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFS4975713.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="ACG8OA_000880"
     CDS             10491..12050
                     /locus_tag="ACG8OA_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFS4975714.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="ACG8OA_000881"
     CDS             complement(12161..14092)
                     /locus_tag="ACG8OA_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFS4975715.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACG8OA_000882"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACG8OA_000882"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS4975716.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="ACG8OA_000883"
     CDS             complement(15021..15773)
                     /locus_tag="ACG8OA_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4975717.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="ACG8OA_000884"
     CDS             complement(15932..16141)
                     /locus_tag="ACG8OA_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975718.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="ACG8OA_000885"
     CDS             complement(16152..17480)
                     /locus_tag="ACG8OA_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975719.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="ACG8OA_000886"
     CDS             17616..17771
                     /locus_tag="ACG8OA_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFS4975720.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACG8OA_000887"
     CDS             complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACG8OA_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFS4975721.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACG8OA_000888"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACG8OA_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFS4975722.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACG8OA_000889"
     CDS             complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACG8OA_000889"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFS4975723.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="ACG8OA_000890"
     CDS             complement(21455..22387)
                     /locus_tag="ACG8OA_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975724.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="ACG8OA_000891"
     CDS             complement(22544..23203)
                     /locus_tag="ACG8OA_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFS4975725.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACG8OA_000892"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACG8OA_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS4975726.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="ACG8OA_000893"
     CDS             complement(24244..24534)
                     /locus_tag="ACG8OA_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975727.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24943..26748
                     /gene="glmS"
                     /locus_tag="ACG8OA_000894"
     CDS             24943..26748
                     /gene="glmS"
                     /locus_tag="ACG8OA_000894"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFS4975728.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26820..28175)
                     /gene="glmM"
                     /locus_tag="ACG8OA_000895"
     CDS             complement(26820..28175)
                     /gene="glmM"
                     /locus_tag="ACG8OA_000895"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFS4975729.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28199..29368)
                     /locus_tag="ACG8OA_000896"
     CDS             complement(28199..29368)
                     /locus_tag="ACG8OA_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFS4975730.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29365..30252)
                     /gene="cdaA"
                     /locus_tag="ACG8OA_000897"
     CDS             complement(29365..30252)
                     /gene="cdaA"
                     /locus_tag="ACG8OA_000897"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFS4975731.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30441..34133)
                     /gene="nifJ"
                     /locus_tag="ACG8OA_000898"
     CDS             complement(30441..34133)
                     /gene="nifJ"
                     /locus_tag="ACG8OA_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFS4975732.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            34332..34853
                     /locus_tag="ACG8OA_000899"
     CDS             34332..34853
                     /locus_tag="ACG8OA_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFS4975733.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         9404 a         7570 c         6286 g        11616 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcctgc ttttttgaag taagcgtcat aataacccgc gcttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tcagagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 aattggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcctcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgtttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttccgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgatagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcgattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg cgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ctccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggaa attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat gtcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt taccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccggtcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatacgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaatgtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtgggg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagaggc tgggaaaacc cagcctcttc ttttttattt ctatgcttct gctactgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgccg tgaggagcca
    12301 gtaatttgat tccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcaactc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcaaagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccagcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagtaat tttttcccat acggaatgat ttcttctaca gattggaagg ttgtgttgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaac ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagctggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cctaagccat tgatctcagt ttcagtgtct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg gatccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattcaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctgcgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctacta gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcag aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgtga tcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt ggatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttcaaatc tttttataat ttaattataa
    17521 ccaatgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc gccctgaggt ggcacacttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcaagttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttaaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttctttt aacacggaac
    21661 taagcagcgc atcttcattc ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgcc actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcata ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaacgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccca
    22621 gatgttgctt tttctgatta aagactgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttgg ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagagac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaggc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgtata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttggagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt cttttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgttaatt catcttctac
    24361 cgtaaactca cgagcttgag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaaggacat ttgacaaaga atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaaagga ggatagttat accttttagg atagcgccag gtctgtgtaa
    24781 acagatgacg aggagagggt ttatcgaaag attcggcgga tggccctagg gactgcactc
    24841 tacagaaaat ccacaaaaaa tgtctgcaaa gataaaacaa aaggattttc ctgcagctgg
    24901 aagcattcat acgaattcat tatagaaagt aggatgtttt ttatgtgcgg aattgttgga
    24961 atgattggat taaaaaatgt aacaccagga ttgattgacg gtctagaaaa attagaatac
    25021 cgtggttatg attcagctgg tatctttgtc tcagatggca caactgatta tctggtcaaa
    25081 gcacaaggca gaattcaaaa tttgaaaaac aaaataaata ttgatactac aggaaatata
    25141 ggtattggtc atacacgatg ggcaactcac gggcaacctt ctgaagaaaa cgcccatcca
    25201 catacttccc aaagtggacg cttcgtgctc gttcacaacg gcgtaatcga aaactttgaa
    25261 gaacttaaaa ttgcttattt agcaaatgac cactttattg gtgagaccga tactgagatt
    25321 atcgctcatc taattgaaac tttcgcgaag gatactacaa cgcaagaggc gtttttaaaa
    25381 gctttgcggg tcatcaaagg ttcttacgct ttcgctttga ttgatcggac agcacctgat
    25441 gttatttacg tcgctaaaaa taaaagtcca ctattaatcg gactcggcga tggattcaat
    25501 gtgattgcca gcgatgcaat ggctatgctt gctcatacga aggaattcgt tgagattgaa
    25561 gatgaagaga tggttaccgt cacatcagaa aaagttatta ttcaaaattt tgcaggtgac
    25621 atcgtagaac gttcttcatt cgaagcacaa gtagatgctt cagatatcga gaaaggtacc
    25681 tatccttttt atatgctgaa agaaatcgac gaacagccta ttatcatgcg tcgtatcgct
    25741 caaaaatacg taacagaaga caatcgtgtc tctcttgatc aaaagctggt cgatacattg
    25801 tctgatagcg atcgaatcta tatcgttgct tgcggaacta gttaccatgc ggggttagca
    25861 ggtaaacaga ctttggaaaa attgacacag attcctgttg aggttcattt agccagtgaa
    25921 tttggctata acacacctct tctttcaaaa aaaccattct ttattttctt gagtcaaagt
    25981 ggcgaaacag cagacagccg ccaagtgctg gtaaaaatca accgtctcgg ctacccttca
    26041 cttacgatta caaatgttgc tggttctact ctttcgcggg aagcatcctt tacactgcta
    26101 ttgcatgctg gacccgaaat cgcagtagca tcaacgaaag cttatactgc acaaatagct
    26161 gtactaactt tattagccaa agctatcggt gataaaaaag aatttgaaac ttctcttgta
    26221 ttcgatatca ctcatgaatt gagtttagtt gctaatgcga tggaatcggt catcgcccaa
    26281 aaagattatt tagaagagtt ggcagaagta tatctcagcg atacaagaaa tgccttttac
    26341 attggacgtg gtgctgacta caacgtttct cttgaagctg ctttgaaatt gaaagaaatc
    26401 agttatatcc aggcagaagg ttttgccgct ggcgaattga aacatggaac gattgctttg
    26461 atcgaagaag gaacaccagt gatcggaatc atcagtgaag aagtgactgg tgcgcatact
    26521 cgaggaaacc tgaaagaagt cgaaagccgt ggcgctaaaa cactagttat tgtctcagag
    26581 ggattagaaa aggaaagtga ccagctggtg ctaccaactg tccaccctta tctaactact
    26641 ctaaccacag tcgtaccgac tcaattactt gcatattacg caacgctatt acggggattc
    26701 gacgttgaca aaccacgtaa tttagcgaaa tcagtgacag tagaataata aaagaacaag
    26761 gaccagtgtc agacatctga cactggtcct tgttctttta ttttttatga aaatatctct
    26821 taatccagtc cgatttcttc tttgactact gccgcgattt tatcgacata gtaattaact
    26881 ttctcatcag taggcgcttc tgccatcact cgaagtaatg gttctgtacc ggaaggacga
    26941 actaggattc ttccttctga tcccatttca gcttctgcct cttcaatcac ttttttgatt
    27001 gccggaacat ccatcgcacc gtttttatca cttacgcgga tattcactaa tttttgcgga
    27061 tagattgtta cctctgctgc aagctctgat aatttcttac ctgtctgttt catgatattc
    27121 agtaactgaa ttcctgaaag catcccatct ccagttgtgt taaaatctaa gaaaatcatg
    27181 tgcccagact gctcgccgcc aaagttataa tcgtttttgc gcatttcttc tacaacgtaa
    27241 cgatcaccaa cttgtgtaac gacatctttc aaaccgattc cttccaccgc tttgtggaat
    27301 cctaagttac tcatgacagt cgtaacgatc gtatcttttt tcaatcgatt tttttctgcc
    27361 aagtatttag cacagataaa catgatttta tcaccgtcga caatgtttcc aagttcatcc
    27421 accgcaataa tacgatcgcc atcgccatcg aaagctagtc cagcatctgc tcctttttca
    27481 acgaccatct cagctaaacg ttctggatgg gtagagccta cgccgtcatt gatatttaac
    27541 ccgtctggat ttgtgcccat tgtataaaaa tctgtttcaa gatcggcaaa caaacgatta
    27601 acacttgttg aagttgctcc gtttgctgca tcgacacaaa tggtcaaacc ggaaaggtca
    27661 ccgttgatcg tttgtacaag gaattgagaa tatttcaata agccttctgg aaattcttct
    27721 acagttccca atccttcagc tgatggacga ggcaattcat ccacttcagc atctaataat
    27781 gcttcaattt ctagctcttg gtcatctact agtttaaaac catctgcacc gaaaaatttg
    27841 attccattat cttgtgctgg attatgagaa gcagaaatca tgacccctgc gcttgctttt
    27901 tgcaaacgtg tcagataagc aacacctggt gtagagatca cacctagctg gaacacttcg
    27961 atacccaccg ataaaagtcc tgaaatcagc gcttcttcta acatttgacc tgagattctt
    28021 gtatcccgcc ctactaacac gcgtgggcgt tttccttctt cttcatgctg gcttaggaca
    28081 taacctccgc accgtcccag cttaaatgca agttctggcg taagttcctt gttggcttca
    28141 ccgcgtacgc cgtctgttcc aaaatattta cccatagttg taaaattact cctttaactt
    28201 aattttctga ggaatttgtt ctgctcattt gtgttgattc gcttgtttct gatgtttctt
    28261 ctgtacttgt ggtcgagctg cttgtcccag attccgaact ggaagaaatg gttgttgtcg
    28321 atggttccgt tcctgaatca gcagatgaaa tacttctact cgtacttgta ttttcttgtg
    28381 tccgactgct cgtcgaagca gagctactgc ttgacgtttg caacacgggt gtaatctcca
    28441 cactaactga ttcgggcttg atacttaacc cctcttctac tggtatttca attttacgag
    28501 ttgttcgatg attgatacct gagacattga tcggtacagc aatactgtca ccgatcgatg
    28561 caagcaaatc gctagagccg cttacttctg cttccagttt tgaaaaactg aaatcatagc
    28621 tttctacccc ggcagctggt gttccttgct gtatcccata aagtcgtacc gacttgcttg
    28681 gcttgatcga atcagctctc actgttactt gcactggatc agagacgata cttaatactt
    28741 cccctgctgc attcagcgcc tgtattggga cctcactatt aatcccatca tctgtgacca
    28801 tcgaagcgtc cacatcagct actactcgtt tgatttctgc caatgttttg tcacctgtag
    28861 tgatagagac ttttttagga ctaacggaag tatcatcaag ttcatatcca gatggtgtga
    28921 acgttgaagg aatcaacgtt tccacatcga aatcttttat tacctttttc tcaaccgtta
    28981 ctgtaatagt cgatggttcg atctttgctg tgacagcact gctcaaattt tgaattttta
    29041 gcggaacttc atgtgttcct tcgcccagct tagtgatatc tgctgtgacg cggaacatcc
    29101 gtgtatcttc gtttgcctct gcattcaact gaactcggtt tgcactggaa agtttgacag
    29161 tcactgcatt ttcatatcca tggatatagt agcgctttga atcataagaa atcttgatcg
    29221 gcacccccgt aactgtttga ttatacgctt catttccaga aagcgtattt ggaacggact
    29281 ggccatttgc attgaagtac agcgcaatac tgaacagcaa tgcaataaaa ctgtaaagaa
    29341 tgcttgtccg tttctctttt gagatcattt gcgatcccct cccttagtca taccgtctag
    29401 gaaagattga atcacgtttt tcttgttttt tgttttctct tcagcaatta gttctctacg
    29461 caagattgcc aaatattctt gctgggataa gccagccatc agttcattat ctaacgtgat
    29521 gctgacgtcc ccggtttctt cagagacaac gatggtaatc gcgtcactga cttcgcttat
    29581 accgacagct gcccggtgtc gagtcccgaa ttctttaggg atcaacatac tttctgatag
    29641 aggaagatag gcgcttgcta ctgcgatctt tccttctttt acaataactg caccgtcatg
    29701 cagcggtgta ttaggaataa agatattgat cagcagctcc ccagtgatat ctgcatctaa
    29761 agcaatccct gtctcaatat actcatctaa tccagtatga cgttcgatag tgatcaacgc
    29821 accgattttt cgcttggaca tatattggat ggctttgtca aaagacaaga tcatcttctc
    29881 atcttcctgc tgttcgcttt ttgattgttt gaaaagcgaa ctgcgtccaa gatgttccag
    29941 tcctctccgg atttccggct gaaaaatcac gatagcagct atcaccccgt acgtaatgac
    30001 ttggttcatc agccaagaca aggtgtgcag accaatgatt tccgctacga ttcggataac
    30061 aataaaaata gctacacctt tcagaagctg aatcgctttt gtgcctcgca gcagttggat
    30121 cagtttgtat acaagatacc agacaaccag aatatcaagt atgttcacca aaaaattcag
    30181 agagaacaag tcaccggaaa acatttgctg ccaatagtcc aaattaaaaa gttccccgat
    30241 ttgaaatgac ataaaaagcc tctcttctct tttgagattt cctctctagt atatcatatg
    30301 ttttaatttt agaggctgat atgctcgtat ttttcttgag aaaaacgcat aattttcaag
    30361 aaaattgcaa gtatgcgttt tatttttctc tgcaataaat atttttaaac ttcagagaat
    30421 tattcattat attttttctg ttatttttcc tttttttctt ttgtagcttg ttccattttt
    30481 ttacgtcgtt ctgctctggc agctcttcta gcagctcttc tttcttctgc ttcaaggtcc
    30541 gtcgtttttt cttttctagt cttaacgggt acatcgatag attcctctgg ttcaagtcgt
    30601 ttcttgattt tttctaattc tcccgtcagt gcagcatagt tgtaaaaacg tgttttagcg
    30661 tcgtggacag tcttttcaaa taaagctttt gcaagatttg gatttgcgtt atctagcgag
    30721 gaaaagcgaa cttgtttcct catgaaatca ggcatagcag agaaatcagg ttttttgtag
    30781 tccaaagtca ttgggttctt gtttttttcg accattgtag ggttatatcg atataatgac
    30841 caataaccag aagaaacagc ttcttttgct tctttaagcg attctttcat tccgccaagc
    30901 aagccatgtg tgatacaagg cgtataggca ataatcagag aaggaccagg atacttttct
    30961 gcttcttcaa atgccttgat cgtttgcatt tgattggcac ctgaagcaat ttgcgccaca
    31021 taaacatttc catatgtcat cgccatcatt cctagatctt tctttgaagc atactttcca
    31081 ctagccgaaa atttagcgat tgccgaagct ggtgttgctt ttgaggtttg accgccagta
    31141 ttggaataga cttcgttatc caaaacaagg atattcacgt ctgctccgct ggcaagtaca
    31201 tgatcgatcc cgccaaaacc aatgtcgtaa gcccatccgt ctccaccaat catccattgg
    31261 ctgtttttta cgaatagatc ctttctcttg tatatatcct ccagttcagg gaaaattgat
    31321 cgttcgcttt cgatcgcagc ttgcagtttt gctgctcttt gctgcgttcc ttcactctca
    31381 aacatgtgta ctaaccagtc ttccatcaat tctcgtagtt cttctgatac ttttggcaat
    31441 acttctttga ttgaacttgc caaagcctcg cgacgagtct gtgccgctag aagcatacca
    31501 taaccaaatt cagcattatc ttccaataaa gaatttgacc atgcaggccc ttgaccacat
    31561 tcattggtcg tataaggaga agcaggagaa gcaccgcccc aaatagaaga acagcctgtc
    31621 gcattcgcga tcatcattcg atcaccgaac atttgtgtca ataattttac ataaggtgtt
    31681 tcgccacagc ctgagcaagc gcccgagaat tcaagaagcg gttgattgaa ttgtgtagac
    31741 aagacagtat tctttccttt gactggattt gctttttgtc ttaatgtcat tgcaaaagcc
    31801 caattcacag cttgttcttt ctgttcttca taaggcttca tcactaaagc tttttctttt
    31861 gcgggacaag cctctacaca cagaccacaa cctgtacagt cttccactga aacttggata
    31921 cggtatttta cgccgtctgc gcctcgcatt tctctcacga taaatccttc tggcgcttct
    31981 tccatttcct cttcatctgc taaaaatgga cggatggcag catgaggaca aacaaaggca
    32041 cattcattac acatcgtaca gcgatcacta atccactcag gtacttctaa agcgacgccc
    32101 cgcttttcca tagaagccgt tcctaaaggc atttcgccag ttgtcattcc atttgctaca
    32161 agtgtaccaa cagagagttg atttccttcc tgtgcattca ctggttcaag tatttcttgt
    32221 atataaccag tgttcctttt attgattttt tcaggcatct ccaacgttct ccaatggtct
    32281 gggatcgcga cttgatgaag cagttcggtc gtccgatcga ttgcttgtat gtttttttct
    32341 acgatagcca ttgatttatg tccatagctt ttcagggctt cttcttttag cagcggaagc
    32401 acctcatcaa aaggaatgat gtcagctaat ttaaaaaaag ccgtttccat tgccgtattg
    32461 atccggcgtc ccaaaccgac ttcttgggcc aatctcatgg cattgatcgt atagaatcta
    32521 atgttatttt cagccaagta acgttttaat tttaagggca gatgcgtttc taattgttcg
    32581 tcgctccaaa gagtattcaa taaaaaaatg ccacctggct tcaatccttt gaccaagtca
    32641 tagctatgca ggtaagctgg tgtgtgacag gcaacaaggt ccgcatgctc gacaagatac
    32701 gctgaacgta tcggcgtatc tccaaaacga agatgagaga cagtcagacc gccagatttt
    32761 ttcgaatcat aataaaaata gccttgcgca tatttatcgg tgtgatctcc aataatcttg
    32821 atagctgatt tatttgctcc aactgttccg tcagatccaa atccccaaaa cttggcctgg
    32881 aatgtttgcg gttctgtcaa atccagactt tcctttggtt ccaaagattg ataagtcaca
    32941 tcatccacga tccctatggt aaaccgtttt ttgcgtatgc ttgggtcttt tttcaattca
    33001 tcaaaaactg ctgagatttg gtcgggagtc acatctttag accccaatcc gtagcgacca
    33061 ccaataacag ccgggcgaag ttcagaatca tataacgcac tttgaacgtc taataagaga
    33121 ggttctccac ctgcccctgg ttctttactt cgatcaagaa cagcaattgt ttttactgta
    33181 tgaggcaact tttgcacgaa tgtttccact ggaaaaggac gataaagatg gatattcaaa
    33241 aaacctgttt tccgtccgtt tgcattcaag taatcaatcg tttgttcaat cgtctgtgca
    33301 actgatccca tagaaacaat gacttcttcc gcatcgtctg ctccgtaata agtcaccaag
    33361 tcataagaag ttcctcgtaa tcggttgatc tctttcatat atttttgaac gatttcaggt
    33421 aatttatcat aataggggtt gatcgtttcc cgttgttgaa aatggatgtc tggattctgg
    33481 ttcgtaccac taacagtcgg atgattggga ttcatccctc gtttacgaaa tgaagctaat
    33541 ttctcctgat tgatcagagg agctaattct tcatattcca aaacttcgat tttttgaatc
    33601 tcgtgactcg tacggaatcc atcaaagaaa ttcaaaaaag ggacttctcc ttcaatagaa
    33661 gcaagatggg caacaggtgc taaatccatc acttcttgta cactgctttc agctaacata
    33721 gcaaatcctg tttgtcttgc agccatcacg tctccgtgat ctccgaaaat attcaacgca
    33781 tttgtcgtca ctgcccgact agcaacgtgg aaaacacttg gcagtagttc cccagcaatc
    33841 ttgtacatat tcggtatcat caaaagcagc ccttgtgaag cggtatatgt agttgtcagt
    33901 gcaccagttt tcaatgatcc atggaccaca ccagctgctc ctgcttcgga ttgcatttct
    33961 actactttta ctggttgacc gaatacgttt tcttttcctg ctgctgacca ttgatctact
    34021 aattcagcca tggtcgaact gggtgttatt ggatatattg cagcaatctc ggtaaatgca
    34081 taagaaatat atgcggctgc cgtattgcca tccattgttt tcatttttcg catgattcat
    34141 catccttgtt catattctct cttattatct gttccattat aacgtaaaac atgccatttt
    34201 tttcttttat ttataaaaat attttacatg tatctatttg aattggtcat ctaattttta
    34261 gacaaacagt ctaaatcgtt gccttttttt gtcagacatg tataaactat aagcgaaaga
    34321 ggtgttttaa catggatata tcagtgattg atgcaacgaa aacaaataca caaaaaggaa
    34381 tcctttatgg ttcatcgaat gctcctaaaa aaatggtcga attcatcaac ttagcttgtc
    34441 cgtattgccg acaatggttt gaagaatctt atgagctatt agaagaagca gtccaaagcg
    34501 ggcaattgca gcgagtgatc aaattgttcg ataaagaaaa agaaagctta cagcgaggga
    34561 acgtgatgca ccggtatctc acaatcagcg atggccaaaa agcgatcaaa gagatcaaac
    34621 aaatattcga tactcaagac gagtggaaac atctttctct ccaagaagta gcggattttg
    34681 cagtagataa actaaagctg acagaacaaa aagacgaaca gctgagccaa gctgtcatca
    34741 acgaagctga gcaagcacat attcgatttg ttccaactgt catcttgggt aaagaaatct
    34801 ttgatgaatc tatttcaata gaggaattaa aagaattgat ccaaacaaaa taaaaaaata
    34861 aggagcgtgg gacaaa
//
LOCUS       DAZULO010000018        34330 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000018 DAZULO010000000
VERSION     DAZULO010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.018"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            202..1077
                     /locus_tag="ACG8OA_000900"
     CDS             202..1077
                     /locus_tag="ACG8OA_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFS4975734.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="ACG8OA_000901"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="ACG8OA_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="ACG8OA_000902"
     CDS             complement(1642..2973)
                     /locus_tag="ACG8OA_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4975735.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="ACG8OA_000903"
     CDS             complement(3122..3835)
                     /locus_tag="ACG8OA_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4975736.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="ACG8OA_000904"
     CDS             4010..5434
                     /locus_tag="ACG8OA_000904"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4975737.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="ACG8OA_000905"
     CDS             5733..6440
                     /locus_tag="ACG8OA_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS4975738.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="ACG8OA_000906"
     CDS             6427..6750
                     /locus_tag="ACG8OA_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS4975739.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="ACG8OA_000907"
     CDS             complement(6839..7876)
                     /locus_tag="ACG8OA_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4975740.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="ACG8OA_000908"
     CDS             complement(8197..9180)
                     /locus_tag="ACG8OA_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFS4975741.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="ACG8OA_000909"
     CDS             complement(9595..10365)
                     /locus_tag="ACG8OA_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4975742.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="ACG8OA_000910"
     CDS             complement(10358..11293)
                     /locus_tag="ACG8OA_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4975743.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="ACG8OA_000911"
     CDS             complement(11609..13258)
                     /locus_tag="ACG8OA_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS4975744.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="ACG8OA_000912"
     CDS             complement(13375..15978)
                     /locus_tag="ACG8OA_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFS4975745.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="ACG8OA_000913"
     CDS             complement(15990..17039)
                     /locus_tag="ACG8OA_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS4975746.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="ACG8OA_000914"
     CDS             complement(17065..17379)
                     /locus_tag="ACG8OA_000914"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFS4975747.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="ACG8OA_000915"
     CDS             complement(17395..17841)
                     /locus_tag="ACG8OA_000915"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFS4975748.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17842..19764)
                     /locus_tag="ACG8OA_000916"
     CDS             complement(17842..19764)
                     /locus_tag="ACG8OA_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4975749.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(19976..20872)
                     /locus_tag="ACG8OA_000917"
     CDS             complement(19976..20872)
                     /locus_tag="ACG8OA_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4975750.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(20865..21545)
                     /locus_tag="ACG8OA_000918"
     CDS             complement(20865..21545)
                     /locus_tag="ACG8OA_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4975751.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(21546..22328)
                     /locus_tag="ACG8OA_000919"
     CDS             complement(21546..22328)
                     /locus_tag="ACG8OA_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4975752.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(22321..23238)
                     /locus_tag="ACG8OA_000920"
     CDS             complement(22321..23238)
                     /locus_tag="ACG8OA_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4975753.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(23402..23656)
                     /locus_tag="ACG8OA_000921"
     CDS             complement(23402..23656)
                     /locus_tag="ACG8OA_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975754.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
     gene            24513..27740
                     /gene="fss3"
                     /locus_tag="ACG8OA_000922"
     CDS             24513..27740
                     /gene="fss3"
                     /locus_tag="ACG8OA_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFS4975755.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27853..28167
                     /locus_tag="ACG8OA_000923"
     CDS             27853..28167
                     /locus_tag="ACG8OA_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4975756.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            28180..28554
                     /locus_tag="ACG8OA_000924"
     CDS             28180..28554
                     /locus_tag="ACG8OA_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4975757.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            28564..28899
                     /locus_tag="ACG8OA_000925"
     CDS             28564..28899
                     /locus_tag="ACG8OA_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFS4975758.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            28982..30322
                     /locus_tag="ACG8OA_000926"
     CDS             28982..30322
                     /locus_tag="ACG8OA_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS4975759.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            30400..31074
                     /locus_tag="ACG8OA_000927"
     CDS             30400..31074
                     /locus_tag="ACG8OA_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS4975760.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            31067..31231
                     /locus_tag="ACG8OA_000928"
     CDS             31067..31231
                     /locus_tag="ACG8OA_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975761.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            31307..32449
                     /gene="dcm"
                     /locus_tag="ACG8OA_000929"
                     /pseudo
     CDS             31307..32449
                     /gene="dcm"
                     /locus_tag="ACG8OA_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            32439..32729
                     /locus_tag="ACG8OA_000930"
     CDS             32439..32729
                     /locus_tag="ACG8OA_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975762.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            32988..34172
                     /gene="mobT"
                     /locus_tag="ACG8OA_000931"
     CDS             32988..34172
                     /gene="mobT"
                     /locus_tag="ACG8OA_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS4975763.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            34169..34306
                     /locus_tag="ACG8OA_000932"
     CDS             34169..34306
                     /locus_tag="ACG8OA_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFS4975764.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT        10701 a         6609 c         6254 g        10766 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt
    17941 ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga
    18001 atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg
    18061 cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga
    18121 tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac
    18181 caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa
    18241 aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg
    18301 tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac
    18361 aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct
    18421 atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt
    18481 atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac
    18541 tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc
    18601 gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac
    18661 acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat
    18721 atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta gattattgat
    18781 caattcttca tcaggataaa attcatttaa tttaattctt actaactctt gtagctcaac
    18841 acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag catttttttc
    18901 aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa taattgaaaa
    18961 tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt cttcagaatt
    19021 attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag caaggtggat
    19081 agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca tgtatcgatt
    19141 aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg cttcaccaat
    19201 gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa agccacccca
    19261 ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag ctttgatgcg
    19321 cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt ttctaacatc
    19381 tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa gattttgaat
    19441 ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac ggtcctccgg
    19501 agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt ctttttttcc
    19561 aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca ttttttggat
    19621 tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat ataattgttc
    19681 tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg
    19741 ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta agtgtacaat
    19801 tattatctct attttcatac agaagaaagt tcttgattaa tgtagaagtt tctaatattc
    19861 ttgattattt caaatcaata aaaaagcctg caatatttat atcacaggct tttttctcta
    19921 ttaaatatat ataagataat acatttttct actagttctc actaatcttg tataattaaa
    19981 ccctccgcaa cttgacggag atggagaaga tgcccccgtc taatctagcg gtcatctctc
    20041 ctcccattcc ttccaccaat ttttttgaca ggtaaagccc gattccgcta gattccgtat
    20101 ttgtgcggct ggtattttcg gtataaaaac gctggaagac tttttcaaca gaggtttgcg
    20161 ttcctggttt gataggattg gccactgtta atcgaatgaa tggatcctct tttgaaaatg
    20221 tcacctttgc ttgtccatct gcataacgca ataaattctg tatgatattt tgcagcactc
    20281 gcagcaacat gagcggatct gctaatacag ttgtatttat cgagtgttca gcgaacgcta
    20341 cctcgatatt tttctcagaa aattgttcat aaaaagagaa aaatacttct tccacctctt
    20401 tagaaaaaga aagcgtatgg agatccattt ctttttgctt cgtctctatt cgagcaagat
    20461 cataaaactg atccgtcata ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat
    20521 acttctcccg tttctccgta tcctctacct gttccagtaa ttgtacataa ccgttaatag
    20581 cagtcaaagg agttctgaga tcatgagaaa ggccgacgat cgatagtctg atgttttctt
    20641 ccatttcttg aaatgcttgt cctttttgtt cattttcatc caaccatgta ttcagtgcat
    20701 ctactaggtc cattagattc ttttctcgaa attcgactga aagacgacca ccatattctt
    20761 gtcgttcaga aagagtggcg attttcttag ttaatctctt gatttccctt tgcaggaaga
    20821 aaataaaact tcccataaag ataacaacga gaagcaacag ccatttaatc aaatctaaac
    20881 cccactcccc acaccgtatg aatatacaca gcatgtgcct ttgctagctt ccctcgaaga
    20941 ttggaaatat gcatattcac tgttttctca ctatcaaaat atggttcttg ccaaactgtt
    21001 tcataaagat ttgctcgcga aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga
    21061 tcgaattctc ttcctgtcaa atggacagcc acaccattca catttacatc cctcgactct
    21121 ttgtccagta agatttcctt gtaatagatg cgttgcgaca gatcttttac ttggacagaa
    21181 gtctgatgac gaagttggat gccgattcgt aacaaaagtt cttcgatatc aaatggtttc
    21241 gtcacatagt catccgctcc tgctgccaac aaggaaactt tatctctttg atcgcttttt
    21301 gctgaaagaa cgatgatcgg aacattcgat gtctgtcgga tttctgctaa caatgcttca
    21361 cctgaaagcc ctggaagcat gagatctaga aggatcaaat cgacaggata tgtgcttaac
    21421 aataattttc cttccgtacc tgaataagca tcaagaagtt tgtatctctg ttcaagaacc
    21481 tcctccagta aggaatgaac agcttcatcg tcttcgataa tcaaaatagt ttccagcagg
    21541 accacctact taatgtcaga tttttggaaa ataaacattc ctagagctga tgataccacg
    21601 attgttacta gactgaccag aatcattttc ggtgcttgcg aataagttgc ttgttgcgca
    21661 aagtccgtat acatgagcca cgtttccgta aaccactccg ttagaaattc caagcctttg
    21721 atttttgcga aaacaccggg aatgaaggcc gttcccatga tataaagaaa agaaagaagc
    21781 aaactgacac tggtacttct agtgatgaat gtcaaagcca ctggaacaga taatgtccct
    21841 aatgtcagca gaagcaatac aggaaatact tttagaacat aatttccata gtttttgatt
    21901 ccctcaactt caccaaatcc gaaagcaagt gtgtaagcaa gcatggctag tcccgtgatc
    21961 aaaaccacgc caagtgttgt gatcaaaagc gtaacgagaa gtttcgacac ataataagtc
    22021 attcgattcc gtcctaaaga taacgtattg cgaatcgtac cgttttgaaa ttcttcccca
    22081 atataaaagc acgcaaatgc tgcgaaagcc agaagaatcc atgcagccat tttttctacc
    22141 attaaaatcc ctgttgcacc gttgatgact gtatccgttg cttggtttaa ttcattgccg
    22201 tattttgtat gcatatacca ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt
    22261 cctagtgaga cccacatttt acggctcttc catactttta ttttttcagc agttaataga
    22321 ttaaacatgt ggattgcctc cagttaattg taagaaataa tcttccagtt tttgcccaac
    22381 taggtgaata ccatccacag gaacttttgc ttctaccaat gcttgattga tggctgctac
    22441 ttggtcaatt ccttcaaaaa gctggataac acggctagat tgtacaaaat aatccctata
    22501 accaagagag tcgagaaccg ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa
    22561 ttgacgagat tcttgtgcca attcttcttt cgataattct ttgatcaagt gtccttcatg
    22621 caaaatacca tagtgagtgg cgatctgtga caattcatct agcaagtggc tagaaagcaa
    22681 gacggtgatg ccccgttctt ttaccaaacg ttggatgatt tctcgcattt ctgccatccc
    22741 tgctggatcc atcccattcg tcggttcatc caaaatcaaa aattctggat tcgtgatcag
    22801 tgcatttgca atagctaaac gctggcgcat acctaaagag aaattctttg ctttcttttt
    22861 ccctgtattt tccaatcgca tcattttcag caggtcttcg atttctctat ccgacacacc
    22921 gccgttcgct gcttggatac gtaggttatc tcttgcagtt aattctggat aaagagcagg
    22981 ggtttcaatc gattgaccca tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa
    23041 ttcgatcgaa ccttcatcaa tcgagatcaa acccataata gttctcataa aggtagactt
    23101 acccgctcca ttttccccaa tcaatccata gatcattcca cgtttgattt cgattgatac
    23161 tttatctaat gctttggcag caccgtattt tttggttata ccagttgctt tcaagaccgt
    23221 ttcatttcca aaatccatac ttatccttct ttcttattga tgagtgaagt atagttgacg
    23281 ggtataaaga aagcagccag aaatgtataa agaaagtgta aattttccaa ttgaatgtca
    23341 aaaaaaacaa tcggacaata cgtgtctttc ctattatcag gcagtttgtt atacgcttgt
    23401 atcagtttcc tgattctttc ttttcgacca aagtcgtttc tctgtattcc tcttcactga
    23461 aaatttttgc ttctttcaga tcaagcttga gtaattttac cagaccggtt ataaggataa
    23521 ctatagcaag actccctccc agaaaaatta gactgtctcg cagacgtggt tgcgttgtct
    23581 tccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc atggtaatcg
    23641 ttgttatcgt tgacataagt cgtccttgta tatgcgttgg aacttctcgc tgaagccaga
    23701 ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa aaataataca
    23761 tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag acttttcaca
    23821 atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    23881 ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag ctgaccactt
    23941 ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat ctttttcccg
    24001 tttgtttata gatcgaatga tattatagac caagaatcct acctattaac atagctaaaa
    24061 acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga ttaaggtaac
    24121 tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg aacgataatc
    24181 gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct ggttttttct
    24241 tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg taaatatatt
    24301 ttattatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta atttaaaggc
    24361 agtatatttc gtattttaga ttaaattttt aggctcattc attctcaaat ccattctatt
    24421 cagtcaataa aaattttaaa aagactttat aaacaaagat gttcaagcga gcatcttatt
    24481 tttttgtgct taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa aagaaattat
    24541 gtgtattaac tattttgagt ttggctgttc aagcaattgt tggagcaatt gctcctacga
    24601 ttacttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta gatttagcac
    24661 atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa gtcactgttc
    24721 cgcattatgc agtgtacaac ggctttaaac aagatatttt ctgtattgaa ccaggagtcc
    24781 cgatttacaa tgagttcact cctggctatg agaaaaaccc gttaccagat atgtccgata
    24841 aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat actcatattg
    24901 tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca ataaaacgat
    24961 cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa gccattgacg
    25021 attatcagaa gaaaccaacc ttccataata gtacggctaa aacagtatta gggcaatcga
    25081 ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg
    25141 ccgcaaacat tgattatcgt gtgaatggta atcagttgat aatcacccca aatgccaatt
    25201 caaaagaaag tggtgtgctg acgcttaaga aatctgctgg taccggaaca cctgtcgcct
    25261 ataaaaaggc tggccaacag actttaatgg ccggggcgat tgataaacct aatacctaca
    25321 caatcaaaat tgatgtggaa acggagggtt ctttaaagat caaaaaagtc gataaagaat
    25381 cgggtgatgt tgtaccagga acggttttcc atttagactt tggaaaaact ttacctgcaa
    25441 aagacgtgac tactgacaaa gaaggcattg ctacattgga tgagattcct catggtacaa
    25501 aagtaaccat taccgaaaaa tcagtaccgg cgccttatac gattgatacc acccccatga
    25561 ctaccaccat taaagcaggc gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag
    25621 ggcaaatcat tctggataaa agcggagttg aaacagggag tgatctttgg aatgacaatt
    25681 attccttagc tggtaatacg tttgccattc gcaaagacag tccaaccggt gaaatcgttc
    25741 aagaaatgac cacggatgaa aacggtcatg cggaaacacc aaaagagatt gctaatgcgt
    25801 tggaattggg aacctattat gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt
    25861 tcaaacctgt aaaagtcgaa ttgaagtatg ccaatcaaac cgtagcccct gttaccagta
    25921 acgtaaaagg gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag
    25981 atactgggga taaagcacaa ggtaaggcag tctttgaggg tgctgaatac accttgttta
    26041 ctgctaagga tggaaaagct gttaaatgga gtgaggcttt taaaccagag ttagcgaagg
    26101 gaacgaaagg ttctgatgaa acggtcactt tgactttaga cgaaaagaac caagttgccg
    26161 ttaaacattt agctatcaac gagtattatt ggcaagaaac aaaagcgccc gaaggatata
    26221 gccttgatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg
    26281 ccgtgattac ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc tttgatttct
    26341 ttaaatttgc tggatcagct gcgggcacta ccgaaactgg cttcaatgat ctgaccttta
    26401 aagtgtcgcc attggaaggg accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag
    26461 cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt ccttatgggg
    26521 attatttact tgaagaagta gaagccccag aaggttttca aaagattacg ccattagaaa
    26581 tccgttctac ttttaaggaa aacaaagaag actttgtgaa gagtgagtat gtctttacca
    26641 ttaccgaaca agaccaaaaa caaccaatta aaacggtgac cgttccttac gagaagctga
    26701 cgaacaaagc attttctgtt agtttgaacc gtttaatgct ctatgatttg cctgaggaag
    26761 aagatagttt aacttctctt gcgacttgga aagacggaaa caaagaatta acttctctgg
    26821 attctaccga gctagttgat aaattgagtt acaatctaca tgaaatcaaa gaagactggt
    26881 atgtggtagc gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa
    26941 aagccaaacc agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag
    27001 gaacttggaa aattcagcat aaattaaccg ccgaacaagt tttaaataaa actattgtct
    27061 tattcaacta tgtgtatgaa aataaggaag cttttgaagc aggcgataag ccagtagcga
    27121 aggatgttag cttgaataac caagcccaaa ctgtcagttg tacagtggaa caccatgttt
    27181 ctatccaaac aaaggcccac ctagaaaacg gttctcagac ctttactcat ggtgatgtgg
    27241 tggatatgtt tgatgatgtg tcgattactc atgatgtact ggatggctca aaagaagctt
    27301 tcgaaacaat tctgtatgct ttattaccgg atggtacaaa caaagaaatt tggaaatctg
    27361 gcaagattga ttatgaagta aatgataaag aattcaccaa aactgtgctt gcgaaaaaag
    27421 tagataccgg aaaatatcca gaaggaacca agtttacttt tgcggaaatc aattacgata
    27481 aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct caaaccttaa
    27541 caccaaaaga agttccaacc atactaagta cgccgaaaca accggaaaca ccaactgttc
    27601 caagtggctc tcaagaatct agtcccacag tgaagacctt ccctcaaacc ggggagaaaa
    27661 attccaatgt tttactgttc attggtttta ctttgatttt tgcgacggcg ggctattatt
    27721 tctggaatcg ccggaactaa ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg
    27781 cgcgaccgcc tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa
    27841 gaggaaaaga agatggaatt gaaatttgtc gtgccagata tggctgaaac ctttggaaag
    27901 ttaagttatg ccggtgaagg agaaattata acagaaggat atggacggaa tactacggtg
    27961 attggtcgca gttatcattt gtattccagt aagcaacggg ccgatgatat tgaggtggta
    28021 gtagctgcgg aagccggtga aaaggatttt gatcaagacc aaccgctaaa agccgtgaat
    28081 ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttactga ttacgtgttg
    28141 tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac agaaggtatt
    28201 gtcgtagatt caggtttaac atttgggaag ttgcgttttt ccgcattacg ccgagaagta
    28261 cggaagcaaa atgaggatgg aacaattggt aatgaggtaa aagaacgaac ctataattta
    28321 aaatcctctg cacaagggcg aatgattcaa gttagtatcc cagctaatgt acccttacgc
    28381 gagtttgctt atgatgcaga ggtagagttg gtcaatccaa tcgtggatac cgttgccaat
    28441 tatgtttttc gagaaggaac taccgttaat tggttcatta aggcggatga tttagtcttg
    28501 aagcggcagc cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag
    28561 attatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg aaaaggcgag
    28621 ctgactgagg aacaactcgt taaagcatta acctctgatg aaaaaatgtt tctgattata
    28681 catcaaggtt tacttggaga aaatagcgac agtgaagaag actctgatgt actagactgg
    28741 ttaggagaag aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag
    28801 gcagaatcca ttttggaaga aattggtgtg gagatgccgg atgctattga aggcttttta
    28861 aatcaattgg ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac tagcataact
    28921 ttttgaaatt gcctaatgaa atcctattgg gctttttcga ttggaggtgg aaaccatcaa
    28981 aatgtacaaa ggacatcgca tacgcgcagg agatcagcac ttggtttatc actttgttct
    29041 tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc aagagtttag
    29101 acaatttgat atttctaagc tatcactttc tactattgaa atcgtttggt ctataaaaga
    29161 tttagtatgt ctgcttggaa gtctcgcttt ttcaggagct atgatattgc tttatattca
    29221 cttttttctt gatcattggc gaagcttgtg gcatcgacaa aagctagctc ggatgattct
    29281 agagaaccat tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa
    29341 tagtagccga accagagaga ctatcagtta cttccccaaa atctgttatc gaatgaaaga
    29401 tggattactg tctattcgcg ttcaaatttc acttgggaaa taccaagatc agcttttaaa
    29461 attggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg aactcaaaga
    29521 ctcctatgtg gagtatactt tactgtatga tatgatcgcc aatcgaattg ggatagacga
    29581 agtagtggca gaaaatggga ctctgcgatt gatgaaaaat caagtatggg cttatgattc
    29641 tttaccccat atgttaatcg ctggtggcac gggtggaggg aagacctatt tcctcctcac
    29701 cattatcgaa gcactattga agtcagatgc agaattgttt atcctcgatc ccaaaaatgc
    29761 agatttagct gatttaggta cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc
    29821 tgcttgtgtg gaagatttct atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat
    29881 gtctaactac aaaacaggag agaattatgc ctatcttgga cttccaccca actttttaat
    29941 ctttgatgaa tacgtggctt atatggagat gttaacgacg aaagaaagtg cggtgatttt
    30001 gaataagctc aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc tgattttggc
    30061 ttgccaaagg ccggatgcga aatatttagg agacggaatc cgcgatcaat tcaactttcg
    30121 cgtggctttg ggtcgtatga gtgaactcgg atattccatg atgtttgggg aggtcgaaaa
    30181 aaacttcttt atgaagcaca tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat
    30241 tagtgagttt tatacgccac ttgtaccaaa agggtatgat ttcttagatt caattagtaa
    30301 attagaaaag atgaatgatt gagtgatctt caatactgac tttatacttc aagtaacgta
    30361 taatactttt atatagtagg aagaaagggg ataattaata tggaaagtaa tgattcttat
    30421 ttagagtatg caaaagcaaa taaggataag tttatttctt atattatcaa agggaaatcc
    30481 cccgaagttg gacgagacgc agtttttatg gcagggagtc ctggggctgg aaaatcagag
    30541 gttgccattg gattagctga gaattatgat aaccatgtga ttattgatgc ggattacttt
    30601 cgcacccagt ttcctgatta taatggaaaa aactctagtc tctttcaaaa agcatcttct
    30661 tggttggtag agcaggcatt tagatatgtc ttagatcatg gctattcttt tattctggat
    30721 gcaacttttg caatattaag tgctgaaaaa aatatctcgc gggcgttgaa aaaacatttt
    30781 cgagtgacga ttttctatgt ttatcaagac cctaaaatcg cttggaaatt tacacaagaa
    30841 cgtgaaattg cagagggacg tcatgtgccg aaaaaggcat ttattaacgc ttttttcaaa
    30901 tcaagagaga acattgcaaa agtaaagagt aaacatccag atgtattgct tcatatcata
    30961 ataaaagatt atacgaataa tatttccgaa gttcattatg ctacggataa tctagaatta
    31021 tttttaccac tagggtatag taggaaagat ttggaggttc aattacatga ctgagaaaga
    31081 acaagtaacg aaaattgtaa agaaatacaa taagagcatt gcggatttat cggaaaatgc
    31141 tactgcaaaa gagttcaaaa cggtgattaa atatgtagcc gatcaggcca atgaaaaaca
    31201 acggaaatta gttggattaa ataaaaaata gtaggacaaa ttaataatgt atgagccaag
    31261 agcaaaatgt tcttggcttt tttctttgca atgaatgagg taatgtatga gatttttaga
    31321 tttattcgcc ggtattggtg gctttcgttt aggtatggaa caggcgagcc atcattgtat
    31381 cggtttttgt gaaattgata aatttgctag acgtagttat aaagcaattc atgatacaag
    31441 taaggaggtg gaaatgcatg acatcacaag tgtatcagat gagtttattc aatccctcgg
    31501 accagtggac atcctttgcg gcggatttcc gtgccaagct ttttcaattg cgggaaagcg
    31561 gcaaagattt tccgatactc gaggtacttt attctttgaa atcgctcggt tcaccactct
    31621 tctccgacct aagcttttat tccttgagaa cgtccgagga ttgctcaatc acgaaggagg
    31681 ggctacgttc gagacgatcc tccgaacgct ggatggattg gggtatgacg tggagtggta
    31741 ggtgcttaac tcaaaggcct acattcccca aagccgggag cggatattcc ttatcggaca
    31801 ttctcgagac gcatgtaccg aacaagtatt tcctatcatt ggaccgtctt caacatctga
    31861 tcaaaacatc agaaatttgg taaatatcaa tccttccaat cgaggaatgg gtggacaagt
    31921 ctatggttca gatggcgtag cgccaacctt aacaggtgat gaaggaatca aaatcgcact
    31981 gccagcaagg gatggaatct ctgtagcagg actattaccg ggaaagtttg aacaaggtaa
    32041 tcgagtttat gaagttactg gaaccgcccc caccttgtca acgaaacaag gtggtacaaa
    32101 aatcatgatt cgaaaaaatg aaacagaata tcaagaggtg aagccagaag acagtgtgaa
    32161 tctggctttt cctaattcca caagtcgccg aggtcgtctg ggaaaacaga gtgttcatac
    32221 cttactaaca ggagaccaac aagcgatcgt cacggatcag tatcaaattc ggaagctaac
    32281 acctagagag tgttggcgac ttcagggctt tcccgattgg gcttttgatc gagattctca
    32341 agtcaattca gatagtcagt tgtataagca agccggaaac tctgtaacag ttccggtcat
    32401 ttttgatatt gccagaagat taaaagagtt ggatgtacat ggtttatgat gagttgattg
    32461 gagaaattta ttgggtgatt ggaaagattc agtcagaccc agaattagaa gaacaattgc
    32521 gacggttaaa atttgatatt cgcaagaatg gtgtcaaagt cgctggcgat ccatatttaa
    32581 tgaatgagga gacggatgct cgaattgaaa taaatcaagt aattgcagaa tttgagcgga
    32641 ttgcggacca agcaaaagaa ccagatattc gtcaatactt gttagaaatg aaagcagagt
    32701 tggaagtaaa tggtattaca gatgagtaac ctaaaccaac acaactgact gttaggcgct
    32761 gaccgcggag gattcgcgaa gcagaaatcc tccgtggtgc tgtgcctgtt catggacggc
    32821 gttagccgac catgatgtct cacccccgta tctaacaggg gggtacaaaa acacaaaaaa
    32881 taagtaagca accattaaga atgcttgttg tatcaaagat agtaagtaat ttgtataggt
    32941 gttaactcat ttgaaaaagt taacaccttg aatttggagg tgagaacttg gcacaacgga
    33001 atttagatta tcggctatta aaagaccgac gcaacgaata cggcgtttca caaaataaat
    33061 tggctacggc ttgtggactt agtcggccgt acttaaatca gatagaaaat ggtggcgtaa
    33121 ccgcatctat aaaaaccatg aggaaaattt ttgaccaact ggaaagcttt aatcccgatt
    33181 tgcctttgac gttactgttt gactatgtaa ggattcgctt tcccacaacg gatgcacgaa
    33241 aaattattca agaaattctc catttgaaat ttgagtatat gctccatgaa gattatgcct
    33301 tttactccta tcaagaacag tatgtcatgg gtgatattgt ggtgatgttg tctcatgaag
    33361 aagataaagg tgtgctttta gaattaaagg ggcgaggttg ccgacaattt gaaacttttt
    33421 tactcgctca aaagcgtagc tggtacgact ttttcgagga ttgtctaaag gccggtggcg
    33481 tgatgaaacg cttggacttg gcaatcaatg atcgagtggg gctattggat atacctgagc
    33541 taacgaaaaa atgtcagaag gaagaatgta tctccttgtt tcgtaccttt aaaagttatc
    33601 gttccgggga acttttaaag gctgatgaaa aggacggcat gggcaatacc ttatatatcg
    33661 gcagtctcaa gagtgaggtt tatttctgtt tgtatgaaaa agactatgaa caatatatca
    33721 agttagggat tccattagac caaactgaga cgaaaaatcg ttttgagatt cgactgaaaa
    33781 atgaccgtgc ttttcatgcg attcaagatt tgttaaaagg tcgtagcatt gagagcacca
    33841 cgttttccat tatcaatcgt tacttgcgat ttgcggataa agtagaaggt aaaaggcgaa
    33901 ccaactggcc cttgaatgaa caatgggggc gttttattgg acggaatcgg aaagagattc
    33961 aattaacttc cgagccaaaa ccgtatacca ttgaacgaac cttgaattgg ctaggccgac
    34021 aagtcgcccc tacgtggaaa atggctaagg agttggatcg attgaatcgg acaacctata
    34081 tccaggatat ggtacagaat gcacgcttat cagaccgaca taaaaagatt ttggagcaac
    34141 aaagtatggc aattgaaaat ttgattatat gaaaagagga atcgtcatgg gaattgtaaa
    34201 agatattttt ttggtatttg gtggcagtat gcttggtgtt actgtgatgt gcttgatgaa
    34261 tgcaagtgca gcagcggatc gagcaatgga aaagaaagga aagtgacaag atgaatttcg
    34321 gacaaaattt
//
LOCUS       DAZULO010000019        34023 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000019 DAZULO010000000
VERSION     DAZULO010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.019"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(112..471)
                     /gene="rplT"
                     /locus_tag="ACG8OA_000933"
     CDS             complement(112..471)
                     /gene="rplT"
                     /locus_tag="ACG8OA_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFS4975765.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(524..724)
                     /gene="rpmI"
                     /locus_tag="ACG8OA_000934"
     CDS             complement(524..724)
                     /gene="rpmI"
                     /locus_tag="ACG8OA_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFS4975766.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(796..1320)
                     /gene="infC"
                     /locus_tag="ACG8OA_000935"
     CDS             complement(796..1320)
                     /gene="infC"
                     /locus_tag="ACG8OA_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFS4975767.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1364..1489)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1640..2272
                     /locus_tag="ACG8OA_000936"
     CDS             1640..2272
                     /locus_tag="ACG8OA_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS4975768.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2274..3179)
                     /locus_tag="ACG8OA_000937"
     CDS             complement(2274..3179)
                     /locus_tag="ACG8OA_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS4975769.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3264..3923)
                     /locus_tag="ACG8OA_000938"
     CDS             complement(3264..3923)
                     /locus_tag="ACG8OA_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS4975770.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4239..5183
                     /gene="mvk"
                     /locus_tag="ACG8OA_000939"
     CDS             4239..5183
                     /gene="mvk"
                     /locus_tag="ACG8OA_000939"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFS4975771.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5176..6153
                     /gene="mvaD"
                     /locus_tag="ACG8OA_000940"
     CDS             5176..6153
                     /gene="mvaD"
                     /locus_tag="ACG8OA_000940"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFS4975772.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6166..7251
                     /locus_tag="ACG8OA_000941"
     CDS             6166..7251
                     /locus_tag="ACG8OA_000941"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFS4975773.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7248..8312
                     /gene="fni"
                     /locus_tag="ACG8OA_000942"
     CDS             7248..8312
                     /gene="fni"
                     /locus_tag="ACG8OA_000942"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFS4975774.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8561..9364)
                     /locus_tag="ACG8OA_000943"
     CDS             complement(8561..9364)
                     /locus_tag="ACG8OA_000943"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFS4975775.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9384..10226)
                     /gene="kduI"
                     /locus_tag="ACG8OA_000944"
     CDS             complement(9384..10226)
                     /gene="kduI"
                     /locus_tag="ACG8OA_000944"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFS4975776.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10242..11249)
                     /locus_tag="ACG8OA_000945"
     CDS             complement(10242..11249)
                     /locus_tag="ACG8OA_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFS4975777.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11256..11900)
                     /locus_tag="ACG8OA_000946"
     CDS             complement(11256..11900)
                     /locus_tag="ACG8OA_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFS4975778.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12000..12758)
                     /locus_tag="ACG8OA_000947"
     CDS             complement(12000..12758)
                     /locus_tag="ACG8OA_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFS4975779.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12885..14036)
                     /gene="fucO"
                     /locus_tag="ACG8OA_000948"
     CDS             complement(12885..14036)
                     /gene="fucO"
                     /locus_tag="ACG8OA_000948"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFS4975780.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14118..14429)
                     /gene="rhaM"
                     /locus_tag="ACG8OA_000949"
     CDS             complement(14118..14429)
                     /gene="rhaM"
                     /locus_tag="ACG8OA_000949"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFS4975781.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14431..15276)
                     /gene="rhaD"
                     /locus_tag="ACG8OA_000950"
     CDS             complement(14431..15276)
                     /gene="rhaD"
                     /locus_tag="ACG8OA_000950"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFS4975782.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15290..16546)
                     /gene="rhaA"
                     /locus_tag="ACG8OA_000951"
     CDS             complement(15290..16546)
                     /gene="rhaA"
                     /locus_tag="ACG8OA_000951"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFS4975783.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16543..18003)
                     /gene="rhaB"
                     /locus_tag="ACG8OA_000952"
     CDS             complement(16543..18003)
                     /gene="rhaB"
                     /locus_tag="ACG8OA_000952"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFS4975784.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18156..19025
                     /locus_tag="ACG8OA_000953"
     CDS             18156..19025
                     /locus_tag="ACG8OA_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS4975785.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19151..20050)
                     /locus_tag="ACG8OA_000954"
     CDS             complement(19151..20050)
                     /locus_tag="ACG8OA_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4975786.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20215..21039)
                     /locus_tag="ACG8OA_000955"
     CDS             complement(20215..21039)
                     /locus_tag="ACG8OA_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS4975787.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21050..22345)
                     /locus_tag="ACG8OA_000956"
     CDS             complement(21050..22345)
                     /locus_tag="ACG8OA_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFS4975788.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22361..22840)
                     /locus_tag="ACG8OA_000957"
     CDS             complement(22361..22840)
                     /locus_tag="ACG8OA_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFS4975789.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22846..23874)
                     /locus_tag="ACG8OA_000958"
     CDS             complement(22846..23874)
                     /locus_tag="ACG8OA_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFS4975790.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23891..24997)
                     /locus_tag="ACG8OA_000959"
     CDS             complement(23891..24997)
                     /locus_tag="ACG8OA_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4975791.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25226..27823)
                     /gene="adhE"
                     /locus_tag="ACG8OA_000960"
                     /gene_synonym="adhC"
     CDS             complement(25226..27823)
                     /gene="adhE"
                     /locus_tag="ACG8OA_000960"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFS4975792.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28128..28508)
                     /gene="yajC"
                     /locus_tag="ACG8OA_000961"
     CDS             complement(28128..28508)
                     /gene="yajC"
                     /locus_tag="ACG8OA_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFS4975793.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28605..29750)
                     /gene="tgt"
                     /locus_tag="ACG8OA_000962"
     CDS             complement(28605..29750)
                     /gene="tgt"
                     /locus_tag="ACG8OA_000962"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFS4975794.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29968..30666
                     /locus_tag="ACG8OA_000963"
     CDS             29968..30666
                     /locus_tag="ACG8OA_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS4975795.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30834..33473)
                     /locus_tag="ACG8OA_000964"
     CDS             complement(30834..33473)
                     /locus_tag="ACG8OA_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4975796.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
BASE COUNT         9597 a         7455 c         6152 g        10819 t
ORIGIN      
        1 ttttagactt atacgttatt gtcctgtttc atttgatgga ggattgtaaa taaaaaaaga
       61 ggaaagaaca taacttttcg ttatcttctt tcctctttat aattgtttag cttatttagc
      121 caaagcttct tttgcttggt cagccaatgc agtaaatgct gctgcatcgt taacagccaa
      181 gtcagctaac attttacggt tgatgtcgat ttcagcaact ttcaaaccgt gcattaattt
      241 tgagtagctc aagccattca tacgagctgc tgcgttgata cgagcaatcc acaatttacg
      301 gaagtcgcgt ttcttttgac gacgatcgcg atatgcgtag ttgtatgaat tcattacttg
      361 ttcttttgct gttttaaata aagtgtgttt tgaaccgtag taaccttttg ctaattttag
      421 cactctttta cgacgtttac gagttactgt tccaccttta acacgtgcca tgtataattc
      481 ctcctaataa aaataaatct tcagattctg ttatttgtac ttcttatttc atttttgcta
      541 gttgttggcg aatacgtttg aagtcgccac tagaaaccat gcttgcttta cgcaattgac
      601 ggcgttgttt ttttgttttg ccgtggaaac ggtgacttgt aaacgcgcgg aagcgtttca
      661 atccgccttt accagtacgt ttgaaacgtt ttgctgatcc gcggtgtgtt ttttgttttg
      721 gcatgactat atttcctcct caaaatcttt cgttatccga ctatcggctg tccaacagcc
      781 agttagtcat tcaaattact tactgtcgtt tttcggtgcc agcgtcaaga acatgctgcg
      841 tccgtccatt ttcgcttttt gttccactgt tgcaatatcc gcagtttctt cagctaagcg
      901 attcaagact ttttgaccaa tttctttatg ggtaatggca cggcctttga aacggataga
      961 agctttcact ttatcgccct tttccaagaa cttacgtgca ttacgaagtt ttgtattgaa
     1021 gtcattcaag tcgatcgtag gacttaagcg aacttctttt acattgatca ctttttgttt
     1081 tttacgcgct tcgcggtctt tcttttgttg ttcgaaacgg tatttaccgt agtccatgat
     1141 tcgcgcaact ggtggtttcg caccaggggc aactaacaca agatccaagt ttgcttgttc
     1201 tgcaatattc aatgcttctg cttttgtctt aactcctaat tgctcaccat ctgaaccgat
     1261 caatcgtaac tctcgtgcac gaatgccgtc gttaaccatc atatcctttg ctatggtcat
     1321 tcacctccaa atattatgag agaaagaaaa gcttaccgaa taaacggcaa taaaaaaacg
     1381 agttctctta cagaacccgc gcgtacttca cctaatagaa ccagtgaaaa ctatctagcc
     1441 cagtgacatt atcaactaag gcgagaagcg ggtgcttctg cttttatttc tcaaccatca
     1501 tagtatagca aaaactaatc tttttgtcaa tgtttttcat acagtttgtc ttccttttct
     1561 gaaaatgatt agattagcag actttctagc agtgattccc gcttctttgc tatactaaag
     1621 aaaaacgagg tgaacccaca tgaaaaaaat cgaacctatt ccaccttctc ttcctcttct
     1681 tacaaaggga gagttttatt tagaagatga ggagatcttc tctggtcttc tgatcaacgg
     1741 gggtgaccaa agctatctcg atgccaaaaa tatggtatta agagagagtc atatccaaaa
     1801 acttacgatg caaaaaacag ttttacgtca ttttgaatgc agtaatgtca tttttgaaaa
     1861 atgtgatttt tccaatctgg agtggattgg tggcagtttc caccaagtcg tgtttcatca
     1921 atgtaaatta acaggtacga attttgccga aagttatctt cgagactgta ctttcactga
     1981 ttgtattgct tcgatggctt cctttagtag tacaaacttg aaagctgtct ccttcgatca
     2041 ttgtcaatta gaagacagcg aattttatga ggtcacttgg aaaaaccttt ttttgtctga
     2101 caatcagttg accggttcga actggtttcg aacttctctg aagggattag actttacgac
     2161 taatttcttc acagcaatcg ctttatctca agattctttg gcgggactaa tcgtcaacca
     2221 agagcaagca cttgtgatcg cccaagccct tggtttgcag atcaaagact agatcaacga
     2281 atccgccgcc acagtgcaat aagcatccaa atagataaga ccaaactgat gatcaaggcg
     2341 attccccatc cgaaaacgga atgggtaaag ggtaactgga gattcattcc gaagaatcct
     2401 gtcacaatag ttggaacggt caagatcagc gaccagaccg tcaaaaattt catcgtgtca
     2461 ttcaagttgt tattcagcaa attattataa gtgccagaga gctggtctaa aatctgcgaa
     2521 gctaagttcg tcatttcaac tgcttgtcgt gcttcgatca gcgcatcgtc caactcttct
     2581 ttttcctttt ctgacaactt gcgataaata gccaatgcct tgatttgttc taaaagtact
     2641 acattttgtt ttgttcctgt aactaagtaa accaaaccga tctctaaatc tgaaagttgc
     2701 agcagattct tgttcgttgt tttttctctt aatttcagat taagccgctg acgttcacta
     2761 ttcacttcct cgattaaagg aaaaaaggaa tcagaaatca aaaacaatgt acgaaacaat
     2821 aaactgtata cactcatgtc tggcattcgt tccaaaagcg atcgaattag tcgaatcacg
     2881 tactcggtat tcggcgtggt aaatgtaaac aaaccgtctt cttttaaaat aaaagcgata
     2941 ggactagttt cataatgatt atcgactttt tgctggtgcg gaacatttga tacaagcagt
     3001 aacgcttcat caaaagaatc gtactctacg cgcgcccgtt catttttatc tagagaataa
     3061 gtcagcatct cgtcactaat gccatattct tcttttaagt ttttaacagc tagcacatcg
     3121 ttttgctgta cattgatcca aaaattacgg tcttgattaa aatagtgttt ttcccgcata
     3181 ctctatcctc ctgacatttg tttccagtat aaagaaaaaa gtcgctcaaa taaaggttta
     3241 ccctttattt gagcaactgt aaatcaaatg gctactttgg tttggcgaat ctctgctaat
     3301 ttttcttcga tcattttcaa gacttcttct tttgcttgag gatcttccac aaaattatag
     3361 cggtctccat cgatttggat ttttggactt tctttgtatt cttcatacca ttgatcgtaa
     3421 cgtctgttca attctttgta ataatcgtat agacttggat caaaggacaa ttgttcatac
     3481 gaacgaccac gtttttcgat acgctccaac atcgtatcaa aagatacacg aatatgaact
     3541 aataaatccg gatgtttttt atgggcagca tagggaagtt cttccatcat gttttccagc
     3601 agttcatcat acacacgaac ttctgtttct gttgctcttc ctagatctgc atttagatgg
     3661 aaaagcagtg aatcttcata aatcgaacgg tccaatacat tattgtcatc tttcattgct
     3721 tgtttgatgc ttgcaaaacg tttattcaaa aaataaattt gcagcaaaaa agcatactgt
     3781 tcagggtttg cataaaataa gggcaagact tcattatcat caatcgattc ataaaatgcc
     3841 tgactatcaa agtgttcagc catcatttct gttaaacttg attttccggc accaattgta
     3901 cctgctaaaa cgatcacgct catttacctc cagctcactt catcactaaa tattgtgttt
     3961 aactccaacc tttttaaata tacccccaat atctagtagt gtcaattgta tttttttcta
     4021 gtcaaagaag aaaaaaagtc aaaaaattct taagaaaaaa gtgttttttc tttagaaaac
     4081 gtattcttag ttttacttct gagatggttt tagtaaaata aaggaggaat gttttttgag
     4141 acccttagtg gacaaaaaaa ttcgtgaagc agcgcaactc gttttgggcg ctttattttt
     4201 tgcttaaaaa tcacttttac gtgagaagga gttagattat ggcaaactat ggccaaggag
     4261 aatcaagcgg aaagatcata ttgatgggcg agcacgcggt tgtttatgga gaaccagcga
     4321 ttgcctttcc tttctatgca acaaaagtca ccgcattcct tgaagagctg gatgcaatgg
     4381 acgatcaact ggtttcttcc tactattcag gaaatttagc cgaagctcct catgcattaa
     4441 aaaatatcaa aaaattattc attcacttaa aaaaacagca tgacatccaa aaaaacttgc
     4501 aactgaccat tgaaagcacg attcctgctg aacgtggaat gggatcaagc gctgcagtcg
     4561 ccacagcagt cactcgtgct ttttatgatt acttagcatt tcctttgtct cgtgaaatac
     4621 tattagaaaa tgtccagctt tcggaaaaaa tcgcccacgg taatcctagt ggaatcgatg
     4681 cagccgctac tagcagcttg cagccgattt attttacaaa agggcatcct ttcgactact
     4741 tttctttgaa catcgatgct tttttgattg tcgctgatac aggaatcaaa ggacaaacaa
     4801 gagaagccgt caaagatgtc gctcacctct ttgaaaccca gcctcatgaa actggacaaa
     4861 tgatccaaaa attaggatac ttgacgaagc aagcaaaaca agcaatcatt gaaaattcac
     4921 cagaaacgtt ggcacagaca atggatgaat cacaatcact tctggaaaag ctgacaatca
     4981 gcaatgattt tcttaatcta ttgatccaaa cagcaaaaga taccggggca ttgggtgcca
     5041 aattaactgg cggtggacgc ggtggctgta tgattgcatt agcacaaaca aaaacaaaag
     5101 cccaagaaat cagtcaagca cttgaagatg cgggtgctgc tgaaacttgg atacaaggat
     5161 taggagtaca tacctatgtt taaaggcaaa gcacgcgcat ataccaacat tgctctaatc
     5221 aaatattggg gtaagaaaaa tgaagaactc atccttccaa tgaacaacag cctttcgtta
     5281 acattggatg cattttatac agagacagaa gtcatctttt ctgatagcta tatggtagat
     5341 gaattttatc tagatggcac cttgcaagac gaaaaagcaa caaaaaaagt cagtcagttt
     5401 cttgaccttt ttcgtaaaga agccggcctc tcactaaaag cttcagtaat cagtcagaat
     5461 ttcgttccta ccgcagctgg attagcctcc tctgccagcg ggctagctgc tttagcaggc
     5521 gcttgcaata ctgctcttaa gcttggatta gatgatctct ctctttcaag atttgctcga
     5581 cgcgggtctg gttcagcttg ccgaagtatt ttcggtggtt tcgtcgaatg ggaaaaaggc
     5641 catgacgact taagttctta cgctaagcca gtcccttccg attctttcga agacgattta
     5701 gcaatggttt tcgttttgat caacgaccag aaaaaagaag tgtccagcag aaatgggatg
     5761 cgtcggacag tcgaaacatc cagtttttat caaggctggt tagattccgt tgaaggggat
     5821 ctatatcaat tgaaacaagc aatcaaaaca aaagatttcc aacttctcgg agaaacgatg
     5881 gaaagaaacg gactaaaaat gcatggaacg actttagctg cccagcctcc attcacttac
     5941 tggtctcctg acagcttaaa agcgatggat gcggtgcgac aattgagaaa gcaaggcatt
     6001 ccatgctact ttacgatgga tgctggccct aatgtcaaag ttttagtgga aaacagccat
     6061 ttatctgagg tacaagaaac atttaccaaa ttatttagca aagaacaagt aattactgca
     6121 catgctggtc caggaattgc gattattgaa taaagggaga tttttatgat tgaagtatct
     6181 gcaccaggca aactctatat tgccggagaa tatgcagttg ttgaaacagg ccatccagca
     6241 gttatcgctg cagtcgatca attcgtgaca gtcactgtag aatctgcacg aaaagtcgga
     6301 agtatccaat ctgcacaata tagtgggatg cctgtacgtt ggacaagacg caacggagaa
     6361 ttagttttag atattcggga aaatcctttt cattatatcc ttgctgctat tcgcttgact
     6421 gaaaagtatg cacaagaaaa aaacattctt ttatcctttt atgatctgaa agtaacaagt
     6481 gagttagaca gttcaaatgg ccggaaatat ggattgggtt ctagcggtgc cgtgaccgtt
     6541 gcaacggtca aagccttgaa tgttttttac gcgttgaatt tatctcagtt ggagattttc
     6601 aagattgcag cactagccaa tttagcagtt caagataatg gttcctgcgg cgacatcgct
     6661 gctagctgtt atggtggctg gatcgctttc tcaaccttcg accatccttg gctccaagaa
     6721 caagaaactc agcattctat cagtgagtta cttgccctgg attggccagg tctatccatt
     6781 gagccattga ttgctcctga agatttacgt ttattgattg gttggacggg tagccctgcc
     6841 tctacttctg atttggtcga tcaagttcac cgttcgagag aagataaaat ggtggcttat
     6901 cagcttttct taaaaaatag tacagaatgt gtcaacgaaa tgatcaaagg gtttaaagaa
     6961 aataatgtaa cgttgatcca acagatgatt cgaaaaaacc gacaattact gcatgattta
     7021 tctgcaatca ctggggtcgt catcgaaacg cctgctttga acaaattgtg taatttagct
     7081 gaacagtatg aaggagccgc aaaatcttct ggtgcaggtg ggggcgattg cggaatcgta
     7141 attgttgacc agaaatctgg cattcttcct ttaatgagtg catgggaaaa agcagaaatc
     7201 actccactgc cgttacatgt ctatagcgat caaagaaagg aaaaccgatg aatcgaaaag
     7261 acgaacatgt ttcactagct aaagcctttc atgataaaca aaaaaacgaa tttgacttcg
     7321 tacggatcat ccataaccct ttgccgcaaa tagcagtttc tgatgtcgat ctaagtacac
     7381 aggcagttgg gtttacacta agcagcccat tttatatcaa tgcgatgact ggcggaagcg
     7441 aaaaaacaaa aaaaatcaac caagatcttg caatcgtcgc aagagaagca gatttgatga
     7501 ttgccactgg ttcagtcagc gctgccttga aagtcccttc tttagccgat acttatacga
     7561 ttatgcgaca agaatatcct cacgggaaaa tcattgccaa catcggtgca gggacttctg
     7621 tcgaaagagc caaagaagct atccgattgt ttcacgcaga tgccttacag attcatttga
     7681 atgcacctca agagttggtt atgcctgagg gagaccgtga ttttaccaat tggaaagtac
     7741 ttattcaaga aactcaaaca gccattgacg tccccctcat cgtaaaagaa gtcggttttg
     7801 gcatgacaag agaaacgctc aatgatctag ctgctttagg agttcataca gttgatatca
     7861 gcggtcgaag cggaacaagc ttcacccaga tcgaaaacgc ccgtcgctcc aagcgggaac
     7921 tgagttattt agctgactgg gggcaatcga cagtctcttc attgcttgaa gcaaatgaag
     7981 cagacacttc tatggaaatc ctagcctccg gaggtatccg caatgcctat gacatattta
     8041 aagccctttg tctcggcgca aatgccgtgg gtacttcagg aaccgtactg acgcatctga
     8101 tgaatcacgg cgtagaagaa actattatac tgatgaagca atggcaagaa gagcttcgtc
     8161 tgctttatac tatggtcggc gcaacgaata ccgcggccct acaccaacaa tctctgatct
     8221 tttccggacc agtgaaggat tggtgcgaag caagagggat cgacttagtg aagtatggaa
     8281 gacgcacaga aaagagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca
     8341 cgtacaaacg gcggaagtaa aagcaactcc ttcggaaata agctgaaatt cacaaaaatt
     8401 tgaaaagcaa ttttcgtgaa ttccttctta tttcttggag ttaaatgctt ctgtcccagc
     8461 ctcttttttc gaataaatga tgtttaatca gctgtccttc ggtattcgtt caatccgctg
     8521 atttcacaac ctctccacta aacagtgtac taaagctctt ttaaggttgt tttccgatat
     8581 aggcaagtat gcctccgtcc acatacaaga catggccgtt aacgaaatca gatgcgctag
     8641 aagccaagaa gacagatggt ccagccaaat cgattggctc gccccagcgt gctgctggcg
     8701 tacgtccaac gataaattga tcgaatggat gacgttcacc gttttcttgt gtttcgcgta
     8761 atggagcagt ttgcggtgta gcaatataac ctggtccaat tccattacat tggatattgt
     8821 attgaccgta ttcagaagcg atgtttttcg tcagcatttt taatccgcct tttgccgcag
     8881 cataagcact gaccgtttcg cgtcctaatt cactcatcat ggagcagata ttgatgattt
     8941 taccgccgcc tttttcgatc atatccggaa taactgcttt tgaaacgatg aatggggcat
     9001 ttaaatccac gtcgattact tggcggaagt ctgccgcatc catttcgatc atcggtgtcc
     9061 gtttgatgat ccctgcattg ttgactagaa tatcaactga acctacatct tctttgatct
     9121 gttgaaccat cgcttgaacc gcttcttcat cggtcacatc gcagacataa ccttttgcat
     9181 cgatgcctgc ttctttataa tgcgctatac cttcatcaac tgattcttga ttcaagttat
     9241 taaatacgat ttttgctcct gctgtagcta aagagcgtgc aatctcaaat ccaataccat
     9301 agacagcacc ggtgactaat gctacttttc catctaaacg aaacatatcc atactaaatt
     9361 ccatcatttt ctctccctta tgcttatttt aattgatcca tttgcaccat atccatgtct
     9421 gtataggtga tattttctcc gcacattgcc cagatgaatg agtaattgct tgtacctaca
     9481 cctgaatgga tcgaccagct tggcgaaata atggcttgtt cattgtctac aactaaatgt
     9541 tttgtttcat ctggtttgcc catcatgtgg aacacacgtg tatcttcatt ggcataatca
     9601 aagtagacat aagcttccat acgacgttca tgtgtatgac aaggcatcgt attccaagcg
     9661 cttcctggtt ctaaaatcgt ataacccatt tgtaattgac agctttcgca tacatttgga
     9721 tggatgtatt ggtagatttt acgttcattc agtgtcaggc cttcaccagt ctccattggt
     9781 tttacttggt cgatactgat ttttacattt ggatatttat gatgagctgg aacagaacta
     9841 atatagaatt tagctgggtt ttcagcatct tgagaagaaa atacaacatg tcgtgtttct
     9901 tttccgatat agtatccgtc ttgttttttc atttcttctt tttgcccatc gatctcgatg
     9961 aatcctggtc caccgatatt gatcacacct agttcacgac gctctaagaa gtaatcaaca
    10021 cctagttctt tgtctaatgt gatttctaat tctttttcag taggtgtcac tccaccaaag
    10081 atcattcgat cgttgtgcgt ataagtcaaa ctgattttcc ctggttcaaa taccttttct
    10141 actaaaaatt cttttctcaa ttgttccgtt gaataatgac ggatatcttc tggactgtgt
    10201 gtataacgtg tttccatttc ttgcattttc ttctactcct tctatcgaat gatttttcca
    10261 gagccgcatg ctaaaaattg tgcgacttct tcttgactaa attggttgca gtccccatgg
    10321 atcgtatgct tcaaggcaga agctgccgta gcaaaatcaa tgatttcttg cggagacata
    10381 ttggataaaa tcccatggag cacgccacct gcaaaagcat ctccgccacc tactcgatcc
    10441 acgattgggg tgattctatg gacttgggat tccacatatt catggttcat ccacaatgtc
    10501 cccttcaatt cattttcact tgctgaatga acggtacgct tggtcgaata aaacacctga
    10561 atattcggga acttttcctg cattttttga taataataaa ttagaggttg ttcttctgct
    10621 tctggcgctt tctcgatacc taacaaataa agagcatcca tttttccagc tgagcagata
    10681 tctactaaag gtaaaagctg atggattact tcgctggctt cttgctgtgt ccataatttt
    10741 ccacgatagt tgatatcgaa gctgatcagg cagccagctt tttttgcagc ttctatcacc
    10801 tgtttcgtca ttttttgcca ttttttagac aaagctgggg tgattcctga aacatgaaat
    10861 atatctattc cttggaataa ctcctctagc gaccattcca aatgatccat ttcagcaaaa
    10921 cttgatccgg cacgatcata gatcactgag gcggcgcgtt cgccactacc agtttccata
    10981 taataagtac ctagtcttga tccaccagat aatacctgtt tcgtatctac tccataacat
    11041 tgcaagtgct ttttgactgc ttttcccaat gcgttatcag gtacttttgt agcaaaaatc
    11101 gcttcatgtc cgtaattagc taaagaaatt gctacgtttg cttcccctcc gccataatgg
    11161 gcagcgaatc gttcacttga atcgatacgg gttccctgct ctgtggacaa tcgcaacatg
    11221 atttctccga gtgtcactac ttttcccata agcagctacc tcttgatttc tttcattttt
    11281 gctgcatact gttgcgcgac ttccgttact ttgtcaaaat ctcctgtagc tgcgggtgct
    11341 aaaaggttcc cacctactcc gactgttacg acaccagcat cgaaccactc tttcatattg
    11401 tccagactca caccgccagt tggcatgata ttgacttgag gcatcggcgc cttgaaggca
    11461 gaaatgatac ttggtccata agcgcttcca ggaaatagtt tgacgatatc taccccgctt
    11521 ttcagcgctg ttttgatctc ggtaatagtc atacatcctg gtaagtaagg aatttgatac
    11581 agattgcaca tctctgccgt ttcttgatca aaggaagggc taacaatata ctctgcgcca
    11641 gctaagatag ccaagcgagc tgtgacagcg tctaatactg tgcctgcgcc aatcactatt
    11701 tctggttggt ctttgtagaa tgacaaaagt tctttgatta cttgatctgc ttgagggact
    11761 gtaaaagtca actcaatacc agttagacca cctttgatga ttgcgtggca agcattcaaa
    11821 gcttcttctt tgctttttcc gcgtaccacc gcaatcactc cggcgttttt caatcgagat
    11881 aaaatatcaa tttttttcat cttctccacc tatttcctat ttagaaattt tattttataa
    11941 cttggaaatc tatacccata ttatacggca gaaaagaaaa attgcaaacg cttttttcat
    12001 tatttttgaa ttctttgctc gatttcgctt tttgtttgct tgagggcact gattatttgt
    12061 tcttttgtct catctgttaa acgatatttc ggcatactta cactaaatgc accaatgatt
    12121 tgttcgtttt tcacgattga ggctcctata caaaaaatat cacgttcgat ttcttcatcg
    12181 tcaaatgcga tgtgggtttc ttttaccgtt ttgatttcct ttttcaaccg taatgcattg
    12241 gtgatcgtat attctgtgta agggatcaac ggatgctttt caagataatc agtgtactgc
    12301 tcttcactga attctgctaa tacggctttg cccatcgcag aattgtataa tggtcgggtg
    12361 attccgattt ttgaagacat gcggatcgtt tgatgttttg gatctagttt gttcacgtac
    12421 aagatctcat tgttgtccaa tacccctaag tggatagtct catcgatctt ttgctgcagc
    12481 tgttctaaaa acggtaaagt caactctacc aaatcgattt gttcaatgtt tttattcgcg
    12541 tagcgaataa gctttgcacc taaacgatag tttttctctg aatttttatt tacgtagccg
    12601 atcatcaaca aagtatctag tattttcaag actgtagaag aggtcattcc cacacccttt
    12661 gcgatcgtct gcaaagagac atcttggttt tccgctaaat agtctagtat tttagaggct
    12721 ttgatcaata cagttccata aggcttttta ttttccatat aatattctcc tatttcttta
    12781 taagaaaaat tatagcggaa ataccatcaa tttaaaagag atatccagat ataaactacc
    12841 atttctcttt acaggcaatt taatgttgaa gatccgcttc gtttttacat caaactttga
    12901 tataaagcaa tgatttcttc tactgtcgtt tctcttggat ttcctggtgt gcatacatct
    12961 cgcaaagcat cttctgcaat tgctggaata tctgcttctt ttacacctaa ttcagaaatg
    13021 gtttctggga ttccaaccgc tttgctcaac cgatcgatct caccacaagc cgcttctctt
    13081 acatcttcta aagacatttc agctgctcct ttaatcccta gaacaagagc gatctcacgg
    13141 tacttttctc cagtatattc tttgttatat ttcatgacag ttggcaaaag tgctgcacac
    13201 gctacgccat gaggaatgtt gtaccatgca cttaacgggt gagccatccc gtgtaccagt
    13261 cctaaaccga catttgagaa gcccattccc gcaatatact gtcctaatgc cattttttct
    13321 cgcccatttt gatcgccatc tacagaagct tccaaagaat ggccgatgat ttcgattgct
    13381 tttaaatgca gcatatcagt catttcccaa gcgccttttg tgataaaccc ttcaatagca
    13441 tgcgtcatag catccatacc tgttgatgca gccaattttt tcggcattcc catcatcata
    13501 tcgctgtcga tgaacgcgac gattgggata tcatgaggat caacacaaac aaattttcgg
    13561 tgatttactt catctgtgat gacgtagttg atcgttactt ctgctgctgt tccagaagtc
    13621 gttggtatag ctaatatagg taaacatgga tttttcgtat cagcaacacc ttctaaacta
    13681 atgacgtctg aaaactctgg attcgtcaaa ataatcccaa tggcttttgc cgtatcgatt
    13741 ggcgaacctc caccgattgc gatcaaacag tctgcctctg cctctttaac aaaagccact
    13801 ccatctttta cattttggat cgttggatta ggtacgatcc cgtcgtataa agcataagcg
    13861 attgctgctt tatctagaag ttctgttact tttgttgcta taccgtgctc gatcaatcct
    13921 ttgtcagtaa tgacagctgc tttgccgaat cctcgctgct gaaattcagg gacgatcgac
    13981 tcaatcgctc cttttccaaa atacgatgtt tcatttaaaa tcattcgatt actcattttc
    14041 tattcctcct aatttgtatt atttttcaca ataaaaacga aagaacccca agcaaccggt
    14101 tgcccagttg cttgttgcta cagttcaaag actggcatca attcccaact gactggagag
    14161 gcatctggat ttgtctccat gatatcttcc atgtacttcc accatttttg attgatagat
    14221 gtatttgcca ttttttccca taacttttca tcttcgatca ctaagtaacc aaataacaaa
    14281 cttgtactcg gatcaagata gatcgaatag tcgatgaccc cgtgttcacg aagtgctgta
    14341 cgcatttctg gccaaagttc atcatgtctt tttttgtatt cttcttgttt ttctgggtaa
    14401 actttcatgc aaaaagcttt tttcaccatg tcagacacct gctttcgtat gaacttcctc
    14461 caaataacct gcttttggag tgacaccaaa cgcatctgct aaatgccaca ggttttcgtc
    14521 cgtgattgtt tgcaagattg gtccttgcgc tttgacgtac gtatagactt ctgctgcctt
    14581 ttctgctgtc tcgatcaaac caaagacttc atccatgtct tttcctgacc cgtagattcc
    14641 atgttgtggc cataaaacca atcgagtttc tttcattttt tcagctgttg cttccccaat
    14701 ctcatttgtt cccggaacca tccacggaat gatggaaatt ccttctggaa aaacaactaa
    14761 gcattccgta cacatttgcc atagcgtacg actaaaggaa cgttcgtcta attcatgcgt
    14821 aaaactcatc gctaatagat gactagcatg gcaatgcata acgatccgat tatctgggtc
    14881 tacttctaaa cgggcgatat gactcatgaa atgacttggc aactcgcttg taggaaggca
    14941 ttcgttttcc agcccccaca aaagttccag tgttttgcca tcttcagcaa ctcgaacgat
    15001 tccaagattc tcggcaggtt cgctcgctac atttttaaaa tatcttcctg aaccagttac
    15061 aataaaatag catcctgcta aacggctggc atcaaaaatc atcgagatgc ggcggatcac
    15121 tttttcagga tccaagaacg gtgtgatctc ttcttctttt agtaaataag aaatattccc
    15181 gccatttctt tcatcccatc ccaagcgata aagattcgac gtcgtttcga tcatttcttt
    15241 cacataaggc gcttgtaaaa tatctatttt tttcaatata gccacactcc tattttctta
    15301 attccaacac gtctttttga tattcctgga cttcttccag ccaatccaaa ccaactggaa
    15361 cttgattttt gtaacagaaa taattccata catcggcata cgggaaatct ttcaattctt
    15421 ccgtataagc cattcttgaa gtaaagtcaa aagccagctc catttctttc aatcgctcaa
    15481 ctggttccag catagctttc agcagtgctt tttgtgtatt tctcgtaccg atgacccaag
    15541 cagcgatacg gttgatcgtt gcatcaaaga aatccagacc gatatttgtt ttttccaaca
    15601 attcgttacg gactaattct ttagcgattt cctgcaattc atcatccatc aaaaccacat
    15661 ggtctgaatc ccaacgcact ggacgggaaa catgcaacat gattcctttg ctgaataaag
    15721 ctaatgaaga taatttattg gaaatcactt ctgttggatg aaaatgtcca gcatctagac
    15781 aaacaagttt gtcacgagtc aacccgtaac ccatataaaa ttcatgggaa ccgactgtat
    15841 aagcttcagc gcctaaacca aatagtttgc tttctactgc atcttgtaca taccgttcat
    15901 ctaactcttc tgtaaaaatt tcatcgagtg attccattaa acgttcccgt ggggtaaaac
    15961 gatcaatcgg attatctttc atcccatcag gtacccaaaa gttattgata cttgtttgcc
    16021 cagtttcttt cccaaagtac tccgcgattt tgcggcttct tttcccgtgt tcgatccagt
    16081 attcacgaac ttctttatct ggatgagcca atgtaaagcc gtctttcatc attggatgcg
    16141 aaaagaaagt cggattgaag tcaagaccga tttcttcttt ttttgcccac tctacccatt
    16201 tttcaaaatg tttcggctca atttcattta gatcgacaga ttcttctgta tccaaataga
    16261 tagcatgaag gttcaatttg tgtttgcccg gtatcaacga ataagccttc tccagatctt
    16321 gtctaagctc ttcaggtgtt cgtgcggctc ctggaaaatt ccctgtcgcc atgattccac
    16381 cggtcaattc gccatccggt gtcaagaaac ctttgacatc atctccttgc cagcaatgca
    16441 tagaaatttt gatgtcctgt atcttttcta gtacagcatc tgtatctaca ccgatagttg
    16501 catatttttc ttttgcttgt tcgtaatttc tttcaatcgt gttcatacga ttactccttt
    16561 gattttattc acgtaccatt ccatcgtgtt ttgcttattt tcatctgggt aaaaagtttc
    16621 acatggaaac gactgtttga ttacatttct tgcttgttct atattcgaaa actgacctgt
    16681 actgagcatt tgcatggcaa cattcccgat tgctgtggct tcactaggtc ctgcttctac
    16741 aatgattccg gcataatctg ctgtcaattg attcagtaga cgattattcg atccgccccc
    16801 tacgatgtgc agcttttcca atttcgttcc agttaagtgt tctactttga tcagctcttg
    16861 tgcgtaacaa agagcaagat tatcatacac acaacgagcg atagctcccg atgagacagg
    16921 gactggctgc tctgtttcaa aacagtattg ctgaatctct tctatcatat ttgggggatt
    16981 caaaaatcga gggtcattga tttccatcac ttgctgcagc ggtttttctt ttgcagcaag
    17041 ctctgccaat tccgcataac tgtactcgta ctcttgcatt cgggcaactt cctggatcaa
    17101 ccacatcccc atgatgtttt tcaaaaaacg caccgtgtta tgggcacccc attcattcgt
    17161 ataattttca gaaaaagcat tgcgagaaac attggcgatc tttgtttcaa tccctaaaag
    17221 cgaccatgtc ccactgctga tataacccca acgttctcct tgacctggcg ttcctaatac
    17281 agctgacgcc gtatcatgac ttgctactgt gataaaggtc acatctggca aatcatattt
    17341 tgaaaattgt tctttttgca gatctcctag tatcgttccc ggctctgtca atggtggaaa
    17401 taattccttc gagacaccga ttttttcaag aagttctatt tcccattctt ttgtccctgc
    17461 attcagcagc tgcatagtag atgcattagt tttctccgtc accatgttac cagtaaagac
    17521 ataccctaaa taatcaggta tcagtaacaa tttatgtgct tttgccaaat tttctttttc
    17581 ttcagcaaac aattggaaca aggtattgaa aggctggatc tgaatccctg tctgctcata
    17641 caacttatct agagaataga tttcagaaaa ctgttctact gctccattgg tacgcttgtc
    17701 ccgataagca atcggatcat ttatccgatc cccttgttca tcaatcagac agtaatcgac
    17761 tgcccatgta tcgatccccg caaaaaggcg atcaatccct gtctgcttta atttttcaaa
    17821 tccaatcaaa atctctttga taagataatc gatcttccag cgatcgtgac cgtctttgtt
    17881 ccgaaagccg tttttgaacc ggtgtacttc ttccaagatc aaagaacctt ttgcatccct
    17941 cgtacttttc attagtcgtc cgcttgatgc accgatatcg atggcaacaa atgttggatt
    18001 catttctcca cctcttttac tttattgttg attagcactt tattaataaa gcgttatcat
    18061 ttattgacaa tgctatcata atacagaatc ttatattttg cttcacacta ttttggtata
    18121 taattgcatt atcttgacaa gttgggagtg tttacatgga ttactgggaa aaagccaacc
    18181 actcatggtc ggaagactca aaaagagtga tcttgacgcc tacacaaaga agcaggaatt
    18241 tattttttta tatccaagaa atcggttact tcaaagcacc aatcccttac tatactgaac
    18301 gagcacattt accttcttac ttgataaagt ttactttgtc tggcaaaggt tcgcttacat
    18361 ataatgataa tcagcaacag ctaaaagctg gagacttatt ttttattgac tgccaagaat
    18421 atcagcgtta tcaaacgatc agcgaagaac catgggaaat ggattggatc catttgaatg
    18481 gtcagaacgt ccaatcattt tatcaggagt tcacaaaaga cggccataac accttccatt
    18541 caacaggaga tccatcagaa aacaagatcc accatttgat cacagaattg atccacatgc
    18601 aacaaggcac gaatgcccgt accgatttcc agtcctctgt agctatccac gagttgctga
    18661 atgaactgat tttgcaaaag tatcagctag atttcgaaga agaggatatt cccgcctacg
    18721 tttacaaact gaagcaatac ttagacgaac atttcaaaga aacgattaca cttgatcaat
    18781 tagaacaaca attcttattg aataaatatc agctgagcaa agatttttcc aaatttatcg
    18841 gttctccacc aatcgattat ttgatcagca aaaaactttc ttacgctaaa gaccttcttc
    18901 ggtatacaga atttacggtt cgaatgattt ccgaagaaat agggattgag aattttgctt
    18961 actttagccg cctgttcaaa gcaaaaacag gactatcgcc gagagacttt cgtaaattca
    19021 gctgattcat ataatatgta cgtcattttt tcaaaatatt ttgaaatata tagataaaaa
    19081 taaaataacc gcagtttctg cttctatgta aacaacagca gctactgcgg ttatttttta
    19141 gtgatcattc tcattgttca tcttcaaaat gttcataatg aaccaactgt ttctgtgtat
    19201 cacgtttcaa aaattcttta ggcgttatgc cgaactctct cttgaatgct ttcagaaaat
    19261 gcggataaga agaaaaacca cttttactat atacagcact agagctgcgg tattctcgca
    19321 acaagtgctt ggcatagatc agctttttct tcattacata ctgatgaaac gtaaatccag
    19381 tgtacttttt aaattctcgt gtgatatggt atttgctgac aaaaaactgc ttttccattt
    19441 cctctaaaga aagcggatga gataaatttt ttgagatata ttgtaacatt tgttccacac
    19501 gctcattagg aactacagca ttctgttctt gcgcactctc ttctttttgt acaacttgat
    19561 tgagaaaaat caagtattct accagagctt gttcatattt tatgtcttca ccgtaaattt
    19621 ttttatcagg aggatggtcg ataaatgata atttttgctt caatacttcc tgttctattt
    19681 gcaatacacg actcctcttc tctcccattg gttgaaaaca agccgataga ttagaccatg
    19741 gcgtggaacg actttctaga aaagaagggg tgacaaagat atagacacgc tcgaatacat
    19801 ctgaagattg gcggacgata cgatgcaaat cattggaatg gatgagcagc acacttcctg
    19861 ctttgatcgt aaattgtcgt ccgttcaaat aaaaagtcgc ttctcccttt aatgtagcat
    19921 ggatctcata gaaatcatga tgatgataga tcgttttatt ttggctgacg acatctttga
    19981 catgatagat ttcatactga tcagaaacca tcgtattgac ttgttcaccg ctgattttct
    20041 tttggagcat gtgccgcacc tccttttgtt cacttatgca caattttatt ataccacgtt
    20101 ttttgtaatc aaaaagagct gcttttatat actcttataa aaaagaagag acgtggcatc
    20161 ttctagcccc gccccctcca ttctaaatat tagttaacca acgtcttctt tacattattc
    20221 aagaagactt cttccataac gaatcccatc atcttttgtt tcttcaagaa ccacatacag
    20281 gtacggacga ttcttcatga tgatttcttt gatttttgtg tactcgatca caccgtctcc
    20341 caaatttacc ggcacgattt tttcctgttc gacacgaaaa tctttcaaat gaaccgcaca
    20401 tatcttttca ccaaaacgtt caaaagcttc ttctaccaat tggacttgtt cttgataatc
    20461 actacttgtg atcaaattgg tcggatctaa aatgattcct agatagtccg aatctactgc
    20521 ttggatcagc tgctctaccc ttgctaaaga atacaccgga tgattcaaac ctggttctat
    20581 ccccaccatc atttggtatt tttctccagc ttttaccaag cgacggataa cggacacagc
    20641 ttcagcaaat gcttcatctg tgaaattttc ttccgtatat tggatttccg gcaaaacaca
    20701 tccagtttcc gaagcaacca tcgacgcacc aaaggaagaa gcatagcgaa gatactgctc
    20761 gaactttgtc agcaactctt gtcgtatcgt taaatctgga tgaatcatat tgatatagca
    20821 acttaaaatt cctacggtca cttcttgctt ttctaaaact cgtttgacat aatttcccat
    20881 cccagaattg atctcatttg cgccagaagg tatttcagga aacgaaagac ctaaggcgag
    20941 ctgcagtgta tgaatccctt gttcttttgt tttttctgca agatcttcta tcgtttggac
    21001 ttcggaaaga tcatgtcctc taattgtaat gttcaacata actattctcc taactcagca
    21061 tattgggtag ccacatgacg atttgcggga agtaagtaat caaaaacaag acgactaaga
    21121 taaccccata aaaaggaaga agcggtttca gcattgcctc tgcttttacg cctcctacac
    21181 ttgcaccgac atatagtcct gtacctactg gcggcgtgat ggagcctaca cacagattca
    21241 tgattgaaaa caacccgtaa tgtacaggat caacccctac acttgtgacg attggtaaaa
    21301 atattggtgt aaaaatcaag atagcaggcg ccacatccat aaacataccc accagtaata
    21361 acacaagatt gactaataac agaataataa atttattatc cgttacgccc ataatcaacg
    21421 aactaattgc ctgaggaata ccggtaaacg ccatggcgaa agacatcatg gaagaagtag
    21481 ccaataagaa catgatcgtc cctgacattg ctactgattg aaacaacata ttaggtatat
    21541 cttttagttt taccgtttta tagtaaaaca tagcaatcaa cagggagtaa atgacagcta
    21601 tcgctgaagc ttctactgct gtaaatatcc ccgtcaagat accaccgata atgatgacaa
    21661 tcaatagaag gctaggaatc gcctctttta ttatttttcc agcatgagcc atctgtcctt
    21721 tttcgacaac agggtattta ttctttttag caacgatgaa tgctactagc atgattccta
    21781 gcgcccacaa agcgcctacg agataacctc ccataaacaa agcagagatc gaagcaccac
    21841 cagcaacaag tgaatacatg ataaaagcag tacttggagg aatgaccata cctgtcggag
    21901 ctgacgcgat attgaccgct gtcgcaaact tccggtcata tccttcttcc acttgctgag
    21961 ggatcaacac tccgccaatc gcggtagaag cggcaatggc cgagctagag atggaaccaa
    22021 ataaagcatt ccctaacaca ttcgtatgag ccaaagcccc cggcacgcgc ccgacgaaca
    22081 acatcgcaaa atccactagt ttcttagcta tccctccatt attcatgata atgcccgaga
    22141 gaacgaaaaa cggaatagcg atcagcgaaa aactattgat acttcccacc attttttgag
    22201 ctgaactaaa agcagaaaca tcaaacggta taactaacaa taaagtcatc atcgatgaga
    22261 atgcaatact tatagcaatc ggcatcccta atacaagcaa cacaacaaat acaaaaaata
    22321 acactaatcc ggcttctatt gccattttat tcctccattt ttatattttt tcttgaattg
    22381 ctggcttgtt gcttaatgta tcgattagat tgatcaggct atagataatg atgagtaccc
    22441 ccgatacagg tactgacaga taaacatatc ccataggaat acttaaagaa ggcgagatct
    22501 gccccattgt caatgtgaca gcattccatc cgccatacac catgatcatc gcagcaaata
    22561 ccagaaataa actttgaaca acaacttcta aaattttact ttttttgcca gttaattttt
    22621 cacttaaaac cgttacggct aaatgtgctt tttgtcccac aacatacgcg gaagacaaca
    22681 tggcaaacca gacaagaccg aatcgaacaa gctcttccgt gaccgtattt ggatttccta
    22741 aaaccgttct ggaaaatact tgccataaaa caacagcgac catcccaaca agcaaaacag
    22801 ctcccaggat ctctaatacc ttgtttaaaa tcattttcat cacagctacg ctccttgctc
    22861 ttggatcttt tgataaaggt ctttgtaggt aggatcgttt ttgaatatat catgaagtgg
    22921 ggcaactgcc tctcgaaacg gtgtattatc tacatcattg aattggactc catgttcttc
    22981 ttgtgctaaa gctttttctt cttcaattgc ttttgcaaaa gcttcaattt caaaaaccgt
    23041 tgattctttc gctgcttctt caatggcttt ttcctgctca tcagttagct gttctttgat
    23101 ggcatcattc atgatgatga tatctggcac acgtgtatgc tcatcatagg aatagtattt
    23161 agccacacca ccatgtccag ctgtatacaa gacaaattca ttgttttctg atccgttgat
    23221 caaattggat tgaagagacg tgtacacttc atcacttccc ataggtacgg gcgaaccgcc
    23281 taaaagctct accattttga tagcggtttc gctttgcatc acacggatct tctttccctt
    23341 caaatcttcc ggcgtatgga tcggaccgtc taccatataa aagctgcgct gaccagaatc
    23401 ataataggtc aatccggtaa atcctaaatt tttggtcgat tggtagattg gctccattat
    23461 ttcttcatta tccatcacct taaagaaatg ttcttcatca ttgaatagat aaggaactga
    23521 aaaaatcgaa taatctttag agaaactttc caacgctgag gcacttactt ttgtgaaatc
    23581 tactgctcct gactgtgtca attcgatcag ttcacgttct ccacccagtt gtccatctgg
    23641 gaaatactgg atctgtacgg aaccatctgt tttttctttt actaaacgcc caaattcatt
    23701 cattgcatca attactggct ggctgttgct tgcataagca aagcgcaagg taactatctc
    23761 atttacatct gcatctgcga cagttgccac actagaatta tttccgcagc cgcttagacc
    23821 tagcagacct aggctaagcg aacttgctgc aaacagcagt gatgtttttt tcatatgggt
    23881 ttcctttctt ttaacaataa gagacgagca attctgtcaa acaaagcacc gttaacgact
    23941 gtccataagg cattgctgtg attccgatat tccgataaaa atctaaactg tcccccattc
    24001 ctgtcccgat tgaaacgttt tgaacttctc ctttttcatc gatttgttca agcaatcctt
    24061 ggatagcacg ataagcgact tctttatatt cttgtggtaa ataacgttta tggaccgctt
    24121 tcaaaatacc gtaagcaaat cctgcagttg cagaagattc tacgtaagaa gaaggatcat
    24181 ccaacaacgt atgccataaa ccgctttcag actgataagt ttttaatgca cgcacttgtg
    24241 cttcaagtgt ttcgatcaat aatttgcgca atgcatcttc tttcgataat tctaagattt
    24301 caatgatctc tggaatagca atcgtcaacc agcagtttcc acgagcccaa aatgcttcgg
    24361 catagttatg atttccttca aacgtccagc cgtgatacca caatcctgtt cgtttatcca
    24421 tcaagtattt gatatggatc ataaactggt aacgagcttc ttctaaatat tctgggcgat
    24481 tcaacagttt cccgattttt gccaaaggaa gcactgtcat catcaatgta tcgtcccaca
    24541 actgattctt attttccggt ccatatgtgg catgctgtaa cccgtcttca tttgtccgag
    24601 gcatgtcatt catgacccat tctgcccatt gttctaaata aggcaaatag cgtgaatctt
    24661 tcgtgtcttc atacaagtaa gccatagtca atagcggtgc cattgtattg acgtttttgg
    24721 gcggagcgcc ttcttccata cgatcctcaa accattcatt gaccacttga taagcgcgag
    24781 gattttttgt catctggtaa tttttataaa taccatatag accgacacct tgtggccaat
    24841 tccacactgt ccagcttttg tcatctactt tcagtccatc aaagtccaac aaaaattcac
    24901 cattatcgtc tttgatcgtt gtcaggtttt caacgagcag gtcaattttt tctttcacta
    24961 attctttagg tacaaaaatc tcttctttaa taatcatctt tacgccacct ttttaaacgc
    25021 tttcatttta tatccttaat atagcagtaa taccgtccac tataaatatc gctttttgaa
    25081 caaaaactgt ctaaaattga cctaaatgtt aaataacaaa agtaactttc agttacttgg
    25141 aaattaaaaa aagaacttgg agaaacaacg ttttccaagt tctttttcat ctattttctt
    25201 atcttttttc tacgctttct tttgcttatc cgttgtaagc atcaatgatg atctgtttca
    25261 attcagagat caatggttct ttcggattag ctgtcgtaca ttggtcttcg taagctaatt
    25321 cagccatacg gtctacggtt gtatctagtg tttcttgtgt gactccttga gcttttagac
    25381 tcatgttgat gccgacagat tttcctaatt cataaacagc cgttgctaaa gcttcgacta
    25441 attcctctgt tgtttcgcct ttcagaccta agaattttgc gatatctgcg taatctgtat
    25501 ctgcacggaa atagtcatat ttagggaaca tcgcatgttt ttgtggatct tttgcattat
    25561 agcggataat atgcggtaaa agaatcgcat ttgtccgtcc atgagggatc ccgtattccc
    25621 cgccgatttt atgtgcgact gagtgacaaa tacctaggaa tgcgttagca aacgccatac
    25681 cagccattgt agaagcgttg tgcatttttt cacgtgattc catatcaggt gtttttactg
    25741 atttttctaa atagtcaaag actaacttga ttgcttgtag gctcaatcca cgtgtataat
    25801 ctgaagccat gactgaaaca taggattcga ttgcatgtgt taacacatcc atcccggtat
    25861 ctgctgtgac agatgctgga actgacatga cgaattgagg atcgacgatt gcgacatctg
    25921 gcgtcaatgc ataatctgct aacggatatt tcacatgtgt ttcactgtca gtgatgaccg
    25981 caaatggcgt tacttcagaa ccagtccctg atgttgtcgg gatacatacg aattgtgttt
    26041 tttctggttt gtcgatctta tatgtccgtt tacggatatc caagaatttt tgttttgcac
    26101 caaagaaaga tgtgtctggg tgttcataga acatccacat tcccttagct gcgtccattg
    26161 cagaaccgcc tccaagagcg attactgtat ctggcttgaa gtctgccatc agtttcgttc
    26221 ctgcgtatac ggtatttgtc gatgggttcg gttcaacatc tgagaagact tcgatcttca
    26281 catcattttt acgtcgtgcc aatacttcac gaactgtatc gcagtaaccg aattggacca
    26341 taccagggtc acaaacgatc atgacacgtt caacgttttc cattttctct aagtacaata
    26401 gagagttctt ttcaaagaag atttttggcg gtaatttgaa ccattgcatg ttatttctcc
    26461 gtttcgctac agttttgaca ttgattaagt ttacagcaga gacgttacga gaaacagagt
    26521 tcttaccata agaaccgcat cccaaagtca atgacggaat catttcgtta tagatatcac
    26581 cgatgccgcc ttctgcagat ggtgtattca ctaaaatacg acaagctttc attcgaagac
    26641 cgaatttgac atgcaactct tcgttttctg aatggataac tgctgtatgt cctaatcctc
    26701 caagattcaa cataccttca cacaaatcaa acgcatggtc cgtgttgtta gctttgatca
    26761 tggcaagaac tggagaaagt ttttctcttg atagcggata atctgggcct acgccttcca
    26821 attcagcaat cagcattttc gttccttttg gtacactgat tcctgccaat tttgcgattt
    26881 ccatcgcaga atgtccaacg attgatggat tgaccgcata ttttccttcg ttcatcactg
    26941 catcttctaa tttttgcaat tcatctggtt ttacgatata aacttggtgt gcttggaatt
    27001 ctgcttttac tgctgcatag atttctttat ccacgatcac agcttgttca gaagcacaaa
    27061 tcattccatt atcaaatgtt ttcgaaacga tcaaatcgtt cactgcacgt ttaatttttg
    27121 ctgttttttc aatataagct ggtacgttac ctggacctac acctaaagct ggttttcctg
    27181 tagagtaagc agacttgacc attccagctc cacctgtcgc taagacgatc gcgatacctg
    27241 gatggttcat tagcatagaa gtcgcttcga tcgaaggatg ttcgatccat tggatacaat
    27301 tttcaggagc tccggcagcg atagcagcat cacgtaccac tcgtgctgct tctgcagaag
    27361 atttttgggc acttggatgg aaagcaaaaa cgattggatt tcttgttttc aacgcgatca
    27421 ttgatttgaa aatcgttgtg gaagtcggat tcgttgttgg agttactccg caaaccacac
    27481 cgactggttc agcaatttca atcaaccctt tttggacatc ttcattgatc acaccaactg
    27541 ttttatcatg tttgatatta tgccaaatat attcggacgc atacatattt ttgattgctt
    27601 tatcttcgta aacaccgcgg cctgtttcct ctacagccat cttcgctaat ggcatatgtt
    27661 gatccaaagc tgccattgcc atttcatgca cgatatggtc tactttttct tgatcaaagc
    27721 tttccatttc tttcaatgct gcatttgctt tttctgccaa atcatcaatc attgcttgta
    27781 cgtcaattgt cttcgttttt tctttttcta ttactttagc catcattatc ctcctaatgg
    27841 gtcatggtta tttgttaaat aattcacatg tatatcatac aatatttatt tttctatgta
    27901 aacacttttt tgtgattttt ttcacagtta tttttaaata taaaattgct ttatttacag
    27961 gtgtttgtat agaaaaatat ataaagttat ttattattat ttatatgaca cacccgatta
    28021 ttcaatattg agcaatatat cccatgctct tatctctttc ttttttgaat aaaaaatgag
    28081 gtaggacatc tttctgccct acctcgtctt taaataagct ttgatactta tttttcttcc
    28141 ttctcttctg tctcttccag cgtttctgtt gtttcagttt ttacttcaac tgtctcagct
    28201 ggttcttctg ttgtttcaac agtagtcgtt gcatcatttg ttacgacagt ccctggtttg
    28261 accgttttga ttgctgcacg atcgtactca aggaagatcc cttcgcagtc taaaacaact
    28321 gtccgtttgt cattgtcgat ttctgaaaca actccatgta atccgccgat cgttacgact
    28381 gaatcacctg gtttcatgct atctaacaag ttttgacgtt cttgctgttg tttcttttgc
    28441 gaacgtgaca taaaaaacca cattgctaat agcgcgataa acatcacaag cattggtaat
    28501 gttcccattt ttttcacctc actaattctg tatctacaaa aaacacttta gcagaaaaat
    28561 gctgataaca ctagtttttt acttaatttt taaaagattt tcctttagaa gctttttgcg
    28621 ttttctttat tgaacccgta ttcttcaaaa aaggcttctc taaattcaag caaattatca
    28681 tccatgattg cttgacgtac ttgtttcatt agattcaaca agaagtataa attgtggtaa
    28741 gaagtcaaac gaatcccaaa tgtttcatca catttgatca aatggcggat ataggcacgt
    28801 gtatagtttt tacatgtata acaatcacat ttctcatcaa ttggacggaa atcatgggca
    28861 tatttcgcat ttttcactac tagacggcct tgagaagtca tacatgtgcc atttcgagcg
    28921 attcgagtag gcaagacaca gtcaaacatg tctactccac ggataacacc atcgatcaat
    28981 gaatcagcag caccgacacc catcaagtaa cgtggtttgt tttcggggat caacggtgta
    29041 gtgaattcta atacacgatt catttctgat ttaggttccc cgacagataa cccgccgatt
    29101 gagtaaccag ggaaatccat cccgactaga tctttcgcgc tttgacgacg aagatcttca
    29161 aaccctgctc cttggatgat tccaaataat ccttgacggt ctggattagc atgagctttc
    29221 aatcctcgtt ctgcccatcg agacgtacgt tctactgatt ttttcacgta gtcgtaactt
    29281 tcatcgaatg gtggacattc gtcaaaactc atcatgatat cagagcccaa tttattttgg
    29341 atgttgattg ctttttctgg tgataaaaac atacgcgaac cgttcaagtg atttttgaaa
    29401 tgcacgcctt cttcttcgat attccgcata tcacttaatg aaaaaacttg aaagccgcct
    29461 gaatctgtta aaatcggttg atcccagttc ataaatttat gcagtccgcc agcttcttct
    29521 actaagtctt caccagggcg tagccataaa tgatatgtat tacttaaaat aacgccagca
    29581 cccatttctt tcaactcttc tggggacatt gtttttactg tagctaatgt tcctactggc
    29641 atgaacattg gtgtggggaa agttccgtgt ggcgtgatca attcccctaa acgcgcgcca
    29701 gtatgttttt ctttcttgat taaacgatag cgaatggcag gttgcgtcat tcatttccct
    29761 actttctata taaaatatct tgacaactcc caccatcata atcgtttcaa gtgctttttg
    29821 caagttccct cggcttcctt ctactttatt taatattctt ttttttatcc tttttttatg
    29881 aataacaaaa aaaagaaaaa cattatgaca gaaatatttt ttgatacatg gtatattggt
    29941 gttattaaag aaaaggaggg aaaaatcatg caatcttctg cagaattaaa acgccaagca
    30001 aaagagagtc taaaaggacg ttggggacaa gctgttttac taaatctaat ccctacattg
    30061 attggtattg ctactattct tcttattatg attcctatta tcttgtttgc caccaccata
    30121 tatgatggat cagcgagcgt ctcagaagcg acaacttcag gaggcggtag ttcaggaggc
    30181 ggtggcggaa ttatttccag catcattagc gctttgttca tgagcggtat ttcttggact
    30241 tatcttgata ttttacgtgg acaaaaagat cagatcgaac cattcaaaga tgcatttcgt
    30301 ggattcaaag gtttgtttat cggcggagtg atcagcttag ctgtgctgat ctcgatcttt
    30361 acaacattat ggggtttgct attagttatt cctggaatca ttaaaagcta ttcttattct
    30421 caaagctatt ttatttacta cgatatcgtc caagaaacag gacaaacacc taaagtactg
    30481 gatacgatca ctgcaagtcg aaaattgatg gatggttata aaggacaact tttctggtta
    30541 gatctttcat ttatcggctg gcatatctta gcattagcaa ctgctggtat tggttatcta
    30601 tggctcagcc catacatttc tgcaacaaaa gcagcatttt atgaacaatt gccaaaagat
    30661 atttaaaaga gagtgtgact tgtgaccgag ggggtggcag gagaacaccg tttatcgaga
    30721 ggcagtaaaa gaaacattca gcccattagc aaaaaggagg tgtgacaaca ttcatgtcac
    30781 acctcctttt tgtatagttc cagtaaaaag aaaatcaact tagaaacagt taatcattcc
    30841 tcttcttctg cttccggtaa tcgatggaac ttgatcagtt cattcaccgc gattggtacg
    30901 atagataaaa tccctaccaa gaaccagtga ttcaaactga tgtgtactag gccgaatagt
    30961 tcttgcgtaa atggcagcaa agcaacagcc gttgtaatag ctaatgccag taacgccatt
    31021 tctaccaaag ctttattggt cgctaaattg actttgaaga tcgattggct gctgcgtaca
    31081 ttgaacacat gcagaatcga tgaataagct aagatcaaga atgcaacagt ttgaccgaca
    31141 tgctgacttg cgtctaccca tgggctgata gtatcgacat aactacctaa ataataacca
    31201 aacaaggtta cgatggtgaa gacccctgca tttatggcaa ttttttgcaa taaacctcga
    31261 gcaaagatcc cttcattttt cggaatgggt ggctgatcca tgatgccgtg ttccgctggc
    31321 tctcttccta aagcaaatcc tggaagtcca tcagcaacta cattgacaaa cagtagttgc
    31381 acagctgtaa atggtgctcc ccatcctaac agcatcgcaa tcaaaacaat aaagatctcc
    31441 gaaatattac agcttagcaa aaagttgata gctttacgaa tattctgata aaccgaacgt
    31501 ccttgactga cagcgtctac gatcgtcgca aagttgtcat ccgtcaaaat catatctgcg
    31561 gcactttgcg ccacatctgt tcctgtgatc cccatggcac acccaacgtt acttgctttc
    31621 aacgctggtg catcattcac accatcccca gtcatcgcta cgactgcacc cgtacgttgc
    31681 caagatttga cgatccggat cttgtcttct ggcgttaccc gagcatagac agagagagct
    31741 ttgacacgcg catctaattc ttcgtctgat aattgatgca gttctgatcc tgaaagtgct
    31801 tctgacggat cattcaagat acctaattct ttcgctatgg cactggcagt caccacatga
    31861 tctccagtga tcatgactgt tttgatgcca gcctttttcg ctcgcttgat tgctccttta
    31921 ctttctggac gtggtggatc gatcattccg atcagaccaa tcaaacgtaa gtctttttcc
    31981 agtgcttcag aagaaatatc ttgcggttct tcgtcataag tagcataacc aacagcaatc
    32041 actctcagtg cccgtttacc aaagcgatca ttaacgatcg ccgcttgttc tacatcgcct
    32101 gaactaaagc gcggagcaag aacatcgaat gctcctttgg tgattgagat gtatttttcg
    32161 cccatttggt gaactgttgt catcattttt cgttcagaat caaaaggaag ctcgtttact
    32221 cgtggatatt tttcttctaa ttctgccttt gtatgataat tctcttcaac agcccttacg
    32281 atggctactt ctgttggatt acctttgata acaaggtcgt catcttcttg ctcgaccgtc
    32341 acatctgtac atagtgcagc cattttcaat atttccatcg cgctgttcgt cattgaatct
    32401 tcgatattcg ttacttcatc ttctttgctc catacccggc ggacgcgcat ttcattttgt
    32461 gtcagcgttc ctgttttgtc tgagcagata acattagcgg ttcctagcgt ttcaactgct
    32521 ggcaactgtc ggatgatcgc atgtttcctc gccatcttct gcacaccgta tgcaagtgtc
    32581 aacgtaacaa taacagtcaa ggtttccggt acagctgcaa cagccaagga aatcgaagtc
    32641 atgaacatct caaggagtgg ttctccttgc agttcaccaa tcacaaatac taaagccgca
    32701 gccgctaaag cgatcatact gattcgtttg cctagctgat tcaatcggac ctgtaaaggt
    32761 gttttttgca tagtattttc gttcaaaaga tccgcgatct tacccatttc tgtagccatg
    32821 ccaatggcag tgacgatcgc tttcccacga cctgcagcaa ccgtacatcc tttaaatacc
    32881 atattaagtt ggtcacctaa tgactcatct tcttcagcaa taaatgctgc atctttttca
    32941 actgcttcac tttctccagt caacgccgat tcttctacac gtaattgtga tgcttcgatc
    33001 aaacgggcat cggcagaaat agcatcaccg gactctaaaa caagaatatc acccactacc
    33061 agttgctctg actcaacagt ggtcactttg ccgtctcgta tgacagttgc cgtttgttta
    33121 ttcatatctt gcaaggattc cagtgctttt tctgcgtttc cttcttggac aatcgccaat
    33181 accgagttga tcacaacgat agcgatgatc agcagtcctt caaataaatc cccatgttct
    33241 gtagcaaatg cggtataaaa ggaaatcacc gctgctacaa gaaggatgat cgttgtaaaa
    33301 tcggacaagc ttcgaaagaa ttttttgatc atcgattctt tcggcgcctc atcgaattta
    33361 ttcaatccct gcttttttcg ccgttcatcc acttcttgtg aagacaaccc agtatctgga
    33421 tctgcttctg ttaccttctt tacttcttct attgtctgct tgtattcttc catcctattc
    33481 ctcctttaaa atcttcgtac aaaaaaaggt acttatttta ctataacatc ctttttttat
    33541 ttacttcaaa taaagggctt atcgattatt tgttaagaaa gcgtaaaatt taaagtgctt
    33601 atatttgcaa acgctaccaa aggatgctat tatatagatg taaggaagga ggataccatc
    33661 aaatagacag aagaatttct cgatagaaca gtgtaagcaa acaaagttgt tagaagtaca
    33721 ggtttcaccc agacatgcgt gtagagtaca aacctataaa tagtgaatgc gtatggatgt
    33781 ttggatcacg aagtatccga aacaagaagt aatgcgatac gtaacaattg aataacgtaa
    33841 acgtctgaaa attgaatagg aacaagagat gtttcaaatg ttgaagatgt aacgaaaaca
    33901 aaatttttgt ttaacatgaa gaaattcaac accgtgcttt gcatgtatga tggaaagtta
    33961 caatcatttc tgtttatttg aatccttccg aggtacaaga aagccgagct tatcgagagc
    34021 tcg
//
LOCUS       DAZULO010000020        33539 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000020 DAZULO010000000
VERSION     DAZULO010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.020"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..654)
                     /locus_tag="ACG8OA_000965"
                     /pseudo
     CDS             complement(1..654)
                     /locus_tag="ACG8OA_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            802..1943
                     /locus_tag="ACG8OA_000966"
                     /pseudo
     CDS             802..1943
                     /locus_tag="ACG8OA_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(2069..3148)
                     /locus_tag="ACG8OA_000967"
     CDS             complement(2069..3148)
                     /locus_tag="ACG8OA_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4975797.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(3251..3691)
                     /locus_tag="ACG8OA_000968"
     CDS             complement(3251..3691)
                     /locus_tag="ACG8OA_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4975798.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3748..4158)
                     /locus_tag="ACG8OA_000969"
     CDS             complement(3748..4158)
                     /locus_tag="ACG8OA_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS4975799.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(4338..4772)
                     /locus_tag="ACG8OA_000970"
     CDS             complement(4338..4772)
                     /locus_tag="ACG8OA_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4975800.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4872..5720)
                     /locus_tag="ACG8OA_000971"
     CDS             complement(4872..5720)
                     /locus_tag="ACG8OA_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4975801.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            6109..7875
                     /locus_tag="ACG8OA_000972"
     CDS             6109..7875
                     /locus_tag="ACG8OA_000972"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFS4975802.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7977..9236
                     /locus_tag="ACG8OA_000973"
     CDS             7977..9236
                     /locus_tag="ACG8OA_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4975803.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            9383..10684
                     /locus_tag="ACG8OA_000974"
     CDS             9383..10684
                     /locus_tag="ACG8OA_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4975804.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            10687..11538
                     /locus_tag="ACG8OA_000975"
     CDS             10687..11538
                     /locus_tag="ACG8OA_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFS4975805.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            11551..12366
                     /locus_tag="ACG8OA_000976"
     CDS             11551..12366
                     /locus_tag="ACG8OA_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFS4975806.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            12517..13683
                     /locus_tag="ACG8OA_000977"
     CDS             12517..13683
                     /locus_tag="ACG8OA_000977"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4975807.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            13913..14560
                     /locus_tag="ACG8OA_000978"
     CDS             13913..14560
                     /locus_tag="ACG8OA_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS4975808.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            14644..15588
                     /locus_tag="ACG8OA_000979"
     CDS             14644..15588
                     /locus_tag="ACG8OA_000979"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFS4975809.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            15723..17174
                     /locus_tag="ACG8OA_000980"
     CDS             15723..17174
                     /locus_tag="ACG8OA_000980"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFS4975810.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            17195..18031
                     /locus_tag="ACG8OA_000981"
     CDS             17195..18031
                     /locus_tag="ACG8OA_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4975811.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            18031..18690
                     /locus_tag="ACG8OA_000982"
     CDS             18031..18690
                     /locus_tag="ACG8OA_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4975812.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            18816..19505
                     /locus_tag="ACG8OA_000983"
     CDS             18816..19505
                     /locus_tag="ACG8OA_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975813.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            19511..20467
                     /locus_tag="ACG8OA_000984"
     CDS             19511..20467
                     /locus_tag="ACG8OA_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFS4975814.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            20511..21023
                     /locus_tag="ACG8OA_000985"
     CDS             20511..21023
                     /locus_tag="ACG8OA_000985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4975815.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(21279..21827)
                     /locus_tag="ACG8OA_000986"
     CDS             complement(21279..21827)
                     /locus_tag="ACG8OA_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS4975816.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(21916..23097)
                     /locus_tag="ACG8OA_000987"
     CDS             complement(21916..23097)
                     /locus_tag="ACG8OA_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFS4975817.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(23333..24841)
                     /locus_tag="ACG8OA_000988"
     CDS             complement(23333..24841)
                     /locus_tag="ACG8OA_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFS4975818.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            25000..25191
                     /locus_tag="ACG8OA_000989"
     CDS             25000..25191
                     /locus_tag="ACG8OA_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975819.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            25394..26311
                     /locus_tag="ACG8OA_000990"
     CDS             25394..26311
                     /locus_tag="ACG8OA_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFS4975820.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            26304..27377
                     /locus_tag="ACG8OA_000991"
     CDS             26304..27377
                     /locus_tag="ACG8OA_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975821.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            27525..28043
                     /locus_tag="ACG8OA_000992"
     CDS             27525..28043
                     /locus_tag="ACG8OA_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975822.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            28024..28416
                     /locus_tag="ACG8OA_000993"
     CDS             28024..28416
                     /locus_tag="ACG8OA_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFS4975823.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            28492..29427
                     /locus_tag="ACG8OA_000994"
     CDS             28492..29427
                     /locus_tag="ACG8OA_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS4975824.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            29560..30339
                     /locus_tag="ACG8OA_000995"
     CDS             29560..30339
                     /locus_tag="ACG8OA_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS4975825.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            30332..30649
                     /locus_tag="ACG8OA_000996"
     CDS             30332..30649
                     /locus_tag="ACG8OA_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS4975826.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            30893..32503
                     /locus_tag="ACG8OA_000997"
     CDS             30893..32503
                     /locus_tag="ACG8OA_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS4975827.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            32660..33508
                     /locus_tag="ACG8OA_000998"
     CDS             32660..33508
                     /locus_tag="ACG8OA_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4975828.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        11094 a         5986 c         6511 g         9948 t
ORIGIN      
        1 taatttatct atcataccgc tatttagtcg gtcaagtact tcttttttaa aaaaagtatt
       61 catcataaaa gccactactt gcggatcttc atcataaaat tctgtagcag tacttgaaaa
      121 gatgttccaa taaatatcta atgctgcata ttgaatttgt acctcttttc cagaaaaaga
      181 ctttctgcca ttattagaaa aggagataga caaaccgtac tatgctaaaa atgcattaat
      241 ctttttcatt ttcgtataca aataagaggc actatagttt gtttgctgac tgaaactgat
      301 aatcgaattt gtctgacagc tgaacaaaga agtcaagatc tgataactga ctgattcgtt
      361 gcagtatctt ccaaatagac gataatagca gtaagccgga tcaattcctt caaaattcaa
      421 ctttaaaaaa ggtttttgct tatcaatatg cagttctgtt agattgagac gctcaaagtc
      481 ttgttctaat tctttgatgt atagtaaaaa atttgattca gacatatgga aatgatctgc
      541 taaatattgt gtagtaataa agaaacgatg ttcagatagg aaaagaaaaa tatctaattt
      601 tgtattgctt tttttcttca agataaacgt tttaatcgct tcttgagttt gcatgaaaga
      661 ggctcctttt tacttaaatt gttaaaactt gaaaatatta ctgttttttt cataaattta
      721 gacaaaaaaa cagtaaacat ataggtagaa tctctactat cttatttctt gcatacaaaa
      781 aaatcaagga gtgagagagt tatgagatat tcaaccatct taatatcaac atttgcagcc
      841 ttaagcttat cgcttactat aaatcagaca gtccacacag aggagacaga aacagccaaa
      901 tggacagaaa ccaagtgggc caacgtcaat cgaattacct ttcaggatga attttcatcc
      961 ttcaattcag gagtcgcaaa attaagaaaa ggacaaacag cgaccatcaa agcaaaaatt
     1021 gactattcgg gagacttagc acctatacta caatcagctg ttacatttga gaatgttgat
     1081 cccactttgt cgattggcta tgacgagaac actttgacta tccaacacaa gcaagcgata
     1141 tttaccttca ccgttacatt aaatcaggac ttgttaaaac cagcgctttt tactgtcaag
     1201 gtatcagatg gactaccgaa cgatcatcat ttaacgccgt atagtcagcg gcagacaatt
     1261 gagcaagatt cagccatcga ctcaggagga gatcttgttg aagaaccaac agataaacca
     1321 gaaaatgaaa ataagccaga agtaccgcct acagaaaatc cagacggaga acagaaaccg
     1381 gaaatagaac ctggagaaga acccgataca gaaactcaac cagagccaga caataaatca
     1441 aaacctgaaa taacccctgg agaaaaacca gatgtaactc cagaaccaga tacggattct
     1501 ggaaaccaaa cagtaccgga aacaaatcca gacacagata atgaaactga aaatccagaa
     1561 aaaccagagg tagatccaga agaaaaacca gatgtgactc cagaaccaga tacggacgca
     1621 agagaccaag gaataccaga aaaaataaac aaaaaaacaa ttcaagagga tggaaaaaaa
     1681 gagagtaaaa aatcaaatct agcaattcta aaaataaatg aagagcagct gaataaaaaa
     1741 tcacgaatat ttgatagcgc ccaatcagct gaaaccttaa aaagtagcaa ggatacaacc
     1801 tttgctgtcc tgaaacgaaa aataaacagc taccaaaatc cggagagtca caaaacaaag
     1861 ttatactgtg gagcggaatc attctattaa gtatagcaac aatgctttct gcaaaaagat
     1921 ttaaacaaaa cagatctctc taacatttga ataatgtctg tgaacgcatt tttcaaataa
     1981 aaagaaatat ccagaaaatg accccaaaat accagattga aatctgatat ttggggtcgt
     2041 tcatacttta tctttcttca tatttatttt aagggatatc tgtttctttt ggaagatggt
     2101 tcgggatctt tttcactttc aatacatcta aaaagaaggt aaagatcgga atgccgacaa
     2161 tcaagcccca aacgccaaag aaccgttcgc taacaagtaa gaccacaaat gtataaaaaa
     2221 tcggcagttc tgttttgctg gacatcaact tcggattcag cacataagac tcaattagat
     2281 ggacaatcaa aatcgtaatc aggatataaa caacatctcg gatgcctcct tgggaataag
     2341 ctatgaaaga tagaggaata caagaaatga taacacctgc tactgggatc agacttaaaa
     2401 tgaaaatcat gatcgctaaa ctcagcagct gatgaaagcc aaatacagcc aaggcgattg
     2461 tagtgagcaa cgtatttaaa agagcgacaa caaattgagc ttctaatacc aaaccaaatg
     2521 tattgacaaa cttatcggca aaatagtaaa tatcttgaaa gaaccaagaa aattcacttt
     2581 ttaaaaacaa tcttgagaat acaattgttt ttttcttttc aatcataaaa aagaaactta
     2641 agatgaatga catagcaaac gccactgcca tttttcctat atcctgtaaa tagccaagcg
     2701 caatcgaagc gccattttga agctgttcaa aaaaatcgtt tcgctcaata taagtatgga
     2761 tatattgtaa aacaggatct gcatcttttg gctgatgctc gtaaaaatta ataacggact
     2821 ggatcatgtc aaatgtttga tgaataagca tcgggatata ctttgtgaat gccagataga
     2881 taaggtaaac gatcagtgta taaatcatga tagctaaaac acctgtaggg attcggacaa
     2941 atcgctgaat gatttgaaca agatgaatca tcaaataagt aaagataaat gtgagcaaaa
     3001 tggttgtaat catacttcta actaagaaaa gaacaaaaac gacaaataat aaaacagtga
     3061 gacggcgtat tttttcattc gccaaaaaac gctgataaac ggtcaaatcg acaacctcca
     3121 aataacaaaa aataaacacg ttattcatta tatgatggaa tatgaaaata agaaagagca
     3181 aaaagaaaaa ccgctaaatg acgttatctt atgagttggt tttcctcata aaatcgttca
     3241 tctagcagtt tcagcccttt ttctggtctt gatgaaaatt atctagcatt tttcgggtca
     3301 gatgttgcag tgtatccaat tcttcctgtt caagtggaag ataagacttg atcttttgtg
     3361 gaataccttc tgcttgtttt tgcaacgcag caccttgagc agtcagcgta acatttacga
     3421 ttctttcatc acccgtttca cgctgacgat taatataatt tttttctgct aactttttta
     3481 gtaagggagt cagcgtacca gaatccagac acaaatcatt tcctagctct ttcactgaag
     3541 tctttttctt ttcccataaa actaacatca caagatactg ggtataggta agattcagcg
     3601 gcttgaggag gggagtgtag tgtctaatga tttctttaga ggcggcatat aaaggaaaac
     3661 atacttgatt aactaaatat agatcttcca tttttccacc tccgctgttt tactatagtg
     3721 tatcatagcg gatttacaca aaaaagtcta ttcactaact gctttcagag taacttctat
     3781 atttccagct gtagcttttg aatacggaca cactttatgc gctgcctcta ataattcttg
     3841 cgtttcttcc agagaaagac cttcgataga tccttcaatc tctacaccta agacaaagct
     3901 cgtttcactt tctgaataaa gggaaacttg tgcactgaca gtagaagccc cagagatatt
     3961 tttttgctgt ttgatcaatt ctaatgcact gttaaaacat gcactgaaac cagctgcaaa
     4021 caattgttca gggttggttg cattttcgac gcgtttacca ggagctgcaa tattcaattc
     4081 gaatgtacga tcgggattat gcacttctcc agagcgtccg cctgtgttga tcattttcgt
     4141 agaatacatt tttttcataa aattaccacc tttaattata ttttatacaa ttgaatttta
     4201 cacaatataa ttgtggaaag caaagaaatg gattggcata agataaataa gagaaagcac
     4261 atgaaacaga cgcgctttct cgaatagcaa acatatacaa aaaatatcag agacaaaaaa
     4321 taatctcatc ataaacatta catatatttc tcaaaaagtt gttttctttc ttcaaaaatt
     4381 acttcacgat ggacctgatg aagcatccat aaataaccaa aaggatctct gaacattgag
     4441 ttagtcacac caaagtccat catttctgtc atttcttgaa tctctacaca gccatttcgt
     4501 aatgctttat tatgtgtgtc ctcgatatca gagacgatga tattgaacca gatcgtattt
     4561 ggatgttcag cagtaggagc agtcaaacca agagacgtgt tttcatccag catatggaat
     4621 ctcgtaccat acaaggtaaa aatgacttca ttattgcctc tagggtaatc tgttgcctca
     4681 atcagttgta tttcatcaaa gattgtttga tacagttcta gtgctttcaa actatcattt
     4741 aatacaaaat cgatttccgt tcctttatgc ataaagatct cctctccttt tcagtcgaac
     4801 agttactaga ttcttttatt ataccagcta aagaaaacgc attcaaaaaa atagcggatt
     4861 tacttaccaa ctcattcgat tggatgataa gtaaatccgc gtcccattac ttctgctacg
     4921 ctgctgacaa tgacaaaggc acttggatcg atttcttcta tcaatggttt cattcgggaa
     4981 aattcatgat cagtcacaac gatcaataaa acctcttttg actttcgatt atacccgcct
     5041 tttgcctcta gcagggtaag gccacgtcca atctcagcac ttaagcgaag gcggatgtct
     5101 tccaaataat gttcactcat gaccataaca gatttctttc gattgatacc tgtttcgata
     5161 taagtcatga caattgaagt gattgcaata gataaaatag cataaagaaa ctcttctatt
     5221 ccaaaaataa acagattaag agaaacaacg aatgcgtctg taaaaaataa gccaatcgaa
     5281 gtattcaagt ggaaatattt tttaaaaatg aggggaggga tcgtcgtacc gccgctagaa
     5341 gagttgtttt tatataaaat cgcaactcct aaagcaaaaa tcgcacttcc aaaaatgaca
     5401 gacaacaacc gatctgaagt aatcattgtt tctggaacta ttgcaatgat taaaggaaaa
     5461 accaagcttc cgaaaagtac tttaaaaaag ggttttttac ctaaaaatag aagagctagt
     5521 accaacataa cgatattcaa cactaaaata actatagctc ggtcaaagcc taaagcagct
     5581 tccagtaata ttcctagacc gctgacacct ccagcagcaa tatgatgagg ccctaaaaac
     5641 atattgatag cgactcctaa tatgaagagg gcaaaaataa tcttaatgta tgtccatata
     5701 aattgtctcg tttctttcat ttattcactt ccttaattca ttaataatac tcttatctta
     5761 ttataactta cttttcacct taagttaact ttaattaata aaaaaattag gctagtctaa
     5821 attattatgt ttcattcttc tagaatatac ttctacgcca aaaactgtta aaaaactttt
     5881 actttagaaa attggagaaa ttgatcgctt aagataaatt caggacacta aatagtacag
     5941 gaagtagact aaaactttta aaagttttaa gtctatctat ttggcaagtt taaaaaataa
     6001 aaattcggta tcaaaaaact gttaccgata acatttttga aagcgttgtc ttttgcttct
     6061 ctatcactta tagtgatgac gtcatattaa aacacaagga ggaaatcaat ggatacagct
     6121 gcaatttatc atcgaccgga aagtgaatat gcatttctat atacgaatac aagatttcgt
     6181 attcggcttc gtactaggaa aaatgatatc aaaaaagtat atgtgctatg tggagatcca
     6241 tataccataa caactgaaaa atggtatcaa aaacaacgac caatgaaaaa atgtctaagt
     6301 accaatgttc atgattattg ggaaattgaa gtgacgagcg aaacaagtcg cttacagtat
     6361 gcgttccatg ttgttgggga agataacacg gactgttttt acggtgatca aggaattttt
     6421 ccttatcgag aaaatgtgat tactgaacca aatttttatt ttcgaatacc ttattttcat
     6481 caaatcgatc gattcactat tccagaatgg gtaaaagaaa cagtatggta tcaaattttt
     6541 cccgaaagat ttgcaaacgg ggacaaagca aatgaccccg aacacacttt accttgggga
     6601 agtaaagatc cagaccgaga agactttttc gggggagatt tgcaaggtgt gatggatcat
     6661 ttggattact tgacggacct tggcatcaat ggcatctatt tttgtcccat atttaaagcc
     6721 acatccaatc ataaatacga tacgattgat tattatgagg tggatcccgc ttttggcgat
     6781 aaaaaacttt taaaaaactt gattgatgaa gcacataaaa gagggatacg tatcatgttc
     6841 gatgctgtat tcaatcatat gggtgtccat tcaccacagt ggcaagacgt tctcaaaaac
     6901 ggagaaaaat cgatctataa agactggttc cacatccgtt ttttcccagt agattcttat
     6961 cagatgactg acttaccaga aactgctgaa aatatcccat atgatacttt tgcttttact
     7021 ccattcatgc cgaaactaaa tacagcaaat ccagaagtgc aaaaatattt gctggatatc
     7081 gcaacttatt ggataaaaga attcgatatt gatggatggc gtcttgatgt tgctaatgaa
     7141 gtcgatcatc atttttggaa aaaattcaga gaagcagtta cagaaatcaa accagacatc
     7201 tatatccttg gagagatttg gcattcctct caagcttggc tgcaagggga tgaattccat
     7261 gcagtgatga attatgcatt tacagattcg attaaagatt attttgcaaa aaagaaaatc
     7321 acagcttcac aaatggtcag tggaatgaat catcagcaaa tgctttacag agatcaggtg
     7381 aacgaaggaa cattcaactt attagattct catgatactg ccaggatctt gaccctttgc
     7441 caaggtaaca aagaattgat gaagtctgtg atggccttta tgtttcttca aaaagggtct
     7501 ccttgtatct attacgggac agaaataggg atgactggtg aagatgaccc ggattgccgc
     7561 aaatgtatga tatgggagaa ggaggaacaa gatctagagc tattcggttt tataaaagaa
     7621 cttgtttctc taaggaaaca gttgtctaag attatttctg aaggttctac tcagtggcta
     7681 atagtaaatg atcgtgaaga taagctctat ttcacacgga aactagaggg acagatcatc
     7741 tatgtttact ttaatcagtc aaaagaacct tgggtagtag aacaggaaaa tgaggtgatt
     7801 cttagtcaaa attgtcagtt gttagaagac gggaaagcag agatacgaca atacggattt
     7861 ttgatcatgt gttgaaaact gttaccggta acaacaagaa agcgtttgca aatgaagatt
     7921 tgtgcccatt ataataaggg tgtaacagca cgttgaatac aaaggaggaa atgaagatga
     7981 aaaaaagaag tgtgaaacag ttgggtttag ggttaatctc tttaggaatc ttaggcggat
     8041 tagcggcttg tggcaatggt aactcttcag cagaagggga agccgatgaa ggaaaaacgt
     8101 taacagtttc cgttgattct ggatataaag attatataaa cgaaataaaa aacactttcg
     8161 aaaaagaaaa cgatgtgaaa atcaagctag tagaaaagga catgttcgat cagttggaat
     8221 cattagcgtt agatgggcct gctggaaagg gacctgacgt tatgatggca gcatatgacc
     8281 ggatcggtgc gttaggacag caaggacatc tggcggaagt aaaactggga aatgaagcag
     8341 cttacgatga aacagacaaa gcccaagtga cttatgatgg aaaaatctac ggagaacctg
     8401 cagtaatcga gacccttgtt ttgtactata acaaagattt agtcgataca gcaccatcaa
     8461 cgtttaaaga tttggagaat ctatccaaag acagtcgttt tgcttttgag tcagaagcag
     8521 gaaaaaatac aggattttta gctaaatgga ctgactttta ttattcctat ggtttaattg
     8581 caggttacgg tggctatgta tttggtgatg atggcacaaa tccttcagat atcggtttga
     8641 ataatgcagg tgctgtagaa ggaatctctt atgcaacaga ttggttcaaa aatgtttggc
     8701 caaagggaat gcaagacata aaaagtgcag gagacttcgc cagccagcaa ttcatgtcca
     8761 ataaaactgc agcaatcatt gacggtccat ggcaagcgca aacatacaaa gaaaacaata
     8821 tcaattatgg tgtagccaaa attcctactc taaataatgg acagccttat caaccgtttg
     8881 gcggaggaaa aggatgggta gtcagcaatt atgctaaaaa caaagacctc tctcaaaaat
     8941 ggcttgatta tgtaacgaat caggaaaatc aagaaaaatt ctttgaaatg gtaaatgaaa
     9001 tccctgcaaa ccaacaagct agggaaacag caaaggctaa aaacgatgaa ttaacaacag
     9061 ctgtgattga acaatatgaa acagctcaag cgatgccaaa catcccagaa atgggcgaag
     9121 tttggacagg tgcagaaaac ttgatgttcg atgcagcatc aggttcaaaa acaccaaaag
     9181 aatccgcaga tgaagcagta aaaacaatca gtgaagccat agaacaaaaa tactgattaa
     9241 aatgataacc gtccattttt ataaaaaatg gaacaggtgc ttcagataaa agtggcaaga
     9301 gcgagatgga aaattatctt gctcttgttg ccattatctt aaaagcagtt tgcggtttag
     9361 caagtttaga gggggcattc agatgcagca agataaaaca agcaatatca acgaaaaaaa
     9421 aatcacgctt tgttcactta ttccagggat ggggcagttt gtaaatggcc aagcttttaa
     9481 aggtatcgtt tttttcgcca tattcgtcct attcattggt cagatgcttc tgggcggatt
     9541 tcatgcattg gtaggattgg ttactttggg aagtatacca atggaagatc attcattatt
     9601 tattttgatt gaaggcactt tgcaattcat cattacaatc attttcttga ttttttacgg
     9661 tttgaatctt ttagacgctt atcgagtagc gaagatgcgt caagagggca aaaaaatcaa
     9721 tcggacgatg aaggaagtca tacataatat aggtgatgaa ggttttcctt atctattgac
     9781 acttcccgct tatcttctca tgatttttac gatcattttc ccagtcctag ttacattgtt
     9841 cactgcattt actaattatg acttcaatca tattccacca gccagtttaa ttgattgggt
     9901 aggggctaaa aactttttta gtatcttctt tttaagctca tatcgtaata ctttttttgc
     9961 agtcttttcg tggaccgtga tttggacttt atgtgcaaca actttgcaaa tcacccttgg
    10021 tattctcaca gcggttgttg ctcaccagaa atttattaaa ttcaaacgta ttttcggtat
    10081 cattttctta ttaccatggg cggtcccagc ttttattact atcatgagtt tttcgaatat
    10141 attcaacgat agcgtaggtg ccatcaatac tcaagtgatt cctttcataa accagcttcc
    10201 ttttgtagat atcagtgcaa tctcttggaa aacggacccg aactggacga aagtagcaat
    10261 tatcttagtg cagggctggt taggatttcc ctatatctat gtgatggtta caggaatctt
    10321 acaatcgatt tcagaagact tatatgaagc agcaaaaatc gacggagcaa atgcatggca
    10381 gcgcttttca aacatcacgc ttcctgctgt cttcctagtg gcagcgccaa catttattac
    10441 acagtatacg gggaatttca ataatttctc catgatctat ctgtttaaca atggcggtcc
    10501 cggaagcgta ggcggaggag ctggttctac tgatattctt atttcctgga tctataaact
    10561 tacgactgga acttctccgc agtattctat ggcttcagca atcacgttga ttatctccgt
    10621 catcgttatc tgtgtgtcat tgctggtatt taaaaagaca aatgcgttca atatggggga
    10681 ttaacaatga aaaaatttca ttcgagtgct cgattccaaa aacgactttc tcactttttt
    10741 acttactttt ttcttttaat actgtcagtc attatcatct atccgctttt gatcacagca
    10801 agtactgcct tcaagtcagg aaatattgca gcttttagtt tagatctttc aagtgactgg
    10861 actttaggaa actttactcg gctattcagt gaaacacttt acgggacttg gtataaaaat
    10921 acattgatca tttctgtcct aacaatgatc gtacaagtga ctgtagtaac tttggcgggc
    10981 tatacttata gtcgctatcg ttttatcggc cgcaaatcaa gtttgatgtt ctttttagtg
    11041 attcagatgg taccgactat ggcagcattg actgcctttt atgtcatggc tttcttatta
    11101 gaagcgctcg ataagtactg gttcttgaca atgatctata taggcggtgg aattccaatg
    11161 aatacttggc tgatgaaagg atactttgac actgtaccaa tagatttaga tgaatctgct
    11221 aaacttgatg gggcagggca tttcaggatt tttttccaga ttgtattacc gcttgtcaaa
    11281 ccgatgctcg ccgttcaagc attatgggct tttatgggac cttttgggga tttcatgtta
    11341 gctaagttcc tgcttcgaac acctgaaaat cagactgttg ctgtcggctt acaaacattt
    11401 atttctgatg tgacaaacca aagagtctct ctttttgctg ctggggcaat tttgattgca
    11461 ctaccgattt gtctattatt cttcttttta caaaagaact ttgttaccgg cctgacatct
    11521 ggcggaacaa aaggatagga gcagagtatc atgcaaacat ttccgttaaa ctatttttcc
    11581 gcattacgta cgccaactca agtgtttgcg ggacgcaaac tattatcatg gcctaaattt
    11641 ttcttgatat ttgtattttt agtttccttg atggtcatgc ctgtgactct tttttatgcc
    11701 aatcagatcc aagcgattcc attagaacaa tttctatccg ttcattcgct gatcgatgaa
    11761 caaggaaccc aaaaattttc tgaacttgaa ttatctgaaa ctgggttaca agcatcgcaa
    11821 caaacgatag ctgtgacccc tgaaattttg gtaggtgtgt ctttatctga gaaacaacaa
    11881 tccgatcatg gaacttttat tgattttgag aaggaacaat gggtgatcca acaaaaggat
    11941 aaatctggta tacgccgcta tacgatgaat tatagcccat ctttccaacc agattctgta
    12001 cgtacgcccg aagatttcca acgattttta gaaagagaat tttatgccag taatcgtcca
    12061 acaatcattt tatcttatag tttaagtctt ggtcttgtcc tatttgtcat gacgagtctc
    12121 attttattcg gggcttcctt tttcttgtgg atgacaagaa aaagccaact ttcatccatt
    12181 catacattca aagagagtgc aaatttgatg ctgaatgtta tgggaatagg ttccatggca
    12241 gcagcagttg tagggtttat ccactttgac tttatcttga tgctaagtgt ccaaacaacg
    12301 ataactgtct tgctgctttt atgggttttt gtcaaaacaa gattcaaaga taagcgtgtc
    12361 gaatgacaag tttaagcgat ttatgcgaca atgaatgtga aaaaaatgaa aaataaagta
    12421 gtttttgaaa aatttctgaa aaaattaaac ttcttagttg attcttttct tttttagtat
    12481 taacgtaata gggtagtttt tttaaggagg aaaatcatgt tagagaaaac tttatacaat
    12541 caattatttt cacgttcttt ctcgctccct gtcgaagtaa attattggga cggtacaacg
    12601 aaacaatacg gagatacgga cactcctccc caaatcaaaa tcaatatcca tgaagaaatc
    12661 ccaatgaaag agattacaag taacgcatct ttggcactag gagaagccta tatggatcat
    12721 cgaatcgaga tcgagggaag tatccaagca ttgatcaacg atgcttatca gcaagcaaac
    12781 agctttttac gaggaaaaga ttatctaaaa tggctgccaa agaaagaaaa acatacgaaa
    12841 caaaaaaaca aagaagatat ccatgcgcat tatgacttag gtaacgactt ttataaactt
    12901 tggctagatc caactttgac ttattcttgt gcttatttca atacacctga agatacgttg
    12961 gaacaagcac aaatcaataa agtccatcat atcttagaca aactctttat caaagaaggc
    13021 gaaacgttgc ttgatatcgg ttgtggctgg ggaacattga tgttcactgc taccaaagag
    13081 tataatgtca aggcaactgg gatcacacta agtgaagaac aatacgattt gattaccaaa
    13141 agaataaaag aagaacattt agaagataaa tgccgtgtat tattaatgga ttaccgcgaa
    13201 ctaaaaggcg aaacgtttga tcatatcaca agtgtaggta tgttcgaaca tgtaggttct
    13261 gaaaatcttg aaggttattt caaagtggta aaagatttat tgaaaccaaa aggaacagct
    13321 ttgatccatg gaatcagccg acaacaaggc ggtgcgacaa atgcttggat caataaatat
    13381 attttccctg gcggttatgt ccctggtgtt actgaattag ttgataatat tacaaaaaac
    13441 gatttgcaac tgattgattt ggaaagctta cgtcgtgact accaattgac attagaacat
    13501 tggacaaaga attttcatca agtccaagat caagtcacga aagaaatggg tgattccttc
    13561 tatcgaatgt gggatctata tttacaagct tgtgcagctt catttgaatc aagtaatatc
    13621 gatgtgattc aatatttgct tgttcaacct ggaaacaatg acatcccaat gcatcgtgca
    13681 taattgaggc aaaagtactg tttaaagact atttaaaaag aagctaagtt ttatgatgct
    13741 ccagaagtga acttttttgt ctaacttcta ggattgtctc taaagcttag tttctttttc
    13801 tttatatttt cataataaaa gtttggttct ttaattttta aacatgacag aaaatttttt
    13861 cttcgttata cttgtaatag aatactattt tataacaaag gggatcgaat caatgaaaaa
    13921 aactgttatt tatactagtt tatttgcagc tttgttttta gctgcttgtc aatcaaatac
    13981 caatcaaggc acatctggga atacgtcttc cagtagcgaa cagcgctctt cacaggtatc
    14041 ggactcctca acaaaagcaa gcacatcttc gtctagccag acaaaaagtt ctagtgaagc
    14101 aagtactcag tcagatatca aagtgtctgc agctcgtgcg atcgaattgt tccaagataa
    14161 atacccagat actgcaatta ctagtttgga attagatagt gattggggat cttacttcta
    14221 caagatagaa ggtgtcgatg atcaaaaaga atacgaagca agaattgatg ctcaaaatga
    14281 aaaaatggag acagagaatc cagaaactct agatcgagat gaacaaaatg gtcaaaagaa
    14341 aaaagaagat ggtctggatg ttgataaatt gatcacgatt gaggaagcag caaaaatcgc
    14401 cgtcgacaaa gtaggaagcg gtacggcaac agattgggat ttggataaag atttaggtac
    14461 tacttattgg gaagtcaaag taaaagacgg caatcaaagt acatcagtca agatcaatag
    14521 ccaaacaggc gaagtactgt caactgaaca agatgattaa gaatttcttc ggaaaacaag
    14581 agttgatttt tcttttagtc atgatacact agacaaaata acttgtttga aggagcgatt
    14641 tacttgagtt tagaaacgac ctttgccaat catatctttg ccaatccttt gatgaatgca
    14701 tctggtgtcc actgcatgac tacccaggaa ttagatgaac tggctcattc agaagcagga
    14761 gcatttatca ctaagagctg tacgatcaat gaacgaaaag gaaatcccga accacgctat
    14821 tttgatgttc ctttagggag tatcaattct atgggacttc caaacttagg tttttcttat
    14881 tatctagagt atgctcttgc gtatgaaaaa gtgcaggaaa accaaaatca accgttattt
    14941 ttttccattg ccgggatgag tgtccaggaa aacttggaaa tgcttgagaa aatagaaaaa
    15001 agtgggttta atgggatcac tgaattaaat ttatcttgtc ccaatgtgcc tggaaaacca
    15061 caactagctt acgattttga ggccacttat gaaacactga aagaagtatt cagcatcttt
    15121 tctaagccgc taggaatcaa attgcctcct tattttgatt ttgctcattt tgatcagatg
    15181 gctgatatct tgaatcagtt tccattgacc tatgtcaatg ctatcaacag tgtgggcaat
    15241 ggtttgtata tcgatacaga acaagaagca gtagtcatca aaccaaaaga agggttcggc
    15301 ggcattggcg gagagtatat caagccgact gctttggcga atgttcgcgc tttttataca
    15361 cgtttgaaac cagaaatcca aatcattggt acaggtggga ttcggacagg acaagatgca
    15421 tttgaacatc tgctatgtgg cgcaagcatg ttacagattg gtaccgagct tcataaagaa
    15481 gggccggaaa ttttttctag aatcataaaa gaattaacgc agattatgtc agaaaaagga
    15541 tatacgtcaa ttgacgaatt taaaggcaaa ttacgcacca tttcatagca aagagacgaa
    15601 ggtcatgatt gatcttcgtc tttttttata aaaaacactc gtctgttcat tcctgtgcaa
    15661 gcgtactcat tgtatgttag acgaacttgt gggaaaataa cagaggcaaa aaggaggata
    15721 atatgaatga tgcaacagaa ttagcaaaag agcttaaaga aaaagaattt agttttcaag
    15781 aactagttac ggatatacag aaaaaaataa agaagcaaaa tcatgaattg aatgcgtttg
    15841 tcgcgcttga accattcgag agtaattccc acgagctatt gattccccag tcagaaaaag
    15901 ggccattggc tggtatccca tttcctttaa aaatgctcgg tcaagaaaaa aagggatggc
    15961 tagctacttc aggatctcgt ttatttgaaa cacaccgagc ttctcataca tctaattatg
    16021 tgcggcaagc agaagcgata ggtctagttc cttttggaca aaccaatgcg ccagagtttg
    16081 gtttcaaaaa tattacagac ccagtcatct atggcccagc aagaaaccct tggaaccttg
    16141 aatgtacacc gggcggatca agtggaggag ctgctgctgc agtttcatca ggaatcgttc
    16201 ctttagctgg tgctagtgat ggcggtggtt ctattagaat acccgcttcg ttttgcggat
    16261 tgattgggct gaaaccttcg cgagggacga tgccagttgg tccatatgct tggagaggat
    16321 ggcaaggagc tgctattgat ttcggcttga cagtttctat gagagacacg gaggctttat
    16381 tttatgggat gagaagtatc cattccggtg cgccatatca agtatcgcca gttgagtggc
    16441 aaacacatcc aagaaaaaaa cggttgaaga tagctttttg tgttgattca ccgatccact
    16501 cacagattag tgaagaggca gtacttgctg taaaaaaagc tgtagatttc cttggaaaag
    16561 aagggcatga tttgacagag attccttacc cactagatgg tcgtaaactg atcacgtctt
    16621 attatcagat gaatggcgca gaaacagcag caatgatccg ctcgattgaa acaggcatcc
    16681 agcgagctgt cagaaaagaa gaacttgaac ccatttcttg gactttgttc caatatggtc
    16741 agaagctctc tgccgctact tacatcgatt ctctgcatgt atgggatgaa gcagcaattg
    16801 tcatggaaaa cctacatcaa gaatacgatc tattcctttc accaacgact gcatttaccg
    16861 cccctaaaat cagcgaagat ttgcaaagtg atgcgataag agcacagatg gaacacatca
    16921 gtgattgttc tgaagtagaa ggtttagacg ttgtttcttc catgtttgag gagagcttga
    16981 agatcacacc ttatacacag ttggctaatc tgactggaca accagcaatc agcttgccta
    17041 ctcatttgac aaaagaaaac atgcctctag gaatccaatt tatggctgcc agaggaagag
    17101 aagatttgct atttcaaatc ggctatctat ttgaagcaaa tcatcagttc catttaccaa
    17161 atccatggaa atagcagaaa caaggagaag aagaatgaag aaaaaaagat tgtttgctct
    17221 aataactagt gcctttttta ttttaagcgc ttgcggtaca gctgccgcta atcaggaaga
    17281 taacagctgg agtaatgtag aaaaaaagaa agaattagta gtagcgactt ctggcacact
    17341 gtttcccgca tcttattata atgatcaaaa tgaattagtt ggttatgatg tagatttggc
    17401 aaaagaggtg gctaaacggt taaatgtcaa aatctccttt aaagaataca acgtggatgg
    17461 acaaatcact tctgtttcaa aaagagaatc agattttgct gcgaacgatt ttggtatcac
    17521 aaaagaaaga gaaaaaaaat tctccctttc agagactttc aaatattcat ttgacagtat
    17581 gatcgtacga aaaaaagatg attcaggaat ccactcactt gaagatctta aagggaaaaa
    17641 agcagctggc gaacccaata ctagctatat gcgtttagcg gaaaaacacg gagctaaatt
    17701 agtcacatat gacaatgcca cgaatgatca gtatttaagc gatgtagcaa atggtcgaac
    17761 agatgttatt ctaaatgatt attatctgca gaaaatggcc gtatctgctt taccagatat
    17821 ccctgtaaaa atactggaga acgtttattt caatgaaaac gaaagtggtt ttttattgaa
    17881 agaaggaaat gatgctctga aaaagaaaat cgatggcgta ttaaaagaga tgaaagaaga
    17941 tgggacgaca aagaaaatat ctgaaaaata ttttcaaact gatgtttcag tcaaaccgaa
    18001 agaaacgatg gctgagtcgg tgagtgagtg atgtactttc ccctaattga tttttccctc
    18061 atgtggactt cacttccttt cgtactgcaa ggcttggtat acacattagg gattggcttc
    18121 gtgtcgtttc ttttaggaaa tttggtaggg ttgcttttga cagttttagg tcttttggat
    18181 tggctgcctc tgaatgtatt tattcgtttt tatttatcgt tctttagagg cattcccgca
    18241 ctagtcctgc tgtttctgtt atatttcggg ttaccttatc aattaagtgc cttaacggct
    18301 tcagtcatct gctttacgat taccagcagt gcatttattg gtgaaatcta tcgaggatca
    18361 ctagcaggcg tttcttcagg tcagtgggac gctggtttag cttctggttt ttctttcttt
    18421 ggggtattga aatatatcgt tttgccgcaa gcttttcgaa tctcagtacc ggcactcagt
    18481 aacgtagcta tggatctagt gaaaggtact tcgttagcag caacaatcac agtgccagaa
    18541 atatttcaaa aagcaaagat cataggcggt cggacattcg attatttttc tctctatatt
    18601 ttagttgcac tgatttattg ggtcatttgt atcgggatcg aaaaagtgca acagtattta
    18661 gaaaaacgtt ctatccatca gtcaatctga aaaatagaaa tggtgctagc gtggctggca
    18721 ccatttctat tttttgtttc ttgtatattc tgcctagtat tttaaaacca tttcatgtaa
    18781 aatgtaaatc gaaaatatgt tcggaggtag aaaacatgaa gaccccgcta accatcgaaa
    18841 tagaatcctc gttataccaa tattgcatcg agcaaggcgg cttggttgta gaagaagtca
    18901 cgatgcctga tgaccaagga attgtagata cgcttagctg cttgacaaaa tttgatggaa
    18961 gccgagaatg gcgatgctat gagctgaaag tgacaaaagc agattttcgc tctacagcca
    19021 aactgtcatt tgttggacat tataattatt ttgttttacc aagtaaacta tatgaagaag
    19081 taaaaactga aatccctaca ggaataggtg ttttagtgta tcgtccgtac acggttgaag
    19141 aggagatgcc tgcctctggt acatttctga ttgcgaaaaa agcagcaaag aaagagcttg
    19201 ctgtaccgga agaagctttg acaaaccgct tcatggcttc tttattcagg gaagtaagaa
    19261 aagcaaagca aatggagcga ggcgtaaaat attttccaaa agatcaattg tacaaagaat
    19321 tgaaaagacg ttcagaaaat caagatccat tttctaacga acgatattat gaacggtttt
    19381 tagaggaaat agctagtcag gcaatcaatg agttgcaaga agaagttatc gcgcttcagc
    19441 atgattatga atatttgagg caacaacagc agatccgtcg gttgcctaca gagccattgg
    19501 agtgaaaaat atgtactatc cttatttaag aggaaaacaa tttgacctat tagcattaaa
    19561 agaagcactg tcgcgaggat tgctctccaa taaaatccaa ccagtgatcg aaccagtacg
    19621 tgattccgct acgcttaaaa atgtgatcga attatttcaa aaaaagcacc atccaatcgc
    19681 tgtgatccaa aatccccaag taggacaatt caaattattc gatcagcatg tgcacagttg
    19741 gacagtcaaa gaaaatagct ctgtcgtccc tgcacagatc ttaacgccag aaaatctcag
    19801 tgaagtactg gattcacctc cagcgtttct tgttttcgat ggacagcact acccaaaaga
    19861 tacagaggtg tggaaacagt tggcaggagt tgattcgaaa tttttgatac cagatactag
    19921 tcgctttcgc atctggctgc ccgaaaacaa aattgtgatc cgtgactctt ttcaaacgag
    19981 aaagcatgtt gaaagttatg cagataaaac agatgacttt tttagtgatg actatctttt
    20041 tttccacgca gacggatata ttggattttc tgatttcacg atcgaaggta gccgttattt
    20101 cgataaaggg ttccctagcc gagcgcttgc tatccatcta acgtatattg atgcttacgg
    20161 caatatacgc gtgaaacatt ttgtctctga cagtaacgac tctgctaaag accaagcatt
    20221 gaagtttctt gaagcaggag ataaaatgaa aaaatggatc atgcgtcacc atcatcaact
    20281 cttgattact tcaggaatga ttgaattatt ggctctttac cagcaacaaa aattccctgg
    20341 actgggagtg ttgaaaaagt ggtcacttat gcatcatttg gaacttatta gtcacctgct
    20401 agatcatcca aaagattggc ttaaatatta ttcaaaagtt caggaactat atgaagtgat
    20461 ccaatgatta gataatgtaa atgaaaaagg aatagaaaag aggaaactgg atgtatattc
    20521 ggcaaacaag gaaagaagaa gtagaagaag tggtagaaat cattaatgcc gcagcggatt
    20581 tcattgcttc gcaaggctct cctcaatggc aaaacgggca gaaaccaaca aaagaaacga
    20641 ttttgaacga tattgaaaga aaagaaagct atgtattaat agtggaagat caaattgctg
    20701 gaacagctgc attagtctca ggcgtggatc ccgtttacac aaaaatagat ggtagttgga
    20761 aagaatcaac tggtccttat ttatcgattc atcgtgtcgc tttagcagaa agtactcgag
    20821 gaaaaaaact agcaaaaccg ttcttagaac atttgttgac gattgcagcc agcaaaaata
    20881 tccaagatat ccgtatcgat acttatccag ctaatgaacc gatggaaaag accatctatt
    20941 ctgctggatt cacttatcaa gggatgattg aatttccttt tgaacacggc gaaagaaaag
    21001 catatcaaat aatcatcaat tgactataaa atcatctgag caaagataaa gacattaaca
    21061 ctactgaaaa aaacaatcat atgatagcca agaggttgta acagaagtgg ccagcttcaa
    21121 aaaacaagaa agaatttccg aaaatgcttc tcaaattttt gtggaatttt agcgtatttt
    21181 acgaaatagc tgctttctga aactcccttc atcgagaggt cgtgacatag cttttgtcac
    21241 gatctctcgg cttatcctac gagtattatt cgatacattt atatcaataa tccttttttt
    21301 attttatctg cagcttcttg gaacgctcca tcgtttgaca cgactaaaag tgaggggata
    21361 gctttcaaac gtttcaactg tgctaaatct tctacataaa ccgccagtct ggctgaaatc
    21421 tgttcagatg aatctccacg ttttttcagg cgcgattgca atgtttcttt agagatcgta
    21481 ataaagatag gacggactac cgtcccgaaa tactcatata aatgccaaaa accatttgct
    21541 gtcaaaacgg cgtaacaatt tcctactgtc agtttctttt taatttctgc tttactggaa
    21601 ccatatttat gttgagcata ttggtcccac tctgcaaact catttccctt gatcatctga
    21661 ttgaatgtat tgtcagaaac aaaataataa tcctcttgat ctttttcccc aggtcttggt
    21721 gatcgattcg tgtaagtaat gatcttttgt tccggctgaa aaatagcttc tgcgagtgaa
    21781 gtttttccag aaccagaagg accgataaat acatagcaat aattcattgt catttctcct
    21841 ttgatgattg aacaagtgaa aaagctttgg aaaatatttc caaagctttt aaaagtaact
    21901 ttgctttttt gtattttata ccagcaagcc agtcacaata gcacttaaaa agctgactaa
    21961 agtcgctgtt agcaaaattt tcaatccgtg ttttgcgaca agatttcctt tttcttcatt
    22021 taatcccttc atcgcaccag aaatgatccc gatagaagag aagttggcaa atgaaacgag
    22081 gaagacagat ataattgctg ttgtacgctc agtaaaatgg aaattaccat ttgctaattc
    22141 tgtcatagca acaaattcat ttgtaaccag tttagtcgcc atgatactac cggcatccac
    22201 tgcttctttc catgggattc cgctgatgaa ggcaagaggg gcaaagatgt aacctaggat
    22261 ctcttggaaa gtgatgccga atatcccgtg gaagatagca ttgatcatgg caatcaaagc
    22321 aacaaaaccg ataagcatag cagcaaccgt gatcgccaca tggaaaccat caagaatgta
    22381 ttctcccaac atttggaaaa atgtctgttc tttgttttct tcaatgatca gctcatcttc
    22441 tttttcttct aatacatagg gatttacaat tgaagccaga atataaccgc caaataagtt
    22501 caaaacaagg gcagtgatca catatttaga atcgatcagt gccatataag atccgacgat
    22561 cgacatagaa actgtagaca tagccgatac actcatggtg aacaaacgtt tctcagatag
    22621 gaagggtaat tcctttttta tggaaataaa aacttccgat tgtcctaaaa tagcagaagc
    22681 tactccatta taagattcca atttgcccat cccgttgatt ttacttaatc ctagaccaac
    22741 agctttcata aataatggaa gaatcttgat atagcgtaga ataccgataa tagcagaaat
    22801 aaatacaatt ggcatcaata cggaaagaaa gaaaggtgca cttcctttat ttgctacacc
    22861 ggcaaaaaca aagtcaacac cttgcccagc aaaagcaaga agatgatcaa acatttttgc
    22921 caatacagaa acaaccgctg taccaaaagt tgtacgtaat aatacgaaac caagaacaag
    22981 ctgtaaaacg aacatcacaa ttagtggttt caatttgatt gcttttttgt tactgctgat
    23041 caaaaaagca agccctaatg tgacaaataa accaataata cctaataaat aactcaaata
    23101 atcactccta ttccaaattc cgttttattt tacctgtttt gcaaataaaa tactaatctt
    23161 tagtgaaatt ttcattactt ttttagagtg aaacgcattc attttccatt agaaaataaa
    23221 gcgaacaaaa taaaaggttt aaaaagtgta acgtcttagt aaagtctata aaaaaacgaa
    23281 actatcgatt aagctaatag tttcgttttc aacatttata agtaaacttt ttctattggt
    23341 aatatagatt ttcggaaggg tagacaggtg cgcaggtaat atcgattcct agttttttca
    23401 atgtttgttc atcattttta ttgaccatca cagtagagtg ggcttgtgct ccctcaagtt
    23461 cagctagttt atcataagca acttgagcga ttggattcgt tactgcactg attgataaag
    23521 cgatcaagac ttcattgatg cttagtgcat ccaaagagga atgaagtgtc tttgatttca
    23581 atccttgtat tgtttcaaga actaatggtg acaataaagg aatgtcatct gcgatatggg
    23641 ctaaatgttt tactgcattt aagataactg ccgctgacgc atccatcaat gaacttgtac
    23701 gcccggtaat gatgacttca tctggtaatt cgattgcaat cacagcagaa ggatcttttg
    23761 taggactttg cgcttgaatc ttatctgcat agtcatgggc agggactacc acgtttcggt
    23821 cttcaggacg taaaccgact tcttccataa tcagtttgat tcgattaact gcttcttcgt
    23881 cgatcaatcc ttttttgaag tcgcattctg tttggaaata acgacggatg atttcttgtt
    23941 tagaggcttc ttggacaacg gtatcatcca cgatcccaaa tcctacacga ttcacaccca
    24001 tgtcagtagg agattgataa acagattctt taccagtaat tttttcaata atacgtttga
    24061 ttacaggaaa tgtctcaaca tcccgattat agttcacagc tactttattg taggcatcaa
    24121 agtggaagga atcgatcata ttgacatctt ttagatcgac tgtggctgct tcataagcaa
    24181 tgttcagcgg atgtttcaac ggaacattcc atactgggaa agtttcgaac ttggagtaac
    24241 cagctgcatg tcctttttgg ctttcatgat acagttggtt caagcaagta gcgagctttc
    24301 cacttcctgg gccaggagct gtcactacaa cgattggttt agttgtttca atatactcat
    24361 tttttccaaa accatcttct gagacgattt tttctacgtt gactggatag tcttcgattt
    24421 ctgagtgttt atagaccttg atgtttcgtc tttctagttt attgataaag actttcgtgg
    24481 caggctgtcc actatagcga gtgataacga cactgttgat taataagcca taaccacgta
    24541 attcatcgat taaccgcaag atatccatat cataagtaat cccataatca ccacggatct
    24601 tattacgttc aatatctcca gcatagacgc aaatcagtat ttcagcttga tctttcaatt
    24661 tttgcagcaa tttgattttc gcatcttctt caaatcctgg aagtacacgt ttcgcatgct
    24721 tgtcgtcaac taacttccca ccaaattcta aatataattt gtcatagtgg ttcactcgtt
    24781 ccaaaatata cgctgactgc tcttcaatat atttttctga atcaaaacca atttttttca
    24841 ctaggttccc tccttaagta ctaaagcatt ttttcatttt tttgaggatt cgtcaactgg
    24901 aagagcgaaa ttcctcaaaa aaacaaagaa aatggtttaa accaacttgt ctagatagtt
    24961 attcattggt atgatagttt ttgataagga aggtgctaca tgaaaaaaag acagaaaaag
    25021 aaaaacgcat ataaacacta tatccgttcg atttttaccg gttatgaaag aatgcttgaa
    25081 gatcccgaat tggaacaatt gacgtttact tatctaaacg aagaaacgca gctaactcgt
    25141 gatgagcatc agcgcattca tttcacaaca agagatctgc ctagtaaata aaaaataaga
    25201 taaaggtcat cggattcgat tctgtcctat cagatggaaa gactagttga tcgaaaaaaa
    25261 ggctgtacca aattttggtg cagcctgttt tatggagcat tgcccgctct tttttataac
    25321 aattttttac agaaaatggt actattgaag tattaataag tgaaaaacag atgagtaaga
    25381 ggaggcaagt agtatgaaag agccagatac aaatgcaaat tatgagccag acgaactaga
    25441 ggagacgaac gcggatcagg atacaggaca agaatcgtct tctttttacg atatcaaaga
    25501 aaaagcaagc atgaatcttt cccaattatt agaagactta caggcttttg aaaaagctgt
    25561 ccgcgaagaa gatatccctg agatctaccg tttatacaat ggccgattga atgaagaatt
    25621 aaaaagaagc tcaaatgaaa atcacgagat cgatcaattg ctgatgcgta agatccatga
    25681 tcgtttttta caggaatttc cttttatgga acaagtagaa aacatctcgc caacgatgag
    25741 ctattacaaa atcggcactt attaccatga tcggcccacg gtcggtattg atgcaagtct
    25801 gccagaaatt tttgttttgc ctaagatcga tgaagagtgg gagaaatatt cccaaccgga
    25861 gtcaacggac tatgacaaaa aaatcaatga gctagacgca aaggtgatca ctgctcagac
    25921 agaaattgaa cgtttagatg aacaaataaa agaaatcaat cgacaaatga ctgatttgaa
    25981 cgacaataaa ggattcttga atcgtaaaaa aatcgaggaa gaaattcaag aattagagaa
    26041 gaagaagcaa gtactatcta atgaaaaatt aggctggctt ccttatattg agacgcctga
    26101 aacaatccag cagcaaaaag aagcattgaa gcaagaagca agagcagacc agcttcgtgc
    26161 agccatcgta gaaaaagaac aaaggcaaat cagacgctat ttcggtagca aagagggatt
    26221 cggtcaagca atccatgaat tcttgatgaa ttatcttggc aatgagaatc ctaataatca
    26281 atctaatgaa ggaggttccg agtatgaatg aacacgatga agcagaatgg acaaaaaatg
    26341 aagaacaaac agaagatcat gaagaaacga acacttcatt ggaagaacaa acaagtaatc
    26401 ctctggatga cacagaagaa atccctatgc tagaggaaac aaaaatacat gaaaaagaaa
    26461 aagtaactcc gacgaatacg aatactgcag aaaacaccca tgcttacgaa ataactgaaa
    26521 atcccgggta tttagaagga aaagagcaag atcatcaaga tatcgtcagt ttgattgaaa
    26581 cagcggaagc agaattagcg gatttacgta tgagaaaaga tttgatcgaa tcagattttc
    26641 cacaattatt agaatcatat gatcgtttta tattgaatga agaaccaatc tccacgatcg
    26701 gaaagacaag cctgatcgaa tacttcacgt atgaattact gaaaccgttg aatgatattg
    26761 gtctggaacg gtcaaattca gcttatgaca catggaaaac aagacatttt tcgatcaatt
    26821 atacgttgaa tggacaaaac gaattagtct tttcctttgt attaccgacc atcgatcaac
    26881 gataccaagt ggaatcgatg agtctcctaa acgtatcacc agagacaatg gaaatcaatg
    26941 tccaagatga ccgggtgctt tctttgatcc gacactggtc agtggatcga gtattctctg
    27001 cagggcagat cacgatattc aatcacaaat tgaatcaatt attagcccat gcaagaacac
    27061 ttggttttac cgttaatcag acattactag acaatacgaa gccacttcgt ttgaatcttc
    27121 agagcgaatt tgaattgacg aatgaagtat tggatgatat ttttatcgtg gcaatgaaaa
    27181 atccatccta cgatatggaa aagatcaacg aaactactta tcaagtacga ttagataaag
    27241 gacagtcttt aacgataaca acaaacgaaa aagatcaaac agtcttgtcg atctcatctg
    27301 gagactaccc acgatctgtc attgatttct ttatcaatta cgaattcttg gtcccattaa
    27361 tggtgagaaa gtcctgattg gataagttgg attaccagat gtcattcaga taattctgtt
    27421 ccaaaggact tgcggcagtt atccagaata attgtcacaa gtcttttctg tatgtactaa
    27481 atggacgtga ataacttgct gatgaaggag ggatttcatt aggaatgtcc acaaataaaa
    27541 tagcacagct tagcttattg tctgctgctt gtgtagcagg taggatcgct tttcaattta
    27601 ttccgaattt ccagccaatg actgctattt ttttatttat agtactttac ttaagcttaa
    27661 aagatgcgtt aattgtcatg agtctttcta ttgcgatcag cagtttttat tttggtgttg
    27721 gaccatgggt catcggccaa tggatcagct atactgttgt tctgagtttg ttttcaatcg
    27781 tctgcttgag aaaaacagtg caaaggaatc tatggttaaa gggagtctgt ttttttctgg
    27841 cgggcatggt ttacggaata ggtatgaccg tttttgatac gctgttatat cgtttgcctc
    27901 aaccttggat ctattacctt caaggggtgt cgtttgattt gatgcacagt ataggcaatg
    27961 ttgtgttctt tcttgttttt ctgccagtcg tcaaacgttt ttacaatcat tcaggaggaa
    28021 aaaatgaaaa aaataattta tagtttgctt gcaatgagtg tcagtatact cattttgaca
    28081 agttgcagta caacctatac acaatcggct tccaaccaaa aggaggaagt aaaaacatcg
    28141 caaaaagcaa caatcacgtt acaagaaaat ggtcaaaact tttcggaaaa agaagtggtc
    28201 ttcaaaaaag gcgatgattt actgagtatt ttgaaacgaa attttgaagt caaagaagat
    28261 aacggcttca tcacttcgct tgaagggcac tcacaggatg agaaaaatca actctattgg
    28321 atgtttactg tggacgggaa aagtgctact actggtgcca aagagatcaa attgaatgat
    28381 ggacaatcgg tcattttcaa tttgtctaaa ctataagttt cataaaaaac tgttaaaggg
    28441 cgaatattgc cataattcag tgagttcagt tgatgaaaga aggaaaataa catggaaaat
    28501 tttaaaaaaa gcggaatgtt ttccgtaatt gctgctctag gggctaatat tctagttgct
    28561 atcagcaaat ttgtcggcta tgcaatcagt ggcagtgccg ccatgctaaa cgaaagcatc
    28621 cacagtatcg ttgattgcag caatcagatt ttgctgttat tcggtgataa acaagccaat
    28681 aaaggacaga gcgagttgca tcaatttggt gaaggacgag caaaatactt tttcagtaca
    28741 atcgtcgcaa tgatgctttt cttcggcggt ggggcacttg gggtgatgga agcttttgaa
    28801 aagttgacgc atccatccca tgaagtgggt aatccgatca ttgttatagc aatactgtta
    28861 tttggtttgg taatcgaagg aagttcgtta cgcgtggcaa tgaaagaaat caaagaattg
    28921 aatactgaaa aactttccac gatgcgcttt ttgcgcgaaa gtcgacacag tgaaatactg
    28981 attattttta cggaagactt gtgtgctgta atcggtttgg tcttggcttt agcaggaaca
    29041 atattgactt atgcaaccgg gattgctgca tttgatgcga tcagcggatt gctgatcggt
    29101 ttcttgctga tgggagccgc cattttccta gcaaaagaat tttatagtct gatcatcggt
    29161 gaaagcgtga cagcaagcga tttatcaaaa atcaagttag cttttgaacg tcctgagatc
    29221 agtcggctga tcgatataaa aacagttcat ttaagtccaa cggagatttt agttgcagca
    29281 aaagcagata tcgttggtaa caaagagaat gaagcttact cggtcatcaa tgatattgaa
    29341 aaaaatatgc gtcagtctct tcctgacaag aagatgtata tatacattga aacagataaa
    29401 tatgacccaa attattctcg taaataaaac gtttattcat gaattgaagc aaaaatgaga
    29461 aaaatataat cttgttttta aataaaacgg ctacaattta gcgaatttat gttacaatga
    29521 gtccgctgtt tatttgttgt aattaaagga gatattttaa tgaacaaaaa gaaaagtgcg
    29581 ccatggcttc aagctttgca tgcagtgatg ccactttgta taagctatat accagtagga
    29641 ttagcttgcg gagtattgtt gcaaaaagtt ggatttgatc ctctcttatc aggactgtta
    29701 tcgattctgg tcttttccgg tggcgcgcag tttttagttg catctatgct tacgacgcaa
    29761 gcatcatttg caacgacatt attgatggtt ttctttttag aattacgcta tacattatta
    29821 ggagcaagtc ttgccggttt tatgaaaaaa gaaaagcgga cgtttttggc tgtcttttct
    29881 caatcactga atgatgaaaa ctatgcggtg aactacttaa agttttcaac tgacccttcc
    29941 tggaataaaa gaaaggcttt atatgtcaat tggttctcaa tgactgcatg ggccggtagc
    30001 aatatgatcg gtaatttttt cggctcggtg attcgagtag atgctgacct tgtccatttt
    30061 gctttgacgg ccatgttcat tttcatgttt atcatgcaga tgaaaagcgc actactgatc
    30121 tttacaggtt tattctcagg cgtattaggg gtcgtattca tggtattatt ccaaaatact
    30181 ttcggattga ttgcagcaac agtcattgct tcatttgccg gatttatgct ggagaaggca
    30241 ttcaaaaaag aaaaagctaa gaaactaaga gaccgcggta aagatgtcaa tgagccgctc
    30301 ttcaacttcc cggaacagga ggccaatcac catgactaaa ctatatttgc tacttttagt
    30361 tgcttgtctg ttcatcgtgg catatatccc gcgcttgttc ccgatgcttt attttactca
    30421 tcgaaaagtg ccatcctggt tcagtgactg gatgaaatat gtaccggtgg cattatttgc
    30481 ggcgttagct tttaaagatg ttttcattac tcatgaacat ctggatattg cttggaatat
    30541 caaaatagct gcaatgattc tagtagcagg cgtggcttac aaaacacgtt cgatggcgtt
    30601 gtcagttttg acaggacttg cgtctgtctt tttgctgtct atgctttaaa ctgttttatt
    30661 ttattaaaaa cagagattgc gacataagtt gttcaaactg atctatccga actgcatttg
    30721 caacagtcaa ttctggatgc aagtttattc gtagggataa aaaagattag tgttggctta
    30781 tgatgcaagc tcttttttat tttaggaatt agagagggat ttgtccttct atgatttttt
    30841 gctataatag ggaagatgtt tattttatga aagaatagtt gagggaattt aaatgaataa
    30901 aaaattgatg caaggaacat tttggctaac ctttgctaat cttctatgta aagttttagg
    30961 ggtagtctat ttgattccct ggctaagtat gatggggaac aatcaagatg gtatgcttgc
    31021 tacgacattg tataatgttg gctatcttcc ttatggattg ttcctgatgc ttggtacagt
    31081 cggatttcca aatgcgattg caaaaaaagt agctgtagcc acaaaagaag gagataacgc
    31141 tgcttgtcgg ctgattttta gaagtacgat caatatcatg tttgtcatcg gtatcgcctc
    31201 tgctgcactg atgtatttat ttgctccttt actagccgga atcagtccga ttgctaacgt
    31261 taataatggg atactagcta tcagaagttt atgcccatct ttgattgcta tcccaattct
    31321 tagtgcaatg agaggatatt tccaaggaaa aaatgatctg cggccttatg gaacttcttt
    31381 gattatcgaa caggctgtgc gagtgatcgt tatcttggct ggtacttatt acttgcgcgt
    31441 attgactgac ggaaccatat tagaagctgt attgatcagt acagtcgctt ctttcttcgg
    31501 tgggttagct gcgattatcc atatgtatat cgtcggacgc agaaaagact tcttcgaact
    31561 taaagatttt ttgatttcca gccgttattt cgaaagagaa aaccgatctg cctctgtctc
    31621 tatcattcgg gaaacactac cgtttatttt tgtcggctca gtcattacta tcgttcaaat
    31681 gatcgaccaa gtaacgatga aaccactgct tcactttttc cgtccagaga tcgcagatca
    31741 gcaactggaa ttcttattca gccgtgcttc tgtcaatcca aataaattga cattgatcct
    31801 gatctccatg gtaggaacag tagctataag cagcttgcca atattaagca cgatgaagaa
    31861 aaaagatcgg atacaaatcg aaaaaacagt aggagatagt ttttcgattg cgttattgat
    31921 tcttcttcct tcattgactg gaatgtcttt attagctgga ccactataca cattgttttt
    31981 tggctatgat cctgaaagtg tcggatattt ccaaatggct ttgctagctt ctttgttttt
    32041 ctctttattc acgatcctat ctacgatgct tcaatctttg aatcaccatc gttttgcgat
    32101 ccgattaact gtagaagcaa ttatcctgaa agtcatcttc caagtaatcg gattaggatt
    32161 agttggcgga tacgggatga gcttatcaaa tggcttagca ttcggtattg tctttgttcg
    32221 cggctatcac tttatgtgca aagaataccg aatagcacct ttagcaaaga tcagcaattt
    32281 tttcttaaaa actttccgct ccaccctgat tatgcttgca gtatgttttg ccgttttctt
    32341 tttgctgaat cagcgattgt caatggaatc aaaaagctat gcaatgattt attgtgtggc
    32401 tgtaggaagt attggcggct tagtattttt attttctcaa ttcggaaaaa atgggtttcg
    32461 aatgttgaaa aatgttaaac atcatagcag aagcaaagaa taggttaact aatcgttttt
    32521 tgctgtcact tgaaaaacgg caactcgtat gattacgctt gtcgtttttc tattttccaa
    32581 ttttagtttc atatctaatc ttacttatga tatagttata aggtctggaa aaattctttg
    32641 aaacgaggaa attaaacaca tgaaacttgc agtagttaca gatagttcgg catatctgcc
    32701 ggaacgtatc aagaaccatc ccgacttatt cgttattcca atccctttga ttatggatgg
    32761 gaaaatttat aacgaaggaa tcgatatcga agcggatgaa tattatggac ttttaaaaaa
    32821 cagtaaagat tttcctacta cttcacaacc tgtcgtaggg gaagttttgg gtttatatga
    32881 ggaattgaaa gagaaagggt atgacacgat tttaagtatc catctttctt cagggatttc
    32941 tggctttgtc aacacattat ttgcgatgaa agatgacatc agcggagtaa cggtcattcc
    33001 ttatgactct aagatcacta gtatgccgat gggacacatg gtagaagctg ccttagactt
    33061 aaatgatgaa ggtcagtccc ttgatgaaat acttgcacat atcgatcgaa tccgagataa
    33121 tacctatgct tatctgattg tagatgattt gaacaattta gtacgaggag gacgtttgac
    33181 aaatggtgca gcgctgattg gcagcttatt aaaaatcaaa ccgatcctga cattttctga
    33241 agggaaaatt gttttattcg aaaaaatccg ttcaagtaaa aaagcatttg ctcgagctga
    33301 agaaattatt ggtagaagaa ataacgaaat acaaccgcca gtcaaacttt acgtgataca
    33361 tgcaaataat ttagaagtag cgattgcgga gaaagagaaa ttgcagaaaa aatatccaaa
    33421 tgcagaaatc gaaattggtc atttcggacc agtcattggt acacatttag gtgaaaaagc
    33481 catcggcatt gccatttcag cgcaataatt ccttttattc aaaaaacata ccaaaaata
//
LOCUS       DAZULO010000021        32178 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000021 DAZULO010000000
VERSION     DAZULO010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.021"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(407..766)
                     /locus_tag="ACG8OA_000999"
     CDS             complement(407..766)
                     /locus_tag="ACG8OA_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFS4975829.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="ACG8OA_001000"
     CDS             complement(796..1137)
                     /locus_tag="ACG8OA_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975830.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="ACG8OA_001001"
     CDS             complement(1134..1808)
                     /locus_tag="ACG8OA_001001"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS4975831.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="ACG8OA_001002"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="ACG8OA_001002"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFS4975832.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="ACG8OA_001003"
     CDS             complement(4322..5512)
                     /locus_tag="ACG8OA_001003"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS4975833.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="ACG8OA_001004"
     CDS             complement(5533..6060)
                     /locus_tag="ACG8OA_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFS4975834.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="ACG8OA_001005"
     CDS             complement(6107..8896)
                     /locus_tag="ACG8OA_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFS4975835.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="ACG8OA_001006"
     CDS             complement(9043..9243)
                     /locus_tag="ACG8OA_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4975836.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="ACG8OA_001007"
     CDS             complement(9696..10016)
                     /locus_tag="ACG8OA_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975837.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="ACG8OA_001008"
     CDS             complement(10519..11637)
                     /locus_tag="ACG8OA_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975838.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            11870..11992
                     /locus_tag="ACG8OA_001009"
     CDS             11870..11992
                     /locus_tag="ACG8OA_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975839.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            12108..13415
                     /locus_tag="ACG8OA_001010"
     CDS             12108..13415
                     /locus_tag="ACG8OA_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFS4975840.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="ACG8OA_001011"
     CDS             13536..14735
                     /locus_tag="ACG8OA_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS4975841.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="ACG8OA_001012"
     CDS             complement(14762..16030)
                     /locus_tag="ACG8OA_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFS4975842.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     regulatory      complement(16086..16152)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(16200..16841)
                     /locus_tag="ACG8OA_001013"
     CDS             complement(16200..16841)
                     /locus_tag="ACG8OA_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFS4975843.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="ACG8OA_001014"
     CDS             complement(17250..17753)
                     /locus_tag="ACG8OA_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4975844.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="ACG8OA_001015"
     CDS             complement(17876..18640)
                     /locus_tag="ACG8OA_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFS4975845.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="ACG8OA_001016"
     CDS             18748..19023
                     /locus_tag="ACG8OA_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFS4975846.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="ACG8OA_001017"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="ACG8OA_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFS4975847.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="ACG8OA_001018"
     CDS             complement(20579..20980)
                     /locus_tag="ACG8OA_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4975848.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="ACG8OA_001019"
     CDS             complement(20994..21803)
                     /locus_tag="ACG8OA_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4975849.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="ACG8OA_001020"
     CDS             complement(21790..22680)
                     /locus_tag="ACG8OA_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4975850.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="ACG8OA_001021"
                     /pseudo
     CDS             complement(22693..23172)
                     /locus_tag="ACG8OA_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(23348..24289)
                     /locus_tag="ACG8OA_001022"
     CDS             complement(23348..24289)
                     /locus_tag="ACG8OA_001022"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFS4975851.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="ACG8OA_001023"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="ACG8OA_001023"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS4975852.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="ACG8OA_001024"
     CDS             complement(25274..26470)
                     /locus_tag="ACG8OA_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4975853.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="ACG8OA_001025"
     CDS             complement(26488..27216)
                     /locus_tag="ACG8OA_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4975854.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="ACG8OA_001026"
     CDS             complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="ACG8OA_001026"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS4975855.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28628..29086)
                     /locus_tag="ACG8OA_001027"
     CDS             complement(28628..29086)
                     /locus_tag="ACG8OA_001027"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFS4975856.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29083..30546)
                     /locus_tag="ACG8OA_001028"
     CDS             complement(29083..30546)
                     /locus_tag="ACG8OA_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS4975857.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="ACG8OA_001029"
     CDS             complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="ACG8OA_001029"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS4975858.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31602..>32178
                     /locus_tag="ACG8OA_001030"
     CDS             31602..>32178
                     /locus_tag="ACG8OA_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4975859.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         9385 a         6921 c         5511 g        10361 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgccatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcagcaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttatcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt
    27541 tattattagt tttagataac ttttgcagtt ttatactttt tttctataaa atttgacaaa
    27601 cattttttta aataactaat tattttttga atctgtcact cagacaatcc tttctcttac
    27661 ttgttaacat aaatagaagt tgctgtttct tctaatagtg tacttaattc tttaatattt
    27721 tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggt
    27781 gctactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat
    27841 tgttttgcaa aacgcaatgt atcttgaaat agtgcagcgc tgactcccgc acttaaaaaa
    27901 ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga
    27961 ctataacaag tatcttgccc atatccttcc acaaatctca tattcactgg tacttctact
    28021 ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc aatcacttta
    28081 tgcggtttta cttttgcata gtcaagagaa gacggatcgc caatagttgc atcgtaagaa
    28141 aggagctcta aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat ccgttctata
    28201 actatttgtt tttgcacatt aatctctact ggttcatcaa cgtcatagta caataaaaat
    28261 ttacaagcat ccgcacccac ttcttttaac cttttcactg accaactatt cagtaaatct
    28321 ggtagtcggc ctggcatttt cgcatcatag ccggtttttt cataagctag caacaaacca
    28381 cattctttct ctcgaagcgc tgctgcaggc agaccatatt ctggatcgag taaaatagcg
    28441 gaagcatacg gagtcaggta ctgagaaacc atctgtttaa attggatgat atcttctgat
    28501 gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgttggtc gatagctagc
    28561 gcgctaataa ttccagcttc atccgtcaat cgcttcaaac gttcctgttt ttctatactc
    28621 attttcttca tatatcttca cctatttctt tagttaatat tgtagctatt tctatttttg
    28681 attttgcagc tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag
    28741 acaataattt caaatgagtc gttcctgctt ctgaatctgg gataaataac gcaaaaataa
    28801 aatcaactga ctgtctatct aaactgttcc attcaattcc ttttttcagt gtgacgataa
    28861 tcaaactagg ttcattgatt gtttgagact tcgcatgagg gatagcaaaa cctgacatta
    28921 ttcctgtagt accttctttt tctctttcca gaagtttttg atatacttct ttttcgtcat
    28981 gagcgattcc taaacagaat gtttgctttg ctaaaaactt aaatgcttct tcttgattat
    29041 ctaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata aacctcctgc
    29101 tcttccttct tcgataactg tacgatttgt tgctttatta tccctcttct tgtctttatc
    29161 agttactctt ttcttttgga tcaaacctag tatcactcca ccaacgatcg aaccaataag
    29221 aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa aaccgccatg
    29281 tggggctggt acttggacac caaacagata ggtcaagaca gcagcaattg atgagcctaa
    29341 catcatcgtt ggtaatactc ggatcgggtc tttcgcagca aacggaatag ctccttccgt
    29401 aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat tagtatcaaa
    29461 atatttacca aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa tgcaagctgc
    29521 tgacgcacca gccataaaag tcgtgtttcc ttgtgtcaaa agagctgttc ctgttacata
    29581 tgccgcctta ttaaccggtc ctcccatatc aaacgcacac atacagccaa caatcacgcc
    29641 aagaacaagt ggattcgaat attcaaatcc agctaaaaag ctcatcattc cttcatttat
    29701 agccttcatt ggacctgcca gcaaagacat cacgatgcca ataaatgcta caccaataac
    29761 tggatataag acgattgcct tcaagccatc tagttgttta ggtaaaatta tcagtagctt
    29821 ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaatacctc ctaaaaatcc
    29881 agtaccgcca ttcatggcta cgattccacc aacaaatcca acgactaacc ctggacgttt
    29941 cgcgatccct tctgcaatat aagctgctaa aactggaacc atcattccca ttgctgcatc
    30001 gtcaactgct tttaccattg ccgctgtagc attgtattgt gcatgatctg gatcagaaga
    30061 atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg
    30121 caacatatga gagacaccgt tcatcaaatt tttatagatg ctacgaccaa aattagcacc
    30181 acttatcgct tctggttcta aagtatcagc agcttcatta tctgctaaga cagtaccttt
    30241 tcctgccaat gcttcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac
    30301 atcaatgact cgttttccca caaatctttc tgaatggaca tctttgtctg cggcaataat
    30361 gacagcatct gcctgtttga tttcttcagc cgttaattca ttctcgatcc caatttgtcc
    30421 atgggtctcc actttgatcg tgatgccttt tttcctagct gcttgctcta atgcctcttg
    30481 cgccatgtag gtgtgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt
    30541 tcccatcttt cgaacctctc ttccttgatt ttgatttgtt tacttaattc ctccacatcc
    30601 aaaaaatcag tcagtccttt acgaaaagca gtagagctgc ctgctgctat gcttttacgt
    30661 aacgtttcat caagcgatcg tcgattcatg tatcctgcta aaaaagtgcc taacatggca
    30721 tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctcg aatcaccctg
    30781 tctttcgtaa aaagaatcgc tcctttctca ccgagggata acaaaacatt ttctgcgcct
    30841 gactctacta gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg
    30901 aaccaagaag aaagttcttc ctcatttggt ttcagcaaaa acggtcgata gggtaaacaa
    30961 tccatgactt cttgatcact actatcaata atcaaacaaa tccgactgat ttctattaaa
    31021 acctttggag acagccctct tggtaaactc ccagaaacac aaagataatc ccctgcttgt
    31081 aatgctctta tttgatttaa aaaattatga acagctgtct gcggtatttc aggtccttga
    31141 ttcactaatt tgtattcttc tcccgtttga ttgacttgtg taaatacatt gatacgcgtc
    31201 ataccaggca cttcaataaa ctctgtttgg atttgtttct gctgtaaata gtcttctata
    31261 tactttcctg tgaacccagc agaaaaacct aatgccgtac ttgggaaatc tagcatcttt
    31321 aatactagtg acacattcac cccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg
    31381 tttaccataa aaggatgcat ctcatctgtt tcaataaata gatcaattgc caaattcatc
    31441 gtgcatgtgt agatagacat aaccaacgac ctccttgatt ctattatgaa atacgcgaaa
    31501 tagaaaatgt tttcttaatc gtcccaacac aaagaaaacc tttacattta taaactcgtc
    31561 gtgctttttt taactaactt ttggtgaaag aaggaaagaa tatgaatcac aaattaagtg
    31621 aaacagagca gtacctctgg aactttatcg aacacctcat cctagagatc cctaactatt
    31681 ccatcgtgaa actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga
    31741 aaaagaaagg ctatgaaggg tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca
    31801 agaacatcaa tttttcattt ttagataaag tagacaaagg aatccgccga gccattctaa
    31861 aaaatgaaca agaagtggta cgtacaatca atatgttgga aatcgggaac attgaagatg
    31921 caatacaaag aatcaaattt gctgaccggg tttttgtctt tgcccgagga ttttcagaaa
    31981 tgattggcca agaaatgctg gtaaaacttc aattaacggg aaaaaattgt cagatgcata
    32041 ctgatccaga aattatcaaa agcatcagtc ggaaactaac taagaaagac agcgttattt
    32101 ttgtttcttt gaacggtgag aaaacaaata aaaacatcaa aaataagatt tatgtcttta
    32161 tttttcaatt ttttaaat
//
LOCUS       DAZULO010000022        30661 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000022 DAZULO010000000
VERSION     DAZULO010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.023"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..299)
                     /locus_tag="ACG8OA_001031"
     CDS             complement(<1..299)
                     /locus_tag="ACG8OA_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS4975860.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGD"
     gene            complement(301..498)
                     /locus_tag="ACG8OA_001032"
     CDS             complement(301..498)
                     /locus_tag="ACG8OA_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS4975861.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(892..2619)
                     /locus_tag="ACG8OA_001033"
     CDS             complement(892..2619)
                     /locus_tag="ACG8OA_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFS4975862.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2612..3805)
                     /locus_tag="ACG8OA_001034"
     CDS             complement(2612..3805)
                     /locus_tag="ACG8OA_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4975863.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3992..4636)
                     /locus_tag="ACG8OA_001035"
     CDS             complement(3992..4636)
                     /locus_tag="ACG8OA_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4975864.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4730..4864)
                     /locus_tag="ACG8OA_001036"
     CDS             complement(4730..4864)
                     /locus_tag="ACG8OA_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFS4975865.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4866..6998)
                     /gene="feoB"
                     /locus_tag="ACG8OA_001037"
     CDS             complement(4866..6998)
                     /gene="feoB"
                     /locus_tag="ACG8OA_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFS4975866.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6995..7468)
                     /locus_tag="ACG8OA_001038"
     CDS             complement(6995..7468)
                     /locus_tag="ACG8OA_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFS4975867.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7869..8093
                     /gene="nrdH"
                     /locus_tag="ACG8OA_001039"
     CDS             7869..8093
                     /gene="nrdH"
                     /locus_tag="ACG8OA_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFS4975868.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8252..10411
                     /gene="nrdE"
                     /locus_tag="ACG8OA_001040"
     CDS             8252..10411
                     /gene="nrdE"
                     /locus_tag="ACG8OA_001040"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFS4975869.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10461..11426
                     /gene="nrdF"
                     /locus_tag="ACG8OA_001041"
     CDS             10461..11426
                     /gene="nrdF"
                     /locus_tag="ACG8OA_001041"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFS4975870.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11515..12654)
                     /locus_tag="ACG8OA_001042"
     CDS             complement(11515..12654)
                     /locus_tag="ACG8OA_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4975871.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12963..13676)
                     /locus_tag="ACG8OA_001043"
     CDS             complement(12963..13676)
                     /locus_tag="ACG8OA_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFS4975872.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13930..14631)
                     /gene="nagB"
                     /locus_tag="ACG8OA_001044"
     CDS             complement(13930..14631)
                     /gene="nagB"
                     /locus_tag="ACG8OA_001044"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFS4975873.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14865..16397
                     /locus_tag="ACG8OA_001045"
     CDS             14865..16397
                     /locus_tag="ACG8OA_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4975874.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16588..17790)
                     /locus_tag="ACG8OA_001046"
     CDS             complement(16588..17790)
                     /locus_tag="ACG8OA_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4975875.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17872..18561)
                     /locus_tag="ACG8OA_001047"
     CDS             complement(17872..18561)
                     /locus_tag="ACG8OA_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS4975876.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18667..19035)
                     /gene="rplL"
                     /locus_tag="ACG8OA_001048"
     CDS             complement(18667..19035)
                     /gene="rplL"
                     /locus_tag="ACG8OA_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFS4975877.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(19088..19591)
                     /gene="rplJ"
                     /locus_tag="ACG8OA_001049"
     CDS             complement(19088..19591)
                     /gene="rplJ"
                     /locus_tag="ACG8OA_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFS4975878.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19795..19922)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(20001..20690)
                     /gene="rplA"
                     /locus_tag="ACG8OA_001050"
     CDS             complement(20001..20690)
                     /gene="rplA"
                     /locus_tag="ACG8OA_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFS4975879.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20794..21216)
                     /gene="rplK"
                     /locus_tag="ACG8OA_001051"
     CDS             complement(20794..21216)
                     /gene="rplK"
                     /locus_tag="ACG8OA_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFS4975880.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21559..22227
                     /gene="sdaAB"
                     /locus_tag="ACG8OA_001052"
     CDS             21559..22227
                     /gene="sdaAB"
                     /locus_tag="ACG8OA_001052"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFS4975881.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22240..23112
                     /gene="sdaAA"
                     /locus_tag="ACG8OA_001053"
     CDS             22240..23112
                     /gene="sdaAA"
                     /locus_tag="ACG8OA_001053"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFS4975882.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23203..24303)
                     /locus_tag="ACG8OA_001054"
                     /pseudo
     CDS             complement(23203..24303)
                     /locus_tag="ACG8OA_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(24395..25282)
                     /locus_tag="ACG8OA_001055"
     CDS             complement(24395..25282)
                     /locus_tag="ACG8OA_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4975883.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(25359..25904)
                     /gene="nusG"
                     /locus_tag="ACG8OA_001056"
     CDS             complement(25359..25904)
                     /gene="nusG"
                     /locus_tag="ACG8OA_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFS4975884.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(26005..26175)
                     /gene="secE"
                     /locus_tag="ACG8OA_001057"
     CDS             complement(26005..26175)
                     /gene="secE"
                     /locus_tag="ACG8OA_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFS4975885.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26199..26351)
                     /gene="rpmG"
                     /locus_tag="ACG8OA_001058"
     CDS             complement(26199..26351)
                     /gene="rpmG"
                     /locus_tag="ACG8OA_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS4975886.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26513..27157
                     /gene="cbpA"
                     /locus_tag="ACG8OA_001059"
     CDS             26513..27157
                     /gene="cbpA"
                     /locus_tag="ACG8OA_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFS4975887.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27213..27716)
                     /locus_tag="ACG8OA_001060"
     CDS             complement(27213..27716)
                     /locus_tag="ACG8OA_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS4975888.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27729..27775)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28313..29278
                     /locus_tag="ACG8OA_001061"
     CDS             28313..29278
                     /locus_tag="ACG8OA_001061"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4975889.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29377..30549)
                     /locus_tag="ACG8OA_001062"
     CDS             complement(29377..30549)
                     /locus_tag="ACG8OA_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4975890.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30536..>30661)
                     /locus_tag="ACG8OA_001063"
     CDS             complement(30536..>30661)
                     /locus_tag="ACG8OA_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4975891.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         9356 a         6295 c         5417 g         9593 t
ORIGIN      
        1 ttatctccat aatctgctcg atataaacca tttattccag cagttcttcc aatttcactc
       61 aaatttctta ttaattcctc ttgatattca gtgaatctta ttttattagt ataagcagta
      121 aaatatagat aattcaccaa tctatttatt aaaggaccgg gatctaattc atatttttgt
      181 ttttctaatt ttaaatatac cttttgtaaa acctcaagaa tatcagaata tttaggatct
      241 ttacttatag atatatcatt atataattca tgaacaattt gcttagcatt ttttttcatt
      301 ttagcacttc cctggaattg ctccacctaa aaaaccacct atagacattc ctgcaataca
      361 agtagttgcc ttggcccaat cgaccgtaca tttattttta gtgcaataca ctccatttcc
      421 ataatatttt ccactatgag tggtaccccc atagataagt tgtgtctctt taatagacaa
      481 aatttttaaa tgtttcataa tctaatcacc cctaaattaa tgtttttaga ctattattct
      541 aacattcatt aaatttcaaa aaacaattcc ttcttgaaac gtagcaaaat aattcctgaa
      601 taagaattta atcactttta agtatataaa ctatgtatat tctttaatat tttttgagaa
      661 tgcacttcgt acataataaa ctttaatcac attagtcttt catacctacg atcgatctct
      721 ttgactcgca cccataaatt cataatagtt tgtcggcgtc tttggacgtt tcgaagatga
      781 gctacgcatt ctcttttttt atagacaaaa aagatagaga cgaacatgga gcttccaaat
      841 ttggatattt gatccaattt tggaagctcc atttatttcg tgtctgtctt tttatttttt
      901 ccagctttcg actaattctt ggttctcttt gatccagtct tttgctgctt gctgtggatc
      961 ttttccattg ttgatttcca acataacttt ttccatatct ttttctgacc aatggaaatt
     1021 gtctaatact ttgtaagctt ctggctggtc ttctttcaat ccttttcgaa ccatcgtatg
     1081 gatctgctct tcgcttccca ttccgttttc aggatcttct aagtatttca gatcatattt
     1141 ggcaaacatc caatgaggtg tccatcctgt aactacaatt ggttcgtgtt ttttgatcgc
     1201 ttgacctagt gcaacggtca ttgctcctga agaagatgtc tctactttcc agtctttcaa
     1261 gttgtcatat ttttggatcg tgttttcagc agccgtaact acaccagctc ctggctcgat
     1321 tcctatgatt ttcttacctg cctgatcggt taactcatcg atcgaattta catccatgta
     1381 ggctggcact gctaaaccaa cttttgctcc tgtcaaattg gcacctaggt cttcgacttg
     1441 atctttatat tgtgcatatt gtgaaccatg agttttaggt agccaagctg aaaccatggc
     1501 atctgactcg ccatttgaaa cagatttcca catgattgaa ttatctaatg gcgtcatcgt
     1561 tacatcatag cccatttgtt tcaatacttc accgactaca tttgttgaag caacttctgt
     1621 atcccattca acataagata agttgatacg ttttgtttct ttggcagatg aaaaactccc
     1681 tataccaatc gcacctaaga tcactactac tacagcacca atgatcagtc cttgacgttt
     1741 tttacttttc tgtgttgttg ctcctgcagc ttttttattg tttggctggt tcaaatgctg
     1801 tgtaaaacgg tcaatgatga ttgctaagat aactaatgca accccgttga caaatccatt
     1861 cccgacttgt gctctttgca atgctgataa tacgccgcgt cctaagccgg gtgcaccgat
     1921 catagaggca ataacgacca ttgaaagtga cagcattgtt gtttgattga tccctgccat
     1981 gatcgtactt ttagctaaag gcaattctag tttgaatagt ttttgacgac ttgtgctccc
     2041 gaatgaatca gaagcttcta ctaattcttt aggaacttgt cgaatcccta aattcgtaaa
     2101 acgaacggtt ggtggcagag caaagatgac tgaggcaaat acccctggaa ccatcccgat
     2161 accgaaaaat gcaactgctg gaatcaaata aacgaatcct ggcattgtct gcataaagtc
     2221 aaggatcggt ttgataatac tttgggcttt actgcttttt gccatcaaga tccctaatgg
     2281 taccccaatg atgatcgaga tcacactaga taaaagaaca agtgtgacag tattcatcaa
     2341 atcattccat agaccttggt tataaataaa ccatagaccg attaatgtga acaacgtaag
     2401 tcctggacgt ttcttagaaa taaagaaagc tgctaccgtc aataacaaga taaacaaagg
     2461 agctggaatc actaacagta aatttgtaat ccccgacatc actgcctgtc caatcgtttg
     2521 taaaaatgaa aataggccag aaaaagttgt cgtaaaccat tctgtgatat tttctaccca
     2581 gtttgccact ggtaattgat agttcaatag attattcatg ttcactcacc tcgcttgttt
     2641 ctgctaatgc ttcaatcaca ctacctcgga tgacgacacc ttttaatttt ccatcctcta
     2701 caactgcaag tggagtcggt gagtcataaa taatattgaa aatatcactt accaccatgt
     2761 ctttcccaat caccgtcaca ttttcatcta ggaagtcgat caatggacgt ttttgcttcc
     2821 tagcttctaa cgcttgatct gcagtgatga tccctttcag gtgtctcttt ctgtcaacag
     2881 ccatcagcat gctgacttct tcattacgca tccttgtcaa tgcaacgttt ggtccatcag
     2941 attcgatatt cgtcgtcaat gcaggtacca tgatgttttg agccgtaagc actttggaac
     3001 gatcgacttc ttccacaaat tcacgtacat aatcatttgc tggattggtc aatatttctt
     3061 cacctgttcc gatctgcatg atttcgccgt ctttcatcaa ggcgattctg tccccaatcc
     3121 gtaaagcttc attcaaatca tgggtaatga aaataatcgt tttttgaaca ttggcttgca
     3181 ggtccagtaa ttcgtcttgc atttctcgcc ggatcagcgg atcaagagcc gagaacgctt
     3241 cgtccatcag caagatctca ggatcatttg ctaaagctct ggctaggcca acccgttgtt
     3301 gcatcccacc agataactga ctaggatatt ggtctttaaa tgaaagcaaa ctagaatttt
     3361 ctaatgcttt ttctgctttt tcttgtcgtt cttctttagg tacaccacgg acttctaacc
     3421 cgtattctgt attttccaag atcgttcgat gtggaaacag accgaagttt tggaaaacca
     3481 tattgatttt atgacgacgg acttctcgca attcttcttt gcttaacttc gatacatctt
     3541 ctccatcaat atagatactg cccgaagttg gctcaatcag acgattcaat agacggatca
     3601 atgtggattt cccactcccg gaaagcccca tgatcacgaa gatttctcct tcttttacgt
     3661 caaaattcac gtcatacaca ccaaccgttg caccggtttt ctttaaaatc tccgttttcg
     3721 gctggtgatc attcatcata tctaaagcgg cttgtgtctt tttaccaaat atctttgtta
     3781 aatgttttac ttctactttt accaaacaaa ttctcctttt ttatgtaaat tcatttttct
     3841 ttctgaaaaa agtgaccaca ctatgttaac atcatgtagt cactttgtca aaaatgaaag
     3901 aaatttgctt gtccaaactg aaaaaaactt ggctaaatct ttgtaaataa aaggtttagc
     3961 caagttaata tttttttaat atttcacaca atcattctgc aagctgatgc tccgggtaaa
     4021 aaaagtttgt catacgctgt agtgtatatt catttccaat aaaatgaacg atgtcccctg
     4081 ccgtaaattt agcatagggg ccaggtgaaa caagcagttc ttcattatga gaaattgcga
     4141 taactgtggc gccagtcgcc tgccagatat tcaactcgct aattgaacgg tccagatgca
     4201 ttgctttttc tgataaagat aattcaaacg gaagtaaagg attcatctta tgtattcgct
     4261 tcgtttgatt gaccagctga tccaataatt gagaaaaatg attcagctca tcttgttgct
     4321 tctccacact tgccatgatt tctgcttttg tatcttggat ggattgtaca tcttgatatt
     4381 gcaataggaa atcttttgct ttttccttcg aagcaacaaa tgccccactt ccatgccgta
     4441 cttccatgat tccaagatct accagcacat tgatagcctt gcgggtagtc tcaggagaag
     4501 cattgtacgt actggcaagt gttgagcggg catggatctt ctcacccact ttatatcggt
     4561 tttcaacgat acgttcggct aaatcaacag caatctgctg gtagcgcggg cggacgattt
     4621 ttttctttcc agacattttc tttcctcttt ctttttttac tttccctcat cttactatgc
     4681 tctagcaaaa taagcaaaaa aggggagaat agcctcttgc aacgactgat catcgcttga
     4741 tctgttcctt ttttactggg caagctgttt gacagtcatc acaattctta ccggatttta
     4801 aacgagtata gacaattgcg cctgcacttc caaagattaa cacacttaat aagaatgtcg
     4861 ccatcctatt cctctccttc taatgacaat atagtataaa actggttctt ggattttggc
     4921 tgacggaaca aaacataaac gagtcctatc atcagtaaaa tagcaagata cgtccctaac
     4981 gctacctgtc caccctccaa taagtgtccc aactgataaa tcacaaaact aactaaataa
     5041 gctaaaccgc attgatagcc tatggcgatc caagtccatt tcttggcatt catttcccga
     5101 tggatagccc caattgccgc aaaacaaggc gcacaaagta aattgaaggc taacaatgaa
     5161 taagctgctg cgggagtata tgtgtgttgt agcacttgcc agatttctct gccattttcg
     5221 gatacttccg catgaccaaa caaaatacca aatgtgccaa taatattttc tttagcgacc
     5281 aatcctgtaa tcgctgcgac tgttccttgc cagtttcccc aaccaagcgg ctgaaaaaca
     5341 actgccagga ttcgcccgaa aaaggctaag atactttggt cttcagaaac aggttgagca
     5401 taaaaattat aagaagagct gaaccagatc acgatactag aaacaaagat aatcgttcct
     5461 gcttttttaa caaaagactt gctgcggtca taggtttgtc ttaggaccgc tgaccattga
     5521 ggaaaatgat atgcgggcaa ttccatgata aaaggagctg tatctccagc aaacaaacgt
     5581 gtttttttca agctgattcc agagaaaata attgcagtca taccaagaaa ataggcagaa
     5641 ggagctaccc aagattgatg aggaaaaaag gcccctgcta ctaaggcaat aatcggcaac
     5701 tttgcagaac aaggcatgaa ggttgtgacc atgatcgtca aacgacgatc ttgctcgttt
     5761 tcaatcgtgc gactggccat gacgcctggc actccacatc ctgtagcaat cagcatagga
     5821 atgaaggatt ttcctgaaag accgaatttc ctgaaaagtc gatccatgac aaaagcgatc
     5881 ctcgccatat atccgcagtc ttccagaaag ctcaagcata aaaacaatat aatcaattgc
     5941 ggtagaaatc ctaacactgc tcccacgcct gcgatgatgc cgttcaagat cagctcctgc
     6001 atccatcccg ctacttgcca ttcagctaac aatctgccaa catgttctgg taaccatgta
     6061 ccaaacaatg tgtcattgat ccaatctgtc cccattgttc ctactgtttg aatagccaga
     6121 taatagatca gccacatcac aaatgcgaag atcggcaagg ctaaccaacg attcgttgca
     6181 atctgatcga ttttgtcact catcgataat tggaaagaat catttgacac aacacagagt
     6241 gtacatagtt gggtaataaa ttcataacgt tcattgacca gaatcgtctc actatcgtcg
     6301 tccaatagtt tctctgttaa ctggatcagt tgctcgatct ctttttgctg agaaactgct
     6361 atctcgtaat cctctcttac cttctgatct cgttcaaaat atttcattgc ataccagcgt
     6421 ttcttgccgc tttgttctcc tagtacatct tcaatttcat gtaaagctgc ttctaaacgt
     6481 ggatcatatg tacagggaac aggaatagaa ggccgctttt ttgcagactc ctgaatcttt
     6541 tcgattagtt tttcgattcc ttgctttttc aatacgctga ttggtaaaac tggtacacca
     6601 agaccatagg aaagcttttc tagattgatt ttttttcctt cttttttgat gacatccatc
     6661 atattcagac caatgatcat aggaatgtct aaatccaata actgcgtagt taaataaaga
     6721 tttcgttcta aattggtagc atcaataatg ttgatgatca gttcaggctg ttcctgaacc
     6781 aaataatctc ttgcaatcac ttcttctggt gtataaggag acaaagaata gatgccgggt
     6841 aggtcctgaa tatgaatgtt cggatttttt ttcaacgatc cttctttttt ttctactgtt
     6901 acgcccggcc aattccctac atattgattc gaaccagtca agagattgaa caagctagtt
     6961 ttcccgctgt ttggatttcc gactaacgcg atattcattc tttttcctcc ctttgtatca
     7021 agattagttc ggcctctgac tttcgtaacg tcagttcata ccctcgcact gtcagttcga
     7081 ttggatctcc cagaggggct agttttcgga taactatttt tacatttcgc gtcaatccca
     7141 tatccattaa ccgtcgcttc actgcgcctt gtccgtggat tgctacgacg atcccagtct
     7201 ctcctgcttt taattgatca agagaagtcc agctttcaga ggaagcagtc tttttattga
     7261 caaagatttg acctagtatt tcccgactta ctgccactcg atgacttttt accagtaaaa
     7321 tggcattgtt gcctgataaa ttgacgacga cgattttact tccggcaacg atcccgatat
     7381 tttgcaaatg gcgttttgtt ttttcttctg tttcgattcg ttcgacttga taaacgaaat
     7441 tctgctttgc ctgcgctaag gtctgcatat tgattcctct cttgtgaaat aaatcatgta
     7501 ttaaaatcat tcattatatg ttctaatttt cttacgtgtt acgcaaataa atgagaatca
     7561 ttctcacata gaatactctc ggtaattgag aatgtcaatt atacttgtga tattatgagc
     7621 gaatctttag tattgataga taaaaatgag aaaaaaatat ttcttcatag ttttccacaa
     7681 ttttttctct attaaatttg ctattttccc acacgatact tgttttcagc tcttttttca
     7741 aaaacacaaa acttagtatt ccacagtttc cacaacacaa tatgtagtgt ttgttgtttg
     7801 acttttttac taagagtttt atactatcta ttatagataa taagaatgtg ttagaggagg
     7861 cactcaccat gaacgtaaaa atcttttcta aaaataattg tatgcaatgt aagatggtca
     7921 aacggttttt aacagaaaat caaattgctt ttgaagaagt caatatcgat gaacagcctg
     7981 aagcagtaga ctggttgaaa gcacaaggat tccaaagtgt tcctgtcatt acttctgatg
     8041 cagaaacagt tatcggattc cgtcctgatc agttgcgtaa acttgccagc taacatgact
     8101 aaaagaatca aaacgtgata catggatttg aagggcttga gacagcacca gtttttgtcg
     8161 caaacttcca aatccttttt taaagcttaa aaacaatgca atgatttgca atcaagcatc
     8221 atcaaatacg aaatgaaggt tgggcgtacg tatgagtctt aaaaatttac aagatgtcag
     8281 ttatttcaag ctgaataatg aaatcaatcg gccagtcaat ggtcaaatcc ctttgaataa
     8341 ggataaagaa gctttagttg ccttttttga ggaaaatgtc aaaccaaata cgatgacttt
     8401 tctatctatt atggaaaaaa tcaattactt gatcgatcaa gaatatatag aagcagaatt
     8461 cattcaattg tattcaccat catttattga aaagctatat caatttttgg acgagcaaca
     8521 tttttctttc aaatctttta tggctgctta caaattttat tctcaatatg cgttgaaaac
     8581 caacgacggt tccgcttatc tggaaactta tgaagatcga gtagccttca atgcgcttta
     8641 ctttgcgaac ggggatgaag aattagcact tgctcttgca gacgaaatga tccatcaacg
     8701 atatcagcca gctacacctt ctttcttgaa tgctggacgt aaacgtcggg gcgaattggt
     8761 gtcttgtttc ttgactcaag tcactgacga tatgaactct atcgggcgct caatcaactc
     8821 tgctttgcaa ttgtcccgta ttggtggcgg cgtagggatt actttatcaa atcttcgtga
     8881 agccggcgct cctatcaaag gatatgaggg tgctgcaagt ggtgtcgttc cagtcatgaa
     8941 attattcgaa gatagtttca gctattctaa tcaattaggt cagcgtcaag gagctggcgt
     9001 ggtttatttg aacgttttcc atccagacat tattgctttc ctttcagcaa aaaaagaaaa
     9061 tgcagatgaa aagattcgcg taaaaactct ttctcttggc gtgatcgtac ctgataaatt
     9121 ttatgaattg acacgtaaaa acgaagatat gtatttattt agtccatata gtgtcgaaaa
     9181 agaatatggt gtcccattct cttatgtaaa tattacagct gagtatgatc atctagttgc
     9241 aaatccaaac attcgcaaac aaaaaatcaa agcgcgtgat cttgaaaatg aaatctcaaa
     9301 attacaacaa gaatctggct atccttacgt aatcaatatt gatacagcga accgcgagaa
     9361 tccaatcgat ggcaagatca tcatgagtaa tttatgttca gagattttac aagttcaaac
     9421 gccttctgtg attaacggca aacaggaata tgaagtatta ggaacggata tctcatgtaa
     9481 cttaggctcg acaaatatcg tgaatttgat gcaaagccct aactttggtc aatctgtgcg
     9541 tacgatgact cgtgcattga catttattac agatgcatct gatatcgatg tcgtaccacc
     9601 gatccaaaac ggaaataagc tgaatcatac gattggatta ggtgccatgg gcttacacac
     9661 ttatcttgca aaagaacaaa tggaatatgg ctcagaggat tcattagact ttacaaatat
     9721 ttacttcatg ctgttgaatt attggacact tgttgaaagc aacaatatcg ctcgtgaaag
     9781 aaaacaagtt ttccataatt ttgaaaaatc agcttatgct gacggcactt atttcgaaaa
     9841 atacgtaact ggagaattcc agccaaaatc agaaaaagtc aaagcattgt ttgaaggcat
     9901 ttttatccca actgctgaag attggaatgc tttgaaacaa gcagtaatga aagatggact
     9961 gtatcatcaa aatcgcttgg cagtagcacc aaatggctca atttcatata tcaacgatac
    10021 gagtgcaagt attcatccta tcactcgcat gatcgaagaa cgccaagaga agaaaatcgg
    10081 taaaatttat tacccagcgc cttatctatc caatgagacg attccgtatt acacttctgc
    10141 ttatgatatg gacatgcgta aagtaatcga tgtttatgca atggcacaac aacacgtcga
    10201 tcaaggcatg agtttgactt tattcatgcg ttctgaaatc ccagaaggca tttatgaatg
    10261 gaagacaaca acgaagcaaa cgacacgtga tttgaatatt ttaagacatt acgctttcca
    10321 caaaggaatc aaatccattt attacgtacg tacctttact gatgatgcag aagaaatcgg
    10381 aagcaatcaa tgtgaaagct gcgtgatcta atatcataga tagattcgct tattcatatt
    10441 ttaattaaag gagcattctc atgtcagaaa catattatga agcaataaac tggaatgaaa
    10501 ttgaagatat cattgataaa tccacttggg agaagctgac agagcaattt tggctagata
    10561 cacgtatccc actttcaaat gacctagatg attggcgtac attgtctcaa ttggaaaaag
    10621 atacagtagg acatgtattt ggcggtttga cactgctaga tactgtccaa tctgaaagtg
    10681 gcatggatca gttgcgtaaa gatgtccgca ctccccatga agaagccgtt ttgaacaata
    10741 ttcaattcat ggaatccgtt catgccaaaa gttattcttc tatttttagt acactgaata
    10801 cgaaaaaaga aatcgaagaa atttttgact ggacaaatac gaatccttat ctgcaaaaaa
    10861 aagctgagcg tatcaatgaa atctacaaac acggtacacc gctagaaaag aaaattgcca
    10921 gcgtcttttt agaaacattc ttgttctatt caggttttta tactcctctt tattatttag
    10981 gaaacaacaa attggctaac gtagcagaga tcatcaaact aatcatccga gatgaatctg
    11041 tacatggaac ctacatcggt tacaaattcc aattaggatt caacgagtta cctgaagcag
    11101 aacaagaaac gttaaaagac tggatgtaca atcttcttta tgagctttac gaaaacgaag
    11161 aacgttatac agaagaatta tacgatccaa tcggctggac agaagaagtc aagacattcc
    11221 tacgctataa tgccaacaaa gcattgatga acctagggat ggatccactc tttccagata
    11281 ctgccaatga tgtcaaccca atcgtaatga acgggatttc aacaggcaca agcaaccatg
    11341 acttcttctc acaagtcgga aacggctatt tgttaggtca tgtagaagcg atgaaagacg
    11401 acgattattt gattggattg gaataaggat ttattttaat tcaaaaattt ttcgaaaaat
    11461 aaaaagagtt tagcgtacag atattttaaa tgtacactga actctttttt atgcttattc
    11521 acttgtttct tccagcactt ttggactgtc taacttttcc gaaacatgtt ccattcttct
    11581 caataaatag attttccgga tatttaatat tatcgtctta atcactgcat agacaggtat
    11641 gcaaatcagc atgcctaata ttcctgcgac acttcctgct ccaatcaata ccaagatgat
    11701 cgtcaaagga tggatattca ttgatttccc aaataacaac ggcttgatca aatttccatc
    11761 taattgttgg atcactaaaa tcgacaacgc catatacaaa gcttgaaatg gagagataaa
    11821 caaaccgaca atgattgctg gtaccgctcc aataaaaggg ccgacatacg ggataatatt
    11881 cgtgattccg caaatgaccg caagcaacaa gctataaggc tgatcgaata ttctcattgc
    11941 caaaaagctc attacaccaa taatcaaagc atctaagatc gtactgctga tataggcaga
    12001 taatgtttcg tttaattctt ttaccgtttg gcgtaactct ttacgaatcg ctttcggaaa
    12061 gaagtgagac aacgcatcaa taaatttgtg cccatccttg aacataaata gcaaaataaa
    12121 tggaacggta aataacaaaa taaaaaattg catcataaat gaaatgattt ttgagacact
    12181 tgcagataaa ctgactaata tgatattgaa caaattacta agagataaat ttgctgatgc
    12241 taattgttct tccaaattga aatttttaaa ctcttcccgc tgcgccaatt catttaacca
    12301 tttgcttgtt tcatctgcat acattggcaa gctctgggtc aattgaattg tctgttccat
    12361 taactgaggc actacattca tgacagttaa aacaactaaa atgattacga ccaaaaacgt
    12421 caataaaaaa ccgaacactc tcgggatccg atgcttttcg agaaaaacaa cgattggatc
    12481 gaacatataa tagataaaac cagcaacaag caatggtaaa aaaactgccg aaagcatttt
    12541 caatattgga ctaaaaatat tcggcatttg taaaataaag tacacgccga tgatcgtgag
    12601 tactagctcc actgtccaga aaaataattt tgattgttta aaacgtgatt gcatacatct
    12661 cctccaaggt cctttgatac ttctattcta tcatttaaca aagagttacg cgctgagatt
    12721 ttcatttgct taaaaaaaac aaaaaaaata aacatatttt aatgagtaga cgagtactta
    12781 aacaaaaaca cggtttggag tagtgacaaa aattatactg cagtcccttt ttccaaataa
    12841 acggtgttca aaagcttgca tcttcgtaat aagcccagcc aaacgaaaaa tataaggaac
    12901 tatttttatt tggctgggct tattacttga tgtaggacat ctattttaca acctcagatc
    12961 atttattgcg caatactgac taaatgcaac tttgtgatat ttttgtgtac ggacaactcc
    13021 tcgatcacat cggcataagt gagccctttt ggaagatcaa tcgtatagat atttttgtac
    13081 actcggtagt catcaacgaa ttttacgtca aaattcacat cttcgattac gatatttttc
    13141 tcttcaaaat aatcggtaag aaaatctttg gtttcttttc tatggataaa ttggatttct
    13201 aactttttag tcgtaggtac atggatcacg cgtttcacta ctgtgagtgc aatagcaatc
    13261 ccgataaaac tgatgatcgc aatggaatag taacccatgc cgatggcgat ccctaatcca
    13321 gcaattgccc ataaagaagc ggcagtcgta agtcccgtca ccgattgttt cgtgacgatg
    13381 atcgttcccg cgcctaagaa accgacacca ctcaccactt gagcaatcag ccttgcttca
    13441 tctgaacgaa tcgttccagc aagctgtgga tactctaaag catctcttaa tgcaccacta
    13501 gcgatctcaa cttggatcaa tgcaatgata catgctccca tacaaactaa aatatgcgta
    13561 cgcatacctg ctggacgatt tttatattgt cgttcaaaac cgattgctcc gccaaatagc
    13621 atagcgatcc ctaaacgtaa aataatctct ggaattgtca ataccatatc attcatctaa
    13681 acaccctcca gtccaatata tcatgtctga aaggagcaga caaaaaaagt gcaattttta
    13741 cctcttacga aaaaaggact gtgacaaaat ttttgtcata gtccttttta tgaataaacg
    13801 gtgttcaatc agctgaccct cggtattact caacaaacgg caaaatatgg ggagctattt
    13861 ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttagcctac
    13921 tattgaaaat tacaacttac ttgctgcttc ttcatcgatg atcacaacaa cattgtcgtg
    13981 attttgcaat acacttgctg gcaaatgatt cgttacaggt ccttcaatca tccctttgat
    14041 tgcatcagct ttgctttcgc caaatgccat caagacgatt tctttacttt tcataattga
    14101 accgattccc attgaaacag cttttgttgg tacatcttct gctttatcga agaatcgttt
    14161 gtttgcattg attgttgatt ctgttaagtc aactactgaa gtcgttccgt caaatgaagc
    14221 accaggttca ttgaaaccga tatgtccatt ttgcccgatt cctaagattt ggatatcgat
    14281 tggatgtgca tcgatgattt gatcatactt tttgcatgct gcttccagat cttctgcttt
    14341 tccatctggt acaaacgttt ctttgaatgg ttttttatca aataattgga catccatgaa
    14401 atgacggtag ctttgttcat ctgaaccgcc tagtccaacg tactcatcta agtttacaga
    14461 agtcatttca ctgaaatcca aatcgcttga agtcatttct ttatataaag taactggtgt
    14521 gcttcctgtt gccaatccta atacttttgc acctttgttc ataccttctt tgatgatttc
    14581 aaatgctttt tttccgcctt cttcagcgtt ttttacacga atgatttcca tagtgattgc
    14641 tcctttatat tttggtatag cccaattata gcattctttt tttcttttta caataattag
    14701 tatagaccaa attgcatttt tttcattttc ttgacaataa ataggaatcc gtgttaggtt
    14761 tcataagatg gttcaatcaa acaatccatg ttgcatgaaa accaagcaag tgattcactg
    14821 tggacgcttt atgtctgcag tttttttatg aaaaaggagt tattatgagt atactgacat
    14881 taaacaacat tacccagcaa tttggcgaca aaatcctcta tgaaggtgtc gatctgcaat
    14941 taaatgctgg cgaacatctt gggctgattg gacaaaacgg agccggtaaa agtacattga
    15001 ttaaaatcat cactggtgag atcttaccag atgacggaca gatccattgg caaaaaaata
    15061 ttcacaaagg ttacctcgat caatatgtgg aagtcgatga aacattaaca atcgaagaat
    15121 ttctaaaaac tgcgtacgca aaagaatacg aaaaggaaga aaaaattgct tttctttacc
    15181 aaacatatag cgagaccttc gatgattcct tgctggaaaa agctgggaaa ctccaaacgg
    15241 aattagacca aggagtgttt tatcaaatcg acaccttagt tgctgagatg agcagcgggt
    15301 tggggatcga tgtcttaggt ttagattcac tgttgaaaaa tctaagtggt ggacaacgtt
    15361 caaaggtgat tttagcaaaa ctacttttag aaaacccaga tgttctgatc ttagatgaac
    15421 caaccaatca cttagatgat cagcatatcc aatggctgac gcagtttctt caggattttg
    15481 acggtgcgta tatcgtgatt tcccatgacc aagaatttct tgatcagatc accacccata
    15541 tcgcagacat cgagtttgga aagattacta agtatactgg ccatctaaaa caagcattga
    15601 aacaaaagga acaaaatcgc gaaagctatt tgcgccaata tcatgctcaa caaaaacaaa
    15661 tcgaaaagac cgaagcttat attcgaaaat acaaagccgg ttcccgatca acgatggcaa
    15721 aaagccgaca aaaacaatta gataaaatcg aacggctgac gcctccagct tcagcaagta
    15781 aagctgtctt tgattttcca ttctcgccta ttgtcacgac attagcagtg gaaacaactg
    15841 atttagtcat cggatatgat cgaccgctac ttgcacccat caatttgacc atccgctttg
    15901 gtgaaaatat tgccattcga ggcttcaacg ggattggaaa atctacttta ttaaaaacac
    15961 tgatcggtga gattgaaaaa ataagtggcg atttccactt cccagataac acaaaaatca
    16021 attacttttc acaagatctg tactgggaaa atccattaga aacaccacta caatatttaa
    16081 gcagtcaatt tccaaaagcg actatcaaag aattacgtcg ccaactagct aaagcagggt
    16141 tagttaatca attagcaagt gaacctctca ctacattaag tggtggggaa caaactaagg
    16201 tgaaacttgc acagttgaca atgaaccaag gtaatttact gattctcgat gaaccgacga
    16261 accatatcga tcaagaaacg aaagaaagct tacaagacgg gatacagaaa tttccaggaa
    16321 cagtaatcat cgtctctcat gaacaagaat tttatcaaga tcttgtgcaa cgagtcattg
    16381 agattgaacc aaaataaacg tttgattgaa tagttgaaaa aaaggagctg tgacaaaagt
    16441 tttgtcacaa ctccttcttc tgaataaatg gtggccaatt agctgacctt cggtattagc
    16501 tcaaaaaacg gcaaaatatg aggagctatt tttatttggc tattcttatt acttgatgca
    16561 gaacagcttt tcacaacctc ttttcgtcta ttttttattt tcgagatttc tctttccttt
    16621 aaaaacaact gtttgaaaga agaacgcaca tccttgaaaa acaagcccgc taaccgttac
    16681 acctaaccag ccatactgct gccacattgt cgtccctagc aatgaaccag ccgcacctcc
    16741 aataaagtac ataaacataa atacagtatt atttcggttg cttgctgctt cacctagccc
    16801 ttgtactcgt gtttgattgg cgacttgacc aaattgattc ccgacgtcta ataaaataat
    16861 agctaaaagc aaaatggcca aatggcttcc gccaaccaat aaagcaataa agcttattgt
    16921 ttgcataaca agtcccatat acacgatttt tctttcagaa taagaatctg ccagtcgtcc
    16981 cacgataggt gcagcaactg ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa
    17041 tgttccccaa ttgtaggcgg gactgctgat ataaaaaatc aacgtagacc aaaagatcga
    17101 aaatgttcca aacatgaaaa atccactgac tgccgcttct cttaatagac gttgagaagc
    17161 aactaaatgc ggcaaacttt taagtgaatc aaggtatgaa acagctgtac tactttgctg
    17221 gacagaacgc ggcattttcc agtaaagcag aagtgtaaga attccgactg cacataaagc
    17281 aaataaatag atcatccgcc aagaaaaaag actggcaaca ataccagata ccgtccgaga
    17341 aagcaaaatc cccacgagta aaccgctaag caatgtaccc atgaccttcc ctcttgcttt
    17401 tggtcccgct agtaccgcac cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc
    17461 tattaaaaaa gaagcgcatg caaataacgt aaacgaaggc gcaaaaaaag cggccaacag
    17521 agagacggca gataacgctg ccattcgaat gatcaatttt ggacgatcca ccacgtcccc
    17581 taaaggaact aaaaacagta accctaacgc atagcctaat tgggtcaaca tcgtcaatat
    17641 cccgactgca cttgtgctaa cagataaact agctgctact tttgttccaa tcggctggat
    17701 gtaatacata ttggcaacaa caacgccaca agtgattgat aatagtaaaa taagcgaagt
    17761 tgttaaaggt tttttttcat tactcttcat tgatttcccc catttcctat cttagtttac
    17821 aacttatgga aaagattgga aatagaaaaa tacaactatt cgccacactt attatttgtg
    17881 cttttgtttt ccttgttttt tcttgcgatc tttataaatg aagacaacta accctactag
    17941 cacagtaaca cttgcaacca acgtgactgc agttttgtta tatccgtgaa acagagcttc
    18001 tccaccacac gcatacaaca cagacgaagg gatgactcca atcacgatcg acaatatgat
    18061 ttgctgtaga ggcaatttca tcgcatctgc agcaaaattg accatagaag atggaataac
    18121 cggtatcata tagcctaaca ttacacctat ttttggatgt ttcatttgtc ggattgcttg
    18181 cacccaatga ttcgtttcgg ttttcttatc taaaaatttt aagcgatgca taaggaaaat
    18241 tgcagtgatg ttaccaagag cattacccaa aatgttgatg atcgcacctg tcagtggtcc
    18301 aaagctcaaa ccagcaatga ctccaactac tgaagtagga atacctggaa cagcacacat
    18361 caccgctgtc aatacgatca agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc
    18421 gagtagctct gccttactcg ctctcggatt taaaaataat tggatgtctg tctgatattc
    18481 taaatacaac cgatagccca ctaccagtat cagaatgatt cccaacgaga tcaatatcaa
    18541 tcggattatt ttctttttca tatttcctcc tgaaaaaaat ctatattcat taaataatag
    18601 tttaacataa aaacaaaaaa gcaccgaaat ccaacatagg atctcagtgc ttttaaatag
    18661 taaaaattat ttaacagtta cagatgcgcc aacttcttca agtttagctt tcaattcttc
    18721 agcttcttct ttagatacgc cttctttaac tggtgcagga gcgccatcaa ctacagcttt
    18781 agcttctttc aagcctaaac cagttgcttc acgaactgct ttgataactt taactttttg
    18841 gtctccagct gcagttaatt ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc
    18901 accagcagca gctacagcta caggagcagc agcagatacg ccaaattctt cttcaatagc
    18961 tttaactagg tcgttaagtt ctaggatagt tgcttctttt aactcagcaa cgatgttttc
    19021 aatgttcaat gccattataa tttcctccgt ttaattgttc ttttttattt aatgctttat
    19081 gcggcaatta agctgcatct ccatcttctt tgtctgccac tgctttgaca gcgtaagcca
    19141 cgttgcggac tggcgcttgt agtacagata gcaacataga tagaagtcct tcgcgatttg
    19201 gcaattttgc caatgctgtg atttcttcca atgaagagac tttaccttcg atgataccgc
    19261 ctttgatttc taatgctttt gcttctttag caaattcgtc catgattttc gctggtgcga
    19321 ctacatcttc gttactgaat gcaacagctg taggtccagt gaatacttcg tccatgcctt
    19381 caaggccagc ttttttagct gcacgtgaaa gaatagaatt tttgataact ttcatttcta
    19441 cgttagcatc acgaagttgt ttacgtaaac gtgttacttc atcaactgtt aatccgcggt
    19501 agtcaacgac tactactgat gcagctgctg taaatttttc gcttacttca tcaacgattg
    19561 ctgctttttt tgcgataatt gcttcactca tttttgagtt tcacctcctg aattttgatg
    19621 gaagagtttt ttgcactgaa aaggcaagtc acttttatag ttactttgct caaccagtat
    19681 tattcatcac ctattcactt cgttcatagt gattctactc gctttgctcg aaaggaaact
    19741 cctttcgctt tgctcaacca gtattgttca tcatctcttc acttcgttca tagtgattca
    19801 caataaaaaa ctctatgcca ccgcagacat agagggacta ttgaatatat tcaataaacg
    19861 tcctcggtag gaaattaagg cttgatgcca cctactgtct tcggtacaag ttaattatta
    19921 acctcaacta gcttagcatg ctaaactagt tgagtcaaga attatttttc agttggatca
    19981 atcaacattc taatatttaa ttagaaagaa gcttggtcaa catggatacc aggtccaaat
    20041 gttgttgtga tcgttacatt tttcatgtat tgacctttag ctgctgatgg tttagctttt
    20101 agcaatacat catggattgc tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact
    20161 tttccgattg gtacatggat atttccagct ttgtccacac ggtaagttac tttaccagct
    20221 ttcacttcgt ttactgcttt tgttacgtcc attgtaactg taccagtttt agggtttggc
    20281 attaagcctt ttggtcctaa tactcgtcct agacgaccta cagtagccat catatcaggt
    20341 gttgcaacaa ctacatcaaa gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg
    20401 tcacctacga aatctgcacc agctgcttca gcttcttttg ctttgtctcc tttagcaaaa
    20461 actaaaacag tttgtgtttt accagttccg tttggtaata caacagcacc acggatttgt
    20521 tggtctgctt ttttagggtc tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat
    20581 tttgcaatgt ttgtttcttt tgctaaagct actgcttcgg caacaggata aactttactt
    20641 gaatcaactt tttttaatgc atcttgcatt tttttgctct ttttagccat tttggttcct
    20701 ccttgattgt ggttataacg gttgtacctc ccacgtgttc tatatctttc aatatagagc
    20761 caactgagaa gctgctgtct tagggtgtat agattattct acagtgatcc ccatgcttcg
    20821 tgcagttcct tcaaccatgc gcattgctgc ttcaacgtta gctgcgttta ggtcttccat
    20881 ttttaattca gcgatttctc ttacttgatc gctagaaact ttcgcaactt tatttttgtt
    20941 tggttcgcct gaaccttttt cgattttagc tgcttttttc aatagaacag cagctggcgg
    21001 tgtttttgta acgaatgtga atgaacggtc ttcgtataca gaaataacaa ccggaatgat
    21061 caaacctgct tgatcagctg tacgagcgtt gaattctttt gtgaatccca tgatattgat
    21121 acccgcttga cctagtgcag gacctactgg tggagctgga gttgctttac ctgcaggaat
    21181 ttgcaattta acgatttttt ctactttctt tgccacgaga catacctcct taagtccgtg
    21241 atgtggttaa ttggagatgc atatttctcc tcccacgttt tatctgatct gaaaaaatcc
    21301 agacaaataa tgtgcaaaac aatgcacact ggtacagtat agcaaattta tttgtttttt
    21361 caagattttt ttctgctatt tttacatttt gcttgaataa acgctaaatt tattctaaaa
    21421 aaacaacaat ttttcgttct catttcttct tttatgttac acttgcgcta gcatggttca
    21481 acagatgaaa gcaaacaata acacggtttt ttagaccctt gtacaaagat tcctacataa
    21541 caaaatgagg tggagaaaat gaaagattta aggtatagaa gtgtctttga tattattgga
    21601 cctgtgatga ttggtccaag tagctctcac actgccggag ctgctagaat cgggaaaatc
    21661 atacgtagta tatttggcga acagcctgat tctgtagata tttatctata tgaatcattt
    21721 gcaaaaacct atcgggggca cggaacagat attgcattag ttggcggcct gttagggatg
    21781 gaacccgatg atgaacgttt agctgattct ttgaaattgg catatgaagc cggtatggaa
    21841 gtcttgtttg ttcctaagag tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta
    21901 aaaaaaggaa cacgaaagct ttcagtcact ggcatttcga tcggcggtgg caatatccag
    21961 atttctgaat tgaatggatt caaaatttct ttaagcatgg gtactcctac cttgatcgtt
    22021 gtccaccaag atgttccagg tatgattgct aaagtgacta gtatactttc tgagaccaat
    22081 acgaatatcg gcactatgac agtgacacgg gaatcaaaag gtgaaaatgc cattatgata
    22141 attgaagtag atgatcctca agtagaagaa acggtaaaaa gactcaaaca gctgccaaat
    22201 atcgacagcg taaattattt cgattaagga gctaaaaaaa tgtttttatc gattgaagaa
    22261 ttagtagatc aagcaaaagc ttatccaagt gtagcagaat tgatgattgc agttgaaatg
    22321 gaaatgagtg gccgttcccg tagtcacatc atcgagatca tggaaaaaaa cctctcagta
    22381 atggaacaat caatcaacga gggaaaagaa ggtgttacct ccgtcacagg aattaccggt
    22441 ggtgatgcaa aaaaattaga tgattattta aatgaggcag actttttaag tggagaaaca
    22501 atcttaaaag ctgtacgcaa tgctgtagct gttaatgaag tcaatgcaaa aatggggttg
    22561 atttgtgcta caccgactgc cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc
    22621 cgagatcggc ttgatttgac acatgaacag caattggaat tcttatttac cgctggcgct
    22681 ttcgggttag tcatcgcaaa caacgcttct atcagtggtg cagaaggggg atgtcaagca
    22741 gaagttggtt ctgcaagtgc aatggcagca gcagctttgg tttgtgcaaa aaatgggact
    22801 cctcatcaag cagcacaagc agtagccatt acattgaaaa atatgatggg actgatttgc
    22861 gatcccgttg caggcttagt tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct
    22921 caagctttta tctctgctga tatggcatta gcgggtatcg aaagtgtcat tccgccggat
    22981 gaagtcgttg ctgcaatgta ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca
    23041 gcagaaggtg gcttagctgt tacgccaact gctaaacgac ttgcggaaga gattttaaat
    23101 aagagggcgt gataaagctc cttaatttcg agtattaaga gattagttat cttcataaca
    23161 aaaaacgaag agaggactaa ttgcccttct cttcgttttt tattaatcca tcaatttcgt
    23221 attttttact tcatcgatcg tttttgttcc tgcaagttgc atcgtgatcg acaattcttt
    23281 gttcaggtgt tcaaaaacag atgttactcc ttctgcccca cctagattca acccataaat
    23341 cactggtcgg ccaatcgcaa ctaagtcagc accacttgcc agagctttga agacatgctc
    23401 tcctcgacgg acaccagagt cgaaaatcac tggtacacgt ttgttgacta cttcggcaat
    23461 ttttggcaat acttcaaagg acgcaggacc tccatctagt tgacgtccac catgattgga
    23521 tacccagata ccatctgccc ctgcagagat agcgattact gcatcttctg gcgattgtat
    23581 tccttttacg ataactggaa gatgggtgat ttctttgatt gttttgatgt catctggtgt
    23641 caagccttgt tttgcagctg cataaatttc tgcgattcct tttccttcgc cattaccact
    23701 tgcactttgt tcgctatacg cagctaagtt aggcattggt aatggaaatt ggaattgatt
    23761 gatgacgtct tcttcacggt aaccaccgag cgttgaatct gccgttaaaa tgatagcttt
    23821 agcccctgct tctactgctt tgttcaaaat gaactcattg aatctgtcgt ctttactcat
    23881 atataattgg aaaaactgcg gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt
    23941 atttgcataa gtgctgatgg aaaagattga tccagcagca gctactcctt tagctgtatc
    24001 agcttcccct ttttcatgtg ccaatccttg ggccgctgat ggcgcttgga taatcggtgt
    24061 atctaattca attccgaaaa cagatgtatg caaatcggct gagtcgattc cgcgtaatat
    24121 ccgtggcatg attttctttg tattaaaaga agtcgtattt tctttcatcg tccactcatc
    24181 ttcagaacca ccgcgaatat agccaaaagc tcctgcttcc atgcgctctt ttacacgacc
    24241 ttctagttag gcaatgttta caatatcgat tggatgttct tcattacttg tatgataagt
    24301 catagtagat ccttctttca tcttcttgat tacatataaa tcatactaaa cttacaaaaa
    24361 gtaagcaaac atttactctc agatactttt atcgctattc tttccaaata aatcgactta
    24421 caagctgatc tacttcttgg ttttcatgaa gcatactatg agaggcattt tcacctgtca
    24481 ctactttttc ctctatttca tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac
    24541 tgtttaacgg tactgttcca tcactcaagt ctgtttcatc tagttgacca gcaatcaaga
    24601 aaaatcgagt ggttatagga atattaccga tcatttgctg gtaatcttga tagcggttag
    24661 aaacgacagc aggccccttt gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa
    24721 aatcattaaa aggcgcgcca attgctgtta atgtctgaac ttgtggcaat gacgtatcct
    24781 gtccatacgt tcctaaatag cgtagtgcac taactcctcc catggaatgc cccactatgt
    24841 tgactttatc tatgccgtag ttttctttca gatacaaaag acaagttttg atccattctg
    24901 cttgattcca ctcgttattt ttattatctt caaacaatac ttggaccatc ggattgtctg
    24961 cttttccaga caaatctcct gttgcttgta tttgtccatc tgttcctact gttaaaacca
    25021 attcttttac tgcttgctga tttttttcca aacgccgcaa catcccttta aaggaatttt
    25081 ctgtcccgct gtagccgtgg aaaaaaagtg ttggaatagt ggttttttct atcactttac
    25141 tggattctgc cgttttttct ttatcagaaa cttcagtagt atttatcttc gtcgtctggc
    25201 aaccacttaa cgcaaacaaa ctgataagga ctaaagctgc tgcagttgca cgtttgcata
    25261 aatttttgat tggttctgtc atacacctct tcctttccat atagaaaaaa gaaagacttg
    25321 aaaggccaat catatgatcg acatttcaag tctctttatt aatcgatttt atctacttga
    25381 tcgaaatcaa gttctgtact ggtttcacgt ccaaacatat cgatgttgac tttcaatttt
    25441 tctttttctc cgtcaacttc tgttacttgt ccttctaatc cagcaaatgc gccttcgatg
    25501 attttcacga cttcgccaac ttctacttct agatcatttt gtcgcaggct cataccgata
    25561 gaacgcaaga tatgattgac ttcttctgga agtaatggtg ctggtttgct tccggctccg
    25621 tgtgaaccaa cgaatcctgt cacaccaggt gtgttgcgga caacatacca tgaagcgtct
    25681 gtcatgatca tttcaacaag tacgtatcct gggaatgttt tatgaacgat ttctttttct
    25741 ttcccatttt tgacttcagt ttctttttct tcaggaacaa cgacacggaa aataaagtcg
    25801 gccattttca tactttgcgc acgagattcg atatttgctt ttactttgtt ttcatatcct
    25861 gaatatgtat gcaagacata ccagtttttt tcaaaagatt ccattgaagg ctcctcattt
    25921 cattgtaaaa taaaaaaacc tcagtacacc cgaagttttt ctttcgcttc attatagcac
    25981 taaaagtacg ttgtggctag cgagttattt taaaatccag ccaaataagc tttggatggc
    26041 tgtatccatt acgtaaaaca tcaatccgaa aataatagaa gtttcgatga ctactaacgt
    26101 atctttacgg agttgttttc tgcttggcca agttacttgt ttgatttcat cttttacgct
    26161 ttttagaaat ttcatgagac aacctccctt gctactgttt atttcgtttc tttatgtaat
    26221 gtatattgat tacaatattt acaaaactta ttgatttcca aacgttcgcc gctttttcct
    26281 tcactgactg attttgtgta gttgcgagac ccacaaacgg aacaagcgag tgctgctttt
    26341 ttctttgcca tttacttctg cccccttgct gagaatcaga cttatacttc ataacagtat
    26401 cacgtaactt tttacttgtc aatttgtgaa ataaactatc tattgttttg gataaaatta
    26461 tgatatgatt aatttgaaat taagctaaaa tgagacaatt ggaggtatag cgatgctatt
    26521 aaaaacactt gtatataaga aaaaagatct gacaaccgtt acagagacta gtacattaga
    26581 agaagcatta aaaattttag aaacttccgg ataccgttgt gtgccgatct tagattcttc
    26641 aggtaatatt ttccgtggaa acatttacaa aatgcatatc tatcgtcata aagcaaatgg
    26701 cggtgatatg acgcttcctg tcactcatct tttgaaaaac gctacaaagt tcatttacct
    26761 ggatacttct ttcttcaaag tattctttac gatcaaagag cttccttata ttgctgtcct
    26821 tgacagtgaa aatcattttt atggtatttt gacacatagt acgttgctta atatgctctc
    26881 tcaatcttgg agcgtagaac aaggaagcta tgtactgaca attgcttcca caggaaagca
    26941 aggagatttg gctgctattg caaaaatcat ttcaaaacac agcagcattg ccagctgtat
    27001 tactcttgat atcgaaaaag acgaatacat tcgacgtaca ttgatcacgc taccggctaa
    27061 cactgaagcg aaagtatgtg aaacgatcgt caaccactta gagaaaaaga attataaagt
    27121 ggtagaaatt gaaaatttgc aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca
    27181 gctaatctgc tttctttttt cgctttttat aattagacac gcttttcaaa atcaagcgcg
    27241 cgatctaaat aatacatcaa tggtgctgaa acagccatga cgataaactc acttaatgca
    27301 agcgtgccat aggttgtcca aaatgcttgg ccacctgttg gaactaacat ataagcaatc
    27361 agtcccatgc tgatggtaaa gaagatcgtg ttcaatacta aacgcatctt tacatttttc
    27421 actttgtttt gcaataaagc tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa
    27481 accacatcca tgataccaaa accaaagaag gcattataga cgatgacccc gcctaaaact
    27541 ccccaaagca atttacggtt aaagacaact aaatgattca aactttctga aatacgaaac
    27601 tggatcggcc ctgaggatac aggcgcaact aaaatagtta acgctagata aagagccatg
    27661 atgatgccat ttacaacgat tacggataac cgttgtctgc ttttcgtttt ttgcattttc
    27721 tctcctcttt tctcctagtt tttttaacgc gggatggttg atgaaccgcg gttatgtgaa
    27781 acaaacaata attataacag acttttcatt tatttcaatc cgtcttatct gcttttaaaa
    27841 aagcaaaagc ttaaacaatg taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa
    27901 gtgtaagggg tcataagaat tcccagtaaa gcagacgaag taggatctgc aagaacagaa
    27961 ttgttgtgaa acttaaataa ataaaaaagg atgtgggaat ttgagcaacc tcttactaga
    28021 gaacggtcta gcaagcagct gaagatctaa gtggacatgc cgaaccttgg aacttctcct
    28081 tacgtaagta atggatcatg cgcttgctag gcttttttgt gtttttcggt cttcggacct
    28141 ttttcatttt cctagacttc tgctataata accaagaatt aattttgtaa cagatttttt
    28201 tgaaaaaaca agtttctttc attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc
    28261 agcaaacagg agtattaaaa tatcttgttt tattgaatag caggaggaaa tcatgaaatt
    28321 ccattatcat atattgatca atccatcggc aggcagcgga aatggctata aagtagcaga
    28381 aagaatttta ccggtattaa aaaataagca tattgactat acacttcatt atagtgaata
    28441 caaaggacac gatgctgaga ttgctgaaac tttggcaaaa gaaacattga ttccttggat
    28501 agaagaagaa cagaaaacaa tcgatacttt cccccttttg atcatcgtag gaggtgacgg
    28561 caccttacat caggtacttg atactttcta tcagatggaa gtagaattcc cagttgctta
    28621 tattccagca ggttctggag atgattttgc tcgaggggca ggccttccaa aaaatcccaa
    28681 aaaaggactt cagctgattt tagcagctca atcaccagaa aaagtccaca taatggctta
    28741 tgaagaaaaa ataagtgaaa aaaaaggaat agctgttaat aatttcggta taggattaga
    28801 tgcagctatc gtccatgcga caaatcattc ttccacaaaa aagcgattga acaaatacaa
    28861 cttaggatca ttttcgtatt tattctctat tctccgtgct ttattcactc aaaaaggatt
    28921 tccaattctg gtagatgcag gaggaaaaca attaagtttt tcaaacgcat ttttatgtac
    28981 agcaacgaag catccttttt ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc
    29041 taaaccagtc attgattttg tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct
    29101 cattctttta cttatacaga aaaaacaatt gaaatcgaaa tatttccatc actacacaac
    29161 aaacagatta cgtatcgttt caacaacacc gcaacatggt caagaagatg gagaagaaat
    29221 ggaaaaacga ccttttgata tcacaataac cacaactaaa cagttgtttt ggtgttaata
    29281 aaagaggttt tgtcagaagc tacatctgga acaccgttta ttcggaaaaa aggactgtga
    29341 cacgactttt gtcacagtcc tttttttact atgcagttaa ttctttgccc aattgggttg
    29401 ccatcgatag tttctttctt tacccaaaat aaattggatc attgaaaaca ccgcagcaaa
    29461 agcaactaag acacaaccat tgaaccaaaa agattctgca aatgtttgat tgtaactgag
    29521 tgtttcactg atcaagttat tattttgaac aaaccaatat gttggtgtga atgccgctat
    29581 tttgctaacc atttctggca agtattctgc tggtacaaac accccaccga taaagcaact
    29641 gcctgtgata aaaatatctt tgatcccacc gctggtttct gagtttttta ccaatgaagt
    29701 gatacaagcc gaaaagctaa tgatcggcaa taaaaacaag aaagtattta aaatatgata
    29761 ccataccgtt atatcccacg aggtgtggat cactaacaga accattagaa gtaagaagcc
    29821 actgaacaac agcagcgagt aactaatcaa actaaaggtt actttgcgat tcaagtttcg
    29881 tttggtaatg ggcgcactct ggtttcgttt actgatctct ttgcgattaa atgctagatt
    29941 gatgacgctg aaaccgctaa aaatggatag aaacaaccca taagacaaga aattaaaggt
    30001 tgtgcccgtc aacagctttt tcatccgctg actatatccc gcacttagct caacttctcc
    30061 agtaaccgag agtgccattt gtgtctgttc catagctgca tcgattgttt tatcttcttc
    30121 ttgaaacaga atcagtgttt gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga
    30181 aaaggcatct gggcgactct gtatcttgat cgtcggcatc tttcctactt cgacttgttc
    30241 ggtaaagtct gcaggcagat gtaagataat gtttacacga ttaaaataaa gagcatcatc
    30301 gatttctttt tgtgaggttc catccaatgt aacgactgtt tccttgtcat tcaaatattt
    30361 acgaaggcca tcgactacag gcgactcttc ttcactaatc aaggcgattt tcgcatcttg
    30421 cagagaggta ttttcaacat caatattttg cgcaaaaaag aatgtggcaa caacagtgat
    30481 aattacacct atcagtaaat tcgatttatt tgctttaagc aatgtccaaa tcgttttata
    30541 aagcgtcata acgcacccct ctttccatcc aagcatttcc taacgcaaaa aggaaaataa
    30601 atccgatcaa ccatagaaga ttggtataaa aactagccaa atcagtgtag aaataaagtt
    30661 t
//
LOCUS       DAZULO010000023        29407 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000023 DAZULO010000000
VERSION     DAZULO010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.024"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..715
                     /locus_tag="ACG8OA_001064"
     CDS             59..715
                     /locus_tag="ACG8OA_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4975892.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(1157..1954)
                     /locus_tag="ACG8OA_001065"
     CDS             complement(1157..1954)
                     /locus_tag="ACG8OA_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFS4975893.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1951..3135)
                     /locus_tag="ACG8OA_001066"
     CDS             complement(1951..3135)
                     /locus_tag="ACG8OA_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFS4975894.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(3160..4407)
                     /locus_tag="ACG8OA_001067"
     CDS             complement(3160..4407)
                     /locus_tag="ACG8OA_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS4975895.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            4622..6367
                     /gene="dnaX"
                     /locus_tag="ACG8OA_001068"
     CDS             4622..6367
                     /gene="dnaX"
                     /locus_tag="ACG8OA_001068"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFS4975896.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            6387..6701
                     /locus_tag="ACG8OA_001069"
     CDS             6387..6701
                     /locus_tag="ACG8OA_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFS4975897.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            6726..7322
                     /gene="recR"
                     /locus_tag="ACG8OA_001070"
     CDS             6726..7322
                     /gene="recR"
                     /locus_tag="ACG8OA_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFS4975898.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            7386..8030
                     /gene="tmk"
                     /locus_tag="ACG8OA_001071"
     CDS             7386..8030
                     /gene="tmk"
                     /locus_tag="ACG8OA_001071"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFS4975899.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            8045..8374
                     /locus_tag="ACG8OA_001072"
     CDS             8045..8374
                     /locus_tag="ACG8OA_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFS4975900.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            8388..9326
                     /gene="holB"
                     /locus_tag="ACG8OA_001073"
     CDS             8388..9326
                     /gene="holB"
                     /locus_tag="ACG8OA_001073"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFS4975901.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            9362..10186
                     /locus_tag="ACG8OA_001074"
     CDS             9362..10186
                     /locus_tag="ACG8OA_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFS4975902.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            10179..10526
                     /locus_tag="ACG8OA_001075"
     CDS             10179..10526
                     /locus_tag="ACG8OA_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFS4975903.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            10595..11467
                     /gene="rsmI"
                     /locus_tag="ACG8OA_001076"
     CDS             10595..11467
                     /gene="rsmI"
                     /locus_tag="ACG8OA_001076"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFS4975904.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            11576..12697
                     /gene="dinB"
                     /locus_tag="ACG8OA_001077"
     CDS             11576..12697
                     /gene="dinB"
                     /locus_tag="ACG8OA_001077"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFS4975905.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(12751..13353)
                     /gene="nrdG"
                     /locus_tag="ACG8OA_001078"
     CDS             complement(12751..13353)
                     /gene="nrdG"
                     /locus_tag="ACG8OA_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFS4975906.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(13668..15857)
                     /gene="nrdD"
                     /locus_tag="ACG8OA_001079"
     CDS             complement(13668..15857)
                     /gene="nrdD"
                     /locus_tag="ACG8OA_001079"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFS4975907.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            16183..16950
                     /locus_tag="ACG8OA_001080"
     CDS             16183..16950
                     /locus_tag="ACG8OA_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4975908.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16940..18982
                     /locus_tag="ACG8OA_001081"
     CDS             16940..18982
                     /locus_tag="ACG8OA_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4975909.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            19263..19412
                     /locus_tag="ACG8OA_001082"
     CDS             19263..19412
                     /locus_tag="ACG8OA_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFS4975910.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            19423..20937
                     /gene="dltA"
                     /locus_tag="ACG8OA_001083"
     CDS             19423..20937
                     /gene="dltA"
                     /locus_tag="ACG8OA_001083"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFS4975911.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            20934..22139
                     /gene="dltB"
                     /locus_tag="ACG8OA_001084"
     CDS             20934..22139
                     /gene="dltB"
                     /locus_tag="ACG8OA_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFS4975912.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            22184..22417
                     /gene="dltC"
                     /locus_tag="ACG8OA_001085"
     CDS             22184..22417
                     /gene="dltC"
                     /locus_tag="ACG8OA_001085"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFS4975913.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            22420..23688
                     /gene="dltD"
                     /locus_tag="ACG8OA_001086"
     CDS             22420..23688
                     /gene="dltD"
                     /locus_tag="ACG8OA_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFS4975914.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            23829..24911
                     /locus_tag="ACG8OA_001087"
     CDS             23829..24911
                     /locus_tag="ACG8OA_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFS4975915.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            24911..25714
                     /locus_tag="ACG8OA_001088"
     CDS             24911..25714
                     /locus_tag="ACG8OA_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS4975916.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            25784..26524
                     /locus_tag="ACG8OA_001089"
     CDS             25784..26524
                     /locus_tag="ACG8OA_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4975917.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            26535..27539
                     /locus_tag="ACG8OA_001090"
     CDS             26535..27539
                     /locus_tag="ACG8OA_001090"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFS4975918.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(27584..>29407)
                     /locus_tag="ACG8OA_001091"
     CDS             complement(27584..>29407)
                     /locus_tag="ACG8OA_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975919.1"
                     /translation="YNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSPADSNQMK
                     EIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQW
                     ITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKV
                     YLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFA
                     LALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCT
                     FQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTP
                     WIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQV
                     DFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILE
                     EAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIH
                     ALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQE
                     RFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         9293 a         5178 c         6053 g         8883 t
ORIGIN      
        1 aaaagtttta aagtactgta tgatcttttt agatgaaagt ttggatgggg gagcatcatt
       61 ggatagtaga gtcttaaagg aatatttgga taaaaatgat tttccgatta tacgcaaagg
      121 atatcataaa tatttgacat tcgaagggat ggaagattcc catacgtata tcttgaaaga
      181 gggaacggta aaagcaagtg ttatttcaaa tgatgggaga gaatttaacc ttcgctatat
      241 caaagatctt gaaatcgtct ctcttttaaa agacgaatat tcacaattca tcgattcgcc
      301 gtataatatt cgagttgaat cagaatatgc agagttttat cgaatcaatc gtgtgcgctt
      361 ttggcaaaat atcaaccaag acccaaagct acaacactat gtaaaagagt attaccgcta
      421 tcgtctgctt tattcgatga aaaaaatgca gcaaatgcta ttaaacggaa agctgggagc
      481 tgtttgtacg caactttacg aattatatga tttattcggt gtgcggacag aagaagggat
      541 gctgatcgat ttttcgatca cgaatgaaga aatcgccaaa ttttgtggca tctcaactgc
      601 tagcagtgta aacagaattt taaaacaact gaaaaaagat ggtattatca ccgttaaaga
      661 ccatcatatc gttattttga atatggactt gttagaagac aatattatct tttgatgaac
      721 aaagaggttg tgaaatcagc gttttgacct gagtattaga gaaacaaacg gaaaaatagt
      781 tcctcatatt ttgccgtttt ttgagctaat accgagggtc agctgattga acaccgttta
      841 ccaagaggtt gtgagcctgc cgtttaactc tgagtcacaa ggaacaattt caaaaaacag
      901 cttttcatgt ttgatgaata ttgtgacttg tgaccgagga gttggtagga gaacaccgtt
      961 taccaagagg ttgtgaaaaa gctgttctgc atcaagtaat aagaagagcc aagtaaaaac
     1021 agtctcttat gtttttaact tggctggact tatcaccgta gatgtaagct tttgaacgcc
     1081 gtttatttgc aacaaaaggg gctgtgacac gatttttgtc acagcccttt ttttgcgaac
     1141 attcatttag caatttttag aaacttctgc cgccaccgcc aaaagtcccg ccaccgctag
     1201 aatgggtagt acttccgcca ccgccgcttc caggaggtgg gcttttgggg attcttcttg
     1261 tcgtgacaaa agaatttgtc agacgatcgg ttttttcagt taatttgata ctggattttt
     1321 ctcggaatgg atatttgtag atgcctgatt tcaattgata acgcgaaaca gtcacaaaat
     1381 aaaagacaag actaaggata agagccaaaa caacagcaat gatgatttct actgtcgtca
     1441 aaaccttata gcgtgtaatt ttgccagttt cttcgtctac acgataatgt cctcctggaa
     1501 ctccgtcttc cacatatgtt gctgatttag acaggtaagc ttttgcagca ttgtaatagg
     1561 cagcatttgt catctgatca taaacatcat ctaagatcga atcgattctg ctatcagtca
     1621 aataatcgat catattacca gaagtcgaaa tgtagatttc acgctgtccc atatctaata
     1681 gcaatactga gccattttga tcatttccga ctgcgtctct taagtaatta tcagcaaagt
     1741 ctcttggttc ttctgaatta gatgtagtcg ttacgatcaa gactcgtcct ttgatttttt
     1801 cattcagctt ttctgcctgt tgttccaaag attggatttg atctgttgtg aataacccag
     1861 cctcatcatt taccgtagct gtatctgcat gagtagccac cggccagcca acacttacta
     1921 gcaaaagaaa gaataaaaaa aactttttca tcatatcaag taacctccga tcagaaagag
     1981 gatcgctagc ccagcgaaaa tcccaagagc ggtcagtccc aattttttct ggctgatcgg
     2041 caggacaccg ctgacctttc ctgtttgtcc attcattgcg tagtagtaga cttttttact
     2101 tgagtcattg cttcgataag taacaagcca taccggcagt aatacgtaat tatttacttc
     2161 gtttgtgatt gaagcagaag ttcgcacacc ggtcaatgtt gtatatccat ttgcggtatc
     2221 ttttaacagc ttctctgaat agtcttttaa ttcagattga atctgtgatt tgattgcctc
     2281 atattcgata tctctttttt ctgcttggaa cccggctaaa tactggcttt taaaatcaat
     2341 tgctttatct aacgggaaag gttgcacgcc ttcgaccatc ttttgttgcg tattttttga
     2401 taaggcattt tttactagtt ctttaaaaga aagcttgcct gctcttgaaa cagcaaattg
     2461 cttcgtttct gtgtattcga tgtcacctat ccgccatatg cggataactg taccattcgc
     2521 ctgcatggca ccatctactt cagcatctgt agcccaataa gggaaataca cacctgtcaa
     2581 cttatcgatt tgatccttat tgaaaaaagc tttgggaaca aaccattttt tcttggtcca
     2641 agcgagaaat ctgttgattg cttcttcttt ctcgacagca aaaggaagta ctttatttgg
     2701 caagaattct ccgcttaaac gtcccgatag tacgacggga ttatggcagt agtaacaata
     2761 agtcgcagca gttgtcgcat cagtcacgat ctctgcacca cagttgggac aaagaaaaag
     2821 atctatcgct gcggtattat ctgcagattc gtccgctgta tctgaaaaac cgcctgcctc
     2881 gtcaaccgct ttgcgctcgg cttcattcat atcggcaagc tgttcttggg ctgtaaaagt
     2941 aaaatcttcg gtaggtgaag gttcttcttt gctttcttcg ccagccatac gcgcttcttt
     3001 ttgcttttgc tcatattggc tgacttcttc ttctgtatag atattcaggc agtattcgca
     3061 atgaaatttt tggtctttgg gatcaaaagt caacggaccg ccgcaattag gacatttatg
     3121 tgtaagaaca tccatcagct gcacaccttt ctaaataaat taattaacgg ggattccttt
     3181 aaacggttta ccacagtttg gacagaattt agggatgccg ttggaaagat ccacgatctc
     3241 attgcattca ctgcatcgca tttgtagctt aggctgaacg ttttctacag gtttggcagc
     3301 tccgcagttg gaacagaatt ttccggaatt ttctgtcccg caaactggac atgtccaagt
     3361 atccgcattt ccttgtgcag tttgttgatt ttgtttttct gcttgttgtt tgatttgttc
     3421 ttggttattt tgagctgctt gagaaagata agaaccattt gcattcattc ccatacctac
     3481 acccataaag ccggtcattg ctcctgcttc attttttccg gctgcttcca taccacgtgc
     3541 aatggaacct tgcacgtatc cttcacgtac gctaggatca cctagcatcg ccccttcgtt
     3601 acgcatattg atcagtttca cagaatcatc ggtgtaagaa atgctggcta cagccacaga
     3661 aacaatctcc atcccacgta attcacgcca agaatcatcc aatgcagtat ccatgtattt
     3721 gcttaattcc aaactcttag atggtacata agaaatccgt tgaccatctg cagacatctg
     3781 attgattgct gattgtagcg ctgttaggaa ttcagctaaa tactgctcat tgatatcatt
     3841 gatttccact tgtgttttgt ttttagggat tgcgtttgta taaaataaaa tcgggtcaac
     3901 gacatgaata gaataagtgc catgtgcccg taagaataat tctgcgttat agaaattgtc
     3961 aaagtagttc aacggcgcgc ttgtaccgaa tttgattccc tttatttctt ggagattgat
     4021 ataaaaaact tgttgtttct gcggtgtgac gccgccaaat ttgaatcggt caaaagtttc
     4081 agcaattgct tctttcagtg ttccattgaa catggaagga gctgcgtcat ttttaatggt
     4141 gtaataacct tcctcagcgg tataatcgat gatttttcca ccatcgacca aaagcatcat
     4201 catgtttgga tagacatgga cgactgttcc gtctgttaaa acatcttctg ttcctttacg
     4261 attggagcca cgtttgtcat ctttgcggac tttgacacct tttgtcatga ccgttgtatc
     4321 actcatattg tctggttcga tgacttctag ccattgatct gccagaccgc cgccaaccat
     4381 acttgttgct gctttgataa gacccataaa aaattcctcc tctgattcag ctagtgccaa
     4441 acaattattg tttatttatt ataccatagg aagatgtgaa acttctcgga ctagagacgg
     4501 aattgaaatg gaaaattgcg tattgtttaa atacctgaaa aagccgagaa ggatagtagt
     4561 ttttttacct aatatcacgt ataataagag atagtgaatt ttttaaaaaa gggggatcct
     4621 tatggcatat caagcacttt atcgagtctg gcgttcgcag cgtttcgaag acatcgttgg
     4681 acaaaaagct gtgacacaga cactaaaaaa tgctattgtt tcccacaaaa cctcacatgc
     4741 ctacttgttt acaggaccca gaggaaccgg aaagacgagt gcagcaaaga ttttcgcaaa
     4801 agcaatcaac tgtccgaaca gtaaagacgg tgagccatgc aacgaatgcg aaatgtgtcg
     4861 atcaattact gctggtacgc aagaagatgt gatcgaaatc gatgctgcca gcaataacgg
     4921 agtcgaagaa atccgattta tccgtgatcg ggcaaattat gcgcctacaa aggcagcgta
     4981 caaagtgtac attattgatg aggtacatat gctttcaaca ggagctttca atgccttgtt
     5041 gaaaacattg gaagagccaa aagaaagtgt catattcatc ttagcaacga ccgaacctca
     5101 caaaatacca gctacgatca tttcacggac tcagcgattt gattttaaac ggatcaatac
     5161 ggcagatatt gtcgaacatc ttgaatttat cttgaaagaa tcgactatca gctatgaacc
     5221 agctgcattg aatgtgattg cccgttctgc agaaggcggg atgcgggatg cattgagtat
     5281 tcttgatcag gcaatctcgt ttagtgaagg aacaattact ttagatgatg ccatgcaagt
     5341 gacaggtagc ttaactgacg aaatgatgga cagttacttg agtgcttgcg tgaaaaaaga
     5401 agtaccagaa gcattggaaa cattgaacca aattctcgca gccggcaaag aatcacgtcg
     5461 ctttctagag gatatgttac aatactgccg tgacttgctg atgtatcagc aagcacctaa
     5521 cctagttgag gaacaatcga gtacgatcag aagtacattt acagaattgg cacaaactgt
     5581 ttcagcggat cagctgtttt caatgataaa gattttgagc gatactcaaa atgaaattcg
     5641 ttttaccaat agtgccgcta tttatttgga agtcgctaca gtcaaacttg cacagctacc
     5701 gtctgcttct gttccttcag cagatgtagc atttgcagat gatcagatag taaaacagct
     5761 acaacaagag attaaggaac taagaaacga aatcaaggaa ctacgcagta atacaggaat
     5821 tgagaataca aaagtacagc cggtacagca aacaaatcat aaaaaacaga caagtaccta
     5881 tcgtgtaccg acggaacgag tatataaagt tctgcgggaa gcgacaagac agcatctgaa
     5941 ccaagtaaaa gaagtatggg aagacttgct gatgagtcta tctgtcactc agcgagcaat
     6001 gcttaaagca agcgaaccag ttgcagctgg acctacagga ctagtgattg cgtttgatta
     6061 tgagattgtt tgtcaaagag cagcaaatga cgaagaattg cagctttcgg tacacaacaa
     6121 cttaagccga atgatcaaag attatgcgcc tgatcctgtt tatattacaa gagaaagctg
     6181 gccagaactt cgaagaaatt atttgacgca ggcacaagaa gagggaacag aaaactcttt
     6241 tgcttctgaa gactcagaag gagaaatcga gttgcttcct caagaacaag aagtaccaga
     6301 agaaacactt gtagtaaaag cagaggaact gtttggctcc gaagctgtta cagtggtaga
     6361 tgattaataa aaaggagtag ataattatga tgcgtggaat gggcaatatg caaggcatga
     6421 tgaaacaagt acaaaaaatg caaaaagaaa tggcagaagc acaagagctg ctaaataaaa
     6481 cagagtttac aggtaaagcc accaatgaat tggtcaaagc ggtttttaca ggcgatcgtc
     6541 gcatgaaaga tatccaaatc aatgaagaag tcgttgatcc tgatgatatc gagatgcttc
     6601 aggatcttgt cgtaatggca gtcaatgatg cgatcgcaca aattgatgtg gaaactgaga
     6661 aaaaattagg gaaatacgca aaaggtttgc caggatttta gttttaaact gaaggagtaa
     6721 tgaatatgca atatccagaa ccaattgcga aactgatcga cagctacatg aaacttccag
     6781 gaatcggtca gaaaacagcg acaagactcg ccttttatac gatcgatatg aaagatgaag
     6841 tagtaaatga atttgctaaa tctttgttaa gtgtaaaacg cgatttgcat ttttgtagta
     6901 tctgcggaaa catcacagaa gaagatccct gcgaaatatg tagaaatcct tcacgtgatc
     6961 gaagcgttat cctagtggtc gaagagccga aagatgtaat ggcaatggaa aaaatgcgag
     7021 aatatcacgg actttatcat gttctccatg gtgtgttatc accgatggaa gggacaggtc
     7081 cagaagacat caatatttct tctctgttgc agcggctgca tgatgcagat gtaaaagaag
     7141 tgattatcgc aacaaatgca acaacagagg gagaagcaac agctatgtat ctctcacgtc
     7201 tgatcaaacc cgcaggtatc aaagtgactc gtctagcaca tggtctgtct gtaggctctg
     7261 atatcgagta tgcagatgaa gttactttat tgaaagctgt cgaaggaaga cgtgagttat
     7321 aaatttctaa ttaagtgttt tttcagatac aatataaaag cagcacaaga gatgggggca
     7381 acaatgtgaa cggactattt attacaatcg aggggcctga tggtgcagga aaaaccagca
     7441 tcttgaatga actttaccct cttttgaaag aagtagcaaa aacagaaatc attcaaacaa
     7501 gagaaccagg aggcattccg attgctgaag agatccgagc agtgatcctt gatccaaaaa
     7561 atgatcggat ggatgaacgt acggaagcac tgctttatgc agcagcaaga agacaacatc
     7621 tagtcgaaaa ggttctgcct gctctggcaa aagggaaaat cgttttatgc gatagatttg
     7681 tcgacagttc tcttgcttat caaggtgcag gccgacgcat cggcgttacc gagattgctc
     7741 ggttgaatga gtttgctaca gaaggtacta cgccggattt taccctttat cttgacgtag
     7801 attcagacac cggattaaga agaatcaaga aaaacagaca aaatcaaatc gaccgtttag
     7861 attcggaagg tttggaattc caccagcgtg tacgtcatgc ttacttaaag ctggcagaag
     7921 agaatccgga aagaatccac aaagttgatg caagaaaaag ctttgaagaa gtgctgcaaa
     7981 caagttatca tacaattatt gaacagtatc cgcaattttt tgaaaactag gaaggtgaac
     8041 gtttatgaaa attattttag cgattatcca agacaaagac agcaaccgtt tatcaaatga
     8101 attgattgat gcaaatatcc gtgccacaaa actatcatcg acaggcggct tccttaaggc
     8161 tggaaacagt acgtttatcg tgggtattga agatgaccgt gtagaagaag cattggaaat
     8221 catcaaaaaa acgtgtgaat caagaaagca atttgtatct acacctgtta cactggatat
     8281 ttcgatggat gggggcgtcc cttatccagt agaagtagaa gtaggcggtg ccacagtctt
     8341 cgtcttacct gttgaaggat tccatcaatt ttaggaggag ctgagctatg aatcaagaaa
     8401 taaacttggc tcaaatgcaa ccgatcgtgt atcagcagct tcaacggagt tttgagcatg
     8461 ggcgtatcgc tcatgcttat ctttttgaag gggagaaagg aacaggaaaa catgaagtag
     8521 gtatatggct tgcacagcac ctgttctgta cccaaatgaa agacaatctg ccttgtggaa
     8581 aatgcaacaa ttgtcagcgg atcgaaaaga aagaacatcc agatgttttg gttattgaac
     8641 cggaaggaca aaccatcaaa gtcgatcaga tcagacgttt gcagacggaa ttcagtcgca
     8701 gcggttacga atcaagaaaa aaagtttttt tgatcaaaga agctgaaaaa atgaatagca
     8761 gtgcggcaaa cagtttgttg aaattcttgg aagagccccc tggagatttt ttagcggtgt
     8821 tagaaacaga tgcagtagga agaattctcc ctactattca atctcgttgt caagttctgc
     8881 attttcaaga actttcgaaa gaagcactga tccatcgatt gcaagaagag cagattccat
     8941 tggaaaaagc aaaacttttg gctttcttaa caaacagttt aggaaaagca gttgaaatct
     9001 ctcaagatga atggtttaat gatgctaagg acttgattta tcaatggttc gtttacttgc
     9061 aaaagcaaga tactcaagcc tttatctatg tccaaaaaaa attggtaaaa tcatttaaag
     9121 aaaaaacaca acagtttaca ggattatctg tcttgatgtt ttattatcag gaagcacagc
     9181 gtcaagcagt agcaaacggc gaatatggca aagtgaaaca gataaaccag ataattgaac
     9241 ggatattgat cgcagagcag aaactacgca gcaatgtcag ttttcaggct gttgcggaac
     9301 agttcgtttt gcatacgatt ttttagcttg ccgcaaagga tgatgatcaa ggaggatgga
     9361 aatggtagaa gtagtaggtg tccgatatag agatgcaggt catatctatt attttgcacc
     9421 aggagactct gaatatgttt atagtgacaa ggtattagtc gaatctcagc aatccttaca
     9481 tattgctact gttgtccttc ctaaaaaaga gattgatccc aatgaccttc ctgaagctat
     9541 ccgtccgatc ttgcataaag cgtcacaagc tgaattagaa aaagacaaaa aaaacaaaaa
     9601 agatgcttat gatgcattga aaatcgcaaa agaaaaagtg cagttgcgtg agttggaaat
     9661 gaaaatggtc aaagccgaat acacatttga tcgaaataaa ttgatttttt ctttcacagc
     9721 ggacgggcga attgacttta gagaattggt aaaagatctg gccgctgtat ttcgtactcg
     9781 tattgagctt cgacaaattg gtgtccgcga tgaagcaaag ataataggcg gaattggtcc
     9841 atgtgggcgt ccattatgct gttcttcttt tttaggcgat ttcgtgcctg tatcaatcaa
     9901 aatggcaaaa gatcaaagtt tatctttgaa tccaacaaaa atttccggtt tatgcggacg
     9961 cttgatgtgc tgtctgcaat atgagagcga agaatacgag acagcgaaaa aagaactccc
    10021 tgactttgga aaagtaattg ctacacctga tggtaaaggg aaagtcgtag ggttgaatct
    10081 gcttagccgt atcgttagta ttcggctgac tggacgggaa aatcctgtcg aatatgaatg
    10141 ggatgaattg aaagaattta tccaagtagg tgagatgaat ggataagaga tcattatacg
    10201 acggattgaa tcaattagaa tcagagttga gaaatacatt aacagaactt gttgaaatga
    10261 agcatgcttt gcatgagatc gtagaaaaaa atacaacttt ggagatggaa aatcaacgtc
    10321 tgcgggaaca cctgcgggaa ttgaaccaag ttgcacaagc tccagctgaa aatgtgaagc
    10381 aggaactttc taagtctcgt atgaatcttg aaaaaattta tgaagaagga ttccatgtct
    10441 gctacgattt atatggatca agaagggaca atgacgagcc ttgtgcgttt tgtctagaag
    10501 tgatctatcg cgaaagcggt cgatgaccaa taacaagagg agactggagc ggagaaatac
    10561 tgctccagtc tttttattaa ggagattgtg acagatgtat aatcaaaaaa gtttcaaggg
    10621 aaatccggta tatggggatt tatatcttgt accgacgcca attggaaatt tagaagatat
    10681 gagctacaga agtgttcgta tgctgcaaga atccgatctt atcgcaagtg aggatacgcg
    10741 taatacacaa aagttattga atcattttga aatccataca ccccaaaaaa gtttacatga
    10801 acataattat aaagaacgtg tccctcaatt gattgctttg ttaaaagaag gaaaaacgat
    10861 tgctcaagtc agtgacgcag gaatgccttc catcagcgat cctggacatg agctagtttt
    10921 agcctgcata aaagaaggga tttctgtgat ctctatccca ggacctacag ctgggatgac
    10981 ggctttgatt gcttcagggc tagtgcctca accattttta ttttatggct tccttccacg
    11041 taagaaaaaa gaacagaaag aaacattgaa aaagctaaaa gaacagacag ttacgctgat
    11101 tttttatgag tcaccttatc ggatcactgc tacattgacg aattttttag aaatttttgg
    11161 aaatcgccaa gtcgttttgt gtcgggaatt gacaaaggtc catgaagaat accttagagg
    11221 aagtacggaa gagctactcg cttatatcga agaacatcca gtaaagggtg aatgttgtct
    11281 gcttgtagaa gggaatttgt tcgaaatgcc agtgacggaa gttgatgaag agttgggaac
    11341 gttaaaggaa caagtagaag caaagatcgc ttcaggcgaa aaaccaaatg ctgctatcaa
    11401 agcagtagcg ctgaaaaatg ggctgaaaaa acaagaagtc taccgtcagt accatgaatt
    11461 agactgagat agtataagtt gtctataatg aaagactaat gaggaaatca gctactaact
    11521 tttcaaatct cttgaacgcg aacgaatgtt cttgtataat gaataagagg tgaggatgat
    11581 ggagctgcaa tttcctttac ataatgatac atcaagaaaa atcattcata ttgatatgga
    11641 tgcttttttc gcttcggttg aagaacgaga tcatcctgag tttcgcggac atccattagt
    11701 aattgccaga caccccagtg acactggtgg gcgtggagta gtgactacag caaattatga
    11761 agcaagaaaa ttcggaattc attcagcaat gagtgcacaa aaagcgtatg agctttgtcc
    11821 gcaagccatc tttaagcctg gcgaccatca taaatacagc gatatttcca aacaagtacg
    11881 ggaagtattc tatcgttata cagatattgt ggaaccagtt agtatcgatg aagcgtatct
    11941 tgatgtgacc gaaaataaaa tcaattgcaa gtcagccatc aaaatcgccc gaatgatcca
    12001 agcagatatt tgggagagtg tccagctaac atgttctgcg ggagtaagtt acaataagtt
    12061 tttggccaaa ttagcatcag actatcaaaa acctagagga atcacggtag ttgcccctca
    12121 agacgctgtg ccatttttaa aagctttgcc aattgaaaaa tttcatgggg tagggaagaa
    12181 aactgtacct agaatgcatg agctagggat ttatacagga aaagacctat atgaatgcac
    12241 agaaatgatg ttgattcgaa attttggaaa aatgggctat tctctttatc gtaaagtcag
    12301 aggaatccat gattctccag taaacgttac aagagagcga aaatctgtgg gaaaagagca
    12361 tacttatggg cagccgttgc aaacggaaga agcagtgctg actcagttgc gccaactagc
    12421 tgaaaaagta gaagaagcac tgagaagagt ccaaaaacat gggaaaacag tagttttgaa
    12481 ggtcaggtac acggactatt ctactgtgac caaaagagtg acattgccag aatatatcta
    12541 taaaaaagaa gcactctttt atcaagcaag ccttatatgg gaggaaatct tgggagtaga
    12601 aaaaggaatc cgattgttag ggattacttt gacgaatctg gacccgatga cttatgaaaa
    12661 catcgtcctt ccattatggg aaaatcaaga aatataaaaa cagctttgag agttgaaacc
    12721 ggaaatccgt tttctctccc aaagctgttt ttatatcaag tcttgctttt tgatttgttc
    12781 ataggtttct gttgcatccg tacatttttc ccaaatcaca acttgattgg attctaaaga
    12841 tttttggacg tcaatgatcc gctggttgct acttccgcga aattgtagtt ttaggtcacg
    12901 tttgcttaat tcaaagcgtc cgtcaacaag gatatcgatt tgcgaaagga gttctaattt
    12961 atcttctgaa tcttgtagca attcatcaaa ggcatagcct gtccatgacc agatatcttt
    13021 tgtcgtaccg aactcttttc ggatacgtcg tacgacttgc agacaaacat ctgtattcaa
    13081 aaaaggttcg ccaccaagta aagttaatcc ttgcacgtaa tcgttgcgca agtcagcgat
    13141 gatttgttct tctaattcag gtgtgaatgg tttgccatat cggaaatttt gtgcagcttt
    13201 gttgaagcag ccttcgcagg caaataagca gccactaacg taaagactgt tgcggacacc
    13261 ttcaccatcg acaaaattaa acggtttgta gtcagcaata tagttttggc tgtagtcttt
    13321 tgctaaccat tcttttggtt ttggatttct cataaaaaaa cttctttcat ttaaggttca
    13381 aagatatagc tattgaagat aaaatctact cgaccacata aataagaaca aactgaccta
    13441 aatccattgt tcaagaaagc ttgttcttat tttgatagag ggcgttcaaa gatacaaagc
    13501 catcgaaaga gggtggtgaa aaaagacttg tcatagcctc ttattctgaa taaacggtgt
    13561 tcaaaagctt gcatctgcag taataagccc aactaaacga aaaatatgag gaactatttt
    13621 tgtttagcta ttcttattac ttgatgcagg acagcttttt cacaacctca tttcatgtgt
    13681 ttcacacgtg aggaaatttc tttatggcgg ccatgtacca tcggacgagc ttgcggattt
    13741 cctaaatagc cacaagtacg tttgactaca tcacatgttt ttggatcatg atttccacat
    13801 tctggacatt cgaaccctcg ttctgtcggt ttgaaatcac cttcgaaacc acattgatag
    13861 caatgatcga ttggcgtatt cgtaccaaga tacccgattt tgtcatatgc aaaatcccat
    13921 acagcttcta aagctttcgg attttgttgg agcatagggt attcacagta gtggatgaat
    13981 ccacctgaac aatattttgg ataatccttc tcaaattcca atttttcaaa tggcgttggg
    14041 tttttacgca catcatagtg gaaactgttt gtataatatt ctttatctgt gatattttcg
    14101 atgatgccga atttttctgt atccaaacgg cagaaacggt cagttagact ttcacttgga
    14161 gttgaataga cactgaaatg ataaccatat tcatcccccc atgcatctgc attagctttt
    14221 aattctttga cgatatcaag tgtgaaagct tttgcttctg ggtttgtttc ccattcgcca
    14281 ccgaagaaag ctgaagcaac ttcatacaat ccaatgtagc ctaacgatac agtagctcga
    14341 cggtttttga ataattcatt gacagaatcg gtacgtgaaa gacgtttccc gaaagaacca
    14401 tacatatata aaattggcgc atttgctgga atggcttctt tacaacgttc tacacgatag
    14461 agaagcgcat ctttcatgat ttctaagcgg tcagacagta actgccagaa tttattctga
    14521 tctccttttg attcgatcgc gattcttggc aaattcaatg taacaacacc taaattcatt
    14581 cgtccgacat ttacttcttg accattttcg tctttccacc cttgcaagaa tgaacgacag
    14641 cccataggta ctttgaaact tccagttaat tcaacgattt tatcgtaatt cagaatatca
    14701 ggatacattc ttttcgttgc acattccaat gctagttgtt tgatgtcgta attaggatct
    14761 tcagggttta agttcacacc ttttttcaaa gtgaagatca gtttagggaa gatagctgtc
    14821 cgcttttcac tccctaatcc attgattcga atctgtaaaa ttgctttttg aatttctctt
    14881 tcaaaccagt tttctcctag tccaaatcct aatgaagtga aaggagtttg tccatttgaa
    14941 gtaaataagg tattgatttc atattccaaa ctttgcatag catcgaagat atcttttttg
    15001 gtttttgctt ttgcatattc tttttggcgt tctggcgaat cgatccattc ttccgcgtct
    15061 tttagatgtt ttttatagtt caattctgca aaaggagcaa gcaattcatc tgttcggtcg
    15121 gcagaacagc caccatattg acttgacgct acattggcaa taatctgtga gatttgtgct
    15181 gtggcggtct ggatagattt tggcgattcg acttcggcat tgccgatttt gaacccattg
    15241 ttcagcatgc ctttgaaatc aatcaagcag caattcgtca tcggtgtata aggatgataa
    15301 tccaaatcat ggtaatggat atcgcctttt tgatgcgcat ttgctatatg tggtggcaac
    15361 attttcagtc cgattgattt tccaacgatc ccggctgtca gatctcgctg tgtattgaaa
    15421 acattactgt ctttatttgc gttttcgttc acgacagttt ggtccttgtt gattagcttt
    15481 tcaatcgaaa agttgatatc tgttgcttta ctgcgagcaa aatctcgttt ggtacgataa
    15541 ttgatgtact cttctgctaa ttcatattcg tttttttcta gtaatatatg ttccacgatg
    15601 ttttgaatct catagatttt gacattttca gcaaaccggc ttttgatttc ttggacaacc
    15661 aagtctgcaa tcatttgaat tttttcataa gtaagtggtg ataatgagcc gtgaattttc
    15721 ttttctgctt tgatcagtgc ttcattgatc ttttgttcat cgaaatcaac atggcggcca
    15781 tcgcgtttga tgactttcat ctgggatgtc tctggctgct gagtaacgac agttttttct
    15841 tcataattta ctttcattat cttcaactcc tgctaaattg ttcttgtcta tcataaaaca
    15901 aaatcgggga aggagcaact aaatatcggc tatatatagt attcaatgtg ctgtataatt
    15961 ggatttctat actatatgta gtgtatattt tattgtgaaa aaaggtgaaa acttgtagct
    16021 ggtggatttg ttttttgcat atttaatttt gctgaataaa ataattgaat aaaatttttt
    16081 attcagtcca agtaaaagtg acaaaaacat aagagcaaat tattcgtaga tatgctacta
    16141 tgcaaataaa tagaaatgaa aaatgaaaga ggaggagaag aattggaaaa gatgatcgaa
    16201 gtgaaacatc taagtaaaag ctacggcagc aaactttctc ccgtcaaagc cttagataat
    16261 atctcattca cagtagaaga aggcgagttt gttgggatta tgggcccaag tggtgcagga
    16321 aagacaacgc tgatgaatat cttgtccacg atcaatttgc ctacgatggg cagtgtctcg
    16381 atccaaggaa aagaaatcac aaaaatgaaa aacagtgagc tcagtgactt tcgtcgaaaa
    16441 aaactaggat tcatttttca agagttcaat ttgatcgata ccctaaatgc aaaagacaat
    16501 atcctattgc ccctagcagt tgaacggatg actaaagagg aaatggaaaa gagagtaaga
    16561 catgtagctc aattgttgaa tatagaagaa ctgctcaaac ggtatcccga tgaactttct
    16621 gtcggacaga gacaacgaat tgcagctgct agagcacttg ttgttcagcc acaagtgatt
    16681 tttgcagatg aaccaactgg ctctttggac tcgaagtcgg ctacagaatt gttgaactac
    16741 ttgaagacca tgaatcaaaa agaaaaagca acgatactgt tagtcaccca tgatccatat
    16801 acagcaagct actgcgatag aattttattt attaaagatg gtgtgatttt ttctgaagtt
    16861 gtcagaagag ggacaagaag agagttcttt gagaaagtaa tagacatgca ggcgacgatt
    16921 ggcggaggag gaaaaatgaa tgctgtataa actagccgtt aaaagttttt ttcggcagtc
    16981 acgaggctat cttgtttatt ttttcagttt gactttatcc gtaatgatct attattcctt
    17041 cagcgcaatg acctacgatc aatctttagt cagaagagca agtcaagata cttcgataga
    17101 tggtggattg agttttggtg gatttatcat tactgttgtt ttgctttttt tcgtattcag
    17161 tgccaaccga tttttcttga atagaaggca aaaggaaata ggcatctatc agctttttgg
    17221 tatgaataaa ctgcagatat caggtatata tgtgttagaa atcatgataa ttggattgtt
    17281 cgcctgtatt tctgggatat tactcggtat tattttttcc aaactctttt caatgatttt
    17341 ggttcgaatg atggatatgg atcttactag tccatttttt atctccattc catcagtagt
    17401 agatacttta tttgcttttt tcatcatcct tttagcagta tctgtatatt caatatggaa
    17461 aatatggcgc tatccaatga ttcgaagttt tggagaaaaa gaacaagcag atagttcaac
    17521 aatgcgcttc aggacaaggc accgattact cgcagtcatc gggttactac ttttaacgag
    17581 cggatatttt ggagctagtc attttaggga aatcacttca tggatgatca gttatcatta
    17641 tcttgataca ttagttttct tttttccttt tttaattttt ttcgtttgtg tggtcggtac
    17701 ttatttgttt ttctgttata catttcggtt atttttgatc atattgagta aatggaagat
    17761 ttattactat ggtatcaatt ttttgataat gggtaacacg caagttcatt tgctcaaaag
    17821 ttggcgtaca aattctctga ttacagtcat tctcggaatt tctttaatga caattggcgg
    17881 cacggctagt atctctacta ttttatccta ccaggagaaa gtttcctccc cgatggacta
    17941 tcagtttgat gttgagacag agaaaaaaat caagccgatt ctagcagagg aagggcagaa
    18001 gataactaaa gaaatcacct tgcattataa agtcattggg agtatctata gtcaaaatct
    18061 cgaagaacct atgtttcaaa ctgctaatct gatcacggaa acacaatatc aagccttcag
    18121 aaaagttaat ccagatttgc cgaacatttc tttgaaatca cctggtcata ctgttttgct
    18181 tgatcaaatg caaactcttt ttcggaatat ttcattatat ggatctagta ttggactgcc
    18241 agataatcaa gaattgaaga ttcaacaaat gcttccaagt gtattaggtg acgaaacaat
    18301 ggtatatcta agtcctgtac tgattgtaga tgataccgtt tttgaaaaag caaaaggtgt
    18361 ggattatcaa gtagtcaatg tagatgtatc aggtggaaat aatgaaaaaa tagatgaacg
    18421 actgagcaat gacctgtcga taaagtggct caatgctgtt tattattctt atgatatcgt
    18481 aaatggaagt ctacaaggag aaattagcac aaagaaactt ccggatgacc aaatgaagga
    18541 agcacaattt agccaagaaa gctctcgttt gaatttctct tccagctatt cgaaattgcg
    18601 tgcagttaat cggcgaatcg gtatcaatat ttttgtcaca ctatttgtcg ggatgatcgt
    18661 gattattgct actggtagta ttttgacagt ccggcaatta gcagaagcag aggaagaaaa
    18721 agaaaattat cacctattga ctaagctggg gattccacaa agaaggatca gacggatgat
    18781 cttcgagcaa aacgcactta ccttctttcc acccatgata ttagggatca ctcacgcagt
    18841 atttgcaatc aatgtcttca ctcagtatat aaaaactgcc gattactggt tggcttatct
    18901 tgtgagtggc ttattgatcc ttatctatct tttgctttat gtcattacga gtcagttata
    18961 ttgtcggatt atcgaggaat gatatacgga ataagacaag ataataaata gaactaaatt
    19021 ttgaatacca tttatttgga ataagaggtt gtgacacgat ttttgtcaca acctctttat
    19081 tttttatatt ttttaaaagt acgagaaatt ataaagaaaa aagcagtgat ttgatgtgct
    19141 atttttataa aagctatgtg acaaaactgt tagtaaaaaa acggtttctg taagctagac
    19201 acatgtttct ttttgtcaaa attagttaca ctttgtgtga ggttatatga ggagggaatc
    19261 caatgaacaa atggcgttct aatccaaaat tgaattattg gggaaaattc atcattcgaa
    19321 caatttttta tagtgcagta ctgctgttcc tgatttatct gtatcactat aaaaatattc
    19381 aaggaggaac ttttatctat aacgaatttt aggaggaaga gtatggaaat caaaacgatt
    19441 attgaagcaa tcgatgattg gggaataaat gaacctgatc gagttgctta tcaggcggat
    19501 gagacgcata cttttggtga attgaaacaa gcatcagacg cacttgctta ttatttagaa
    19561 aaaaaggtag aaggcgatgg accaattgtc gtttttggca atttggagtt tgaaatgatt
    19621 gtctcatttc taggggttgt caaatctggc catgcctatt tgccgattga agagcataca
    19681 cccaaggagc gtatcctatc gatctttcgg gtggctaaac catctatggt catcagtatc
    19741 ggggactgga tagaagatat tccttcggtt cctgtcataa caaaaaaaga atttcaagaa
    19801 atcaagaaga taccagtagg attttatccc agaaatagcg taaaaggtac agaaaatttt
    19861 tatattattt ttacttctgg tactactgga gaaccaaaag gcgtacaaat cagtcatgat
    19921 aatctagtca gctttgttca atggacgatg gaagactttg ggatcaagcc tggtatgcat
    19981 ttcttagccc aagcgccatt ttcttttgat ttatcggtat tttctattta tcctgcatta
    20041 gtttctggcg gaatgctgaa gccgttaaat aaagcagttg ttcaagactt tcgtcaatta
    20101 ttcgcgacat tgccagcctt gaagttgaat gtgtgggttt ctactccatc atttatggat
    20161 atttgcttga tggaacctac attcaatgga gaaaatgtgc cggaattgga catgttttta
    20221 ttttgcggag aagaattgac gaaaaaaact gcagaaagtc tacttgatcg attcccaaat
    20281 gcacggatat ataatactta cggtccaaca gaagcaactg tagctatttc aagtattcag
    20341 attgaccagc atgtactaga tagttatgac cgactaccga ttggctacgt aaaaaaagac
    20401 acgaaagttt ctattgtcca agaaggtaaa cctgtttcaa aaggagaaac gggtgaaatc
    20461 attattgcag gaccaagcgt atcaaaaggt tacctgaaca atcccgaaaa gacagctgct
    20521 gcgttctttt gtcaaaatga cttagcaagt taccatacag gggatgcagg tagactagat
    20581 gaatatgggc ttttatttta tgaaggacgt atggatcagc aggtcaaatt acatggatac
    20641 cggattgaac tcggggatat cgaacattat ctgctccaag acaaccgaat caaacaagct
    20701 gtcgtcgtac caaaatacca aggaacaaaa gtacagcagc tggttgcttt tgtcgtatta
    20761 gaaaacaaaa cgaaacagcc ggattttcag ttgactaaat ctatcaaaga acaattacta
    20821 gaagttgtaa tggattatat gattcctcag aagttccatt atgtcgatct gctgcctcag
    20881 acagttaatg gaaaaatcga tcgaaaaaaa ttaacagctg aggtgaatcc tgaatgatgt
    20941 tacctcatat gatcccttat gcagagccgt tttatttttt tcttttagcc attgcgcttg
    21001 taccgatcgt tctatcttta ttgatatggg aaaaaagatt accagtttat caatcagcag
    21061 tgacactttt ctttttattt gtcagctttg gaggtaatta ttggcaacag ggagcggcgc
    21121 tgattgggta tgttttgtgg cagacgatac ttgtatgggg ctattttgtt tatcggcaaa
    21181 ggaaaaatca gacaggatgg ttttttgccg ccgttatttt agcgataatt ccactgtttt
    21241 taacgaaagt gactcctttt tttgaacatg gaaaagcatc gttacttggt tttttaggaa
    21301 tctcttattt aacctttaaa tctgtacaaa tgatcatgga aataagagat ggcatgatca
    21361 aaacatataa gccatttcgt tatatccagt ttctgctgtt ctttccaaca atctcttctg
    21421 gaccaatcga tcgctatagg agatttgaaa aagatcagct ccatccgcct gttcgtgata
    21481 aatatgttga tcttttggga aaaggcgtcc aaaatatttt tgtcggtttt ttatataaat
    21541 tcattatcgg ttattatttt ggtcaagtac tgcttcctgt agtgggacgc attgccttac
    21601 agcatggtgg tatttcatgg gcattagtag gttatatgta cgtgtacagt atgtatttgt
    21661 tttttgattt tgctggctat agcttatttg cagtcggtac aagctacatg atgggatatc
    21721 agacaccaat caatttcaat aaaccttttt tatcatggaa tatcaaagaa ttctggaatc
    21781 gttggcatat gacgctgtcc ttttggttcc gtgattatgt atatatgcga ttgatgttct
    21841 tcatgatgaa gaaaaaattt tttaaaagca agatcgtaat gtcaaatatc ggttattttg
    21901 ctttgtttct tctaatgggt atatggcatg gattgacgtg gtactacatc gtatatggac
    21961 tgtatcatgc ttgtctgatc tgtctgacag atgcatggat ccgatttaag aagaagcata
    22021 aagagcagtt gccttctaac aaatttacgc atggcttagc cgtatttttg acatttcaag
    22081 cagtatgttt tagcttcctg attttttcag gatttttaga taaacttttt tttagctaac
    22141 gaatagtgac atattaattt atataacaaa ggagaaaagg aaaatggaag aacaaatttt
    22201 atcaatctta gaagaaataa caggaacaga tgaagtaaga aaaaatcgtg atattgatct
    22261 atttgatgaa gggttgcttg attctttagc atctgttcaa ttattagtcg agctagacgg
    22321 agagctggat atccaagtcc ctgtatctga attcgagcga gaggattgga gcacaccgaa
    22381 taaaatcatt caacaagcaa ctgcattgaa gggttgagta tgaaaaagaa actttttgct
    22441 attttcggcc cggttgtttt agctgttggc ttactgtttg tgttttttac ctcctcctat
    22501 caagtagata tctctaatcc agaaacgatt agacgtgcct cttcttccat gtcagtgaac
    22561 gtgttgaaag gagatgcgat aaaaaattat gcattatctg aaaaacaata tattcctttt
    22621 tttggctcaa gtgaattaag cagaatcagt ccatttcatc cgtctgttct ggcagaaaaa
    22681 taccagcgaa actatcgtcc atttttgctg ggagcacccg ggacacaatc gttaagccaa
    22741 tatatgatga tgcgttctgc aggagatgct atgaaaaaca agaaagtcgt ctttattatt
    22801 tctcctcagt ggttcgttaa aaatggtgta aaaactgatt actttaatac gtattattct
    22861 gaacttcaaa catacgactg gttgttttca atgaagaaag taacgcctgc tgatcgctat
    22921 ttagctagaa gattattaac tttctcaaaa gtaaaagaga atgatacgtt gacggcgatt
    22981 ctgcaaacga tcaaaaaagg gaagctacct ttacctgaat ctttgaatca gctgcgcagc
    23041 caatggaaca tgctgaagag agaggatgaa gtattcagca atattgggtt gaaagatcga
    23101 cagcaaaaaa tcgatcatga gtcaaaacga ttaccaaaac aataccaaga aacagaatta
    23161 tccattttag caaatcaaat tggagaacga gaaacgacga ataatccatt cgggttgaaa
    23221 aacgattttt atacgcatcg tatccgagcc cacgagccag agctcaaaca gtcacaaaaa
    23281 aattgggatt atcgtttttc gccagaattt tctgattttc agctggtctt ggatcaatta
    23341 gcaaaaaacc ataacgaagt attgttcatc atcccaccgg ttaacgagaa atggagcgat
    23401 tataccggac tatctcaaga aatgttacaa ggatttgcca aaaagatcaa atttcaatta
    23461 aatagccaag gattcaatcg aatagctgat tttgtcaatc aagcgggtac caactatttt
    23521 atggaagaca cgatccatct aggctggaaa ggctggttgg ctgcagatca acagatcaga
    23581 ccgtttttag aagaaaatca tattactgct agtaagtatc atcttgacga tgcttttttt
    23641 agcaaatctt ggcaacatca aataccagat aaactacaat taaaataaag aaaacaggcg
    23701 agtgttgctc gcttgttttc tttatttttg aatattctga ataaaagata aaaataccaa
    23761 gtttgcctga acaaaaatca taagcaatgg tataatcaca atatgaatat agaaatgggg
    23821 gaaaacattt ggatacaaaa gaagaacagc tacttattga tttaacttct gcaaaaggag
    23881 tacctggaaa tgaagaagag gtaagagaag tattcagaga atatgccaaa ccattcgctg
    23941 atgatatttt ttatgacggt ttaggcagtg tgattgccaa acatggtgct ggcggaggcc
    24001 ccaaggtctt tatttctgga catatggacg aagttggttt tatggtaaca aaaataaccg
    24061 aaaaaggatt cttggaattc caaactcttg gaggttggtg ggggcaagtc atgcttgctc
    24121 agcaagttga aatcaaaaca cgtgaaggga aaatcgtcca cggtgtgatt ggttccaaac
    24181 caccacatgt tttaacacct caagtaagaa ataaacctta tgaaataaag gatatgttca
    24241 ttgatattgg cgcatcaagc caagaagaag ccaaagagtg gggcatacgt cctggagata
    24301 tggtcactcc atatattgaa tacaaacgaa tgaatggcag taaatattta ctggcaaaag
    24361 catgggataa tcgtatcggg acagctgttt ctctgcgagt attagagaat ctatctaaag
    24421 aagcacaccc aaatgtttta ttcgcaggaa gcgatgtgca agaagaagta ggcttgcgag
    24481 gtgctcgaac aagtacacac cttgtaaatc ccgatattgc ttttgcttta gatactggta
    24541 cagcaggaga cactccagga atgacaccaa aagaagcaga ttcgatttta ggaaaaggcc
    24601 cacagatttt gatttttgat gcatctatga tcccgcataa aaaattattg aattttgtta
    24661 ttgatgtagc agaggaaatg gatattccat tccaatatac agtgatttct ggcggaggaa
    24721 ctgatgcagg acaaatgcat ttgacaagag atggtgttcc gtctgtagcc attaccgttc
    24781 ctgttcgtta tctgcattcc catacatcag tgattcatga agatgattat ttgaatacag
    24841 tgaaactagt cacagaatta gtgaaacggc ttgatagtga aacagttgaa aaaattcgga
    24901 gttatgaata atgaataata gaatacctat ttctgaatat cggaaaaaag cacgagtttc
    24961 tttggaaaat caatggggaa tcaatacttg gcttgttttc ttatctataa tatttacgag
    25021 cgtcatccag aacttttttg acggtttcag tccttttccg gaagaaagca tgcagcagca
    25081 cttattgagt tttctagtcg ataatttact tttattcgca ttcaactatg cattgtttta
    25141 cattgcgcta gtagttgtac ggggtggcag accaaaaagt aaccttttat tttcagtttt
    25201 ccaaagaaag tattttggtt cgatacttgg catcaatttg atcaattcag tcatcaactg
    25261 gctactcaac agtttaatct ttctgcccat gtttttaatc agcggagtga gtacttatat
    25321 gcagctgctt ttcagttcaa atgcggtacc tacgggtgtc atgccagaag attttcttga
    25381 tatcagcttt gttttatcga tgatagggat gatttttgtt tcagcactca tcatgagtat
    25441 tgttggcggt ttattccaat ttgctgcatg gacaaagttc gactatcctg atttgtcggt
    25501 tattcaatgt ttgaaatatg gctggttttt attgaaagat agaattggta cgtatatttt
    25561 gttgcagctg tcttttatcg gctggtattt gctaggagca atcgctttgt tcatcggtct
    25621 tttgtgggtc attgtttatg tgaatgtgtc agttgcagaa ttttatgaac aagcacggat
    25681 tgaaaaagga ggtccagacg cctattttac ttagcatgta agcagtttat tcatcaaaaa
    25741 aataactttt gtggtaggat aaatctttga gggggaatgt acgatgaaat taactgtttt
    25801 aggatgctta ggagcgtatc cttataaaaa tcaagggaca acaggttatt tgcttcaatc
    25861 tgacgggttc aatttgttga tcgatgcagg tagtgctacg ctgatcaaat tacaagaaca
    25921 ccttgatcca cttgatctag atgctgtgat ccttagccat tatcatcatg atcatattgc
    25981 tgaccttggt gtattacagt attactggca acttcatcct aagagaaatc cggagacatt
    26041 gctttcaatt tatggacaca caaaagattc cattcacttt gaagaattga cgttggaaaa
    26101 tgtctcaaaa ggacatcctt attttgaagc tgaagaatta aaaattggtc cttttctgat
    26161 tacttttatg gaaacaatcc atccagtagt atgttacgcc atgcgtatcg tagaagaaac
    26221 aacggggaaa gtattcgtat ttacaggtga ttcaggttat ttggaaagtt tcgctgattt
    26281 tgcgaaagat gctgatttgt ttctagcaga tacctatctt tttgaaggaa atgaacatca
    26341 tcatgcccat ttcacttcaa aagaagctgg agaacttgcg aaatcagcta acgtaaagaa
    26401 acttgtactt acacacctac ctcagtttgg tgatttagaa caattgaaaa atgaagcgca
    26461 aaaggcagca ggcaaaggga ttgatataga acttgctgca gttgacaaag tgtttaacat
    26521 ttaggaggaa gaacatgatt tttgtaccaa atgaaaacca cgacccaaga atcaatttag
    26581 caattgaaca atatttgctg caagaaatgc ctttagatga acctattttg ctcttttata
    26641 tcaatgaacc ttcaatcatc atcggcagaa accaaaatac gcttgaagaa atcaatcgag
    26701 attatgtgga agaaaatggc attcatgttg tccgtcgctt aagtggaggc ggagctgtct
    26761 accatgattt tggaaacctg aactttagtt ttatcatgcc tgatgatggg gattcattcc
    26821 gtgactttgc gaaagtaaca cagccaatca tccaagcact tcatgaattg ggtgttgctg
    26881 gggcggaatt gaaaggccga aatgatctag tcattgatgg catgaaattt tctggcaacg
    26941 cgatgtatgc taccaacggt cggatgtttg ctcatggaac aattatgttc gactctgata
    27001 tcaatgaagt agtcaatgca ttaaaagtaa aaaaagacaa aatcgaatca aaaggaatca
    27061 aatcgatccg ttctcgtgta acgaatatca aaccatttct tccagaagaa aagcaaaaaa
    27121 tgacaacaga agaatttcgt caagaaatct tgttgaaaat tttcggcgta gagtcagtag
    27181 aagaagtaaa aacgtacgaa ttgactgatg aagattggaa aaaaatcaat cgaatctctg
    27241 aggaatatta tcgaaattgg gattggaatt acgggaagtc tccagatttc aatttctctc
    27301 gccaaaaacg tttttctatc ggttcgattg aagtccattt gaatgtctca gaaggaatga
    27361 tcaaagaagc aaaaatcttt ggcgatttct tcggactagg agatatctct gacgtggaaa
    27421 aaaaattggt tggacagaaa tatgataaag catctctatc aaaaatagtc agccaaattg
    27481 atgtgaagaa atattttggt gcgatagaac cagaagaatt gcttacctta ctttattgaa
    27541 aataaaaaat agcggaatcc acatatatgg attccgctat tttttataca ggattcgggt
    27601 aatttcgaga gagttgaaca gtgatccgat caatgaaagt atctttttcc tgcgttagtg
    27661 ttcctgtgta acgatccgct acgtctttca tcatcattaa gtcaaaacgt tcctgatcat
    27721 ctaattcttc tgtgatgcct cgactaaata ggtttgtatc gtaatgtgca ctatgttcgc
    27781 aacaaatgac gaaatgttcc tcttgttcgt gtatcgacaa ataaattttt cgatacagtg
    27841 gattttcgat ggcatgagtt tctctaaagg aatgctctaa agcgtggatc aaaagttgag
    27901 ccagatcgtc atcgttgata ggaagttctt cgggaagatc tgcttggaag gttatctgaa
    27961 tattccgttt ggcagctaac ttctggtaac gcgctaagat aagatttgtc acctggtggt
    28021 tgcaaaaaga acacagcatt tcgaaatttt ttgcttcttc taatatcttt tccacatagt
    28081 ttttagcttt tgtttggtcc gtatgcatta aatcttgcag attctccatc tcttggacaa
    28141 attccaggcg aagtgtattt acttcttgga tatgctgtcg aagctgctca taatgttgct
    28201 ccaacaaatc atttttgatt tttaagaaat ctacttgtct ccgatctttg tcggtattta
    28261 agatgtgatc gattagattg tagctgataa taacgattag aatgataata tataaaatcg
    28321 tagaccaatt gatcgttgtt tgatagatac ctaatgccgt ttggatgtaa atcaagcaat
    28381 ggataatggc taccaagacg atccaaggtg tacaagctac ataaaaacgg ttattcttat
    28441 atgcttcgcc aatgcatgca agtgaagtga ctaaagtgct tataacacta gcaatattag
    28501 caatcggcat taattcagga agttctgcag gagtaaaagt tgcataaagc agaacgccga
    28561 taaaaatcaa aagttggaac gtacaaaaaa ttccgtacca ttttttatag taaaacattc
    28621 ggctccaata gtagcagatg atgaaagctg acgttaagat aataaataaa tgagataaaa
    28681 aagaatgaac gaatggttca aaaagaatac cacttaaaat atcttctgat acagcttcaa
    28741 atcctaatgc caaagcaaag caacttaaga gaataagtga gtattcaaga cgtttttgtt
    28801 tatacatcaa gtagatcgta tagaaaaagg tccctatagc gataaaaaca gcgattatca
    28861 tcgtcaatac ttgcggcact gattgtgaat aaataaaact aatcaatggt ccagttgttc
    28921 ctagataaac tttcggtggc agtccagcat agttctgata aggagatgag actgtaaggg
    28981 tcaatttttt tccgatataa tttgctggta aatctatcac agccaatgag agtccgggat
    29041 tgctttcttc ctgataatct ttccgttggt ataaaatctt ttcgcctaga tatacagtaa
    29101 tccattgatg gtttcctttg attagtaaat ttgcttcgtc caacggtttt tcaagtgtcg
    29161 tttccataat gaatgtttcg tctttatcca cggtaggaat cgtacgagtg tacttgctgt
    29221 caaacttaaa atcaggatca tcttttgtat agaaaaccca cgaatctttt atttctttca
    29281 tttgattgct gtccgcagga gaaaaaataa aataaaagaa ataagcaatt cctgttaaaa
    29341 ataacaatga taatataatg attatgctcc actttatacg taaataaaaa gattcttttt
    29401 tattata
//
LOCUS       DAZULO010000024        28281 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000024 DAZULO010000000
VERSION     DAZULO010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.025"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1626
                     /locus_tag="ACG8OA_001092"
     CDS             <1..1626
                     /locus_tag="ACG8OA_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975920.1"
                     /translation="KNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIG
                     LDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLT
                     MVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCY
                     EIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLK
                     ATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLF
                     LFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNG
                     FSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAP
                     EDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSN
                     NKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIA
                     SRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1771..2511)
                     /gene="truA"
                     /locus_tag="ACG8OA_001093"
     CDS             complement(1771..2511)
                     /gene="truA"
                     /locus_tag="ACG8OA_001093"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS4975921.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2702..3337)
                     /locus_tag="ACG8OA_001094"
     CDS             complement(2702..3337)
                     /locus_tag="ACG8OA_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFS4975922.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(3484..4704)
                     /gene="sstT"
                     /locus_tag="ACG8OA_001095"
     CDS             complement(3484..4704)
                     /gene="sstT"
                     /locus_tag="ACG8OA_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFS4975923.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            4923..5921
                     /locus_tag="ACG8OA_001096"
     CDS             4923..5921
                     /locus_tag="ACG8OA_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975924.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            6117..7952
                     /locus_tag="ACG8OA_001097"
     CDS             6117..7952
                     /locus_tag="ACG8OA_001097"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4975925.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8012..8728)
                     /locus_tag="ACG8OA_001098"
     CDS             complement(8012..8728)
                     /locus_tag="ACG8OA_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFS4975926.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8955..9497)
                     /locus_tag="ACG8OA_001099"
     CDS             complement(8955..9497)
                     /locus_tag="ACG8OA_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFS4975927.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9502..9729)
                     /locus_tag="ACG8OA_001100"
     CDS             complement(9502..9729)
                     /locus_tag="ACG8OA_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFS4975928.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(9808..11688)
                     /locus_tag="ACG8OA_001101"
     CDS             complement(9808..11688)
                     /locus_tag="ACG8OA_001101"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4975929.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11877..12491)
                     /locus_tag="ACG8OA_001102"
     CDS             complement(11877..12491)
                     /locus_tag="ACG8OA_001102"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4975930.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            12633..13193
                     /locus_tag="ACG8OA_001103"
     CDS             12633..13193
                     /locus_tag="ACG8OA_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4975931.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13498..13812)
                     /locus_tag="ACG8OA_001104"
                     /pseudo
     CDS             complement(13498..13812)
                     /locus_tag="ACG8OA_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(14066..15701)
                     /locus_tag="ACG8OA_001105"
                     /pseudo
     CDS             complement(14066..15701)
                     /locus_tag="ACG8OA_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16256..16819)
                     /locus_tag="ACG8OA_001106"
     CDS             complement(16256..16819)
                     /locus_tag="ACG8OA_001106"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS4975932.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(16834..17877)
                     /locus_tag="ACG8OA_001107"
     CDS             complement(16834..17877)
                     /locus_tag="ACG8OA_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975933.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(18010..18414)
                     /locus_tag="ACG8OA_001108"
     CDS             complement(18010..18414)
                     /locus_tag="ACG8OA_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS4975934.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(18647..19282)
                     /locus_tag="ACG8OA_001109"
     CDS             complement(18647..19282)
                     /locus_tag="ACG8OA_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFS4975935.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(19548..20600)
                     /locus_tag="ACG8OA_001110"
     CDS             complement(19548..20600)
                     /locus_tag="ACG8OA_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4975936.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(20613..21551)
                     /locus_tag="ACG8OA_001111"
     CDS             complement(20613..21551)
                     /locus_tag="ACG8OA_001111"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS4975937.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(22002..22652)
                     /locus_tag="ACG8OA_001112"
     CDS             complement(22002..22652)
                     /locus_tag="ACG8OA_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975938.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(22752..23261)
                     /locus_tag="ACG8OA_001113"
     CDS             complement(22752..23261)
                     /locus_tag="ACG8OA_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFS4975939.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(23462..24331)
                     /gene="scrK"
                     /locus_tag="ACG8OA_001114"
     CDS             complement(23462..24331)
                     /gene="scrK"
                     /locus_tag="ACG8OA_001114"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS4975940.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(24351..25472)
                     /locus_tag="ACG8OA_001115"
     CDS             complement(24351..25472)
                     /locus_tag="ACG8OA_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFS4975941.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(25474..25869)
                     /locus_tag="ACG8OA_001116"
     CDS             complement(25474..25869)
                     /locus_tag="ACG8OA_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4975942.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(25888..26685)
                     /locus_tag="ACG8OA_001117"
     CDS             complement(25888..26685)
                     /locus_tag="ACG8OA_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4975943.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(26687..27475)
                     /locus_tag="ACG8OA_001118"
     CDS             complement(26687..27475)
                     /locus_tag="ACG8OA_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4975944.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(27489..27965)
                     /locus_tag="ACG8OA_001119"
     CDS             complement(27489..27965)
                     /locus_tag="ACG8OA_001119"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4975945.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(28037..>28281)
                     /locus_tag="ACG8OA_001120"
     CDS             complement(28037..>28281)
                     /locus_tag="ACG8OA_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4975946.1"
                     /translation="LGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQ
                     KFQTHFKQSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         8891 a         5688 c         4358 g         9344 t
ORIGIN      
        1 aaaaaccaaa taatagatat catttttatg ttatttctaa gttttgctta cgtcatccct
       61 ctattcatgt ctaagggaat ctatcatagt gtcaatcagg atacttactt tcatttatct
      121 cgaatcatag gactagataa tgtatggagt tctccagtaa acttcaataa cttcgatcat
      181 catggaacaa tgatgaatat tttttatcca tggcttactc tttatccagc ttttttattt
      241 tataaaatga tgggaaatct tgttttaagt tataatatct actatttttt tatcactttt
      301 ttaacaatgg tcgtttctta tttttctatg aaacaaataa aaaacaatcg ttatatttcg
      361 ctgttatttt cgattattta tacattttcc gcctatcgtg ccattgacat attccatcgt
      421 gcttcattgg gagaagctgt cgctttaaca tttttgcctt tgatactaat ggggtgttac
      481 gaaatttata ttagagatta ccaaaaatgg tactggttgt ctattggcat gacgctagta
      541 gtgtatactc atttactttc tgtcgctatg gtttctgtat ttattggagg aacgttgttt
      601 ctttcttttt atttttggga ccaaaaaatc gctagactct taagtctttt aaaagctacc
      661 gtcctcacct tttttttaag tgcgggattc ttaattcctt ttattcaaca atctagagca
      721 caagaattga aagtcccact gggaaaagaa ttaagtggta tggctcctag tgatatgctg
      781 acacatattt taaataataa ctataacaac tatactattg gattattctt atttctcgga
      841 ttgattggtg cttttatttt tataaaaaaa ttaactgctg atgatttatt tattttcttt
      901 cttggtgtat ttgttctttt ttgttcaacc aatttatttc cttggcaact ttttaaccat
      961 acaccagtta agagtctaca atttgtctgg cggttaaatg gtttttcttc tttatttata
     1021 gcctatacga tgagtatagt gatctattat attttttcta ctaaaaataa ttcatggaaa
     1081 ataatggtct ttacgtttct tgcactaata ttgcatctct caggagtaag caattctatc
     1141 catgctaaca aagattcatt aacacttata gcacctgaag acgctgttgc tatagctgaa
     1201 aattacaatc atactgatta tgcaaacaaa gagtcaatct atcatcctga tatgattaat
     1261 aatgatcaat atttactagg taaccaggaa ataaatccaa caactcattt tatgagtaac
     1321 aaacttacaa ttgaattaga taattcgaat aataaaaata ctgtcttgac gacacctatt
     1381 tatcgttata aagggcaagt ggcttctatt aatggcaaac tagtacaaac aaaattatct
     1441 aagtttggca caactgaatt aactattcct ccaggtataa ataaagtcgt tattacttat
     1501 cagtacacaa aactagcaat tgcttcaaga tatttatcta tcgttacttt gattttattt
     1561 ttactttatc gatttacttt ttctaaacaa aaacaaccga gaagagaagt acaacattct
     1621 cactagattt ttatttaaga aagacttttt agctgtacac ataaacaggc taaaaagtct
     1681 ttttttatat ttctttaatt tgcttcatac attatttaac atcgatattt atactgtgaa
     1741 acatgcgttt catccattat caaagtattt ttatcgataa taaacttcat ccaaaaacag
     1801 accttgtgca gggacggttt ctcctgcatg ttgccgcact ttgctttcaa atatttcatc
     1861 gattgtttct atcggcatgg cacctgtgcc gatttctagt aatgtcccca tgataatccg
     1921 caccatttta tataagaatc cgtctccgac aaaagtaaaa tgaagcattt ctccctttcg
     1981 ttcgatagat aattcatcaa tcgtacgcac tgtggatttt tttgattttt tcaatgcaga
     2041 aaaaccaatg aaatcatgtg tacccactaa tttttcgcaa gctgcattca gcttgtccat
     2101 atccaactct tgagggaaat aaaagctgtg gtttcgctca aatgctgaag gaatcacgtt
     2161 gttccaaaca taatagctgt attttttgcc caccgcatta tatcttgaat ggaagcgctc
     2221 aggcatttct tcgattcttt tgatgacaat atcccgcgga aggtaacgat tcataaagtc
     2281 gatcatttct gccagtccaa tttctgtgtt tgttttaaaa tttgctactt gtcctcgcgc
     2341 atgggtgcca gcatctgttc ttcctgatcc aataatttct atctcttcat ttgtcatctg
     2401 atgcaatact ttttcgattt ttccttgaat ggttttatcg tcatctccaa gtctttgcca
     2461 tcctgaatat ctttttccgt catattcgat tgtcaacttg atgtttctca tattttagtg
     2521 gctcctctgt cttccatttg gtcttctctc taccttttta agattgtagc acaaaatata
     2581 gctccttgct tctctaagac agaagctata caagaaaact gtggaaatat atatttcaga
     2641 aaaacaaaaa acagccaccg gatgaagtat ccgctgactg ttcaatttga cggctcaagt
     2701 tttactgttt gagatgcaag atactcaacc tggcaatggg cacataatta aagaaaaaac
     2761 tgattctttg gcaaaaacct attttattca gtaatggcca agaatttata gaaggaattc
     2821 ttgccagtcc ataaatcgcc gcaaaaagca aactgatcac agacagtcca aggtataagt
     2881 ggcttacgtc catttgccca ttttttcgat agatcaagaa aagaagaact ggaaacaata
     2941 aaaaaccact gtaacctaaa cctgatccga tattatggac ccatgtcgag acattccacg
     3001 aagattcatg ggtattgata ctgaacaaac ctgtaaagat acaatcacct ataccataga
     3061 acgcgatggc tgctgtaact agggtagcta acacacgaga tgtactaaca aatgcagcat
     3121 acaatgcagg caatgacagg atgaaaaaga tccctgaaac cacagaccaa ataagaaacg
     3181 cttttcttac aggactggaa acctctccaa atacactgat cacgctcttc atttgatcta
     3241 attcaggata atacaatcct aatacgtacg gagtcaaaaa atcactcagc acgcctaata
     3301 ataaaaagaa aaaaccaaat ttacgtaaaa aatccatgat acgcctccaa taacaattac
     3361 ctatgctttt aattgttatt ataaacgact ttatcttgat ttaacagaaa cctctattta
     3421 cacgaacatc tatataactc attaaaaact cgaatcattt taaaaatact aaatatctaa
     3481 cgttcaacgt tcacttaatc catctaaaca atctttcaat ttgatttttt ccccacttct
     3541 tttacgcgcg cctagctctc ctattgcagt aaacaacaga tcacttgacg aatttaaggc
     3601 agtttccatt gaatcctgga taacaccgat aataaatcca ataccgacga tttgcatcgc
     3661 gacatcatta gagataccaa acaaactaca tgctaatgga atcaataaca gtgaaccgcc
     3721 cgcaacacct gaagctccac aagcagctaa agcagacaaa atacataaaa ggactttcaa
     3781 aacaaaaggt gcttcaatcc ctaaagaatg tacagcagtc aacgtcatca aggaaatggt
     3841 aatcgcggca ccgcccatat tgatcgtagc acctaacggg atcgatatgg aataagactc
     3901 ttttgtcaat ttcaatcttt cagccaacat catgtttatc gggatatttg cggcagaact
     3961 tcgggtaaag aaagcgggaa gtgcgctttc tttcaaacaa aagaaaatca atggataggg
     4021 attttcttta ataaacaaaa acgtgaggaa tgggtacaca actaatgcga caaacagcat
     4081 cgtaccgatc aaaacaagta aaagctgcag atactctgcc aatccagcga ttccagtcgt
     4141 agcaattgat tgataaacca atcctaaaat ccctatcgga gcaaatgcaa taatcacttg
     4201 tacgacttgt gacaacgcta tagatatctg atccaccaac tcttttgtcg attctgaacg
     4261 tgctcgaaaa gctagtccca acaatacagc ccagaataag acgccaaggt aattcgcatc
     4321 aataagagat tgaacaggat tttgtgtgac attcgtcaac atagtagata aaacactttc
     4381 caaactttgc ggtgcttttt cagatactgt ttcttgtaaa gtaataggaa cagtaaaaat
     4441 tgaacttaca ataactgcta caagtgcaga aagcaatgtt cccactaaat ataagatcaa
     4501 aataggcttc acgaatgtct tattcccaat tttatgcttg gcaattgacg ccatcgtcaa
     4561 gaaaaaaacc agaataggtg cgattgcctt caagcaactg acaaacaacg ttcccaatat
     4621 tgaaataaat gtccatgtgg ggaaaaagat accaaacaat attccgacta aaataccaac
     4681 aacaatacgt tgaactaacg acatatccaa aaacttctct aacaaatgac caccctttca
     4741 caattttaac aatcattgta tcattttatg caaagaaact aaaggtataa ttttctaaaa
     4801 attctaacaa tacaaacaac catttcttgc taattttgta ctttttctgt ctacacgttg
     4861 ttttttgaga tattctaagt taggatgtta ttaatgggca acttatcagg aaacgttttc
     4921 ttatgttttt gtccaatttt tttgtgaggt gtttattctt gaaaaaaata aagaaggaga
     4981 gttatggctt cctgacaatc tttgtcgctt gtataatgat ggttttacag cgtatattga
     5041 tttcagcagt cactattcct acatatgcaa caaaccctgg tcgattctac atctatacat
     5101 ttttacaaac ttccgttgtt attggtgcaa atttgatccc actttacatg ggttaccaat
     5161 atcaaaaaat cgcccgttta aaagttttac attatttaag tcgttttgcc tttatattcc
     5221 taatagccac actagtttgc aatatctttt tttacgtaca agctggcagc ttgaatattc
     5281 gtgattattg gcttatccta acacctatca gtcagaatta ctttacttat gctgtttctt
     5341 gtactttgct tttttgtagt attccttata cagttggatt tttaaataaa ttatcgaaaa
     5401 caacaattaa atatctacta ttatttttaa caattggatt agttggctgc tctactctat
     5461 ttaataaaga cccgtttgca ttacagaatg gacagagtat tttatggatt ttttatttat
     5521 ttttaatggg ttatggtttg aaagaatgga actggaaaga aaaggtccgc tataagcttc
     5581 ctcaagtatt tatcagcttg attgttttat tcggtttgat tattttgatg acacaaattt
     5641 cactcatcat cagaggaaat acagatacag cgctgaggtt ttctaaacca tattctctct
     5701 tcgcaatgta ttatactttc tcttcgtttt tatttttaga aatgctatct gataaattag
     5761 gtataaaagg aagaggaaat tttcttgctg tatttttgat tgtcactcaa gttatatgta
     5821 atttcccttt agtttcttat cgcgtatcta catttatgaa acgtgaattt ccaaactcag
     5881 ggaaggcatg gctgataaat atcgggcagt tttttatttg atatttatta gcagtgtccc
     5941 tcctagttat ctttttattt tatatacaaa aaatccgttt cattcatcgt ttcatcgaat
     6001 acttcgcttt tgattcgatt gaacaattaa ctcagcgttg gaaaaaaaga attcattggc
     6061 tttttgttca taaaagttat tttttagttg cagcattttt ttactgcttt accttcgtgc
     6121 agattttcgt tttagaacca aaagaaggtt ggaaagaaac tatccaggtt gccttaaaaa
     6181 tatttactca gcgacaagca cccatgatcc tgacaatcat cattattatg ggatttttct
     6241 tcttactatt gttgatttcc aatcgttttt ggtacgcctt gactgctacc ttgatcatta
     6301 acttactgct cactatttcg acagtaatca aaatggaaat gcgggaagag cctgtctttc
     6361 cttcagattt aaaaatgctg accggattgt cagaactatt atcgatggtt agcccagtct
     6421 tattaatcgt gggcggattg atccttctcc tattattgat tactagtatt attgtacaac
     6481 gtagactaca aaaacagtat tccttgaaga ttcattggaa acgtcgattt atcagtatcg
     6541 ctgtattatt aggatgtttt tcgggtgtct ttttcattaa tcacaaaaat tcgccttctt
     6601 tcttgctgtt taatttattt aaagtaaaca agactttctt taaccagcaa ggcgccgtaa
     6661 aagataacgg accagtcatt caattcctaa acaatatcga cattaaaatc atggaagaac
     6721 ctgctggcta ttcgaaagaa aaaattaatg gaataatgaa aaagtacaac acaaaggcag
     6781 atgaaatcaa ccaaacaaga agtgactggt taaacaatca gacgatcgtt ctcaatttaa
     6841 gtgaaagctt cagtgatcct gcacgtgtcc ctaatctgac agtacctaca gaccctattc
     6901 cttacatcaa aagcattata acagaaggaa caggaggatt gatgttatcc gtcggttatg
     6961 gtggcggaac agctaacatg gaatgggaag gactgacagg cttgagtatc agtaacctgt
     7021 ctgcttcgtt agttacacct tatacccagc tagtggagcg tcagaaaata agtccaaata
     7081 ttaccgatct attcgatgaa aaaatagcca tccatccttt tacagctgct ctttatcgca
     7141 gaaaacaagt gtttgataaa tttggttttg acaggttcta ttatttagac agtccggata
     7201 aattaactta tacggataaa atagaacaaa gcccacgcat atcggatgat tcagcataca
     7261 aagaaacctt gaaagcttta caaagcaata cggaaacgtc acaattcatc caattatcca
     7321 ctatgcaaaa ccatatgcct tatgatgatt actatagtga attgaactac actgctgaag
     7381 gaactgctgt cattcctaat agaagaaaag aattacaaac ttatatgcaa ggccttcact
     7441 ataccgatac agcagtaaaa aaattcatcc aagaaatcga caaaatcgat aaaccgatta
     7501 cctttgtttt ttatggtgac catttacccg caatctatag tgggaatgat tcgaaaaaat
     7561 atggattaga acagcaccag acagattatt tcatttataa caacaagtat agtcgtgaac
     7621 aagcagctac gttaaataaa gaagttgttg ctcctaataa ctttggtgct atggctcttg
     7681 agcatgcgaa tatcaaagta actccttact atgcgttgtt gacagaagta tctaatcatc
     7741 tacctgcatt aacaattgat cctacagaaa gcttgtctaa tcgttttaat ggaaaacagg
     7801 tctttgttac agaggatagt caaattatcc aagaaaaaaa tttaactgag aaacaaaaag
     7861 aaattctaga agattatcgt ctgatacaat atgatcttgt tgcaggtaac cagtacagtg
     7921 caacatgggc gacacaaaaa ataaaagact aacaaataaa ctgagcaagg ggttgtgata
     7981 aaagacatgt cacaacccct tgtttatctc attaatttgc ttgtccataa acgatttgat
     8041 taaagccatt tttcaattca ttattcgctg tctcgttgaa gtaaagataa ttatcgggat
     8101 tcccctgatt ttgttgtgtc accaatactt ttggctgatt ttgctgaaac ccgaatagat
     8161 agacgtgctt tttcagatat ggttctgtct ctgcatcgga atagacggct gtgatttgat
     8221 agtctgcttg acctgtccca ctttctgacc attctgcaga aacaggcact ccaccaatag
     8281 ccatcttcca ctccccgttc aaaatagctt gagggacttt aagaccatat aagtccacac
     8341 tcatttgatt cgtataactt ttgtattgtt gtcccattgt ttggccccat ttggtcataa
     8401 atgcctctaa ttgtgcggat ttgtctgcat tccacagtgt ttgttcaact tgttcgctgc
     8461 tttctgaaga ggaagatgaa gcctctacag aagaacttga agtagatgtt tgagttgttt
     8521 cagtcgtttt ggaagactct gacgaagtag aggtatctgc tgtttctgta cttgctgcag
     8581 ttgacttgct ctctgtgcta gttgttgtag tagttgcaac tgttgctttc gctgtttctt
     8641 ttgttgattc ttttttcgat tcattcccgt tggtacaacc agctaacaaa aaagctagac
     8701 caattgttac taaaatcctt tttttcaatt aaaaaactcc tttcgattac tcccacttct
     8761 ttattctatc aaataagcaa tgtgatttta ttgatttttc gtattttctc atcaatcttt
     8821 caaatcttct aaaaactgag aagcacccgc tgatgttgtc tgtaatacat cagcgggtgc
     8881 ttcttttatt ttaatcttcg tgattttcca atttacctca cgctttatat ttgtatttga
     8941 agaaacatag acttttagtc atcatcgtct tcctcatcca aatcttgtcg gacagttttt
     9001 ccttggagtc tttgtacttg gtagatgatt tctttattga aacttaaaag ttcaacaagc
     9061 aattgttgca agccatattt ttcttctttt tcagctaatt tgattgcccg tgtgataaag
     9121 acattttgga aatcaaaatc ttttgcaaag ttttccaaca ttgttgaggc tggtaaatat
     9181 ttgttttgac catgttcgta aatttttcca taacgcatga cttcttctaa agtagacgga
     9241 acgatttcat tttcaatcac taactcatga gagattttat tgaaccatct cgcttcttga
     9301 tcagcatatt ctgaaaaaag ggtacttaat tcttgcgaag atgtacccaa aacatacaat
     9361 tgtgcttgga tcaatttcat ttcttctaat ttaaaatttg ctaatacatg ccccaccatg
     9421 gcgccagcag ttggtttatg atggtcgata tctgcttgtt ttgtttcttg aacaaatttt
     9481 tcttccacta tattcattga tttacgcctc ttttactttt gctgattgga cttcataacc
     9541 taatttttcc acaactgcag ctaattgttc agggtggact tcatctggat caaagtttgc
     9601 tttgattttg ctcgcattaa acaaaacttt cgtattgctt accccaggct gttgagaaac
     9661 cgcatgttcg attttttgca tacagcttgg acaagctagt gtatctaatt gcataataat
     9721 ttttttcatc gtagttcttc cttccttttt cttgatagtc ttattctaga ttacagcttc
     9781 cataaaaaaa ttgattctta tcaagtttta cgatttttct tctttgcttt cgtgtccaaa
     9841 atacaataaa cgcatcgcgt taaagataac gatcaagata ctagcttcgt gaacgaacat
     9901 cccactagcc atgtaaataa acccggcaaa taagccgttg agtaaaaata agacgacacc
     9961 aatggcaatc actacatttt cacgggtatt taaaacagtt ttcttagcca agcggtaggc
    10021 atgaaccaac gattcaaaat tagattgcat caagacaacg tcactcgttt cgatagcaac
    10081 atccgttcct gatcccatag caatcccgat atcagccgca gccaaagagg gagaatcatt
    10141 gattccatct ccaataaaag caacgcgcaa tccttgttct tggtattttt tcacaaattg
    10201 aaccttttcg tctggtaata attcagcatg gacttcatca atgcctaaca tatcagctac
    10261 ataatcagca gtcatctgat tatcacctgt cagcatcact aactgttttg ctcctgcttg
    10321 tttcaactta gctaattgtt cagcgacttc tggtcgaatc acatctgaca cacctatgat
    10381 ttgagtgatt tttttgtcga ttgctacaat aatgaccgag ctgcctgttt gttgtaattg
    10441 atgtaaatct tgtacttgct ttggtgtgag agaaagtttg tgagcggcaa tcaatttggt
    10501 gttgcccgca caaactgtat gaccatcaat ctcagctgtg attccttgtc ccttgattgt
    10561 ctgattatct ttgactgcta attgttgaaa atcaatgtct ttctcctgag cataatggat
    10621 cacggcgcgc cccagaggat gatcggataa cgtttctaat cgagccacta aagctaagct
    10681 gtccccttca tcctctgtat agttcttgat agtggataca gcagtagttc ctttggtcaa
    10741 tgtaccagtt ttatcaaaga caaaagtatc gattttggaa aaagtatcca ttgcttcacc
    10801 gcctttgact aagatgcccc gtttcgctcc attaccgatt cctgctacat tcgaaacagg
    10861 cgcaccaatg actaaagcgc caggacaacc aagaaccaaa acagtgattg ctaaacgaaa
    10921 atcacgacta atcagaccaa caaccaacgc aataagtaaa acggttggag tataatactg
    10981 agaaaatcga tcaatgaatt tttccgcatg ggatttggta tcttgtgctt cttctactaa
    11041 ttcgataatt ttggcaaagg tcgtatcatc tccgacctta gttgcttcaa tcgtcacgta
    11101 gccatctgct aaaatcgttc cagagaagac ttcatctcca ctgattttga cgacttgtcg
    11161 tgattcacca gtcactgcgg cttcatccgc ataccctttc ccgtcaatga ctcgtccgtc
    11221 cacaggaata gctccaccag tttttactaa gacgatatcg ccttcttcca cgtcatcgat
    11281 ttccacttct tcagtcgttc catcttcttg gacaacacta gcagtagatg gggccatctc
    11341 ggtcaaagcc ttgattgact cccgcgtttt ggtcaatgtt ttttgttcca gatagctacc
    11401 aaacaaaaac aagaatgtaa cgatagctga ctccttgtat tctccgatca taaaggcacc
    11461 aagcacagca atagtaacga gtaaatcaat tgagataact ttgactttca ctgcctgata
    11521 cgcatggatc ataataggta tgaccgcaat aatagaagct atggctagag caatgttata
    11581 gaacaagaca aaaccagtaa attttccgat aaaaccaaaa ataatcaaca cagctgataa
    11641 ggcggtgata tgatttcgat gctcttggat ccacttttgt aatttcatct ttctccctct
    11701 tttcctttca ttcttacgag actaagtata gatacttatc catcataaaa aattgaccac
    11761 tatcaagttt agtcaaaaag gcggttttct tttggtagaa tacactacaa aaaatagtta
    11821 gcaagaagac tcactaaaag tgatcttgcc aactattgtc gtttctatcg aaattttcat
    11881 tgaatgattt tcctgtacag catacatcgg agatctattt ctttatctaa gatttttatc
    11941 ttgctaattt tttcctttag tctttcaaat ccacgtcgtt catagaattg atacgtacag
    12001 ccactatctg taaaaaggaa tatttctttt cctttctctc ttttttcaaa ttctgttagc
    12061 aacttggtac cgatgccttt tgttttcaaa tcaggatttg cagctaaaaa aatgatctct
    12121 ccatctggcg aatgcttctt taaatatgct gcaagcattt gttcatttgc tgtctcgtaa
    12181 gctcctttac tttctttagc aaatatattt tgtaaaaaat cgaatatctt aacatatatt
    12241 tgctgtccaa aagaacgatg tttctttttt tctcctttta tctccgccag caaaacacct
    12301 gccaactcgt cctctgcata caaagcaaga atttgagttg ctcgattcaa ctccatatac
    12361 aaaaaatatc ttccgtaaag attcagcaga agttcatttt ccatatatct ctcaaagttc
    12421 attccttcaa cagcaaaata gatagctttt ttgtaatctt tcttttctaa gggtttaaaa
    12481 gttagtttca tcatttcgcc tcctcaactt caatgattgt gttatactta cattataata
    12541 gacacagtgt ctactatcta taatcaatca tctataagtt tgtcgattaa gcgacagctt
    12601 tgctttgata tgtcggataa ggagataggt aaatgaaaaa gacagataga cgcgtcaaaa
    12661 aaacagaaaa agctttggct gaaacactat ccacattatt agtaaataaa aagattcaag
    12721 caatcactat acgcgaacta acagaaacgg cagatgtaca tcgttcgact ttttatacac
    12781 attataagga tatctatgat ctatatgatc aattagaaag caactttttt cgggatctaa
    12841 atgaaattct ctcttatgat ccagcccata gctatgagga actatacact cgcttgattg
    12901 actatgttta cgcaaatact tttttatatc gcttatttat cctcaaccat ggtgatattt
    12961 cgtttcagaa gaaagttaaa caagttattg aagaaaacta tctgaacata tggctatatg
    13021 aagacaagaa aacgattatc acagaagaaa tgagattctt tactacgtac cacgtccaag
    13081 gttgtctatc cgttctcaat cggtgggtag aaagcaatta caagtactcc aaaaaagaat
    13141 tattagaact cttacagaaa ctgaatgatc acatagagaa aatcatgcct tgaataaaaa
    13201 catactaagc ttagttaaga agaaccgtaa ccttctcgta taacaactga atcttattta
    13261 gccaaattgt ttactgaaga ttttttgagt aaaatttcta taattcctac ttattgaaaa
    13321 gtagtttgta gtgctgcttc tccctctcta agtgatatat cttgttgtac caatcaaatt
    13381 cacctcatca ttaataaatt acttttaggc taatttatta caacttatag gttgaagctg
    13441 taaccaattt atagtcatat tatattttta tcatccagtt tattgaattc ttctcgttta
    13501 cctcaattca ttttcaaggg gaatcagctc atcatctaac attttacgcc atgctcgctt
    13561 catttcttgt gggtatttaa atggtttcgc ttctggatgt ttggctttat atccttcgta
    13621 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
    13681 cagattagaa gcatgtaatt ttttctcaga aacaaaccac tcattggaaa agacatgaat
    13741 caaatcatca cttacttgtg taaaatagcg acgtttaatg acaaagatat cttcattgct
    13801 atctagtgta ccttcatttc tttgtgctta aattcaaaga taattttctc aataaagatt
    13861 tttgattcca gtaagattcc tttactcctt ttacaacata gcgttgtcct ttgttttgaa
    13921 acatcttctt cccctctttg ccattaaaaa tagcccaatt ctctataaag aagattaagc
    13981 ttcattatta taatataaag tttaaatttg gtttattact tactttttct tattctctag
    14041 taagctaata ttgaaaaaaa tgtaatcaga tcattatttt tatatgttgt aacttcctga
    14101 taagaaaact aattaaaaga aagaggtaac tacataaact aattaaaaaa gctaacttca
    14161 tagttggagg ataaatggaa gaaacttcaa tgattgtttc tccttgtgtt ccttcaatcg
    14221 acactagtcc tattatcaat ctctcttttt tgtccattta cttttgcgac tgttccccaa
    14281 taatcaaata caggaagcgt tattttttgt cctttagatt catgtactgt atagactatt
    14341 ttcgaatcca tcacttgata aggcatagta tgccattcct tgccaattct tacttcatga
    14401 ttctgcaaat gttctttgta ttgtaatgct tccgtgggtg catagtcata aagtcctccg
    14461 ggaaggagtg tcgtgtaacg aataagattt tcttgattca ttaccaagtg atctctctga
    14521 gtgacaactt tatgttcata gtttgctttt gttgcagcag atacagccaa cagtaataaa
    14581 acaattgccg ttatattaac tacttttctt tgcttagctg atgtgctctt catgaaaacg
    14641 aacgataaaa tcaacgagcc aaacagtact tgaaagccta agattcgcca aggaaattga
    14701 atcatttgaa tgggtgtatt ttgtaataaa gaccatggaa aaaatttggt ggataagagc
    14761 caagagacta ccgcactaag gtaaatcact ttttctttgc gattaaatcc tttaaaaaag
    14821 aagagtggac agatcaaaga aataatacca atgattccta ccgtaaaggt tgtcaggtca
    14881 ttattcatcg caccaatcaa actatccgag acatttaatg cttggtgttg taaggtcgtt
    14941 ttatctggct cattaatcga ttgaaccatg atttgtcgga acatcggata ccaaaagtat
    15001 gctgttaaaa gaatcgtcat tcctactgcc tttgacaact ccacaatcaa ccggatgctt
    15061 tctttttttc ttgtttcact tactacaaag ccgcctaaga ctagtaagcc aacggctatt
    15121 acactcataa aagtggaaag aacatgtgta taaatgagta agctcatact gactgctaat
    15181 ggaattgcac atggcttttt ctcataaatc agtgcgtaag cataatacat aactatcggt
    15241 ataaccgtaa gtgcaatcgc ttcagcaata gcagaacgat gataaatatc aatcgtacgg
    15301 tacccataaa atgtatataa acagctaaat ataaaagccg catcgattct ttttaaaaat
    15361 tttagtccat aatgaaacat cagcatgata gtacaaacat tcaatagcca aacatataag
    15421 atataactaa cgattaaatt attacttatc caataaaaaa tgactgccgg aaaaaaggtc
    15481 aagtagggat aaaaataatt cactccgctc ccataatgat taaacgtcgt aaaattgatt
    15541 ggaccagaaa aaattgaaga taaccctttg actctagcaa aatggaaggc aatatccccc
    15601 gatgtagaaa tagtatgaac atttctaaaa taattgattg aataaagtcc agcaataatt
    15661 gctaggagaa tggacaatat tatttttttc tttacttgca ttacttaatt atatactcct
    15721 ttcatggatt gctttatcta gtataactta aattttaaat aaatcgttat ttatttttac
    15781 tctcacttaa aaaagcacta aaattataag agagggaggt aatggtctga acaccaagaa
    15841 ttgttccaac atcgaattgc ttaactaatg agttcaaagc ttaattatgt ggctcttata
    15901 tttaccgagc gtggattgcg atctctttag cgaaacaaac atctttcata aaaaaaacag
    15961 ccatatccgc tttcttggat atgactattt actactactt tactatcttt cattcttctt
    16021 gtctcactta tgataaggcg ctgcgtatca tgagtgaatg atattaggaa aacttcataa
    16081 aataaatctt ttatacattt ccacttctag atggtcattc atgttagaag aatcaaacta
    16141 tttttttact gtcgtaataa tattaaaaca ctagaacaaa tcaattaaat tccataaaat
    16201 ataaataata gacctagcat catctggtat aaaatggatg ctaggtccgt tatatttact
    16261 caaaattgat tctacgaata gatcttctta tcatttctat tttagtagtc atgcgttgtt
    16321 gttcttctaa aggtggtaat ggtataagta gtttagataa atttgtacca tttacatttg
    16381 gttgcccagt tccgtaagaa attttttcta ataacttcca atataatggc gagtctaaga
    16441 agcagtctac gtattctgat aataatttct caactaacct aattcgaatc aagtaagaag
    16501 caaaaacaga ttcctcactt atttccttaa ccaagaaact ttttcccata gttcctcctg
    16561 tccttgctat caaaatgtca ttttcttcca atcttaaatc aactagttta gaattactaa
    16621 tatcacagta aggcacactg gaccaattta cacgtccctc ttgtatatct gtgattctca
    16681 aaaacttaac attgccttgt tttttcgccg aatctgtata gccatattga atcttagtag
    16741 aaatacttcc taaataaacc catagtcgaa catcgctaga tgatttttct tgtcgttctt
    16801 tatgaacaac agtgatcatc ttgagttgtc cgactatttt ttagttgcag caatcttttt
    16861 gggacgctta tttgcatgaa gcaacgttgc taagtctagt aataaaacat tggctaacac
    16921 aggattggct ttctcgcgat acgctgcggc gccactttca aacaatactg ctagacgatc
    16981 ttctacaaca acgatttgtt ccaaatacgg tggaaatttg attcgtttta ggatccggct
    17041 aggagagtcc cgctcggaca ctaagagttc cgaggtataa ggaccataag aacgactggc
    17101 taagagataa tggccataaa aagccacacc ttgaatctta tcagatgttt ttctgctcat
    17161 tgtcacggtt ggttggattt tgtctttgcg gcgaattgct gttttcattt cagctatcgt
    17221 cggcaataga tagaaagtca actgtccttc accttttagt gtgaagttgc cagataccag
    17281 ttgatcattg ttttgagtca aatacgatgc ttgtggaatc tctgagagat cagttgtcag
    17341 ccaataagcg attggctgtc ccgtctcttc agatgtatct gctaagatat cgatcaaacg
    17401 taaagcggct actcgtccag tagcagatcc tgtgattgtc aaccataaaa gttcccgttt
    17461 tgtatcatat actaagcccc ctacatgtgg ccgtttaggt aaaatcagca gtttgatcgg
    17521 ctgtcctgtt tctctatcca gtacatgaat aatagaatga tgctgatggt catagcagta
    17581 ggcagaaata aacacaaaat cttgagaaaa agccaaccct tgcggcgtca tccattgaca
    17641 aacctcattt tcgccggttt ccggcaccac cattcttgtt tctgttaatc caggaatgtt
    17701 atattgtttt gcttcagcct tttcccaagc tgcttgaaca ataggatacc gttttaactt
    17761 tttttcaata tcctgatgta tccgttgctg cttccaatgc ttaaacttta ggacatagta
    17821 aacaagaagg ccaatgacca gcaacactaa aatcattatt gctacccgct gtgtcatttt
    17881 tttgcctcct tgttctattc gatcaatcgt ccatataccg ctattaatta ccgtaactac
    17941 atgtttcttt tactagcgtt tctttatagg atctttcata atctcattta tagtaacatt
    18001 aagaatccct tattctacta tagtcacttc cacatcaata ttgcctcttg ttgctttaga
    18061 gtatgggcaa aatgcgtggg cttcttctgc gattttctct gcttggtcct tctcaagtcc
    18121 tggcaaagaa acttctaaaa cgaccccgat ttggtagccc ccatcttcta attgaaataa
    18181 agatacccgt gctttgactt cagaatcaga ctcaagatta ttttcttcca gcatgtgttg
    18241 aaccgcgccg ttgaagcaag atgcgtatcc agccgcaaaa agttgttccg gatttgtccc
    18301 ttcactatgg attcctgggc ggtcgatctt catgtgcata cttccgtttg gtgcctcaac
    18361 ttcgccatca cggcctccac gattgatcat tgctgtttcg tacatttttt tcattgagat
    18421 catcctttct ttcgtcgttc tatattaagt gtaacgagta ttttttaatc tgacaaaggt
    18481 aatgcttgca ttcggcgaca tcagttagac aacatcgatg atcagtgata gaagcagttt
    18541 atctatacaa aaaaagaagg gttctatgat atttggtgtc cttactcgcc ttgagtaaca
    18601 acaccaaata tcatagaacc acttaatcag cttcacactg ctttttctac tttcgtagtt
    18661 gaaaaaaact taaacaaatc atcggcaaat agttaaagaa aaaacttaat cgttgaaaga
    18721 agcccaattg cttaaagaag gcaaactgac taatcgacgg aatccgtgct aatccgtaaa
    18781 gacctgcaat tagtaaacta ataactgtta aaacaagata aaaatggcta agcgtacctt
    18841 gaccactttg acgataaagc agaacaagta acaatgggaa aagtagaaag cctgcatagc
    18901 ctagcccaga acctatatta tggatccaag tcgatagatt acaagagctt tcattcgtat
    18961 tgatactgaa taagccagta aaaatacaat cacctatccc atataaacca actgttgcaa
    19021 caacaagtat tgcaagtgtt tttgatgttc caacaaatag gtgatacaac gcaggcaacg
    19081 aaagaacaaa aaaaagtcca gatacaactg accagaccaa gaaagcacgt ctgactggac
    19141 tatctacgtc accaaacaca ctaattacgg tagtcatttg attcagtttg ggataaaaaa
    19201 ggccaaggat atacggtgtc gataaatcac tcatcacacc taataataaa aaataaaaac
    19261 catatttttg caaaaaaatc attttcttcc cctacttttt agtgattatt cgatacatcc
    19321 aacaatcaaa ttttttctca ttttaacaca tatttttatt ctagttgtct tttgaacaat
    19381 caataacata aaggtgagtt tcttctacat tttaaaaaat cgaatggcac tgaagagttc
    19441 agttgtcagc atcaatctcc tactcaaaaa ggcaaaaaaa cgtaagtcag atgttgttga
    19501 catctcactt acgcatccat tgattgaaaa tttcttagta aaatctacta gcctaaaaat
    19561 ttgaagactt cttctcgatc atacagttta aatactttct cgcataaaaa ttgagtaatt
    19621 agtgccgcaa aaacagggat gactaaccaa caaagaacaa caagaaagcc atttagccct
    19681 gcatctaaag aagccaacgg accaacaagt ccgaccacgc cgaaaccagc agaagcaggc
    19741 gtacctgaaa tagaaaatag agcgacagga atagctgtaa tcactgcact aactaaacaa
    19801 ggaataagaa tcacaggttt tctaaataga ttaggcatca tcatcttcat agcgcctaaa
    19861 gcaatcgcaa ttgtcacacc tgacttatta accttccaag agttcactac taaaacaaaa
    19921 gtggttgcag ctacgcccat cgcagccgca ccagcagaaa caccatctag ttggattgcc
    19981 attccgattg ctactgttga aataggagag ataatcaaaa aggcaaagga acaaccaatc
    20041 aagatagaca ttaacagcgg ttgtaaggta gtgaagttat taatcaatga tccaattgca
    20101 gaagtaattg ctgttacaaa agggaataaa taaaaaccaa gtaaacctgc acctacacct
    20161 acaacaatcg gtgttaacac aatcgacact gacccaaaac tatttccaat cagtaaaatc
    20221 agcccgacag caatcgatgc tgtcagcatc gtattaataa tatcgcctgt accagcacca
    20281 acgtaaacac ccgtttcacc caaatcaggt gtgaatttga ctacaccaga accaacaaaa
    20341 gaagctccgg ctacaaccat cattctcatc ggatcaaatc caaattgtaa ggcaatcaaa
    20401 ccaccaatga tcaaaggtgt tgctagttga aaaataactg ctaactgtgc gatcgttacc
    20461 gcaaagggat acgcactaaa atattttaaa atggcaccta atactgcatt aggaatcaat
    20521 ccaataatga ttcctgttgc tgtaccagcg aggactttat tcagaaaaat tttcggtgat
    20581 attttttgtg aagtttccat tcaatctctc ctttatttta cgttcaaact atcttctttt
    20641 gcatggacaa gctcagtcaa gaaatcacaa taaggcgtcg caacaccata tttcttccct
    20701 tttctagaaa ttgcgccatt gatataatcg atttctgtta atcgatggtt cttaatcaag
    20761 tcttgataca ttgaaggata atgtaaccca attgtttccg ggtcaaaaca agattcacaa
    20821 tgtgcaatga cttctgggac atctagttca atcttctcta ctgctgctac tttggcaaac
    20881 tcattgacaa tcgtcgccac cattttatga gcagtcgatg tttttcctag ctcagccata
    20941 ttaacgtcta agattgtgca tagtccgttc attgttccgt tcacacatgc tttgcgataa
    21001 atagaatagt gaatattgtc agaaaaatga gcatttaatc ctgattcaga caatttgtct
    21061 gctaattttt ttgcagctgc ttcttttcca tcacctaaat tttgtaattc tactgaacca
    21121 cttccaaata atttgacctg accaggacct tcaagacctg ccgtccacat ggtatttcca
    21181 atatagatat tttccattgg tacaaatttc tcaataatat cttcatgccc gataccattt
    21241 aatagacata agacctctgt atcttttttg attaatgatt ggatatcctg cagcattttt
    21301 tccagctgca tcgctttggt aaatagaata atcagatcaa cttgatcttc tttttctact
    21361 tcggattgaa gaacgattgg tagttttgct tctacctctt taccattaaa atttgcttgt
    21421 aatccatgtt ctttgatttg ttggacatgt tctgcccagc catcgattaa taatacttcg
    21481 ttcccacttt ggtgaagcat caacccaaag cgacttccca ttgcgccagc gcctgcaatt
    21541 gctattttca ttgattcttc ctcctttttc tttctattat actcatttga gaatcttttc
    21601 acaacctcgt tttgttattc ttctcttatt tatcatgttt atatccacca ttcaagttat
    21661 tttatcagca aatcaataaa aactggaata gaatcccaat ttcatcgtaa gagaaactag
    21721 actagcaata agaaaataga aacattcata gaaaaaatct cactcctctg attataaaag
    21781 agggggcatt aacctcgtgt atactaaaac cttccattca ttcgatggat gattttcttt
    21841 ttgtgaaaaa atattcattg tagtacgatg ttgcgcaaca cagacggatg gtattgctta
    21901 ttatttatta aaatcgaaca atacacttat ttaaaaactt ctttcccaga ttttcttcca
    21961 tttttacagt tataactagt attatcatga aaaatagcct tttattgtct atgggaataa
    22021 taatttaaaa ttggtacttt tttattaatt agttcatcgc ccaataatct ctccagtttc
    22081 ttagcatctc ctgactcaac cggaattatc caagcccagt ctaaataatt ttttaaaatc
    22141 tcatcatatt ttgcaaatat ttcattgcca tattccgtcc ttaaaattat attttcagtg
    22201 ccagacaagt gatttatatt gagttgataa aaaagatctc catctaaaaa aatatcatta
    22261 gaagaagatt tccccacagt aatgaccatt ccatcagctc tatgtgttac atacaaataa
    22321 ttgtatgacc tcatttcacc taatgaatca attatttctt gccctttagc tctaatatat
    22381 tgatctctat tacctatctc actcttattt aacaaaagtt ttttcatgtc aaatttaatc
    22441 ggctgtttta tttttgtaaa ttcgcttcta ctactaatcc aatccgagat taatttttca
    22501 acatgtccca gagtaaactc tgatttattc gctaaatact ttttttgttg tattaaagac
    22561 atctttcccc gaagctcaaa attgagccta catcgattct ctatctcttc aaaaagcatc
    22621 ttatcttcat cattcttata ttcatcattc ataaaaccaa tccagctcct cgattaagat
    22681 aatattttaa tctcatacaa aaactagctc tcatcaaaac aaccattgtt ttgatttctc
    22741 atgaaatttc tttatacttt tctccgatta taaattccta accccttgag aaaattacgc
    22801 atatatttat ctccgcaagg cttaaaattt cgttggcctt cttttctcaa aaatgccccg
    22861 atttcgcctt ttgatggata gatttctgcc tcagcaaaaa cgtctaaaat atcatcagtg
    22921 gtcatagaca aggcgatttt taattttttt agcaacacat tgttgatata tttgatttcc
    22981 ttgctttttt gcggcgtgtc tactatcggc ttatcttttt gcggaccacg cgccaagaga
    23041 atcaggccat ttaggaatgc gtcaaaaact tggttgttga tggttttttc atacacatta
    23101 ttttctgcat tatctttgtt cttatcctgt actttggtca gtaccgcctg agcttcttct
    23161 ttagtcagtg gtacaccgcc tagctcaaaa gctttaatca aatccgtatc tttcaaatca
    23221 atcgcataac gcaatcgcaa taacctatca ttgttattca tttgttcctc cgaagtattt
    23281 agtctttact ttcttacgta ctttttgtct tgctaagatt tttaggatca atgataaaaa
    23341 ccgcatcact tatgataagg cgctgggtat catgagtgaa tgatattgct gaatacaaat
    23401 agtccgacca cattaagttg atcggacttc ataagcattt tattgactaa tacttttttg
    23461 cttaagaatt gattctaaca tcttttgcta aagttaagca tcctatagtt cctgcattat
    23521 ctgctaactt aggtgtgaca atgtattctt cgatatttgg cgtatcgaca tagccgttaa
    23581 ttagttctaa aaaacgtctt tgtactttct ttttcagatg ttcttgttgc atgacaccac
    23641 caccaaaaat tataatatct ggtgaaagca tcagggtcgt attacaggca cattgtgcaa
    23701 tataatccgc ctcgatttcc cagaacggat cttctataga aagatcttgt cccttggttc
    23761 ccgtacgcat ctcgatagcg ggtccagatg ccaacccttc gagacaatca tgatgaaaag
    23821 gacaagtacc agagaaagta tctcctgaat gtctcttaac taacgtatgc cccatttctg
    23881 gatggctaaa cccttcaaca aaaatcccat cttgtatagc tcctccacca attccagttc
    23941 caacagtgta ataaacaaca ctggataaac cttttccatg acctgaaaca tactccccgt
    24001 aacaagcagc attcacatcg gttgtccagg caattggaat ttcgaaatgc ttattcaacg
    24061 ttccaacaaa atcaaaattt ttccaggcag tttttggagt agaggtgata tgtccatagg
    24121 tctttgattc acgatggata tcaatcggtc caaatgaccc tactccaatg ctttctaatt
    24181 gttctttata gtgattaaag aaatcaatta ccaaactcat tgtttcattc ggtgtagttg
    24241 taggaaaact cactctttct aaaaccttta aatcatcatt gccaatggca caaacaaatt
    24301 tagtcccacc tgcttctatt gagccataca tttcttactc caccgtcctt ttatctagga
    24361 attcgttctt ccctgtctaa tagtttttcg aaaaactcat gtggttcctt ttggtaccaa
    24421 taggcaacag aagaaatatc gtcttgacgc tcataaagct tgatgtcatc atttcctagg
    24481 gtttggaaag taacacgtaa attttctttg aaactaatgg ggtcgggtaa atgccagcgg
    24541 tacaacccat gcatcggtaa agcatcatcg ccaaaaccat gaactggatt tggattaggt
    24601 tttccagcac taaaataatc ccgggtgtga tctcttttag attgaaaagg atagcccata
    24661 aatagcgtat taaaattttg agcatatgcc ctttctccaa agttcttttt atggaaagcc
    24721 caagcgccgc caaaataatc ttctgctcct gtagatgtta ttgtagggaa atcttcatct
    24781 ccatctatat aaaacttaaa ttctccttcc ccccaccagt atctttccaa tgcgcacaaa
    24841 gcaaagtagg ttcctacata atacccagtg cctttgattc catcaataag ggtgtaatct
    24901 tttccttttg tcgtgatggg ttcacggttc catttagcat gaaaatacat cgcatcatcc
    24961 ggtacttcag tcaatgcata attaaatgta taaaaaatgt gcttaataaa ctcaggatgt
    25021 tcattcgtta aagtaatctt agcttttttt cgaaatggca tctcaaaata gcaattcatc
    25081 cctgatgttg gattcactac gattggtaag gaattcactt cgcatcttgt cccaaaccca
    25141 ttacaaaaaa aatctcctaa tggactttct acagaaggag tggattcatc atcccaataa
    25201 atacgcaaaa ttaaatcacg taagacgaaa ctcccagcga ttgttttgtc tgtcactgtc
    25261 atccaaaaat gacgtaaaat tccagggcct tccatttctg ctaaagtaat tgtctctcca
    25321 gcatttatcg gaacagaagg tgaccctttt ctcccaattc ctagatgact cgctgtcatt
    25381 gccgctttcc ctttttcacc atttttattt tctggtgaaa tagttctact ttcctctccc
    25441 ttgtggagcc atatattatt taaacaattc attttattcg tcctctatcc tttcttcact
    25501 tttttgatta ttcatataga tgatttgttg ttgtgcctcc gcaataattg ctccaatatc
    25561 taaattatca tttcctaact gcaaagcgat tgacattgcc aagggaagat tcattcctgc
    25621 aattacgtgc actctgtcag aaatataggt aaaaaacttt tgatttacac ttcctccagc
    25681 catgtcagtt aaaacaatta tttcgtctgt agtatccact aactctttaa tttgttcatc
    25741 tatatcatca atttcttcga catatgcaca taagctacta atttgattat gcatacctgt
    25801 taaaaactct agtgtctctt taaaaccctc agctagtatt gaatggctag caatgatcaa
    25861 tcttctcata attcaaatcc ttttccttta cgctaatatt ccaaatgcag ccataaccat
    25921 cgcaaaaaca ataacgagta gaatcaaaat ggtcatactg atttttttct tactgagtag
    25981 ataatatgct gcagcagtaa taattactgg aattaatgtt ggtaaaattt tgtccaacat
    26041 tggttggatt ttcatactaa cttcaccaaa agtgaattta agtggcgttg aaacactgat
    26101 aactgaagca attaatgatc caactactgt tagccccaat actgaagcag cttcagttaa
    26161 ataattaatc ttttcaccgt attcagtaac aatcttcatt ccctgcttat aaccaaattc
    26221 aaagcctctc attcggaaaa agaagattac catgattaaa atgaaccata aaaagattcc
    26281 tacaggatta ttttctaaag ccatataagc cgcaattgat cccatgatgg ttgggatcat
    26341 cgtcatcaaa agagagtccc caataccagc aattggaccc atcgtaccaa ctttaaagtc
    26401 attaatcgca tcttttgctt ctaatccttg cttttcctcc attgcaacac aagcacctaa
    26461 aataatggct gccagccaag gctgtgtatt gtaatactga aagtgattat ttaaagcttc
    26521 gatgtattca tcatcatctt tgtataattt tcttaaaata ggtgacaatg cgtaagtcac
    26581 agatgcacct tgctgtgttt gataattaaa ggtacaaact gacatcaacc aacgatagtt
    26641 cgctttccta agatcctttt tcgttacttg atatttttcg ctcattttat tcatcctccc
    26701 caataaaccc aacatcagaa gctcccgcag aagcagtgat ttgtgcatct ttttcagctc
    26761 tatgatacac attaaatgct aaagcgaatc ctatgatcgc cgctccaata ataggtacct
    26821 tcaaaaaagc ggctaagaaa aatccaacaa gtaaataact aatgtatcga cctgtaggca
    26881 taaagcgtaa caacatcgca ataccaacag caggcaacat ttttccggct aatgttagcc
    26941 catccataaa ccaattaggt aaaacatcca aaacgtaatt aacagcagac tgtcctaaag
    27001 taacggcaat aaatactgga attgctgcag ataaaccaaa gaaaattggc cccaaccaca
    27061 aaacactatt cattttctta aattctcgtt tattcgcata ttcctgtgat ttgcgaacaa
    27121 caaaaccatt taagattttg acaattacat ctagttgcac ccctaacata cctacaggta
    27181 gtcctattgc taatccggct tctactccaa gctggctacc aaatgccgtt gcaataattg
    27241 cgcacgttcc atagtccggt acagatgagc caccaaaacc ggcaactcct aatgacatca
    27301 attgaactgt accgccaact atcaaacctg tttttatatc acccatcaaa attcctgtga
    27361 ataatcccca aaaaactgcg taatttacaa agatcatcgg aatcccataa taatcaacat
    27421 gtttaataaa tgctaatata gtcagcataa taatttgaaa aatagtaaaa tgcattatcc
    27481 ttactccttt atcctaattt tgattgaatt tcctgtgcct tatccgccgg aacaaattgt
    27541 gtaacaattt ctacaccaga atccaacaat tttttatacg tgctgatatt ttcatctgat
    27601 gcataggctc tagaagatag ctgtaatgca ttatctttaa caacagtacc gccaacaatt
    27661 agtcggtcaa ttctaatacc gttatctact aaatgaagga ttgtttccgg ctctttcacg
    27721 atgacaaata ctttttcctg atcgtatttc ccctttttaa aattcgcaat cgctgtttct
    27781 tcagtgataa ttgatacagc cattccagca ggtctagcta aacgcatact tgatttttgg
    27841 atgtcgttat ttgcgacaga atcatcgatc accatcactc tgtttgcttt agacattggt
    27901 gcccattgcg tgacaataat cccatgcaat aaacggttat caactctcga cataacaata
    27961 ctcataaata cttcctcctt tatctttcat tttcttacaa gcaattacca tgccaaaaca
    28021 aaaagaactt gataaatcaa gctcttttta cataatcata aacgtattta acctcatgaa
    28081 tcgggatgtc gacactataa taatgttcaa cgacactgaa agattgctta aaatgtgtct
    28141 gaaatttttg aaattcctca ctcgtttcat tgaaataaat ttcatcttcc aaagtatttt
    28201 gttttactaa tcgttcaatt aagcaactaa tgtgaacata taatccgaaa cacgtattat
    28261 tatcaaatga aattcctaga t
//
LOCUS       DAZULO010000025        27796 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000025 DAZULO010000000
VERSION     DAZULO010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.026"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(335..1279)
                     /locus_tag="ACG8OA_001121"
     CDS             complement(335..1279)
                     /locus_tag="ACG8OA_001121"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS4975947.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1575..2519)
                     /locus_tag="ACG8OA_001122"
     CDS             complement(1575..2519)
                     /locus_tag="ACG8OA_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS4975948.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2739..3953
                     /locus_tag="ACG8OA_001123"
     CDS             2739..3953
                     /locus_tag="ACG8OA_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4975949.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3975..5990
                     /locus_tag="ACG8OA_001124"
     CDS             3975..5990
                     /locus_tag="ACG8OA_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFS4975950.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6073..7446)
                     /locus_tag="ACG8OA_001125"
     CDS             complement(6073..7446)
                     /locus_tag="ACG8OA_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS4975951.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7472..7756)
                     /locus_tag="ACG8OA_001126"
     CDS             complement(7472..7756)
                     /locus_tag="ACG8OA_001126"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4975952.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7758..9773)
                     /locus_tag="ACG8OA_001127"
     CDS             complement(7758..9773)
                     /locus_tag="ACG8OA_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4975953.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9952..10257)
                     /locus_tag="ACG8OA_001128"
     CDS             complement(9952..10257)
                     /locus_tag="ACG8OA_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFS4975954.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10346..11377)
                     /locus_tag="ACG8OA_001129"
     CDS             complement(10346..11377)
                     /locus_tag="ACG8OA_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4975955.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11584..12459
                     /locus_tag="ACG8OA_001130"
     CDS             11584..12459
                     /locus_tag="ACG8OA_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4975956.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12611..13453)
                     /locus_tag="ACG8OA_001131"
     CDS             complement(12611..13453)
                     /locus_tag="ACG8OA_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4975957.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13545..15422)
                     /locus_tag="ACG8OA_001132"
     CDS             complement(13545..15422)
                     /locus_tag="ACG8OA_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4975958.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15419..16840)
                     /locus_tag="ACG8OA_001133"
     CDS             complement(15419..16840)
                     /locus_tag="ACG8OA_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4975959.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16843..20232)
                     /locus_tag="ACG8OA_001134"
     CDS             complement(16843..20232)
                     /locus_tag="ACG8OA_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4975960.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20489..21880
                     /locus_tag="ACG8OA_001135"
     CDS             20489..21880
                     /locus_tag="ACG8OA_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4975961.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21953..22600)
                     /locus_tag="ACG8OA_001136"
     CDS             complement(21953..22600)
                     /locus_tag="ACG8OA_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4975962.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22608..23294)
                     /locus_tag="ACG8OA_001137"
     CDS             complement(22608..23294)
                     /locus_tag="ACG8OA_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4975963.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23300..24373)
                     /locus_tag="ACG8OA_001138"
     CDS             complement(23300..24373)
                     /locus_tag="ACG8OA_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4975964.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24595..25269
                     /locus_tag="ACG8OA_001139"
     CDS             24595..25269
                     /locus_tag="ACG8OA_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4975965.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25274..26614
                     /locus_tag="ACG8OA_001140"
     CDS             25274..26614
                     /locus_tag="ACG8OA_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS4975966.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26763..27749)
                     /locus_tag="ACG8OA_001141"
     CDS             complement(26763..27749)
                     /locus_tag="ACG8OA_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFS4975967.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8096 a         5253 c         5046 g         9401 t
ORIGIN      
        1 tgcagttttt cttacttttt ttgtcgttta attgaatgat aaaaaccagt ttctttattc
       61 agttaatcaa atatgaacga attagatatg aatcattgta tttgccgttg cggaaggatt
      121 ttttaattaa ccacagataa acattgataa cgattcgctt attgtaatca acagattaaa
      181 tttcatatgt tcatatgtac ggatacattt tcggaccgat taattttaac cattctgttt
      241 atttgcttac ctattagata tcaataaaaa actgcttaac agctatttca gctgttaagc
      301 agttttagga tttttagttc atattcattg tttcttataa tacagtgttc aatacttctc
      361 ttaaagcact tgttgatttg ctgaacattg ctttttcttc ttcgttgatt gataattcaa
      421 caacttcacg aacaccattt tcgtcaacga ttgatggcac acctgtaaag atatcttttt
      481 cgtcgtattc accagttaaa taagcagata caggcaatac agcttgttcg ttattcaaga
      541 tagcttttac gatacgtgca gtactcatac caataccata gtaagttgct tttttacgat
      601 caatgatctc ataagcagca ttacggactt tatcagcaat cacatctaat tcatcttggg
      661 caatacgatg gtctttttct acaatttcaa acactggttt accaccgaca gttgtatgtg
      721 accatgcagc aacttctgaa tcaccatgtt cacctaagat atagccgtgg acgctgcgtg
      781 ggtcaacttt taatttcaat gcgatttctt tacggaaacg agttgtatcc aatgttgttc
      841 cagtaccgat aacacgtgat gttggcagac cagattcgtt ccaagcgata tatgtcaaaa
      901 tatctactgg gtttgaagca acaacgataa taccgtcgaa tcctgatccc atgatttctt
      961 ttacgatttg gcgcatgatt gacgcatttg ttttaactaa atctaaacga gattgaccag
     1021 gtttttggtt gataccagct gtgataacaa cgatatcagc atctttacat tcttcatagc
     1081 cacctgacca tacagccaca ttttcatcgc cccaagccat accatctaaa aggtccaatg
     1141 cttccccttc tgctttttct tgattcacat cgatcaatac taattcatta gagattcctt
     1201 ggttgatcat tgcataagcg atacttgttc caacaaaacc tgtaccgacg attactactt
     1261 tacgacttgt ttttttcatg aataaacagc tccttttcat aactatttgt tactttcatg
     1321 aaaagtatag tcttgtttat tcattttatc aacatgttaa ttataatagt tgttattata
     1381 atttttgatg gaaacgaatt cctgaatacg cataacagat aaatgacaaa tgtttacacc
     1441 tatatcccct gataatacaa ccaaaagttt agaactgaaa ataaatccaa aaaaattcgc
     1501 tgcaacgata taataagaca gcaatcgcac ctaaaacaat tttccttcat atgctttagt
     1561 gaaagatttt cttcttaatg tttatttctc cattcattgg gagtacatgt tgaatacttc
     1621 ttgaataatt tgaataaaga agtcgtttgt ttatatccga tacgattagc gatttcttcc
     1681 aacgaaaaac tcgtttcttg aatcagttcc aacgcaaagt cgaatttttt ctttagaacc
     1741 agttcttgaa aggaagcatc aaaatacatt ttcaatttat tactaagata gaccggattg
     1801 tagccaaaat gagctgctgt ttcttttaac gtacaagaag taaaatgctt atcgatataa
     1861 tctaatactc taagcatctc attttctgcg catccatctg ctgatgaaaa gctttgttgt
     1921 tctaataaat gagtcaattc gatcagtaat agtgatgtat agagatttaa cgaacggatc
     1981 ttatgactgt atgtatcaaa ataaatcaac agcatatttt caatcaaatg cgtcaaacga
     2041 cttgtttttc cgccgcgaaa atgaatgaat tgtgtatgtt cccctttttc ttttgatgca
     2101 tttatcaaaa aagaagaaag tagattattt gtgcttgcca aatgattcaa tatctctgta
     2161 gataaggttt cttcttttaa taagatgttg attaaaatat cctcttgccc tgtgtattcg
     2221 attctttgag tagcttcttt atcaatgatc accacgtcac tttcttccat ctctatcctt
     2281 tctccattaa taatttgtaa acatgtaccg ctatacatat aattgatttc aacaaaatca
     2341 tgtgtatgct cgggaacttc cgcatatcga cggtgtttac tgatatgaat atttccctgt
     2401 tgaaatagat cccctcggtg gatttttggc ggtgtctgaa gatggtaagc taagtttaaa
     2461 tcattgatat tcattccttg ttcacgctgc agagtttcga ttgattcttc gttacgcaaa
     2521 aaaatgtcta attcagctag ttccaattga ctccctcctt tttgtaagcg gtttctttcg
     2581 cttattagtt cacgctgtac tgctattata tgcaaagcta tttttggttt cataaagact
     2641 ttctatggtt gggacatgaa cttcctgtga ttccgctatt gagagaataa ccacttcata
     2701 ctatattttt gataagcgct tacaaaaatg aaaggaacat gcttatgaat aaaaagatga
     2761 tgaaattttc tctgctgtca gtctctctta ttttgacttc tgctggtgca gtcacgtctg
     2821 caattcccag tatgtcagct acttatgcca cgcgttcttt atctgctgtt gaattattaa
     2881 cgactgctcc ctcttttatg gtcactattt ttgttttgat tagtagtttt attgctagga
     2941 aaatcggaca aaaacaaact gtgatcatcg gattagtgat tgcatcgatt tgtggtatcc
     3001 tccccctctt ctcacaaagt tacagtattg tcttagcttc aagagctgga ctaggtattg
     3061 gctttggatt gatcaattct ttggcagtca gcatgattgc tgccttttat cacggagatg
     3121 aacgagcctc tatgattggt tttcaaagtg catttcaagg atttggtgca gctttaatga
     3181 catttgttgc aggacagcta gtacagttcg gttggcaata taccttttta gtctacgcca
     3241 tcagcttacc tattcttgtc ttgtttgttt tgtttgttcc tacacctgaa acaactgaac
     3301 ttcaatcgca tggcacagcc ccaacaagac aagtaatcaa aacgaatcct aaaatgatta
     3361 gttatggtat ttttctcttg ctggtcatcg tggtatataa tgctgtatcg attaaattag
     3421 ccacagtcct gacgacttat tctctaggaa acgaaactaa tgcagcgaat atcttaacaa
     3481 tcatgcaatt ggcaagtatg gctgccggag tttgttttgg ctggcttcag acaaaagcac
     3541 atcgctatat gcttacactt tctttactac tgatgggaat cggctttgta ttgattgcca
     3601 ctgtgataaa cctgtatgtg attgggtttg gtgcgatcct aacaggaatt tctttttcgt
     3661 tattcattcc ttacttattc aaccaagtaa gtatccaagc gcccaagtac gcggagagtt
     3721 tcaatacttc aatcttattg gttggcgcta atttaggtac tttcattgca ccatatggtt
     3781 tgctctttct ctcttttgct tctcttggga gtaaaacact tgctgttttc gtcaatggca
     3841 gtatcttgct gttgattagc gcgcttatta gccttctagt agtacacagg acgaatagaa
     3901 agacagtgat aagagtagat gaagaaataa aagaacaatt gacaacaaaa taaatttgat
     3961 ggaggtcgtg taaaatgaca aatgaaaaac ataaagatag atggctttgg tatcctggag
     4021 actttgagat ccgccatgga ctgctacaaa atttccaacg ggaagaacga ggttttgact
     4081 ggccagcgta ctggtacatg gatgattgcc atcggaacgt caagtttaag agatattact
     4141 ttcttgatca accttccatg tttaaagtga cgatccaagg agttgggtat gtagaaatta
     4201 atggacaaaa acatccatgt ggaaaatggc tgacttgtcc agctggtaaa gcaaaaattc
     4261 gtatctttgt aggacataca tcagggctac cagcgatgtt tatcgaggga gacgaagtaa
     4321 aatcagatat tggctggaca gcaagtaatt ttattgaaga atatccagct ggctggagtc
     4381 cgctttatgt cgatatcgca aaagatccaa atcaaatcta ttatcaaaaa gaatgcgtcc
     4441 atccagtcac tgaaacggag attggcggag gaatcttatt cgattttggg cgagcagtga
     4501 atggctgtgt agaactaata tctctggatg accatatcga atctatcact ctatgttatg
     4561 gagagtccga tgtagaagct atggatatcg attattgcta ctataagcaa ataggaatga
     4621 caagaaaaat gaaagctcgt aaacgcgctt ttcgctattt gtttatacca gaggtttcta
     4681 ctggaatggt tcaacttcaa gtttttcatg aatttttatc tctttcgaaa caagctgctt
     4741 tttcaagcga ttcttcttta ctgaatcaga tctggtcagt atcagaagaa gccttcaagt
     4801 tatgtagtga tttgtttttt atcgacggta tcaaacgaga tcgctggatt tggtctggag
     4861 atgcgtatca atcattcatg atcaatcaat atcttttttt tgacgaggaa atcaataaac
     4921 gaacgatatt agcattaaga ggacaaaatg aaatcaagca gcatatgaat actatcgttg
     4981 actattcgat cttgtggttg attggtatcg aaaaccacta tatgatgacg aaagactatg
     5041 aatttttacg catcgtctac cctaagatgc aatcattaat gagatacttg atggagcaaa
     5101 cgaatgaatt agggtttatt tatgggagag caaaagattg gatatttatt gattggtctg
     5161 aaatggataa agaaggaacg cttgctgcag aacaagtctt attactgaaa gcctatcatt
     5221 ccatgatcac ttgcggagat atcttagctg aaacatatgg agagaacgaa aattatcata
     5281 ctcatctcta tcaagaaaaa tatcaactat tgaaacaaaa cctatttgac tacttttggg
     5341 atgaagaact tggcgctttt atcgattgct atgaatctgg gagaagaaat gtgacgcgtc
     5401 atgcgaatat tttcgctgtt ttattcgatt ttgctaacaa agaccaacag caatcgatct
     5461 taaagaatgt cttattgaat gacactatca cacagattac tactccatat ttcaaattct
     5521 atgaacaaga tgctttatgc aaactcggac agacgaatat cgtctaccaa acgattcttg
     5581 attattgggg agggatgtta tctcatgatg cggtaacttt ctgggaagaa tacgacccta
     5641 ctcaaacagg aaatgaaaag tacagtatgt atggagatcc ttatggaaaa agcttatgcc
     5701 atgcatgggg agcaagccca atctatcttt taggacgcta ttttatcggt ttgtatccaa
     5761 caaagccagg ttatgaagaa tttatgatac aacctaatct agattcattt gaaacactga
     5821 attgtacgct acctatcaaa gatggaaaga tttcgcttga aaaaacaaaa gataagatcg
     5881 tgattacttc tgatagaaca ggcggaacag tgaaaatgaa tggtaaaagt tatcctcttc
     5941 tagcaaaaca gccacttgtc ttaacaagct attcaaaaat aaatgcataa aacaaaaaac
     6001 tgcttgcgac atatcagaag actttaaatc ttcttctgat attgtccaag caatttttat
     6061 tttgaagttt gcttaattct gttctttaga aacaatgttt gttctttttc cggcaaaaag
     6121 aaagctagat aatacgacaa gtaaaaagaa agcgatgatt ccaactgtca aggctatttg
     6181 accgtattct cctagtttcc caagtacaag tcctggtact agataatcag tatcggcaaa
     6241 tgtagaagaa gcgattttaa gatctcctaa tactggaagg ataaataatg gcagccaact
     6301 aatcaataag ctattgaaag cagaacctaa taccgcccct cttctaccgc cagtcgagtt
     6361 gccgaagaca ccagaagcag ctccgcagaa gaaatgtcct actaccccag gaatgataac
     6421 tgtagtttgc aagcctatca tagcaaacat ggatagtgtt cctacaaaga agctaacaaa
     6481 aaagccaatt aatacagcat taggtgcata tggaaaaacg ataggaacat ctaatgctgg
     6541 tttagaatcg ggtacgagtt ttttagcaat cccttggaat gctggaacga tttctgccaa
     6601 caccattcga accccttgaa ggacgataac aaacccagct gtgaatgttc cagcttggat
     6661 cagtgcaaag attacagcgt tgtctccatt gcttagttct ctttcaacaa atccaggacc
     6721 agctaacaaa gcaagtgcga catatacgat cgacatcaag agcatgatcg atacagtaga
     6781 atctcgcaaa aagctgaaac ttttaggaat ctttatgtcc tctgtagaac ggtctttgtt
     6841 tccaaaaaac tttccgatcc atccactaga agcataaccg atatttccgg tatgccctaa
     6901 cgcaacttga ttgtttccag tgatgttctt cataaacggt tgacaaagag caggcgtaat
     6961 agttaatagt gttccttcga agaccccgcc taataagatc atccacaaat ttgcagtaaa
     7021 ccccgcgctt actaaaatga ctgctgttag acaagaaaca taaagcattg cttgacctgt
     7081 aagaaaaatg aatttatatc ttgtgaatct agcaagtaaa atattcacga tcatccctac
     7141 tagcatgatc aaagcagtag gtgtaccgta ttgttgcaaa gcaatcgcca ctactgcttc
     7201 attgctagga actactcctt gagtatgcag agctgtttgg aacatcgaag caaaaggggt
     7261 tagtgaatta ctgattaaac cagcaccgcc gatcagtact aggaatccga caaatgtttt
     7321 gatagttcct tggatcactt ctgaaaattt cttcttctga agaacgagac caagcagtgc
     7381 aatcaaagaa acgaggatgg agggctgact gacgattgac acaaacaaat tcaacaattt
     7441 atccattgat agttcctccc ttgaatcatt tttattcttt tgacaagatc ttcgttactt
     7501 tttcttttac ttcttcgctg tcaatgatcg attgcaaagg tacgagcttt tcatcaggta
     7561 tagaagttag cgtatctttc aaagaatgct caatgaagaa ataatcagcc atatcgctag
     7621 atacacttcc aacatccgta tgacttactt caaactcgtc cacatctgct ccaagatctg
     7681 caagtgcttg tttgatgttc atctctacca taaaacttgt tcctaatcca ctttgacata
     7741 ctgcaaaaat tttcatatca ttcgtctcct tttcttatga tcgctaatat ttcttctttt
     7801 gttgatgcgt gtaataaatc ttgaagattt tgtttattag aaagtagctt tgttaaattt
     7861 gccaaagccc gaagatgtgc ctcattatcg atagcagcta aacagatgaa caaactaatg
     7921 ggatgggctg gattatcaag cagatccaca ggttgttgaa gtttcagcaa agacatccct
     7981 acttcgttta cgccatcttc tggacgagca tgaggtaatg cgatgtgatc gccaatatgg
     8041 ataaacgcac catactgctc cacgcgctcg atcatagctt tgatataact ttcttctatt
     8101 ttattttctg ataatagcgg ctgtgcggca aatgtgattg cttcttgcca gttttttggt
     8161 tgatccgata attggatcat ttcttctgtt aataattcag atagcatagg acgttgatcc
     8221 tccttctttt ctaaaagttt gttcatttta cgatttaata cttgatagag cttttcttcg
     8281 tctacgcctt cttttaattc gatataaggc agtagcgccg acaacaattc ttgggcagaa
     8341 ggcattgaaa atcctggtat cagccaatct ctttgtactt gattgatcag ctgatttttt
     8401 tccaagtcag tcatgattgg atgcagaaca tagagagact ttttcttatc agtaagtgtg
     8461 agaggtacag tagaaaaaat aatatcgtaa gaagtttcgt caacctcgtg taagcggtca
     8521 gctgaattag tctctgtaaa aaagatcatc gggaataatt gctgaagttc actttttaag
     8581 atcaatgaag agctaatgcc acttggacag acgatcacgg ctcttatttt tctatttctt
     8641 tcttctgcgt ccgcttttct gatctctccg ccaaatagta tagtgaaaaa accaatctct
     8701 tcttcaggga tgctcttccc tattcgtctt tctaacggaa gtaatgcttt tcttgtcatt
     8761 tcgtagatgg aagcatattg atgcttaatt tgatcgatca tcacattagg taaataaaaa
     8821 gaataggaaa tacgaaaata ggcagggacc aagtgataaa agacattcat caataattca
     8881 cgaaacgaag caaattctac ggccatcagg aattccatgc gatggatgac ttctgaagaa
     8941 cattccagca aaaaatcaag tgcaggctcc tggacatctc cttcaacgat cgatgctaag
     9001 caaagagaga aaaatataat ctccgaattt tgatgttcgg aatagccaag gccagtcaat
     9061 acttgtcgac taatgtcagt gactgcaaga gaagccaaca gttcagcatc ttttaccagt
     9121 tccatggatg tttttccaat tcgaatggtt gtcaaactca aataataagc taattcatcc
     9181 attcgatttg gtgcgaatcg aagatctgat agtgaaatcg ttttatctag caaatctcta
     9241 actttggcgt aataatcaat ggaaagggtt gcggttaaat acgtgagccc aaatcttcca
     9301 gcaagtgact gcatcaaacg agcagtgcga ttttttgctt ctcttcttaa tattgcttct
     9361 gaccctagca gcctaaaacc agttttccta gtatacgaca gctgacaatc ttccacttct
     9421 aattcatttc ttatcttttt gatatctgac aggactgtcc ctttagaaac ctgaagaaaa
     9481 tcttgaaaat gaaaaacaga aacagacgtt gcagtcgaat aaaccatcaa ataaagaaga
     9541 tctcttcgct cttgttcggt atacacagct tcaaatcgag taatatccaa aaatttttct
     9601 gcccagtcga ttgctcggaa tttctttata cggatacggg tatcatccac atattcggca
     9661 aaaggagtta attgtttgat cgcctcgctt gtatttactt cgatgatctc tgctgctttt
     9721 tctagactga tttctcttgg tgttttcagg agataactaa ttttgtagtt cacagcgctg
     9781 ttcacctcct acttatattc tagtatggta gcgcttttaa atcgaggggt gtttggtaca
     9841 acttacagtt ggattttttt tacgactgta ccgaacgatt tctatcgaat ggatacagaa
     9901 aaaaagcagg tgaccaaatg tctcctgctt ttatgaattg atttcgtccg atcaatgatg
     9961 atcatgttta ttttttgagg tcagttcttc aaatttttct ttgacatctt caaccgcttt
    10021 ttcgatatct ttttcaaagt gattgggctc attaggcttt gtttcctctt ctacagaatt
    10081 tgaaccagat aaagctgttt ctttattttc tgtttcacca gagtttgtgc cagctaatga
    10141 ttcttctttg tcttctgttt caccagaatt agttcctgct aaagccactt cttcttctgt
    10201 tttaggggcc attgaacctc ttaatcgttt gtgtttttca ccgtcataat ttgtcatgtt
    10261 ggttcctcct agtagttcta tactttttca tcgtacaata agacagttta tgcgtcaaaa
    10321 aacaaacccg attcttacaa atatgtcata tttttccttc tgattcttga atgcttttga
    10381 ttccttcttc caaaattttc atttgtaaga ttgtttcttc atctttgatg actttttctg
    10441 cgccattaac taatgtctcg taaatccctt cgtagacacg gctgtaatca ccaacctcgg
    10501 aaattacttt ttcttcatga taactccctt gttcatctat ataggtcaat acaccataat
    10561 gttcgggcaa atcgatacca aagtcttcat gatcaggcat gtaaaacatt ttcagatgtt
    10621 cttcttgtcg atcttttgtt tgtttcacga acatcccttt ttttccgtaa acgacgaaac
    10681 ttggacgttc tttgatgcgg aaataactgg attttatcga aattttcata tttccataga
    10741 aaaaatccag atcgaagtaa tcattcattc gtcctttccc taaaagttga cgtacatcat
    10801 agctgatatc atctggttcg ccgaaataag aaagaacttg atctactgta tgacaagcat
    10861 gtccatataa atagctgttc gcagtagaaa attcatgcgt attttctggt acttccggtc
    10921 tgaaataatc ataatgcatt tctacttcta aaatatcccc taattttccg ctttcgatta
    10981 ctttttggac tgtcaagaag tcagaatcga accgtctgtt ttggtaacat tggataaata
    11041 gacctttttc ttttgctaat gcaaataact cttctgcttc ctttgacgtt tctgcaaaag
    11101 gtttctctac tagtacattc ttaccatgtt cgagtgccat ttttccatat tcataatgaa
    11161 attgactagg tgtagtgatg acaactaatt ggatctcagg atcatcccaa agatcagaca
    11221 gctgatcagt atattcgatg tcttcgaatg ttttccacac tggttcagat ggggaaaaaa
    11281 tttttttgat attgattttg ttcgtaagtt ttcttgaaaa aggcaaatga tagcgatttg
    11341 tactctttcc attgccaaga taagcgattg taagcatgag tggtgcctcc ttttattgat
    11401 gttttcagta tatcctagaa aagcgcagaa agtatttata atagctattc tggtaaaaaa
    11461 gttcagaaaa acacttgtta tcattcggtt tggtgaaatt aatgttcaaa gtaaggataa
    11521 aaatcaaaaa gatagtagcg gaaccaagtg tttatcagta agatagaatt tggaggtcag
    11581 atcatgctta ttgaagaaaa actaacaaaa caagagttgt ttaccacgac agagaaacgg
    11641 attgtcgatt acatccgtag gaatatcgaa gctgcagtct acatgacgat tgaagaatta
    11701 gcaaaagcta cgtatacttc ccattcagct atcattcgtt tatgcaaaaa aatgggattc
    11761 agcggattta aagaattccg tttcgagctg gggcgggaag ttcatcaatt gattgctcag
    11821 ttcaatcaaa cgattgatcc taattttcct ttttcaagtg aagatagcac tcagcaaatt
    11881 gcaaagaaaa tggcggattt atcgatccag tcaataaaaa aagcacaatt gcagattgaa
    11941 catgaagatt tagatcaaat cggtaaagta ttgaacaagg cacagcgaat ctttcttttt
    12001 gcaaaaggtg attctcaaat cacggcaagg aaattccaga ataaaatggt caagttgaat
    12061 aagtttttga tcatggctga agaatacagt gattcttctt ggaatgcagc caatttgact
    12121 agcgaggatt gcgccatttt tattagctac agcggtcgca tccatcatta tgaacgaata
    12181 atgacctatt tgaagcatgt agaggcccct accttgctga ttaccggaaa tcagcattca
    12241 gaaatggcca aacaggctga tatgtgtctt gttatatcgc aggaggaata tgattttgct
    12301 aaagtagcga ctttctcctc tcaaattgcc ttcgattacg tattaaatac gttattctca
    12361 gtgatatatt ctcaaaattt tgaagagaat atccgcaact tgaaaaacaa acaagaattg
    12421 atgcaaaaag gccttcttaa agaatatcgg aaaacttaag aaaagatcct catttagtaa
    12481 taagaaagat agtgtttgat aggctctctt tgatatcatc ataaaaacag gctgaagttt
    12541 tctttttcaa acctcagcct gtttgtttac ggaacgattc gaatagatat attatagaaa
    12601 tgaggaatag ctaacttctg tttcttattt tgaacgattg acgaaaaaga tatataagaa
    12661 ggaggaaaat cagtatacat gcagctaaca atccatacag gaaaaatcgt ttgttctcgg
    12721 atactttctg tacttctttt tgaacttctt ttgcatgata aggaacccgg tgccctctga
    12781 caagtaagcg atgactgttg accatataag gtgtacaagt taggagtgtc acgaaatcct
    12841 gccctttttc gattctcaga tcttttgtat cagtcggttc taccgtcttg atctgatcga
    12901 cttgataagc gagtgtccgt ccattgattt cgatatagaa agaatcacct aaagtcagtt
    12961 tcggtaagtc tgtgaacaac tttgcttgag gcagtcctcg atggctggaa ataacggcat
    13021 gtgtattttc tccccccgta ggataagacg ttccttccaa taatgaggca cctttttcta
    13081 gcagcacagt attcgtttca tcaaaaattg gcaaatggac attgatctca gggatcgtca
    13141 aaataccgat cgtatgtttc tcaaaatagg ttctatcctt tttcgcaggt tcttttttct
    13201 tagtaaaggg atctgcacct ggatttccac cttcttttgc taattgctgg tttttctttg
    13261 tcatgttttc ttggatttta gccatgactt cttcattttc tttgaccgct tgttgctgat
    13321 aatgagagat gatctgctga tccagataat tattcagcgc gtcgctaaca aatggataag
    13381 ctagtatccc tattcctgag agaagcaaga aaagcatcag cagatctaga agtttccttt
    13441 tctttggttt catttgtgtt ctccttgtga tgactgtttg ataataaaag aaaaaggagc
    13501 atttgcgtaa tccgctgctc ctttttccaa ttcttgcaaa aagactaaat ctgactgtgc
    13561 ttgcgtctag caaagtacag tccagcaatc agtagaagga ctactcctgc accgatatac
    13621 acatagattc ccttaccgcc tgttgaagga agtgtacctt tgtgtttatt tggtattttt
    13681 tcaggagaaa tcaactgtcc tgctgttaca taagattgtt catcgatagt aaatgctaca
    13741 cggtttgtta atggaacata tttttctggc gctttcgttt cttccagata gtacgtgcca
    13801 tattttagac ctgtcacatc gattaaaccg ttacttgtgg ttgtaaaaac cgttgctgat
    13861 tctttcgccg atacccagct gacggctttt gtggatgggt cgatcgataa atatttcgct
    13921 gtgtcactat cttgatcacg aacgacgaat tctgctccag caagtgtttg gtctgatgta
    13981 acgtcaccat ctactttaac aaatcgtttg cccccagtaa cgacatcgac tgacggtggt
    14041 gtttgatcat ttgtatgccc gttatcgaca tttgcttggt tagaaaatcc tttggttgga
    14101 tctgcttttt cgttcaaatg catatagtaa acgaatttca atgtaccgcc aggagttaat
    14161 gaaggaatat aattcggatc aactgaaacc gtgaatccgt ctgtttgctc agtgacagaa
    14221 taatttactg ggtcgatttc tttatttcca tcatataagg cataagcata cgttcctgaa
    14281 gaatcattat caaatgttaa agcagcgtca tgagtatcga taagtttgaa tgttgtgtac
    14341 ttgttttgcg ttccttcttt atcagcgata cctaatggga tattgacaga aatctcatat
    14401 tgaatttttt caccgattgc gacatctttt ccattcaatt gaggtgttgt tttatcaact
    14461 ttagacgtat catttttgat ggtcttttct actggtgttt ggctatttgc caggatctca
    14521 aaaggcgtga ttgtttgctt ttcaatcatt tccgcattat caggagcttt tacttcttct
    14581 aaattatatt ttccaacttc taaatgatta acgatcaact ggcctttttc aatagatgcc
    14641 tcggcaaagt cattgttgcc gatgtcataa gaatgaccag taatgaaatg tttggctttg
    14701 gtttgatcag ttgtccaagt gtacaatcca tctgtgacac tttggatgta ttttttgacg
    14761 cttggtgttc cttcttcttt agaaataata aattctgctc catttagtgc ttcgttttcg
    14821 gcagtaccga tttttgtaac tttcaacgtt ccatcattac cgactgtatt tttagggtag
    14881 agatggatag tatctagttc ttctgtcccg tatttataag agccatctgc ttgtttgatc
    14941 atctcatata cagggaaagc taaaaccatg tttgcggcag ctgacactcc gtcttttggt
    15001 tcttctttga tcgtgtagac tgcatctttc ccattttgtt ttttaggtaa agataaagtg
    15061 acatttccat cagcatctgt cgttcctgaa gcaactggtg taccaggagt caaagactgg
    15121 actgcttgtt ttgctgcatc cacggacgct cctttatctc gttgcgcata aaattcttga
    15181 gtgacgttat aaactgaaaa tgaaatatcg gctaatcctt ggtattgatc gaattcgctc
    15241 atttctttcc cgctgttttg gattaaagga tcgggtaaat cagtcatttt ctttttatga
    15301 aggatgactt gagctgctgt ctcctctgca aacacttttt ttgcgccgaa gctgtttgtg
    15361 agtaatggta aaataaggaa aaggactcct aacataacgt ttattttttt atgatttttc
    15421 attttttcac tcctgttctt tttttgataa acaaccagat tgcaaagctt gcgaatacca
    15481 taccaacaac gcctaaaagt gtatttttcg tttcgtttgt ttttggaaga aatggacggc
    15541 tattttcttt tttattgact actttcaata tactgtccgc ctctttatcc tcagaaagaa
    15601 aagttgaaat ggtaaacgga atttctgttt cacttaaaat ataaccagaa ggtgcttcga
    15661 tctcttttaa tcgataactt cctgttttca acccggaaat tgaaaaagcg ccatttttat
    15721 cggaaatcag gtgaagcgca tctgattcgt tctttgtcca cttgtatccg ttggctgttt
    15781 gctggaggta atttcctgct ttgtttttaa taaggaatac tgcgccagct aatcgtttat
    15841 ctggtttatt tgtctcgatt ttgacaaatt gcttactccc tgtatataca gagactttct
    15901 ttgtactgac accaaaacca aaatccagtg ttccgttgtt gttgatttcc ttgttcgcct
    15961 gtgctgtact gcttagctgc atctgataag aaatggtcag tttttttcca gcatattttt
    16021 ccaacgaagg gatcgaaaat aaaacatcaa atccgtgagg tttcttgtgt atgacaaagc
    16081 cttctgtcag cttttctcca ttcgatgaaa tcgttaaact attaggtaaa aacgttaatg
    16141 cttcatccgc actatctgac aattcgaact tttgatagtc aaggatattt acaggaattg
    16201 tcgtatgcaa ggaataagtg attttttcac caatcgtaaa atcatttcta ggctcttcta
    16261 ataccttctc aaaaggtgga tcagggtagt cgttttcttc atttttagga tacaaatgga
    16321 tagttgaaag tttttgattg ttttgtccat gtacaggaag aacaactacc atattctctg
    16381 ccttttcttt gacgacttct ggtactttgg attcaatgaa aagatacgct ttatctcttt
    16441 tttcttgatc tttagcggcc agagaaaaag aagcgatacc atcttcgttg ttaagagttg
    16501 tagttgttgc ttctgcggta aacataccgg aagacgcacc gttttttgcg atctctgctt
    16561 gtgcttcttc taccgttttg cccttttccc gtagatggta aaaagaatct gtgacatcat
    16621 aaacttggaa tgtgacacca tttaatcctc gatacgtttg taataaagct ttttcttgtc
    16681 cgtcatttgg atgatttttc ggcagttgcc cgtttgggaa aagcaatttg tgtaaagtaa
    16741 tctgaaccat ttctgtttct gtttctgttg ctacctgagt aaaagctggt ttaaatagta
    16801 acaagaagag acacatacta agccaaccaa gttttttcat atttagctgc cccctctttc
    16861 ttttttcaaa aaaagatacc aaatagaaaa cgcacaacca atcatcccta ctagatagat
    16921 tcctaaacgg ccaattcctc ccgtttcagg taatggtact tttgcctgat tcgtgatgtc
    16981 taaagaaatc tggttgtttt tggctcctgg tgacagaaca gattcatgat cttgtccatc
    17041 cacttgaatt gacccatctt cgtgtataac tatagtaact ggctctttta gaccttggta
    17101 tccttctggt gtaaaagttt cggtcagcgt ataagttcca ggttttaaat tttcgaatag
    17161 aaaggtatct gtttcttgtc catcttttgg cgattctaag tccatttctg gtccctgcag
    17221 tctgaatttt gctcctttta atgtctgtcc gttgtcttct tttttgtgta ccgttagatc
    17281 aaacgctttt agtcgatttt gatgagtgag cgtccataca ttatttgtca tctcggtatt
    17341 ttttcccgtg taaatgattt ttccatattt atcgatttgg aattcataat ttcccggcga
    17401 agtatcatat ccggcaggac cagctgtttc aacgactcga tatttaccag gtgtgagtga
    17461 atcaaaactg gcaagacctt tttcattcgt tgtttggctg tcaattggct gataagtacc
    17521 atttgcttct tttttctgta gggaaaatgc tgctccttta agatttatct ctttgtccgt
    17581 cccttgcatc gcatacttac gtactgctac aggaacttca acaaaaggat tttcaatagt
    17641 caactggatc gtatcatcgg aagtagtgac tgtttttcca tcaatggtta ccgtaccatc
    17701 agaagcgatc ttgatttccc aagtagtctt tttgcttaat ccatgccctt ctggagcttt
    17761 tgtttctgtc agcgtatagg tcatttcttt ttgcaatttg acattttctg gaagagaata
    17821 ggttccgtcg ccatgatctg ttagtaaagt atcaatagaa tctcctgtta atttgaaaac
    17881 agcgccaata agatcctttt cacctgtaga ggaatttttc gttattttca agtttaacgg
    17941 tgtgaattgt ttagtatttt tccatgtcat tgcatctatt tgttgagaat cgtatcctgg
    18001 tacaggtaat tctttgattg tttggtaact gaatgcttga ccttgattat tgtattgagg
    18061 tagtgataat atctcttgat aactttctcc gctatttgca gataatttgt caatgtctgc
    18121 acgttcccac gtatttgttg tatctttagc tggtttaatg attcgaatat atccgttttt
    18181 ccaagctgca gcatttgtcg tatgttcccg ttgaatctca aaagtaactt gatctggacg
    18241 atcagctaga ttgttgtcaa attcttccca taactttttg atgtgaagac tgactccggg
    18301 agctttagca gatggtatac cgaattcagc taattcattg gtatcaatgt ttggctggaa
    18361 agttgtcctg ccgttcattt gataccagaa atttggatgg aagtcctcat tttctgtttg
    18421 gattctcact tggtaatgga tttggatttc ttggtctttt cctaaataga tctgattgct
    18481 cttgatggta tttccttcta tggaaataga tggagtgacc gttgtaggac ttgtccccac
    18541 actcttgact gaaagtgtac caggctgata aatgaatggt tctgcgattg gatcatttat
    18601 ttgtccattg cttacagttg ctgagatctg tacagctttt ttggctaggt attcagagat
    18661 atctgttgca tgatcagctg attcatagta aagatcgcct ttttcatctg atgaaaccat
    18721 ttgtcgcata cgagactcta cttctgcttt tgagagacca gctgccggat cgctttgaag
    18781 ttggatgcca agaccatgta tttcgattcc tcgttctttg agtgccatcg cttctccgat
    18841 ggttgcgata aacgtactgt cgattcttct ggatagattg ttttggtcag gtgcatcata
    18901 gattcttgag attagagaag tatttcccgg ccgatcttgc gtattagaaa actgagttcc
    18961 gtaataattg ctgcttgatt gtgcgtgtac ccgctgtact ttataggaaa atgttggtac
    19021 accatccgtc agcaaaacga tcactttttt atgtccattt ggaacggata gcatgcttcc
    19081 tgcatctctt agtgcttttt gtgtaaaagt accaccattt gtccgtgaag gtgtaatgga
    19141 ttttacttga tttttcactg aatcaaatga acccatctgt actgcaccgt tactgtagct
    19201 atatccttcg cttgagtagc cgacatatcc catattgatt ttgtctgtga taccgctatc
    19261 tgctaaagta tcgacaaaac gatcgacacc aatctttact tcaccgatcc gattattgtc
    19321 gttcatactt cctgaccagt ctacgaccaa tacgagatca agaggcgtga tatctttttg
    19381 tacatttcca cgagcattca aataaacatc aaacaatccg ggtgtacttg tttcctttgc
    19441 atactttcgt atcgcatagt ctgcttgatt accggttcct ccatattcga tatacgaatg
    19501 ggtccgatca ttaggatctc cattccaaga atttatacca tcccattgat ttgttccgtt
    19561 tttattgcct tgatgatcaa gaacatttgt atttcctgta ggttgccaac tggctttagg
    19621 ataagtgccc tgatcatctg ttgtatattc tgtaggaatc gtttgattag atggcacact
    19681 cacagctggc aaggataatg taggcaatgt aaatgggctg acagaatttt cgttttcttt
    19741 tgatgattct tcttctattt cgtctggctt tggtactgac acatctgtat ctttcttatt
    19801 ttctgtctca tcaataatgt catattcttt attatcgacg atcagcttgt ttgcctggct
    19861 gtggtaaagt gctggatcga ttggaaaact cagatcgata gtttgtttag tgtttgatga
    19921 gatttccaac gtaattttcc cgctatttac ctgataagca tagccatctc cttcttttct
    19981 ttccacatca cttaagtaat tttcgactag accaatttct ttttctatct tttcagcact
    20041 ggtgttattc gcatttagct tgatgttcag tgcttttttt ccagactgat ccgaaatgac
    20101 ttcagctgaa attgtttcta aaactgttgg atcatctgta gtgtctgcta ttgcagataa
    20161 tggcacaaag tttgataaac tcaatattat cagcatgaaa ataactttca gtttcttccc
    20221 tgtggtcgtc atttttcccc tcctttttct ttttttctgt tttgacgtga atagtgaaaa
    20281 ttattaaaat aaacaggcgt tacataattt tatttatttt aatatgataa ttattatacg
    20341 aagggcgcta aagggaatca caccaatttt cataccaatt ggtttcattt atgaagttga
    20401 ttccttcgat taatgagcgt ttcattgaaa gaaaagggtt gagggagaaa aacatatgat
    20461 caggttgttg gaaggaaact atagaaagat ggaaaaaatc attcgaattc tgtccatttc
    20521 tgacaagtgg atgactaaaa aagaacttgc caaacgaatt ggtagttcag aatctacatt
    20581 tgtaaggtat atcgaagaga tcaaaatccg ctggggaacg gatgtaacga ttcaatcttc
    20641 acaaaaattg gggtatcgtc ttgaacattt gaatgtacca gcttatttaa atgttctgat
    20701 ggaaatgtcg caaagttcta caaacagtcg tttattaaat gagattattg tcaatccggg
    20761 taaaacgatt gagtattatt gtgaaaaaat ttccatcagc cggtcttctt ttacacgtaa
    20821 gttgaaacaa tgcaatcaaa ttttagaaag ctatttatta aaagtgatcg ttgaccaagg
    20881 ttttcagtta actagcctta aaagtgagct ccagttgcgt atctttgtta cctttttctt
    20941 tttaagttac tacggaagac gcacgttgcc atttccaatc gaaaaaaaga agatcaaaga
    21001 aattatggtc aagaataatt gtcagatgaa tttgtttcag gaagaaagcc catatgagca
    21061 atctttcttt attatgtatt ttgttgtgca tctcatgaga gaatcccaag gaactccatt
    21121 tgaatggaaa gctggagact cttatgtaca gttgataaat atcgataaaa aagacttctt
    21181 aatcttgaaa aatctcttag gaaaattgaa gtacagcagc tatataaaag tagtgaacta
    21241 ttttgtcaat acgatttttt cagaattttc ggagaaagaa aaaaatgaaa tcagtgagca
    21301 gatacgaaaa aatatgtcct actgttcgtt ttcatccgct gattgcaaag aaaaaaacga
    21361 agaatatgtt gttcaattat taacgcaaat ttattttttc tcccgtattt tcccatacga
    21421 tacgactcat ttgacgatcc gtgcaactta ctttgcacag cagatgcaac accgccagat
    21481 acgtctatat gctggatttc gtgcagaact tcaattttat tccactctac tgaatcagaa
    21541 tctgatttat tatttatcta gctatatctt ttggatgctt atcggtaatc ctgaaattca
    21601 ccattatact tctgataaaa aaatactgat tatcagcgac ttaagtcttc gacatagtca
    21661 atatattgaa gaatatatca gtgatattct tgctgtgcat aaaattcact ctgagacaac
    21721 tgctattact gaagatcagc tgtcgaaata caatcttctc gagtatgatc tgatcgttac
    21781 gaatcaaccc attttgaatg cccatgttcc tcatatattg attgatgata gtgtgtcatt
    21841 cgcaaatgaa gaagaactca tccgcttatt cgagttatga ataatcaaaa aacagctatg
    21901 tataaaccac cctgataaaa atttcaggac aaatacacat agctgtttct ttttattgta
    21961 ttccttcagc aatccaacga gatttatagc gaaagtcttt taacaactct tcttttgtcc
    22021 aattatttgc aatgagatcc gtcaaagtgt tcattacgag ggtcatggaa aagtatagga
    22081 aactaactgc ttcttccgac gattgaatgg agaaatgatt gattcttaat aattccagcc
    22141 atttttcgtt cattggagaa tcagccgata gttctctacg tttggttgtc tgctgatcat
    22201 ttcgctgggt taacaagtat aacctttgcc aatagatact ttcgtgatct aagtcgcagc
    22261 accactctaa atatttttcg atccccaaaa agaaatgttg tttgttttcg ctaatgaatt
    22321 ttaagatatc ttgatggact atgtgagaac tgcttttgat cgtatacaaa taagcatctt
    22381 ccaaatcttg aaaatattta taaaaagcac cgcgcgacat ccccatcttg tcaacgattt
    22441 ctgagacttt gacttggctg atgtgctgtg agcaaaatat ctccagtaat aggtcatcga
    22501 ttctctgttt ctttgcgttc gataaattga aaaaagtttc ttttggcatc cacatctctc
    22561 ctttatctct gattatacgt ggatagccct tcattgtcat gaaggattca ttccttcttt
    22621 ttctcttctt ctgtcaagta cccatcttcc attcgataaa cgcgatcact ccattgaatc
    22681 atacggacat catgagtaac cataatagtc gctttttgtt tctcatgagc ttctttaact
    22741 agtaatttga cgacttcata tgcatgatct gtatccaaac tagctgttgg ttcgtctgct
    22801 aagatcaagc ttggctcatt atataaagcg cgagcaattg ccactctctg tctttctcca
    22861 ccagaaaggt ctttaggata actattttta agtgactgga tatctaaaga ttgcagcaag
    22921 gaatcgattt ttgttgaatc ttcttttttt gaagtaactt ggtcaactag ttcaaattgt
    22981 tcctttaccg ttaaaaaagg aatcaaatta gagctttgga gaataaatcc tatttcttta
    23041 aagcgtaaat ctgctcgttt tttttctgga agctcagtta gtgagtgacc gttgatcact
    23101 atttctccgg aagtcggtgt ttgcaatcct cctgaaatgg taaggaaagt acttttacct
    23161 gaaccagaag gaccaatgat gctgacgaat tctccttcct ttacataaaa atcaattgct
    23221 ttcaaagctg agacctttgt atgaccagaa ccaaatgttt tggtaacttt tttcatcgtt
    23281 aatatttttt tcatttttat taacctccta ttgctgtaat tgggtctact ttggttatcg
    23341 tacgtatcga aaacaatccg cctagcattg cgacaatgat caaaatccca ctatatagca
    23401 accattgtga ccaaaaaaca gcaaaaggca tcgcgcttgg taaaatcaga ctagtcaaat
    23461 aagcaaacaa aagagcaagc agaacgccta ccaccccaac aataaaggac tgtgctacta
    23521 aagatttagc gatgaagaaa tttttgatcc cttgtgcttt cattactcca aaaatagcag
    23581 tcttctgaag tgtcatcacg tacatgaaaa taccgaccac ggcagcaacc actaagaaca
    23641 aaaagtatat catcgcattt aaggtcatat tttgggcaga atagccagga atactttcaa
    23701 tgaatgtccc aatagttaat aactgtagac ccttcgatgc tttttctttc gaaattttag
    23761 ctgaatcctt tattacgatc ccattgatcg gctgttcttc ttcagaagca aacggctgtt
    23821 gtccatactt caaagcagtc cacgtatcta gggacgcata gatgacggga gaaactgtgt
    23881 aataagtttc aggaaagata ccgacaatcg tcaaaggatc gtcgtaactg ccaattttga
    23941 tcgtatctcc aattttatag cctgaatcag ccagattttg agagatgatg atttcattct
    24001 ttgttttaaa ttcatgccct ttcgtgagtt ttggtaataa aaatgcttct ttcgttgtgc
    24061 cgaaaactgt aacattttct ttgttttttc ctttgattgc tccgctgtac aatccgattg
    24121 gtgctttctc tcctccttca atgtatttca gatctcctct tgttaattgt gaagctgcaa
    24181 atacttgatt tgcatcctcc gacaaaacaa tctcttgtgc gtcccaatca tcgattgctt
    24241 ttttaaactc ttctgaaagt ccattcgcta gtcctgacaa catgaatacg acatacgcaa
    24301 tcaagaacat aataccgacg atcaagccat atcgtaattt agaatagcga atttctttga
    24361 tagctagaaa catttgtttt tcctccctat gatagattga tagtgacaaa atgtcacttg
    24421 ttgaatagtg taactctaaa gtgacaattt gtcacttgtg ttgttttttt cttatcaata
    24481 aattgtctaa gacatctttc gataaaacct taacatgagt cataaaaaat ttatttaagc
    24541 cttatttatg ctttccatga taaactaaag tgaccaaatc aatgaggtgg gaaaatgatt
    24601 aaactattag tagtagaaga cgataaagta ttatccaata atataaaaga aatactcgct
    24661 gcaatcggcg agataacaca agtttatgat ggtagtgaag gactgttcga gttggaaagt
    24721 ggagtttatg atcttgcaat cttggatttg atgatgccgg aaatgaatgg atatgaagtg
    24781 ttagcaaaaa tacggaaaga aaaaatacag actccagtat tgattttgac tgcaaaagat
    24841 agtttggaag ataagattga aggatttgaa cgaggtgcag atgattatct aacaaaacct
    24901 ttctatcgag aagaactgat catgcgcgtg aaagcattat tgaaacgctc gctaggtctc
    24961 tttgatgaaa atgttctgaa gtataaggat atcagttgtg atttaaaaag caatgaagtc
    25021 ttgtataaag aacaagtttt gcccatacaa ggaaaagagt ttgaattgct cgtttatttt
    25081 ttacaaaata agggaatcat tttgacaaaa gagcagatat ttgaccggat ctggggattt
    25141 gattctgata cgacgattac agttattgaa gtttatatga gcaacttacg aaaacatctg
    25201 cgaagtactg gattggataa agaaatcaaa accttacgta atgtcggtta tatactgcaa
    25261 agggtgtgat gcaatgaatg gagatattac acgaaagcaa cgtagtcgct tttttgcgac
    25321 aaatgtagca gcatttgctt ttatcttttt tcttttggga ttcattactt tacagatatt
    25381 aaaccaatct gcatacagag aaacagatct gtcattagag aaaataaatt ttgattctcg
    25441 aacagtacaa ttagagatac aaagatatca gcaaggcgat ccctttttaa acccgactca
    25501 aacggaacaa aacagaacaa acatggaaca gccattaatg gaaccatcaa atgaccgttt
    25561 taacactcaa gtgatcttgt ggtctaaaaa tggtgagata ttgaataaag cagctattgg
    25621 tgggcgtttc aaccaaatca aagaattaaa aatggatacg aaaaatctga ataccatcca
    25681 aaatattgaa ttaaatgaag acagtggctc atctgccctc tctttccgtt cgattacgaa
    25741 aaaagcggag acgaatcaag atggcgttgc ctacatccaa attttagaaa atacaaatca
    25801 aatcaagaat tcattagcga cttttcgcac gattatgatt ttgtgtatga ttgttttttg
    25861 gattctctct attggtatca gttattactt gtctagtcta agcatgcgcc ctattattct
    25921 ttcttggaga agacaaaaag aatttgttga gaatgcttct catgaattgc gcacaccgtt
    25981 aacgattatt caaaatagtt tggaacatct ttttaccaag cctaatcata ctattattga
    26041 agaatccgaa agcatcgctc aagcattgtc agagacgagg cgcctcactg gattgacttc
    26101 ggatttgctg acaatcgctc ggaatgattc tgatcagcag ctgctttcaa agcaaatgat
    26161 caatacacaa gaatatatcg aacaacttgt taaaccattt caagagatgg ccatcataga
    26221 tggaaaagag tttattctcc ataacgatgc aacattagaa gtaccaattg atgaaaaaaa
    26281 aatccatcaa gttttggtta ttttgctgga taatgcatta aaatatacga atccagaaga
    26341 taagatcatt ttggattcat ctgttaacca taaagaatgg ctgattcacg taaagaatac
    26401 tggcccttct atcagtgaag aagatcaagc aaggattttt gatcgatttt atcgggaaga
    26461 tcaatctcgg gcgaaagaga ctggcggata cgggcttggc ttggcaattg caaaacaggt
    26521 aatcgaacag catcatggac agatttctgt atccaattat cttccacaag gagtcgattt
    26581 taaagtccaa ttgccgttga agcctaaaaa ataatgctta tccctaaaaa agggctgtga
    26641 caaaaatctt gtcacagccc ttttttgttg cgaataaacg gtgttccaga agtagcttct
    26701 tcggaaataa gccgaaattt ctttaagctg accacttctg tcacaacctc tttagcttac
    26761 gcttacgatt ttttattgcg gtcttcaatg tgtacatcac cgcttgtgtc aatatccaat
    26821 tcttctcggc taacatcttc tgtcactttt tcaacatctt catgttcttc tttatgaatc
    26881 ccaacttctt cacgaataac tgtatgtttg cttacatcta cttgctcttc tttgattggg
    26941 atagtaatcg tgtcatcttc caatttttcg tctgttccct gtgaagattg atctgttact
    27001 ggtttgcgtt cgatgacgat ttcttcacgt ttgaccggta cttctaccgt ttcagtatcg
    27061 ttgaccacat gtttgtggat gtcgacttct ccagttgtca catcatgtgt atttacatct
    27121 aatttttctt cttttagttg aattgtatca tcatctagat ttggtttgcg atcgatgtct
    27181 tcagtcttgc ttgtacccat tgtatcagtc tcgccaggta cgtccatcgg atcagttcct
    27241 ggtgtcatat tcaatggatc cgttgtattt tgaaagcctg tcgtgccacc tacgtccatt
    27301 ggatctgttc cagtgccagt ttctaatcca gtccccattg tgcttgctgg gctaacagta
    27361 tcttgtgtat ccattgtatc ttctgttgtt tctggctgac gatagccttt tactgtgatg
    27421 acaaattttc catcagaaat atctcctcta tattgatata aaggatcatt ttctggtgta
    27481 gccgtctcag aatcataggt atcaaaagaa aaagtatctt ttattttttc ccataaagaa
    27541 cggtcttctt caccatccat ccgattttca tctgtttcaa catctatacc agacaatccg
    27601 tcaaatccta gtctagatgc tgcttcatca gtagtataaa taacgatatc atctcgttca
    27661 tacccttcgt tacgcaaccg ttcaattact gataatgctt cttccctcgt actatagctt
    27721 ccgatgaccg ttaaattttt ctcttccatg taaattcctc ctagcaaata gatttatgat
    27781 tggtatattg ttactt
//
LOCUS       DAZULO010000026        27657 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000026 DAZULO010000000
VERSION     DAZULO010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.027"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..1349
                     /gene="lysA"
                     /locus_tag="ACG8OA_001142"
     CDS             90..1349
                     /gene="lysA"
                     /locus_tag="ACG8OA_001142"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFS4975968.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1336..2136
                     /gene="dapF"
                     /locus_tag="ACG8OA_001143"
     CDS             1336..2136
                     /gene="dapF"
                     /locus_tag="ACG8OA_001143"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFS4975969.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2139..3020
                     /gene="dapA"
                     /locus_tag="ACG8OA_001144"
     CDS             2139..3020
                     /gene="dapA"
                     /locus_tag="ACG8OA_001144"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFS4975970.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3017..3733
                     /gene="dapB"
                     /locus_tag="ACG8OA_001145"
     CDS             3017..3733
                     /gene="dapB"
                     /locus_tag="ACG8OA_001145"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFS4975971.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3739..4434
                     /gene="dapD"
                     /locus_tag="ACG8OA_001146"
     CDS             3739..4434
                     /gene="dapD"
                     /locus_tag="ACG8OA_001146"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFS4975972.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4450..5451
                     /locus_tag="ACG8OA_001147"
     CDS             4450..5451
                     /locus_tag="ACG8OA_001147"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFS4975973.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5448..6809
                     /locus_tag="ACG8OA_001148"
     CDS             5448..6809
                     /locus_tag="ACG8OA_001148"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFS4975974.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6769..7920
                     /locus_tag="ACG8OA_001149"
     CDS             6769..7920
                     /locus_tag="ACG8OA_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS4975975.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7895..9064
                     /locus_tag="ACG8OA_001150"
     CDS             7895..9064
                     /locus_tag="ACG8OA_001150"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS4975976.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9239..10618
                     /locus_tag="ACG8OA_001151"
     CDS             9239..10618
                     /locus_tag="ACG8OA_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4975977.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10899..11696
                     /locus_tag="ACG8OA_001152"
                     /pseudo
     CDS             10899..11696
                     /locus_tag="ACG8OA_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11885..12553
                     /locus_tag="ACG8OA_001153"
     CDS             11885..12553
                     /locus_tag="ACG8OA_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFS4975978.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(12753..13139)
                     /locus_tag="ACG8OA_001154"
     CDS             complement(12753..13139)
                     /locus_tag="ACG8OA_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975979.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(13111..14493)
                     /locus_tag="ACG8OA_001155"
     CDS             complement(13111..14493)
                     /locus_tag="ACG8OA_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS4975980.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(14649..15836)
                     /locus_tag="ACG8OA_001156"
     CDS             complement(14649..15836)
                     /locus_tag="ACG8OA_001156"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFS4975981.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(15843..16874)
                     /locus_tag="ACG8OA_001157"
     CDS             complement(15843..16874)
                     /locus_tag="ACG8OA_001157"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS4975982.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(16890..18344)
                     /locus_tag="ACG8OA_001158"
     CDS             complement(16890..18344)
                     /locus_tag="ACG8OA_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFS4975983.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            18519..19424
                     /locus_tag="ACG8OA_001159"
     CDS             18519..19424
                     /locus_tag="ACG8OA_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFS4975984.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            19515..20192
                     /locus_tag="ACG8OA_001160"
     CDS             19515..20192
                     /locus_tag="ACG8OA_001160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS4975985.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            20189..20359
                     /locus_tag="ACG8OA_001161"
     CDS             20189..20359
                     /locus_tag="ACG8OA_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975986.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            20473..21369
                     /locus_tag="ACG8OA_001162"
     CDS             20473..21369
                     /locus_tag="ACG8OA_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4975987.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21424..22794)
                     /locus_tag="ACG8OA_001163"
     CDS             complement(21424..22794)
                     /locus_tag="ACG8OA_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4975988.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            23032..23778
                     /locus_tag="ACG8OA_001164"
     CDS             23032..23778
                     /locus_tag="ACG8OA_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS4975989.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            23775..24614
                     /locus_tag="ACG8OA_001165"
     CDS             23775..24614
                     /locus_tag="ACG8OA_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS4975990.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            24615..25565
                     /locus_tag="ACG8OA_001166"
     CDS             24615..25565
                     /locus_tag="ACG8OA_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS4975991.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            25706..26458
                     /locus_tag="ACG8OA_001167"
     CDS             25706..26458
                     /locus_tag="ACG8OA_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975992.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            26477..27628
                     /gene="queG"
                     /locus_tag="ACG8OA_001168"
     CDS             26477..27628
                     /gene="queG"
                     /locus_tag="ACG8OA_001168"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFS4975993.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         9078 a         5041 c         5819 g         7719 t
ORIGIN      
        1 tttgtttaat attattccaa taaaattcaa atctttatca aatgaaattc caataaaaac
       61 ggaaaaaaag tgaaaagagg aatgaaaaaa tgacagaatt gataattggc ggagtcacag
      121 caagcacatt ggcagaaaag tataagacac ctctgtatgt gtatgatgaa gaaaagatga
      181 gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa acgttttgat acaaaagtgc
      241 tttacgcctc taaagcattc caaacgattg agatgctgca tttagttcgt gaatatggat
      301 tcgggctaga tgttgtcagt ggtggggaaa tctataccgc tttgcaagct gattttccta
      361 gagatactat ctacttccat ggtaacaaca agacgccaga ggaactgatc tatgcacttg
      421 aaaatgattt gttgcatatt gttgccgata atttgatgga agttgaatta ttagcagaat
      481 taagccaacg ctatttaaaa aaaatctgta tcatgcttcg gttgaatgtc ggagtagaag
      541 cacatactca tgaatatatc gtcactgcgc atattgattc aaaattcggc atgagctatg
      601 aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga aagcagctat ctagagttag
      661 aaggatttca tgctcatatc gggtcacaaa tttttgatgt tactgcttgg ttagctgaaa
      721 tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc tctttccttg aatcttggcg
      781 gagggtttgg gattcgttat acacaaaatg atcagccgat cccagtcgaa gaggcactaa
      841 aaaaactgat tgaatatacg gaagaagctt tggaaaaacg aaaactgacc attcgtcaat
      901 tactgatcga accgggtaga agtattgttg gagaagctgg tacaacacta tacacgattg
      961 gtttcatcaa aaagacacca cataaaaaat attactttgt agatggcggg atgacagata
     1021 atatccgtcc agctctgtat caggcagaat acgattgtga tttagcagca aaactgtcgg
     1081 aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg tgaatcaggc gatgtggtaa
     1141 ttaaagaagc ttatttgccg aaagcagaat cgggtgatct tttagctgtt tattccacgg
     1201 gagcttatgg atactcgatg agcagcaact acaatcgagc gacacgacca gcagttgttt
     1261 ttgtcaataa tggccaatca agactgattg ttcgcagaca agcattttct gatctactaa
     1321 ggggggaagt ttcctatgat ccttcataaa tataatggct gcggaaatga ttttatctta
     1381 ttagattacg cagaagacac agattatagt caattagccg cttctttatg cacaaaagaa
     1441 aaatatgata cagacggatt gatcgccgta aaaatcgatc cgttggaaat gatctattac
     1501 aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca ttcgctgttt tgctcgatac
     1561 gtaaatgatc agaacatgat gaatgaacag gaattcaacg tagagacatt agcaggcaca
     1621 agaaaaatta cggtgttgga tccctcacct ttttattgca gcgtagatat gggagtgcca
     1681 gatgattctc cggaaaaaat caaagctaat caaacttatc caataaaaaa ccaggaaata
     1741 gagatcaatg gtgaaaaagt aacgatcact agtttatttt taggaacgat tcatacagtt
     1801 gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa gaggtgaact gatctgtcat
     1861 catcctttgt tcaaagaaaa aaccaatgtc aactttgtag aagtagtcaa tgaacaagaa
     1921 ttgatcgtcc gtacctatga aagaggagta ggttggacac ttgcttgcgg tacaggttgc
     1981 tgtgcctctt atgtggttgc taaggaaact gggaaaattt cagcagatca ggtagttgtt
     2041 cacttggaac aaggagactt gatgatctct ggtgaggatg cgatacaaat ggctggccca
     2101 gcagttcatg aatggacaag aatattggag gaatgaagat gataaaaggt tcgatcgtgg
     2161 cgctgatcac gccgatgaat gaagagggat cagtagatta tgctggtttg gaaaaattga
     2221 tccaattcca tttagatgaa caaacagatg gattattagt tttaggtaca acaggagaat
     2281 cttctacgct cacacaatct gaggaggagc agatccttca actgacagta aagaaagtgg
     2341 ctggccgtgt gccggtgatt gcaggagctg gaacgaataa tacgaaagaa acgattgaaa
     2401 aagcgaagca ttttgcttct ttgggggcag acgcactttt ggtcatcacc ccttactaca
     2461 acaaaacaag tgatgcagga ctagctgctc attttacagc aattgcagaa gcctcaccga
     2521 ttccgcttat tttgtataat gttccttcta gaacaggcat gtcgattcca atccatgtat
     2581 tagtaaattt agcagaacat cctaatatca ttggtttgaa agaagcctca ggagatatgg
     2641 cttatgtgat ggatgccgct cggttgattg gagaagaatt cttcctttac agtggaaatg
     2701 atgatttgat cctaccagta atgagtgtag gaggaagcgg tgtaataagt gtatgggcga
     2761 atatccaacc taaaatagtc catgaattag taaaagatac gcaggacgga agatggcagc
     2821 aagcaaaaga aaagcaatta aatgcacttg aactgatcca tgccttgttc agtgagacca
     2881 atcctattcc tgtcaaagca gccatgtcat tattagactt accttccgga cctctacgcc
     2941 tgcctttagt ttctctttca aaagagaaaa agaaacaatt agctcagctt ttgttaaaag
     3001 aaaggacggt tgaaaaatga aaatcggact gattggcagt ggacagatgg gaaatagagt
     3061 cgaagaagtt gcacaagaaa gaggagatac ggtccttctt ttacggacgg cacctaaaga
     3121 agcaactacg cttgctttta cggaactgcc agaactgctt attgattttt ctcatccaga
     3181 taatttagag aaaatccttt cgtatagctt atcatatcaa cttccgttag tcattggcac
     3241 gacaggttat acacaatcac agttccaaga aataaaagaa catgccaaac atgtacctgt
     3301 gatgtatagt gctaattttt cgttcggcat catggtgatg aatcaactaa tcaagcaagc
     3361 agctgcaatg ttgcaaggct ggcagatcga actgattgaa aaacatcata gtcgtaaaaa
     3421 agatgcacca tctggtactg caaatatgct gcttcaaaca atccaagaag tgcaaaagct
     3481 gcagcccgta tacaactggc aagggaaaaa aagagaagaa aaccaaattg gtgtacacag
     3541 tattcgtgca ggttctttac ctggtgaaca tgatgtattg tttgctgcta ctgatgaaat
     3601 atttacgatc aaacatgaat ctttttctaa tcgaatcttt gctgcaggcg cagtagaagc
     3661 agcggactgg ttgaaagatc agtcaccagg ttattacaaa ttagaagatt tattaatgga
     3721 caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc gctacattca aacggcatct
     3781 aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc caatcgaatt taaagaaagt
     3841 aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg actatgagac gattgctccg
     3901 gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa tcgaaactgc tgcaagatac
     3961 agcgcagttc ctttgctaga tattaaaaaa attaatgctc gtatcgaacc aggtgctatt
     4021 atcagagatc aagtgtcaat cggcaacaat gccgtgatca tgatgggagc gattatcaat
     4081 attggtgcag taatcggtga aaacacgatg attgatatgg gtgctgtctt aggtggacga
     4141 gcgacagtgg gcaaaaactg ccatattggt gcaggagctg tcttagcagg tgtgatcgaa
     4201 ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac tggtaggtgc gaatgcagtg
     4261 attgttgaag gtgtccatat cggaaaagat gcagtcgtgg cagcaggagc agtggtttta
     4321 gaagatgtgg cagcagaaac tgttgtcggt ggaatacctg cacgtgtatt aaaaataagt
     4381 gatgatcaga caaaagaaaa tacagcattg attgctgcgt tacgcgaatt atagaggaga
     4441 tggagaaaaa tgcgcgaaat agatacctgt attgtggggg caaccggatt agtcggccaa
     4501 acgatgctca aagtgttaga agaatatgat tttccagtag gacgattgaa attattagct
     4561 tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa agaaacacac tgtacaggaa
     4621 ttgacagaaa cttcatttga aggatatgat ttagcgctat tttctgcagg tgcttctgta
     4681 tccaaagagt ttgcgccaat cgcacgaagt cagggactat ttgtcataga taattcctca
     4741 gcatggcggg aagattcgga tattccatta atcgttccag aggtcaatct ggaagactat
     4801 acattgaatc ggctgattgc aaatccaaat tgttcaacca ttcaagcggt acttccgctt
     4861 aaagctttac aagaagcctt tggtatccag cgagtcaatt attcgaccta tcaagcagtt
     4921 tctggttccg ggcaaaaagg cattgaagat cttcatcgta cgcaaaatga cgaggaacca
     4981 gttttgtatc cttataatat tagccaaacg gtgattccgg aaatcgatca gcctttggaa
     5041 aatgggtata caaaagaaga acagaaaatg ataaatgaga cccgtaagat tctgcatcag
     5101 cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa tcgaaaatgg acatggagtc
     5161 tctgtggcag ttcaactgca aaaagagttt acgacggaag aaattagaga gtgtctcagc
     5221 cagtttccag ggattcaatt agaagatgat ctagcagcac ataaatatcc gacgagcatt
     5281 cttgctagag gaacagatat ggtttatgtt gggaggatca gaaaagacac ttctttagag
     5341 aacggattgt tgttctatac gactgccgat aacatacgca aaggagccgc tgcgaatgcc
     5401 gtacagattg ctgcagcttt aatgcagaaa atagcggagg tggcaagatg agtaatacgc
     5461 tgaaagtagc gaaattcggc ggaacatcga tgtctcatga agagcaattc aaaaaagtcc
     5521 gtacaattgt tctttcagat cccaatcgcc aagtgatcgt tgtttctgct ttaggcaaac
     5581 gtacgtcaga tgatgacaaa gtaacagact tactttattt gatttatgct taccttcagc
     5641 atcatgttga ttggacaccc ctttggaaaa ggatttgtga tcgtttcatt caagtaagag
     5701 atgttcttgc cctgcagtta cctattgaag atgaactagc acgcatacag aaacaactgg
     5761 aagacgaaaa gataactgaa gattatttag taagtagagg tgaatatctg actgctcaac
     5821 tgatggctga gtatcttggt taccagttta ttgacgcaaa agacctgatt ttctttgact
     5881 atgatgggaa aatcgatcag gaaaaaacag cacaggaaat cacaaaagca tttaaaagat
     5941 atccgaaaat ggttgttcca gggttttacg gggcgaaccc tgcaggaaaa gaaaaattat
     6001 tgggccgtgg gggaagcgac attaccggcg cgatactggc gaagatgttg caagcggaaa
     6061 gttatgagaa ttggacggat gtttcaggaa tcatgatggc tgatccgcga atcatcgatc
     6121 atcctaaaat catcgaggaa atctctttta gagaactgag agagatggct tatatggggg
     6181 ctaacgttct tcatgaagca gctgttttcc cagttcaaga ggctgatatc cccatccaaa
     6241 taaaaaatac caatgatcct ttcgctcaag ggacaaaaat atcgaatcac gaaatagaaa
     6301 aggaaaatgg actgacagga atagctggac gcaaggattt tctttccatc actttattca
     6361 aacgtcatat gtccgatgaa atcgggttta tctggaaagc aatgagcatt tttgctaaac
     6421 atgagatatc aatcgaacat attccttcag ggatcgacaa tatcggagtg atcgtctcag
     6481 cagaagcgat tgccgataag ttatttctca tcacgaaaga attaaaagaa acacttggcg
     6541 tggaagaaat cgtcgtgatc gaagacttag ctttgatctc tgtcgttggg ggtccgcata
     6601 aagaattgat tggattatct gggaaagtgt tatcgatatt gaacaaatta gaaattcgca
     6661 catcgatctt atcacaagga gcacaggagt tgaatctgat catcggtgta ccgaataatc
     6721 aatatgaaac agtagtgaag ggtatctatg aagggatggt gaatacggat gctgaaagaa
     6781 tatcgaatgg cactccatca aattcctgaa atcgggtttc atgaattcaa gacaaaaaaa
     6841 tatttatatg aacaagtaaa aaacagcggg gccgttatcc atgaaatcgg cgaaacagga
     6901 ctactccttt attttgataa tcagcaagaa gaaacgattg cttttcgaac agatatcgat
     6961 gcactgccag tcaaagaaga aacgaatctc tcttttgctt ctaagcatga aggttatatg
     7021 catgcatgtg gtcatgacgg acatatggcg atgcttttag ggttgactga ctttataact
     7081 aaacacattg aggatcttat ttataatgta gtactgatct atcagccatc agaagaaatt
     7141 tccggtggcg cacaaagcgt tattcagtca ggattgttgg ataaatatga agtacaagca
     7201 atatttggct ttcatctatg gcctggacta gctaagggag aagttttctc tcgaccagga
     7261 cctttgatgg cacaaagcag tgaaacagac attatcatta aaggccagtc tgcacatatt
     7321 gcttctagtg aaaaaggaat cgatagtctg gaggcagctg tacgattttt gaagaatgtt
     7381 tatgattttg accggcaatt accggcagag tggaatcatc taatgaaatt tggccaaatc
     7441 caaggtggaa cgatccgcaa tgttttagct ggagaagtta ttgtatccgg aagtatccgt
     7501 tcttacagtt cagatatgca aaattatttg aagaggggat tagaccattt agcagaacag
     7561 tttcactcta cagctactat ttcttttcgc tataatgacg gctatccagc tgtaatcaac
     7621 catagaggct tatatgcagc actgttagat tcaaacaaac tacatgaatt agatgctcca
     7681 gttcttcaag ccgaagattt tggcatatat acgcaacaat atccatgtgt atttttcttt
     7741 ttaggtgtcg gtgatacccc tgctttacat gatccgacgt tcgattttga tatggatgtt
     7801 ttagaaaatg gtgtagaatg gtacaaaacg attcttttta cagaaaatct tcttgtaaaa
     7861 atcgatagca gttcaaaaga ggagcgagta aataatgatg aatttatcca atcgatttag
     7921 tcctaggctt tctagaatag aagtatcaaa aatacgacaa ttcgatcagc aaatatcgtc
     7981 gataccagat gtgatcaaac tcaccctggg agaaccggat tttccaactc ctgaacatgt
     8041 caagcaagca ggaatagcag cgattgaaga agacttttct cactataccg gaatgagagg
     8101 attagaggaa ctgcgggaag cggcttgtat atttcagcaa cagcgttatg gattaactta
     8161 tgatccgcaa acggaagttt tgacgacagt tggtgcaacg gaagcgattg cctctgcgtt
     8221 gttatctgtt ttggaagaag gagacaaggt tcttattcca gcgcctgctt attcaggata
     8281 tcagccatta gtggaactag ctggtgctga attgatcccg atagatactt ctgacacagg
     8341 gtttgtctgt cagccggaac aatttgaacg agcatttgaa caatatggat cagctgtcaa
     8401 agcagtcatt ttaaactatc ctaacaatcc tactgggacg acattgtctg caaaacaatt
     8461 gaaagcaatc gctgaggttt taaaaaaata tccggtattt gttataagtg atgaagtata
     8521 tgctgaattg acttatagtg gaacgcatat gtccatagct acttatcttc cagaacaaac
     8581 aatcgtgata agcgggttat ccaagtcaca tgcgatgact ggttggcgag tgggctttat
     8641 ttttgctcaa aaagccctta ttgacgaact aattaaagtc catcaatatc tggtgactgc
     8701 cgctacaacg atgtcgcaaa aagctgcggc tgaagcgcta ataaatgggg tgaaagatag
     8761 cgagaatatg aaagagcaat atcaaacacg aagagactat ctggtccagc aattggcgcc
     8821 tttaggattt gaagtgaccc agccaaacgg tgcattttac ttgttttgca aactcccagc
     8881 tgccattcaa actgactctt ggcagttttg tgtagaatta gcggaacagg caaaagttgc
     8941 gtgtatccca ggaattgcat ttggcccaga aggagaaggt tatatccgga tttcttatgc
     9001 tagcagcatg gaaaaattac atgaggcctg caaacggatc aaagactttt taacagaaca
     9061 gtgattcata gattttatca aaaaaacaat cgttaatttt gcggtatatt ctgcaaggag
     9121 acgattgttt tttaaattta gttcaaatga acgcgtttct tttgattcat aactaatgta
     9181 atgttaagtt taaattgttt agaatgattt taaaagaata ttcaaggagg tttcatcaat
     9241 ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga gggcatcaat cgatcttgga
     9301 actattgaca cgatacggtg aaaatgtaga tatcacgcta tttgaagcag gagactatat
     9361 ttccttcatg tcatgtggta tggagctgta tttagaagac aaggtaacag atgtaaatga
     9421 tgtacgtaat ttcagaccag aaaactttcc gcaatccaat gttcatattt taaataatca
     9481 cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg gttactagcg catcagataa
     9541 cgaaacaaaa gaatatcctt atgacaaatt gattttgagt tctggtgtca aaccaaacaa
     9601 tctgccagta ccaggagcag atctagaaaa tgtctacttg atgcgtggat acaattgggc
     9661 aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa aaaatcgctg tcgtaggtgc
     9721 gggttacatt gggatcgaag cagcagaagc aagccgaaaa gctgggaaag aagtcgtatt
     9781 gcttgatgtg atcgatagac ctttaggcac ttatttagat gctgagatga cagatatttt
     9841 agaacaacac ctaaatgaaa acggtattga agttttgaca aatgcaaaaa tcgaagcttt
     9901 tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa aaagaaatcc cagcagatct
     9961 agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg ttaaaaggga tcgtcgactt
    10021 agatgaacgt ggctggatcc aaacaaatga atacttgcaa acgaatcttc cagatgtgta
    10081 tgcagtagga gatgctacac ttgcttattc tatcccagca cgcaaaaaaa tgccgatcgc
    10141 tttagcgact gttgcacgta gagaagcacg ttatgtagta aaacatcttt ttgaagaagt
    10201 accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca ttaagtgtct ttgactatca
    10261 ttttgcagca agcggattga atagctttac agcagcaaaa gcaggagtag atgtacgagt
    10321 gtcttattat gaagatacgt tacgaccaaa atatgtacca gaagaacaag gaaatccaaa
    10381 agtgtccatc caattattct cggatccaat gacccatcaa ttgctaggag gagcagtatt
    10441 atctacctat gatgttacag gtcaaggaaa tgtattggca ctagcgatcc aaaacaaact
    10501 gactttagaa gaactagcag aagctgactt cttcttccag ccaggctttg accgacaatg
    10561 gagcatgttg aatctagccg ctcaacaagc gttaggagaa gaaccatttg tagaataaga
    10621 aaacgaaata tttgaaaaga ggttgtgaaa aagctgcctt gcatcaagta ataagaacag
    10681 ccaagtaaaa acagcttctt atgtttttaa cttggctgga cttattaccg cagatgcaag
    10741 cttttgaaca ccgtttattt tcaaaaaagg gactgtgaca cgatttgtgt accgaccccc
    10801 aaaagttaga ccaaaaaatc taacttttgg aggtcggtat tttttatggt taaatatact
    10861 tttgaactta aattaaagat cgttcatgat tatttggatg gcaaaggcga ttggaccgta
    10921 aaaatccaga tcaagagatt gaagcggaaa tgcttaaaat tcgggagaag cacaaggatt
    10981 atggctgttt acggatgacc aatgaactaa gaaaccgcgg tttctctatc aataaaaaga
    11041 aagttcagcg cttgatcaag aagctaggaa taaaagtaac ggcatacacg cgtaaatcac
    11101 gtcgctacaa ttcttatcga ggaaaagttg gagcagtagc caaaaatcgg atccatcgtc
    11161 gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa tttaaatacc
    11221 atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat ccttttatgg
    11281 atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat gcattagcgg
    11341 taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat cgtcgtacgt
    11401 ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa ttgaaggagt
    11461 acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca cttatggaga
    11521 atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac tgtttcgagg
    11581 aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg ataaaacaga
    11641 aattaaactg gcaaagtctc gtccaatttc gtaaaacaac cgtaacggtt gtgtaaaaat
    11701 atcattttag agcataaaaa aaaggagtgg atttctccac tccaaaaaag tctaactttt
    11761 gggggtcact acagtgtcac agtccttttt ttagtaaaag gatacatttc ttccttgtat
    11821 attccatgcg tgttcgtgag aaagcatata taatttaatt aacagatagt tagggggaga
    11881 aaaaatgatt ttttcaatta caatgaaact ggtgttcggg ttgatcggtt tattattagt
    11941 ggtacgtttg cttgggaaga aatccatgtc agaaatcaca ccgtttgatt tagtctatac
    12001 gcttgtttta ggtgggatct tagaagaatc aacctatgat gacaatgtcc atgtagggca
    12061 tgtcctattt gccattgcgc tttgggcagt aatgatttat ggtatcgaac gtatcgtcca
    12121 aaaaaatgaa aaggtcaatc gttgggtcaa aggcgagcca tctgttctaa tcaaagacgg
    12181 cgtcatcaac atgacagagt tgacaaataa ccatatcgag atggagcagt tgcgagcaat
    12241 cttaagacag caagaatgct tttctcttga aaatgccaaa catgttatcc tagagaatgc
    12301 tggtcaaatg agtgtattga aaaaatcaga tgaagacaaa gctttatcga tcctgcttgt
    12361 agatgaaggg caaattcagc ataaagtgct tcagtccaat caattgactg aagcatggct
    12421 tatggagaac ttgaagaaag aaggctatgc agatgtaaaa cagctgattt atgttgaatg
    12481 gtcagaagaa aaagggctct acatactaaa taaagaagac gtcatgcatg aatcttaccg
    12541 aatagatgga tagatagaca gaggttgtga aatcagcgcc ttggcttgtt actcaaaact
    12601 aagcgacttt ttcacaacct ctgatatcta atatacgttg ttccagaagt tgcttcttcg
    12661 aaaaataagc caaaattcca caaaaattta agaagcaatt ttcgaaaaat cctttcttat
    12721 tttttgaagt tgatcacttc tgtcacaacc tctcatgtca caaaccattt ttttgtttcc
    12781 tttaactcaa tataccgtac gtcttcccac aatatcggct gatggtcaat cataaagtaa
    12841 tcttcatagg cttcaccaat gaagtgccct tcgacctcat ccaagagtct tccaaattca
    12901 tcttttaaat tgagctgaat gattactgct ttacgagata aaaaggcttc tgtcagtata
    12961 ctatcgattt cttgcaatgt catttgttgt ttaggaagag gctgtttgcg tgcttcttct
    13021 tcattttttt tgataccgtt gactagttct tccatcgcaa aagcagtccc ccattttaat
    13081 ccaaatggcc gatctcgata ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc
    13141 ttttccacct tctaatccac tcataccacc tgcatgcccg ccaacgagag aactacgtgc
    13201 gatggcgcgt cctccttgca agagactatg cgcatggatg attgattgaa agccgtattt
    13261 ttttctaatc gtatcgacaa caaaatcaac tttcgcttca tttatctgct tttcaggatc
    13321 ttcaaaaagg ttaagctgta aacctgtcgc gtatacgagg tcggaacagt tcacgcctac
    13381 acgccgaata tcctggtagt ggtaatgctg gtggaaaatg gtcaataaag catctgctag
    13441 atctctgcta gcatttgtcg cttcgatttt catttgctgg tgaaacccgg tcttgccatt
    13501 tggatcaaga tagcctaaag agtatcctac ccataagctg actacttgtg tttttgctgc
    13561 agatcgtctc aagcgagtgc cgacttggtc ggccatttct ttgataacga tctcgatttc
    13621 ttctcgtctt gtataatctc tatctaggat ttgactattt ccgatacttt ttgattttgg
    13681 tgtatattct tgtcccaaaa aacttcgatc gatgccccaa gcatgggcat aaagctgagt
    13741 accgatgacc cctagcttgc ttttcaagtg ataataatcc gcgtgagcta aatcataaac
    13801 agaatgtata cccatttgtt tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc
    13861 agtgatatct gagattttcc ataacttttc tggtacatct tcataacgcc attcagcaaa
    13921 catttgcttt ccgtcttttg agccattgtc taaggctagt ttagcaagta gcgggttatc
    13981 gccaattcct atagtcgtgt agattccggt ctgctcaaac acatctttat ggatcagttt
    14041 agccatgtcg tacgcatttg ttttaccaaa gagtgccatg ctatttgtta catcaaggaa
    14101 gctttcatcg acggaataga catgatggtt cgcttcgtct gcatattttt tatagataga
    14161 attgatttct ctgttctttt ccatataata ggccatcctt ggggcaacaa tatataaatc
    14221 acttggatag gggaagggaa gatctctggc acggctgata ttggtgatgc cgtaagcttt
    14281 ttttgctgca ggactggaag ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga
    14341 cggataggac atgactacta atttagttgt aaggggatca agatcctttt caacgcactc
    14401 tacggaggcg taaaaagatt tgcagtcaat acataaaata tctcttgaag gttctttttc
    14461 ataaacaaat accggatttt gattcgccca cataacaatc acctcttcaa cacaattaga
    14521 acacacgttc tctttccgtg tcaataaaaa acagcaattt tcaaatacga gcaaaaggct
    14581 cacatttgga aattgctgag aggttatttt tatctatttg tttttagctg agatcgttga
    14641 tttttttatc aaacatgaac agcttctgga tcgatctttg ttgtcccatt tccaatgtgt
    14701 gcaacataaa attccggagc atagccgaca actttcatgt attcatcata gacattgttt
    14761 ttgaaggcag gtatattttc ttctttgaca agagcaatcg cacagccacc aaatccagct
    14821 cctgtcattc gtgcacctaa gacaccttct tgtttttgag cagcatctac tagtgtatct
    14881 aattcgatcc cagttacttc ataatcatag cgaagagagt ggtgagaatc atttaacagt
    14941 tgaccaaatg cttctaagtt cccagcttct aattcagctt ttgccttcaa agtccgctga
    15001 ttttcagaaa cggcatggcg agctctgcgt atcaatgtat catcgccgat ttggtcaata
    15061 gttgattcaa attcttcttc atctaagtct cctaaagaag agataggcaa agttttttgc
    15121 aaacgtgcca aggcttcttc gcattcgcta cggcgttcat tgtattttga atctgccaat
    15181 tcccgccgct tattcgtatt catgatgacg attgcataac catctagttt cactggaacc
    15241 atttcgtatt tcagcgtatt tgtatctaat aagattgcat gatctagctc accgaagccg
    15301 atggcaaact gatccatgat acctgaattg acaccgatga attcattttc tactttttta
    15361 cctgtttgga ctagttccaa gcgatctgtt tttaaatcaa acagatcttc taatacaaca
    15421 cctactaata attctaatga agcgctactg gaaagtcctg caccattagg gattgttcct
    15481 tccactaaca actcaaaccc atgatcgatt gagtgtccag cttcttttaa tttaaggacc
    15541 attcctttta cgtaatttgc ccagtttgct tttttatcat attctagctc atctaaagta
    15601 aaactaatga caccgatatc gtcaaagttt gtggaataaa ctaatatttt attatcgtct
    15661 cgaggtgctg ctacaccttg cgttccataa gtgatggaag cggggaagac atggcctcca
    15721 ttgtaatctg tgtgttcacc aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt
    15781 tttttaggtc cgaatgtttc ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg
    15841 agttattcct ttcttgtttt gattttgaat tctgtcactg tttcaaatac tgatccagct
    15901 tttaaatgaa tcgaaccaaa tgctgagaag cgctctgcac ctggagctgt ttgagtctca
    15961 aatgtgatgc caccgtgatg tgccagtttt cggttgcgca tttctggtgt ttctgtgccg
    16021 aaatttgcag tgaaaataac cacactagaa gcatctgtag caatacctac ttggattctt
    16081 ttatcggggc tagtcaagcg agcggcttct ttacccaagc cggtttcttt taagaaaaat
    16141 ggatgatcga ttccgtcaac aagatttttt tgatccaaat cactagcaaa aacggacgct
    16201 aattttttag gtatttgaaa atcgaaagca cttcctgcag cattttcttt tacccctata
    16261 ggaatactat ctgttcgcaa aggagcataa gcttcgctat tgagccaaag ctcatgttga
    16321 tcgattgact gagaagcatc tcccgtcaag ttgaagtaaa catgatttgt cggattgaat
    16381 aaagtatctt ggtcactggt gccgcgagag gtcactcgcc agatattatc ttttgtcaaa
    16441 gtatagcgga tttccacatc cattgtacct ggaaaaccat gttctccgtc gggacttgta
    16501 gtagtaaaaa tgacagaagc ttcattttcg ccattcaaaa tgacataaga ccattttttt
    16561 aattcaaatc caggagcacc accatgcaga ttatgtccag tttttggatc tgtggctaat
    16621 tggtaagttt ttccattgag agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg
    16681 atagaagcac cgatatacgg atctttttcg atatattctt cagcggtgtc aaatcccaaa
    16741 actaattctc gatcatttga ttttaaactg acgattctag cacctaaatc agagatagaa
    16801 agtgctagct tgtttttgtt ctccaaggtt atcaagtgat aaccttggcc aaaatctttt
    16861 tctgtaatct tcattttcaa gatcctttct taatgggaag gaactactgc atcgtgaagt
    16921 tcatcttcca ttgtatcagc tggagccaat ttgttttcca attcctcaac gatttgtgca
    16981 tgttttttct ctgtcaatgt aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa
    17041 gcagcaggag catagaatgc ataagctttg aaagtcgtta cgccatgagc agtgatatca
    17101 gcagcagtag cattaccagt catcccagaa gcgatagcga caaaccctac gatcccgtta
    17161 gacaaagcac cagcgatttt atctagtaaa gggcgaattg atagagtaac agcttcgtta
    17221 cggataccat ttttccattg accatattca acagagtctg tgatcgtcat caaagcggat
    17281 aagaaaatca attgttgagg gaagtagaac aatactagcc cgatgataac gattggcaaa
    17341 cttgaacctg cgatcgtaaa gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg
    17401 taaacgtagc gacgagagat gacgcgtgta agcattggga atagtggaac agctactaaa
    17461 ccaagtaagg cagcaactag accaacgatc caaaattcag ttggttggcc aagtacatat
    17521 ttgaagaagt agaataatac ggcagttgtt gctacattgg caatcgcata agcgatataa
    17581 gcaagggcaa gccacatcaa ctgatcattt tgagcaactg ctttgaacac atctttgatc
    17641 ccagcttttt cattttgctg acggatcgca gtatcgactt cttttgtgcc caaggcagtg
    17701 ataatcgcag taccgcctga aataacagca gcaatcacac cgaaccagaa ccagcctgaa
    17761 gcgccttcaa tatgtttatg agtaacaaga taggtaaaga aagtaacgat agggacaacg
    17821 actagagtag taccatttgc acctagagaa gagccgaaac gagcaaacgt agcaaatttt
    17881 tcacgttcct ttgtttccgt actcaaagct ggaatcattg accaaaaagc gatatcctta
    17941 aatgaataaa aactatccaa taagatgaaa acgattgcaa ataatatgat aaataaagtc
    18001 gtattatgag tagctaagcc aaagaaattg gtaaagattg ctaccaagag tacggaacta
    18061 acaacgccgc cgataactaa ccatggtttg aatttaccgt attttgtacg agtattatcg
    18121 atgatgccac caatcaaagg atcaaaaata atttcaacta agcggatacc tacaactaag
    18181 cttgtaactg tagcaatcat ttttgcattc gttgccttat ctgttcctgc gaacatctgg
    18241 cttgtcacga ataccataaa ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa
    18301 gcgcctaaag cgtaggacac acgttggatc attttgcttt tcataatctg tattcctctc
    18361 ctgttcaata tataatacta agtaaaccgg ttacaacgaa taacaaagat cttttaatga
    18421 cttaaactta acaagaaatc gcttcctttt taatatattt ctgtgacttt tatttaactt
    18481 tttgttgcat tatactaaag gagtaaggag ggatagacgt ggagcaatcg atttttttaa
    18541 aaaaggaaca tcaacaagta gaaaaagaat tgtattttga tttttgtggt ttctctaaaa
    18601 cgatgccgtt tcattctttt ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga
    18661 tggaaggcaa aggatcttat catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc
    18721 tgttcttgat tcgtccagga gactcgacgt tttacttatc agatggagaa gatccgtgga
    18781 tgtattgctg gctttcattt ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt
    18841 tcagagaaga ccaatacacg atggtctctt ctgacataca aaaatatatc gacattattt
    18901 tagaatgcat gaaagatacg gaagaaacct tggaaaatga attacagtta acagcattaa
    18961 cctaccgttt tttatcagtc cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa
    19021 aggttcattc ctctttagct gttgaaacag tcaaatacat cgaggcacat tatgctgaaa
    19081 aattgtctgt tgaggaaatc gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag
    19141 tattcaaaaa tcatatgggt accagtatca aggaatattt gatcggggta cggatcaatc
    19201 gaggggcgtt tcttctttct ctgacggatg aatccgtgga aagtattgcc tatcaagtag
    19261 ggttcaatag tctagtcgta tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta
    19321 cgaactatcg taaacgtatg aaaaacgaaa attttaagaa tattgctttg gataccttga
    19381 aaaaacagtt ggaaaaacaa accgcagttt cttggacaac ctgattagtc tggttaaaat
    19441 gacaagcatc ggcaaaagaa acttgtggcg gttatgctac actgtaaagc gaagactaaa
    19501 aaggaggaaa agggatgcgt acacttatca tcattagcca tccaacgatc gaagaatctt
    19561 taaatcagca atttttcaaa gaagcttctg ctggtttacc agctacttgg catcatttgg
    19621 aatcaagtta tccagacgga aaaatagata tagagactga actacagctc ttggcccagc
    19681 atgatcggat cattttccag tttccgtttt actggtatag ttctcctgct catttaaaaa
    19741 tttggcaaga taatgtcttg gaacgagcag cacatgtatt aaaagggaaa gaactaggaa
    19801 tcgtattgac aacaggtgtg gcggaaaaag aataccaagc tggaggaaaa gaagaatata
    19861 cgatttccga atttttgagg ccgtatcagc gaatcgcaaa taaattccat atgacctatc
    19921 ttccgccatt cgttttagca caatttatgt atttatccca ggaaaaacgt tgggagaagc
    19981 taattgctta tcagcagtat ctctcattgg aaggcaaacc atcgctgact cagcggattg
    20041 actggtttat tcaaagagta caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac
    20101 aagcatatat cgtagaagca ctgactgagg caaaagagca gatcgaagac ttgaccttta
    20161 cactgcaaga aatgaaagga acttcattat gacaaatgat tggctgatcc aacaattaga
    20221 tgaattaagc gaaaaatcag aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact
    20281 gatggctgag atagacgaac gaacaatgca gctgcaagga gaaatcgacg gacaatcttg
    20341 gaatcatctc aactggtgaa attttcttat aaataacaaa gataaaaatg tgcacagcga
    20401 caattatttg ctacaataag aatatgaagc gtttgataac gctattatac ataaaaattg
    20461 gtggtgaaaa aaatgtatga tgaaaatgag tggggagaaa atgttcggat gacccgaaga
    20521 agacagcaaa aacaggcagc tcgtaagcgg atgatcatga ttggggcagc agttgccgtg
    20581 tttttgatca tagcaggagg agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa
    20641 gcgaaaagta cgaccataac ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt
    20701 gagtcatcaa ctaaaacaaa agagacacaa acttctgaag aaaaaacagc aaatggtttg
    20761 ccagagcaac ttcaagcaat cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct
    20821 gcattgattg aagaatacca gacagaaatt cagggaaatc aagagggaat agctggttta
    20881 tcagcagttg ctaaccaaaa agcacgtgag cttcaagcaa tctccgacga agggatcaga
    20941 aaattacgtg cagcgtatca ggcagcagaa aataaagacg gtgtagactt agatactttg
    21001 atcaatcaat tatcggctaa ttatacgaat catgtcgcta agatttccga tatttatttg
    21061 cagactagcg cctctcttca agcagagtca acaagcacac aagacacaac atcttctgat
    21121 tctgagacag cagagtcggt agaagcttct gaaatgcaag ctcgaacaac acaagacaca
    21181 acagattcct ctgaaactaa taccaacagc gcgacttcac aggctgcgac aaccgtagtt
    21241 ttggaaggag aaggtccaaa tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc
    21301 ttgtcgctga atgggatgag catggagaat tatttcctga ctcccggtga gacgctaaaa
    21361 ttgaattgat aaaagcaata aaaaagaacg aagcttccac taaggtgaag cttcgttctt
    21421 tttttatttt ccggttcttt tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa
    21481 aacgagtcca ataatcaatg agattctcgt ttcaggattg ataaacatga aaatcaatac
    21541 gatagctaaa aaagcaagtg tcacataatt cgtataagga gatagtggca ttttaaaagg
    21601 atgatcatgc attttatctg cgtgcttctt tttgaagcga atctcgctga tcaaaatcac
    21661 tacccaagga accataccag gcaaaatact tgagctgtat acgaaaacga aaatattttc
    21721 tgactggttg atgaacattg gtagaagata atttaagatc agaccaatta agattccacc
    21781 tgagattgct acgacaggat aaaaaggcgc accgtgacga gatagtttca gaaatttttt
    21841 agacatcttt ccatttatgg caagtgtata gaccatacga cttgcgctga aaatccctga
    21901 attgcaacct gataatgccg ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt
    21961 aatgccgact ttagaaaaag tttgtacaaa aggtgaacca atctcactca attgattcca
    22021 cggatagata ctcacaatga taaaaatagc accaatataa aaaatcaaga ttcgaccgat
    22081 agtggactgg acagcttcaa tgatcgtctt tttaggattc tctgcttcac cagcagtcat
    22141 tccgatcagt tcgattcctt gataggaagc aacaacgatc gataatgcaa agaaaaatcc
    22201 tttgacgcct ccagtgaaga atccaccatt tttccaaaga tttgaaatcc ctactgcctg
    22261 accatgatta ccaaatccaa ataagattac cccaagacct gcaatgatca tcaaaatgat
    22321 cgttatgact ttgatgatcg aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat
    22381 aagatttgcc agcataagaa acaagacgac aatgattccg ggaataattg ccggcaaatc
    22441 aggccaccaa taattcatat aagatcctac agcaatcact tcacttattc ctacgacaat
    22501 atattgaaaa atattactcc aagcggtcaa gtagccagcc acaggatgga tgtactcgct
    22561 cgcgtaattt gcaaaagaac cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg
    22621 cataatcaaa tacaaaaaga atcctgcaat cgcataggct cccatcactg agggccctgt
    22681 ccaacgtatg gttgaggagg cacccataaa cagaccgaca ccgatcgttc cacctagtgc
    22741 gatcatttgg atatgacggg cactaagact tcgagttaac ttttcttctt ccatacaaaa
    22801 actcctttcg ttgttgagtc aacaagaaaa aataatcaaa cgtttcaaca catgtttttc
    22861 attattaggt tagccttatt gaactcttga tgtgattcta cagaaaattt tcataaaatg
    22921 cgaggaaaat tcgaaaaaaa ctttttcatc attctacttg aaaatgcttt ccattttagt
    22981 taaactatat ttaaataaaa agttaggtaa acctaaaaag gagtgtaaga tttggaacgt
    23041 acggccattg taattcaaga cctttcagcc gcttatcaag ggaaaactgt tttgaaaact
    23101 atcaacttga cgataaatcc acaaaaaatt accggtatta tcggaccaaa cggtgcagga
    23161 aaatcaacat ttatgaaatc tttgctagag ttgataccag ctactactgg taaagtcacc
    23221 ttttctggac agccggtaaa ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt
    23281 gaattggatc tttcctttcc aattgatgta ttaggcgttg tacttttagg aacatatcca
    23341 tctttacgaa ttggacaaag acctgggaaa aaagaaaaag aacgtgcaag acaagctttg
    23401 aaaaaagtag ggatggaaga atatgccaaa agacagatca gcgaactatc gggtggacag
    23461 cttcagagag tttttattgc aagagctcta gcccaaggag cagaatggat ctttttagat
    23521 gaaccattcg tagggattga tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa
    23581 ttgaagaatt caggaaaaac gattttgatc gtccatcatg atcttcataa agtagaagaa
    23641 tatttcgatg aggttattct tttaaataaa cagctgatcg cttccggtcc agtacaagaa
    23701 gtctttacat cgaagaacct tcaattggcc tatggtgaga tcatcagaca tttagtaaaa
    23761 ggaggagaga aaaaatgatc caatccttta ttgatggatt aatgaattat caattcctgc
    23821 aatacgcttt ggtcacttcc atgcttgtag gacttgcttc tggagtcatc ggttctttta
    23881 ttattttacg agggatgtct ttgatgggag acgctatctc tcacgcagtt cttccaggtg
    23941 tagccatttc ttatatgttt ggttttagtt acatctttgg agccactgct tttgggatgc
    24001 taacagcagc agtgattggt ttcgtgactc agcggagccg attgaaaaat gatacagcga
    24061 ttggaattgt ctttagctcc ttttttgcat tagggattat cttgatttca tatgcccaga
    24121 gtgcgacaga tttgtatcat attttatttg ggaatgtctt agctgttagg gaaagcgatt
    24181 tgttgctgac agcactcgtt agtggaatcg tcttgatatt tgtctttttc ttttataaag
    24241 aattgaagat cacatcattc gatccgacaa tggcaaaagc ttatgggctg aacacatcgc
    24301 tgatccatta tcttttgatg ttctttttga cattagtggc tgtagtcagt ttacagacag
    24361 taggaacaat cttggtgatt gccatgttga tcacaccagc cgccacggct tacttgctaa
    24421 cgaaccattt actgaaaatg atcattacag ctgcaggaat cggtatgcta agtgcagttg
    24481 tcggtgtgtt tttcagttat agttacaatt ggccatcagg agctacgatc gtgttagcat
    24541 gtaccgcatt ttttatcctt gcttttttat tttctccaac aaaaggaatt ttatttcaga
    24601 aaggaagcag atagatgaaa acaaaaaaat cactcttttt aatcttggca gttagtttct
    24661 tggtattagc cggttgcgga aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa
    24721 agttgtcagt cgtggctacc aattcgatct tggcggacat ggcaaaagag gtcggaacag
    24781 atcaaataga tatccacagt atcgtaccag tcggaacaga tccgcatgaa tatgaagtac
    24841 taccagaaga catcaaaaag gcaagcgatg cagatgttat tttatacaac ggtttgaatc
    24901 ttgaaacagg taacagctgg ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa
    24961 aagattattt tgcagttagc aaaaatgtag aacctcttta tttaactagc ggtgaagaac
    25021 atacaaaagc agatccccat gcttggctag acctatctaa cggaataaaa tatgtggagg
    25081 aaatcgcacg tatattctct gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg
    25141 cagaagcata tgtggaaaaa ctaaaagaat tagatacaca agcgaaggaa agttttgctt
    25201 ctatcgaaga gaacaaaaaa ttattagtaa caagtgaagg ggcattcaag tatttttcac
    25261 gagcatatga tttgccagca gcttatatat gggagatcaa tacagaaagt caaggtacgc
    25321 ctgatcaaat gaaagcgatc attgatcaga taagagcaaa agaagtaccg gttttattcg
    25381 tggaaaccag tgtagactca agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa
    25441 tctatgataa actgttcact gattccatag caaaagaagg agaacaagga gattcttact
    25501 atcagatgat gaaatggaat attgaaacga tccatgaagg tttgtcacaa acaaaagaaa
    25561 gctgaaaagt gcaaggtcta gcttgaggag cagaccttgc attttttata agagatatga
    25621 aaaaaaatta tgctgttttt cgtcgtacag ataccttttt tgaagaaatt ccgttatagt
    25681 tagagtaagg aaggaaggtg tggctatgaa ttttacacgt aaagataaat atacgattaa
    25741 agaagaagtc tttcgattga tcagccagca ggaaaatacc gacaaggatc aagaccttca
    25801 atttcttgaa gcagaattag ctaaattatt catagcaaat caaaatgaga caacagaacc
    25861 agatgaagag ctgtacatag gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa
    25921 taccggtatc ggttattttg tgttcctttc tccagtcggc ttcacacctg gcgtattagt
    25981 taaacaaaac gacgaaataa ccgtcaaagc atggcctgca ttattttcag tcatcaaagc
    26041 agaaggagta atcaaaaaga tccagtcggc ctattataaa agtcaattta ttgccaacag
    26101 caaattaatg aatatgtttt ataatcgtct agtagaaatt gctaggcctg ggaaaatccc
    26161 gcaaacattc acgtacacac agcaaaaaaa tctctatgaa gaagtattgt attcccgcga
    26221 tctgtctgag ttttcgccat gggaagaggt aaggctccat gaactgttaa gtacaaaacc
    26281 agtttcattg aattttttta atgcattaga taacaaaaga aaattaattt caacaaatta
    26341 ttgggaagta tttgctgcaa gaaaaaaatg gatggaagaa aagaagattg ccgatcagca
    26401 gatccagttt gtccagctga aaaaagaaaa actatattat gggttataca gccgttaaca
    26461 gcaagcgagg aagaccatga cattaaaaga agaaatcata caagaaagca aacgattagg
    26521 aattgacaaa atcgggttca ccacggcgga accttttgat tcattaaaag aatcgttgga
    26581 agaacaaaaa gcagctggcc atacttctgg ctttgaacat ccaaatatcg atgagcgtct
    26641 ttatccagaa atgacatttg atcagccgaa atcgatcatt tccattgcac ttgcgtatcc
    26701 tactaggatc catgaagaag tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc
    26761 ttgggggatc gattaccatg atatactcag agaccgtttg aaccgattga tcgaattcat
    26821 ccaaacaaga gcagaaaaat ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga
    26881 tactggagaa ctgatcgatg tcgctgtagc acagcgagct ggtttaggat ttattggacg
    26941 aaacggattg ttgatcactg aagagttcgg ttcatttgtc tatcttggtg agatcgttac
    27001 taatattgta tttgagcctg ataaaccagg agaatttggt tgcggtgact gtatgcgctg
    27061 cgtgactgct tgtccgacaa aagcattgct tggtgacgga agaatgaatg cccagcgatg
    27121 tttgtcttat caaactcaga caaaagggat gatgccggaa gaatatcgta aaaaaatgcg
    27181 aaatgtcatt tatggatgtg atatctgcca gttagtctgc ccttacaatc aaggaaaaga
    27241 ttttcatttt catgaagaga tggaaccaaa agtagacgaa gtatatccaa aattaaagcc
    27301 aatgctttcg atgtctaata aagacttcaa acagcaattt gggcatttag ctggttcatg
    27361 gcgtgggaag aaacctttgc agagaaacgc attgattgct ttagctaatc taggtgatcg
    27421 aacggcattg cctgagattt cagaatgttt aacagatgtc cgccctgtca tcagaggaac
    27481 agcagcttgg gctattggac gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt
    27541 agatgaaata ttagctaaag aaacagaaga agtcgttata aacgaattgg aacatgccca
    27601 gaaattattg cggagaaagt taaaataaaa aaatagttaa cattattcaa agttttt
//
LOCUS       DAZULO010000027        26408 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000027 DAZULO010000000
VERSION     DAZULO010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.028"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(280..648)
                     /locus_tag="ACG8OA_001169"
     CDS             complement(280..648)
                     /locus_tag="ACG8OA_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFS4975994.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            798..1280
                     /locus_tag="ACG8OA_001170"
     CDS             798..1280
                     /locus_tag="ACG8OA_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFS4975995.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1317..2327
                     /locus_tag="ACG8OA_001171"
     CDS             1317..2327
                     /locus_tag="ACG8OA_001171"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFS4975996.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2442..3155)
                     /locus_tag="ACG8OA_001172"
     CDS             complement(2442..3155)
                     /locus_tag="ACG8OA_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4975997.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(3328..3935)
                     /locus_tag="ACG8OA_001173"
                     /pseudo
     CDS             complement(3328..3935)
                     /locus_tag="ACG8OA_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            4419..4646
                     /locus_tag="ACG8OA_001174"
     CDS             4419..4646
                     /locus_tag="ACG8OA_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFS4975998.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4662..5828
                     /gene="hemW"
                     /locus_tag="ACG8OA_001175"
     CDS             4662..5828
                     /gene="hemW"
                     /locus_tag="ACG8OA_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFS4975999.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6062..7105
                     /gene="hrcA"
                     /locus_tag="ACG8OA_001176"
     CDS             6062..7105
                     /gene="hrcA"
                     /locus_tag="ACG8OA_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFS4976000.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7199..7762
                     /gene="grpE"
                     /locus_tag="ACG8OA_001177"
     CDS             7199..7762
                     /gene="grpE"
                     /locus_tag="ACG8OA_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFS4976001.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7813..9642
                     /gene="dnaK"
                     /locus_tag="ACG8OA_001178"
     CDS             7813..9642
                     /gene="dnaK"
                     /locus_tag="ACG8OA_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFS4976002.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9793..10959
                     /gene="dnaJ"
                     /locus_tag="ACG8OA_001179"
     CDS             9793..10959
                     /gene="dnaJ"
                     /locus_tag="ACG8OA_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFS4976003.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(11041..11670)
                     /locus_tag="ACG8OA_001180"
     CDS             complement(11041..11670)
                     /locus_tag="ACG8OA_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4976004.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            11682..11855
                     /locus_tag="ACG8OA_001181"
     CDS             11682..11855
                     /locus_tag="ACG8OA_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976005.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11900..12637)
                     /locus_tag="ACG8OA_001182"
     CDS             complement(11900..12637)
                     /locus_tag="ACG8OA_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4976006.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12639..14141)
                     /locus_tag="ACG8OA_001183"
     CDS             complement(12639..14141)
                     /locus_tag="ACG8OA_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFS4976007.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            14400..16394
                     /gene="uvrB"
                     /locus_tag="ACG8OA_001184"
     CDS             14400..16394
                     /gene="uvrB"
                     /locus_tag="ACG8OA_001184"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFS4976008.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            16404..19220
                     /gene="uvrA"
                     /locus_tag="ACG8OA_001185"
     CDS             16404..19220
                     /gene="uvrA"
                     /locus_tag="ACG8OA_001185"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFS4976009.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            19483..20748
                     /locus_tag="ACG8OA_001186"
     CDS             19483..20748
                     /locus_tag="ACG8OA_001186"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFS4976010.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            20752..21483
                     /locus_tag="ACG8OA_001187"
     CDS             20752..21483
                     /locus_tag="ACG8OA_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFS4976011.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            21618..22502
                     /gene="rapZ"
                     /locus_tag="ACG8OA_001188"
     CDS             21618..22502
                     /gene="rapZ"
                     /locus_tag="ACG8OA_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFS4976012.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            22499..23497
                     /gene="yvcK"
                     /locus_tag="ACG8OA_001189"
     CDS             22499..23497
                     /gene="yvcK"
                     /locus_tag="ACG8OA_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFS4976013.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            23531..24466
                     /gene="whiA"
                     /locus_tag="ACG8OA_001190"
     CDS             23531..24466
                     /gene="whiA"
                     /locus_tag="ACG8OA_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFS4976014.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            25009..25659
                     /locus_tag="ACG8OA_001191"
     CDS             25009..25659
                     /locus_tag="ACG8OA_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976015.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            25846..>26408
                     /locus_tag="ACG8OA_001192"
     CDS             25846..>26408
                     /locus_tag="ACG8OA_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFS4976016.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT         8720 a         4454 c         5609 g         7625 t
ORIGIN      
        1 tgggcagtat tatgaaagat cactgatatt ttctcgtatt ttttccttat tcaaaagttt
       61 tgcaggaaaa ttgctgaaaa aataatttta ctatgtagaa aattcaaagc cattcaatca
      121 gtgatttttt tcagcacaag aaagactcct tataaaaata gcagatactt tgtcaattaa
      181 gtgtttaacc agggatgata aaaaggatag gattgaacaa aacctgtata aggtgatgaa
      241 tagtttcctc gccagtaaaa ggtttattcg tataaagctt tatcgccaaa atttatcatg
      301 acctttttta tcttcaatat taaatgaaat caattgttca atcaattccc agttgattac
      361 acttttccaa ggaactttga tgacgttatc cgtatatacg tagcctgctt cttggatatt
      421 tttttcaagg gcacgaatcg ctgctttttc cggtgcgata gaaaaatgat ttttagcaaa
      481 actaaatgca ataataaagg taccatggtc agtaaacata ggttggttcc attttacagt
      541 tgtgtctagc tggggatacg tttctgttat cttagcaaaa aggtctttga tcttttgttg
      601 atgttctttt tctttgattt gattgatgat cggctgaaat ttttccattc taaaacgcct
      661 ccattaagtg gtttgattta ttttaccaga aaaacaatag ttatgtataa aaaacgaaca
      721 aaacacaagt aatgttgtaa ccgctatatt tatatgataa gatgtctcaa ataataaaat
      781 agaaaagaga cataaaaatg attgacttgg caaagaaatt cttacacgaa aatatatatt
      841 ttgagttgta tcatcatcga gccatttata caaatgaaga tgctttggta ataaagaaag
      901 aacaaggatt tacaggtaca gagaccaaaa gcttattctt aaaagataaa aaagataata
      961 actacgtata tctgactttt actacgaaac aaacggattt caaagcatta gggaagatcg
     1021 tcggtaaaag gctttctatt gtttcatcag aacgcatgga agaacaaacg ggacaaaagc
     1081 agggcgctgt ttctccgttt ggttatgaaa aacagattcc gattattatt gatgaagagc
     1141 tcttatcaaa tgaaaaactt gtttttgcac caggtagacc agatcaaacg atggtcatcc
     1201 aaacggttga cttaaacaaa atcatccagc tacttgagat gccgatattt ttgatgccgg
     1261 caaacgaaaa aaacaattaa ccaagtaaaa tagtgcatac ttggaggtga agcaacatga
     1321 taagttatcc attattagaa agaaatgcaa cgattggggt tactgcacca tcctcaggcg
     1381 tatcagagga acaccattcg ttactcgaat cagctttaaa gagaatggaa gaaaaatctt
     1441 tccaagtagt tactggaaag acgctttgga cgcaagataa ggaaaaatca ggtccagcaa
     1501 gaaaacgagc agatgaattc aatcaaatga tgcaagatga aaatatccaa atgatctttc
     1561 cgacttgggg aggagaatta ttgattgaga ttctcgattt tgtcgatttt gatgctctac
     1621 aaccaaaatg gatagtaggt tattcagata ttagtacgtt gctacttgct gtcacattaa
     1681 aaacagggat agccactgca catggaacta accttattga tttaagagga gagtacttgg
     1741 atgaaacgac agcaatgtgg gaaagtgtac tgaaaacaag agaaggagaa tcgattctac
     1801 agcaatcttc tgaaaagtat caaaaagtgt ggccccatga gcattcatca tcttctgttt
     1861 tccatctcac tgaaccaaca aattggcaat cagtcagcgg agatgcagta aggatgaagg
     1921 gacgattatt aggtggttgt attgatacga tcaggcattt aatcggtact ccttatggag
     1981 atgtccaatc ttttaaaaga aagtataatg atggaaagcc gatcttgtgg tttctagaaa
     2041 actgtgagct atcgtcagtt gatttaagaa gatcacttgt tcagatgaaa ctggcaggct
     2101 ggtttgatgg cagctcgggg atcatgttcg gtagaagtgc ggcagaggat ttgttagatt
     2161 ataaaatgaa ggatgtatac gaagatctgt caaaggaatt aaaactaccg atcttgtatg
     2221 atattgattg tggtcatgta ccgccacaac tgacattgat taatggtgcc tatgcagaag
     2281 tagcggtaga agatagaaaa gcgactgttt tgcaaacgtt tatttaaaaa attttttcct
     2341 gccttggagt ctatcaaaga ccaataaatc taaatagaaa agaaagtgaa agaagctgac
     2401 aaaatgctgt cagcttcttt ttgtataaca tattagaaag tctaattatc tataactgtc
     2461 acatcgatta actgtttcac gtcattggga tttcctgtag gaatagctac tacagatgca
     2521 ttatcaatga tttcttgagt tgtttctcta atgaacgctg gataagatgt ttgatctgca
     2581 ggcatttgcc aatgtgaagg aactggataa ctagtgattg tcccgtcatg gttacttgaa
     2641 tacactaaaa ctccctggta actgtatttc ccagtgagca taaccacatc tttcggatag
     2701 actgcactcc ctgcatcata aggggcgata ggtgttcctg caggataatg aataacatgt
     2761 aattcgaaat cacccgatgt atctctaaaa gtattacctc taatagctag ccaaacacgt
     2821 gcgtactcaa tttgtagatc agaatatcct gctaacggat ctattgactg agaagactgt
     2881 gaggaggttg tgtctattgt tgaagaagaa atatttgtct caactgtact actagatgta
     2941 gaagaagtat tgggctctga gactttactg cttgttgaag atgtattcgt ttcagtagaa
     3001 gctgatttct gagtacttga tttttcagct gaactggttt gtctgattga acttttgtct
     3061 gtggtcgcag atggatcact attttcttta ctaggagacg tacatcctac aaggaataaa
     3121 gagaccaaca ataaaataaa acctaacttt ttcatataaa atacctcatt atttttttaa
     3181 taccagtata gcataatcct gaatttttga atccgtaaaa ccttgtctat gaatataaat
     3241 agagtgtatc ttctttgata aatgtcagaa aaagtgtgac aaataatcat cagtaaaatt
     3301 acttgtcaca ctattgataa ggagtattta attgaagaga atgattagga gaaaggatct
     3361 ccgtactttt tatagtaatc atagaatcca agtttgtata gacgaatcgt tttttctctt
     3421 atttcttctg tttcaaaatg gactgcttga agaatcaagt ctgtgaattc tataggagct
     3481 gttccattcg cagtaatcaa attacgatct ttcacagctt gttgttctat aaaatcttct
     3541 ttattctgat aattttccga ttctaaccaa ggaaattgcg catttccggt gtgtttgtaa
     3601 ccttgcaata atccgctctg agctaaataa tcaactgccc cacaaatagc agctactggt
     3661 acgtctgttt ttaacaattt atcgattatt tgatataaat gatgattttt tatgtcccat
     3721 gaattgccac cgatcataat aaaaaggtca actttttcag gtatttgttc gattaagtaa
     3781 tcgatttttg tttttaatcc tccaatggaa gtgacctctg attggataga agcagttttg
     3841 ttttgccatt tctcattttg gtttagtgtg ctagtaatat aagcaccttc ccaatcggca
     3901 tattcctcta atagaaacgt aatcgcagtt ttcattttca agctctcctt tactatctat
     3961 aacaaggaca acagttttaa cctacagaca ttcattaaaa ttgggtagcg acagcgaaaa
     4021 caacaattct taagttttag acagagtata cagatagtca acttaattaa atacgaacta
     4081 aataaaaatg acaggtaatc atttttgttt accagcatta taacattttg catacatgga
     4141 tgacaaaaga accaattccc acttgcttga gtacaatatt aaaagaaact ggaaggcaaa
     4201 aagtaattat agaaacgaaa aataaatcat atcgggcact cagacatgaa aacaacccta
     4261 gagatatata actaggtaac cagtttaatg aaagaaaaaa tcatccaaga agtcgattct
     4321 tggatttttt agtggatttg ttcgccaaat acgtttagta tgcgtatatg agaggttgta
     4381 agcaaagcgt tgtggtctga gcacttaagg aggaatctgt gaaaatagct tttcatattt
     4441 ttgcagattt tgacttaatg ccgaaggtca gctttgcgat taccgtttat cgagaggctg
     4501 taaaagcacc gtgtagctcc gatgacaata agcaagcatt tttagagcta ggtttccttg
     4561 attctaaata tgcggcttat ttcaatgagt tggtgcttga acgcgatttt aagaaggtcg
     4621 aaaacaggca cgacttttcg acgtaaggag aaagaatttc aatgatagaa acaataaatc
     4681 ccgcagaaaa caaggtttct gcgtacattc atatcccatt ttgcgagcat atctgctatt
     4741 actgtgattt taataaagtt tttttagaag ggcagccagt agatgagtat gtggaaatgc
     4801 tgctgaatga aatgaaaata atgctagaaa gtcatccgac agcgaattta gaaacacttt
     4861 acgtaggggg cgggacacca acttctttat ctgctaaaca acttgatcga ctgttatcag
     4921 gtgctagaga gatcctgcct tttcaaaatg gtaatgagtt tactgtagaa gcgaatccag
     4981 gtgatttgac ccgtgaaaaa cttcaagtaa tgaaaaacta tggcgtcaat cgtttatcca
     5041 tgggagtgca gacatttgat aatcgcttat tgaaaaaaat tggacgtaaa cacaccgcag
     5101 aagatgtgta tcaaactatg caatttttag aagcagaaaa ttttaccaat gtaagcatcg
     5161 atttgattta tgcattaccg ggacaaacac tagaaggata tctggatact ttggaaaaag
     5221 ctttggcttt ggatctgcct cactattctt tgtactcatt gatcctcgaa aacaaaacaa
     5281 tgttcatgaa ctgggtacga caaggaagac tcgaactgcc tgatcaggaa acggaaacca
     5341 gaatgtttga agagacgatt gaagcaatgg aaaagcatgg acgtcaccaa tatgaaataa
     5401 gtaactttgg gctctccgga catgaatctc aacataacct catgtactgg aacaatgacc
     5461 actattttgg gtttggagct ggtgctagcg gttatcttgg agatattcgt taccgcaaca
     5521 aaggaccaat ccaacactat ttacgcccat tacgagcaaa tgaacgcccg gtattggaag
     5581 aagaaaaatt gacacgaaaa aatcaaatag aagaagaaat gttcttagga ctaagaaaaa
     5641 aaacagggat tactaaaaaa catttctatg atcgctatca aaaaacaatt gaatcggtgt
     5701 acggtgatgt gctaagggaa ttagagcaag aagggttact tgttaatgaa gaagaccgtg
     5761 tttatttgac accaagaggg acctttttgg ggaacaatgt gtttgagcgc tttttgtttg
     5821 aaaaatagtt taaagaatat ttgtttttta tctgaattta aataaaaaaa gttcaaaaat
     5881 acaataaagg actattgaaa cgttgatata actggattta catcaattat ttagaattag
     5941 cactcttgtg tttagagtgc taaaaaatgg agtctttctc ttgacaggag aagggcttct
     6001 tgctatattt ataagtggat tagcacttag aatatatgag tgctaatgag aggtgaggaa
     6061 gatgttaaca aagcgacaaa acgacatttt acgtctgatc atccaacact atacgagtag
     6121 tggcgtgccc gttggttcca aaacattgat gtctgaggga gtcaaggcca gctcagcaac
     6181 gattcgcaat gatatgaaag cattagaaga tgaaggattg ctgttgaaga ctcactcttc
     6241 ttctgggcgg attccatctg ctttaggcta tcgttattac gtggatcatt tgttaaaacc
     6301 tgctcgcgta gcagaagatg atttgctgct gattcgtcag tctctaggca gagaatttca
     6361 tgaaatcaat gagattatta agcaatctgc tgagatatta tctgagctga caagctatac
     6421 gactttttca ttgggacctg aaataaaaga ccgccgactg actggctttc gtatcgtacc
     6481 cttgaataat cgacagatta ttgctatcgt cgtaacagat atggggaatg tagaaagcca
     6541 agtgttcact cttcccgaat atatgggaag tcaggaacta gaaaaaatgg tacagattgt
     6601 aaacgaccgt ctagtcggag atcctctgat gacggtgtac cataaacttc gtacggagat
     6661 tccgatgatt cttcataaat atttccaaac gacagaaggg atgtctcagt tatttgacac
     6721 cgttcttggt catgtttttg aagataaggt ttatgtcagt ggacaaatga atctcttgga
     6781 ttatgagccc cgtcaagatc ttgatcagtt caaatcgatg ttttctttta tgaaagactc
     6841 agacggactt acacaaatga tcactccgat ggatagcaat attcatatca agattggtgc
     6901 tgaattaggc aatgatttat tgcagaatat gagtatgatt caagcaagtt acgagataga
     6961 tggtcatgga agaggtacaa tagccttgtt agggcctact agtatgcctt attctcggat
     7021 gttcggtcta gtcgatgttt atcgaagaga actagcagct aaacttgctg attactaccg
     7081 ttcgcttgac ttgtcaaact cgtaatgtga ataacgaagc tatttgcagc tgatcgttct
     7141 ctctttctta gtaagagcaa tcaaacagct gactgaataa tgaaaggaag actgtatcgt
     7201 gaaagaaaat caagaagaac tagataaaga aatgacagat gctcagccag aaccggaaat
     7261 cgatgtcgaa gctgcagaag attcgggtat ttcagaagct gaagcggaag aattcgaaac
     7321 agcgaaatta aaagctgagt tagaagaaat ggaagataaa tacttgcggg ctcgtgctga
     7381 aatcgcaaat atggcaaatc gaggaaaaaa tgagcgtgag caattacaaa aataccgttc
     7441 acaagattta gccaaaaaat tacttccatc aattgacaac ctggaacgtg cattagccac
     7501 agaagtgtca gatgatcaag gggcaggttt gaaaaaaggc gtggaaatgg tcttggaaag
     7561 tttgcgtaac gcactggaag aagaaggaat tgaaaaaatc cctgctaaag gagaagcctt
     7621 tgatcctaat cttcatcaag cagtacaaac tgttccagcg actgaagata caccagccga
     7681 tacgattgta gaagtactac aagaaggcta caaattacat gatcgcgtgt taagaccaac
     7741 catggtcatt gttgcacaat aattgtgcgg atataaaact tacactttca attaaagaag
     7801 gagacgttaa ttatgagtaa aattatcggt attgacttag gtacgactaa ctctgcagta
     7861 gcagtattgg aaggaaacga agcaaaaatc atcgctaacc cagaaggaaa ccgtacaaca
     7921 ccttctgttg tatcattcaa aaatggagaa atccaagtag gggaagttgc aaaacgtcaa
     7981 gctgtgacaa acccaaatac gatttcatca atcaaacgcc atatcggtga agcaggttac
     8041 aaagtagatg tagaaggtaa atcatataca ccacaagaaa tctcagcaat gatccttcaa
     8101 tatatcaaag gattcgcaga agactattta ggtgaaaaag tcgaaaaagc agttatcact
     8161 gtgccagctt actttaatga tgcacaacgt caagcaacaa aagatgctgg taagatcgct
     8221 ggtctagaag tagaacgtat cgtcaatgaa ccaactgcag ctgctcttgc ctatggttta
     8281 gataaaacag atcgcgacga aaaaatctta gtattcgacc ttggtggcgg tacttttgac
     8341 gtatctatcc ttgaactagg agacggtgtc tttgatgtat tgtcaacagc tggcgacaac
     8401 catctaggtg gggacgactt tgataacaaa atcatcgact acatggtagc agaattcaaa
     8461 aaagaaaaca gcattgactt gtctcaagac aaaatggctt tacaacgctt gaaagacgct
     8521 gctgaaaaag cgaaaaaaga tttatctggc gtatcaagca cacaaatcag cttgccattt
     8581 atcacagcag gagaatctgg accattgcat ttggaaatga cattgacacg tgcaaaattt
     8641 gatgaattaa cagctgactt agtagaacgt acaaaagtac cagtacgtca agcgctaaaa
     8701 gatgcaggtt tatcacaatc agaaatcgac gaagttattt tagtcggtgg atctactcgt
     8761 atccctgcag ttgtagaagc agtacgtaaa gaaacaggaa aagaaccaaa caaatcagtt
     8821 aaccctgatg aagtagttgc tatgggtgct gctatccaag gtggcgtgat cactggtgat
     8881 gtgaaagatg ttgttttact tgacgttaca ccattgtcat taggtatcga aacaatgggt
     8941 ggagtgttca ctaaattgat tgaccgcaat acaacgattc caacaagtaa atcacaagta
     9001 ttctcaactg cggcagataa tcaaccagca gtagacatcc atgtattaca aggtgaacgc
     9061 ccaatggctg ctgacaacaa aactctagga agattccaat tgacagatat tccagcagct
     9121 cctcgtggta ttccacaaat cgaagtaaca ttcgatattg ataaaaacgg gattgtaaat
     9181 gtatctgcga aagatctagg tacacaaaaa gaacaaaaaa tcacgatcaa atcttcttca
     9241 ggtctaacag acgaagaaat cgaacgtatg gtaaaagatg cagaagcaaa tgcagaagca
     9301 gataaagctc gtaaagagga agttgaccta cgtaatgatg tagatgcatt gctattctct
     9361 gtggacaaaa cattgaaaga attagaaggc aaagtggacg aagaagaagt gaaaaaagca
     9421 gaaacagctc gcgatgaatt gaaagccgct gttgaagcaa acaacatcga agaaatgaaa
     9481 actaaacgcg atgcattgaa cgaaatcgtt caaaacttga ctgttaaact atacgaacaa
     9541 gcggcacaac aacaagctca agaaaaccca gaagcagctc aaggcggagc agatgatgtc
     9601 gttgatgctg attttgaaga agtcgatggc gacgataaat aatcagttca ctgaatttcg
     9661 agagaaggcc aaagttgcta ctttggcttt ttcttttgaa aaatagtttg aagtatcggt
     9721 ttttgtttca aacaaaatat gatatgataa caagcgacgt gaaaatgatg agaaccaata
     9781 ggaggaaagc ctatggcaac aaaacgtgat tattatgaag tcttagggct ctcaaaagga
     9841 gcatcagaag atgaaataaa gaaagcttat cgtaagcttt cgaaaaaata ccatcctgac
     9901 atcaataaag aacctgatgc ggaagaaaaa ttcaaagaag tatcagaagc atatgagatt
     9961 ttaagtgacc cgcaaaaacg cgctgcatat gaccaatacg ggcatgcagg cacagatcca
    10021 aattacggtg ctggtggagc tggtggcgga tttggtggat tcggcggctt ctctggtggc
    10081 ggcttcggtg gttttgaaga tatcttcgac tctttctttg gaggaggcgg tcggtcagtc
    10141 gatccaaatg cgcctcgtca aggtgctgat ttgcaatata cggtcgactt gtcttttgaa
    10201 gaagcgattt ttggtgtaga aaaagaaatc aagtataatc gggaagaaat ctgtcatact
    10261 tgtggtggga acggtgcaaa accaggcaca caaccaacta cttgtcataa atgtcatggt
    10321 gcaggtacga ttaatgtaga acgccaaaca ccacttggac gagtgatgag ccgtcaaact
    10381 tgtgatgttt gtcatgggac agggaaagaa atcaaagaac cttgtccaac ctgtcatggc
    10441 tcaggacatg agaagaaagc acattcagtt aaagtgaatg taccagctgg tgtagaagaa
    10501 ggacaacaaa tgcgtttagc tggtcaagga gaagctggag aaaacggcgg accatttggt
    10561 gatttgtatg tggtcttccg tgtagaagaa agtgatatct ttgatcgtga aggttctgaa
    10621 atttactatg aattgccttt aagttttgta caagcagctt tgggggatga agtccaagta
    10681 ccaactgtcc atggagatgt gaaattgaag atccctgctg gaacacagac aggtacgaac
    10741 ttccgtttac gtggaaaagg cgctccgaag ttacgtggtg gtgcaacagg cgaccaacat
    10801 gtcaaagtaa aactgatcac accgaaaaat ctaaatgaac aacaaagaga agcattacgt
    10861 gcattcgctg aagctggcgg catcaaagtt gaagaacagc aagaagacgg tttcttcgat
    10921 aagtttaaag atgcttttgg cgggaaaaag cggaagtagt ttttctagta aaaacgctaa
    10981 gtcattaata attaaaaacc agacgaacaa tcatatgttc gcctggtttt tttgtagttg
    11041 tcatgtttgt accaacaaaa aagccagctt tagaatatga taatcgtcat agtttctagg
    11101 ggtataacca gtgagagaat ggaacttgtt caatcgatat tgcaaggtgt ttttatgaat
    11161 aaataattct tctgagcaat ttttgatgct tccgttattt ttactgaagc atgccaaagt
    11221 cttcttgaac aaatgtactt cttcttctgt caggcttttc aaaatacgtt ttacatataa
    11281 tgttcgttga tctgacggga ttgctggtaa aacgatcccg aggtctaaat cttcaaaaaa
    11341 gcggatctcg ttcacagatt gatggaaagc tatccattcg caaacttgtt tggcatgaag
    11401 gaaagattgc ggtgtctctt gctctgtata acaaagcgac ccaattccaa agtttagctg
    11461 taactgatac attttattaa actttgtctg gattttatca agcaatgata gcaatgaggc
    11521 tctggaaatc cgattgacta ggataatgat ttgttgtcta tgtacagtga gcaaagcctg
    11581 tccgtaataa gagaagaccc tttcgagcaa ctccaataaa ggattgatcc gttcttgaca
    11641 caattgctga ttgtcagaaa gttgtcccac aactacataa tatggagaag aaaaatcaag
    11701 ctcaattaag tgagggtggt atcgactatg tacttgatat catcaatgaa ccgatggtac
    11761 tttcttatgc gatggaacag gtagagcgat tgcttgtttg tgcaggaaaa caagcgatgc
    11821 ggctgatacg attaggtgag aaaataagaa gataaaaata cacggtccct tcatggcgac
    11881 cgtgcatttt tggtttttat taaatattta atactttatt caagaaatct tttgtccgtt
    11941 cattttgcgg attttcaaag atttgttgag gcgttccatc ttctaaaaag tttccgtcgt
    12001 caatgaacat gacgcgattg gccacttctt tagcgaaccc catttcgtgc gtaacgataa
    12061 ccatcgtcat accttgttct gctaactttt tcatgacgtt caagacatca ccgaccattt
    12121 caggatcgag tgcagaagta ggctcatcaa aaagcatgat gtcagggttc atcgctagag
    12181 cacgagcaat agccacacgc tgtttttgtc caccagaaag catttcagga tacatatctt
    12241 ttttatcttc tagaccaact gtctgcagta atttgattgc ttttttctct gcttcttctc
    12301 tagattcttt cttcaggtcc atcggtgcta aaatgatgtt ttccaaaata gaaagatgag
    12361 ggaataggtt aaagtgttgg aataccattc cgatatgttg tctcaccaag ttgatatctg
    12421 tgcttttatc catcaagtgg gcgccgtcaa taataatttc accgtcagaa ggttcttcta
    12481 aacgattcaa gcaacgcaag aatgtacttt ttcctgaacc tgaagggcca atgatacaga
    12541 cgacatctcc ttcgttgatg gatacattga tgtcgtttaa tactgtgtta tcgccgtatt
    12601 ttttgactaa atgctcaact aagatttttt ctgccatctt acttcacctt cttttctaaa
    12661 acttttgcta attttgtcaa tgctgtgatc aaaatcaagt acatgatcgc aatgatcaag
    12721 taaacatacg tactttgtgt tgtacgtgca acaatgatct taccagtttg cagcaattcg
    12781 actacaccga ttgcggaaat gatcgttgtg tcttttaatg agataacgaa ctgattgacg
    12841 aatgatggga tcatgatgcg tatcgcttgc ggcaaaatga ttttttgcat tgtccgatta
    12901 taagaaagac ctaaacttct ggaagcttcc atttgtccga caggaacggc gttgatcccg
    12961 cctcgaacaa tctctgcgat ataagcactt gcgttaagag tcaaggtgat aacaccagca
    13021 aggaagtcag gaatcgtaaa tccaacgata ccaggcaacc caaagaaaat aaagaacgca
    13081 agaaccatca ttggaattcc tcggatgata tcgacataaa tactagcaat tgtgcgaaga
    13141 gcgcgtattg gtgctacact gaataatcca aaaatcacac cgattactat agccaaagca
    13201 aaggagatca aagttaggag aattgttctc caaagtcctt tcaacaatgc ttgatagttg
    13261 ttttggataa gtccaagaaa tgttgattca tcagcagtgg aagactcact ggaagtttct
    13321 gaaccatccg ctacatattc tttaataatc ttatcgtatt cgcctgttct ttttaattct
    13381 ttcaatcctt cattgaacat attaagaagc tcaggatttt gtcctttttt aacggcaaat
    13441 gcatattttc cgccagattc ttttttgatc ggtgtttcca attcttgtcc ttgtttgatc
    13501 gcataaccga ttaccggata atcatccatc attgcatcga ttgcgccaac ttttaacccg
    13561 tcgtacaatt gatcagcagc atcaaaatat ttgatagaat agccatattc agattcatgg
    13621 tcgcttaaaa attcagcact ttcggtacca acttttgctc cgactgtttt tcctttcaaa
    13681 tcttcataag acttgatcga agtatctcct tttttgacag ctaattggat cccgctatca
    13741 aagtaagaat cagaaaaatc atagcttttc tttcgttcat ctgttacagt cattccggca
    13801 atcattccgt ctgcttgtcc tgattccaaa gcttgcaaag cagcagagaa accacggtag
    13861 tccattcgga tgttaaatcc ttgcagtttg gcgatgtttt ccatcaattt tacatcgatg
    13921 ccttcgtact cgccctttgc gttttgaaat tcaaatggag caaaagcaga gtcactggca
    13981 attacgtata cgtctttttt gggctctatt ttttgcattg tctcgcttgt gcttgactct
    14041 gccgttgtag tgttgtcagc ttgtgcaaca tgaccaaacg caaatgtcac aagtcctgtc
    14101 attaagaaaa caaatgagtg aaataaatgc tttcttttca taatcaacct cctaaatttc
    14161 caatatttta ttattatagg cgaaaagtgc tgaatcagac aagactgtaa gctcgaaaaa
    14221 atgaaaaaaa tgcgagtttc taacatttta tgttataaaa cataacatgt ataataagat
    14281 aaaaaagtgg cgaaagtatg ttcggtgtgg tataattttt tagtaaggaa tgcctttgct
    14341 ttccttttct ttttattggt cgtaaaatga aaaggaatga atgaaaagga ggacgaagaa
    14401 tgattgagag agatacctcc cgacactttg agcttgtctc gaaataccaa ccggctggtg
    14461 atcagcctga agcgattaac caattagtgg atggcgtagt cggcgggaaa aaagcacaaa
    14521 tcttattagg tgcgactgga acagggaaaa catatacgat ttccaatctt attgagaaag
    14581 tgaataaacc aacactgatt attgcccata acaaaacatt ggctggtcag ctgtacgggg
    14641 aattcaagga atttttccct aacaatgctg ttgaatattt tgtttcttat tatgattatt
    14701 atcagcctga agcttatgtg ccttcaagcg atacgtatat cgaaaaagat tcaagtgtaa
    14761 atgatgagat cgacaaatta cgacattcgg ctactagttc attattagaa agaaatgatg
    14821 tgatcgtcat tgcatccgtt tcatgtattt ttggattagg ctcgcctttt gagtaccaaa
    14881 aacaagtagt ttcgattcga cagggagcag aactagatcg caatcagttg attcgtgact
    14941 tagtcagtat ccaattcgaa agaaatgata tagattttca gcgcggtcgt tttcgagtgc
    15001 gcggtgatgt cgtggagatt tttccggctt caagagatga acgtgcctta cgtgtggagt
    15061 tttttggaga tgaggtcgaa cgtatccgag aagtcaatgc actgacaggt gaagttttag
    15121 gagaaacgga acacgtagca atcttcccgg cgactcactt tgtgacgaat gatgaacata
    15181 tggagcatgc cgtagcgaat atcaaagcgg aattagaaca gcgtttaact gtcttgcgaa
    15241 atgaaaacaa acttctggaa gctcaacgtt tagaacagcg aaccaattac gatattgaaa
    15301 tgatgttaga aatgggctac acgtcgggaa ttgaaaatta ttctcggcat atggacggac
    15361 gtaaagaagg tgaaccgcct tatacgctat tggatttctt tcctgaagat tttttgatcg
    15421 tggcggacga atcgcacgta accatgcctc aaatcagagg aatgtataat ggtgaccgag
    15481 cgagaaaaca gatgctagta gattacggtt tccgtttgcc atctgcttta gacaatcggc
    15541 cacttcggtt agaagagttt gaaaaacatg tcaatcagat catttatgtc tctgcgacgc
    15601 caggaccata tgaacatgaa caaacagata ccgttatcca acaaatcatc cgtccgacag
    15661 ggctgcttga cccagtgatc gaagttcgtc caatcatggg acaaatcgat gatctagtag
    15721 gcgaaataaa tgaacgagtc gaaaaagacc agcgggtgtt tgtaacgaca ttgacgaaaa
    15781 aaatggcaga agacctcaca gattatttca aagaattagg gatcaaagtc aaatatttgc
    15841 atagtgatat caaaacgctg gaacgaacag aaatcattcg agatcttcga ttaggtgaat
    15901 ttgatgtgtt agttggaatc aatttgcttc gtgaaggatt agacgtacct gaagtttccc
    15961 ttgttgctat cttggatgcg gataaagaag gctttttacg tagtgaacgt tccttagtcc
    16021 aaacgattgg acgagctgct cggaatgaag aaggtaaagt aatcatgtac gcagacaaag
    16081 taacagattc tatgcgtcta gcgatggatg aaacgtcaag acgtcgtacg attcagcaga
    16141 agtacaatga agaacatggg atcgttccaa aaacgatcat taaagaaatc cgagatttga
    16201 tttctattac gaaagaatcg gaagatgata caaaagaagc ggtacaagtc tcgtacgaag
    16261 aaatgacgaa ggaagaaaaa gacacgttat tgatgaaatt agaaaaagaa atgaaagatg
    16321 cggcaaaagc tttggatttt gaaacagccg cgaatgtgcg agatatgatt ttagaattaa
    16381 aagcttcaaa gtaggaggac aacatggcta acgataaaat tacgattcat ggtgcacgtg
    16441 cccataactt gaaaaatata gatgtaacga ttccacgaga taaattcgtg gtcgttacag
    16501 gtttgtccgg ttctggaaaa agttccctag catttgatac gctgtacgct gaagggcagc
    16561 ggagatacgt agagagcctt tctgcttatg ctcgtcagtt tttaggacaa atggataagc
    16621 cggacgtaga tagcatcgac ggattgagtc cggcgatttc tattgatcag aaaacaacaa
    16681 gtaaaaatcc tcggtcgaca gtaggtactg tgacggaaat caatgactat ttacgcttgt
    16741 tgtttgcacg tgtaggacat ccgatttgtc ccaacgatca tatcgagatt accagtcaat
    16801 cagtagatca aatggcagat caagtaatgg ctttgcctga gcgcacaaag atccaagtgt
    16861 tggcaccgat cgtggtaaag aaaaaaggac aacataaaaa aatattcgaa cgaatccaaa
    16921 aagagggata cgtccgcgtt cgagtcgatg gtgagatcta tgatttaagt gaagtgcctg
    16981 agctggagaa aaacaaaaaa catgatatcg cgatcgtcat cgaccggatc attgtgaaag
    17041 aagggattcg ttctcgccta tttgattctt tagaggcggc cctgcgatta gcagacggct
    17101 atgctctggt tgacgtgatc ggtgaagaag aaatgttgtt tagcgaacac tatgcctgtc
    17161 cttattgcgg atttactgtt ggcgaactag aaccaagact gttctcattc aatgcgcctt
    17221 ttggagcttg tccagactgt gatggtttag gtgtcaaatt agaagtcgat caagatttag
    17281 tgattccaga tagaacgaag acacttagtc aaggtgctat cgttccatgg aatccaatca
    17341 gctctcagta ttatcctcaa atgttagcgc aggcttgtga aagtttcggt atcgatttag
    17401 atacaccatt cgaagaatta ccggaagagc atcaggagat catcttgaat gggacagatg
    17461 atcttttcca tttccattat gaaaatgatt ttggtggcgt tcgcgatgta gaaacgactt
    17521 tcgaaggggt attgaagaat atcgagcgtc gttatcatga tacgaacagt gatttcacgc
    17581 gtgagcaaat gcgtctttat atgacagaat taacttgtca gacatgtaaa ggttatcgat
    17641 tgaatcccca agctctatct gttcaaatca atggaacgaa tatcgggcaa acaaatgagt
    17701 tgtctattca aaaagcagta gatttcttct caaacttaac gttatctgaa caagagaaag
    17761 taattgccaa accgatttta aaagaagtaa atgacagact ttcattcttg gaaaatgtgg
    17821 gactagacta tctaactttg agtcgcgctt ccggaacatt atctggggga gaagcgcagc
    17881 gaatcagatt ggctacacaa attggatcga atctttctgg agttttgtat attttagatg
    17941 aaccatcgat cggattgcat caacgcgaca acgatcgact gattggctca ttaaagaaaa
    18001 tgcgtgatct tggcaacacc ctcgttgtag tcgaacatga tgaagatacg atgtgggcag
    18061 cagattattt gattgacgtc ggacctgggg ccggagaaca aggcggacaa atcgtggctg
    18121 caggcactcc tgaagaagta gctaatgatg aaaattcttt gacagggaaa tatctttcag
    18181 ggaaaaaatc aatccctgta ccaaaagaac gccgtaaagg taatgggaaa gcaatcaaga
    18241 tcactggtgc ttcagaaaac aatctgaaaa atattagtgt tgaatttcca ttaggagaat
    18301 ttgtagcagt cacaggtgtg tctggttctg gaaagagtac attggtcaat agtatcttaa
    18361 agaaatcgtt agcgcaaaaa ttaaataaga attctgctaa gccaggtaaa ttcaagacaa
    18421 tttccggcta cgaaagtatc gaaaagatca tcgatatcga tcaaagccca atcggccgga
    18481 cgccgagaag taatccagcg acttatacaa gtgtatttga tgatatccgt gggttatttg
    18541 ctcaaacgaa cgaggcaaaa atgcggggtt ataagaaagg gcgttttagt ttcaacgtaa
    18601 aaggcggtcg ttgtgaagct tgtcgcgggg atggaattat taagatcgaa atgcactttt
    18661 tgcctgatgt ctatgttcct tgtgaagtat gtcatggtaa acgatataat tctgaaacat
    18721 tagaagtgca ttacaaagga aaaagcattg ctgatatttt ggaaatgaca gtagaggatg
    18781 ctgtagaatt cttcaagcac attccaaaga ttcatcgcaa actgcaaacg attgttgatg
    18841 ttggcttagg ttatgtgacg atggggcaac cagcaacgac attgtccggt ggtgaggcac
    18901 aacggatgaa acttgccagt gaattgcaca aaatctctaa tggaaagaat ttctatatac
    18961 tagatgaacc aacgacagga cttcatagcg atgacatcgc ccgcttgttg catgtattac
    19021 aaagattagt agatgctggt aacacagttt tagtgattga acacaatcta gatgtaatca
    19081 aaacagcaga ttatatcatt gatttaggac cagaaggtgg agaaggtgga ggaacgatcc
    19141 ttgcgactgg aacaccagaa gaaatcatta acgtaaaaga aagttataca ggtcactatt
    19201 tgaaaaaaat aatggtataa agaggataga tagctgagat agttaaaaaa acataaaaaa
    19261 atgaattttt gcttgtttta ttaatatcag tgttctagaa tcattacatg gtagttcttt
    19321 ctacactgat tggtatagat aaaaacttcc tataagttca atccgttcaa aaaagaattg
    19381 agcaatcgga ggactttcct ttgattgctt ttttctttat ggtatgatat tccttgtgta
    19441 gttggaaaag aaaaacaaga gattatgagt aagtgagaga taatgatgaa cagtattgaa
    19501 aatgaagaat ttatccctat attattagga agcgacatga acgtttatgg tatggcacgt
    19561 tcttttaatg aagcatacgg caaaatctgc caagcatatg cttcggatca gttagcccct
    19621 acccgctaca gtaaaattgt caatgtagaa gttattccag gatttgataa agaccctgta
    19681 tttatcgaga cgatgcttcg tttggcaaaa gaaagatatt ctgataagag taaaaaatat
    19741 cttttgattg cttgtggtga tggatatgca gaattgatct cccaacataa acaagagctg
    19801 tcagaatatt ttatctgtcc atatattgat tattctttat ttgaaaggct gatcaataaa
    19861 gtaagctttt atgaagtttg tgaagaatat gatcttccgt atcctaaaac attgatcgtc
    19921 agagaagaaa tgttagtaaa tggacatttg gaacaggaat tgccttttga attcccagtt
    19981 gctttgaaac ctgcaaacag tgtggagtat ctttctgtcc aatttgaagg aagaaaaaaa
    20041 gcttttattt tagagactag agaagagttc gatttaattt taggccggat atacgaagcc
    20101 ggttataaaa gtgaaatgat tgtacaagat tttatcccgg gtgatgatag taatatgcgt
    20161 gtgctaaatg cgtatgtaga tgaagatcat caagttcgga tgatgtgcct tggtcatcca
    20221 ctattagaag atcctacacc agcatcgatt ggtaattacg ttgtgatcat gccagattat
    20281 aacgaaaaaa tctatcaaac cattaaggca tttttggaaa aaatcgaata tacaggcttt
    20341 gccaatttcg atatgaagta tgatcctaga gatggagaat ataaattatt tgaaatcaat
    20401 ttgagacaag gtcgtagcag tttctttgtg acattgaacg gcttgaatct ggctcgtttt
    20461 gtcacagaag atcgagtgtt caacaaaccc tttgttgaaa cgacctatgg aacgaatcaa
    20521 tcagataaag caagattatg gatgggtgtg cccaaaaaaa tctttttaga atatgctcgg
    20581 gaaaatgaag acaaaaaact agctgagcaa atgatcaaag aaaatcgtta cggtactact
    20641 gtattttatg aaaaagaccg atcaatcaag cgctggttgt tgatgaaata tatgttccat
    20701 aactatattc caagattcaa aaagtatttt catgtgaaag aaggctagtt catggagaat
    20761 tttttcagta ttttaggcgg aatgggcacg atggcgacag aaagttttgt tcgcttgatc
    20821 aaccaccgaa cgaaagctac aaaagatcaa gaatatttga attatgtctt attcaatcat
    20881 gcaacagttc ctgatcgtac agcttatatt ttagatcgat cagaagaaaa tccaatgccg
    20941 tttctgctgg atgatattga gaaacaaaat ttattgcgac caaacttcat tgtgttgact
    21001 tgtaacaccg cacactactt ttttgaagag ttacaagcgg caacggatat tcctatcttg
    21061 catatgccaa gagaagcagc aaatgaactt gtccgtcagc atacgacagg gagagtagcg
    21121 attctaggaa cagaaggaag tatgaaagct ggaatctatg aacgcgaagt caaaaatctt
    21181 ggtttcgaaa cagtgattcc tgatactgcg cttcaagaaa aaatcaatta cctgatctat
    21241 catgagatca aggaatcgga ttatttgaat caagagctct attacgaaat cttagaggaa
    21301 gcggtagagc gcctaaactg tgaaaaagtc atcctaggtt gcacagaatt atctttgatg
    21361 catgaatttg ctgaagataa tcattaccca gtgatcgatg ctcaatctat tttagcggat
    21421 cgaacgatcg aacgtgcatt agctgaaaga agcgaggcat tggatacagc atcggaaaag
    21481 taaaaaaatt ggatttgggg caaatatcct aaaagatatt tggtccaaat ctttttgtaa
    21541 aagaaaaaga aaactggtaa tgatacgccc gggatgatat aattttatta tgcacaatgt
    21601 gagaggggtt cgtaaatatg acagataatt tgcaattagt catcataaca ggtatgagtg
    21661 gtgcagggaa aacagtagct atccaaagtt ttgaagatat gggctatttt tgtatcgata
    21721 atatgccgcc tcgattgatt ccaaaattct gggaattgat caaagaatca ggtaaagtca
    21781 caaaaatggc tttaggagtg gatttgcgtt ctcgttcctt ctttgaagaa atccaaaaca
    21841 tgctgatcga aatcgaaaat acgaacttca tcgacacttc tatcttattt ttagatgctt
    21901 cagacgaaga actagtggct cgctacaaag aaacaagacg agcacatcca atggcgatgg
    21961 acggtttgat cactgaaggg atacgtaaag aacgggcgat actagacgac ttgaaagccc
    22021 aagcttccgt gatcattgat acgacaacat tgacaccacg acaattacgt gaaaaaatca
    22081 atgagtcatt caaatcaaag ggagaccaag gatttcggat cgaatttgta tcatttggtt
    22141 ttaaatacgg gttgccgatc gattcggata ttgtgatgga tgtgcgtttt ttgcccaacc
    22201 ctcattatat cccggaacta agaccattga ccggaaaaga tcagccagtc tatgattatg
    22261 tgatgtcctt tgatgaaacg caatcattct accataaatt tttggatctt ctggaaactg
    22321 tattgcctgg atacattaaa gaaggaaaaa gcagcttgac agttgcaatc ggctgtacag
    22381 gcggtcaaca ccgttctgtg gcattgactg aacgtttggc aaatgcttta gggaaaaact
    22441 ataaagtcaa tgtgacacat cgggacaagg ataaaaggaa agagacggtg aaccggtcgt
    22501 gaaaatgaaa acctaccgga taagaaaacc aaagatcgta gtggtcggcg gtggcactgg
    22561 tctgccagtt atcctgaaaa gtttacgaaa tcaaggagca gatatcacag cagtagtgac
    22621 tgttgcagat gatggtggaa gcagcggtgc aatcagagaa tcgatcgcta tggctcctcc
    22681 tggtgacttg agaaatgtct tagtggcgtt atcggatatg ccacaattct atgaagatat
    22741 tttccaatac cgtttcaaaa aggaagatca gtttttagcc aatcatacga tcggtaatct
    22801 gatcattgcc gcagtttccg agatgcgggg aagcacttat gaggcgattc aattattagc
    22861 aaaaatgatg catgtagatg gacatgttta tccttcttca gaaactcctt tgaccttgca
    22921 tgctgttttt aaagacggaa cgactgcagt aggagaatct aaaatcgcga tcaaccgtaa
    22981 aaccattgac cgtgtgtatg tctgcaacac aaatgataaa agtgaagcca aagcggctcg
    23041 aaaagtcgtt tcagcaatta tggacgccga catggtcgtt ttgggacccg gaagtttatt
    23101 caccagtatc cttccaaatt tagtgatacc agaaatcggc gaagcaatgc tcaagacaac
    23161 tgctgaaacc gtctatatct gtaatatcat gacccaaaaa ggggaaacgg aacatttcac
    23221 agatgccgat catatacgtg ttttacacaa gcatttgaat cagcctttta tcgatacagt
    23281 attggtcaat acggaaaaag tgcctgaaga ttatatggac ccagagatct atgatgagta
    23341 tctagtacaa gtcgcacacg acttcaacgg attgagaaat gaaggatgtc gagtgatttc
    23401 caccgacttt ttagaattga aagatggtgg tgtcttccac gatggagaca aagtagtaga
    23461 agaattgttc cgtttagttt tcggttcaaa atattaagca atttattgtt ggagaaagga
    23521 ggagaatagg atgtcatttg ctgcagatgt gaagaaagaa ctaactggtt tggaagttca
    23581 tcgagaacat gcgaaagcgg aattagctgc tttgctgcga atgaacggct ctttaggtat
    23641 tgtgaaccag cagtttattt tgaacgtcca aacagaaaat gctgcaattg ccagacgtat
    23701 ctattctctt ttgaaagatc attatcaggt acgaagtgag ctactcgttc gtaaaaaaat
    23761 gaaattaaaa aagaacaatg tctatatcgt ccggttgaag caaggcacaa aagatgtgtt
    23821 gatggatctt gatatcatgg atggtatgat gttcaatgga cacgtttcgg acgagattat
    23881 gggaaattcg caaaaaatgc gttcttactt acgtggtgct tttatggcct caggttcagt
    23941 caataatccg gaaacgagtc gttatcattt ggaaatattt tctatttatg aacagcataa
    24001 ccaagacatt tgcgatatgt taaattatta cggtctacat gctcgaacgc ttgaacggcg
    24061 caatggctat atttcgtatt taaaaggagc agaaaaaatt gctgatttct taacattgat
    24121 tggtgcaacc aactcaatgc tccgatttga agatgttcga atcgtacggg atatgagaaa
    24181 ttcggtcaat cgtctagtca attgtgagac agcaaatctg aacaagacga ttgatgcggc
    24241 ttctaaacaa atcgaaaaca tcgaattgat cgaagcaaag gtcggtctac atgcccttcc
    24301 tgaaaaactt caggaaatcg cagaacttcg gcttcaacat ccagaggtca gcttgaaaga
    24361 attaggggaa atgattccct caggtgcaat atcaaaatcg ggaattaatc accgaattcg
    24421 caaaataaat gaatttgcgg aaaaactgcg tgaagaagca gtttaatcca aagaaaaaac
    24481 tgatacatga agcaggagat gcgaatgtat cagttttttt taaaagctat acttgctttt
    24541 cttccgtcaa actataattc caactttact ggagtaacat aatgttgaaa ggcttctatt
    24601 gttttttaat acaatcgtat aaaaagcggg gagacaactg aatgaagatt ttagttttat
    24661 acataaactt ttgcttaata tagaagtcct gatatagata ggatcaagta tttatctata
    24721 actggagatt aattaaaaac tggaaggaac tgctcgatca accttgagaa ctgtttttct
    24781 tacttctggt caagattctc gtggttggtg gataaggatg gttgataagt tcattggatt
    24841 agctagtcga aaacaaccag gtgtaattaa tgtagaagtt ttaacaaggg gatggagtga
    24901 tttattcagt gataatcata agttcataaa tagtaattgg gatgtatggt tggtatacgt
    24961 tatacctgaa cttgaactat caagatttgg tttagaattt gagaatcgat gtttataaag
    25021 ggagggcaaa aaatgtgctt ttttacaact tttactatca ttatgattgc agtacttttt
    25081 atttttctag atatagcaaa gcgaaataca gcatttttat tatatcgggt tttacttcga
    25141 gcaggtctca taacatttat atcgatcgtt ggattttttc tattcacagt tattgtattt
    25201 atttggagaa ctacagcacc accattgcca gagattacat atggagaatt tccgtttagt
    25261 cttgaatatg aaatagatga ggaaagaatt ttgattgaag atacggtgat tgcagaattt
    25321 ataggaagtt ttagagggaa tgtgacaact caacctagac gaagttggag tataaaactt
    25381 ttaaataata gtggagagtt gaaagcattt cattatgaat tcttaataat agaaagtgaa
    25441 gatgtaacaa tcactttaag tagtggctct gctggatatt ttatgaatga tggtggaagc
    25501 aagccacagc tcacagtccg tgatcgtatt gcaggtttta tgcatttttc tcactatgac
    25561 ttagaagaag ctcgtaaaat acttgcagaa tatgggatca cattaatcaa ttgggaatat
    25621 gcgccaccga taagaaattt tttcaatgat tattattagt cttataaaaa acagaatgta
    25681 aaatttatgt gcgcgagtat ttagttttcc attttttgtt tgatagatat atagcattgt
    25741 tgaagcatgg gatagtggtg ccaattttat caatgaggtt ggtcaggcag ttaacataat
    25801 tggtgatgct ataaatccat ttaattggaa ttggaggaat taggtgtgga aacagtgaga
    25861 ttatggatat tcatgaaaaa ttttcgttcg gctttgagat atgttattct ggaatcaaaa
    25921 tcaggttatt atctgattga cacagatcac tatacttttt ggggaggcta cctttttccg
    25981 atttttaatt ggataatccc ccataaggtt atgaaaatta acttgcctgc agaagaaata
    26041 aatagattaa tactcaacaa agatttacaa aggaaaagag agaaagaaca ttctaatgtt
    26101 gcttggatag gtccagcatt tgcagtagtg acttcttcaa ttgtcggtcc tatgctagag
    26161 agtatttata gtgatttttc tattggaata cgttatttat ttgttacttt aagtatagct
    26221 gttcttttgt gcataaaggg ttatgttagc aagaagaata aagatatttt aacactaatc
    26281 ggtgtaaaca atttatctag tactaaaatt atactgtttc ctgaatctat aaaagaagga
    26341 tcaaggttgt tgataatttt tataatgctt atactgtttt ctgttttatt tggggtgatg
    26401 tttataga
//
LOCUS       DAZULO010000028        26159 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000028 DAZULO010000000
VERSION     DAZULO010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.029"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..1092)
                     /gene="queA"
                     /locus_tag="ACG8OA_001193"
     CDS             complement(61..1092)
                     /gene="queA"
                     /locus_tag="ACG8OA_001193"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFS4976017.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1285..2460
                     /locus_tag="ACG8OA_001194"
     CDS             1285..2460
                     /locus_tag="ACG8OA_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4976018.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2464..3105
                     /locus_tag="ACG8OA_001195"
     CDS             2464..3105
                     /locus_tag="ACG8OA_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4976019.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3102..4019
                     /locus_tag="ACG8OA_001196"
     CDS             3102..4019
                     /locus_tag="ACG8OA_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4976020.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4016..4687
                     /locus_tag="ACG8OA_001197"
     CDS             4016..4687
                     /locus_tag="ACG8OA_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4976021.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5228..5716)
                     /locus_tag="ACG8OA_001198"
     CDS             complement(5228..5716)
                     /locus_tag="ACG8OA_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFS4976022.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5917..6183)
                     /locus_tag="ACG8OA_001199"
     CDS             complement(5917..6183)
                     /locus_tag="ACG8OA_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS4976023.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6183..6446)
                     /locus_tag="ACG8OA_001200"
     CDS             complement(6183..6446)
                     /locus_tag="ACG8OA_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4976024.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            6967..7107
                     /locus_tag="ACG8OA_001201"
                     /pseudo
     CDS             6967..7107
                     /locus_tag="ACG8OA_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(7249..9072)
                     /locus_tag="ACG8OA_001202"
     CDS             complement(7249..9072)
                     /locus_tag="ACG8OA_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4976025.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9670..12375)
                     /locus_tag="ACG8OA_001203"
     CDS             complement(9670..12375)
                     /locus_tag="ACG8OA_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFS4976026.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(12681..13628)
                     /locus_tag="ACG8OA_001204"
     CDS             complement(12681..13628)
                     /locus_tag="ACG8OA_001204"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFS4976027.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            13818..14087
                     /gene="rpsN"
                     /locus_tag="ACG8OA_001205"
     CDS             13818..14087
                     /gene="rpsN"
                     /locus_tag="ACG8OA_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS4976028.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(14263..14625)
                     /locus_tag="ACG8OA_001206"
     CDS             complement(14263..14625)
                     /locus_tag="ACG8OA_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4976029.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(14636..15757)
                     /gene="alr"
                     /locus_tag="ACG8OA_001207"
     CDS             complement(14636..15757)
                     /gene="alr"
                     /locus_tag="ACG8OA_001207"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFS4976030.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(15773..16123)
                     /gene="acpS"
                     /locus_tag="ACG8OA_001208"
     CDS             complement(15773..16123)
                     /gene="acpS"
                     /locus_tag="ACG8OA_001208"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFS4976031.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(16253..17764)
                     /locus_tag="ACG8OA_001209"
     CDS             complement(16253..17764)
                     /locus_tag="ACG8OA_001209"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4976032.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(18086..19438)
                     /gene="murF"
                     /locus_tag="ACG8OA_001210"
     CDS             complement(18086..19438)
                     /gene="murF"
                     /locus_tag="ACG8OA_001210"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFS4976033.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(19591..20667)
                     /locus_tag="ACG8OA_001211"
     CDS             complement(19591..20667)
                     /locus_tag="ACG8OA_001211"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFS4976034.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            20863..22608
                     /locus_tag="ACG8OA_001212"
     CDS             20863..22608
                     /locus_tag="ACG8OA_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFS4976035.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(22888..23325)
                     /locus_tag="ACG8OA_001213"
     CDS             complement(22888..23325)
                     /locus_tag="ACG8OA_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFS4976036.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(23309..24682)
                     /locus_tag="ACG8OA_001214"
     CDS             complement(23309..24682)
                     /locus_tag="ACG8OA_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976037.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(25004..25492)
                     /locus_tag="ACG8OA_001215"
     CDS             complement(25004..25492)
                     /locus_tag="ACG8OA_001215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4976038.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(25514..26116)
                     /locus_tag="ACG8OA_001216"
     CDS             complement(25514..26116)
                     /locus_tag="ACG8OA_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS4976039.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         7506 a         5559 c         4836 g         8258 t
ORIGIN      
        1 tttgtcataa aatctttgta aagctttttt gttattcaaa actagcggct tttcataacc
       61 ttatttcacg aacatcgcat ctccaaaact aaagaaacga tagcgctctt caatcgcatg
      121 ctggtaagca gataaagtca attctcttcc tgcaaaggca ctaactaaca tgactaaagt
      181 cgattttggc aaatggaaat tagttgagaa agcttctacc actttgaatt catagccagg
      241 cgtaatgaaa atatcagtcc atccgctgtc tgctttgatt tctccattaa atttcgttcc
      301 aatcgtttct aatgttcgaa ttgaagttgt tccgacagcc acgattttcc cgccattttt
      361 acgtacttgg ttcaatcgct cagccgcttc ttccgtcaaa cggtagaatt cactatgcat
      421 atggtgttct tcaattttat ctacgctgac aggtcggaat gtacctaacc ctacatgtag
      481 tgtcagatag actaattcaa caccctttgc ttcgatctca tcaagtaatt ctttcgtaaa
      541 atgcaatcca gctgttggcg ctgcagcaga accattttct ttcgcataga cagtttgata
      601 gcgatctggg tcttccagac gttctttgat atatggaggc aatggcattt ctcccaatga
      661 ttcaagaatc tctagaaaaa ttccttcata ctcaaagtca atgatccgtc cgccgtgttc
      721 caattcttct ttcactactg cttgcaatct gccatcacca aaagagattc ttgtcccaac
      781 tttggcacgt ttcgcaggtt tgatcaatgt ctcccacgta tctccttgtg tattcgttag
      841 aagaaggact tctaaatgcg caccagtatc tggtttttct ccatataaac gagcaggcag
      901 aactcgtgta ttattcataa ctagcgcatc accaggctgt aactcttcaa tgatttcatg
      961 gaaatgttta tcttccatct ctccagtttt ccgatccaaa acaagtaaac gagaatgatc
     1021 gcggtctttt aatggtgttt gtgcgattaa ttcttccggc aagtcaaaat caaaatcttc
     1081 tgttgttaac atatcgtcac tcactttcta tcatgtgctt catcattcta tcatgttttt
     1141 cgttctgttg gcaaaatcag cactttttca cttctatttt ttgttttcct aaataaaaaa
     1201 actaccctat ttgtattttt tcattgtttt aggcatacta gtaatagata gttcttcatt
     1261 gaaaatagaa aaagaggtgg caccatgatt gaatttcaaa atgtgtcaaa aatttataaa
     1321 ggtggaaaag tcgctgtaga ggacgtcaat ctttcatttg aaaagggtga gttcatttgt
     1381 tttatcggta cgagcggaag cggaaaaacg acgacgatgc ggatgatcaa ccgaatgact
     1441 gatccaacac aagggaaaat tttgattgat ggaaaatcca tccaagaaat tgatccagtg
     1501 gaattgcgac gtcagatcgg ctatgttatc caaagtattg gattgatgcc tcacatgacg
     1561 atccgtgaaa atatcactct tgtccaaaaa ttgttgaaag tcagtcaaga agaacggaat
     1621 aaaacagctg aaaaaatgat tgatcttgtg gaattgcccc gtgagatgtt agatcgctac
     1681 ccacatgaat tatctggtgg tcagcagcag cgtattggag tcgttcgtgc ccttgcagcg
     1741 aatcaagata tcatcttgat ggacgagcca ttcggtgcgc ttgatccaat cactcgtgat
     1801 tccttgcaag accttgtcaa agatttacaa gagcgtttgg gaaaaacgat cgtatttgta
     1861 acccatgaca tggatgaagc gattaaacta gcaagccgta tcgccatcat gagtgaaggc
     1921 cgtgtgatcc aatttgatac accagaaaat attttacgtc atcctgcaaa cgatttcgta
     1981 gaagagttga ttggagaaga tcgcctacta caagccaagc caaatgcgac aagagtcggc
     2041 gaagttatgc taaatacagc aatcacgatt acaccggaaa aatcattaca agaagcaatc
     2101 aaactgatga gggaaaaacg tgtagatacg ttgttagtta ccgataattc gaatgtatta
     2161 aaaggattta tcgatgtaga aacgttgaat aaaaaacgag gaaaagtatc cagtgtcggc
     2221 gacattttga ataaacatgt tttttacgta aaagaatccg cctatcttcg tgataccttg
     2281 caacgcattt taaaacgcgg gctgaaatac gtgccggtcg ttgatgaaca gaacaaagtc
     2341 gtaggtatcc tgactcgtgc atcattagtg gatatcgtct acgatgtgat ttggggagaa
     2401 gatcccgcaa ctactgatgt tgcagagtca gctactcctg aagaatctaa ggaggtctga
     2461 cctatgcagc agttttttca tcagcacggt tcagaaattc tctcaaaagg ttttgaacat
     2521 ctttatattt caggacttgc tttgatcatt ggtatcatca ttgctgtacc gcttggtgtc
     2581 ttactgacac gttttccaaa agctgccacg attgtgattg gcttaaccag tgccttgcag
     2641 acaatccctt ctcttgcttt attggcattg atgatcccgc tatttggcgt aggtaaatta
     2701 cctgcgatta tcgcactctt tatttattca ttattgccga ttttacgtaa tacgtacatt
     2761 gggatgaaaa atgtcgattg gaattatcgg gatgtggcaa aaggcatggg gatgactgag
     2821 ttccaatcca ttttttccgt tgaattgccc attgctatgc caacgatcat ggctggtatt
     2881 cgtttagctg ccgtttacgt gattgcatgg gctacacttg cctcttatat cggcgctggt
     2941 ggtttaggcg atttgatttt tagtggactg aataattacc aaccagatct cattttggca
     3001 ggtacgatcc ctgtaacaat tttagcacta ttggctgatt ggcttctagg tctcttggaa
     3061 caccggctga caccgatcgc acttagagag gagaatgaag aatgaaacgt atactaaaaa
     3121 tgaccatggt tcttttctct cttcttatac ttgcgagttg ttccttccct ggtcttgcca
     3181 gcaatacaga tgaagacacc atttcgatca caggtggaat cacttcagaa gcacagatct
     3241 tagcaagtct agttgccgga atggttgaac attatacaga taaaaatacc gcaatcatca
     3301 acaatctggc tactacaact atcaaccatc aagcgatgat gaacggagat gcttcaattt
     3361 cagcggctcg ctacacagga acagatttaa cgactacatt gaaccttcct cctgaaaaag
     3421 atccgaaaaa agcgtttgct accgtgaaag atgaatttga gaaaagatac ggtcaaacgt
     3481 ggttcccgtc ctatagtttt gaaaatacgt atgtctttct tgttcgaaaa gacaccgcac
     3541 aaaaatacca tctatctaaa gtcagcgatc tgaaaaatgt agcggatgaa ttagtagctg
     3601 gtgtagatac ttcttggatc aatcgtaaag gagacggtta tgacggcttc caagaaactt
     3661 atggcttctc cttcaattcg atattaccta tgcagattgg acttgtttat gacgcagtcg
     3721 aagcaggaaa aatggatatt gttcttggct actctacgga tggacggatc gccagttatg
     3781 atctagtcat gttggaagat gatcgtcaat tcttcccacc atatgatgct gctccagttg
     3841 tagacaacaa gatattagct gatacgcctc atttaaaaga agccttaaat aagttaggtc
     3901 ataccatcag tacggaaaaa atgcaggaat tgaactacga agcagataat aatctagtgg
     3961 aaccatcagt agtagcacaa cgtttcttac aagaaaacca ctattttgaa gggaagtgaa
     4021 gcaattggat aaaatgaatg tctttcaaca atttttctat tatttccagg aaaatggctc
     4081 ctatatcttt gcacaattta tccgtcactt cctgatttct atctatggtg tcctctttgc
     4141 agcagttgtc gggattccgg ttgggatcat gatttctagg agacgtaaat tagcaaactg
     4201 ggtgattcgt ttggcgaata tcatccagac gatcccttct ctagccatga tctccatttt
     4261 aatcattgga ttaggcctgg gagtcaatgt cgtgattgtt actgtatttt tgtattccct
     4321 cttaccgatc atcaagaata cgtataccgg gatgatccaa gtagacaaaa acatccttga
     4381 tgtcggcaaa ggcatgggga tgactgctag acaacgacta ttcatggtag agttaccact
     4441 ctctgtttct gttatcatgg caggcataag aaacgcgtta gttgttgcca tcgggataac
     4501 cgcgatcggt gcattcgtcg gtgctggtgg tcttggagat atcattatcc gaggaaccaa
     4561 tgcaacagag ggcacatcga ttattttagc aggtgcattg ccaactgcat taatggcgat
     4621 catcacggac tggcttttag gtattttgga aagacggcta gatccagcca gccgtacatc
     4681 acgctgatgt tattgtaaaa aacaaaagct tctgcgacca tttgttggtc gcaaaggctt
     4741 ttttagttct gtcattttta aggcaataat ccactctgat tcttccctcc ttatgggtaa
     4801 agagttgctt ttcttagcta tttatccatt ccgtatactc caacttcata ctgccaacgg
     4861 gttcatatct gcatccaccg actaagtttt tttcttatcc gctaaggcat tttgccaatc
     4921 cattttccca tcactcgtca tggaattcat accacatgat ctctaaatac tcgtctgctt
     4981 gttctttgaa ttgggcatca attcttacat cctttggcgt ggctttcatt taagcctttt
     5041 ccatcagtcc tcgattgttc gatgccttca gctgcatcat caaaactggt gactgccgta
     5101 tcggtaactt cgatcatctt ttatacaatc gggaacttgc catcctcatc cttggctaaa
     5161 aacatatcta gccattccag cttctcgcca tgggaggttt gcggttccta aaaccggtac
     5221 acgattctta tggactgcat ccgtcgcaac tagacttggc gatacgatga tccttcacca
     5281 gaagagcacc cccgtaaacc aattgatcga tatattgcca ataagaaaag acattggcgt
     5341 aaaagatatc gatccctcgt gatgcattgc cgttgatact gctattcatg gccgaaagcg
     5401 ccacgacttt aacttcttcc gtttgagtga cattcatgtt cggcaatgtc gctttggata
     5461 cccgctctgc taaaggaacg acactggtat tgtatttcgt atctgtatct tttccaaaag
     5521 accatgccaa cgaatcctct gggaaccaat aagacgactc cggttgattg tccattgctt
     5581 ttttcaacat acttttggct gtttctcgat aaacgacatt tccaattgtc ttcgcctcac
     5641 tttggcgata atcgatggtc gttgccactg ccaacactaa caacgatgct ccaattttac
     5701 tgattttcct tgtcacactt cttcctcctt gttttacata aacccgtcaa cattccattc
     5761 ctaaactcaa acaatgaccc ttcaaaggtg gagatatagg aacaaattaa agaaatcatg
     5821 caataaatcc aagaaggttg gttttagaac aaaaaaagag tctccttttt ggggagactc
     5881 tttgttaact atctaaaaag gttggctctt ctcacttcac tcataatgtg tccaggcaga
     5941 ataaatttct acaactttat tttcctcatc aactttgtaa accactcgat gctgtctatt
     6001 cagcctgcgt gagtatctgc cttcatgtgt aggtctcagc ttttcaaaag actgcgtggg
     6061 catatatgga tcttccttca atgtctgaat cacttcttca aattgtttct ttaaattcgt
     6121 ttgcttgatt tttcttaaat ccactttcgc agagtttttg attgcgactg tatagttact
     6181 catcaaagag cttcccaatc aatatcatcg atattcgtaa agctactgtc gtctttttcg
     6241 cggtctcgta ctttatctaa agtacctgtt tgctcaagaa aaagggtttc ttgaatcgct
     6301 ctccagtctt ccagcccaat caatacagca ttgtttccac tacgatcact aataatctcg
     6361 atttcagtat gattttcatt tacctctttc aataactgat aaaaattttt acgagcagtg
     6421 ctaggattca tcgttgtatt agccattgtc tctcctcctt tttctgatat aattatagcg
     6481 tacttgatta agtacgtcaa aaaaatacaa taaagcactt aaattcagca aacttttcct
     6541 cttttttaca tagagaaaaa gacccctttt tagggaggat ctttgtccac gctcacttct
     6601 tactccttta cgactagcgg tagccaaacg taaagtaatt ggtaacaacg tatcatcttt
     6661 agcaaaggtc gaatccaaag cctgtgagtc tatgcaacaa caccaataca cgaaggttcc
     6721 tcgttgcagt gtgacttgat tgatatcttg aataacctga ggatacgcac gaaagatttg
     6781 taacggttat tcaaaactga ttttggccgt caagtcttct actgcctcaa aaatgattgc
     6841 ctctttcgtc tcctgtcatt catgttcact atcgatttga tacttgctta gaccgttggg
     6901 tttgtgaatc accaattaaa aatggcactg agttatggta acaaaaaatg gtcacttgac
     6961 gaggctgaga ttcctactta tgaagacaca tggcacttaa gagtcgaggt aaaggaagaa
     7021 gctgaaaata taggaggctg tacattattt ggcaaaggat tcggaaatta tgatcaagac
     7081 atcaaaatca aaatgtttta cagctaaata ggaagaataa taacatttca tacgctgttc
     7141 ccatctttaa tggcttatag agggcacctt tagctgagaa taagacaaaa cagaccctct
     7201 agcttcaatt aagagctaga gggtctgttt ttgattttaa aacttggttt atttcttcaa
     7261 atggtagcta taggtgctga caacgatatt ttcacgccaa gacaatcgca atcttaaccg
     7321 caaacttgtt tggttatgga gaatattctg ccagcgtctg tttggtacaa attgaggaat
     7381 caacacagta gttgtatagt tttgtttcgc tgcattttta cttacgatat ctacataacg
     7441 gataattgga ttttcaatcg agcgataaga agagtggacg attgaaaaac ggacatctgg
     7501 gaaatgcttt ttaaattctg cttcgatttc tttttctttt tcgacatctt cgtctagcga
     7561 aacatgcatc gcaacgacat aatcaccgat cgaacgtgcg taattcaacg caccgatatt
     7621 tacccgtgtc acattcccga ctaatacaag aacggtattc ccgtcataat catgtagttt
     7681 tgcatcattt tctaaacgaa gctgttctgc tactttttta taatgatcat ggatcttata
     7741 aaaaatgaac ataacgattg gcataatgat gaagaaaggc caaatgtccc ctaaccgata
     7801 gacaaacaaa atcgcaatga ttgcatatga gatgaacgca cccgtaatat tggcaatcga
     7861 tttgcttaac catctttttc cttctttttt ccatttgacg accatccctg tttgagataa
     7921 agcaaatgga atgaacacgc cgatagagta taacggaatc aagcgttctg ttgagccatg
     7981 gaaaataaac agcaatacga tcgaacctag cgctagtgtg ataatcccat tggaatagcc
     8041 tagtcgatcc ccacgatctt gatacatgtg aggcatgaat ttatcttttg ccagattgta
     8101 tgcaagaact ggaaaagcag aaaaaccggt gttagcagca actgctaaaa tcagcgctgt
     8161 agcaaattga aggacgtaat acaaaatacc atgtccaaag acagctttac caatttgaga
     8221 caaaacagtg acttcttttt caggaacgat cccataccaa tagttgataa atgtgatccc
     8281 cacgaagaag aaacctaaga tcaatgccat catcgtcaat gtagcagcgg cattttttgc
     8341 tcttggcttc ttaaagaatg gtacagcatt actaatcgcc tccaccccag ttaaagagga
     8401 agaaccagaa gaaaatgctc gtaaaatcaa agcgatagaa acacccggca cagccgctcc
     8461 tggaagtgca gtagcttgca atggcacagc acctgtcacg atattatata aaccaacaac
     8521 gatcagtaac gagatcacaa ggataaatgt gtacaccgga aataatagga acgatgcaga
     8581 ttccctcagc ccacgaagat tcagcatcat cagtagcaaa acgattgtca cagaaatagc
     8641 gacttgatga ccgtagagtg ctggcaccgc tgatgtgatc gcttcagcac ctgctgagac
     8701 agatactgct actgtcaaca tgtagtcgat caataaagag ccaccggaaa tcaagccagc
     8761 attctttcca agattctcac tgctgacgac atatgctccc ccgccatgag gataagcatg
     8821 gatgatttga cgatatgata gcgttaatga aatcaataaa ataataacaa aagcggcaat
     8881 gggtagcgaa taccagatgg ctgcagcaga caaagcgact aaaacgacaa cgatttgttc
     8941 cgtaccatat gcaatagatg ataacgcatc agacgacaat aatgccaaag cagcaaatcg
     9001 ggttaatttg tgctcatcat tttcagcaga ttttaaaggc ttacctacta acaacctttt
     9061 taagtaatcc aatttctaaa ctcctttaca cacaatttta cttcaagttt tatagtcgaa
     9121 aatttatagt aaacaagatg ttatagtacg ctcactgacc ttaaaagtca ataaacttta
     9181 agaaaataat tggaaaaaaa cagaaaaata tcaagcgctt tttcaaacgc tttaatacag
     9241 caaaaatcta acattttttt atgggctttt tctttgaatt ctataataaa aggatagaac
     9301 ttgacttaga aaatcaagtt ctatcctttt tgatatctta acttttggaa gagagatgta
     9361 aggctgcttt ctacccatca ttcttactac tcgaagcata gcgcagttgt cacaacctct
     9421 tgataaacgg tgtttaaaag cttgcatctg cggtaataag cccaaccaaa ttaaaaacat
     9481 aagaagctgt ttttatttgg tcgttcttat tacttgatgc agagcagctt ttttacaacc
     9541 tcttggtaca cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa acggcaaaat
     9601 atgaggaact atttttccat ttttttctct aatactcagg tcaaaacact gatttcacaa
     9661 cctctttttt taatgaaaaa tatggctctc atgcgccgct tctttgaatt tcgtcatttt
     9721 ttcttcgatg aacggataga ttgccgttcc tacgattcca aagacaacaa acaaaccgat
     9781 catgatccag attgctttcg tagcatttgg ccaataaatt cctcctgcag attcccgtaa
     9841 caagtttacc gcatgagtaa atggcaagaa cggattgatc atttggaaga atttccctga
     9901 gacttggatc ggatagttcc cgcctccgcc agagatcgac agtactaaga tgataatagc
     9961 gatcccttta ccgacattat cgaataatgc agccaagaca tagacgatca tcatgaacgc
    10021 taaggcgatc aagacagcaa atgacacgct gtagaccgga ttttggacat ctactcctag
    10081 taagaaaata ttccctaatg taacgatcaa gctttgcgca atcgccattg tcaaaaatgt
    10141 cagcatacga gcaacgaatt gttctcgttt cgtaaaattg tttcggtctt tctttcctaa
    10201 atagtattct gtcgtagcaa cacttgaaag aagaacagcg ccgacccaca gacatagtgc
    10261 agtataaaac ggggtgctag ctgaaccgtt atttgcgatt ggataaagcg cattggtttg
    10321 tagctcaaca ggttgtgtaa agaagtcact ttcttttgtt gcgtctagtt tcaataattt
    10381 gatgacttcg cctagatcga cttcttgttc gccttttcgg atcgcgtctg ctgctttttg
    10441 gataccagct ttgatatttg gccagtcatt gttgatcaag tccgcagcag cgttcacagc
    10501 tgtttcgact tctggcattt tttcatttac tgttgtcaat gtaccggtga tttcattttt
    10561 tagtgctggc caatcatttt ggataaaatc agcggccaat cctaattttt gctgaatgat
    10621 cggtaattca ttgttgtata ggtctgcccc ttcattgatg ccattgacga tggtatccat
    10681 atttccattc agcattgtat tggcatcatg gatttcttgt ccaattgcag gcatttcttt
    10741 ttgatatttt tctaaaattg ccactgcatt cgttacagtt gctttcgttg agctgagaag
    10801 cgaatcaaag tcgatctggt ccgctttgct cagtgcgcct tgtgcagtcc caatcgtatc
    10861 gatgacttgg tctagtagag aagtgacagt ggaactgatt tgacctgtat tgataccagc
    10921 aatgatgcca ttgatattat taacaacggc atcaatttga tctagcaatt catcaatagc
    10981 cttcgtgtct tcattttgaa cagctgtgtt caaagcagat aggcggtttt gcagattgct
    11041 tagcaagggt tttaattgat ttagctgatc aataattggc tgtaatttcg tatttccagc
    11101 acttgtttgc aatttagtca ggaactcgac cagtttatcg attgcatttt gttgttgaga
    11161 tattccgtca ataaagccgt tcaatacatt gctgatgttt ttcttttcat catcagtcag
    11221 cttaccatct tcaatagctg ttcttacagt atctattaac gaacctgtcg ttgttgccac
    11281 ttgtccaatt gacttcaatg taacatctac actgtttgtg atgcttggta tcgcatcttt
    11341 cagctgagag gcacctgatt ttgtttggtt tgctagatca cttgcctgat tttctaaatt
    11401 tttcacatct ggcaagatat cttgtacttg ttggatgatc gtcaagcctt gttttgcttc
    11461 atcgattcct tggttcattg tctcttggat cgaagcaaaa tcgccgtcga tctctgctag
    11521 ttgcttaccg gcattttgga tctctgggat tttttcttgc aaatcaagga tgatttttgc
    11581 ttgatctttc aactcaggca ttttgtcatt caatgcaacg actttttcac ccatctcatc
    11641 tactttaggg atgtaatcca cgaattcatt tgctttattc agtttttcct tgatttcagg
    11701 tagttgactt tgtaattcga ggacttgttt tgtgtagccg tcgatcgtgt ccaaattttc
    11761 atctgttgat aaaatcatat ctttaacttt attgatactt actaggttcg tatcgatgtc
    11821 gtaacctatc tcattgaata ctttcaataa agtcgaactg gctgttttga taaattcatt
    11881 tgtgatttgc gattggatcg aacttgctcc tttatcggtg attttcggtg caatcgcatt
    11941 gattttttca tttaccgtat attcgatttt tggtttcttg atatcaccac tagtaaaact
    12001 cagcagatct tctgagaaat cctttggcaa gtaaatacct gcgtaatact ttccggaacg
    12061 gactccgtct tctaattgtt cttttgaatc aacgaattgc catcctaatt gtttattttt
    12121 atgaagcgat tcgatgactt gctccccaat ggccacttct ttaccttgaa actctgctgg
    12181 cttgtctgca ctgtaaacag caattggcag ctctccagta tttccatatg gatcccataa
    12241 tgctttgatg ttaaaccacg catatagcga agggatgatc atgatcgcga tgatcagaag
    12301 tgttgcaatc gggtttttga aaatccgttt ccagtcaagc aaatataatc gaagcgtatt
    12361 tttgattgac tgcatagttc tcccccttct tcttttttca gctgaattac tttaccatgt
    12421 atttcatact tttttcaatc ggcttttttc aaaaaagtcg aatttttgga catatcacgg
    12481 caattgtctc attgtaatct cttacatatt gagaaataat taaggattac agcctttact
    12541 ttatcatgaa ataaaaggga aagataattt tatgccaaag tttttatcta ttatacgtat
    12601 acgcaaaaaa gctaaaacat tttttgtctt tgcccctaca aaaatgtttc agcttttttc
    12661 gttttttatt cctgtatttc ttatttttcg aatgacagga tgattttccc agcaggagga
    12721 tgtccttcaa ctagctgatg tgcttggatg acatgcttaa gtgaagcaga cagattcatt
    12781 gaaataaaaa catgaaaggc tcctttttgg taggctttga ttatgccagc aaaggcttct
    12841 gcatctagat attcctttct tggaacaaac gattcataaa agccttcctt tttttgccgc
    12901 aaatctagat ctgggaggtc atttaatgca acatacctgc cgcctggttt catgatctgg
    12961 atgccggttt ctcctttgat acttcctttc gtcgcatcga ttacaaggtc tgcctgatca
    13021 gcaaattgca gacctggatt tgttttatca taagcagcaa atgctgaagc tcctaatttt
    13081 tttacctttt cctcattttt actggatgca ctggttaaga ttcggattcc cttttcatgg
    13141 agaagctgga tcagacttga cccgaccgca cctgatgctc ctagtatcat tacagtatct
    13201 gtcggctgga tttccgtcaa atggttgatt agattataag ccgtaattcc tggcgtgacc
    13261 attccggctg cttcttccca actcatttta tctggaattt ttgctacttt tgctgatggc
    13321 aacacgactt cttctccgta agtcccgccg actgcatgga cagctactcg atcacctaca
    13381 tgaacagttg tgacatctga accaatctct gtcacaatac cagcgccatc attacctggt
    13441 acataaggga atttcaatgt tcgtatctct ttcatttctc ctaaacgcaa ggcaacatca
    13501 taaggattca cactgaatgc ttttatttct acacgaacat gtccagaacc gacttctctg
    13561 ggatgagcat cgatttcttc aaaaacatct tgtgcttctc cgtaaccgtt aattgcaaat
    13621 cttcgcatgg tcaccatctc cctttttctt taagtttacg tttatgcctt tattttgaca
    13681 aaaaaaatgt cttttcgttt tggcgcatga ttcaaaacaa aatagttgtc ttttttcgcg
    13741 taacgtgtta aattatttat ctaaaagcgt aaagattacg atttacgcta ataaaaaata
    13801 attggaggaa tttgttgatg gctaaaaaat caaagattgc caaagcaaaa aaacagcaag
    13861 aaacgattga acgctatgct gctttacgcc aggaattaaa agcgcaaaaa gattacgaag
    13921 gcttgcgtaa acttccttta gatgctcgtc cagaacgatt gaaaaatcgt gatttaatcg
    13981 atggacgccc tcgcggatac atgcgtaaat tcgggatgtc ccgtataaaa ttccgtgaat
    14041 tagcacataa aggtttgatt ccaggagtaa aaaaagccag ctggtaatcc aagtctggct
    14101 gtaatgtaaa ggaggagggc gcatggacca ctccttggat catcaaaaaa gatacgttcg
    14161 cttcttctag cagaaatgct ggtactagaa gcagacgtat ctttttttac gcacagctgg
    14221 tcctttttta tatgtaaagg ctgcatggtt acttcgctat atttatttag taacaggagt
    14281 cagtgcttcg atacccacac taattttcaa tgctcggtca acttctgcca taatgggtcg
    14341 atccaaatgg cagacctttt ctttcaatct tgatttatcg atcgttctga tctgctccaa
    14401 caggataacg gagtctcttt ctattcctgt tccagtagct ttgatcccaa tgtgagtcgg
    14461 aagcttaggt ttagccattt ttgccgttat ggccgcaaca atgatcgtcg ggctaaaatg
    14521 gttacctaga ttattttgaa taaccaaaac tgggcgtgtt ccgccttgct ccgatcccac
    14581 gactggagaa agatcagcaa aataaatatc tcctcttttt accataggat cctccttatt
    14641 cttgatattc tcttggtatc cgctgtccta atccgcaagc cacttcatag tggatcgtac
    14701 ctaattgatc tgctatatcc tgcatagtga tttctttgtc tgcatttttg ccgataagcg
    14761 tcacttttgt tccgacagga aattcttgag gcaatcggat catcagttga tccatacata
    14821 cacgcccaat cgtttcacag tagttccctt ctaccaatag agaaaaaccc tgcatctttc
    14881 gtggccagcc atctgcataa ccgatcggta tcgttccgat ccactctgct tcaggagtga
    14941 tatatgtttc tccatagccg attccttcac ctgctgggag ttttttgact tgaatcaatt
    15001 cagaaaccag ctcaagtgct ggctgcaatg gataaacttc cggtagtgcg tgcccagagg
    15061 gattaagtcc gtacattgct actccataac gaatcatatt gccgattgtt tcatcatgcc
    15121 ataaagccgt tgcactattg ctgacatgta catatcgagg aagttcggga agttttttca
    15181 gcacttctat aaagcgttct ttttgcagat tccaatatgt atcatctgcc tgatctgctg
    15241 tagaaaaatg ggtaaataaa ccttcccatt ctaatacacg actttctttg atgaatgcag
    15301 ctgcctcttt tacttcttca ggtgtacaaa atccgatacg acccatccca gtgtctgctt
    15361 ttagatgaat cgaaagaggc gtctctagct tacttttctc taaaacagca gctgctgctt
    15421 gcaaccattc ccgtgtacct gcagttactg ataaatcatg agcaacagct acagtcagtg
    15481 cgtcgtaagg aaccatattc aagataagga tgggctgagt aaatccagct tctctcaact
    15541 cgactccttc atctaaattt gaaacacaaa agcccgaagc tccacctgca actgctgcct
    15601 cagctgtttc aatagctcca tgaccgtatc cgttcgcttt tactactgca aacaattctt
    15661 ttccttgcgg aagtcttgca acttcatttg ctacattttc agttatagct tttttatgta
    15721 tcactgcttt cgttgggcgg tgccaagcga caaccatcgt ttttccttct ttctagtctt
    15781 ccaaaataat ttgtgcaaaa gctgtttcat ttgtatgagt aatactgacc catacattgc
    15841 cttcatgagg tgaacgcgtc acaacaggct gaccattttc attttttaaa atctcgacat
    15901 cttgaaaggt aacttttccg atcccagttc cccaagcttt agagaaagcc tctttgcaag
    15961 cgtaacgccc gcctaaaaat tctacttggc gatgaaaagg caaggactgg aatagtttca
    16021 tttcatcaga ggtcaagatt cgtgcaatga attttggttt ttcttcgatc agccgagtga
    16081 tcctaggaag ttcgactgca tcgatcccaa ttccttttat catatgcttt tctcttttca
    16141 ataaatttag aacactctca ttctaccaaa ttataaccat tttgccacaa ataagtccac
    16201 aaataacaaa aatgaaagtt tctcttaaaa aagagaaact ttcattttat gtttaatcgt
    16261 tatttgaacg gattacaaat ccgcgttttt tatcattgtg acgtttaccg ccatcttttt
    16321 ggctgttatt atttttgcta taaccgcctt ttgatttgtt tccacgataa ttaccgccgt
    16381 cacgacgatt acggttgttt ccgccaccac gtttgccatt tttattgtat ccttttttct
    16441 gcattggcaa tgggcgttct ggtgtgatct tcactggcac tgcatcagct ggatcttttg
    16501 ctgtcgtttt tagcaaaagt gcaactaaat cttgtgcaga atattcttct aataatttat
    16561 ctgctgtttg caggtattta tctagaccat tttctgcaag ttttgtttcg atttgttcaa
    16621 ctgcagcacc taattgacct ttgaatgctt ctttttcggt tggtggacgt aaagtcgtca
    16681 tccgtttttt cgtcaagttt tcaatcacat gcaagtagtc catttcattt ggtgtaacga
    16741 aagtcacaga cataccgcct tttccggcac ggcctgtacg tccgatacgg tgaacgtagc
    16801 tttctggatc ttgagggata tcatagttgt acacatgagt gacacctgaa atatctaatc
    16861 cacgtgcagc aacgtctgtc gctacaagga tatctaaatg accgctctta aatgaacgca
    16921 atacgctcat tcgtttttgt tgagacaaat cgccatggat tccttctgca cggtaaccgc
    16981 gtgcttctaa tccgcgtgcc aattcgtcta cacggcgttt tgtacgtccg aagacgattg
    17041 ttaattcagg tgtttgaaca tcaaacaagc gagtcatgat atcgaatttt tcgtattctt
    17101 ttgcgcggac atagtattga tcgatcaagt cagctgtcat ttctttcgct ttgatcttca
    17161 catgatgtgg atttttcatg aatttaacgc caatattctt gatagctggc ggcatcgttg
    17221 ctgagaaaag taatgtttgt ctttgatcag gaacttgtga aatgattttt tcgatatctt
    17281 ccaagaagcc catattcaac atttcatctg cttcatccaa aactaatgtt tgaacggttc
    17341 caagtttcaa tgtgtggcgg ttaatgtggt caagcatacg tcctggtgta cccacaacga
    17401 tatgtggacg atctttcaag ccacggatct gacggccaat atctgctccg ccgtaaacgg
    17461 cttgtacacg tacttttttg tcgcgcccta aacgatataa ttcttcttgt gtttggatag
    17521 ctaattcacg tgttggtgcg ataactaaac cttgtaattc gtgacgatct ggatcgattt
    17581 tttctaacat tggtaatcca aatgcagctg ttttccctgt acctgtctgt gcttgtccaa
    17641 taacgtcttt tccagctaat gctagtggaa tcgtttctgc ttggataggc gttgcttctt
    17701 caaaaccagc gcgttcgact gatttcaaca attctggtga taattctagt tctttaaatt
    17761 tcaaatgaat cctcctatat ggttgttaac tgaggatgta gcttttttgc taccccgtcg
    17821 atttgttcgg tcaaagtgta ttgtttcttt ttgacagtgg ccacgcacgt ttttatccat
    17881 tcttagcccc tgtggcacca ccgaaaaatc actttgcgaa gtttaggcat tagaaaagcc
    17941 aaacgggtcg cgttcagctt tatcattatt atgacatctg gcttcaacaa gttgtttcta
    18001 tggcaaaacc agtccaattt ctcttcatct ttcggcaata ggttattcta acatagtttt
    18061 tcgttttctg caagtttaca gatttttatt tcatttgctg aagttgttct accacttcaa
    18121 ataagcccat gctgttgctg ccttttagca taatactatc tgttgcttca agattttgtt
    18181 tgatagcatc aatcaattta ttcttttctt tttcaaaata gaacacaggt agttgggggt
    18241 atttttggtt caattcttct tttagtgcat gcatttgttc cccataaagg aaaatcatgg
    18301 cgaatttctc aggatcgata tgttcactca tttgcgcatg catatttaaa gactctttcc
    18361 ctaattccag catatctgct aacacagcga ttctgcgtcc ttttgttggc agatttttaa
    18421 aagtatctaa taccagtccc atggctgttg gatttgcatt gtagacatcg ctcaaaatag
    18481 ctgcaccatt tcctgccgtt agccattcgg ttctgttttg agtgatagaa acatgggcga
    18541 gcccttggcg tatctcaggt atggacagtc caaagaagcg cccgatcgta tacgcaatca
    18601 aagcattcgt tacattataa cttccaggca gcgggatcgt aaatgtttct ccttctacta
    18661 caaaatcggt ttgtgctttt ccttttgctg tcacttccgc atgaacatcg ccatttccaa
    18721 taccgaatgt tgtgactgtt tgtactaatg ttgccgttaa aggtgtcagc aacggttcat
    18781 ctgcgggcac aacgagtaat ccgtctgata caagtccgtc agtgatttcc attttggctt
    18841 tagcgattcc ttctcgcgac ccaagatttt ctatgtgtgc ttcaccgatc attgtgattg
    18901 ctgcagcttt tggttgagcc ataagtgaaa gttccgtgat ttcattggcg tgatccatgc
    18961 ccatttccaa aacgatcttt tctgtatctt ggggcatgtg caaaatcgtg taaggaagac
    19021 caatattatt gttgtaattt ccttgcgtct tatatgtttt gtatttttca gacaagacag
    19081 acgcggttag atctttcgtg gtggttttac cattgcttcc tgtaattgca acgacttctg
    19141 cgcctacttt ttccaagtaa taggttgcca attgctgcat ggctttcaaa ggatctgcta
    19201 caggaataac tggaaaattt tttggagctt gttcaacagg caagctccaa aatgaagcag
    19261 atgctccgtt tgtgatcgct tgctggataa attcatgtcc atctcgcgta ccagcaagag
    19321 gaacgaataa acttccttcg gttatcttcc ggctgtcaaa ttcgacattg gtaatctttg
    19381 tcagtgcaga gcattcgctg cccacggctt gtgcaatttc ttgtattgtt agattcattg
    19441 agatctctcc taaaaaaggt tgtgaaaaaa gcgtttggcc taaacggcgg gttcgcatga
    19501 ctctcacttc caagtaaatt tctctattcg tatttagaag ctagaatgaa cgcggttacg
    19561 caatcactcc agcctcttct ttatttctct ttattcattt ttttcaaaaa aagattgacg
    19621 ctgatggtat cgattcattc ctaactggat cagttcttca atcaaatcac cgtatttcaa
    19681 gcccatattt tcccataaga gtgggtacat actgaactcc gtaaatcctg gcatagagtt
    19741 taattcattc aggaataatt catttttatt tgtcaaaaag aaatcgcacc ggctcaatcc
    19801 gcttccacct aacatcgtgt aagctaactt cgcgtactct tgcgcttttt gataaacttc
    19861 ttctggcact tcggctggaa tctgcatttc gattttatta ttgatatatt ttgcttcata
    19921 atcatagaat gctacgtctt ttacgacttc accaggcaaa gtcgtccgaa cgtcttcatt
    19981 tcctaataca gcaacttcga tttcgcgcgc ttcaattcct tgttcaacga ttgctcgaga
    20041 atcatactga taggctgttg ctaaagcatt ttgcagctct tctcggtttt ctgcttttgt
    20101 aatgccgaca ctagaaccca tattcgccgg tttgacaaac atcggataaa gcaaagaacc
    20161 ttcacattga tcaaatactt ttttaggatt ttctttccat tgattcttaa gtactggtac
    20221 ataaggaact tgcggcacac cagcagcttg taaaatatac ttggtcatga ttttatccat
    20281 ggcacatgca ctggtcaata cgcctgcgcc gacataaggc atattcaatg tctctaagaa
    20341 gccttggatc gttccatctt ccccgtttgg cccatgtaaa actggaaaaa cgatggctcc
    20401 ttcttctttg atttcgcccg gattgatcac ttttcctgta aagccttcct ctgtctgtcc
    20461 acttgggtcc catgaaagat gcaagacatc tttgctggca ggtttttctg ttagtaatgg
    20521 acctttgacc cattgtcctt cttttgtaat aaatacgagt tgaacttggt aataattata
    20581 ataaatggca tttaaaactg aaaatgcgga aagaatggac acttcatgct ctgcgctgcg
    20641 tccgccatat agtaaagtaa tcttcaaaag attgtcctcc tgtattctaa atcgttcagt
    20701 gatttactct catattttag cacaaaacaa aaataaacga ataacttatt tataaaaaaa
    20761 cgagccagct tctaaaagaa cgagcgtcat ttattttttg tcagctagat tatttgtccc
    20821 gttttttata aaaagttatg ataaagaaaa ggagggatga tgatggcgca aacaaatcca
    20881 cgcagccgct tggcagaaat cattcgagtg ttcatcaatt acaatgtagt gccaaacttt
    20941 gtacagcaaa aaaatcccga acaggtaaaa aaagcctttg aagaactagg ccccaccttc
    21001 atcaagatag gacagatgct ttctgttcga gaagatttgc tttcttctgc cttcactcaa
    21061 acattcaaaa cattacaaga tagtgtacct agcgacacat tttcaacggt caaaaagacg
    21121 atcgaaacag aactttctct ttcattatca gatatattcg atgatttttc aaaatcgcct
    21181 tttgcctctg cttctatggg gcaggctcat cgtgccaagc tgaagaacgg tgacgcggta
    21241 gtcgttaaga tccaacaccc taacatcgcc gaagagatcc gacttgatct tcaactattt
    21301 gaacgagcga tccctttgat caaatacatt cctgaaacta gcgtagttga tctaaaaggg
    21361 gtactccaag aagtaaaacg ttccttgatc aatgaaatgg acttcttaaa agaaagtcaa
    21421 aatggcgaac aattctatca aaagaacaat ggctggaaag agatccgcag ccccaagatt
    21481 tatgacgctt tttgttcgaa aaaagtgatc gtgatggaag aaatgtctgg taaaaacttg
    21541 aatcatttaa tgaacgccga aaacaaaact gaaactttta tcactggaat tcaaaacaag
    21601 cagctgaaac aagaagttgc aaagctttta gttgagaact ttatgaaaca ggtgtttgat
    21661 gatggttttt tccatgctga tcctcatcca gggaatctgc tattccatgt actaacaaaa
    21721 gaagaacaga cacaagctag ccgaaaaact gaaacagtcc atgaaaaaga gttcggttcc
    21781 tttgcttttc gagcctccac ttctgcagaa gaccctgttg cgccatatac catcaactac
    21841 atcgattttg gtatgatggg gcatctttct gcaggactta ggcaaaaact cacacaagcc
    21901 gttttagcgc tgtatacgaa agatgcttat cgaatcgaaa aagcggtcct tcgactttgt
    21961 cagcaagaag gctcgtttga tgaaagtcgg ttccaccaag aactgacaag ttttcttgaa
    22021 cagtattatg attctcctat tgatgaaatc aacttgcaag aagtattcac acatgtcgtg
    22081 acgatttgtc atcaaaataa tctgcagttc gatcgtgata tcacgctttt gctgaaagca
    22141 tttgggacat tagaaggggt gatccgcgtc ctcgatcctg aagtttccct catggaagtc
    22201 gcttcgcctt ttgctcaaca ttattttctc actcatctgg atgtagaaga tacattgaaa
    22261 caaagcggat tagacttatt ggaagggatg aaggctgcgc ctaagatccc tcaacagctc
    22321 caccatctat tagaaatgtg gacttcggga caaggaaaag tcaatttgga attgaaaaag
    22381 caagacaaat tattgagcag gatcgaaagt atgatcaatc ggcttgtttt cggtatgatt
    22441 ttagctgcat tgatcgtagg gtcctccctg cttgtccaag cagcacctgt agaaaacgca
    22501 gaggtcgttt ccttattagg gatatttacg tatgctattg ccgcattcgt cattattttt
    22561 ttggcaatcg atgcattgat ccaaatgtat aagaagagga aaaagtagaa aaaggttgtg
    22621 acagaaagaa tcagcttcaa gaaataagaa ggggttttac ataaattgtt tctcaaattt
    22681 ttgtgaaaat tcggcttatt tttcgaagag gctacttctg aaacaccgag aataaaaaat
    22741 gtgaaaaagc tgttctgcac ccagtaataa gaacaatcaa aaagggaaca tggaacacat
    22801 ttatcctgtt tccctgttcc ctttttgatg tattcgagca taaataaaat accttcgtta
    22861 cctactatct tctgtacttg gtaaacctta gtcaattgcc ttttttaacg cttgacgaaa
    22921 agacatatga tctttctccg taaagggttt tggacccttt gtccttatgc cagcttttct
    22981 taactttcca ctatgataac gttgttctag aagcgtgccg atattcgctt cgtcaaattc
    23041 ttttccagag tgatggaaca ctcgcacacc cttcggcaat acaagtgaag ctaaaccata
    23101 atcttgcgtg atcaaaaagt cattcttctt aaccataccg acgattttat aatccgcact
    23161 atcagctccg cggtctacgt agacgatttg gacatgatta gcgtattctt tggtcgtata
    23221 atggtccaca ctggtcacga taaccacgtc aagaccatag gattctccta attttatgac
    23281 atcttctttt actggagaac catctccatc aatgaatagc ctcattcttt ttcgccttct
    23341 tccataaatg ataaacagtt actaatccgc tcagtaaaag accaacattg atccattttg
    23401 ccaagataag cagttgtaca ctttctatcg gcactgattc atggttcagc gtgatcaata
    23461 atagatggat tggctctccc atttcttttg ctaaaacaaa caaaagcgtc atcaataaaa
    23521 actggtggat cacactccct ttattttgag tagtatactt gacaacgatc cggcgatcca
    23581 ttggaatagc tgtatcagtt tgataatatt ccttatttag ccagctgcct gttactacgt
    23641 gccagaaact aagcagaaag acacctatcg caaaaccatt tggaatcaag aggaccaata
    23701 aagaagaact cagtccaagc aattgcatag ccaatggaga ccatgttaaa cgtttcaaaa
    23761 tccatggtcc gactattttt gctgcaatca agcctgctgc ataaggcaaa tacataaaaa
    23821 tcgaaagttt ttctaatccg tacagtatgc caacataaaa actgctaaat aaaaaaagga
    23881 aattccctag cattccattt agaaaagtaa acagatttag ccagcttggc aacttccatt
    23941 ccttcttctg gtgtcctaaa acaaacacga acaagatgaa cagcagcaag aaaccataaa
    24001 tagcaagatc aaaaagcctt tcatcgaata acaatcgggc atttctcaaa agaaacaata
    24061 agccaaagaa tatcaaaaat aaaacaagct ctcgcgtagc taccagattt cttttgatgt
    24121 ctttgaaatc cagttcgtaa cgcggataat gactaaccgt atggtaggcc atgacgtaaa
    24181 aaagtgtata aatagtaaag cttgctgcaa tctgagtcgg tgcaggcaac tcaatcgctt
    24241 gatacaggag aaatagccag atagctgcaa ataagtactt tttcccatcc atattgataa
    24301 aaccttgcgt cttttcgtga aaattgaccg tggtattagc aggtacaagc catgctgcac
    24361 tcagccctag caaaacactt gaaaaataaa ataaaggaaa ataaaatacg cctaatattc
    24421 caagcaatga accagcacca ccaatcagca gagcgatcat cagcaacgtg taactatcta
    24481 gaccaaatcg tagactgttg actaaaaata gtccagtcat ccgaaaggca taaaacagcg
    24541 taaatggcaa aatcgtcagc caattatgcg agtctgttag tcccaaaaat aaaagataag
    24601 gaataaaaat aaccgtgtta cttaagataa agggcgttgt ttgtgtaaaa ttacgcccaa
    24661 atcgtttgag tttttcgaac atgacaatgg ctccttaaac agtcttataa ctaatctgct
    24721 cttcgatttt agcattaaag gggaaataaa ccaattttac agaatatatt cttgtaaaaa
    24781 aaaatacaga aagaagcata agcttctttc tgcattttta cggtacttga ttactcaagc
    24841 agacaagccc ttctccacaa aagtcttgcc cgacacccct gcatccggca ggtgataaag
    24901 atagaaatca attatcctgt gcctccaaaa tcaattagga gctctgtaaa ccagagtcct
    24961 tctcgacccg tcgcataaat ttactttaac acagttccta tgtttatgcg agagcttttt
    25021 tcattttata atcgaccatt tgaaatcctt gaccgatatc tgaaataagt tcttctgtaa
    25081 tctcaaatcc ttcatgctta taaacctcga ttgcttgatg attcccctga ttcactcgca
    25141 aaaagatctc tctttccttt gtttgcgcag caagttcttc ataccagcga aaaactgaag
    25201 aagtcaatcc ttgaccacgt actttattta gcaaatataa tttacttaaa taaatttttc
    25261 catttatctc ttcataagca gtgtaaccga cagtcttttc ttcgcttttt aataaaaagt
    25321 aggacacacc ttctgtcagt tctttttgaa tctgtttttt tgactgataa gttttcagca
    25381 tgtaatccac ttgtttttga ccaatgatcg gcacaaaaac ttccggccat acttcttgta
    25441 tgactgccat cagctcatcc agttcatttt ctgttacttt tattttttcc atacacccac
    25501 gtcctttttt acattataaa tgaccttgcg cttgatggaa taaaaagagc atgatcatgc
    25561 tatacacacc caccgaccac ggtttcaaga aaatcacaat ctgcataaat tccctaaaag
    25621 ataatttcgt caaaccagct actagacaga tcaagtcgtc tggcgcaaag ggaagcagca
    25681 aggtaacaac tagtactttt ttaatatctt tcgtttttct tgtcagcaat tcatcaaatt
    25741 tctgatactt ttttggcgat aaaataagct ggacaaaggc tacgccatag taacggacaa
    25801 gcaaaaatcc gattgcttcc ccaatcacta ggccaataca actatataaa agcccaaatc
    25861 cattgccaaa catcagcatg cccgctactg aggagatccc tcccggcaaa ataggaatga
    25921 tcacttggat cacttgcaac agaacaaata tcatcactgc atacttgcca aattgctgga
    25981 taaacgcttg caaagacgtg ctcgatcgaa aaatcccatg atgataagca taaagaatca
    26041 acacaaagaa aaaaaacaga ccaataatgg gcatgatctg aagacatttt tgtaaaactg
    26101 atcctttttt agacatctat attccccaca tcttttttca tttattaaaa ctgcctcct
//
LOCUS       DAZULO010000029        25331 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000029 DAZULO010000000
VERSION     DAZULO010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.030"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..84
                     /locus_tag="ACG8OA_001217"
     tRNA            12..84
                     /locus_tag="ACG8OA_001217"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ACG8OA_001218"
     tRNA            88..160
                     /locus_tag="ACG8OA_001218"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ACG8OA_001219"
     tRNA            168..249
                     /locus_tag="ACG8OA_001219"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ACG8OA_001220"
     tRNA            267..339
                     /locus_tag="ACG8OA_001220"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ACG8OA_001221"
     tRNA            351..422
                     /locus_tag="ACG8OA_001221"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ACG8OA_001222"
     tRNA            439..524
                     /locus_tag="ACG8OA_001222"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ACG8OA_001223"
     tRNA            535..608
                     /locus_tag="ACG8OA_001223"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ACG8OA_001224"
     tRNA            618..691
                     /locus_tag="ACG8OA_001224"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ACG8OA_001225"
     tRNA            711..783
                     /locus_tag="ACG8OA_001225"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ACG8OA_001226"
     tRNA            811..884
                     /locus_tag="ACG8OA_001226"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ACG8OA_001227"
     tRNA            898..971
                     /locus_tag="ACG8OA_001227"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ACG8OA_001228"
     tRNA            995..1084
                     /locus_tag="ACG8OA_001228"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ACG8OA_001229"
     tRNA            1097..1170
                     /locus_tag="ACG8OA_001229"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ACG8OA_001230"
     tRNA            1189..1264
                     /locus_tag="ACG8OA_001230"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ACG8OA_001231"
     tRNA            1266..1336
                     /locus_tag="ACG8OA_001231"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ACG8OA_001232"
     tRNA            1350..1423
                     /locus_tag="ACG8OA_001232"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ACG8OA_001233"
     tRNA            1448..1535
                     /locus_tag="ACG8OA_001233"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ACG8OA_001234"
     tRNA            1557..1628
                     /locus_tag="ACG8OA_001234"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ACG8OA_001235"
     tRNA            1651..1740
                     /locus_tag="ACG8OA_001235"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ACG8OA_001236"
     tRNA            1753..1826
                     /locus_tag="ACG8OA_001236"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ACG8OA_001237"
     tRNA            1845..1917
                     /locus_tag="ACG8OA_001237"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ACG8OA_001238"
     tRNA            1921..1993
                     /locus_tag="ACG8OA_001238"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ACG8OA_001239"
     tRNA            1998..2068
                     /locus_tag="ACG8OA_001239"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ACG8OA_001240"
     tRNA            2081..2154
                     /locus_tag="ACG8OA_001240"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ACG8OA_001241"
     tRNA            2166..2237
                     /locus_tag="ACG8OA_001241"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /gene="metK"
                     /locus_tag="ACG8OA_001242"
     CDS             2503..3696
                     /gene="metK"
                     /locus_tag="ACG8OA_001242"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFS4976040.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ACG8OA_001243"
     CDS             3826..5298
                     /locus_tag="ACG8OA_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS4976041.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ACG8OA_001244"
     CDS             5381..6169
                     /locus_tag="ACG8OA_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4976042.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ACG8OA_001245"
     CDS             complement(6236..6904)
                     /locus_tag="ACG8OA_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFS4976043.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ACG8OA_001246"
     CDS             7019..8731
                     /locus_tag="ACG8OA_001246"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4976044.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10503
                     /locus_tag="ACG8OA_001247"
     CDS             8734..10503
                     /locus_tag="ACG8OA_001247"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4976045.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10708..11274)
                     /locus_tag="ACG8OA_001248"
     CDS             complement(10708..11274)
                     /locus_tag="ACG8OA_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4976046.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(11274..12230)
                     /locus_tag="ACG8OA_001249"
     CDS             complement(11274..12230)
                     /locus_tag="ACG8OA_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFS4976047.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(12230..12886)
                     /locus_tag="ACG8OA_001250"
     CDS             complement(12230..12886)
                     /locus_tag="ACG8OA_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFS4976048.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            13256..15274
                     /locus_tag="ACG8OA_001251"
     CDS             13256..15274
                     /locus_tag="ACG8OA_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4976049.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    15344..15550
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15701..18115
                     /gene="leuS"
                     /locus_tag="ACG8OA_001252"
     CDS             15701..18115
                     /gene="leuS"
                     /locus_tag="ACG8OA_001252"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFS4976050.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            18463..20016
                     /locus_tag="ACG8OA_001253"
     CDS             18463..20016
                     /locus_tag="ACG8OA_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFS4976051.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(20397..22181)
                     /locus_tag="ACG8OA_001254"
     CDS             complement(20397..22181)
                     /locus_tag="ACG8OA_001254"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4976052.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(22196..23917)
                     /locus_tag="ACG8OA_001255"
     CDS             complement(22196..23917)
                     /locus_tag="ACG8OA_001255"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4976053.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            24017..24778
                     /locus_tag="ACG8OA_001256"
     CDS             24017..24778
                     /locus_tag="ACG8OA_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4976054.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7841 a         4653 c         5378 g         7459 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
    10561 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
    10621 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
    10681 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
    10741 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
    10801 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
    10861 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
    10921 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
    10981 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
    11041 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
    11101 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
    11161 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
    11221 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
    11281 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
    11341 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
    11401 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
    11461 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
    11521 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
    11581 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
    11641 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
    11701 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
    11761 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
    11821 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
    11881 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
    11941 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
    12001 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
    12061 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
    12121 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
    12181 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
    12241 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
    12301 acagccaaga aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca
    12361 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
    12421 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
    12481 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
    12541 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
    12601 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
    12661 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
    12721 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
    12781 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
    12841 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
    12901 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
    12961 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
    13021 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
    13081 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
    13141 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
    13201 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
    13261 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
    13321 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
    13381 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
    13441 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
    13501 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
    13561 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
    13621 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
    13681 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
    13741 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
    13801 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
    13861 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
    13921 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
    13981 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
    14041 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
    14101 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
    14161 ggaaaatggc tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg
    14221 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
    14281 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
    14341 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
    14401 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
    14461 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
    14521 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
    14581 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
    14641 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
    14701 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
    14761 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
    14821 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
    14881 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
    14941 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
    15001 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
    15061 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
    15121 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
    15181 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
    15241 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
    15301 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
    15361 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
    15421 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
    15481 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
    15541 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
    15601 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
    15661 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
    15721 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
    15781 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
    15841 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
    15901 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
    15961 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
    16021 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
    16081 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
    16141 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
    16201 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
    16261 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
    16321 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
    16381 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
    16441 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
    16501 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
    16561 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
    16621 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
    16681 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
    16741 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
    16801 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
    16861 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
    16921 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
    16981 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
    17041 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
    17101 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
    17161 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
    17221 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
    17281 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
    17341 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
    17401 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
    17461 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
    17521 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
    17581 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
    17641 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
    17701 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
    17761 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
    17821 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
    17881 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
    17941 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
    18001 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
    18061 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
    18121 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
    18181 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
    18241 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
    18301 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
    18361 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
    18421 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
    18481 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
    18541 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
    18601 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
    18661 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
    18721 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
    18781 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
    18841 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
    18901 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
    18961 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
    19021 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
    19081 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
    19141 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
    19201 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
    19261 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
    19321 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
    19381 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
    19441 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
    19501 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
    19561 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
    19621 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
    19681 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
    19741 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
    19801 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
    19861 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
    19921 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
    19981 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    20041 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    20101 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    20161 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    20221 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    20281 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    20341 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    20401 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    20461 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    20521 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    20581 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    20641 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    20701 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    20761 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    20821 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    20881 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    20941 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    21001 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    21061 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    21121 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    21181 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    21241 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    21301 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    21361 aaggcgagtt gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca
    21421 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    21481 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    21541 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    21601 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    21661 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    21721 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    21781 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    21841 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    21901 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    21961 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    22021 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    22081 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    22141 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    22201 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    22261 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    22321 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    22381 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    22441 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    22501 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    22561 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    22621 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    22681 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    22741 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    22801 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    22861 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    22921 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    22981 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    23041 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    23101 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    23161 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    23221 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    23281 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    23341 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    23401 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    23461 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    23521 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    23581 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    23641 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    23701 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    23761 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    23821 gtacacctaa tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc
    23881 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    23941 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    24001 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    24061 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    24121 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    24181 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    24241 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    24301 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    24361 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    24421 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    24481 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    24541 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    24601 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    24661 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    24721 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    24781 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    24841 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    24901 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    24961 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    25021 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    25081 gagaatgata aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac
    25141 attattacaa tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt
    25201 aatcaaatag tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt
    25261 tttgcttata aaataattat aagtatttag agatttttct tctaacattc ttcaaacaag
    25321 aaaaatcaca a
//
LOCUS       DAZULO010000030        24976 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000030 DAZULO010000000
VERSION     DAZULO010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.031"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            199..2733
                     /gene="secA"
                     /locus_tag="ACG8OA_001257"
     CDS             199..2733
                     /gene="secA"
                     /locus_tag="ACG8OA_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFS4976055.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2859..3960
                     /gene="prfB"
                     /locus_tag="ACG8OA_001258"
     CDS             join(2859..2930,2932..3960)
                     /gene="prfB"
                     /locus_tag="ACG8OA_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFS4976056.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4065..4751
                     /gene="ftsE"
                     /locus_tag="ACG8OA_001259"
     CDS             4065..4751
                     /gene="ftsE"
                     /locus_tag="ACG8OA_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFS4976057.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4744..5628
                     /gene="ftsX"
                     /locus_tag="ACG8OA_001260"
     CDS             4744..5628
                     /gene="ftsX"
                     /locus_tag="ACG8OA_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFS4976058.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            5780..7228
                     /gene="cls"
                     /locus_tag="ACG8OA_001261"
     CDS             5780..7228
                     /gene="cls"
                     /locus_tag="ACG8OA_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS4976059.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7565..8422
                     /locus_tag="ACG8OA_001262"
     CDS             7565..8422
                     /locus_tag="ACG8OA_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFS4976060.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8537..9457
                     /gene="pstC"
                     /locus_tag="ACG8OA_001263"
     CDS             8537..9457
                     /gene="pstC"
                     /locus_tag="ACG8OA_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFS4976061.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9457..10341
                     /gene="pstA"
                     /locus_tag="ACG8OA_001264"
     CDS             9457..10341
                     /gene="pstA"
                     /locus_tag="ACG8OA_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFS4976062.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10356..11162
                     /gene="pstB"
                     /locus_tag="ACG8OA_001265"
     CDS             10356..11162
                     /gene="pstB"
                     /locus_tag="ACG8OA_001265"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS4976063.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11178..11936
                     /gene="pstB"
                     /locus_tag="ACG8OA_001266"
     CDS             11178..11936
                     /gene="pstB"
                     /locus_tag="ACG8OA_001266"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS4976064.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11949..12626
                     /gene="phoU"
                     /locus_tag="ACG8OA_001267"
     CDS             11949..12626
                     /gene="phoU"
                     /locus_tag="ACG8OA_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFS4976065.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            13203..13895
                     /locus_tag="ACG8OA_001268"
     CDS             13203..13895
                     /locus_tag="ACG8OA_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS4976066.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            13892..14659
                     /locus_tag="ACG8OA_001269"
     CDS             13892..14659
                     /locus_tag="ACG8OA_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS4976067.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14759..16327
                     /gene="liaX"
                     /locus_tag="ACG8OA_001270"
     CDS             14759..16327
                     /gene="liaX"
                     /locus_tag="ACG8OA_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFS4976068.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16624..16944
                     /locus_tag="ACG8OA_001271"
     CDS             16624..16944
                     /locus_tag="ACG8OA_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFS4976069.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            16944..17294
                     /locus_tag="ACG8OA_001272"
     CDS             16944..17294
                     /locus_tag="ACG8OA_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFS4976070.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17461..18399
                     /gene="hprK"
                     /locus_tag="ACG8OA_001273"
     CDS             17461..18399
                     /gene="hprK"
                     /locus_tag="ACG8OA_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFS4976071.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18414..19244
                     /gene="lgt"
                     /locus_tag="ACG8OA_001274"
     CDS             18414..19244
                     /gene="lgt"
                     /locus_tag="ACG8OA_001274"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFS4976072.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19288..20313
                     /locus_tag="ACG8OA_001275"
     CDS             19288..20313
                     /locus_tag="ACG8OA_001275"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS4976073.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20328..21266
                     /gene="galU"
                     /locus_tag="ACG8OA_001276"
     CDS             20328..21266
                     /gene="galU"
                     /locus_tag="ACG8OA_001276"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS4976074.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            21391..21831
                     /locus_tag="ACG8OA_001277"
     CDS             21391..21831
                     /locus_tag="ACG8OA_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFS4976075.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21834..22436
                     /locus_tag="ACG8OA_001278"
     CDS             21834..22436
                     /locus_tag="ACG8OA_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS4976076.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(22495..23601)
                     /locus_tag="ACG8OA_001279"
     CDS             complement(22495..23601)
                     /locus_tag="ACG8OA_001279"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFS4976077.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            23811..24812
                     /gene="ccpA"
                     /locus_tag="ACG8OA_001280"
     CDS             23811..24812
                     /gene="ccpA"
                     /locus_tag="ACG8OA_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFS4976078.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
BASE COUNT         8224 a         4340 c         5425 g         6987 t
ORIGIN      
        1 aaaacttgca gcagctactg acagctgccg caagtttttt tgagtttcct cctcttttag
       61 atgaaatatg aataaattat gaagttctta ctttaatacg cttaagatat ttcaattatc
      121 tataactaat gataaaatga aaagtaaagt ctacatagtc tggataatac acagaaggaa
      181 aaggagatta cacaacgcat ggccaatttg attcgttcac ttatcgaaaa cgataagaaa
      241 gaactaaaac gtttagatag cattgcaaaa aaagttgaag catacgctga ccaaatggca
      301 gcattaactg atgaacaatt acaggcaaaa actgctgaat tcaagggcag ataccaaaaa
      361 ggagaaacat tagatcaatt acttccggaa gcttttgcag tcgtccgaga agcagcaaaa
      421 cgtgtattgg ggttatatcc acaccacgta caattgatgg gtggaatcgt gcttcacgat
      481 ggaaatatcc ctgaaatgcg tactggtgaa ggtaaaacct tgaccgcaac gatgccagta
      541 tatctaaatg ctctgactgg cgaaggggta cacgtagtta ccgtcaatga atatttagca
      601 acccgtgact cgacggaaat gggcgaactt tataatttct taggattaac agtggggttg
      661 aatatcaact caaaatcttc ggatgaaaaa cgcgaggctt ataattgtga tatcacttat
      721 agtacgaata atgaattagg atttgactac ttgagagaca atatggtcgt ttaccgccat
      781 caaatggtac aacgtccgct gaattatgcc attgtcgatg aagttgactc aatcttgatt
      841 gatgaagcga gaacgccgct gatcatttca gggcaagcag aaaaatccac agctttatat
      901 acaagagcag acaacttcgt gaaacgtttg aaagaagaag aagattataa aatcgatgta
      961 caatcaaaaa caatcagttt aaccgaagca gggatcgaaa aagcagaaga aacattcggt
     1021 ttggataact tatacgacat tgaaaatact gcattgaccc atcacttgga tcaagcattg
     1081 agagccaact atatcatgct tttagatatc gattatgttg tccaagacgg aaaagtactg
     1141 attgttgacc agttcactgg tcggatcatg gatggacgcc gttattcaga tggacttcac
     1201 caagcaatcg aagctaaaga aggcgtagag atcgaagacg aaacgaaaac catggcgacg
     1261 atcactttcc agaactattt ccgtatgtac aagaaacttg cgggaatgac tggtacagct
     1321 aaaacagaag aagaagaatt cagagaaatt tataacatcc aagtatacca gattccgaca
     1381 aatcgtccgg ttatccgtga tgatcgtcca gatttgcttt acccaacatt acaaagcaag
     1441 ttccacgctg tcgtgcaaga tatcaaagat cgctaccgca aaggtcaacc tgtgttagtc
     1501 ggaactgttg cggttgaaac atctgagtta ttatcagata tgctgaatca agaacgtatt
     1561 cctcatgaag tgctgaatgc gaaaaatcac tttaaagaag cagagatcat catgaatgct
     1621 ggtcaaaaag gtgctgtgac gattgctacc aacatggctg gtcggggaac agatatcaag
     1681 ttaggacttg gggtaagaga acttggtggt ctagcagtta tcgggacaga acgacatgaa
     1741 tctcgtcgta tcgataacca gcttcgagga cgtgcaggac gtcaaggaga ccctggtatg
     1801 tcacagttct acttgtcatt ggaagatgat ttgatgaaac gattcggttc agaacgaatc
     1861 aaatctttct tagatcggat gaagatcggt gaggaagatg ctgttatcca aagcaagatg
     1921 ctgacgaaac aagtagaatc tgcacaaaaa cgtgtagaag gaaataacta tgacacacgt
     1981 aaaaacgtct tacaatacga tgatgtgatg cgtgaacaac gtgaagtaat ctataaacag
     2041 cgacaagaag taatcatgga agaaaaaagc ttatccaagc cactaatgaa catggtcaaa
     2101 cggacaatca gtcgtgtagt agatgcgcat acacaattag agaaagaaaa ttggaatttg
     2161 gaaagtatca tcgattttgc cggcagtact cttgtccatg aagatagtat tactcttgca
     2221 gatatcgaag gaaaaacagc tcaggagatc aaagagtatc taaacgaacg agcacaagaa
     2281 gtatttaata ccaaagcggc tcaactaaat ggtccagagc aactgttgga atttgaaaaa
     2341 gtcgtgatct tgcgtgttgt ggactctaaa tggacagacc atattgatgc aatggatcaa
     2401 ttacgtcagt caatcggctt acgtgcttat ggacagaaca atccattagt cgaatatcaa
     2461 acagaaggct ataagatgtt tgaagatatg gtaggcgcca tcgagtatga agtcactcga
     2521 atctttatga aagctgagat ccgtcaaaac gtacagcgtg aacaggtttc tcaaggaaca
     2581 gcttctcact cagaagacgg agatgccaaa gaaaatagta atactagcgc gaaaaaacag
     2641 ccaatccatg tgaacaaggt cggaagaaat gatccttgtc cttgtggaag cgggaaaaaa
     2701 tataagaact gtcatggaaa aaatgcaaac taatttctga cagcaggatt ggtgagacaa
     2761 attctttttt taagaattcg tctcaccttc tttctgttat ttgctgattc tgttagacta
     2821 gatataatga aaaaagtata gtgaaagaag gaaaaaacat ggaaatatcc gaaattcgaa
     2881 atctcctgac agaaatgcag gaaaaggtca caagcttcag gaggtctctt tgacttagac
     2941 cagctggaag aagacattgc ggaagcagaa gcaaaaatgg ctgaacctgg tttttgggat
     3001 gatagcgaag ctgctcaaaa agtgatcagt gaaaacaatg caaataaaga aacttatgat
     3061 caattccatt ctcttgctga agaactggaa gaaatcgaat tgatggtgga gatgttgcag
     3121 gaagaacctg atgcagccat gcaagaagaa gcagaaacac gtatcaatgc gctagatgaa
     3181 aaaatgaaga catatgaatt atctatgttg ttagatggtc cttatgatcg aaacaacgcc
     3241 atcgttgaat tgcatccagg ggctggcgga acggagtccc aagattgggg cagtatgctt
     3301 ttacgtatgt atacacgctg ggcagagcag catgggtatc aagtagagac actagattat
     3361 caagcaggtg atgaagcagg aatcaagagt gtgaccctac tgatcaaagg acacaacgca
     3421 tttggttatc tgaaatcaga aaaaggtgtt catcgccttg tacgtatctc gccttttgac
     3481 tcagcgaaac gacgtcatac ttctttttgt tcggtagatg tgatgccaga gcttgatgaa
     3541 acgatcgata tagaaatcaa tccagatgat ttgaaaatcg atacgtaccg agcaagtggt
     3601 gcaggtggtc agcacatcaa taaaacagaa tctgctgtac ggatcactca cttgcctaca
     3661 ggaacagtag tagctagtca ggcacaacgt tcacaattga aaaaccgtga gcaagcgatg
     3721 gggatgttaa aagctaagct ttatcaatta gaagtggaaa agaaagaaca agaagcagct
     3781 gcacttcgag gagaacaaaa agaaatcggc tggggttcac agatccgttc ttatgttttc
     3841 catccatatt ctatggtaaa agaccatcgg acaaacgtag agacaggaaa tgtccaagca
     3901 gttatggacg gtgaccttga tttatttatt gacggctatt taaaaatgca tctacaataa
     3961 gaacccattg ttaaattgaa ataaaattgt aagattttgc aagcatgttg aaacattgcc
     4021 atgctataat agctaaagac tgaaaaagat atggagagaa cgccatgatt gaaatgaagg
     4081 atgtaatgaa gaagtactca aatggtacga ccgctatccg caatctctca attgtcatcg
     4141 aacaaggaga gtttgtctat gtggttggtc cgtctggagc cggtaaatct acattcatca
     4201 aattgatgta tcgagaagaa aaagcttcaa aaggaacttt gaacgtcgca ggtcatgatc
     4261 taatagcaat caaaaaccgc gaagttcctt atctacgcag agaaatcggc gtggtgttcc
     4321 aagactataa attattacca agaaaaacag tatatgaaaa tgttgcgtac gctatgcaag
     4381 ttatcggcag aaaacctcgg gatatcaaaa aacgagtaat ggaagtgctg gaccttgttg
     4441 gtttgaaaca caaagttcga gtctttccta gcgaattatc cggtggggaa cagcaacgtg
     4501 tctctattgc tcgggcaata gtgaatacgc ctaaagtact tatcgcggat gaaccgacag
     4561 gaaatctgga tccagaaaac tcatgggaaa tcatgaaatt attagatcgg atcaatgccc
     4621 aaggaactac tgtcgtgatg gctacacata acagtacgat cgtaaatacg attcggcacc
     4681 gagtaattgc tatcgaaaac ggccgcatta ttcgagatca agcggaaggg gaatatggat
     4741 acgatgatta gaaccttttt ccgccattta ttagaaagta tcaagagttt gaaacgtaac
     4801 ggctggatga cagttgcttc agtcagtgcg gtaacgatca cattgacttt agtcggtgta
     4861 ttcatggcag ttatcatgaa cgcaactaaa ttagctcaag atatagaagg aaacgttgat
     4921 gtctctgtat tcgtagatat cggtacaaaa gacgaagaga tgaaaaagct agaaaaagaa
     4981 ttgaatgact tagatcatgt taaaaaagtt gaattttcaa gcaaagaaca ggaattgaaa
     5041 aaaatccaaa acgaaatggg tgattcctgg aaattgtttg aaggtgacaa taacccgtta
     5101 tatgatgtgt atgtagtaag tgctactgat ccttcttata caaaacaagt agcagaagaa
     5161 gcagcagatt tgaagaatgt ttctcgttct gattacggtg gagcttcctc agatcggatt
     5221 ttccaaatcg ctggtgcagt tcggacatgg ggattagttg cagcggcatt gctgctgttc
     5281 gtagcgatgt ttctcatttc gaacacgatt cggatcacga ttttgtctag acagcgtgaa
     5341 attcaaatca tgcgtttagt cggtgcaaaa aatggatata ttcgctggcc gtttttcctt
     5401 gaaggtggct ggatcggtct gatcggtgcg attcttccga ttttgatcat cacatttggt
     5461 tatgcatggt ttttccaatt gatcaatcca tcattattac gttcccatta ttcattgatt
     5521 catccacaaa atatcgtttg gaaaatcaat cttttaatgg tcggcattgg tgtgatcatt
     5581 ggttcacttg gctcgatcat ttctatgaga cgtttcttga aattctaata ccacaatata
     5641 aataaaatga gaaagttgga cagtttcgtt ccagctttct ttttttattg gctaggagaa
     5701 tagaaggaag cggctttttt ccctttactt taaaaaacaa cttgggtatt cttaagttgg
     5761 tagaagaaga ggtgaagccg tgaaaattat cgttgacaat ttttttacga tccttttgat
     5821 tatgaatatt ttgctttcct ttatcatcgt ttttcgagaa agaaaggaaa ctgctcaaac
     5881 atgggcatgg gtgctcgttt tgatgttcat tccagttgta ggatttattt tatatatttt
     5941 tttaggaaga gggatttcca aagagaaaat ctttgatttg aagatccaag ggaaaatcgg
     6001 taagaacctg gaaattgaag aagacaaaca ggcattgatg cgagggcttt atcctcatcc
     6061 gcctactgga caagtagatg tgaaacaatt gatctatatg ctcacggtat ttgaaagtac
     6121 gctctatacg actaaaaatg aaatcacttt atttacagat ggtcgtgaaa aattcgatgc
     6181 gctgattgaa gatatcaagc aggcagaaga ccatatccac ttccagtatt atatttatcg
     6241 cagtgatgca ttaggtgaag aagttcgaga tgcattgaca gaggcggcaa gacgaggggt
     6301 aaaagtcaga gtccttcttg atgcatgggg ttccacacaa gtttcttcca gcttttttca
     6361 gaatttgaaa aaagcaggcg gagaagtcgc atttttcttt cctctatttg ttccgtatat
     6421 taatcctcgg atcaattatc gcaatcatcg taaaatcgta gttattgatg ggaaaatcgg
     6481 atatacaggt gggttcaatg ttggaaatga atatctagga ttggtaaaga aatttggtta
     6541 ttggcgagac aatcatttgc gtatttacgg agaagcggtg tataccttgc aaaaccgttt
     6601 tttgatggac tggaattctc agcatacaaa agaagtaggc tattctccca aattttttcc
     6661 gtccattcac tctactggtg atatagctgt tcaaatcgtg acaagcggac cagataccga
     6721 acatgaacaa atcaaaatga cttatttgaa aatgatttcg ttagccaaac gggagatatt
     6781 gatccaaacg ccttattaca taccggatgg atcgattcat gaagcactga agttggctct
     6841 tctttcaggc gtaaaagttc atatccaaat acctaacaaa ccagatcatc ttctcgttta
     6901 ctgggcaacg tattctttcg ctgcagagct aatcgagtat ggagcaagga tcgaaacata
     6961 tgagaatgga tttattcatg caaagacgat gattatcgat ggtggcattg tttcagttgg
     7021 ctcagcaaat atcgatgttc gttcatttcg cttggacttt gaggtcaata cactggttta
     7081 tgatgaacga atggcttcac gtgtaagaaa agcttttttt gaagactcga aaatttctac
     7141 gcatctcaca aaagaaatgt atgaaaaccg aggaataatc atcaagatga aagaaggatt
     7201 agcaagattg atttcgccgc tcctttaaaa cataaaaagg acatgagaac catcaacaga
     7261 tggagatctc gtgtcctttt ctaacggggc catttctgag agtacgtcag aaatggagaa
     7321 agcgtctcgc atgagcgaaa ttaactttgg ggtatctctt gttttttggg gaaagcaaga
     7381 aataagctaa tatatgctat taaaagagac acatttcaaa gccagtatag catagaaact
     7441 tttaatgaga tagttttttt tacaattatg agaatttatt ttgttattta atgattttga
     7501 ttcgttttca tcaagtaatg aaaatgataa aataaacata ctttaggatt aaaggagatt
     7561 tctaatgaaa aaacttaaat tattcttacc aataatagga ttagctattc ttttatcagg
     7621 ctgttcaaag tggattgatc gaggagaatc cattacggct gttggctctt ctgctttaca
     7681 gcctttagta gaaacagttg ctgaaactta ccagacagaa aatcctggaa aattcatcaa
     7741 tgtacaaggg ggcggaagcg gaactggttt gtctcaagta cagtcaggag cagtcgacat
     7801 cggaaactct gatttgtttg cggaagaaaa atcggggatc gatgcctcag cattagtcga
     7861 tcatcgagta gcagttgttg ggatcactcc tattgtgaac aaggatattg gcgtaaaaga
     7921 catcagtatg gaaaacctga aaaaactatt tacaggcgag atcaaaaact ggagtgaagt
     7981 cggtggaaaa gatcagccag tagttatatt gaaccgcgct tcaggaagcg gaacccgtag
     8041 cacgtttgaa aaatgggtat tggacggaga aacagctgtt caggcacaag aacaagattc
     8101 aagcggaatg gtgcgccaga tcgttgccga cacacccggt gcaatcagct atgttgcttt
     8161 ttcttatgtt tccgatgatg tagatacgtt gaatgttaac ggtgtagaac cgacagatga
     8221 aaatgtgaca acgaacaaat ggacgatttg ggcatatgag catatgtaca caagagatca
     8281 tccaaaaaaa ctgacaaaag aatttttaga ctatatctta tctgatgaag tacaagataa
     8341 catcgttgga gaactaggtt atatccctgt ttctcatatg aaagtcgaac gtgattggca
     8401 aggaaatatc gttaaagagt aatctttaca caaaatttac aaacttagct aaacttttac
     8461 atgttattaa aaccaaattg atgttcaatt tgtacactga gtgagtatgt taaacctgat
     8521 taagggggag tcatctgtgg aagatgtgaa aaaagtatta ttaacaaaat caaaacgttc
     8581 aaaaatggaa cagcgtggaa aattcatcag ttttttatgt atcgcgctga tcgtgttggt
     8641 tgtattatct attttttatt ttgtagctag taaaggattg gccacctttt ttactgataa
     8701 gatcaatgta tttgattttc tgtttggtac agattggaat ccaagccaaa tcggagcaaa
     8761 tgggaaacca atggtagggg cattgcctat gatcacaggt tcgtttattg tgaccttttt
     8821 atctgctatc gttgccacgc catttgccat tggtgcagca gtattcatga cggagatctc
     8881 tcctaaaaac ggaacgaaga tcttacagcc ggtcatcgag ctgcttgtag gtattccttc
     8941 tgtcgtttat ggttttatcg gtttatcagt tatcgtgcct tttgtcagaa gtatttttgg
     9001 cgggactggg tttggtatct tagctggtac atttgtgttg ttcgttatga tcttgccgac
     9061 tgtcacaacg atgactgttg atgcattaaa ggcagtgcca cggcactatc gggaagcttc
     9121 attagcgctt ggtgccacaa gatggcaaac aatctacaaa gtcgtgctac gagcagccgt
     9181 gcccgggatt ttaacagcag tcgtttttgg tatggctcgt gcgtttggtg aagccttagc
     9241 tatccaaatg gtcatcggta atgcagcatt gatgccaaca ggtcttacta ctcctgcttc
     9301 tacattgacg tccatcttaa caatgggaat cggaaataca atcatgggaa cagttgaaaa
     9361 taatgtgtta tggtcattag cgctgatctt gctattgatg tcattattct ttaatatcgt
     9421 gatccgtatg attgggaaga aaggagagct gaaataatga acgcaaaacg ctcagataaa
     9481 attgctacag gtgttttata tatagtttcg gggatcatcg tattgatttt agcagcttta
     9541 ctactttata tcttagttcg gggaataccg cacatttcat ggtccttctt gaccgaacct
     9601 tccaaagcct accaagaagg cggtggaatc gggatccagc tatttaactc gatttatttg
     9661 cttttgatca cgatgatcat cagttttcct atttctttag gtgcgggtat ctatctttca
     9721 gaatatgctg gaaaaaactg gctaacagat gtgattcgca cgtcgattga aatcttgagc
     9781 tcgttgcctt ctgtcgttgt cggtttattc ggtttcttgg ttttcgttat tcaattccag
     9841 tatggtttct cgattatttc tggtgcattg gctttgactg tattcaactt gcctcttttg
     9901 actagaacag ttgaggagtc tttacaagcg gtccattaca cacaacgaga agcaggattg
     9961 gccttaggtt tgtcacgctg ggaaacagtg ataaaagtgg tggttccaga agcgacacca
    10021 gggattttga ctggtgtgat cctaagttct ggaagaattt ttggtgaagc agctgcattg
    10081 atctatacgg ctggacaaag tgcacctgcg ttagatttta cgaactggaa tccattaagt
    10141 gtttcaagcc cgatcagcat tttccgtcaa gctgaaacat tagctgtcca tatttggaag
    10201 atcaatacag aaggcacgat gcctgatggt gtttctgttt cggctggcgc ttctgccgtg
    10261 ttgattattg ctgttttact gttcaacttc ggcgctcgtg cgattggcaa acgattatac
    10321 cgaaaaatga cttcagccta aggagaagaa gcaaaatgaa aaaatataac tggaatgaaa
    10381 caaatgtcat caccatgcca aaagagaaag aagtagcact atatacggat gatctacatg
    10441 tctggtatgg cgacaatgaa gcaatcaaag gagtggatct ggaatttgaa aaaaataaga
    10501 tcactgcact gattggtcct tccggctgcg gaaaatctac gtatcttcgt tcgttgaatc
    10561 gaatgaatga tgggatcgct aatacgaaaa tcactgggaa aatcatgtat caaggcgtag
    10621 atattaatgc gcctcaagtg gatgtttatg agatgcgcaa acggattggc atggtttttc
    10681 aacgtcctaa tccgttcagc aagtcgattt atgacaatat tacatttgct ttgaaacagc
    10741 acggcgaaaa aaacaaaaag aaattagatg aaatcgttga aacaagcttg aaacaagctg
    10801 ccttgtggga ccaagtaaaa gacagcttgg acaaaagtgc cttggcttta tctggcgggc
    10861 aacagcaacg tttatgtatt gctcgtgcga tcgcgatgaa acctgatatc ttgctgctag
    10921 acgagccggc aagtgctttg gaccctattt cgacaggaac ggttgaagaa acacttgtaa
    10981 acttgaaaga agactatacg atcatcatcg tgacgcataa catgcaacaa gcagctcgta
    11041 tcagcgacta tactgccttt ttctatttag gaaaagtaat ggaatatgac aaaactaaca
    11101 agatatttac cagaccaaaa attcaggcaa ccgaagatta tgtgtccggt cattttggtt
    11161 aagacggagg cctcaacatg acaaaagaga ttatttcatc taaagatctt catctgtatt
    11221 atggaaagaa agaagcacta aaagggatcg atctaagctt caatcaaggg gaaatcactg
    11281 cgatgatcgg tccttctggt tgcggaaaat ccacttatct gcgttctttg aaccggatga
    11341 atgacctgat tccgaatgta acgatcaccg gaagtgtttt atataaagac aaagacatct
    11401 atggtccaaa aacagatgtt gttgaactaa gaaaagaaat cggcatggta ttccagcagc
    11461 caaacccatt tcctttttct atctatgaaa atgtcatcta tggattgaaa ttaaaagggg
    11521 aaaaagataa aaaagtcctt gatcaagtag ttgaagaaag cctgaaggca gcgtctgttt
    11581 gggatgatgt gaaagacaaa ttgcataaaa gtgctttatc tttatccggt gggcagcagc
    11641 agcgtgtatg tatcgcacgt gttttggcag ttaatccgga aatcatctta ttggatgaac
    11701 caacaagtgc gcttgatcct gtttctactg ggaaaatcga aagtatgctg ttagaattaa
    11761 gagagcggta tacgatgatc atcgtgaccc ataatatgtc tcaagcttca cgtatttcag
    11821 ataagacggc attcttttta gatggtcact tgatcgaata caatgatacg aagaagatat
    11881 tcatgaatcc agaaaaacaa gagacagaag attatatttc aggtcgattt ggataaggag
    11941 gaaacggaat gctacgtaca caatttgaag aagaattgct gaatctccat aatcagttct
    12001 acgaaatggg gatgatggtg agcagcgccg tgcacaagtc agttcgtgca tacatcaaac
    12061 acgataaaat cttagcccaa gaagttatcg aaaacgatat aaatatcaat aacatggaaa
    12121 cacgcttgga gaagaaaagt tttgaaatga tcgcattgca acagccagtt acaactgatt
    12181 tgcgtatgat cattacggtc atgaaagcta gctcggattt agaacgtatg gccgatcatg
    12241 cggtgtctgt tgctaaatcc acgattcgcg taaaagggca gacgagaata ccggaaatcg
    12301 aaaaagagat ctccgatatg tccgattatg tgaaaaaaat ggtggacaac gtcctagttg
    12361 cttatgtcaa aacagacgaa aaagacgcac gtacgatcgc caatatggat caaagagtca
    12421 atgactattt caatcgaatc tataatgcaa caatcaaaaa tatgcaggaa aatcctgaga
    12481 cagtcatcag tggaacagac tacttgaatg ttgccagtta tctagaacgg atcggagact
    12541 atgtgacaaa catctgtgaa tggatcgtct acttggctac agggaaaatc actgagttaa
    12601 atacgaacca caatgagatt ttctaaactg aagctgtgat tatgccgttt gtttaggaaa
    12661 agaggttgtg aaatcagcgg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagtc
    12721 aaacaaaaaa tagctcctca tatttttcgt ttggctggac ttattaccgc agatgcaagc
    12781 ttttgaacac cgtttattcg ggaaagggtt tgtgacaaaa catgtcacaa acccttttca
    12841 tcatttcata gaaaggataa agaatgcaaa aaaaaattag catcatcact atatttttta
    12901 tttgtgtgct tacttcatgg gttattcagc atcaagccgc tttatatgaa gtacctgtca
    12961 tcgagataga aaaagtacga ataaaagagg atcatcaatt gattacagga aaactgatga
    13021 acacggatca aaaaggagaa tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag
    13081 aaacagcaaa tataaaagcg agacaaatgt atctagtcca aatgcatgga gatgaatggc
    13141 agctagtagc aaaaaaaaat gatggctggc ttttcttctt cacaagtatc ttcattggcg
    13201 ccatgttgct tattggtgga aaacagggtc tatttgcttt aggaagtctc atcatcaata
    13261 tcgttttact tttacttttg ttgctcatct ttactaaaac agaagggatt tctctattaa
    13321 atatatctat cctgttcgtc gttataggga tgattattag catattggct ttagatggat
    13381 ggaatagaag cagtataatc aaaatttcag ctgcagttac gagtgttttt ttagcatttt
    13441 ttatttgtct tttgacaatg aatcattgga aagaccaagg actgcgctat gaggaactga
    13501 attatctgac acgaccgtat cgttctgttt ttctttctag cgtactgatt ggaacagctg
    13561 gaggaacact cgataccgtt atcacagtca ttgcgacatt agaagaattg actaagcgac
    13621 aaccagaaat aacagcgaaa cagttatggc tttcaggaag gcaagtgggt cgagatgtga
    13681 ttggctcgat gaccaatatc cttttgttcg tctatatcag tgggtcagtt ccatcgcttc
    13741 ttttatattt aggaaatcaa tggtcgttca aagaaacgat agaacagcat ttatcattgg
    13801 aatttttacg cgtcattgct ggcagcttag gaatagtatt atcgatccct atctcggtcc
    13861 tcttttttct gactgtaagg aggctgaaac gatgaatgta cttattatac ttgttgggat
    13921 tttagcaatc agcgtgctac ttattggcgg tacgcaaggt atgtatattc ttttaggctt
    13981 attcatcaat ttagggatct ttttcctgtt gttattcggt tatcatcaga agtggccgat
    14041 actggcattg tcggtcatag gatttttatt gattgctgtg gttatcttat tttttatcaa
    14101 cggttataat ctaaaaatgc gtgcagcttt tgtgagtata ctgatctttt tattttgttt
    14161 cctactattg cttcctatca ctgatttttt agcaatccaa ggttttacat cggtagaact
    14221 agaagaatta tctggcttag ataagacttt agcgattgat tttagattac ttacgagatc
    14281 gttgcttctg attagtctta gtggtgctgt tttagatgcg agtgtagcaa tttctagtgg
    14341 gacattcgaa gtatatcagg caaatccgca attatctttt taccaattgc gccattctag
    14401 tttcgctatt gctaaaaaaa tattggcgtc aacagtaatg acacttcttt ttgcattcat
    14461 gggaagttct ttagcgttga tcctctggtt tattgattta gacattcctt ttcagcaagt
    14521 gatcaatgaa aaatcatttg ttctagaata tacgatggct attcttacca cattgtccgc
    14581 actacttgtt cttcctttaa cctgcgtgac tacagcatat ctctttacaa aaaagaaaga
    14641 acaactcaag atggagtaaa agttacaact taagtcctat gacattttta ctgaatcagt
    14701 gcataatatg gatgtaagga aaagcttgca aacaattttg cttaaggagg caatttccat
    14761 gaaagaaaga gaacgtattt tagatttagt gaaaaaagga atcctttcga ctgaagaagg
    14821 cttagattta ttagaaagca tggcaactga aaaagacgaa aagcaaatca aaaaagaagc
    14881 agacaaggta actgctagtc ataaagaaaa agatcaggca agtcaattga tcgataaact
    14941 tgaaaacggc gaagaagaaa tttctgagcc agttgatcca aaggaaagag aaagacaaga
    15001 tcaagaaaac ttagaaaaga tcttagatga actagcaact gaagcgaata aaacttcggc
    15061 gcgtctagat gaagtaaatg ctcaattcgc tgacaataag gcagcacgca atgagaaaca
    15121 ggaagcattg atgcagctga atacaaaaga agaattaggc gagttgacag aagaagaatt
    15181 agcacagcgt cagacgttag aagcagaaat caaagaatta gaagctagtg gcgatacact
    15241 gttagaagaa caattgaaat tagaagcaga attaaaagat atccgtaaga atcaatggtc
    15301 tgagaaaaaa gaaacgttta ctgataagtt tgaattgcca gatgattgga aagatcaagc
    15361 aacagataca ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac agttaggcaa
    15421 attcttgaag aaaacattcc aaactgtttc agaaacagtg aatgacaaca tggaatggaa
    15481 agatgtcagc ttacgtgtcc ctgggattgc gacaacgaaa tttgagcatg aattttacta
    15541 tgaagctcct gcagcaagta tccttgatat caaggcagca aacggaaacg tgacgttaaa
    15601 aacgtgggac agcaacgatg tgaaagttga agctaagatc aaactttacg gaaaaatggg
    15661 tgctgaacca tttgaagcat tctctgaacg gagccagatt gaggttaatg aagatcatat
    15721 ctccttccaa attccaaata aacgtgtgcg tgcagacctt gttttctact tacctaaacg
    15781 agtatacgac cacgcagcta ttaaacttct aaatggcaac atcatgattg aaacattgga
    15841 agctaaagat atttatacaa aatctacgaa tggtaacatc atcgtggacc aattaactgc
    15901 aacgatgctg gaagtagaag gtgtaaacgg caatatcgat attcgaaacg ggaacatctt
    15961 agattctatt atcgaaacag taaatggtac agtgacattt ggtgcgacag cagaaaacct
    16021 ttctgtgtct ttggtaaatg gtgatgtacg tcttacgatt aaagaagata atttgaaaaa
    16081 agtagaagca agttcagtga acggaaatgt caaagtcgct ttaccagatg gcatcggttt
    16141 ggaaggacat gctaaaacaa gtcttggaag tatcaacagc cgtctgtcca attacgaagt
    16201 agttcgtgag aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg
    16261 tgaaatcgcg caagtgcaac tttcaacgac aacaggaagt atctatttaa aagatacaga
    16321 taaataaaga ggttgtgaaa gagatgttca gccttgagca ataagaagcg attttgtaaa
    16381 atagcttccc ctattttaca aaattgtgcc ttattgccga tagtctatct cttgaacacc
    16441 gtttaccaag agattatgaa cctgccaatt atcgccgagt cacaaggaat aatttcagaa
    16501 aaagctcgtc acgttttatg aatatggtaa tttgtgaccg agaattttgc agaaagacac
    16561 cgattaagaa gaaggttgtc acaacgattt tcttcattta aaatagtaga acagaggtga
    16621 gacatgatga aaaaaaagtt gacaaaatct aataaaaatg ttgttttgac agggacttta
    16681 gcaggaatag gagaatacat cgggattgat ccgactgtta tccgtgtgct ttatgtattc
    16741 ctgagcttag ttgcttttgg aagtccaatt attttataca ttttattagc tttgattatc
    16801 ccatctgcgc gttctgcacg aacaaactat ggacataaca atgcatatta tgaaaaaaac
    16861 aattatcgtg aacaaactcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
    16921 gatgacgaat ggagtgactt ttaatgtcgt actttcaacg tattttagtt aatacgttaa
    16981 cattcatatc attggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
    17041 ccgttcttgc cagcttcgta ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga
    17101 tactgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
    17161 cgattcttaa aatgacctcc ttttttgtgg gagcagaaca ttttagtttt tcttcattcg
    17221 gtgcagcgtt gttagtagct atcttgatgt cgcttgtcaa tgtaattgtc agcgaacata
    17281 acagtagaaa ataaaagaaa gctgtgccga actatcggcg cagttttttt atttagaatc
    17341 aggaatgcgt tttctattac attggtaatt aaataaaaac gtttttttta attggtagta
    17401 cttttggcta gacaatgata aaatgataga gaacactcta atagaaatga ggtagcatga
    17461 atgtcagaag ttgtaaaagt tagccaatta gtgaaaaaat tatctttgga aatcgtgaca
    17521 ggcgacgaga aaagcttaga ccgtgtcatc gtaactgggg acatctcacg cccaggacta
    17581 gagttgactg gttattttaa ttattattcc cataatcgtt tacagctatt tggcagtaaa
    17641 gaaatcagtt ttgcggaacg gatgatccca gaagagcgtt tgatcatcat gagaagatta
    17701 tgtagtgaag atatgcccgc atttattatt tcaagaggat taccagctcc agaagaaatg
    17761 atccaggcag caaatgaaaa tgggatagct gtactgcgtt ctcccatctc aacttctcgt
    17821 ttattaggag aattatccag ttatttagat ggtcgccttg cccctcgaac aagtgtccac
    17881 ggtgttttag tggatgttta cggattaggt gtactgattc aaggtgacag tgggatagga
    17941 aaaagtgaaa cagcgttgga gttgatcaaa cgcggccatc gattgatcgc agacgatcga
    18001 gtagatgttt atcagcaaga tgaattaacg gtgatcggag aaccgcctaa aatcctggaa
    18061 catctgattg aaatccgagg aatcgggatc attgatgtaa tgaatctttt tggtgcgagt
    18121 gcggtacgtg gctctatgca agtccagttg gctgtttatc tggaagcatg ggcaaaagat
    18181 aagaaatacg accgtctggg ctctgaagac acgaccgttg aaatcgcgga agtgggcatt
    18241 ccacaaataa aaatccctgt caaaacagga cgaaacgttg caatcatcat tgaagtagcc
    18301 gcgatgaatt tccgcgcacg tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga
    18361 ttaagtcgct tgattgaaga aaactcaggc aacgactagc aaggagtgac aatatgttag
    18421 cacaaatcaa tcgagttgct tttgagcttg tagggattcc tatctactgg tatgcattga
    18481 tcatcgtatc aggtatcatt atcgctatgt ggcttagcag cagagaggct gttcgggtag
    18541 gattaaaagc agatgatgtg acagatttta tgttgtgggg attgcctatc tccattattg
    18601 gcgctcgctt atattatgtt ttatttgatc ttccccagta tatagctgat ccaatccaaa
    18661 tattcaatat ccgttcagga ggcttagcta tttatggcgg actgattgca ggcgcgatca
    18721 cgctgtattt ttttactaaa tatcatttta tttctttgtg gactttttta gatatcgctg
    18781 caccaagtgt attattagca caggcaatcg gtcgttgggg aaatttcatg aaccacgaag
    18841 catatggacc agacacgacg cgaagcttct tagaaagtct gcatttaccg caattcatca
    18901 ttgataatat gtatatcgaa gggatctatc gtcaaccaac atttctttat gaatcacttt
    18961 ggagtttgat tggatttatt ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa
    19021 aaggcgaagt ccttttatgt tatctaatgt ggtatagttt cgggcgtttc tttattgaag
    19081 gattacgtac agacagtctt tactttttag gtgtgatccg tatttctcaa ttattatctg
    19141 ctgtgttatt tgtcggtaca ttgattctat ttatttggcg cagaaaaaaa ggaaatttac
    19201 cggattatga ccgctcatta ggaaaaaatc aacaaattat ctgattttta gagcgttaaa
    19261 aatgaaaaaa atttcaggag tggtaaaatg aattatagag tagcggtttt aggaccaggc
    19321 tcttggggta ctgctttggc acaagtcctc gccgaaaacg gacatgacgt tcggatttgg
    19381 ggacatcgtg cttcacaagt agaagaaatc aacacccaac acacgaatca acgttattta
    19441 ccagatcatt atctacccga ttccatccaa gcattcacca atatggaaga tgcagtaaaa
    19501 gacgcagatg cggtgctatt cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa
    19561 ctagtgccta aattgaatac aaagccagtg atcatccatg caagtaaagg attagaacag
    19621 ggaacacata aaaggatctc tgacatcttg ttagaggaaa tcccagcaga aaaaagaaaa
    19681 gctgttgttg ttctttctgg accaagccat gcagaagaag tagcaaaaca tgacatcact
    19741 acgatcacag ctgcaagtac agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg
    19801 aataattatt ttcggatcta taccaatcct gatgtgatcg gtgtagagat gggggcagct
    19861 ttgaaaaata ttattgctat tggtgctggc gctattcacg gcttagggtt tggcgataat
    19921 gcaaaggcag caatcatgac gcgcggatta gctgagatca gccgtttagg tgtagcaatg
    19981 ggtgccaacc cgctgacctt tatcggatta agcggaatag gcgatctagt cgttacttgc
    20041 acaagtgttc attctaggaa ctggcgcgca ggcaacttgt taggacaagg gcagccccta
    20101 gaagaggtgc tagccaatat gggaatggtc gttgaaggtg tgaatactac ccaagcagcg
    20161 atggaactag cccaaatgat ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg
    20221 ctgtatgaac aaaaagaaat caaaacagcc gcaaaagaaa tcatgttacg tgatggaaaa
    20281 aaagaaaatg aattctcatt aacaattttt tgaaggagcg aatgtatatg aaagttaaaa
    20341 aagcagttat tccagcagca ggattaggaa caagattttt accagcaaca aaagctatgg
    20401 caaaggaaat gttgccaatt gttgataaac caacaatcca gttcatcgtt gaagaagcgt
    20461 tgaaatcagg tatcgaagac atcttgatcg ttacaggaaa agctaaacga ccaatcgaag
    20521 atcactttga ttcaaatatt gaattagaaa caaacctaag tgaaaaagga aagaccgaat
    20581 tattgaaatt agtagaagaa acgacagatg tcaacttgca tttcatccgt caatctcatc
    20641 caaagggttt aggacatgcc gttcttcaag caagagcctt tattggaaat gaaccatttg
    20701 tcgttatgct aggcgatgat ataatggaag acgaagtacc attgacacag caattaatcg
    20761 atgattacga acgtactcat gcttctacga tcgccgttat gcaagttcct catgatgaaa
    20821 cttcaaaata cggaattatc aatccaggag aagtattgga agatgggtta tacaatgtca
    20881 aaaactttgt tgaaaaacca gatccaagca aagcaccaag tgatttagct attattggac
    20941 gttacttatt gactccagaa atttttgatg tattgtccga gcaacaacca ggagccggaa
    21001 atgaaatcca actaactgac gcaatcgata cattgaataa aacacaacgc gtatttgccc
    21061 gtaaattcac aggtaaacgt tacgatgtcg gtgacaaatt tggttttatg aaaacaagta
    21121 ttgaatatgg tctaacacat ccagaagtag gtgcaccttt acgtgagtac atcctgtctt
    21181 taggtaaaac attagaagca gaaggaagca agatcgatca taatccaaaa ggaataacag
    21241 aaatcaaaga agaaaagaaa aaataaccct tttttctaac gagcttcagc tgctaattga
    21301 ataaaaagta gttgaagctc gtttttattt gtttaaaaaa cacgaaaatg ctatccttat
    21361 agaaaagaga gacaagaagg gagtttgatc atgtcaggag gagaaattgc aggattgatc
    21421 gcagcaatcg cctttgcgat acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct
    21481 aaaactttag aaaaagtaga tcagactgta gcagaagcaa atacgacgat cgaaatcgtc
    21541 acaaaagacg tagatctttt gtctagacaa gtagaaggct tactggtcaa aagtaatgaa
    21601 ctgctcttgg atatcaatgg aaaagtcgca acgatcgatc cattgttcac agcagttgct
    21661 gatttaagtg aaagtgtatc agaacttaat cattctagta aaaacatcgc gacgaaagtc
    21721 ggcggaatcg gtaaaacgac agctaaagcg acggttgccg gaaaagtcgg tgggacagcg
    21781 atgaaatttt ttaaaaacaa aaaacatact gaaacgacag gaggaaaata aaaatggcta
    21841 aaaaaggtgg atttttatta ggtgcagtag tcggaggcac agccgcagca atcgccgcgt
    21901 tattgttagc accaaaatct ggtaaagaac ttcgggatga attagctgcc caagcggatg
    21961 atttgatgga tatggcttca gattacacag atatggcgaa agaaaaatcg actgagttaa
    22021 caggtattgc aaaagaaaaa gcgcaagatc tttctcaaca agcaggtgat ctagcaggta
    22081 gtgtcaaaca aaaagtaagt gaatcagcag atgaagcagg aagtgaatct gacgatatct
    22141 tgttaaatct taaagaacaa tcagctgact tatctaatcg tttcaaaaaa acggcagaag
    22201 atatgaaaaa acctgcagat gaattaggaa gtattgctaa agatacttca gaagatatca
    22261 tgattgatgt aaaagacgct gctggtaaag caggagatac tgtcaatgaa ggaatgtcag
    22321 aagcaaaacc agttgcagat gactcaaaag aaatgatgaa aatggcaaaa gaagatgcga
    22381 aagaagctaa aaaagaaata aaagatacaa tgacagattc atctacaaat cactaaaatc
    22441 acaaatttat agaataaaaa ggtgggagcg gactagctct cacctttttt actcttattt
    22501 ttctaaaaca agtaattctt ttggtgtagt cgtgaatggc acacagccat cgcttgtcac
    22561 atatacacag tcttcgatac gtacccctac tttttcagga atatagattc ctggttcaag
    22621 agagaagcac atcccttctt cgattactaa gtcatttcct tctacaaggg aagggaattc
    22681 gtggacggtt gttccgatcc cgtgacctaa acggtgattg aaatattcgc cgtagccata
    22741 gctgttgatc acgtttcgtg cgatttgatc aagttcacct gctgtcacac ctggacggac
    22801 ggcttcttgt gcggcaagct gtgcttcaag agtgatgttg tagatttttt cttggaagtc
    22861 ggttggtttt tgataagcaa ttgtacgagt agcatcactg caatagccat tccaaacaac
    22921 gccaagatcg aataacacaa gttcattcgg tgaaatcttc gtatttcctg gtgtaccatg
    22981 agggcttgca gcatttgctc ctgcaaggac taatgtgtcg aaagacattt gtgaaacacc
    23041 ttgttttttc aattggtatt caatttctgc aatgatcgct tgttcagtca cgccttcttt
    23101 cactgcttta aagccaatct caaatgcaac atcggcccat ttaccagctt cgatcagttt
    23161 gtctttttcc tctagagttt tgatcagttg tagacgttgg atgatggaag ttagatcagt
    23221 gtaagaatcc gttcccgtca agatatcaga gatagcatcg aagcgctcga cagtcaaggc
    23281 gttcttttca atccctacat tgccaggctg accatagcga gcacgaagtt cttttccgat
    23341 gatctcccaa gggttttcgc tgtctaaata gccacggaca tcatacgccc aactactgtt
    23401 ttgagcatct tcaacttcca aagcaggagt gaaaaggaaa ggatcttgtt caagagggat
    23461 aaaaagagct agtacacgct catgaggatc actttcgtat ccggaaaagt aagcgatgtg
    23521 gccagggtcg ctgatataag ccaattcgac ttggtgttgt gccatccatt gacgtagttc
    23581 attgattttt acgttattca tttggcaacc tactttctaa attaattttg tattcaataa
    23641 ttgtaccact gatttattaa aagaccaacg ttctaaaaac tgaaaaaagt aaaaattttc
    23701 atgaaaacag agataaaacg gttgcaattc ttgagattat ttgatattct attttagaaa
    23761 tgtaagacgt attttcagaa aacatataaa atatgaatag gagaacacaa atggaaaagc
    23821 aaacaatcac tatctatgat gtagcgagag aggcaaatgt ttcgatggct actgtttcac
    23881 gtgtcgtaaa tggcaatcca aacgttaaac cagcaacaag aaaaaaagtc ttggaagtta
    23941 tcgatcgctt ggattatcgt ccaaacgcag ttgcgcgtgg cttggcaagt aagaaaacaa
    24001 caacagttgg tgtaatcatc cctgatgtca gcaatatgtt ctttgcttct ttagcacgtg
    24061 gtatcgatga tgtggcaaca atgtacaaat acaatattat cttagcaaat tcagatggca
    24121 acgaccaaaa agaagtcaat gtattgaata acttactggc taagcaagtg gacggcgtga
    24181 tcttcatggg tcaccatatc acagacgaaa tccgtggaga attttcacgt tcaaaaactc
    24241 ctgttgtatt agctggctct attgatccag atgaacaagt aggtagcgta aacattgatt
    24301 atgtatccgc aacaaaagat gcagtaaaca aattggcaaa aagcggcaat aaaaaaatcg
    24361 cttttgtcag tggtgcgttg atcgatccca tcaatggaca aaatcgtctg aaaggctata
    24421 aagaagcatt aagcgaaaat aacctaccat atagcgaagg attgatcttt gaagcacaat
    24481 acaacttcaa agatggtcta tctctagtcg atcggatcca taatagcggt gcaacagctg
    24541 tctatgtttc agatgatgaa ttagccatcg gtattttgga tggtttatta gaccgtggcg
    24601 tgaaagtacc ggaagaattc gaaatcatca caagtaacaa ttcactactg acagaagtag
    24661 ctcgtcctcg tttaacaagt atcacacaac cattatacga tttaggggcg gtatctatgc
    24721 gcctattgac gaaaatgatg aataaagaag aagtcgaaga aaaaacgatt atcttgcctt
    24781 atggaatcga agaaaaagga tctacaaaat aataaaagga caaccattca gaggttgtgt
    24841 gcctgccgtt taactctgcg ttacaaggag caatttcaaa aaacagcatc tcatgtttga
    24901 tgaatattgt gactcgtgat tcggagttgg caggagaaca ccgtttattt gggaaaaggg
    24961 gttatgacaa aatttt
//
LOCUS       DAZULO010000031        24474 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000031 DAZULO010000000
VERSION     DAZULO010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.032"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..893)
                     /locus_tag="ACG8OA_001281"
     CDS             complement(45..893)
                     /locus_tag="ACG8OA_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4976079.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1117..3057)
                     /gene="thrS"
                     /locus_tag="ACG8OA_001282"
     CDS             complement(1117..3057)
                     /gene="thrS"
                     /locus_tag="ACG8OA_001282"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFS4976080.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3394..3573)
                     /locus_tag="ACG8OA_001283"
     CDS             complement(3394..3573)
                     /locus_tag="ACG8OA_001283"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFS4976081.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3620..5443)
                     /locus_tag="ACG8OA_001284"
     CDS             complement(3620..5443)
                     /locus_tag="ACG8OA_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4976082.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5721..8000
                     /gene="helD"
                     /locus_tag="ACG8OA_001285"
     CDS             5721..8000
                     /gene="helD"
                     /locus_tag="ACG8OA_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFS4976083.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8093..8740)
                     /locus_tag="ACG8OA_001286"
     CDS             complement(8093..8740)
                     /locus_tag="ACG8OA_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976084.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8919..9638)
                     /locus_tag="ACG8OA_001287"
     CDS             complement(8919..9638)
                     /locus_tag="ACG8OA_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4976085.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9769..10920)
                     /locus_tag="ACG8OA_001288"
     CDS             complement(9769..10920)
                     /locus_tag="ACG8OA_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFS4976086.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11137..11877)
                     /locus_tag="ACG8OA_001289"
     CDS             complement(11137..11877)
                     /locus_tag="ACG8OA_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4976087.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11881..12204)
                     /gene="rsfS"
                     /locus_tag="ACG8OA_001290"
     CDS             complement(11881..12204)
                     /gene="rsfS"
                     /locus_tag="ACG8OA_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFS4976088.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12261..12854)
                     /gene="yqeK"
                     /locus_tag="ACG8OA_001291"
     CDS             complement(12261..12854)
                     /gene="yqeK"
                     /locus_tag="ACG8OA_001291"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFS4976089.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12838..13488)
                     /locus_tag="ACG8OA_001292"
     CDS             complement(12838..13488)
                     /locus_tag="ACG8OA_001292"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFS4976090.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13498..13809)
                     /gene="yhbY"
                     /locus_tag="ACG8OA_001293"
     CDS             complement(13498..13809)
                     /gene="yhbY"
                     /locus_tag="ACG8OA_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFS4976091.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13825..14934)
                     /gene="yqeH"
                     /locus_tag="ACG8OA_001294"
     CDS             complement(13825..14934)
                     /gene="yqeH"
                     /locus_tag="ACG8OA_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFS4976092.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14931..15458)
                     /locus_tag="ACG8OA_001295"
     CDS             complement(14931..15458)
                     /locus_tag="ACG8OA_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFS4976093.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15638..16423)
                     /locus_tag="ACG8OA_001296"
     CDS             complement(15638..16423)
                     /locus_tag="ACG8OA_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFS4976094.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16416..17285)
                     /gene="accD"
                     /locus_tag="ACG8OA_001297"
     CDS             complement(16416..17285)
                     /gene="accD"
                     /locus_tag="ACG8OA_001297"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFS4976095.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17286..18665)
                     /locus_tag="ACG8OA_001298"
     CDS             complement(17286..18665)
                     /locus_tag="ACG8OA_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFS4976096.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18673..19092)
                     /gene="fabZ"
                     /locus_tag="ACG8OA_001299"
     CDS             complement(18673..19092)
                     /gene="fabZ"
                     /locus_tag="ACG8OA_001299"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS4976097.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19117..19593)
                     /gene="accB"
                     /locus_tag="ACG8OA_001300"
     CDS             complement(19117..19593)
                     /gene="accB"
                     /locus_tag="ACG8OA_001300"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFS4976098.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19596..20831)
                     /gene="fabF"
                     /locus_tag="ACG8OA_001301"
     CDS             complement(19596..20831)
                     /gene="fabF"
                     /locus_tag="ACG8OA_001301"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFS4976099.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20850..21587)
                     /gene="fabG"
                     /locus_tag="ACG8OA_001302"
     CDS             complement(20850..21587)
                     /gene="fabG"
                     /locus_tag="ACG8OA_001302"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFS4976100.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21599..22516)
                     /gene="fabD"
                     /locus_tag="ACG8OA_001303"
     CDS             complement(21599..22516)
                     /gene="fabD"
                     /locus_tag="ACG8OA_001303"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS4976101.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22623..22847)
                     /locus_tag="ACG8OA_001304"
     CDS             complement(22623..22847)
                     /locus_tag="ACG8OA_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS4976102.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22900..23862)
                     /locus_tag="ACG8OA_001305"
     CDS             complement(22900..23862)
                     /locus_tag="ACG8OA_001305"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFS4976103.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23872..24312)
                     /locus_tag="ACG8OA_001306"
     CDS             complement(23872..24312)
                     /locus_tag="ACG8OA_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4976104.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6703 a         5441 c         4254 g         8076 t
ORIGIN      
        1 cgttttgtta ttctaaaaaa cttatataaa aaatagtaag gtttttattt cgaatccata
       61 aaatattctt ggattgcttc tcgtgttttt ttcacttttt cgctaagctt ttcgttttta
      121 gccatataga aataaaaaat gatatcgatc gtagcaaatt gggccaaaag tgaattagtt
      181 gccgcacttc gattcgtgat ttcttttggt ctagatgttt gtacgagtac atcggctatc
      241 ttgctaagcg ggttatttcc aaattgcgtc aacgcaatga tttcgatatt catgcttttc
      301 gccagctcgg ctaatgcgac tacatctgca cttctacctg agtttgaaac aagccaaagc
      361 actttctttt tctcattgct gacaagttgc gggagcaaga cgtggatatc tttttcaaaa
      421 atgattggct taccgacacg agaccatttt tgcagaatat cttctactac taaagaagag
      481 gcgccaaccc catataaaaa aacagtctct gccgattcca aacactctac gactttttgg
      541 atcgtttctt cttctagaat atctactgtt tcttttatcg ctacttgtgc attgttacca
      601 agtttgtttt tgatagagta aaaggactca tttgcttcta tatcactaaa accttcatcg
      661 attccagtct gtagttctgt tgaaatcgtg attttgaagt ccgtcagact ttgaaaaccg
      721 atttttttgg aaaaacgtac aactgtcggc gcactcaacc cggaagcttc tgctaattca
      781 ttagctgtca ttgtagggac cttctcaata ttaccgataa tgtagttggc caactttttt
      841 tctgctgaag aataatcttc catcatactt tcaatacgac taattacgct cataagttcc
      901 tccacccttt taatgatagt ctatgtaagt ataccatatc agacccttat tcacggtcgt
      961 atactggata aaaaacaaaa aaacaatccg cccaacagaa tatcttatcc catcaatccc
     1021 acagattcta ttgagcatct gtgtctttga ggaatacctg ttatctattg ttcggattgc
     1081 tttctatcta ctactttttt acaggaaatt tacctattag ttttctctgc tgtaattttt
     1141 gacttctgct tccatagcat caataaagat attcaaatct tctacagctg tttctttact
     1201 tccataacga cgtacgttca ctgttgcatc ttccaattcc ttgtctccaa caactaattg
     1261 gtaagggatc ttttgtgttt gagaggcacg tatcttatag cccatttttt cattacgatc
     1321 atcaacttct acacgcaacc ccttcatttg caaacgttct ttgatttcat aagcataatc
     1381 gccatgtgca tcaacagaaa cagggatgat tgttgcttgt ataggtgcca accatgttgg
     1441 aaaagcgcct ttgtatactt ctgttaaata agcgacaaaa cgttccattg tagaaacaat
     1501 tccgcggtgg ataactactg gacgatgcgt attttccccg tcttctccaa catatgtcaa
     1561 atcaaagcgc tctggcaata agaagtctaa ttgtatagtt gataatgttt cttctgtacc
     1621 aagagctgtt ttgacttgaa catctagttt tggtccgtaa aaggctgcct cgccttctgc
     1681 ttcaaagtaa tctagttcta attcatccat agcggctttc agcattgctt gggctttttc
     1741 ccacattgcg tcatcatcaa aatatttatc tgtattgttt ggatcgcgat agctcaagcg
     1801 gaaacgataa tctgtaatat tgaagtcagc atatactgcc accatcaatt caagcgtacg
     1861 tttaaattca tctttgattt gatcaggacg aacaaatgta tgaccatcat tcaacgtcat
     1921 ttctcgtaca cgttgtaaac ctgacaaagc acctgatttt tcatagcgat gcatcattcc
     1981 taattcagcg atacgaattg gtaattcacg ataagaatgg atcgtgtttt tgtagaccat
     2041 catgtgatga gggcagttca ttgggcgaag aacaagcatt tcgccatctc ccatatccat
     2101 tggcgggaac atgtcttctt gataatgatc ccagtgtcca gatgttttgt acaactcaac
     2161 atctcccatt attggtgtat atacatgttg gtaacctaga ctaacttctt tgtctacgat
     2221 ataacgttcg atcgtgcgtc ggattgttgc cccttttgga agccagaatg gcaaaccaga
     2281 acctacttct ggggaaacca tgaacaagtc aagttctttt cctagtttac gatggtctct
     2341 ttcttttgct tcttcacgga gacggatata ttctttcaaa gctttcttgt caaaaaaggc
     2401 tgtcccgtat acacgttgca tcatttgatt gtttgagttt ccacgccaat aagcacctgc
     2461 cactgataat aatttaaata cttgtatacg gccagtggat ggaacatgtg gtccacgaca
     2521 caaatcgatg aattcgcctt gttgataagc agtaatcact tcttcttctg gcaattcact
     2581 aatcagttct actttgtacg gatcacttgc aaaaatttct aatgcttcct gttttgaaag
     2641 aacacggcgt tcgatcggca gattttcttt gacgattttc atcatttctg cttcgattgc
     2701 cggcaaatct tctgcagtta cttggttttg tccattatct gtatcgtagt aaaaccctga
     2761 atcgatagct ggtccaacgc caaagtggat atttggatag aggcgacgca tggcttgggc
     2821 catcaaatga gctgctgaat gacggactaa gccaagtgca tcttcatggt ctggtgtgat
     2881 gatctcaata cttgcatctt cctcgattgg acgagtcaaa tcaacgagtt ttccgttcac
     2941 tttaccagct aaagcttttt ttgctaagct ttttgaaatg ctttcagcaa ttgccaatgt
     3001 tgttgttcca gactcaaatt ctttgactgc accatctgga aaagtaattt ttatcataat
     3061 agtttcctcc ttgaaatgtt ttgattgatc agatcatcaa gctgaaaaac aaaaaaatcc
     3121 tagcatctct tgaaaagaga tactaggacg aaaagttcgt ggttccacct aatgttcaaa
     3181 cgcaaatgcg ttcctcaagc gttgtaacgg tccgaccgcc cttgcttcaa caagggtttc
     3241 gaaagtggtc atcctttggt tttctcctgg aaaagtttca gcctagcttt tcctctctta
     3301 tcgaagactc tcaaattcag ttggctttct catcaatcaa ttatgtgttt atttaaccac
     3361 taataaatag aaaaatcaag tgggaaacta aacttatttt ttccattccc catttacacc
     3421 gtatgttcct tttttcatct cattgatgat gacatggatg tgttcttttg gtgcctgcgt
     3481 gtttttatga acagcttccg tgatatcttt catcatattt tctaattgtt ccggactgcg
     3541 accttctact aattcgacat gtacgaatgg cataatgctc tctcctcttt ctgatcgttc
     3601 ttttttcaac tcgattctat catactatgt ttgtcttggg tactgaatca ggaaaaatgg
     3661 ctggatcttt ttttgctctc aaggcactca aaatagagac cgtatccacg acttcttgca
     3721 aaacagctcc gaataatgcg gggattagtc ccgtacttgc aattaacatc aaaaaaatac
     3781 aaataaagat gccaatcagt acagactgtt tcgctattct catcgtatct cttgaaatca
     3841 ctactgcttt tgctacacgc aagagatcat ctttcaagat gaccgcatct gcactttcac
     3901 tagcagctgt agaaccatga gcccccattg caataccgat atctgcagca gcaagtgctg
     3961 gtgcgtcatt cacgccgtct cctaccatcg caacaggtct ttgatcttta gggatacttt
     4021 ctaaaatggt cagtttatct tgaggcaagc tacgggcatg tacagaatct aattggactt
     4081 tttctgcaat cgtttgagca atggcagatc catccccggt gatcatcatc aaatttttca
     4141 catcaagttt tcttagctcc ttgattgtct ttgctgcttc tgggcgaatc tgatccgaaa
     4201 ataggatcgt tcctgcaaaa gatcgatcaa ttgttacata gattgctgta ttggacgttt
     4261 ctatttcttc agctgaaaca aaagaggcac gtccaatctt tacttcatga ttattgacta
     4321 ctgcttggat tccttgaccg gtcacttctt tcagttgact aacaggatac agttggctgt
     4381 ttttcacgtt gttcactaac gaacgagcta agatatggct agattcctgt tctgcactag
     4441 ctgcatacag aagtagctct tcttttttta ttccattgac tggctgaatc gcctctactt
     4501 ccaatatccc tttggtaagc gttcctgttt tatcaaaagc aatcgttttt gtacgtgcga
     4561 gcttttcaat cgttgttcca gtttttacaa cgatcccgtt tttacttgaa cggctcattc
     4621 ctgcaactaa agcaatgggc gcagctagaa tcagtggaca aggtgatgcc actaccagta
     4681 cctcagcaaa tcgtaaaggg tcttttgaga accaccaaga aataccaccg atcaaataag
     4741 caatcaacgt gaaagggact gcataacgat cagctaaacg gacaaatcgg gcaggctttt
     4801 cttttgattc ttcgactagt ttaataatca tttgatactg gctgtctgcg gcacgttttt
     4861 cagcgatcat ttggatcgat ccgtctccat ttaaagaccc tgacataatc tgatctccag
     4921 gtttcttggt ttccgggcgg gactcacctg tcaaagaaga ttcatcgacc gaagattctc
     4981 ctttggtcac tcgaccatct gcgggtacaa gttcgccagg cttgactagt aactggtcat
     5041 caatatttat ttcatcgatt actatatcct gcaattttcc ttctttgaat cgatgggcaa
     5101 ttcttggggt gttatctaac aaagactgca gttctttacc cgctcgttta ttcgcgtaat
     5161 cctctagact atctccgccc gtaagcatga tcaatatcat caaactagcc cagtattctt
     5221 tgacggctaa tgttgcaagg attgcggtaa tcgctaatac atctacgcca tatcttcctg
     5281 aacgtaacgt cttgatcatt tccaaaaaca tcaagaaggc gagaatccct ccagtaaatg
     5341 cgacaatcgc aaatgcaagg gccggctggt gaaaaacaaa ttcacttaat aaagctatcc
     5401 caccaataag caaagtaagt aaaaattttt tgaaatgtgt cattgttttt tctcctcttt
     5461 tatgtgactt agagaggtta gtaataaatt tccagctgcc tctttcatct taagcatagt
     5521 ccttgactag cactttttaa aacaataggc cttaattgaa aacaactatc actatcagcg
     5581 tttccaaaat tcatcagttc tacaaacaaa aatattcagc aaggattact ttttcgattc
     5641 atcagttaaa aagtaccttt tttcgaaaaa ctatgctata cttttaattc gttatttacg
     5701 gcggaaggag caaacatgcg atgaatgaga aacaactaga acaaacgcac gtggatgaaa
     5761 cgatcgaact aatccagatc gaacaaaata tgctggatac aaaacaacaa gaattgactc
     5821 aggaaatgaa tcattcgaca aaagacacag ctaatcacaa gattcgagct ggttccaatg
     5881 aagcttttta tgaatctgct gttgaatatc gccaacatga gcaggaattg cttttgaaat
     5941 accatactct tgaagcacag caaaaacgcc tgcaaacgtt gtctgtcatg aaagatagtc
     6001 cttattttgc ccgtatcgac tttaaagaag accgtgaaac agaaacatta tatctaggta
     6061 tcgcttcttt aagagatctg gcagaagaaa caatcgttat tgactggcgc gcaccaattg
     6121 ccaatcttta ttatgaaggt gagattggtc ccgcatttta tgaaactgaa acagatagaa
     6181 tagatgtcga tctgcagttg aaacgacaat ttaaaatcca agaaggacgt attttatcta
     6241 tggtagatac gtcggaagta atcaatgatg actttttgct agagatattg gatgaatcat
     6301 caagcaacca tatgaaaaac atcgtctcta cgatacaaaa agcacaaaat gcaatcattc
     6361 gggacacaac aagtaaagta atgctgattg aaggaatcgc cggaagtgga aagacatccg
     6421 ctttgcttca acgtattgct tttctgttgt atcataatcg caagtggctc gaggctgaaa
     6481 acgttcttct attttcaccc aaccatttgt tttctgatta catctcgact gtattgcctt
     6541 cattaggtga aagcggcgtg cctacacaga cattcaaaaa ttatttggtg cagctgctgc
     6601 cagagttttc tttactagat gaacagcaac aagaaagtgg ttttttgcta ggggaaaaag
     6661 atccgataca agtgatgaaa agcggattga ccttagtcga tcagattgat cgctatatcc
     6721 aaggcatcac aaactacggt cccttgttcc gtgatatgaa aataaatggc cgtacgatcc
     6781 tatcaaagga atcgattcgc caatggtata aagaaacaaa tgaacaatta cctttacatc
     6841 atagactttc tctattacaa acgaagctcc tgaaaaaatt aggcggtctg caaaaagacg
     6901 aaacacgtca gcaatgggtc aaagatctgg cggaagaaca gctccaagaa ctatacgcaa
     6961 cagatccaaa tcttgaatat acagaacaaa aggagcgttc attacgtaaa aaactggcca
     7021 accaaatcgt acgtaaaaga ttccgtcgaa tccatcgagg gatcatgaat tatcaatttg
     7081 tcaatataac gaaacaatat ctccactttc tacaaactgt gccactttct cttctaacac
     7141 gacataaaat cagcgagacc aggtggcagg aacatttgac aactgttcgt acacacttgc
     7201 gtcaaagaga attgcgtcaa gaggatgctg tactattctt tttactgatg cgccgaacat
     7261 atccagtgac ggttacacac aaagctcgct atatctttat tgatgaaatg caggatttcg
     7321 caccatcaca agtggcattg ctgcaagcct tgttcccaaa agcaaatttg actttctgtg
     7381 gagatctaaa tcaaaatgtc tttggaaatg aaacgatcgt tggatcattg gaagaccttt
     7441 tcgtggaccg tgaagtcact cgtttccaat taacgacaag ctatcgctcc acaaaagaaa
     7501 tcactgattt tgctaatcat tttattgtga aagaacatca agtagaaaca acagcaagaa
     7561 aaggagacct tcctttgctc atccaaggaa caacagatga tatcaaattg aattggttag
     7621 cagataccat tcgaacgact cagggaaaag cccggtattg gcgtacagca atcatagcta
     7681 aaacacaaga agaatgcgaa gctctttatg ctcaactgcc agacgagttg aaacaacaag
     7741 tccagctgat tttaaatgag agtgacttta tgaagcgttc cattatttta cttccagcct
     7801 ttttagcaaa aggtctggag tttgaccgag ttttcttgtg ggatgtagat gcagaacatt
     7861 tttcttctca gcaagacaaa ttgatccttt acaccatgtg tacacgtgcg atgcatgaat
     7921 tgaccctttt tacctccaat aatgaaagtc ctttgttaaa caatatggat atttcatctt
     7981 atgagaaaaa agtactgtaa gtaaaaagac aactgctatc attaatgtta gcagttgtct
     8041 ttttatttac aataataatt gcagaaaaag taattcaatc atttaaggtc atttattcta
     8101 tatataaatc caaatctgtt tcaggatatt ttaatttgac aagcgaaata atcattttca
     8161 gttcttccat gttttctgga tattctgggt caaatacctt gctatgacgg ccaatcccgc
     8221 cattgtcgat caatcggtaa attcgattcc attcaatgaa ttggtcaatt gattggaaat
     8281 tgctgattgg atagccgcca caatccaaaa tctctgatac acgaaatgct aggtatgcac
     8341 tatcgatcac tttatttttt ttcgtgtcta tcttctcatt tctttcgata agaatttttt
     8401 caggacgatg tttcaagctc cactctaata gtagaaagaa agcctccatc cgatactctt
     8461 cttcaaataa ccgaaaggct gaatcagaac gacgaaaaag cagcggaatc agataatttt
     8521 gtctataaat cagcattggt gcgcggtcca ccatacactc taaataacgg ttcagatacc
     8581 ctgtaccgac atagtttcct aatcgatagc cttcaacgcc gaatcttgcg tatttttgcg
     8641 ctaattttga acaatcagtt acgttcacag ctaactcttc tactttgcgg taaccgctta
     8701 agatacgttc aaaaggttct aaagtgattt ttgcttccat attcttcccc gtccatcttc
     8761 cacatacttc atatcatttc acatgatact tatgctctca tcataccaca ggttgaatta
     8821 gaaaagaaaa taaatcacac aaaaaagcaa gacaatctgt tagtgagatt gtcttgcttt
     8881 tcgaaaaaga ctgaatactt ttcgttaaaa ctggttgctt agtcttctaa tgccacatta
     8941 tggaacacat gctgtacatc ttctagatct tccagtgcat caatcatttt ttcaaatgtt
     9001 tcttgatctt ctggagatag tgttacctcg ttttgaggga tcatttggat ctcagcgatt
     9061 gaaaaatcag tgattccttt ttcttttaat gcttcttgta tactatggaa atccgctact
     9121 tcaccgtaaa cgatgatttg tccttcttct tccattacat cgcgaacatc gatgtctttt
     9181 tccattaggt attcaaggat ctcgtcagcg tcttctcctg caaaagcaaa gattgctgta
     9241 ttgtcaaaca tgtaagaaac cgctccggaa acacccatgt tcccgccatt tttcccaaat
     9301 gctgcccgta cgtctgctgc tgttcgattc acattgttcg tcaatgtatc aacaatcacc
     9361 attgagccgc ttggtccaaa tccttcatag cgcaattcag agtatgtttc atctcctgca
     9421 ccttttgctt tttcgattgc tcggtcaata atatgttttg gtacattata agttttagcg
     9481 cgatcaatga cgaaacgtag tttttggttt gcatgcggat caggatcacc tgattttgct
     9541 gccgcgtaga tttcaatccc aaattttgca taaatccggc tattattagc atctttagca
     9601 gttttctttg ctacgatatt tgcccattta cgtcccatat tttcacttcg ctttctgatt
     9661 tgatagtttc ataacgcaaa aacgtcctgt tccattatac ttgtgaatca cgattctgtg
     9721 aagctttttt tctttcttcc ttcattcttc tcatttttag tcaattatct atactcggat
     9781 tgggatttgc ccgaaattct gttccggaat attgcctccc aattgataga tttgatcact
     9841 tctaactaac agttccgctt cttgtgcctc tttttttccg attcgggaat aaatagaaat
     9901 ctcttttttt tcttttaaga atcttcttcc agcatcggta aatccaagta gtcgaagata
     9961 atttttattt tgttggacag gaatctcttg ctttttgata ttcagcaaga cataacaaga
    10021 taatcgttgg atccgtgtcc atgtatatct ttttgttttt acctttgaca caaaatcctc
    10081 aaaactatca gcagttgcag ctgctgtttt catgcgcgat tctattcctt cggtcatctg
    10141 atagatatct gtcagttcca gcaaagtaga agataacaac cgataacgca aatacggcca
    10201 ataagattcc caagtctgca gcgaacaagt tgaaagatct tcttctgtct gaggcggcac
    10261 aagtccagct attctttttc cttcttctaa cgcatggcga atggctgttg cactggctat
    10321 cctctgatct gtgatgattt tttcatgata gcctgcactt ttacgtcgga tcggatgtaa
    10381 ttccataggg tggacatact tggcattttc ctttgcataa cttaatccca agatatgatt
    10441 tggcgaagaa aaatcaagcg taatatcggg atacagactg cgaaaaacct cagtcatttt
    10501 ttgcggataa ctatacgttt gatcagacag ttcttgaaat gcctcttcta tcctatcttg
    10561 attctcttgg acaaagcgtc cgaaactgct gtaatcaaac gaagtctctt cttctgttcc
    10621 aaaacacaaa ctgtcacagt ccagtgtttg caagattttt actgcacctt tagcaaaata
    10681 gtcagctgac tgaaccgccc attcgacggg aagctccact actaaatcca caccattttg
    10741 taaagcttca ttcgcgcgat gccatttatc gatgatagcc ggttcgcctc tttgcaaaaa
    10801 attcccgctc attacagcaa tgacgacctc tgctcctgtt atttttcgtg ccatttccac
    10861 atgatagcga tgtccattgt gaaaaggatt gtactctacg ataatcccgc agcttttcat
    10921 gcgcatcctc cttcatagtt atcgataaaa aagaaataga gacaagtaaa aaaaggcaaa
    10981 aatcggaaaa aatacccgaa ctatataaac tattctcgca aacacaatac ttctgcgacg
    11041 aaaagctgca ctgcaagagc aatgcctata tcgttcggat atataattta tgttgaagat
    11101 ttattctttt cttgttaact cgagcaggca aaaccgctag tctttgtatg ctacaaagaa
    11161 ccagcgcttt gatttctctg atgctgcttc gtctgtaaaa tcagaaaatg cttcgacttt
    11221 cgaaaagccg ctgttttcca gcattcttaa ataactttcc aatggatagg tacgctcttg
    11281 atgtagttca tcttcacgaa taaatcgacc agacccttct tgttcttcaa cgaaaaaggt
    11341 aagaaaatgt tcgatgctgt gtttctcgct tcctggataa ctatcccaca aaaaagcaaa
    11401 attatcccct tgataatgat agctatattc tggaaaaacc tcatctactt gataggtgga
    11461 gtgtacgtca aataaaaatc ggccattttc ttctaacgct tgatagactt catcaaacac
    11521 ttgctgtacc tcttgtctat ttttcatata gcacagcgaa tcagaaaaac aggtaattgc
    11581 ctgatattgt ccgatttctg ataaatcgag catattgcct tccacaaatt ggatcgatgc
    11641 gtcttcttca tatgctcgct ggctggcaat catcaacatc tcttctgaaa ggtctaaccc
    11701 tgtcacttta tatccatctt ttgcaaaggc aaccgctaat gctccagtac cgcatgccag
    11761 ttccaaaata ttttttgtgt cttcttgtaa atgacgtttc gaaaaagcta accattgctg
    11821 ataaagtgtt tcgtccatca cttcatcgta aatgaaagca aatgtttcat aagccatatg
    11881 ttaatccaac cattcactaa gatcgactag cggagcatct gaccatagtt tttccagatt
    11941 atagaaattt cgttctggcg catggaaaac atgtacgatc acatctccaa gatcgatcaa
    12001 tacccatttt ccgccttctt ttccttcaat acgttttact tcatatttgt tttcttcttc
    12061 tttatcaatg atttcttctg taattgcgtt gatttgacgt tcgctatttg ctgaacagat
    12121 catgaaataa tctgccaaca aggaaacatt tcgaacgtct aaagcgataa tgtcttctgc
    12181 ccgttttgaa tctgctgctt caatcgcaat ttttaattgt tctttactat ctatgatgat
    12241 ttcctcctaa tatgagcagc ttattttttt gctacccagt aattatatgt ttcgattgtt
    12301 tttggataaa tctgttgttc ttgcgcaagc aaatgggcca atgtatgttt tgtttcaaat
    12361 gctacagctt cgtctagatc agcccatgca atggctcgtg catcttgtac cccaggaaaa
    12421 tctcgacctg gttcaatata gtctgctaca taaatgattt tgtcaaggag actcatctta
    12481 gcagccccag tcgtatgcac ccggattgca tgaagaatct ctgcatcggt gattcctaat
    12541 tcccgctcta caaacgaagc gccaacaaga ccatgccaga ttgcattatt ataattcaat
    12601 aactccggat caaaaccgtc tcttacgatt accattttga actcctcatc tggtcgttct
    12661 tttgcataat catgtgtcaa tgcagcaata ctagcttttt caggagaagc accgtatttt
    12721 ttggcaagag ccacggctgt ttcttctact ccaagcacat gcttgaagcg ttgttcgctc
    12781 attctcatct gtattttttg catcaggttt tctctagaat aagcagtata ttcattacta
    12841 tagtccatat tcatacaacc cttcattttg aatataatca attactttat ctggaacaag
    12901 gtagcggata gaacagcctt ctttgatctt ttgacgtatt ttcgtggaac tgatgtcgat
    12961 ctcaggtaca tctacccaaa ttacaggata tggagtatcc gttgtgtatc ctggtcgacg
    13021 tatacccaca aagttgacca tagaagtcag ctcatcgatt ttataccatt ttggcaaata
    13081 ttctaccata tccccaccga taataaagta ataatctgta tccggattgt tttgcgtcaa
    13141 ttccttcata gtatcatagg tgtaactttt cccaccacga gctaattcaa tcgtttcaat
    13201 ctgcaaaaaa ggattgtctt ccaccgctaa ttccaacatg ttgagtcgat gttttgcatc
    13261 aatcgtctgt ttctcatcaa catgaggtgg ctgataagaa ggcatcaaaa atacgcggtc
    13321 aagacctaaa ttccggccag cttgttcagc catcactaaa tgtgcaaggt gaacgggatt
    13381 gaatgttccg cccaagatcc ctacttgctt tttcttctct aagaattgag gcatttcttc
    13441 aggaaataat tgtgaacgaa caaatgtctt agcttgcgta ttcatcatgg tcactcctta
    13501 tattgctttt acttcttttg atagcctttg atacttttct tttgtggaag gtttgtataa
    13561 tacaataaca cgtccaatga tttggacgat ttcgcaatct atttcttttt ctaatacttg
    13621 agctgcttct tctgcaatct catctgtatt ttgaagtaac gatactttga tcagttctct
    13681 tttttctaaa gcttcttcaa tttgaacgat cattgctgaa ttgatcccac cttttccgat
    13741 ttgaaaaatc ggctgaagat gatgcgcttt gctacgtaaa aaacgctttt gttttcctct
    13801 taaactcata attttccttc ttccttaaat caatgctttg cgtttcaata catctacacc
    13861 ctttggcgcc cagcccgcaa ttactactgg cgctgttaca gtgatccagc ctagaccggc
    13921 aaaaacgatg tctgtttttt ctttcacaga aaactcaaaa cgaacaagct ccggaaatgc
    13981 ctcgacttca tcaggacgag gaggctgtaa taatccacct acatgttttt gataaaattc
    14041 gtctgctttt tccagtttcg ttcgatgaat atttacatca tttgacacat aagcaataaa
    14101 tgaagcacga ttgccgctga taaaatcaaa tcttgcaagt ccgcccaaga aaagtgtttg
    14161 cccttcattc agctgataaa ctttaggttt gatttctttt aacggtgcaa tgatccgcaa
    14221 atctttttta cctaagtagt gcgccatttg atggcgatgg ataattcccg gtgtatcaat
    14281 caaaaaatgc ccgtcatcta aaggaatctc gattttatcc aaggtcgttc ctggaaactg
    14341 cgaagttgtg attacatctt gtactcccgc tgtatttttg atgatttgat tgataagcgt
    14401 cgattttccg acatttgtta ctccgacgat atagacatcc cgtccatcgc ggtattcttc
    14461 gattttttct aatagttccg tcatctcatg agatttctta gcactagtta ataatacatc
    14521 tactggacga attcctgctt cgtaagcacg ttccttcatc cattgtgtta atttagtttt
    14581 tttcaatgat ttaggtaata tatccacttt gttcccaact aaaagtactg gattgtcacc
    14641 gacaaaacgg tgtagtcctg ggattagtga accattgaag tcaaaaatat ccaccacatt
    14701 gacgatcaat gcatcttttt gccctaattc attcagtaag cgcaaaaagt cgtcatccgt
    14761 caatgagaca tcttgggtct cattataatg gcgtaatctg aaacaacgct gacaatagac
    14821 ttccccggaa gctaaacctt tttccaaagc cgtttgcggc gtgtatccta attcaccggg
    14881 tttgtttgtt tgtataacgg caccacagcc gatacattgt aattcttctg tcattcgatt
    14941 ccacctcgcc agcccatatc agggtgtttt gctaatagat aggccataat tcttttttcc
    15001 caaaaacgat taaattgtgt cttccaacta tctgttgcga taatgggttt tacaaggatg
    15061 ctacgtatcc ctgcacgatt ggctccacgt atatcagtca taatctgatc gcctaccatt
    15121 actatctcat catctgctaa atccaattgt ttctttgcta tgttgatccc tcttgctaaa
    15181 ggtttcattg ctcgtgcaac atacaaaaga tcaaattttt caatcgctcg cgcgacacgt
    15241 tttgaattat tgtttgaaac aacaacaaca gggattcctg catttttcat ttctaaaatc
    15301 caagtaagca actcttctgt tccatctgga ttattccatg caatcaacgt attgtctaaa
    15361 tcggtcaata ctgctttgat gcctagattc tttaattggg caggtgtaat ctgatagatg
    15421 gcttctacca tccatgttgg tttgtaattt gaaaacatgc tgtctcctca tctgcaaaaa
    15481 atgaaggagc acataaagta ctcctcaacc ccgtattata ctgcatatca gcaaaaaaaa
    15541 ctacctctat actcgttcgt ctttcagaaa aagcatttct ttacaaaatg accaaaaata
    15601 aagattgatc acagaaaaat ttagaaacaa aaaacaacta atattggcgg aacctcttgt
    15661 atcgtttttc cagtaattta tgctgatcta atcgaacaag ttccgtcaat ctttcaatca
    15721 acgctttttt cataatgtgt gcgactttat atcgtggcaa ctgctcgccg ttgaacgttt
    15781 caggaataac tcgatcaacg attcctaact cgcgtagttc tggcgcagta attttcatca
    15841 actcagctgc ttctgcggcg cgactaccgt ctttccacaa aatcgacgca aatccttctg
    15901 gagaaagcac agcataaatc gtttcttcca gcatccagac ttcatccgct aaacctagag
    15961 caagggcacc gccacttcca ccttcaccaa tgatgatggc gatgatcggt acctttaaat
    16021 cagacatttc gagcagattt ctggcaatag cctctccttc gccgcgctct tctgctccta
    16081 caccgcaata tgcacctgct gtattgatca aggtaatcac cggtctattg aacttttccg
    16141 cttgtttcat cagtcgtaaa gcttttcgat agccttcagg atggggagaa ccaaaatttc
    16201 tgcggatatt ttcctgcaga tcccgtcctt tttgaatacc gatcactgtt accggtttgc
    16261 catctaatcg agcgattcca ccaacaatcg cttcgtcatc tgcaaataaa cgatcaccat
    16321 ggcattcctc gaaagaatcg aagatctctt caaaaaaatc caagcttgtc catcgttctt
    16381 tagctcttgc taactgaacg acatcatgtg ctgtcttatc cacgatctat ccatcctttc
    16441 tgtgtatgca attcaagaag cgtatggatt ttctgtttca attccatgcg gggaacaatc
    16501 tgatcgacaa aaccatgaga taataagaat tcagcctttt ggaaatcttc tggaagttcc
    16561 tgcttgatcg tctgttcgat gacgcgtctt ccagcaaaac cgatcaagct ttggggttct
    16621 gctagaataa tatcgccttc cattgcaaaa ctggcagtta ctcctccggt cgtcgggtcc
    16681 gttaaaacag ttagataaaa aagaccttca ttgctatgcc gtttgacagc agcagagatt
    16741 tttgccatct gcattagaga aaagattcct tcttgcatcc gtgcgccacc tgaagctgtg
    16801 aacaagacga ctggaagact ttcctttgtt gctcgttcga ataatctggt gatcttttct
    16861 ccgacaatcg ttcccatact tcccatgata aaattaggat ccatcactcc aataatgaag
    16921 ggaatcccct gaatggttgc tttaccagtg aggactgctt catgaagtcc tgttttatct
    16981 tgcatttttt taattttttc cgtatattcg ggaaatgcta gcggatcttt ggttacaaga
    17041 tccgtatccc attcttcaaa acttttttca tcaataatca aagctagacg ctgccatgca
    17101 ccgattcgaa aagcgtatcc gcaatgaggg catacttttt cttcaccgat gtctttcgta
    17161 taaaggatat gtttgcaatt tggacacttt gcccacatgt tatcaggaac agatggcgag
    17221 ttagcaggtt tgctctctct attaggattg atccgaatat atttcttttt cttaaacaga
    17281 gccatctatg cgcctcctat ctccgtttct ggtgtccaat tcggtaaaaa ttcttcttgt
    17341 aaaaatgctg tgctgtaatc acctgctatg actgcaggat gactaatcaa atccatctga
    17401 aattctgcgt tcgtgatgat tccttctgtg ataaattcac ttaaggctcg ctgcatcttc
    17461 attaatgctt caaaacgcgt atttccatgg acgattattt ttgcaatcat tgaatcataa
    17521 tatggcggga tcgtatatcc cgggtaaact gcactgtcta ctcgcagtcc catcccacct
    17581 gctggaagca gtaagttttg gatcttccct ggagaaggag caaaatggaa agcaggattt
    17641 tctgcattaa tccggcattc gattgcgtgt ccttggaata ctacttcttc ttgacacaca
    17701 ttcaaaggct caccggctgc tatctccacc tgttttttta ctaaatcgat tcctgtgacc
    17761 atttctgtta ctggatgttc cacttggatt cgagtattca tttccatgaa ataaaaatcg
    17821 ccttcttcat ccatcagaaa ctcgatcgtt ccggcatttt cataattgac tgctttagct
    17881 gctcgaacag cagcgtctcc tagtgcttct ctttttgttt gtgagatcac tacagaagga
    17941 gattcttcca gtactttttg attgttgcgt tgtaaagaac aatctcgctc gcctaaatga
    18001 atgacatgac caaatcgatc acctaagatc tgaacttcta tatggcgagc aggataaatg
    18061 attttttcca aatacatatc atcatttcca aatgccgctg ctgcttcttg ctgagctgaa
    18121 gaaaaatgct gtgggagctc ttcttttgat tgaactttcc gaatgccttt cccgccacca
    18181 cctgcagctg ctttcagcat cactggataa ccaattctgt ctgctatttc caatgcttct
    18241 tctactgtag ataatacacc agtacttcct ggaatgactg ggacatttgc ttcttgcatc
    18301 aattgacgcg cattgatctt atttcccatt gcatcgatcg ttgcactttt aggaccaata
    18361 aatgtgatat tgcactcttc acacatggag gcaaatcggc tattttcaga taaaaaacca
    18421 aatcctggat gaattgcttc ggcttttgtc acgattgcag cgctcagtac ttcctgaaca
    18481 ttcagatatg aatcaacggc ttttgccggg ccaatgcaga ttgcttcatc tgctaactgt
    18541 gtatgaagtg cttcggcatc tgcttctgaa tatatcgcta cggtttggat ccctagttcg
    18601 cggcatgcac gaatgatacg tacagcgatt tcaccgcggt tagctatcaa tactttagaa
    18661 aacatatttc acctatccaa tcataaaggt taattccgct tcggctactt ttttaccgtc
    18721 tactttagcg attcctttgc ctattccggc tgaagctttc actttcaata tctcaacttc
    18781 aaggtataag gtatctcccg gtgtaacttt cttacggaat tttgctttgt ccaatccgcc
    18841 gaaataagcc gtctttccct tgaattgttc aagagaaagt aatgcaactg ctcctgcttg
    18901 agccattgct tcaacaatca atacccctgg cataacaggt tcttctggaa aatgtccttg
    18961 aaaaaagggc tcattgatcg ttacattttt cttagcaacg atccgttctc cttcaaccat
    19021 ttcttccact cgatcaatca aaagcattgg ataacgatgt ggaatgattt cttttatttc
    19081 ttgtatgttc attgttttcc ctctttcctg ctttgcttat ttttgctgaa tcctaaagag
    19141 tggctgtcca tattcgacaa ctgactcgtt ttctacaagg atttctgtga tcactccgtc
    19201 ggcctcacta gttatctcat tcatcagttt catcgcttca ataatgcaga taacttctcc
    19261 tttggcaact tgatcaccta cttttttgaa agctggtttg tcgggcgctg gttgcagata
    19321 aacgattccc acgattggcg aaacaacctc gattccatca aatactgccg attctttttc
    19381 tttcattatt tctggagcag ttgttgcttg ttcaggtatg acattgactg gctccgaaac
    19441 tggcaacgtt tcgtttgttt gaaccatatt catgcctcga cttgttttat ttttattcat
    19501 atacaattca aattgacctt cacgcaaatc gaattccgtt agcgaggatt gatcaaattg
    19561 actgactaat tctctgattt cattgatatc caaaattatt cctcccagcg tttcatgcac
    19621 agaaccgcat tatgtccgcc aaaccctaat gaattactca ttgcataagt aaaacttgca
    19681 ggtttgcttt cgtttttgac aactgtcaag tcaatctcag gatcttgttt ttctacattg
    19741 atcgtaggtg ggataaattg atgttctaat gccagcaaag ttgccaaggc ttcgatcccg
    19801 ccggctgctc ccaataaatg tccagtcatc gatttcgtgc tgctgacgta agtatctctg
    19861 aaattttcac ctaatgcgat ttggattgct tttgcttctg cctggtcatt ggctggtgta
    19921 cttgttccgt gagcattgat atatccgact tgttccggct gtacaccagc ttcttccatc
    19981 gcaagcttca ttgctttaga tgcaccagaa ccatctggag taggagctgt catatggtaa
    20041 gcgtcacagt ttgcaccgta tccaacgatt tctccataga ttttggcacc gcgagcttgt
    20101 gcatgttcta aagattcaag aactagaacg cctgctcctt cacccatcac aaaaccattc
    20161 cgattttcgt caaacggaat cgaaccacga ttaggatcag ttgattctga gagtgctgtt
    20221 aaagctgcaa atccagcaat ccccatctca caaatcgttg cttctgttcc tccagcaaga
    20281 atgacatcag aatagccatg tttgatgtta cggaatgctt ccccaattga attcgttccg
    20341 ctagcacaag ctgttacgat agctgtacaa ataccttttg cacctacacg caaagcaata
    20401 tttccagctg ccatgttggc aatcgccatt ggtacgaaca ttggagaaac tcgttttgca
    20461 cctttttcat gtagacgaat aacttggttt tcgattgttg gaagaccacc gatcccagat
    20521 ccgatgatga cgccaaaccg gtcttgattc attttttctt tatctaaacc gctcatctcc
    20581 attgcttcta aagcagcgta gattccatag atcgaaaaca tatccattcg tttgctatct
    20641 tttttaataa aatatttatc gtaagggaaa tctttgactt cacccgctaa ggtaatccct
    20701 gtttctgaag catcaaattt agtgatcggt gcgatgccat tcgtcccctc ttttaagcta
    20761 ttcaaaaagc tgtctgcgtc atttccaata ggtgaagtga ctccatagcc agtaattacc
    20821 actcgattca tttgtttgct ctccttttct tatccgtgca tgaccaatcc gccatcgaca
    20881 ttgatgactt gtccggtgat atatggattt tgtgctaaga aaactgctgt ttgtgcaata
    20941 tcttctactt gtccgaaacg ctgcatcgga atttgctttt ccgcttgttc cttgactttg
    21001 tctgctaaaa cttccgtcat atcagttgtg atgaatcctg gcgcaatggc attacatgta
    21061 atgcctctag ttgcagcttc tcgtgccaca gattttgtta atcctactac gccagcttta
    21121 ctagctgcat aatttgcttg accaatattt ccaatcaacc cagatacact agagagatta
    21181 atgatggccc cttctcgctg cttcatcatt tttttcaata cgtgctgcgt catattgaag
    21241 gttccaatca aattgatatc cagacatttt ttgaaatctt ctgcatccat tcgcatcaca
    21301 agtttgtcat ttgtgattcc ggcattgttt actaacacat ggatcgatcc aagcttttct
    21361 tctgcttctt tgatcatttg tcccgctttc tcataatcag agatgtcacc ggaaactcct
    21421 acgcatttta caccgaatgc ttcgatttct tctatctttt cttttggaat ctctccgcga
    21481 ccattcaaaa tgatatttgc tccagctttt gcaaaagcta aggccatcgc tttgccgatt
    21541 cctcttgtac tacctgtgat aaatacattt tttcctttta catccataac gttcctcctt
    21601 atagtattgc tattgtttca tcaaatgtct gcttattctc aacacgcaga actggtattg
    21661 ttttatcgat ttttttcata aatccgctta atacttttcc ggggccgact tcgatgactt
    21721 gttcgatgcc gattgttttt aacttatgga tactctcata gaaacgaaca ggttgcatga
    21781 cttgctgttc aagtagtccc gcgattgttt ctttcttcat tatttccgtt gttgtgttgc
    21841 ttatgatagg aaaagaaggt tcagagaatt gaatctgact taggtccttc gccagttttt
    21901 ttgctgccgg ttctaaaata gctgtatgaa aaggcccgct gacatttaga gggatcattc
    21961 ttttgaaccc tttttctttt aatagtaaaa cagcttcgtc tactgctttt tcttcgccac
    22021 caatcacgat ctgctgaggt gtattataat ttgcaggaga tacgatgccg tacttgctag
    22081 cttcatgaca gctttcctcg atcgtttcaa tcggcgcatt catcactgca accattttcc
    22141 cgcttccagc tggggcagct tctgtcatat aagcgcctct ttttgctact aacgcaactg
    22201 cttcagtgaa ggatagcgca ttactagcta ctaatgccga atattctcct aaactcagtc
    22261 ctaaagctgc atctgggatg attccatgct ccttcaataa gcgataatac gcgatgctga
    22321 ctgttagaat agctggctgt gtatattgcg tctgatccaa acgttcattt tctgtaaagc
    22381 aaagttcagc catatcgtag ccgagaatct cacttgcttc atcaaatgtc tgctttacaa
    22441 cggcttcttc atataaatct tttcccatac cttgatattg tgcaccttgg ccactaaata
    22501 agaatgctgt tttcatgttt ttctccaata tatccagaat atatgtttga ttcttcctat
    22561 tcaaaaaggc cgagacattc ctatttcagg aaatgtccca gcccccaacc taattttaat
    22621 gattatgcag tttgtttttc tacgtaagtc actaaatctt gaaccgtttt gattccgtct
    22681 tctgtttcga ttttcacgtc aaattcatct tcaatttcat tgatgatttg gaataaatcc
    22741 aagctgtcag cttctaagtc ttcttggatg tcagtagtca atttgatttc ttcagtttct
    22801 tttcctaatt cttctgcgat gatttcttga attttttcga ataccataat aatatattcc
    22861 tccgatattg tttgcttttt atttttataa tttaacagtc tacaactcca tgagaatact
    22921 tccccatgtt aaacctccgc catagcctgt aaatattact ttctgctgtt ttcctaaggt
    22981 gatctttcca ctagaaactg cttcatccaa taagataggg atgcttgcag cagatgtatt
    23041 cccatattta tccatattgg tcaggaactt ttcttgcggc atctttactt ttcgggcaat
    23101 tttctcaata attctgaggt ttgcctgatg caaaagcaga taatcaactg gtttgtcttt
    23161 catgacttct tggatattgt ttgcaacgtc tcgtacggca aaatcaaaaa tatctctgcc
    23221 agtcatttgc aaataatacg aaccttctga atcttctgaa tagaacggat ttttattttg
    23281 ctggtagcca gcggtcaatg agctacttct ggtaccgtca gctgctaatt tttctttcag
    23341 caattttttc tgtggattcg cttctaagag aatcccacct gctccatcgc caaacaacac
    23401 agcagtcgta cgatcagacc aatcgattag cttagatagt gtttcaccac caatcaccag
    23461 tcccttagtt gagccgcagc ggatcatttt ttcagccatg cttaacgcat aaacaaatcc
    23521 tgagcacgca gcactgatat caaaagcgat agcatgagtt gctcccaatt tcccttgtac
    23581 ttgtgctgct acagatggtg tcgtgtaatc gggcgacatt gttgctacta aaataaaatc
    23641 caaatcttta gggtttagat ttttctgttt tatcaatttt tccgccacta aaatgcataa
    23701 atcagaagtc tcctgatttg ttacacatct tcgttcacga atacccgtcc ttgacgaaat
    23761 ccattcatca gaggtatcca tcatcttgga aagatcatta ttagtaataa tcttttcagg
    23821 aaggacgctg gctgttgcag taatcattcc aaattgttcc attaaatatc ctcacttata
    23881 gtcttgcaag aagtcgtgca agtttttcag tgcatccagc aaggcatgct gttcctccgt
    23941 agccattccg tctaagatgt tcttgaccat ttcacgatga aaatgctgat ggacccgaaa
    24001 tagtaacttt ccttttttcg ttagtcccaa ctttacaacg cggcggtcat cttcactgcg
    24061 gatacgttcg acatagcctt tttttactaa cttgttaata gcagtggtca aggtcccaac
    24121 agtaattgat agttctttgg ctacttctga agttgttttt ttcttgtaca tcccgattgc
    24181 ctcgatcgtg tgcatctctg tgattgacag atctttgaat tgagattttt tcagctcaga
    24241 ctcttcgatt gtcaaaatat cattgaagac actaacaaga taatcattta ctgtttttaa
    24301 atctggttcc atgttccacc gtccactaat tagtttgatt atcaaatctt ttgatgttca
    24361 aactatatct taactttttt caaaatgcaa ccttaaaaga tcattttttt actatatgag
    24421 ctttttttaa gattttactc atttaaaaca actgataaat cagcatttat aatt
//
LOCUS       DAZULO010000032        24435 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000032 DAZULO010000000
VERSION     DAZULO010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.033"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..788)
                     /locus_tag="ACG8OA_001307"
     CDS             complement(54..788)
                     /locus_tag="ACG8OA_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS4976105.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="ACG8OA_001308"
     CDS             1115..1525
                     /locus_tag="ACG8OA_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFS4976106.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="ACG8OA_001309"
     CDS             1518..2219
                     /locus_tag="ACG8OA_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFS4976107.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="ACG8OA_001310"
     CDS             2219..3805
                     /locus_tag="ACG8OA_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4976108.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="ACG8OA_001311"
     CDS             3824..4045
                     /locus_tag="ACG8OA_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFS4976109.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="ACG8OA_001312"
     CDS             4042..4803
                     /locus_tag="ACG8OA_001312"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFS4976110.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="ACG8OA_001313"
     CDS             5183..5692
                     /locus_tag="ACG8OA_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS4976111.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="ACG8OA_001314"
     CDS             complement(5767..6306)
                     /locus_tag="ACG8OA_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFS4976112.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACG8OA_001315"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACG8OA_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFS4976113.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="ACG8OA_001316"
     CDS             7319..8170
                     /locus_tag="ACG8OA_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFS4976114.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="ACG8OA_001317"
     CDS             8198..8833
                     /locus_tag="ACG8OA_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFS4976115.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="ACG8OA_001318"
     CDS             8853..9626
                     /locus_tag="ACG8OA_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4976116.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="ACG8OA_001319"
     CDS             10034..10831
                     /locus_tag="ACG8OA_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFS4976117.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="ACG8OA_001320"
     CDS             10809..11222
                     /locus_tag="ACG8OA_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS4976118.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="ACG8OA_001321"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="ACG8OA_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS4976119.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="ACG8OA_001322"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="ACG8OA_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFS4976120.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /locus_tag="ACG8OA_001323"
     CDS             15999..16559
                     /locus_tag="ACG8OA_001323"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFS4976121.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACG8OA_001324"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACG8OA_001324"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFS4976122.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="ACG8OA_001325"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="ACG8OA_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFS4976123.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="ACG8OA_001326"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="ACG8OA_001326"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFS4976124.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="ACG8OA_001327"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="ACG8OA_001327"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFS4976125.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="ACG8OA_001328"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="ACG8OA_001328"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFS4976126.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="ACG8OA_001329"
     CDS             21906..22484
                     /locus_tag="ACG8OA_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4976127.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="ACG8OA_001330"
     CDS             22536..23018
                     /locus_tag="ACG8OA_001330"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFS4976128.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="ACG8OA_001331"
     CDS             23135..23509
                     /locus_tag="ACG8OA_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976129.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            23939..>24435
                     /locus_tag="ACG8OA_001332"
     CDS             23939..>24435
                     /locus_tag="ACG8OA_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFS4976130.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT         8011 a         4391 c         5213 g         6820 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgag
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcacccggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatagaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaata atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc attagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaagtttt tgagagtata aaatattttg tgtaaatgaa aaaatccata caaaaaagga
    23881 agtcccttct gtagaataaa gttaacgaca accaattcac agaaaagagg acttccctat
    23941 gaatgatttt actacagaaa ttgtgcaaac tctagtcact aaaggcgatt taaatgaatt
    24001 attccgttcg cacttagaaa aagcgataaa cacactccta cggactgaat taacggcttt
    24061 tttagattac gaaaaatatg atcgcactgg ttttaattca ggtaattcga gaaacggttc
    24121 ttactttcga tcaatcaaaa ccgaatatgg tgaattaaca ttggaaatac ctagagatcg
    24181 taatggtgag tttaaacaac aaactttacc agcctacaaa agaacaaacg atacattgga
    24241 aaccactatt atccatttat tcgaaaaagg tgttacgatg tctgaaattg ctgatttgat
    24301 cgaaaaaatg tacggtcatc actatactcc acaaaccatg tccaacatga ctaaagttct
    24361 gactgaagaa gtaaatgcct ttaaatccag agccttaaat gataagtatg tcgctatttt
    24421 tatggacgct actta
//
LOCUS       DAZULO010000033        23416 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000033 DAZULO010000000
VERSION     DAZULO010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.034"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..1690
                     /locus_tag="ACG8OA_001333"
     CDS             29..1690
                     /locus_tag="ACG8OA_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4976131.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1760..2107)
                     /locus_tag="ACG8OA_001334"
     CDS             complement(1760..2107)
                     /locus_tag="ACG8OA_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFS4976132.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2191..3132
                     /gene="opp3b"
                     /locus_tag="ACG8OA_001335"
     CDS             2191..3132
                     /gene="opp3b"
                     /locus_tag="ACG8OA_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS4976133.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3133..4176
                     /locus_tag="ACG8OA_001336"
     CDS             3133..4176
                     /locus_tag="ACG8OA_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4976134.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4182..5252
                     /locus_tag="ACG8OA_001337"
     CDS             4182..5252
                     /locus_tag="ACG8OA_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4976135.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5242..6195
                     /locus_tag="ACG8OA_001338"
     CDS             5242..6195
                     /locus_tag="ACG8OA_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4976136.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6207..7217
                     /locus_tag="ACG8OA_001339"
     CDS             6207..7217
                     /locus_tag="ACG8OA_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFS4976137.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7502..8323)
                     /locus_tag="ACG8OA_001340"
     CDS             complement(7502..8323)
                     /locus_tag="ACG8OA_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS4976138.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8420..8698)
                     /locus_tag="ACG8OA_001341"
     CDS             complement(8420..8698)
                     /locus_tag="ACG8OA_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976139.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8966..10975
                     /gene="metG"
                     /locus_tag="ACG8OA_001342"
     CDS             8966..10975
                     /gene="metG"
                     /locus_tag="ACG8OA_001342"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFS4976140.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11454..12041)
                     /locus_tag="ACG8OA_001343"
     CDS             complement(11454..12041)
                     /locus_tag="ACG8OA_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976141.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12216..13538
                     /locus_tag="ACG8OA_001344"
     CDS             12216..13538
                     /locus_tag="ACG8OA_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4976142.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13693..14469
                     /locus_tag="ACG8OA_001345"
     CDS             13693..14469
                     /locus_tag="ACG8OA_001345"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS4976143.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14462..15031
                     /gene="rnmV"
                     /locus_tag="ACG8OA_001346"
     CDS             14462..15031
                     /gene="rnmV"
                     /locus_tag="ACG8OA_001346"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFS4976144.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15024..15908
                     /gene="rsmA"
                     /locus_tag="ACG8OA_001347"
     CDS             15024..15908
                     /gene="rsmA"
                     /locus_tag="ACG8OA_001347"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFS4976145.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16359..16988
                     /locus_tag="ACG8OA_001348"
     CDS             16359..16988
                     /locus_tag="ACG8OA_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976146.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17112..18221)
                     /locus_tag="ACG8OA_001349"
     CDS             complement(17112..18221)
                     /locus_tag="ACG8OA_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4976147.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18430..18852
                     /gene="mgsA"
                     /locus_tag="ACG8OA_001350"
     CDS             18430..18852
                     /gene="mgsA"
                     /locus_tag="ACG8OA_001350"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFS4976148.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19039..19137
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19175..19711
                     /locus_tag="ACG8OA_001351"
     CDS             19175..19711
                     /locus_tag="ACG8OA_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS4976149.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19751..21514)
                     /locus_tag="ACG8OA_001352"
     CDS             complement(19751..21514)
                     /locus_tag="ACG8OA_001352"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4976150.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21517..23232)
                     /locus_tag="ACG8OA_001353"
     CDS             complement(21517..23232)
                     /locus_tag="ACG8OA_001353"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4976151.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7541 a         4290 c         4773 g         6812 t
ORIGIN      
        1 tgaaaattcc aataaagggg tacgtaaaat gaaaaagaaa tttacatttg gtttggttgc
       61 ggtatgcggc cttgtacttg ctggatgtta tggcggcagt tcagccacaa gcagtagttc
      121 agcaaaaagc ggttcagcag atggaggagg cgtatttaac ttagtcgtgc ctcaagaaat
      181 gcctacagct gacttatcag ttgcaacaga tacaatcagt tttacagcat tgaataacgt
      241 ttatgaagga atttaccgtc ttgatgaaga cagcaaacca cagccggcag gagctagcga
      301 gttggcagaa gtcagtgaag acggattaac ttacaaaatc aaattacgag aagacgcaaa
      361 atggtcaaat ggtgatcctg ttacagcagc ggattacgtt tatggctggc aacgaacagt
      421 agacccagct acagcttctg aatatgctta cctgtatgcg ccagttgaaa atgcagaaga
      481 cattacagct ggaaagaaag ataaatcaga actagggatc aaagcagttg gtgactatga
      541 attagaaatc aaattgacga aacaaacacc atatttccaa tatctgcttg ctttcccatc
      601 atttttccca caaaaacaat ccgtagtgga agaaaatggg gatgcctatg catctgcaag
      661 tgataaagct gtttataatg gaccattcac attagaagat tttgatggac caggaacaga
      721 tacagaatgg acttataaga aaaatgatca ttactgggac aaagatacag taaaactttc
      781 tgaaatcaaa gtcagtgtag taaaagaatc ttctactgca ttgaacttat tcaaagatgg
      841 ccaagcagat gacgtgatct tgtctggtga gttagcacag caaaacgcaa acgatccagc
      901 ttatacttct gttaaagaag cgcggacaag ctatatcgaa ttcaatcaac gtgaaaagaa
      961 ttcaccattc aacaatgtga acctaagaaa agctatttct tattctatcg atcgtgaagc
     1021 tttagtaaaa caagtactag gtgacggatc agttgtctct acaggattga tcccatcaga
     1081 tatggtaaaa agtccaacaa cgaacgaaga ctttgcaaaa gaagctggaa aattagtttc
     1141 ttatgacaaa gataaagcaa aagaatattg ggaaaaagcg aaaaaagaat tgggcgttga
     1201 ttctttagaa tttgatttga tggcttctga tgatgattca tcgaaaaaag taatcgaata
     1261 tgttcaaaac tcaatccaag aaaacttgga tggcgtaaca gtcaaaccaa cacctgttcc
     1321 attctctgta cgtcttgacc gctcaacttc gggtgatttt gacgcagtac taggcggatg
     1381 ggctgctgac tatgcagacc caagcagctt tactgatttg tttgtcacag gtaattcata
     1441 caaccgtggt cgctggagca atgcagaata tgacgaagca gttaaagcag cttcatcaac
     1501 agacgctggc gatgaacaag cccgttggga tgatttccaa aaagcaatca agatcatctc
     1561 tgatgatatg ggtgtcgttc cggtgtacca aaaagcagaa ggtcacctag tgaatgaaaa
     1621 aatcaaaggt atcgttcacc atgcggcagg cgcttcatgg gattacaaat ggacatacgt
     1681 tgaagaataa tcgttaatac cgtgagcatg agaaaaaacg aggctgcggc aaatgtcgca
     1741 acctcgttct catattcact tatcctaaca aagaaaaaag catgaaaata gcagaaagag
     1801 caattagtat tccggcaatg atcagccaaa agctgtacat accgtagctg gcagacgaga
     1861 gcgaggaaaa ttctggtttt ttctttcggt tccttgttcg tgcaagatgg accgatcgct
     1921 gaaaatgatc gaagaaaccc gaagaaaaga cccataaaaa accgccgata atcagaaacg
     1981 gcaatgacca taagaataga tcattagata attgaatgag tgtaaaggaa tggttgatga
     2041 gattttttat taaaataacc agaatcagta gaaaagctac tgcgtagggg atagattttt
     2101 ttttcatttg attgctcctt tatttttggt tcattatcta ataatgtagc gaaacatctg
     2161 tcagaagtca aaagctaggg ggaaacatta gtgaatagtt atatcaaata tgtattaaaa
     2221 cgtgtctttt tcatgatcat cacgctttgg ttaattgcaa caatcacctt tttcttgatg
     2281 caattgctgc cgggcacgcc atatacgaat caggaacgtt tgagtcctga aacaatcgag
     2341 atgctaaata aacaagtcgg tttggataaa ccagttatcg tacaatatgg gatttatctt
     2401 tctaatttgc ttcaaggaga ttttggaatt tccttccaat ttaaaaacca gccagtcgca
     2461 catttgttag ctggaagaat cggaccatcc ttgcagttag ggttacaggc aattatcttt
     2521 ggaacagtcc tagggacgat tttaggaacg atctctgcca tgaagcaaaa tacatgggca
     2581 gatacatctt ctacactcgt agcgatatta ggtcgttcca ttccaaactt tgtatttgct
     2641 gttttattac aatatatctt tgcaatcaaa ttacaagttt tgccaatcgc aaaatgggat
     2701 ggctttgtgt acacaatctt accaacaatt gcgttggcca tgtcgccgct tgctgactct
     2761 gctcggttta tccgtacgga aatggtggaa gtactgcata gtgattatgt ggaattagca
     2821 aaagcaaaag gattgagccg ttgggaaatc gcctttaaac atggtcttcg aaatagtttg
     2881 attccattga tgactttatt aggaccgtta gctgtagcat tgatgaccgg atcactggtg
     2941 gtagaaaata tctttgctat tcctggtatc ggggaacaat tcgttaagtc aatcacaaca
     3001 aatgattatc ctacgatcat ggctgtaacg atcctttact catttatgct gatttttgtt
     3061 attttgatcg tggatctatt gtacggtcta gttgatccaa gaattcgtgt ttcagaaggg
     3121 agccgaggat aaatggaaat gataccaaag cgttatgaaa cagttgcaga tatccctgcg
     3181 gatgaatttc ttccattaca aaaaaatacc gaagaagaac gtgaacagat tactgcacct
     3241 tctttgaatt ttatccaaga ttcatggcgc cgtttaaaga aaaacaaagc tgccgttatt
     3301 tcaatggttt tactattggt catcattttg atctctgtga tcacaatctt tgtttctcct
     3361 catgatccaa ctgcccaaaa cgtggcttat atcaatttgc ctcctcgtat tccgggaatc
     3421 aatatcgatg gattgaatgg aaaagcaatg gttggtggtg aacttgttga taaatatgct
     3481 caagcaaacg tcccttcaaa cgtaaatttc tttttaggaa cagacggttt gggacgtgat
     3541 gtattaagcc gcttatttat gggaatgaga atctcgttgc tgatcgcatt tattgctgca
     3601 ttactagatg taacgattgg tgtagcttac gggttggttt ctggtttgtt aggcggacgt
     3661 gtcgataacg tgatgcaacg ttttttggaa atcttatcag gaattccaaa cctcgttgtc
     3721 atgattttga tgttggtcgt ttttgaacca ggaatttttt caatcgtggc tgctatggcc
     3781 atcacaaact ggataccaat ggcacgtatc gttcgtgcac aaacaatgaa attaaaagat
     3841 caagaatatg ttttagctgg attgacatta ggtgaatcaa gaatgaaaat tgcattcaaa
     3901 catattttgc cgaatatctc tagtgtcatt attattcaaa tgatgtttag tattccatct
     3961 gctattttct tcgaagcctt tttaagcttt atcggtttag gtcttacgcc tccgtcagct
     4021 tcattaggaa cgatgttgag cgaagggtac aagacattcc tgtatctgcc ttatctatta
     4081 tggattccgg cagtaacttt atcggttatc atgattggct ttaacttact tgctgatgga
     4141 ttgcgtgatg cctttgatcc taaaatgaaa gagtgaagac gatgacgaaa attttagaag
     4201 tgaaagactt agaaatctct tttgatacgt atgctggaaa ggtacgagcg attcgcggtg
     4261 tcaattttca tttgaataaa ggagaaacat tggcgatcgt tggggaatct ggaagtggaa
     4321 aatcagtgac gaccagaagc atcatgcgct tgctgtcaag caacgccaat atagatgctg
     4381 gtcaaatcct ctttaaaggt aacgatatcg ttggaaaatc agaaagagag atgcaaaaaa
     4441 tccgtggtaa agaaatagcg atgattttcc aagatccaat gacctcttta gatccgacga
     4501 tgcccattgg gaagcaagtc gctgagtccc ttagaaagca taataaagtt tcaaaaaaag
     4561 agggacaaaa agctgcatta gaattgctta aattagttgg aatacccgaa gctgagaaac
     4621 gtatcaacag ttatccgcat cagttctcag gaggtcagcg tcaacggatc gtgatcgcga
     4681 ttgcactggt atgttatccg gaaatcctga ttgctgatga acctacaaca gcgcttgatg
     4741 tgactattca agcacagatt ttggagctgc tgaaagaaat tcagacaaaa atcgatacat
     4801 caattatctt tatcactcat gaccttggtg tagtagcgaa tgtcgctgat cgtgtcgctg
     4861 tcatgtatgg aggaagaatc gttgaagttg gaacatcgga agaaattttc tacaacccgc
     4921 aacatcccta tacttgggga ctgcttggtt caatgccgac attagatagt gcgaacgatc
     4981 gattgtatgc tattcctggt tctccaccgg atttattgaa cccgccaaaa ggagacgcat
     5041 tctatccaag aaatgaattc gcgatgaaaa tcgatgcaga acaagaacca ccattttttg
     5101 aactatcaaa aacacacaag gcagcaactt ggctacttgc tccgcaggca cccaaagtga
     5161 caccgccaga agaaatccaa cgtcgctggg ctatctttaa agagaaacaa aaacatactt
     5221 acggtcaagg aggaattgct gatgccaaat aaactacttg aagttaaagg attgaaacag
     5281 tatttcaatg taggaaaaaa gaatgaagta catgctgtaa atgacatcag ctttcacatt
     5341 tatgaaggag aaacattcgg tttagttgga gaatctggaa gcggaaaatc cactactggc
     5401 cgtacgatca ttcgcttgaa tgaacctact gatggtgaga tattattcga cggtcaagat
     5461 ataacgaaga tcaaagataa aaaaggtttg acgaaattcc ggcatgatgt tcagatgatc
     5521 tttcaagatc catacgcttc gttgaatcct cgtatgaaag tcagagatat cattgccgaa
     5581 ggaattgatg ttaatggact agctaaatca tctgaagagc gtgcaaaaaa agtcgatgat
     5641 ctattgagaa ctgttggatt gaatccaagc cacggtactc gttatcctca tgaattttca
     5701 ggtgggcaac gccagcgtat tggtattgca cgagcattgg cagtaaaacc gcgttttatc
     5761 atttgtgatg aaccaatttc tgcactagat gtgtcgatcc aagcccaagt ggtcaatttg
     5821 ttgcaagatc tccaaaaaga acatcaattg acctatctat ttattgcaca tgatttgtcg
     5881 atggttaaac atattagtga ccgaatcggt gtaatgcata acggcttgtt attagagatg
     5941 ggaacaagtg atgaaatcta taatcacggt gttcatcctt atacagaaag tttgttatct
     6001 gctattcctc taccagatcc agaccatgag agaaaacgca gaagaatcaa atatcaacct
     6061 gaaccagatg acggacaagt acgtcaactt cgtgaaatcg caccagaaca ttttgtctat
     6121 gcaactgagc aggaagtccc atattacgca aaaaaactaa aacgacaaaa ggaatctctt
     6181 ctggttgcta actaggaggg acatagatgc taaaagcttt taaattccgt atgtatccga
     6241 cagaagaaca aaaacagcaa ttgattcgta cttttggatg tgctcgattt acctataacc
     6301 atttgctgaa aaagcggcag aaaagttggc aacaaacagg agtagccaat ttttcgctca
     6361 cacccgctac attaaaaaaa gaatacccat tcttaaaaga agtagacagt cttgctttag
     6421 caaatgcaca gcttaatttg gatcgggctt ttcgcaatta tttcaaggga cgtgccagct
     6481 tcccaaagct taaaacgaaa aaaagtatgt ggcaatctta tacgaccaac aaccaaaccc
     6541 gtacgatcta tctggaaaat ggctacttaa agttaccaaa acaaaaagaa ctaatcaaga
     6601 taaatcagca tcggccagtt gaaggatcga ttcgctccgc tacgatttct gctcgatata
     6661 atgaagaatt ttatgtcgca ctactttgtg atgtttcgcc agtaaaaaaa gaatctttag
     6721 ctaaatggat cgggatcgcc tatcatccga agacattgat ccaaacatct cgacctctag
     6781 aagtaacttt accaaaattt caccagacag aagaaaagct gcaacatgca cagcggaaat
     6841 tgaatgtgaa agtccgctcc gctcaccata gaaagatcag gttagatcag gcaagcaatt
     6901 atcagaaaca aaagagaaaa gtaatggatc tttatctgaa acaaaagaac cagcgagaag
     6961 attatcttga acaattatct gggaaattgg tcaaacaata tgactactta tttgtagaat
     7021 cttttccaaa agaagaggca catgctgatt tttctattca cgactggcat aaattaataa
     7081 caaaattgcg ttataagtcc caatggtata ataaaaaatt tcttttgatc aataccgacg
     7141 gagcagaaga aagcaattcc gtaagaaaga gccaagtagt agaaaaaatg ggcagacatt
     7201 cggttatcaa gggataaaaa atgaatgaaa ctgataggag cgttagggtt agccttgtta
     7261 ataaaaagtc ttaatgactt ttgtttctag gaatttttct tttgaagaaa agctcactaa
     7321 ttatgacaaa ccaataaaac aatagcagaa aaagttcgta cgagcttttt ctgctattgt
     7381 taatttttta ttcagaaagt ttacgaatac atcaaaattc tgaaacaaaa aacgtaactc
     7441 acaaagagtt gtttttgcgt tttttcttga atgattaggt ctgaatcttt tgctcacaac
     7501 cttattcatg gtgccccggc ttgagctgat agatttttga aggacggccg attcctacag
     7561 gacgttcacc gatctcatcg aaatattgga gcatagcttt cttaaaattg gaatgatcga
     7621 tcgttttata atcgatccct aagaatttag caaatacttt tcgagcttcc gtaatcgtga
     7681 aatctttacc taaaacttgt agaacttgcg gctcgtgttc cattttattg actacacggt
     7741 tgaatgcttt caagatgatt tgactatggt caaaagccaa agtatctttg ccagaagact
     7801 ctccagtgac taaatcaagc gagatagcca cttctccgct agataaatgg atcttgttac
     7861 tatgacgttc tagtgtgaac cagcgtactt ccttggcatc atctccggcg attagcggtt
     7921 cttctccaat aaaggctaag tagctgactg tgacgaccca gcctcgcgga tctcgattag
     7981 gcgtactgaa tgtatgcagt tgctcaatat tttcttttga aataaccaca ccagtttctt
     8041 cctttgtttc tcttaagacg ctttctccag ttgactcatc cttttggaca aagccaccag
     8101 gcaatgccca tgaatttcga taaggatgcc cttttctttg gatcaacaaa actttcagct
     8161 gatcttcttc tttgttatag cacatcagta cgatatcgac agtcaaagag ggcttgtcat
     8221 attctggacg ttcctgtttt tgataccagt taagaaattc ttcttcactt gctttatgtt
     8281 cataatataa tttttcttct tctttagaag caaaacggtt catctgacca cctccattga
     8341 cagtaaaagt actttttacc ttattgtagc agactctgaa aaaaaaccaa ggatttactt
     8401 gaaaaatcta ggttatcatt taaatatgaa aaataaatgg aagtatgaga ttgaataaaa
     8461 agaaaagcag ataaaacggc aatactttat accgctcttc acgaatggca aagaagccac
     8521 aaataacgat atatacgtca atgataaaac gcaaaatcca agctgctgtc ataaaactgt
     8581 tcaaatgccc atggaggtta aaataaccat acatacgaat ataaaaatag ctgaatatta
     8641 aatgtgctaa tgaaagaaaa gcaatactaa gtgtaaaaaa aagatgtttc ttctgcatga
     8701 ttgttccagt cccttactaa gatagatagt ctttattata ccacgtgtga ggggagaaat
     8761 aaaaagccgt tttttagtag atatgaaaca aaacaaaaac ccgcatttat tcaacctatt
     8821 catcctttta aaggatagat tgaacgtaga tagaagtcgt ttatccagtc tttttcattt
     8881 tgaaaaaaag ctaacagcat gcttttttta tggtatacta gcaaggaaac gaattcgcat
     8941 catgcgataa aggaggaaga gccccgtggc agaaaaggaa actttttata tcactacgcc
     9001 aatctattat ccaagcggca aacttcatat cggcaactcg tatactacga ttgcatgtga
     9061 cgcaattgct cgttataaac ggttgatggg ctttgatgtg ttttatctca ctggagtgga
     9121 tgaacatggt caaaaaatcg aaacaaaagc agaagaacta ggtgtaaaac cgcaagaata
     9181 tgtagataaa atggcggctg atgtcaagaa attatggaaa acgcttgata tcagttatga
     9241 taaattcatt cgcacgacag atgattacca taaagcagct gttcaaaaaa tatttgatcg
     9301 tttattagaa cagggagata tttacttagg tgaatatgaa ggctggtact ctgtttctga
     9361 cgaagaattc tttacagaaa cacaacttgc agaagtgtac aaagatgacg aaggtaacgt
     9421 gatcggtggt aaagcgccaa gtggacacga agtagaattg gtaaaagaag aatcatactt
     9481 cttccgcatg agcaaatacg cagaccgtct attggaatac tatgaagaac atcccgaatt
     9541 catccagcca gaatctcgta aaaacgaaat gatcaacaac tttatcaaac caggattaga
     9601 ggatcttgca gtttcacgaa caacttttac ttggggagta ccagtcaaga gtgatccgaa
     9661 acatgtggtg tatgtatgga tcgatgcatt atccaattat attactgcac taggatatgg
     9721 ctccgaagat gaaagtctat tcgaaaaata ctggcctgca gatgttcaga tggtcggtaa
     9781 agaaatcgtt cgctttcata cgatctattg gccaatcatg ctgatggccc ttgatttgcc
     9841 tctacctaaa aaagtatttg ggcatggctg gctattgatg aaagacggaa aaatgtctaa
     9901 atcaaaagga aatgtcgttt atcctgaaat gcttgtggaa cgttatggtt tagatgcact
     9961 ccgttattat ttgcttcgtg ctgttccgtt tgggtcagat ggtgtcttta cgccagaaga
    10021 ttttgtttcc cgtgtcaatt atgatctggc aaatgatcta gggaacttat tgaatcgaac
    10081 gattgctatg atcaacaaat attgtgatgg actcgttcct gactatgcct cacttgtcac
    10141 tccatttgat agtgaactgt ctacgacggc tgctaacgtt gtcggacgtt accatgatgc
    10201 aatggaaaaa atggaattca atacagcatt ggctgaaatc tgggtgctga tttctcgtgc
    10261 aaacaaatac attgatgaaa cagaaccatg ggtattagca aaagacgaag aaaagaaagc
    10321 tgaattgaat agtgtcatga tccatttagc tgaatctttg cgtatcgcag cgatattact
    10381 tcagccaatc atgacagaaa cgccagagaa gatcttcaat caactaggat tggactctga
    10441 aacaatgaat cttgaaggat tacactttgg cgaatttcct tcaggaacaa aagtagttgc
    10501 aaaaggtaca ccaatcttcc cacgtttaga tatggaagaa gaagttgctt ttattcaaga
    10561 aaaaatgtct gaaggtactc aaacaaacga agatacagtc aaatgggatc cagaagaaac
    10621 agaacttgtt tcaacgaaag aaaaacaaat caaatttgat gtatttgaaa aagtagaact
    10681 aaaagtagca gaagtcatca actgtcaaaa agtagaaggg gcagataagt tgctgcaatt
    10741 ccgactagat gctggagaca gccaagaccg tcaaatcttg tcaggaatcg ctgaatttta
    10801 tcctgatcca agcgaattga tcgggaaaaa agtcgtcatt gtagcaaatc tgaaaccacg
    10861 aaagatgcgt ggacaaatca gtcaaggaat gatcctttct gcagaagcac cagatggctc
    10921 tttacaagta atcgaagcac caaaatccat gccaaatggc tcagaaatcg catagaaatt
    10981 caaagggggt atgacactag ttgtgtcata cccccttttt ccgaaatttg agaagcaatt
    11041 ttcgtaaatt ccttcttatt acttgaagcc aatcatataa tacagaaaca gagaactatc
    11101 caaaataaat cctagaaaaa gaagtacgag cttcgctgat ttttgttaat ttagaataat
    11161 actaatagga atcgtaaaaa cagaaaaaaa gcataatgat aaagggtcgg gcagatttta
    11221 attgattcat ctggtactct tttttattgt ccttacattc ataaagtttg aatgcaatga
    11281 ctttattaca tctatattta tatctaacag tagcgttgat gtgttaatta taagttctat
    11341 cactttatgt tcaattagtt tgtgctctta tatgtgtgat tgaaaatata gataaaaaaa
    11401 caatcatcgg ctttgacttc gatgattgtt ttctttattt tgtaaaaatc ctatcacttg
    11461 attttatggt aaccttttat ttttgtcttc aaacggcttg gtaagagagc agtaagcagt
    11521 gcaaatggga tcgaaaagac ataccagaaa atcgtacgtc cgataccata atgttgatag
    11581 agctttgtca acatcacgtg taacaagatc aaccctaaaa gcaaggagcc agctataaac
    11641 caatgattat cccaatgcat gacttcacta agtgccatca ctccaaaagg atagaaataa
    11701 aaatagatcc cgactaaggt catccgccag ttctttgtaa atgttccgat attcaataaa
    11761 aagaaaatcg ctgaaagcaa aataccgata ggcggaaaaa gcccaaccaa aaaggaatac
    11821 aacatccccc acaagatcat cacaggacct ttgatcaatg caacgattgc gatgaacaaa
    11881 gtcagcaata ttagtttttg tgataacgtt gcaacagtca ttaatccaaa taaaattaat
    11941 gtgaaccaat gccaatggga ctgctgttgt tctttacggg aaaaatcgac ccggttggat
    12001 aaataatctg ctaatttcgg ataagaacgc ttaaaatcca taaactgttc ctccttcgtg
    12061 gaaaaggtag gtttagtata cgaaaaataa aaaagaaata catccgtctt aagagggagt
    12121 ttttcactga aagtggagaa aaaagaatgg taaattaaaa tattctcagt tatactgaaa
    12181 attataccaa atttcaaaga agaaagagga tgggaatgaa aggcaaacta aaacgagaaa
    12241 tcaatctgtt tggcgcatta gctaccgtta tgggaaccgt gatcggtgca ggcgtcttct
    12301 tcaaaactgc tgctgttact gctagtactc agtctacaag tttgacattg ctagcttggc
    12361 ttttgggtgg tttcttaacg atctgtgctg gattgacagt ggctgaatta gctacagcaa
    12421 tcccggaaac tggtggagct gtaaaatata ttgaagctgc gtatggaaaa ttgcccagct
    12481 ttttattagg atgggcgcaa agcttgattt actttcctgc taatattgct gcattatcga
    12541 ttatctttgc gacacaattg acgaacttat tacaattatc taccgattat cttttgttga
    12601 ttgcagtgat caccgctgtt tctgtgacag ggttgaattt gttagggaca aaagtcggtg
    12661 cttctgtaca atcagtgacg ttgattgtta aattaatacc aattgctgtg attgtcatat
    12721 ggggattatt gaccccagga caaggaactg tccagttatt tccaatcgaa gctggaaaag
    12781 atgttacttt tgtcgaagga ttgagcagtg cgttgttagc aacgttgttt gcttatgacg
    12841 gctggttagg tgtcggtgca atggcagggg aaatgaaacg gccggaaaaa gatttgccta
    12901 aagcaattat tcttggtttg agttttgtaa cagtcgttta tctattgatc aattttgttt
    12961 ttttgaaaac tttgccgatt gatcatcttg ccggtaattt gaatgcagct tctgaagcat
    13021 cagacgtcat ttttggagga atcggtggaa agctggtcac tattgggatc ttgatctcgg
    13081 tatacggtgc attgaacggt tatacactaa ctgggatccg cgtgccttat gcgatggctt
    13141 tagaagacga gctacctttc agtaaacagc tgacaaagct ttcaaagaag tttacggttc
    13201 cttatgttcc tgcggttttc cagttagcag tagcttgtat tatgatgagc cttggttctt
    13261 tcgacttttt aacagacatg ttgatttttg tgatgtggct attcagttta ctgattttta
    13321 tcggtgtctt tattttaaga aaaaaagcgc cggaattgcc acgtccatat aaagtaccac
    13381 tttatccaat cgttccgatt attgcgattc tcggtgctat tttcatttta ggtatgacga
    13441 tgatgacgca aacgaaatta gcattgatcg gtataggtgt taccttgata ggtatcccag
    13501 tctattatca aaaaaagaaa aaacgctcag aagagtaaaa tatttcaaca acagacagaa
    13561 aaagtacgaa ctaggtcctc tagatcttcg tgctttttct atttcaataa aacaagttct
    13621 tctattttga tttttgctac ttctgcaagt agtgcttatg tgctaaagta agaatgaaaa
    13681 ggagagataa acatgatatt tgattctcat acccatttaa atgctgaaca gttcaatgaa
    13741 gatattccag aaacgattca gcgagcacaa gaaatggggg taacgaaaat ggcggtcgtg
    13801 ggcttcgata cgccaacgat tgaaaagtct ttgcaattaa gccatgacta tccaaatata
    13861 tacagtatta tcggctggca tccgacagag gctggcagtt atacgaaaga aatcgaaaat
    13921 aaattacaag aacaattgac tttaccaaag gttgtagcat taggggaaat tggcttggat
    13981 tatcattgga tggaagaccc gaaagaagtc caagataaag tatttcgcag acagattgct
    14041 atcgcaaaag aaatgaacct accaatcagt attcatacta gagaagcact tgaagacacg
    14101 tatcaaatcc taaaagaaga agatgttcga gatatcggtg gaatcatgca tagtttcagt
    14161 ggtgattatg aatgggcaaa acgatttctt gacttaggga tgcatatttc atttagcggt
    14221 gtcgtcactt tcaaaaaagc attagacgtc caagaggctg cagcaaatgt accaatcgaa
    14281 cgattacttg tggaaacaga tgccccttat ctggcaccag ttccttttag aggaaagcga
    14341 aatgaaccag gctatactcg ttatacagta gaaaagattg ctgaacttcg gaatcttccg
    14401 acagaagaag tggctgccca aacatggaca aatgcgcacc gattatttgg tctaagcgaa
    14461 catggataag atgaagatcc aagagattat agtcgttgaa ggaaaagacg atacaagaag
    14521 gctgcgggaa gtagttgatg cagatacgat agaaacaata gggtctgcaa tcaatgaaga
    14581 gatcctgatg caaattgaac atgcccaaga gactcgtgga gtgatcgttt ttaccgatcc
    14641 tgatttttca ggagaaaaaa taagaaaaat cattatggaa gctgttcctc aggcaaaaca
    14701 tgcttttttg ccgcgcacac aagctaaacc aaagaaaaag ggaagccttg gtgtcgagca
    14761 tgcaagtgat gctgctattt tagaagcatt gaaaaaggtc gtgacgccag acatttccca
    14821 aacggctgtt tctgaaatca ctagacaaga ccttttagac tatggtttaa tagctggggc
    14881 acaagcaaaa gaaaaacgag aaaaactagg tgacgagttg cgcatcggtt acacgaacgg
    14941 caaacaattg gaaaaacgtt tgaagatgtt tcgtattacg ccgaaagaac tcgcccaagc
    15001 aatgaaaata gtggaggaac aacatgagtg aatatcgtga aattgcgaca ccctctagaa
    15061 caaaagagat tttattgaaa catggttttt catttaaaaa aagtttagga caaaattttc
    15121 tgactgaacc aaatatttta aggaaaatcg tagaaactgc gcaaatagat gaacatacga
    15181 acgtgatcga agttggacca ggaatcggtg cattgacgga acaattagcc aagcatgcaa
    15241 aacaagtcgt agcatttgag atcgatgatc gtttgatccc tgtattggct gatacgatgc
    15301 aaccctatga caatgtgact attgtacatc aagatatttt aaagaccgat ttgtcaacag
    15361 ctgttagaga aacatttcat gaggagcttc cgttaaaagt cgtagcgaat ttaccttact
    15421 atattacgac accgatcatg atgcattttc ttgaatcaga tttggttgtg gatgaactag
    15481 tcgtcatgat gcaaaaagaa gtagctgatc gtatctctgc tgaaccagga acgaaagctt
    15541 atggttcatt atcgattgct gttcaatatt atatggaagc cagtttagca ttcatcgtac
    15601 caaaaacagt atttgtccct cagccaaatg tagattcagc gattttgaaa ctgacaagaa
    15661 gagatatacc ggcagtcgaa gtgacagacg aaaaagcatt tttccgattg acaaaagcgg
    15721 catttcagca aagaagaaaa acgctttgga ataacttgca gcattcgtat ggcaaagatg
    15781 accagacaaa agcatggtta gcaaaaagct tggaaacagc tggaatcgat ccaaaacgtc
    15841 gtggggagac tttgtcatta caagaattcg ccgcattaag caatgcgatg tcagaaaata
    15901 tgcagtaaaa aaagaggttg tgacagaagt tgtgaaaaag ctgtcctgca tcaagtaata
    15961 agaacagcca aacaaaaata gttcctcatg tttttcgttt gtttgggctt gttaccgcag
    16021 atgcaagctt ttgaacaccg tttaagaaag aggttgtggg cctgccgttt aactctaagt
    16081 cacaaggaac aatttcaaaa aacagcttct catgtttttg agttagttgg tcttattact
    16141 tgatgcaagc ttttgaccac tattatgccg aacaaggaga tgtgacacaa attttgtcac
    16201 atctcctatt tttgtttctt gatcaaaaaa ttcttatact tgatactcac actcattttg
    16261 tatggattta gaaaagaata tgtgtcttga cagttattag taagtgagtt atactaagtc
    16321 gacatttatg tgtcaagaca tattaggaga gagaaaaaat gagaaaaaat tcagttcgaa
    16381 aactaacgat ttctgcttta ttgattggga tgggggtcat tatcccaatg gtcatgccta
    16441 aaatcatgat tccaccagct tcctttactt tggcgtctca tgtgccatta tttattgcga
    16501 tgttttttac accaggagtt gcagtggctg ttgcgctagg tacgacattt ggtttctttt
    16561 taacaacgcc ggttatcatc gcattgcgag cattgtcaca tcttgtgttt gcgttgattg
    16621 gggcttggta cttgcaaaag catccgagta tcgttttaaa aaatggacag tttacttttg
    16681 ctaattggcg tttccaaggg ttcaattttg tgatcggtgt gattcattct ttagcagaaa
    16741 tgctagtagt cagcatcttt tactttattg gtaatatgcc tgaaacatat tattctcaag
    16801 gatattttta tacagtattc atcttgatgg ggattggtgg gcttatccac agtttagttg
    16861 attataatat tgcttatttt atagcaggaa cactcagcaa atattttgat atccccgtat
    16921 ttacggcagc aaaaaaagaa aatcggaaag agtcgattca agggtacgcg aagaaaacga
    16981 catattagac atgatctctc aaaaaaaaaa ctctcatcta gtaatggata ataaatcctc
    17041 taagatgaga gttttttgtc ctaaaaagag ctgcaccaac atcttctggc acagctctaa
    17101 cagggggcaa tttatggaag tttgatgact tgttctgttt ctttatcaaa gaagtggcct
    17161 ttgctgatgt tgaaggcaag gtcgactatt tcgcctggtt tatggaaatc tcttgcatct
    17221 acttttgaga tgaactcagt agaaccagtt tttgtgtaaa gcatcgtttc agctccaagt
    17281 aattcggaaa cgacaacttc tgatttcact acggcttcag gtgaagcttc aagggctact
    17341 tgttcactgt ggatgtcttc tggacgaata ccgaagataa cttcttttcc ttcataacct
    17401 ttttcaacta atactttgtt ttttccttca ggaatacgaa gcttaagccc atgtccatct
    17461 gtgataacac catttttcaa tgtcacatta aagaagttca tggcaggaga tccaataaag
    17521 ccggcaacga acatattgac gggtgtatca taaacttctt tcggtgtacc gatttgttgg
    17581 ataaatccgt ctttcatgat aacgattcgg tcagccatag tcattgcttc tgtctgatcg
    17641 tgagtaacat agatggtcgt tgtttcaaga cgctggtgaa gtttggcaat ctcagcacgc
    17701 atggctacac gtaatttagc atctaaattt gataatggct catccattaa gaagactttt
    17761 gcatcccgaa cgattgcccg tcccaatgcc acacgttggc gttgtccacc agataaagca
    17821 gctggttttc gtttcaagta ttctgtcaga cctaagatct ctgctgcatt gtccacacgt
    17881 tttttgatgt ctgctttatc atatttacgt aatttcaagc caaaagccat attatcaaaa
    17941 acagtcatat gaggatacaa tgcgtagttt tggaaaacca tggcaatatc acggtcttta
    18001 ggtgccacgt cattcatgac ttttccgccg atatttagtt ctccttcaga gatatcttcc
    18061 agtcccgcga tcattcgcaa agtagttgac tttccacaac cggaaggacc aacaaaaacg
    18121 ataaattcac ggtctttgat tgatagatta aaatcagtta cagaataatt ttctgcattg
    18181 tcatattttt tatatacgtt tttcagagcc atttctacca taatacgttc cacactttct
    18241 tttttagttt tattaactat ttaaagtata aatgaaagcg ttaactaact tcaatgtcat
    18301 gttgaacaag aattttttct tttttttgtc aatgtgctag ttttaagctt taataaatat
    18361 ccataagaaa taaaaactga tacactggag taaataagta tgttaacaat atcaaaagga
    18421 gtgtctctta tgaaaatcgc tttgatcgct catgaccgaa aaaaacctat gatggtgaaa
    18481 ttagcaacag cgtataaaac aatattggaa aaacatgaac tttttgctac cgggacgact
    18541 gggcagagga tcacggaagc aaccggcttg ccgatccatc ggtttaaatc cggaccgtta
    18601 ggcggggatc agcaaattgg agccatgatt tcggaggatc agttagattt ggtgattttt
    18661 cttcgagacc cgctggctgc acagccccat gaaccagatg taacagcact tatccgttta
    18721 tctgatgtct atgagattcc gttagctacg aatatcggaa cagctgaggt gctactaaga
    18781 ggactggaag ccggcttcct agattttcga agaatcgtcc atgaaataga caagcatcca
    18841 ctttcatttt gatggataag cgtatggaaa aataacgttt aatgttcgcc tatatgtagc
    18901 tataaaagct aaacaaactg ataatatgcg atataatacg aacgtattgg ataatataaa
    18961 caataatatt agtttttcgt atttgcagta aatgtgaaaa tatgctatag tttctgtggg
    19021 aaaagatccc aaaaataata cagagtagaa aataaagcgt taagtgctaa gaggatggga
    19081 tgttgcctct tggacgaagg acgtaatgtt cgcggtttta ttttcgcatt cgctgcatga
    19141 tacgatgtag cagctcttaa aggagatgct aaaaatgaag aaacaccggt tagacgcacg
    19201 tatgattgct attatgggct tattgattgc attgatggtc actttgtccc gactggtagc
    19261 aatcgagacc ccatttatta aaatcagtgt cacatttatt ccacaagtga tcatgggcat
    19321 tctttttggt cctttttggt cagggatagg cgcagtttta gctgatttag taggaatggc
    19381 cctcttctcg aaatctgcat tctttattgg atttacattg aatgcattta ttgaaggagc
    19441 aatctacgga ttctttttct atcgaaaaga gatcacttgg aaaaatgcca ttctggctac
    19501 gcttagtgtc acgctgatca tcaacttatt tttgacacca ttatggcttg cgttgatgta
    19561 ccatgtgcca ttgttcagct gggtcgtttg ggcgccccgc ctgttaaaaa cagtaatatg
    19621 gttaccgatt caatcaattg ccatttatta cgtaggacgt agtattcctt acaaaaaaat
    19681 tttaagaagt ttagcgatcc acgctaaata aaaaacaagc agtcttccca tcccagaaga
    19741 ctgcttgttt ttattgtgct gtgaattgtg cttgatagag ccgtcggtaa taaccatctt
    19801 tttgaagcag ctcatcatgg gtgcccattt cgacaatagc tccctgatcc atcactaata
    19861 tctggtccgc atttttgatt gtggacaggc gatgggcaat cacaaaactg gtttttcctt
    19921 ccatcattgc taagaaggct tgttggattt ttttctctgt caatgtatcg actgagctgg
    19981 tagcttcatc taagatcaac atagggggat tactgatcat cgttcgagca atcgtcaaca
    20041 gttggcgttg tccatctgat atctttaacc cttgaccgcc aatcttagta tctaattttt
    20101 ccggcaaacg ttctacaaat tcatacatat aagaggcttt tagggcatca ttgatttctg
    20161 cgtcacttgc ttccggattc ccaaaacgaa gattttctcg caatgtactg tcaaatagcc
    20221 aagtatcttg taacaccatt ccaaaacttt tacgtaatgt atcacgttgg atagagcgga
    20281 tgtcatggtc atcgactgta attgttcctt ttgttgtgtc ataaaaacgc atcagcaaat
    20341 taacaagtgt ggattttccg gcccctgttt ttcctactat agcaatggtt tgacctggat
    20401 aagcagtaaa attgaaatct tcgatcaaac gctgcttggg gtcataagaa aaagaaacat
    20461 gatcaaaaga gacttttcct ttgatatttt tgggttccaa ggcataggca tctggcaaat
    20521 caggcttttc ttctggctga tcgatcaaat caaatgctcg ttctagacca gaaaaagcgg
    20581 tttgcagttg tgtcgtgatt ccagaaagtt cgataaatgg tttagaaaac tgacttgagt
    20641 agatcgtaaa gctggaaatc acaccaacag tgattgacga accactcttc aatgccaata
    20701 ggccgccaac taaaccaatc gataaataag caaggtgatc tacaaaacga gaagacgggt
    20761 ttgtcaagga agaagaaaat tgtgcccgtt gtcctttttc atatagatca gcatttagca
    20821 gttcaaattg ctgttgacta gcttcttctt gctgaaaagc tttcacgatt ttctggttac
    20881 cgattcgttc ggtcacaaag cccgagatat ccccaataat cctttgctgt ctatcgaaat
    20941 ttgtttgtga agcacgagca acgatccaac tcacaaggaa aataattggt gtagaacaaa
    21001 gaacgaccaa tgtcagaagt ggacttagac gcatcataaa aataaacgaa agaatgatga
    21061 ttgccactcc agaaaataat tgattgaagg ccgcagaaat tgcaatggaa atattgtcca
    21121 tatcatttgt aaatcgactg acgatatttc cgtgggagtt ttgatcataa taacgaagtg
    21181 gcaatcgatt cagatgagca aatgcatctt ttcttaattt tgcaacggat aagtaagcta
    21241 accgattgct taacacttgg atgagccatt gactgactac gttgattaaa acgataccac
    21301 cgaatagaat aagtaaatgg aacagtcgat tgaagcggac ctgtccttct ccaatcattt
    21361 gatcgatact ttgaccaatt tgtaacgtca tcacaactgt tgcaatccca ttgatgattc
    21421 ctagtatcac agctagccaa atgtcttttg cataggcagt aaggtaagga atgaatcggc
    21481 ggagcgtctg caaggataat ttttttactt tcataactat tcttcctcct gtgatgcgac
    21541 gatttcctga tattctgctg attgagcaag taattcttca tgggtaccct gagcagcaat
    21601 cttaccttga tccatcacta agatatgatg tgcttcttga atagagcgga cacgttggga
    21661 gataataatc actgtactgc caagataagt ctgtagcgcc ttacgcaaat ccaagtctgt
    21721 ttgataatcc aacgcactta aagagtcatc taaaatcagt aatgctggct tggcaatcaa
    21781 agctcttgca atcgtcagtc gctgtttttg tccgccagag aaatttttcc cattttccat
    21841 gacttttgta tctaaaccat ttggaagctg acggacaaat tcttctgcct gagcgatttt
    21901 cagagcttcc caacattctt tttctgtggc gtctttctta ccccattgaa ggttttcacg
    21961 aatagtcccg gtaaatagta cagaagtttg gggaacaaga gcaatttgct gacggagatg
    22021 atccagctgc caagaacgaa catccattcc gtccattata actgtaccgg aactgacatc
    22081 atagaaacgc ggaatcaatt gtgtcaaagt cgttttcccg cttccggttg gaccaatgat
    22141 tccaaggact gtatttcgct tcagctgaaa tgtaatattt tggagagaaa gaccagaatc
    22201 aggagtataa cggaagtcaa catggtcaaa agcgataatt gcttcagaag aaaaatcggg
    22261 agtagtactg acctcagctt caagaatcga attttctgtg tctagcacct ctttcacccg
    22321 atttcctgaa gcttcggcac gtgtgaagat cacgacgagg tttgaaacaa cgatcagtgc
    22381 caaaagcatt tgattcatat aattgatcaa agcaagaact tgtccttgtt gcaagctacc
    22441 aatatttact ttgatcccgc caactgtcag caaacagatg atcccgacat tcatgatcaa
    22501 ggttgtagca ggtgataata gggcagagat attggaaaca cgtaaataat tgttcgctag
    22561 atcatttgtg cttttatcga acttctctgt ttcttttttt gtctgcgcaa atgcgcggat
    22621 gacacgaaca ccgctaagat tctgactaac gtgttcattg agctgatcta gtttttcttg
    22681 cactttttgg tagagaggaa cggttttttt gatgataaaa aataaaatga ttgagaaaat
    22741 aggcaataat gctaagaaaa acagacctac ttcccagtcg atcacaaaag ccatgaccac
    22801 tgacccgata cttaaaaacg gtgctctgat caataaacga atgaccattg ctaaagctag
    22861 ctgaagctga ttgatgtcgt ttgtcattcg ggtgatcaag gtatctgttc caaaatgatt
    22921 caactcgttg tgagagaaag tattgatttt tttgattagc tgattacgta actctgtacc
    22981 aaatccttgt gaagctactg aagcatagta ttggcaaatg atagcacaaa tcagaccaat
    23041 cacagacata gcgaccatcc ataagcccat ttcaatgatt tttcctcggt cattcatttt
    23101 tagtccatta tcgatcaagg aagccatcat taaaggcagg acaagttcga agacagcttc
    23161 aagaaatttg aagaacgggc ccaaaatgat ttgcttacga tagtcttttg cataacgtaa
    23221 taactgaatc atatgattcc tcctattatt tatttgtcca caattgaaaa aacaagtact
    23281 accacattgt agcttaggaa atttagaatg aaaagagaac aagccttttc ataaatgaaa
    23341 gttttattat gaagaagctt tagaaattga atagactatt ttttttaaaa aaaagaaaat
    23401 gagaaaacat gccagt
//
LOCUS       DAZULO010000034        23322 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000034 DAZULO010000000
VERSION     DAZULO010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.035"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            237..791
                     /locus_tag="ACG8OA_001354"
     CDS             237..791
                     /locus_tag="ACG8OA_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4976152.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(964..1401)
                     /locus_tag="ACG8OA_001355"
     CDS             complement(964..1401)
                     /locus_tag="ACG8OA_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4976153.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1449..2396
                     /locus_tag="ACG8OA_001356"
                     /pseudo
     CDS             1449..2396
                     /locus_tag="ACG8OA_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            2667..3102
                     /locus_tag="ACG8OA_001357"
                     /pseudo
     CDS             2667..3102
                     /locus_tag="ACG8OA_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            3118..3951
                     /locus_tag="ACG8OA_001358"
     CDS             3118..3951
                     /locus_tag="ACG8OA_001358"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4976154.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            4196..5221
                     /locus_tag="ACG8OA_001359"
     CDS             4196..5221
                     /locus_tag="ACG8OA_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4976155.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5359..5484
                     /locus_tag="ACG8OA_001360"
     CDS             5359..5484
                     /locus_tag="ACG8OA_001360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976156.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            5509..6624
                     /locus_tag="ACG8OA_001361"
                     /pseudo
     CDS             5509..6624
                     /locus_tag="ACG8OA_001361"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            6707..7603
                     /locus_tag="ACG8OA_001362"
                     /pseudo
     CDS             6707..7603
                     /locus_tag="ACG8OA_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            7619..8443
                     /locus_tag="ACG8OA_001363"
     CDS             7619..8443
                     /locus_tag="ACG8OA_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4976157.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8475..9785
                     /locus_tag="ACG8OA_001364"
     CDS             8475..9785
                     /locus_tag="ACG8OA_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFS4976158.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9933..11192
                     /locus_tag="ACG8OA_001365"
     CDS             9933..11192
                     /locus_tag="ACG8OA_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4976159.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11202..12074
                     /locus_tag="ACG8OA_001366"
     CDS             11202..12074
                     /locus_tag="ACG8OA_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4976160.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12086..12916
                     /locus_tag="ACG8OA_001367"
     CDS             12086..12916
                     /locus_tag="ACG8OA_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4976161.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12956..15139
                     /locus_tag="ACG8OA_001368"
     CDS             12956..15139
                     /locus_tag="ACG8OA_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS4976162.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGNELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15200..16420
                     /locus_tag="ACG8OA_001369"
     CDS             15200..16420
                     /locus_tag="ACG8OA_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFS4976163.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16417..17877
                     /gene="gtfA"
                     /locus_tag="ACG8OA_001370"
     CDS             16417..17877
                     /gene="gtfA"
                     /locus_tag="ACG8OA_001370"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFS4976164.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17954..19060
                     /locus_tag="ACG8OA_001371"
                     /pseudo
     CDS             17954..19060
                     /locus_tag="ACG8OA_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19382..19591
                     /locus_tag="ACG8OA_001372"
     CDS             19382..19591
                     /locus_tag="ACG8OA_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS4976165.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19584..19733
                     /locus_tag="ACG8OA_001373"
     CDS             19584..19733
                     /locus_tag="ACG8OA_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFS4976166.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19887..20552
                     /locus_tag="ACG8OA_001374"
     CDS             19887..20552
                     /locus_tag="ACG8OA_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4976167.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20836..21921)
                     /gene="xerS"
                     /locus_tag="ACG8OA_001375"
     CDS             complement(20836..21921)
                     /gene="xerS"
                     /locus_tag="ACG8OA_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFS4976168.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22255..22488
                     /locus_tag="ACG8OA_001376"
     CDS             22255..22488
                     /locus_tag="ACG8OA_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFS4976169.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22485..22892
                     /locus_tag="ACG8OA_001377"
     CDS             22485..22892
                     /locus_tag="ACG8OA_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS4976170.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT         7989 a         3487 c         4440 g         7406 t
ORIGIN      
        1 tctacggata actgcccgtc taaaatttaa ataagagctt agaatcttca tataagtaag
       61 gttaattttt ttgtacaaaa aagtcggaat attctgagtt actgaaatga tataaaatca
      121 taaactaaat cccttgattt tattgacttt caaaagttta tatatataag ttaatgatta
      181 tggtaaaata tatacataat cattaactca gaatattttt aagaagggaa acaatagtga
      241 gctataatgt acaaccgtta aggacaaatg aagagataca agattttcag ttttggctaa
      301 gaagaacaag taatccggaa agagatagtt ttttattttt gtttggaatc aataatgggt
      361 tacgtatgtc agatattata ggattaaaag ttggagatat acgaggcaaa agtaagccta
      421 ttattgttga acataaaaca ggtaagcgaa aaccgatttt tatagataat ttgagagaag
      481 aaattttatt atatactgaa ggtaaagaag aaaatgattg gttatttccg agtcggcaac
      541 aaggtcgaca tataacaagg gatcgtgtct atcagatata tgcagatata gcagaaaaat
      601 taggacgaga tgatatagga acacatacat taagaaaaac ttttgggtat cactactaca
      661 agaaaactag agacattgct accttaatgt ttatttttaa tcatagctcg caagcaatta
      721 caaaaaggta cattggtata actgaagatg aaataggagc atcacttcgt ggatttaaac
      781 taggtgtata atttaaaaat ttttataaac agtaaagaca aagattaaaa gctattttta
      841 tctagaacga ttttatttca taaagttcgc ggaataaaag ctggggttac tggtacttgg
      901 tataggttcg tgttatataa actaagtaat taaataacat ttttaggttg gaaaaggatg
      961 aaattaaaaa aagagaatca atacggtctc aaaaacgcaa acgctgcgga ataagaaaaa
     1021 tactgataac tcaacgttta gcagactatc taatagggat agtctgcttt tttcgttttt
     1081 gaccatactt ttgaccttct gcaaacttcc ttgcttttcc tctgtagaac tcatgaaatc
     1141 tgcaaaacga attgccgttt cctctgtctg ctttttcgcg atatgggtgt atatatcatc
     1201 catagtggtt ttaatatctg tatgtccaag tcgaacatac acttctttga cagacgcacc
     1261 agattcaaag agcagactac aatgggtatg acgaaaacca tgaagtgtga tatgttttaa
     1321 gttattctct ttaatgatga ttttcaaatt gtgatttaca tagtctaaat aaaggggctt
     1381 attttcttct gatgaaaaca aaagttgttc accatctttt ataggtttga ttctcatttt
     1441 tagatattat gcccgcacgt attcatcaca tttctattgt gaatcgcttt attcgaccga
     1501 catttgactt ttatcataat atactcggtt taaaattact aatgaaaaca attaatcaag
     1561 acgatcatac catgtatcac ctgttttttt cagataatca ccaccgtgtg ggaactgaac
     1621 tgactttttt tgaagtacaa gaagggaaca accagttttt tggaaccaat acgattgaac
     1681 gaacgatact aaaagttcct tccgaagcat cattgcattt ttgggaaata tattttgaaa
     1741 cacaaggcgt ttgccattat ggaatcgaat cattttctgg acgacctatc ttacgttttg
     1801 aagggccaga tgcaacacga ttagctttag ttcccttacg cgaatttgaa gatattgatg
     1861 attatttccc gtatgtcaca gaccagatcc ctacagagca tgccatttta ggaattgatg
     1921 ccattcaact acgcgtacaa tattccgatg cgactgaacg agagttattg tcggtaggct
     1981 ggtagcacaa agaatacgtc agtttctttt cgggtcatca acgagtgaca atcttagaaa
     2041 atcaagacac tcagttctat caagaagttc agattattca agatcgtgag aatccagttg
     2101 ccattgaagg gattggtagc gtccatcatg ttgcgtttgg cgtagaaaat aaatctgatt
     2161 tacaacgcat cgacaaacaa ttacaagagc gcaattttat aaattcagga ataaaagatc
     2221 gtgaattttt tgtatcactt tattatcgtg acccaaatca attattgatt gaaattgcta
     2281 caggggaagg aaacctagat gcaaaagcgt atgaaaatca aagtccacgc tttgaagaaa
     2341 ttcctttatt tttaccgcag tacttaaatt tcattcgtga acaagtggag caataattag
     2401 ctaatcaaat ccatgacgaa gagtaagagg ttgactcata agtcaaaaaa taaagaaaaa
     2461 cgcatcaaat ccattctaaa gaatgttgat ttgatgcggt tcctattttt ataaactatc
     2521 attttcgact aataaaacta cttatgagtc agcctctctt tttttcttga tttttatacc
     2581 tctttctagg taaactaaac atctgccaaa aataccgtgt ctcattgaaa cattctcaac
     2641 tatttcaaaa taaaggggag taccaaatga acgagtatat tgaaattatc cacgcaagag
     2701 aaacaactta aaaatgtcag tctcaaaatt cccaaaaata aaattagtat ttttaccagc
     2761 gtatctggtt caggaaaatc atcgattgtt tttgatacta ttacccaaga agctggtcgt
     2821 caactgaatg acacctatga tagttttacc cgtttatttt tacctaaata cagtcgtcca
     2881 gaggttgatg agattgataa tctttctacg gcgattgtgg tcaatcaaaa gaagattggt
     2941 ggcagtgcgc gctcaacgtt agggacgatt actaacatca atgcgttatt tcgtgtgttg
     3001 ttttcatgtt ttgcaacacc tagtttagga tatgcaaatg cttattcatt taatgatcct
     3061 agtggaatgt gttctgtctg tcagggaatc ggtaagacga ttgcattttt aataggattg
     3121 ctttattttt cggctattga taatgctgat tttgtatttt ctgatccatt taccgcagaa
     3181 gttttgtctg gaattgagga acaggcaaag aatcatggtt attttgtatt ggtgcataat
     3241 gttaattcag cagaagatgt taaaactatt cagagaaatt ggcgttttga ggggttcatt
     3301 gttgttggtg tacgtgtaaa agaatttttg gaattaaata acgtttttga tggtcctgtt
     3361 gtttatattg atacgcatct ttcaaaagag acattaagag aacttgaaca acaaatgaat
     3421 cgtttatttg taaatacaaa tgattaccaa gctagtacat tggcagtgga acatttgatt
     3481 cattatggtc atgagaaaat agcgtttctt tcttttgact acattgtagg tgaaccaagt
     3541 gttattcaag aacgacatca agcttatatc gatacattaa agaaatatca tttaaccttt
     3601 aaatcgcatt ggatatataa caactctgaa tttgagcgaa tttatcaaga attagctgaa
     3661 tttactgcaa ttgttgttac tggagattat ttagcggcta aattagtaaa atttttaaaa
     3721 aataagaata aacgagtgat ggaacaatta tcgattattg gatttgatga tattactttt
     3781 gcagaattaa tggatcctgc gttgacaaca atacgactaa atccaggtaa caagggggcg
     3841 attgcttttc accaactgat tggactggcc aaacaagaag aaataaaaaa tcaagtacta
     3901 ctattagagg gagaattgat ttctcgagac tctgttcaca aaatcaaata aaaaagctct
     3961 gtgaaataag tatagttaac aataaaatat ccgaacgata gagagaatga actgtgatct
     4021 agcagttcct cgtcgcaaat tcactagaat tagcattatt tactctaaat tgttcggata
     4081 ttttttcttg tttttaatta tgattacagt gcctattttt aacgaaattt gcttattttc
     4141 aaaatgtatg attatatgtt agtaaacact ttcaaatatg aaaagaggag ctattatggt
     4201 aggaattagg gatgttgcaa aacgggcggg tgtgtccatt gcaacagtat cgcgcgtgct
     4261 aaatgaggat ccgacacttt cagtaaccga tgaaaccaaa cgaaagattg cagaatcggt
     4321 taatttttat cattatgtca aaaaaagtcc cgttgcaaaa aatttaacaa atattgcatt
     4381 aataacgaca gtttctgaag tagatgagtt ggaagatccc tattttcgaa cgattcgcag
     4441 agggattcaa gtcgaagcag aacaaagcaa agtcaatttg aagaaaatta ttcgtttgtc
     4501 agaaacaccg ttggagcaag acgatttgct aaaatgtcaa ggtgtgttga ttattgggca
     4561 ggtgctatct tctgtgattg aagaagtatc agcaattaat tcgaatgtcg tagtgattga
     4621 tgaccctagt attgaagctg aagtagatgc agtttatact gatttaacaa aagcaacgaa
     4681 tcagcattta gatcgattgt ataaaaaagg acaccgaaac attgcgttta tcggaggagg
     4741 acgtgttcgt ttagatagcc aaggaaataa acacgtgtca gatgatgatt atcgaaaaat
     4801 agcctatgag acgtggatga aacaaaaagg attagaaaaa tatagtcaaa tttatttaga
     4861 aggctggacc acacttgacg gaatgcaggc agcagattta cttttgaaaa aacatgctaa
     4921 agataagttg ccgactgcta tcatggtagg aaatgatcct attgctgtgg gggtttatcg
     4981 ttcacttcaa aaaaatggtc tacatattcc agaagatatt tcgattgtga gttttgataa
     5041 tattgaagtt gctgaatttt taacacctgc attaaatacg gtgaatgtga atacagaaga
     5101 aattggaaaa ctagcagttc gcatggttaa tgaacgaata aaagaagtta gaaatgtttc
     5161 gatcaaagtg attgtttcta ataatattat tgttagagaa agtgaaaaaa atatcgatta
     5221 aaaaagtaaa catccaaaaa agggtgttta cttttttgtt tactaatatt tttagtaaaa
     5281 aatctcgaca aaacattaag taaacagtta tggtgttgtt aacaaaaaga aaacgcattc
     5341 aaaacaggag gaattaagat gaaacatttc aaaaaaatag ctttaacagg gatagctgtt
     5401 ttaactggga cattactcgc agcatgtggt gggggagata gtaattctaa agacgggcaa
     5461 gtaactttaa gctatgcgag ttgagatact gaccaagctc caggactaag aaaaatgtta
     5521 gatgaatttg aaaaacaaaa tcctgatatc aaagtagaaa tggagacaac gccttgggat
     5581 caatattggg ttaaattaga agctgcaaca acgggaggta atatgccaga tgtagtaacc
     5641 atgcactcta gcgaatccta taaatatatg tcacagaatg ctttgatggg acttgatgat
     5701 gttatcaaag aaaatgatat tgacatggga aactttactg aaggaattgc tgatttctat
     5761 acgcttgaag acgaactata cgcaatccct aaagatgctt ctgtagtggg gctatggtac
     5821 aacaaagaat tatttgataa tgcaggtgtc gattatccag atgaaacatg gacatgggaa
     5881 acactcagac aagctgcaat tgatttaaca gatgaatcaa aaggcatcta tggttttgca
     5941 tcacatattg gaacagagac tggctactgg ccatttattt atcaaaatgg gggagaagta
     6001 tttgccgatg acaatacaaa atcaggtttg aataccccag aagcacaaga agctttagct
     6061 ttttatcgag atattgcact tgttgatcat gcttcaccaa caattacaga attacaagat
     6121 cctgataagt taagtcgttt tcaatcaggt agattagcaa tgatgattga aggaaactgg
     6181 tttgcaccgg gatttttgaa aaatgactat ctagttgaaa atgttgcttc tgccgtgtta
     6241 cctaagggaa aagttaatgc aacaattaca aatggacttg gttgggctgc cagtgcgagt
     6301 acaaaatacc cagaagaagt gaaaaaacta atggcatttt tagctagtga ggacgccaat
     6361 aaaatccaag cggaaacggg ggcatcaatc ccagcactaa aaggctttga agacgagtgg
     6421 gctgcttctt cagatacaat tgatttgagt gcttttggaa aaatgttgga gtatggagtc
     6481 cctcgtccat acaataaaga tggattacga gctgaacaag cagaagttca aattattaac
     6541 cgtattctgt ctggtgaagt aactgttgaa gatggaaccg ctcaaattga aaaagaagtc
     6601 aatgcgatat tgagcggaaa ttaaattagt taattataca atatacaggc gaaaggattc
     6661 tatttcgtct gtatattaat ggatgaatac caaaaagggg gatattatga aaaatcgaat
     6721 ttttggaaaa tatcgtaaag atcaagtgat atggggctat atttttattt ttccaacggc
     6781 attaggatta attttattaa atatatggcc agcaattcaa acagcaattt taagttttca
     6841 aaaaacggta gggttcggtg atactgaatg ggtcggatta agaaattacc aaaaaatgtt
     6901 tgcagacggt gaagtttttc agtctttatt aaacacatta atttatgcag tggtttcgat
     6961 acctagtatt gttgtattat ccttgttggt ggcagtttta atgaatagaa aagttaaagg
     7021 attgtcgatt tatcggacaa tttattttct accagtcgtt gctgcaccat ccgcagtcgc
     7081 aatgatttgg cgctggatgt tcaataatga ttatggactg attaataaca tgttggctaa
     7141 aataggtctc gatggaccag catggttaac ggatccatcg attgcgattt attctattat
     7201 cattgtagga gtttggagtg ctattggtta taacatggtg ttattactag ccggcttata
     7261 agaaattcct aaagattatt atgaagcagc ggaaattgat ggtgctagtc caatccagca
     7321 gtttttcaac attacgttac cattagttac accaacatta ttttttgtag ttgtaacaac
     7381 agttatcaat gctttccaag tatttgatgt tatttttatg atgattactc ctaatagtac
     7441 cgccatgttt aaatcacaat cattagccta tttattctat aaacattcat ttattttaaa
     7501 tgacaaggga tatggttcgg caatcgttat gttcctttta gtgattatat taattattac
     7561 tgcaattcaa atgaagttgc agaaaaagtg ggtaaactat taaggaggct atcaaattat
     7621 gaaaaataaa tttttttcaa atgcgccaat ccatatcatg cttattttag gtggtattgc
     7681 aatggcatta ccttttattt ggatgatttt aacttcaggg aaaacgttgg ttgaatctac
     7741 ccaaattcca cctacaattt tccctgagaa atttcagcta agtaactatc aacaagtact
     7801 tgatttatta ccattcatgg ctttttatat caatacattt ttaatggttg ttggacgagt
     7861 gattggctcg gttttatttt cagcaatggc tgcatatgct tgtgcgcgtc taagttttcc
     7921 agggaaaaat atccttttca gcttagtact tattcaaatg atgattcccg gttccatttt
     7981 tattactcca cagtatttat tagcacaaaa attgggtatt ttaaatacaa ttccagcatt
     8041 aatattacca ggggtagtta gtgcgtttgg tacgttttta ttaagacaat ttttcttagg
     8101 tttacctgat gaattggagg aagcggccta tttagaaggt gccaatattt ggacgatctt
     8161 tttcaaaatt atgttgccgc ttgctaaatc aggattggtt gcacttggaa tattcacagc
     8221 gttgtttgca tttaaagatt taatgtggcc attgattgtc aatacttcaa ttgataagat
     8281 gcctttagct ccgggattag caaacttaca aggacaatat gccacgaact tcccacaatt
     8341 aatggcaggt tcgttattag caatttggcc aatagttgtg atattcatcg ttttccaaag
     8401 aaaattcatt gaaggaattg ctacaacagg tagtaaatta taaaactatt aattagtgat
     8461 tggagagtaa gtacatgaca aaaattacat ttattggtgc aggaagtact atttttgcaa
     8521 aaaacgtatt aggcgatgcg atgttaactc caagtctaca agatgcacat attgcattgt
     8581 acgatattga tgaaaaacga ctaaaagaat caaatttaat gcttaaaaat attaataaaa
     8641 attcgaatga aaatcgtgct gatattgttt cttattatga tcgcaaagaa gcgttaagag
     8701 aggctgattt tgttatcaat gctattcaag tgggtggtta taaaccttca acagtgattg
     8761 attttgatat tcctaaaaaa tatgggttgc gacaaactat tggtgacacc atggggattg
     8821 gtgggatttt tagaacccta cggacactac cagtaatgtt agactttgct aaagaaatgg
     8881 aagaagtttg tccaaatgct tggttattga actatacaaa tccgatggcg atgttaacca
     8941 tgggtgtttt aaaagcgacg agtattaaga ctgttggttt atgtcatagc gttcaagtat
     9001 gcgtaccaga attgtttgag catttaggaa tagccgatca atatgactta aatgactttc
     9061 aatggaaaat tgcaggcatt aatcatatgg cattcttgtt ggaaattaca aaaaatgggg
     9121 aagatttcta tccaacaatt cgtcagttag ctgcagaaaa accaaatcca catccagatt
     9181 ctgtacgttt tgaattgttg aaacgttttg gttactatat tacagaatca agtgaacaca
     9241 atgcagaata ccatccatac tttattaaat caaaataccc agaattgatt gacgaattag
     9301 ggattccgtt ggatgaatac ttacgtcgtt gtgaacaaca aattaatgat tgggaaagca
     9361 tgcgagagga cattgtaaat aatggggagt taacacatac gagaagccgt gaatatgctt
     9421 catatatcat ggattctatt acgacaggaa ctccgtcagt gattgcagga aatgtcttaa
     9481 ataaaggctt aatcacaaac ttaccgacag atgtttgtgt ggaagtacct tgtttagtcg
     9541 ataaaaatgg cgtgcaacca acctatgtcg gtgaattgcc aacacaatta gctgctttaa
     9601 atcggacgaa tatcaatgtt caagaactga cagtagaagc cgctttaaca ctagataaat
     9661 caaaaattta tcaagcagcg tatttagatc cacatctatc atctgaattg tctttaacag
     9721 atatcaaaaa tttagtggat gatttaatcg aagcacatgg ggactattta cctgaatata
     9781 attaaaaaga agctgaaaga gtaaggaatt ttcaattcct tactcttttc ttagttaaat
     9841 ttagtaaaaa tattgacaaa acacttagta aacagttaca ttagtgttaa caaaaagaaa
     9901 acgcattcaa attgtataag gaggagcata aaatgaagtg gtctaaaaaa attattttag
     9961 cttgtatagc actaggagca acgatctctt tgactgcttg tggaaaaaat caggaagatg
    10021 caagcggtaa ggtagaaata gagtttttta atcaaaaaaa agaaatgact caaacgattc
    10081 aggagattgc taaagatttt gaagcaaaaa accctgatat tcatgtaaaa gttgtggatg
    10141 taccgaatgc tggtgaagta attaaaacac gtatgcttgc aggcgatgta ccagatgtca
    10201 ttaatcttta tcctcaaagt atagaattga aagaatgggc gaaagctggt tatttagagg
    10261 acttaaccga agaaccgtat ttagagaata tcaaaaatgg atatgcacaa cgttttgcta
    10321 ttgaggatcg tgtatatagc ataccattaa ccgctaatgt ctatggtttt tactataaca
    10381 aaactgcttt tgaggaaatg gggattcaag caccagaaac ttgggcagaa tttgaaaaaa
    10441 tagtagctga gattaaagat caaaacaaag taccgtttgc gattgcagga gcagaaggct
    10501 ggactttaaa tggttatcac caactagcta tagcgactgc tgctggtggt gaagaggaag
    10561 cgaacaatgt ttggcgtttc tcagaagtta atggaattaa tgctgaaagc aaagaaatgc
    10621 aaaaagattt taatcgatta gatttgttaa gagaaccaaa agctttgcaa aacaactggc
    10681 aaggtgccgg ttataacgat acagtagtta cttttacaaa aggtgatgcg ttgattatgc
    10741 cgaatggttc atgggcaatg ccgatgattc attcacaaaa tccagatttt gaagtaggaa
    10801 cttttccttt tccagcagat gaagcaggtc aaagtctaac gattggcgca ggagacctag
    10861 ccttatcagt ttctgcaact tctcaacata aagaagaagc caaacgtttt gtagaatata
    10921 tgacaacacc tgaagcgatg cagaaatatt atgatgtaga tggtgcaccg tgtgctgttg
    10981 agggcgttat cgagaatact caggattcac cattaagtgg tttaactgaa cttgctttta
    11041 ctgatcgtca tttagtttgg ttagccaaag attggaatag tgaaaatgat ttttatacct
    11101 taacaacaaa ttatttacac aatggcaatc aaaaagccat gattaacgca ttaaatgctt
    11161 tctttaatcc aatgaaagct gacggaaggt aggaaaataa aatgaaaata aaagaaattt
    11221 taaatcgtta ctgggcaatc atttttgtag caattccaat tattttgcag atgatttttt
    11281 tctactttcc aatggttcaa ggtgcttttt acagtttaac taattggact ggtctgactt
    11341 ataattttga ttttgtgggt ttgaacaatt ataagattct tttttctgat ccgaagttta
    11401 tcaaatcgat tggcttcact ttaattgtga cgctttgcat gattgctgga caaattggtt
    11461 ttggtatttt gattgcgcgg gcgttgaacg caaatattaa agggaaaaca ttctttagag
    11521 catggttctt ttttccagcg gtcttatcgg gcgtggttgt ttctttagta tttaaacaaa
    11581 tttttaacta tggtttacca aaaattggta gtatgttagg cattgaattt ttacaaacca
    11641 gtcttttagg aacaacatgg ggagcgattt ttgcagtaat ttttgtttta ctttggcaag
    11701 gaattgccat gccagtcatc attttcttag cgggttttca aagtattcca caagaaatta
    11761 ttgaagctgc tagaatggat ggggcgaatg ataagcaaat tttttggaat attgaaatca
    11821 cctacttatt accaagtatt tcaatggtat ttattatggc attaaaaggt ggcttaacag
    11881 catttgatca aatttttgct ttaactggtg gtggaccaag cgatgccaca acatctttgg
    11941 ggttacttgt ttataattat gcttttaaaa acaatcagtt tggttatgcg aatgctattg
    12001 ctttagtctt atttatctta attgggattg tttcactggc acaattaaaa ttatcgaaaa
    12061 aatttgaaat ctaggaggtt tcccaatgaa aacatcaaaa gaaaaaaata tttctaaata
    12121 cttgctttta ttattagggg gcttcatgat tttagttccc ttagtttcaa cgctattcgg
    12181 ttcatttaaa acaacacccg atattatgaa taatttcttt cgttttccta atccagcgac
    12241 tttggaaaat ttccgaagat tgttagcgga tggaattggt cattactttt ggaattcggc
    12301 aatcatcaca atcattgcag tagttgctgt gacagcaatt attcctatgg ctgcttattc
    12361 actagcaaga aaaatgactc gtgggaaagc atacggattg atgtatttat ttttaatttt
    12421 aggaattttt gttcctttcc aagtaattat gattccgatt accgttatga tgagtaaaat
    12481 tggtttagca aatatgtggg gattaatctt actctatctc acttatgccg taccacagac
    12541 actattttta tatgttggtt ttattaaact aaatgtgcct atcagtctgg atgaggctgc
    12601 tgaaattgat ggggctagtc actttacaac ttatttcaaa attgttttcc caatgttaaa
    12661 accaatgcat gcgacgacta ttattcttaa tgctctatgg ttttggaatg atttcatgct
    12721 accattatta attttaaatc gtgattctaa tatgtggaca ctaccattgt ttcaatataa
    12781 ttatacggga caatatttta acgactatgg tccaagtttt gcttcctata cagtaggaat
    12841 tgtcacgatt acactagttt atcttgcttt ccaaagaagt attatagctg gaatgagcaa
    12901 tggagcagtg aaataaccaa taaattattc tcattataaa ggagttcata aacttatgat
    12961 tatatttgac aatcacacac aaacatttca tttacaaaat aaaaaaatta gctatctatt
    13021 aggaatcgaa gaaaataatt atttaacgca tctttatttt ggaaaaaaga ttacgcacta
    13081 ttctggtggg tatcgctacc cccgaacaga tcgttccttc tcacccaaca atgcaaatgc
    13141 gcaagatcgt ttattatctt tagacacatt accattagaa tttccaggat atggacatgg
    13201 tgattttaga gaaccggcgc ataatattaa attaggaaac ggtagtcgtg taaatgattt
    13261 tcgttatgat agctacgaaa ttcaaaaagg aaagcccagc ttagcgggat tgccagcctt
    13321 atatacggaa gatgatgatg aagctgaaac gctgattatt caattgaaag acgcgattag
    13381 tggattgact ttacagttgt tctacacaat ttatcaaaat cacagtgtaa ttattcgttc
    13441 tacgcaatta gtaaataata gtgatgaaac agtggagatt aaccgcttag ctagtcaatc
    13501 aattgatttt ccaaatcgtc cactcgagtt aattcattta aatggtacct gggcaagaga
    13561 acgtcaaatg actagagaaa aaattcatac agggataaaa gtattagata gtaaacgcgg
    13621 atcaagcagc catcaacaaa atccgtttgt tgctttagta gatgccaaaa cagatgaatt
    13681 tcaaggagag gcatatggat ttaatttagt ttatagcggt aatcacgaaa cggtgattca
    13741 aaaagatcca tttgaccaaa cacgggtcat tatgggaatt aattcattta attttggttg
    13801 gcaactaaaa actggcgact catttcaaac gccagaagtc gtgatggttt atagtaatga
    13861 aggtctgaat acgttatcac agacgtatca tgaattgtat aatcaccgtt taattcgtgg
    13921 aaactatcgt ttaaaagaac gtcccacgtt aatcaacaac tgggaagcaa cgtattttga
    13981 ttttaacgaa gaaaaaatca tgaatattgt cgatgatgcg aagaacttgg gcattgaaat
    14041 gtttgtttta gatgatggtt ggtttggcgg tcgtgaaaat gacttcactt cattaggtga
    14101 ttgggttgaa acagaaggca aacttgcttg tggtttggaa aatttagcca agcaagtgca
    14161 tgataacggg atgaaatttg ggatttggtt tgaaccggaa atgatttctg aagatagtga
    14221 actatttcga gcacatccag attgggcatt gggtattcca ggtcgtgacc gctcaattag
    14281 tcgaaatcaa tatgttcttg atttttcaag acaagatgtt cgtgataata tctatcaaca
    14341 aatgacggcg atttttgatc gtgtaccgat tgattatgtg aagtgggata tgaatcgaaa
    14401 tatgacagaa gtctattctg tcttgttgga ctcaacgatg caaggtgaag tggctcatcg
    14461 ttatatgtta gggttatatg aatttttaga aaagttaaca acgaattatc caactatttt
    14521 atttgaaagt tgttcgggtg gaggcggtcg ttttgatgca ggtttcttgt attacatgcc
    14581 acaaacttgg acgagcgaca ataccgatgc cgtggctcgt ctgaaaattc aatatggaac
    14641 cagcttagtg tatccaattt caagtatggg tgcgcatgtt tcagcagtcc caaatcatca
    14701 aacaggaaga gaaacgagtt taactattcg tggcaatgct gcgatggctg gtgtttttgg
    14761 ttatgagcta gatctaacgc aattaacttc tgaagaaaaa gcagaaatta aagaacaagt
    14821 tgccttttat aaaaagcatc gccaactttt gcaatatgga actttttatc gcttggaaag
    14881 tccgtttgaa ggcaatgata ctgcatggat gtttgtctca aaagaccaaa cagaagccat
    14941 ggtcttttat ttccgagtat tagcagaagc tgcgcatcca ttagtcacat tgaaattagc
    15001 aggattagca gaagaagcaa cttatcaaat ggaagatgta acatttgctg gcaatgaatt
    15061 gatgaatctc ggtttttata ttgactcaga actacatggt gattatgcga cacaacgctt
    15121 ttatttgaaa cagatatagt agcttgaatt tacaatataa gtgcaacacc aaaagagact
    15181 cattccaaac gaagaaaata tgttttattt acacgcattt actaaaaagc agcctgattt
    15241 gaattgggaa aatgaagaag tacgtgaaga aatctatgca atgattaact attggttaga
    15301 taaaggacta ggcggattta gaatcgatgc gatattaaac atcaaaaaaa aactagagta
    15361 tggcatcttt gaatctgatg gagaagatgg gttagccttc attgggccct ggattcttaa
    15421 tcagtcgggc attgaagtgt ggttaaaaga aatgagagag cggacattta aaccacataa
    15481 tagtatgacg gtagcagaag ccgatgttcc taatgaacgt ttagatgaat atattggtga
    15541 agatggctat tattcgatgg tttttgattt tagttatacg gatattgatg tacctgaaac
    15601 tggtgaatgg tttaaagata gccaatggac atggacggat atgcgtacca atatttttac
    15661 gaatcaatta gttacgcaag ataaaggttg gggcgcctta tatttagaaa accacgatca
    15721 accacgttca attaataaat atattccaga agaatggatc aatgattata gtaaaaaaat
    15781 gttggcgacg ctttttatgt tactaagagg tacccctttt atttatcaag gacaagaact
    15841 aggaatgacc aacatagaaa tggcatcatt agaagatttt gatgatgtag ctacgcattc
    15901 tcagtataaa cgggcattag aatatggatt ggcaccagag caagcattga aagctgtcaa
    15961 taaacgaagt cgggataatt ctcgcacacc tatgcaatgg aatacgcaaa aaaatgccag
    16021 attctcaact tctgataatg tatggttaaa agtaaatcct aattatccag aactcaatgc
    16081 agaagctcag ttaaccaatc aacagtcggt attcaattat tatcatcaat taattaaatt
    16141 aagaaaaaat agtgtataca aagacgtgct aatctacgga caatttctac cagtgaaaga
    16201 taaaaatgaa catattttgc tttatgaacg ccaattagaa gataaaatcg tattagttat
    16261 cttaaacgtt acagcaacag aacaagaata ttatgtagat gaacagtacc aacaagtatt
    16321 aatcaataat tatgaagatg ttttattaga tcaaagtcga aaattgcgtt tacgaccgtt
    16381 tgaaagtatt gtattagcta attatggagg acaattatga aaattaaaaa cgaagcaatg
    16441 ctcatcacct atgcagatag tttaggaaaa aacatgggcg aattaaaaca agtcttagac
    16501 aaacatttac aaggtgttat tggtggtgta catctgttgc ctttttttcc atcgacaggt
    16561 gatcgaggct ttgcgccgag tgattatacg acagttgatc cagctcttgg aacatgggaa
    16621 gatgtggaag cattaggtga agattattat ttgatgtttg attttatgat taatcacatt
    16681 tctcgtgaat caaaattttt ccaagatttc aaaaaaaacc atgagcagtc accatacaaa
    16741 gaaatgttta tccgtattca tgaatttttc ccagaaaatc gaccaacaca agaagatatt
    16801 gatttaatct acaagcgtaa agacaaagcg ccgtttcaaa cggtagaatt tgcggatggt
    16861 acgacagaag aggtttggaa tacctttggg gaagaacaaa ttgatttaga tgtgaccaaa
    16921 gaagttgtga aagaatttat tcgagaaacc atcaaagata tggcgaacca tggctgttct
    16981 ttaattcgat tagatgcgtt tgcttatgcg attaaaaaat tagataccaa tgatttcttc
    17041 gtggaaccag atatttggga acttttagac gaagtgcgtc aagaagcagc gaaatatgac
    17101 gtcgaattat taccagaaat ccatgagcat tattccattc aaatgaaaat tgccaatcat
    17161 gattattata tctatgactt cgccttgcca atggtcactc tgtattcact ttatagtgga
    17221 aaatcagaac gtttagccaa ttggttgaaa atgagtccga tgaagcagtt tacaacattg
    17281 gatacgcatg atggaattgg tgttgtggac gctcgtgatt tattaacaga tgaagaattg
    17341 gattacacct ctgaggaatt gtataaagtc ggggcgaatg tgaagaaggt ctattcaagt
    17401 gccaattaca ataatttaga tatttatcaa attaacagca cctattatag tgcattaggc
    17461 gataatgacc gtagctatct gttagcacgt gcgattcaag tatttgcgcc agggattcca
    17521 caaatttatt acgttgggtt attagcagga aaaaatgata ttgaactgtt ggaagcaacg
    17581 aaagaagggc gtaatattaa tcgtcattac tatgatttag ccgaaattga agaagaagtg
    17641 caacggccag tgattcaaca attatttgca cttttaaaat ttagaaatga atctgcggcg
    17701 tttgatttgg atggaacgat tgaagtagca acgccatcag aatcagaaat agtgattaca
    17761 cgtaaaaata aaaacggtag tgatagtgca acattaacgg ctaacttgga tacgaaagaa
    17821 tttatcattg agtctaaaga tataaaaatt ttcactagtg ttgcattaaa attttaacct
    17881 attccataga acaaaaaatc ctttatacta gcacttggat gacttatcca agaccaataa
    17941 aaaggactca tacatggata agtataaaac aaatacagca tttaataaat ggttttctgc
    18001 cataaaatta ggaaaacttt ctgtcaactc ccaaaaatgt atttttgatt acaattcgta
    18061 tcgtaaaaaa ctgtcttttg aagctgtgct caagctcttt ctctacgcag ttaatgaaga
    18121 aaaagaaagt ttgcgtgatt tgagtacagc tcttatcgac cgatcgctcc aactagaaac
    18181 cgatgtgtca tcgattagtc atacccaatt gtctcgagca tttaatgcac ttgaacctaa
    18241 agtgttagaa gaaatttttc aacaactgtt agaaaaagtt caacatcaaa tcaaaccaac
    18301 caagcgaaat catctgtatc tgattgattc cagtacgttt tcgcttagtt taaaacgtca
    18361 taagtgggcg actttccgtc aaacaaaatc aggtgtcaag ttacacttaa actattgtta
    18421 tatggacgat actacgatgt atccaactga ttttacctta acaaatgccc atgaacatga
    18481 tctcaatcaa ctaccagtat tggtgaatca accagaggca acttacgtgt ttgatcgtgg
    18541 gtattaggac tttgaaaaga tggatcagat gcattgggat ggctatttct tcgtcactcg
    18601 gattaaaaaa aacaccaaag ttcacgtgat agatactctg gaaacatcgc cagaaacaga
    18661 aattctacga gacgagctgg ttcgtttagg atctaaaacc tatctaaccg ccaatttccg
    18721 actagtcact gttcaagata agaacggacg tgtgtttcaa tttatcacca atcgaatgga
    18781 cgtctcttca aaagaaatct ctgatatgta tcacgctcgt tggcaaatcg aattgttttt
    18841 caagcacatc aaacaacaca tgaccattaa aaccttcttt tctcaaagtg aaaaaggcgt
    18901 gcagaatcaa ttgattttaa cgatgatttc cgctctcctc acgtttctca tcaaattgga
    18961 gacgaaaacg gaaaaatcag tgttccaaat caaacgattc tttcgttatt tactgtttca
    19021 gccctttgaa tgttgtctag aaaaattaat tcccacatga gaaaaaagaa atttggctcg
    19081 ttacttttgg ttttcagttg aataacagat aaaaaatttt ctggaaaaag tcatcctctg
    19141 cgtcagcaca gttttttttg acttattttc agaggaaata gttttcagaa tattctaaaa
    19201 tgttttatgc aacactagtg atatttttta gaattagatt tctaatgtgc attttttgag
    19261 tattaaggag caagtataca aagatttata aagctgatta aatttgaata aaaatatgca
    19321 ggaacgctac tatttttagt agtgttcttt tttatatttg aataaaaaat gtggtggtta
    19381 aatggtggtt ataacaaaaa atgaattaat gaaattaggt tatgggaagt atcagtctga
    19441 gagaatcata cgacaggcaa aagcaattat ggtttcaaaa agctatacct attacgaaaa
    19501 tagaagatta ggactagtac ctgttgaatc tgtagaagaa gttttaggta tgaagataga
    19561 ggtagaggag gattttcaga atcatggtta attttaaaaa agataaaaat gggaaatatc
    19621 attttactgc tagtttggga ttcgacaaac gaacagggaa aagagtacaa aaaaacgtag
    19681 aggggttaaa acagttaaag aagaaaaaaa gcgtataccg aacttgtttc taattatgga
    19741 aaagaagcag aaacttctca aagtactatg ttgtttgagg agttttttta taaattattc
    19801 ctaccttatt atcaagggaa agtagaagaa agaacatata aaaatcggtt atctacaatc
    19861 aaaaaaactt ttcttatttc tttaaaatgg agttaagaga aatacgtcca attgacattc
    19921 aaaagtggca aaataattta ttgagcaaat ataaaaattc ttatgtaaga aacatgtatg
    19981 ggatttttca gatgtcatta gatcgagcag ttgttttggg actacttgaa aaaaatcctg
    20041 caaaagttat tggaaatgtg aagaaaagga aaactacagt tgatttttgg acaaaagaag
    20101 aatttacaaa agttatttca actttttata ttgatgattt gtaccaacat tttggtttta
    20161 tttgtatgtg gttacttttt atgaccgtta tgcgtttggg agaagccact gcattaactt
    20221 ggaaagatat tgattttgaa aataaaaact tgcgtgtgaa taaaacactc tattataaga
    20281 gagccaaaac ctatgagttt gtagaacccg aaacggcttc tagtattcgt acaatcgaac
    20341 tagacgacaa tacaattacc tttttaaaag cttggaagga gcgtcaagtt tcaataggag
    20401 gcatgtcgtt tgtactttct tataatggaa cacctactca gaaatataca atccgtcata
    20461 ttatgagaag acatgcaacg atcgttggtg ttcatataat ccgcatacac ggattaaggc
    20521 atagtcatgc ttctttgctt atatcacaat aaaaagatgc acttcgaatt aaagaaaggt
    20581 taaaacatgc tgatgtacaa acgactttag gaacttatgg tcatttgtat ctaaattcat
    20641 tgagagagat aacaaatgat cccacagatt atatccaaac taatttttct aaaatcaaac
    20701 cgacacctat ttacgataat aaaaaataaa tgataaacaa aagtaggatc acaccaaaag
    20761 aaacacacta gaaaaactat aatacaggct tttctagcgt gttgtttctt atttccgcct
    20821 atttatacat tatactcata aagcatttaa tgcatcgatc gtagccgcat tgtctatatg
    20881 ggtatataaa gttgttgtat tggtacttga atgtcccaat tgttgggcag ttaataccaa
    20941 tgaatttgtc tgttggtaga gtctagtagc taatgtatga cgcagtttgt gagggctgac
    21001 ttgaattcta aagccttttg aatattttgc taccatccgt tcgattgcaa taccggatat
    21061 acgcttggct tctccttggt aaattgctag aaataaagct ttttcgtttt ctgttacttt
    21121 atatcgttct ttccgaatag ctagataatt agctaaatat tccattgcga atgctgctat
    21181 attgacagca tctcttttat ttccttttct agttactacc acattattct tatctaaaga
    21241 taggtcatct aaattaatat ttataacctc ggagagtctc aatccagatc ctaaaatcag
    21301 tgcattgatc gccaggtctc tttctacatc tcgttgataa tagatcaaag ctcgtttact
    21361 caaagtattt ttatattcat tggcaatata atctagataa ttttgtgtat ccttatctag
    21421 aaataatttt ttctcaatgg cattggcacg tgcagctaac gtagttttat ctgtgatggt
    21481 tgctacttga tccataggat tattgtctaa gtatggtttt cctagaggat tattggaact
    21541 tttcgaaaga tatttaaata gaacctttaa tgccgtgata tttctattga tagtagaagt
    21601 agatagacct aagttattat tatctttgag cttagttttt gttgtctctt tttttacatt
    21661 ttcttttgtg cgacgtttca aatgagattt gtacaagttt aggtcatagg cagttaactg
    21721 tcttatcgag gtgatagaag tatcaggaac gatagactca ctcagaagcc attcataaaa
    21781 gagcttataa ttttttagat attctaaagt ggttcgaggt gattgatctt tatcctcacg
    21841 atcttgaaca aaatctttta tataatcggg catatgaagt atgattgtat ctattttttg
    21901 atatatttct gtatttttca tgtcattcat cctttcaata agtaggggag aagagtatct
    21961 ctataattgg actataccaa ttatagaatg ttttgtcaat tggtatgctc taaaatttat
    22021 taatttgttt caaaaaaagc atataaacag gtatacgttt aataatttaa aataaatggt
    22081 atacataaat agggaaggaa atagaaggga gcaatctaaa ttttttgaat tttcttaatt
    22141 aattatatag aaagaacaat ctttctatag agttagacat gagagggact atgcttgatt
    22201 taaaatcaac aagcgtatat actgtatata caaagaaaaa ggagggagaa gactatggca
    22261 attaaagaaa gaaaatttag ggaagtgggg aactcagtag tattaactat ttcaaaggag
    22321 tttcttaaag aaaatgggtt aaatcccgga gatacggttt ttttagatga agaaaaactt
    22381 cgagcggcaa ttacaaaaaa acaagcctta tctgatgaaa ttgatttagc aatatctcaa
    22441 gcattaactg aatatgatac tacttttaga gagcttgtcg ataaatgagt attcattacc
    22501 tagaagccaa agatattata aaaatgaatg taatccaaat aaaaaaatac tctcctaatg
    22561 aaccccttgg tgtaaaggat gcgaatgctt tagaaatgtg tgtagggcaa cttcgagcct
    22621 cagcttttgg tgaagaggtt tatccttcga tttatgaaaa agcagctatc ctacttatac
    22681 aattgataaa gaaacatcct tttcacaatg caaataaacg tactgctttc ttagctactt
    22741 ttgtcttttt gaaaatcaat ggacagcttt tagctattga tcaaaaagat gctgtaaatt
    22801 tagcggtata tattgctacg tatgataagg atttcgatca actaaaatat gaagtgatca
    22861 acactataaa gacacatacg cagcccattt aaggagttac tgtatgtgtc tttatagggt
    22921 tattagctgc tatagcgaaa taattgaagt aaattacaaa tagctataat tcaaatccaa
    22981 ctatatagaa aacgtaaatt ttctatatac ttggatatgc tggaatgttt aagagaataa
    23041 ttacatttct agaaaataca ataaaaatta caggataatt agccatatcc tacctcctct
    23101 agcagttgct caaaagttag cactaagtaa cttctttttt gttgctggta acaatttatc
    23161 aatactaaaa tagcgtttaa gtatctaata aatactaatt ttaaagctct taattattat
    23221 gaaacacgat ataacaatat tgctatatcg tgctattgta atattataca tttgaagaca
    23281 gcgtattctt gttctaatat caggaatttt ttataaatat ca
//
LOCUS       DAZULO010000035        22832 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000035 DAZULO010000000
VERSION     DAZULO010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.036"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..370
                     /locus_tag="ACG8OA_001378"
     CDS             2..370
                     /locus_tag="ACG8OA_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFS4976171.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            550..1491
                     /locus_tag="ACG8OA_001379"
     CDS             550..1491
                     /locus_tag="ACG8OA_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFS4976172.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1560..2291
                     /locus_tag="ACG8OA_001380"
     CDS             1560..2291
                     /locus_tag="ACG8OA_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4976173.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2435..4615
                     /locus_tag="ACG8OA_001381"
     CDS             2435..4615
                     /locus_tag="ACG8OA_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFS4976174.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4635..5117
                     /locus_tag="ACG8OA_001382"
     CDS             4635..5117
                     /locus_tag="ACG8OA_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFS4976175.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5133..5894
                     /locus_tag="ACG8OA_001383"
     CDS             5133..5894
                     /locus_tag="ACG8OA_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS4976176.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6025..7359
                     /gene="murC"
                     /locus_tag="ACG8OA_001384"
     CDS             6025..7359
                     /gene="murC"
                     /locus_tag="ACG8OA_001384"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFS4976177.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7666..8151
                     /locus_tag="ACG8OA_001385"
     CDS             7666..8151
                     /locus_tag="ACG8OA_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFS4976178.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8144..8827
                     /locus_tag="ACG8OA_001386"
     CDS             8144..8827
                     /locus_tag="ACG8OA_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFS4976179.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8953..11598
                     /gene="polA"
                     /locus_tag="ACG8OA_001387"
     CDS             8953..11598
                     /gene="polA"
                     /locus_tag="ACG8OA_001387"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFS4976180.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11643..12479
                     /gene="mutM"
                     /locus_tag="ACG8OA_001388"
     CDS             11643..12479
                     /gene="mutM"
                     /locus_tag="ACG8OA_001388"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFS4976181.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12443..13072
                     /gene="coaE"
                     /locus_tag="ACG8OA_001389"
     CDS             12443..13072
                     /gene="coaE"
                     /locus_tag="ACG8OA_001389"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFS4976182.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13124..13456
                     /locus_tag="ACG8OA_001390"
     CDS             13124..13456
                     /locus_tag="ACG8OA_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS4976183.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13768..14259
                     /gene="nrdR"
                     /locus_tag="ACG8OA_001391"
     CDS             13768..14259
                     /gene="nrdR"
                     /locus_tag="ACG8OA_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFS4976184.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14282..15655
                     /locus_tag="ACG8OA_001392"
     CDS             14282..15655
                     /locus_tag="ACG8OA_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS4976185.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15655..16581
                     /gene="dnaI"
                     /locus_tag="ACG8OA_001393"
     CDS             15655..16581
                     /gene="dnaI"
                     /locus_tag="ACG8OA_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFS4976186.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16710..17150
                     /locus_tag="ACG8OA_001394"
     CDS             16710..17150
                     /locus_tag="ACG8OA_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS4976187.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17294..17995
                     /locus_tag="ACG8OA_001395"
     CDS             17294..17995
                     /locus_tag="ACG8OA_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFS4976188.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17992..19113
                     /locus_tag="ACG8OA_001396"
     CDS             17992..19113
                     /locus_tag="ACG8OA_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFS4976189.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19128..20360
                     /gene="pepT"
                     /locus_tag="ACG8OA_001397"
     CDS             19128..20360
                     /gene="pepT"
                     /locus_tag="ACG8OA_001397"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFS4976190.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20492..21400
                     /locus_tag="ACG8OA_001398"
     CDS             20492..21400
                     /locus_tag="ACG8OA_001398"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4976191.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21435..21707
                     /locus_tag="ACG8OA_001399"
     CDS             21435..21707
                     /locus_tag="ACG8OA_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFS4976192.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21718..22764
                     /locus_tag="ACG8OA_001400"
     CDS             21718..22764
                     /locus_tag="ACG8OA_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFS4976193.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         7697 a         3981 c         5012 g         6142 t
ORIGIN      
        1 gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa aaaatgcaac
       61 gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg ggacgcttat
      121 aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc gagcatttgt
      181 tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt acagtaaaga
      241 tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg atatggtaca
      301 gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact ttgcggttac
      361 aggtaaataa tagaaataaa gactttgttt ttctgtgcga aacaggaaag cgaagtcttt
      421 ttaattcaaa taataatatt atgtaaacaa aaaactttcc atgcgtaatt atacacaagc
      481 aaataaatcg tcaaatgcga acgccttctt tatactgttt aggaacgaaa agaaggaagg
      541 aagaaataca tggattatca agtactgctt tattacaatt atacaaaaat cgaagaacca
      601 gaggaatttg ccaaaaatca tttagccttt tgcaaatctt tggatcttaa aggtcgtatt
      661 ctagtagctg aagaaggaat aaatggtact gtgtctggaa ccgttgaaaa cacgactgcc
      721 tatatgaaag caatgaaagc tgatcctcga tttgctgata cgatgttcaa gatcgatcca
      781 agcgaaggac atgcctttaa gaagatgttt gtaagatacc gtccagagct tgtaagttta
      841 aagctagaag aggacattga tccaaaccaa gtgacaggtg cctatctttc accgcaagaa
      901 tttaaagaag ctattttaga tgaacagaca gtggtgattg atgcacggaa tgattatgaa
      961 tatgacttgg gacattttcg aggggctgta cgcccagata tacgcagttt tcgagagctt
     1021 ccagactgga tccgcgagca caaagaagaa tttatggaaa aacgggtagt gacctactgt
     1081 actggtggca ttcgttgcga gaagttttct ggttggcttg tccgtgaagg attcaaagat
     1141 gtcggacagc tgcatggcgg tattgctact tatggtaagg atcctgaagt ccaaggagaa
     1201 ttatgggacg gcaagatgta tgtgtttgat gaacggatcg ctgtcgatat caaccaagta
     1261 gataaacaaa tcgtcggtcg cgactggttt gacggaacac catgtgaacg atatatcaat
     1321 tgtagcaatc ctgagtgcaa tcgtcagatt cttgcttcca ttgaaaatga agaaaaacat
     1381 ttaggagcat gtagcataga gtgtgcgaaa catccgcgca atcgctatgt tcaagaccat
     1441 ggaatctctc cagaagaagc agaactgcgg tttacagctt taattaaata gaaaacaata
     1501 gttttgattg tcgaccattt ccaatatgcg ctatactagc gttgaggatg gtgatccaca
     1561 tgaaatcatt gatttatgct attggggatg tccatggcaa acatgagctt tttgaaaaac
     1621 ttattcacta ttatgatcca aagatccatc agcttgtttt attaggggat ctcaatgatc
     1681 gaggtcctcg tacaaaagaa tgttttttat taggaaagca attagtcgaa gaaacaggag
     1741 ccatttacct ccggggaaac catgaagaat attttttgca atttctgcaa tcaccggagg
     1801 actggtatgc gccttatatg agaaatggtg gaaaagaaac gtttgaaagc ctgattcacc
     1861 caggggcatc ggaggaatac tccccaactg aaatctcgat gatgatccaa tcgagatatt
     1921 cagaactgat taaattttta gtccagcgcc ccttgtatta tgaatgggaa aattatcttt
     1981 ttgtccatgc cggtgtggat ttgacaaaga aagactggcg taagacaagt ctaagagact
     2041 ttatttggat aagggaacca tttcatgaag ggaaaaacaa tacaggtaag acaattgtat
     2101 ttggacatac gattacacca atgctccatg gagatatgca gacaacagac ctctggattc
     2161 aggatcataa aatcggaatg gacggtgggg ctgtattcgg agggtcattg catggggttg
     2221 tttttgacaa ggaaggtatt cttcaagata ttgaatatca gaacaacgaa ggaccttggc
     2281 atggaatctg actaatggaa agaatgaagg agctgtgact ttgttgcggc tctttttatt
     2341 tttatagaga atattgtttc tttcagtagt ttaacccagg ataaaaagta tgatagaata
     2401 aagaagcaat ttttagcaga aaagaggcat tttaatgacg gaaaatttga accagacgat
     2461 cattcagctt gtacaaaaag aactttcaga ctatcgtcct aagcagttga ctactgtact
     2521 taatttgtta aatgaaggaa acactgttcc ttttatcgcg cgttaccgaa aagaaatgac
     2581 tggaagtctt gatgaagtcc agattcgtga aatcgaagag cggtatgcct atcttgaaaa
     2641 cttagaaaaa cgtaaaactg aagtgattcg gctaatcgat gaacaaggga aactgactcc
     2701 cgagttagaa gcagaaatca cccaagccgt gaaaatgcaa caagtggaag atttgtatcg
     2761 tccatacaaa caaaaacgac ggacaaaagc aacgattgct aaagaaaaag gacttgaacc
     2821 actcgcgaag tggctgatgc agcttacaga tggagaggtg caatctgaag cagaaaaata
     2881 tattgacaaa gaaaaagaag tctcttctgc cgaagaagcg ttgcacggtg ctcatgagat
     2941 tattgctgaa caagtcagcg ataatgcaaa atttcggact tggattcggt cttatacgta
     3001 taacaaaggt atgtatgtca gcaatgtcaa ggatgagcaa gcagatgaaa aaggcgtata
     3061 tgaaatgtat tatgactttg cagaacccgt ccataaaatg gtttctcatc gaatattagc
     3121 tactaatcgt ggagaaaaag aagatattct gaaagtattc tttcaagtag atgaagcagc
     3181 aattttagct tacttggatc gccaaatagt caaaaatcct gcctctcctt cttcttcatt
     3241 cgtcagagaa gcctatcagg atagttataa acgctttatt cagccagcaa tcgagcgaga
     3301 actgcgcaat gaattgactg aaaaggcaga tgagcaagca atcgctattt tcggcgaaaa
     3361 cctccgtaat cttttgttgc aaccgccact aaaaggtaaa gttgtcctgg gatttgatcc
     3421 ggcttatcgt actggatgta aactagcagt cgtagatgcg acagggaaag ttttagcaat
     3481 cgaagttatt tatcctcata aacctgcagc gcaagcaaaa agagaagctg ctggaccagc
     3541 ttttatccaa ttaattaata aatatcaagt agatatggta gctatcggaa atggtacagc
     3601 cagtcgggaa tctgaacttt ttgtggctga gcagttgaaa tccgccgacc ataaaaccta
     3661 ttacgctatc gtcaacgaag caggcgcttc tgtttactcc gctagcgaaa ttgcacgaaa
     3721 agaatttccg catctacaag ttgaagaaag aagtgctgtt tctattgccc gtcgcctgca
     3781 agatccttta gctgaattag tcaaaatcga tcctaaagct gtcggagtag gccagtacca
     3841 acatgacgtt tctcaaaaac gtttggctga acaattggat tttgttgtgg aaacagcggt
     3901 taaccaagta ggtgtggatg tgaatacagc aagcccgcag ctcttgcagc atatatcagg
     3961 attgaataaa acaactgccc aaaacattgt catttatcgt gaagaaaacg gagaattcac
     4021 cgcacgaact caattgaaaa aagtgccacg cttaggacca aaagcatatg aacaagcaat
     4081 cggattcttg cgagtccctg gtgggaaaaa cattttggac aacacaggta tccatcccga
     4141 aagttattct attgcaaaag aaatattaac ttcagtccaa ctttcagaaa aagagcttgg
     4201 tacagaagaa gcagtagaaa aattgacacg attatccgta gagaaactgg ctgaaagcct
     4261 atcagtcgga gaagaaacgt tagccgatat tttagctggg ctgacacaac ctggacgaga
     4321 tatgcgtgat gagatgccag cgcccttatt acgtacagat gtattgagta tggaagactt
     4381 gaaaccaggg atggaattga ctggaacggt ccgtaatgtc attgacttcg gcgcatttgt
     4441 tgatatcggt gtgaagcaag acggacttgt ccatatatcg aaactcagca aaaaattcgt
     4501 caagcatcct acagatgtcg tgtcagtagg agatatcgta acagtttgga tagaacaagt
     4561 cgatacgaaa aaaggcagaa tcagtttgac gatgctttcc ccttatgaag agtaacaatg
     4621 aggagtaaca aaaattgaca caagaagaac tgcagcagtt agtagaagag atatcattaa
     4681 aaagcttcca gcttcctttc aagcaccaag caatgttcaa tccacgctta cgtacaactg
     4741 gcgggcgcta tcatttaaac gatcatcatt tagattttaa tgcgaagcta tttgaagagt
     4801 cagacgaaga gacgattgca ggaatcatca aacatgaact ttgccattat catcttcatc
     4861 tagctggaag agggtatcag cataaagata tggatttcaa gtatcttttg aaaaaaacag
     4921 gagggttgcg ctatgcacca gccttgacga agaaacagat aaagatggag tattatcgat
     4981 gccaagattg ttctgccacg atctgccgca agagaagaat cgatgtacgc aaatatcgtt
     5041 gtggaagatg tcatggagaa atcaaatgga ttgagacaaa aagagaagac tttaatgaag
     5101 cacaaaaaat cagatgaaag gatagaaggc ctttggaaaa aagtgagcag aggaatctaa
     5161 aagactggaa tcagctatta aattttttag ccgcacctga agagggaaat tttgaggaac
     5221 tgacggcacc tacacttatt ttagctggaa actgtcttcc tatcttagct gataaagcag
     5281 cacaaatgta tttaaatggc caagtagatc aattgttttt ggtcggaggg gtggggcatg
     5341 cgactagaat cctttatgag aattttgaaa agcaaggatt tcattttgaa gatggcatga
     5401 gtgaaagtga aatctgtcgt cagtatctaa aagaagtcta tgatttgccc gataaggctt
     5461 ttctgataga atcgaagtct acaaattctg gagagaacgc cactttttca ctggagattc
     5521 ttcattcctt aggagctgta ccagaaaaaa tcttattgat gaatgatccg acactgcaac
     5581 gcaggactcg agcaactttt gagaaagtat ggcaaaatga acaaactatt ttcgcgaatg
     5641 ctgtgccatt tgtgccggaa atccttcact tttctgaaga gatcatcttt acagcaaaag
     5701 aactgaatca tcaatggcct aaggaatatt tccatgcgct agtattagga gaaatggaac
     5761 gtctccatga cgatgagaac ggctatggtc cgaaaggaaa agattttatt cctcacattg
     5821 atatatcaga ggaagtttgg tcttcttaca ccagaattaa agagtcaata aaaacggatt
     5881 tttctcgtac ttgaccattt tagcaagcga atcctccttt ccaaataaat ttggttgaaa
     5941 ttttcaatcg tttataagat aatgaggagc gtgttgaatg taatagctaa aagaatggac
     6001 gctagataag agaagaggat tcaaatggaa aatcaaaata aattgtatca ttttgttggt
     6061 atcaaaggtt caggaatgag ctccttggct cttgttttac atgaacaagg tctaaatgtc
     6121 caaggatcag acattgaaaa atatttcttt acacaaagag atttagaaaa agcaaatatc
     6181 acgattctgc catttaacgc agataatgta aaaccaggga tgacgatcat tgcaggaaat
     6241 gcatttcctg attcacatga agaaatccag cgagcaaaag aattaggttt ggaagtgatt
     6301 cgctatcacg actttattgg tcattttatc caaaattata cgagtatcgc tgtaacaggc
     6361 tctcacggaa aaacaagtac gactggactg ctctctcatg tattgagtgg tgtccgtcca
     6421 acaagttatc taattggaga tggaacagga catggtgatc cgcaagcgga attcttttca
     6481 tttgaggcct gtgaatatcg ccgtcatttc cttgcctatt caccagacta tgcaatcatg
     6541 accaatatcg attttgatca tccggattat tatacaagta tcgacgatgt atttacagct
     6601 ttccaaacaa tggctggaca agtgaaaaaa gctatttttg cgtatggtga cgatgcttat
     6661 ttacgcaaat taaaagcaaa tgtgccgatt tattattatg gtgtcacaga aaacgatgat
     6721 atccaagcac gaaacatcga acgcacaact tctggttctg cttttgatgt ctatcatggg
     6781 gatgaatttg ttgggcactt cactgtacca gcattcggta aacacaatat cttaaatgct
     6841 ttaggtgtta ttgcagtagc ttatttcgaa aaactggatc tgaaagaagt tgcagaagaa
     6901 atgctgactt tcccaggagt aaaacgtcga ttcagtgaaa agatcgtagc agacatgacc
     6961 gtagtcgatg attatgcgca tcaccctgca gagatcaaag caacgattga tggcgcacgc
     7021 caaaaatatc cagataaaga aataattgca gttttccaac cccatacgtt tacgcgtacg
     7081 attgcattga tggatgaatt tgcagaagca cttgatttag ctgataaagt ttacttatgc
     7141 gatatttttg gttcggctcg ggaagaacaa ggaaacgtta agattgaaga ccttggagcg
     7201 aaaatcaaaa aaggtggaga agtcatcaaa gaaaataatg tatctccatt gttagattat
     7261 catgatgcag tagtaatctt catgggtgct ggagatgttc aaaaatttga acaagcgtat
     7321 gaaaaacttt taagcagcac gacgaaaaat gtactgtaat acaattactg cagtgtcctt
     7381 ataaaaatag aaagaggggg agtggcaaaa atcttgccaa gccccctttt ttgcaactta
     7441 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaatcaaa tcaaaaacat
     7501 gagaagctgt tttttcttgg ctgttcttat tatttgatgc aggacagctt tttcacgacc
     7561 tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt acgctacttt
     7621 tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa cataggtaag
     7681 ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt atccttaaca
     7741 gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga tgcaaatcct
     7801 ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat cgttccatca
     7861 gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg accaggatca
     7921 catgttgtca atttttctgt caattttatt gaaccggttt tccattatga aacactgact
     7981 tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat ttctgtagaa
     8041 gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca accgccacaa
     8101 gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata atccaagtac
     8161 atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag gtatgggact
     8221 tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc aattagcaac
     8281 ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac tgattttagt
     8341 aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg gatttattgc
     8401 ctactctctt ttgaatggca taatattagc ggttactctt gcatactacg atattggaac
     8461 agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg ttgtcggagt
     8521 aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt taatcggagt
     8581 gatcattgca ctagtcttga acatcttcat tttgagaagt agcgtagttg agctgttcat
     8641 ctccctactg ttagtcgtca tctttgcagg gattacagct tatgataacc aaagcatccg
     8701 taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat tcatggcatt
     8761 gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct ttggacggaa
     8821 caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg gtatggtccc
     8881 gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac tttacagtaa
     8941 ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag tgtggctttt
     9001 cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa cggtcttcat
     9061 acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa agagcaaccg
     9121 actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga aatgtatgcg
     9181 gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat gccatatata
     9241 cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta tgaagcagat
     9301 gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt cgtcgtgctt
     9361 tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga tatcactgta
     9421 aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa atatgatggg
     9481 ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc tgacaatatc
     9541 cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca atatggctca
     9601 gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa agaaaatctg
     9661 ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa tacttcagcg
     9721 cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga taaactcgtt
     9781 ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat cgtagaagaa
     9841 gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga ggaaatgttt
     9901 actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac agaggaaatc
     9961 gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct atctgtattc
    10021 gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa agtatatgat
    10081 gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg aatcgatttt
    10141 gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga tatcgaagga
    10201 atagctgctc attacggata caacgatatc caatcagatg aagctgtata cggcaaaggg
    10261 gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc gcgaaaagta
    10321 gctgcaatca atgcgttgac accaaaattg agccaagaac tggcagaaaa aaaccaagaa
    10381 gatcttttcc ggaaaatgga actgccattg tctatcattt tggcagagat ggaaataagc
    10441 gggatcaaag tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc tgctcgctta
    10501 agagagatcg aacaaaaaat ctacgaagaa gccggcgaag aattcaattt gaattcacca
    10561 aagcaattag gagtgatctt atttgaaaaa atgggattac ccgttatcaa aaaaacaaaa
    10621 actggatatt ccacagccgt agatgtactg gaacagcttc gtgaacaagc accaatcgta
    10681 gaggatatcc tcacctatcg tcaaattgca aaaattcaat ctacctatgt cgagggactg
    10741 ctgaaagtga tccaatcaga tggaaaagtg catacccgtt atgtccagac attgacccaa
    10801 acaggacgat taagctctgt tgatcctaat ttgcagaaca tcccgattcg attagatgaa
    10861 gggagaaaga tacgtcaggc atttgttcct agagaaaaag actggctgat ttattcttct
    10921 gactattcac aaatcgagtt acgcgtactt gcgcatatct caaatgatga acatttgaaa
    10981 gctgccttcc tagaaggtca agatatccat gccagcacag cgatgcgtgt atttggaatc
    11041 gaaaaggcag aggatgtcac accaaatatg cgtaggcagg caaaagcagt gaattttggt
    11101 atcgtgtatg gcatctctga ttacggactt tcacaaaatc taggaatcag ccgaaaagca
    11161 gcacagcaat acatcgatac gtactttgaa aaatacccag gtgtcaaaga atatatggaa
    11221 agaatcgtac gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca ccgccgtcga
    11281 tatctgccag acatcaattc aagaaattac aatatccgtt cttttgcgga acgaacagct
    11341 atcaatacac caatccaagg aagtgctgct gatattttga aaatcgcgat gatcgaattg
    11401 gataaacgat taaaagaaac aggtcttcaa gctacgatgc tgttacaagt tcatgatgaa
    11461 cttgtcttcg aggtaccgga aaaagaactt gaatcattag ataaattggt gaaggaagtt
    11521 atggaacaag ctgtttctct tcatgtacca ctgatcactg acagcagttg gggaaaaacg
    11581 tggtacgaag caaaataaac attgtttaag acagagcaat ataatgaagg tgaaaagaaa
    11641 aaatgccaga attaccagaa gtagaaaccg ttcgtaaagg attggaacgg ttagtagtag
    11701 gaaaaacgat tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa ccagagacac
    11761 ccatctttga ggcttctctt gttggcgaaa ccatccaatc cattggacgt agagggaagt
    11821 ttttaatttt tcatctcgat cattgtgaac tgatctcaca cttgcgaatg gaaggaaaat
    11881 atcaatttac gaaagaaaat acaccaatcg ataaacatac gcatgtactt ttcttttttg
    11941 aggatggaag ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg acaatcgtcg
    12001 aaaaaggagc aagtgcaact taccgaggaa tcatgaaatt gggacctgag cctttgccag
    12061 atagcttctt attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa gcaatcaagc
    12121 cattgctgct agaccaacgt ctagtgactg gtctaggtaa tatttatgta gatgaagcat
    12181 tatgggaagc gaagatccat ccagaacaac cggcgaacac actgcgcccc aaagaggtag
    12241 aacaattacg tctatcgatc attgatgttt tggatcgtgc aatagaagcc ggaggtacca
    12301 cgattcgcag ttatttgaat gcattaggag aatcaggcgg atttcaagta gcactgcatg
    12361 tctatcagca aactggcaaa ccatgtatac gttgtggaac gccgatcgtg aaaacaaaag
    12421 tggcacaaag ggggacgcac tattgtccga attgtcaaag gctaaaaatc gcaaagtagg
    12481 atttgtttta ggtttgacgg gtggaatagc taccggtaaa agtacggcag ccaaagtctt
    12541 tcagtctcat ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa ctgttgagcc
    12601 tggaacgctt gctctgagga aaattgtatc agtgtttggc caagaggtat tgcagcctga
    12661 tgggcaacta gatcgaggga aacttgggat gattgttttt ccatcaaagg aaaaacggca
    12721 aaaacttgat cagctattag atccgtttat ccgaaaagcg ataaaagatc aaatcgcctc
    12781 tctaagttcc aatcatcctt tagtaattgt agatatccca ttgctctacg aaggtcatta
    12841 tgaccactat atggatgcag tagctgtcgt ttatactacg ccagaaactc agcttcagcg
    12901 tttgatgaaa cgtaatcaac tgacgctaga acaagcgcag cagagaatta gtagtcaact
    12961 tccaatcgaa gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg gaacaaaaga
    13021 aatacttgtg ggacaaatcg aaaattggct ttttgctaat cgctttcttt gagtatctgt
    13081 gtcaattttg ctatcctgag tatatagaaa gtgagggata gcgatgggat cattaaaaaa
    13141 tcaaaaggat aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc tcaataaaga
    13201 aaatctaatc gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag aaaagcagta
    13261 ccaagcaaga atcgatgaaa tagaagacca gcaggcagaa gaagaagcga agcggcaaga
    13321 tttgactcaa gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc tcccagaaag
    13381 tgaccacatt cccgatgagc aaaaaatcga ggaacagcga atgaaacact atacaggaat
    13441 cgaagagaag ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga tgagaaggtg
    13501 ttcatctgct tcaagttttt tgtgctagat gactaaatca gcttttgtgt aagttatttc
    13561 tcagtaatca aggtgatcag tattggaaaa ttgaaattta gtaaggacag gggacacaat
    13621 tacgcatgaa agtagaaaaa attgttctta tatccgtgtt ttttgggtag gacagtcgcg
    13681 tttgtgattt gtttcgtgtt ataatacaac tagttgtaaa gaaagacaca tcctttatat
    13741 aaaatgcgat gcaagaggtg agacaaaatg agatgtccca gatgtcaaca taataattca
    13801 cgagtaatcg acagccgtca agcagatgat ggacgtgcga tccgtcgccg tcgcgaatgt
    13861 gagaattgtt cgttccgatt cactacattt gaacgaattg aagctgctcc gttattagta
    13921 atcaagaaaa acggggaacg cgaagagttc aatcgcgata aaattttaag aggactcatc
    13981 cgttcagcag aaaaaagacc tgtagctatg gaacaaatgg aacaaatcgt caaccatgtc
    14041 gaaaaccgcg tacgcagtct cggagaaaac gaagtatcga cgaatctaat tggtgaatat
    14101 gtgatggaag atttagttga tttagacgaa atcgcttata ttcgttttgc aagtgtttat
    14161 cgccagttca aagatatgtc tgttttctta aaagagttgc aagatatcgt ggataaagca
    14221 aaagaaaata ataaagagaa taataaagaa aacaattaag aaacaaaaag gaggcatttc
    14281 cttggaaaat gcttggaaag agcttcaacc aaaaaatatt taccaaggaa gaaaagcggc
    14341 tccgctgaga gaagacagca agaacgcctt gctgtatctt tatcagccac taatcggcgg
    14401 agaagccttg agtatctatt tcaccctgtt atcagaaatc tctcaagaat ctggcaaagg
    14461 tcctgaaggg atgcatgcag atctgcttag tagtctaggc tgcggtatcc cgcaatttta
    14521 tgatgcaaga aaaaagctag aaggaatcgg cttactggat gtttatttta aagaagatag
    14581 tgatctggga cctcgtttta tttatgagct gttagaacca ctcattccta aagcattttt
    14641 caaagactct gttctggctt ttcttcttct ggaaaaagtt ggtgaacgac gattccagaa
    14701 gcttacccaa ttctttgaac ctaaaaggca tacagcagag ggatatcaaa aaatcacaaa
    14761 aaaattttta gatgtttatc gcttcaatga agcagtgtac gctgctaatc aagaaaacat
    14821 cgaatcgctg aataaatctt ttgaagaatc tgaatcagta gcattacttg aagcagattc
    14881 gacattcgat tggcagtttt tgaatgattg gctggcaaaa aaacatattg caagtcttga
    14941 tgatccaatc atcaaacaag tcgacatgta tcatcagctt tacggtttcg atgaattgac
    15001 acttggcgaa ctgatcagtc aatcttatga ctttagtgaa caaaaagtat ctgaaaaaga
    15061 actgcaaaaa gccattttgg caatgcagag acgtccggtg aagcaaacaa aagaaacagt
    15121 tgcggtttct gaaaacagta atgaaccctc gtcaaacgaa catttgacaa acgcagcgct
    15181 tcagctgata aaagaagcac agagcattcc accgatgaaa tatctggaag caatcaagtc
    15241 agaaaaaggc ggatatgttt ctaaacaaga aaactggtta ctgcaagatc ttgtttctca
    15301 atcaggtctt tccagtagcg tgatcaatgt tttgctgaat tatgtactag tgatcaagaa
    15361 taatgctagc ttaaatgcca gttatgtaaa tacagtggca aatgaatggg cacaaaagaa
    15421 aatcgccaca gcagaagaag cgatccagca cattcggcaa ataagtaatc aagcaaaaca
    15481 atcaaaacaa aagaagcaaa caaattatca aactggcaag cgaaatgttc gacgggaaaa
    15541 gcttcctgat tgggtcaacc agccaaaaga tgaaaaacag atcagcagcg agaaaaaagc
    15601 agagattgac cgccgtttta aagaatacct ggcaaaaaag gagggtgata tctagtggaa
    15661 gacgtaggca aagaattgaa gaggatcatc agcaaacgca attatcaaga aaagtatgct
    15721 gaaatgatcg ctgaagtctt aaatgatgcg gatgttcaag cttttctaag cgcacaccaa
    15781 gaagaattga cacaagcgga tattgaaaaa agttacgcta aattgtatga attcgtgcaa
    15841 gaaaagcgta aatatcaaaa aaacgatcca acgatgatcg ctcccggcta tgagccgaag
    15901 ctgacgttga attttcatta tgtggatgtc acttatgtac caacggaagc attgttagca
    15961 cggcaaagac aagaagaaat cagaagccga gtaaaagcaa tggatatgcc aaaagatatt
    16021 caatcggctt cttttgaaaa ctttgaacgt acagatggac gaggatatgc gagcttagaa
    16081 gccctagatt ttgtagagaa atatactgct gatccaaaag ggtatcataa aggactgtat
    16141 ctagtaggaa gttttggtat tggaaaaaca tatttattag gagcaacagc gagagaatta
    16201 gcgattgctg gattcaccac gacgctagtc cattttccga catttgccgt tgagatgaaa
    16261 caggcaatcg gaaaagatca agtggcagaa aagttagatg cagtgaaacg ttcacctatt
    16321 ttgatgatcg atgacattgg tgcagatgcg atgagtagtt ggatacgaga cgaagtattc
    16381 ggtgttatcc ttcaatatcg gatgcaggaa cagttaccaa ccttcttctc atcgaatttt
    16441 acaatggatg aattggaaca gcatttgtcc gtcactcaac gtggagatga agagccgcta
    16501 aaagcgaaac ggataatgga acgcatacgt tatttgacaa aagaaatcga aatgactggt
    16561 cgcaaccgac gcaatggttg aacagttgac aagtccaact gacatttgac ctagtagtaa
    16621 aagcaggtca gcgaatcatt atatggacag aaatgaccgg agacaatcac cgcaacaagt
    16681 aatgcagaaa cttaaacgga ggatttttta tgagggctat tttttctagt atatctgtag
    16741 ttggtctatt ttttctttta gcaggctgcg gtagcgcaga tagcaacaca ggttctgttg
    16801 attcgattga tgcgggtcaa acagtaaaaa caagcagtac gaaaacggag agacaaagca
    16861 tatttgtcgg aacgattgaa agtatcactc aagtagaagc agaagaagaa acgcttcagt
    16921 tgaaactcgt cgatgtcatg gccaaagaag atccagcgaa tatgggaacg tcattcagca
    16981 atgacggagt gatcttaaat gctactgctg aaaaaataag cgtggacata aatgaattga
    17041 aaatcggtga caaagtccaa tttacattag ttgagatgcc gatcatgacg atgtctatcc
    17101 caccgcaagt tcctggagat tcgattattg atgtttcatt gaacaaataa ccaataacat
    17161 tttttcaaaa ataagaaaat gttgttttat agaacgaaaa ggtatatttt ttaaggagat
    17221 atgggaattt ttttattcct agtctcctta ttttatttgg aaaatgctat aattatctag
    17281 aaggagtgaa gctatgaacc atttagattt atctcagcgc ttagccgcag tgggtgactt
    17341 tgtccctgaa tccgctcgtt tagcagacat tggatcagat cacgcttact tacccgttgc
    17401 attgatatta agaggaaaaa tcgaatatgc agttgctggt gaagtcgttc aaggtcctta
    17461 tgaatcagca cgtaagcaag tcaaaaaaga tggattagaa gaccgtatca ccgtacgcct
    17521 tgcaaatggt ctagatgcta tccagccaca agatggtatt acagctgtca cgattgcggg
    17581 tatgggaggt tcactgatcc gagatatcct agaagctggc aagttgaatc aacgcttaag
    17641 cggacaagaa cgtttgatcc ttcagccaaa catcggagag cgtacattac gatcttggct
    17701 gcaagaaaat cagtataaaa tccaatcaga aaaaatactt gaagaaaata aaaaaatata
    17761 tgaaatcatt gtagccgaaa aagaacaaaa accagttact tacaatgaaa tggagctgat
    17821 gttcgggcca tttctgcttg aagagaaaag tgagatcttc aaatcaaaat ggacaagaga
    17881 gcttcaacaa agaaaagcta tccgtgaaca gctcaaacaa gcagctaatc aagaggcacg
    17941 tcaaaaacaa atcgagcagg aaatccaaat gattgaggag gttttaaagc catgacaatc
    18001 agcggacgca ctttcatcca aaagtttgaa agctattgtc ctcagtggct agctgaagaa
    18061 ggagacccag tcggactgca tatcggaaca ttggataagc caatccaacg agtaatgatg
    18121 acattagacg ttcgtccaga agtggtcgaa gaagcaatca agaaaaaaat cgatttgctg
    18181 attgctaaac atcctccaat ctttcgtcct gtaaaacggc tggttactga tcagccacaa
    18241 gaaaaaatgt atgcagattt gttgaaacat gatattgccg tctatgcagc acacacgaac
    18301 atggatatta tttgggatgg actgaatgac tggttttgtg aacttctcgg tattgaagtg
    18361 gagagttatt tagtaaagac tcatgagatt cattataaaa aactggccgt ttatgtacct
    18421 gtggatcatg cacagaaaat gcgtgaagta ttagctgcag ctggtgctgg tacacaaggt
    18481 gattataccg gtacaagttt tacctcgatt ggacacggtc gatttactcc ggaagctggt
    18541 gcacaaccag cgattgggaa agtagggaag acagaacaag ttcaagaggc aaaagttgag
    18601 gttattttgc cggaaactat cgaaaaacaa gtgattcaag ccatgcggtc agctcatcct
    18661 tatgaggaac ctgcttatga tcttttcgct atagatgaac cagtcgaaat gtttgggtta
    18721 ggacgagtag gtgaattgcc gcaagaaatt tctatagagg catttgtaga acaagtaaaa
    18781 gaagcgttcc aactagatgg actgagaata gtccagccaa aaaatgcaaa atcctctgtg
    18841 aagcgcattg cgatttgcgg tggaagcggt gaaaaatttt atccgcaagc aatcgcccaa
    18901 agagcagatg tctatatcac gggagatatc tattatcata cggctcatga tatgcaaagc
    18961 gctgggttga ttgcaattga tcccggtcat tatattgaat cgttatgcaa gcaacgattt
    19021 atcgagaaat ttgaatcttg gaaacaagaa gaaaattggg atattaactt tttcgtttct
    19081 gaaacaaata caaacccatt tcaatttaat taaaaggaga gaatactatg tacgaaaatc
    19141 ttttgccacg ttttttacga tatgtcaaaa cagaaacacg ctccgatgca acaagcaaaa
    19201 cgactccatc gactcagaca caagttgctt ttgcgcaaga attgaaaaaa gaattagaag
    19261 agttaggctt agctgatgtt ttctataatg aagataacgg attcgttatg ggaacattac
    19321 caagcaatgt tgatcatgaa gtgcgttcca ttggatttat ttcccacatg gatacagcag
    19381 attttaatgc agtaggagtc aatccacaaa tcattgaaaa ctatgatggc gagtctacga
    19441 tcgtactaga tgcggaagaa cgctatacat tgaatacaaa agattttcca aatttgaaaa
    19501 attatgaagg ccacacattg atcacaacag atggcagtac attactggga gcagatgaca
    19561 aatcagggat tgcggaaatc atgactgcaa tggagatctt gatcaataat ccttctatcc
    19621 ctcacggtac catcaaagtg gcttttggtc ctgatgaaga aatcggtgtc ggagcggata
    19681 aattcgatgt tgagcaattc gatgtagatt ttgcttatac agtcgatggt ggtcctttag
    19741 gtgagttgca gtatgaaacg tttagtgctg ctcaagcaaa tattacgatc caagggaaaa
    19801 atgtacatcc agggacggca aaagatacaa tgatcaatgc tctacagtta gcaatcgatt
    19861 ttcataatca gcttccagcc gatgaagttc cagaaaaaac ggaaggatac gaaggtttct
    19921 tccatttgat ggcattggat gggaatcctg aagaagcttc tatgagttat atcattcgcg
    19981 atcatgatcg tgaaaaattc gaagcacgca aagcacaaat cacacatatc caagaagagt
    20041 tgaatcagcg atttgatcaa gaacgaatca aagtagatat gtatgatcaa tattacaaca
    20101 tgaaagaaat cattgaaaaa gatatgagca tcgtggaatt agcgaaacaa gcaatgatcg
    20161 agcttggtat ctctccagtg atcgaaccag ttcgtggagg aacagatggt tcaaaaatct
    20221 cttacatggg tattccgacg ccaaatctat ttgccggagg agaaaatatg cacggacgat
    20281 tcgaatttgt ttctttacaa gtaatggaaa aagcaacaga cgtgattgta aaaattgcag
    20341 aattgaacac aaagagttaa aatcgaaaaa gaacagctta tgacaaggcg ttcgtcccaa
    20401 tgatggaatg tcatctgtct aaggctaaag ggcaggacaa tggaattggt tgtcccgccc
    20461 tcttttttga agagtaaata ggaggtaaag aatggaaaag ctgctagttt tttataacaa
    20521 aagttcaggg aaagatgagg gtgagaaatt agcacgctgg tttcaagatt atgtaaaaga
    20581 aaagcagcca gaattatatg tagaactagc tgaaactggc ccatcgattg atgatgcgga
    20641 attagtcaag aaagcagaag ccatagaagc tgatacttta gtaattatag gtggagacgg
    20701 cacgattcat catatcgtcc aagcgttcca aaaaacatta tctcaatacc atgtcggatt
    20761 acttccaggt ggaactgtca acaaccttgc acgtgttctt gagattcccc tcaaaaaaga
    20821 agaagctgct gacgtgatcc tggaaggcaa tgtacggaaa atagactatg gtcaagtcaa
    20881 tgatgaagtc attatctcta cactgacaat cggaatctta gcagatacag cagtttggat
    20941 cagtcaagaa gaaaaacagc gttacggctc gtggatcttt atcaagaagt tttttaagtt
    21001 attgctgaag aagaaaaaat atcatttgga tttagaaaca gacgaggagc attggaaagg
    21061 aaaatcccaa ctgttgacag tcacgatgtc taactccgtg ggcggtttta caaattttga
    21121 cgactcagcg accctagatg atggaaaatt tcatgtaacg atcgtacctt ccttagatat
    21181 tttccgattt attttttatt tgcctagaat catgagaggc aaattctata cgatccccgg
    21241 cattacttat ttcacagcca aacgcatgaa aatccaagcg caggaaaaat ctgtgggcac
    21301 tcgaacggat ggagacacga cagatgatct tccagttgaa ttatctgttg tatcaaaagg
    21361 attaacagtt tattcaccaa agaaagaaga aaaagcttag aaatagtagt taacagaaag
    21421 ttggaagaag agtaatggat gtattgttac acaatccttt gtggttttac cttttcattg
    21481 tgaattttta cttattttgt ctgatggggt atgataaaca tcaggctaga actagcggct
    21541 ggcgtgttcc tgagaataat ttgatatttg tcagcctgtt aggcggtggt ttgggaggat
    21601 tgatctctcg aaaagttttc catcataaaa ctcggaaaaa gaagtttact ataggattcg
    21661 ctgcaggtgt acttgttgat ttgattctca tttatttttt ccattaagga gcaggagatg
    21721 tcgtctaaag gaacgaaaac taaaatcatt ctgaatctgc ttttgctgat tgtgattttt
    21781 gccattattt attatttaat caatcaatca tttgcagata tctttgccga attgctgtct
    21841 accagtgttg ttgttttggc agcggctgtt atattaggga cagtttacca aatcgctgaa
    21901 gggcgctcaa tcaaagaaat tgctgaacct ttcgcgccga attttacaac gaaagatggg
    21961 ttcttgacct ctagctatat tgcgttttac cgaatcgttt cgtttggtac aggaacgctg
    22021 atctcggaga tttattttta taacaaaaaa ggaatgaagc tatcccaggg agcaggggtg
    22081 acagcactcc atatgattat gtacaaactg gcagtgatca cttatgcatt agctggcttg
    22141 gtcatccaat tctctctttt ttattctaat ggtccatcaa tgatctggtt tatcatcgct
    22201 ggtatccttt tgacgatcgt gattgttgcc tttttattgg cgatgtccgt cagcttgaat
    22261 gttcaaatat tttttgtcag cttatcgaat aagttattta aaagtgagcg tttgcggaaa
    22321 tgggtggata catgcaatca gcagatctac tctttaaggg aaacggtcca aaccattatc
    22381 caagacagga ctgcgctatt gcggatttat ctatggaata tgttaaaact tgtattttgg
    22441 tatgtacttc cttatttgat tctgattgaa aaccatccta aaatagattt tctactgacc
    22501 atttcgttta tcagctttgc agtagtgctt tcaggggtta ttcctacgcc tgctggtatt
    22561 ggttcctttg aatttgtcta tttgttatta ttcagaccag ttgttggaac agttgacgca
    22621 gtttcttcgc ttttactgta tcgtttcagt tcctttatct tgccatttct ctatggattt
    22681 gtgtatgtga ttgctgaacg gcgaagagtg atcaaagaag aaataataga agtaaaaaaa
    22741 gagaataaag aaaatcaagg gtaggtcttt tataagatct acccttttta tcaaatcaaa
    22801 gaaaaacgtt atggataaga taattttacg tt
//
LOCUS       DAZULO010000036        22031 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000036 DAZULO010000000
VERSION     DAZULO010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.037"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            367..555
                     /locus_tag="ACG8OA_001401"
     CDS             367..555
                     /locus_tag="ACG8OA_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976194.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="ACG8OA_001402"
     CDS             545..805
                     /locus_tag="ACG8OA_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6275 family protein"
                     /protein_id="HFS4976195.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            846..1073
                     /locus_tag="ACG8OA_001403"
     CDS             846..1073
                     /locus_tag="ACG8OA_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976196.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1138..1710
                     /locus_tag="ACG8OA_001404"
     CDS             1138..1710
                     /locus_tag="ACG8OA_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976197.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="ACG8OA_001405"
     CDS             1711..2022
                     /locus_tag="ACG8OA_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS4976198.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="ACG8OA_001406"
     CDS             2013..2273
                     /locus_tag="ACG8OA_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976199.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="ACG8OA_001407"
     CDS             2434..2793
                     /locus_tag="ACG8OA_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976200.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="ACG8OA_001408"
     CDS             2790..4433
                     /locus_tag="ACG8OA_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS4976201.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4468..5550
                     /locus_tag="ACG8OA_001409"
     CDS             4468..5550
                     /locus_tag="ACG8OA_001409"
                     /inference="COORDINATES: protein motif:HMM:NF028182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage abortive infection protein"
                     /protein_id="HFS4976202.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5594..6766
                     /locus_tag="ACG8OA_001410"
     CDS             5594..6766
                     /locus_tag="ACG8OA_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4976203.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6753..7457
                     /locus_tag="ACG8OA_001411"
     CDS             6753..7457
                     /locus_tag="ACG8OA_001411"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFS4976204.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7462..8622
                     /locus_tag="ACG8OA_001412"
     CDS             7462..8622
                     /locus_tag="ACG8OA_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS4976205.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8698..8976
                     /locus_tag="ACG8OA_001413"
     CDS             8698..8976
                     /locus_tag="ACG8OA_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HFS4976206.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8957..9328
                     /locus_tag="ACG8OA_001414"
     CDS             8957..9328
                     /locus_tag="ACG8OA_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HFS4976207.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            9344..9748
                     /locus_tag="ACG8OA_001415"
     CDS             9344..9748
                     /locus_tag="ACG8OA_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976208.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9745..10155
                     /locus_tag="ACG8OA_001416"
     CDS             9745..10155
                     /locus_tag="ACG8OA_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976209.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            10216..10803
                     /locus_tag="ACG8OA_001417"
     CDS             10216..10803
                     /locus_tag="ACG8OA_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFS4976210.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10876..11343
                     /locus_tag="ACG8OA_001418"
     CDS             10876..11343
                     /locus_tag="ACG8OA_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HFS4976211.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            11389..11697
                     /locus_tag="ACG8OA_001419"
     CDS             11389..11697
                     /locus_tag="ACG8OA_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976212.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11892..18626
                     /locus_tag="ACG8OA_001420"
     CDS             11892..18626
                     /locus_tag="ACG8OA_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4976213.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18657..19502
                     /locus_tag="ACG8OA_001421"
     CDS             18657..19502
                     /locus_tag="ACG8OA_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFS4976214.1"
                     /translation="MNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSFREIGESIEL
                     SEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSLKSLSKTEID
                     SQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYTKNVWEKKGV
                     FKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFINMKDTQILE
                     VSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGHANVEIELYE
                     ERDLF"
     gene            19499..20548
                     /locus_tag="ACG8OA_001422"
     CDS             19499..20548
                     /locus_tag="ACG8OA_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976215.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            20552..21376
                     /locus_tag="ACG8OA_001423"
     CDS             20552..21376
                     /locus_tag="ACG8OA_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976216.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21394..>22031
                     /locus_tag="ACG8OA_001424"
     CDS             21394..>22031
                     /locus_tag="ACG8OA_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFS4976217.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         7878 a         3297 c         4671 g         6185 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tcaaaatgtt tcaccgaata
     5161 gttaaaacat taaatgagta ttatgacgat tataatgatt ttgatgtgaa gaggtataca
     5221 aaatatatag ggacattaag aacacaaatt tctccagctg aattccaagt gattttattc
     5281 aattctctat atataaaacg aggttttggc ttaggaattc aacttattgg aagtggtttt
     5341 tttggagatg attttgattt tgaaactaat caacattttg aaacaagtat caatgaacag
     5401 tggtttctat cactatcaac tgttgacagt gataactcta taaagcgaca aaaattatct
     5461 gaatatatta aagaattagg atccgtagaa tataaaaaaa tagctaattt tgaaagttta
     5521 tataaactct ttaatgaaac taagctctag aaatagggct tttttatttt tctttgaaag
     5581 gtggtgaaaa catgtgagct tatttgatat ctttaagcag tcaataagaa atgaagagcc
     5641 ttctgagtgg ataccggatt ttgtatatgg agaggaagta tctgctcggg catacctcaa
     5701 aattatggca aaaaatactg ttttagattt tgttgcaaga actatgtcaa cgttagaagt
     5761 taagtttaaa aataaagatg ggacatcaga ctgggattat attctaaatg ttcgacctaa
     5821 ttctgatatg tcagcagcta cattttggca aaaatttttt tatcggctct tagatgacaa
     5881 cgaagtgtta gtaatattta cagatgataa tcaattgctg attgctgatg attttagtcg
     5941 aacggagtat gccgtgtatg atgatgtttt tacaaatgtg atggtaaaaa attttgtctt
     6001 tgaacgttca tggaacatgt ctgacgtcat ttatatggaa tataataacg aagaattaga
     6061 aaggtttaca cgaggtttat ttgaagatta cgccgcgttg ttcggacgga ttctagaagt
     6121 tgcaatgcgt aacaaccaaa ttcgtgggtc tgtatccatt gattcaacgg gaacggcgaa
     6181 tgatgaaaaa ggaaaagatg gtaagactag atctcaaaga ttacaagaat atattgatag
     6241 gatttggaac tcttttaaaa ctaactcagt tgcaattgtt cccaaagtga aaggctttga
     6301 gtatgaggaa tacaccaata aacaaggcgt atcgaatcaa tcgctagatg aactgaataa
     6361 gatgaagtcg tctttaattg atgatgttgc caacgccata ggagtaccta cggcgcttat
     6421 ttatggtgaa aaagctgaac tggattcaaa tattaaagct tttcgtaaat tgtgtattgc
     6481 tccactagtt aaaaaacttc aagacgaatt ggtggcaaaa ataattagtc gtcaagagta
     6541 caaaaacggg gaacgtatag aagttaaaaa agttctccaa aaagatctat tagagtatgc
     6601 tactcaaatt gataaaataa catctggtgg tgcgttttat attgacgaag ttcgtgatga
     6661 agttgattat gatgagttgc caaataatga aggaaaaatt cgtattcgta cgaaaaacta
     6721 tgaagaaact gtgaagggag gtgagaacga caatgccgaa agctaaaaaa gtaccatttc
     6781 agtttactaa cgaaattcag aatggtaagc acattctcac cttgagtgga aatgtacaga
     6841 aaaaatattg gcgtgatgat gatgtcataa acgctaaaga tattcgtgac tctctagata
     6901 ctgtaaccga tgatattgta atcaagttga acagcccagg cggtgatgtg tttgaaggga
     6961 ttgagattta caattattta aaagatcacc catcaaatat tactgtagaa gttacagggc
     7021 ttgccgcaag cgcagcaacg tttattgtcg ctggtgccaa tgatgtgatt atgaatgttg
     7081 ggacttcttt gatgattcat gaagcttcta catttgcttg ggggaataaa caggacatcc
     7141 aaaaaacgtt aaacgcattg gaaacgatcg atgattctat cttagctatt tattcagata
     7201 agactggcca atctgttgat aagttgcgtg aatggatgaa tgaagaaaaa tggtttacag
     7261 cagatgaagc tgtcaaatat ggatttgcca attctgtgaa gcgtgaagaa ccacaaaatg
     7321 aacctcaaaa tattgcagcg atgattcaag atgctgttgc cgttgcaata gcgaatttaa
     7381 aggaacctgt tactaataaa atcgaacaag aatcaaaaca aaaatcatta atcgcacgat
     7441 tgcgaaaagg agaataaaga tatgttaaaa attacagata aaactgcaga tgcaaaaaag
     7501 gcatttaatg ctatttcttc gaaagaagat gcaacacccg aacaaattaa tgctgctcta
     7561 gaagaatatg taaccgccat tgcagaagat gccggcaaac aggtgcgtgc tgaatatgaa
     7621 gaattgaaaa atgtgacgga caaccgtgta ttagaggctc gtggaattcc tacactaaca
     7681 gcagaagaaa caaagtttta taatgaagcc gtaaaatctg gaggtttcga ttctgacgta
     7741 gtttggccag aaacgatttt ggaacgtgtt tttgaaaatc ttcaaaacga tcatcctatt
     7801 ttaggcctca ttaactttac accaactgta ggtcgtgtca aagtaattag agcacgtcgt
     7861 aaaggtgttg ctgtttttgg accattgcat aaagaccttg aaggccaact agatgctgaa
     7921 tttggtgcaa ctgaatttgt tcaacttgcg ttgaccgcat ttttcttaat ttctaatgat
     7981 accttagatt taggaccacg atggattgac cgttttatca atctttcttt aagtgaagca
     8041 gtacgtgatg tttgggctaa aaaaattatt acaggttctg gtaaagatga gccaattgga
     8101 ttgcttaaag atctagatgg tgcggtcaca agtggtgtat atccagataa agctagcgca
     8161 gggacattaa cttttgcgaa agagaagatt gttcttgaac ttgcaggggt gatgaaaaaa
     8221 ttatctaaat acacatacaa agtagatggt gacgatgaag gtgaagtgaa ataccgttct
     8281 gttgctggac gagtgtatct aattgttaat cctgttgatt actgggatat cgtagctaga
     8341 gttacatttg caaatttaaa caatgtgtat gggactacga tgccatttat cgctgttgat
     8401 catatcatcg aatctgtaga cgtaccagaa agtaaatgta ttgcatacgt tgataaagag
     8461 tatgaagcta ctcaatcaag agctgaaaaa gtctatgtat ataaagaaac ttttgcaatg
     8521 aaacgcgcaa ctttgtatgc tgtcgacatg cttggtaatg gctatccaac aaataatgat
     8581 gcagcacaag tatatgactt ggactttaca gcgggggagt aatgtcccct gcaattggtg
     8641 agataacacc gacgactgat ggggcagtca ttaatctttc gtaaatgggg ttagagtatg
     8701 aacactgaag aatttataaa agaatataag gcccgatttc gtattttcca tacttccgaa
     8761 gatgaggata tcgggaaaca gttagagagc gggtttgccg atataaaatc tctaatcggc
     8821 agttttgatc cttccactta tgagaaagga aaagaattag tttatgaacg tactcggtat
     8881 ctacggaacg aatcactaga gtacttttac gacaattttc aaacaatgat tatggatgca
     8941 tcaattgatc tggcaggtgc tggatatgcc aattaaatca aattataaga agcctaaaat
     9001 tgtagctggt gatctaaata cgccagttac tttttttgag tttcaaccta ataagggtcc
     9061 agaacctggt gatgaagaaa aaaaggaact gtatttttgt acagcactag tttacaaccc
     9121 atcaatgaaa gatcgtgaca ttcttggcac aaaagaaggc gttacgataa aaatacggga
     9181 tccccatcaa tcttatattc ctactaacaa acataaagta aggatattag attatcgata
     9241 tggagataaa gtttgggaaa taatcgatgt ctcaccggat ttggaagaaa attcaattgt
     9301 aaagatagta ttagaattaa ttacttaaat ttggtgataa atgatgagta acgtaaagtt
     9361 tgaaggcgtt caagaaacaa tccaagcttt agaaaaaaag tatggagaaa agaaaactaa
     9421 gacattaaca aaaaaagcaa tcaatgttgg cgctgaaaaa gtagaaaaac agttgcaaac
     9481 ggacatgacc gtttttaaag acaaaggtta tacgatcgct gaggttgttc gcaagaacgc
     9541 tacgtataga gattacaagg cagaagctga aatcggttgg aatggtccac atcaacgata
     9601 tagaatcatt catttgaatg aatggggata tacacgaaag ggcaaacaaa ttagaccgag
     9661 aggatttggt gtaataacaa aatctcttaa gaattccgag ctgttgtact ttgatgctgt
     9721 aggagaggag gttaaaaaga gtttatgaaa gatatgctta atatcattta cgaggctctt
     9781 tgtttgaatg agtatattca ttcaatgacc tacaacgaga ataccgagca atatcgtatt
     9841 aaattttatg agcaacctga aaccgccgat aaaacaggtc caatgatcac aattcgtcct
     9901 gtagatacac ctaacgaagc gtaccacgga agtaacaaag agctttctgt ggagcatatg
     9961 atacagattg acgttgaatc gaaatataga gcaacatgca agcagattca atatgagata
    10021 aaaaaagaga tgaaaaaact aggctttggc caaatttccg gtcaagggtt agatgaatac
    10081 tttactgaaa caaaccgctt tgtagacgcg cgtcgttacg aaggaaatac aaaaatttac
    10141 gataccgaat attagaaatt aagacacgaa aataacgtgt ctttttttat tacccaaaat
    10201 taggaggaaa attttatgac tttagtagga ttcaaaaaaa tgacaatcgg ggtctttgac
    10261 agcacaggga agattccagc agcaaattta tatgtaattg aaggtgaaca aaacaaaggg
    10321 gcgactgtat ctgctgaaat cagtgggcta tcaaaagaac catcaaaagt ttacggatcg
    10381 aacatcgctt actatgtctc tcaaaaagga acaggcgatg tttcagctac ttttggattg
    10441 ttagatttac caacagaagt aaatgacaaa attcttggtt acaaaaccga tacaaacaaa
    10501 atcagcttct tgggagaaga tactgagcca ccatattgtg caattttgat ggaatcggag
    10561 gatttgaacg gcgatacagc aatgcttgcg atgttcaaag gaaaattcag tcgtgaatct
    10621 attaatctta acactacgac aaatgaagct ttcgaaccag aagcagaaga atatgtattt
    10681 tcagcgattg ccaatgatgt tgaaggtgat gctaaaggcc aaacagtcgc aaaatatgtt
    10741 ggagacgaag aagcttctat tactggtttg aaagagatgg tatttcctgc agcgggggag
    10801 taactagccc cgttgttgga gcaatcactc ccactattaa tggggcaaca gttgaattaa
    10861 gttaggagga taactatgcc agatacattt aaaatctata aaaaaaatgg tactgaattt
    10921 acaaaagtcg cagagggtga aagtccactt tctattactg gaattgcagc aaatactcaa
    10981 gttgctaaag gagattatca agctacgcgt gtggtcggag gtcaagaatc agtaaaagta
    11041 gatattcctg cattcaccac tcttccaatt tcagttactg gtgtgacgtt ggataaaacg
    11101 acagctgaag ttgaacaagg cggaacctta aaattaactc ctactgtaac accagcaaat
    11161 gcaacagaca aaacaggaag ctggagcagt tcgaatacag ctgtagctac ggttactgga
    11221 ggcaacgtta cagtaaaaac agacgctgaa gttggcggaa ctacagaagt ttcatttact
    11281 actacggatg gcggtaaagt agcaaaatgt actattacag ttattgaaaa agcagcgggc
    11341 tagtcttcgg actagctttt ttatttggaa aataatggag gaatctaaat ggcaaaagta
    11401 agaattgaat tgaaagaaga atcaggaaag aaagtctatg aaaacataga cacaacaggg
    11461 aaagattatc gaaaagcttt agaaacaatc aaaaagctaa atgaagacgg agtaatgatt
    11521 tggaatcgtt tagacattta tttggatttt gctgtaggta tcttcaggga tagcaaattg
    11581 acagctgaac aaattcttga tggcttacca tctgagaagg taatgagcac attggacgac
    11641 atcctaggag aagtaatggg gattgaaagt aatccagacc cagacgcaaa aaagtaacac
    11701 ccgaagaagc agaagaaatg tacttaaacc tatgcagaga gttagtgaaa gcgggatggt
    11761 cactatctga catagaagat aattcctttg aaatgatgat gaaaattgcg tgcacttcgc
    11821 ctaagaaaga gaaacaaaaa gaagttgatc ttaaagactt cttgaaatcc atgtagagag
    11881 gaggtaaaca tatggcaaat ggaaaacctt taggcaatat gattatcaaa ctagacttag
    11941 acagttcagc tttttcaaaa ggtctagccg gagctaaaaa cgctgttaac catcaaatga
    12001 aagcaatgaa atcacagatg caagtaatga actcttcagg aaattctctc ggtgctttac
    12061 aagcaaaata caatggttta ggcggagttc tacaagcaaa tgaaaagcaa gtagaattgc
    12121 tcactaaggc ttataaggat agttttgact ctaacggaaa cgctactgct tcgactgcta
    12181 aatacgctaa tcaattgaat caagcaacag ctagatcagc aagttacgaa tctcagatga
    12241 aaacaactgt tggacagatt gctcggatga aagtggaaac ggaagggttt acggggaaac
    12301 taaaggctaa ttctgatgta ttgattaatt ctgggaaaaa aattgaagcc tttggtaaag
    12361 gagtttctgg gataggatct gccctaactg ttggagtaac agctccaata gtggcaggcg
    12421 caactgctgt tactagtgca gctatttcat gggaaagtgc ttttgcaggt gtaaaaaaga
    12481 caaatgatga agttgtagac tcgaatggaa acgtagttta ttcctatagc gacttagaga
    12541 aaggtctgcg taatcttgcc acccaattac ctggtagcca tcaagaaata gcaaatgtag
    12601 ccgaagcagc cgggcaatta ggtataaaga ctgaaaacgt agttagcttc actaaaacga
    12661 tgatagacat gggtgaatcg actaatatgt cggctgaaac tgcggcaact tctttagcac
    12721 gatttgctaa tattacccaa atgtctcaaa aagactttga taaattagga tcagtcattg
    12781 ttgatctagg taacaatttt gctacaaccg aatcagaaat cactgaaatg ggattgcggt
    12841 tagcgggagc tggtaaacag attggaatga gccaaggcga aattctagga tttgctactg
    12901 ctctatcttc tgttggtgtc gaagctgaag cgggtgggtc tgccttttcg aaggtcatga
    12961 tccagatgca actagctgta gaaaaaggat ttggcgcttt tgatcagctg aaacagatgg
    13021 cagaagaaca aggtgttccg tgggttaatt tagtaaatgc tgtgagagac ggtggaaaat
    13081 cgttaaaagc agtatcagag caaatgggat tcacttcatc tgatttaaag aaaatgtata
    13141 aagaagcgga caattcgaaa agttcgcttg aaaattttgc gaatgtggca ggtgttacaa
    13201 gtgaacaatt ccagaagttg ttcaagagtg atccttcaga agcaatcatc aagttcattc
    13261 aagggctgaa agattcagaa aaacatggta cttcagcaat caaagttctg gatgacatgg
    13321 atataaaaga agttcgacta cgcgacagtt tattgagagc tgcaaatgct agtgacatat
    13381 tcaaaggagc aattgaaaaa ggaaacggcg cgtggaaaca aaatacagct ttaacaaatg
    13441 aagctaataa aagatacgaa acaactgaat caaagctaaa gatattgaaa aacgaagtga
    13501 ttgatacagc aatcgatctt ggaggtccat ttgtagacgc gttgcgtgat ggtctaaatg
    13561 tatcgaaacc attgataaaa aacttaggga atatggcgaa agctttctca gctatgagtg
    13621 atgatcaaca aagaaacatt atcaaatggg ttggattagc agcagcagct ggtccagcgc
    13681 tgaagctttt aggaactggt acttcagtaa taggtaaagt tacttcaact actggaagat
    13741 tgacgaaaag ccttgttgat ttagcagcga aagcagcaga aaagaaggct atggatgcct
    13801 ttgcatcgtc ggtaacaaca gctggtgctg cagctgcaaa gacagcagga gctggaggta
    13861 ttggcgggtt agtccctatg atgggaaaaa cagctagtgc tgcaagtact gctgcgggcg
    13921 ccagcggaat aggcgcaatg acaacgtcac taggtttgtt aggtcctgct ctacttggta
    13981 ttgttggcgt tggtggcgca ctggcagttg gctatggagc atggaaatta tttggtgaag
    14041 aagcttggaa ttcctcgcaa agagtaaagc aatggggaac agatgtcggt gaggagatag
    14101 atagtactct tgacggagta cagaagaata ttgaaggtgc aaatggtcaa ttcggtcttc
    14161 tcaaagaggg ttttactgtt gatgatgcaa ataatatggc ggctaatttc gcaaccgctg
    14221 gaacatcttt ggaaacatct ttaacgggta gaataaaggc attagacgat gcactaaaag
    14281 ggctacctca atcagttcag gatgccatga aagaagtagc tgaatcagaa gaagaaacct
    14341 tagggcaatc tctttctaaa ataaaagaca acaatgaaga aataaaaaaa atcagagaaa
    14401 gagccgcaaa tcagaacaga gacttgactg tttcagaact acaacagata caggatctaa
    14461 tgaaaggaac ttctcaagcg tatgtggata cgctcgatgt ttctgctaaa gacagaaaaa
    14521 agatactatc agccatgaat ggtgatgttg cacaagctac agaagaagaa gcgaagacgt
    14581 ggttacaatc tttagcgaaa caaaaagctg caactaccga aaattataga caaatgcttg
    14641 aagaaaaaaa ggcggcttta aaggaaaagg gagtaagcaa agaattcatt gatgcattag
    14701 aagatgaaat gaatgattac atcaaaacta caaatcaagg attcgatagc caaatagctg
    14761 ctataacaaa taaatatcca caattgatta acgaagtgag tttatcaaat ggtcaattat
    14821 ttagcaaaac ggcagctgct atggacaata ctggtcagta tacaaaagtt tttctcgaaa
    14881 gtaatgaaaa gattttagcc caagcgaaga aaacatcgga tgaaatagca gataatgccg
    14941 aaaaaaatgc agaacaactt ggtatggtag ctgatgaggc tacgcatgct ggatcaactt
    15001 ggaatggctt agttttagac gaaaaaacag gaaaagtaaa aactaatgta caagaagtag
    15061 tgaatgaagc aacgaaggat ttcactaaat ggaatgaatt gaaacctgtt gttcatgatg
    15121 cgaaactaac aacgaatgca aaagatgtca tttccatagc ggcagtacaa aatggctatt
    15181 gggatgatat ggaatggaaa gaaaaagaag cattattaga agatgagtgt tctaaaaatg
    15241 tatacaaggc tttagaaaat gctggtaaat ggaatgagtt agatattcct tctaaagaag
    15301 caatcctaac ttcaaatact cctgaagtta tgggtgaaac tctcatgaat ttgggattat
    15361 gggatactta caaacttcaa gtgaaagatt tagatttgaa tgcgtataaa gtatacgatg
    15421 ctatcgatgg atcagaagaa aaattaaata gttgggctca attagataac cccacaaaag
    15481 aattgttatt agataactca gatttttcta caaaactttt taattccgaa gaaatgttta
    15541 aacgttggaa tcagttatca tctaatgcaa agtttctttc ggctaataat actaaattta
    15601 cagaggttct tttatcatct gacagaatgt ggaatcaatg ggtaaacctt cctgataatg
    15661 agaaaagatt acttgcagat aacgaagatt tagctaccac tgttttatct tcagaagaaa
    15721 tatacggaag atggactcaa ttaccagata atcaaaaata tatgttagct gacaatactg
    15781 acttagcaaa caaaatattc gcttcagaag aatcatggaa tgcatggaaa gcaattccag
    15841 acacagttaa aaacttgctt ggtaataata ttgatttact ggctaaagtg aaagacggga
    15901 ctgtaagtgt agaagaatat aacaaagtta tgccacttct taaaactcta tatggagata
    15961 acgttaattt aaaagaggca atcagtgggg ctaaaagctc tatagagaat tacaacaaaa
    16021 atatttttcc tagtgataaa actgcagtag gacacgatga ggcttcacaa gctgctaaag
    16081 atgcatttga agcgttcaac atcttccaaa caaaaatacc agataaaatt actaaaactg
    16141 tatcagctga ctttataggt cctatgccga aaaatgcgaa aggaacaaac ttccatcctg
    16201 gcggagcagc tatggtaaac gatcaaaaag ggcctactta tgaagagctt ataaccttgc
    16261 caagcggaga aacttttata cctaaaggac gtaatgtcgt tttagactta ccaagaggtt
    16321 cgaaagtact gaacgccact aaaactaaac gtctagtgcc taaatatgct gatggtatag
    16381 gaaacataac gacggtttca tctacgctaa acttagatgc tttaatttta gctattaacg
    16441 agctaacgac ggtattgaga actcaactga tggcaggaac gactgatgct aaagatacta
    16501 aactatcaga tgaaacaaaa gttgccgatc cgattattcc ggacagtctt tctgaaaaat
    16561 cagatcagta tctaggaatt ggagctcaat ggttaactaa tctaatgaat ggttggaact
    16621 cagcggttcc tcaatatatg aatagtgaaa tggtttttat tacaaactat ctcaatgcgc
    16681 taaaacttca aaacaatcct aattatacgc aaggagcaac atggaataag aacttactga
    16741 atggatggaa tagtttaact gggaccttta ttgctacaat caattcattt tgtaatcaag
    16801 caatggtcac gcttagaaac tacaacactc ctatgtacaa caatggccga acgtggcagc
    16861 aaaataatct taatggttgg aattcattgt atggatcatt catagcccgt gtaaatcagc
    16921 ttggtaacga ttcgattaac aaccttcgtt ctaaaagtgg tggtttttac aatgcaggga
    16981 cattcttgtt gcaatcttta ataaacggaa tgaattcgtt aggtaattct ctttctacta
    17041 caatgaacaa tgtcgctaat acaatggttg gtggtatagg caaaggagtt aatggcgtca
    17101 tatcgggagt taactatgtg cttaaggaag tcgaatcaag taagaatatc ggaaactggc
    17161 caattccaca atacgctaag ggaactgatg gacatcctgg tggacttgct atgattaatg
    17221 atcaaaaagg cctagttcac gaagaatatg ttcagatgcc agatggacgt agttttattg
    17281 ccaaaggtag agatcttctt gttaatttgc ctaaaggtgc tcaggtctta aatgcttctt
    17341 taacaaagaa attaaaggaa cgtttaaata ttccacacta cgaaaaaggt attggcaatt
    17401 tagatattgt tgatttgtta gatgatgaaa aaagaatgtt agaattctta actagcaaag
    17461 ttgatttttc aggcattaat gaaccatggc ttgatatgac taaatcagga acgtcattaa
    17521 tgtccaaagc tgcaaataca atgcttcaat caaaattaag tgaattcttc actcatggca
    17581 attttgatgg tgcagtaaat gccaatggtg tttatcaata tttagttgat gtggcacaga
    17641 aagtaatggg taagttcccc ggacttacag taacttctgg atatcgagca ggagatgcct
    17701 actatcacgg aaaacggcaa gctatcgact tggcttatcc aggtatttct ggagatccga
    17761 gatatacagc agcagcaaat tacgcttttg aaaaattccc ttcaaaaatt gcgtatgtca
    17821 ttacgaatgg acgtgtacgt gaccgcatgg gattatctgg aacaaggtca agcggacaat
    17881 ggacgaattg gccagatggc gatcacttcg atcatattca tttaaacggt tcgatggggt
    17941 ctggcgatat attcaatgga ggagttggag gaagtggtgt tgcacgctgg cgttcttatg
    18001 tgtctaaagc attgaaaatg aatggattac cagctacagc agcgtatata aatgcctgga
    18061 tgtcccaaat ccaaacggaa tctggtggta atgagaaagc aatcggcggc aatgatgggc
    18121 tcgccgaagg caatgcaaca ggattacttc aaacgaaacc cggaactttt gcagctaacg
    18181 cttttcctgg tcacggaaat attatgaacg gatttgataa catgttagca gcaatcaatt
    18241 atgctaagaa acgttacgga gttgcaggta tgttgcaagt tatcgggaaa ggacatggat
    18301 atgccaatgg cggcttgatt accaaagacg gactatatcg agcaggagaa ggaaataaac
    18361 cagaaatggt tattccattg acaagaaaaa caagagctat cgaacttatg ggtcaagcat
    18421 tagccttcct ttctggaagc aataaaaaag aaacgaaaag tgagattaat tctgtagata
    18481 atagacatat cctacaagcg atagaaaaac agtctcgtat gacaaatgat ctgctatcat
    18541 tattactctc gttttttaaa agtaacaatc gatcagataa agaactagct ttagatattc
    18601 agaaaatttt aatcaggagg acatagtatt tgaggacagt aatattaaga aataaattga
    18661 atgaagaaat tgatttgtcc acagaagatt attttgctac tgaaatgtcg aatctaggtt
    18721 ttgaagttac aaaagagcat attggacaat gggggagttt cagagaaata ggagagagta
    18781 ttgaattgtc tgaatttcaa tcatcagtta tgatttctgt tcacggattt agagaacaac
    18841 aattatatgg ttcattagtt gaatttctgg ctaatggacc ttttgaacta gaattttctt
    18901 ttgattcaga aacaatgatc aggaaatgta gtttgaaatc attatctaaa acagaaattg
    18961 attcccaaag ctcattgcta acagatactt tgaatctata ttttacttca agctggtatt
    19021 ctattaagcg tgaaaaatta atacagaatt caacttctct caatacacgt ggaaaagttt
    19081 actcatttgt tcgaccatat gtctacacca aaaatgtatg ggaaaagaaa ggtgtattta
    19141 agttcagtaa taattctgta tatttgacaa atagtacaga gcgaatgtct ccgttaaaaa
    19201 taaggattat tggagagtgc tcaaatccat attgggaagt tatccaaaac tctaaagtag
    19261 tagcttcaga tggctatttt attaatatga aagacacaca aatattagaa gtgtctagtt
    19321 tgctagaaga ccaaacggca attttaaaag atgtaggtgg agttgaatct tctgtttatc
    19381 aacaacaaga ttttactaaa tcaaacttcg tccaagcgcc tctaggagaa ttcagcgttg
    19441 tttttcatgt tggacatgca aatgtagaga ttgaacttta tgaggagcgt gatctatttt
    19501 gattttagcc attaccctct ttcaccgaga tatgaattta tataacgaac gtttaatttc
    19561 gactgagttc gaatttggaa cggatgaaat tggagaagaa gtaagtaatt ttattacaga
    19621 taaatatgtc catgtaaaat caggagattt tctactagca aaatttattc ctagcgggaa
    19681 gattgcttat tttggagtga ttagctcgca agaagacaaa aaaataaatt gcaaaggatt
    19741 actcagtttg gccgatagcg agataccgac tgtaaaaata tcaggtgcta attatgaaga
    19801 gcatattcga cgactgattg aatactactt attgaacgat ccaacaaaac aactaaaaaa
    19861 tattttggag gtaaaaacag aaagcgttac ttctcattct tatcaagctg ttgacacaaa
    19921 taagagacga ttgaatgcat atataataaa tgggttcaaa aaatataatg ttaaatggta
    19981 tgtcaaagag attaaaggta aaaaaatata tactggtatt cgagcagttg atgattcaat
    20041 ttatattaaa gataattcat ctgagtttaa tgactgggat gtttttgtgc aatctcctgg
    20101 accagggaac gagaacaaat tattgatcgt agataaagca atgaaagata tagaaaatcc
    20161 cttggtattg tctacgtggt atttagatga cgaaaataat cttacgcaag attcaacgaa
    20221 taagaacatt gcattgccta ccgttaattt agtaaatata tatgatcaaa ctcaagaaga
    20281 tagaccttct tatgaagagg tagctaaatc ggagcttaaa ggcaacgcct attcacatga
    20341 aattaaagtt aatgttgtgc taaatgctag aaatatcaat gtagaaaata tcgaaacagg
    20401 aatgttagcg actattgttt acaaagagca gacttacaaa tctgttctaa ctgcctggcg
    20461 aatatccagc agtaaaaaga atatagaatt gacatttggt aatgtccgaa gtcgttttat
    20521 ggattatttt gaagataatg ggggataaaa aatggttagc aatgtggatg gatatcaatt
    20581 tgaaaacgtg aaagtaagcg cagaaaatga tgctagactt tatcacgttt tatataatcg
    20641 gaaaaatcag gttattgatg gttacgatca gtctatgaat ttatcttcaa gcggattaac
    20701 agtaaaagtt gctgcaggag cagcgattat tcaaggtcgt atggtcgttg ttcgacaaga
    20761 agaaagtata acagttccag caaactcaag tggttatata gcattaacag tggatttgac
    20821 acaagaagtt atacctggat ctattcttcc agaatcggaa gaatatgaat ggactaataa
    20881 tcaagtcaag ctagaattta taacaaaagt tataaaaggt aatttaaaca atggtgataa
    20941 ggtctataat ttaccactat gctcagttaa ttctactgga tcaactgttt caatctcaaa
    21001 gatatcggat agttacgagc tgactctctc taaaggagaa attttgtgga gggggactgc
    21061 gttaatgcat gatactcaaa ctgtccaacc ttcaaaaaaa atttggcaga cagtttcagg
    21121 ttttttgtta gtatggctcc catacgaaaa cgggcaagca attgaggata gatatgtaac
    21181 tacgcctttt tataaggagc gtataacttt tactaatgtt ttaggagaaa ttgtttcagg
    21241 atttgatgac tatcacaaaa agtggtttag taaacgaata aactataatt cgaatactaa
    21301 tatatttaca ggtgcagcaa gcaatgcaag tggagataac gcaaatatgg tgcttaggta
    21361 tatagtttct ttttagttag gaggtatgga agagtggcaa atttagaaat taaattatct
    21421 gcaaataaga gacagcctta tctacgtcac cgtgttgttg gtagagttgg tgatgggggg
    21481 ctcacaacaa tcaatgtaca acttcttgag gaagatgaaa ttacaccttt tgtaattaat
    21541 ctaaacggta ctttgaaatt tgtgggcgaa gtttcaaacg gtaactatac cgagggagaa
    21601 ccagaaataa tcgattcgac taatgggtta attagttaca cgttcactaa gtcaaatttc
    21661 agcacgagtc atcaattcaa acaagcatat tttgaatatg tagatcctaa cggcaaaaaa
    21721 gtaacttttc agaacttcat catagacgtg ttagaacgag tagatattaa ttcggagcaa
    21781 gcgaaatact atatttcttc attggaaaaa ttacagagtg aaatgcaaac cactttcaat
    21841 cagttcatta gtgataaaca ggtccaatac gatcaaatct actcgaaata taacgaatta
    21901 gtaagattga taaatgaatc agataagcaa gtaaatgatc gtattgataa ggcaaataat
    21961 cgtattgatc aaaccaatca gcaaatcggc gatctcggca agctgaaaaa gatgtacagt
    22021 aacagcattg a
//
LOCUS       DAZULO010000037        22028 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000037 DAZULO010000000
VERSION     DAZULO010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.038"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..1371)
                     /locus_tag="ACG8OA_001425"
     CDS             complement(61..1371)
                     /locus_tag="ACG8OA_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFS4976218.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="ACG8OA_001426"
     CDS             complement(1383..2801)
                     /locus_tag="ACG8OA_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4976219.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="ACG8OA_001427"
     CDS             complement(3041..4609)
                     /locus_tag="ACG8OA_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFS4976220.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="ACG8OA_001428"
     CDS             complement(4613..4894)
                     /locus_tag="ACG8OA_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFS4976221.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="ACG8OA_001429"
     CDS             complement(4891..5814)
                     /locus_tag="ACG8OA_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFS4976222.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="ACG8OA_001430"
     CDS             complement(5811..6689)
                     /locus_tag="ACG8OA_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFS4976223.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="ACG8OA_001431"
     CDS             complement(6676..8013)
                     /locus_tag="ACG8OA_001431"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFS4976224.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="ACG8OA_001432"
     CDS             complement(8016..9119)
                     /locus_tag="ACG8OA_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4976225.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="ACG8OA_001433"
     CDS             complement(9193..10701)
                     /locus_tag="ACG8OA_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4976226.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="ACG8OA_001434"
     CDS             complement(10667..11359)
                     /locus_tag="ACG8OA_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFS4976227.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="ACG8OA_001435"
     CDS             complement(11381..12355)
                     /locus_tag="ACG8OA_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4976228.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="ACG8OA_001436"
     CDS             12655..13527
                     /locus_tag="ACG8OA_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4976229.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="ACG8OA_001437"
     CDS             complement(14109..16307)
                     /locus_tag="ACG8OA_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4976230.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="ACG8OA_001438"
     CDS             16539..17531
                     /locus_tag="ACG8OA_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4976231.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="ACG8OA_001439"
     CDS             17591..19012
                     /locus_tag="ACG8OA_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFS4976232.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="ACG8OA_001440"
     CDS             19005..20504
                     /locus_tag="ACG8OA_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFS4976233.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="ACG8OA_001441"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="ACG8OA_001441"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS4976234.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6670 a         4494 c         3823 g         7041 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gaccaaat
//
LOCUS       DAZULO010000038        21988 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000038 DAZULO010000000
VERSION     DAZULO010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.039"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..395
                     /locus_tag="ACG8OA_001442"
     CDS             <1..395
                     /locus_tag="ACG8OA_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS4976235.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="ACG8OA_001443"
     CDS             398..1147
                     /locus_tag="ACG8OA_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4976236.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="ACG8OA_001444"
     CDS             1159..1428
                     /locus_tag="ACG8OA_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976237.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="ACG8OA_001445"
     CDS             1433..1597
                     /locus_tag="ACG8OA_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976238.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="ACG8OA_001446"
     CDS             1590..1892
                     /locus_tag="ACG8OA_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976239.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="ACG8OA_001447"
     CDS             1889..2050
                     /locus_tag="ACG8OA_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS4976240.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="ACG8OA_001448"
     CDS             2047..2352
                     /locus_tag="ACG8OA_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976241.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="ACG8OA_001449"
     CDS             2352..2708
                     /locus_tag="ACG8OA_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFS4976242.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2668..2913
                     /locus_tag="ACG8OA_001450"
     CDS             2668..2913
                     /locus_tag="ACG8OA_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976243.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="ACG8OA_001451"
     CDS             2910..3329
                     /locus_tag="ACG8OA_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFS4976244.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="ACG8OA_001452"
     CDS             3326..3883
                     /locus_tag="ACG8OA_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFS4976245.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="ACG8OA_001453"
     CDS             3880..4176
                     /locus_tag="ACG8OA_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976246.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="ACG8OA_001454"
     CDS             4253..4666
                     /locus_tag="ACG8OA_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFS4976247.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="ACG8OA_001455"
     CDS             5012..5386
                     /locus_tag="ACG8OA_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976248.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="ACG8OA_001456"
     CDS             5423..5590
                     /locus_tag="ACG8OA_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976249.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="ACG8OA_001457"
     CDS             5616..5960
                     /locus_tag="ACG8OA_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFS4976250.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="ACG8OA_001458"
     CDS             5965..6246
                     /locus_tag="ACG8OA_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976251.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="ACG8OA_001459"
     CDS             6349..6663
                     /locus_tag="ACG8OA_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFS4976252.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="ACG8OA_001460"
     CDS             6641..8335
                     /locus_tag="ACG8OA_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS4976253.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="ACG8OA_001461"
     CDS             8355..9533
                     /locus_tag="ACG8OA_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4976254.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="ACG8OA_001462"
     CDS             9496..10182
                     /locus_tag="ACG8OA_001462"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFS4976255.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="ACG8OA_001463"
     CDS             10182..11342
                     /locus_tag="ACG8OA_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS4976256.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="ACG8OA_001464"
     CDS             11352..12227
                     /locus_tag="ACG8OA_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976257.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="ACG8OA_001465"
     CDS             12224..12535
                     /locus_tag="ACG8OA_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976258.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="ACG8OA_001466"
     CDS             12525..12878
                     /locus_tag="ACG8OA_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS4976259.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="ACG8OA_001467"
     CDS             12868..13269
                     /locus_tag="ACG8OA_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976260.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="ACG8OA_001468"
     CDS             13262..13666
                     /locus_tag="ACG8OA_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976261.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="ACG8OA_001469"
     CDS             13678..14286
                     /locus_tag="ACG8OA_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4976262.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="ACG8OA_001470"
     CDS             14306..14668
                     /locus_tag="ACG8OA_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976263.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="ACG8OA_001471"
     CDS             14671..14853
                     /locus_tag="ACG8OA_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976264.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="ACG8OA_001472"
     CDS             14870..18289
                     /locus_tag="ACG8OA_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4976265.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="ACG8OA_001473"
     CDS             18340..19077
                     /locus_tag="ACG8OA_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4976266.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="ACG8OA_001474"
     CDS             19087..21378
                     /locus_tag="ACG8OA_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFS4976267.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="ACG8OA_001475"
     CDS             21402..>21988
                     /locus_tag="ACG8OA_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFS4976268.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3245 c         4705 g         6105 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatttg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DAZULO010000039        21912 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000039 DAZULO010000000
VERSION     DAZULO010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.040"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACG8OA_001476"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACG8OA_001476"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFS4976269.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACG8OA_001477"
     CDS             complement(1172..1867)
                     /locus_tag="ACG8OA_001477"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFS4976270.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACG8OA_001478"
     CDS             complement(1880..2596)
                     /locus_tag="ACG8OA_001478"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFS4976271.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="ACG8OA_001479"
     CDS             2926..3534
                     /locus_tag="ACG8OA_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFS4976272.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="ACG8OA_001480"
     CDS             3859..4182
                     /locus_tag="ACG8OA_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFS4976273.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="ACG8OA_001481"
     CDS             4364..5734
                     /locus_tag="ACG8OA_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFS4976274.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="ACG8OA_001482"
     CDS             complement(5864..7165)
                     /locus_tag="ACG8OA_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS4976275.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="ACG8OA_001483"
     CDS             complement(7519..9510)
                     /locus_tag="ACG8OA_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFS4976276.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="ACG8OA_001484"
     CDS             complement(9654..9920)
                     /locus_tag="ACG8OA_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4976277.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACG8OA_001485"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACG8OA_001485"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFS4976278.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACG8OA_001486"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACG8OA_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFS4976279.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /gene="def"
                     /locus_tag="ACG8OA_001487"
     CDS             13264..13827
                     /gene="def"
                     /locus_tag="ACG8OA_001487"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS4976280.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="ACG8OA_001488"
     CDS             complement(14080..14601)
                     /locus_tag="ACG8OA_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFS4976281.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="ACG8OA_001489"
     CDS             complement(14604..15446)
                     /locus_tag="ACG8OA_001489"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFS4976282.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="ACG8OA_001490"
     CDS             complement(15439..16164)
                     /locus_tag="ACG8OA_001490"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFS4976283.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="ACG8OA_001491"
     CDS             16340..16636
                     /locus_tag="ACG8OA_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976284.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16892..17066
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17204..18796
                     /locus_tag="ACG8OA_001492"
     CDS             17204..18796
                     /locus_tag="ACG8OA_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4976285.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="ACG8OA_001493"
     CDS             18809..19549
                     /locus_tag="ACG8OA_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4976286.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="ACG8OA_001494"
     CDS             19717..21066
                     /locus_tag="ACG8OA_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4976287.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="ACG8OA_001495"
     CDS             complement(21194..21784)
                     /locus_tag="ACG8OA_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS4976288.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6295 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aa
//
LOCUS       DAZULO010000040        21831 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000040 DAZULO010000000
VERSION     DAZULO010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.041"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACG8OA_001496"
     CDS             133..3660
                     /locus_tag="ACG8OA_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFS4976289.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACG8OA_001497"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACG8OA_001497"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFS4976290.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACG8OA_001498"
     CDS             7448..7789
                     /locus_tag="ACG8OA_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS4976291.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8OA_001499"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8OA_001499"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFS4976292.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8OA_001500"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8OA_001500"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFS4976293.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8OA_001501"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8OA_001501"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFS4976294.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACG8OA_001502"
     CDS             13000..13737
                     /locus_tag="ACG8OA_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4976295.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACG8OA_001503"
     CDS             13751..14566
                     /locus_tag="ACG8OA_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4976296.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACG8OA_001504"
     CDS             14579..15226
                     /locus_tag="ACG8OA_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4976297.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACG8OA_001505"
     CDS             15242..15898
                     /locus_tag="ACG8OA_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4976298.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACG8OA_001506"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACG8OA_001506"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFS4976299.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACG8OA_001507"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACG8OA_001507"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFS4976300.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACG8OA_001508"
     CDS             18240..18758
                     /locus_tag="ACG8OA_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4976301.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG8OA_001509"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG8OA_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFS4976302.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="ACG8OA_001510"
     CDS             complement(19645..20565)
                     /locus_tag="ACG8OA_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFS4976303.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20691..21743
                     /locus_tag="ACG8OA_001511"
     CDS             20691..21743
                     /locus_tag="ACG8OA_001511"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFS4976304.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
BASE COUNT         7315 a         3774 c         4665 g         6077 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta c
//
LOCUS       DAZULO010000041        21726 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000041 DAZULO010000000
VERSION     DAZULO010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.042"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(205..1104)
                     /locus_tag="ACG8OA_001512"
     CDS             complement(205..1104)
                     /locus_tag="ACG8OA_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4976305.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1114..1470)
                     /locus_tag="ACG8OA_001513"
     CDS             complement(1114..1470)
                     /locus_tag="ACG8OA_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS4976306.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1825..2121
                     /locus_tag="ACG8OA_001514"
     CDS             1825..2121
                     /locus_tag="ACG8OA_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFS4976307.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2765..3718)
                     /locus_tag="ACG8OA_001515"
     CDS             complement(2765..3718)
                     /locus_tag="ACG8OA_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4976308.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3741..4496)
                     /locus_tag="ACG8OA_001516"
     CDS             complement(3741..4496)
                     /locus_tag="ACG8OA_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4976309.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4493..5452)
                     /locus_tag="ACG8OA_001517"
     CDS             complement(4493..5452)
                     /locus_tag="ACG8OA_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS4976310.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5445..6395)
                     /locus_tag="ACG8OA_001518"
     CDS             complement(5445..6395)
                     /locus_tag="ACG8OA_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS4976311.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6545..6997
                     /locus_tag="ACG8OA_001519"
     CDS             6545..6997
                     /locus_tag="ACG8OA_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFS4976312.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="ACG8OA_001520"
     CDS             complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="ACG8OA_001520"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFS4976313.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="ACG8OA_001521"
     CDS             complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="ACG8OA_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFS4976314.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10549..11358)
                     /locus_tag="ACG8OA_001522"
     CDS             complement(10549..11358)
                     /locus_tag="ACG8OA_001522"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4976315.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11367..14948)
                     /gene="smc"
                     /locus_tag="ACG8OA_001523"
     CDS             complement(11367..14948)
                     /gene="smc"
                     /locus_tag="ACG8OA_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFS4976316.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14968..15654)
                     /gene="rnc"
                     /locus_tag="ACG8OA_001524"
     CDS             complement(14968..15654)
                     /gene="rnc"
                     /locus_tag="ACG8OA_001524"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFS4976317.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(16002..17783)
                     /locus_tag="ACG8OA_001525"
     CDS             complement(16002..17783)
                     /locus_tag="ACG8OA_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4976318.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17809..18723)
                     /locus_tag="ACG8OA_001526"
     CDS             complement(17809..18723)
                     /locus_tag="ACG8OA_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4976319.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18750..19712)
                     /gene="opp4B"
                     /locus_tag="ACG8OA_001527"
     CDS             complement(18750..19712)
                     /gene="opp4B"
                     /locus_tag="ACG8OA_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS4976320.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19712..20656)
                     /locus_tag="ACG8OA_001528"
     CDS             complement(19712..20656)
                     /locus_tag="ACG8OA_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4976321.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20657..21661)
                     /locus_tag="ACG8OA_001529"
     CDS             complement(20657..21661)
                     /locus_tag="ACG8OA_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4976322.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         6069 a         4638 c         3846 g         7173 t
ORIGIN      
        1 tatatttttt tttgtgatta tcgttcaaaa aaagaataca cgcttgcatc tagtactctg
       61 cgagttacaa atcatccgat caaataaaca ttcgctgatt attttccgca aaaaaagcag
      121 catatccacg ataaatggat ctactgctct tacttatcct tatgttttac aaggtatttt
      181 cttttacaca ttactaatgc tgtctcaata cggaacgatt gtcatcgctt gttcaaattc
      241 ctcttgcaaa tgttgtgttt gataggcctg aaaagctggg tgatgggcac gcaagaggac
      301 acgtaatttc ataggatcat agctgtctag caactgttct ttcaagacaa aatctcgggt
      361 cagctgccat tcataataat aaccaaaagg tctgcagctg taaatcgtgt gaccgatgga
      421 tttatcttca aagcgacgat gcgtatggcc aaaaactact tgtttgatat ttgaataacg
      481 gtcaagtaat tctccgaatg attctgatcc taaaaatgca tttaatttgt tccaacgctc
      541 ataaggagca gattgataca tgataaatgc ccgtttagga acgaaatgag tggataaaat
      601 cacctcaagt ttttttgctt ccagctggtc tagcaaattt tttgcagctg caagcatttc
      661 tgcattgacc tcgagatctg ttttcccacg ctggatgaac cgatcatacc aatacaaacg
      721 cttcattcgt acgattgcct cgtaatcttt caattcggaa aactgatagt cgtaccagcc
      781 attcatccct aaaagaacgg tctgctcatt taaagcaata taccgttcat ttaaaaaatg
      841 tgggtctgga aaatggtcca tcatctgttc attgggcaca tctgccaact cgtgattgcc
      901 gaaatgaaat gtcgtagata atcctttatt gacgaaaaat tcgacaacag ctaggcaacg
      961 gtctacttta ttagccgtat ctccggctag atgaagccga gtgatcttgt gcttttgcaa
     1021 aacttgccaa agctgcatca gctctgattc gccgaattta ttgatgtcca catgtaggtc
     1081 gctgatgatc gccaattttc ccattttatc cctctagtct tttcgaacaa cttgcaagtt
     1141 gccttttctc aagccgcttg gtccttttaa ttgtaaacta ttataagttg gttctaaagc
     1201 aagtgtggat ggatcatcca tgtaaagtat tgcactgtct ttgattttga ttggtccaac
     1261 tggtgtatca gccactacag aataaagact tagatctgtt tgtgtatctt ttttgacaat
     1321 gatcaatcga aatgccgtat ccaattggca actgacttgg ctttctgcaa atggaccgtc
     1381 accgttttcg atatctaata aaagttgatc gtctgcttct aatttattct tcaatacttc
     1441 ttgtgcttct tttgtcactt ctaatttcat ctgtatcacc tcatgtcata gaataatcac
     1501 tttattcatc agatgcaact aatttgtctg gttctgactg ttctcaattt attggaaagt
     1561 aagtcattcg tttattatga tttctcttat cacaaaagag acaactttcc cttttcttcg
     1621 ttcagtttct tgaacggaaa aagacccaaa aagcaacctg ttatttgttt aaccttattc
     1681 ctttcttaaa taacaaaaaa tactataaaa aataaaaaac ctttaacaac tattggaaaa
     1741 agaattactt tttgttcacg gattattaac aactcttcgg tagagtaaac ttatcaaata
     1801 aaagacttct ttggaggaat cactatgaag gaatatataa aaaacatcta ttttatcgaa
     1861 gaaacacaaa atatcgaggg ttcttatatt gaagtaaaaa cactctttgt caatgaagat
     1921 aaaaccaaag cattggatat ttataaaaaa ttagcttcaa aaaaaacgaa tagttttgga
     1981 ttgatattaa gcgagtataa aatcaaagca gaagagagtt atttctatca attattaaaa
     2041 cggtggagta agcttcccgc cgatttttat cgaaaaatgc agatcatcaa ctatcaaccg
     2101 cttgctgaga cacatgccta aatagaggtt gtgaaatcag tgctttgagc taataccgaa
     2161 ggtcagctga ttgaacattg tttagaagag attgtgacaa ctgcgatttg ctttgagtaa
     2221 taagaacgct ggacagaaaa tagcttctca tattttctag tctagtgggc ttattgccga
     2281 gaagcagcag atggaacatc gtttagaaga gattgtgaca actgcgattt gctttgagta
     2341 ataagaacgc tggacagaaa atagcttctc atattttcta gtctagtgga cttattgccg
     2401 agaagcagca gatggaacat cgtttaaaga agagatcggt acaaaataaa aatatctaaa
     2461 tttaagtttt atctaagttt cgctttgtac ctttgattca aatcaaagga ggcgatcttg
     2521 atgctgaaaa aatggatcac actatttttt gccactcccc attctagaat caacccgtat
     2581 gatgtagata atgaattcac gttgaaaaaa gatttctcaa ataaataaaa accatccgta
     2641 aaatcatcaa tacgatttta cggatggttt ttgataactt ctttgaggat aggctcttgc
     2701 tatttaagaa tgtaagaaac attattgtta catagtatga gagtttaatc ctctgttatt
     2761 tatgctattt aaatgcttga ttcacatctt cgatcatcag tttcattgat tctaaaccgc
     2821 ccccgcttaa ataccagaca tctggttcaa gggagatgat ctgctgattt ttaccagcat
     2881 tcgtttgtgc taccagttca ttggcagaaa tatcatcttt gctgtcgtct ccaccaatag
     2941 cttttgttct gtctactaca aataagatat ccggattttt ttccagcaca tattcataag
     3001 aaacactttg accgtgagtg gatgcttcga tttgatcatc tgcttgttca aagccaaata
     3061 aatcatggat gaaaccgaat ctagaaccag atccgtaggc tgataattgc ccttcattca
     3121 caagtgtgac tagtgctttt ttgtcagtag cttctgcttt ctttttcgtc tgatcgattg
     3181 cactagataa ttcctctaat tgagttttag cttcttcttg cttaccaaag atctccccta
     3241 aagcagtgac attttgctgg aaagattccc atggattttt cgcatctaaa gaaaggaaaa
     3301 tggtcggagc aatttgactt agatcttttt gaaaatctct ttgtcggcca gaaatgatga
     3361 ttagatctgg ctgaagctga ttaatttttt ctaagtctgg ttcttttatt ccacctgctg
     3421 attctacttt tttataggat gaaagatact caggaaggct gcttgtagct gctccaacta
     3481 ccttatctcc aacaccaaga gcatccatcg tatctaaaga accattgtcg aatacgacga
     3541 ctttttctgg attatttggc acctcgattt ggtctccatt actatctgtt atcgtcaatg
     3601 tagtttcagt ttgtgtgctg cttttttttg tgtctgcctg actggtgttg ttgctatttc
     3661 cacatgctgc taaaccaaat gatgctagaa tcaagatacc tactactaat tttttcatct
     3721 cttctctcct aatgttagtt ttagaaataa agacagaatc tgcggccttc aatctcacaa
     3781 atccgtatgt ccatttcgta taattcatcc aatacatctt tttttatcat ttcttgggtc
     3841 gttccttgcg taaataactg tccatctttc attgcaacaa tttcgtctgc ataacttgca
     3901 gcaaagttga tatcatgaag aacaataaca attgttttcc cgatttcttg tactaaattt
     3961 tttaaaatct gcatgatttg aaccgcaaaa ttcatatcta gattgttcaa aggttcatcc
     4021 agtaaaatat aatccgtgtc ttgcgctagc accattgcaa tatacgcacg ttggagttga
     4081 ccgcctgaaa gagtgtcaat cgattgatcc gctatttctt ctaacccaag gtgttccagt
     4141 gattcctgaa cgatttcctc gtcttttgtc gtcaattttc ctttcgtata aggaaatcgt
     4201 ccaaattcta ctaattcttt cacggtgatt tggacattta tttcattcga ttgtcgtagg
     4261 atcgataatt ttttggctaa ttcattggat ttccaagcct ttacttcgtt atggtctaaa
     4321 tagatttcac ctgtatcttt tggtatcaaa cgactgatca tcgacaacac cgttgacttt
     4381 ccagcaccat ttggaccgat aaaagcagtc aatttctttt caggaatcgt taactgtaca
     4441 tctttaacca ctgattttcc atggtacagt ttattgacct tttgtaaatc aatcatagga
     4501 tactcttcct ttcgcgaatc aattttgtga caaaataaat tcctccgaca aattcaacga
     4561 cgacactcac tgttgtattc cagccgaaaa tctgctctac tgcgagctgt cctcctgtca
     4621 gaaacagcat agctaccaga aagcttccga taaatagctg accatgacga taagtagaaa
     4681 agacacgata actgatattt gcgacaataa accctaaaaa agtaactgga cccacaagag
     4741 ccgttgatgt ccctgtcaaa agactgacac aacataaaat caaaaattca aaacgatctg
     4801 tgtctatccc gaggtttact gcttgttcat ttccgagatg gaaaacgtct agcacatgtc
     4861 gatatcgaaa taacaggtac cagcagccga taatgatcaa agaagcaggc aaaagatact
     4921 gactctggat attgccaaag cttgcaaata atctgccttg aagcaaatca tattcattcg
     4981 gatccatgat gacttgtaaa aaggtactga cactaccaaa aagcgtacct aaaatcatcc
     5041 ctgacatcag taataaaaaa agattcgctc tcattttttt caaaagaaag aaaaataaca
     5101 atagactcag cccgaccatc agcccaacgt tcaataagaa aaaaactgta cctgtctgct
     5161 gaagcatcgt cactcctccg ataaagaaga aaaggaccgt ttggacaagg acatataacg
     5221 actcaagtcc caatactgaa ggagtcaaaa actggttatg cgtaattgtc tgaaagctga
     5281 ttgtggcact gctgacagct agcgagacaa ggataaatgc aaaaattttc ttactcctca
     5341 atgcacatgc aaaatgccag tttccatatg tatggatcgt caagtacatt cctgtaacaa
     5401 ataaaacaag aacaagcata ccaataaaaa tagatggctt ttttttaagc atatcgtcgt
     5461 ccccttatca gaagataaag gaagatagcc ccaccgatga ttccaacgat cacactgact
     5521 ggtatttcat aaggaaaaat caacacgcga gcgacaagat cgcaaaatac taagaacaaa
     5581 ctacctgcta aagcagttac gccaattgtt ttgtgcactt gatctccata tattctagag
     5641 acaagattcg gaatgatcac tccgataaaa ggaatgcttc ctaccatgat caacatgact
     5701 gcactagcca atgatactaa gccaatgcct aaaaactgca tcttctggta atctattccc
     5761 agatttactg caaacgattc gcccagacca atgatcgtaa atcggtaagc aaatagccaa
     5821 accaagagca acaatggaac aacagcatac aataactcat aatttcctcg tatcacagtc
     5881 gcaaaattcc cttgaagcca tgaagtcata ttttgtacca actggaattg ataagcaaaa
     5941 aaagtagcaa ctgaaccaat gatattccct aacatgactc caatcaacgg aagcatcaat
     6001 tgatttttct ggaggaacaa tcgcgtaagg gataaaaaaa gcaatgttcc tgcaaaagca
     6061 aagcaaaatg ctgtcaagga tcggacaatc aaggatgcac caggtaggaa aatcatagcc
     6121 actaaaatac ctaaacgcgc actatccatc gtgcccgcag tcgttggtga aacaaattta
     6181 ttttgggtca aatgttgcat gatcaaccca caaatactac tggtcgctcc cacaaggatc
     6241 aaacaaatcg ttcgtggcaa acgagtcact agcaagattt gtaagctttg cccatctaac
     6301 tggagcagtt ctgacaattg tacattcgat actccgatga acaatgaaat actacttaaa
     6361 ataactaata atgctactag tcgtccgtag cgcatccaat ccacctttct aattgagaat
     6421 gattttcatt cttattttaa gtgaatcgtt gaacaaaagc aaccgctttt tcatatgttt
     6481 taggtattat tgggaatatg ctataatcgt aagaaagaca aagagaagaa ggagggtatt
     6541 ttttatggca caagatgatg taaaaagcca tttggataag ggactttatg gtacaccttt
     6601 agtgaaccct gaagaacagc ataaatattt agggacattc agggaacgct gctatcttag
     6661 tatgactgtt gctgaaatga tccaaccaaa aaacaaagaa aactttttaa aagaactcaa
     6721 aaaacatcca gatgcttcca tactcttgaa tggggctcta cccctttctg tccaaaacca
     6781 atatatccag ctagcaacga aaaacaatag ccgtttcact gtagtcaacg attacgtcac
     6841 tgcaaatcct gaggaattcg gattgctctt aacagcaaaa gaagcagtaa atgaacctgt
     6901 gattgatatc gaaaaaaaat acccagaaga aaactcttcg ccagctgaaa aagaaaaacc
     6961 aaaaaagaaa aaaagcttct gggatcgttt attttaaaac gaaaaaacac ataaattttt
     7021 acatgcaaaa aaagcttttg atcagattga catcaaatag gacaatctgt caaaagcttt
     7081 ttcttttaaa atacttcagg atataacaat ttcaatacat tcatcgtcaa tcttctgcct
     7141 tttcctgcaa aaggataaac atgcatatgc atggctggat tgaaatttgc ttcgatgatc
     7201 ccatatgctt ttttatcagt tgtaggatcg atttctttat ctggtataat caaatcgata
     7261 ccactaatct tagctcctag tgcttctacc gcagaaacgg cgattttttt gtaagcttca
     7321 gaaaattcat cagtcatatc gatcgaatca ccacctgtac tgatatttga gttctccctt
     7381 agataaacaa tttgattggc ttggggaact gattcgatcg tcaacccttg ttctttcaac
     7441 atcaattgtt ctaattcacc taattggatc aattctaaag gtgcacgatg attcgttcca
     7501 cgcaaagggt cactattttt ttcttcaaca agttctttca ctgttcggat actgtcacca
     7561 ataacattag cagggactcg caacatgatg gcttggactt ctccgtcgat cacaaagaaa
     7621 cgatattccg ttcccggcat aaattcttct actaaaaccg aactgtcttc acgaaaagca
     7681 atggctaaac ctgcttgata atcttccaga ctagctcctt ccttgaaaat cgtgatacct
     7741 aacccgtaat tggttgattt aggtttgatc acaaatgcct gctcggcaaa tctcggataa
     7801 gcttttaccg cttcttccat ggatgaaaat tctgcgcctc ctggtacccg gaacccagct
     7861 tcttttaaga cttttttcgt tactgttttg ttttccatga taagtgggac gatatagctg
     7921 tccttacttg tcatgtttgc atttttcaca tattctacat ggtcttggaa ttgcagacgc
     7981 aaaaattgat ccgactcatc taatactttg acttggacac ctttttggat ggcatcgaac
     8041 ataaatatct gagtagaaag ttccatttcg cgaaaaccgg ccaactgata gggacgttca
     8101 tggctttctt tataatattg atgtcccaat gccacagcaa cttcttgctg actcgatagc
     8161 tgtgcttctt gatagagttt cgctgctaac gtttgttcag gatgatccat ttgagtcaat
     8221 gcttcctcta ctaaataaag agaatccaac caatccaatt caaccagcat ttgcttcatt
     8281 ccttcaagaa tttctctacc ttcttgatga aattctgttt gatctgatgg ttgttctagt
     8341 gctactttgt tatttcgagt cgtaccttct gctacccatt ggtcacagtc ttctttttct
     8401 tctgtccata acatgtacat caggaataat tgtaagaacc gaacttgctc tggattgata
     8461 cccaatcttg cataaggatt caaatcgata ttccgcaatt caatataacg aacacctgtt
     8521 gttgctaaat ctgcgacttt ctttccgcct cggaaacgaa gcggtgtata aaattctttc
     8581 tcctccgaaa gtttcccttc ttcgatcatt cgctcgatat ctgctaaata ctgtttcata
     8641 gaagcatagg aaactttcac atttggatga ttcgtatagc ccagcgcact gttgcgaata
     8701 ctgcgaacag gttcttgcgg atgtgattca tctaaaaagt agttcttctc actcattggt
     8761 gaagcaccga ataaataagt gatcatccag cgatagcgca taaagtttct cgctgttttc
     8821 aaataaagtt ctgttttgaa ttcggaaaaa tcttgaaatt cttcctgatg gctgaacaat
     8881 gttcttagca actcatcgcc aaattcaaaa ttgaaatgaa taccgcttac catctgtttt
     8941 cgcttcccgt attcttttgc taaataacgt cgatacaaga catcttcgaa attttttaat
     9001 tttgcaataa tgatctcttc gtctttttcc ggtaaggcag gtggcatact taatggccag
     9061 atcatctctt ctttcggtat agaacgagca gtcacatcat aaacagcagc gagatactga
     9121 aataattcgg gaatagaatc ggtaacaggg gtgatcatct ctgtttgtgt ttcgctaaaa
     9181 tctgtttgga tataggggtg ataggatcga tcgccaaaga ttgctggatg atcggttttt
     9241 gctagatttc ctgcaagatc aacccgctgt ccttcccgtt ctataccaaa tcgagcttgg
     9301 tcgataaaag ggcgcgcatt gacatgcaat aataattgct taaaattcat cattaagtga
     9361 cctccaattc atatgaatct aattataatc aaaaagtcta agaaaaagaa cgaaattgtt
     9421 tgagaaccgc ttacaaataa gtataaaaat gaaggcatca aaaacacagc tatgttaaaa
     9481 acaatcattc aaacaaggag ctgcaacttt tgtcacagct cctgcttttt agacatgggt
     9541 tattcttctt ttagtaatcc tttgaacaat ccaacgacaa aatcatttgg atcaaacggc
     9601 tccaagtcat cgattccttc accaagaccg accaatttca ctggtaaatg caattcatta
     9661 cggatagcaa gtacgatacc gccctttgct gtaccatcta attttgtcaa aacaagaccg
     9721 gtcacatctg ttgtttcttt gaattgtttt gcttgaacca tcgcgttttg accagtggtt
     9781 gcatctaata caagaagtgt ttcttggggt gcttctggta attcccgctg gattactcgt
     9841 tttatttttt ctaattcgtt catcaagttc actttatttt gcagtcggcc tgcagtatca
     9901 actagtaaaa catctgcctg ctctgtcttt gctctttcca aagcatcgaa tacgacagct
     9961 gctggatcgc ctcctgcatt gccacgaaca acttcaacac cagcgcgttc gccccagaca
    10021 actaactggt cgattgctcc agcgcggaag gtatctgctg ctgccatcaa tactttttta
    10081 ccttcgtttt tgaactgatg ggcaagttta ccaatacttg ttgttttccc tacaccatta
    10141 acaccgacaa acaaaataac tgttagtccg tcttcttgga tatttagttc atttacttca
    10201 ttgattcctt ccgcttcata gagatccaca agtttctcga tgatcgcatt ttggacttct
    10261 gccgattttt tcgcatttct gagtttcact tcttgacgta atgcttcggt aattcgtatc
    10321 gctgtttcaa aacctacatc tgcaccgata agtgtttctt ctaattcttc aaaaaagtct
    10381 tcatcgactg ttcgaaaatt ggcaaacaac tcattcatcc gttcaccaaa tgttttacgc
    10441 gtcttttcta aacccttgtc gtacttctcc tgtacttcaa cttcttctgt tttctcttcg
    10501 ccagtaaaag cacgcttgat tttatcaaaa aatcccattt gactccccct actctaatac
    10561 aaatttttgg atcactttgg ctacgccatt ttcttcattg ctggctgtca cgaaatctgc
    10621 tgcttctttg acgaaaggca cagcattctc catcgctacg cctagtccag catattggat
    10681 catcggtaaa tcattttcct catcaccgat cgccatgatt tcttcttgtt tcaaaccaag
    10741 atcttttgcc aataaagaaa taccataagc ttttgtaaca ccttttggca taaattccaa
    10801 aagcatactt cttgttttga tgatctcata catttcatgg aattctgctg gtatttcttt
    10861 gattttagga tctaattctt cttggtcata agcgattact gccttattaa gcacaaattc
    10921 atctgtaata tcgcttatat ccgctggttg gaattgcagc agcttattca acacattgta
    10981 caaggattgc ttccttgggg cactaggcag ctgtaaaacc acattgtcag acaaaacatc
    11041 tagtggcaga tccaattgct gagccagttt gtataagcgt tggatatctg atacttccat
    11101 cactttcttt tccatgattt caccagtatc atttttttgt accaaaccgc cgttgaaagt
    11161 gatgctgtag tcaccttcat ctaccagtcc taattcttgc agataatgcg ccatcgcagc
    11221 taaggggcgt cctgtacata gaacgatctt gaccccttgc tgttttgctt gtgctaatgc
    11281 ttgtttattc tcatctgaaa tcagcttttg ttcatttaat aatgtaccgt ccaaatcgat
    11341 tgctactaat ttgatcattt tcgttcctac actttcgttt cgttgatttt cgcctcttca
    11401 ttgatatctt ccatccgtac agaaacgatt tttgatacac cggattcctg catcgtcact
    11461 ccgtacagta catctgctgc gaccatcgtt cctttgcggt gtgtgactac gataaattgt
    11521 gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt tcacgttggc ttcatctaac
    11581 gctgcttcta cctcatctaa gatacaaaac ggaacaggac agacacggat aatggaaaag
    11641 agcaacgcga tggctgtaag tgctcgttct cctccagaaa gcaaacttag actttgcagt
    11701 tttttgccag gaggttgtac ttcaatctca atacctgttt tcagcaaatc agttggttcc
    11761 gtcagtacta attcagcttt accgccgcca aacatatttg gaaagacaat tttgaattct
    11821 tgacggatag cttcaaatac ttctttgaat cttgtccgca cttcatcatc catctcatcc
    11881 atcgtttcaa acaattggtt tttcgctgct aacagatcat ctcgctgtgt tgctaagaat
    11941 gtatgtcttt cactgacttg ttcgtattgt tcgattgaat ttaagtttac tggtcccagt
    12001 ttttcaatct gttcttttaa ggatgaaacc tttgttctac tgtcttcgat atcggttgtt
    12061 tcttgatagt cttgggaagc tttctcaaat gtcagctgat attctgactg cagataggtc
    12121 aaatgattat ctaaaatcat ttcagctcgg tctttttgga cttctaattt ggattgttcc
    12181 gacagtcgtg ctttttgctc acgattacgc tctgccaaga ctgcttcaag cttatcgatc
    12241 tccttttgcc ttctatctcg ctgctctttg gcttttgcca actcggcttt taatgtttct
    12301 cgctgagcag ataattcgtt aatttgtttt tccaaacttt cttctgtgac ttcatgatca
    12361 gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg cttcttgacg ttcggttgcc
    12421 tctgcttttt ggactcgggc accgcggagc tggatctgca gatgattaaa ctgctctttc
    12481 aagacagcaa gatctgcttg ttcttgtgct ttttgacttt gtacttgtgc tcttctggct
    12541 tccatctggt cgctttcttg acttaacgat ttgatttctt cgtcgatttt ttgtcgttgc
    12601 gattcaagat ctgtttgttt gtctttcaat tcgttcgttt gcttttcgta atcttcgata
    12661 aactgctgca gttcacgtgt ctcaaaagtt gagatttgct tttctttttc aaaacgttca
    12721 agttcattta tgatgttttg tagctgattt gtcgcttctt gttcttcaaa acgtaattgt
    12781 tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac gagctgtttc ttgttgcagc
    12841 tcctgaactt ttttttcttg tgcttggagt tgtttatctg cttcttcaaa ttcactagtc
    12901 aactgtttca attcttgatt ttgtacaaac aaactgcctt ggtttcctcg tttattcgct
    12961 ccacctgtca tggatcctcc ggcattcatc acatcacctt ctaaggaaac cactcgatat
    13021 tgatagcgga tcgtttgcgc gattgcattg gcactagtta agtctttggc taaaaggatc
    13081 gttcctagca ggttttgaac cacagtttgg atttgatcgg gataactgac ttgttcactg
    13141 gcaatcccta taaacccttc tacagctgct gcttgtgtca atatatgagc cggtaattgt
    13201 cttggtttga ttgtcgtcag tggaaggaat gttgctcttc ctcctctttg ctgtttcaaa
    13261 tacgtgatgg cttgccgagc atctttttca ttttcaacga tgacatgctg ggctgcccca
    13321 cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa catcgatcag ttcagccact
    13381 gctccgacaa tacccgaaag ctgctgttta tgctgcaaga ccaatcgaac cccttggtaa
    13441 aaaccaaaat agttttcttg gatttcttga aggcttttct tacgagcacg tagctgctgg
    13501 acttgattca ttaattgata cattttaggc tgttcttttg ctaatgcttc ttggaacttt
    13561 ttcccgtttt gttggatcat ttctaacttc tcttttgttt cagttaaaga tgattttagc
    13621 tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca atcggtctac tgatgcttct
    13681 acttccgatt gcttggctaa cgtctgcttg ctttttgctg tttcttgtat atattgacgt
    13741 tctagatact tcagttcgtt tcctacagct gcttgttctt gcatcaaatc aacatattga
    13801 tctcttaatt ctgccaataa ttctttcgtt gatttttggt atttttctaa ttcttgctgt
    13861 gtttttatga gatttgcttc agctttttgg atctctgttt ctttttctgc tgctgctttc
    13921 atcaagcttt cttgaagctt ttcaaaatgt ttaacctttt tttgtgcttc tgctaatgaa
    13981 gtttgatatt cttgcgagct tttttgtgta tgctttgtcc gctcctgcaa aacatctttt
    14041 tgtccttctg tctgcttcag tttttccgaa agatctaata atacttgctg atttttttcg
    14101 atcagacgat cggcttgcgc attttcttta cgctgcttcg ccaaaatgga ttcttgttct
    14161 tggatcgact cagatagttt gcctaattct agattgaatt tcgcaagctg tgcttgtttg
    14221 ttgtcccaat ctttttttgc tgtcttgatt tcagcaacca tcaaagagac atcggtttga
    14281 gtcagtgttt cttttagccg taaaaactcc tttgctgcct cactctgggc ggccaaagga
    14341 gttaactgtt cttccaattc gtggatgatg tcttggacgc ggctgaggtt gtcttctgtt
    14401 tcaaaaagct tctgttctgc tttctttttc cgctgcttgt atttcaagac accagctgct
    14461 tcttcaaaga tcccacgacg gtcttccggt ttactactga aaatcgcttc tacttttcct
    14521 tgagaaatga ttgagaacga ttcttttcct aatccagaat ctaagaacaa ttcttggata
    14581 tcttttaatc tgcagctttg tttattgatg aaaaactcgc tttccccagt ccgtcgataa
    14641 cggcgggtta cactgatctc attgtactcc aatggcagat aatgatcact attgtctaaa
    14701 atcactgtga cttctgcaat attcagctgc ttacgggtat cggaaccggc aaagatgata
    14761 tccggcattt ttccgcctcg cagactttta gcagattgtt ctcctaatac ccagcggata
    14821 gcctctgtaa tattgctttt tccgctgcca tttggtccaa ccactgctgt gacgctattt
    14881 tcaaaatcaa tgactgtctt gtctgcaaat gatttaaaac cggctatttc aattcgtttc
    14941 aaatacaaag ctttccctca cttactttta cagtgctgcc aatgcatttt ccgccgcagc
    15001 ttgttctgct aattttttcg atttcccttg tccagtgcca atcaattgat catctacata
    15061 aacttcgatc caaaatactc gttcatgagc tggaccttct tcattgatca atcggtattc
    15121 gattgataca tcacctgatt tttgcaagac ttcttgtagt tttgttttat gatccatctc
    15181 atgtgaaaaa gcacctgctt tgactttagg aaaaatcact ttttcgatga atgcatgggt
    15241 cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa gcttcgaata ggtcgcaaag
    15301 caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc ttgcccaaca aaacatattg
    15361 atcaaagtga cattctttgg caaatttcga caagctgtct tcgcgaacga tcgcagctcg
    15421 tgtttttgtc aattttcctt ctggcaaatg aggatataaa cggaataaat actgagaaac
    15481 catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc tcattatccg ataattgcag
    15541 attgcgatgc tcattcacat aggatgaatg ggtaaaagct tgttcaagca aatttagatc
    15601 gtgaaaaacg atgttatagt tttcttttag ttcttttgtc agctggttgt ccattttcca
    15661 cattcctttt atggatattt tttcgatact tccttattat actggttttt tgtcaaattt
    15721 gaaatggttc ggtcaaaagg taagtaaagc tttaataatc ctagtgattg acgagaagtt
    15781 gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt tgtgaaaatg gctttccata
    15841 tttttgcata aatttgactt aataccgaga aactggactt tgaacaccgt ttattggaga
    15901 agtcatgaaa tcagcgcctt gacctgaata ttagagaaat aaacagaaaa atatcaaaaa
    15961 accgaaaaag gacatcgccc tttttcggtc atttttagtg attatgaacg tgaatctgct
    16021 gtaacagaaa catttgccca tgggtttggt acatcataag accaatccca gtctttgaca
    16081 cggtcgttga ctggcaatac ttcattacgg aacaacgttg gaatcgcaaa tgcttcttca
    16141 aatgcatatt gttgccattt ttgatatgct tctttttggt actcttcatc aaatgctttg
    16201 cttgaactga tatcgttgat caattttgta ttttcttctg attcataacg agtgtagttg
    16261 aacgcagcat ttggtcccca tagtccactt ggatttggat cagttcctgt attccaagca
    16321 gcttggtaaa catcaatttc aggatcatcg ttttctaatt tatcatagaa agaattgaat
    16381 tcgatcagac gacctgttgt gtattggaca tccaaaccaa tttctttcca ttgttggata
    16441 tagtaatctg ccaatggttg cgctgtttcc ccaccggaca tcgaagcaaa gttgattgtt
    16501 agtttatttc catctttgtc ttctcttaaa ccatcgtcat ccacatcttt gtagccagca
    16561 tcatctaata gttttttcgc tttgtctaga tcttgtgtga atcctttcat ctcatcatca
    16621 tggtatgact ggaatactgg aggaatcaat gaagtcgcat tgcttctcaa acctgcatag
    16681 aaacgttcac ctactgcatt attatctaat gcatagccca ttgcttgacg taatgattta
    16741 tcagccattt ttgaatctgg attgtattcc acactctttg tatctgcatt ccatttacct
    16801 aacttgaatc ctagataagt ataagaagtt tgttcacggc ctaagtttgt atagccagca
    16861 atttctttat aggtatcgta agtatctgtt ggcatttgga aaatcatatc atataattta
    16921 gagttcaatg cttcagttgc acttgccatc ggtacagatt tcatcgtgat ctttttcaat
    16981 ttaggcgtac cattccagta gtgctcgttt ggcaagtatt ctactgattc tcctgcaacg
    17041 atattactca tgtaataagg gccaaaagtc actggatttt tacgtacttg atcgcttgat
    17101 tccatatctt tgattgctac accttctaac gtatgttttg gtgctgcata tgaccagata
    17161 ccaccacccg attgaagcat gcttgggttt acttctttat attcgatttc aactgttttg
    17221 tcgtctattt ttttgattcc ggagattgtg tcggcacttc catcatgata ttcttccata
    17281 cctacgatgt tacggaaagt gtcgtcataa cggataccag tgtaatcttt attcccaatg
    17341 atttcgtaag ggaaaatcac atcgtccgcc gttacatctt caccatctga ccatttgacg
    17401 ttgtctttta ctttgattgt cgcttttttc gtatcttcat ctagatcaag cgttgcagct
    17461 ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata gcggttcatg agaaggagcc
    17521 ataaaagctg catcatatga atcttggtaa aactcccact ggaacaatcc tttaaactga
    17581 ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga ttgcatcatc attatttttt
    17641 gttgcaattg gaaaagttaa ttcctctttg ttattgccgt tgcctccaga gttgtttcca
    17701 gaggtatctg tacctccgcc cccgcatgct gcaagaacga cagcagacat cagcgctgca
    17761 aatccaaaaa tcgttttctt cattcctatt tcccccttcg cttctatact agcctaaccg
    17821 ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca taattgatac tcaacatcaa
    17881 aactaaaata aggattgatg cgggtaacca gatccattgc cgattcacta agacttctcc
    17941 gttacttgca taaccgatca atgtacctaa actaggtaca tttgccggca agccgaaacc
    18001 taagaaagtc aaagttgtct cgataccgat gtttgccgca aagttcatcg tcaaattagt
    18061 aatgattaat gaacttagat tcggcatgat ttcgcggaac atgattttaa aatcattggt
    18121 tcccattgtt tttgaggcac tgacataatc tcttcttgct tcagataatg tcttgcttcg
    18181 gaataatcgg gctttagcta cccaataaaa tgcactcatg atgaatataa aggaccagac
    18241 attgtatctg gaaataatcg atacgaagac gataatgatc agcatgatcg gtagaatcat
    18301 gataaaatca acgatccgca tcaagatgtt gtcaatgatc cctccgtagt agccagagat
    18361 gatccctaat ccaaccccga tgatcgaagt aaggatcgtg atcgcaaatc cgatcgtaac
    18421 agagtttcta gcacctatga tcaattgtcc taatacatca cgtccgcctt catctgctcc
    18481 cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt gcagcgtatt tgtcgaagat
    18541 actaacatac atcactttgt cttgatcggt cagcatcgca ccgataaaga tcactaataa
    18601 taaaaccact agcaaaccta atgaaaaaat agcaatttta tcttttttaa attctcgagc
    18661 aatcattcgt atccccatag gaggaatact ttcttgaact gctgtattgt ttttttcact
    18721 catcgatcca ttctcctttc tgattggttt tattgaatcc gaatacgcgg gtcaacgata
    18781 ctcatgatga tatctgaaag taatgtcccc actagcgtcg cgattcctaa aatcaatact
    18841 aaggatgtga tcacggtata gtcccgttgg ccgatggaat caataaatag ttttccgact
    18901 cccggataac caaaaatctt ttcgataact acagaaccag caatcaacga agtgatttca
    18961 taccccattt gagaagccat cggcaacgct gcatttcgga aaatatgtcg actgaatact
    19021 ttattcgtcg gtaccccttt ggaacgggct gttctgacaa aatcgagcga tttcgaatcg
    19081 attacttcac ttcgcagata ttggattgtg acggcagtcg ccaaaaatgc ttgggtaatt
    19141 gatggtaaca gaatatattt caaacggtct atccattgag tgataaatcc accttgcaaa
    19201 cctagtgttt gtgatccact tgttgggaac cagcctaaac gataaccaaa aacaaatagc
    19261 atgatcaagc cgaagataaa taaaggaaca gcaaaactaa agaagttgta gattacgaca
    19321 atcttatcca agattgaatt ttgataccgg ccagcaagca tccccagagg cacagcaatc
    19381 aaataagtaa ggacaactgt caagatcgat agatataaag tgttgatgat ccgttcgccg
    19441 atcaagtcag caacagagcg tttgtataag aaactttgac caaaatcgcc ttgcaaagca
    19501 tttccgatcc aacggaagta ctggacatac catgggtcat tcaagccagc tgcttgtcgc
    19561 atctgttcga tcactgccgg atcctgattt ggattgatca aaccggtaaa tggatccccc
    19621 ggcatcgctt gggctaataa gaagatcaga acacttaaaa taataacttg cgggatcatc
    19681 aataaaacac gtcgcagaat cgttttccac attagcttgc acctccatct ttcaacgcca
    19741 ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg accagtcgca tcatagtatt
    19801 ctttttggtt ttggatatac tcttgttcta ctcttcgtcg attttctttg tggctttccc
    19861 tattttctac atcgatttgc ggaatagctg aaagcagacg tttcgtatag atatgtcgcg
    19921 ggtcattata aatgtcttct cttgtaccga tttctacaaa tcgacctttg tacataattg
    19981 cgatattgtc gcacatgtgt tttactacac ctaaatcatg tgaaatgaat aaatagctga
    20041 ggccgtattc ttgctggatt cgtttcatga aattcaatac ttgcgcttga acagatagat
    20101 ccagagcaga tacaggttca tcggctacga tcagttttgg attggtagca acagcacggg
    20161 ctactcctag acgttgacgt tgtccgcctg aaaattcatg aggatattta tatagcgcat
    20221 cttccggcat cccaacgata tctagcagtc cttttacctt tttcttttct tcttgatcgc
    20281 ttaaccgttc aaaatttcgg atgggttcag caatgatatc caacactcgc tttttaggat
    20341 tcaaactgga cattgaatct tggaagatca tctgcacatc cttgttataa cccatttttt
    20401 tgcggttgct tcgcttcgtt acatcttttc cttcatacat gatttgtccg gctgttacct
    20461 tttctaatcc cacaacggct tttcccgtag tagattttcc agagccagac tctcccacta
    20521 aaccatatgt ctttcctttt tcgataataa aatccacacc atctacagca tatacatgat
    20581 ccgttactcg attgaagaat ccactgcgaa tagggtaatg aactttcagg tctttgattg
    20641 tgattaattc tgtcatctac acctctcctt tttcgtctct aaagtggaaa tgcttatagc
    20701 aggagcaacg taccaagtgg ttcggtgcga cttcatgaag gataggctct tcttcatgat
    20761 cgcttgctgg tatccatgga atacgtggtg caaaccgaca acctttacga ggcatgtttt
    20821 tcagagaagg aacgacacct tcgatcacat ggagttctgc atcatgattt ccttcttgtg
    20881 gtatagagtt cagtaaagag cgtgtataag gatgcttagg atgatcaaac agttctttta
    20941 cacctgctac ttcaacgaac tgtccgccat acatcactgc cactcgatca gccatttctg
    21001 ctacgactcc taagtcatga gtgatcaaga tgatccctgt ttttgtttct tcctgaaggt
    21061 ctttcagtaa atcaagaatc tgtgcttgga tggttacatc tagggctgtc gtcggttcat
    21121 ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc gatgatgaca cgttgtctca
    21181 taccgcctga tagttcatgg ggatattggc gtccaactct cgcaggatta ggaatcccta
    21241 cttgttctag caattctagt gcacgagctt gacgctgttc tgcattcatc tttgtgtgat
    21301 aggaaagtcc ttctttgatc tggtcttcga ttctcatgag aggatttaat gcagacaacg
    21361 gatcttggaa aatcattccg atatcatttc cgcggatttt attatagagc gtttcgttca
    21421 ggtcgattaa attcagatcg ttatataaaa tctcacctgt aatttttgtg ttattaggat
    21481 caagtaatcc cataatcgta gtagccaagg tacttttccc gcacccagat tccccaacga
    21541 ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc atctactgca tcgtaatagt
    21601 catctttgag tcggaatcct gtatgtaggt tctgaacttc taataattgg ttatcggaca
    21661 atcgaatacc cctctctgct tcctaaatgg aagtttttct taataaaaaa ggacattaca
    21721 cagaat
//
LOCUS       DAZULO010000042        21340 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000042 DAZULO010000000
VERSION     DAZULO010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.043"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..839
                     /locus_tag="ACG8OA_001530"
     CDS             <1..839
                     /locus_tag="ACG8OA_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4976323.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
     gene            complement(1192..2040)
                     /locus_tag="ACG8OA_001531"
                     /pseudo
     CDS             complement(1192..2040)
                     /locus_tag="ACG8OA_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2193..3305
                     /locus_tag="ACG8OA_001532"
     CDS             2193..3305
                     /locus_tag="ACG8OA_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4976324.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3383..3676)
                     /locus_tag="ACG8OA_001533"
     CDS             complement(3383..3676)
                     /locus_tag="ACG8OA_001533"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFS4976325.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3844..4158
                     /locus_tag="ACG8OA_001534"
     CDS             3844..4158
                     /locus_tag="ACG8OA_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFS4976326.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4179..6158
                     /locus_tag="ACG8OA_001535"
     CDS             4179..6158
                     /locus_tag="ACG8OA_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4976327.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6171..6497
                     /locus_tag="ACG8OA_001536"
     CDS             6171..6497
                     /locus_tag="ACG8OA_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFS4976328.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6513..7031
                     /locus_tag="ACG8OA_001537"
     CDS             6513..7031
                     /locus_tag="ACG8OA_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976329.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7031..8383
                     /gene="celB"
                     /locus_tag="ACG8OA_001538"
     CDS             7031..8383
                     /gene="celB"
                     /locus_tag="ACG8OA_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS4976330.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8467..9972)
                     /locus_tag="ACG8OA_001539"
     CDS             complement(8467..9972)
                     /locus_tag="ACG8OA_001539"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFS4976331.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10064..10654)
                     /locus_tag="ACG8OA_001540"
     CDS             complement(10064..10654)
                     /locus_tag="ACG8OA_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4976332.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10833..12479
                     /locus_tag="ACG8OA_001541"
     CDS             10833..12479
                     /locus_tag="ACG8OA_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFS4976333.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12528..13247
                     /locus_tag="ACG8OA_001542"
     CDS             12528..13247
                     /locus_tag="ACG8OA_001542"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS4976334.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13726..14673
                     /locus_tag="ACG8OA_001543"
     CDS             13726..14673
                     /locus_tag="ACG8OA_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4976335.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14685..15605
                     /locus_tag="ACG8OA_001544"
     CDS             14685..15605
                     /locus_tag="ACG8OA_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4976336.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15646..17103
                     /locus_tag="ACG8OA_001545"
     CDS             15646..17103
                     /locus_tag="ACG8OA_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4976337.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17448..18062
                     /locus_tag="ACG8OA_001546"
     CDS             17448..18062
                     /locus_tag="ACG8OA_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS4976338.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18055..19779
                     /locus_tag="ACG8OA_001547"
     CDS             18055..19779
                     /locus_tag="ACG8OA_001547"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4976339.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19776..21227
                     /locus_tag="ACG8OA_001548"
     CDS             19776..21227
                     /locus_tag="ACG8OA_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4976340.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6880 a         3616 c         4324 g         6520 t
ORIGIN      
        1 catatcagct ggtagaaact caagcgccaa cggggtatga attagatgcg acaccgatcg
       61 agtttgagat cgaaagaagt cagacagcgg tggtagaatt gacgaaagaa aaccgactta
      121 caccaggcgg tgtcgtgttg acaaagatcg atgaccaaag cggggaagtc ctgcaaggag
      181 ctgtttttga gttacaagat gccaatggaa ccgttctgca gtcaggtcta accacaggag
      241 cagatggaaa actggcgatt gacggcttag caccaggagt gtatcagctg gtagaaaccc
      301 aagcgccaac ggggtatgaa ttggatgcga caccaatcga gtttgagatc gaaagaagtc
      361 agacagcggt ggtagaattg acgaaagaaa atcgacttac accaggcggt gtcgtgttga
      421 caaagatcga tgaccaaagc ggagaagtcc tgcaaggagc tgtttttgag ttacaagatg
      481 ccaatggaac cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgatag
      541 atggcttagc accaggagca tatcagctgg tagaaactca agcgccaacg gggtatgaat
      601 tggatgcgac accagtaact ttcaagatcg aaaaagaaca agaagtggcg atctttttaa
      661 caaaagagaa tagaaaagtt gctgatagct cagatataag aggaaacaat ccaacaagca
      721 gtggaaataa gcatcttcca aaaactggag aaaccatcat tagccagttt atattgtcaa
      781 tattgggtgt gctacttgtt ttcatcagta taaaatttag aaagaaacga aatatatgag
      841 ctaaaataaa aaattgaata agcatagttt ttaaaatcaa ctagtaccca tcaattgaat
      901 ggaaactacc gattttagaa ggggtataca aacaaatatt agtatcggag tagagaaggt
      961 tgaacccaac cagtaaataa gtaaagacaa ttttgtgctg cgttgagaag tgaaaaataa
     1021 acctttgtag taatactagt taataaaaaa tatacaaaaa cactgcgaaa atcattcttg
     1081 agtttcgcag tgttttttgc ctaatgactc aagtttattg ggtaatactt tgattttctt
     1141 tctacaaata atcagcgatc agttgtaatt ttttctctag ttttaatggc tctttgtcaa
     1201 taaggagaaa aacaagtcgg gtcttttgtc tcggaaagct tcaaccaact cgtgaataaa
     1261 ggttacgcct ccttgaaagg cgtggaaaaa ccaagtaatc gatcaatcac caatttcgca
     1321 tttggtagaa catgtttggt gagctgaaag taggtggcgt tcatgtctgt aactaagaat
     1381 tctatttctt taggattagt gcagctaagg aaatagtttg ttaatcgagg aattttttgt
     1441 gtaggtaaat tttctcgatt aatttttctg tctcgccatc cgcggaaata aaactcagtt
     1501 tatcctcaac ggaaacgtga gaacgaaatt cacaaccctc aacactcaag acagaattct
     1561 tttggaagac tttggtaaat agcttctcaa ctctttcaat gtgcgaataa cggttgtcaa
     1621 agatacctaa caacttttcg aaataaaaaa taacgacact ttttcagtca gtaaagaagt
     1681 gattttatgt ctgacatgat ttgaaatcga gtgccttgga cggataaaat aactttgagc
     1741 gttccaatgg cttcgacagt ttttacaggt atagcgctgc ttttttaggc gtataaccat
     1801 tggcatatga ttatattatt caaagtggac aatggtttcc ttcttcttgt ttttcaccac
     1861 cattaatttc ctatttccat ctaccacagc agaaccacaa tttctacaga cacatggagc
     1921 agggaagaga actgcagcaa cgatcaatgt ttttttcttc tgtaaggttt cgtaagagac
     1981 ctctgtaatc atcaaatttt tgtctattga tctcagaatt tttttgatag aaccattcat
     2041 aaagcatatc gttctctcag ttgtttattt tgtggtgatt taatcataca agaaaacgat
     2101 atgttttttt ataaaaacat gaataggaaa aatagttgac aagaaaatga caacgccata
     2161 tgatgttcca tagtatttac aacctgtcat agatgactgt aaaaaataaa agtaaaaaga
     2221 aaggacgaca acaggtggat ttttatcagt ctctacaact agatccgttt attttaaaac
     2281 aaaaaatacg agaagcagct tctaaaaaag aaaagtgcat gtatatttgt tcattgtttt
     2341 tacgatcatt attcatcgta ttgtttgcca tttgttttat cattataatt acaacattat
     2401 ttgaatcgaa gcataaacca tacgctgtgg ttttattctg tatgttgatg agtattcgtt
     2461 ttgttgactt tggttataag atttcccatt cgatcatagg tttagcaatc gtcatgttct
     2521 cactattagt agcaccgtat gtccagctga tcaaatggtc agttatgggc atgttgattc
     2581 attttatttt actaagcagc atattaatgg caaccgctag tgatcccaaa atggggaatg
     2641 cttctttgta cggcttttca tatctcttta ttgtttattc gttacctaaa gattcgttga
     2701 acaagaattt ctttacacaa acaagtagct tattattttt gtttttttgt tggttttcag
     2761 ttctccttta cagaaaacat cgagaaaaga acaaagacaa aagcttgttt aaagagattt
     2821 ttttgaaagg catgtattcg caggaaaaaa tttggatgct catctatgca tttggcatta
     2881 gtcttttgat cgttgcagga gagtatgtcc cttttcagcg attgatgtgg gcagggtttg
     2941 cattttcttc tattgtatct agctatggat tgatgtcgat tggtttcaaa gaacgagcag
     3001 tagaccgaat cattggctca ttgattggat gtgttttatt tattggtatc agccaattta
     3061 ttccttttaa ctgggtcggt atattaggtg gtctagcttt aggtgtttgt tctacctata
     3121 gatataaaac agtttttaat tcttttggtg ctttggcaat cactgccagt ttattcgggg
     3181 ttccaggagc agtaacgatc cgcatttttg agaatatcct tggggtatgt ttaggaatca
     3241 tgtatattgg ggtgactgaa atacttatcc gaaaaataag agaaaaacac ggtttgaatc
     3301 attagatgag atcgaataaa aaataaggaa aaagtgaaga tacacatgga tgataaattg
     3361 ctatcattcc atgtgtatct ttttacggta aatcagtgaa acgttcgact ttcattttta
     3421 atttatactt tttacggagt tcgtttattt gagccatcat ttcggatttg tgatgctttt
     3481 cgattgcttc ttcatctttc caacggtcga tcagtaaaag agattctgga tcttctagtg
     3541 gaaagtaata gtcatatttc tcgtttcctt cttctgcacg aatcgctttg acaatgtttc
     3601 tttcgaccat ttcttctgca aattttctgg cttgtccatt tgtacttgta tacaaaatat
     3661 tcactgtgat actcactaaa tcccctcctg aaaaaattct atcactagta tatcacggtg
     3721 ccagaaatta ttattcaaga tatgatacac attttttccg caatgctacg gaaatatata
     3781 ttttgaatag aaaacgctta caagatatgc ttggtttata aaaataaaag gagtgattga
     3841 tttatgaaaa aagcattgat tatttgtgcg gcaggaatgt catcctcgat gatggcctca
     3901 aagacaacgg atttctttaa gcaaaaaaga gaagaggtac tcgtagatgc agtttcagct
     3961 acagaaggcg ataatatgat taagaatagt gattttgatt tatttctgat cagcccgcaa
     4021 acgacgatgt ttttggataa attcaaaaaa ttaggagaat ctgttggaaa gcctgttgtt
     4081 agtatccctt tccaagcgta tgtcccaatc cctacaggca tccagaagtt agcagaggtg
     4141 atcgaagaga acatatgatg gaataggagt ggatgataat gaaacagaag gaaaaattgc
     4201 tgttgagata tttgattgat cacaagaagg agtatgtcac cagtcaacgt ttagcttctg
     4261 aattgttatt atctgatcgg acaatccgta attacttgca gcggatcaaa gagttagtag
     4321 aaaaaaacgg aggtaagatc attgctaaac caggttatgg ctaccaactg catattttac
     4381 aaagactgac atttgacttg tttttgagtc ggcaagaaat agtacctgga tatgctagag
     4441 aaccgcaagg attttatgag tccgaagatc gacaaaagta tatattgaac caattgcttt
     4501 tggaagatgc cattcttttt atggatgatt tggcagaaga gctttatatc agtcgttcca
     4561 gtttgacaaa ggacatgcaa gaaatcaagg agcgattgat tccatattca ctaaaaatcg
     4621 tttctaagca tggtcaaggg acatggatcg aaggggaaga aagaaatcga cggcatttta
     4681 tcatggatac tttttttgga aaccattatg gaaattcatt gaaagaatat ttagggcaaa
     4741 gccgtttttt cccagacatc agttttgaag agctagtcat tatcattctc gatgaaacaa
     4801 gagaagctaa gttaaaagtt tcggatttta tcatacagaa tctgacttta cacttggctt
     4861 tggggatcaa acggctccga gaagggtttg aaatcaagga attaggcatc gaacaagaag
     4921 tttctaagcg agtagagtat caagtggcgc agcggatcgt tcaacgtatt gaggtaatcg
     4981 caggtgtatc gtttcccgaa gaagaaattg cttatttgac attacatctc atggctaagt
     5041 cgaatcacaa agaaaatcaa aatgatcagg aactatcaaa ggaattagcg aaagttctga
     5101 aaagattgtc tcaggaattt catttttcat tagtagagga tctccagctt ttcaatggac
     5161 tactcgatca tttgtcaccg atgatgattc gattgagccg aggtattcgc attgagaacc
     5221 cattgactga ggagataaag aaagaaaatc ccaaagtctt tgacgcagta aaaaggcatt
     5281 tcagcaacat gccggctttg aaaaactata ccatcaatga agatgaatgg gcctatttgg
     5341 ctttgcatct catggcggca ttggaaaaag aaagagctgc gcataaacta catgcattga
     5401 ttatctgtgc aacgggttac ggcagtgccc aactattaaa gaatcgcgta gtcagtgaat
     5461 tcggtaaaaa tattacagtg gtaagtgtaa aaggctatta tgagatcaat gaaacaactt
     5521 taaaaggaat cgacctgatt atttcttcta ttgatctttc gacaatgttt ttcaaaattc
     5581 cagttttaca tgtcagtatt tttttgaatg aagatgatgt tcgtaaaata cgaaaggtag
     5641 ttggagaatc tataccctat cattcctcag atagaccgat accagcattt tctctacagc
     5701 aaaaaaagca gatttacacc gaacagttgt ctgaaaaatt tttcaagact tatacgtacg
     5761 aaccgaaaaa ggaagaaatt cttcatgatt tgctgcaaaa gctgagcata aatgaagaag
     5821 aaggatatat aagagaaatg cgaaagcaga tccagcaacg tgaaaatatg ggacaaatca
     5881 tattcagtga tacagtggca gtcccacatc ccgctgttcc agttggaatc gaaacaaagg
     5941 tagccgttgc attgattcct tctgggatgc agtgggatca atatcaagaa attcattttg
     6001 tttttttagt ttcgccttct tatgtcgaaa atgaaggaat tacggtcgtg actaaagcaa
     6061 tcgttaggtt agttgaccag ctaaaagtac agcaggaaat cttagctgaa cctatttttg
     6121 agaatttcag cagtaaattt atcaaactga tcgaataagg aggaaaacag atggctgaaa
     6181 aaataaacag tgaaacacta caagtgactt cttttgatat cattttccat agcggaaatg
     6241 cacggacact gatacatgaa gcatttcgct cgatgcgtaa agagatgttt gaagaagtag
     6301 aagagaagtt gcgacaagca aatgaagaga ttcttcatgc gcatcagtca caaactcaac
     6361 ttctgaaaga atatgccagc gggacaaaaa tagaaatgga aattatcatg gtccatgcac
     6421 aagatcatct gatgacgacg atgacattat tggaagttgc acaggaaatg aattatttgt
     6481 atcaaaaaaa taaatagaaa ggaaggatca ttgtgcagga tacagtaaaa aggaaaatcg
     6541 aattagaaaa aaagcaactt agtttgaaaa acatgtattt caatcgttat ctaatcgtac
     6601 gttacctgac tgcctttttc tttttcatga acatgcaatg gatgattctg cttctatcag
     6661 ctaaaagttt aggaagtagt ttaccgatga ttctgatttt agctattttg cctgcagtag
     6721 gagaacaagt aaagctttat cgaaaacacc aaacgaacgt tccatggaca aagtggtatt
     6781 ttttgttcca gagtgtcttt aatttattag tgattccagt gttgtttacc tcaggattta
     6841 cattcttgta tccttttatg gcaaataaca atggaggaca gttatttgtt ttcattcttg
     6901 ttgtaagcgg tatttttgcc agtgcattta tacagtatcg tttaaagaaa atttcactca
     6961 accaagacca acagtatatc cgtattaaac aatatgaaaa agcgttatat ttaggaaagg
     7021 agaataacta atgtttgatt ttttgcagaa atacctgatg ggacctatgg ggaaaattgc
     7081 gcaacttaaa atcgttcggg ctgtcatggc agcggggatg gcttctattc ctttcacgat
     7141 tgtaggctcg atgtttttgg ttttaaatat tttgccatta ccgttcccat ttttagaagg
     7201 attttttaat gcaacttttt tcaaagttag tgacttgtac atgatcgtca atacaatgac
     7261 gatggggatt ttatctattt actttgccat tgtgtttgct tatgaattaa cgtcaattga
     7321 acgagatgaa caaaaattaa atgttaatcc gctcacgggg gcattactat ctgtttttgc
     7381 attctttatg tgtatccctg aattgatcat gactgatggt aaaatcagct tgattgcttc
     7441 tatcaatgag acagaaactg tagtaaatgg tattaggatg ggctcttttg tcgaacgttt
     7501 aggaacttca ggaatcttta cagctattat tatgtcgatt attgcggtcg aattgtattg
     7561 tatgtgtgtc aagagaaatt gggtcataaa aatgccagat gttgttccgc caggagtttc
     7621 tcgttctttc actgctctga tcccaacatt tgtcattgcg tttgtggtga tgatcatcaa
     7681 tggattatta gttgcactag gaacggatat cttcaaaatg attgctatcc catttgggtt
     7741 tgttacgcag ttgacgaata catggcttgg tgtcatggtg atttatttct tgatccatgc
     7801 attgtggatc gtagggatcc atggagcgaa tatcatcact tctttcttga ccccgatcgt
     7861 attagctaat atggcagcta acgcacaagg cgcaaattat ccgttggcag gagaattcaa
     7921 caattcttat gtaaccgttg gtggatcagg tgcgacttta ggattgatca tctttattgc
     7981 attcctcgcg aaatctgatc agctgaaagt gttaggaaaa gcctcacttg taccaggatt
     8041 attcaatatc aatgaaccaa tcattttcgg gatgccaatc gtctacaatc cctatttagc
     8101 ggttccgttt ttcttagctc cgatggcttc tgctacactc gcttattttg ccattcggct
     8161 ggaaatcgtc aaaccgatgc ttgcacagat gccgtggcct tcaccagtcg ggatcggagc
     8221 atttgttggt agtggtggtg attggaaagc tgctgtgtta gcagtcattt gtgcaatcat
     8281 cgcattcatt atctggttcc catttatcaa attctatgat aataaattat taaatgaaga
     8341 aagagaaaag gcagcagaat tagctagtga aaagttaaaa tgatttctaa gaaaaaagga
     8401 actgtgacaa aaaatcacag tccctttttt tcttagaaat gggaaaccac tagtatataa
     8461 ataccattat tcttcaatca accgcttcac gattgcataa tccccttcat aaggttcatc
     8521 tacaccattg attttttgat agggatccac gccttttccc gcaataacga ctgaatcttg
     8581 cggagcgctt tctgctaaag cgcgtcgaat cgcttcagga cgatccattt cataatgaac
     8641 gatcaaatct ggattagtaa ttgcttcatt gatttcttcc gcgatttttt tcggatcttc
     8701 aaatgctggg tcatctgccg tcaaaaaggc gatgtcagct gtttctgata gcacttttcc
     8761 aaaatcatgt cgacgggaga ttgctttgtt tcctgggctg cctaatacaa caattacacg
     8821 gccatctgga tgttcatttt tggcaaattt cagcaatgtt ttcaagctta gataattatg
     8881 tgcatagtcc acatagacat gcgctccatt ggattggatc aattgatcca ttcggcctgg
     8941 tacacgagct tcttttaatc cgtcttgtgc atcttgactg gaggcacctg ttagtgctgc
     9001 ggcaatgatc gcactggctg cattttcttg gttgaatcct ccagccagca aaatatcata
     9061 agcagtatcg cagatagcta agcgatcttc atgagaagat aaagtaaaac cgtgagtacc
     9121 tttatctgaa cggatcactt gataattgct ttcttccgaa cgaccataag tgatcgttgg
     9181 aatctggaat agatcggctg tttccctaag taaatcaaaa tagtcacttt catgatttaa
     9241 aacaactgtt tttgaattca agatcaattg tcgcttgcaa tagaaatagt catcaaatgt
     9301 cggatgttcg atcggactga tatggtcagg cgagatattc aagaagattc cgacatctaa
     9361 agtcaagccg tatacgcgtt gtgttttgta agcttgtgaa gaaacttcca tcactagatg
     9421 agtcatacca ttttcaactg cttctgacat catacgatat aaatctaatg actcaggcgt
     9481 tgtcaaatgg gatttgaaat atgttttgcc atctaacgtt gtattcatag tagaaagcaa
     9541 tgctgttttt ttatttgttg tgtgatccaa gattgctttt gtaaagtacg cagcagttgt
     9601 tttacctttt gttccagtga atccgatcac tttcaattta ttttgcggat agtcgtaaaa
     9661 agccatagca agaagcgcca ttgctttacg gatatccgtt acgatgattc ctactgctga
     9721 attttcgtat ggttgttctg atacataata ttgaataccc tgttcgattg ctgaatccaa
     9781 atattcttct ttgaaattca ttcctttaca gaaaaacaaa gtagaagcat cagcttttct
     9841 tgaatcataa cttaactgaa gaaattcagc tttttctggt acatcaagat gccagcctct
     9901 tgttgaaaca aattctttta atagattttc ttttaatagt aaatgacgga tgtcttctaa
     9961 agagaagctc attgattgat ccctcgcttt attcaatttc ttggtttatt atagcacact
    10021 ctttttaata tatcgtttgt ttccgctatg acaaaatcca gctttattca aattttcttg
    10081 acacagggga caagcggatg agtattttca taaacaatga actgactgta atagatagga
    10141 gaacgaatag tgtctccaac aatccaaaaa cataataatt caaaaagacg ataagactat
    10201 tggtaagtaa aagtccttgc ggcattggaa gccgaaaaaa tttgtggaaa ataatcggcg
    10261 gtatcgttat accgccattt gaggccccga ctgtgtacaa tgtgtaaacc ccaagactga
    10321 acaaaagact tcctgaaatc agtgaaaaga aatgtgagga gatcatttcc acttttggaa
    10381 caatctctaa aacaacagga aaaagcagac ttcccgctac agtcttaaaa aatactgatt
    10441 ttcctaatag gacagcggct agaagcagca tcataagatt gatgctccaa acagtaactg
    10501 agcgttggat agaaaaggcc gtttcaacta aatgtccgat ggcgcccact ccggcggaag
    10561 ctatatcgtg agggagcaag aaccaattca atccaactgt gataaagaaa agacctaaaa
    10621 atataacggg tagttttttt atttcttgtg tcattgatat ctcctataaa tgttagccat
    10681 aaaaggctgt atatttcttt actttaccaa aaaaacgaaa ccgaattcaa aaaaacaatt
    10741 gttcttataa aagttatata aaatcgattg ccagtttctg ttttaccaat taaaatgata
    10801 gtaatgtttt gtagaaagtt ggaatctgac cgatggataa tcaaagaaaa ccagcattag
    10861 atcaattaac accgcaagaa aaaatggcac gaggctctgc ttggatgacc gccagtaata
    10921 tcatttcaag attgctaggc gctgtctata tcattccctg gtatgcttgg atgggcgaaa
    10981 atgctaaggc tgccaatgga ttattcaata tgggatacaa tatatatgca ttgtttctac
    11041 tgatctctac agcaggaata cctgctgcga ttgcaaaaca aactgcacgc tataattcat
    11101 tgaatgaata tggcacttca cgacgattat ttatccgtgc attacagatg atgggcgggt
    11161 taggcctgtt gtttgctttg ttcatgtaca tcgcctctcc ttggctagct catgcttctg
    11221 gtggcggaga agaactgatt ccaacgatgc gttctttgag tatcgctgta ttggtttttc
    11281 cttgtatgag tgtgatacga gggtatttgc aaggcaatca agaaatgatg ccttatgcgc
    11341 tgtcgcagat tgtagaacag gtggcacgta ttttttatat gctattggct acattcatca
    11401 tcatgaaagt catggatggt gactatgtga cggcggtcac gcaatcaact ttcgcggcat
    11461 ttatcggtat gctagcaagt attcttgtcc ttttgtattt tttgaagaaa catcaagcgt
    11521 atacatcggc gtttattcac tacagcgaaa acaaaataaa tattgcaacg aaggaattgt
    11581 tgttagatac cgttaaagaa gcgattccgt ttattattgt gggttcaggg gtaacgatct
    11641 tcaaattagt cgatcagttc acttttatga agatcatgtc tgcatccacc gagtattcca
    11701 atgcacagtt actggatctt ttttcgattt ttagtgccaa tccagataag ttgacgatgg
    11761 tagttattgc attggcaaca tcgatcgcgt ctactgggtt accgttgatt acagaagctg
    11821 ttacgttgaa agatcgagta ggtctggcaa aattgacaag cggaaattta cagttgtttt
    11881 cattttttat gtttccggcg acatttggtg ttatgttact tgcctatccg ctaaatacat
    11941 tattttatac accggattct ctaggaagta atgtactgat ccaagcaagt tttgtaggac
    12001 tcttcctagg actatatatg ctagtttcca acatgctgca aggaatgttt gaaaacaagg
    12061 cggcaattaa ttatttggtt attgggtttc tagtgaaact ccttcttcaa tacccagcca
    12121 ttcgtttatt tgaagtatac ggccctttat tggcgacaat gatcggattt gctgtgtctt
    12181 gtaccttgat cttaaagaga atccatgaag tagcccgctt taaaccaagt tttgtctggc
    12241 gcaggacatt gctgatcttt attcttacct tgattatgct tttggcttcg tggatcacaa
    12301 agatgatttt tggtgctttt ttaaacgaag atagcaaatt ccagtctcta ttgttgattc
    12361 ttgtagtagc aggtataggg gctttagtgt atggttattt agctttgaaa atccgcttgg
    12421 cagaaagact actaggacca ggaatagcgc gtttacgaca taaattaaaa ataaaataag
    12481 tgaatcagaa gagctagatt gatgctcttt tgaaatgggg gaaacgcatg cgtttagata
    12541 agtttttagc agatatggga cttggcagcc gcaaagaagt caaaggatta atcaaaaaag
    12601 gaacgatcga agtcaatgga caagtggtga aaagcgataa agcgcaagta aatgaattcg
    12661 aagatgcggt tacttattta ggagaaatca ttacgtatca aaaagatttt tactacattt
    12721 tgcataaacc tgcaggagtc atttctgcta ctcaggataa tcatgatcaa accgtaatcg
    12781 atttattgga agaccaggat tatcgggaag atctttttcc agtcggccga ttagataaag
    12841 acacagaggg cctcttgatt ttaacgaatg acggggtgtt tgcccatcag ttactatcac
    12901 ctaaaaaaca tgtggagaaa gaatatctag ctgaggttag cggtataatg acagaagaag
    12961 atatccgttt atttgcagaa ggactgatta ttgatggcga tgagcagaca ttgccggcaa
    13021 aactccaaat tgatcaagta gatcaagaga cagaaacttc aattatccgt ctgattttgc
    13081 atgaaggaaa gtttcaccaa gttaagcgaa tggtcaaagc agtaggaaaa gaagtcttat
    13141 atttaaaaag aatccgtatg ggaaaatttt ggctgcctaa agatttgaat aaagggcaat
    13201 atcgacccat gaccaaagaa gagctagaac aagtgagaga aaaataattg aatggatagc
    13261 taaaagaata agcagagtag ttgtagcgcc atctgaaaag cagacttgtc cgtctgactt
    13321 tcggatggct tttttgtgaa atttcggctt atttccgtag aagctacttc tggaacaccg
    13381 tttgttcgga ataagggact gtgacaagac ttttgttaca gtccccattg ttccttgtga
    13441 ctcagagtta gatagtaagg acacgatctc cctaatactc aggccaaaac gctgaattca
    13501 caacctctga taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa
    13561 aatatgagga actattttcc cgtttttttc tctaatactc aggccaaaac gctgatttca
    13621 caacctctcc tatactttgt ttcattttcc ctatactttg tttatttatt ctcattattg
    13681 aaagcgctat aattacgttg cgaatgaatc gaaaggggtt tttagatgaa aaaacgaaag
    13741 gggttcaatc atttttttat gaatctatgg cgttataaag cacttgtcct gatggctgta
    13801 ccgggtatgc tttggatgat ttttttcttt tacatacctg taatggcgaa tgtcgtagca
    13861 ttcaaaaatt ttcatatttc agcagatggg ttcttggcta gcttgaggga aagtccttgg
    13921 gtgggttggg aaaatttcaa atttttattt tcttcggatc aagcatttct catcactaaa
    13981 aatacaattt tatataatgt agcatttatc cttttgaatc tgctgatctc agtcgtattt
    14041 gcaatcatta tgagcgagtt gagaaataaa cgactcgtta aagtttatca aacgatgtcc
    14101 ttactacctt attttctatc ttgggtcatt atcagttact ttgtttacgc ttttttaagt
    14161 cctgacaaag gaatattcaa tcagtggatc actggacatg gaggagaagc tgtcaattgg
    14221 tataatgaac cgaaatattg gccgtttatc ttggtgttca ttggtacatg gaaagggatt
    14281 ggttataaca gcattattta ctttgcatct gttatgggaa tcgatccgac ttattatgaa
    14341 gctgctatgg ttgatggagc tagcaaatgg cagcagatca aacatgtgac gataccacaa
    14401 cttattccac taatgacgat tttaacgatt ttagccgtag gaaacatttt ccgagctgat
    14461 tttggcctgt tttataacat tcctaggaat tccggtgcat tatatgatgt gactcaagtt
    14521 ttggatacgt atatctacaa tggcctgaca tccacaggag attttggtat gacggctgct
    14581 gcaggacttt accaatcagt tgtcggcttt gtcttgttga tgatcacaaa tacgatcgcc
    14641 cgacgttttg atagcgattc tgcattattc taaggaggaa aaaaatgaaa aaaaagaaag
    14701 tggagagagt ccaagtccgc tctttcaata agacggctaa ttttttctct aatataatga
    14761 ttgcactctt tgctatttcc tgtattttgc catttatatt tgttatcgcc atctctttta
    14821 ccgatgagtc catattgtcg actcacgggt acagtttttg gccagaaaag ttcagtatat
    14881 ttggttatac gtacttgttc gaccagatgc aggataaaat tttccaagcg cttttcgtca
    14941 ctgtacttgt tactgtttta ggtacactga tcaatagcac ggctacttct ttatatgctt
    15001 atgcgatttc acggtcaaac tttccattcc gtcgtttttt taccgttttt tgtttgatta
    15061 cgatgttgtt ctcaccaggg atggtggcca attatcttgt tatgacaaat cttcttcagt
    15121 taaaagatac gatttgggca ttgatcttgc caatggctgt cagtcccttc aatattattg
    15181 tgatgcgcac attctttaaa cgttcggtct ctgattcgat tattgaatcg gcacggatcg
    15241 atggagctag cgaactgcga atatttgttc agatcgtttt accgttagct gttccaggta
    15301 ttgcgacaat cagtttattt gccgcattgg gttactggaa tgactggttc aatgctttac
    15361 tatacatcca agatgataag ctagttccac tacagtatct tttgatgaaa atccaaagta
    15421 atattcaata tctgacacaa aattcaggag caggaagcca attgacaggc ggattagcct
    15481 cagttccagg agaatctgcc agaatggcga tcgtagtcat ctcgacgttg cctattgcga
    15541 tcagctatcc atttttccag aagcattttg tcaaaggtct aacaatcggc ggagtaaaag
    15601 aataaagaaa caaaaatata aatcataaaa ttaggaggaa gaaaaatgaa gttatggaag
    15661 aaactagcct ttacaggtgt ttcagcgttg ttgttgggaa cattagcagc atgcggaggt
    15721 tctggttcta aagaccaggc agaagctagc aattcggaca ctttgcagat gtatcaaatc
    15781 ggagataaac cagataattt cgatcaattg atggaagtag caaacaaacg aatcgaaaaa
    15841 gagatcggtg taaaagtcaa tatcaattac attggctggg gcgattacga aaagaaaatg
    15901 aacgtcatca tttcttccgg ggaaaattat gatatcgcct ttgcgaacaa ctatgtttcc
    15961 aatgcccaaa aaggagcctt tgctgatttg actgaattag cgcctaagta cgctgaaaaa
    16021 gcttataatg atttagatga agcatatatt aaaggaaatc tggtcaatgg aaaattatat
    16081 gctttcccag tcaatggaaa tgtctttgct caacaagtac ttacttttaa caaagcttta
    16141 ctagataaat acgatctttc aattgatgga atccaatctt acgctgatgc agaaaaagtc
    16201 ttgaaggaat tccatgaaaa agaaccaaac acggcagctt ttgccattgg acaaggcttc
    16261 aaagtccaag ggaactttga ttatccttta gggaatacgt taccatttgc tattgatctg
    16321 gatggagatg attcgaaaat catcaaccaa tacgataatg aaacgtatat cggactgtta
    16381 cgtacaatgc atagctggta caaacaagga ctcattccaa gtgacgctgc aacgagcaat
    16441 aaggattatc cactagaagg aaatacttgg ctaatgcgtg gagaaacaca aggaccgtat
    16501 gattatggcg atacaatttt gacaaatgca gcaaaacaag aacttgtctc aaaagcaatt
    16561 actgttccac taaaatctac agctcaagca caaatggcaa attttgtcgt atcaaatact
    16621 tcaaaaaata aagaaaaatc agtagaactt ctagggctgc ttaacagtga tccagaatta
    16681 ttgaatgggc ttgtttgggg gatcgaggga gaagcatggg aaaaagtcga tggttcagat
    16741 gataaaatca aattgctaga tggttatcag ccaaatacac atatgtctgc ttggaataca
    16801 ggaaacaaca agattctata tacacaagag tctattacgg atgacatgat tgccaaacgt
    16861 gatcaatcga ttgctgatgc agaaacttca ccgattttag gattcagctt caacactgac
    16921 agtgtcaaaa cagaattaag taatatttct aatgtcatga atcaatattt agatggattg
    16981 aatacaggga ctgttgatcc agatgaaaca ttaccgaaat tgaaagacgc tttggacaag
    17041 gctggctatg ataaggtatt gaaagaaatg caaaaacaat atgatgagtt ccgacaagaa
    17101 taataatgaa taaagacaaa ggggttgtga caaaagacgt gtcacaacct cttgttccga
    17161 ataaacggtg ttcaaaagcc agtttctcgg tattaagcta aatatatgta aaaatgtgga
    17221 aagccatatt cacaaaattt ttcttactac tcgaaactaa gcggctattt cacaaactct
    17281 tggtaaacgg tgttcaatca gctaaccctc ggtattcgct cacaaaacga caaaatatga
    17341 ggaactattt ttccgtttgt ttctctaata atcaggtcaa ggtgctgatt gcccaacctt
    17401 tttttgtata atgagaaagt ataaacttaa tgtttggagg agaagatatg ttagggaagg
    17461 ttttggaaga gttatttatc cggatctggg tcattataaa actaaccttg tatttctgga
    17521 tatatacatt tgccggcggg atcatctttg gattaggtgc ggcatggaaa acagtcaatg
    17581 aactatttta tttgtacgga ttcgagtata aagaaatcac aataaaaaga ggctggaata
    17641 tatataaaag aaatttttta agaggcaatc tgctatttag tttattttta tctggtactg
    17701 ctttattgag ttacaattta tatttatctg ttcaaatcaa agggctcatt tttttagcta
    17761 ttgattttat tttacttttt gccttgctct gcctatttgc tacttatcaa tacagtatga
    17821 ttttagatag tgaatatacg atctctgttc ctaatctgct aaagctaagt tttatttcgg
    17881 ttttttccag cttttcgtct tttctaaaaa taattattgg tagcggtata attttaggcg
    17941 tgacttggca attcaaagga ttgatcctgt ttggtgtcat tggtcttttg acggtttgga
    18001 atggtacgat gacaacacat tggcgtgaag aattagataa acagttggaa agttatgaat
    18061 aagtttcgga ttctattttc aaaaggaacc ttactaaatc gtctgttgcg gaaatacgcg
    18121 gttatcatga tcggtgtaac gatgactgct accgttattt ttagtatcca tacttgggaa
    18181 caaaatcaaa aacaggcaga aaacatgaca agtgatgccg ttcaaagcac aagcagaatg
    18241 ctgaatgata aaacaacctt gagtaggatc atcaaaaacc agcttgtagg gaattccgaa
    18301 aagatcgaaa atgtgacgac ctacttaaca aaacccatag atcagtatct gatgtatgta
    18361 tacgaacagc aaaacagtac cgatgaatta gtttctttcc caaatcaaat caaagatctc
    18421 tacgcaaatt atgaagaact aagtgcgata tatatcgtgt tgaatcaact accggagtat
    18481 tatgaatctt ctagagaaaa caaaggtggg gaaataaaaa aggggacacc aaaattacta
    18541 gacgcctttt atatcaagtt gcctatcaca caaggaggag ctgagtcagg aagtattttt
    18601 attggttttc aaaagaaaga actagatatg attttagaaa atctaacgtc attcaatgga
    18661 ctctctttat acatgatttc tggaacgaca aatcgactat acacattcca tgataataaa
    18721 atagctaaag aaacgtttga aagacaagaa atgatcatca cagagagtct gaagaaaaat
    18781 tcacaattgc cgattgacga tctggaaaaa aacaattttg ttcaacacca agaactgtct
    18841 gatgattatc gtatattagc tgtactagat aaaaaaagtg taaaaatcga aacgggaaaa
    18901 aatcttgccc cgctgatcat tggtatcatt attttagaca atatactgct tatctttctt
    18961 tataaaacat tcaaacgata ttcacagcaa gtatctatcg tcatgcaggc catggatcaa
    19021 acagcagcag ggaatttaga gacgcggatc gacataacaa acacggaata tgaactgagg
    19081 gaactttcaa tcggtatcaa cgaaatgttg gatagcatca atcagtttgt agaagacatc
    19141 tacaaattag aaatcaaaca gcaagacgca catatgcggg cgcttcaagc acagatcaat
    19201 ccacactttt tatataatac attagaatat atccgaatgt atgcaatcag cgaaggaagt
    19261 gaagaattag cagatgttgt gtatgcgttt tccgctttgc ttcgaaacaa tacgaatcaa
    19321 gagaaaacga tcactttaaa agaagaactg gacttttgcg aaaaatatgt ctatttgtat
    19381 caaatgcgtt atcctaatcg agtagcctat cattttatga tcgatcctga tttggaaaag
    19441 atcgaagtac ctaaatttgt gatccagcca ttagttgaaa attattttaa acacggtatt
    19501 gattttactc gattcgataa tgcgctaagt gtcaaagtct tgcaggaagg caaacgggta
    19561 aggatcatca tcaaagataa tggaaaaggg atgactgaaa aacggctaaa acagattgaa
    19621 gaaaaactgt ctcatcctaa agtggaactt cacggttcta tcggtctgca aaacgtgaat
    19681 gaacgtttac gtgccagctt cggcagcagt tattatatgt cgcttgagaa taatgagaca
    19741 ggtggactga ccgtttctat tacattcaag gagggatgag cgtgtatcaa gtgttattgg
    19801 tagatgatga atacatgatt ttgaatgggt taaaaaagat aattgattgg caaagtttag
    19861 gctttcaaat agtagcaaca gcagagaatg ccaaagaagg actagctgta cttgaacagc
    19921 gacagattga tttagtagta acggatgtga caatgccaga aataaatgga ctggaattta
    19981 ttgaagcggc tcaaaaagaa cgtcataatt ttgaatttat gattttatca ggataccaag
    20041 aattcgatta tttgaaagga ggtatgcaat taggcgccgt gaattatttg atgaaacccg
    20101 tgaataagtt cgaattggtt gaaagcctca agaaaatcaa aacacggtta gatcagcaaa
    20161 atgaacaaaa aaatcaacaa gaaatctatc aagaactgtt attcagtcaa tggttgaatg
    20221 aagaattgga tgaaataagt gaagaagaaa taataggaca aataagtgag aagcagcata
    20281 ggatagtatt gattcaactg gcaagaactc atgatactct tatcaataga tggttgcaag
    20341 aacggcaaca aatcttttac tatcaacgaa attacggaga ccgtgtattg atcacgttgt
    20401 tgttggaggc agatgaagtt gatggcttct gttgttttgt tcaagaaaac tttcctgatc
    20461 aggaatggct gatcagtatc ggggaagaga cagattcttt ggacaaaatc cctgaaacat
    20521 tccaacaagc gaaagacaat ctacagttgc atcaatttta tggagataaa gacgagcatg
    20581 ttttttacgc agtacaagca gaaacaaaag atcaaaccat cgatttttca agtttcagac
    20641 gcgtgctgca aaataaacaa ttggaagtcg ctcaaaaaat gatcactgat ttttttgaac
    20701 agtttcagtt agcagccatg atgccggaag atatacgata ttccagtttt ttactgttta
    20761 tggagattca acgggaattg attgatctgg aagatgagga atatttacaa ggaatcgaaa
    20821 aaatccatca ggcaaaaacg gtgcaagaac ttcagcagct acttctgtca ttcatcaaaa
    20881 agcatcagag acagaaaaag tacagtgaca atgtggaaaa agtaatcgta atcctgcacc
    20941 agcattatca agaaccactt acactcaaag aagtgtcaga aaacctgcat ttaaatgtca
    21001 tgtatctagg acagctgttc aaaaaagaaa cgaaaaaaag tttttcagct tatttgaacc
    21061 atttacgtat ggaaaaagcg aaacaactgc ttttgcacag taatcaaaac atcaatgaga
    21121 tagcaagtga gattggctac aacaacacca cgtatttctc taaattattc aagaaaatcg
    21181 tcggcagatc ccctaaagag taccgagaaa acagtggaga atcataaaag aaaaagccaa
    21241 tgtaatgaaa taacttataa aaaattgtct agaaatctaa ttttgggctg ttcttattac
    21301 ttgaggcaag gcagcttttt catgacctct cgataaacgg
//
LOCUS       DAZULO010000043        20826 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000043 DAZULO010000000
VERSION     DAZULO010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.044"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            591..1280
                     /locus_tag="ACG8OA_001549"
     CDS             591..1280
                     /locus_tag="ACG8OA_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4976341.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ACG8OA_001550"
     CDS             1267..2475
                     /locus_tag="ACG8OA_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS4976342.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ACG8OA_001551"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ACG8OA_001551"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFS4976343.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACG8OA_001552"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACG8OA_001552"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFS4976344.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ACG8OA_001553"
     CDS             complement(6578..7273)
                     /locus_tag="ACG8OA_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFS4976345.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACG8OA_001554"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACG8OA_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFS4976346.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ACG8OA_001555"
     CDS             complement(8472..8663)
                     /locus_tag="ACG8OA_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFS4976347.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ACG8OA_001556"
     CDS             complement(8684..9574)
                     /locus_tag="ACG8OA_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFS4976348.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ACG8OA_001557"
     CDS             complement(9561..10328)
                     /locus_tag="ACG8OA_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS4976349.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACG8OA_001558"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACG8OA_001558"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFS4976350.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACG8OA_001559"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACG8OA_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFS4976351.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACG8OA_001560"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACG8OA_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFS4976352.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ACG8OA_001561"
     CDS             14899..15282
                     /locus_tag="ACG8OA_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976353.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACG8OA_001562"
     CDS             complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACG8OA_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFS4976354.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ACG8OA_001563"
     CDS             complement(16226..17044)
                     /locus_tag="ACG8OA_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFS4976355.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACG8OA_001564"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACG8OA_001564"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFS4976356.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACG8OA_001565"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACG8OA_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFS4976357.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="ACG8OA_001566"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="ACG8OA_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFS4976358.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..>20826
                     /gene="dnaN"
                     /locus_tag="ACG8OA_001567"
     CDS             19704..>20826
                     /gene="dnaN"
                     /locus_tag="ACG8OA_001567"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFS4976359.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVR"
BASE COUNT         5940 a         4249 c         3824 g         6813 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgta
//
LOCUS       DAZULO010000044        20678 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000044 DAZULO010000000
VERSION     DAZULO010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.045"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..293
                     /locus_tag="ACG8OA_001568"
     CDS             99..293
                     /locus_tag="ACG8OA_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976360.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            562..861
                     /locus_tag="ACG8OA_001569"
     CDS             562..861
                     /locus_tag="ACG8OA_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976361.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(958..1392)
                     /locus_tag="ACG8OA_001570"
     CDS             complement(958..1392)
                     /locus_tag="ACG8OA_001570"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4976362.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1624..2334)
                     /locus_tag="ACG8OA_001571"
     CDS             complement(1624..2334)
                     /locus_tag="ACG8OA_001571"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS4976363.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2324..3184)
                     /locus_tag="ACG8OA_001572"
     CDS             complement(2324..3184)
                     /locus_tag="ACG8OA_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFS4976364.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3189..3827)
                     /locus_tag="ACG8OA_001573"
     CDS             complement(3189..3827)
                     /locus_tag="ACG8OA_001573"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFS4976365.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3842..4141)
                     /locus_tag="ACG8OA_001574"
     CDS             complement(3842..4141)
                     /locus_tag="ACG8OA_001574"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4976366.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4184..5668)
                     /locus_tag="ACG8OA_001575"
     CDS             complement(4184..5668)
                     /locus_tag="ACG8OA_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS4976367.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5688..6149)
                     /locus_tag="ACG8OA_001576"
     CDS             complement(5688..6149)
                     /locus_tag="ACG8OA_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4976368.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="ACG8OA_001577"
     CDS             complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="ACG8OA_001577"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFS4976369.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7452..8222
                     /locus_tag="ACG8OA_001578"
     CDS             7452..8222
                     /locus_tag="ACG8OA_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4976370.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8495..9322)
                     /locus_tag="ACG8OA_001579"
     CDS             complement(8495..9322)
                     /locus_tag="ACG8OA_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4976371.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9890..11125
                     /locus_tag="ACG8OA_001580"
     CDS             9890..11125
                     /locus_tag="ACG8OA_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4976372.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12124..12384)
                     /locus_tag="ACG8OA_001581"
     CDS             complement(12124..12384)
                     /locus_tag="ACG8OA_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS4976373.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12411..13712)
                     /locus_tag="ACG8OA_001582"
     CDS             complement(12411..13712)
                     /locus_tag="ACG8OA_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4976374.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14004..14462)
                     /locus_tag="ACG8OA_001583"
     CDS             complement(14004..14462)
                     /locus_tag="ACG8OA_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS4976375.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14475..15806)
                     /locus_tag="ACG8OA_001584"
     CDS             complement(14475..15806)
                     /locus_tag="ACG8OA_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFS4976376.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15817..17259)
                     /locus_tag="ACG8OA_001585"
     CDS             complement(15817..17259)
                     /locus_tag="ACG8OA_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4976377.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17246..18010)
                     /locus_tag="ACG8OA_001586"
     CDS             complement(17246..18010)
                     /locus_tag="ACG8OA_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFS4976378.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18114..18989)
                     /locus_tag="ACG8OA_001587"
     CDS             complement(18114..18989)
                     /locus_tag="ACG8OA_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4976379.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19244..19328)
                     /locus_tag="ACG8OA_001588"
     tRNA            complement(19244..19328)
                     /locus_tag="ACG8OA_001588"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19292..19294),aa:Leu,seq:caa)
     gene            complement(19346..19417)
                     /locus_tag="ACG8OA_001589"
     tRNA            complement(19346..19417)
                     /locus_tag="ACG8OA_001589"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19383..19385),aa:Gln,seq:ttg)
     gene            complement(19427..19499)
                     /locus_tag="ACG8OA_001590"
     tRNA            complement(19427..19499)
                     /locus_tag="ACG8OA_001590"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19464..19466),aa:His,seq:gtg)
     gene            complement(19561..19645)
                     /locus_tag="ACG8OA_001591"
     tRNA            complement(19561..19645)
                     /locus_tag="ACG8OA_001591"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19609..19611),aa:Leu,seq:caa)
     gene            complement(19666..19736)
                     /locus_tag="ACG8OA_001592"
     tRNA            complement(19666..19736)
                     /locus_tag="ACG8OA_001592"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19702..19704),aa:Cys,seq:gca)
     gene            complement(19782..19853)
                     /locus_tag="ACG8OA_001593"
     tRNA            complement(19782..19853)
                     /locus_tag="ACG8OA_001593"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19819..19821),aa:Gln,seq:ttg)
     gene            complement(19865..19937)
                     /locus_tag="ACG8OA_001594"
     tRNA            complement(19865..19937)
                     /locus_tag="ACG8OA_001594"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19902..19904),aa:His,seq:gtg)
     gene            complement(19940..20013)
                     /locus_tag="ACG8OA_001595"
     tRNA            complement(19940..20013)
                     /locus_tag="ACG8OA_001595"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19979..19981),aa:Trp,seq:cca)
     gene            complement(20020..20100)
                     /locus_tag="ACG8OA_001596"
     tRNA            complement(20020..20100)
                     /locus_tag="ACG8OA_001596"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20064..20066),aa:Tyr,seq:gta)
     gene            complement(20102..20177)
                     /locus_tag="ACG8OA_001597"
     tRNA            complement(20102..20177)
                     /locus_tag="ACG8OA_001597"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20142..20144),aa:Phe,seq:gaa)
     gene            complement(20213..20285)
                     /locus_tag="ACG8OA_001598"
     tRNA            complement(20213..20285)
                     /locus_tag="ACG8OA_001598"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20250..20252),aa:Asp,seq:gtc)
     gene            complement(20295..20367)
                     /locus_tag="ACG8OA_001599"
     tRNA            complement(20295..20367)
                     /locus_tag="ACG8OA_001599"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20332..20334),aa:Val,seq:tac)
     gene            complement(20383..20454)
                     /locus_tag="ACG8OA_001600"
     tRNA            complement(20383..20454)
                     /locus_tag="ACG8OA_001600"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20419..20421),aa:Glu,seq:ttc)
     gene            complement(20469..20558)
                     /locus_tag="ACG8OA_001601"
     tRNA            complement(20469..20558)
                     /locus_tag="ACG8OA_001601"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20522..20524),aa:Ser,seq:gga)
     gene            complement(20598..20671)
                     /locus_tag="ACG8OA_001602"
     tRNA            complement(20598..20671)
                     /locus_tag="ACG8OA_001602"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20635..20637),aa:Asn,seq:gtt)
BASE COUNT         6528 a         4335 c         3285 g         6530 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc
      541 agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag
      601 caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag
      661 tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata
      721 tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa
      781 aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag
      841 gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat
      901 cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta
      961 tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc
     1021 catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc
     1081 atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt
     1141 caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat
     1201 aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc
     1261 attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt
     1321 tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga
     1381 atcaatattc atttttttct tcctttctta tacaattctc cctctaataa tacagttctt
     1441 tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt
     1501 cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt
     1561 cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca
     1621 tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa
     1681 atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca
     1741 aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa
     1801 cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt
     1861 aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt
     1921 cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata
     1981 agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt
     2041 aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga
     2101 tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc
     2161 tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg
     2221 gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag
     2281 atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc
     2341 cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt
     2401 cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca
     2461 aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact
     2521 ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat
     2581 cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta
     2641 ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg
     2701 aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag
     2761 aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt
     2821 tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg
     2881 ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag
     2941 aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc
     3001 tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc
     3061 gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg
     3121 aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag
     3181 ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc
     3241 ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca
     3301 aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca
     3361 atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca
     3421 tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt
     3481 tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc
     3541 tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca
     3601 acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta
     3661 tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt
     3721 gtgcccactt caataatatc tactacatca ccaactttca taacatctcc taatgcatgt
     3781 tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc
     3841 tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg
     3901 acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat
     3961 tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg
     4021 aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca
     4081 cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca
     4141 tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt
     4201 ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt
     4261 gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca taacctagga
     4321 ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac
     4381 caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga
     4441 ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa
     4501 ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg
     4561 atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc
     4621 caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa
     4681 cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga
     4741 atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca
     4801 taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt
     4861 tgcctagtac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca
     4921 tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt
     4981 gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa
     5041 atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt
     5101 cctgcgtgat tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc
     5161 ccataaaaat caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa
     5221 aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg
     5281 tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca
     5341 tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc
     5401 caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga
     5461 ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg
     5521 ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta
     5581 ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg
     5641 tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct
     5701 gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca
     5761 tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt
     5821 gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa
     5881 cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt
     5941 tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct
     6001 acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca
     6061 agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc
     6121 aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg
     6181 gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact
     6241 caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt
     6301 taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat
     6361 cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg
     6421 actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt
     6481 caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat
     6541 tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt
     6601 ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt
     6661 cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga
     6721 ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt
     6781 gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa
     6841 ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt
     6901 ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca
     6961 tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat
     7021 gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt
     7081 tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt
     7141 ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact
     7201 tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt
     7261 ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg
     7321 tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag
     7381 atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg
     7441 aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg
     7501 aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat
     7561 ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca
     7621 attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg
     7681 aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc
     7741 tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt
     7801 tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt
     7861 atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat
     7921 taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg
     7981 tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca
     8041 acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa
     8101 agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg
     8161 aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt
     8221 aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg
     8281 ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa
     8341 tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat
     8401 caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg
     8461 gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct
     8521 ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta
     8581 aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca
     8641 atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg
     8701 ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga
     8761 ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt
     8821 tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta
     8881 agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg
     8941 catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta
     9001 aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt
     9061 ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg attttgtctt
     9121 cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa agcgcactta
     9181 agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt gcttgttcac
     9241 aaatgccttc tgctaattgt acttgtgtta accccttttt ctttcggtat tcttttagta
     9301 atgctccatc tactttaatc aaaacaattt ccttcccttt aaaaaattat ttctccttta
     9361 tatcatacac aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa taaaaatttt
     9421 aattttaaaa taatattcaa attaaacaac tggcgtatct gctccttgat ttctttttgt
     9481 gttatctgaa taattatcgt ttacagttat aacacatagt gaaaattgtc atggcgagaa
     9541 agtctaagaa tatttttaaa cataactatt tctttcttac gtgtgtataa tgacgacagc
     9601 acacctatca attaatataa ttattttatt tttttatgtt ctcatgttta tttttaatga
     9661 cacaacttct cgtatttatc tggcacaaca tataaatcga tcaaaaaatt gttgcgtgtt
     9721 tttttgaaaa ttggagtttc aacttgatta caaaatttaa atttagaagt tgtgaaaaca
     9781 gcgagagaca gtttaatatt caaaatatac ataggctact cgaacgctgt gttttttagt
     9841 atactagtaa gaaacgaagg gatagactca ttccaataag gaggatttca tgaagacaat
     9901 tctttttgct cctgcagtat tcaatttagc tgagacaaca cgaatgattg aggttgccaa
     9961 agctatgaaa gaccgttatt actgtgtatt ctttggcttt tctaaaactt ttgctccttt
    10021 gatcacagaa agtggctttg agtatcattt actagcgccg atccttacga aaaaacaaga
    10081 agaacaaatc ataaaatttg accagttgcg tacacttaaa aaccctttta ccaaagaaat
    10141 ggttgcctct cgtatcacta gtgaacagag tctgattgct aagtttgagc ctcgactgat
    10201 tgtgacaggt tcgaatgtga caattttttt atcggcccgc aataaaaaga ttcctttagt
    10261 atatgttaaa ccctatgctt tgagtgaacc tttttttcaa tcagagcatg cgctgatgcc
    10321 ccacgagtta cgtttcttcg gccctttaag caatcccctt tggcactgga caaaaaagcg
    10381 tatagtaaat acccgatgga tccccaatgc cttcaaacac gctgcacaaa aagaaatgct
    10441 tcctttacca aaaacaagtt tacaaatgat ggatgctgat ctaaatctca tcacaacgcc
    10501 taaactattt ttggaagaag aacagctgcc ggaaaattat cgaatcgttg gtccaatttt
    10561 tgctaagctc agtacttcct tgcctgaaga agtgctaact tttatacagc gaaagcaaca
    10621 agaaagaaaa cgtattatct atttcgcaat gggtagttca ggcaatccgc gtatgttacg
    10681 caaaattttg ttcttcttac aaaaacaacc ggatctagcg gttattgcac ctattgacta
    10741 tctgcttgat gacattcaat ctgactcaga agatctttta ataacagaat atctgtctag
    10801 tcatcttttg catgaattca ttgatttttc ttttatccac ggcggggaag ggaccgtcca
    10861 aactgcctgt gaatccggca aaccgtttat cggtgtgggg atgcaatttg agcaaagctg
    10921 taatattgac tattgtgtaa aatacggaaa cgcaatcgcc ctcacaaaac cagtgactga
    10981 aagaaaactg cagattgctc tgcgcgaagt ttcttcaccc aagattcgtc agcaggcacg
    11041 cacgctgcaa gccgtttttc caataaatgg tccacaacaa gcaattcaag aaatcgaaac
    11101 cttcctgagc aaccacccaa tatagtttca agtagaaaaa gcttttttaa tagagactca
    11161 tcagtaattt cgctattcga gacccagaca gtgaaaatac aaaaaaagta aatagtggat
    11221 tgaaaagtta gagttactta aattacggtg tctaaaaaac acaaaatcat ccttaaaata
    11281 aaagatctga cgctatccaa atgagcatag gtagcgtctg ccatttattc ttagtcaaat
    11341 tctttgacac ctttctctgg ttggatcatc tcaacgtttc ttcaaaagaa agaccataat
    11401 aattaggcaa atacattttc ttaaaatgtg tcatatagat ctagaagcat cgctttgtag
    11461 agatttgtgc cgctagtccc ctcaacaatt actaaatcaa atgtaggatt taccacttta
    11521 tctttgataa tcaacctccc tcttctgttg agacaatgat ctcgcaatcc gatgcatact
    11581 cctatataaa ctgccagcaa tccccacata ttgagtagat catgtcagaa tgttctatgt
    11641 cgtcagcaac gatacactaa aagtcacgtt caccatgact gattgctatt cccagcacaa
    11701 ttatttctat acacgtttca tgatttgaat aaacactcac atcatacata ttaccattca
    11761 tacaccatac tgcagcccct actatatggt tgtaagctcc caaatctata tttgacttga
    11821 tcactttggt agtagatcta ctaattctaa atccattgct gataaaattt ccattttttc
    11881 ctccttcttt ctgccaaaac agttaaaaca ataataatct ttatgtaaaa aagggctgtg
    11941 acaaaagcct tgtcacagtc ccatattccg aataaacggt ggtcaaaagc ttgcatctgc
    12001 ggtaataagt tcaaccaaac gaaaaataag ccaacatttc acaaaaattt aagaagcaat
    12061 tttcgtaaat ttcttcttat ttcttgaagc taaccacttc tgttacaacc acttttagaa
    12121 catttaaagc ttcgatgtga caaaccatct atgctgctcg atcataaaat tgatagcaaa
    12181 aattccacaa agtacatatg tcccccatga aaacaaaaca ttcatatgcg gtacaatgat
    12241 ctttggaaga atcacgatca aaaatgatgc cagtgcccaa atccatcgat tgatttgaat
    12301 atgatatgta gctagcatat tcaatataag gtgactagcc gttaataaaa agataaaaac
    12361 aaaaaataaa atcagccaat acattgtttc ccaccttcta aaaaccaaac ttagacagtc
    12421 tggacttcgt tttctccacg actttgttca tctgacagca tctttttatc ataagctttt
    12481 acaaatggat aatagataat aaatgctatc aatgtgttga tcatgatcaa gataacaaca
    12541 cgccagtcat ttcctgctga aagataaccg ccgattggag ctggtaatgt ccatgttggc
    12601 aaaacagtaa atctattgac catttctaat ttgactgcaa aataactgat aatagtcaaa
    12661 actaaaggaa ctaagttgaa cgggatgaac atgtaaggat tcaacatcat aggagctcca
    12721 aaaattatgg gctcattgat attgaagata ccaggaataa tagaaaaacg accaacctct
    12781 ttcaagaatt ttgatttaca gaagaatata aacaaaacag ataccacgat cgttgctcct
    12841 gcaccaccaa ttgttaccgt ccattgataa aactgttcag gagcgatata gggtaattgg
    12901 gtggcagctg taccatctgc caatgccttt gcattctcat ccaacatgat cagccacatt
    12961 ggacgaacaa cagcccctac aatactcatg ccatggatac caaatgacca caacaaatgg
    13021 atcaaaaaca ttggtagtaa aacaccaagc aaattattcc ctaaaatatt ggttaaaggc
    13081 tcgaataacg ataataaggc agcgtttaca tcaaagcccg cgacgcttcg aatcagccaa
    13141 gtaattgtta aaatgactgc acctggaatc aatgcctcaa aactacgggc aacagcaggt
    13201 ggcacttgtt ctggcatttt gatagtaata tttcgatttt taaagaaacg atatacttcc
    13261 actgcaaaaa tcatagataa gatccccgca aacatcccag atgctcccaa agaactcata
    13321 ggtaagaccc agccaagagg tgcctcttca ggagttaaaa cgtttacagg aacttgtaac
    13381 ataaagaacg ttcctaaaga taatatacca cctgtcacac tatctaatcc atatgattta
    13441 gctagatccg aacccataac aaaagttgca taaatagcca tcaatcccat cgtcatccta
    13501 aagggtaaaa caatcgttgc cgaataaggg gcaataaatt cattccatcc aggaaacggt
    13561 aaattgataa agaccataaa aaatgtccca atcaaaatca atgacaatgt tgaaacaacc
    13621 ccattacgaa ttgctaataa atgacgctgt gtaccaattt ttgccattgg tgggacaagt
    13681 ttctcttcga cccatttcat cagcttttcc actaaaacca ctccttttca ataaaatatg
    13741 aaccattcat tgcatgacaa cgcaaagaat gttcataact aattatataa ttaagaaatt
    13801 ttatttcaat atattctctt aaataatcaa atttattttt aagaatcctg aaaataagtt
    13861 ttctaaatca taggagcaac aaaaaaattc gataattaac aaaaatcaaa atgattaaat
    13921 ctagtgattg aaacatatca tttcaaaaaa attttgtaaa ttcacccata aatatcaccc
    13981 tatttaactt ttttcatcgt acactaaaat tcaaaacaac tatctttaat ctttacttct
    14041 tggttttgtt ttaataaatt tccttcaaaa atgcctggag aaactacaat gatcaccgta
    14101 tctttttgtg cttgctccaa cttcttcaaa tctataatag ctaacagtgt cccttctttt
    14161 acctcctggt tactttcaac ccgtatctga aacggacttc cttcgagttc aactgtatct
    14221 aaacccatat ggatcagtat ttttctgcca gattcacttt cgatcgttat ggcatgtcgg
    14281 gtaggaaata tattatcgat tattccttta actggcgcat ggatatatcc atcatgattc
    14341 gttatagcgt atccaactcc tatcattttt gaagaaaaga cctcgtcttt tactgctgtt
    14401 aatggaataa tttgaccact agctacagct tttagaatgt ttcttgtttt aaagaaatgc
    14461 actttttatc acctctactt taatgctggc caatattttt tgtttacttt gatcatttca
    14521 tctagtattt tttttgcgac tctggcattt ggtacggttt gattcaatgt aaatgcttgt
    14581 aaagcttttt gataagaacc ttcaaagtaa gcatctacta gtaacttctc tactgcaact
    14641 tgtgcctcca tcaatccttt atgaaaatca ctgattggtt ttcttagtgt aattggctcc
    14701 acaccagttg caccaacata tgccggtatt tcaacaactg catctgaccg tagattcgga
    14761 atcgcccctt gattaggaac gattagcata aagcgacgac gatcatcatt caacaaggaa
    14821 atagctatat cgacgatata ttgtccatgc tccccaaaat taaagttcaa tacttcttcc
    14881 acaataccgc ggcgtatttt atcagccatt tctttggtat ttttctctcg tccatccatg
    14941 accatattag cacgtgtata ctctttgtca gtatgctcaa cgaccatatc aggatataag
    15001 taatactcta agtaattatt cgggatatta cttggaaaat ttttcgtgat gactgacatc
    15061 atttgaaagg ttttttgcca agttttatct cctatattaa aatcggcgac ttgcatttct
    15121 tttgttatta gtttttcaat aatctctggc ataatttctc gttgcaattc tttatcataa
    15181 atcgatgtat accagccaaa gtggtttaaa ccataatacg taacgatcca attcttacga
    15241 tcataaccgt aattcactgc aatcgtttct tcaattgaaa tagtcatatc acaggcatta
    15301 atcattttta cattcggata tttacgacga acagcttcag aaacgatcga ctctggattt
    15361 gtataattca aaatccatgc tttgggggca tagtcttgta tatgatcgac aatttccaat
    15421 aaacctttga ttgagcgcat cccataagca aatcctccga gtccacacgt ttcttgtcct
    15481 actaatccat gtttaagtgg aatcttttcg tccttttctc gcatctccaa tcctccaaca
    15541 cgaatttgtg agaaaacaaa atcgcatcct gtaaaagcaa gctttggatc atccgttgca
    15601 cgaatgcgaa tatttgaaaa accttttctt ttcagcataa atttaacaat caagtacata
    15661 tccatatttc tttcttcatc aatatcatat aaacgtattt cagataatgg caaccgtttt
    15721 tcattgttca aaatggcttg aacgattcct ggtgtatacg tactcccgcc tccagctata
    15781 gtgataatct gttttttcat catcatcttt tcctttctat tcttctaata atttattgaa
    15841 ttctgccata acataagtca cttctaagcc gatgataatt tgataatttt tttcagttgg
    15901 tttaatgatc ccgcttatcc ctaattgctt gattcgctgt tcctcgacca tggtgggatc
    15961 cactacctct acccgtaggc gtgtattaca actatagaca tccaggatat tggcttttcc
    16021 tccaaacgct tgtagcattt ttgttgccat atattcataa tttttacttg ctaaccctaa
    16081 ctctgtttct tctaaggtta cttcattgcc aaactctgcc ttagcttcac gtcctaatgt
    16141 ctgaatgttt ctcttattga tcaaataata aaaagtaacg taatacaagg caaaaaagaa
    16201 aattccgatt ggaagaatca aaatagcatt ggtagcgatt cgaaaattga tgagataatc
    16261 aacaatacat gctccgaatg taaaaccaat tcgtatattg aataagtaac agacaatccc
    16321 tgctaaaccg gcaaaaagtg catggatcac gtacaacata ggagaaagaa acatgaaact
    16381 aaattcaaca ggctcagtga tatttgcaat aaatgaagtt cctgcaccgc ttcccatgat
    16441 tcctttgacc atctctttat tttctttata agctgcttta tagatagcta gacatatagc
    16501 tggtatacca aacatcatta ctacaaaaaa aactgataaa aacagtccag cctgaggatc
    16561 tccgttgata aagcgtgtca tctctcctct gaaaacttcc ttgctaggag atgtatagct
    16621 tcctaaatcg taataaatat acgtattcaa cacatggtgc agtccaaagg ggatcagcaa
    16681 acgattcaaa aacataaata tccccacacc taatgctccc atatcaacga gcagttttgc
    16741 aaaattatca atcccaattt ggatgagcgg ccagataaag cccaaaaaaa cagctgcgat
    16801 tgtttgtaga atcatcacca tagtcaatgg gaatttttcg cccgcaaaat aagaaaaaac
    16861 tactggtaat ttttgttctt taaagcgatt atacgtccat gccgctacta aaccggaaat
    16921 aattccacca aaaatcccca tattcacttc tgggtccata atttctaagc cttttttcaa
    16981 ggtagcaatc gctaaaaaac cagtaagtgc aggtattcct ttgtccttat ttttgacaaa
    17041 accgattgca actcctatag caaataagat atccaagttt ccaaaaacgg catttccagc
    17101 tgcaccaatt actgggatgt tcagcatatc gtcagctgat aaacgcatga tcaatcccgc
    17161 taatggcatc gctgcaactg gaagtagcat cgctcttcca agcttctgca catttgattt
    17221 aacattcatt tttattcctc ctctattaaa tatcctcata tgttactaac tcaatcaaag
    17281 gatcattttt cactttttga gcaaatgctg aatgaattaa aaattcgact tggtgggtac
    17341 gaggatacac aaaacttgaa ccatttaaca attcctgatc caaatacgct gcgtgacaca
    17401 tgacttcgat cgttttatct tctctcaata tcatgagtaa acttgccaaa tattcttcca
    17461 cactcgtatt gatttgtcgt tttttttcat cagtttctcc tacgacatcg aaacaaggct
    17521 caaaataatc gacatgacga acttcatctt ttcgttcaaa gttgctccga accggcaaat
    17581 catatttttt tgctaatgta acaactacct cctgattttc atccaaagta tgagtatgat
    17641 gatgactgtc taaatgagtt ggtatgatcc ccgcatgata aattttttga atctgagcat
    17701 tccattcctg atacagttga tctttgtcaa tgttaaatgg atgctgataa taagataagg
    17761 atctgaactc tccgctttcg ataagagtat cgacagtttt gaggagtggt ttgccacacg
    17821 tcaatgttaa atggactcct acacctagcc taggcatttc ctttcttagt ttcacagcat
    17881 gctcgaaacc cggcatattt gccatcaatg tagttgatgt caaaattcct cgctgataag
    17941 catccataat cccataattc acaccatggc tatagccaaa atcatccgaa ttaaaaatca
    18001 cttttcccat ttcattctcc ttcttttttc tactaaaata ggcgcaacca aataatcata
    18061 gtccatttac tatgtgtcat ttggttgcgc ctatagatag ttacgccctt attttaatag
    18121 attcttgcta gatgaattgc caaatagatg atttcatttt cttgaatggc aagttgaaat
    18181 tgctgttcga tgatatcttt aatctttagt gctgtacaat acgctttgct atgtttaaat
    18241 gtttcagtaa aaaaaccaat attttctact ggcgtttctt cctgataaat caagcgttta
    18301 accaggaatt tgatatgaac cattaagcga tgataagtca gtgattcttt atcataaacg
    18361 tttgaatagg atttttctag atgggacaaa atcgtggtcg tcatttctac tatttttaag
    18421 ctctttaaac tattaggctc tcctagctca gcattaatga tatgcatagt gatgaatcct
    18481 acttcatttg accctaactc ggtttgatat cgatgattga ttcgctcgac cacttctact
    18541 gctatatcat attcatttgg ataaaggaat ctcatttctg gcaacataat tgctgtgaca
    18601 tctctatctt ctctttttcg ttgaatagca aaataaatat gatctgtcaa tgtaatatag
    18661 atatttgaat ctaatggaga ggagatccta gctgttatca gttcaatcac ttcttccgca
    18721 aaatccataa attcgattgg aatctgcgag actatctcac tgaatcgttt gatcttttct
    18781 ttagaatcta atacaaataa tttgtcagca tcctcaacat caaatggctt actcacttta
    18841 gcagaaaagc caatcccttt ccccatcgca attttttcta ttccatcgtc atcgatcaat
    18901 acagcattat gattcagctt tttcatcaca tacaccgtta attcacctcc tctatatgat
    18961 aaaacaaaac aacttgcaga ctccttcata catatttttg tctccaaaca atttttaaag
    19021 acaaaaaacg acctaagaaa gaaccctttc ttaggtcgtg cctaattgaa tagtagcacc
    19081 ctacataatt tacaaaaata gtttaacaca taataaaagc gttatcaata tcttttttac
    19141 tcgttttatg ataatacttg tatcgaattg atttggaggt aattacataa aaacaaaaaa
    19201 aatatagcaa ctctctataa gaaagttgct atattgataa ttataccggc ggccggggtc
    19261 gaaccggcac gtcctcaacg gacactggat tttgagtcca gcgcgtctgc caattccgcc
    19321 acgccggcaa atttaaaaat aataactggg gtagctggat tcgaaccaac gcatgaggga
    19381 gtcaaagtcc cttgccttac cgcttggcta taccccaaga ataaagggcg aatgatggga
    19441 atcgaaccca cgaatgccgg agccacaatc cggtgcgtta accacttcgc cacattcgcc
    19501 atcataaaag atgttattta atttctcaat aaaaaactaa gatgaagaca tcttagtcaa
    19561 taccggcggc cggggtcgaa ccggcacgcc ctcaacgggc actggatttt gagtccagcg
    19621 cgtctgccaa ttccgccacg ccggcaagtt gatttattag tactaaggcg gtaaccggat
    19681 ttgaaccggt gatgaaggtt ttgcagacct ctgccttacc acttggctat accgccatta
    19741 tactattatc aatctataaa ataaagaata aattatagaa actggggtag ctggattcga
    19801 accaacgcat gagggagtca aagtcccttg ccttaccgct tggctatacc ccaagaataa
    19861 aaagggcgaa tgatgggaat cgaacccacg aatgccggag ccacaatccg gtgcgttaac
    19921 cacttcgcca cattcgccat ggcaggggca gcaggaattg aacccacact aacggttttg
    19981 gagaccgctg ttctaccttt aaactatgcc cctataaaat ggaggagagt ggattcgaac
    20041 cactgaaccc taaggaacgg atttacagtc cgtcgcgttt agccacttcg ctactcctcc
    20101 atggtggcgc gggacagaat cgaactgccg acacacggag cttcaatccg ttgctctacc
    20161 aactgagcta ccgcgccata ttttaaaatt attaattgaa taacaagaac gacggttccg
    20221 acgggaatcg aacccgcgat ctcctgcgtg acaggcaggc atgttaaccc ctacaccacg
    20281 gaaccagttc attatggagg ttaacgggat cgaaccgctg accctctgct tgtaaggcag
    20341 atgctctccc agctgagcta aacctccaac attcaattaa aatgacccgt acgggattcg
    20401 aacccgtgtt accgccgtga aagggcggtg tcttaaccgc ttgaccaacg ggccaggtaa
    20461 atttgctacg gagagtaagg gattcgaacc cttgagacag tttggcacca tctacatgat
    20521 ttccaatcat gctccttcgg cctctcggac aactctccag agtgaaagaa gctcaatact
    20581 ctttctcaaa atgagaactc cggcagtagg actcgaacct acgacatcat gattaacagt
    20641 catgcgctac taccaactga gctatgccgg aataacat
//
LOCUS       DAZULO010000045        20586 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000045 DAZULO010000000
VERSION     DAZULO010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.046"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="ACG8OA_001603"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="ACG8OA_001603"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFS4976380.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="ACG8OA_001604"
     CDS             1011..2258
                     /locus_tag="ACG8OA_001604"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFS4976381.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="ACG8OA_001605"
     CDS             2334..2678
                     /locus_tag="ACG8OA_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976382.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="ACG8OA_001606"
     CDS             complement(2795..3121)
                     /locus_tag="ACG8OA_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976383.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3736..4938)
                     /locus_tag="ACG8OA_001607"
     CDS             complement(3736..4938)
                     /locus_tag="ACG8OA_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4976384.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            5055..5570
                     /locus_tag="ACG8OA_001608"
     CDS             5055..5570
                     /locus_tag="ACG8OA_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4976385.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5769..6215)
                     /locus_tag="ACG8OA_001609"
     CDS             complement(5769..6215)
                     /locus_tag="ACG8OA_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS4976386.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6262..7740)
                     /gene="msr(C)"
                     /locus_tag="ACG8OA_001610"
     CDS             complement(6262..7740)
                     /gene="msr(C)"
                     /locus_tag="ACG8OA_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS4976387.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(8094..8723)
                     /locus_tag="ACG8OA_001611"
     CDS             complement(8094..8723)
                     /locus_tag="ACG8OA_001611"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS4976388.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(8707..9171)
                     /locus_tag="ACG8OA_001612"
     CDS             complement(8707..9171)
                     /locus_tag="ACG8OA_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4976389.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9658..10926)
                     /locus_tag="ACG8OA_001613"
     CDS             complement(9658..10926)
                     /locus_tag="ACG8OA_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS4976390.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(11157..11894)
                     /locus_tag="ACG8OA_001614"
     CDS             complement(11157..11894)
                     /locus_tag="ACG8OA_001614"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4976391.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11961..12530)
                     /locus_tag="ACG8OA_001615"
     CDS             complement(11961..12530)
                     /locus_tag="ACG8OA_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFS4976392.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12658..13530
                     /locus_tag="ACG8OA_001616"
     CDS             12658..13530
                     /locus_tag="ACG8OA_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4976393.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13872..14744
                     /locus_tag="ACG8OA_001617"
     CDS             13872..14744
                     /locus_tag="ACG8OA_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4976394.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14741..16699
                     /gene="nagE"
                     /locus_tag="ACG8OA_001618"
     CDS             14741..16699
                     /gene="nagE"
                     /locus_tag="ACG8OA_001618"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFS4976395.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(17031..17882)
                     /locus_tag="ACG8OA_001619"
     CDS             complement(17031..17882)
                     /locus_tag="ACG8OA_001619"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4976396.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            18005..18472
                     /locus_tag="ACG8OA_001620"
     CDS             18005..18472
                     /locus_tag="ACG8OA_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFS4976397.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18735..19217)
                     /locus_tag="ACG8OA_001621"
     CDS             complement(18735..19217)
                     /locus_tag="ACG8OA_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS4976398.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(19317..20180)
                     /locus_tag="ACG8OA_001622"
     CDS             complement(19317..20180)
                     /locus_tag="ACG8OA_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS4976399.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         6197 a         4227 c         3724 g         6438 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgat cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca atcagctgac cctcggtatt agctcaaaaa acggtaaaat
     3421 atgagaagct atttttccgt tttgtttctc taatacttag gataaaacgc tgattttaca
     3481 acctctgtta aacggtgttc aatcagctgt ccttcggtat tagctcaaaa aacggcaaaa
     3541 tatgaggaac tatttttccg tttgtttctc taatactcag gacaaaacgc tgattttaca
     3601 acctcctgat aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa
     3661 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     3721 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
     3781 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
     3841 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
     3901 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
     3961 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
     4021 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
     4081 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
     4141 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
     4201 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
     4261 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
     4321 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
     4381 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
     4441 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
     4501 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
     4561 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
     4621 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt
     4681 caaaccgatc cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat
     4741 gtaactagta aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt
     4801 ttttaagatt gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac
     4861 aactagcgct gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg
     4921 attctccaat atttccatcc ctttctctct attagcatca tacatgattt attttacatg
     4981 tcaatatgca catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga
     5041 gaggtaggat ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac
     5101 cacgttctgg gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc
     5161 agatctatac aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag
     5221 acgagcactc tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg
     5281 aatggatcaa agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct
     5341 atgctttttc gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac
     5401 agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga
     5461 aacaagaatt tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac
     5521 tccaatggtg tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca
     5581 gaacctttct agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt
     5641 tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt
     5701 ctagaactgc attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca
     5761 gtgttccatc attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg
     5821 atgatcaact gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca
     5881 gagaataatt gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc
     5941 accattccta aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga
     6001 tttactggta gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa
     6061 atgaaagcca ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct
     6121 tgatgcagcc ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt
     6181 gtaaaaatta ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc
     6241 acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc
     6301 ttttatttca taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa
     6361 caaaatcgtt cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc
     6421 aataaaatta gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc
     6481 aatcgtcaga cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc
     6541 ctgagcaaac cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg
     6601 tctcatgaca aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata
     6661 tacttgaaag cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc
     6721 taataaggtt gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga
     6781 aaactgaaaa tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg
     6841 tcccatgatg ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg
     6901 tttcatctgc tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt
     6961 cgcattcttt tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc
     7021 cagtctgatt tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt
     7081 tttcttttct tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc
     7141 tcgtagctga gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga
     7201 aaactctcgg atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa
     7261 atagcgatca tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag
     7321 tcgatcaata ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc
     7381 ataagaagaa agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg
     7441 ctctgaagtt tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt
     7501 tgcttcagcg agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt
     7561 cccatgatcg ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc
     7621 attttctcct atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag
     7681 ttctagctgg ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat
     7741 atgttttaca ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa
     7801 aatctgtttg caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac
     7861 taaatcacca aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat
     7921 actccatcca tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa
     7981 aagttcgaaa cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt
     8041 accacacacg attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg
     8101 aacatagtga tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt
     8161 cctattgata aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc
     8221 aactgatctg cttcgaatcc accaattgga tttgtttcat agccatgatc acgcgcaact
     8281 aacatgaatt gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct
     8341 cttgaaaaac tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt
     8401 ggcatcttcc ctgattcgta agcttggttg tagatttctt cgccatattc gtaacattgc
     8461 atatcgccaa aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg
     8521 atcaaaggac gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt
     8581 ttcatattga cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg
     8641 tgtgggattt tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt
     8701 gaaaaatcat ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca
     8761 aagttgggta ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa
     8821 agccggggat agcacggaac ctgtatcttt atggtccatt tcacaatgct tgattttctt
     8881 ctgggctttt tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg
     8941 cgaaacatag ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat
     9001 ctgcaacttt tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat
     9061 gatctcgtat ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg
     9121 atcggctaat ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc
     9181 ctttctaata attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa
     9241 agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag
     9301 aaattttttg tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat
     9361 tattgattct tatcaaacta tagaagatag ctcttctgca gtagataatt ttacaccgcc
     9421 aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta
     9481 tgcttaaaca gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac
     9541 ggtgttcaaa aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct
     9601 gtttttgttt ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta
     9661 ataaccataa aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc
     9721 cttagcactc aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata
     9781 aattggcttg gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt
     9841 taaagagttg atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta
     9901 taaacggtag acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca
     9961 gcctacagtt ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt
    10021 atgacggtta tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt
    10081 cagattatag gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata
    10141 acgacctgtt agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc
    10201 ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg
    10261 caatgattct ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc
    10321 attcacatat tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc
    10381 aaatagattg taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat
    10441 ggcttgagca atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt
    10501 tgaggcaata ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc
    10561 atagccagta tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg
    10621 cccatctgtc aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc
    10681 tgatgtactg tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga
    10741 aattgtattt tctgagacag tagctgactg caaagtactt tctgtatcgt tttcaattcc
    10801 tgtttctgaa gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac
    10861 tgttgtgttg gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc
    10921 tttcatcttt tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa
    10981 agtaaaagca agtcaatgtc catatttaaa taacatttgt ttctttttaa taaatttgct
    11041 ttttctgcat aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat
    11101 ggtcgtttga ataactattt aacaagtaat tctctctgga tattatccgt tatggattat
    11161 acttcctgtg tcgtcggacg gataatcatg ttgctgacag acatccggtc tggcgtttct
    11221 atcgcaaatg ctacagcaga agctatatct tcagatgtta atccccattc tttttgcgct
    11281 tcatgaagtt caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca
    11341 gctcctggag agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct
    11401 tccatgattg cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca
    11461 acatgtcccg ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt
    11521 ggaagtacgg cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc
    11581 cattcatcac ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac
    11641 aatacatcga ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca
    11701 aaatctctta catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt
    11761 gcttttaggc gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat
    11821 agcttcgttg ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct
    11881 gataaatcat tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat
    11941 ctcttatttc atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg
    12001 attcctgcaa cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca
    12061 atggcattct cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca
    12121 tttttcttga tgtggattgg ctttactgac aagcctcttc gttcttttgg atcggttaga
    12181 tggttcaccg tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg
    12241 ccacctaagt caacgaacat cacttgacga ttgataaaga catttttccc tatactaata
    12301 tgtttgccga aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca
    12361 ctaatgatat tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc
    12421 gctgctaaag gctcttcgtt ttccgcttgg atctgatgga tcacttcaaa gagcggatcc
    12481 gttactcgaa tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct
    12541 ccttttatcc ttactttata acggacggct tatgatgtct aatacttata aaacatactt
    12601 aactattcct tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg
    12661 gatattcgta tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca
    12721 gcaaaatctc tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa
    12781 gaacttggtg tgacactttt cactcgaggc tcccgagaga tacagttgac ggattctggt
    12841 cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat
    12901 ttaacaaatg acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca
    12961 atctctttca tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt
    13021 catttatata gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat
    13081 tttggactcg taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc
    13141 caagatcgtt ggggactttt gatacatcgg aatcatcctt tagcaaagca aacagtcatc
    13201 catccgcagg aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt
    13261 caactagctg aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac
    13321 ttattgtaca atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat
    13381 gggatcatca atacttatgg gacggaaaaa gtattcatcc cactcgatcc gccattacaa
    13441 gcttcactga atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt
    13501 ttagatgtcc taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt
    13561 ttaacaacga ataagttaag caaaagatcc aatgattaaa taacataagt ttcttaattt
    13621 gttccttcct tttatttctg ataattccag taaattacta taattataaa aaaagaattg
    13681 acaaaacata actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact
    13741 agatcatact aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg
    13801 tctatttttg tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa
    13861 agggtgcgcg catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg
    13921 gtggacagga aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt
    13981 tgatttttga tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt
    14041 tgcaaaattt acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg
    14101 atcatgcgga aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg
    14161 accacattgc tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt
    14221 taagagaaat cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt
    14281 atctgacaaa agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc
    14341 atattcacag cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga
    14401 ttgtatctga catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac
    14461 cattttcact gaattattca agactggtga atcatttacg actgcttcta caacgaaccc
    14521 atgcgcaaca atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc
    14581 cagaaagcta taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg
    14641 cgatcacgaa agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa
    14701 tcgtacaaac aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg
    14761 aatgggacgt tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg
    14821 gatcggaaac tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg
    14881 cggtaacgca gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat
    14941 gtcagtggac aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt
    15001 aaatgtttta aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga
    15061 tccatctttt gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc
    15121 agctctttat aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg
    15181 caagcgtcta gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact
    15241 gctcgtatgg cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt
    15301 aggctggggt ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt
    15361 gcatcaagca atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa
    15421 atttttggct tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc
    15481 aatcatgatg ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga
    15541 aaacaaagca aaaacagctt ccttgatgtt agcagcagct tttgcttcat tctttactgg
    15601 tgtaacagaa ccacttgaat tctcatttat gttcgttgct tggccattat atgtcttgca
    15661 tgcaattttc actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt
    15721 tgcctttagt gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca
    15781 gccattgatg ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa
    15841 ctttgctatc aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga
    15901 tgacgatact gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta
    15961 cgctgcatta ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg
    16021 tttgcaagtc aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc
    16081 tggtatgaag atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt
    16141 cgttgcagat gagatggcaa aactacatgg tggtgctgca acacgacctg cacaaacaaa
    16201 caccgtggtt aaaacagaaa catttgccgg tggaacgatc gaacaagata tttacgctat
    16261 cgcaaatggg aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat
    16321 gatgggtgac ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg
    16381 tacgatcaca aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat
    16441 cgaagtcttg cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt
    16501 atatgtaaaa gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc
    16561 cgctattcaa tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa
    16621 agtccaaagc ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc
    16681 tgtttccaac aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag
    16741 gagctgagac aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc
    16801 caactccgcg gtaataagtc cagccaaacg taacatatga agagctattt tttatttgac
    16861 tcttcttatt acttgatgca gaacagcttt tttacaacct ctgataaacg gtgttcaaaa
    16921 accagtttat cggtatgaag tcaaatttat gcaaaaatgt ggaaagccat tttcacaaaa
    16981 tttttcttca tactcaaaac taatcggctt tttcacaacc tctttataca ttattcctga
    17041 tccaatattt cttttaatgc tgcttcaaga gaagttaatg gttttcccaa tactttttct
    17101 aaatcatcag aggaaatagc taactgatcc tttttaatat catgttggat cgctaaaaac
    17161 atgtcagctg cttcttgagg gaatccggct tcttttagat tttcgacaaa cgctgcatca
    17221 cttgcttcaa taatttctaa ctttttacct gtcgcacgct ctaatgcctt ggctaattct
    17281 tcatagtcag ttagattgcc ggataattct aagatttctg gagagtcact ttctactagt
    17341 acttttgctg cagcttctgc atattcacgt ttcaatgccc aacctgtttt tccatttcct
    17401 gcagcataaa cgaattgacc agtttttaaa gcctgcccaa tgataggtaa ttcattctct
    17461 acataccaat tattccgtaa aaatgtatgg gaaatacctg aatccttaat cattttttca
    17521 gtgaattggt gatctggcgc taatacactg atcgagtgat cggcatctgc aaaacttgta
    17581 taagcgatat aagatacccc agctgcttgt gctgccttca ctacattctt atgttcggct
    17641 tgacgatttc ctggaacgcc agagacaaaa agcaatcgat ctattccttt caacgcttct
    17701 ttcattgagt ctaaatcatc ataatcacca atacgtatat taaatccagc ttctttcagt
    17761 gcttctcctt tttcttcact acgtactagt acatagattt cattttgtgg aaccatcttt
    17821 tttaagaatt ccagcgcata accaccaaaa cctccagttg ctcctgttac taaataatgc
    17881 atatttgcca ctccctattc tttttatttg ttaagattta cagctgtaca tttataaatt
    17941 acacctttat cataaagagt gtggtaagat aagtcaagca aaaaaaatga aaggatgaca
    18001 actgatgaag tattctctgc aatttagtga tgcaatccat gtacttgcct atattgaaat
    18061 gtttcaagat acccgatcac tttctagtga gatgattgcc agtagtatcg aaacaaatgc
    18121 agcaaatgta cgtaaaatca tgagtcagct caaaaaatcc ggtttgatct ttacaaaaac
    18181 cggaaaaccg cagccttccc ttgccaaaag tccagatcag atttctttac tagatgtcta
    18241 tcgcagcatc gaaggtaata cacagcttat ccaagtcgat ccaaaaacaa atccaaattg
    18301 cgtcgttggt gcgaatatcc aagaagcttt agaagaaagc tacgctcggc tgcagaaaaa
    18361 ggcagaagaa gaaatggcta agatcacact tgatagtctg atacataaaa tagccgtttt
    18421 ggaaaaagaa aaaagaccag aaaacacagg cctcgtagag agatttttat aattctctct
    18481 actgtgcctg catttttctg atcggaatcg tctacataaa ttcttttatt ctccggggga
    18541 caaaagcctt gtcatgcccc tttttcctct ctaatgctca ggtcaaagcg ctgaagtcat
    18601 gacgtcttga taaacggtgt tcaagtagct gaccctcggc attagttctg aaaaatgcaa
    18661 aatatggaaa gctatttccc atttttcctc tctaatgctc aggtcaaagc gctactttca
    18721 cgacctctat attatcatgg tttattgact accggtatcc agatttctcc catatatttc
    18781 ccgttggtaa ctcccatttc gactacagca tttggacccc caacataggc gtaatcttct
    18841 acttctctta aaacatgccc aaaggccgta ttcgtcaaat catttcttaa ttcttcaggc
    18901 gattctgcta aagcgattac aacaagatac gttccttttg ggaatgggat gattcgggta
    18961 gtgtttggaa catcctgact cgtttgaaca ccgacatagt acatcatttt cccgttatag
    19021 ggttcgttga ctgtaaagaa ataatgattt tcagcagctt gtttcaatgc atctattgtt
    19081 ccattaccga taactttttg ctcaaatgct tgtttctctg cagccaaacc gacaaaatcc
    19141 gtattgctac tcttcaattc cacaccaata cccgtcaccg taaaatctgg tttttcttcg
    19201 atcctatact ttgccatttc tcccaccctt tctattatat taagttcatc ttacataaaa
    19261 ttaaagggaa atgacacgat tacgctcttt tttctaggga tatttttatt ttttaactat
    19321 ttttttgctt tcactgcaat ccaaatctca ctgtaaacat tactgaaatc ctctccccat
    19381 tttgtaaaag agatctcggg agcttcgact aactcataat ctgaagaagg aagccattct
    19441 gcatagattt tcccccaagt atcttgtaaa gtttggggaa atggcccttt attaggaaaa
    19501 atggcccaag tgtgttcttc tatagcaagc atttctaaat cagcgtacgg attttctttt
    19561 gtcgtagcaa agccaatcat gtgcatcaaa ctgccctttt cttccaagcg ggctccatca
    19621 aatccataag aaacgttgat tacttgattt ggatataaat ctcctaactt atgcatctct
    19681 tccctttgag tctccgtgat cgtctgcgct aacttttgta tttcttgatt gactccttca
    19741 aatttgacag gaactttttt cgccacacct gccacataaa actgttcttt cttttcgata
    19801 cgaaattcca tcgattttcc tcctctgaca tcaataaaaa atgtcaagcg tggaaaagat
    19861 ttttgtatgc cgtatctgct gatttctgaa ggcagatatc ctgaccaatc acgaaaagct
    19921 cttgaaaaac cttcgactgt ttcatatcca tatctaaacg ccgtttctgt cactcttgat
    19981 ccatctagga gatcgcgatt agccaaagcc aatctacggt ttttgatata gtccattagc
    20041 gacatccctg caataaacgt aaagatgcgt tttaattgat attcagagat tcctgtattt
    20101 tcggctatta ttttatagga aagtccttct gtcaaatgtt cttcgatgta atcgatcaat
    20161 tggttcagat ctttaagcat atttatcttc ctccttgctt gcctttttat catattatca
    20221 aagaaagtca attacttgat gaatcaggaa ccaatttagt cagatgtaga aaatgttgta
    20281 tggatctaaa gaaaattctc aagcagcgtt tcgtaacgga taattggtat tatctgccta
    20341 tctatgtttt acctttagaa aaataaattt tggggaagac acattctaag taaacaaagc
    20401 ggtaaaaata tcttttattc ccttagttaa tccgcttact acctactgta caaagcaaat
    20461 tgttgttcaa actgctgatc agaacgctgc ttggattttt ctttgcgata catcatacct
    20521 cttttatcaa ttggaacaat tatttatttc taacaaaatc acttcatttt tgtcagataa
    20581 ataaaa
//
LOCUS       DAZULO010000046        20485 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000046 DAZULO010000000
VERSION     DAZULO010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.047"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="ACG8OA_001623"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="ACG8OA_001623"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFS4976400.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="ACG8OA_001624"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="ACG8OA_001624"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFS4976401.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="ACG8OA_001625"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="ACG8OA_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFS4976402.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="ACG8OA_001626"
     CDS             2857..3732
                     /locus_tag="ACG8OA_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFS4976403.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFVLRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="ACG8OA_001627"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="ACG8OA_001627"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFS4976404.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="ACG8OA_001628"
     CDS             4629..5063
                     /locus_tag="ACG8OA_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFS4976405.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="ACG8OA_001629"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="ACG8OA_001629"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFS4976406.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="ACG8OA_001630"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="ACG8OA_001630"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFS4976407.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="ACG8OA_001631"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="ACG8OA_001631"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFS4976408.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="ACG8OA_001632"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="ACG8OA_001632"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFS4976409.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="ACG8OA_001633"
     CDS             13334..14260
                     /locus_tag="ACG8OA_001633"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFS4976410.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="ACG8OA_001634"
     CDS             14442..15335
                     /locus_tag="ACG8OA_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFS4976411.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="ACG8OA_001635"
     CDS             15469..15594
                     /locus_tag="ACG8OA_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFS4976412.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="ACG8OA_001636"
     CDS             complement(15728..16360)
                     /locus_tag="ACG8OA_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976413.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16508..17050)
                     /locus_tag="ACG8OA_001637"
     CDS             complement(16508..17050)
                     /locus_tag="ACG8OA_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFS4976414.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17185..17574
                     /locus_tag="ACG8OA_001638"
     CDS             17185..17574
                     /locus_tag="ACG8OA_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976415.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17578..18342
                     /locus_tag="ACG8OA_001639"
     CDS             17578..18342
                     /locus_tag="ACG8OA_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4976416.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18471..19418
                     /locus_tag="ACG8OA_001640"
     CDS             18471..19418
                     /locus_tag="ACG8OA_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4976417.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19548..20288
                     /locus_tag="ACG8OA_001641"
     CDS             19548..20288
                     /locus_tag="ACG8OA_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976418.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7192 a         3481 c         4317 g         5495 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcgtctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatctcca aattaaattg
    16381 ataagtatat aaagtataat aacaaattga tgagtatatg tcgatgtttt ttaatcaaaa
    16441 taataaataa aaactgtcta acatttaaaa tttaatttag atattagaca gtttttaagt
    16501 ttgtgagtta ttttccttgt cgttttctca cttttttagt ccagaaacca cctgaaatat
    16561 attttggctc ataagaaatg ataaaagcac gctcttcgat ttctttgatc aggcgataca
    16621 gttcacgttc gctttttcta ggagaaagta tttcgagaac tagccgctca ccttccagcc
    16681 ccattgcaga agtctgtgtc acaccataac cgtgttctct cagtataccc ggcaaacttt
    16741 tatcttctgc gctatttgct ggcaaaatcg ttgtgaccat gatgtatcct aatgccaatt
    16801 tgtcttcaat caaaattccg acactgatac ctactgcgta acctaacgca tagacaaata
    16861 aattcagtgg attatctaaa cgatctaaaa caagggataa gccgacgata taaatcgtga
    16921 tctcgaccat actgacaagt ggagcaatca gtcgatagcc tttcattgtc aacatgaaac
    16981 gtaacgtgtt cagagtgata taagcaaaat taatgaggaa gatcatcccc aacatttttg
    17041 gatcaatcat ttgtaaaact cctttcattt atataacaac aatatcaaaa aaaatcgtaa
    17101 gtgaagaaaa attgaaactt tatacataca aaaaaatgat aaaatgatta taatagaatt
    17161 ataaagatag gttaggtgat taaaatgaaa acggtcaatc gtgtgttttg tgccagccac
    17221 gtgctaagtc tccttctcag tatatactta ggtgtctatt cgattcaaca gccaattgag
    17281 gtagagaaca ttgtttttca gatgtcgctg acagatggtt taattttaag cgtttttttc
    17341 tttttgatac tgtttattgg taatattttc ggtagcgttg ccagtttttt gaatttttct
    17401 atttatccac tcctttcgct agcattagga gtggtgggac tttgttctct gtgcttattt
    17461 cccgagccat tcagtcctgc atttatatta tttggtacac tgaacctgtt ccagacaact
    17521 gttggagcat ggttgctatg gcgatcagga aatttaatga aaataggtga gtaacaattg
    17581 aatccaatta tattgaaacg ttctgtccat catattccag tgttagaagt tgttctgaaa
    17641 gaattaagag aggaacgtat tcccgttgtc atctattatc acggatggca gacaagtaag
    17701 gagttagttt tgacgcaagg aagaaaaatt gcgcagcagg gcattcgtgt gatcctgcca
    17761 gatgcgatga atcatgggga gcgcaaacag ccggtttctt ctattccatc ctatacattt
    17821 tgggatagta tctacggaaa tctgtttgaa tttgacacac ttatcaagca tttagaaaaa
    17881 agaggtcttc tacatgatca gcttgcagtt ggcggggttt cgatgggggg aatgacaaca
    17941 tgcgccttgt tggctaaaca tcctcatata gtagcgggcg tgtgtttgat gggttcccct
    18001 gcacctttgg aatatggaaa agaaatcgta aggcgcgcat cggaatataa ttatgtcgta
    18061 ccaaaagact attttgattt gatttcatgg gttaagagct atgatttgtc attgcagccg
    18121 gaaaagttag ctggacgtcc tctgttcttt tggcatggac aagctgacga aaaaatccct
    18181 tatatacaaa cggaacgatt tgtagaaaaa aatcggcata agtcttacgg aaaagaaatt
    18241 gtttttcgtt cctcaaaaac acagcgacat atggtacaaa taccactaat ggaagaagct
    18301 tccacttttt tagcaaaaaa tttgataaaa aagtataaat aattgaaacg gaaaatcact
    18361 catgacgata gaggattttc cgttttttat tttgggctat aataaactct gttaggaaaa
    18421 tttggtataa ttgatagtaa gaatgacaac atgttagaaa ggaagagagc atgcccactt
    18481 tagcagatgt tgcaaaacgt gccaatgttt caaaaatgac ggtctctcga gtcatcaatc
    18541 atccggaaca ggtgactgac gaattgaaag agttagtttt ctcagcaatg gctgaactgg
    18601 attaccgtcc aaatattgct gcaaaagcac ttgtcacgaa tcggtcgcag atcatcaaat
    18661 tatttatact ggaagaaatc gacacaactg agccctatta catgaatttg ctgatgggaa
    18721 tcgctaaacg gatcggtaaa gcgcattatt ctctccaatt agttacaaat gatgccttcg
    18781 acgtcggttc ttgtgatgga tacatcatca caggtgtcag aaaaaaagac ttcgattgga
    18841 tcgaccgttt ggaaaaacca gttgtcttgt ttggggaaaa ttctgaagga tatgattatg
    18901 tagacagcga taatgaatat ggtacagcaa aagcaacgca atatgcctta gaaagagatt
    18961 atcaaacgat tatttatatc ggtatcgaag tggacgagcc ttttgaaatt tcaagggaaa
    19021 ctggttattt atctgtgatg aaggaagttg atcttcctag taaaatcttg cggtttccaa
    19081 atcattcgac agaagtagaa agatatatga cagaaaactg ggcagatttt acagataaaa
    19141 cttgttttgt atgtagttca gatcggctag cactaggagc agtaagaggg attataaatg
    19201 ctggaggaca cttgcccgaa gaatttggtg tgattggatt tgatggggta tttttagacc
    19261 aaatcagttc acctaaattg acaacgatca agcaagatat tatacgtatg ggtgaagtat
    19321 gtggagaaat gttgttaaaa aaaatagaag aaaaaggggc gagtcaaggt tatcgtcgtt
    19381 tttttcctga actagttatc agagagacaa cgaattaagt cttattacta tatatatatt
    19441 tatgaaattc atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat
    19501 ctgtgctatt attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca
    19561 gacgaaaaca tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg
    19621 acaagaagcg agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc
    19681 cgtttgaaca gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca
    19741 agcgtcgtgc aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata
    19801 aaggaaaatt accggattat accggccatt accaatcaga aatcacatcg acaggacgaa
    19861 aaaaactttt tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca
    19921 agcatactac acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc
    19981 ctaaatatgt acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc
    20041 atttaatcga atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga
    20101 cgccgtatca agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa
    20161 cgttagagga aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag
    20221 aacgctcatt acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt
    20281 ttaaataagt gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt
    20341 ttccttattt tatactctat ccgaaagttt atatcgtatc ctcaaatttt tttgagttta
    20401 agcgaatttt ttctcccaat aagtaagaaa ataatggcaa aataaaaaaa caaactattt
    20461 tcgcagatcg tttgcaaaaa tagtt
//
LOCUS       DAZULO010000047        19969 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000047 DAZULO010000000
VERSION     DAZULO010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.048"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..598)
                     /locus_tag="ACG8OA_001642"
     CDS             complement(<1..598)
                     /locus_tag="ACG8OA_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFS4976419.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKL"
     gene            871..3342
                     /locus_tag="ACG8OA_001643"
     CDS             871..3342
                     /locus_tag="ACG8OA_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4976420.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3445..5181)
                     /locus_tag="ACG8OA_001644"
     CDS             complement(3445..5181)
                     /locus_tag="ACG8OA_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4976421.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5178..5882)
                     /locus_tag="ACG8OA_001645"
     CDS             complement(5178..5882)
                     /locus_tag="ACG8OA_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4976422.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6013..6516
                     /locus_tag="ACG8OA_001646"
     CDS             6013..6516
                     /locus_tag="ACG8OA_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFS4976423.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6476..6814
                     /locus_tag="ACG8OA_001647"
     CDS             6476..6814
                     /locus_tag="ACG8OA_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFS4976424.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6835..8253
                     /gene="ffh"
                     /locus_tag="ACG8OA_001648"
     CDS             6835..8253
                     /gene="ffh"
                     /locus_tag="ACG8OA_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFS4976425.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8365..8943
                     /locus_tag="ACG8OA_001649"
     CDS             8365..8943
                     /locus_tag="ACG8OA_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFS4976426.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8947..9468
                     /locus_tag="ACG8OA_001650"
     CDS             8947..9468
                     /locus_tag="ACG8OA_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFS4976427.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9547..10101)
                     /locus_tag="ACG8OA_001651"
     CDS             complement(9547..10101)
                     /locus_tag="ACG8OA_001651"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFS4976428.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10354..10629
                     /gene="rpsP"
                     /locus_tag="ACG8OA_001652"
     CDS             10354..10629
                     /gene="rpsP"
                     /locus_tag="ACG8OA_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFS4976429.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10641..10892
                     /locus_tag="ACG8OA_001653"
     CDS             10641..10892
                     /locus_tag="ACG8OA_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFS4976430.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11017..11808
                     /locus_tag="ACG8OA_001654"
     CDS             11017..11808
                     /locus_tag="ACG8OA_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976431.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11978..12499
                     /gene="rimM"
                     /locus_tag="ACG8OA_001655"
     CDS             11978..12499
                     /gene="rimM"
                     /locus_tag="ACG8OA_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFS4976432.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12489..13250
                     /gene="trmD"
                     /locus_tag="ACG8OA_001656"
     CDS             12489..13250
                     /gene="trmD"
                     /locus_tag="ACG8OA_001656"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFS4976433.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13386..13733
                     /gene="rplS"
                     /locus_tag="ACG8OA_001657"
     CDS             13386..13733
                     /gene="rplS"
                     /locus_tag="ACG8OA_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFS4976434.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13865..15001)
                     /locus_tag="ACG8OA_001658"
     CDS             complement(13865..15001)
                     /locus_tag="ACG8OA_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4976435.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(15117..16031)
                     /locus_tag="ACG8OA_001659"
     CDS             complement(15117..16031)
                     /locus_tag="ACG8OA_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFS4976436.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(16122..16550)
                     /locus_tag="ACG8OA_001660"
     CDS             complement(16122..16550)
                     /locus_tag="ACG8OA_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS4976437.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(16555..16929)
                     /locus_tag="ACG8OA_001661"
     CDS             complement(16555..16929)
                     /locus_tag="ACG8OA_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4976438.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            17215..17400
                     /locus_tag="ACG8OA_001662"
     CDS             17215..17400
                     /locus_tag="ACG8OA_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4976439.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            17412..17660
                     /locus_tag="ACG8OA_001663"
     CDS             17412..17660
                     /locus_tag="ACG8OA_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976440.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            17691..17870
                     /locus_tag="ACG8OA_001664"
     CDS             17691..17870
                     /locus_tag="ACG8OA_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976441.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(17913..18383)
                     /locus_tag="ACG8OA_001665"
     CDS             complement(17913..18383)
                     /locus_tag="ACG8OA_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976442.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            18470..19168
                     /locus_tag="ACG8OA_001666"
     CDS             18470..19168
                     /locus_tag="ACG8OA_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFS4976443.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            19346..19687
                     /locus_tag="ACG8OA_001667"
     CDS             19346..19687
                     /locus_tag="ACG8OA_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976444.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            19680..>19969
                     /locus_tag="ACG8OA_001668"
     CDS             19680..>19969
                     /locus_tag="ACG8OA_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS4976445.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         6552 a         3506 c         3923 g         5988 t
ORIGIN      
        1 ttagtttgta aagtctgcga tcttcaagga tcgtaaatag ctgattggtt gtcccatcaa
       61 cacaaataaa actcatggaa ccagaaacag aattcattga tttgaactca tccatacaca
      121 acaccgtcgg aaggaagttc atacgtggct ggtaatgctt cgttgcttca cgcaagatcc
      181 gtgtcaccgt cccatccgag acgaagtact tcttagcgat tagtttacgt gattgatttt
      241 cgtacagttc catgagaatc acctgcttca gttcctttgt tagttgatga tgttcatcaa
      301 ctaagtccgt tttcgcagaa aaggtccttc cacaatcatg gcagagaaaa cgggtacgtt
      361 ttaattctaa aatagttaac ttttctttga ctttcaacat ctgagtgcgt gtcgtatagg
      421 aaccgttgcg gataatacga accgatcgac agtgttcaca ataggaagaa gagtcatcca
      481 attttccttt aataaactgc gcttgaacgc ctttatattc tttttcaatc agccattctt
      541 catcaaaaaa taaatgttta tctgttaatc cgagtaattt tctagtatga ttatccattg
      601 agatactcct ttggtaggat gacgttttgg tcgactaaat tttaccaaaa gtgagtctct
      661 ttttgagttg aaacgaagaa aaagtgttag tagaaaatcg ccttcgattt tccaccaaca
      721 caaaatatta tagaaccctt tttttttgct atatgacagg aatgaaaggt gtttgcaagc
      781 ttaatgtgag gaacactttc catatctgtt ctatactgga taggagaagt ttggaaatat
      841 ccagaaaacg aaatcaatcg aggtagccac atgaagaata aatggataaa tcgaagtaaa
      901 aaaaagaaag taacacaaag atcatcagat aaagtcatcc gtctttttcc atcgtataaa
      961 gaaggattaa cggcagatca agtcaaggaa cgaatagaaa aaggtgcagc aaataattca
     1021 gtcgatccca cattcaaaac aaatcagcaa atcgtattgg aaaacatatt tacgtatttc
     1081 aatttgatct ttcttatctt agccattttg ctttgtttag tagaatccta taagaacttg
     1141 acttttttac cagttatcat agccaataca ggaatcgcca tttttcagga aattcgatcc
     1201 aaaaagatct tggacgaact aaatgtttta aatacagcaa aagtcaatgt catacgagac
     1261 gggatagaac aaacaataga tatagaagac cttgtaatag atgacattgt tattttagag
     1321 actggatatc aaattccagc agatgcagag gtcgtggaag ggaaactgca agttaatgag
     1381 gctttattga caggcgaagc agatgaaata accaaagaaa taggtgatcc attaatgtcc
     1441 ggcagtttca ttgtttccgg aaaagcttac gcccgattag acaaagttgg agcagattcg
     1501 tatatttccc agctgacagt caaagccaag acaatgggag aaggagaaca gtcggagatg
     1561 attgcttctc ttaatcaatt gatcaaatgg attgggatca tcattattcc tatcggtatc
     1621 ctgctttttt ctcaaagtta cttttttaat ggaaacacga tcaaagaaag tgtcgttgca
     1681 atggaagcag cattgatcgg tatgatacct gaaggactgt acttgctgac gacgattgca
     1741 ttagcattaa gtgcgactaa actagcaaag caaagagttc ttttacacaa tatgaaaagc
     1801 attgagacat tggcaagggt aaatgtcctt tgtgtagata aaacaggaac gatcacagaa
     1861 aataaaatgt ctgttcaaaa aatgatcgtc tctaaaaaac aagaaataat cagtcagtca
     1921 gatcaattgg aagcatgcat tgctgactat gcaaaagcga tggcaggaga caatgcaaca
     1981 atggaagctg ttaaagcata ttttacaaaa accactgatc aatcctatca ccatgttatt
     2041 cctttttctt ctgtccaaaa attcagcagt gtagcgttgg cagacaaggt gtatgtttta
     2101 ggagcgcccg aaatggtttt gcgcgatcaa ttgccggaat atgcggaaga gtttgtttct
     2161 ttcgctgaaa aaggctatcg cgtgttggtc tttggtgtgt acgatgggat tcttgaagaa
     2221 ccagtattga cagaagcagt cacaccatta ggctatattc tgattgctaa tcctatcaga
     2281 aaagaagcaa gagccacatt tgattacttc aaaaaacagc aagtagccat caaagtgatt
     2341 tctggagata atccgctcac cgtatccaat gtagctgtcc aagcagggat ttcacatgct
     2401 gatcagtacg ttgatgcatc taaattatca gaagaagaat atgaagcagc tgtcgaaaaa
     2461 tatacagttt ttggacgcgt caagccagaa caaaagaaaa tcttcgttca attgataaag
     2521 cgtaacaata cagtagctat gacaggtgat ggcgtgaatg atattctagc catgaaagaa
     2581 gcagactgca gtatagcgat ggcttccgga aatgaagccg ccatgcaggc ctcacaagtg
     2641 gtcttacttg attctgactt ttcacgcatg ccagaagttg ttgcagaagg acggcgcgtt
     2701 gtgaacaata ttgagcgatc agccagcttg tttttagtga agaatatttt ctcctttttg
     2761 ctctcactgt tttcagtgat ctttgctctg acctatcctt tagaaccatc acaaatcaca
     2821 ttgattagtt tgtttactat cggacttcca tctttcttgc ttgctctaga agaaaacaaa
     2881 aagcgaatca gagggaaatt catcatgaat gtgatggaaa aagctgtgcc aggtggacta
     2941 acagatatga tcgtagtcgg tgcgttggtg atctgcgggg tgactttgaa tctaaataaa
     3001 acagatgttt ctacagcatc taccatgctt ttgattgcag tagggttcct cgttctctat
     3061 aaaatctgtt caccgctgaa taaattcaga agtcagatca ttcttttctg tgctagcggt
     3121 aacttttttt cggtcatctt tttgcataag ctgttttcaa tcaccggtat ctcagctgta
     3181 tcacttttgc tgttgagcat tctatttttc tcggcagatt cgatttttag gcatttgacc
     3241 acattggtag aatatttgtt tgaacgtaaa aatgaaaaag agcaaaagcc gtttagtcgc
     3301 gtagctaaaa aatttctatc gatatttaaa cgagaaaatt gacgaaaagt atctcaaaaa
     3361 agagtaaaaa agaacaacca ctcgtgtatc tttcgaagca ggatgagtca tccaacttta
     3421 aaacgcacga gcagttgctc ttttttacat tttcggaatg gtaacggtga aagtcgaacc
     3481 tgttccaaga tgactgtcta cagtaatcgt accattaagg agttgcacgt agtcttttac
     3541 aatcgctaac cctaatccgg tcccgcctga atgtcggctt cgcgccttgt ctacgcggta
     3601 aaaacgctca aagatccgtt cctgatcctc aatgtcgata cctataccaa agtcttggac
     3661 agaaaacgat agatcatctg ttgcctgata gcggataata atctcgctgt cgctttttga
     3721 atattggaca gcattttcaa ttagattttt gataattgga taaaggatat ctgttttagt
     3781 agtaaatggt tcgttctttc caatcaatcg aatcgtcaac cgtttctttt cgatgattgg
     3841 ctgataattt tgaacgattt gctggaaata aggttctgta taaattgtct ggatctcgta
     3901 agaattctcg ccatcttttg acagctggat gatttcccga atcagtttat ctaaccggat
     3961 cgcatctttt tgcatgatct caagaaaaga agtcagagtt tgcgggtcat cttttgcacc
     4021 atccagtaaa gtctcggtaa aaccaatcag tgaagtcaca ggagttttta attcgtgaga
     4081 gacgttgcca acgaaatcct tttgtagctt ttctaaacgt cgcactttcg ttaagtcata
     4141 cgccacccct agaatctgtc cggtaccttc tgaattagta aagtagcgca aactcaagtc
     4201 taatgtctgc cgaccttctg taagggtgat ctcctgatgg atcagcggat gctcaggagt
     4261 cacttgatga atcagctgaa tgagttttgg ctcttgaatg acttctgtgt accgctgttc
     4321 aggttgatag cttaccacgt ttaaatgtag ctgcatcttt ggattaagca gtagtaaacg
     4381 agaatcagca tcatcaatca ggaatacgcc aatcatcagt tcgttcagta acgtatataa
     4441 ttgctcttct gtggtagtac acgcccgata catcttactc atttgttcgc tcaacgcatt
     4501 aatcgtctga tacaattctt cccattgatc gggtgtttgc atgattactt ccgcctgatt
     4561 aggattttgc accatttttt ttagtacggg cagcacggtt ttcaatggct ggtttttttg
     4621 atagatcaga taatagatca ttcctgcaag aacaaggaag aaagacagaa agaagaaaaa
     4681 gacccaacgg cgaaaactct cagttctcgg taaaaaaccg cttgtcggtt ctgcgatacg
     4741 taaaatggct tctaactggc cacttttttt gactggtaat gcgacatata aaagatcctc
     4801 attcaaagta gtacttttgc gcaaagcgct acctaggttc ccaccattca atacggcttt
     4861 gatttcggga cgggagtttc gctgttcatt caatgtttca ttgtttgtgt caaataagat
     4921 ctttccagta gcatccatta aagtaatccg ttcgttcgac tgatggacga attcttccaa
     4981 agcagttttg ttttgcgctg cttggaggtt ttcgacatct aattgccgga tcagcagttg
     5041 tgcttttttt gtcaggtagc tttcttgctg ttccacgatc tgctgttgaa aataatggga
     5101 aatcaattgc cagccaccaa aaaacaaact aactaaaaga ataagaaaga agagacgttc
     5161 ataaattttt ttccatttca tcgtttgggt tcctgaaact tatagccaaa tcctctaata
     5221 gtcaccaagt actttgggtg ttttggatct cgctcgattt tttctcgcaa atgactaacg
     5281 tgtacatcta cgattcggct ttgtcctgcg aagtcaaaat tccaaatgcg gtctagcaat
     5341 gtgtctcggt cgatcacacg gtcttttcgt ttcatgaaat aaacaagtaa ttcaaattct
     5401 ttcggtgtta gttcaatcag atggtcgtct acggtcactt gataattggt caaatcagca
     5461 gtgatttgtc caatcgatag atattcaggc tcagcttcat cttttgattg cttacgaggt
     5521 tcaattcgtc tgaatattgc tttcatacgg gctaatactt cgcgaggact aaatggtttg
     5581 gtcaaatagt cgtcagcacc tatttctagg ccaatgatcc gatccacttg gtcatcttta
     5641 gccgtcagca tcaaaatcgg tgtttcattt ttttcttgac gcagccgctg agtgatctcc
     5701 atgccatcca tcgttggaag catcacatct aaaatgatga aatcaaatga ttgttctaat
     5761 gctaattcta atccttcgcc tccatctgtg gcactggtta ccttgtagcc ttctttttcc
     5821 aaattgaacg tcaagagcgt tacaatagaa ggttcatcat caacaactag tatttttttc
     5881 attcaaatct cctttaaata ggtttcattc tgtttatatt ttagcataga atcagtacaa
     5941 agggctattt tacaaaagaa ataaatggta taataggaag gcactttgct gaatagaaag
     6001 gaagagcagt caatgattgg aatcagtgca tgtttaggcg gtctggcctg tcgctatgat
     6061 ggacagaaaa aagaagtacc agaattgatg gaactagttg aaactggaga agctgtcatg
     6121 gtctgtccag aagtgatcgg cggattacca attcctagag atcccgctga aatcattggt
     6181 ggaaatggat tcgatgtgtg gaaagatcaa gcaaaagtat ggacgacaag cggcgaagat
     6241 gtaacggaag cgtacaaaaa tggcgcgatc aaagcctatc aaaaattgca ggaaaaacac
     6301 attgatctag tcatcatgaa ggaaaaaagc ccttcatgcg gaacccattc gatttatgac
     6361 ggaagttttt ccggaataaa aaaaaatggt gccggagtgg ctactgctta ttttattcag
     6421 caaaaaatga ctgtgctctc tgatgaagaa tggctgaaaa ctagaggtga gcagaatgga
     6481 aatcgaaaag accaatcgaa tgaacgcgct ctttgaattt tattcgacct tattgacaga
     6541 aaaacaaatg aactacatgg aattatatta tgcagatgat ttttcattag gtgagatcgc
     6601 ggaagaatac gaagtaagcc gacaagcagt atatgacaat atcaagcgga caagcaagat
     6661 cttggaggat tatgaaaaaa aactccatct tttttctaat tatattgttc gagaacaggt
     6721 attggaaaat atgactgcat atattacaga taagtacccg gaagataaaa aactacttga
     6781 atatgtacag caaattcaag caatcgaaga ataaataaaa ggaatgaaga aaatatggca
     6841 tttgaaagct taactgaacg tctccagcag gcgatgggaa aaataagaaa aaaaggaaaa
     6901 gtatcagaag ctgacgtaaa agaaatgatg cgggaaatcc gccttgcctt actagaagcc
     6961 gatgttaact tgcaagtagt caaagatttt actaagcgcg tacgcgaacg tgcggtaggg
     7021 gcagaagtgc tggaaagttt atcacctgca caacaaatcg taaaaatcgt tgatgaagaa
     7081 ttgacgatca cacttggttc tgaaacagca gagttaaata aatcaccaaa aataccaact
     7141 gttatcatga tggctggtct tcaaggggca ggtaaaacga cctttactgg taaattagcc
     7201 aattatttaa agaaaaatga aaatgctcgt ccgctcctaa tcgcagggga cgtatatcga
     7261 ccagctgcga tcgaccaatt gaaagtatta ggtcaacagc tagacgttcc tgtatttgat
     7321 atgggaactg aagtcagccc ggtagagatc gtccgtcaag ggatggagtt agcaaaagaa
     7381 aagaaaaacg actatgtatt gatcgatacg gcaggacgtc tgcatatcga tgaaacattg
     7441 atggatgagt tgaaacaaat caaagaattg acgcaaccaa atgagatttt gcttgttgtc
     7501 gatgccatga ctggtcaaga tgccgtcaac gtagctgata gcttcaacca gcaattaggt
     7561 attacagggg tcgtcatcac taaacttgat ggggatactc gaggcggggc agcactatcg
     7621 atccgctcag tgacaggcgc accaatcaaa ttcatcggtt ctggtgaaaa attgactgac
     7681 ttagaagtat tccacccaga tcgtatggct agccgtatcc ttggtatggg ggatatgctg
     7741 acattgattg aaaaagcgca gcaagactac gacgagaaaa aagcggaaga acttgcacag
     7801 aaaatgcggg aaaattcatt cgatttcaat gactttattg aacagcttga tcaagtgatg
     7861 ggtatgggcc cactggaaga tctaatcaaa atgatcccag gaatgaatca agtccctgga
     7921 atcgaaaacg tcaaggtaga tccaaaagat gttgaacgca aaaaagcgat ggtttattct
     7981 atgacgccag cagaaagagc aaatcctgat ctgttgaacc caagccgtcg tcgccgaatt
     8041 gctgccggtt ctggaaacag tgttgttgaa gtgaatcgaa tgatcaaaca attcaaggaa
     8101 tcaagaaaaa tgatgcagca aatgtccaaa ggggatatga acatcccagg tatggaccaa
     8161 atgtttggtt caggcgtcaa aggaaaacta ggcaaaatgg cgatgaatcg aatgatcaag
     8221 aaaaataaga agaaaaagaa aaaacggaaa taagatgaaa aaaacagctt cggtacagac
     8281 tgaaaatgtc tactgccgga gcttttctat cagcaatttt tattctttac taatacaatt
     8341 actctaaacg aagaggtggc acagatgaga tgtccgtgga gtgaacaaac agaaagtatg
     8401 cggcaatatc atgatactgt ctggggtgtt cctgaatatg atgaccaaaa gctgttccga
     8461 aaattgatgc ttgatattaa tcaagccgga ttgagctggc agacgatttt aaataaaagt
     8521 gaagcattcg atgaagcata tgatcgattc gatatcaaaa aagtcgcaaa ttatggagaa
     8581 acaaaaatca gtgaactaat ggaaaacaaa ggaattattc gtaaccgaag aaaaatcgaa
     8641 gcagccgtcc aaaacgccaa agcagtcttg aaaattcaag aagaaatcgg cagtttttca
     8701 gagtatcttt ggtcgttttc taatggaaaa atcgttgatt cttcttatca ttcacaagaa
     8761 gaagtcccaa caacaagtcc tctttcagat gaattagcaa aagatctaaa gaaaagagga
     8821 tttaaattta tcggaagcac aacaatctat gcttttttag aagctgtcgg gatcatcaat
     8881 gatcaccttg acagttgttt ccgcaaagaa gaggtcgtac gtatcggcag aaagggcgaa
     8941 taaagcatgc gaaaaatcat attttatggt gctataagtt tagatggcta tttggcaacg
     9001 aaaacggatg atcttcaatg gctttttgac actccaacag gtaagcaaac tacttatgaa
     9061 gctttttatg aaacaatcga tacgaccatc atgggcagaa aaacttatca ggaagcaaaa
     9121 aaatatttgg agaccgaagc aatttatcca gagaaaaaga atttcgtttt ttccacccac
     9181 gcacaattac aattgccaga tgctgaggtc gtaaacgaag atcctgttgc ttttctaaaa
     9241 aatctacaaa tgacagaagg aaaagatatc tgggttgtgg gtggcggtcg attgatcaaa
     9301 ccgctattgg aacatcaaat gattgatgaa tggtatatcc agattacccc tgtattatta
     9361 ggggacggga ttcctttatt ccaaaaagga acatatgaag cgcgttttga aatgctagac
     9421 acgacacgat tcggtgaatt tatcgaattg cattatgtac gaaagtagaa gtaaataaaa
     9481 taaaaaaggc aactatatgc agaaatgtcc tgtacatagt tgcctttttt attaccgtta
     9541 tgctttttac tctgttaatt tgttagaaaa atctagatac gcatctaaaa tcgtttggaa
     9601 gaaacttaat gttccttcaa cgatattacc attttcatca attagattcg caacgtttcc
     9661 gatatatgct tccggttgct gcaaggttgg cacattcaaa aagacaagtg attggcgtag
     9721 atggtggttt gcgccaaagc cgccgatccc acctggtgat gcagtaacga ctaaacctgg
     9781 tttatgatcc caaacactgt gtccataagg tcttgagcct acatctaaag catttttcaa
     9841 tactgctggc acagaacggt tgtattcagg agtaacgaaa acgacaccgt ctagtcccct
     9901 cacttcctca cgaaaacgag tccattcagc aggagcctgt tctggagttt cgagatcctc
     9961 attgtaaaat ggcagatcat cgatttttac aaatacactt tcgtaccctt caggaagcaa
    10021 tttcgcaaaa gcttctgcta cttttttgtt gtaagaatct tttcttaaac taccaataaa
    10081 aaatccaatt ttttttgtca tacgattttt cctccttcaa actatctata ataatcttac
    10141 aaaagataag taactggagc aaataaaaac acttactgac aaaagctaca tagaagatag
    10201 attacttatt tttaacaaaa aagaagtcta aaatcttcta ttttctaaaa aaaatcgaat
    10261 ttaatcaagc tgtaaagaaa aaaagcttta cagggtaaaa aaaaactgtt acactacatc
    10321 ttgtgaataa aacaatggag gtggaaataa ataatggcag ttaaaatccg tttaaaacgt
    10381 atgggttcta aaaagagtcc gttttaccgt atcgtcgtag ctgattcacg ttctcctcgt
    10441 gatggacgtt tcatcgaaac tgtaggtact tacaatcctt tgaaagaccc tgcagaagta
    10501 gttttaaaag aagacttagt tcttgactgg ttatcaaaag gtgcacaacc ttctgacact
    10561 gtacgtaaca tcctttcaaa agaaggcgtt atgcaaaaac accacgaagc taaattctca
    10621 aagaaataag gtgaccggat atgaaagatt taagcgactt agtcttaaca atcgttcgtc
    10681 cgctagtcac ttatcctgat caagtcaagt tagacgtcgt agagtcgaaa gacttttacg
    10741 agtacaatct aacagtggct cctgaagata ttggccgtat cattggaaaa caagggcgtg
    10801 ttgctaaagc aatccgcaca atcgtttacg gtgttcggat caatgcaccg aaaaaagtgc
    10861 gtttgaatat catcgatgat aaggaaaagt aaataaacgt gctgtactcg aattttcgag
    10921 tgcagctttt ttgttttttt tattaattga agaaatgatt atttggcaaa acaagcaaga
    10981 aagcgtatcc ttataggagg agggagtgaa cagccaatga gaaagaaaat gttatttatt
    11041 ccatttgctc tattgttaag cgcttgtagt tcggttactc aagaatcaga caatacacat
    11101 ctatcaagca gtatatctga aactgttacc agtccagaaa aaagtagtac caaaacggct
    11161 tccaccactg aacaaacaat ggctacctca aataatgaga aaaagactgc tttagaccag
    11221 ttgaaagaac aacagccaaa tgttcccatg cctctagatg taccagtctc ttcaggctat
    11281 ctcaatatcg cagcgacaca tacaaagcaa ggttattcta ttttatatta cagaacagat
    11341 cgtcccctcg ggttgaatgc tgacgaattg aatcaagaaa cgcctattgc aacctatcta
    11401 tatcaatatg ggtttgcttc tagtcaagaa acaatccaag ttttgcagcc atttgagatc
    11461 gacacaaatg gtcagcaagt ggacttaggt tctcgaatca cagggtatca gcaaggtgca
    11521 gcaggttcaa gttttcttga atggcaagaa ggaaactggc gtattcgaat ccgtgggaat
    11581 aatatagaag gccaagatcc cttactatta gctaaagaaa tcgtagctta tttggaagaa
    11641 aacagtctac cagcacctga acaatttggt aaaattactg ttgatatggg tgatacgacg
    11701 aatcgagccg tagaagtaag ctggcaagaa ccgaaaaatg cctatacgat cactcatcaa
    11761 gatcctatgt ctgcgttgaa gatggcagtt tctatgaaga ggttgtgaaa tcagcgcgct
    11821 gatctgagcc catcatttaa ctccaagtca caggaagtaa tcaaaaatag cacgccgcat
    11881 ttttgcataa ttttggctta ataccttaaa agaaaactag aattgcctct tcacacatgc
    11941 taaaataaaa cagacaaaaa agaaatgggg atataagttg actgaatact tgaatgtagg
    12001 aaaaatcgta aacacacaag gtatcaaagg cgaagtccgt gttatctcaa caacggattt
    12061 cccagaagaa cgttacaaaa aaggaacggt gctaacacta ttccaagacg gaaaagcacc
    12121 tgtggaatta acagtcaaaa gtcatcgcaa acataaaaat tttgacttat tgagttttga
    12181 aggacatcct tctatcaatg atgtggaaaa atatcgggat ggcatattaa aagtgtcaaa
    12241 agacaactct gtagatctag ctgaaaatga attctattac catgagatca tcggtctgca
    12301 tgtattagaa gatggaactg agttaggtaa agtaaaagaa atcttgtcac caggtgccaa
    12361 tgatgtttgg gtcgttcaac gtccgaaaaa agctgatata ttgcttccat atatcgcttc
    12421 tgtcgtcaaa gaagtcgatt tagaagctgg agtggtccgt gtagaaattc cagaaggact
    12481 gattgacgat gaagattgat gtattgactt tatttccaag aatgtttgaa ggtcctatgg
    12541 gggaatccat catcggtaag gctgtagata aaggattatt agatatcaat atctctaact
    12601 tccgcgatta ttcagataac aaacatcaga cagtagatga ttatccatac ggcggtggag
    12661 caggtatgct gctcaaagtc cagcccatct atgataacat ccaagccatt gaaaaagctg
    12721 caccggatgt caaaaaaaga gtaattcttc ttgacccagc tggtaaacga ttcgatcaga
    12781 aaatggcaga agaattttca caagaagaac acttagtatt catttgtggt cattatgagg
    12841 gatatgatga acgtatccgc acattagtga cagatgaagt ttccttagga gattatgtcc
    12901 ttactggggg agaattaggt gcgatggtca tgatcgatgc aacagttcgg cttttgccag
    12961 atgtgttagg gaatcagact tcagctcaaa cggattctta ttcaaccggt ttattggaac
    13021 atccccaata tactcgcccg gcagaatata aagggatgaa agtaccagaa gtattgacaa
    13081 atggaaatca taaattgatt gaagaatggc aattaaaaga atcactaaga aggacctatc
    13141 aacgtcggcc tgatcttttg gaaacacttg aatggacacc tcaaatgacg aaatttttag
    13201 aagaaatcaa gaaagaagaa caagaaaaaa cagcagatga agccaagtga ctttttctaa
    13261 aatcttcttt acagtaggtt gataatatgt tagtatttta tggtgagtgc aaaagcactt
    13321 aactattacg atattccgct gtgagaaaag ctcataagaa tatttggaat aaggagaaga
    13381 aaaccatgaa tccattaatc gaagaattaa caaaagaaca attacgttct gacatcccag
    13441 cgttccgccc tggtgacact gtgcgtgttc acgcaaaagt tgttgaaggt actcgtgaac
    13501 gtatccagtt atttgaaggt gttgttatca aacgccgcgg tgctggaatc agcgaaactt
    13561 atacagtacg taaagtttct aacggagtgg gcgttgaacg tacattccca ttgcatacac
    13621 cacgtgttgc aaaaatcgaa gtagttcgct acggtaaagt acgtcgtgca aaattgtact
    13681 acctacgtgc attacacgga aaagctgctc gtatcaaaga aatccgtcgt taatcatcgt
    13741 tatcagactg agaaagaacc cttggtattc aagggttctt tttttgttct ctgttttttt
    13801 atattaacga tttctatttg ggacattttt gattaataaa aaggggcaaa aaaggggcaa
    13861 caagttacag tttttctata tcttctttca atttttcttt tactcccttt gtcacatgca
    13921 aataaatctt ttcagtgata tcactatttt cgtgcccaac gcgatcctga atagcataca
    13981 gaggagttcc tagttccgct aattttgaaa tatgggtatg tctaaaaata tgtgaactaa
    14041 gttttttatc aatcttcatg tcagctttat gatttctcaa ataggtgtta attgctgtta
    14101 gttggaaagg agttcctttc gttgtttgaa atagaaattg accattcgga tttaattcta
    14161 gaagctcatt gtaaatagct atcgcttttt ttggtaaatc aatttctcgc attccagcag
    14221 cagtttttgt agaatcagat tttttcatat cagctattga acgttctcta tacatcatcg
    14281 tcccatttat aactactgat gcattattat tggtaatgtg tacatcgtct ttacttaatg
    14341 cgattgcttc tccaggcctc attccagtca aatatagcca ctggaaaagg agagaatatc
    14401 ttttattgtg tgaagttgtg aaatttacta gtcttttata ttcatcatct tctaaaaact
    14461 tatctttaat ttttatcgtt tttgactctc gtttataatc aataactact tcgttgatag
    14521 gatttttttc tacatagcct tttttcatag cgtatgaaaa aagaagattc aatttggatt
    14581 taattacact gacgtactta tttgacaagt catctttata tatcatatct tcaaaaatat
    14641 tatttaaatc tactgtatta ataccagaaa cgatgtaagt ctcaggtatc tttttcttta
    14701 tagtgtttaa gatattattc gtaggataat atgtagactc cttaacttgt cttttgtaaa
    14761 taactaacca ttcttcgaca agctcatgaa atgttagatc aggcttctga agtgtttttt
    14821 cgtttaattt tatatcaatt tttttattta actctagcaa tgccactttc tgagtttctc
    14881 gagatttatt tttataagta atgctcactt tttttctttt tctagttttt ggatcaatat
    14941 atcgctcatt atatttatat actttttttc cgtttttatc agttttggtt tcaatccaca
    15001 ttgttttcat ctcctatttg ttgctataat aggaatggat aagtaagcca attatagcag
    15061 gtttattttt catcacgtcc acaaactttg gcgagggagg gggcgtgttt ttttgtttag
    15121 tagctacctt tccatgaagt atctgcgaag aacacttcaa atgttgttcc atcatttttc
    15181 acttcaaaat atgctgttcc ttgtgcggat ttacccggtt ggatggtttc cgaaaagtaa
    15241 tctctactag ctaaatcaga tttaatatct gatgaatcat aaaattctaa ataacttgaa
    15301 cttgcatcaa aaggtgtagt ccctgtgttt tctacaagaa aatctacctt tgcgtaaaat
    15361 aggccttctg gtttatagaa atcatcaccg taatcttttg atacagactg gacagttacg
    15421 ttaatagagt taccattttc agaagtaaaa ggaactgctt ctcctatact atgtgcaact
    15481 gatgccgtct ggggttctga agacgaagtt gtagttgtag ttgtagtaga gcttgaatct
    15541 ttggattttc catctttatc taagaaagtt cgttgattat aatatacaac ctgttcagtt
    15601 tttggactaa cccaaattaa cattgtttca tcatcagaat cagttgaata agtatattgg
    15661 agcatctcta atttttttat atcttttgca ctatttccat gttcaattaa ttcagagtgc
    15721 tctttataat aatctccttg tgcatccttt gaaatagata caattaattc atcccgtgtg
    15781 atagcattgt ctaattcttt gtaaacaaaa tcatttgtcc atttacgtaa cggtgaacca
    15841 agtgtctgat aaacttgagt taaatcatca ccaactttta ttttatgaac gtctgatgaa
    15901 gttagttttt ttgtttcata ttcagatgtt gatgaaacag tattttcttt agtgctagca
    15961 gaattaggac tagtggagca tccgcttaat accaagccta aaaaaagcaa cccaaaaaca
    16021 atttttttca tttttaattc ctcacttcta tgatatgatt tttttgtagg atcttagaaa
    16081 taaggttttt agtccgtgtt gcagcacgga ctttttttac attatataag aaagcgataa
    16141 gcgctttctg gaagtccgta aagattcttt aattcctcaa ttcgtttagg atattgatca
    16201 ttgtcttctt tgtatagaga aacaatgaga ttagcagcaa agcaattagc ttcgctttca
    16261 gatttgcttc tagatgttct tgttgataca tagtaactgg ataagccacg atgaaaaata
    16321 gcgtgaccta attcgtgagc gcaaatgtag aatctttcct cagagtctct cagttcatca
    16381 tttaagaaga ttattgcacg acctctaatt tcttgaaact gtcctttggg attctcgata
    16441 aaaggaacgt attgaatttt aatgcccatc ttttcacaaa tataaaaagg attagcggac
    16501 tggtatttcc gcttcaactc ctcgactaaa ttaatcgtat ccatctccat aagctcacat
    16561 ctttttgcct ttttctttgt cttctttcac aatatcccag aaggttccaa taaggatatc
    16621 ttttacgcgt tggacctgtt caggcgtcag tgtttctccg ccataagaca tattgacatt
    16681 tgaatctagt aatttatcaa gttcaactac ttcatctttt gttgcccagt caggaacttg
    16741 gtttctaccg agtaaataat cagtagttac attaaaataa tctgctacaa gttgtagtct
    16801 tttagtacta ggagttctat ttttccattg ataaatcgtg tttttaggta tatttaattc
    16861 ttcttccaat tgagttatac ttatgcctct tttatgagct aattccttta ttctatctag
    16921 taaattcatt ttcgcacctc acaagctacg aaaacactaa taaaaaaatt agttaaaggt
    16981 gttgacaact aataaaagtg ttagtatact gttttcgtaa gctaaaatat tagctaaaaa
    17041 gacttgataa ctaataaaca cttcacggtc ggcaaactct gaaatgtaaa ttactaggca
    17101 tttctgtgtc ttatttagct atgtctatat actaataaaa atattagtta ttgtcaacga
    17161 atattagcta attttttagc ttacagatta tttttttaga aaggagctat ttttatgtct
    17221 gagaatttag acttaaaaat tcgagcggag atgagaaaaa gaagaatgac tttcaaagaa
    17281 ctagctgcgc ttatcggtat ttcaggagct tatttatcag atattctaaa cggcaatcgt
    17341 gatggaaaga aagcacaaca gcatatcgaa acagtgaaaa aaatattgga catccgatag
    17401 gggggtaggc gatgacaaaa ctaaaaaaac aagattttgt aaaaaaatac aattattctc
    17461 catctactta tcaacgtcga atgtcggaac taaaaaatac agcaattttc tcagcggcgt
    17521 atgaacgggt cacaggacaa gaagtttgga tcaatacaga attatacgat aaatttttgt
    17581 ctttcaaatc ctataacagg ttacgcacaa gaaaggtaac acctaaagaa tttatcgaga
    17641 agcatttagt tgatttataa aaaacagaac attttgagag gtgaaggtta atgggtaaat
    17701 tcaacagagc attggtattc agcgcaccgc taatcatcta cgctttagga ctttggggaa
    17761 gcaggcaagc gttgatagga acgattgttt acatggtttg gatttttatg gggcttgatg
    17821 aagctgaagc tgagtacaga gcgaaaaagc caaccgaggg ggctgactaa aaatgaatag
    17881 aaaagagaaa ctagaatgta tattactatt actcagttta attctttcac taatttctct
    17941 attgggaagt ttgtattttt gatatcaaca attttgtgga tttgggttct atttagagta
    18001 aatatcattt ttgtttggtg attaaggagt ttattaaaat taattttccc tacttcaaat
    18061 gcaaggtaac aagatatagc acttttagga ggaatattta gcggtacagc ttcagagtat
    18121 attctattat catcgactga tcctccttta gcaattaatt tcttaaatgg agtagctgac
    18181 catctgcggt ttaagttagc ggatgaaagt tctaaattaa ctaacatagc tggttctgtt
    18241 gaatagttag aaatgattac ttttgtataa atcatatctg cagcgaagta agaagcatta
    18301 agttcaactt ctagttgagg gcgcattcct tttattttta tccctgttaa aaacgtactc
    18361 aatataaaac ttattattga catccattga aaaattgtta agtgaaaaaa attcaaaata
    18421 aacaccacca gtttttaact aaattatacc aaaaaggaga gaagaaataa tgcaagaatt
    18481 agtaattttg aaaaataaag aagctgtgac tacgagctta caagtggcag aaagcttcga
    18541 gaaaaaacat caacatgttt taagagatat tgatgcacta aaaaaagatg tgtccaattt
    18601 tggacagatg tttgtagaag gtaatgaacc agattcatat ggcagaaatc gacgagtttt
    18661 cttcattagt agagatggat ttttcttgct ggctatgggg tttacaggaa agaaagctat
    18721 ctacttcaaa caaaaataca ttgaagcatt caacgaaatg gaagatgtta ttcgcaagaa
    18781 tactgttcct caaacaattg aagatatgat gatctatcaa ttagaagaaa tgaaagatgt
    18841 taaaaaagat gtttccatgc ttaaagatac tatgcgaatt agcggacaac aagagtttga
    18901 aattaagcaa aaaggaaata tgaaagttat gggagttcta gggggaaaag aaagccgagc
    18961 ttatgaagaa atcagcaaaa aagtattctc aaaattttgg tctgaattta aacgtacctt
    19021 ttcaatccca agatatggcg agttacctcg taagagattc gatgatgctg tttcatttat
    19081 tgaaatgtgg ttgccagaaa ctgcgatccg tatggaaatt gatcaactga acagacaaca
    19141 gagacttttt ggtgatgaca atgaatagag ctgaagcgct aagaataggg acggcaattg
    19201 ctaattgctg gtggaaatac tataaaccaa tcatcctaag ccaacaacat attgacaagc
    19261 aaaaagcatg gcaacaaata aaaaagtgac tccgccggca agcaaagagt cacaaagaaa
    19321 acacatcata aggagatttt agcatatgga aaaagaactt tccactctag atcaatattt
    19381 gattgatcct gattggggca agccgaaaat tgaggaaaca agtggtcgaa aaatcagacg
    19441 aaatcttttg acgaatgaag aactagcttg ggatcaagat gatttaggca accatgtaac
    19501 tatttgggat catgtttatc ttatccatct atcgaagcat tcgaataaac ctgaatatat
    19561 ttacgtcatc gaagatggct tgattgatgc gctagaagag tacgacagag ataacttgat
    19621 tgatatctct tattacggac caggtaagaa atacattgct gaaatggagg cagaatttga
    19681 tgagtgaaat caaagggaca acgaactttg aaaaactttt tagtcgtaag ttaaataaaa
    19741 ttctcaagaa aaaaggaaat tttgattatt tatcttgggc tcacgcgtgg gagattatga
    19801 aaaagaatga tccacaggca acggtaacta ttaatgagta caaacactac agggttgttt
    19861 ctggaactca tcaagacttt cttgttgagg aatataaacc ttttcttatg gatgaaactg
    19921 ggacttatgt atctgtctca gtaacggtta aaggacacac ggaaaccga
//
LOCUS       DAZULO010000048        19820 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000048 DAZULO010000000
VERSION     DAZULO010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.049"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG8OA_001669"
     CDS             6..305
                     /locus_tag="ACG8OA_001669"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4976446.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG8OA_001670"
     CDS             378..1739
                     /locus_tag="ACG8OA_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFS4976447.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG8OA_001671"
     CDS             1761..2021
                     /locus_tag="ACG8OA_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976448.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG8OA_001672"
     CDS             2035..2787
                     /locus_tag="ACG8OA_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFS4976449.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG8OA_001673"
     CDS             complement(2881..3486)
                     /locus_tag="ACG8OA_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4976450.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG8OA_001674"
     CDS             complement(3539..4513)
                     /locus_tag="ACG8OA_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFS4976451.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG8OA_001675"
     CDS             complement(4891..5610)
                     /locus_tag="ACG8OA_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4976452.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8OA_001676"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8OA_001676"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS4976453.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG8OA_001677"
     CDS             7161..8558
                     /locus_tag="ACG8OA_001677"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4976454.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG8OA_001678"
     CDS             8639..9079
                     /locus_tag="ACG8OA_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4976455.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG8OA_001679"
     CDS             9227..9553
                     /locus_tag="ACG8OA_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4976456.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG8OA_001680"
     CDS             9554..10303
                     /locus_tag="ACG8OA_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFS4976457.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACG8OA_001681"
     CDS             complement(10383..10838)
                     /locus_tag="ACG8OA_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4976458.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACG8OA_001682"
     CDS             complement(11265..11540)
                     /locus_tag="ACG8OA_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976459.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="ACG8OA_001683"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="ACG8OA_001683"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFS4976460.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACG8OA_001684"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACG8OA_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS4976461.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="ACG8OA_001685"
     CDS             14770..17436
                     /locus_tag="ACG8OA_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4976462.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="ACG8OA_001686"
     CDS             17800..18552
                     /locus_tag="ACG8OA_001686"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4976463.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="ACG8OA_001687"
     CDS             complement(18574..19728)
                     /locus_tag="ACG8OA_001687"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFS4976464.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT         6329 a         3472 c         4060 g         5959 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aatccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa atggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat
//
LOCUS       DAZULO010000049        19242 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000049 DAZULO010000000
VERSION     DAZULO010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.050"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            162..1589
                     /locus_tag="ACG8OA_001688"
     CDS             162..1589
                     /locus_tag="ACG8OA_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4976465.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1681..2046)
                     /locus_tag="ACG8OA_001689"
     CDS             complement(1681..2046)
                     /locus_tag="ACG8OA_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS4976466.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(2043..2408)
                     /locus_tag="ACG8OA_001690"
     CDS             complement(2043..2408)
                     /locus_tag="ACG8OA_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS4976467.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2600..2848)
                     /locus_tag="ACG8OA_001691"
     CDS             complement(2600..2848)
                     /locus_tag="ACG8OA_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976468.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2847..2975
                     /locus_tag="ACG8OA_001692"
     CDS             2847..2975
                     /locus_tag="ACG8OA_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFS4976469.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            3032..4726
                     /locus_tag="ACG8OA_001693"
     CDS             3032..4726
                     /locus_tag="ACG8OA_001693"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFS4976470.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4793..4999
                     /locus_tag="ACG8OA_001694"
     CDS             4793..4999
                     /locus_tag="ACG8OA_001694"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976471.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5377..6510
                     /locus_tag="ACG8OA_001695"
     CDS             5377..6510
                     /locus_tag="ACG8OA_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFS4976472.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6541..6861
                     /locus_tag="ACG8OA_001696"
     CDS             6541..6861
                     /locus_tag="ACG8OA_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFS4976473.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6890..7354)
                     /locus_tag="ACG8OA_001697"
     CDS             complement(6890..7354)
                     /locus_tag="ACG8OA_001697"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4976474.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7486..8739)
                     /locus_tag="ACG8OA_001698"
     CDS             complement(7486..8739)
                     /locus_tag="ACG8OA_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4976475.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8768..9085)
                     /locus_tag="ACG8OA_001699"
     CDS             complement(8768..9085)
                     /locus_tag="ACG8OA_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4976476.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(9111..9431)
                     /locus_tag="ACG8OA_001700"
     CDS             complement(9111..9431)
                     /locus_tag="ACG8OA_001700"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4976477.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9895..11310)
                     /locus_tag="ACG8OA_001701"
     CDS             complement(9895..11310)
                     /locus_tag="ACG8OA_001701"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4976478.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11327..12643)
                     /locus_tag="ACG8OA_001702"
     CDS             complement(11327..12643)
                     /locus_tag="ACG8OA_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4976479.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12793..13491)
                     /locus_tag="ACG8OA_001703"
     CDS             complement(12793..13491)
                     /locus_tag="ACG8OA_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFS4976480.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13780..14769)
                     /locus_tag="ACG8OA_001704"
     CDS             complement(13780..14769)
                     /locus_tag="ACG8OA_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4976481.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14849..15682)
                     /gene="msrA"
                     /locus_tag="ACG8OA_001705"
     CDS             complement(14849..15682)
                     /gene="msrA"
                     /locus_tag="ACG8OA_001705"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4976482.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15857..17491)
                     /locus_tag="ACG8OA_001706"
     CDS             complement(15857..17491)
                     /locus_tag="ACG8OA_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFS4976483.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17478..>19242)
                     /locus_tag="ACG8OA_001707"
     CDS             complement(17478..>19242)
                     /locus_tag="ACG8OA_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976484.1"
                     /translation="LTQNLLKKDNSSILKIELKKENNKSMKISKKAFLLVVLILLSTL
                     YSVNFMRNAQEIYTTGDLSFHLSRIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTII
                     PAVVFYGISNNLILSYVLYIWLLNICTILISFYYGEKFFKKIDAAFLFSCLYTFATYR
                     TVDIYYRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLC
                     SALIACMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHS
                     PFKTDLQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVG
                     VVSTWVCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSW
                     LIIGATVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEA
                     IKERPHLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNG
                     KVVNDSYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAP
                     NKLKDSRYVTKKEINKKNENK"
BASE COUNT         6112 a         3758 c         3194 g         6178 t
ORIGIN      
        1 attttttaat agaattatct gttttccaaa ttttataaca taaaaaacct tttttaacgg
       61 catttatatg ctttttcaga aattaactga tgatttcaaa aagaaaacat gtcataatat
      121 ttcccatatt gtgcaaaaaa atacaaggcg gaggctctac tatgattgaa gaatatattg
      181 agaaaaatat tttaagacag ctgtttttat gtgggcaatt ctatgtaaat aaagaagtca
      241 atctagaaaa gctctccaat ctcctgcatg tttgcaaaac cactttactc aatgatatca
      301 ataacataaa aaaagaattt gaagaacaga tcgcttatac acatcgggaa aaggaccgtt
      361 acactttata tttttcagaa catatacctc gatgcaagat catgcagcaa ttgtctcaaa
      421 attcgttgtt tttaaaaact tgtctgcttt atttggaaga agatgaacct gattatcttc
      481 aattaactga atgtgaattc atctctgtca gcaaagctta ttcattaaaa aaacaagtat
      541 tagcttactt taatgactgc gggatcgaaa tcgaccgcta ctcccctcga ttcactgaaa
      601 tggaaagacg tctgctgctt cttaatgtgt cgtatcgttt aggcggtttt aattcttggg
      661 agctacctga atcgtttttt gaacgtgccg atcgtttcat tgaatccgta acggaaaata
      721 gtggcagatt ttatgataaa gaaaataaag aaatcttatc gattggtttt gctatcagtt
      781 tcctacgcca gcaagtgtgt gctgtgacaa tagattcaaa atttatcgaa gaaatcaaaa
      841 aacgtcctat ttacaattat gtcgaatcag catgggaaaa cactgatttc caaacctatt
      901 ataaaaaaga agaattcgcc tttattttga cactattcaa tctttgtaat tatggtttcc
      961 attcttatca gttgattgcg gaagactttc aacagctaca ccaagtgttt attgacaata
     1021 ctcccgaaat aaaagaacta gttgctacct ttgaatctca cttcaatcaa gaattattcg
     1081 gaaaccaacc gttcgaacgt gcgctgattc atctgatgcg ttcggcttgg gataactatc
     1141 aattattcat gccagaaaaa ttttatcttt taaatgagga acagacaaat ttatataaag
     1201 aagtccagac aattttttcc agctggtcca gccagttacc ttatgatcta cggttgaatc
     1261 ctaattgcat gcgggctttt gtgatcgaat tgagcggaat tctccgtctg acaaaagaac
     1321 atctgactat ctatatcgtc acaaattcgg atgttcatta tctgatttat cgggaagcct
     1381 tggaagctgt aactactttc gattttcaag ttgcaccaac aatttattca agtatatctg
     1441 atataaagaa gtatgcgcag cagtcctcaa accgggtatt atgtgaacgt acattatata
     1501 caccagatgc ggtccaatat gaaaatatta ttcctatttc gatcaatacg atcgatcgag
     1561 ctatcatttc agctgtgcaa aacaaataag gggctgtgac aaaaatcatg tcacagcctc
     1621 ttatttgttg aagatgccca tttctgtaac aatctcttga tacacgtcag gctcacaacc
     1681 tcacttattt tggacatcta ggggtccgat ctctttattt aaaaaataag aaatgatggt
     1741 tcggataact accaacgccg ctagttggaa aatatcctgc aaggtaggtt tgataatcga
     1801 ttcgatgatc ccagctgaaa caagaatttc caaacttaga agtatatagc ctcccaacat
     1861 ctttttaata gcattgttct cgcttactcg gtcgctttgc acttttgtat ggacaaataa
     1921 atatttcaaa aaatccttga ttgatagaca aacaccccaa accaataaac agatagcaaa
     1981 gatatcaagt cccagtacaa gtttttcgaa atatggaaga agaaactgca tcaactctgt
     2041 cgtcatccta cctctcctct tctttgtttt tctttgtctt ctatttcctg attcaagaaa
     2101 taagaaatga ctgttcgaat gataataata agtgcaagct tcaaaatatc tgtccaagtc
     2161 ggcttgataa ctgactcgat gatatcagca acgactagta cttccaagct taacaaaata
     2221 taacttgcta gcagttttct tgtatctgca ttacgagtat tacgttctgc atagtttttc
     2281 ttgacatgag accaatcaaa aagtttgaat agaacaagaa cgatcccgac aattaagata
     2341 ccgatagaac aaatatttaa aatagcaatg atccattcta gaaattcaat taaatttgag
     2401 cgaaacatat acgctaccta ctttctttta atttgttcct attcttttat tgtaaaagaa
     2461 agttaccttg ttcgtctatt taaaagttgt tctaatgata acggttcgct taaatttttc
     2521 tttttcactc tcccattttt ctccctccat ccataaatcg tggaagtatc caaaacaacg
     2581 tagcaagtta aaataaggct tagtccgtca tcagcttttc aaagaagcac tgtctcggtg
     2641 ctttttttgt acccaaattt ctaaaaatag aaccatacac ctcctcaaat cttttcttct
     2701 tacctaccct atttttattt acaggaaaat cagacacttc ttcttttttg tcaaaatttc
     2761 ctttttcttc tcgtcctatc atttggcatt ctaataaaaa cgaactatcc ttggatatag
     2821 aaagagaaaa caaggaggcg tttttcatga gtaaaaaatt aatcaaagtc ggcatcggtt
     2881 taggtttgct tgcccttggc gctgcatatc taggaaaaaa gacaggacta ttcgaagatg
     2941 acagtcattt atatgatgaa ttcgaatcaa tctagcatcc tatttagttg aatcactacg
     3001 gacatacttg gaagacattg gaggaatcac aatgagaaaa agtaaagcaa taatgattgg
     3061 tgccggtatt gccaatatgg cagcggctgt ttatttgatt caagaaggca aatggaaagg
     3121 ggatcagatc accttttatt cattggatga tcacggttca aatgatggtg caccgaccga
     3181 cactgtagtg gatgaatatt ggaataaaaa ccatccatta gaaaaccaaa aaggctatat
     3241 tgctcgaggc ggtcgcatgc ttaattaccg tacctatgta gatttgatgg atctgttaag
     3301 tcgaatacct tctgttactg agccagggat gactgctgaa gaagatacga gagattttga
     3361 tgcaaaacac cgaacctttg acaaagcacg tttgatggaa ggtggcaaag gaatcattga
     3421 tggcggaaaa ctgggattca ataataaaga ccgtattctt ctgactaaac tgattgctat
     3481 gcctgattca gaagaagaaa aactagacaa tatcactatc gaagaatatt tcaaagatga
     3541 tccacacttt tttgaaacaa acttctggtt tatgtgggaa acaacatttg ctttcagaac
     3601 acgtagttct gctcaagaat tgcgtcgcta tatgcatcaa atgatttatg aattcactca
     3661 aatcgaacat ctagtgggcg tcaatcgaac acgttacaat caatacgaaa gtattatgct
     3721 tcctttgatc gagtacttga aagaacaagg atgcaaaatc attttgaacc gccgtgtcac
     3781 tgactgggaa ttcaaagaaa caccgatgca agatgaaatc actgtgactg gtttgaaaat
     3841 taccaataca ctgactcaag aagaagaata cgttgtggtc gatgaagata cagcagttat
     3901 tttcactaat gggtccatta cagattcagc tactttagga gattacgaaa caccagctgt
     3961 agaaaatatg gattatggtg cagcttctag tctctggaaa aaagccagtg aacgtttcta
     4021 caacttggga aatcctgaca aattctttgc tgatcgagat gcaagcgaat gggtcagctt
     4081 taccctgacg acgaaaaacc atttgttgtt gaatgaaatc acccgtatca cgactcaaga
     4141 accaggaaat gccttgaatt catttatttc aaccacaccg atcacgccgt taggccaaaa
     4201 agatgtcaat atgtctattg tcgtgcatca tcaaccgcat tttacgtctc agaaaccaaa
     4261 tgaaacagtt atttggggtt atttccttta tccaagacga cgtggcgaat ttgtggataa
     4321 acagtatatt aaaatgaatg gaaaagaaat gctagaagaa ctgattggac aactttcaaa
     4381 agtagatcct ggaccagtga atattcgtga aaaagaaaca gaaattttcg acagcgtaat
     4441 aaataatatc cctgtctata tgccttatgc ttccgcgttg ttcaataatc gtgccaaaag
     4501 cgatcgacca aaagtcattc ctaaacattc gaccaatctg gcctttacag gagaatttgt
     4561 cgagcagcct tatcagatga tctttactga acaaagtgct gtacgttctg gagaaatcgc
     4621 tgctttccat tttgcgggta ttcctatgtc ccgtcttgtc aaaacaccgc gttacgacaa
     4681 agatattcct actctagctc gtgccgcaaa aaaaatgttt gaataatcaa gaggttgtga
     4741 aaaagctgcc ctgcatcaag taataagaac aaccaggcta aaacagcttc ttatgttttt
     4801 gatttggttg gacttattac cgcagatgca agcttttgaa caccgtttac caagaggtta
     4861 tgaaaaagct gccctgcatc aagtaataag agcagccaag ctaaaacagc ttcttatgtt
     4921 tttgacttgg ttagacttat taccgcagat gcaagctttt gaacaccgtt taccaagagg
     4981 ttatgaaaaa ggcgtttagt ttcgagtatg aagaaaaatt ttgtgaaaat ggttcctcac
     5041 gtttttgcat aaatttgact taataccgag aaactgatcc cttctgtcat aacctctttt
     5101 tatttatgtc tgtttctttt tgaaaacaac tattttttca acgtttactc tcttgccaat
     5161 ccaacatcat ctaacggata caactatttc aatgattacc attacactaa aaaatcggaa
     5221 acacacctct ccgaacacaa atacataaga ctatagaatg tttcatttaa gaaaaaaagc
     5281 gtttcattat caaaaaggta agatctcctt taaaatgaaa ttatctgtag tactaataat
     5341 gcagacaatt ttatgaatct gaaaggagca tctccaatga aagcagtcac ttggcaagga
     5401 aaacagaaaa tggaaatcag aaacgtagat gatccaaaaa tattagaacc aacagatgca
     5461 atcatccgta tcacagcaac agccatttgc ggctccgatc tgcatcttta ccatcatggt
     5521 gaagcagttt tggaaaaagg gtatgttgta ggacacgaac caatgggaat cgttgaagaa
     5581 gtgggatctc aagttcaaac attgaaaaaa gggcaacgtg tggttatccc ttttaatatt
     5641 agttgtggtc attgtcattt ctgcctgaat cacatggaaa gccaatgtga tgaatcaaat
     5701 ccagatcagt tatatggcgg gcttttcgga tttggcaagt tgaacggtaa tcattggggt
     5761 ggtcaagcgg aatatctaag agttccttat gcagactcta cttcatttgt tgtaccagat
     5821 aatgatcttc ctgatgaaaa agttttattt ctatcagata ttttgcccac agcttattgg
     5881 agcgttgaaa acgcaggagt aaaaaaaggc gatacagttg tgattctagg ttctggtcca
     5941 gtcggtctat tcgctcaaaa attcgctgtt atggctggag cagagcgcgt aatcgcagta
     6001 gatccagtac agcaccgtct agataaagcg gctaattaca atggggttga aacttatttc
     6061 ttagaagaca cggcacaagc tggaacagaa ttgtatgaac tggctaaagg tggcgcagat
     6121 gtcattatcg attgtgtagg aatggacgga ttagagcctg tcaaagaaaa agcgaaaaat
     6181 ttagtaagct tacaaagcgg cacaatctct ccattacaga tggcatcgca agctgtcaaa
     6241 aaatttggaa cagtccaagt aactggtgtt tatatgactc cagcttcttc ttacccgctg
     6301 caagaatttt ttatgcggga tatcgctgtc aagcatggac aggcaccaat tatccatcta
     6361 atgccaaaaa tttacgcaat gattgcagaa ggactattcg atcctacaca aatcattact
     6421 cattcgctgc cactatcaga agcagcacaa gcatatgaga tctttgacaa aaaagaagat
     6481 aaaaatataa aagttgtttt gaaaccttga attcagtaaa aaatacaggt gattttcttt
     6541 atgcccaaaa tcaaacgaaa tacccatgag atcgaaatag gaaaagaaga agatatcgaa
     6601 atacgaggac gtcgcttccg cttctatttc caaaatcgtt acacattgat ctcactggct
     6661 gtcgatttat taaccgggat tttctatatt tttggaagcc ttgcttcctt aactaaaatc
     6721 cccgatcgat atggcaccta tttttatttg gctggtgcta ttttcttgac gattcgtcct
     6781 attctcagaa tcgtaaggaa catttttatt tacgacgaaa aaaaagcaat gaagagaaca
     6841 agcaaaaaaa aagaggcatg agaatttttc aaatctcatg cctctttttt caccctatcg
     6901 tatcactttg atacagacga gcggtcgtcc cactttcttc cttgaccatt gtcaaaaatg
     6961 tccactcgta tcgttcataa aacgtggtat gatctgtaat taagtaaagt ttttcgtacc
     7021 caagttgatg catttctttt ctgatttccg ctaatatccg cttggctatc ccctgcttac
     7081 gcttttccgg ttcaacaaac agggcacaaa gatttggtgt cagatcaggg cggttatgga
     7141 agtcgttttc aatgacgccc gctcctccga cgatggtttc tttttcatct aagaccacat
     7201 accattgagg aatactttcc ttattagcga ttccagtacg aatactttcc tcatacacaa
     7261 cagcaggaac tccccatttt gatgaaaacc aattcgcgct ttgttttact aattctgggt
     7321 gttctcttaa tttgatccag ttatatgtaa ccaatgttta aacctccgac taaaataaaa
     7381 cgtttacaaa aattatacaa gtctttatga aaattagcaa tgacaacaat agtgatgtaa
     7441 aaaaaaaaac ggaaaacaat cacttgttct ccgttttttg attaatcaag catctaaagc
     7501 ttcttttctt ctttcttgga tcgtagctac cttgacgaac ggtagataga tcaaaataga
     7561 aagaaccaaa ttgacagcgg ctagtacacc acctgcaata ctttttgttg ccaatacacc
     7621 gccaataatt ggtggagtta cccatggagg cataacggta gctgctggca ctaaacctgt
     7681 agaagtcgca aagtaagcaa cactaactaa gaccattggt gtcaaaataa atggaatgaa
     7741 catgatcggg ttcaacacga taggtaaccc aaacatcgtt ggttcattaa tattgaagac
     7801 acctggcgca atcgacaagt ttgttacaac catatatggt ttgtttttac gtcctactaa
     7861 ataaatagct aacagtaaac ctaaagttgc acctgttcct cctaagttga caaagctgtc
     7921 aaagaaaggt ttgttcacga taaacggtgc gctatgtcca gctgcgattg cattaacgtt
     7981 tgcttcaatg gctggcgcat tgattgtttg catcaaagga tcgaccatat tcgctccgtg
     8041 taaaccaaag aaccataaga atggtgtgat aaatgcaagc agtaaggcag atggataaga
     8101 gtttgcaagt cccataaatg gttcttggac cactgtatag aaagaaccta cgatatcatc
     8161 aacaccgaat gcagcaaaaa ttgcagcaac taaagcaaac gcagagatag tgatcattgc
     8221 tggtagtaat gaagcaaatg cttttgcaac tgccggtggc acaccatcag gcattttgat
     8281 gatcaatttt tcatttccgc ttaaacgtac aaagatttct gttgaaataa tcccaacaat
     8341 aatagcaata aacaaaccgt ttgagctcat acctaatgct ccgcctaaag cgaagaaaga
     8401 gcctaaagaa atcacacctg cggaaacacc gtcttttcgg tagccgttag ctaaatgata
     8461 agcaagcaag aaagcaatca agacagaaag aatggcaaat gtaccgttcc aagcagcagc
     8521 cccgaatcct ttccatgatt caccaccaaa aattgagttc atcaattctt ggaacccggg
     8581 aatcggtaag ttattgatca ttactgctaa tgctcctaag atcattaacg gcatactcac
     8641 cataaatgag tcccgaatcg caactaaatg ccgttgtgca ccaatttttg atgctacagg
     8701 tataaaatgt ttctccataa atgcaacaaa tttatccata aatcttccct cttttcttta
     8761 ctgtagatta cgcgttcaat cgacgataca gattaatgat ctcttttgct aaatctgtaa
     8821 aagctataga tgtcatcaga tgatcttgac tgtggaccgt caaaagcgtg acttcaacgt
     8881 gatttccttg tgcttcttgt gtcagcatct ctgtttgaga gtgatgtgct tggactaaag
     8941 attcctctgc atctttgatt ttttcatctg ctaaagtgaa atcacctttt ttcgcagcag
     9001 caatcgcttc cattgcatcg ctttttgcat ttccgccaaa catgatcaga cccatgatgg
     9061 cttctagatt tttttgatct tccatttatt gtatcctccg ataattttta ttatttgttt
     9121 attagttttt ctgcttggtc gagtactttt tcaccattca tcatgccata gtcagccata
     9181 ttgattacat ctaaaggaat gcctttaggt gctagctttt cagcaaattg attttgcata
     9241 aagcgtactt gcggtcctaa aagtaaaacg tctacatttt tagattctag ttgattatcc
     9301 gcatctgaag cagatacagc aaagatgtct gcttccattc cttttgcttc tgctgccttt
     9361 tgcatttttg tgactaggag acttgtactc attccagctg aacatacaag catgattgtt
     9421 ttcttttcca ttttaaacac gttcctttct atacttacac ttgtatataa gcaagtagcg
     9481 tgccaacttt aaaagcgctt taaaaagttg atatcatcac atttaaggtc tataccaagt
     9541 tgcatacaca ctaaaaaaga aagcgcacac taaacacaca aattaatttt tattaccttt
     9601 tttcttttaa cgtgcacaaa aaaagttgtg acacaaaact tgtcacaact tttttcaaat
     9661 aaacggtatt caaaagcttg catctgcggt aataagtcca accaagttaa aaacataaga
     9721 tgttgttttt actttgctat tcttattact tgatgcagga cagctttttc acaacctctc
     9781 aataaacggt gttcaatcag ctgaccctgg gtattagctc aaaacgctgg attcacaacc
     9841 tcttttattt caaataacca gtaaatacaa tgaacatcta caaaaaactt ctatttattt
     9901 atagatttcg tcaggcaaat ccaattctct cttatcaatc aaagtctgat accacgaagc
     9961 agattttttc aatgttctgc ttcgttcatc gttcaattcg attcgaacta atccatatcg
    10021 attcttaaat gcattcattg gtgacacaca gtcagtgaat gcccataaca tatagccttc
    10081 gcaatttgcg ccttcttctg ttgctttgat caagtaatgc aaatgctgtc cgataaattc
    10141 gatacggtaa tcatcttgca cgattccttt ctcatccata tactgttctt catattctct
    10201 tcccataccg ttttctgtta tcagccaagg gatattatga taattttctt ttagatacat
    10261 ggctatatca tacatgattt tcggatagat ttcccatccc ctggaattat tcattttctt
    10321 cccaggtaaa tcaaagactt cataatatcg ttcaggatgg aaaggtgtct cttcattcca
    10381 ttgataacga ggggcgcgaa cgcgtttagg ataatattga ttgattccaa ggaaatctac
    10441 tgtatgatgc tgaatcgttt ctaaatcctc tgttgcagga tcgaaataaa tcgattgttt
    10501 ttgacacaac tccaggaact cttctgtgta gtatcctttt accatcggat caaaaaatac
    10561 tcgattataa aacaaatcat acatataagc agctttttgg tcttctggag aattggatcg
    10621 agcatagacc atttcaggat ttaaaataca gccaatcctt ccaccataat ttccgctgcg
    10681 gtaaatctct actgctttag ccgttgctaa aactttatga taattccaca tcatccactt
    10741 ttttgtgttt tgttcatgcg gccagcgaac agcatccaaa tagcaacgcg tttggggaac
    10801 gattggttcg ttgaatgtaa accagttttt taccctatca ccgtaacgat caaaggcaat
    10861 ttgagcatac ttagcataaa aatcaactac ttttcttgaa gaccagccat catatttttc
    10921 caaaagaaaa gctgggactt cataatgttc caaacaaatc atcggttcta cgtctgcttc
    10981 gatcaaagag tcgatcattt cacttatatg ctgagcatat tcttcatcta ctatacagtt
    11041 ctcataatca gtaaaaaaac gagcccaatt gatagaagtc cgataatgtg tcagatggat
    11101 ggatttcatc agatccacat cctgctggta ttgttccata aaattagttg ctacggctgg
    11161 gccataccca ttatgccaaa caaaatgttc atttttatac cagctatcta taaacgaatc
    11221 ctgtccttct ttttttcctt tccaaccttc cgtttgccaa gcagaagatg cagcaccaag
    11281 aataaaattt tttggaatag taattttcat gatttcctcc tgttttttag atcccatcat
    11341 tttccacacg atttgctact tttacaaatg gcagatagat caaaatagaa acaataatac
    11401 aaacaagttg cgtaataact gcaccgatat ttcctgctgt tgccagataa gcattgacga
    11461 tcggcggcat tggccaaggg accattacaa ctgctttcgc cgcaaatcct aaagcggttg
    11521 caaaataacc aatcgttcca gtgataagag gcgttaagat aaacggaata gctaaaattg
    11581 gattcagcat gatcggcata ccaaaaataa ctggttcatt gatgttgaag atcccaggag
    11641 caagggataa cttggtgatt tgtttcatat cttcccgttt tccaactaac aatactgcaa
    11701 tcagtaaacc gatcgtgact ccagacccgc ccatactcat atacatatcc caaaatggca
    11761 tagtaataat gttcggtact tcttttccag cttcaaaagc ttccgtattt acagcaattg
    11821 ccgctaaaag caacggttcc cgtatcggct tcaccatttg atttccatgg ataccaatca
    11881 cccaaaagac ttgcgcaata aacatcaata gcaaaatacc cggtaaccct tgtacgacac
    11941 cttctaatgg gcgttgtacg acattgtaaa tgatatcata aaggtacatt cctgtaagtt
    12001 ctttgatgat aaatcccgca gccgcgatac ccgatacagt cagaatcgtc gggaataaag
    12061 cagaaaaact agcagaaaca ttcgttggca cggtatcagg cattctcagc tgtagtttct
    12121 tctgtttcct caaccagcaa taaagttcaa tagaaaggat ggagaccaac attcccaaaa
    12181 ataacccttt cgtatctgta tattgtttag ccaatacgtt ttctactata acagtctgat
    12241 tttcattcac taattcataa acagttggat taacagataa ataagacatc actgcgatga
    12301 taccagggaa tacaccatcc actttattca gcttggctaa ttcgatccct atcagaaaaa
    12361 cagcatagat ggtcaagaaa cttagtgtga catagctgat ggcagatgcg ataggcttta
    12421 attcttctaa aaaacgcaag aaacttatct gtgctaagcc attttccgag tcaaagacca
    12481 ttgctgaaaa caatgtgcca aaagcacccg taataattac cggaatcaat gcagcaaatg
    12541 cgttttttat caccatgata taccttaaat tattgattct cgttgccacg ttccctaaaa
    12601 cattggctaa tttatccata aaactcattc cagatcccct cattcctgct tcttctcctt
    12661 gacgtcatct ctttttgtct attagtcagc gcttacattt cgatgaacag aaactattta
    12721 ctcctctttt ttaccaaata taaaacaaaa attcatctga tgtatttatc ataaaataca
    12781 tcagatgaac tgtcaattag tttctttttc tcctattatt ttctgtaatt ctctccgatt
    12841 tattgccata tggacagtca ttgcatcaaa cgcagcaagt gcattccgcc tttcgatggc
    12901 ctgaacgatc tctgtatgaa tatcgatcgt ctcgattttc agatgctgga tatcataatt
    12961 tttattaaac aaagcaattg attcattaat gattgggata atatgcagca aggctacgtt
    13021 tccgctagct tctgcaatca tcgtatgaaa atcgatgtct aattgaacat gttcttgtgt
    13081 gccttctcga aaagaagttt caatcttttg cgttaatttt ttcatttgga cgatttgatt
    13141 cttcgttgca tattccgctg ccaaacctgc catcttaggc tccaataaat atctcagttc
    13201 aaataagtct cgtatcatcc gatcctgatc ttcaatcaaa gaaaaaccga gtgggtcatt
    13261 ggcaatccct cgcttatcac tgacaaatgt ccccgctcct tgccgaactt caaggatatt
    13321 cctggaagct agagcccgca cagcttctcg caccgtgctt cgccccacct ctaaataatc
    13381 ggacaattca tattcattcg gcagtttatc tcctacttgc aactgcattt cctttaaata
    13441 tctaaggatc ttgtcagtag ttacttctac tagggtttta ttttcttcca aaccacatac
    13501 ctccttttca tctgatgtat tttttcgtaa ttattataac acgtttgaat catctgatga
    13561 ttttttgtat acaaaattga ttaatccgtt catagacata caaacgaaga agatatttac
    13621 cataaaaaag actaggataa aattcactcc tagccttcga tcatttttat tttgaacgac
    13681 aaaaaatatg atgttcaata acagacgatc ttcattcgat tcttgatgtc atcttttgtt
    13741 ttttttaata tatggataaa aagtttgatt acatcgtttc tattctagaa tttggtcgat
    13801 tctcaattct tcttctggtg agaaatctaa attatccatc attcgaacac tttcttgtaa
    13861 ctgacttaac cgacttgctc cgaccagtac acttgtaaca gatttttgtc gcaaattcca
    13921 tgcaagggcc atttgcgcta atgattgtcc tcgtgatacg gcgattgttt gcagtgcttt
    13981 gattttttcc agagttgatc caacttgttc ttctgaaagg aatggtattt ccttacgatg
    14041 tgctctagaa tcttcaggaa taccatgtag ataacgattt gtcagcaacc cttgagcaag
    14101 cggactgaac gtgattgccc ctagcccttc ttcttctaat actggagata gatcgtcttc
    14161 tagtagtgca cgagaaaaca tattgtagcg catctgatga ataatgaacg gtgcttcctt
    14221 tctttttaaa atttcagtca ttttttttgt atcttctcca ttatagttgg atataccaat
    14281 atataatgct tttccttgtc ttacaagttg catcaaagct tctgctgttt cttccaaagg
    14341 tgtatcaggg tctgggcgat gatgatagaa gatgtcaaca taatctagcc ccattctttg
    14401 gagactttgg tcacaacttg aaatgatgga tttacgagag ccccattccc catatggccc
    14461 ttcccacata tgataacctg ctttgctaga aataatcagt tcatcacggt aggcttgaaa
    14521 atcttctcta aaaatacgcc caaagttttt ctctgcagct ccagcagggc ctccataatt
    14581 attcgcaagg tcgaaatggg taattcccat gtcgaatgcg cctcttaaaa tctctctttg
    14641 attatggata ggatcataat caccaaaatt ttgccataat ccgagagata aaatcggtaa
    14701 tttcaaacct gaatgaccgg atttacgata ttccattttt tcataacgtg cactatctgc
    14761 aatatacaat ttttctcctc ctcatcaaat ctttttaaaa cgctttcttt tttctatttt
    14821 tagtttacta cattttaaag aaaaggagtt aatcaaaatg attttttcgt atattttttt
    14881 gccatttgtt ttttactcgt tgatagatca gaaattgatt acgtgcttgc tggatttttt
    14941 tataacgttt cggttgtttt ttgtaaaatt gctgatgata gttttctgct ggccaaaacg
    15001 taccggctgg ttcgattttt gtgacgattg gttggtggaa agttcctgaa ctgtctagtt
    15061 tctgttttga ttgttccgct aattcttttt gtcgttcgac ttgataaaaa atcactggac
    15121 gatattgctt ccctctatct tgaaattgtc caaatgtatc tgtgggatca gtaatttgcc
    15181 aatagattgt cagcaactcg ctataactta tttctcttgt gtcataaatg atctccacag
    15241 cttccacatg acctgtgtaa ccaccactta cttgatcata atttggtttt tcaacatgtc
    15301 cgcccgtgta tcctgacaaa acagatagga tccctttttt cgattcaaac ggctcgacca
    15361 tacaccagaa acatccccct gcaaatactg cacgttcttg atagtcagca tcttgcatgg
    15421 catgcttgga aaagtcatat ttttgctcct tatcaaatcc ttccgtaatc aataaataaa
    15481 agtccattac atcaggcgtc agattcattc ggatagctaa tggacgtagt gttgcttcta
    15541 gttttgataa ctgttcttta acggatatat tttctcttat gtcattcttt gtctgaatca
    15601 atacttttcg ttcccaatct cgtgtattcg gattcaaaat cagattataa agtgtttgaa
    15661 tgaatatttg ttcctcatcc atagattctc ctcctttccg aagaagacaa gctagtgact
    15721 ttagctgtaa tttatcataa agtgaggaaa taagcgatta cttcatcccc ctacagattt
    15781 ttcgtataaa aaagagagaa tccttatcga cagattctcc tttttaatca atttatttta
    15841 taaatgaaaa gttaatttac cttttatgga aaactatttt aaaaaataaa cataaatatg
    15901 cagttaatgt aacaagataa gaaataatag tcgttactgt ataacttgtg cttactttaa
    15961 tatcagcaac tcctttgact ccgcttattt gtaccaatcc attagcattc acctgctcct
    16021 ttacaggttg atgatttatt tctacattcg ttcctaaata agcatataca ggaagtgtga
    16081 ttacttggct cttctcatta gttactctat atatgataga actaccagtt gttttataag
    16141 gaaggtttac ccattgactg ccaatctttg cttcgtgttt tattatatgc tccttattct
    16201 ccaatgaagc gataggagaa taatcatata aaaaataatc cattccttta gtggtatacg
    16261 cctctactgt ttctttgttt acaacaatat gtcccggctg agaaattatt ttttgattga
    16321 aatttttttt actagaaaca gttaaagtga ttaataacaa aacagtcata aataaataaa
    16381 atagttctct tgtcttattg tatcttctat ttttgaaaaa tatcatcgtt aatatcagag
    16441 aagagaatat tatttgcaag cttaagatcc gccaaggaaa ttgtaacaat ttggctggcg
    16501 tattttgcag aagataccat ggcaccaaac tagttgtagc tagccaagtc aaacacgtac
    16561 cataataaat ctttttttct atgtttgtta attttttaaa taaaatcagg ggaatacata
    16621 aagataacag accaactagt cccattgagt aagtagttaa gtcgttgttc atagcttcag
    16681 tcagactatc gaacacatta gatgcatgag catataaatt tgtcacgcta ggtttgttga
    16741 ttttttggta gagcatttgt tcaaacatcg gataccaaaa agcactcgtc agaaccaatg
    16801 tcattcctgc cgcactaaat aattttttaa atatagcaat aaaatctgca ttcttgaaac
    16861 ctttagaaac caaacgaata aaaataaaaa tgataattaa tgctgtactc attaatgttg
    16921 atagtacatg gctgtacact agcaaactca tactcaatgc tagctttaca gacggatact
    16981 tttcttcttt tccagagatg atttgataag cataaaataa tacagggata acaagtgtta
    17041 ttgcgatagc ttcggcaatt gcggaacgat agtagatgtc tactgtccga taagcagaga
    17101 aaatatagag acaactaaat aaaaatgccg cttttttttg ttttaaaaac cgctcgccat
    17161 aatgataagc tacgagtgtt gtacacaagt tcaataacca aacgtaaata atatagctta
    17221 caatcaaatt ttttgtcatc caatataaca ttacagctgg aaaaaaagtc agaaagggat
    17281 aaaaataatt tacaccatat ccagtataat tgaatgtctc ataattaatc ggactggtaa
    17341 aaatagtcga taaccccttg attcgagata agtgaaaaga aagatctcca gtggtataaa
    17401 tactctgtgt atttttgaaa aagatgaaag aatatatact actaactata attaatccac
    17461 tcaatattac tactttttta tttattttca ttcttcttgt ttatctcctt tttagtcaca
    17521 tatcgagaat ctttcaactt attaggtgca aatcgtttaa aacttgaata caccactaaa
    17581 caataagtca gaatactgat ccataaagcc atgagagcag ttggagtata ttttgttgta
    17641 atttctatct tattttctcc agcatttaca tccacattca ctaagccatg ttttttataa
    17701 gagtcattta cgactttacc gtttactttt acctgtgtac ctaaataagc atatactggt
    17761 actgtcactt tttgcttttt gttgcttgcc actgtgtaag taatgttatt atctgcagct
    17821 ttatacttcc ctttttccca cgattctcct acttttactt catgtttttc taagtgaggc
    17881 ctttctttta tcgcttctgt tggggcgtaa tcgtataatc ctcccatatc tgatgttgta
    17941 taacgtggga gtgtttcttc attgataaca atatgaccgt gttttgattg gataactttt
    18001 gcgtaatttt cttttgctgc tatagacgtg acgagtagaa acaccacagt agcgccaatg
    18061 attaaccatg aatatttttt attcatagtc tttttcataa aaacaagcgt tatgatcatt
    18121 gaagaaaaaa agatttggat acctaagata cgccaaggaa actggagtaa gcttatcggt
    18181 gttttttgaa acaaaaacca tggaaataaa ttagttacgc atacccatgt agaaactacg
    18241 ccaacataga tgattttttc ttttttctcc atttctttca gtatgaataa aggaaaaatc
    18301 aacgcaacca atcctaataa ccccatagtg tatgttgtaa tatcgttatt taacgcttga
    18361 atgatactat ctgaaataga aagtgcttct agctgcaaat ctgttttgaa gggactgtgt
    18421 atttcttgat aattcatctg ctgcaacata ggataccaaa aatagctagt taatgcacaa
    18481 gtcaaaaaaa cagcctttac taaattcacg accacaaatt ttatatcgtt ttttatttgc
    18541 ttgcttaaaa acaaacgaac tataatcata caagcaatca atgcactaca caatagtgta
    18601 gttaagacat gagtatacac aagtagactt atactgacag ataaagcgat gacttttttt
    18661 tctctattga atatcaatcc ataagcataa tataaaacag ctgggataat cgtaagagcg
    18721 attgcttctg caattgcgga tcgataatag atatcaactg ttcgataagt tgcaaaagta
    18781 tataaacaac taaataaaaa agcagcatct atctttttaa aaaacttttc tccataataa
    18841 aaactaatca gaatcgtaca aatatttaat aaccaaatat atagaacata acttaatatt
    18901 aagttatttg aaattccata aaaaacgact gcaggaatta ttgtaagaaa aggataaaaa
    18961 tagttcagtc catctccata attgttaaat gttgtatagt tgattggccc ttcaaaaata
    19021 gaagataacc ctttgattcg tgaaagatga aaagataaat cacctgttgt ataaatttct
    19081 tgagcatttc tcataaaatt tactgaatat aaagtactaa gtaagataag aactactaaa
    19141 agaaatgctt ttttacttat tttcatagat ttgttgttct ccttttttaa ttctattttc
    19201 agtatactag aattgtcttt ttttaataag ttttgcgtta aa
//
LOCUS       DAZULO010000050        19126 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000050 DAZULO010000000
VERSION     DAZULO010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.051"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..703)
                     /locus_tag="ACG8OA_001708"
     CDS             complement(<1..703)
                     /locus_tag="ACG8OA_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4976485.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="ACG8OA_001709"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="ACG8OA_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="ACG8OA_001710"
     CDS             1758..2837
                     /locus_tag="ACG8OA_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS4976486.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2834..3462
                     /locus_tag="ACG8OA_001711"
                     /pseudo
     CDS             2834..3462
                     /locus_tag="ACG8OA_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(3572..3799)
                     /locus_tag="ACG8OA_001712"
     CDS             complement(3572..3799)
                     /locus_tag="ACG8OA_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4976487.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="ACG8OA_001713"
     CDS             complement(3902..6490)
                     /locus_tag="ACG8OA_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFS4976488.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="ACG8OA_001714"
     CDS             complement(6510..7169)
                     /locus_tag="ACG8OA_001714"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4976489.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="ACG8OA_001715"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="ACG8OA_001715"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFS4976490.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="ACG8OA_001716"
     CDS             complement(7942..8808)
                     /locus_tag="ACG8OA_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS4976491.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="ACG8OA_001717"
     CDS             8917..9558
                     /locus_tag="ACG8OA_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFS4976492.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="ACG8OA_001718"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="ACG8OA_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFS4976493.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="ACG8OA_001719"
     CDS             complement(10999..11898)
                     /locus_tag="ACG8OA_001719"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS4976494.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="ACG8OA_001720"
     CDS             complement(11900..12697)
                     /locus_tag="ACG8OA_001720"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFS4976495.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="ACG8OA_001721"
     CDS             complement(12675..13379)
                     /locus_tag="ACG8OA_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFS4976496.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="ACG8OA_001722"
     CDS             13510..14085
                     /locus_tag="ACG8OA_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFS4976497.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="ACG8OA_001723"
     CDS             14203..14880
                     /locus_tag="ACG8OA_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFS4976498.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="ACG8OA_001724"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="ACG8OA_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFS4976499.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="ACG8OA_001725"
     CDS             complement(16943..17941)
                     /locus_tag="ACG8OA_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFS4976500.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="ACG8OA_001726"
     CDS             complement(18167..18820)
                     /locus_tag="ACG8OA_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFS4976501.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5525 a         4136 c         3324 g         6141 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagt
//
LOCUS       DAZULO010000051        18733 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000051 DAZULO010000000
VERSION     DAZULO010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.052"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            256..531
                     /locus_tag="ACG8OA_001727"
     CDS             256..531
                     /locus_tag="ACG8OA_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976502.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="ACG8OA_001728"
     CDS             544..2616
                     /locus_tag="ACG8OA_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS4976503.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2784..4667
                     /locus_tag="ACG8OA_001729"
     CDS             2784..4667
                     /locus_tag="ACG8OA_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4976504.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            4679..5626
                     /locus_tag="ACG8OA_001730"
     CDS             4679..5626
                     /locus_tag="ACG8OA_001730"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFS4976505.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="ACG8OA_001731"
     CDS             5646..6173
                     /locus_tag="ACG8OA_001731"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS4976506.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="ACG8OA_001732"
     CDS             complement(6236..6889)
                     /locus_tag="ACG8OA_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFS4976507.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="ACG8OA_001733"
     CDS             7021..7863
                     /locus_tag="ACG8OA_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4976508.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="ACG8OA_001734"
     CDS             8021..8917
                     /locus_tag="ACG8OA_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS4976509.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="ACG8OA_001735"
     CDS             8920..9633
                     /locus_tag="ACG8OA_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFS4976510.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="ACG8OA_001736"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="ACG8OA_001736"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4976511.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="ACG8OA_001737"
     CDS             10168..10395
                     /locus_tag="ACG8OA_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFS4976512.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="ACG8OA_001738"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="ACG8OA_001738"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS4976513.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11723)
                     /locus_tag="ACG8OA_001739"
     CDS             complement(11181..11723)
                     /locus_tag="ACG8OA_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS4976514.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="ACG8OA_001740"
     CDS             complement(11960..12358)
                     /locus_tag="ACG8OA_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4976515.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="ACG8OA_001741"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="ACG8OA_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFS4976516.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="ACG8OA_001742"
     CDS             13471..14238
                     /locus_tag="ACG8OA_001742"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFS4976517.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="ACG8OA_001743"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="ACG8OA_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFS4976518.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="ACG8OA_001744"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="ACG8OA_001744"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFS4976519.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="ACG8OA_001745"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="ACG8OA_001745"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFS4976520.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3230 c         3899 g         5086 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcgttttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacatcggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgc
//
LOCUS       DAZULO010000052        18350 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000052 DAZULO010000000
VERSION     DAZULO010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.053"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..564)
                     /locus_tag="ACG8OA_001746"
     CDS             complement(109..564)
                     /locus_tag="ACG8OA_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976521.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="ACG8OA_001747"
     CDS             complement(718..1926)
                     /locus_tag="ACG8OA_001747"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFS4976522.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="ACG8OA_001748"
     CDS             complement(2038..2370)
                     /locus_tag="ACG8OA_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS4976523.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="ACG8OA_001749"
     CDS             2518..3393
                     /locus_tag="ACG8OA_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4976524.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="ACG8OA_001750"
     CDS             complement(3475..4056)
                     /locus_tag="ACG8OA_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFS4976525.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="ACG8OA_001751"
     CDS             complement(4062..5321)
                     /locus_tag="ACG8OA_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS4976526.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="ACG8OA_001752"
     CDS             complement(5323..6369)
                     /locus_tag="ACG8OA_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976527.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6604..6879)
                     /locus_tag="ACG8OA_001753"
     CDS             complement(6604..6879)
                     /locus_tag="ACG8OA_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFS4976528.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7122..8432)
                     /gene="der"
                     /locus_tag="ACG8OA_001754"
     CDS             complement(7122..8432)
                     /gene="der"
                     /locus_tag="ACG8OA_001754"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFS4976529.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="ACG8OA_001755"
     CDS             complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="ACG8OA_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFS4976530.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9978..10658)
                     /gene="cmk"
                     /locus_tag="ACG8OA_001756"
     CDS             complement(9978..10658)
                     /gene="cmk"
                     /locus_tag="ACG8OA_001756"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFS4976531.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10707..11342)
                     /locus_tag="ACG8OA_001757"
     CDS             complement(10707..11342)
                     /locus_tag="ACG8OA_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFS4976532.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11408..12811)
                     /locus_tag="ACG8OA_001758"
     CDS             complement(11408..12811)
                     /locus_tag="ACG8OA_001758"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFS4976533.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12798..13829)
                     /locus_tag="ACG8OA_001759"
     CDS             complement(12798..13829)
                     /locus_tag="ACG8OA_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4976534.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13860..14081
                     /locus_tag="ACG8OA_001760"
     CDS             13860..14081
                     /locus_tag="ACG8OA_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFS4976535.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14246..14983)
                     /locus_tag="ACG8OA_001761"
     CDS             complement(14246..14983)
                     /locus_tag="ACG8OA_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFS4976536.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15136..16476)
                     /locus_tag="ACG8OA_001762"
     CDS             complement(15136..16476)
                     /locus_tag="ACG8OA_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS4976537.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16713..18236)
                     /locus_tag="ACG8OA_001763"
     CDS             complement(16713..18236)
                     /locus_tag="ACG8OA_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFS4976538.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         5178 a         3910 c         3103 g         6159 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccc ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaaatt aaataaaata
     6481 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
     6541 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
     6601 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
     6661 tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg
     6721 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
     6781 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
     6841 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
     6901 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
     6961 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
     7021 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
     7081 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
     7141 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
     7201 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
     7261 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
     7321 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
     7381 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
     7441 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
     7501 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
     7561 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
     7621 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
     7681 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
     7741 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
     7801 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
     7861 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
     7921 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
     7981 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
     8041 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
     8101 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
     8161 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
     8221 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
     8281 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
     8341 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
     8401 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
     8461 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
     8521 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
     8581 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
     8641 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
     8701 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
     8761 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
     8821 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
     8881 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
     8941 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
     9001 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
     9061 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
     9121 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
     9181 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
     9241 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
     9301 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
     9361 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
     9421 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
     9481 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
     9541 acggcgtttt gaaagtaggt agctgccgtt ttctttatct ttgccgatcg tactgataac
     9601 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
     9661 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
     9721 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
     9781 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
     9841 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
     9901 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
     9961 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
    10021 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
    10081 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
    10141 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
    10201 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
    10261 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
    10321 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
    10381 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
    10441 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
    10501 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
    10561 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
    10621 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
    10681 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
    10741 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
    10801 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
    10861 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
    10921 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
    10981 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
    11041 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
    11101 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
    11161 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
    11221 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
    11281 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
    11341 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
    11401 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt tcccaatgaa
    11461 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
    11521 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
    11581 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
    11641 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
    11701 gctccatcca ttttttttgc agctcatcaa agggagcttg ctttgtggca tattccaaat
    11761 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
    11821 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
    11881 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
    11941 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
    12001 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
    12061 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
    12121 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
    12181 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
    12241 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
    12301 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
    12361 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
    12421 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
    12481 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
    12541 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
    12601 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
    12661 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
    12721 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
    12781 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
    12841 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
    12901 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
    12961 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
    13021 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
    13081 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
    13141 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
    13201 ctttgttgca gataaacctc tgactcatca ttgttttttg ccagctgata aggaagcagc
    13261 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
    13321 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat
    13381 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
    13441 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
    13501 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
    13561 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
    13621 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
    13681 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
    13741 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
    13801 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
    13861 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
    13921 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
    13981 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
    14041 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
    14101 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
    14161 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
    14221 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
    14281 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
    14341 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
    14401 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
    14461 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
    14521 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
    14581 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
    14641 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
    14701 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
    14761 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
    14821 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
    14881 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
    14941 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
    15001 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
    15061 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    15121 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    15181 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    15241 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    15301 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    15361 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    15421 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    15481 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    15541 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    15601 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
    15661 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    15721 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    15781 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    15841 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    15901 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    15961 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    16021 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    16081 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    16141 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    16201 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    16261 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    16321 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    16381 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    16441 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    16501 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    16561 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    16621 atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    16681 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    16741 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    16801 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    16861 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    16921 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    16981 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    17041 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    17101 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    17161 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    17221 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    17281 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    17341 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    17401 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    17461 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    17521 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    17581 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    17641 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    17701 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    17761 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    17821 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    17881 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    17941 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    18001 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    18061 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    18121 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    18181 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    18241 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    18301 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa
//
LOCUS       DAZULO010000053        18167 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000053 DAZULO010000000
VERSION     DAZULO010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.054"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..152
                     /locus_tag="ACG8OA_001764"
     CDS             <1..152
                     /locus_tag="ACG8OA_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4976539.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="ACG8OA_001765"
     CDS             complement(230..763)
                     /locus_tag="ACG8OA_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFS4976540.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="ACG8OA_001766"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="ACG8OA_001766"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFS4976541.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="ACG8OA_001767"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="ACG8OA_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFS4976542.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="ACG8OA_001768"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="ACG8OA_001768"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS4976543.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="ACG8OA_001769"
     CDS             complement(4488..5066)
                     /locus_tag="ACG8OA_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFS4976544.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="ACG8OA_001770"
     CDS             5388..5858
                     /locus_tag="ACG8OA_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFS4976545.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="ACG8OA_001771"
     CDS             complement(5966..7192)
                     /locus_tag="ACG8OA_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS4976546.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="ACG8OA_001772"
     CDS             complement(7946..8146)
                     /locus_tag="ACG8OA_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4976547.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="ACG8OA_001773"
     CDS             complement(8280..8888)
                     /locus_tag="ACG8OA_001773"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFS4976548.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="ACG8OA_001774"
     CDS             complement(8973..9443)
                     /locus_tag="ACG8OA_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFS4976549.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="ACG8OA_001775"
     CDS             complement(9491..10405)
                     /locus_tag="ACG8OA_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS4976550.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="ACG8OA_001776"
     CDS             10551..11354
                     /locus_tag="ACG8OA_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFS4976551.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="ACG8OA_001777"
     CDS             11532..12479
                     /locus_tag="ACG8OA_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFS4976552.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="ACG8OA_001778"
     CDS             complement(12547..12840)
                     /locus_tag="ACG8OA_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFS4976553.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="ACG8OA_001779"
     CDS             complement(12868..13533)
                     /locus_tag="ACG8OA_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFS4976554.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="ACG8OA_001780"
     CDS             complement(13673..14305)
                     /locus_tag="ACG8OA_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4976555.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /gene="liaS"
                     /locus_tag="ACG8OA_001781"
     CDS             complement(14312..15379)
                     /gene="liaS"
                     /locus_tag="ACG8OA_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFS4976556.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /gene="liaF"
                     /locus_tag="ACG8OA_001782"
     CDS             complement(15376..16107)
                     /gene="liaF"
                     /locus_tag="ACG8OA_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFS4976557.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="ACG8OA_001783"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="ACG8OA_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFS4976558.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="ACG8OA_001784"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="ACG8OA_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFS4976559.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         5391 a         3684 c         2906 g         6186 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaag
//
LOCUS       DAZULO010000054        17838 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000054 DAZULO010000000
VERSION     DAZULO010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.055"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..934
                     /locus_tag="ACG8OA_001785"
     CDS             122..934
                     /locus_tag="ACG8OA_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFS4976560.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            931..1731
                     /locus_tag="ACG8OA_001786"
     CDS             931..1731
                     /locus_tag="ACG8OA_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFS4976561.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1892..3160
                     /gene="rseP"
                     /locus_tag="ACG8OA_001787"
     CDS             1892..3160
                     /gene="rseP"
                     /locus_tag="ACG8OA_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFS4976562.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3228..4937
                     /locus_tag="ACG8OA_001788"
     CDS             3228..4937
                     /locus_tag="ACG8OA_001788"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFS4976563.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5149..9507
                     /locus_tag="ACG8OA_001789"
     CDS             5149..9507
                     /locus_tag="ACG8OA_001789"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFS4976564.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9652..10125
                     /gene="rimP"
                     /locus_tag="ACG8OA_001790"
     CDS             9652..10125
                     /gene="rimP"
                     /locus_tag="ACG8OA_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFS4976565.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10148..11323
                     /gene="nusA"
                     /locus_tag="ACG8OA_001791"
     CDS             10148..11323
                     /gene="nusA"
                     /locus_tag="ACG8OA_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFS4976566.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11345..11638
                     /locus_tag="ACG8OA_001792"
     CDS             11345..11638
                     /locus_tag="ACG8OA_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFS4976567.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11635..11946
                     /locus_tag="ACG8OA_001793"
     CDS             11635..11946
                     /locus_tag="ACG8OA_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFS4976568.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11959..14265
                     /gene="infB"
                     /locus_tag="ACG8OA_001794"
     CDS             11959..14265
                     /gene="infB"
                     /locus_tag="ACG8OA_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFS4976569.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14292..14639
                     /gene="rbfA"
                     /locus_tag="ACG8OA_001795"
     CDS             14292..14639
                     /gene="rbfA"
                     /locus_tag="ACG8OA_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFS4976570.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14796..15252)
                     /locus_tag="ACG8OA_001796"
                     /pseudo
     CDS             complement(14796..15252)
                     /locus_tag="ACG8OA_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            15417..17779
                     /locus_tag="ACG8OA_001797"
                     /pseudo
     CDS             15417..17779
                     /locus_tag="ACG8OA_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
BASE COUNT         6099 a         3161 c         3914 g         4664 t
ORIGIN      
        1 taaaaaaaga aaaaattatt cagtttttaa ataaaaaaac aatgatttca taggaaaaac
       61 agttagatat tgctataata cttaagatag tgggaatctt ttaagtatag gaggaaaaag
      121 aatgttacgt ttttttccgc aaaaaaacaa atatattcaa gaaaaaagcg aatttcaatt
      181 taataccgat ggaccaattc ctcagcacat tgccatcatc atggacggca atggtcgttg
      241 ggcgcaaaac cgtcgtttgc cacgagttgc tggccataaa gaaggcatgg aaacagtaaa
      301 gaaagtcaca aaaaaagcat caaggcttgg agtgaaagtg ttgacgcttt atgccttttc
      361 gactgaaaac tggaagcgac caaaagatga agtaagtttt ctcatgcaac tacctgtcga
      421 cttctttgat acgttcgtac cagaattgat taaagaaaat gtcaaagttc atgtcatggg
      481 ttatgaaaac gtattgccag aacatacgca agatgctgta agacgtgcaa ttgagcaaac
      541 aaaaaacaat actggaatgg tattgaattt tgctttgaat tatggaagca gagcagagat
      601 cgtgactgct gtccaagaaa ttgccgaaga agtagcaaaa ggagaaatac acgcagaaga
      661 aatcgatgat gaattgattg ctaagcattt aatgacaggt ttcttgccta aagagttaca
      721 agatcctgag ctgatgatcc gtacaagtgg agaagagcga attagtaatt ttctgttatg
      781 gcagatcgca tatagtgagc tgtattttac aaaagcgctt tggcctgatt ttgatggtgc
      841 gcatcttgaa gaagcaatcg cctcttatca aaatcgagac cgtcgattcg gcggtgtaaa
      901 aaaaacagaa gatgagaaag gggatcagtc atgaaacaac gtgtggtcac agcagccgtg
      961 gcgctagtct tgtttattcc gattatatgg atcggaggca tagcggttga gcttacagca
     1021 gcaatcttag cagtaatagg agtttacgag ctgtttcgga tgaaaggatt gacactctta
     1081 agttttgaag gagttttatc cgccgttggt gcagtgttac ttgttttgcc aaaagaaaga
     1141 tggtttttct ttcttccaga gaatgtaagt acatttatgt tattttatct tatagttatg
     1201 attcttttag gaacatcagt tatttctaaa aatacgtata cgattgatga agcaggtttt
     1261 cctgtgcttg tcagcttgta tgtgggaata ggtttccaga attttgtcaa tgcacgaaca
     1321 gaaagcttgg ctgttttgat atttggtcta tttattgttt gggcaactga tatcggagca
     1381 tatatggttg gtcgacgtta tggtcagcat aagttaagtc ctgatatttc gcccaacaaa
     1441 acgattgaag gtgcgctcgg tggtattggt agtgctgttg tagtcgcatt tctttattta
     1501 ttgctatacc cagcaaaaga actatttaat catggtatgt ttgtcatgct tctgatgacg
     1561 attatttttt cagcagtcgg acaatttggt gatttggtcg aatctgcaat caaacgtcat
     1621 tatgatgtga aagactcggg aaatatctta ccaggccatg gtggaatctt agaccgtttc
     1681 gacagtttgc tgtttgtatt tcctatcatg catttatttg gcattattta gtaagtatac
     1741 ctgcgtttct ttgtgaattt cataaaagac gcaggttttt atttttcatt atattgatac
     1801 tattggtgtc tttggtatac tatggtaaaa ttatagtgtt ttatcgttgt tttataaaaa
     1861 acacaaaaaa caagtaaagg agaaaaaagc tatgaaaacg attctgacat ttatcatcgt
     1921 ttttggtata ttagtgattg ttcatgagtt tggtcatttc ttctttgcga aacgatcagg
     1981 aatcctcgtt cgcgaatttg ctatcggtat gggtccgaaa atttatgggc atcaagcaaa
     2041 agacgggaca acatatacgc ttcgcctatt acctattggc ggttatgttc gaatggcagg
     2101 caatggggat gatgaaacag aaatggcacc aggaatgcct ctttctcttt tactaaattc
     2161 tgacggcatc gtagagaaga taaatttaag taagaaaatc caattgacaa atgctatccc
     2221 gatggaattg agccgttatg atttagaaga tgagctgact atcactggtt atgtgaatgg
     2281 agacgaaaca gaagtagtaa cctaccctgt tgatcacgat gcgacgatta ttgaaaacga
     2341 cggaacagaa atcagaattg cgccaaaaga cgtacagttc caatcggcta aattgtggca
     2401 gcgtatgctg accaactttg ctggacccat gaataacttt attttggcaa tcctgttatt
     2461 catcattctg gcatttatgc aaggtggtgt tcaagtgacg aacactaatc gcgtaggaga
     2521 gatcatgcct aatggagctg cggcagaagc aggcttaaaa gaaaatgatg aggtagtcag
     2581 tgtggacgga aaagaaatcc attcatggaa tgatctgaca acagtcatta cgaaaaatcc
     2641 ggataaaaca ttagacttta agatcgaacg agaaggtcag gtgcaatctg tcgacgtgac
     2701 accgaaatct gtagagtcaa atggcgaaaa agttggccaa cttggtataa aagccccaat
     2761 gaatacaggt tttatggata agatcattgg agggacgcgt caagctttca gcggttcatt
     2821 ggaaattttc aaagccttag gatcactatt tacaggtttc agtttagata aattaggcgg
     2881 acctgtcatg atgtaccaat tgtcttcaga agcagcaaat caaggtgtaa caactgttat
     2941 tagtttgatg gcgcttcttt caatgaatct cggaatcgtc aatctgcttc cgattcctgc
     3001 cttagatggc gggaaattag tcttgaatat ttttgaaggg attcgcggga aaccattaag
     3061 tcaagaaaaa gaaggaatat tgacattagc tggctttgga tttttaatgt tgttgatggt
     3121 gctagttaca tggaacgata ttcaacgatt ctttttctag aaaaagttga caaatagggt
     3181 ataataacaa acgataacta ttttgaggaa ataaaggagt atatagaatg agacaatcaa
     3241 aaatgttgat tccaacatta cgcgaagttc caaatgatgc agaagtatta agccatcaaa
     3301 tcttgctaag agctggatat atccgccaag tatcagcggg tatttattca tatctgccat
     3361 tagcaaatcg tgtattggaa aaactgaaac gcattatgcg ggaagaattt gaaaaaatcg
     3421 gagcggtaga aatgctgatg cctgccatct tgccagcaga actttgggaa gaatccgggc
     3481 gttacgaaac atatggacca aacttgtatc gtttcaaaga ccgcaatgat cgaaaaatga
     3541 tccttggacc aactcacgaa gaaactttta ctgatttgat gcgtaatgaa atcaattcat
     3601 ataagaaatt acctttaaat ctttatcaaa tccaacctaa atatcgtgac gaaaaacgac
     3661 ctcgcttcgg tttgcttcgc ggaagagagt ttatcatgaa agatgcgtat tctttccatg
     3721 caacggaaga aagtttggac gagacatata aagattacga aaaagcctac accgaagtat
     3781 tcaagcgttg tggcttagaa tttcgctcga ttatcggaga cggtggcgca atgggcggga
     3841 aagactcaaa agaattcatg gcgatctcag aaatcgggga ggatacgatc tgttattcga
     3901 ctgaaagtga ttatgctgca aacttggaga tggctacaag ctactatgta tcaaaaaaat
     3961 cgcatgaaac acagcttgaa ttggagaaag tagctacacc taacgtaaaa tcaattgacg
     4021 aagtagcaga tttcttttct gttgaacctc aaaaaatcat caagtctgta ttgttcattg
     4081 cggatgaaca acctgtcctt gttttggtac gcggagacca tgaagtcaat gacgtaaaac
     4141 tgaaaaactt tttatctgct gactttttag aagaagcaac agaagaagaa gcacaaaaat
     4201 atctaggtgc tgagtttggt tctgtcggac cagtcggtgt ttcagatgac gtaaaagtat
     4261 atgcagaccg ttatgtccaa gatttagcga atgccattac tggagcgaac gaaacaggat
     4321 tccactataa aaatgtgaat ccagaacgcg atttcactgt actttcttat gaagatcttc
     4381 gttttgtaca agaaggagat ccttcaccag acaacaatgg tgttcttgcc tttactcgag
     4441 gaatcgaaat cggacatatc ttcaaaatcg gcactcgtta cagtgaatct atgggagcaa
     4501 ctgtactaga cgaaaatgga cgagaaaaat ttgtgattat gggctgttat ggtatcggtg
     4561 taagtcgttt attatcagca atcgtagaac aacattcaga tgaacaagga atcaactggc
     4621 caaaaggaat tgcgccattt gatttgcata tcgtccaaat gaatctaaaa gacgaatacc
     4681 aaacaaacct gacgaatgaa ctagaagaag cgatgacaaa agctggctat gaagtactgg
     4741 ttgatgacag aaatgaacgc gcaggagtga aattcgcgga ttctgatttg attggctgtc
     4801 caatcagaat cactgtcggt aaaaaagcag tggatggaat cgtagaagtg aaaatcaaga
     4861 aaactggcga aatggttgaa gtacgtaaag aagaattggt gaatacttta ccaatcctct
     4921 tgaaccaagg cgaataataa gcgttaaaac acagaaaata aaaatagctg aatcagctgt
     4981 ttttgacaat ctgatgattc gcttttaaaa ttggaactgc gatttgaatc gcagttccaa
     5041 ttttttaatg taaaactcag aaaaaacgag tatactaaaa ggtactgaaa aagaagagga
     5101 taagatgcga tcaattgcat tgtttctcat cagaaagggc gtatatcttt gtcagaaaaa
     5161 cgagatttat ttgaaaaact gttggaacaa atccaactag aggaagcaga aaaaaatcat
     5221 ccactgctta ctgctggtga aatggatagt gtagtggtcc atcgtaaatc gcgtttatgg
     5281 gaatttacgc tgcatttttc caaaattttg cctattatgc tatatcggtc attgactcag
     5341 catctgacta tcgcttttaa agatattgca caagtcaaac tgaatatctt agcagatgac
     5401 cagacattcg atgaacagct cttgcaagat tactgggccc aagctttgga aaatcagcaa
     5461 tgcgatacac cactagcaca gcaagtattg aaaacgcagg tacctgtgat aaaagatcgt
     5521 aaagtcatct tgccaatcga cagtacagga gctatttctt atttgaagca acaatacttg
     5581 ccattagtag aagagttgtt cgtcagttac ggattcccaa aatttcgtat cgaaccagaa
     5641 gtggatgagc agcaagcgga acgagtattg aaactttttg aagaacgcaa acaagaacag
     5701 gcagaagcct ttatgaagca agcagctgaa agtctagttg tccacgaaca aaagaaaaag
     5761 gaaagaaaag agcaatcgcc agctcttgaa ggacccatcc acatccagct aggacgaaat
     5821 attccagccg atgaaccaac gacaccaatg atcagtatcg ttgaagaaga acgaagagtc
     5881 acacttgagg ggtacgtatt cgacaaagaa gtcagagaac tacgttcaaa acgcaagatc
     5941 ttgactttga aaatcacaga ttatacttca tcatttatcg tcaaaaaatt ctcgaacggc
     6001 gaaaaagatg aacaagtatt tgatgcgatc agtgtcggta gctggttaaa ggtacgagga
     6061 agtattcaag aagatacgtt tgtccgtgat ttggtcatga atgcgcaaga tatcatagaa
     6121 gtcaaacata cacctcgcaa agattatgct cctgaagggg aaaaacgagt agagcttcat
     6181 gttcatagca atatgagtac gatggatgca acaaacagca tctctgatct tgtggcacaa
     6241 gcagggaaat ggggacaccg agctatcgcc attacagacc acggtggtgc gcaagccttt
     6301 ccggaagcac atagtgctgg aaaaaaagca ggtgtgaaaa tcttatacgg cgtggaagcg
     6361 aatgtcgtag atgatggtgt cccaattgct tataatgatg cacatgaagc attgagtgaa
     6421 gccacatatg tcgtatttga cgtggaaacg acaggattgt ctgcagtata tgacacgatc
     6481 atcgagctag ccgcagtcaa aatgtataaa ggaaatgtga tcgaaagctt tgatgaattt
     6541 atcgatccag gacatccttt gtcacgaaca acggtagatc tgactggtat cacggatgga
     6601 atggttcgag gatcaaagtc ggaagaagaa gtattgcgga tgttccttga attttcaaaa
     6661 gatacaattt tggtagccca caacgcggct tttgatatgg ggttcttgaa tactagctac
     6721 gcaaggtatg gtattcctga agcggccaat ccagtcattg atacgttgga attagctaga
     6781 tatctgtatc ctcaattcaa acgtttcgga ttaggtgtgt tatctaaaaa atttggtgtc
     6841 agcttggaac agcatcaccg agcaatctat gatgctgaag caactggtca cttagcatgg
     6901 atctttgtga aagaggcaat ggacaatcat aacatgcttt atcatgatca gctaaatgag
     6961 catatcggag aaggagattc ttacaaaagg gctcgacctt tccatgtaac gattttagca
     7021 aaaaatcagg caggactgaa agatttgttc aaactgattt caatgtcgaa tgtcgaatac
     7081 ttcgaacgcg tcccacgaat accacgctcc caattgaaaa agatgcgtga aaatctattg
     7141 atcggctcag cttgtgataa gggagaaata ttcgaagcca tgatgcaaaa aggggtagaa
     7201 gaggcaagaa accgagcaaa attctacgat tatattgaag ttatgccaaa agcggtctat
     7261 gcgcctttga tcgagcaaga attggtaaaa aatgaacatg atttggaaga aattatccaa
     7321 aatttggtag aaatcggaaa aagtttggat aaaatcgttg ttgcaacagg taatgtacat
     7381 tatctgaacg aagaagatgc catttaccga aaaattttga tcaattctat gggcggagcg
     7441 aatccgttga atcgtcatag tttgccggat gtccatttcc gaacaaccga tgagatgcta
     7501 actgcatttc actttttagg ggaagaaaca gcgaaagaga tcgtagtaga aaatacgaat
     7561 aaaatcgcag atatctgcga agaagtgatt ccagtcaaag atgaacttta tacaccgaag
     7621 attccaggtt cagaagatga aatatcagaa ttgagttaca caaaagcaaa acaaatgtac
     7681 ggagatccgt tacctgagat cattcaaaaa cgattaaaaa aagagttgaa ttctattaat
     7741 ggtaatggct tctcggttat ttatctgatc gcccaaaaac tagtgcataa aagtaatgaa
     7801 gatggctatc tagtaggttc acgtggctct gttggctcta gctttgttgc gacgatgaca
     7861 ggaatcactg aagttaatcc gttagcccct cattattatt gtccagagtg ccaatattcc
     7921 gagttttttg aagatggtac gtatggttct ggatttgaca tgccagaaaa acaatgtcca
     7981 aaatgtggtg cacgcttaaa caaagacggt catgatattc catttgagac atttttagga
     8041 ttccatggag acaaagtgcc cgatattgat ttgaacttct caggtgatta tcaagcagaa
     8101 gcccataact atacgaaagt attgtttggc gaagactatg tttaccgtgc cggaacgatt
     8161 ggtacagtcg ccgacaaaac agcatatggc tatgtaaaag gatacgaaag ggacaataat
     8221 ctgcagtttc gaagtgcaga agtcgacaga ttagcaaaag gcgctactgg agtcaaacgg
     8281 acaaccggcc agcatccagg ggggatcatc gttattcccg attatatgga tgtctatgat
     8341 tttacgccga tccaatatcc agcagacgat caaaattcgg aatggaaaac gactcatttt
     8401 gattttcact cgattcacga taatgtattg aaattagata ttctagggca tgatgaccca
     8461 actgttattc ggatgcttca agatttatca gggatcgatc cacaaacgat ccctacggat
     8521 gacccggaag tgatgcggat atttgccggt cctgaagtac ttggagtaag tcaggaacaa
     8581 atttattcca aaacagggac attaggtatt ccagaattcg gcactcgttt tgttcgaggg
     8641 atgctagaag aaacgcatcc tacaactttt gcagaactac tgcaaatttc cggactatct
     8701 catggtacag acgtatggtt aggaaatgcg gaagaattga ttcgacgagg agatgcgaca
     8761 ctggcggaag taatcggctg tcgtgatgat attatggttt atttgatcca tgctggatta
     8821 gatagcggga tggcatttaa aatcatggaa actgttcgta aaggacaatg gaacaagatc
     8881 cctgatgaat tgcgagaaac ctatctatcg gcgatgaaag aaaataatgt gcccgactgg
     8941 tatatcgatt cttgttcaaa aatcaaatat atgtttccga aagcccatgc tgcagcttac
     9001 gtattgatgg ctcttcgtgt cgcttatttc aaggtttatt tccctatttt gtattactgt
     9061 gcatactttt ctgtacgtgc agacgacttt gatctagtct ccatgtgcaa aggaaaagat
     9121 gctgtgaaac aagcaatgaa agaaatcacg gacaaaggat tagatgcttc cgtaaaagaa
     9181 aagaatcaac tgacagtgct ggagttagcc aatgaaatgc tggaacgagg attcaaattt
     9241 ggaatgattg atttgtataa atcggatgcc gttaactttg ttattgaagg tgatacgttg
     9301 atcgcaccat ttagagctgt cccaagtttg ggaaccaacg tggcaaaaca aatcgttgaa
     9361 gcaagaaaag acggtccatt tttatccaaa gaagatcttg ccacccgcgg taaagtttca
     9421 aaaacattga tcgaatatat gaacgacaat ggcgtattga aagatctacc agatgaaaat
     9481 caattatcgt tattcgatat gctataagca tctgttttcc tgtctgcttg taattttaag
     9541 cagagtatgg tatagttctt ataggtaatg atactaactg tgagtgagcg gatttttcgc
     9601 tcactctttt taatggaatt acgcacttaa ttttttaagg aggcgaaact tttgagtagc
     9661 gtcgttgaaa cagtcacaga aatggtgaca ccgattttag atgaacaaaa ttttgaactt
     9721 gtcgaagtcg aatttgtaaa agaaggaaaa agctggttct tacgagtatt tattgacaaa
     9781 gaaggcggta tcgatatcga agaatgcgcc tttgtcagtg agaaactgag tgaaaaactt
     9841 gatgcagcag atccagatcc gattcctcaa gcttatttcc tggaggtatc ctccccggga
     9901 gcagagcggc cattgaaaaa agaaagcgac tatgaaaagg cacttggtga ttatgtccat
     9961 gtctccttat accaaccagt agatggcgaa aaacaatatg aaggatttct tcaatcattt
    10021 gatgctgaac agcttacatt gaaaatacgc ataaaaacac gagaaaaaga attgactttt
    10081 gaccgaaaaa atattgcaaa agctcgctta gctatccaat tttaatcacg gagaggaatc
    10141 agaaaaaatg agtaaagaaa tgttaaatgc gttagacgct ttagaagctg aaaaagggat
    10201 ctcaaaagaa atcgtgatcg atgctttaga agcagcattg gtttctgcat acaaacgtca
    10261 ctatggccaa gcacaaaacg tcgaagtgga atttgatcag aaaaaaggaa agatccatgt
    10321 ctatgcggta aaagaagtaa cggaagaagt gatggattca cagttggaag tctcattgaa
    10381 agatgcactt ttaatcaatc cagcatatga gatcggtgat acgatccgtt ttgaagtaac
    10441 accaaaagat tttgggcgga tcgctgcgca aacggctaaa caagttattt tacagcgtgt
    10501 acgtgaagca gaaagaacga ttatttataa cgaattcagt gcttatgaaa aagacatcat
    10561 gcaaggaatc gtcgaacgcc aggataaacg ttatatttat gtcaatcttg gtaaaattga
    10621 ggcagttctt tctaaacaag atcaaatgcc aaatgaattt tatcaaccac atgatcgaat
    10681 caaagtttat gtttcccgag tagaaaatac gtctaaaggt ccgcaagtat ttgttagccg
    10741 cagccatcca gacctattgc gccgcttatt tgagcaagaa gtaccagaag tctatgatgg
    10801 aatcgttgaa attgtcagca tcgcccgtga agctggcgat cgttcaaaag tggcagttcg
    10861 ctcaaatgat ccaaatatcg atgcagtcgg tacatgtgtc gggccaaagg gacaacgagt
    10921 tcaagcaatc gtcaatgaat taaaaggcga aaacatggat atcgtcgaat ggaatgaaga
    10981 tccagctgta ttcattgcaa atgcattgaa tcctgcacaa gtcatggatg tgatcttcga
    11041 tgagacaaat ccaaaggcct gcacagttgt cgttccagac taccaattat cgctagcaat
    11101 cggtaaacgc ggacaaaacg cacgtttagc tgctaaattg actaaccata aaatcgatat
    11161 caaatcagaa tctgacatga cggaatttta tgaaaatcaa gaacaacaaa aagaaacaga
    11221 agaactgcat gatgaagcaa tcgttcagtc agatttaaca gacgatgaat atgaaacaat
    11281 tgcattcaac aatgaaacgg tcgaagaaaa accagaagct taactttagt aaggaggcaa
    11341 agagatgaaa caaaggaaaa ttcctttacg caaatccgtg gtttcaggtg aaatgaaacc
    11401 taaaaaagag atggtccgaa tcacccgttc aaaagaaggg gaagtatcga tcgatccaac
    11461 tggaaaaatg cctggacgag gagcgtatgt ctcacttgag ccggaagaag tacaacaagc
    11521 ttgggataaa aaaattctcg atcgagtact cgaaacaaca ttaacagatg agttttatca
    11581 agaacttctt gactatgtca ctcaccaaaa agcacgtaaa gagttatttg gaaaatgaat
    11641 acagttaatc ggcaaaaagc catgaatctc atcggtctag cgatgcgcgc gggaaaacta
    11701 atcacgggtg aagaactgac cattgctgaa atccgcaaac aaaaagcaaa attggtgttt
    11761 gtggcaaccg atgcaagcga gaatacaaga aaaaagataa aagataagag ttcatattat
    11821 gaagttcctt gctttgagct attttctgaa gcagaaatca cgcagatgat cggaaaacct
    11881 cgcaaggtat tcggaataat ggatgccgga tttgcgaaga agaccaagga gctaattgaa
    11941 ggttaggaag gtgattgcat gggcaacaaa agaatctatg aattagcaaa agaactcaaa
    12001 aaatctagta aagacgtcgt ggaaaaagca caacaacttg gcatcgatgt gaaaaaccat
    12061 atgggtgcga ttactagcaa tgatgagaag aaattgcaac aagcgttcaa aaacccaagc
    12121 acgaaacaac aagctgcaca acaagcaagc agaaaaccag ccaatcaaca gccgaacgat
    12181 caacgtaaaa aagaaacaac gaacaatcaa caaaaaaata aaaataaccg cagttatcaa
    12241 gatcgcggtc aagggagcgg tcaagtgaat caaggaaaaa aaccaacaac aaaccaaaat
    12301 aatacgggaa ataccagcag ccaaaatcgt caaggaacga gccaaggcgg caatcaaaac
    12361 cgtactaaca ataacaacaa accaaacaat aacaatcgcg gcaaatttaa taataataac
    12421 cgtaatcgtt ataataaaaa aggtaaaaaa ggcaaacagc aacaatcaac taaaccagct
    12481 gtaccaccac gcaaattccg tgaattgcca gatgtattag agtatactga aggtatgaac
    12541 gtagcagata ttgcgaaaaa aattcatcgt gagccagcag aaatcattaa aaaattattt
    12601 atgcttggtg tcatggtcaa tcaaaaccaa gcactggata aagatacgat tgaactgtta
    12661 gcgacggact atggcatgga accacaagaa aaagtccaag ttgacattgc tgatatcgat
    12721 aaattcttcg aggcagaaga agtcgatcca gataaattag tctcacgtcc gccagtcgta
    12781 acgattatgg gacacgtaga ccatgggaaa acaactttat tggatacatt acgccattca
    12841 cgtgtaacaa gcggcgaagc cggcggaatc acacagcata tcggtgccta ccagatcgat
    12901 attgatggta aaccaatcac tttcttggat acaccaggac atgcggcctt tacaagtatg
    12961 cgtgcacgtg gagcaagtat cacagatatc acgatcttag ttgttgctgc agatgatggc
    13021 gtcatgccac aaacagttga agcaatcaac catgccaaag ctgcaggcgt tccaatcatc
    13081 gtcgctgtaa ataaaattga taaaccaggt gcaaaccctc aacatgttat gcaagaatta
    13141 agtgagtatg agttaattcc tgaagcatgg ggtggagaaa cgatctttgt tgagatctca
    13201 gcgaaattcg gccaaaacat cgaagaattg ctggaaatga tcctgttagt cgcagaagta
    13261 gaagatttaa aagcagatcc aacacaacgc gcaatcggaa cagtgatcga agcacgtctt
    13321 gataaaggta aaggtcctgt gtcaacctta cttgtccaag aaggaacact tcatgttggt
    13381 gacccaatcg ttgtcggtaa tacttacgga cgtgtccgtg taatgacaaa cgatcttggc
    13441 cgtcgcgata aagaagcagg acctgcaacg ccagtagaaa tcactggctt gaatgatgta
    13501 cctcaagcag gtgaccgttt cgtagtcttc gaagacgaaa agacagcacg tgcagcgggt
    13561 gaagaacgcg cgaaacgtgc attattagaa aaccgtgctg ccacaagccg tgtgactttg
    13621 gataacttat ttgaaagcct gaaagaaggc gaattgaaag aagttaacgt aatcatcaaa
    13681 gcagatgtac aaggttcagc agaagcactg gctgcatcat tgaagaagat tgatgtggaa
    13741 ggtgtccgtg taaaaatcgt ccattcagct gtcggagcaa tcaatgaaag tgatgtaacc
    13801 ttagcagcag ccagcaacgc tattatcatc ggtttcaacg ttcgtcctac accgcaggca
    13861 aaagtgcaag ctgatactga agaagtcgat attcgtcttc accgtatcat ctataaagcc
    13921 atcgaagaaa tcgaaactgc gatgaaaggg atgctagacc ctgaatttga agaaaaaatc
    13981 accggtcaaa tgatcgttcg tgaaaccttc aaagtttcta aagttggtac gattgcaggt
    14041 gcctatgtaa cagaaggata tattcgtaga gatagcggtg tccgtgtaat ccgtgacgga
    14101 atcgtaatct atgaaggcca acttgcaagt ctgaaacgat tcaaagatga cgtgaaagaa
    14161 gtcaaaatgg gttatgaatg tggtgcaatg attgaaaaat tcaatgacat caaagtagat
    14221 gacgtaattg aaggatttat catggaagaa gtcaaaaatg actaaaaact gataaaagga
    14281 ggccaatcat tatggctaac taccgtgacc gccgagtcgg tcaagaaatc ttaaaagaag
    14341 tcaatgatat tttgcgcaaa aaggtccgtg accctcgtgt ggagaacgta acgatcacag
    14401 atgtgaaagt tacgggggac ttacagcaag caacgatcta ttacagcatt ttatctgact
    14461 tagcatcaga taagaaaaca gcacaagatg gattgaataa agcatcagga ttgattcgtc
    14521 gagagttagg gcacaatcta agtatttata agacacctga attgttcttt gaacttgatg
    14581 aatcagttgt ctatggcaac catatcgatg aattgcttcg cgacttgaac aaagactaat
    14641 caaaaactcc cgcaaatcct gtgaaatcag gggttgcggg atttttttgt tttcattaag
    14701 acgtattacc taagagttcc atgactttag tcttgcggcg tgtcagctaa ccaaaaaaga
    14761 gaaaaagaaa agtaaaaaat tcgactttct tttttctagt agtatataaa tttttgcata
    14821 tagataaaga tcaagatacc gataacggaa gctagattgt aatctatttt agtgacagtc
    14881 aggtatagcc aagtaactag aagatcagtg gaacctgcac cataaaagtt tgagtttgtc
    14941 ggtccgttga cagttaaaag aaaaatcacg ttgaagttat tgatatttcc gattacgccg
    15001 cttcgatttg gtcacgatcc aaattttgga taatccctgt agaaattagc atagtagctg
    15061 ggataccaat tcacatattg acaaagataa cagagacttt tacataaaac ggatcaatca
    15121 ggaaaggaat tgctcgatcc agccccagtt ttccagcaat gtgttaactg gcccagcgtc
    15181 attcagcaaa ttatgcatga tgaggagcga aatgaactgc ggaacagcca ttgtgataac
    15241 gacaagtttt ttggatcaat gtaaaaaacg tcagcgaaag gaaaaatttc gtggtttttt
    15301 atacctgttc atttggctag atataaaatc caaacaaatg aacagaagtg cttattaggt
    15361 ttcaattggg agtatctgcg ctagaataag agtaataatg aaaaggagtg ttatgtatgg
    15421 attattctag taaagaatac tttgataaaa tgacggcttg gtggcgtgct gctaattatc
    15481 tttctgttgg gcaactttat cttaaagata accccttact gagacgtaca ctgaaacccg
    15541 aagatgtcaa aaaacatcca atcggtcatt ggggaacgat tcctggccaa aattttattt
    15601 atgtccattt gaaccgagtc atcaataaat atgatttgaa catgttttat attgaagggc
    15661 caggacacgg cggacaagtc atggtatcga atgcttattt agatggaagt tacacagaga
    15721 tctatccaga agtaacggaa gatgagacag gaatgcaaaa attattcaaa cgattttcat
    15781 ttcctggagg cattgcttct catgcagcac cagaaacacc aggttctatc catgaaggcg
    15841 gagagcttgg atactctctt tcacatgcag tcggagctgt attagacaat cctgaagtaa
    15901 tctctgctgt cgtgattggt gatggtgaag cagaaaccgg tcctttagct ggaagctggt
    15961 tctcaaatgt ctttatcaat ccagtgatag atggagctgt tttaccgatc cttcatttaa
    16021 atggtgcaaa aatagccaac ccaacgatct tggcacgtaa atcagatgga gaattagcaa
    16081 attactttaa cggacttggc tgggaacctt tctttattga aggaaatgat tcagaaaaat
    16141 taaatcctgt tatggctgaa aaaatggatc aagcaatcga gaaaattaaa tccatccaaa
    16201 aagaagctcg cctgaagaca gcaacagatg tcgtgatgcc aaaatggcca gtattgatcg
    16261 tacgcacacc aaaaggatgg accggtccaa aagaatggga cggggaacca attgaaggaa
    16321 ccttccgagc acaccaagta ccgattcctg tagatcaaga acatatggat catgcagatg
    16381 ctttactccg ttggctgaaa tcctatgaac cagaaaaatt atttgatgca caaggacgta
    16441 ttttagaaga aatcagagaa atcgcgccta ctggtgacca aagaatggca aaaaatccta
    16501 tcaccaatgg tggaatcgat ccaagaccgc ttattatgcc agattggaaa aaatatactt
    16561 tgcaatttga aaaaccaggc tccgttaaag cagaagatat gaccgaactt ggtaaattcg
    16621 ttcgcgagat cattgagaaa aatccagaaa atttccgtat atttggacca gatgaaacaa
    16681 aatcaaaccg attgaatcaa gttttcaaaa cgacaaatcg ccaatggatg gaaaaaatcg
    16741 aaccagaaaa tgatgaatgg ctttcgccat ctggtcgcgt catcgacagt caattatcgg
    16801 aacaccaaga tgaagggttc ttagaagggt acgttctgac tggacggcat ggttttttgc
    16861 aagttacgaa tcattcttgc gagtggttga ctctatgttg actcagcact tcaaatggat
    16921 gcggaaatca catgaccttt cttggcgaaa tgattatcct tcgctaaatc tcattgcaag
    16981 ctcaacagtc ttccagcaag atcacaatgg atatagccac caagatccag gaatattgac
    17041 acatttggca gaaaagaagg cggagttcat ccgtgaatat ctgcctgcgg atgcaaatac
    17101 attgctcgca gtcatggata aagcattccg ctcgtcagaa aaaatcaatt tgatcatctc
    17161 ttccaaacat ccgcgtgctc aattttactc agcagaagaa gcagccgtct tagtgaatga
    17221 aggcttgaaa atcattgatt gggcttcgac agcaaaagag gaggaacctg aattggtcat
    17281 tgctgctgca ggaacggaat ctaacttaga agcattagca gctgtaactt tgctgctgga
    17341 agaattccca aaactaaaaa ttcggttcat caatgtcgta gatttattga aattacgtca
    17401 tccaagtcaa gatccgcgtg ggttgagcga tgaagaattt gatcaatatt ttacaaaaga
    17461 caaaccgatc ttatttgctt tccacggtta tgaaacattg gtccgcacga ttttctttga
    17521 tcgtcataac catcatttaa tgattcatgg ctacaaagaa aatggagata tcacgacacc
    17581 atttgacatg cgtgtagtaa atgaacttga tcgttatcat ttggcaaaag acgcagcttt
    17641 aaaaatcaaa ggaagtcaag cagaagattt tgcgaaaaaa atggatcaga aattacaaga
    17701 acatcaaaac tatattcgtg aaaatggtat tgatctgcca gaagtattgg attggaaatg
    17761 gaaaaatctt gaccaataaa atgataaaat cgatcccgca aaatagaatt ttttacattg
    17821 attaagaaac tgaaaaaa
//
LOCUS       DAZULO010000055        17526 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000055 DAZULO010000000
VERSION     DAZULO010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.056"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..1475
                     /locus_tag="ACG8OA_001798"
     CDS             138..1475
                     /locus_tag="ACG8OA_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFS4976571.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="ACG8OA_001799"
     CDS             1523..3652
                     /locus_tag="ACG8OA_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFS4976572.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="ACG8OA_001800"
     CDS             3627..4316
                     /locus_tag="ACG8OA_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976573.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="ACG8OA_001801"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="ACG8OA_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="ACG8OA_001802"
     CDS             complement(5546..5740)
                     /locus_tag="ACG8OA_001802"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976574.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="ACG8OA_001803"
     CDS             complement(5790..6230)
                     /locus_tag="ACG8OA_001803"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFS4976575.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="ACG8OA_001804"
     CDS             complement(6234..7079)
                     /locus_tag="ACG8OA_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFS4976576.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="ACG8OA_001805"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="ACG8OA_001805"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFS4976577.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="ACG8OA_001806"
     CDS             complement(8703..9650)
                     /locus_tag="ACG8OA_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4976578.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="ACG8OA_001807"
     CDS             complement(9873..10223)
                     /locus_tag="ACG8OA_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS4976579.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="ACG8OA_001808"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="ACG8OA_001808"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFS4976580.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="ACG8OA_001809"
     CDS             11646..11966
                     /locus_tag="ACG8OA_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS4976581.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="ACG8OA_001810"
     CDS             11988..12452
                     /locus_tag="ACG8OA_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4976582.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /gene="ytpR"
                     /locus_tag="ACG8OA_001811"
     CDS             12657..13262
                     /gene="ytpR"
                     /locus_tag="ACG8OA_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFS4976583.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="ACG8OA_001812"
     CDS             complement(13301..14590)
                     /locus_tag="ACG8OA_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFS4976584.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="ACG8OA_001813"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="ACG8OA_001813"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFS4976585.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15700..15825
                     /locus_tag="ACG8OA_001814"
     CDS             15700..15825
                     /locus_tag="ACG8OA_001814"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976586.1"
                     /translation="MIDEKKCLKNLLEDMDDIEENYLNGLGKQIYFRYLSYLKMK"
     gene            15822..16250
                     /locus_tag="ACG8OA_001815"
     CDS             15822..16250
                     /locus_tag="ACG8OA_001815"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFS4976587.1"
                     /translation="MRHSNFLAFLFAPNETHNLSDEFFKMFLKRYIDSNDDTKAAINY
                     FDALLNSYEDLIVYRENKNIDILLVSEKNKIVVCIENKILSSESGGQLNKYQIYIERN
                     YSNYKKIFVFLTPDGNEPTNPSWGIITYKNIVEESVKYFV"
     gene            16321..17490
                     /locus_tag="ACG8OA_001816"
     CDS             16321..17490
                     /locus_tag="ACG8OA_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFS4976588.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT         5612 a         3163 c         3520 g         5231 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata
    15661 cgtaaaattt tagttagaat aatggcaagg agaaaaaaca tgatagatga aaaaaagtgt
    15721 ttaaaaaatc tattagagga tatggatgat attgaagaaa actatctaaa tggactggga
    15781 aaacaaatat atttcagata cttaagctat ctaaaaatga aataagacat agtaattttt
    15841 tagctttttt gtttgctcca aatgaaaccc acaatttatc tgatgagttt tttaaaatgt
    15901 ttttgaaaag gtatatagat tctaatgatg ataccaaagc tgctatcaat tattttgatg
    15961 ctttacttaa tagctatgaa gatttaattg tttacagaga aaataagaat attgatattt
    16021 tattggtatc ggaaaaaaat aaaatagtgg tgtgcatcga aaataaaatt ctgtcttcgg
    16081 agtctggagg gcaacttaat aaataccaaa tatatataga acgcaattat tctaactata
    16141 aaaaaatttt tgttttttta actccagatg gaaatgaacc cacaaatcct agttggggca
    16201 taattactta caagaatatc gttgaagaga gtgtaaaata ttttgtgtaa atagaaaaaa
    16261 ggaagtccct tctgtagaat agagttacca caacacattc acagaaaaga ggacttccat
    16321 atgaacgatt ttactacaga aattatggaa atactaatca ataaaggtga tttggatgat
    16381 ttgtttcgtc gtcacctaga attggctatt aacgctttat tacaagctga attaacggta
    16441 tttcttgact atgaaaagta tgatagagct ggatttaatt caggtaattc ccgtaatggg
    16501 aattattcgc gctcttttaa aacagagtac ggtgaattga atttagtaat acctagagat
    16561 aggaatggag acttttctca acaaacttta cctgcttata aaagaaccaa tgactcttta
    16621 gaaaccacta ttattcagct agttcaaaaa ggaattacta tgtccgaaat ctctgatcta
    16681 atcgaaaaaa tgtatggtca ttattacaaa ccacaaacta tttcaaatat gagtaaaatt
    16741 gtgtctgaag atgtttcggc atttaaagaa agaactttag aagctaaata ctcagtcatt
    16801 tttatggatg ctactcatat tcccttaaaa agacaaaccg tatcaaaaga agctgtctat
    16861 atagtaattg gtatcaggtt ggatggaact aaagaggttc taggatttac tatagctcca
    16921 actgaatctg cttatgtttg gaaagagatt cttcaagatc taaaagaccg tggtttagaa
    16981 gaggttttgt tagtcgtaac agatggttta agtggtattg acgatagtat tcatagtgtt
    17041 tatccaaatg ctcaatttca acaatgttgt gtccatatat ctagaaatat cgctcataag
    17101 gttcgtgtta gtgatcgaca agaaatctgt agcgatttca aattagttta ccaagcaagt
    17161 tcaaaagaag aagcaaataa tcaaataagg tttatgatag ataaatggaa aaagcagtat
    17221 ccacgagtgg ttaaattgct catgaatcct gctatattaa ccttttataa tttccctccg
    17281 tcaatcagaa gaactattta ttcaactaat ttgattgaag gttttaacaa acaattaaag
    17341 aaatatacca aaagaaaaga gcaatttcct aatgaagaat ctctggagag atttctagta
    17401 tctcaattca acaactataa tcaaaagttt ctatgccgag ttcataaagg ttttaaagaa
    17461 atacatgata cattagaatc aatgttttaa cttgatcaac agaatgggct tttccattta
    17521 cacaaa
//
LOCUS       DAZULO010000056        17348 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000056 DAZULO010000000
VERSION     DAZULO010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.057"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..273
                     /locus_tag="ACG8OA_001817"
     CDS             13..273
                     /locus_tag="ACG8OA_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976589.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(635..874)
                     /locus_tag="ACG8OA_001818"
     CDS             complement(635..874)
                     /locus_tag="ACG8OA_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976590.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1160..1474
                     /locus_tag="ACG8OA_001819"
     CDS             1160..1474
                     /locus_tag="ACG8OA_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976591.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(1908..2274)
                     /gene="ssrA"
                     /locus_tag="ACG8OA_001820"
     tmRNA           complement(1908..2274)
                     /gene="ssrA"
                     /locus_tag="ACG8OA_001820"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2445..3218)
                     /locus_tag="ACG8OA_001821"
     CDS             complement(2445..3218)
                     /locus_tag="ACG8OA_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4976592.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            3362..3712
                     /locus_tag="ACG8OA_001822"
     CDS             3362..3712
                     /locus_tag="ACG8OA_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFS4976593.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            3693..4409
                     /locus_tag="ACG8OA_001823"
     CDS             3693..4409
                     /locus_tag="ACG8OA_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFS4976594.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            4409..5857
                     /locus_tag="ACG8OA_001824"
     CDS             4409..5857
                     /locus_tag="ACG8OA_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4976595.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(5927..6436)
                     /locus_tag="ACG8OA_001825"
     CDS             complement(5927..6436)
                     /locus_tag="ACG8OA_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976596.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(6426..7151)
                     /locus_tag="ACG8OA_001826"
     CDS             complement(6426..7151)
                     /locus_tag="ACG8OA_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976597.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(7374..9494)
                     /locus_tag="ACG8OA_001827"
     CDS             complement(7374..9494)
                     /locus_tag="ACG8OA_001827"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4976598.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            9724..10902
                     /locus_tag="ACG8OA_001828"
     CDS             9724..10902
                     /locus_tag="ACG8OA_001828"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFS4976599.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            10918..11676
                     /gene="aroD"
                     /locus_tag="ACG8OA_001829"
     CDS             10918..11676
                     /gene="aroD"
                     /locus_tag="ACG8OA_001829"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFS4976600.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            11699..12184
                     /gene="ybaK"
                     /locus_tag="ACG8OA_001830"
     CDS             11699..12184
                     /gene="ybaK"
                     /locus_tag="ACG8OA_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFS4976601.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(12255..13508)
                     /locus_tag="ACG8OA_001831"
     CDS             complement(12255..13508)
                     /locus_tag="ACG8OA_001831"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4976602.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            13626..14975
                     /gene="gdhA"
                     /locus_tag="ACG8OA_001832"
     CDS             13626..14975
                     /gene="gdhA"
                     /locus_tag="ACG8OA_001832"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFS4976603.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            15087..16433
                     /locus_tag="ACG8OA_001833"
     CDS             15087..16433
                     /locus_tag="ACG8OA_001833"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFS4976604.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(16741..17127)
                     /locus_tag="ACG8OA_001834"
     CDS             complement(16741..17127)
                     /locus_tag="ACG8OA_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4976605.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(17176..>17348)
                     /locus_tag="ACG8OA_001835"
     CDS             complement(17176..>17348)
                     /locus_tag="ACG8OA_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFS4976606.1"
                     /translation="VETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQR
                     RFEGTLRYSLGY"
BASE COUNT         5695 a         3033 c         3449 g         5171 t
ORIGIN      
        1 gatggatgaa atatgaataa cgaaactatc attttaggaa aaaaatatat ttgccaacct
       61 attggcttga aacaaaaagt ggttggagaa gtaatcagta aattagaaaa ttgtgttgtg
      121 atctgcgtag acgaatatca tgacgtcgat cgcaatgaaa tattagaaaa atgcggaaaa
      181 gtagtggcaa aatatactca cgtttatgag ttggaaacaa cagaatatct atataactat
      241 agcaacaaat atgaacatac tttagtttca taaagattta aataaagtgc cttcaaaagc
      301 gagaagacca agcttttgaa ggcactttat ttagttggca gtgattgttc atgatttttg
      361 atttattcaa aaggttcgcc tagagaatct gtctctgtaa cgaggcctgg tggagaaact
      421 cgataaaaac ctatactgcc agaactgtcc tattttcgga tagacaaact agtagtttta
      481 aaaagatcat ccgatccaat catttcaaaa aatgcaaaac tgatatcagg atcatttagc
      541 tcaggcttgt tatcgtaaag catttgtaaa ggttgcattt agttaggagt aaacgcgaaa
      601 ctatcatcat tatttgctga agaagatgaa gaagctaatt cagagtttgt ttgaaataaa
      661 ggagacgtgc tgacagtttc tttaccctgt tttttgcaga ggtagaaact tttatatcgg
      721 aagtttctac cggagaattt ttctcatttg tagaaattgt tgaatcgata ctattcgaaa
      781 tttgcgtatc tgaaggtatg ttctttgatg cagaagtccc acaagcagta agtaacaata
      841 aatttgataa actgataaac agaattcttt tcattatgta cacactcctt atagtatcgt
      901 ttagcataca cctatgctca gtattaggca aaatcaatgc aaaactatca agtcttttat
      961 agtatctatt agtcttagta atacatatat ggagagggtg atatcccagt tcaaaccaga
     1021 tacattggtt accatctgta ttaacgatta tgcctctaaa cgcttttttt atgaaaaaat
     1081 ttataagtga aaacgtaggt atgatattat ctagaggtaa tttttttggg tatttaaata
     1141 gttgctagga ggatagcttt tggattttta cagtaatttt atcttaataa tagcaatatt
     1201 attacttcta aatatttggt tttttgataa gagtagaaat gcagggatag gttttagaac
     1261 caaacgaagt acgtcttctg aaaaaaagtg ggtatattca cagactattt tttacggagg
     1321 ggttatttca atcagtttac tttcttcaac actttattct tttaatgtta tagacgtttc
     1381 catgtcaaat tttatatcta ttattggaat ccttatttct gcaattatta cacaactact
     1441 tcttgtgttt gaagagaagt caaaaaataa ttaaagcaat tatgcctttt agtacaggaa
     1501 ttattacttc ttacaaatgt atagcgaatg tgaagcctgc tgttttaatt gttctagaaa
     1561 aacttttgag tgaacaatta ttttatgtta ggatgaatta atttctctag agtttataat
     1621 taaaaaactt atttgaagga acatgcttaa accataattg tgtttttcct tccatcagca
     1681 taaaagttca cctcaaaaag agtgtacgaa caaaagttct ttttgtaaac cttaaaatag
     1741 attttcctga atacgtattg gggatttcta aatgtttgaa tgtttttttg aatgcaaaat
     1801 caatgcaaaa aattttgtat ttctctactt ttctctcctt gaccaaaata aaaaaacgtt
     1861 gatttaacaa cgtttttcat acatgtttat ctgtctcaac catacatata tggagacggc
     1921 gggagtcgaa cccgcgtcca aacacatcgc cacttaaaaa tctacgttca tagtcaactc
     1981 atttaaggat tcactttaca gcttgccgag tgacaggcat tctgtatcgc tagtctgatg
     2041 ttctcttttt caacttacag acggaaagtt gaaacgtatc ccactaaagt taggaccctt
     2101 actcgagcac atgggcgatg ccgagaggat ctacgctaac tgtttttagg cagctaaagc
     2161 gtaagagttg ttttcgtttt tagcagttat atttaactgt aacgttttta cgtagacgta
     2221 acctacgaaa cgcaattcaa gctcgactgt gcctgtcgaa tccgtaacgt ccccgttagc
     2281 taacaagggt tacgtgtatt tttcatacaa aattagtata gcatatattg ggataaattc
     2341 aataaaaaag cgaatcatat cccattattt atataataac tgcccctttc ttaagaaaaa
     2401 ataagcgatt ttcttaagaa aagaggtagc ttcaaaaaaa gatcttaaat cagatcaaaa
     2461 tacttcaatg cgtggacaat tccgccttct gtatttttct ttgtgataaa ggtagaaagt
     2521 tcttttaatc cgtcataggc gttgcccata gcgattttgt tgtcgcaagc ttccaacaag
     2581 gcaaaatcat tcggaccatc gccaaatcca aaagtaggaa ctttttccag ctgaagttct
     2641 ttttgcagac gtttgacacc tgcccctttt gagattcctt tcttcactgt atcgatcgag
     2701 taaggaccat tccgataaaa agttagctca ggaaaatgtt cgtgatagta ttcgtcacct
     2761 gattgagata agatcagcaa catatttact gtgttttctt taaagcctaa aggatcaatt
     2821 tccggaacat ctgaatgaat gaagtcgtaa gcattgatca ttgtttggct atgaccggta
     2881 caccagattt tctgatcgtt atagtaagaa agttcatgac cgttcaactg tacatgatca
     2941 tacatttttt gacattcttc tgaagaaaat gtttgagaat agatttcttc gccgtctact
     3001 cgaatgaaag aaccattcat ggtgaccgca gaagtaatgc ccgcatcttc catgatagag
     3061 tggatttctg cttctgtacg tcctgtagcg atgacgggga gaacattgtt ttcttttaat
     3121 gcgttcatag cttttgccac ttcgggtgtg atttttgatt tcgcatctag taatgttcca
     3181 tcaagatcaa agaaagtgat tgcacgataa tttgacacaa ataaaactcc ttttttattg
     3241 gaataagttt atcttaacaa agaatgaaca aaagacaaac ctttcgtgaa gaattcctga
     3301 acatattggt tcagtcaaca atttagagta tactataaga agagaattta ggagggaaag
     3361 aatgattttt ttcagtattg gtatcttatt cttaataatt ggcagtcttt ttcggattct
     3421 gccctcaaaa ggaaatctgc ctttttatgg gtatcattcg cctcttgccg caaaaacaga
     3481 cgctcattgg cggctggctc aaaagacaag cggaaactgg ttttttctta tgggcttact
     3541 gatggcattg atcggctatt atttaaagac atctgggcat acgaattatt ttctgattga
     3601 aatgcttctg ctagtatttc caattatgcc tatttttatt atgacagaaa agaaattaca
     3661 gaaatatgac ctggaaactg gagggaacga taatgaatat tttaatgatt gaagataatc
     3721 aatctgtttc tgaaatgatg cagatgtttt tcttaaatga aggttgggaa gcaactttca
     3781 agtatgatgg aaaagaaggg ctcgatgcct ttttagaaaa cccacaacat tgggatatga
     3841 ttacactaga cctgaatttg cccacactgg acggtatgct agtaggaaga gagatccgca
     3901 aagtttcaag tactgtgccg attatcatgc tgacagctag agattctgaa agcgatcagg
     3961 tgatcggctt agagatgggg gcagatgatt atgtcacaaa acctttcagt cctctgacgc
     4021 ttattgctcg aatgaaagct ttgcatcgcc gtagtgagtt ggcagaagca agagaagaag
     4081 cagcagtttc agatgatcat tttgatatcg agactagtca tttcaagatg aatacgaaaa
     4141 cacgagaagc gtatctggac aaccggccaa ttgaaggact gacgcctaaa gagttcgatc
     4201 tattgtttac tttagcaaaa aaaccacgtc aagtattctc tcgggagcag ttgcttgaat
     4261 tagtctggga ttatcagtac tttggtgatg aacggacagt ggacgcccat atcaagaaat
     4321 tacgacaaaa aatcgaaaag gtcggaccac aagtcatcca aactgtttgg ggagtcggct
     4381 acaaattcga tgattctggt gttgcctaat gcgttatttg tatcagcaac tcttagcttt
     4441 ttgcggaatg gtcgcgttga tcttattgat cgttggagtt tcttttacac aactgacaaa
     4501 acaaacgatt gaagaaaaca attaccagca actgtttgga tatgctgaat cagttgaaaa
     4561 gacaacgcaa acctatgccg atacgctccc gcagtttagt caagatcagg cgtttcaaaa
     4621 tgcattgctg ctgacagagc aagtactgac agaacaaaat gtgaacttta tatttatcga
     4681 taaaaacgaa cgtgtcattt atccgactaa tacagcttta ctgaatttta ccttaacagc
     4741 tgatcaatgg aaaaatctga ggaatggacg tcaagaaaaa ttcacatcga ataaaaatat
     4801 tttaggacaa gcacaggcga cctcttacgc acttgtgcca tttaatttga atcatgaatt
     4861 ctatggagca cttgtagtta gtcaacctgc gcgaaatatc gataacagcg ttcgtccaat
     4921 tacactgaac ctgttcaaag gatttatttt ttccagtatc gttgcagtta ttgccagcta
     4981 tgcttttgca gcattccaag ttaagcgtat caatcggtta aggaatgcga cgaaagaagt
     5041 gacgaatggt aattttgatg tacagctccc cgtacatgac aaagatgaat ttgatgagtt
     5101 agcagatgat ttcaataaaa tgacgaattc attgaaagaa tctcaagcag aaattgaaga
     5161 acaagaaaat cgtcgaagac aatttatggc agatgcttct cacgagatga gaacacctct
     5221 tacgacaatc aacggacttc tggaagggtt agaatacaat gcaatccctg aaaatcagcg
     5281 agaaaacgcg attaaattga tgaaaaatga aacagagcgt ctgatccgtt tggtgaatga
     5341 aaacttggat tatgaaaaaa tccgtacgaa tcaaatcagt atggtcatta aaaaattcaa
     5401 tggaacagaa acattgaaaa atattgttgc acagttagaa gctaaagcag aagcagcagg
     5461 agatacattg acattgaaag ccgttgatga tattgatgtt tacgctgatt atgaccgatt
     5521 tgtccaaatc atggtcaata ttattcaaaa cgcgatacag tttactgaaa acggccaaat
     5581 cacgattact ttagaaaaag gctatcttga aacgatcatc acgattgaag atacgggaat
     5641 tggaatgtct gaacaacaaa tgaaaagtat ctgggatcgt tattataaag tagatccttc
     5701 aagaaaaaat acaaaatatg gtgaatcagg attaggttta ccaatcgtgc agcaacttgt
     5761 acgcttgcat aaaggaaaat tagaagtaga aagcgaacta ggaaaaggaa caaaattcgc
     5821 tgttcgtttg cctgacgtag aaatcaaaaa agagtaaaaa acaagcggta taaaagatca
     5881 tgaatcgatc ttctataccg cttgttttta ctataaaatg attgatttag cccgcgggct
     5941 ctaaacgatc aatataagga ctttgagcaa tagtagcatc atgagtaaag agtaagatac
     6001 cttgattttc ttcatgagct aaataagaaa gcaagtggag tagctcttgg atctccaata
     6061 cactaagttc atcaaatgta tcttcaataa caatattgtt tcgcttggaa agcaaggcat
     6121 gaagtaattg cagctttact ttttcaaaag acgtcaactc agctgccgca caattaagca
     6181 aggaaggaga gatatcaagt ttttcttgat aggccaaacg atcttgtttg atttcttttt
     6241 ccggtacatc caacaggaca tggtctttaa gattcagata aggaaacatc ggccaatttt
     6301 tttggatgat cgcaaaatta tccagttttg caacaagaac ctcttttttc aagtgctcca
     6361 atattttatg ctgtttttcg agaggacaca aaataacggt caagtgttca gcatgaatca
     6421 aatattcagg tttcattacg tccaggcctc ctactttgca aatgatggat aaaaaaagtg
     6481 aaagggaata gactaagaat gagacagcat ccactgagcc caataagagt ttgggcaaat
     6541 cccttaagta tatctgtgaa aaaaagatca ttttggaaat cgatggtaaa aaaagtctgt
     6601 tgatccatcg gaattgtaag tccgcttacc gaagtcaaga tatcacggat atctggcagt
     6661 tcagtagtct ttaagacaag aaaattgatc ctttgaagaa gcgcttcata aactgactgg
     6721 aagcttacga tccacaataa aaccaagata caacaaagaa agagaggaaa gaacagctgc
     6781 cagaaaataa aaaggaataa tttggtttta gaaagaccag tcaggcgcca attgatcaat
     6841 tcctgcttgt tttgcctgac tgaatagtaa aagaaaaaga taaaaaggaa ggcaaataga
     6901 aaaaataaac tgaatatgcc ttttatataa aatcggctag ctaattctaa agccggaaga
     6961 agctctgtag atgcagaagc gccaataaat gaaagccgat cttggacttg attggctaaa
     7021 gccgattgca aatggtaaag agtcaggcat gctatcaata aaaaagcaga aaagcttgaa
     7081 aataaacaat agagtattgt ctgctttttg taataaatta atcgagcagt agcatagcgg
     7141 attattttca tacttttcac ctctataaaa agtatagaaa atgaatatga ataaagtatg
     7201 aaattccttt agtgaaatca cgaattaaaa ccagaaaata ttttgtaaaa agcaagaggt
     7261 tgtgaagaag ctgccctatt ctattaccgt agatgcaagc ttttgaacac tgtttattcg
     7321 aaaagaggag cagcgacaat aattttgtcg ctgctccttt taaatttatt ttgtcattcg
     7381 gaagaacttg tggtcgttcc gtgaagttct tggtacgttt gcgttttata aagatcaaca
     7441 gttgattgtc cgttgtgttc actaaagaca ctagttgact tgtcaccaag agatttttca
     7501 gcttctttta atttctctaa gccattcttg tagtcataat cactggtatc aattgggtct
     7561 aatccactat tcgtatagaa ccgaagaaga tcaccattat tgatttggtc agaaacagct
     7621 aactgtttat caactttttc tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt
     7681 tcagtaatag cttcaccagt ttcgttacta tagatcgtac caccgtagta agtatatttt
     7741 ggtgtaacaa aatcaccatc acggaaagaa acaagctgtt catgatcttt cgagaataga
     7801 tcttgtccta actgaatata attttttgta tctacaccta gtaagtgaag taatgtaggc
     7861 aaggcatcaa cttgtccgcc gtaagtgtga ttgattccgc cctgtgtttg tccagggata
     7921 tggatcatat aagggacacg ctgcatttgt gcattgtcgt agtttgtcca agtagaactt
     7981 gtttttccaa ctaattcagc tagattttta tttcttgaat tcgagacacc gtaatggtca
     8041 ccgtaaagga cgatgactga attatcatac aagccgcttt ctttcaaata attgaagaat
     8101 tcttgtacgg cggtatctaa ataattggct gtcgcaaagt aaccgttgat cgtttcatca
     8161 tttgtattgg ctaacgggaa tcctgcttcg tcattggtga attttgaata aggataatgg
     8221 ttagatacag caatgaattt tgaataaaat ggttgttgca gacgttccaa gtattgtacg
     8281 gattgttgga aaaatggttt atcatgcaac ccgtattgga acgaattgtc atcattaaca
     8341 tcataataag aagaatcaaa gaaataatca tatcctaaat tcttgtatgt ctcgtttcga
     8401 ttccagaatg taccagcgtt cccatggaat accgcactag tgtaaccttg tgtttgactt
     8461 aaaatatcag gtgcagcttg gaaagtattt tttccgccta attgtgtgaa caaggagcct
     8521 tgattcaagc caaacaatga attttccatc agcgtttccg catcgcttgt ttttcctgct
     8581 tttacttgat ggaaaaagtt gtcaaaactg aacgtgctgt tgctgtggta aagactattg
     8641 atgaaaggtg taacttcatg ctggagacca ttctcatctg tcaatttata atcgatcagg
     8701 aattgctgga agctttctaa atggatataa atcacatttt ttcctttagc gagcccgaac
     8761 aaatcgtcat ttggtgctgc ataatgttct tttacataat tttctacttc tttcatatca
     8821 ttgggacttg cttctgcccg cacttggttt gtctggtaag tttgaacacc gtcataaacc
     8881 atgaaagcat tcaagccaag atatttgacc aaataatcac gagagaatgt tcgagtcagc
     8941 aattctggac gatcagcttc cgccataaaa agatttcctg agaaaatcat aactgctaat
     9001 gtagagatcg caaaagccat tcttgctcgg atcgggcgag ggtccaactt gatttttttt
     9061 gcaatcagca gaacagtcaa gataataaaa tcgatccaat agatcacatc atatgggcga
     9121 aacagtcgaa tggcactttc tcccagacca ctcgctactt tcccagcacc taaaatcgtg
     9181 ttgatcgtaa tgaagtccgt gaattctcga taataagtga cattcgaaaa aacaagcaat
     9241 gtcaaaagaa aataaatgac catcatcgtg atataagcaa ctctagggcg tctgatataa
     9301 agagcaattc caagtaataa caatgtagtt gcaatagggt tgataaacaa gataaagtat
     9361 tgaatgccac tttcgattcc gagatggaaa tcaactaaat aggcaaaaat cgttttcaac
     9421 caaagtaaaa cgactagtaa agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt
     9481 tttatacatt tcaagataat cttcctttct gcacttctta atcacagaag tgtcatgaaa
     9541 ccatgttaca cagctcactt attctcattt attttactag tatgacaata tagtgtcaat
     9601 tgaaaccaga agtttatgct gtttcattaa ggttttcttt aagcttcatg aaaaaatgag
     9661 cagtaagaca agtcctatgt tttttgttat acttgggaag agaaagctaa agcaggtgaa
     9721 ggaatgaaag taaaagtaaa aaaaagagca agcaatcgat tgaaaaaagg atatccactg
     9781 atccaaaaag aagaccttgc tcatccccaa aaagaaacca cgcaatgggt ggaattagtt
     9841 gattcgcaaa accaatttat cgcaaaagga tatctaggaa agcagaataa gggaatcggt
     9901 tgggtattga gccagcagaa catcccattt gatcaatctt tttttgaaga attttttgtg
     9961 aagtcaaaag aaaaaagaac tttctttttt tcagatgatt ctactaccgc ttttcgtata
    10021 ttcaatggag aaggagacgg aatcggcggt ctgattgttg attattatgc aggttatgct
    10081 gttttttctt ggtataacga aacgttatat gcacatcgtg aattattgat tcaagctttt
    10141 cgtgaaatct acccggaaat cgtaggaggc tatgagaaaa ttcgatttga atcagtaaat
    10201 ctgcctgaga gccagttcat ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa
    10261 aacggagtaa aatttgccgt ttatttaaat gaaggattaa tgactgggat ctttttagac
    10321 caaaaagaag tacggggatc attagcagaa ggtttcgctt ctgggaaaac agttttgaac
    10381 atgtttagtt ataccggcgc cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca
    10441 acaagtgtcg atctagcaaa acgcagttta aaaaaaacac aggaacagtt tgaagtgaat
    10501 ggtttggatg tcgatcagca aaaaatcatt gttatggacg tattcgagta tttcaaatac
    10561 gccaaaagaa aacaattttt atatgacatg attgtattgg atccaccaag ttttgccaga
    10621 aataagaaaa aagtctttcg cgtagcaaaa gattatgggg aactggtcaa ggatagtctg
    10681 gatattttag cagaagacgg gatacttatt gcgtctacaa atgctgcgaa tgtctcgata
    10741 gaacaattcc aaacaatgat tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa
    10801 cttgctttat atcgtctgcc aaaagatttc cagacgacgg ataccttttt agaaggcaat
    10861 tatctgaaag tgtttgttta tcaaattaaa aataaccatt agaaggagcg atgcagaatg
    10921 gtttgggtaa agggattgaa tataggaagc ggtagaccaa agatttgtgc gcctcttatc
    10981 ggggccgtcc gagaggaagt attacgtgaa gcagttcttg cacgtgaagc aggggccgat
    11041 ttagtcgaat ggcgagtcga tcattatcgt gaagtttttt cttttgatga agtagcagaa
    11101 acattggttc ttatccatga gatgcttgat ggactgccgt tattatttac gtttcgtacc
    11161 ttagctgaag gcggagaaca agacatatcg atccgtcagt atcgcgacct ttatgaacat
    11221 gtgatacaca caggattagt agatatggtc gatatcgagt tgttcaaagt agaaagtcta
    11281 ggaaaaagac tgatagagga aatcaagtct ttaaacattc ctttaattat cagtagtcat
    11341 gattttaaag aaacaccagc agaccctgtc ttgctttacc gattgaatgt tatggaacat
    11401 tttggagccg atatcggaaa actggcagta actcccaata atgaacgaga tgttttgcgg
    11461 ttgatggagt tgactagacg ggcaaatgct tttgtctcca tgcctattat tacgatgtcc
    11521 atgggaaatc ttggtaaaat ttctcgtctg gctggcggaa cgacgggttc cgttatgaca
    11581 tttggttcct taagcagcga ttctacttct gctccaggac aattaccggt caaagcattg
    11641 aagcaaatta ttcaattatt aaatgaaccg ttgtagaaca agtttgaggt gatatttttt
    11701 ggcaaagaaa aaaatagtaa aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc
    11761 ctatacagag cacgaatatg agtgggacga aagccaccta agtgcgtcat cggtcgctga
    11821 acagcttcca gaaagccaat ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac
    11881 ggggcctctt gttgctgtga taccgggaga agccgaattg aatctaaaaa aattagcaaa
    11941 agtttcaggg aataaaaaag tagaaatgct ccatttaaaa gatttggaag caacgaccgg
    12001 atacatacgt ggtggctgct cgcctatagg tatgaaaaaa ctatttccta cttatttaga
    12061 tcaaattgca gaaagctatg aacaaatcat tgtctcggct ggcagaagag ggctacagat
    12121 ggagttagca cctcaagata tctgtgcatt aacgagcgga caatttgcag atataaaaca
    12181 ataaatcata cgtataaaag aaagaaggag gccgggatgt tgtttgtcca gtctccttct
    12241 ttctttttta tacattatgc tctttgcccg gcattcattc ctgctacatg ccctgtagcg
    12301 aaagccgctg tgatattaaa gccgccagta taaccattga catccagtaa ttctcccgca
    12361 aaatacagac catcaatgat tttactctcc aatgttttag gataaatttc ctttagagaa
    12421 acccctccgc cagttacaaa tgatttctca ataggaaaag ttttgttgat tgatagctta
    12481 aactctttgc atagttgaac aaaatcttga atttgttttt gagaggcttg ttgtcctgtc
    12541 agatgatcca tttcaagccg ctcaagaaag aaaactaaca atcgctcagg caatatacca
    12601 tgccaagcat tgaccaagtt ctttttggtt gctttacttt tttctgtcaa tatatgaata
    12661 agctcttgtt tggtttgtgt agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt
    12721 ttctctaatt ctttattgac aaaacttgaa caacgcagtg cagccggacc agaaataccg
    12781 aaatgggtga aaagtaaatc catcgtatgt tcagtcaata cacgaccttt tttatttaaa
    12841 acgcgtaaag tgatgtcttg taaagaaagc ccttgaagtg ttttctcctg aatatacggt
    12901 tcatccgata tcaaggggga ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc
    12961 ttcacgatct tgtacccatc tccagtagaa ccagtagatg gataagtccg tccaccagtt
    13021 gttaagataa tggcaggagc atggaactct tcgaattccg tgcgtatacc gtaaatttta
    13081 tgttctttgt ggatcaattt ctctactcga gtagagaata gaagcgtcac atctagttct
    13141 ttcagacgat tgagcaatgc ttcgataatc gtttttgatt tatttgttac aggaaacatc
    13201 cgtccatgat cctcttcttt taaatgtacg ccttgtgatt caaagaagtt catgatatcg
    13261 aaattattcc attgcgcaaa cgtactatat aaaaactttc catttccagg aatatgtgca
    13321 atcaaatcat caacaggacg attgttcgta acattgcacc gaccaccacc tgtcatcagt
    13381 agtttttttc ctgctttttt gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct
    13441 gcactgatcg ctgccatcat cccgcttgtt ccagcgccga ctacgatcac atcatacagt
    13501 ttttccattc cctcacctca aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa
    13561 aatgttttga gccaaaagag aaaatgagct aaaatgaggg aagataagca aaggagcgtg
    13621 gaacgatgac aaacgcaaca gaatatgttc aagcaattca agaaaagcta catcaaaagg
    13681 atcaagggca agaagaattt ttacaagcaa tcgatgaatt catgcccacg gttatgtctt
    13741 ttttggacac tcatcccgaa tatatcgaaa agaatatctt agaactttta acggaaccag
    13801 aaagagttat tcaatttcga gtgccttggc aagatgatga aggaaactgg cgagtaaata
    13861 gagggtaccg tatccaatac aactcagcta tcggccctta taaaggtgga ttgcgcttcc
    13921 atcctagtgt caatctaagt atcttgaaat ttttggcttt tgaacagatt ttcaagaata
    13981 gtttaaccgg tctgccaatc ggtggaggaa aaggcggaag tgacttcgat cctaaaggaa
    14041 aatctgataa tgagatcatg cgtttttgtc aaagtttcat gctggaattg tcaaaacata
    14101 tcggcccatc gattgatgtt cctgcgggag atatcggtgt aggagctaga gaaatcggct
    14161 atttgttcgg cgaatataaa cgattgaaaa aatatgatac tggtgtgttg acaggtaaac
    14221 ctttggattt ttggggaagt aagatacgta cggaagctac tggttatgga ttagtgtatt
    14281 atgtcaaaca tctattgaac gaagaaaaag attcatttaa tcaaaaaaca gtttttgttt
    14341 ccggaagcgg gaatgtagcc atttatgcaa tcgaaaaagt gcaagagcta ggcgggaaag
    14401 ttgttacatg ttcagattct agcgggtaca tctatgatcc agaaggaata gatgtgaaac
    14461 tattgaaaga agtcaaagaa atcaatagac aacgtttgac ggaatacgca aaccaacgtc
    14521 cttctgctat ttatcatgct ggagaatctg tttggacatt gaaagaaaaa gcggatatcg
    14581 ctttgccatg tgctactcaa aatgaaatag acgaaagctt ggccgaaatc ttagtagaaa
    14641 atgggatcaa aatcgtagca gaaggcgcta atatgccatg tacagtaaaa gctgtttctg
    14701 ttcttcatga agcaggcgtg tggtattgtc caggaaaagc tgctaatgct ggaggagtag
    14761 cagtatctgc tttagaaatg agtcagaacg tccagcgatt gacttgggaa agagagcaag
    14821 tggatgagca gctggatcaa atcatggaaa cgatttacca tacgtgtcgc caaacagcag
    14881 ccgagtacgg cgacgaaaaa aatctgcttc ttggagcaaa tgttgctgga tttgaaaaag
    14941 ttgccaaagc aatggcagca gaaggtttag tttgaaaaat gaaactttct tggaaatatt
    15001 gtcagcattt tgtttatttg cttgaaaaat aataaaaaaa aacgtaaagt attacctgta
    15061 gagtaaacct ataaggagga gaacaaatgg cacacattca ttttgactat tcaaaagtag
    15121 caccgtttgt caacgaacaa gagttagggt acatgcaaag ccaagtaaca gcagcccaca
    15181 acgaattacg ggaaggtact ggcgcaggaa gcgacttccg cggctggatc gatttaccaa
    15241 cgaactatga caaagaagaa tttgcacgta tcaaagaagc agctaaaaaa atccaatctg
    15301 attctgaagt attagttgtt atcggtatcg gtggttctta tttaggcgct cgtgcagcga
    15361 tcgacttctt gaatcacaca ttcttcaatt tattagatag caacacaaga aaagcaccac
    15421 aaatcttctt tgccggaaat tcaatcagct caacatatat tgctgacttg atcgaagtaa
    15481 tcggtgatcg cgacttttca gtaaacgtta tttcaaaatc tggaacaact actgaaccag
    15541 cgattgcgtt ccgtgtattc aaagagcttc ttgttaagaa atatggacaa gaagaagcaa
    15601 acaaacgtat ctacgctaca acagataaag caaaaggtgc agttaaagta gaagcagatg
    15661 cagaaggttg ggaaactttc gtgattcctg atgacatcgg cggacgtttc tctgtattaa
    15721 ctgctgttgg tttgttacca atcgctgtta gtggtgcaga tatcgatgca ttgatgcaag
    15781 gtgccgctga tgcaagcaaa gcatactcaa gcgacaaact agaagaaaac gaagcttacc
    15841 aatacgcagc aatgcgcaac attctttacc gtaaaggcaa agtaactgag ttgttgatca
    15901 actatgaacc aggaatgcaa tacttctcag aatggtggaa acaattgtat ggtgaatctg
    15961 aaggaaaaga ccaaaaaggc atctatcctt caagcgcaaa cttctcaact gatttgcact
    16021 ctttaggaca attcatccaa gaaggacgtc gcaatatttt tgaaacagtt gtgaaggttg
    16081 aaaaacctcg taaatcaatc acaattcctg aacaagcaga agatttagac ggtctaggct
    16141 acttgcaagg aaaagaagtg gacttcgtaa atacaaaagc atttgaagga acattgcttg
    16201 ctcatacaga cggtgacgtg cctaacttgc tagtcaaaat tccagaaatg gatgcttata
    16261 cattgggtta tacaatgtac ttctttgaaa ttgctgtagg tatttcaggt tacttgaatg
    16321 gaatcaaccc attcgaccaa ccaggtgtag aagcttacaa gaaaaacatg ttcgcacttc
    16381 taggcaaacc aggctttgaa gatcttgcta aagaattgaa cgaacgcctg taagataagg
    16441 gtttttcgct agatttaaat aatcagtgat agaaacatgt tcgctttaaa atgaaaactg
    16501 gtacccattt aggtactcaa acatactgca tcgtttattc agaataaggg gctgtgacaa
    16561 gacgtttgtc acagtccctt gtttgtatag aaatcgattg attgccttgc tctttagttt
    16621 gttttaggac tttaagaaca gtgaggctga cttcgaaggt taattctgtt tttttgtttt
    16681 ctagaatcag taagcaaaaa agcactcact tcgagtgctt ttttgcgata tattcttagc
    16741 ttaagatagg cctaattttt cttttacaga tgagggtacc ttatcgctgg taattacttt
    16801 tttctctatg atccgctttt cgcttgcttt caactgcaag atcgttcctt cggggtaagc
    16861 agaaccattg gcagagtcac cagaagtaat gacatcgaaa tctaactgtc ttttttcacc
    16921 gttttcagca taagcatcta cgttgtattt aaaacctctt ccaattactt ccccattatc
    16981 tgctttgata tcagtttctg caggcattgg agctttgact accgcataat aatctttacc
    17041 tacataagta gactgatagt agtcccaacc tttccatcct ataaaagcaa tcaatatgat
    17101 aacgatcaac cctaaaattt ttttcatgaa agatctcctt ttttattttt attatacccg
    17161 tggtgctagt taattttaat atcctaaaga atatctcaaa gttccttcaa aacgtcgttg
    17221 tgctcgactt aatagaacac tgtaagccag taatatgctt tcaaatctac tctctagccc
    17281 tttaacagaa cgtgagttga aattttgaca acctaatttt tctaaggaag aaaaaacagt
    17341 ctcaactg
//
LOCUS       DAZULO010000057        16697 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000057 DAZULO010000000
VERSION     DAZULO010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.058"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..787
                     /locus_tag="ACG8OA_001836"
     CDS             278..787
                     /locus_tag="ACG8OA_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4976607.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ACG8OA_001837"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ACG8OA_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4976608.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="ACG8OA_001838"
     CDS             2322..3302
                     /locus_tag="ACG8OA_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4976609.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="ACG8OA_001839"
     CDS             3424..4842
                     /locus_tag="ACG8OA_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFS4976610.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="ACG8OA_001840"
     CDS             4913..6382
                     /locus_tag="ACG8OA_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4976611.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="ACG8OA_001841"
     CDS             6392..6859
                     /locus_tag="ACG8OA_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS4976612.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /gene="scrK"
                     /locus_tag="ACG8OA_001842"
     CDS             6895..7767
                     /gene="scrK"
                     /locus_tag="ACG8OA_001842"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS4976613.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="ACG8OA_001843"
     CDS             7848..10382
                     /locus_tag="ACG8OA_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4976614.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="ACG8OA_001844"
     CDS             10538..11311
                     /locus_tag="ACG8OA_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4976615.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="ACG8OA_001845"
     CDS             11304..12095
                     /locus_tag="ACG8OA_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4976616.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="ACG8OA_001846"
     CDS             12105..12596
                     /locus_tag="ACG8OA_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS4976617.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="ACG8OA_001847"
     CDS             12614..13024
                     /locus_tag="ACG8OA_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4976618.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="ACG8OA_001848"
     CDS             13028..14428
                     /locus_tag="ACG8OA_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFS4976619.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="ACG8OA_001849"
     CDS             14451..14825
                     /locus_tag="ACG8OA_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4976620.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(15381..16460)
                     /locus_tag="ACG8OA_001850"
     CDS             complement(15381..16460)
                     /locus_tag="ACG8OA_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4976621.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT         5580 a         2529 c         3423 g         5165 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt cgtaaatttc aagtttaagt tagccaccca tggtgacccg gttgctcgtt
    15241 accaccaacc gtctaatttt tctaatcgag tgggcccttg acaatgagct gaggcatggt
    15301 agtctcccga ctggctggcc ccggccagct ttccagacac aagccatgcc gagggaggct
    15361 cgttgtcaag ggcccacgtg tcaagccgtc aaccgttcct gcctaaatgc ctggacgaac
    15421 gtgtcatgcg gcgttgcata gcccaaaatc tttcgcgggt aatcgttcat ccaacgctga
    15481 atccgcaaga tttggttttc gctcacgttg ccgattgggc tgcccttggg gatgaaacgg
    15541 cgaatcagct tgtgttggtt ttcgctcgtc ccgcgctccc aggaagcata ggggtggctg
    15601 aagtagatgt ttgccacatc ctgcaaggca tcgtgcagtc ccgcgaactc cgagccgttg
    15661 tccgaggtaa ccgtcttgaa cagctggccg aagtacgcac cagcccgttc tctcagctgt
    15721 cggaccgctt ggttgaccga ttcggcatcc ttcccggcca gcttcagaat gacctcgaag
    15781 cgtgtctgcc gttccaccaa cgtcagcagc acggcatccg acttggtctt gtttcctacg
    15841 accgtgtcga cttcccaatg gccgaaggtt ttccgggtac caaccgcctc cgggcgctct
    15901 tcaatggatt tccctagcac ccgtttattc gggcggttgc catgacggga cgatttcggt
    15961 ttgcgggaca gtttttctag caggtccata ttcttcgttt tcatgatgcc acggtcgatc
    16021 cactgataga gggtggtggt cccagggata atggccgggt cgaacaattc gtgttttctg
    16081 gcaaagccca ccaccacgtc cggggaccat ttttccaaca gcatcatgtc gtccgcccat
    16141 tcaatgaagg cgtccgtgtc ggcccataag ggccgtctgc cgcagttcaa gcggttcttc
    16201 tcgtaggccg cctgcccggc atcggcgtcg tagacgtcat gataatagtc gtagaccttc
    16261 ccgttctgat tctggcggtt aagctgagta acggtcccgc gtttgatttc gttgtttatg
    16321 gtctgcggca cgcagcccag gacatccgca acctgacggt tggaatagtt ttcctttttc
    16381 aggatggcga tttggcaacg ttctgagtag gttaggtgtt ttcccttgcg tgaaactgtg
    16441 ttatcatggt tttgcgtcat gtgaattcat ccttgtctat tgagagttgt ggtaacttca
    16501 atataacatg gaattcacgt ggcgtttttt tatttccaaa tggctaagtt aattataaaa
    16561 tccgcccttt ttttattttg aaaaattttt agtatttttc gaaaaatgct cactcctaga
    16621 gtaagcgccc catagaacag taccttacta acaaatgcac ctcaagatat acttgagcta
    16681 tcaagaagtc tggaggt
//
LOCUS       DAZULO010000058        16636 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000058 DAZULO010000000
VERSION     DAZULO010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.059"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..727)
                     /locus_tag="ACG8OA_001851"
     CDS             complement(29..727)
                     /locus_tag="ACG8OA_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFS4976622.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(720..2072)
                     /locus_tag="ACG8OA_001852"
     CDS             complement(720..2072)
                     /locus_tag="ACG8OA_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFS4976623.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2295..3695)
                     /locus_tag="ACG8OA_001853"
     CDS             complement(2295..3695)
                     /locus_tag="ACG8OA_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS4976624.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3856..4593
                     /locus_tag="ACG8OA_001854"
     CDS             3856..4593
                     /locus_tag="ACG8OA_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFS4976625.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4718..5197
                     /locus_tag="ACG8OA_001855"
     CDS             4718..5197
                     /locus_tag="ACG8OA_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFS4976626.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5194..5961
                     /locus_tag="ACG8OA_001856"
     CDS             5194..5961
                     /locus_tag="ACG8OA_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFS4976627.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6191..6760
                     /gene="lepB"
                     /locus_tag="ACG8OA_001857"
     CDS             6191..6760
                     /gene="lepB"
                     /locus_tag="ACG8OA_001857"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS4976628.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6864..7466)
                     /locus_tag="ACG8OA_001858"
     CDS             complement(6864..7466)
                     /locus_tag="ACG8OA_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS4976629.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7572..7802
                     /locus_tag="ACG8OA_001859"
     CDS             7572..7802
                     /locus_tag="ACG8OA_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976630.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7793..8041
                     /locus_tag="ACG8OA_001860"
     CDS             7793..8041
                     /locus_tag="ACG8OA_001860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4976631.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8147..8731
                     /locus_tag="ACG8OA_001861"
     CDS             8147..8731
                     /locus_tag="ACG8OA_001861"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFS4976632.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8734..9807
                     /gene="prfA"
                     /locus_tag="ACG8OA_001862"
     CDS             8734..9807
                     /gene="prfA"
                     /locus_tag="ACG8OA_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFS4976633.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9800..10639
                     /gene="prmC"
                     /locus_tag="ACG8OA_001863"
     CDS             9800..10639
                     /gene="prmC"
                     /locus_tag="ACG8OA_001863"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFS4976634.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10632..10811
                     /locus_tag="ACG8OA_001864"
     CDS             10632..10811
                     /locus_tag="ACG8OA_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976635.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10833..11858
                     /locus_tag="ACG8OA_001865"
     CDS             10833..11858
                     /locus_tag="ACG8OA_001865"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFS4976636.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12033..13277
                     /gene="glyA"
                     /locus_tag="ACG8OA_001866"
     CDS             12033..13277
                     /gene="glyA"
                     /locus_tag="ACG8OA_001866"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFS4976637.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13430..14377
                     /locus_tag="ACG8OA_001867"
     CDS             13430..14377
                     /locus_tag="ACG8OA_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFS4976638.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14511..15140
                     /gene="upp"
                     /locus_tag="ACG8OA_001868"
     CDS             14511..15140
                     /gene="upp"
                     /locus_tag="ACG8OA_001868"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFS4976639.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15279..15521)
                     /locus_tag="ACG8OA_001869"
     CDS             complement(15279..15521)
                     /locus_tag="ACG8OA_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976640.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15642..15714
                     /locus_tag="ACG8OA_001870"
     tRNA            15642..15714
                     /locus_tag="ACG8OA_001870"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15675..15677,aa:Thr,seq:cgt)
     gene            complement(15833..16471)
                     /locus_tag="ACG8OA_001871"
     CDS             complement(15833..16471)
                     /locus_tag="ACG8OA_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFS4976641.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         5672 a         2959 c         3335 g         4670 t
ORIGIN      
        1 ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga tagggcagat
       61 ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct gccaggttct
      121 catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg taaatgacac
      181 cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc aaaggtcgtt
      241 caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa gtttcattga
      301 attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga cttaacgtgt
      361 agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg tagtagtgac
      421 ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg atatagttga
      481 tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca aagtcttgac
      541 caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt atactaacga
      601 tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc aaaaggtttc
      661 cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg agttcataat
      721 tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt acgaagctgc
      781 aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc tttgattttt
      841 tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat cccggcaact
      901 tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc tgggatatcc
      961 atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat tccgtctgcg
     1021 taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc aatcatatcg
     1081 attacttgat tgatacctac aggatttttg acaagtacta atgtacattt tttcccattg
     1141 atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc ttgtttgatt
     1201 ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc attgtagaca
     1261 ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa atctgctgac
     1321 acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg tttgaagtca
     1381 caatttggac agtaatattt ccctaagttt gcataagtga tcattttata atgcaagatg
     1441 tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg ttcacgatct
     1501 tcttcatggt caaagccata atattttcta ggattgactg tttcgataga attgaagatt
     1561 ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc gtctacaatc
     1621 attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa aatatttgta
     1681 aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc ttcatctatt
     1741 tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa agtcgataca
     1801 attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa ttgctggcgc
     1861 aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt gacaacgacg
     1921 acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag cgctagttta
     1981 cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga cgtttttcca
     2041 actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac gatttgctga
     2101 cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc ttttcttatt
     2161 cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat gatttaacaa
     2221 aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta ttccctctta
     2281 cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata gcctagatct
     2341 cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg cccaataaac
     2401 atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat ccctaaagat
     2461 aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag cgtttgttct
     2521 tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag cataaagaca
     2581 acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt gatgttatct
     2641 tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt tgttcgaatc
     2701 ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa aatgatcagt
     2761 gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg agtcgtaatc
     2821 gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa taaatggtcc
     2881 ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa agctaacaca
     2941 gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg tttgtataat
     3001 agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa aatcaaaaac
     3061 atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc cccagtgacg
     3121 tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa aacggcatca
     3181 cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt cccaaaaagt
     3241 aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat accagacaaa
     3301 cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat ccgtcgttta
     3361 gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac ttgaaataat
     3421 atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa agtcgatagt
     3481 ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga aacatggcta
     3541 tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt catcaatata
     3601 taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt ctgtcctcgc
     3661 cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg acaatagtcg
     3721 tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa cagataacaa
     3781 tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga aaaaagaagg
     3841 aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa aaatcaaaaa
     3901 ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga agaagtaaag
     3961 cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt ggaattcatg
     4021 tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc taaaagtgag
     4081 tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga gaaaactgac
     4141 catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa aatggtttta
     4201 acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg cttcaatatg
     4261 cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt tggaagtgcc
     4321 ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat ggatatccaa
     4381 aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg ctatgaacgc
     4441 tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg ttgcgtgttc
     4501 gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa agcttactta
     4561 aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa acgtaagaaa
     4621 aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt agtaaagtaa
     4681 ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag cacctatttt
     4741 gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag gtattgtgaa
     4801 tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa caacaccaac
     4861 tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa ccactacgac
     4921 aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata caactgacag
     4981 ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa ccaatccaag
     5041 tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga ctgtagatga
     5101 taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt caggaaatac
     5161 ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga ccttatcaac
     5221 ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa ccgtggatat
     5281 acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg actttggcaa
     5341 aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct tgtttatttt
     5401 actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga tgtcactcag
     5461 ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga atcgatagat
     5521 aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta tgacgaagat
     5581 tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga gatcaaaaca
     5641 aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta tccttattac
     5701 tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag ctactcggac
     5761 atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga acctgtcgga
     5821 ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga gccttatcgt
     5881 ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac cagtggttta tagtcgtagc
     5941 caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg cgataaaaac
     6001 gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt ttatataacg
     6061 agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca ttgtagaata
     6121 aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa atacaagtag
     6181 gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg tattagcggc
     6241 tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc attcgatgga
     6301 ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact atgaacgaat
     6361 ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta aacggatcat
     6421 cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta atggcaaaaa
     6481 gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata agttacaagg
     6541 agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca cctttacgag
     6601 tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata accgcttgat
     6661 ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag gaaaagttgt
     6721 tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata aaaagaagct
     6781 gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt cttggagttg
     6841 ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa aagcgaagcg
     6901 tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc agagacaaag
     6961 cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg gactttcgga
     7021 tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc aggagctaaa
     7081 atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag tacaagtgat
     7141 agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc gtggatcgtt
     7201 cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag catagtttct
     7261 gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact aacaagccca
     7321 gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag ataagtagtt
     7381 agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata atggttagcg
     7441 atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt tgaaatttta
     7501 acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa aagggagaat
     7561 ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga aagtgtgtcc
     7621 gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg gaaaaatata
     7681 tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa tatttaggct
     7741 ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt gtatgggaat
     7801 aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag cagaaggtta
     7861 cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc actactctat
     7921 atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg aagtttttcc
     7981 aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg aacaaaagtg
     8041 agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa aaatgctgaa
     8101 aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg cacaactatt
     8161 ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag tcgcacataa
     8221 ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata caagagatgg
     8281 tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga tatttgaaga
     8341 aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg tgctgatcga
     8401 tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg tggacgaatt
     8461 ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt tatttgaagg
     8521 atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa tctgttggtt
     8581 ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg tttatgaagg
     8641 cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa aacattactt
     8701 tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca agcaattgaa
     8761 gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc tgatacaaag
     8821 cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga agtttatcgt
     8881 cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg tgaaaaactg
     8941 gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa agaaaaagaa
     9001 gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga cgataaaaat
     9061 atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt cgctggcgat
     9121 cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga agtgatggaa
     9181 gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac cggagacaac
     9241 gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt tccttcaact
     9301 gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc agaagctgaa
     9361 gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca tgcttcaggt
     9421 gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca cttaccaaca
     9481 gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga aaaagcaatg
     9541 aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag cgagtatgat
     9601 gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg gacgtataat
     9661 ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa attagatcag
     9721 attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga tcaaacatca
     9781 aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt cacgggcttc
     9841 ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat ttttggaacg
     9901 aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt cagaagaaga
     9961 acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac ctcagtattt
    10021 gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa cactgatccc
    10081 tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg gtgtaccgtt
    10141 agaagtgata gatattggga cagggactgg agcaattgct attagtttga aagcagcaag
    10201 aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag tagcgaaaga
    10261 aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat taggaccagt
    10321 aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc ggaatgaatg
    10381 ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt ttgctgagaa
    10441 cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac taaaatcaaa
    10501 tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa aaatatttca
    10561 gcaagcattt ccagataaaa aagtggcgat aaaaaaggat ctttttggca atgaacgaat
    10621 gatccaagtt tatggttgaa gttgtggatg aaaaattttt tatttgtttt tttattataa
    10681 aaaaacactt atttgacaag gatgtaaacg actttttggt ttatcaacaa gtttatccac
    10741 tattgacaaa tagattatcc acaaacagtg gaaaacttga ggataaactc agacaaaata
    10801 agaatgtatg aaaaaggggt tgtggataaa ctgtggaaac gaaacgatat gatgaaacag
    10861 aattgcaaga ggcagccgaa gcactgaaag aaggagaact tgttgctttt cctaccgaaa
    10921 cagtatacgg attgggcgcc aacgctcttt taccaaacgc tgtcaagaaa gtgttttctg
    10981 taaaagggag accgcaagat aatccattaa tcgtccatgt cgcctctttt gaacaagtaa
    11041 aagagtatgt ggacaacttt catccggcag cagagaagat tgtgaagaac ttctggcctg
    11101 gaccattaac tttgatattt aagatcaaaa aagacacttt gccctcagtt gtgacgggag
    11161 gattatccac agctgcattt cgtataccgg ataacaaaaa aacactagaa gtaatcgaac
    11221 tttccggtgt tccgcttgtc ggtccaagtg caaatacgtc gggaaaacca agtccaacga
    11281 ctgcagatca tgtatatcat gatctacaag gaaaaattac cgggattatt gatgatggag
    11341 ctacgcgaat tggtgtagaa tccactgttc ttgatttaag tgatccaaca gccatgccta
    11401 tgattttacg accaggtgca gtaacgaaag aacaaatcga agcagtcatc gaatctcctg
    11461 ttgctattga tcagcatctt gtaaaagaaa atgaaacacc aaaagcccct gggatgaaat
    11521 acaaacacta ctcacccgat acacgtgttc tgatggtcag agatggcgat tggtcaactg
    11581 cagttcaatg ggcaaaaaat aagaagatcc gtgcaggtgt catagcaagc cccgaaattg
    11641 cggatcaagt gcggacagat actgcagctg tttatatgta taacgacaac tcagtagaag
    11701 cggcggctaa aggattgttt gcaggtctaa gaggattaga tgaaccaaca cttggattag
    11761 atttgatttt tgtacaagtg tatccagaaa ctggattggg gaatgcttac atgaatcgct
    11821 tgaaaaaagc agcaggacaa aattattttg aaaaatgatc aattggttat tgtcgcattc
    11881 ttttagtatg atacaatgct atcaagtaaa tcttctatca gtgaacgaaa aactaagcgt
    11941 aactaatcgt aatagaagag atacgaatat ttggcaattt gtttttgatg ggaaaaataa
    12001 aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg tttgaccctg
    12061 atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg gagctgattg
    12121 cctctgaaaa ctttgtctcc gaagcagtga tggctgcaca aggaagtatc ttgacaaata
    12181 aatacgcaga aggttatcca gggcatcgct attatggtgg gtgtgaattt gtcgatatcg
    12241 ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt gcgaacgtcc
    12301 agcctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta gaaccagggg
    12361 acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga tcaccagtca
    12421 acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca acagaagtta
    12481 ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg atcgtagcag
    12541 gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc gcagatgaag
    12601 taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca gcaggacttc
    12661 atcctaatcc agttccttat gcagatatca caacgactac cactcataaa acattgcgtg
    12721 gccctcgagg cggcatgatt ttgacaaacg atgaagcatt agcaaaaaaa ataaatagtg
    12781 cggtatttcc tggtattcaa ggcggccctc tagagcatgt gattgcgggt aaagcagtag
    12841 cattcaaaga agcattagat cctgcattta aagaatatag tgaacaaatc atcgccaatg
    12901 caaaagcaat ggtcaaagtt ttcaatcaag caattggaac cagagtcatt tcaggggcga
    12961 cagataatca tctgatgttg atcgatgttc gcgagttagg tataaatgga aaagaagcag
    13021 agagcatttt ggacagtgtg aatatcactg taaataaaaa ctcgattcct tttgaaacac
    13081 taagtccatt caaaacaagc ggtattcgaa tcggcacgcc agcaatcact actcgcgggt
    13141 tcaaagaaga agatgctgta aaagtggctg aactagttgt caaagcatta caagcaaaag
    13201 atgataacgc tcaactagac gaagtcaaaa caggtgtacg cgaattaact gaaaaatttc
    13261 cgttgcataa aaaataaaaa tttaaattcc attagtaaac agaaaagttc aatgttgggc
    13321 ttttctgttt attttttggg aaaacggatt ctttgacaat atcggtgatg ctctctagta
    13381 tatgaggtgt aattttagtc ttcacactaa atctcaggga ggaaaagtca tgactgatcg
    13441 tatctcgtta gaaaaggaag cagtagcatt cagtgatgca aatgcgcctc atccgcgtat
    13501 ctatgaacta gcaccggaag aaggaagagt tttactagaa gaagtacaag aatcacctgt
    13561 gaacaaatat tccgtcgata ttgaagactt gatggtggat actggcgaat ggggaaaact
    13621 caatattcgt ctcgtacgac cagaagggaa tatggataaa ttaccagtta ttttttatat
    13681 tcatggagca ggctgggttt ttggcagtgc tcaaacccat gataaattga tcagagaact
    13741 tgcggttcgt acgaattccg tcgttgtctt tccagaatat agtcgatcac cagaagcaaa
    13801 atatccaaca gcaattgaac aaaattatgc tgtgttgcaa cagctgaata aattagccaa
    13861 gtcaaaaaat ctggatctcg agaatctaat cgttgcagga gattcagtag gtggtaacat
    13921 ggctacagtc atgacgatct taacaaaaga acgaaagggc ctcccaatat ccaagcaatt
    13981 gctgtattat ccagtcacaa acgctgattt tgatacagat tcctatcatg aattcggaga
    14041 aaactatttt ttgacaagag aaggaatgat gtggttttgg gaccaatata cgacgaatga
    14101 agaagagcgt gctgaaatca cagcttcgcc tttgagagca aatataaatg aactaaatga
    14161 tcttccttca gcaatgattt taacgggaga agcggatgta ttaagagatg aaggtgaggc
    14221 ttatgcgcgc aaacttcgtg atgcaggggt tcctgttaca caagtacgct tccaaggaat
    14281 catccatgat tttgtcatgg taaatgctat ggatcagaca aaggcaacgc gtgcagcaat
    14341 ggcattatct acttcttgga tcaagaaaaa agaataagct ttgcttgtcc gcaggaaaac
    14401 ttcttttcaa aaacggattt tcttaggaaa aatggcgagt ttgactagtc aatcacttga
    14461 ttttgtttta caattaaaaa gaaacgacag aacaaaggag agcttaaaaa atgggcaaat
    14521 ttcaagtgat cgatcatcca ttgattcaac acaaactaac tattattcgg gacaaaaact
    14581 gcggaacaaa ggtttttcgt gaagtagtcg atgaaatcgc tatgttgatg gcatatgaag
    14641 tatcacggga tatgccattg gaagacatcg tgatcgaaac accgatcact gaaacaacac
    14701 aaaaaacgtt gtctggtaaa aaagtagcga ttattccaat cttgcgcgca ggtattggaa
    14761 tggttgacgg tatcttggaa ttgattccag ctgctaaagt gggacatatc ggtctttatc
    14821 gagatgaaga aacattggaa ccacatgaat atttcgtcaa acttccagaa gacattgatg
    14881 cacgtcagtt gtttgtagta gatccaatgt tggcaacagg cggatcagca atcatggcaa
    14941 tcgatgcatt gaaagaacgt ggcgcgtcga acattaaatt tgtttgtttg gtagcagcac
    15001 ctgagggagt aaaagctttg caagaagcgc atcctgatat tgacatctat acagcttctc
    15061 tagatgaaaa attgaatgaa catggttata tcgttcctgg actaggggat gcgggtgacc
    15121 gtttgtttgg tacaaaataa gtaaatacga aaaagggtgt gacacaactt ttgtcacatc
    15181 cttttttggt catttttaac gtttgatgca gaacagcttt ttcacagctt tcgtttgcat
    15241 ataaaaaaac catcccaaaa atgcagagat ggtcaaattc aaatcttttt tagttctttg
    15301 tagttaacga ttactacacc cttactttgc gtcatcaagt tcgcattttc tgcagtttca
    15361 tcaatctcaa caacagcgga atttttcaat tgctttgaaa tcgttccagt ttgcgacttg
    15421 ccgtgaattg tgaacattac gtgagtattg atcttgaatt tttcaccaat ttccgatggt
    15481 acgatagttg tatcaaattt tccaaaagtt gccattttca taaaacctgc ctttcacatc
    15541 aattatacca cgttttatga aaaatatcat gaaattaaac taattttcca tcggactctt
    15601 tacaatcagc caaatagagt atataatttc ttttggataa ggccagctta gctcagttgg
    15661 tagagcaacg cactcgtaac gcgtaggtcg taggttcgat ccctatagct ggcattaaaa
    15721 gagaagacag atatatcaag gatttttaat ccattgatac atctgtcttt ttgtttaaag
    15781 gaaataaagt atgctttctt agcaatagag catgtattat ttctataaaa atttaggaga
    15841 ataaaacgac tagcaatata gctgtgaaat caaatatcac atgaactgcc caacttgtaa
    15901 tgatcgagtt gcttttaatg gcagcttggt tgaataacaa tcttgccacc ccttgtatca
    15961 aaaggatgtg tgctacagtg tgtaaaatat ttccattatc ataagtactg taatggataa
    16021 atccaaagat gattgctgac aagaaactag cgacaggaat gctgaactta ctagaaaaaa
    16081 tagcgagaaa atacactgag aacaattctt ctccaagtat catgataggc aaaataataa
    16141 ataataacgg gctgaaagcc tcatttattg gattgcctgt taaaccccat ttcaaaattt
    16201 gttgcaaaaa taccgagact gcggcactga ttactacatt gataaaaaaa tatttggcta
    16261 tatttttgac aggagcgaca ggtttcgaga acattttttt cgtatctttt ttaccaaaaa
    16321 caagtgtaaa tgctataaat ccagtgatag ttaaagaaaa aatgctaata atggatggcg
    16381 caaatagcaa ccctaacaaa ccgagtacaa cgacaatagt gcccaatatc ccttttttaa
    16441 gttctatttt ttttcctaaa atgttattca tgataataac tcctactatt cagtttttga
    16501 agatagagtc agtatacatg aagagttaaa ataggcaatg tttttttatg gatatttgta
    16561 ttgctaaaaa tttatcttat atattcattt tgattatact acttttattt gtaaaaattt
    16621 tctagcaaaa ccaagt
//
LOCUS       DAZULO010000059        16567 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000059 DAZULO010000000
VERSION     DAZULO010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.060"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..795
                     /locus_tag="ACG8OA_001872"
     CDS             <1..795
                     /locus_tag="ACG8OA_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4976642.1"
                     /translation="FYQYMLNDNCTANTVIHYHAFIRKALQEAVITELISTNVADKVR
                     KPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGLKWSAIDFLN
                     KKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAKWQQDNRILL
                     GDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRHSNASIMLDS
                     GQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            1369..1938
                     /locus_tag="ACG8OA_001873"
     CDS             1369..1938
                     /locus_tag="ACG8OA_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFS4976643.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1925..2785
                     /locus_tag="ACG8OA_001874"
     CDS             1925..2785
                     /locus_tag="ACG8OA_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4976644.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2975..3679
                     /gene="yycF"
                     /locus_tag="ACG8OA_001875"
     CDS             2975..3679
                     /gene="yycF"
                     /locus_tag="ACG8OA_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFS4976645.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3684..5519
                     /gene="walK"
                     /locus_tag="ACG8OA_001876"
     CDS             3684..5519
                     /gene="walK"
                     /locus_tag="ACG8OA_001876"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFS4976646.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5516..6832
                     /gene="yycH"
                     /locus_tag="ACG8OA_001877"
     CDS             5516..6832
                     /gene="yycH"
                     /locus_tag="ACG8OA_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFS4976647.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6833..7702
                     /locus_tag="ACG8OA_001878"
     CDS             6833..7702
                     /locus_tag="ACG8OA_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFS4976648.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7757..8566
                     /locus_tag="ACG8OA_001879"
     CDS             7757..8566
                     /locus_tag="ACG8OA_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4976649.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8669..9298)
                     /locus_tag="ACG8OA_001880"
     CDS             complement(8669..9298)
                     /locus_tag="ACG8OA_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4976650.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9468..10760
                     /locus_tag="ACG8OA_001881"
     CDS             9468..10760
                     /locus_tag="ACG8OA_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4976651.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10836..11498)
                     /locus_tag="ACG8OA_001882"
     CDS             complement(10836..11498)
                     /locus_tag="ACG8OA_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS4976652.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11723..12007
                     /gene="groES"
                     /locus_tag="ACG8OA_001883"
     CDS             11723..12007
                     /gene="groES"
                     /locus_tag="ACG8OA_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFS4976653.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            12058..13683
                     /gene="groL"
                     /locus_tag="ACG8OA_001884"
     CDS             12058..13683
                     /gene="groL"
                     /locus_tag="ACG8OA_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFS4976654.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(13772..14911)
                     /locus_tag="ACG8OA_001885"
     CDS             complement(13772..14911)
                     /locus_tag="ACG8OA_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4976655.1"
                     /translation="MWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWKKAQ
                     KILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHISDD
                     TIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPALAVK
                     IHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRYGEL
                     LALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIESVIA
                     DNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSHVSL
                     LSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(15022..15606)
                     /locus_tag="ACG8OA_001886"
     CDS             complement(15022..15606)
                     /locus_tag="ACG8OA_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFS4976656.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(15733..16164)
                     /locus_tag="ACG8OA_001887"
     CDS             complement(15733..16164)
                     /locus_tag="ACG8OA_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS4976657.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(16182..16502)
                     /locus_tag="ACG8OA_001888"
     CDS             complement(16182..16502)
                     /locus_tag="ACG8OA_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4976658.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
BASE COUNT         5423 a         2830 c         3369 g         4945 t
ORIGIN      
        1 ttttatcaat atatgttaaa tgataactgt acagcaaata cggttattca ttatcacgca
       61 tttatacgca aagctcttca agaggctgtt attacagaat tgataagtac aaatgtagca
      121 gataaagtaa gaaagccaaa aaagaaacaa tttgtttctc aagtttataa tcaacaagag
      181 atcttgaaat tacttgacat tttgtcagaa gaaaaactct atttggtagt tcttttaaca
      241 gcattttatg gattgcgaag aagtgaagtt ttaggattaa aatggtctgc tattgatttt
      301 ttaaacaaaa aaatcatgat aaaccatgta atcatagaaa atccagagaa tatgtcacag
      361 ttaataaaga aagatcaaac aaagaactct tccagctatc gtacattacc tttggttgaa
      421 acgattgaac gagcattagt aaatcaagct aaatggcaac aagataatcg tattctttta
      481 ggagatgatt atattttaga agataatgag tacgtattta ctatggaaga tggacggttg
      541 atgaagcctc aatatgtaac gcatcgtttg agtaagttga ttaagaaaaa tggattgaaa
      601 aaaattcgct ttcatgactt gcgtcattct aatgcttcaa taatgttaga tagtggccag
      661 aatatgaaat ccattcaaga atggctggga catgcatcgt attcgacaac tgctaattta
      721 tacacgcatt taaccgcagg agtaaaagat cgtgcagcag aagcattaga aaacgcattt
      781 gggtttaaag aataaaaaaa caaaaaacaa tatctttggg gtatcgcttc ttgtttttag
      841 aatttttttg ttggcaattt gttggcagta tgttggcaat gaaaaaattc acaaactaaa
      901 aacctattta taaagcttta taaaactttt ttcacaaata gagcatttgg ttaagagtca
      961 gctgctctac caactgagct aacggcccgt acaaaaagta cttaataata gtagcactaa
     1021 gaattttaaa atgcaagact acatttaaag aaaatcagaa aaatctttac ttccttatag
     1081 gaaatcattc atagattatt cacatttgct cgctattata taagcatacc aacaaacaaa
     1141 gttcttgcaa gagaacaaac cctaacactt ttttcatttt ttactccttt cccgccgtct
     1201 ccccggcggg tctcctgttc ttaacgataa aagacaagaa ggcttagcct tcttgttttt
     1261 tgttttcatg gaatcccgct aatcaaatca cctagttaaa gcaactagat tgaatgttta
     1321 tcataagtct gatatcatgt ttatgaaatg aaaaaaggag cggttatgat ggaaaaaatg
     1381 cgtcagcaat taatggagtg ggggaaggaa ctagaaacat tccgtctgcc tcactgggaa
     1441 gaactccctg acttagaact ttatatggac caagtcagaa cgttagttga tcgttatctt
     1501 tcacctgtga tacaaggaga aaagcatcca ttgcttacct cttctatggt aaacaattac
     1561 gttaaactag gtttgatacc agcaccagtt aaaaaaagat ataacaaaga acacgtagca
     1621 tttttacttg caatcactac gttgaaacaa gtattgacta ttcctgaaat caaagaaggg
     1681 atattgttcc aaggaaaaac agtaggaatt cgtgaagcct ataatctatt ttgcgatgaa
     1741 caagaagcag ctgtgtggat ggtcagccaa ttagcacaag gtaaatctca tccgcaaaaa
     1801 ttcgaccagg caacccctgt agaatatatt gctgtaagag cggcaacttt atcgtttgca
     1861 atgaaacttc ttgcggaaaa aacgattgtt ctagaaaccg aatatttaaa agaggagaaa
     1921 acaaatgaaa aacaataaaa tcgcaatttt agtagattca ggtacggatg ttccagaaga
     1981 attgataaaa aaatacgaga tgtatatgat cccattgaaa attatttata aagatcaggt
     2041 atatacagat aaaattgaca ttacccctga agagatctat caacgtttgc cagaggaaat
     2101 ccctggtaca tctttgccgg atggtgagtc gatcacagaa atctttgagc aaatcaaaaa
     2161 agacggttat gaaaaggtac ttgcggtaac aatttccagt ggattgagcg ggacatataa
     2221 tgtggttcgt ttaattgcac aacagcaaga agaattggaa gcatatgtac tagatacgaa
     2281 aaatattggt attggtgcag gtttttctgc gatccaagca gcaaaatggc tggaagaagg
     2341 agtagaatgg gatcaactga tcagtaatct gaatgagtta gtcgaaagaa caaaagtttt
     2401 ctttaatgtt gcgacattgg aatatcttca aaaaggtgga cggatcggtt tagttgcttc
     2461 tattgtagga accgctttaa agcttaaccc aatcatctca tgtaatgaag aagggattta
     2521 ttacacagta ggaaaagcta ggggacgtaa aaaaagtttg gataaaacgg ttgcttacgt
     2581 caaagaaaaa atcggtaatg caaaggtctt caacttagct gttgcccatg gggatgcgaa
     2641 agaagaagca attgaaatgg aagcgagact taaaagagag ttcccacaag caaaccaaat
     2701 ctactttggc cagatttctc cggcattagt agtccacact ggtccgggat tattgggtgt
     2761 cggtgtacaa gttttagaag gataaatcaa aaaaaggaaa cactgaggtg aaaatcagtg
     2821 ttttcctttt ttattgaagt tagaacaaaa aataataaag tattttgcac tttcatttta
     2881 attttctctt ttgttttata ggcaaagatg aaacaaaagc agaaatacgg tataatgaga
     2941 aggtatgaca ataatatata agaggtggct ctttatgaaa aaagttttag ttgtcgatga
     3001 tgagaagccg atttcggata tcgtcaaatt caatttagcc aaagaaggct atgacgttta
     3061 tactgcatat gatggggaag aagccttgga aaaagttgct gaggtagaac ctgatttgat
     3121 tttacttgat ttgatgctac ctaaaatgga cggcttagaa gttgctcgtg aggtacgcaa
     3181 aacttatgat atgccgatca ttatggtaac agccaaagat tctgaaattg ataaagtcct
     3241 aggcttagag ttaggcgccg atgattacgt gaccaagcca ttttccaacc gagaattagt
     3301 tgcacgtgtc aaagcaaatc ttcgtcgcgg tgccacggct gcgaaggaac cagaagaggc
     3361 agcacctgcg gaattgacga ttggtgattt gacgatccat ccagaagctt acatggtgac
     3421 gaaacgagga gaaacaatcg agttaacaca tcgtgaattc gaactactct tttacttggc
     3481 gaagcacctt ggacaagtga tgacaagaga acatttatta caaacggttt ggggttatga
     3541 ttattttgga gatgtacgaa cagtggatgt aactgtacgt cgtttgcgtg aaaagatcga
     3601 agataatcca agccatccaa attatttagt gacacgtcgt ggtgtcggtt actatttgcg
     3661 taacccagaa caggagtaat tggatgaaaa gaaaagtccg cttttttcga tcagtcaatt
     3721 ttaagatcgc tattaccttt attctgattt tattgatctc gatcgaaatt attggtgcct
     3781 actttatacg tggtttagaa cgttccacga tcaatacatt tattaaagat atgaatcaaa
     3841 cggtggaatc attagcaaca acaatcagcc cggaattgaa tcgaaaagat aatgcagatg
     3901 atgaagaggt aaatgcgaat atcaaaagat ttattgaaaa cagtgcgact agcgatatca
     3961 tcgaaatacg ggtcgttgac gaaaaaggga tcatccgagg aacaaccgat gtcaatgaac
     4021 aaagcgctgt gggaaagaaa aatgattatg ttgatatcaa tgatttcacg actaaacgtt
     4081 atgttgcttt agataacgac gataaacggg tgaatatcaa tgttcaaccg atcctgtccc
     4141 cgaccggcga tgccgttatc ggggctcttt acgtcaaaag taatatcgaa caaaaatatt
     4201 cggaaatcaa taacacagca gtcattttct ttactgcctc actaattgcg gcatttattt
     4261 caatgatcgt atctgtttta gttgctcgtt cgattacaca gccgatcggt gaaatgagag
     4321 aacaagccat tcgaatcgct agaggagact acagtcggaa agtcaaagtg catggacaag
     4381 atgaacttgg acagctggct gataccttca atcagttagc tgaacgtatt gaagaagctc
     4441 aagatacaat ggaagctgaa cgcaatcgtt tggatagcgt gctatcccat atgacagacg
     4501 gcgtcattgc gacagatcgc aggggtaaag tcattacgat caatgatatg gctgtttcct
     4561 tgctagatat caaaaacgag gaagcaatcg gacaatcgat tttgacactt ttagatatcg
     4621 aagaagaata tacgttgcgg aaattattag aagatccaaa cgaaatggta cttgaccgtt
     4681 caaccagtac attggaaagt gatcagatga tcttgcgagt agacttttct atgatccgtc
     4741 gcgaatcagg ttttatcagc ggattagtag ctgtcttgca tgatgtgaca gaacaagaaa
     4801 aaacagaacg ggaacgtcgt gaattcgtct ctaatgtttc ccatgaactt cgtacaccgt
     4861 tgacaagtat gcgtagttat atcgaagcat tgagcgaagg tgcgtggaaa gacgaggaag
     4921 tagcaccaaa ttttttgaag gtaacgctag atgaaacaga ccgaatgatt cgaatgatca
     4981 atgacctctt aaatctatct cgcatggata caggtaatac ccagcttcaa ttagaatatg
     5041 tcaatttcaa cgagttagtg aattttgttc ttgaccggtt tgatatgatg gtgggaaatc
     5101 aggaaaaaaa ttataagatc cgtcgtgaat ttacgcaaag agatctttgg gtcgaagtag
     5161 atacagataa aatcatccaa gtagtcgata atatcatgaa taacgcgatc aagtattcac
     5221 cagatggggg aacgattact tgtcgcttat tagaaacgca taataacgtg attttaagta
     5281 tcaccgatca aggattgggg attcctaaaa aagatctgaa ccgagtgttc gagcggttct
     5341 atcgagtaga taaagcacgt gcaagagcac aaggtgggac aggacttgga ttagctattt
     5401 ctagagaagt gattaaggcg catcgaggcg ctatttgggc agagagcaag gaaggaaaag
     5461 ggtcgacttt ttatatctcg ctgccatatg aaccttatga ggaggaatgg tgggaatgag
     5521 aatctctgaa aaagtcgttc gtataggact cattttgatg gttgcgttaa gcatatactt
     5581 ctcttatgca atctggctga gcccagcagg aaaaagttca atcaacgtag atgagacaaa
     5641 cactcaaatg atcgagtctc aaagcaatcg aaaaacttcc gaagtctttc ttccgcttca
     5701 tgttacatgg caccattcgg gaatgatcca agaaaccaac agtgaaaatc tcgtgtaccg
     5761 attgcaattg atcatcgata aagcaagatt tggaaagctt tctgaagtag tttcaggaga
     5821 caaatcacag tttgaacagg tgaaaaatct gaactctggt tttgagttgg cctataatgc
     5881 gccttttttg ctaagagaat acaaggaagc atttgattta gatttagatg tgtcgaactt
     5941 ggcaaaagaa agcgaaaata tgtattttac tagaatacaa ttcgatcaag gaaataaaaa
     6001 aatccgattt ttgaattatt cgaaccatct cgtatatgaa gcaaatattt cgttgaattg
     6061 gacagatatc aataaagaat taacagcaaa agacacaaaa tggacagaaa tgacaacaga
     6121 accatcgacg atcgacacgc aatatgctac gaaacattcg atcaaactga agaagtatag
     6181 ttatatcctg tcacagcaac cctatacact ttttagaaat gcttttttcc agaaaccaga
     6241 tagcgtgaaa aataatgatg aaagcaatga attgattttt tacggtggga atgaaacaat
     6301 gtctatccat tcggaaacgc aagtagttga ttttcgtgga gagctgccag agaaaaaaga
     6361 aaatgctagt gtattttctg aaagtttccc ctatattagc aaattaggca gttctttagg
     6421 gaacctcaga tattttgacc gcagtgacaa tacgatcgat tatcggatat tcgtggaagg
     6481 cttcccggtt tttgggacag acagcagagg acagattggt gtggaagttg gcggtgaaac
     6541 gaatggttct gtgcaagtga atatccaaac cagtttgaat acgattcaag tgccgatacc
     6601 ttcagacgaa gaagtagaac ttgcaagtac gaatgaaatc atccagcagt taaaagctaa
     6661 gggagcagat agcaacaaga ttggttcagt gatcgttggc tatacatggc aaaatgtcaa
     6721 agaaacgaat cgtgtcgttg atctcttacc tgaatggtat gtgaaatata aagataactg
     6781 gtattcagct aacgaccttc tggaaaaatt acctggattg gaggcgaatt aattggattt
     6841 caaaaaaatc gaatggatat ttttcgtcgc ttttttaggg ttgaatgtgt ttttattcag
     6901 cagttatcat gacgcggtat accaagaaaa caatgtcatt cgctctaatc agactgaatc
     6961 aatccaacaa cgtctggcta gcgacgatat cacttacacg gggaagattt cgtctgagaa
     7021 caagcaaggc tattatctta gcgcggagca gaccaatctt agcacttcat tagcgaatta
     7081 tcgcaaggct ggtaaaagcg gcttgctggc aagtggtgtg tttttagacg gtgatgtttt
     7141 gacgaaatct ttatccagtt cagatgctga aaagaacgtg atcgatccag ataaaattag
     7201 tagttccttg gaagattttt tatctaacaa agacaatatt ttattcggta atgattatgt
     7261 gtacacgaaa aacttttctc atttagaaga agaaccacta gaaatccaag caagccaaga
     7321 gtatgaaggc atcccattca atgatgatac agcaaaaatc gttctttctt tagaaaaaaa
     7381 cgataatgat tatagcatca cgaaatacat gcagacgcat ttatctgata tcgagcagct
     7441 gcgcgaaaag acggaactgt atacggaaga ggaagcaatc aatacattgt acatcaataa
     7501 caaaatacca agaggatcaa aaattttatg gcgtcagcta gcttattcct gtattctgaa
     7561 agtccgggaa aaaaatgtgt atgttcctgt ttggcatgta gcgatcgaaa caagcgataa
     7621 agtggtccaa attgagagcg tcaacgcttt cagcaatacg atcgtgacaa acaatactgt
     7681 accaaaagtg gaagatcaat aaaaaagcga gtataatgaa aaaagtttac aaagtaagaa
     7741 gggaagtagg agtcaaatgg ctgaagaaaa accagctttt aaaatcagta tcctagcaag
     7801 tggaagttcg ggtaattcac tttatatcga aagtgaaaaa aaacggcttt tggtcgatgc
     7861 aggacttagc ggtaaaaaaa tcacttcctt gatggctcaa attgatcgga caccggacca
     7921 attagatgga atacttgtga cgcatgaaca tcgagatcat atacatggcg tgggtgtctt
     7981 ggctcgtaag tatcatttgg atgtttttgc caatcagcag acttgggaag cgatggaacc
     8041 actgattgga aatgtaccag tggaacaaaa acatctattt gagatgggga aagtcagaac
     8101 attcggcgat atcgatattg agagtttcgg cgtctcccat gacgcagcgg ctccgcaatt
     8161 ttatcgtttc cataaagaaa atcggtcatt cgtcatgttg acggacaccg gatattgcag
     8221 tgattatatg cgaggtgtca ttgaaaatgc cgatggttat ttgatggaaa gtaatcatga
     8281 tcttgagatg ttacgcatgg gaccttatcc gtggaattta aaacagcgta ttttaggaga
     8341 tagaggacat ctttccaacg aagacggtgc acttgtcatg actgatgtga tcggtaatcg
     8401 gaccaaacga atctatcttg gccatcttag caaggaaaac aatatgaaag aacttgctca
     8461 tttaacaatg gaaaatgttt tgaaagaaaa agattttggc gttggacatg attttgaaat
     8521 ctatgataca gatccagatg aagcaactga gctattttcg atttaaacag ttcaatcaat
     8581 aaaaatataa aaagcagaag ataaaatcaa tcagggttgg ttttatcttc tatttttatt
     8641 tttttagcat attaaaaaaa taaccgtatt atcgactgac tacactgatg tgctgctttt
     8701 catgtttctc atccagtatc tcatctacta atgcaatcgc gtaatctgcc atgctgatcg
     8761 tactgtcgct agcagcgttt ctttgtaaat aatcatcact taacaaataa gtacctgtac
     8821 gtttgccatg tggatcaaat aaagaagctg gactgatata agtccaatgt agatttttag
     8881 attgtttcaa ttttaacaat ccttcgtaca tattgaaagc tgttggatag aagggtgcat
     8941 ttttaggtac ttggtcaatc atacgagtcg tcttttctgt atctaagaat aacgaagagg
     9001 cacccccaac gacaattaag cgtgtatcgg ttccttcaag catctcaatc aaatgggaca
     9061 aagaagtttg atgcatttct tcttttcctt tgggagcgtt gaaagcatca acgacaacat
     9121 caaaagatgc aagatcagta gttgtcaact caaataaatc cttttgtata aacggaacat
     9181 ctgatggcaa tttttctgga tgacgcacga ttgccgtcac gtctaaatct ctggacaatg
     9241 cttcgtctaa aataaatgaa cctgcatggc cagtagctcc aatgagtgca attttcataa
     9301 aaaacctcct tttttattcc tgtccattgt aagcagttgc tgaatattcg tcaaagaatc
     9361 cgatttttga aaagctcagt gcgcattcct ttcatggttc gtgatcattg ggtatagtta
     9421 aattaagaaa actgattgat aaaaatagaa gttgaaggag ataagaaatg aaagagtggt
     9481 tagatcgtgc ctttttacca atgataacat ctgttgtgaa tcttagaccc ttaactgctt
     9541 tgcgtaatgg aatgacttac acgatcccaa taattattgt tggctcgctg tttttcttat
     9601 ttgctcattt tcctgtggat tacatcgctc agtggataga caaaacagga ttttctatgt
     9661 attttacaca agtataccag gctacatttg atgtgatagc cttttggatc gtttttgcca
     9721 ttgcatacgc ttatgcaaaa gaagaagaca tcgatcgctt accagtcggc ataacagcct
     9781 tgtctgctta tctactgctt attcagccac taaatactca gatagatact gtctggacag
     9841 gtgctgaagg gatactagga gcagttacaa ctgggctcat catcggatgg ggctatgcac
     9901 gtttagtaag acttgcgaaa gggtggaagc aaagagagaa gatacctgaa aatgtttggc
     9961 agtcttttac ttcattgatt ccaatcattg ttatagtcac ttttacgctt atcggttcag
    10021 cggtttttca acatttgttc agaatgactg ttgttcaagg agtatggaca tatgtccagc
    10081 ttcccattca atcgttcata gacacgttgt ttggtgtgct tttattaggt ttttttgttc
    10141 cgtttttatg gttatttggt cttcatggat ctgcgatggt caatgggttg gttagtcccg
    10201 tcttgcaagc gaattcatta gcaaatgcag aaatcttagc ctcaggacaa gagttgacgg
    10261 tggcaaatgg cggacatatc gttactcagc aatttctcga tcagttcatg acggttacgg
    10321 gtgctggatt gacgttaggg gctgtatttt ttatgacggt ttttgcgaaa tcgaagaaat
    10381 acagagaatt aggaaatctt gctcttttac cagccttttt caacatcaat gaatcaatta
    10441 ttttttccac accgatcgtg atgaacccga tgatggctgt tccttttatt ttcgcaccgg
    10501 tcgtttcagg attgattacc tattccgctt tatattttgg attcgttcct ttatttacag
    10561 cagtccaagt cccttggaca acaccgccga tcctctcggg tttcttagta ggcggagtgc
    10621 ctgcagcgat tttacaaggt atcgtgttgt caattagttt tttcatttat ttcccatttt
    10681 tgaaaaaaat agattctgca aactgcaaaa aggaaagaca gacaaaaaat gatggagcag
    10741 cagaaaagat gaacgactaa aaaacaaaaa tactcctgtc gctttagatt gggaaaatca
    10801 ccaatctagg atgacaggag tatttatagt catgtttata ctagcccttt ttggacagct
    10861 aattgaacaa gaacaactaa tgtgttcatt cctacgtgtg tgatcatcga tgtccagatc
    10921 cgaccagttg ctttgtactc taggctgaag aagaaaccta ggaaaaagta gattagcaga
    10981 tgaccatcat tatgagcaaa cgcaaacagc aaagagctga tcactgctcc gacccaaaaa
    11041 tttgaatatc gactgatgat ccccagtata ctgcggcgga atacgaactc ttccatgatg
    11101 ggtccagcaa cagttgtcgc caaaataaaa gcgggtgctt ccatgatcat ctggatgata
    11161 ttttgtgtat tctcggaagg ggttgctttg ccaaacaata cagattctaa catgacagcg
    11221 atgctttgca gaatcaatgc gacaaagatt ccaaccaatc cataaataac aatctttgga
    11281 gcagaagctt tttttaccgc tgattcgatc gctaagggtt cacgttgctt aaaataaaaa
    11341 atagctagga caacggcgcc tagtatatac atgactgcag ttgctgtgat caattcagac
    11401 gtagatttta caagtcctat cgaagctagg atcaagggtg agataaaaat tagaccatag
    11461 caaaaaatgc tgaggaagct atattttttt aaagacatgt aattcctcct tttaagggat
    11521 aacaagcatg caaggcaagc ttatatcgat tgatttcaat tatatcataa tttcaataag
    11581 cagctgtcag aataaaattt cttttctttg atttaaaagc ttgcaaaaaa gagggagaat
    11641 gagtattatt aaaaatgtag gttagcactc gaaggttaag agtgctaaca gattaaaaaa
    11701 agagttatcg gagggattta tcgtgttaaa accattaggt gatcgcgtca ttatcgaagt
    11761 cgcaaaagaa gaagaaaaaa ctgtcggagg aatcgtttta gcttctgctg ctaaagagaa
    11821 accccaaact gggacagttg ttgcagtcgg tgaaggccgt ttgttagaaa acggtgaaaa
    11881 agttcctgcc gctgtaaaag caggcgacca agttatgttc gaaaaatacg ccggtacaga
    11941 agtaaaatac gaaggaaaag aatatctgat cgtcgcagga aaagatatca tggcgattgt
    12001 tgagtaaatt tggaagataa aaaaataatc aaaaataatc aatgaggtga atcaatcatg
    12061 gcaaaagaat tgaaatttgc agaagacgca cgcgcagcta tgttacgcgg ggtagataaa
    12121 ttagcagata cagtgaaagt aacattagga cctaaaggcc gaaatgtcgt actagaaaaa
    12181 tcttacggct ctccattgat cacaaatgat ggtgtgacga tcgcaaaaga aatcgaatta
    12241 gaagatcatt ttgaaaacat gggtgcaaaa cttgtatcag aagtagcatc aaaaacaaat
    12301 gatattgcag gagacggtac aacgacagca actgttctga cacaagcaat cgttcgcgaa
    12361 ggtttaaaaa acgtaactgc aggtgctaat cctctaggca ttcgtcgcgg gatcgaattg
    12421 gcaacaaaag cagcagtcga agaattgcat aatatctcaa ctgttgttga ttcaaaagaa
    12481 gccattgcac aagtagcagc tgtttcttcg ggttctgata aagttggaca tttgattgct
    12541 gatgcaatgg aaaaagttgg aaacgacggt gtcatcacaa tcgaagaatc aaaagggatc
    12601 gaaacagaac tagatgttgt agaaggtatg cagtttgatc gtggttactt atcacaatat
    12661 atggtaacag ataacgacaa aatggaagct gttctagaaa acccatatat cttgatcaca
    12721 gacaaaaaaa tctctaacat ccaagatatt ctgccattgt tagaacaaat cttgcaacaa
    12781 tcacgtccat tgttgattat tgctgacgat gtagatggtg aagcattgcc aactcttgta
    12841 ttgaacaaaa tccgtggaac attcaacgta gtagctgtaa aagctcctgg tttcggcgat
    12901 cgtcgtaaag caatgcttga agatatcgct attttgacag gtggtacagt aattactgac
    12961 gatctaggtt tagaattgaa agatgcaaca atcgaaaact taggaaatgc ttctaaagtt
    13021 gttgtagata aagacaatac aacgatcgta gaaggttctg gagaaaaaga agcaatcgaa
    13081 gctcgtgtgc aattgatcaa aaaccaaatt gctgaaacaa cttctgattt tgaccgcgaa
    13141 aaattacaag aacgcttagc taaattagca ggtggtgtag ctgttgttaa agttggtgca
    13201 gctactgaaa cagaattgaa agaattaaaa ttacgcattg aagatgcatt gaatgctaca
    13261 cgtgccgcag tagaagaagg aatggtctca ggtggtggta cagctctagt gaacgttatc
    13321 agcaaagttt ctgctgtaga agcagaagga gacgtggcaa caggtatcaa gatcgttgtt
    13381 cgtgctttag aagaaccaat tcgtcaaatc gctgaaaacg ctggttatga aggatcagtg
    13441 attgttgata aattgaagaa cgtggaacta ggcactggtt tcaatgctgc tactggagaa
    13501 tgggtaaaca tggttgaagc aggtatcgta gacccaacta aagtaacacg ttctgccttg
    13561 caaaatgccg cttccgtttc tgccttgtta ttaacgacag aagcagtcgt tgcagataaa
    13621 ccagaaccag cagcgccagc agctcctgca atggatccat caatgatggg cggtatgatg
    13681 tagtaattaa gaaagaaccc tattttaaca gggttctttc ttcttttttt gttttatttt
    13741 aagcaaaagg ggcaaagaag gggcaaaaat atcaaaggct atttagttta tcaattactt
    13801 gttgtttggc tctttttgtt acatggttat aaatagacag agttgtgttt gcatcagaat
    13861 gccctacacg ctccataatg gctttaagag gtacgcctaa ttcagataat agtgaaacgt
    13921 ggctatgtct gaatatatgt gaggatagac ttttttctaa ttccaactct tcctctactt
    13981 tatggagtat agcgttaaat gagtgcagcg caagcggagt gccacttgta gatataaata
    14041 tatattgatc gggatctgtg ggtctacctg caagaatatt gtcagctatc acactttcaa
    14101 tcaattcttt tgcacgattg ggtaattgca cttcacgttg cgaataagta ttttttggag
    14161 ttgtttttat agcattatcc attttcacag atgtgtaatc taaggtccca ttaatggaaa
    14221 ttttcccatc ctcatagtcc ttcatttgca aagctagcaa ttccccatat ctcaaaccag
    14281 ttaaatatag aaactcagct attatgccgt gtagttttct gcgaggattg gagtatagct
    14341 gtttcagtat ttgatcaatt tcttctttct ccagatattt tttattcata gaaagccttc
    14401 ttttttcttc ttccactttt ttaggatgga ttttaactgc tagcgcaggg tttctttgaa
    14461 tgtattttct atcgattgca tagtttagca taacagatag agttgttttt gtttgttttg
    14521 tgtagttcag tgagaggtca ccaaacgtat acatatcttc aattatctta ttaatgagtg
    14581 tctcatcaat gtttctaacg atcgtatcat cgcttatgtg cttagaaaca tgtttcatca
    14641 tcattggaac cttcaaatag ctagtacgtt taacatgctg cttataatat tcataccatt
    14701 ctttatacag ctcaccaaaa gtgatatctg atttattgta atcttcgagt gcttctttaa
    14761 tttttttatc tagaattttc tgagcttttt tccacgcttg tggtgaatta cttgtaagtg
    14821 ttgtagattt ttttcgtgtt ttttctgtat aaggatctac atatctttca ataaacttga
    14881 attggccatc tttggtctgt tcaacccaca cttttaacat ctcctatcat ttgctataat
    14941 aggcataaca aatagaccta tataggtttg ttttctaaaa gcacgctctt actctggacg
    15001 gtggggcgtg ttttttattt attatacagt tgctttaaaa atagctgctt tttcaaatga
    15061 tactaaagga gagaagtgga tttctatttc tcccttttgg tttaacgtga aatgagcagt
    15121 tacatccatt tgttttcctg gagcaacaga tcccattgta ttttcgttgg catatgtctc
    15181 tgattttttg tcatctggtc catatacttc cacatctgta cctacaggga tatctgaatc
    15241 gccatcattt tttacggtat aagtaatttt tactacttgt gcaggttggt tttcttcaaa
    15301 ttgatttctt tcatcagtta gttctacact atttagtgta tattcagcat caccaacttt
    15361 tacagtgtca ccaatcttat agaaggtatc gcttttttct tctttagaag atgaagcaga
    15421 ggttgaagat ttagttactt tttcgccacc attatcactt gctttattac tatctgatcc
    15481 accatttaaa gcagaaccaa taattataat taaaattact gctaataccc aaaaccatac
    15541 ttttttgtaa aaaggtttac taactttata catttttccg tcttgaccca taactttttt
    15601 tgccatttaa atattcctca cttcttgtta taatatattt gcgatctcag aaatgaggta
    15661 tgagtccgtg tgccagcacg ggctttttta atgttttgct gaaatcggtt tttttaataa
    15721 ttcttgatgt attcatacat attgccttgc gtaagaatat tttttcttaa aatagcattg
    15781 gcagacagca taacaagtgt atctgagctt attctgaata gaataatact ccataaattt
    15841 ttcgagatta aactgagatt catcagtcag ttcgttctca atatagatat ttaataaaat
    15901 tagaatagct attttatctg cttctgtttc aaattttgag tgaaaagttg tagaagtatc
    15961 gtacaaaact gaatattcaa aatgagaagc ggtgaaatgc gcaagttcat gagataaatg
    16021 aaaggcttct gcagtttcgc catatagatt ttcattcaaa aaaatgattc taggcttcgg
    16081 ataatagaag ccaggctctt tcatttccat gtaaactact ttcaaattgt attcgctcaa
    16141 catttctttc aatttcaaat acatacaacc catcactcca actattcatt ttcctctaaa
    16201 gctttagcaa ttgcaatcgc tttacgcatt gtctccttag atatttcttt tccatcaaaa
    16261 gaaaaaacag tatcgttttc tgataaatcc acatgtttag gggtttctct ttcttctcta
    16321 cctagaaggt agtctacaga gactccaaag taatcagcta accgagctaa ctcaatagaa
    16381 ttaggagttt gtcttttcca acttcctaaa tatccattcg aatacccgaa attcatttcg
    16441 agctgtctaa tggataatcc tttttctttt gctaactcct ttattatttc gtaagtattc
    16501 attgataaat caacctttct gaatgcttac aaaaaaagtt tagataaata atgtaaaaat
    16561 agttgac
//
LOCUS       DAZULO010000060        16560 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000060 DAZULO010000000
VERSION     DAZULO010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.061"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..258)
                     /locus_tag="ACG8OA_001889"
     CDS             complement(<1..258)
                     /locus_tag="ACG8OA_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976659.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="ACG8OA_001890"
     CDS             complement(274..924)
                     /locus_tag="ACG8OA_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976660.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="ACG8OA_001891"
     CDS             complement(935..2107)
                     /locus_tag="ACG8OA_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFS4976661.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="ACG8OA_001892"
     CDS             complement(2126..2998)
                     /locus_tag="ACG8OA_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS4976662.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="ACG8OA_001893"
     CDS             complement(3020..3940)
                     /locus_tag="ACG8OA_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976663.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="ACG8OA_001894"
     CDS             complement(3940..4209)
                     /locus_tag="ACG8OA_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976664.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="ACG8OA_001895"
     CDS             complement(4206..4586)
                     /locus_tag="ACG8OA_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFS4976665.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5053..6516)
                     /locus_tag="ACG8OA_001896"
     CDS             complement(5053..6516)
                     /locus_tag="ACG8OA_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4976666.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6580..9597)
                     /locus_tag="ACG8OA_001897"
     CDS             complement(6580..9597)
                     /locus_tag="ACG8OA_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4976667.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9748..10587)
                     /locus_tag="ACG8OA_001898"
     CDS             complement(9748..10587)
                     /locus_tag="ACG8OA_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HFS4976668.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10732..10977
                     /locus_tag="ACG8OA_001899"
     CDS             10732..10977
                     /locus_tag="ACG8OA_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976669.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11040..11462
                     /locus_tag="ACG8OA_001900"
     CDS             11040..11462
                     /locus_tag="ACG8OA_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976670.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11556..11873)
                     /locus_tag="ACG8OA_001901"
     CDS             complement(11556..11873)
                     /locus_tag="ACG8OA_001901"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976671.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11900..12106)
                     /locus_tag="ACG8OA_001902"
     CDS             complement(11900..12106)
                     /locus_tag="ACG8OA_001902"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976672.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12087..12269)
                     /locus_tag="ACG8OA_001903"
     CDS             complement(12087..12269)
                     /locus_tag="ACG8OA_001903"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976673.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12283..12633)
                     /locus_tag="ACG8OA_001904"
     CDS             complement(12283..12633)
                     /locus_tag="ACG8OA_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976674.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12659..12961)
                     /locus_tag="ACG8OA_001905"
     CDS             complement(12659..12961)
                     /locus_tag="ACG8OA_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976675.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12964..13197)
                     /locus_tag="ACG8OA_001906"
     CDS             complement(12964..13197)
                     /locus_tag="ACG8OA_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976676.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14101..14631)
                     /locus_tag="ACG8OA_001907"
     CDS             complement(14101..14631)
                     /locus_tag="ACG8OA_001907"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976677.1"
                     /translation="MKMTPNHLRHICCEVTLFNRQRWKLSNRIVRRIIVDSTGKSTVI
                     LTKKLATKQDFQLFFSQVKTIFFDGKIIANRGCSNQDLMVSNIVFSRMKRRQDLLIQA
                     RKAYKVFKKQEKQSRGKQNEQIMNKKCIEVLNGKNDHSDTKKKEGRHENYPILLVCPN
                     CFSCGFVGRIGFRTWN"
     gene            complement(14628..15743)
                     /locus_tag="ACG8OA_001908"
     CDS             complement(14628..15743)
                     /locus_tag="ACG8OA_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS4976678.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(15749..16147)
                     /locus_tag="ACG8OA_001909"
     CDS             complement(15749..16147)
                     /locus_tag="ACG8OA_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS4976679.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         4399 a         3452 c         2577 g         6132 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctccaagcaa ttactgattt ttaaattctt tataaataaa ataaaaatcg
     4861 tttgagtaaa gaaataagtc aatgaagacc caaacgataa aaaatattcc taatactata
     4921 tttaataaac tacgttctag atagatacga gatttcaaat taaaaaacga aaatgagaga
     4981 tacaaatata tcaaatattt aaaaagtaac cacattttct ataccactcc ttatcagaaa
     5041 cagccttatt ctttagaata agactgtttc tgggttgatt aatttgtctt ttgttaaact
     5101 accatctaag gcaagttcta aatgtaaatg tggtcctgta ctatttcctg ttgaacccac
     5161 ataaccaata atttgtcctt gttcaactgt ttgtcctacg ttcacgcaat attcctgttg
     5221 gtgcgcataa agagccacac ttccgtcttc atgctcaatt gtgacataat ttcctgacaa
     5281 agtatgatac gaagcttcta tgaccgttcc tgccttgctc gcatagattg gttctccttg
     5341 attggctgcc atgtctaatc ctctatgaaa ttcttctcct cgccaaccga aggaactaga
     5401 aattgaataa ttttttagag gtaccatata gccttctttg cttagttgtg gaccagctac
     5461 ttctttatca tatttgatta agtcataggt ttcgattaac ccatttaatt ttttatcgta
     5521 gctagtgtct gttgcgtatc ttcctgttaa aaatttagtc gcttcctgat aggtttttgc
     5581 cttacttttc caaacaccat cataaaaaga ggtatttcca actaagcctt cttttaacag
     5641 tttggcatag tcttttaagc tttcttcata gctctcatat tgccgaaaag ttgcttgggt
     5701 cgtataaagt gtcccattgc ctaaatcttc ttgtgtcgca aaagagacgc cctttccgtt
     5761 atgtgtgcct ttgattccaa atagattgta gtttggtgcc tgagccaatt gactttgtcc
     5821 gcttgcggat tctaaaatgg cttgcgcgat cataacagaa gcgtataaat cattttcttg
     5881 tccaatttta cgagcagatt caccgatttt acggataaac gtttctacag acgcgtcttt
     5941 ttcaaaatga atagtccctt cgttatcttg tgtgctggaa gggactaatt cttgtatcgg
     6001 tgcgcttggt gcttggtggt agttttttgt ctcttctgaa ccgattgatt gtgcctgcgg
     6061 tacgtcattc ataggtacag tcacactctg ttcaggtgcc ttttgtgggc gttccgtttt
     6121 tgttggcggt tccgcttctg tttcggtact tggtttttga ggagtctgtg agctttcatc
     6181 agaaggttgc tcatttggtt cttctgacgg ttcctcctca ggttgttgat tgtcttctgg
     6241 tttgtctggt tgattcggtg tttctggttg ttcttcagaa cctgttggtg gagcgttcat
     6301 ttctggatta tctggtgtat ctattgtatc ttcagtcggt gtttctggtt gattttcttc
     6361 tccagtggaa tttgttgtcg tttcttcagt gatcgttgca gattgcgaat ttgtcagttg
     6421 attgttgcct tgttcattag ccaaaaccgc tggcgtatac ccaaatactt gacctactaa
     6481 taacaaagca gataaacatt tactcgtttt tttcacaaga ttgcctccat tcttcaatca
     6541 atcatctaga gacagctgat tgctgtctct agtcatactt taattgcttt gtttcatttt
     6601 acgacgaacc agaacagccc ccacaccaac aaaaagaaac agtcccaatg caattaacac
     6661 atcgttcact gtttctcctg tttttggtaa gctttgtttt ggtgtgctcg tcggcgtttg
     6721 tttgacaggg gttgtcggcg tttctttttt aatgcctttt gctgtcaatg tttgtttttc
     6781 attcttcaaa tcaaagtgac gtgcttcttc ctctttatgg tctttatcct tatagacaat
     6841 atgagttgcg accaatttct ccccgtcttt taacgtgcct gctttgtagt caaaggatac
     6901 tttaaactct tcagacgctt tagaaacttt acgtgcagtt tctaccgtac caacgactgt
     6961 atcttttcca tttttatcaa tcttatggaa ttgagtcacc cagtatttgg tttcccctac
     7021 cgtagattct ggaaattctt gcgatacatg atcgatcaac tcttggtctt tgtctggatc
     7081 aaatgtttgg tcgccattca ctgtaataaa gcgtgtttcg atttttggtt ctttcgcagt
     7141 cactgtttga tctttattgg ttaaatcaaa atgacgagcg aattcttctt cctcttcttt
     7201 gtcgttatag acaatgtgcg ttgcgacaat cgtgtcaccg tctgaaacca tgtctgcttt
     7261 gtagttgaac gtcacatcaa atgtttcttt ttcatcggtc acttttcgtg tttcttttac
     7321 cgtatctaat acagtgactt ttccatcggc agtaatttta tggaattgcg tcacccaata
     7381 tttctctgtc ccaaccgcag atttagggaa tacttgggac actttatcca ctaattcttg
     7441 gtcttttttc gggttgaaat cttttccacc tgtcgttgtc acaaacaaag tagaaatttc
     7501 tggttggtct tcttctggaa ccacgatttc ttcgtttttc acagtaattg gttgtacttc
     7561 tccgtctttg accgttacta aaatcgtttc tgtttgtcct gacgcttgcc cataatgctc
     7621 agggaacaca atttccgtaa atttatacgt accgtctggt aattttagtt tttctggttc
     7681 ttcttttgtt tcatacacaa aggacacatt tttattatgt gcagtcatac cttcaatcag
     7741 gaatttcgct cctggtaatt gtagatgatt gattgaatcg actttattga agtctacttc
     7801 acccgttttt aacaagttgt caatcacatc taattcaaac gtaagcacag gcgtcgtgtt
     7861 atcgccatag gccacagaga acgtggtttg ttcttcagaa ggttgacggt agttgtctgg
     7921 cacatttttt tcaatcaatt ggtatttgtt ttgatcgcca ttgtagaatt ttttgtttgc
     7981 cttccagtgt ccgtctttgt cagtttttac tgaatccaag acttctcctt ttttcacaag
     8041 gactgtttct ttatctggcg cagtaatatc tgttttggcg ataatatcaa attcgatatt
     8101 ttctgcagga acggtatcat aaacaggttt gccttgattc caggattctt ccgcattata
     8161 gacttctttg actttctttc cttctggaat gaactgttgt aagtcatttt ttgcagttaa
     8221 cgtcacttct ttgtttggtt caatcgtaat cgtatgagtt tgtttgtcta gaatataccc
     8281 agctggtgct ttgacttctt tgacatccaa tttattttga acatattttc cgtctttttc
     8341 atagaatgca gggatttctt ttaaggcgat ttcccctttg gcatccgttg tatattgttt
     8401 tgtttctccc gtatcatgac gtttgatttc atacgtcaca tttggaactt ttttccctgt
     8461 gacagcgtct agtttggtta gtttgaagtc gccatttttt agaactttaa tagttagtcc
     8521 agctctatta ggatctggca cataaagctt tcctagattt tgagcaacag gatctacata
     8581 ggcaaaactt gaacctaccc ctctagaatt tggatccagt gaagaaacag aaattcgtcc
     8641 gtcatttgag ttttgatttg cttttataaa caaactattt cctgattttc ttacttgaac
     8701 gttagctgtg ttttcaacaa ccgacccatc ccattgagaa aacacacctt ttgtatcctt
     8761 aacttctact tcttccccta ctttaaccgt cactgtttgg ctatcaaaag aaggatcttc
     8821 aagtaaattg tttatttttc tatctacagt ctgtttgaaa gcttcataat tagattgtcc
     8881 aatagttcca taattaacaa atgaaccacc taaaacttcc caaatatatg tttgtgctaa
     8941 tacatagtta tccattgaac cactagaata gtagccaaaa taaccaatta aagaaatctt
     9001 ttttctaata gcacttgtcg taattaaatt ttcgagtggt gtcacatcat attgaatacc
     9061 cgcactcgtg attgcgcctt cttgaatgca aaaaacatct tctccgtcaa tttgccagcg
     9121 aaccatgtaa tcatgagtat acattgttcc ttcaggagta attcgagtca tattcacatc
     9181 tttacgacca ttttcagtca atgttagatt ggtagcacgt gtctgtgcat tcttttccaa
     9241 gattgcttgc cattcttcag attcttgcgc caatcgttct tgttcagaaa aagaagtcgc
     9301 tttttgaata tcttcaatgg ttataaagta tttctcataa ccttcaggga cctcttgagt
     9361 cattacattg ggcggagaaa catcttctgt cgtgtcctgc gtagttgtct cttctgttgt
     9421 ttcacttgtg gtttcagtac tgatcgcttc ttcactactt tttgattctt cagtcttcat
     9481 tacttcttca gtagaatttg attcatttat cgtttctgca aatgccgtta cagatcctaa
     9541 catgggattg agaaatgtaa cgaaaattgc caaccaagtt gccattcttt taaacatatt
     9601 tttttcgttc ctttcatttt atttttcagt cttctaaagt aatttcttat ggacttcgac
     9661 accgaaacat ttttatcctc ctcaaaacaa aaaaagacta gaacttataa ttctagtctt
     9721 catcaaccac ttacttttct tttattttta gtagaaatct acagtgtgtt tttgtgaccc
     9781 gtcactgtaa ataataacat ccaccataaa acctttgttg taccactgcg aatttggatc
     9841 gctaacctct gcattagccc aatcataggc ttcatctaca gtgttaaata cctttccact
     9901 attaccaatc tctccaacta caattgttgg ctcttcaggt gtcgttgtgc caccatttcc
     9961 gccttgatcg cctccaccag gattgcttgg ttcttcaggc gttgttgttc ctccacctgt
    10021 gcttgggtta gaaggagttg tttctcctcc agtatttcca ccattacctg gttgtgtagg
    10081 tgtttgagta gtgccaccat tgttttctgg tgtacttggc gttgttgtac caccagttgt
    10141 tcctgtttga tctccagctg gtgttgttgg tgtcgttgta tttggtgtcg tttgtgtatt
    10201 tgaagtcgtt ccttgtccta ctggttgatt cggatcaact ttgtctttct cttgaatcac
    10261 tgtttgagcc acagtttcac ctgcgctgtt ttgtacactt gctacattgc cattaaatac
    10321 taatttattt cctgcataga ttaaatccac atttgtaatc ttattcatgt ccgctaattt
    10381 ttggacagta atatttgttg cttgactgat tgcgcttagg gtatctcccc agacaattgt
    10441 gtattcattc cctttaattt ctgctttgat ttgttccgcc gaacgaggtg tccaaacttt
    10501 gttttctgct gcttcagcat tcgttcccac agcaagacta actcctccga ctacgattcc
    10561 tagtgccaat actttcaaca ttttcattgt ttttcgcctc cgatttgaat aaatttattt
    10621 acaaaaacat agtaccaaac ttatgtctat cagtcgatat attttttgta gattttttta
    10681 ttttttgata tactaacttt ggtactaaaa taactaaaag gaatgtcaca catgcaactt
    10741 aataatttaa atgaaataca tgattgggct caaaataatt tacttaatcg taaacaagct
    10801 gccaaaatca ctggacaaaa ctatgatgct ttttctagct ctttacgtag aaataatatc
    10861 aaaccatttc tagaaatagg cgacgaacct ggtccttcca tcattcgctt atatctaaga
    10921 gaagatattg aaaaatacgc aaaacagctc gaagaaaaaa gacagaaacg tcaatgaggc
    10981 gttttctgtc ttggatttac aagtaaaaca ataataaaca cttttacaac ggaggttcta
    11041 tgaataagaa atacattgtt tcagttggtt caatcgtact gattgtactc gctattattg
    11101 ctttttctgt atccaacaaa acagaaacac agccacaaaa tacggttcaa tcgactacta
    11161 caagtcaatc tagcgatcat tccgtctatc cttcactaaa tagtttaaaa gaaacgcatg
    11221 gacaagattt aattgatctt ggttcttctc aaattactac tgtcttattt gaaaagaaaa
    11281 atattgcgtt tattactcct aaagaaggaa ctgaccttga attttctatc aaagtaacaa
    11341 ataccaaaga aaatttattc tccattcctg acattcaata tacccctaac aagttaaaga
    11401 ttggggatac gtttggtttg gctaaagtaa atgaacaata ctttgcttac tcactgaaat
    11461 aagtttctaa tagcaagagg aaaaaccctc ttgttatttt tatcacataa tcattttata
    11521 aaaacgcttc gaaaatatca acatttttga agaaactatt tttctaattt ctcaatttct
    11581 tccaagggat tttcgctaat gacccataca ggcacagatt gctctcgagg ctccccttct
    11641 tctaaccacc aacctgcata atgaacttct tcctcctcag cattaccatg ataccaacca
    11701 acaaaggatt catctacaac gaatgaaatt tcttcttctg gatcaaataa ttcatttttc
    11761 catgcttcaa ttgcttgttc tttggtgtac ttttgtttgt taaatacacc acaaatttcg
    11821 tcatctacat aaaaaatttc aaaatcaaaa ttactcgttc cttttactgg catcttaact
    11881 tttcctcctt tgactcactt caatagatat accactcatg ttcattccat tgtttactag
    11941 ttaattggag ttttatctta gggctttgtg cactctttcc aaaaacttga gcgtcgtttt
    12001 tttcttctaa ccaaaaaagg taatttctta cattcggtac taaatgtttt tcctgcattt
    12061 cttcatgaaa aacatataat tctgcttcat ttttcaacca attcattatt cattccctca
    12121 ttcacttctt cataccgcca ttcagatagt gagatttctt tttgtttctc cacttgtcct
    12181 ataaaaatcg ctgtattcaa caagctgatc acacttaata ataaagcaat gaaactaatg
    12241 gttacactta ctctttcaca ttttgtcatt ttgtttcctt ctttactcgt ttgttatttc
    12301 ttgtaatagg tctaataact ccttcaccga aatttgcaaa tggttcgcaa aaatttcaat
    12361 aaaagcactc gcacacatta ccagaccttc aactgatata tcactgcgac aataaaattc
    12421 tggagaaaca ctgatttctg attctttctt acaattgaac gaaaaaacta tttttgaatc
    12481 aaattccggt aattcttcca caactatgtt ttgggattct accaagaaag ggagtgcctg
    12541 ttctaatgct tcttctggtg tttgagcaga tagaataatt gcttcttgtt cttttatttc
    12601 attgttctca aatactttat aacttttttt cattgttagt tcctctttca ttcctctctc
    12661 aattagctaa gccgttactg gtacgccatt ctctaaattt agtatcgcta atccttaatg
    12721 ctcgtcgaat atcaatcatt cgataaccta ttgcacgaag ttctttatat cttttagcag
    12781 ttagcgtctc taacgtcatt tcaggcaatg ggttcgaatc aaaaatagaa ttcgaaccga
    12841 ttatttgggc caattgttgt acttgtaaca gctcttctga acttaattca gtgattgctc
    12901 gattgcctat cattgcatac attttctgtc gaatatactt cttttcttgt ttagacaaca
    12961 tttctatcca ctcctatttt cattatcatt tgttacctcc acttcagtaa atccacaaat
    13021 aaatgaattc atattccaag taccattccc ttcaactaca acttttctcc tgtcctcttt
    13081 cggatattca agaatcagac cgtttaccaa tatgtttttc actactacta atttgtttgt
    13141 ataccgtgga attgctgttc cgatatactt cactttattt cctacgctaa ttgtcatttc
    13201 ctcactccta ttctttttta tccaaaactt tttcctgtcc tattcctgcc aacgaatata
    13261 cttcactgca tgcttaaaca aaacaaaatg ttgccctgtt ttatttccgt cttttacttg
    13321 ttccacatac agttcatatt tggtcacacc ttttaaaacg ccagatactt tttttcatcc
    13381 attgtcacaa ttagtttttt catcaatcga ttgatactgc actgctcatt tttcttgttc
    13441 atatagtatt cctttctttt tcaaaactag cctcttttgt ttactagttt tcatcaatca
    13501 tcaacgattt aacagatttt ttctttcgca gggtttctag cgtcagcaac cccaaactct
    13561 aaaaaagtgc ctttgctccc ttgaaaaacg agcggacgat tgtactcgtc atcatatgtt
    13621 tttcatacta cctgatccac gcaaataacc tttttttgtt ctgtgccaca cccatccata
    13681 cgtatcgctt gagattcacc atactttttt aggtggttta tccttcctaa gtccctgtta
    13741 gacacgctga ttaacttaga aagtcctgtt tgtatccaat tttcaaagat ctcccaaaag
    13801 aaataagtag gtttacgtgt atcgcttctg ttcaaagtac ctgtttgtac ttgagtattt
    13861 ctactcccct cgttcaaggg attccttcca ccagctagct tttcctttca tcatcaaatg
    13921 ttcctgaaaa gcatttacgt ttcgtgtccg tgtgcttact tattcttttt tcgatagcga
    13981 ttatccgctc gctactcgct attttatact ttgtttgtta gctcaaataa aaactaacaa
    14041 acaaaaaagg ctagtttctt tccgttttat ttttttaact gtcgataaat agttgttagt
    14101 ttaattccaa gttctaaaac caatacgccc aacaaaacca caggaaaaac aattggggca
    14161 aacaagtaaa atcggatagt tttcatgtct tccctctttc tttttggtgt cgctgtggtc
    14221 gttcttgccg ttcaatactt cgatacattt tttattcatt atttgctcgt tttgttttcc
    14281 tctggactgc ttttcttgct ttttgaatac tttatacgct tttcgtgcct gaattaatag
    14341 gtcttgtcgg cgtttcattc gtgagaaaac aatatttgaa accatcaaat cctggttcga
    14401 acaaccacga ttcgcaataa ttttcccatc aaaaaaaata gttttcactt gagaaaaaaa
    14461 cagttggaaa tcttgttttg ttgctaattt ttttgtcaaa atcactgtgc ttttccctgt
    14521 tgaatctaca atgattcttc gaacaatccg attcgataac ttccaacgtt gccgattaaa
    14581 caatgttact tcacaacaaa tatgacgtaa gtgattaggg gtcattttca tgtgtccaat
    14641 tcctccaacc aattatgcat cgaaattttg ggcaattgtg acagcccata aatctgcgtt
    14701 tccaggtctt cataactaca agaacgcttg gcttgaagta atgaatattc tgcttgatct
    14761 gatagttttg ttgccattgg atctagattc aattctctta cacaacattc ccaaaaattc
    14821 aatagcgctc taaaataaaa tccttttcga ttctgaattg cttttccttc atgacgagca
    14881 attttttcat tttgaataca ccgttgatac gttttgctta aatcttctgc aaatgtttcc
    14941 cctaaaattc gatactcgct tccatcagat aaaccatgtg tttgattata ctttcgttgc
    15001 cgcttttcta taattcgttt actgtcgtat atcatgttta gctgctcttt cgcttcacgc
    15061 cattcagctg aactgcgaac aatccatgtc aaaacatctt tcggaacaaa cgtactttcg
    15121 ctgtaatgag caataaactc tttttgccaa tcatcagcca tttcaacagg actagtcgta
    15181 tcgatttttg tatctagatt ttctgtatct agattgttag aagaattatt aaataattgt
    15241 gcagaatttt cacatggatt tttttcgtca aaatcatcga aaatcgctaa attatcaaca
    15301 gattcctcct cttcttttcc ttgtgcagaa tctacactgg ttgagtcaac aggacctaat
    15361 gaatacacat cattttctgt cgtatctaat tctcctagat acaaccgagc tttgtttctt
    15421 tttcctgttt gttgatcaaa tcccatatcc tctatttcta gtagctcata ttctttcaat
    15481 tcttttttga ttgtattaac agaacgtctg caacaattca atgcattggc taattcttgt
    15541 tccgtaaatt caaaataaac aaaaccttga ctatctactt gatttcgttg aatggctaaa
    15601 tctaagcgtg accgaaaaac catatacgcg atttttgctt tatcagacaa tgagcgatat
    15661 ttttcacttg taataaatac ctttggaaat tgatagtata attcattgta tttacgagtt
    15721 gcggtaaatc tttttgttgc catcgttatt acacctcatc ttgtccatat attaagaaag
    15781 aattttggtg ttggtagaca cgaacatctt ccattgtagc aatcaattct tttagataaa
    15841 ttcgaccgaa ttcaattgtt agtaagtcgt aatcttctaa ttcatttttg atttcttcaa
    15901 tttcttcttc agacacattg agtaaaatgg ctaattcttg attggtataa gaaatataga
    15961 catgtttctt ttcatcaatc cgtttttcaa ttaaggattc ttctaagtac tcattcagta
    16021 agctataggc gtattttgct tgtgcagtca tcgcataata ttttgacgat tttataaaga
    16081 atagttttgg catatgaaaa gttgcttgtg tcagcacatc tgttgcgcga aatttgtttt
    16141 ttaccatatt ttttcctcct agaaaaacac gcaaaaaaag ctctgtataa atcgaattac
    16201 acagagtttc ttcttgttga tatttaattt ctaaaatacg tttcgggtaa aaactaaaca
    16261 cttactaata tttagattgt agaatcttcg ccttcattta ttcattttaa agagaaaaag
    16321 atcaagtgaa atgtgcccaa aagtttgatt ctattaattt ttaaacacca ttcataatat
    16381 atttcctata atgtaaaaag cgtcgatact tattaaagca tcgacgttat ttttatagta
    16441 aatttatcaa aatttttgcc aacatttttt ccgatttttt tccctatttc tatctttcct
    16501 tatctttctt agttaatatt atttgatgtt aatatatcac tactaatatt aaaaaacatt
//
LOCUS       DAZULO010000061        16492 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000061 DAZULO010000000
VERSION     DAZULO010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.062"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1452
                     /locus_tag="ACG8OA_001910"
     CDS             <1..1452
                     /locus_tag="ACG8OA_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4976680.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1801..2433
                     /locus_tag="ACG8OA_001911"
     CDS             1801..2433
                     /locus_tag="ACG8OA_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFS4976681.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3021..4460
                     /locus_tag="ACG8OA_001912"
     CDS             3021..4460
                     /locus_tag="ACG8OA_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4976682.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4755..4973
                     /locus_tag="ACG8OA_001913"
                     /pseudo
     CDS             4755..4973
                     /locus_tag="ACG8OA_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            5222..5437
                     /locus_tag="ACG8OA_001914"
     CDS             5222..5437
                     /locus_tag="ACG8OA_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976683.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5654..5910)
                     /locus_tag="ACG8OA_001915"
                     /pseudo
     CDS             complement(5654..5910)
                     /locus_tag="ACG8OA_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6097..7008)
                     /locus_tag="ACG8OA_001916"
     CDS             complement(6097..7008)
                     /locus_tag="ACG8OA_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4976684.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7027..8031)
                     /locus_tag="ACG8OA_001917"
     CDS             complement(7027..8031)
                     /locus_tag="ACG8OA_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFS4976685.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8028..10151)
                     /locus_tag="ACG8OA_001918"
     CDS             complement(8028..10151)
                     /locus_tag="ACG8OA_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS4976686.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10156..12603)
                     /locus_tag="ACG8OA_001919"
     CDS             complement(10156..12603)
                     /locus_tag="ACG8OA_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4976687.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12590..12979)
                     /locus_tag="ACG8OA_001920"
     CDS             complement(12590..12979)
                     /locus_tag="ACG8OA_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4976688.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13039..13542)
                     /locus_tag="ACG8OA_001921"
     CDS             complement(13039..13542)
                     /locus_tag="ACG8OA_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS4976689.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13555..13779)
                     /locus_tag="ACG8OA_001922"
     CDS             complement(13555..13779)
                     /locus_tag="ACG8OA_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976690.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13883..15793)
                     /gene="ltrA"
                     /locus_tag="ACG8OA_001923"
     CDS             complement(13883..15793)
                     /gene="ltrA"
                     /locus_tag="ACG8OA_001923"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS4976691.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         4913 a         3271 c         2939 g         5369 t
ORIGIN      
        1 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
       61 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
      121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
      181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
      241 agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact
      301 tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa
      361 tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac
      421 catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg
      481 acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat
      541 gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag
      601 tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg
      661 atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt
      721 gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt
      781 aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc
      841 tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca
      901 ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg
      961 tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta
     1021 accaactatg ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct
     1081 attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga
     1141 cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt
     1201 aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca
     1261 accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa
     1321 ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg
     1381 gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta
     1441 ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta
     1501 gacctttgat caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa
     1561 taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg
     1621 agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt
     1681 cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt
     1741 ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca
     1801 gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa
     1861 aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta
     1921 tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat
     1981 ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa
     2041 ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag
     2101 cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt
     2161 gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg
     2221 aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac
     2281 ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt
     2341 gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt
     2401 gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaag
     2461 aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag aaaagatgct
     2521 gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta attattgtgg
     2581 ataataatta acagtgatat ataaacatct ataatatatt attcatacta ttgatattta
     2641 gtataatcct atggaattga atatcatagc aatgtatttg aaaagcaaat tggaaaacaa
     2701 ataggtgcaa caccataaat atgaaaaaat tgggaaaaag tagaatggaa atttgcttac
     2761 gtctcacgag atagctcaca ctattaatct gaagaacaat gaagtcgact tttagaaatt
     2821 gctagaagaa agtaaagcga attatcacta gttactatag agtaagttca aataaagcat
     2881 ctccaactta catggttaca tgaggatatc aaatcgaaat tactcaagct tcctatggct
     2941 agagtcgcga gaagttgttg ggcagtagac ataggttata ttgttcttag ctacacgatc
     3001 attagaaagg aaatcctatt atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc
     3061 gctggtggat tttgattgct atatctatgt ttaccttcat gtccacattg gatggcagta
     3121 ttgtcaatat tgcactacct accatctcag atgatatggg cataccaatg aatcaagcgg
     3181 aatgggtagt gtccgtttat ctgatgattg tctgttcttg tttgttgctg tttggtaaaa
     3241 ttggcgatag ccgtgggaaa atcaaagtat ttcggattgg tacgctgatc tttgtcttgg
     3301 gatcgctgtt gtgtgggttc aatcattcgc taggtatttt attgttcgca cgggtggtac
     3361 aagccattgg tgctagtatg actatggcta caaatgccgg aatcatcacg gaagtctttc
     3421 ccatacatga acgtggtcgg gcattgggag caataggtgc gtttgtttca ttaggctcga
     3481 ttgctggacc aggtcttggc ggcttgattt tgtcccaatt ttcttgggcg tacatttttt
     3541 ggatcaatgt accggtggga atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag
     3601 atatcaccat gagcggccat acaatcgatg gagggggctt tgctgccatt gcggctacga
     3661 ttatcagctt ttttggagca atctttttgg ggcaagaaag tggctttttc catccagtcc
     3721 cccttgtact gttcgtaatt tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga
     3781 aagcagcctc tccattgatt tctttcggta tttttaagaa caagattttt accatgagct
     3841 tgattacagc cttgttgatt ttttcttcca acttctttgt caatgtagtg attccttttt
     3901 atctgcaaaa tgctcgcggg ttgagtccta gctatgctgg gctgttgatg atggtctttc
     3961 ctttgatgat ggtcgtcggg gcgcctttaa gcggctacct gaccgaccgg gttggcccgg
     4021 agattttggt attagtaggc ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt
     4081 tgtcagtgga ctctcctttg tggctataca ttgtcgctac tgccatcatg ggtgccggca
     4141 atgccttgtt ccagtccccc aataatacga tggttatgag ctcggtcact atcgaaaact
     4201 tgggactggc aggctcgatg aattcctttg cccgaaattt ggggatggtc agcgggattt
     4261 cgctttctac gacggtctta tatcaagcga tgtctgctaa gatgggccag caagtaacta
     4321 actatatcgc aaatcggccg gatgtctttt tatatggaat gaagattacc tttttaggca
     4381 gctttgtttt gtgttttgtt gcctttttcc tgactatctg gcgtatcaaa aaacaaggag
     4441 gtattcagaa aaatgtctga aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg
     4501 gtgagtggga tttccatgga tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc
     4561 aagtattatc ttcaaaatac tagtgctcaa tgagatgctt gatttgatca caagaagact
     4621 gcgaaagccg ctggcaaccg caagctaggt atcccaccga tggactacgt tttagaggct
     4681 actggttatg aatacggagc caacgctctc atctgcataa gaatacatca cccagattat
     4741 gtaaagattt tcgatacttc tgtcaaaaaa tcattaagat agataaatga tttccgtcta
     4801 gtcaaattgg ttcagaatgt agacacacag atggtaagaa gtcactcaca attcgagaat
     4861 ggacttgtcc tatttgccgt atacatcata atcgaaacgt caacgatagt atcaatattt
     4921 tgaccaaggg ctatgaatac aagcattgcc ttagaaaaca tcaagaacct taggaacaac
     4981 ggggatagct tggttaataa gagataccgc tgtttaggct tgcaagtata ctcttttatc
     5041 aagaattccg tggctttagt catgagaggc ttaatactat gaaaattttc tttagcattt
     5101 ttgatttagg ttccttttct aattataata agatgaccac tacagtaacc tactgaaata
     5161 tataaacagc cactgtagta ccattttcaa agtagtctat gagaggagaa tcttcattct
     5221 tatgaacgct ggaattgcgg atatgaattt aataaaaaaa acccttaatg attttacttc
     5281 caattcaata tcaaaaggta ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg
     5341 tgaaagatca gtggaaaagc tgaatctttt agatgctata aaaataacgg aatttgctat
     5401 gaaaaatgga aaagctgaga ttgaaatatg gagataatta tcgtctagtg aattacttgc
     5461 cacttagctg aatttaacga tccttggtgc ttgaaggggg agattcttgg gaatagagca
     5521 tacttgcaag cctatgtctt tactaacaac gatatctctt attaaccaag ctatccccgt
     5581 agttctaagg cttttacttt tatctaagca ataatcgtat tcaaaggaaa tttttgaccc
     5641 ttagaaagtc tcattttatc agacgtgagc ttaggcattg ctgaattttg ttaattcggt
     5701 tttaggttgt gctttgagca taatatgaaa gtattcttta tcacgatttc attcatctaa
     5761 ggtaatgtga tgtgattctg aaattctttc aaaggcagtt tttgcataag ctgacattac
     5821 atcatcaatg acatgtttac aatgtttcac tactaaaata agggggtaac caagaagaaa
     5881 aactgaatga ttatttgtat ctaattccat ggtatatcaa ccatttccta tatagactga
     5941 ttatgacatg gattaaaata aaaaggcact atgtcttaat tgtcggtttt cgagttaccg
     6001 aatggcattc atcacccacc tatagaggat gggcgacttc tgcctgaaat aagttacact
     6061 acttataact tctaataaaa ttagattgag tcattattat ttaacatcaa cgattttcca
     6121 gtttccattt ttttgtaata ctaatttgta ctgaaagtag ctagtggttt ttgatacatt
     6181 atcaaggtat tttatctttg tactcactag aaaatttttt ccgttttgta gataaactgg
     6241 attctctaac tccgaaaact ttaagtcaat attgattggt cgcatcgcac cattttcaac
     6301 ataatactcc aattcttttt cagaagctga tggataaagt ttaaaaaatg tttctaaaaa
     6361 atcggtgatt tcttccgttg ttttggcatc aatttctgaa tcgttttcta cttgttttgc
     6421 ttcatatttt gccttgtctg gctttccaat cattgttggc aaactagtta ctaaatgatt
     6481 cctgttttca tcttcaaaga ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt
     6541 tccctctgta atatgttgct ccactaaaaa tgaaacgaca aattcttccg agtgtttttc
     6601 aacatctaga atttttacag attgaacttc ggaactcgtg ggaatatctg ctcgaaccat
     6661 atcttggatt aaagcgagac cgttttctgt aaggtactgt tcgagattac tcattctctt
     6721 ttcaattgct tctttgttat ttttccatga aaaatattct ttcgcaaaat cctctgtaaa
     6781 actttcaacg ccagaagtat tcactacctt ctcttttata accacttttt catgagttgt
     6841 atgttgatca atggcggtga aatttttata aacgccaaaa gcaagactac aagacaataa
     6901 aagccaaagg gcaagcacca tttttcgatg cttacccacg gatacaactc gttctttttt
     6961 cttttttggg ctcttttcct tttgattccg ttcaattttt atcttcattc tttttcctct
     7021 tttctcttat ttgtatcggc cagctccgat aagatgctgc cgccaatagt tttccgttag
     7081 attcgtccac cccactggat ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc
     7141 cacatgggtc acataagtac cggcatcata ggttgaatga aagaaaacta aatcacctgg
     7201 tttggcatct tttagatcaa tatgagtcat ggcatcatat tgatcctgag ccacccgagg
     7261 aagtttcagg tcaactttgg cataacacca ttgggtcaag ccactacaat caaaccccgt
     7321 ttctggactt gagccaccaa acacataggc cgttccttca tacttaaatg cctcatcaaa
     7381 aatttcttgg actattttgt catcaaactg agttgtagtt aggtattgct tcactaataa
     7441 acagtaaaac atattgccat aattataccg ccacccacca ttttccttaa tcgcgattgg
     7501 gttggggtaa tcgacttttt gaccacctga tttttccttt gaaaaagatt cagctaaagc
     7561 aaaactatgc ttcttcccgt ttttcccgac atgatctaga tacccgccac catagttata
     7621 ggcctgaata atactatctt gatcacaccc tagttctttg gaacgcttaa ccaaagacgc
     7681 aaaatagaga cagccttgtt tgatggatgc ctcggtactt aatgaattag gtggtaatcc
     7741 ggctgactca gaactttgca taacgtcttc cattgtgcca ccagattcca cttgaataat
     7801 cgccaaaaga attggaatat actctgttat atccgattct ttggcatact tttccaccat
     7861 tgcacggtgt ttcaatactt cttctgagac actagttccg ccaggagaag tgccagaact
     7921 accatttccc tcactgtctt caccaaacaa caaacccaca cacatcaaaa tacccataaa
     7981 agagatggtc agaaaagtta atgaaataac acccactttc ttcaagttca ttttttctca
     8041 cctgaccttg attttggacg atggttcatt cttttttgta ctttttgatt ggtctgcttt
     8101 ggcagagtgg acctctgttt cacttgttgg aaagaatttt tcttacttgc tgattggctt
     8161 ttcccttttg aaatgatttc ttgactagtt agttttcgtt tgatttcggg attcagattt
     8221 gctctcacaa tccgctttgg tgcttctttt ttcagcatca tatctttctg aggttgagct
     8281 gctttatcta ttggaatatt cgcccttctt tttccctgac gatttgttgc ttcactcagt
     8341 atcttgcgtt tttcgtccat tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc
     8401 actcgatccg ctttttcttg gaacagtccg cgcttgaatt cttctggtgc ttttttcgac
     8461 ttttcgattc ctttatggag attgtatttc aaattcgttg gcgtattcct tacctgatcc
     8521 tttacttgtt tagtcttatc tttcaccaaa gcttgggtat ccaaaacttt gccgattttt
     8581 ctcccagcca aattcattcg ggattgtttc ttattatttg aaactggatt ctccttagtt
     8641 aagtcagata gtatttcgtg atcgttcggt aatcgtgaat tctttcgtag agaagaaccg
     8701 gatggcttaa atttggattt tgattgttcc tttgccaact tatttccgac tgctgcacca
     8761 ccaccaaaag ccaaagctcg tcccacattt cgattgagtt gccgcaattt tcgattcatt
     8821 aacatttgcg gttttcgcat gacgcgtctt cccattgact ggctatcatt actctggagg
     8881 ttgaacatac tcataatgtc gcccaatttg aaatagatac cggcaaaggt cacaatttgc
     8941 agaaaagcaa ccatgaaaaa tggataggtt gccgaaatat tgaaaaacat cgtggagatg
     9001 ctaaatgcag tcgtaatcac caaagtaaca cccgctcgca tcataatcgt attgaataaa
     9061 cgaataatcg ccttctttcc taaactttca taagtcggca acatggacaa taagaaacta
     9121 attggcagaa acatcgcaaa gataatgaag agaatttgcg agaacaacat aatccctgtt
     9181 agaagaaaaa caaagaagga aatgccaata ttgaacaata ccaaaaagac cacggtccct
     9241 aaacggttca tggtcttggt aatactgaga ttcgcattgt cattgtcttc aatctctgcc
     9301 ttgaccgctt cttcccgatc tttgcctttg ttttcatctg gactcacaga tagaatcttg
     9361 ttgacccggt cttcaccaat ttcttctatg ttggaatcat caaattgcag tagtaaccaa
     9421 ggttgttgga cttgaatcga aaacaaacta tcccgaatca aatccacact atcttttcct
     9481 tggctttctg aattgggtac aacgatctcg gttccaaggg taagggccgc ttcactaaca
     9541 tctgagctaa agtcattgat ttttgtgata taggtaggcg cataagcaat gaacgaaccc
     9601 gatagtagaa aaatcatcac aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt
     9661 tttaataatc cggtatacgc cacatacacc cccagagcta aaatcatcaa taatagaaac
     9721 ccaaaataaa atccggaggt ctggagtcca ttttcagtaa taccagccaa tgtttggata
     9781 ttctttccaa tactttcagc cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc
     9841 acataacccg tggcgttcga caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca
     9901 taaagaccat acatcacgct tttccctaag ccatcacccc aattccaagg caaccaatcc
     9961 caagagctat ccacaaagaa atctagttga tagttgttca gtgaatattt ggaatataga
    10021 tttcctgctt caactgtatc atcaactagc cctgtcgcct cggctaccgt accaactaaa
    10081 ctcaatagaa ctatcagagt gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt
    10141 ttctttttca tcccctcact ccattctctc ttgaacaggt ggacgggtat caaaggcatg
    10201 gaagagttct tcaaagactg gatgtacctg aaccacgcct acccgaccat acaaatcctg
    10261 catcaagcat tggccatttt ccaaatctcg taggcgtttt tgattgcttt catcttctcg
    10321 atccaagcca aagaattcca aggttttctt gatttcatga atatcggtac tgcgaaacgc
    10381 aaacttcaac ccgatattat tcttcaagcg ttcatccagt aaatcatcac tattttgcgt
    10441 gacaaagtaa accccggcat tcatagcccg cccagcacga acgagcttat tcgataatgt
    10501 tttcccttgc gctacttgaa gaaaactcca cgcctcatct aagtctacaa ttttgaagat
    10561 ttctcgatta ctatggataa agtccaaggc aaaagtggaa atgacgatta gcatggaaac
    10621 actaagcaat tccatggtgg tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa
    10681 atccgctact tgaatgatat ttaactgcct atctaaacta atcgactggg taatcgaacc
    10741 atctgagaaa agcaaatggg caaaatcata atcggtaaag gactcgatgt gatccgcgat
    10801 attttccgca actggtgagc cttcttttcg aagctcatta attacttgta ataatccccg
    10861 ttgattgctt tgggtaaccg tccgaatggc cttacgcaac actggaaact tctccccatc
    10921 tcgactagaa attcctgtta aaaaggtcag aatatcaatt gctagacttt cagaatcctt
    10981 ctttttctcc ataatcacat aaggatcaag taatccttga ttttttggct tactagttaa
    11041 gttgagaata ttgatttcat ccgcaatttc tggcaaggtt tctttccagc caccccgttc
    11101 cccttttgga tcgacaatca cagcttgtcc accaaaaagg acggcgtaat aaactaagag
    11161 attgttacta aaggactttc cgccaccgag tgaaccgagg aatgccgctg ctagcgcatt
    11221 ggtaactgaa tccttgaccc cttgtgaggc caatgccggt ttcaggtaaa cattgcgccc
    11281 cgtatctacg ttataaccaa agtaaatgcc ttccaactcc cctaacatct gagtggcgcc
    11341 aaaaccaagt ccggcgagaa aatcagacgt aacatactgg atataatcat tcatgtatcg
    11401 cttactagaa ggtaagaatt cttcatgtag ccccatcata tcgccaaaag gccggactag
    11461 ttttacattg gtatcatcat aaaaatctag cacttcatca caccggcgct ttagttcatc
    11521 tactgattcg gcacttactc gaaccacata gctcagtttg tacatggatt ctttggactg
    11581 atccaaggta gtttccaatt catctacgga atccaatgca tctaagacat tggaatttgt
    11641 ttcattgtca gattgatagg catggttgtc caagtctttt aattcctttt tcttattgcg
    11701 aacggtagca agtgcctttt tgttcgtcac aatctccaca ttcatactag tgtcaattgg
    11761 gaatgtaaat tgttgttgct ggtaatagaa aagttctgag gagggaaact ccatttcccc
    11821 cacaattgta ttaattgtta agtacgctac atatgattca tgattctcat gttccatgca
    11881 taagtaccgg ggcttttctt caatcaaaca acgactggga cgtaataagt cgtaacgttt
    11941 caccaaagtt tccgacttca gctttttctt tggtagctga aattcatatt cttcaaatgg
    12001 tgtacccttt tcgccataga tatgttcaat caaatacatc agatcactcg gtgtgactcg
    12061 tctgacttta aatcttcgag ccaatttact ttccattagt ttttcaagct tggtatatcg
    12121 gcgaatttct tcattggata aggaaacaaa atcgcccatc aaatgatgat tcactccata
    12181 gacaaattct tgaaagccgg agaagaaaga tttcttgaga ttctttagat tgacctcttc
    12241 atctgtcgca atcagtttga agccaataaa aaagcgataa tcaatctgac tatcgccaat
    12301 catggatact aatgcctctg tctgtaaatc aattcgttgt ttcgctactt ctgttaatcg
    12361 cccggtaatt tccttttttg atcgttcttg cgtggcacga atactacttt ctgtggcaat
    12421 ttgtaaggca tgaatcttgc cttcacgatt ttgcgcaatc aattgacgaa agttatcgtg
    12481 gacttgatat ttctgttcag gtgacaaaaa ggagtaattg taaggtatta actcatagta
    12541 ggcaaaacat tctccctctt gattaaagac aagattattt tcaatatact taatcgggta
    12601 catagttcac actccttacc agagtaatgg gttcgtccca tcgtttcttc tgttctttga
    12661 ctttctttcc tgcgaaagtg accttaaatc gtaagtgata gctaataaaa gatcgtaaaa
    12721 atcccaatgg tttcttaccg tcaaacgttt tttgcgacat gaaccaagta actgccaccg
    12781 gtatacctaa gtattttaaa aaggccccct ctatcataga tagtggaggc acatgggcaa
    12841 atacgatgac aaacagcaag gataaaacaa accaagccat ctggttaaaa gtcacaggaa
    12901 aaggcagttg aaagtcatta attgcataaa tgaccttttc aactgcccaa atactggtgt
    12961 agctttttat tttcttcatc aaatcattcc tttaaaaaga aggatagttt agaaatcaac
    13021 tacccttcaa taattttgtt aatatggata ctcaaaaaca cctcgactcg ttaccaaata
    13081 attgcctcca atctccaagt ctctaccaca cgcatcaaaa tcaatatacg tcttcaagtg
    13141 ttctggtact tctccaaaat caccagcttc tacaatcaag aaatatgcta catcactcat
    13201 atcatcgcaa tctggataat agataatatc atccacatgc tctaccatct cctcaaaaga
    13261 attgaagaaa gcatgttgaa tctcagaagc aacttcacca actggcgttc cttcaagttc
    13321 ctccgctaag ccacacaacc gattaatttc ctcaattggc gtatactcat caatctcaaa
    13381 gggaagttca taatcatgaa ttgcatattc ctcgtattcg tcatttaacc caattcgttc
    13441 tttcacttca tcaaaatcga caggtggggt aaaccacgcc cccaccaatt caccttcgtt
    13501 atatttcccg aggttcgcaa tataaacacg catctgttcc atcgtgcttc ccccttacgc
    13561 accgacaatt cggttaaaca aactgagtaa gacatccttc accccagaag cattaaagac
    13621 caatccgacc gcaatcaaag caatcaccaa aaagccaatc agtttggaaa actcccgctt
    13681 aaatcctaaa aacaaaccaa tgaccacaat tgccagtaag actaaggatt gggcattggt
    13741 taaaaaccat tgatatatgg ataggtaagt tcctgatatt ataaatatct caaacttttc
    13801 cccccattca cacggagcgt gcgactttca ccgcactccg cgttccatcg tgtttatatt
    13861 gaaaagtatt tatgtagtag acctatatca agtttaattc ttgattgcca gctaatactc
    13921 tatatttatt tagtcttatg agctggactt tatttagctt caatagagct atatattttt
    13981 gtattgtttc ttctgtttta gcgtgtatta gacggtgtac cgcctctttt accaaaataa
    14041 gatttttata atcgtcattt ccaccagctc cagttggtac aatatgatga caatgtattt
    14101 cattagcttg ttgaaattct tccagagtta tagcacattt tccatgttga gcagaaaata
    14161 gcgaaattcg attgtctgcg tattctattg tgcgattttc caaagataat ttcattaact
    14221 ggtacatcag taatttatca acacttagat tggtgtggat aaattttctt ccctctagag
    14281 tgtagcaatt cgctaaatac acctttgcca ttggttttct gtgttggata tatccaacag
    14341 ggaaaatagg ttcgccctcg ccagcaacat accgaagcat tttagattta ccatatcttg
    14401 ttgcctcctt tgtcgtaagt tgtctgcctg tttttctcaa tctgcccctt ttgtttgtac
    14461 ctaatctgtt agtgaagata gccattacag ctctattcag tttcatacaa tccagattta
    14521 cattcgttgc aatctgataa taattttgca tacctaatac cattgaatta taaagtcttg
    14581 tttcttctaa ttccgttttc ccttttgagg gagaagcaat tttctttgcc tgtgatacca
    14641 gcttgtcttt ttggtttttg aattgtttat cacttatatg tgatttaaca actaacttct
    14701 ttcttctcgg tatcatttta atcttgaaac caagaaagtc ggagtatctt tttctaacat
    14761 ttacaattct tgttttttct tgtgatactt ccaattttaa tcgttgttca atccattgag
    14821 tgattgctat tttggctttt tcagcacttt ccttgtagcg gcagaaaact ctaaaatcat
    14881 ctgcataacg aaccatatac atttccttta actttgtttt cttcatttct ttgtaagcgt
    14941 tgcttttaca tgggctaccg cttttattga tatgaaccac atagtttttt gtaacaggat
    15001 tttcctgcca ttggctttct acccaatggt ctaactcatt aagaacaatg tttgctagta
    15061 gtggggatat tattccccct tgcggagttc ctttctctgg atatgttatt gtaccgttag
    15121 tcattttgat tggagctttt aatatccttt ttaaaatgaa tatcaagtgc ttgtcccgta
    15181 ttcccatagc ccatatctgt tttatcagct tggaatggtt cacgttgtca aagaaaccct
    15241 taatatcaaa ttcaatcaca taatgtagct ggctgatttg taggcggtta taggttgctt
    15301 ttattgcatt ttcaactgac ctattaggtc taaaaccata gctgttttca ctaaatttag
    15361 cttcgcagat tggttctaat acttgtttta tacattgttg tataagcctg tcccacatac
    15421 aaggaatacc tagaggtctg gttttaccat ttggttttgg tatttctttt cgcctcactg
    15481 gcttaggtcg atacccatgt ggactgcctg caacaatgta tcttactttt ttgacaactg
    15541 tttctgctgg caatttgcca atatctttaa ttattgtacc gtctgttcct gctgttttac
    15601 tgcctgcatt tgctttgata tttcgatatg ccagtaatat attttctctc gataggataa
    15661 tatccataag atgagtgaaa atatctccat tcttactttt ggcatataaa tcatcaaagg
    15721 tgttttgcat ttcgtaatat tcagcgtgtc ttaaatcttc tacacacaat gtttcatttt
    15781 tatttttagg cataaggctt cactccttta cttgaaattg agtgagcttt tttatcttac
    15841 tcgtaatcct tattgaaaca acataatact tttctaatat atacacgact tgagggcatt
    15901 cctccattct cattacaaga atttcttagg ttcgccctct gcttttcagc attagttcaa
    15961 ctgcttattg ttcttcgtca gtttattctc ttgatgagaa cccaatacct ttcctcgttc
    16021 cgataatctt atctatacat atatatcctt aggtcactag ctataagcct gctggcttga
    16081 taccgcctgt aacgttatat ggcatttcat aacctatatt cttactttgc cacaccagct
    16141 acgatttctc gtacctttgc atttctacaa agttcaaatt tagacccgta cattcgctgt
    16201 ttcgtcagtt ccttatagaa cattctaacc atagatattt tatagacccc cgacctgttg
    16261 gatacgctac ccacctctgg atagttttcg atagaagata tatgttcatc aactattacg
    16321 gtatctttca gccgtcttta ccgagcttca aacattctga gtttcctcat acaatgcttg
    16381 tcggaggctc agggcaggcg tttcagagcg ttacttcatc attccacctg tcagattatc
    16441 agttctccta ataaccaaac tttttcgttc ttttattagt gccttagctt tt
//
LOCUS       DAZULO010000062        16382 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000062 DAZULO010000000
VERSION     DAZULO010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.063"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(152..550)
                     /locus_tag="ACG8OA_001924"
     CDS             complement(152..550)
                     /locus_tag="ACG8OA_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFS4976692.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="ACG8OA_001925"
     CDS             complement(547..1320)
                     /locus_tag="ACG8OA_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFS4976693.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="ACG8OA_001926"
     CDS             complement(1317..2000)
                     /locus_tag="ACG8OA_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFS4976694.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="ACG8OA_001927"
     CDS             complement(2167..3054)
                     /locus_tag="ACG8OA_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4976695.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="ACG8OA_001928"
     CDS             complement(3051..3920)
                     /locus_tag="ACG8OA_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4976696.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="ACG8OA_001929"
     CDS             complement(4057..5343)
                     /locus_tag="ACG8OA_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4976697.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="ACG8OA_001930"
     CDS             5616..6194
                     /locus_tag="ACG8OA_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4976698.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="ACG8OA_001931"
     CDS             6194..7123
                     /locus_tag="ACG8OA_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4976699.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="ACG8OA_001932"
     CDS             7120..8856
                     /locus_tag="ACG8OA_001932"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4976700.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ACG8OA_001933"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ACG8OA_001933"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFS4976701.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="ACG8OA_001934"
     CDS             complement(9752..10261)
                     /locus_tag="ACG8OA_001934"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFS4976702.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ACG8OA_001935"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ACG8OA_001935"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFS4976703.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="ACG8OA_001936"
     CDS             complement(11562..12653)
                     /locus_tag="ACG8OA_001936"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFS4976704.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ACG8OA_001937"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ACG8OA_001937"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFS4976705.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ACG8OA_001938"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ACG8OA_001938"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFS4976706.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ACG8OA_001939"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ACG8OA_001939"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFS4976707.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4728 a         3621 c         2912 g         5121 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tgcaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccga tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aa
//
LOCUS       DAZULO010000063        16233 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000063 DAZULO010000000
VERSION     DAZULO010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.064"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..270)
                     /locus_tag="ACG8OA_001940"
     CDS             complement(25..270)
                     /locus_tag="ACG8OA_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4976708.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(264..665)
                     /locus_tag="ACG8OA_001941"
     CDS             complement(264..665)
                     /locus_tag="ACG8OA_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFS4976709.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(837..2645)
                     /gene="uidA"
                     /locus_tag="ACG8OA_001942"
     CDS             complement(837..2645)
                     /gene="uidA"
                     /locus_tag="ACG8OA_001942"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFS4976710.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            complement(2673..4439)
                     /locus_tag="ACG8OA_001943"
     CDS             complement(2673..4439)
                     /locus_tag="ACG8OA_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFS4976711.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            complement(4452..5246)
                     /locus_tag="ACG8OA_001944"
     CDS             complement(4452..5246)
                     /locus_tag="ACG8OA_001944"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4976712.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            complement(5257..6633)
                     /locus_tag="ACG8OA_001945"
     CDS             complement(5257..6633)
                     /locus_tag="ACG8OA_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4976713.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            complement(6762..7409)
                     /locus_tag="ACG8OA_001946"
     CDS             complement(6762..7409)
                     /locus_tag="ACG8OA_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFS4976714.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(7493..8920)
                     /gene="uxaC"
                     /locus_tag="ACG8OA_001947"
     CDS             complement(7493..8920)
                     /gene="uxaC"
                     /locus_tag="ACG8OA_001947"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS4976715.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(8994..10073)
                     /gene="uxuA"
                     /locus_tag="ACG8OA_001948"
     CDS             complement(8994..10073)
                     /gene="uxuA"
                     /locus_tag="ACG8OA_001948"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS4976716.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(10076..11707)
                     /locus_tag="ACG8OA_001949"
     CDS             complement(10076..11707)
                     /locus_tag="ACG8OA_001949"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS4976717.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            12024..12695
                     /locus_tag="ACG8OA_001950"
     CDS             12024..12695
                     /locus_tag="ACG8OA_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4976718.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            complement(13531..14985)
                     /locus_tag="ACG8OA_001951"
     CDS             complement(13531..14985)
                     /locus_tag="ACG8OA_001951"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976719.1"
                     /translation="MFDESLHLFPEYPDTVGSQAIPEKKQAVCQILLKEEVQLKQELT
                     AVILMDRICKEVLAGSDGTLAYGVVPLFDEVVRLLGNTQQIGAICGEFLLLPKMLVTV
                     ELAELATGMNEHPYMSGYFYLTVDDKNRQALMQAVTRLLTEKKLNILVGTVSLLGEGW
                     DCPAINLLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVPLSEQPDLK
                     RMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSCERSRLKEQ
                     WSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKGNLLEYFQI
                     RRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLENAGYREGQ
                     LFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQSSLPKCQLV
                     YTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(14986..15285)
                     /locus_tag="ACG8OA_001952"
     CDS             complement(14986..15285)
                     /locus_tag="ACG8OA_001952"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976720.1"
                     /translation="MESQETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHN
                     YELIPEHWQLLGSSKQQGKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(15588..15959)
                     /locus_tag="ACG8OA_001953"
     CDS             complement(15588..15959)
                     /locus_tag="ACG8OA_001953"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS4976721.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
BASE COUNT         5104 a         3259 c         2242 g         5628 t
ORIGIN      
        1 ataagtggaa aggggacttt ttagttagtt aattttaaat tttaagatcg cttctatcaa
       61 tttaatttct agctgtctta gcatatactc atctacatag gtaataaatt gccccgcctc
      121 attaaatcca tttgtcaaac ataacttagc tatataacta tgataatgtt tgagtatcat
      181 cttgattgaa attggatctt cttctagagc agattggata atggatagag gaactaatgg
      241 atactgccgt tttttggggt tgtctaccat aagtcatacc ccatttttaa ttgttcaact
      301 gccttctttc gttgatacca gataccactt gtactcatat tgaggatttt accgatttca
      361 gtatcattaa aacctgcaaa ataataaagt aggataacct ttcttcttat ttccgataaa
      421 tgatcaatgg ctgccgataa cttttgatct gaaattaata tttccattcc aaataagaaa
      481 tagacatctt gttcatcaaa atgagtttgg gagtacttac cttcatttga attcgtcaat
      541 ggttcctcat tatctgttat gttaatgttt cgttttcgaa tagacttagt ttcgtttcgt
      601 aatacttttt tacagtaggc atcaaattga tgttgtatag atttctcatt gtaattttgt
      661 tgcatgataa atatgctcct tttttcccct ttccactaat acaacaatca gaaagcgcct
      721 ttttttcgaa aaaactagaa aaaaatatcc ttctttaaac agaaaatctt tttataaaaa
      781 aaaggagttg gcaattaagc agccactcct tttttatatt ccttataaaa ggtaaattat
      841 tttttataac tgaaatttgg tattgatgac catcgtttct ttagatagtg agcaatcatt
      901 ttaggctttc tatctctagt aaagattcct ttcttatttc cttgcactct taaaatccct
      961 tgacttgttt caaaatctgc aaaattccac acttgttcac caacaaaatt gtcacattta
     1021 tctattactt cgtgatttgc ttcgtaataa cccacttgat actcttctgt aaacattgtt
     1081 ggtactgtat catgaagtcc agctactgta tctgctccat attctgtaaa cattattggc
     1141 gtatcgggac atctttccat ccatccatct agctcttttt tcaacagata ttttgcatgt
     1201 tttaggtctc cacctgaaac ataccaacca taatagcgat ttaaacataa aacatcgaca
     1261 atatctccta ctttacaaat atctggtgtt gactttaagt aagtaacgat tgttacaggt
     1321 ctcttctgag ggtcaagttc tttagtcaat tcaactaaag gttcaaaata ttctttagcg
     1381 ccttcagaat ccgaatctgg ttcatttgca atagaccaca ttacgacact cggatggttc
     1441 ttgtcgcgtt ctatcaattc cttaatcgcc attttgtgag cattttctgt ccgtatttct
     1501 ttccaagtat catgtttttc tacgttgtca tctaataaag tagccatgaa attcaaatgt
     1561 aatcctactg caggtacttc atcaataact acaatacctt ctcgatctgc taagcgcata
     1621 atttcttctg aataaggata atgtgaagtc ctaaatgaat tagcaccgat ccatttcatt
     1681 aaattaaaat ctttgatatt tactgcttca ttcaaaccac gaccattaat atacgtgtcc
     1741 tcatgcttcc caaatccctt aaagtaaaaa ggtttattat taatcaaaaa cttcccattc
     1801 gaaacctgaa tactacgaat gccaaattct tctgtgtacg tatcaatcac tttattttcg
     1861 ttaattaaac taactttcat actatataag taagcattta gaggctccca aagtgttggg
     1921 ttcttaagtt gaatcactcc ttctttcccc ttagcttctg ctataagttg agcttcttcg
     1981 tcatagattg ctattagaat ttctccggtc cattctgtat cgatattaaa ttctacattt
     2041 gcatctgaat caattatttt tgtattaata gtgacatcct ttatatagtc tttcggagtt
     2101 gtatacaatt taacgggtct attaatgcct gcataattga aaaagtcaaa attaaccaaa
     2161 ttttcttttt ttatgtctcc atcttcctgt atcgtttctt tcaaaatccc tacaggtaaa
     2221 gtgctatcgt caataacatt gtttacagcc actgtaattc tgttacttcc atcttttata
     2281 aactgattaa tttctgattc aaatggcgta aaccctcctt tgtgttgatt aatcaatttt
     2341 ccattcaaaa atacttttgc ttcatgtgtt gcagatccaa aacgaagaac taatctttct
     2401 tctaataatt ttttaggaag aatcacttct ttttcatacc acacccatcc aacatgattt
     2461 ctaatgttct ctgtttcaaa taaatcatta taagaagaag gaactgaaat tggataactc
     2521 tctttttcaa ccattggttc taaagcaagt tcttcttgta agtcatcatt tgtatctaac
     2581 ttaaatctcc aaagaccgct taaatcaatt acttgcctcg tctctgtcat aatgggatat
     2641 aacatgtaaa tcactcctat ataatttaaa tattaaataa atgtatcctt taacccacaa
     2701 taagcatcta ttggacttat tcccttaaat tcagaaaggc ccattagttt gtccactaat
     2761 atttctaaag tttcttgttt tccatcataa gtattgatat aagtttttac ttgaggcaca
     2821 tcagctaaat gaaacggaca ctgtacagat acaaaaattg ttggaatttc atgaatataa
     2881 aatggaatat ccggagtacc tttacttgct ggccaaacaa ttctttgaac tgtacctggg
     2941 ttaacattag ctatattaat aatcaagtca tatttttcag ttaaatcttt gattggtctc
     3001 ttagcagcat atattccagc tatattttta attttttctt cttgcgataa tcctttcaat
     3061 tcttcttcag gtgaaatgta tttttcaact tcaaatcctt ttgctgacaa aatctgtttc
     3121 aataaatcaa tggcctgagg tctatttcca gcaactactt caccaaaccc accttttacc
     3181 ccatgtacat ctaccagtaa aatcctttta tatttttctg gtacaatagg ccaaatatct
     3241 tgtttatctt taactagtgt aattgcttga tcagctacct ctttaaaaat tgatttattt
     3301 tcaattaaac caattttttc catagcttct tgtttcggta acaaaatttt attcttttct
     3361 tttttatgca aacctagttt agcctttaag cctaaaattc ttcttaatgc atcacgtaaa
     3421 cgctcttctg aaataattcc tttgtaataa ccttccatca tataattaaa gtcttcatct
     3481 ggatcattaa aaaataggaa tagatcacat ccagccgcaa tagaagtggg caataattct
     3541 gaccgtttca ttgccccagt cattcctaac atatgactag catcagtaac tactaatcca
     3601 ttaaacccga gttttcctct aagtaagtca gtgattaatt cttttgataa agttgcagga
     3661 agtaaatctt catctttcag atcaagatta aaatgttttg aataagctgg catatggata
     3721 tgacctgcca taattgaagg taatccagct tctattaagc ctgagtatac ttttccaaaa
     3781 gaattatccc attcttctgt acttaaacta ttaatactaa aagatagatg ttgatctcgt
     3841 tcgtcaatac cgtcaccagg gaaatgttta gctgcaggca aaattccact ctcttgaata
     3901 cctttcatat aagctaaact aagttctaaa actttatcag ggctttgtcc aaaagatcga
     3961 ctagaaatga taggatttct ccaatttcta ttaatatcta caataggagc aaaactccag
     4021 ttacatccaa tagccgatgc ttcaattcca gaaactctcc ctaattcata agcatatttc
     4081 gcatcatttg ttgcaccaat ttttacttct acaccaactt cggtaccatc tgtacaagct
     4141 ccgttaccac ctgcttctgt attagcagca attaataaag gaattttgct ttctgtttgt
     4201 aggatatagt tttgatcata tacctcatca gcagttcctg gattatatcg tacagctcca
     4261 atatggtact gattgaccat actacttaag taatttttat cacgactaga tcccatatta
     4321 acaaataatt gacctatttt ttcttctaaa gtcatagcat caatcgtctc ttcaatccaa
     4381 gaaatagcat cttcatctaa atgatatggt ttttttcgta agtctacttt tatagtcatt
     4441 ttctttctcc tctacttatt agtaagattt ttccaaaaag tatctatata tttttgttca
     4501 atatctatat actctcccga taaacattta aataaaatat cagttcttaa gttttcccat
     4561 gattcttttt ctttattaac caaaatagga taaaagaaaa ctttattctt atttgcagct
     4621 tctagatcac caggagaatc accaatcatt aatatcttat ccaaatcaca accattttca
     4681 attacacttc gaataatgtc ttcttttttt cctttatctt gacaacacag ttcatcaact
     4741 aaggataata attcatgtct ttcccattct tcttgaatag cttccttatt agctgagctt
     4801 acaataatta tcttcccata ttttttcaga gcttttatag tatctctagc acttaaaaag
     4861 ggtttatcta atcctttagc ttgactaatc tgtcgattga cttcttccga ccattttaaa
     4921 gcttttaata aatcctctga ctgagagtat tctatttccc tcttcaaaga tggattagac
     4981 aattcttttg ttacagatac ccagtgcttt aatcgattaa tcgatggtac atcatttttt
     5041 tctaatgttt taactaaacc taaaaatctg tttattccac gagtctctga atataaatta
     5101 atattattcc aatcctgtag gaatttttcc ctatgatgaa catcaaaaat ctctgcagca
     5161 attggcccga aaaagagatt gtgtttataa gtcattgtat ccatgacaca accatctgaa
     5221 tcaatacaaa atatccaact gttcatgttt tttctcctag ttatgttttt gatttagcaa
     5281 attataattt tctaatactt tttctttaga taatggatat acgaaaaata aaatcaatcc
     5341 tacaacaaag aaacaaactg ctggaatcaa cgttgaagag ttatacagct tttgtaaaac
     5401 ttctccactt tgtacttttg ctaacgaatc ataccctatt atagaaagag tccaacctcc
     5461 gacaccgcca gctaaagctt gtccaatttt tcgggcaaaa gaatatactg cataaatagt
     5521 tccatcttct ctttggccag atttcacttc ttgatcatcg attacatcag taatattcgc
     5581 ccaaatgact gtattaaaaa tacctaaccc catatatcca acagaagtaa gaactaagaa
     5641 tacatacata ttactaggct ttatcaaaaa taaaagtaaa tatacaatag cagagaagaa
     5701 catccctact gacgccaatt cttttttacc gtatttttta ctgaaaatta aacttacagg
     5761 tgaaacaagt accaacatca aaataggatt gataaagttc attaaaacta ttccctgtgc
     5821 attattataa taatctggaa atacataatt gttcatactc attgttaaaa gctgagcaag
     5881 caacaaaaaa atagctgcag ctattataga aagcaacgct ctatttttag ctaatccttt
     5941 taaagatttc aacaatgaaa cttttaactg cgattcatca cttttaaccc gttctacagt
     6001 taatttataa catattacgt aacaaataac tgcacacact gaaaatactc ctgccacaat
     6061 tgtaaaagtt gatccaccac gtacaatttg agctccagaa ctatctcttg tataaataaa
     6121 aattggtgtt aacacgccaa tgaacatacc tgctaaggta gctcctatac tacgaaatgt
     6181 tgataacgct gtcctttctt ctgcaacaga agtgatagct gatgccattg aaccgtaagg
     6241 aatattaata gatgtataaa aaatacttcc ccatagtata tatgttacat acatatatat
     6301 aactttaata ctcatagaag ctgatgccat tgatgtttga tacattaaaa aacttgctaa
     6361 agcgactgga ccactcatcc gtaaaatcca cggtttaaat ttgccagttt tagatggttt
     6421 tgatccatcc acaatacgcc ccattgttat gtctgtaact gcatcaataa atcttgctac
     6481 taaaaataat gttcctacgg tagctccgct tatccccaat acttttgtat aaaaaatcat
     6541 taaaaaagaa ctagcaaaaa tgaaagtgaa atcattccca aaatctccaa acatatatcc
     6601 tattttgtct cttagaccaa attttctttc catataattt gcgctccttc ctattgttaa
     6661 gcgttttcat tctgttgaac tgccaactta cgtactatac tctgaagtat aaagagttag
     6721 tatttatttt ttacatttta tagtattatt tattttttct cctaataatt ctttatctca
     6781 taaaacttat ctatatattt actagctatt cgagttactt cttcaaaatc acctttttca
     6841 acaggagcca acaaattccc acctacacca actgcaacaa cgcctgcttc aaaccactct
     6901 tctaagttat ctagacttac tccgccagta ggcattatat ttacatatgg taatggagct
     6961 tgatacgttt ttacaacaga tggaccaaaa gcagaacctg gaaatagttt aatgatttct
     7021 actccagcgg taagtgcctc ctttatctca gtaattgtca tacaacctgg taaataagga
     7081 atttgatata aattacaaat ctctgctgtt tgtcgatcaa aagatggact tacgatatat
     7141 tctgctccag ccataattgc aatacgagca gtaaacgcat ctaaaactgt accggctcca
     7201 attattacat ctttttgatc agcatatttt ttagcaattt ctttgattat ttcttcagca
     7261 ttcggaactg taaaagttag ttcaatccct ttaattccac ccgcagtaat tgcatttatt
     7321 gctttaatgg cctcatcttt attttttcca cgtacaaccg caatgactcc agccgcttct
     7381 aacttactta aaatttctac ttttttcata tctatctcct tcttttatgg atacatactc
     7441 tagctagttg tactaactta caataaataa atttaagctt ttattttttg tattattttc
     7501 tactttcgat ttcaaaaaaa ttaaaataat tatacgcatt acgataacaa atattctcca
     7561 ctatctgact aagatacatt tcatcatctg gaattcgacc tgctttaacc cactcgctta
     7621 gtaattgaca taatactcga cggaaatact catggcgagg atagcttaga aaacttcgag
     7681 aatcagtcaa catacctgta aaattcgaca acaaactttg ttgcgccatc gtagtcaact
     7741 gttgacgcat tccttcagct gtatcattga accaccaagc acatcccaat tggatttttt
     7801 gaatcatacc cccttggaag ctttgcattg ctgttgctag tggaagccaa tcggttggat
     7861 ttaatgaata taaaattgtt tttggtaagc acccactctt ttcagcagtg tcgaacaatg
     7921 ctacaacatt ttgtgtcaaa ctagcttgat cccccatcga atcaccaccg acatctactc
     7981 caatttgttc ctttaatata cttgaattat ttcttaaagc atttaaatgc atttgcatcg
     8041 tccaaccata tcttgtatac tcagacatca aatgtaattg aatacctgta acaaattgtg
     8101 caatttctga taaatggaaa tccgtttttc ctgctaaagc ttttattaac agattgtcga
     8161 gtattttttc atctgctatt tcataaaaat aagtattcaa accgtgatct gctaatcgac
     8221 agccaatttg atgaaaataa tccactcgtt ggctcaatgc ctgcttcaac ccattaaagt
     8281 ttttgattgg tatacccgct gcttgactaa gttttaaaat atagttcgaa aatcctgatg
     8341 atttgatact ccagacctta tctggacgga aagtaggtaa taccttgaaa gaattatcct
     8401 gtttagaaag ctcagtatga tatcgcaagt catctgtagg atcatccgtt gtacaaatgg
     8461 ctttcacatc catcatcttc agcaattctt ttgggcgata ggaatccgtt tggatttttt
     8521 cgttggcacg tcgccaaatt tctggtgcat tcttctcaga aattgtcaaa tcaatatcaa
     8581 agtaacgttg taattccaaa tgagaccatt caaaaatagg attacctggc gccttctcca
     8641 ctgtttttac aaaagctaaa aatttttcat aatcattacc tgcaccactg atcaactctt
     8701 cttctatccc attagctcgc atcaatcgcc atttataatg atctccagct cctttatcat
     8761 ttaaccatac ctgagtcaaa ttcttgtatt gcttattttc tagcaaacat gctgggtcta
     8821 aatgacaatg ataatcgatg attggttggt gttctgcaaa tttatgaaaa agtcttttag
     8881 ctatttcatt ctctaataaa aagttcttat ctaaaaacat atttaagcac tcctttcctt
     8941 cccttagttt ttagaagaga aaatttttct tctaaataga ctccattata taattattta
     9001 ctcgccccct tttcaagcgc ttcccaaata ccatttaaat aaactgcacc tagagctcgg
     9061 tcatataacc cataaccagg tctaccggtt tctccccaaa tcatccgacc atgatctgga
     9121 cgaatatagc cattaaatcc attatcgtac attgttttta atatcttata catatctaga
     9181 gaaccatatt ctgatttatg cggcgcttca taaaaatcga gtccattatc acttaaatat
     9241 ttgatattac gaacatgtgc aaaaggaatc cgatcccgtt gacaaaattc agatagaatt
     9301 tcatagacat cattatctgg tcgttctgca atacttccag tacataacgt aataccatta
     9361 gaaggagaat ccactacatt acacaaccat tctaaatcgt cacgattttt aacaatacga
     9421 ggtaatccga aaatagaata tggtggatca tctggatgaa ctgccatttt aatcccaact
     9481 tcttcacatg tcgggataat tgctttgaga aaatatgcaa agttctctcg caatttcgct
     9541 tcatcaactt ctttataggc ttctaataat tgcttgattt gtgataaccg atgaggttcc
     9601 caacccggca attcaaaatt attagtagaa ttttctactt ttctaataat ttcctctgga
     9661 tcagtaggaa tattttttga aatataggct aacgtagaag acccatcatc taaatgataa
     9721 tccaaatctg attttaccca atcaaagact ggcataaaat tataacagat aacctcaatc
     9781 ccaaattctg ccaaattgcg aatcgtctgt ttataattct cgatgtattg atctcgtgtt
     9841 ggcaaaccaa ttttgatatc atcatgaaca ttaacactct caataacttt caaggtaagg
     9901 ccattggctt cgacttcatc ttttaaagct ttaattcgtt ctctcggcca gacctctccg
     9961 gctggtatat ccattaatgt tccaacaatt cctttcattg ctgggatctg acgaatttct
    10021 tgtagtgaga ttttttcttc atttgcccca taccatctaa aagtcatttc catagttact
    10081 ttcctccaac ttctgttata tatttattga ttgttttacg aactgcttga ggacctacta
    10141 gcatttcgca aaagattttt tcaattttct ttcctatctc aacttgatat aaatctaaac
    10201 caaatatttc cttattttct aagatttcct gtaccgcaca atgaatcttt gcaggatctt
    10261 gttcacctaa tttcaatact gagaattttt cctgcaactc taccaataaa ggatcaggac
    10321 ttggcacaaa attttttcct tgatcatcga ctgccaataa ataacgtaac caagcagcaa
    10381 tagcgatagg aataaatgtt agattttctg catttccgtc tttttcgaca tactttttaa
    10441 ttgtttcacc atagcgaatc gctacttttt gagaagtatc tgttgcaatt cgttgcggtg
    10501 tatctggaat atagggatta ggcaaacgtt tattgattac ttcatcaata aattgttttg
    10561 gatcaataac gcctggactt tcaactactg gtaagccttc tacataacct atccctttaa
    10621 ttaagccaac caaatcatca tcttgcattt ctgcctgaat tgatttatac cccaacaaac
    10681 aacctgttat tgctaatgcc gtatggagcg gatttaaaca tgtcgtaacc ttcatgatat
    10741 ctgctttatc aactgtttct ctatctgtca taatcacacc agccgcttcc agtttaggtc
    10801 gaccatttgg aaaactatct tcaatgacta gataatggac tttttcagta ttggtaaaag
    10861 cagcgatatt ggtatttttc gcagtatgaa ttatatctaa gtcttcgaaa cccgccttca
    10921 ataaatccat tttgacactt tccgaaggat ttggcgtaat tctatcaatc atactccaag
    10981 gaaaagtcac tttttcttta tcctttaaat aagcaataaa tttttcagaa acgtatcctt
    11041 ttttttgcca agcttctgca atcataaaaa tgctatcttg aaaacgttga ccattctgtg
    11101 agaaattatc agtagaaacc atcgcaatcg gtaactctcc agcattaaac ctgctataca
    11161 acaaggatgt cacaatactc atcgtatgtt gaggattggt aggtccagat tttatatctg
    11221 cttccactac cgaaaaaaat tgtcctttag aatcttttaa actgtatcct ttttcagtaa
    11281 tagtaaacgt tgtaaactgt aatgtaggat cttcaaaata tttaaccact ctttgatagt
    11341 ccgttatatt ctttggattg caatataaac tatcagctgt tactgctaaa agtctttttt
    11401 tgagttcacc atcttcaccc ataacaatct ccaaaatatc attgttgtag tcatgataag
    11461 ctttttctac tacttcttca tcaaatgtct cacacacaac tactccggat tctaattcat
    11521 tttgatctgc taagcattga gcaatttctg catgaaatcc tcgataaaga tttcctccac
    11581 caaaatgaag ccacactgga tgtttctgac cttcttgttt gaccgcttcg acattaaaat
    11641 acggtagttg aacttgcatt ttgttcaatc cttcgataga atcttgccag tctttaatca
    11701 aaaccatatt tacccctcca tactgatatg ttagtttccg ttacaattgt agtataaaat
    11761 cgaatgtaat cgctgtcaat aagaaaaaac taaaaatatt cttttttcta ccatgtcaga
    11821 attattgtgt ctttttttag taaaagcaca ccaatatata ttttttagaa ctaacttaaa
    11881 ttaaagctta atttctcttt gatcttattt gagtattttt tgttttttta atagctagta
    11941 tgttactttg aaatttgata ttatataacg agcatagaac taaaatatca aattcaaaaa
    12001 tgaaattttg agagggatta aatatgacaa ttaaaactag tctccaaaat caagcttatc
    12061 aatctattcg tcagcaaatt atctacgctg acttagaacc tggaaaaaaa gtatcagaaa
    12121 aaaaattgga agaaacgctt tctatcggtc gtactccaat acgtgaagca ttgattcaac
    12181 tccgtcatca aggacttgtt gatacaattc ctcaaagtgg aaattatatc tcattaatcg
    12241 acttagattt agcaaaaaat gctcgttttg tcagagaaca tttagaacga caaattatgc
    12301 tagagtgttg tgccaagatg aataatcaaa agaaacaagt tttagaaact ataattgcag
    12361 aacaagaaaa agctgttagt caaaagaatg ctcgtagttt ttttcataat gataatttat
    12421 ttcataaagc ttgttttgaa attgcagaac gggaagaagt atggcaatgg ttggaagata
    12481 ataatacgca cttggaacga tttcgttggt tacgagtaaa aacggaagga ttaaaatggg
    12541 aaaaaattat ggaacaacat catcaactat ttaaagcctt gaccatgcaa gaccctgaag
    12601 aagctaactt cttaacatcc ttgcatcttc atttaatgct tgatgaacaa aatgttgtat
    12661 tgactactca cccaaaatat tttaaaaata tataactgct aaatttcttt tttttaattt
    12721 atttacattt ctgattacta cagaaaacta actacttttt ttatgtaaag ttctgtcaaa
    12781 tatagtcact ttatattact ttctcttgag taaaaaaaca tgtgaggtcg taaacaaaga
    12841 ttgaactatc tacattcata ctctataatc tgtttatttg cagactgata atagcaaaag
    12901 aaaacttata atcaaataat atcaacattt gatcacataa tttgagattg aatgctgatt
    12961 tacctatgtt ttacgtaatg gtctttagtt atttttgctc ataaaatgca taaaacacgc
    13021 gtttttatgt gtgcttgaaa tttcttggct ctataatttc taagactgat aaaaatattc
    13081 tatcggttct ttttgcaaat aaaaaaacat atagaagctc cattagattg aatcaccaca
    13141 acacaatcaa aggagcattc cgtatgcttg attttcattc tataaaacta cttttaggaa
    13201 tcaaagaaaa tcacaacggt tcaaactaaa agcaagaata tccgaagtac cattgtttca
    13261 tcgatcatga gaaaagcatt cacctctgtc tgcagatgtt cttatatcgc tctgtcagtc
    13321 ctattaataa agtactgttt tgttttcaaa aaaactatta cgagtacttt ttaagagaat
    13381 gccttgcaaa tattcatcac acaccgttat ataagtcaaa tagtttcttt cagtcgtcaa
    13441 gaaaagattc tcctgtcctt ggcattatca gttaagtagc aacttaattc tcggacttgt
    13501 tctactctag caaaatttat tttcatcaac ttacgtccaa atgttttttt gaataattac
    13561 ttcatcgcgg gtctggaaga cttgcctcat tcgataagtc actagctttt ggcgtccttc
    13621 aggatttcta gtatacacca actgacattt gggcagcgac gattgattta attcgtttag
    13681 aaaaaggtta gcgatttgct tatttcggcc aaagcgttct ggtaccgtca gaactatctt
    13741 tttcccttcg attaaatacc gcggattctc cagcggtgct aaaagctccc tcatagcttc
    13801 agtaaaaagc tgtccttctc gatagccagc attctcaaga tagcatctaa attcctgctg
    13861 atcagccgct acgattactt ttctatcact aacttcacca aggcgctgca acgtcgtttg
    13921 aacgcatatg gccaagcttt ttttgcgggc acattccttt tcccatagct tttgtcgcct
    13981 acgaatttgg aaatattcca gcaagttacc tttaaatata gctgaccaga agcttagagg
    14041 ctctatattt tttactacta tcggcaatga agtttcttgt ttttgcttta cctgatcgac
    14101 tttcctaacg acgattggca tagataggtg ccagccatca gctaaagcag tactccattg
    14161 ttctttcaac cgacttcgtt cacaagaaaa atagaacatc gactgagtat aagtttccat
    14221 cgtggtggtc gtatattcca gtggtaaagc aaagcgatcg ccaccactct caatttttgg
    14281 tgaagattca aaggtcagac ctgaaatatt gcccaaacgc cgtagcattc ttttcaaatc
    14341 aggctgttca gacagaggaa ctcccggtaa tccaatcgct aaatgccaaa tgttcgttac
    14401 tttattttgt gcacctaggc gtaagccccg ccctcgtagt tgttgtgttt gcacaaaagt
    14461 ggaagcttga ttagccaaga tcaacagatt aattgcagga caatcccagc cttctcctag
    14521 caaactaacg gttcctacca aaatattcaa cttcttttcg gttaataggc gagtaactgc
    14581 ttgcatcaat gcttggcggt ttttgtcatc caccgtcaaa taaaaataac ccgacatata
    14641 aggatgttca ttcatacccg tcgctagttc tgccagctca acagttacta gcattttcgg
    14701 taacagtaag aactctccac agatggcacc gatttgctga gtgttaccta atagacgaac
    14761 aacttcatca aacaaaggaa ccacgccata agctaaggta ccatcactgc ctgctaatac
    14821 ctccttgcaa atacgatcca tcaaaatgac cgctgtcagt tcttgcttca gctgaacttc
    14881 ttcttttaac aaaatttgac aaactgcttg ctttttctca ggaatcgcct gactaccaac
    14941 agtgtctgga tactctggaa acaaatgcag cgactcatca aacaatcatc cagctttttt
    15001 caattgataa aaaataggca attccggttg atcttcaaac atctgctgat aaatagtgat
    15061 cagctctttt ttacctaaag atggaactga agctttttta ccttgctgct tagaactgcc
    15121 taatagctgc caatgttcgg gtatcaattc atagttatga tgattcaagt aaattaatgc
    15181 actagtgaac agctcaaaat tttcataaat ttccttcgaa tgctcaagcg gatgctgaac
    15241 aaattctaaa gaaagcaaat aatccaccgt ctcttggctt tccattaacg cttccaccag
    15301 ctcattttgt ttctctaaaa aggtaaaata atgctgagta gtttcctgat tagccggaat
    15361 caaatataga aaatcctgat gaggacatag aacctcctcc tttaccaact cgggaatgct
    15421 aatttcttcg tcaatcaggc cattttaact caatatattg cttccactcc gtctgagagc
    15481 tatcaatcgg aggcgtggct gtcaaggaaa tcgtcaacag attcggttgt tttttcttca
    15541 agtcaatcaa caattcaccc cagctttttt ttaaatgatg agcttcatca aaaaactaac
    15601 agctccagct gatcaccata agttggctcc tccttcagtc ggttgtaaat ggtctgatac
    15661 gtttccactg taatatgact aggatgttca atcgccccag aaagatagca actatctgct
    15721 ggatttacta agaaatattc ctcgattgtt gttaaccact gtttcttcaa tattagggaa
    15781 ggaaccaaaa ttaaggtttt acaatccaaa cgtcggatca tctcaattcc taaaaaggtc
    15841 ttgccagccc ctggtggagc cactagatgg actgaattgt tatttaaata agtagcaaag
    15901 ttagtcaata tacgcttctg atagctacgc cactgaattt tttcatcaga tagccacatc
    15961 ccaggtcccc ctctcacgta ttttctctat cataatctaa atgaatacag caaaaaaaag
    16021 atttcttcaa gaatatgtgt ttttatagta atattttact tactctaaac tcaaatgaaa
    16081 aggtaaaaaa actggtagta caatctttta aattctcatt gttttgtacg ttctttacta
    16141 tcaaaaaaac aaaaaaaacg cgtttctacg cgtgaccaaa atttttagct ctataatttc
    16201 taagactgat agaaaatcat tctgtttttg aag
//
LOCUS       DAZULO010000064        16195 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000064 DAZULO010000000
VERSION     DAZULO010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.065"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..459)
                     /locus_tag="ACG8OA_001954"
     CDS             complement(<1..459)
                     /locus_tag="ACG8OA_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFS4976722.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLA"
     gene            complement(589..1299)
                     /gene="budA"
                     /locus_tag="ACG8OA_001955"
     CDS             complement(589..1299)
                     /gene="budA"
                     /locus_tag="ACG8OA_001955"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFS4976723.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1312..2976)
                     /gene="alsS"
                     /locus_tag="ACG8OA_001956"
     CDS             complement(1312..2976)
                     /gene="alsS"
                     /locus_tag="ACG8OA_001956"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFS4976724.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3184..3846)
                     /locus_tag="ACG8OA_001957"
     CDS             complement(3184..3846)
                     /locus_tag="ACG8OA_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS4976725.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3856..4671)
                     /locus_tag="ACG8OA_001958"
     CDS             complement(3856..4671)
                     /locus_tag="ACG8OA_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4976726.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4932..5375)
                     /locus_tag="ACG8OA_001959"
     CDS             complement(4932..5375)
                     /locus_tag="ACG8OA_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFS4976727.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5509..5847)
                     /locus_tag="ACG8OA_001960"
                     /pseudo
     CDS             complement(5509..5847)
                     /locus_tag="ACG8OA_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5835..6212)
                     /locus_tag="ACG8OA_001961"
     CDS             complement(5835..6212)
                     /locus_tag="ACG8OA_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4976728.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6436..7629
                     /locus_tag="ACG8OA_001962"
     CDS             6436..7629
                     /locus_tag="ACG8OA_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4976729.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7795..8217)
                     /locus_tag="ACG8OA_001963"
     CDS             complement(7795..8217)
                     /locus_tag="ACG8OA_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4976730.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            8587..9294
                     /locus_tag="ACG8OA_001964"
     CDS             8587..9294
                     /locus_tag="ACG8OA_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976731.1"
                     /translation="MKKLVALFLVAGSLLSGCGATTSTTQSTSSAQASSSSTTISEST
                     ADSSKEQIEKKKEEEKQKAEKAALEKKVTEAESALSQAEANPTKENYATASAAIQALP
                     ENKQELTSRLAAIDSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAI
                     AQQQAEQARQQAAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATL
                     SEAQARGLTPCSKCYGN"
     gene            complement(9456..10628)
                     /locus_tag="ACG8OA_001965"
     CDS             complement(9456..10628)
                     /locus_tag="ACG8OA_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4976732.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10834..11649)
                     /locus_tag="ACG8OA_001966"
     CDS             complement(10834..11649)
                     /locus_tag="ACG8OA_001966"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4976733.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(11799..12245)
                     /locus_tag="ACG8OA_001967"
     CDS             complement(11799..12245)
                     /locus_tag="ACG8OA_001967"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFS4976734.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(12242..12757)
                     /locus_tag="ACG8OA_001968"
     CDS             complement(12242..12757)
                     /locus_tag="ACG8OA_001968"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFS4976735.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(12830..13744)
                     /locus_tag="ACG8OA_001969"
                     /pseudo
     CDS             complement(12830..13744)
                     /locus_tag="ACG8OA_001969"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(13741..16068)
                     /locus_tag="ACG8OA_001970"
     CDS             complement(13741..16068)
                     /locus_tag="ACG8OA_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFS4976736.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         4616 a         3234 c         2881 g         5464 t
ORIGIN      
        1 tgctaataaa agatctacat atttttctag aaatgcttga tcgatttcat catacccttt
       61 cgttactccg caatcgatat ctaaaacacc gattaaatta ccttctttta ccattggcac
      121 tacgatttct gattgtgcgg ctgaatcgca ggaaatgtaa tttgcatggg ttttgacatc
      181 atcaacgatc aatgtttctt gtttttcagc tgattctcca caaactcctt ttcccatctt
      241 gatacgtgtg caagaaactc gcccttggaa cggtcctaaa atcaattcat taccatcgaa
      301 taaataatat cctgcaaaga cagtctcagg caaagtctca ttcaacaatg cagatgaatt
      361 tgctaaattt gctaaccagt tatcttctgc ttctaataat ccttgttgct gtaataataa
      421 aatttcgtat gcatcttttt tctgtttttc tgtccacatg ataaaaaccg ctcctttttg
      481 atctatggag aagattatag ctcagttttt ttgtaaacgc taggtttcct cctcaaaaaa
      541 acgagatacg tcaggttaat caccttgaac gcatctcgct gtctttttct attctgcttg
      601 ttggatttct gctgctaaat cagcataatc aatcgtttcg tgaacaaaag tcggatcatc
      661 aactggaaaa tgctgtacaa gggacgagat gcggctaatc tcaacttttc cttcagccac
      721 ttcaaaatcc attacatgtc cgccaaatgt atgtgtatca tcgataaaat gcaggtgaaa
      781 tcccgctgca gcaactcctt ggaataattc aggcgtaaag aacccgacta atgttccttg
      841 gatattgctt tgttggaatt ctggttgaga ctctgaaatt tttgctagtc ttctgtaagg
      901 tttttcttgc ttcggcatga tacggatgtg catatttttg aataatccag ttatcttcac
      961 tgccaaaaat aggttttccc cggcttctct tactaatatg tcttttttca attgttctcc
     1021 atctgcagtt tttgagacag aaaaactagt atctggcgta aaatctgtaa ttgctgcata
     1081 gggggtaagt tcacttccat ctaattgggc aactgagcca tctgatcttc cttgataagc
     1141 tacgccatct aaaaagatga cttcaccatc aagcccatgc aatgttccca atcccagagt
     1201 tcctttttct aatatatgag caatcgtttc tgttccatcc atcaagccgg ccatcaaagc
     1261 tcctaatgtc ccgtgctggt ataagatttt ttcagtcatg tcgacacttc cttaatagaa
     1321 ttgatctggt aagattgttt ctcccaattt ctcattatca cggtaatcaa tcggaatatc
     1381 aataattacg ggtccttctg tcgcaaatcc ttcttctaaa gctttttcta attcctctac
     1441 acttgtagct cggattcctt tagcgccaaa agcttctgca tatttcacaa aatcaactgg
     1501 accaaagtct acacctgacg cccgctgata tttcattttt tcttggaact ctaccatatt
     1561 gtagtgtcca tcattccaaa tcaaatgaac gatattcaat tttttacgta ccgccgtttc
     1621 taaatcctgg gcagagaata aaaatccccc atcgccggaa actgatacga tttgtgtatt
     1681 tggacgcacc aatgcagcag aaatcgccca tggcaatgca acaccaagcg tttgcatacc
     1741 attactgaat aacaaatgac gaggttcata acttctaaag tggcgtgcca tccaaatata
     1801 atgactacct acatctacgg taacagtcat atcatctgtt acttttgact gaagcgtgtt
     1861 aataacttct aaaggatgca agatacctgc ttctcctttt gattgaacaa tgtcccgctc
     1921 cgttaatttt gcttgaagag atgctaaata ttcttttgca tcttctgaca cttgttgagg
     1981 ctctaatgaa cctgtcaata aatcaagagt ggctgagata tcaccgatca attctctttc
     2041 aggctgcata aaaggatcga tttcagctgg cgcttcatcg atcacaatga tccgcgcatc
     2101 tttttccgca ttccagtttc ttgcttcata ttcgattgga tcatagccaa ttgcaataac
     2161 tagatcactt cgtttcaaaa gcatatctcc tggttggttt ctaaataaac ccacacgacc
     2221 aaagaaatgc gcttctaatt cacgtgagat cactccagct gcttggaatg tctctacaac
     2281 aggcaatgct gtcttttcaa ctaattgcct gatggctaac gtttcttttt cacttgatcc
     2341 tcgcatacct actaatagta ccggtagttt tgcttctcta atgcgttcag ctaggtaacg
     2401 gatatcatct gcagaagcag aacctaattt aggatcgctc aatggcttga tgacattacc
     2461 ttggacaggt gcatccacta catcttgcgg gatactgata aagctcgcgc ctttttttga
     2521 actcttagcc atacgataag cattggcaat cacttctgaa attgtttctg gatcttggat
     2581 ttctgcactg tattttgtga ttggctggaa cagtgctgca ttatccatgc tttgatgggt
     2641 caacttcaat agatcgcttc gttttacttg tccggcaatt gctaaaactg ggtcaccttc
     2701 tgctgtcgct gttaccaaac ctgtagctaa gttactagca ccaggaccac ttgttgcgat
     2761 aactactcct ggctctcctg taatacgtcc gattgcttgt gccataaatg cagcattttg
     2821 ttcgtgacga gtcacgatta attcaggacc ttgatcctct aattcattaa atacaccatc
     2881 gatttttgca cctggtatac cgaatacata atcaacatga tgattaatca aactttctac
     2941 tacagatttc gaaccttgtt tcattttttc agccattgaa catcgtcctt tttatgcttt
     3001 atttttaatt attagttaga aataatcaca aataagtgat acagtttcta aacctgtttc
     3061 atacaaaagt atagcacgtt ttatttcatt tgtttagcca tatgtttcaa aaaatgaaaa
     3121 agaatgacag tttttacata aaaaaaactg tcattcttaa ttgaaagttt caatttataa
     3181 agtctacaga tccatagttc tcatcaattt acgattgttg aagtaaatca aaccaaatac
     3241 gatagaagga cctagtagaa tacaccattg gggctttacg aaaaagaaga gaaacaaact
     3301 tgtcaatagt aaaaaatcta gtaaactcac ataccatttt ttcaacgtat agtagaacgc
     3361 tatccgtaga acttgatttg gtttcgacgc tggatttctc acctgaaaat accagcagtt
     3421 ggtgctaaag gaaactgata aacaagcaag taaaacaaac aacggaatca accattttcc
     3481 ataagaaaaa cgtataaaaa agaaacaatc aaaaatcatt atcacactaa ctatccaagc
     3541 aaatagccaa taaaaaaatc cctttagcca aaattggcga aacatcttta tgaacaaacg
     3601 tactggtgaa aaactatttt cttttgctaa ttcttctgtc atacctatca tcacgatgca
     3661 acctggacca ataaaaagta aagaaaaaat aaaggaaaag acattccttc catctatcga
     3721 caaaaggaac gcagctgcta tatacaagat attgacacac caaaaacaaa ggctacttgc
     3781 caatgattga tacaaccaat aaaagaattc catataaaca tttttgtcaa aacgctgctc
     3841 tttcataatc acctcctatt ccttcattcc tgatgaagcg atcccttcta caaaatattt
     3901 ttgcatcgct aaaaatataa aagcaacagg aagaacagaa acaactgagg cagccatgat
     3961 taatgcgtac tctgcatcaa atagcccaat aaacattttc aaccctaact ggattgtttt
     4021 attttccgta gaagacaaat aaatgaatgg ccccatataa tcgttccatg tattaacgaa
     4081 tgtgatgatc gtcaaactgg caatcgccgg ttttgtaaga ggtaaaatga tcttgcgata
     4141 gattccccac tctgataagc catcgatccg cgcactctca cataaagaat ctgggatact
     4201 actgtaatat tgcttcaaca agaacacacc aaaagcattg aacgcttgta atagcaccaa
     4261 cgaccacaac gtatctgtta aacccacttt tctcatcata atgaactgcg ggatcatata
     4321 agactgccaa gggacagcaa tcgtgccgat atatgctaaa aataaaatat cgcgcccttt
     4381 aaaatttagt ttagaaaacc cgtaagcagc aaaactagaa gtaaacaact gaatcaacgt
     4441 gatcacaacg cttaaaaaag ctgtattctt aaaaaatgtc actaaaggaa tacgagacca
     4501 aataaccaga taattctcta aatgccaagt tttagggatc cattgaatcg gaatcgtgaa
     4561 cacttcatta tttgctttga atgaagcaga aaccatccat aaaaaaggaa tgatcgtcaa
     4621 taaagctaat acaatcagca gaatcatacc caaccattga ataaagggca tgttttttga
     4681 acgaattttt gctttttttc tttcatttcg aacacctcca aagttataat cttcatttct
     4741 ttgtaatgat tacaatccag aacaacggtt attatagctg attcatttcc gttcttctta
     4801 tttttgtaag cgcttattta acaagttatg taaatctctt tatttttttg atttaaaaat
     4861 gaaaaaatac aaaagcaaga ctgtcatacg tctcgctttt gtattgaaat ttcttcgatt
     4921 agagatactt attactgttc aaaagcttct ttgaaacggt gaatcaattc attgactaca
     4981 ggtgataaaa tccgattctt tttccagact agcatgcagt tcgtttcaat cattgggtca
     5041 agcggaacga atgtcactcg ctttgaatga tgacttacag caccttctat gactaaagca
     5101 gcacctacct gattctcaat tagcgcaaat acattgaaaa tcaaattgta cgttcctgtg
     5161 atcagcaact gctccttttt tatgcctaac cagtcagcaa acattcgctg tatttctgga
     5221 cgctttgaga tcaaaagagg tacattcgca aagtcttctt ttgttatcac gtcttttttt
     5281 gctaaaacag aattttctaa aatgagcaat ccccattttt cctttcttgg taacaaaatc
     5341 gtttcgtatt tctctgtatc tactggatct agcaaaatcg ctaaatctaa caggcttttt
     5401 tctagtcact ccgtgatatc atcacttgtt ccgctgtgga tatgaaattg aacgctggga
     5461 taatcacgga tcaacttttc tagtaatagt gccaatgtat tcgaactatc agcttccaca
     5521 cagccgatcg taaatcggcc gctgaataat tgtttttttc tgttctcgaa ttcttgagtg
     5581 gtttgattcg ttaaatctaa aatttcttct gctcggcttt tttagaaaat aacctgcttc
     5641 tgtcaagatc attttgcggt tttcgcgtac aaacagttct gttcctagtt actcttctaa
     5701 ctccttcagt tgtcggctta aggttggttg tgtaagatgt agaacttctg cggcttgaga
     5761 gattgttcct tcttctgcaa ttgtccaaaa atattttagt aagcgtaact ccatagcgat
     5821 gctccttgat ttttttactt gatacattcc attttatttc tagataacac acgatcatat
     5881 ttcttgattt ttccttgtaa acgttccaaa cattcttgca tttcagcaat ttttttcgtc
     5941 aaaagttcac tttcttcaat caaaagctta cgccgctcaa atacagttgc ttctccttcg
     6001 ctagataggc gaatatattc aatcaaagac tcgatcgata atccggcatt tcgcatacag
     6061 atcgtaaagt cgacccattt taggtcttct tctgtataat cacggattcc gccttttgtc
     6121 cgattaactg gtggaatcaa cccgattcgt tcataatagc gcaatgtatc ggccgaaatg
     6181 cccttttctt cactgacttg tttgatattc atctgatcac tctttctctt aatttcttgc
     6241 cactttactt atgtatttta tttattctac aactttaagt gtactccaag tcaatactca
     6301 tttctattaa aactaagctg gattatctat atcggttgtt tgtgtattat ttcaaataaa
     6361 aaagtcttgt ttttttattc ttttttggat atacttttaa caatttaggg aaattttagg
     6421 aaaaggggta taaaaatgac aacgtatcag cgaaaggtat tagtatcgac atcggcggga
     6481 attgctttag agaatatgga tattatgttt ttagcttttt cattatcttc aatgattact
     6541 tcttttcata ttagtggtac gcaagcgggt ttcatttcaa cgatcactaa tttggggatg
     6601 ctcattggag gtattttttt tggcatcata gctgataaat atggacgtgt gaaagtattt
     6661 tctcaaacgg tccttctgtt ttcgattgct tctttattga tgtattttgc ttcaaacatc
     6721 tacttggttt atctctttcg ctttatcgca ggtgtaggag cgggaggaga atatggggcg
     6781 tgtatgtcct tgatttccga aagtttttca aaaaaacaaa ttggacgagc ttcttcggtt
     6841 gctggtattg gtgcgcaggt cggtgcggct ttggctgcga ttttagctgc tgtgatcatt
     6901 ccaacgtttg gttggaagat gttgtatgtc gtaggtgtac tgccggtgtt gatggtcttg
     6961 ttcattcgtc gcggcttgac tgaaccaaag gaattcaaat ctgtacaatc tgccgaaaag
     7021 aaaaccaaac ttagcagact gtttgaatca aaacatcttg cttggcaaac atgtggcctt
     7081 agtatcatgg taactgtcca aattgccggc tattttgggt taatgaattg gctaccttca
     7141 attatgcaaa atcgattagg gctttcggtt tctgggtctt cattgtggat ggtcagtact
     7201 attttaggca tgtctttggg gatgatgact tttggcataa tcatggataa attggggcct
     7261 aaactagcct tttctttgtt tttaatttgt tcatccttat ctgtctatct acttgtgtta
     7321 gcccatagcc agtggagttt ggtagcagcc gctgtgatag tcggctactt tatcaatggc
     7381 atgtatggtg gatatggcgc agtgattagt agtttgtatc caacagaaat ccgcgcgacg
     7441 gcgaataatt tcgttatgaa ccttggacga gctgttggtg gtttttcttc tatcgtcatc
     7501 ggtttcttga tggatcatta tagtttaact gccgtaatct tgtttttgag cggtatttac
     7561 ctagtcagtc tgctggtctt gacaactctt actggtgtta cagcattaag ggaaagcttg
     7621 cagcattgat agttgagtct gtaaatgaca agaaagaggt gtggaaaaag cttgcatctg
     7681 cggtaataag accagccaaa tcaaaaacat gagacactgt ttccgtttgg ctggtcgtat
     7741 tacttgatgc agggcagctt tttcaactgc taatttcctt ctatctggcg gcgactattc
     7801 tttgacgtcc gtcatttcaa agtactttac taataactta gcataacgaa aaactgtgga
     7861 tacactcgta tattgagcgt ctgcataatc ttccaaattt ttctcaggat gttgatacat
     7921 gaattcaatc aaatgataat tatatgattt tctgtagaaa tgaagataaa taatgtagat
     7981 tgggaagttc tcctgctttt ccatagaaaa gcgccgatta ttctcattct tcaaaatgac
     8041 cttatcagga tattgttgga acaattcttc taaatctgtt agataattca agatagactt
     8101 acttgatacc ttcaattcgt tttgtagttc ttctattgaa caccaacgtt ctgtttcaag
     8161 cagtaaatga atcattttaa ttttcttttg agaaactttt gttaaaaaag tctgcatatt
     8221 ttctcctcct gtatctactt tactatacta catacaagaa aaaaacggat acaaatatca
     8281 tattgtcaca ccgtcaatag tgcataaacc tgttattatt tctctttttt tcctaagtat
     8341 tctattgata tgaatcattt aatcgtttat tatataaatg tcaataatct aataataaaa
     8401 taacgtacat attttattat tgcatactta ataacagaac tatagcaaaa aatcccgtgt
     8461 atcaacacgg gattagatta agtttcctca atcactattc ataacacaat tattataaca
     8521 agtaagtaaa caattgaaaa ggacatttat tgaaaacggt tgttttttac ttaaggagga
     8581 aatttagtga aaaaattagt tgcgttgttt ttggtcgcag gaagtttgtt gagtgggtgt
     8641 ggtgctacta cgtctactac tcaaagcaca agttcagctc aggcttccag cagcagtacc
     8701 acgatctccg aaagtacggc agattcatcg aaagaacaaa ttgaaaagaa aaaagaagaa
     8761 gagaaacaaa aagcggaaaa agctgcacta gaaaaaaaag tgacagaagc ggaatctgct
     8821 ttgtcacaag ctgaagcaaa tcctacaaaa gaaaactatg caactgcttc agcagctatc
     8881 caagcgctac ctgaaaacaa acaagagttg acaagccgtc ttgctgcaat tgattccaca
     8941 atcaaagcaa aagaagcaga agaagaacgg aaaaagcaag aagaaattgc acgacaacaa
     9001 gcagaacaag cgcaacaaga agcaatcgca cagcaacaag cagaacaggc gaggcaagaa
     9061 gcaatcgcac agcaacaagc agaacaggcg cgacagcaag cagcagctga acaggcttct
     9121 cagcaggcac aagccgcagc tcctgagcaa aataatgaac aaactgtcta tgtcacaccg
     9181 accggttcaa aataccatac acataaatgt ggaaatggta cgtatacccc tgccacttta
     9241 agcgaagctc aagctcgtgg tttaacccct tgctccaaat gttatggaaa ctaattcaaa
     9301 agttatacta taataataaa attcttctga aacataaaaa aatagaagct ggcgttccag
     9361 atagtaaacc aaaaatagca gtttacacgt ccatttcgtc agcttctctc ttttttcatt
     9421 tagtctgttc cctctgcttt tttggacatc ttcattcatt ctttctcaat caaatagtac
     9481 acctcttttt cttttcgcag tttatatctt ccttttttca atttcattga aaactgtttc
     9541 gaatgtttat gttttacatg gattttaagc aggagctcct catgttgcaa gcgatagttc
     9601 ctgcctttta aatcagaaaa tctcactagt ccgtattcat cagaagcttt cacaaatgga
     9661 actttttctc tagtaatata gtgtttgccg ttgagcgttt ttacagaaaa gaccattcct
     9721 tcgattggtc gtccgtttgt gttctttaca taaaaagata aaggatagtt tcgcttagcg
     9781 atggccaaag aatctaaata agcgatgagt agccaaacta agccgattga tccaagtagt
     9841 ataccgataa accaaataaa aaatttccat tttttccatt gatcaattcc ttggatcgct
     9901 ttttttgcct cttttgcttg ataaggaata cgatgccccg tcacaagcag tcgatgtgta
     9961 ttgaccatat aaggagtgca ggtaagcaaa gtcacgagat ctctgccttt ttggataccc
    10021 aaagaatcta cttcgtcagg taagaccact tggatttttt ctacttgata cgccaatgtc
    10081 tttccattga tctggatgaa aaactggtct ccttttttca acttaggcaa atctgtaaat
    10141 aattttgctt ctgggagtcc acgatgccca gaaagaacgg catgggtgtt tttcccgcca
    10201 gtcggataag aacttccttc caataatgac gttccttttt gcaaaaatat ttccgttgtc
    10261 tgatcgaata taggtaagct gacattgatt tttgggattt cgatgactcc aagcatatgt
    10321 tccataaaaa aggcattact aggtaaatct tttaatgctt ttgcatcaac tgcttggttg
    10381 aaactgccta tccctggtga tacgttttgc tctgccaact gttggttttt ctttgtcatt
    10441 ttttcctgct gtttttttat ttcctctgaa ttcttattcg atgcttgctt ttgataatgg
    10501 atgatcattt gctgggcaag aaaatcattc agtgaatctt ctacaaaagg ataagaaaag
    10561 atcccgatcc caacgactaa aatgattgcc atgaagatgt ccagtattct tattctgctt
    10621 ttattcatca tgtccaccta ttttctaata tgtatatgaa gaggttgtga aatcagcgcc
    10681 ttgacctgag tattagcgaa gcaaacggca aaatagttcc tcctattttg ccgtttgttg
    10741 agcgaatacc aagagaccga ccacttgtca caacctttct ccccatttga atcactatcg
    10801 tttatttatc gtatgatttt tgtttccaat agtttaagct tctgtttctt gatggcgttt
    10861 tttgaaatag atcactgctc ctccaactaa agcagtacct accaagatga atacgatgat
    10921 ccccatacca ccagttgaag gcaagattcc tttaggttgg ttatcgatcg ttgtttcatc
    10981 agaagttgtt ggatcatcat ctactgtaaa ttcagtgtac gcagcttcat tcttaggtaa
    11041 tgcgtagcct tctggtgctt ttgtttcttg aaggcagtat gccccttgtt tcagcccttc
    11101 gattgaaaat ttcccttctg aatcagaaat caatttcact tcttcccagt cagaacctgt
    11161 gacttcatct gaccaagcat atgggtattc tcctgaggca aggtttgcta tttttttcat
    11221 taatttaaca gttggatctt ttgctcttag aatagaaaat tctgcatcgg ctaatttttc
    11281 ttgcgtgttc acatcttgtt tgatgaagtg gaaatcaccg tagttttgga cattttctac
    11341 agtaatcgtg ttatccgtag ttgtattttc ccgattgact acaccgtttg gattcccaag
    11401 attggcttca tctgcgccat ggatgatacc ggttgtttgt tgcaattttc cgttttttac
    11461 tataaaagta gtttcttttt gtggattggt taattgataa ccatctggtg cttttgtttc
    11521 aactaaagta taattgccgt ctattaatcc attgacactg atggatcctt cagtatcagt
    11581 tgtgaatgtc aacgctgact cagcaggatc atagagagcg acaccagtgt tttcatcaaa
    11641 accggtcacg aattgattct ctgaattttt gatctggaat tgcgcccctg ctaacttatc
    11701 tacccctgaa aagttcccag ctttctggat tttgatattg ctttctttga tgacattttt
    11761 agcatatagg tgaatcgtgt ctaaaggttt gtcagggtct agggtaagct taacacgata
    11821 tttggcgacg tttcgacacc gggctttggt gtttctacaa agacgtatac agcatctttc
    11881 ccatcagatt tacctggaag agtcaggtta gctaatcctt caccagaagt cgttacactt
    11941 gcgaagggtg tagcgtctgc tggaactgct gtgtctttac ttggatagta cgtagcagtg
    12001 atgtcatata cgttgaattc aacgccgttc aacggttcgc tattcggaaa tgtcaattcc
    12061 gttccgttgt ttatttgatt ttctggtgct gaagtgaatt tcaatttatg caaattaacc
    12121 gtaacatttt ctggtgtagg tgatacggct gcagaaacct ctgcagttcc taggccgaga
    12181 acgaaaggaa gcagcatgat agcagttcct aaaaacgtcc atatttttgt tttgatgttt
    12241 ttcattttat ttccctccta ttgtttcctt tttgttgttt tttataaaac caagctgtcc
    12301 ctgttgaaag gactagcaat aaccctaaaa ttgagaaacc tccaagcatc tcttcgcttg
    12361 tttttggaaa gcttcctcca tttttttcat ttgatggacc atttgtctgc atctgaactg
    12421 gcgtttttcc attattcgtc ggatttgctt ttggttcgtt gatgatttcc aatggtatag
    12481 gatgaagcgg atcataactg cctttttcaa cttcaaagag aatggttgat tgactcaata
    12541 tatatccttt aggcggttta atctccacta gttcgtaact tccaaaagac aatccatgga
    12601 cagaaaatgt tccatcttta ctggaagtaa aaatcgttgc tcttgaatca tctttggcta
    12661 cccaatgatt tatcccgttt tcttgcgcta agtattggtc attttttttg actagaaatt
    12721 gagcatctgc tagtttttgt ttcgtttgcc gatccacttt gacaaattgt ttgcctcctg
    12781 tttggatggc aagatgtgtt tctactgttt gattgtcagt cgtcaaacgt acatcgttat
    12841 caaatgtcgt tttttcactg ttttctttca atgtcatttg gtaacggatc gttaattttt
    12901 ttccagcaaa cgaactaaga tcttttccag aatctgataa gacaaaacca tgttttattt
    12961 tttgtgtgtg aaatgtatga atcttgtctc caccaatcaa aaaatcgatc gtttctggtt
    13021 ctagccataa ctgaggatca gctgtatctt tgatcgtata ggttcctatc tcgttgatgt
    13081 tctcaggaat tgttacggtg atgagataag ggactttatc cccatctgca aaatctgtct
    13141 gcttattctc tactgttttt gttaatgtga gtgcagcagg tattttttgt tcgctctttg
    13201 gatacaagtg gatggtcgtc aatttatgat tagatgaatc tgtcacagga agcacaacga
    13261 caaatggtgc tgatttttct tttacttaat ctgaagtctt tatttccgta aatcggtaaa
    13321 cagcgtcacg accttgctca tctttgtccg atgcactaaa agaagcaatg ccctcttcct
    13381 cgtctactgt ttttgtcaca ttttctgcca gtattttttc actatcactt ttttgtgcta
    13441 gttttctttg tgcgtcttcc acactacttc cttctgaccg cagcttgtaa aattcatccg
    13501 agacatcata tatagcgaaa gtaacgccgt tcaacccttt tactccttga aagttatccg
    13561 ggatatttat tccgtcgttt tgactgatac ttggcatctt atcgctttcg aacattcgat
    13621 tatgcaaagt aaatgtaata ggtgattctc tagctgacac agtcggagaa cctataaaaa
    13681 agaccagtag gataaaaaag agaacagcaa atatatccac acgtacattt tttgtttttt
    13741 tcatcgctgt tcacctgctt ttttcttacg atggataaaa taaaagaagc cagaaaaaat
    13801 aaagatcagt cctaaaaaga caaagggcag tagacctatc ccacctgtcg aaggaagcaa
    13861 gagagtcgga ttgatcgtca acttagcggt atctgttgcg ataatcgtat aattattgtt
    13921 tacacttcct tcgcctaaac taatcgttac tatttgtgat ccaatatctg tccggctgtt
    13981 aactgttagg gtataatcca atgattcgcc taagacaaca ccggtaacgt ggagatttcc
    14041 aaccgaaatc gagtgacttt gggcatcata attaaaggca gcgtcatcga cagtaatcac
    14101 tgcgttagct ttatagatgg tataagttcc tgatgtgttg gctatatcag taaatgacaa
    14161 cttgggattt gctgctttga ctttgtctat tccgctattc gttagtttaa ttgtatagct
    14221 gccagcatta accatatttt ttgctgattg tgcatctgct gtgtaatcca tactgctaag
    14281 tgccacttgt actggagtaa gcgaactagt gacaccctct gctagcttga catggattga
    14341 cgggacacct gcttttccat catatgtctt actgccactg agctctaccg caccgctgat
    14401 ttgcttaatc gtatagacat cagatgtctg cacgatagta aataaatagt ttgttctggc
    14461 agctagttta gctagtccgc tttttgtcaa ttgccaagac gtataggttc caatttcagt
    14521 aggcgcagtt acttcaactc ccttttcatt catcgctttg aaatcagttg gattccatgt
    14581 gatttgatca atactgagat cattgtttaa aagggatgtt gattctgttt tatcattaaa
    14641 ggcatattta acgatcggta caacagaaag accatcatag tacttactgc catttgcatt
    14701 gattgatact gttgcatttg tcttataata taggatgact tcagaaatct gttttccatt
    14761 tgcgtccttt acgattacat ttccatttgt atctaaacta gctaaaacac tcattgctga
    14821 ttggttcttt gtttctatcg ctgaaacagc tgtaaaggta tatccgttga tagtaggcgt
    14881 attagtcgtc aggtcttgtg cagcacttcc gtaagtaaga ttcattattt tagaaggagc
    14941 aatcatctgt ccaaaatagt cttgatactt aatagtgatt ggtgtttgtt tgcctgtata
    15001 ggtcaccgta tcgacgttac tttctagggc tgtgaaagaa ccgtttgttt gtgcaaaaga
    15061 atagcccgtt actgctgttg gagtgataac cactttgtca cccacatgga ttccagataa
    15121 tgtagtcccg tacgttccga tttttgctgc tccaccattg actgtataag aaaaagttcc
    15181 tgtccgattc gcattgacct gaaccgttgc tgcttgatag gttggaaaat ttgaaacatt
    15241 tcccttacca tcaacgatct gcccattttt gatcgagtac cccgtgaatg gaatatcatt
    15301 tgctaactgt ataccgattg aaaaagtact catacctcct ccggcaggaa gccctcccca
    15361 tgttgagggt gtaaaagaat aacttttatc cgcattgggt gtaactgaat cgatcgtatt
    15421 tccagcggta gccaccccca tatagatact ttttgggaat gtgatagtag taccatttac
    15481 tgtggagcca ataaacagat tatggatatc tggacggtta tcaataaaag cgattgcttt
    15541 ggttacgcct tgtaacgcag ttttatccgc actcatcgat ccgttaaccg caacaatgac
    15601 tccatctttg gcatatatca ctgtatatcc atctggtatg gtatgtccgt tgaaattcgc
    15661 agtggccgca acagtactaa atgtctggct tctacctgtt tctgaataag ctttctcccc
    15721 tgctgctttt gctgctgtga tttctttttc tgtcggatca atcagttgag tcgaggatgt
    15781 ggttgaggtt gtctcgttgt tgctagactg attggtgctt tctatggact gtatttcaga
    15841 atagggtgga ctatttgtct cctctgtcgt ttggttagca aaatttgacg tttctgtata
    15901 aacgtttaca taaatacttg ataagctccc taagaaagta aaaatcaaga tgatcgaaat
    15961 cataatggga tgtaagtatt ttcttttaga tacagctttg agtctagaaa caaaatgttt
    16021 ttgacatata cgttggattt tattttgagt tttcttaatc attttcatcc ctcctctcaa
    16081 taaaaaatca tcaaataatc ctttacacgt tattctaaat ttaattttta ttattttatt
    16141 gatgtataga aagtttgggg ctttttttct ctttttattc taaggttatt ttagt
//
LOCUS       DAZULO010000065        15954 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000065 DAZULO010000000
VERSION     DAZULO010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.066"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(390..812)
                     /locus_tag="ACG8OA_001971"
     CDS             complement(390..812)
                     /locus_tag="ACG8OA_001971"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFS4976737.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(826..2232)
                     /gene="atpD"
                     /locus_tag="ACG8OA_001972"
     CDS             complement(826..2232)
                     /gene="atpD"
                     /locus_tag="ACG8OA_001972"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFS4976738.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2255..3157)
                     /locus_tag="ACG8OA_001973"
     CDS             complement(2255..3157)
                     /locus_tag="ACG8OA_001973"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFS4976739.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3171..4727)
                     /gene="atpA"
                     /locus_tag="ACG8OA_001974"
     CDS             complement(3171..4727)
                     /gene="atpA"
                     /locus_tag="ACG8OA_001974"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFS4976740.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4750..5292)
                     /locus_tag="ACG8OA_001975"
     CDS             complement(4750..5292)
                     /locus_tag="ACG8OA_001975"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFS4976741.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5279..5803)
                     /gene="atpF"
                     /locus_tag="ACG8OA_001976"
     CDS             complement(5279..5803)
                     /gene="atpF"
                     /locus_tag="ACG8OA_001976"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFS4976742.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5894..6109)
                     /gene="atpE"
                     /locus_tag="ACG8OA_001977"
     CDS             complement(5894..6109)
                     /gene="atpE"
                     /locus_tag="ACG8OA_001977"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFS4976743.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6162..6881)
                     /gene="atpB"
                     /locus_tag="ACG8OA_001978"
     CDS             complement(6162..6881)
                     /gene="atpB"
                     /locus_tag="ACG8OA_001978"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFS4976744.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7183..7647)
                     /gene="smpB"
                     /locus_tag="ACG8OA_001979"
     CDS             complement(7183..7647)
                     /gene="smpB"
                     /locus_tag="ACG8OA_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFS4976745.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            complement(7669..10026)
                     /gene="rnr"
                     /locus_tag="ACG8OA_001980"
     CDS             complement(7669..10026)
                     /gene="rnr"
                     /locus_tag="ACG8OA_001980"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFS4976746.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            complement(10029..10784)
                     /locus_tag="ACG8OA_001981"
     CDS             complement(10029..10784)
                     /locus_tag="ACG8OA_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4976747.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10887..11123)
                     /gene="secG"
                     /locus_tag="ACG8OA_001982"
     CDS             complement(10887..11123)
                     /gene="secG"
                     /locus_tag="ACG8OA_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFS4976748.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11241..11519)
                     /locus_tag="ACG8OA_001983"
     CDS             complement(11241..11519)
                     /locus_tag="ACG8OA_001983"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4976749.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11632..12600)
                     /locus_tag="ACG8OA_001984"
     CDS             complement(11632..12600)
                     /locus_tag="ACG8OA_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4976750.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12815..12859
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12883..13857
                     /locus_tag="ACG8OA_001985"
     CDS             12883..13857
                     /locus_tag="ACG8OA_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFS4976751.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13862..14437
                     /locus_tag="ACG8OA_001986"
     CDS             13862..14437
                     /locus_tag="ACG8OA_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFS4976752.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14504..15643)
                     /locus_tag="ACG8OA_001987"
     CDS             complement(14504..15643)
                     /locus_tag="ACG8OA_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFS4976753.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4359 a         3443 c         2856 g         5296 t
ORIGIN      
        1 aatctgagcc agaatgtggt tcattctgac tcagatttga ttttgcacaa ctcatcagtc
       61 cttattgaca aagagcccaa aagtcttgtc gcagtccctt attccaaata aacggtgttc
      121 aatcatctga ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac tattttccat
      181 ttgtttctct aatactcagg acaaaacgct gatttcacaa cctccgatat acggtgttcc
      241 agaagtagct tcttcgaaaa ataagctgag atttcacaaa aatttgagaa gcaattttcg
      301 taaattcctt cttatgtctt gaagctgacc acttctgtca taacctcttt ttactgttct
      361 taaacacgga taaacatccg catctcattt tatgagtgct tagatacttt gatacgattg
      421 attgctcggt gcaacgcaac agttgcccgt ttcaattcgt tcgtatcttg ttttgctttt
      481 gcttcttcga tttggcgttc tgcgcgttgt tttgcacgtt ccgcacgaga tacatcgata
      541 tcacgttcac gttccgcact atcagcaacg attgaaacaa cattatcacg aacttccatg
      601 attccgccat taacagcgat ccaatcgacg tgggtatctg aatctgttcg ttttacacga
      661 acttcatcaa tcgtcaatgg aacgatgatt ggtgcatgtt taggcaaaat cccaagctca
      721 ccagctgtcg tacgtgcaac gacgatagct gcatggtgat catagaccaa accggcagga
      781 gttaccacat ttactgttaa atattgatcc atggggcacc cctttctagt agcctagtgt
      841 tttcgctttt tctacgacgt cttcgattcg gccaacactt cggaaagctt cttcaggaag
      901 atcatcgtat ttgccttcca agatttcgcg gaatcctttc actgtttctt ctacagggac
      961 atatgaacct ggaagaccag taaattgttc agccacgtta aagttttgtg acaagaagaa
     1021 ttggactctg cgggcacgag aaactaacac tttttcttga tcggataatt catccatacc
     1081 taagatagca atgatatctt gtaattcacg atatcgttgc aatacgtgtt ggacttctgt
     1141 tgctacttta taatgttctt ctcccacgat ttcaggtgct aaagcacttg aagaagaagc
     1201 caatggatct accgctggat aaatcccttg ttctgttaat ttacgttcca agttggttgt
     1261 cgcatccaaa tgggcgaatg ctgttgccgg cgccgggtca gtgtagtcat cggctggcac
     1321 atagattgct tggatcgatg tgatcgaacc ttttttcgta gatgtgatac gttcttgcaa
     1381 ttgacccatt tctgttgcca atgttggttg ataaccaacc gcagaaggca tccgtcctaa
     1441 caaggctgat acttcagatc cagcttgagt gaaacggaaa atgttgtcga taaacaacag
     1501 tacatcttga ccttctacat cacggaaata ttccgcaatc gtcaaaccag tcaaagcaac
     1561 acgcatacgt gcacctggtg gttcgttcat ttgtccgaac accatggctg ttttttcgat
     1621 aactcctgaa tctttcattt cataatacaa gtcattacct tcacgagtac gttcacctac
     1681 accggtaaat acagaaatac caccatgttc ttgggcgata ttatggatca gttcttggat
     1741 cagtacggtt ttaccaacac cggcaccacc gaatagtccg actttcccac cttttaaata
     1801 tggggcaagc aaatcgataa ctttgatccc tgtttccaaa atttctgtac ttgtacttaa
     1861 ttcgtcaaag gctggtgctt ttttatgaat ttcgcttctt tccgcatctt ctgggaaagg
     1921 tgtttctaaa tcgatcgtat ctcctagtac gttgaatacg cgacctaatg tttctttacc
     1981 tacaggaaca gagattgctt tgcctgtatc aatgacttcc attcctcttt gtaaaccatc
     2041 tgttgattcc atcgcaatgg tccggatcac tccgtcccct aattctaacg ctgcttcaag
     2101 aacaactttt gatttatttt cgtcattttt ataaacaact aatgcgttgt tgatatctgg
     2161 taaggattgg tctaaagaaa attccacgtc gacaacggga ccgattactt gaacaatctt
     2221 gcctgaactc atcttttttc ctccaatctc aatactattc tagtgccgaa gctccggcaa
     2281 caatttcggt aatttcttgg gtaatagccc cttgtcttgc acggttgtaa gaaaccgtca
     2341 gctcatcaat gatattagca gcattatcag ttgctgtttt cattgctgtc ataccagcag
     2401 catgttctgc tgttttggcg tcaacgatcg caccgtagat caaactttca gcatactgag
     2461 gcaataactg agacaagatt tcttcttttg aaggttcaaa aatatattct tgttcgaaag
     2521 tagttgcttc ttctggatct aagtctgaga ttggcagcat tttttctaca cgaaactgac
     2581 tagttagcga attgatatga tggttataac atacgtataa ttcatcgaat acttcgtttt
     2641 ggtacatagt tgttgccata tttacgattt tgcggacttc atcaaagctt ggctgatcag
     2701 atagattgcg cagctcatat gctagattga taccgcgtgc tttaaagaag tctgcaccag
     2761 ttccgccaat tgcgatcatc acgtaatcat cgggtgattg gtggtcttct tctaaaatag
     2821 acatcgtttg tttcaatatc gagctgttat atccaccgac taaaccgcca tctgcagtga
     2881 tgacgatata acccgttttc ttaacaggac ggctgagcaa catgctgttg taattgatat
     2941 cccctctagg attttctgag gcaatatcat ttaactgagt agcagtcaaa tgcgtaacga
     3001 tttctcttac ttttgttgca taaacctgaa acttctgtga agaagcttca gattttgtca
     3061 acttagaagc agacaccatc tgcattgccc gcgtgatttg gctcgttttt ttcgttgaag
     3121 cgatacgtgt cttgatttca tttaatgaag cacccattca tctcacctca ctatgcattt
     3181 tgaatggatt tcaatttatc ttctgtagag gaattatttc cttttgtagc taagaacatg
     3241 tctttatatt cctgaatcgc actgttcaac gcttcttctt ctggaagatc ttttgttgta
     3301 cggattgttt caaagatatc tgaatgattg gtatccagat attcgaataa ttcatgttcg
     3361 aagtctaaaa tactgtctac agggatacta tctaagaatc catgtgtcaa agcatacaaa
     3421 atcactactt gtttttcaac agctagcggt gcatgcaatt tttgtttcaa gatttctact
     3481 gtacggcgtc cacgatttag ttttgcttgt gttgctgcat ccaagtcaga accgaattgt
     3541 gtaaatgctt ctaattcacg gtaacttgct aagtctagac gtaaagtccc agcaactttt
     3601 ttcattgctt tgatttgcgc agaaccccct acacgagata cagatagtcc ggcatcaacc
     3661 gctggacgag tacctgcgta gaacaagtcg ctttctaaga agatttgtcc atcagtgatc
     3721 gaaataacgt ttgttgggat ataagcagag atatctccag cttgtgtttc aacaaatggt
     3781 aaggcagtca ttgaaccgcc acctaaatca tcacttaatt ttgctgcacg ttccaataag
     3841 cgtgagtgta agtagaaaac atcccctgga taagcttcac gacctggtgg acggcgaagc
     3901 agcaaggaaa gttcacgata agcaacagct tgttttgaaa gatcatcaaa aatgatcaag
     3961 acatgtttcc cgttgtacat gaactcttca cccattgcag taccagcata tggtgcgata
     4021 taaagcaacg gcgctggttg agacgcaccg gcattcacaa cgattgtgta atccattgcg
     4081 ccatattttt tcaatgtttc aacttgtgta cgaactgtag aatctttttg tccgattgct
     4141 acatagatac agatcatatc ttgacctttt tggttgatga tcgtatcgat cgcaatggaa
     4201 gttttccctg ttttacggtc accgataact aattcacgtt ggccgcgtcc gattggtaca
     4261 agagcgtcga ttgcttttag acctgtttgc attggttcgt taacagattt acgttgcata
     4321 acgcctggtg ccattgcttc tactggacga gctttgtcag taacgatttc acctagtccg
     4381 tcaattggct gacctagcgg attaactacc cgtccaatca aggcctctcc aactggaact
     4441 tccatgatct ttcctgttcg ttttacttta tctccttcac gaatggactc gaaatcgcca
     4501 aggataatga tacctacatc atttgattct aagttttgcg ccattccgta tgagccattt
     4561 gaaaattcaa gcaactcacc actcatcgcg ttttctaaac catgtgcacg agcgatcccg
     4621 tcacctacat aagttacggt accgatttct tcgactgaaa gcacgttttg ataattttca
     4681 atttgttctt taatcaaggc actaatttct tctgctttga tggccattcg attcacctac
     4741 ctctttgtac tatctattta attgattacg catattttct aattgcgtgc gaatgcttcc
     4801 gtcgattact cgatggttag cttcaacaat cgcgccacca ataatagaag aatcaacgat
     4861 ttgttttaat tcgactgatt gatagttcat tgtttttgca acattctttt ctaattgtat
     4921 tcgttgttcg tctgaaagtg gaactgctgt tgtgacactt cccaacagca aaccttgatg
     4981 ttcatcatat cgacgttcat attcttcgat catgaacaaa agatcatcca ttcggttata
     5041 cgcataaaca acttctaaaa agtttttaac gattccatca tagccactaa caagcttgtc
     5101 catgatttct cgtttttcat gaggttctag acgaacatca cttaacatat tacctaatcc
     5161 aggtacttgt tcatagattt gacgtaagct tacgagatct tgataaattt cttctgcttt
     5221 atttgaatcg atcgccagtt caaataaagc tttcccgtaa cgtcttccta ctgtatattt
     5281 atctagtttc atttgaagaa cctagacctt cgatatattg attgattaat gattcgtgca
     5341 tttctggaga taattctttg ttcaagattt tttccgcgat ttgaagagat agttctgcaa
     5401 catcgtcttt tacagagttc agcgctgaat cacgttctac agtgatatca gcttgggctt
     5461 tactttttag acgagctgct tcttcttgag catccttcaa aatattttgg cggcttaatt
     5521 ctccactttc tttcgcattt ttgatgatat cagctgcatc agaacgagag gctaacaatt
     5581 gttgttcgcg ttcttgttcc attttcgctg agttgatgcg agattgttct gcagaatcca
     5641 aatcattggc gatcttgtct tcacgttttt tcaaaatatc gctgattggt ccccaagcaa
     5701 aatgcttcaa gagagccagt agtatcaaaa atgagccgct gacaacgatg acatttccta
     5761 acatcgcatt gccaacttct gcaattgcca attgattcag cataggcgag ccttcctttc
     5821 tttgaagtac ttctagtttt catgtaaaaa agcagtaaca aaccaaattg tttctgcctt
     5881 aatactgatt caattaaaca ccaaaaacta gaatcaaggc gacaactacc cctaagatag
     5941 gaacagcttc gactagagct accccgataa acattgttgt acggaattga ccagacattt
     6001 caggttggcg agccattgat tcgattgttt tagagataac ttgaccgtta ccataaccgg
     6061 ctccgattgc tgctcccatg attgcgattg ctgctgcgat ataattcata attgttttcc
     6121 tcctaaatta gataaatagt agtttgtttt taaaacgaat attattcttc cacttcaatc
     6181 ttatggctca tataaaccat agataaagta acgaagacaa atgcttggat gcttccgata
     6241 aacaaggaga atgcgatcca gaccatctct aatggaattg ctaacggcaa tgagaaccag
     6301 cctaagctta ttaccatttt tgcaatcaag gttagcaaaa cttcccccgc ataaatattc
     6361 ccataaagac gtaaagcaag tgttaataag tttgtaaatt cttcaatcag tttcatcgga
     6421 aacataaaac tatatggttt caagaacgaa tttacgaaat atcctttgaa gcctaacttt
     6481 ttcaccccaa ataagtgagt aagtaagatc attataaatg ccaaagttaa agtaacaaac
     6541 gggtctgctg tcgggctttt ccaaagtgtt gtttcgttag gtaaaacgat ttttgttact
     6601 aaaccgatgt tgtttgcaac aaaaacaaat aaaaacaatg taaatgccaa caaatggaaa
     6661 ttgctcaatt cttttctagg cagattatct gttacgattc ctcgactaaa atcaatgacc
     6721 cattctatcg cattttgttt gcctgttggt ttcaacttaa gatttcttgt aaaaaagtaa
     6781 gctaagaaga aaacaatcaa acaggtcagt aacaccatta tacaaactgt accatcaaac
     6841 caaattggtc caatgtggaa cgttagcgag cgttcatcca atccatttca cctcttttcc
     6901 ccgaaattca cataaaatca attcatgtgc gtatctggtt ttttccaatc ggtttctaac
     6961 cacacggtat cataccacca tgaaacagaa aattcaaaac attttcatga aaattagaca
     7021 cttttttatt tcagtcaaaa acgaagcgaa tttttcttta tacacggtgc tcctgatcta
     7081 agaaaaaacc tatgataatc atgttttatc agattaaatg ctaacaaatt ttatcattaa
     7141 gtaaaagcaa aagcaccgaa tatgcgaaaa aggataatga aattatctag agaaattttt
     7201 taatgttcga tcaatttgtc gatcgatatc ttttcgtttc aaatcttccc gtttatcata
     7261 agattttttc cctttcgcca gaccaatcaa taccttggca tagccgttac gaatatagac
     7321 ttttaaagga acaacagtga ttccagtatt tttcaattca ttttcaagcc ggatgatttg
     7381 ttttttgtgt aataataact ttctcgtgcg caacggatca tgattaaaaa tatttccttg
     7441 ttcataagga ctgatatgaa cattatgcaa gaaagcttct ccgttgcgga cgcgaataaa
     7501 tccatctttt agattgatcc ggctgtttcg tatcgacttg atctcggttc cttgcaagac
     7561 catccctgct tccatcgtgt cgatgatcga ataatcatgg cgagctttct tgttttgtgc
     7621 aattaatttt ccctcgcctt ttggcatagc tgtttcctcc aactcatttt attttttctt
     7681 tttcttcttt ttaacacctt tgtaaaatgg cttcttccct tttttcttac cgaacgtttg
     7741 cttactatcg ctatatcgtt tgttcttctt gccatttctt tctgggcgtt ttcctgcttg
     7801 ttttccttcg cgttttcctg attttccatt tcgtgttttt ggccgttcca acgctgtaac
     7861 ctcttctgcg gaaacaagtt caaaatctac tgctcgttca tctggatcag ctttgactac
     7921 ttttactcgc actttctgtc cgattttaaa agtcagacca gttctttcac ctacaagagc
     7981 taaatgattt tcaacaaaat ggaagtagtc ctgtttcaat tcattgatat ggatcaatcc
     8041 ctctactgta ttcggcagtt cgataaacag gccgaatttc acaacagagc tgatgatccc
     8101 atcgaattct tcccccactt tgtccatcat gaattctgct ttcttcatgg aatctacttc
     8161 acgttctgtt tctactgcac gacgttccat cttagaacta tgatctgcaa tttcaggaag
     8221 ttccgtagcc cacgaacttt gattgctttc acttttatct tcactgtaag aacggatcag
     8281 acgatggacg attagatcgg gatagcgtcg aataggagat gtaaagtgtg tgtagtaatc
     8341 tgccgctaag ccataatgcc caaaattgtc ttctgaatag cgtgcttgtt gcatgcttct
     8401 tagcaacata gtattgataa ctgcagcttc tggtttgtct tctactcctt cgatcacatg
     8461 ctgtaaatct tttggtgtga tcgttccttt cgttcctcgg accagaatgc ccaaagctga
     8521 agcaaaatca aagaagcgct gcattttttc ttcttttggc tgttcatgga ttcggtaaat
     8581 aaacggcaga tttaatttat tataatgttc agctaccgtt tcattggccg caagcatgaa
     8641 agattcgatc aagcgttcac caattcctct gcttcttagc tggatatcat ttgggtgtcc
     8701 ttcagcatca acaaggattt ttgcttcgcg atcctcaaaa gaaatcgcgc cacgtttgat
     8761 tcgcatcgtt tcaaggattc gatgcaattc acccatttct tggaacatag gtacgagctg
     8821 agcatagcgt tgcatcgttt cttcgtcttg atcttctaag atttgattga cagctgtgta
     8881 ggtcatccgt tctgttgttt ggatcacact tgggaaaata tcatgagaga ctacttgtcc
     8941 atttggatca atctccattt cgcaactcat ggtcagcctt ggaacatggg gattcagtga
     9001 acagatacca ttcgataaac gttgcgggat catcggaatc actcgatcag tcaaatatac
     9061 acttgtccct ctttcatatg cttctctgtc caattgactg ccttgtgtca catagtaaga
     9121 aacatctgca atgtgtactc caagaaaata attaccgtta tctaattttt taaccgtaac
     9181 tgcatcatct agatcttttg catcttctcc atcaatcgta acaatgagct gatttcttaa
     9241 atctctacgt ccagttatat ccttttcgct gatttgatct ggaacttcat ctgcttcaga
     9301 caatacatca tcaggaaatt tcgtagggat accattagcc acgacaattg agaggatatc
     9361 catgccaggg tcattcttat gaccaatcac ttgcttgatt atcccttcta aactagtcgc
     9421 ataccctttt tcagggtaat gggtcagttc gatcagcaca atacttccgt caactggacg
     9481 aactccctct gctgctgcat atactttaaa ttggttgagc tttctatctt tcgggataac
     9541 aacgccgtaa agatccgttt cttgtatttc ttcttctgaa taagcaacga attcccctgc
     9601 taactgagta gtcgcccggt ctcgaatctc gactactttc ccctctgctc cgcgatccat
     9661 atctgtattt gctcgcttca tgatatcgat tgctacggtg tctccatcca ttgcaaaacc
     9721 agtcgcttct tttgggatat agatgtcatc ttcttcggga tcaatcgtta caaatccaaa
     9781 tcctcgttca tttgctcgaa atgttccttc gatcaagatt ggttttaaag gaagtttcac
     9841 ttttcctttt ttattgaaaa ctatactctg ttctctttcc atttgagcaa tcgtttgtac
     9901 tagtaattta aaatcatcgc ttttttggaa ccctaaacct tcagcgattt cttccataga
     9961 aaaactgcgt ttgcgactgc tttccatgaa aaagagaatt ttctctttta tcgtttcttt
    10021 tgtcatattt attcctcatt ccaaggtaat ttttctaaaa attccaagac gtctttttct
    10081 aattcgcgtc ttgctgtacc aactgtaatc acatgaccac tttcaggata ccatcgaagt
    10141 gtcactcgct gacggctcaa cttgcgtgca gtttcaaata cgccattagg gtcgatcatt
    10201 tcatctttcc cagcttgagc taaaaatatc ggctgttgga tatctgacaa cctactttct
    10261 acgattgctg cctgatcttg tatatccatt aattgttgtt cagctaacga gcggtaagca
    10321 actgcttttt cattgatttc tttctctgat ttacctgctg tcttcagcac ttgacgtacg
    10381 tatttttcaa agttttcagg cacgtttgtt tttacaggag acaaaggcga acagaaaaaa
    10441 ccgccgccaa tcactgattc ttcttctagt gctcgtacag caaagatgcc gcccattgat
    10501 aatcctaata cggcaatatc agaaaactgc tcagactgta aaaaatggat ggctttttta
    10561 gtgtctgccc accagctttc tgctttttct tgcaaaatat catatggctc cattgttccg
    10621 tgtcctttaa aaagcggcgc ataaactgta taatctgctt tttccaaaaa tcgtgcgagc
    10681 attcgaacat cgtttggact tcctgagtaa gcgtgaagca gcaaaaccgc tcgctttcca
    10741 tgttttgcat aaaggggttt tggtaattgt ctcactcgtc tcacctcttt cgcctcattt
    10801 taccatagtt tctttcataa cttcttctta agcgttctct ttgcaataaa aaaagccgcc
    10861 cgccgttttg cgagaggctt attcaactac ttcgaagata aatatgctag gacaaataat
    10921 aagatcatcc agatagctcc cataactgca gttgatcgct gcatgacagc ttcaaagccg
    10981 cgagcttttt gtttgccgaa tagttgatcc gcaccgccgg taaatgcact cgctgcacta
    11041 ttttgcttgc ttggctgcat cataatcgca atgaccataa caatcgataa aacgatcacg
    11101 attcccaaaa ttaaattata catatcgcag cctccttccc aaaagatatc gtctctttaa
    11161 ctttatcata gataaaagtc cgtgacaact ctatataaat ggaataacag ctgaaagttc
    11221 aaaactttca gctgttattc ttatgactga cgtaagacgt cttgatattc tggtttcttt
    11281 tcaaactctt tttttgcata agggcataaa ggaatgatct ttttcccgtc tcgtcttgct
    11341 aattctactc cagtatacac tagcttttca gctaactttt gaccacgata tgctggatct
    11401 acaaatgtat ggtcaatgat catgatgtct gggcccgcat ctgaccatgt catttctccg
    11461 atctcttgtt gttcatcatt gaataaagca agacgatttt tttcttcttt gatttccata
    11521 tgccgcactc ctcaaataac tgatattttt ttattcctac cttattttag cagtctcctg
    11581 atctataaac aattatttat cctcgaatta cttacaaaaa agaagagaaa attaattcat
    11641 cgttattctt tgttcaataa atgaaaaaat cactgtccaa taggttttcg gatctttcat
    11701 cgatgcttcc aagtgttctg cttctggaac aagcagcagt gctttatccc ctgccgtagc
    11761 attttgaatc gtgtacgctg cttctgtagg gacaaaatga tctctttcac catgaatgac
    11821 caataaaggc agatggttgc tgccaagctg tctcgtagct gatgcttgct ttaaggagta
    11881 accggcgtat ttctgggcat aatgattcgc atatctcatg accatttttt taggaaagac
    11941 agtgatttta cttaacatat atcggaattc tgcataaaca gaaacatatc cgctgtctaa
    12001 aataaatcct ttgaccgctg atggaagttt ttcaccactt gccatcatga tcgtagaagc
    12061 tcccattgat ccgccgtgta aaataatgga cgcattcggc tgttcatcta agatgagttg
    12121 gatccaggca attaaatcta gccgatctag ccatcccatt ccgatgattt ctccttcact
    12181 ttctccgtgc gcacgtaaat caggtacaag aacgttgaat cctttctctg catatctttt
    12241 acctatatga gacatgtctc ttttccctgt gcttcggtaa tcatgtacgc aaatgaccca
    12301 tttattctgc ttatttggat ggatgaacat ctgaccagct agtttcaacc cgtcataact
    12361 tttgattgtt ttgggtgttc cctgctccca aaagctttca cactcttctt ctaatttttg
    12421 tcgttttttt tctactgacg tgactgcagc agggtccatc aaacgatgac cagtgtcttc
    12481 ataccagtca tatcctctct gtactcccgc tcttactaat ttctttgcat aaaaataaga
    12541 aagaaaatgg taactgatcc ctataattat cagaataaga atgaaataaa aaataaccat
    12601 tgtacacccg ccgctttctg ccaaactata atctaactat tatccaatgt accgatccat
    12661 aggattcttt attttcggca cacgtaaatt ccatttcttt ttcattgtat catttactta
    12721 aagttctcct caatagattt acttgtaaga gagcatttac attcgcaata aaagccagta
    12781 tactaatttc gtgcattata atcaagacgc tcgtcttgga ctggttcgga aacttcccag
    12841 aataaaaaac taagtatctc taaagaaagg agattggaaa taatgcgaaa agtaattatt
    12901 gattgcgatc cagggatcga cgacacttta gctttatcat tagctgtcaa atcccctgat
    12961 atcgaagtag ttgctattac cgtagtttgt gggaatgtcc ctgcagatat aggtacgcag
    13021 aatgtgctga aatgcctgga acgctgtgat cgtttagaca ttcctgttta tcaaggaatg
    13081 gaaaaacctt tgaagcaacc tttcatcagt gcacaagaca ctcatggtat ggatggttta
    13141 ggggaaacaa atttcccgat gatcttgaga aaacaggcag aacctctcca tgccgttgat
    13201 tttttggctg attatttcaa agaaaagaca aatacatcag tcattgcttt gggtcctttg
    13261 accaatatcg cttttgcttt gaaagtcaac ccaaatattg ggaaacatat ggatcgtttt
    13321 gtctcgatgg gtggaactta caaaagccat ggtaactgtt cgccagttgc tgaatacaat
    13381 tactggtgtg atcctgaagc agcttcttat gtattcgaga atctcaaaca aacgatagaa
    13441 atggttgggt tagatgtgac aagagaaatc gtcttgacac cgactatcct cgaatactgc
    13501 tgtcaaatga atcctgaaga gggagagtat ctaaaagcga tcactcgttt ttattttgac
    13561 ttccactgga aacaagaaag aattttagga tgtgtcatca atgatccgtt agctgttgct
    13621 tattttatcg agggggccat ctgtgaaggt ttcaaaagct tcactgctgt ggaaactcaa
    13681 ggaataagtc gtggacaaac attggttgat cgttatgaat tttggcagaa acctgctaac
    13741 agtaaaatta tgacaaaagt agatactcgt ctttttttcc gaaagttttt aaccgttctt
    13801 ttaaatgcac aagaagaaac gattatgaaa gatttggaaa ggttaaagat gggctaacaa
    13861 aatgaataaa aacaaaatgg caactcgcgt acttacagtg atggctttgt gtattgggat
    13921 caattatatt ggaggaacga ttgctttatg gcttcgtttg ccaatctatc tcgattctat
    13981 cggaacgatt tttgctggtg cattgcttgg gcctgtacct ggaatgctga caggattgtc
    14041 cagcggtatt ttaagtggag tgactacaga tattttttct ctgtattatt cgccaatcca
    14101 aattctcaca ggcttgcttg ctggattgat tttgccaaga tatctcggca aagataaaaa
    14161 cagcttgaaa ctgttattac tcgctttgat tttgtcggta cctggaacga tcgtttcttc
    14221 gatcattacc gttcagttat ttggcgggat cacctcctca ggatccagta tgatcgttca
    14281 gctattacat gggttaggtt taagtcagac aacaagtgtg atcttggtac aggcaggtac
    14341 agactacctt gatcgtttgt tgtctatcgt cgttgtttca ttagtcatca ctatgttgcc
    14401 acaacgcttg atatattcaa aaagaaaaat aggctgaata aacagagctg gattagcaaa
    14461 ataatgctaa tctggctctg ttttttcatt tattaaaaaa atcttacgct aagtaggata
    14521 aaataattcc ttgatatttt tcaatcatat ccaaataatt atcgatttca acatactcat
    14581 ctacttgatg aggcaaagta acgacaccag gcccaaagac gacaaagtcg aaggaatggt
    14641 tagctttcgt aaattcggct gcatccgttg ttgctgccgc tccaactaaa ggtaatggct
    14701 ggctgaattg ttgctgaatg caatgaatca aaggagaatc tggatcagct ttgacaggta
    14761 ttttgttgta atcgatcatc aattccaaat gatagtctgt ctcttgattc aattcattga
    14821 cgattgattg cagcaatgca atgattttat catttgggta ttctggaatc gatcgaatgt
    14881 tcccttggag ctgcgcatgg cttgggatgc tgttgacttg attcccgcca ctgatcaatg
    14941 taacattatg gattgttttt cctaggacag gattctcgat tgtttcagcg aggtgattca
    15001 tttcagcatt tgctttcgta atgaattcat ttaggtgatt gattgcatta taaccttggt
    15061 ctggcataga gctatgcgct tcttttccgt gcgaggtgac tgtatagttg attgatccca
    15121 tgtgcgtata catcaaactg taattagttg gctctccgat gatcaaggca tctaagtcat
    15181 ctacatagcc tgctttcgtg agctgttccc cacctagttc acccacttct tctccgacag
    15241 tggctaataa tttgactgtt ccgttaaatg gttttcctga ctctttcagt tcgatcatgg
    15301 caatcaccat tgctgccaag ccactcttca tatctgtagc accacgtccg tacaaacggt
    15361 tcccatggat ttctgctgca aagggtgcat aggtccaaga cgattcatca ccagccgcca
    15421 cgacatccat atgcccagat aggcctaaca cttttcctga ttggcccttt tggtaacgag
    15481 caatcaaatt atctctacca tctctgtaag aaacaatctc tcctgttatg tcatgctttg
    15541 ccaaaagctt attcaaatag gctgctactt caccttcgtt tccattcact gatttaattc
    15601 ggatgatctc ttgtaaaatc gcgatttttt cttctttttt catctaatcc tctcccttgc
    15661 catttctttt tattgtattt gagtgtgctc gaattttcaa ataggagcaa ccaaagtaaa
    15721 aatgaagaaa gagagccact ttatgaaaaa tatgctgcta cttccctcct attttattta
    15781 tacgagagga cttgctcata gattcctatt ctactgttct atccttaaaa gctcttatat
    15841 gatgctagtt attgttagct ttcctttttc aaggatgatc ttttcttgta ccattctttg
    15901 taaacatcat atacagaagc tagttgcaaa aaaaactctc tacctgctga tatt
//
LOCUS       DAZULO010000066        15859 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000066 DAZULO010000000
VERSION     DAZULO010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.067"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..751
                     /locus_tag="ACG8OA_001988"
     CDS             68..751
                     /locus_tag="ACG8OA_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFS4976754.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="ACG8OA_001989"
     CDS             753..1589
                     /locus_tag="ACG8OA_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS4976755.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="ACG8OA_001990"
     CDS             1600..3354
                     /locus_tag="ACG8OA_001990"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4976756.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3630..3971)
                     /locus_tag="ACG8OA_001991"
     CDS             complement(3630..3971)
                     /locus_tag="ACG8OA_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS4976757.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4039..6210)
                     /locus_tag="ACG8OA_001992"
     CDS             complement(4039..6210)
                     /locus_tag="ACG8OA_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS4976758.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6421..7278
                     /locus_tag="ACG8OA_001993"
     CDS             6421..7278
                     /locus_tag="ACG8OA_001993"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS4976759.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(7349..8206)
                     /locus_tag="ACG8OA_001994"
     CDS             complement(7349..8206)
                     /locus_tag="ACG8OA_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4976760.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8191..10893)
                     /locus_tag="ACG8OA_001995"
     CDS             complement(8191..10893)
                     /locus_tag="ACG8OA_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFS4976761.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10906..12195)
                     /locus_tag="ACG8OA_001996"
     CDS             complement(10906..12195)
                     /locus_tag="ACG8OA_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFS4976762.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12345..13391
                     /locus_tag="ACG8OA_001997"
     CDS             12345..13391
                     /locus_tag="ACG8OA_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4976763.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            13393..15546
                     /locus_tag="ACG8OA_001998"
     CDS             13393..15546
                     /locus_tag="ACG8OA_001998"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFS4976764.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         4506 a         3308 c         2927 g         5118 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
     3421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     3481 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
     3541 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
     3601 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
     3661 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
     3721 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
     3781 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
     3841 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
     3901 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
     3961 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
     4021 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
     4081 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
     4141 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
     4201 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
     4261 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
     4321 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
     4381 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
     4441 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
     4501 attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc
     4561 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
     4621 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
     4681 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
     4741 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
     4801 atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat tcaaactttg
     4861 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
     4921 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
     4981 tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc
     5041 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
     5101 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
     5161 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
     5221 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
     5281 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
     5341 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
     5401 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
     5461 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
     5521 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
     5581 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
     5641 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
     5701 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
     5761 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
     5821 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
     5881 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
     5941 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
     6001 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
     6061 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
     6121 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
     6181 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
     6241 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
     6301 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
     6361 cttaaatatg ttttaaaaat ggtacaatta gtctgcaatt gcactgaaag gagtacactt
     6421 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
     6481 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
     6541 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
     6601 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
     6661 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
     6721 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
     6781 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
     6841 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
     6901 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
     6961 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
     7021 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc
     7081 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
     7141 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
     7201 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
     7261 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
     7321 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
     7381 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
     7441 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
     7501 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
     7561 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
     7621 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
     7681 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
     7741 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
     7801 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
     7861 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
     7921 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
     7981 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
     8041 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
     8101 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
     8161 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
     8221 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
     8281 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
     8341 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
     8401 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
     8461 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
     8521 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
     8581 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
     8641 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
     8701 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
     8761 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
     8821 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
     8881 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
     8941 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
     9001 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
     9061 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
     9121 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
     9181 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
     9241 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
     9301 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
     9361 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
     9421 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
     9481 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
     9541 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
     9601 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
     9661 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
     9721 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
     9781 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
     9841 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
     9901 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
     9961 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    10021 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    10081 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    10141 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    10201 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    10261 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    10321 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt tccaccacat
    10381 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    10441 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    10501 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    10561 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    10621 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    10681 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    10741 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    10801 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    10861 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    10921 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    10981 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    11041 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    11101 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    11161 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    11221 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    11281 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    11341 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    11401 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    11461 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    11521 ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    11581 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    11641 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    11701 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    11761 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    11821 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    11881 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    11941 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    12001 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    12061 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    12121 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    12181 atctttataa accactaata aaaccttctt tctatttgat atatctttaa agattatact
    12241 tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact ttttcatcca
    12301 ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgact caaccattgt
    12361 accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta cctgtagaca
    12421 gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga atcacttcca
    12481 aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc gggaaaggca
    12541 gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa acacgacgga
    12601 ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc aagggattga
    12661 accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac tttctacaaa
    12721 ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat gctcttgata
    12781 cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct gtcgtcatat
    12841 tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat ttccaaggcg
    12901 gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct tatttgtttg
    12961 gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt gaagcattgg
    13021 acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca ttagatgaat
    13081 tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac gatgttgtcg
    13141 ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa gatttttcaa
    13201 ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg acaaccatcg
    13261 cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact tggttagaga
    13321 caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga caatcaacaa
    13381 aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc gaatcagcca
    13441 aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat gaattatttt
    13501 gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga ccgcgttttt
    13561 caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag tcatatgctg
    13621 tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca aagttcttac
    13681 cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt acgttatggc
    13741 atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga ttacagcaaa
    13801 aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt tatagatcct
    13861 tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga tttttctttt
    13921 tattttatat tgagatttga atatccttta acaattgatg agttagctgt ttccaacaaa
    13981 aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat ccgatttggg
    14041 acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca aaaccaaagc
    14101 aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga tcgtatacag
    14161 ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata tcgggtgttt
    14221 ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca ctatgatacg
    14281 actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg ggatacgtat
    14341 aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga aatgctggaa
    14401 ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc tcctgatgaa
    14461 cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc tgtaaaagat
    14521 atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc tacggttcaa
    14581 agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata tggctatgtt
    14641 cctaatcact accacgaatc agtcaatcac acattagact atgcttatag cgatttttgt
    14701 attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata tcagcagcaa
    14761 tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc aaaagataaa
    14821 gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta tgcagaagga
    14881 agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat cggaaaatat
    14941 ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc cccaacgttt
    15001 gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc gatagaattt
    15061 ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt cagttatata
    15121 ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac cttcgacgat
    15181 tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg gtatatcttc
    15241 aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat cggtatgcca
    15301 ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat tgaagcaacg
    15361 cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacag agaatatacg
    15421 aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta ccaattaggg
    15481 atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt atcaaactat
    15541 aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa
    15601 aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa gcttttgaac
    15661 accgtttatt gagaggttgt gaaatcagcg ttctgacctg agtattagag agacaaatgg
    15721 caaaatagtt cctcatattt tgccattttt tgagctaata ccgagggtca gctgattgaa
    15781 caccgtttag tggagaggtc gtgaaaaagc tgtttagttt cgagtattaa gaaaaatttt
    15841 gtgaaaatgg ctttccaca
//
LOCUS       DAZULO010000067        15457 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000067 DAZULO010000000
VERSION     DAZULO010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.068"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            91..369
                     /locus_tag="ACG8OA_001999"
     CDS             91..369
                     /locus_tag="ACG8OA_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976765.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            388..1596
                     /locus_tag="ACG8OA_002000"
     CDS             388..1596
                     /locus_tag="ACG8OA_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4976766.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(1744..2109)
                     /locus_tag="ACG8OA_002001"
     CDS             complement(1744..2109)
                     /locus_tag="ACG8OA_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFS4976767.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(2256..3029)
                     /locus_tag="ACG8OA_002002"
     CDS             complement(2256..3029)
                     /locus_tag="ACG8OA_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4976768.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(3203..4063)
                     /locus_tag="ACG8OA_002003"
     CDS             complement(3203..4063)
                     /locus_tag="ACG8OA_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS4976769.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4063..5481)
                     /locus_tag="ACG8OA_002004"
     CDS             complement(4063..5481)
                     /locus_tag="ACG8OA_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS4976770.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(5538..5987)
                     /locus_tag="ACG8OA_002005"
     CDS             complement(5538..5987)
                     /locus_tag="ACG8OA_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4976771.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(6432..6695)
                     /locus_tag="ACG8OA_002006"
     CDS             complement(6432..6695)
                     /locus_tag="ACG8OA_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFS4976772.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(6794..8089)
                     /gene="rho"
                     /locus_tag="ACG8OA_002007"
     CDS             complement(6794..8089)
                     /gene="rho"
                     /locus_tag="ACG8OA_002007"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFS4976773.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8086..9357)
                     /locus_tag="ACG8OA_002008"
     CDS             complement(8086..9357)
                     /locus_tag="ACG8OA_002008"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS4976774.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(9522..10391)
                     /locus_tag="ACG8OA_002009"
     CDS             complement(9522..10391)
                     /locus_tag="ACG8OA_002009"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFS4976775.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            10494..10706
                     /locus_tag="ACG8OA_002010"
     CDS             10494..10706
                     /locus_tag="ACG8OA_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976776.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            complement(10683..12293)
                     /locus_tag="ACG8OA_002011"
     CDS             complement(10683..12293)
                     /locus_tag="ACG8OA_002011"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFS4976777.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(12515..12760)
                     /locus_tag="ACG8OA_002012"
     CDS             complement(12515..12760)
                     /locus_tag="ACG8OA_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976778.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            complement(13486..14826)
                     /gene="glnA"
                     /locus_tag="ACG8OA_002013"
     CDS             complement(13486..14826)
                     /gene="glnA"
                     /locus_tag="ACG8OA_002013"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFS4976779.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(14854..15246)
                     /locus_tag="ACG8OA_002014"
     CDS             complement(14854..15246)
                     /locus_tag="ACG8OA_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4976780.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4391 a         3289 c         2649 g         5128 t
ORIGIN      
        1 tacctttttc catcacttat gcgatttttt tccttttttt tattcgttat actgcagaag
       61 taaaattttt taagaataga gggaaaaaac atgaattact ggatgaacac aatcattaat
      121 cgattagaaa cagcatatca aacaagattt gatatgaaag cttctttagt ctttttaaat
      181 gatgcgtatc aaaactcgat cgaattgatc aaagcagtgg atgagaatcc aacaaacgaa
      241 tgcgaagaat ttttaaatct gtttatgtct acaagagatt tatttattcg ccaacttgtt
      301 gatcgctatc catccaatta tcacgatgta gaagttcaaa tacaaaaatt aaaagcatat
      361 tcagcttaag gggaatgtat aacatacatg gaaaagaaat tatttaataa atattttatt
      421 ggaatcacat tgatcaattt tattgtctac ctgatttatt atctgttgat ggtcatcatg
      481 gcagtcattg cgcaagacac cttacacgcc agcttgagcc aagcaggttt tgcttctggg
      541 atctatatca tcggaacatt gctggcacgc ttattcatgg ggaaacagtt agaactattt
      601 ggtcggaagt ttaccttaag aggcggcgcg atcttctatc tcctttcaac attagcatat
      661 ctttatacgc caactatcgg ggcattgtat ttagttcgat tcttgaatgg attcggctac
      721 ggtacagttt ctacagctac aaatgctatc gttactgcat atatccctgc atctaaaaaa
      781 ggggaaggca tcaattatta tggattaagt actagtttag ctgccgctgt tgggccattt
      841 ttaggaatga ttttgctgaa tatgactgat tttcgtttca ttatctggtt ttcaattgta
      901 ctggtcttct ttgttacgat tgcctgcctt gtctttcctg taaaaaatat tccattatct
      961 gaagaacaaa aaatatctct tcgctcatgg aatatcaata gttttattga aaagaaagca
     1021 cttttcatta caggaattgc ctttttgatg ggtctttctt attcaagtgt tttatcattt
     1081 ttatcttctt atacaaaagt aatccactta gttgctgctg ggtcattttt cttcgtcgta
     1141 tatgctttgg tcatcacatt tacacgtcca ttgactggaa ggattttcga tgtcaaagga
     1201 gaacaatacg tgatgtatcc tagctattta ttcttaacag caggattatt tttactgagt
     1261 gtaacgacga atagtgtgac attgcttatc tctggagcat tagtcggcct aggttatggt
     1321 acgtttatgt caaatggaca ggctgtttgt ttaaaaatcg tccgcgaaca tcgaatcagt
     1381 attgctttat ctacctactt tattggttta gacctaggct taggcgttgg tccttatttg
     1441 atggggatgc taaaatctct tacttcattt agaggattgt atgtgattgc cggagtcatt
     1501 ccattgatat gtacagcact ctatcttgtt aatggccgaa aacatagtca tgaagcagag
     1561 ggcgaattga ttgaatcatt gaaaaatgaa atttgatctt gatgaataga taaaaaaaac
     1621 cgttcagatc tgaaaataat ctgagcggtt ttttgagtaa aaatagttta tgattattct
     1681 taattaaaca tctatttatt tttaaagctt tcatttatcg ataagaatag tttaactgtt
     1741 gatttattcc attttccgtt catctggtac gatgccttga cttagcaacc acttgtggaa
     1801 gaaccattct gccacaccga tcaatagagc aggaatcaat actttcatcc aaatatcgac
     1861 tgtatcattc ataaaatata gatagagcca caagataact gcagctgaac cagcatctgc
     1921 gatcgtcgct gcgatgttcc ctactttacg taagatcaac agatccccta gcacatagat
     1981 catgactaca ataattaccc caacaatcgt tgaatccata aagctaacac cgtataaacc
     2041 tgtcagcacg acccacagaa tcgcccagat catgattgcc ttcactacca acgccttcac
     2101 atgtttcatt ttctgcactc ctttctcctg gataaaaaag taagattatc taacaagata
     2161 accttttcat tattatacca tcggctgaga gaagcgagca aagataaggt cctatctaaa
     2221 aatgaggaag ccacttagaa cattcacgta ataatttatc cgtacagaca ttcgatatgg
     2281 tgttccttta ctttcgcata ctgttctttt gtcagttctt tacttgtcac cagataatcg
     2341 atttcatgaa aagcaatggc ttgatgcatg aatttatgtg caattttttg ctgtgtaact
     2401 aaaacggcaa gccggtctga ttgttttcta aaagcttttt tgaccattgc ctctccttca
     2461 tgagtttctg ttactccttg gctgaaatct agcccgcgac aactcattat cgctaaatcc
     2521 gcataatatg acaatgcatc gttataggct tttgaaccgt tgattgttgc tcgtttcgga
     2581 acaattgccc ctcccaatac agatactttt gccgttgtat attcactcaa catagcagct
     2641 gtgacgatgc cattcgtaat gatctgcaaa ccatcaaatc ttgctaggtt tctgactaag
     2701 tgtaaagagg ttgtactgga atccaagaat aagaccatat catctcgaat caaagaagaa
     2761 gctgtatctg caatcttacg tttttctgtt tcttcttcta atttggtgat cttcatcagg
     2821 taaggcacgt caatttttcg attgtcaata agcatggcgc caccgcgaac tctacggatc
     2881 aatccacttt tttccagaaa aacaaggtca cgtttaatcg tggcttcgct ataatttact
     2941 aactcgatca aatcggtgac tcttgcaaat tggttgtttt ttagataatt cacaatctcc
     3001 tgctgtctat tctccacttc attcaacata aaatcacccc atttacttta tatagtaaca
     3061 ggaaaaaaga ggttgtgaca gaagtgattg gcttcgagaa ataagaagaa atttttcgaa
     3121 aaaactactt ctgaacatcg tgtgtggtta agagggtatg acaagactat tgtcataccc
     3181 tcttgaccac acatttttat ttttagtata aactagcctt gcctactgtg ttcaaaattt
     3241 ccattttatg tttgaccact tcttgtacct ttttattagc tgctggataa attgcattcg
     3301 gctcgtacat cgatggattt tcactcaata tttctctcaa agcattgaaa aagacgcttt
     3361 taagatctga gctcaaattc actttggcaa ctccgtattt tactgcttcg ctgacttctg
     3421 cgtccggatt acctgaaccg ccatgtaaaa caaatggaag atcgatttgt tggttcaatt
     3481 ctttcagtaa gtcgatattt aattttggag ttctgttttt cggatacaag ccatgcgctg
     3541 ttccaattgc tacagcaagt gtatcgattc ctgtttcttt cacaaaaatc ttcgcttgat
     3601 caggatctgt ataaatgatt gtttcagcac ctccttcagc agaaccatta ttcccaatcg
     3661 ttccaagctc agcctccact gagacattta ccgcatgagc taacgcaacc acttttttcg
     3721 taatctgtac attgtcgtcg aatgatttca aagacgcatc gatcatcacg gaagaatagc
     3781 cgtttcgaat cgcccgtaag acatcttcta tcgtaccgcc atgatccaag tggataacga
     3841 tcggcagttt acttgtataa gcataggctc ttactgccgc aacaaactca tcacttaaat
     3901 aggcgatttc atcaggatga atttctaaga ttactggtgc ttctaatctc tctgcttctt
     3961 cgatgattgc tttcatcata tcatagctgc aaatattgaa ggctggtact gcaaattgat
     4021 tctctttagc cactttcaat aactctttca ttgtatataa cattacgctt ccacctcatt
     4081 tgttagattt tcatttttcg tccacttgtc ttgttttttc tctttcactg tttttttcaa
     4141 tagtccaagc ataaaggctg tagtgactgt acctgatgca attgccaaga catacatagg
     4201 taaattgttc ataacatttg gaattagcat cacccaaatc cctccatgag gtgccattga
     4261 agtaactcct actgccatgg aaatccctgc agcgactgct gaacctgtca tcaaactagg
     4321 tatcacgcgg atcgggtcag ataccgcaaa cggtatagca ccttctgtaa tatacgaagc
     4381 gcctaaaatc cagcatgatt gacctgaagt ccgttcttct ttggtaaatt tatgtttgaa
     4441 taataaagtg gctaaagcaa tacctagggg aggtgtcatt cctgcagcca tacaagcagc
     4501 aatcggtgca tttactccag ctgacatcaa accgatcgag aaagtcgaag tcgatttatt
     4561 gatcggccca cccatatcag ctgccatcat gatcccgata agtaatccga acaatagccc
     4621 ggatgtattt cccaagctat tcagccaatt tgtcaaagca tccaacaccc atgtgatcgg
     4681 ttgttcaatc acaaatacca tcaaaagccc aataattaag atcgataaaa aggggacgac
     4741 aatcagctga taaatagcag aaaattcttt ttttacgcta attttatttg ccagaaaatc
     4801 acagatatac cctgctagca cgccaccaat cattccgcca agaaaacctg aaccacctgt
     4861 agaggccagt aatccggcaa caattcctgg ggcaaaacct gcttttccag caatcgatgt
     4921 agcaatcccc gcgcctaacg caggaaccat catggcaaac gctgtatctc ctccgatcct
     4981 tgaaaatgca gctgctaaca cattatagtc tgctgagttg ggatcagccg ctgtaatccc
     5041 aaaggcaaaa ctcagtgcga tgatgattcc gcctgcgatc acaaatggaa gcatgtaatt
     5101 cacaccattc atgaaatgat tatagattgt cggctttttc tcatttgcag ctggttcttt
     5161 ttcattagac acaacgaata cgccaatatc tctcaatgct tcgtctatta ctacttgcgg
     5221 ttctttgatt gctcggcttg tagagacacg ctttactttt ttcccggcaa atcggtccaa
     5281 ttcgatttct ttatctactg caagaatgat tgcatctgcc tctttgattt cttgttcaga
     5341 taactgattt tctgttttta caccttgtgt ttcgaatttt gcttgatggt ttagatttct
     5401 tgctgttact tctaattttt ctgccgacat ataagtatga gcaattccag tcgggcaagc
     5461 agttacacca acgattttca tgattatttt cctccttttt cttcactatc gatactcgga
     5521 caaagactct actattttca ttcttgacta atctgtctga tgatatctgc tggcgtagtg
     5581 gcttctttta ttccctttct aaaatctgca ttcatcaatc gtctggagag tgtgctgaga
     5641 atctctagat gcatatctgc tgatttttca ggaactacga tggcaaaaat atattcaatt
     5701 ggcatttcat ctatcccgct atagtccgtt aatttggctg tatgaaaaaa acacagtgct
     5761 ggttttgtca catatttgct ttttgcatgg ggaatcccaa atccatcttc tattcctgtg
     5821 gagatctcct cctctcgttt gaatagtgcc ttaacgactt tttgaacatt tcttacctta
     5881 tttgtttttt tcaacacatc ccctattcgc tcaaataatg atccttttga atctatcgat
     5941 ggatcgaaaa caatcagttc ttctgcgatc aattgatcaa acctcataaa aaacctctcc
     6001 cccacaaaaa atgttaacgt tttcttggaa ctgaggtcag tatagcatcg ctagctgtac
     6061 cgttcaatat tatgaatgaa cagaggaaaa agacccatca tccgcgtgta taagtcatta
     6121 aaatgaacga tctagctcac aaacaatcgc aagtctataa caaaacaaaa agccttcctt
     6181 gaaaggaaag cttagcttct aattttaggt aaatggtttt ttacttcgaa ataacccctc
     6241 ttcatttcgt taatataagg tgtttttatc aagtggataa ttaaaactat atactagttt
     6301 ttcattgttt tgtgcattat gatcctacga ctttttcagc atcaaacgaa gttttaaatt
     6361 ggcaaaaaac ataaaaaaac agactatccc tatcagatag tctgctaaac gttgagttat
     6421 cagcgttttt attattccgc agcgtttgcg tctttgagac cgtatttttt gttgaaacgg
     6481 tccacacgtc cgtctgcttg tgtgaatttt tgacgtccag tgtagaatgg atgtgagtca
     6541 gaagttactt cgacacggat gactgggtat gtattaccgt cttcccattc aactgtttct
     6601 tgtgaagttt ttgttgaacc tgacaagaat ttgaaaccag ttgttgaatc catgaatact
     6661 actgggtggt aatctggatg gatgttttct ttcatgattt tttcgctcct ttgccctgct
     6721 ccatttgctg aaccagagtt gatttgtcga tttacaacat taacatctta acatgtttca
     6781 gtcaaggttg caattatctt ttcagatttc tggtcggatt ttttttacca aaggagacat
     6841 cttgaaattc tttgaaaaat tgctgattgt tttttgtttt acgcaaaaag cggatcagtt
     6901 gttccgtata ttctaatgaa tctccagtca tattttttcg aagtttccaa atttcttcta
     6961 attcttcact tggcatcaat aattcttctt ttcgggtacc tgattttttg atatcgatgg
     7021 ctgggaagat ccgacgttca gataattcac gtgaaagttg taattctaga tttccagttc
     7081 ctttgaattc ttcatagatc acgtcatcca tacggcttcc tgtatcgact aaagcggtcg
     7141 ctaaaatcgt caaactgcca ccttcttcga tgtttcttgc tgccccgaaa aatttcttag
     7201 gtttatacaa tgcggctggg tcaatcccgc cactcaatgt acgtccgctt ggtggaacga
     7261 ctaagttata ggcccgagca agtctagtaa tactatccat caagataacg acatctcgtt
     7321 tatcttcgac taaacgcatt gcgcgttcca atactaattc agaaactcga gtatggtttt
     7381 gcggctgcag atcaaatgta gaagatacca cgtcgccttt cacgcttcgt tctagatccg
     7441 ttacttcttc cggtcgttca tcaatcagta gaacgattaa ttctacttcc ggatagtttt
     7501 cggtgatacc atttgcgatt tcttttaata cagaagtttt ccctgctttt ggcggtgcga
     7561 cgatcaatcc acgctgacca aaaccgattg gagaaaagat atcgatcatt cgggtagata
     7621 gtctttgcgg tgttgtttcc agttgaagct gtctttgcgg ataaagaggt gttaaggctg
     7681 gaaaatgcgg acgttctttt gcttcttccg gatcttttcc attaacgctt tccacgtgca
     7741 tcaaaccata ataacgttcc gattctttag gtggacgggc ttttcctgcc actttgtccc
     7801 catttctcaa actgaatcgt ctgatttgtg aggcagaaat atagatatct tctgcacttg
     7861 gtccataatt gattggccgt aaaaaaccgt aaccatcttg accaacgata tccaatattc
     7921 cttccatata ataaaatcct tgtttctctg cttgtgcacg aatcacagct agagataatt
     7981 cctttttatt catcttactg taataaggaa ttttaaattc tttggcataa ctataaactt
     8041 cttttaacgt tttgttctct agttctgcca ttgtcaaata atcgctcatt cagctcccac
     8101 ttcctctatc atttcaattg atgcccctaa ggctgttagt ttctcgataa tatgatcata
     8161 accgcgtaag atataatcta cattgtaaat cgtcgttgtc ccttctgcca atagtccagc
     8221 aatcactaaa caagcacctg cccgaagatc agaagccacg acttctgtgc cgtgaagttt
     8281 atttggacca ttcacgatga tcatatttcc ttccacggta atatcagcgc ccattcgtgc
     8341 tagttccggc acatggttca cacgtttcgc atagatcgta tcgatgattt ctgaagtgcc
     8401 tgtagtcatc aatagtaacg cagtaagtgg ctgctgcaga tctgtggcaa atcctggata
     8461 aggataggtc atgatgtttg cgggttttaa ttctttcgag gggtaaactt cgatttcatc
     8521 ttcgctgatt ttcatatgga caccaatttc ctgcaatttt gctataaagc tttccaaatg
     8581 ttcaaagatc acatttttca ctttgatacc atttcctact gcagctgcta atgctagata
     8641 ggttcctgct tcgatgcggt caggaatcat gaaatgccga cagccatgta atgtttcgac
     8701 tccttcgata cggatcacat ctgtacccgc tcctcgaact tttgccccca tattattcag
     8761 taaagttgcc acatctatga tttccggttc tcgtgcagcg ttttcaatta ctgtctgtcc
     8821 ttttgctttt acagcagcta acatcacatt gatcgttgct ccgactgaaa ccacgtccat
     8881 gaaaatccga ttgccacgca atcctttatt ttctgtccgt aaatacatgg caccatgttc
     8941 atttgtcact tcagcaccta gagcttcaaa tcctttgacg tgtaggtcaa tcggtctagg
     9001 gcctaaataa cagccacctg gcaggcctac taccgcttcg ccaaatttac caagcagtgt
     9061 tcccataaaa taataagaag cacgcaaact attgattttt ccgtgcggca tcggaatcga
     9121 tacgacttct cgaggatcga tttctagtat gttgttggaa aaatggacag ttgcgcccat
     9181 gatctctaaa atttcaatta atgaatgaac atcttgtatg tccggcacac cttccaagat
     9241 cactggggaa tcagctaaga ttgcagctgg aatcaatgcg actgcactat tttttgcacc
     9301 gctgatcgtt acttcaccag ctaaaggtcg gtttcctttg attacgattt ttttcatctg
     9361 tttccacctt gtttattaat cttttttcat ggatatgctt gtattttatc atagataaaa
     9421 aaaagaaacg agcatggtct gccgttttaa gtaaaaaaga atggagagcg acttcaaaag
     9481 tcgttctcca ttcctatata tcgtatctgt tatgtggatg cttacgcttt gttagctgaa
     9541 ccgaaccagt cgatatgaga tttagcatct tcaacgattg ctgctttacc tggagctaat
     9601 aatttacgag ggtcgaatcc tttaccttct ttatctttac cagcttcgat atatttacga
     9661 gtagctgcag caaatgataa ttggaattca gtgttaacat ttacttttga gatacccatt
     9721 gagatagctt tttcgatttg atcttgagga atacctgatc cgccatgtaa tactaaaggc
     9781 atgtctggac ctacagcatc agcaattgct tgtaagtggt caaaagctaa tcctttccag
     9841 ttttctggat attgaccatg gatgttaccg ataccgcaag ctaagtagtc gatacctgta
     9901 gcaaccattt gtttgcattc ttcaatatca gctaattcgc cgttaccgat gattccgtct
     9961 tcttctccgc cgattgaacc aacttcacat tcaactgaga taccttttgc atgtgctttt
    10021 tcaacaacgt ctttagctaa tttcaagttt tcttcaaatg gtagatgtga accatcgaac
    10081 attactgaag tatagcctaa ttcgatacat tccaaagcag cttcgtaatc accatggtct
    10141 aagtggatag caactggaac agtgatgttc attgcttcaa ctaaatcagt gattaaatct
    10201 ttacagattt tgtaaccacc catgtattta gctgcaccca ttgatgtttg gataagtact
    10261 ggtgcttgtt ttgcttcagc cgcttcaagg attgcttgag tccattccaa gttgtttgtg
    10321 ttgaatccgc cgacagcgta accgccttta cgagcagctt ttaaaaattc agctcctgat
    10381 actactggca taataaaatt cctcctaagt tttgtaaaac taaatttgtt aagacaattc
    10441 ttaaccaacg accctatttt accaaacatt tcggtagttt tctactaaaa tcaatgaaaa
    10501 tgaaaatttt caagaagttt tttgcaaaaa ataaaatatc caatagcgct ttaatcgaaa
    10561 aaagtctttt gctttctatc ttttttccta caaataataa caaatcagaa aaagagaaaa
    10621 gtgacaagaa aattgcttat ccccttctct tgctactttt ctctttctat caagttcaaa
    10681 aattattttg tttcgtgatc tgctaatgat gctccgacaa atgctttgat taatttttgc
    10741 gggcgatttg gacgagaaat caattctggg tggaattggc atcccacaaa gaattttttc
    10801 tctggcagct ctacgatttc tactaaacgg ttatctggtg agacacctga aaagactagc
    10861 ccttgttctt caaacaattg acgatatttg ttattgaact catagcggtg acggtgacgt
    10921 tcttgcacaa catcggctcc atcatatgct tcagccgtct tcgttccttt tttgatttta
    10981 catgggtata atccaaggcg taaagttcca cccaaattct cgatgttttc ctgatcagcc
    11041 attaaatcaa taatattgtt tgctgtatct ggattcgttt cagctgatga cgcatcttct
    11101 aatccaacga cgttacgtgc aaattcgaca caagccatct gcataccaag acaaatacct
    11161 aaaaacggaa cgtcattttc gcgagcataa cgaatcgctt cgattttccc ttctactccg
    11221 cgatcaccaa atccaccagg aaccaagatt ccatctgcgt ctttcaaacg ttcttctaca
    11281 ttttctctcg ttaattcttg tgctttgatc caatcgatct cgatgtctga attataggcg
    11341 aaaccagaat gtttcaatgc ttcaactact gagatatatg catctggtaa ctcgacatat
    11401 ttaccgacaa gcgcaatccg tacttttttc ttcaagttca agacacgttc ttctaattct
    11461 ttccattctg tcatgtctgc ttctggtgta tctagtttta aatggtcgca aacaatttga
    11521 tccattcctt gtgcttgtaa aactaatggg atggagtaaa gtgtatctac atctggcgac
    11581 tcgataactg cttctggttc gacatcacaa aattgtgcca atttgttttt cacgctttga
    11641 gaaacaggtt gctctgtacg taccaccaaa atattcggtt ggatacctaa accacgtagc
    11701 tcttttacac tgtgctgcgt tggtttagtt ttcatttctc cagcagcttt caagtaagga
    11761 atcaatgttg tatggatata cataacatta tctgtaccga catctgcttt catttgacga
    11821 agtgcttcaa ggaatggcaa agattctata tcccctactg ttccccctac ttctgtaatg
    11881 atcacatctg aatcagtcat tttagctgct cgcatgattt tttctttgat ctcatttgta
    11941 atatgaggaa taacttgaac agttgctccc aagtattcgc ccttacgttc tttacgcaaa
    12001 acttcagaat agatttttcc tgtcgtcacg ttggagtatt tgttcaaatt gatgtcaata
    12061 aaacgttcgt agtggcccag atccaaatct gtttcagcac catcatctgt tacgaatact
    12121 tctccgtgct gatatgggct catcgtcccc ggatccacgt tgatgtatgg atcaaatttt
    12181 tggatagtta cttttaagcc gcggtttttc aataagcgtc ctaaagaagc tgcaacaatc
    12241 ccttttccga ttgatgaaac gacaccgccg gtcacaaaaa tatattttgt cataagttca
    12301 aaaactcctt tttaatttat gtgcaggaaa agtctcagac aataaacaaa gctccctatt
    12361 cttcaataag aatagggagc cgaaatttct gtaaacttct gaccctctaa aagggtgccc
    12421 aaggtgtata atacaagctg actaagggaa agtcaagtac tcaaatgaat actatccgct
    12481 tacaattttt tctttttcga tatcctttga gaaattattt cttaagtcct ttatgagtgc
    12541 aaaaaaaatt taacccacat aataagcttg atataaacat agctaacgtt aaacctaagc
    12601 aaatcagaaa atcgttactg aaaaatttta agtgaaataa tagataaaaa aataaaacaa
    12661 acattatgat agataatatc ccacctaaca aagaactact ttttcctact tgcagcaaca
    12721 gtttatatag cttatcgtag aaacaatctt tttctttcac ccttatttac ctcctctacg
    12781 taaaaaacgt agtccacagt tctcggattt ttggcatatc ttttctatta aataatatca
    12841 aaaacaaaac aattccacta attttttcaa agatgacctt tttttcaaca aaagtgcctt
    12901 gtagggattc attgaaggta acgaatcaca taagtaaaaa ataaggagat gcgacaaaag
    12961 tcgtgcctcc ttatttctaa ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
    13021 caatatccat caaacatgag aagctgtttt tgaaattatt tcttgtgatt cggagttaaa
    13081 tggtaggctc acaacctctg gataaacggt attcaaaagc cagtttctcg gtattaagcc
    13141 aaatttatgc aaaaatgtag aaagccattt tcacaaaatt tttcttaata ctcgaaacta
    13201 aacgtctttt tcaaaacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa
    13261 gttcagccaa ttcaaaaaca agaaaagctg tttttgcttg gctattctta ttacttgttg
    13321 cagggcagct ttttcacaac ctctccacta aacggtgttc aatcagctga ccctcggtat
    13381 tagctcaaaa aacggcaaaa tatgaggaac tatttttccg ttagtttctc taatactcag
    13441 gtcaaaacgc tgatttcaca gcctctaatt cacggtattc ctgttttaat aaagttctag
    13501 atattgttct ctttcccact cggaaacagt ttgtctgaat gccgcccatt cgagacgttt
    13561 tgcttcaaca aaatttgaga agatatgttg tcctaatgcg tcaatcatta cattatcctt
    13621 acgcaattct ttgatagcat tatggatagt tgaaggtaag tcctggatat gtgcagcttc
    13681 tctttcttcc tcatccatta cataaatgtt acggtcaaca gctgctggcg gtgtgatctg
    13741 attacggata ccgtctagtc ctgcttgcaa caatactgcc attgccaagt atgggtttgc
    13801 agatggatca actgaccgca attccaaacg tgtggataaa ccgcgagatt ctggtacacg
    13861 tactaatggc gaacgattac ggccactcca tgcgacataa acaggcgctt cataacctgg
    13921 aacgagacgt ttataagaat tgactgttgg attgcaaacg gctgtgtagg cacgagcatg
    13981 tttcagcaaa ccacctaaga aatgataagc tgtttcactt agttccaatt caccgtcttt
    14041 atcatagaaa acatttcctt catcattgaa caaagacata ttgcaatgca taccagaacc
    14101 attgatccca aataacggtt tcggcatgaa agtcgcgtga agtccatgct tgcgtgcaat
    14161 cgttttgaca acgagcttga aggtttggat attatcacaa gcatctacca catctgcata
    14221 tttaaagtca atttcatgct gtcctggcgc tacttcatga tgtgaagctt ccacttcaaa
    14281 gcctagactt tctaattcca acacgatgtc acggcggcaa ttttcaccta aatctgttgg
    14341 tgcaaaatca aaataaccac ctttatcatt caaggtcgtt gtgatcccac cgtcttcgtc
    14401 taatttaaat aagaagaatt ctggttcagg tcctaaattg aaagaggtaa aaccaaggtc
    14461 gcgcatatct ttcaatgctc gtttcaaatt cccgcgagga tcgccggcaa atggtgtccc
    14521 atccggatta tagatatcac aaattaaacg agcaactttt ccatgagcac tttcccacgg
    14581 gaagatcatc caagtggaga gatctggata caaatacata tcgctttctt caattcgaac
    14641 aaatccttca atcgaagaac cgtcaaacat cattttattt tctaatactt tgtcgatctg
    14701 gctcactgga acttctacat ttttgatcgt acctaagatg tctgtgaaca tcaatcgtaa
    14761 aaatcgtacg ttttcttcct caatgattcg tttgatttct tcacctgtta tctgtttctt
    14821 taccatggta tccctcttcc ttttttaatt tattcagtca aagaattgtc tttgcaatcc
    14881 ttgcgactga taatgacctg ttttgcttaa cccaccttgc gccaaaatct catcacgaaa
    14941 aatttgtcga acgtcatgat ctgtcaacgg cttcttgttt tttgcttctt gttttttcgc
    15001 ctgtgccatt tcgtatacac gtttgatccc agccatattc aatccctctg aaagatagtc
    15061 tttaatctca agcaacatat caatatcatt cagcgaatac atacgtcggt ttccttcgct
    15121 tcgttccgga tgaactaaat cttgttcttc ataatagcga atttgccgag cagacaaatc
    15181 agtcagcttc ataactgtac caattggaaa tacggacata gatcggcgca attccttttc
    15241 tcccatcaga cgtccccttt cgttttataa gttggctaaa gtgtaccaat taaaaaaaat
    15301 attgtcaact gcatatgtta ggttttctaa catgagatct ctatttttta taaaataaca
    15361 aaaaaataag aacacaaaaa aggtatctag ttgattttcg taccagatac cttttatttt
    15421 ttattatact gtactatcct ttattagtgt ttttatt
//
LOCUS       DAZULO010000068        13690 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000068 DAZULO010000000
VERSION     DAZULO010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.069"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            139..477
                     /locus_tag="ACG8OA_002015"
     CDS             139..477
                     /locus_tag="ACG8OA_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976781.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(682..1257)
                     /locus_tag="ACG8OA_002016"
     CDS             complement(682..1257)
                     /locus_tag="ACG8OA_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HFS4976782.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1989..2570)
                     /locus_tag="ACG8OA_002017"
     CDS             complement(1989..2570)
                     /locus_tag="ACG8OA_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4976783.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2753..3379)
                     /locus_tag="ACG8OA_002018"
     CDS             complement(2753..3379)
                     /locus_tag="ACG8OA_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS4976784.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3401..4732)
                     /locus_tag="ACG8OA_002019"
     CDS             complement(3401..4732)
                     /locus_tag="ACG8OA_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFS4976785.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4887..5288)
                     /locus_tag="ACG8OA_002020"
     CDS             complement(4887..5288)
                     /locus_tag="ACG8OA_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS4976786.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5374..5580)
                     /locus_tag="ACG8OA_002021"
     CDS             complement(5374..5580)
                     /locus_tag="ACG8OA_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976787.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5675..7177)
                     /locus_tag="ACG8OA_002022"
     CDS             complement(5675..7177)
                     /locus_tag="ACG8OA_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFS4976788.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7195..7650)
                     /gene="argR"
                     /locus_tag="ACG8OA_002023"
     CDS             complement(7195..7650)
                     /gene="argR"
                     /locus_tag="ACG8OA_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS4976789.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7748..8365)
                     /locus_tag="ACG8OA_002024"
     CDS             complement(7748..8365)
                     /locus_tag="ACG8OA_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS4976790.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8586..9959
                     /locus_tag="ACG8OA_002025"
     CDS             8586..9959
                     /locus_tag="ACG8OA_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4976791.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10034..11146
                     /locus_tag="ACG8OA_002026"
     CDS             10034..11146
                     /locus_tag="ACG8OA_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4976792.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11218..12855)
                     /locus_tag="ACG8OA_002027"
     CDS             complement(11218..12855)
                     /locus_tag="ACG8OA_002027"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFS4976793.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13023..13631)
                     /locus_tag="ACG8OA_002028"
     CDS             complement(13023..13631)
                     /locus_tag="ACG8OA_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4976794.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3690 a         2885 c         2452 g         4663 t
ORIGIN      
        1 actaaaaaaa taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat
       61 agctaacccc ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag
      121 acaacaggcc atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct
      181 gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg
      241 atcaaagaat gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac
      301 tttgctattg tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat
      361 ccaactcata tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca
      421 actgccaacc agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga
      481 taaacatcgt taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc
      541 tatacattag aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt
      601 tcttctgttc tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct
      661 ttctttgaaa aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt
      721 aatgattttt cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa
      781 gacaagcggc attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat
      841 gattgattct gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat
      901 tttttcctta tttgaaaaca taaatttgtc tttttcttca ttccactcat aaaagccact
      961 cattgggaaa acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac
     1021 tgtctctgca cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt
     1081 catgatttcc ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga
     1141 cggaaaaact tctccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc
     1201 tcgataataa ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct
     1261 aatccctccc aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac
     1321 gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg
     1381 tattctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct
     1441 ttttgaaatt gttccttgtg actcggagtg aaacggcagg cttacgacct ctgggtaaac
     1501 ggtgttcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcca
     1561 ttttcacaaa atttttctta atactcgaaa ctaagcgact ttttcacaac ctcttcctta
     1621 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagca
     1681 gctattttta tttggctgtt cttattactt gatgcagggc agctttttca caacctctcc
     1741 actaaacggt gttcaatcag ctaaccttcg gtattagctc aaaaaacggc aaaatatgag
     1801 gaactatttt gccgtttgtt tctctaatac tcaggtcaaa gcgctgattt cacaacctct
     1861 aatacacggt gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaattt
     1921 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
     1981 tctaattatt aaagaacctt ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc
     2041 tgcctaagta gtgatagatc ctgtgtgact ttcaacaata agatacttcc ctggatttca
     2101 gcaatcaacg ctttggcttg acggtctgct tcttcatcgg acagtttttc ttcagaaagt
     2161 gccaggatat tttgtttcgt atacttgacc cattgattga ctaattctag tagcaacaga
     2221 cggtattctt catccactgt actcaactca ataatcaaat tacctacagg acagccatga
     2281 taagtgatct gtgtctcatg atcagcgaaa atccagtcca acatttcttc taatgcttct
     2341 tctggctgtt ttccagcatt taagatgtct tcgaacaatt ctgtctgcca tacgtccata
     2401 tgcgcatgga tcaccgctag acaaacttct ttttttgaag aaaagtaata ataaaattga
     2461 ccttttcctg tatcagaagc tgctagaata tctcggattg acgtttcatg atagcctttt
     2521 ttataaataa gatctttggt cgcttccaag atcttttcct ttgttttcaa tgatattctc
     2581 ctttttcatt tctcatagtc ctatattata cagtaaattc atcatttttt cttcttaatc
     2641 gtttaaaaaa gactgtgcca aattatctga cacagccctc actataaaac tgttctcata
     2701 tagctagatg ttcattgatt caagaactgc gaggacaaaa gcaattccta tcttatgata
     2761 gttgctctaa tacttcctct gttgtcatca cttgatctgc aataaagcca aagttcgtca
     2821 acgctgcatc cattgcttct tggccaggag caccaattgc atctgtcgct accacaacat
     2881 tgaatccact ctctaccaat tcacgcatat gggaatcgct acacaagttt ccgttcattc
     2941 caccaagaat tactgtgtcg attccatttt ttctcaattg gagcgccaaa tcattgcttt
     3001 ctggtccaaa gattttatga ggagagctga tgatcgtatt ttcatccaaa taaggtttca
     3061 attcttcgat aaaatcggct cctgaacctt cttccactga ttcatattga ctttttctag
     3121 caaacatctg ctgctcaagc atcatatgtt ctcctgcacc gccaaattgc catttatggt
     3181 catgtgggta aaaataatga ggagaaataa agagtttgta tcctttttct tttgcttgtt
     3241 ttaccatttc aatcatattt tcgatcgtat tgtatttttc taagatatct tttgtcaaac
     3301 caaagccacc gcctgctggt ttcaagaact cgacttgcgg gtcagtgaga actactgctg
     3361 tatttttatt attgaacatg gtggattctc cattcgttat ttattttaat acatcattta
     3421 gagcttcact cattgtcgga tgagaataga tttgatctct caataacgta taaggaagtt
     3481 ttccattcat agctagcgaa atcaagttga tgacttcatg agaatcttcc gcatagatac
     3541 ttgcccctag aatcaagtca gtttcagggt cgactaaaac tttgaataca cctttaggat
     3601 cttctaatac ttgtgcttta gggacaccgc ttgtcatata cttgaataat ttgtaattga
     3661 cgccttgtgc tttggcttgt ttttctgtta aacctacatt ggataaagtc ggagtgataa
     3721 atacactgta aggcacagct ttccggttat tcgttgttcg tttattctcg cccttcaact
     3781 gatccataat gattcgataa tcatctagag agatataagt gaactgcaat ccgcctttga
     3841 cgtctccaat cgcccaaatg ttgtctgcat ctgtacgaag gtattcatcc acagcgattg
     3901 ctccgcgatc tgttagttta acatctgtat tttctaatcc aagtgatgct gtacttggtt
     3961 ttcttccagt tgcagccaaa acttttgacg cttggattgt tacttcttgg ccatcttctg
     4021 taaaggtgac ggcagctgtt gattcttggt ctgctacttg atctacagat acacctagat
     4081 ggaattcgat tcccgcattt gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt
     4141 cacgtggaag aaattcagaa tgggcatcaa gaacgacaac tttagatccg tattcattga
     4201 acattgatgc aaattccaag ccaatatatc cagccccgat gatcactaat ttttccggta
     4261 attgttcctg atccatcgct tgcgtagaat caatcaagta gcgacttgta ttcaatcctt
     4321 tgattggtaa aatgattggt tcagaaccag tattgatgaa aatccgttcg ccttcaacaa
     4381 tcactttttc accattattc gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac
     4441 cgtccatcac tgtcccagtt gcctcatcgc tgatcatatg ataatttttg gcgcgaagca
     4501 tgcccgtcaa tttttctttc cgagcaacag cctctgtaaa gtcgattcct ctttctccat
     4561 tgaaaatcaa tgatttagaa ggaatacagc cgatgttgat acatgttccg ccatacatac
     4621 gtgttgactt ttcaacgact aaaaccgatt caccttttga agctaacgcc ttcgctaaag
     4681 tcttaccgcc ctttccaaaa ccgatcacaa tattttgata tttttccatc atttttccct
     4741 cgcttctttt ttacctaccg attggtacaa aaatatgata accgataatt gtctattctc
     4801 caaatcatct gctttatttg tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt
     4861 agtctggttc gatttccttt attcttctaa acaatcgtta cttctacatc gatatttcct
     4921 ctagttgctt tggagtaagg gcagaattca tgcgcatctt ctgcaatttt ttgggcttct
     4981 tcttcgctga tgccatcgat atgcacttct aatactacac cgatctgata gccgccatca
     5041 tctaaattat aaagagagac acgtgctttt actgtggact ctgctgtcat tcctgcttct
     5101 ttgatcatat gttgtaccgc cccattgaaa caagaagcat aacctgctgc aaataattgt
     5161 tctggatttg ttccttctgc atggattcct ggaggagtga ttttcatatg catcgagcca
     5221 tttggtgcct cgacttctcc attgcgcccg ccatggttga tcattgctgt ttcgtatgct
     5281 tttttcattg tactgctcct ttcgattgac catttgctac ttggctataa ttatagtgta
     5341 tgacaagtaa ggcgctttct acaaaaattc tgcttattcg aataccagaa tgccgtcttc
     5401 tactattttt ccaaaaaaca aaaaaatact actcgtgaac aacagcgtca tatttgttcc
     5461 aaaataaata gcaatctttt ctacataatc ttttatatga ttgattttaa gatacatata
     5521 taccagaaag ttgaccccat aaaccaatac tgttaaaaaa ataaccatat tcgttaccat
     5581 tttttcctcc ttttgctggt ctccctacca gcactcgatt taaccatacc actttttttg
     5641 ccgtccaagc aactagcctt tcaaaaagac tggtttattc tgctgttaat gattcaagat
     5701 cagaaaaatg tccgaataat tttgctaatt ttactaacgc caacttgttc tttccgaatt
     5761 catcattttc tatctgtaat actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc
     5821 agccgtttcc gtatggctct tctagatcat atcgaatacc ttcttcattt tccgggtcga
     5881 agttcatccc tttttcttct tccaacagtc gcttcatttc ttctaatatc ttgtttcctt
     5941 gttcgcgtgg ttcatcgctg aacaatttta atcggatttc ttgtacttct acgggctgtt
     6001 tcaactcttt gatttgttca tctaatgtga taccttttct ttttaattct ggcaaaagca
     6061 tcaggatctt tgcgatgaca tatgcaccat catccagaaa atagttttcc ttgaatgcag
     6121 cgtgtccgct tgtttcgatg gctagctgtg tatctttccc aagacggtta agttctaatg
     6181 ctttattgat tacatttcga tagccggaaa tataacgaac ttggatgcct cctttttctt
     6241 caatgaatct ttgtagatga gaagaagtag gggaattcgt cacgattctt gttcctggat
     6301 gctcttttaa tacgattgta cttaatactg cgatcaaatt atttcgattg atcactgttc
     6361 cagaagcact gacaactgca gaacgatcca catctgtatc gaaaatgatc ccaagatctg
     6421 cttcatttgc taaaacagcc tgcttgatac tttccatcgc ttctttatta tcaggatttg
     6481 ggatatgatt aggaaatcgg ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac
     6541 ctaagctatt caatactttt tcagcaaaga atccaccagc accatttccg gcatcaacaa
     6601 tgattttcca accatttagt ggtttgcttt ctagtgttcc aatccccttt ttaatttttg
     6661 tgactaaatc ttgcgcataa ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt
     6721 cgctttttat tttttgctgc tgtgatagga taaaagcaat atcttctttt tctgctcctc
     6781 cttctgaggt aaagaatttt attccattat aataataggg aagatggcta gcagtcagca
     6841 taaccgcagc atcgcacgag aactgttcaa attgagtgga cataaacata gcaggcgtag
     6901 tagctaattc gaaatcgatg acatgtatcc cttgttcttt cagactttgg atcaacgcct
     6961 ctttcagtgc tggtccgctt atcctactgt catgaccaat cccaatcgtc cattttttat
     7021 ttggatcggt tgatttcttc tttttcttta tccagtttgc tagtccccag ccgatctttt
     7081 ttaccatgtc tgtcgtcaag tttacttcat atccttcaac tggtaatgcg attcctcgaa
     7141 tatccgatcc attttgcagc gctttcatat ctttcatata aaagtcctcc tcatttattc
     7201 cctcattttt tccagctttt ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat
     7261 aatgaatgta tctacaccgg ccatcgttcc gacaacttca gggtaagcca cttcatctag
     7321 ccaattcgtt accacatcgg caccatttcg ttcagtcaat acgataacca tgaactgaac
     7381 agactggata cgcaaaacct cgcgtctaat tgctgtacgc aaacgttctt catttaaaga
     7441 aacagatggc tggttaaata atgtatagcg tactattttg ttttcatttt gactttttac
     7501 taacttcatt tctcgaatgt ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc
     7561 atgcagccgc gcaagcagct cttcctgtgt cccaataggt tgttctgtaa tgatctggcg
     7621 gatcagtgct tgtcgctgtt tctttttcat attagacacc tctcaatgtt gattatagca
     7681 aaagataagt aatatttctt catattttgg tattttttac ttttattttt tgttttcatt
     7741 caggttgcta acctttttct attttaacac gcaaaatcgt cttcattttt tcaggtgctg
     7801 attttcttgg atcgctgatg taaatctctt tgtgttcttt agaagctctt tcatagcctt
     7861 cttttgcgat gaatgcttct aatttctcaa acgttgctgg ttcttcggca tagggcccga
     7921 cgtgtaagat ttgggcacaa agaccttctt ccatcttttt gaatactagt tgttctttca
     7981 agtcttccgg aagtttgcta gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa
     8041 agtcaggctg tcggatcatc aattgataag aaaaatgttc ttttttcatc actggttcat
     8101 gcaaatactt ttcttgcagt ccccattgtc cttccagtgg atagacagta tacatctgat
     8161 aatcaggaat cagccaattt tttttaggcg acatgcgtat tgcatagctg actgcatata
     8221 gacaggctac tcttcgttta aagtcttcct gattcgggtc gccgatccct ctgatgctaa
     8281 agaatggttt tttaggaacc tctagtattt gcgggctttt tttcatacta tagctcttgt
     8341 ctttacgcca ttcaattttt tccatttttc ttcctccgcg aaattccatt tagttattta
     8401 ttatcatacc tctttttgag tgacttggct aatcgtgttg acactatttt tcagtttgtg
     8461 ctagaataag tgaagtaaat aaatcgtaca gataacacgt ttcggcgtgt cgagagagag
     8521 gcggtttcta tttttataat agaaatcact ctcttttttt acgaaatttt ttagaggtga
     8581 tgtaaatgac aaaacaaaaa cagctgcttt tagtacctgc agtaattgca acagggatga
     8641 tgtcttttgc gggcgtattg atcgagacgg ccatgaatgt aactttccca actcttatca
     8701 gacagtttga tttaacgact gcacaagtcc aatgggtaac aacgatttat cttttgatga
     8761 tttcgatcat tgttccgctg tctacttatt tgaatcgcaa ttttagtctc aaaaaactct
     8821 ttttagcttc aaatctgctt ttcttggcag gtataatgat cgatttcttc tctcctacct
     8881 tcatggtcct attgtttggt cgattgctcc aaggtgcagc gacaggtatc gctcttccgt
     8941 tgatgttcca tattatcttg acatttgccc ccctcaatcg acgaggcgcg atgatgggtg
     9001 tcggtacttt gacaacggcc attgctccag caattggccc cacttatgga ggcattatga
     9061 cttcacattt tacatggaat catatcttcc tgtttcttgt gcccgttttg attctttctt
     9121 tgattatcgg ctggtttgct attcccgaga tggcagtaca aaaaagtggt tatttggatt
     9181 ggataagcgt tttaggaatt tgtcttttgt ttagtggaag tctaacattt ttgagtacgt
     9241 tgggaacagt ttccagctgg attgctttaa tcatcggcgg tattggctgg ttcctttttt
     9301 actatcgaag taaaaaagtg gagcatccac tgatccgact gtctattctt tcaaataaaa
     9361 cattccgctt gtttttgatt ggcttcttag tttatcagtt tttattactt ggtgtatctt
     9421 ttgtcttgcc gaatttcctt cagatcgttc aaggaagtag tccttttgtc gctggattag
     9481 cgatgcttcc cggcgcagcg gttggtgctt tgctttctcc tttttccggg aaaatgctgg
     9541 atcaatatgg tccgaagaag ccaatttttg ctgggctgat tctttctttg attggttggc
     9601 tagcattatt cctactcata ggacacgctt cagtgggtgt attgattgct tgtcatgtgt
     9661 tttatatgat cggtatcgga ttatcttaca gcaatatgat gaccaccgga atgaatgcac
     9721 tgaatgaaga actacaagga gacgggaacg caatgttcaa taccttgcag cagttttctg
     9781 gcgctgtggc tacttcatta gtagcggtca ttatcaatta cgtccagcat catacttccc
     9841 ataactatga agtgtctact acattaggat caaaagcagc attaggcgtc ttgttgctgt
     9901 tgcttctcgt aagctttgct cgattcgcct attatctgtt ctttgctaaa aaagcgtaga
     9961 ttccatatac ggcaggtgtt gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa
    10021 aggaggcttt tttatggaaa aaattgccat tatcggtggc gggatcattg gtatgactct
    10081 agccagttac ttagatacat caaaattcaa tgttgcttta tttgatgaag ggacaggaca
    10141 ggcaactaaa gctagcgctg ggattatctc cccttggttg tcgaaacgca gaaacaaaca
    10201 atggtatcgt ctggctaaag acggcgctgc ttttttccct aaacttataa aagatttcca
    10261 acttgatcaa acgatttacg cacaatcagg gacacttttg cttcgacctg aaaaagattt
    10321 agctgaattg gctgttcttg ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat
    10381 cgaatggctt tcacctgaac agacatctca agcacttccg ttattgaaac cagcagtttc
    10441 actgcgtatc agtggtggtg gtcgtctgga tggaaaagca tatttagaac atcttggtca
    10501 gcgtttagca caaaaacaaa tggagcagat ccaggcaaaa gtcactttgc ataagaagag
    10561 cgattcttgg ctcatcaagg gaccgtttgg agaatacaca gctgatcgtg taattttgac
    10621 acctggcccg gcacttaagc ctttattaca agaattaggc tatgctgcag atatacgtcc
    10681 gcaaaaaggg cagctcttag catttcagac atcttataag atgagtcaag actggcctgt
    10741 tgctatgctt gacggagaag ctgatttcat tcctttcatg gatggaaaga tccttttagg
    10801 tgctacccat gaaaacgatc aaggctggga tttggctcct actagcgaag cttataaggg
    10861 attgactaag agtacagaac agttcttgac tgatccagat cagataaaaa actggccata
    10921 cgagttccgt gtaggtaccc gtgcttatac gtcagatttt tccccatttt tcggcgtact
    10981 gccagatgat ccaagcttac tggctgctag cggtctaggc tcttctggct tgaccacagg
    11041 tccttatatc ggctatctat tagccaactt cttaaatact ggtcattggt ctacggaaga
    11101 ttatcaaaag gacatctcca cctatattac gaaaaatcca tcatagaaaa acagcttttt
    11161 caagtaagga tcccgagcga tattgcgcat ggattacttc ttgaaaaagc tgttatttta
    11221 gttcaatttc tgaatcaaat tctccaaact ttcttttgct tcatctggca agagatcttt
    11281 tcctatctga gcttcaatga atcgtaaacg aatatcaagt cgttgtttca ccagattttt
    11341 ataggacgta tcctcaaaag aaacaggaaa atctggtata ggtaaatagt ggatgtcttc
    11401 aaatggacca aacgacttga attctgccgt ttgttgcacg attctttcaa cgattgacaa
    11461 tgtcgtttca ggtttgattt ttttatcatc gaaatatcca gtattcagga catagcaggc
    11521 tgtttgctgt ttcaaaaaca actctcgaaa agcttgatag tcttccctaa gaggatagac
    11581 acggaaagga ttagcaaatg gttcaatgac tagctcatct agctgtcttt ttgtagtgat
    11641 attttccgcg tctgatcgtt ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg
    11701 gtcattgatc ttgataattg gcggaagcga atcatctttc atgatccaaa aaacagcatc
    11761 gatcttttcg ctaagatgat cgactcggtc gggtgtcgca taacgtgatt tgactgtccg
    11821 cccatttccg tttcgtatat ctcctgttac taggactttt tctcctttac ttgtacatgt
    11881 cacgcccaca ttctgacaag taaggaaaga agaaatggcc ggatcatcag acggatagtc
    11941 ttgtgtttta tcaaagtaag aaggttccaa ggcagtagca aaaccatttt ttcgagaaat
    12001 aacgaaagca tcgtcatgca agatcgaaac agagtgacct tccgtatgtt ttgctaaggt
    12061 aatcgttgat tttccagaac cggacaaccc aaatacagcc atcgtatagg tctggtcatt
    12121 actagcatat tgtttcatcc caccgtggca ggcaataaag ccattgcgat ttcccaatgc
    12181 ccaagcaagc gtcaaagtcg ctttttttaa ttctccaaag taacgtaatc ctagcacagc
    12241 cgctgtatta tggagagcat caaacaaaat gagtccattt ggaaatgttt catgtgtcca
    12301 ttcgggatca caaaagatat aaagatctcc ctcatcaaag gaaacagact tttgataagt
    12361 ctctaagatc ttcgaattcg ctggctgaaa attcagcaaa taagaataca aattgttctc
    12421 aaatccttct ggaatcagca aatgactttg cacagaaaat tcttcagata ggccaacgat
    12481 tacttttgta tggtaaaact ggcgcttgcg actttgatac acagcttctc ttaaaatttt
    12541 ctcgtatttg ctttgttcct tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct
    12601 tcccacaatg cttccatcat tgaatactag tgtcctaaca tcagctggta aatctagttc
    12661 ttcagcatgt gagattgtta aatctgtgat gatcgttcct ggactccctt ttgctagttg
    12721 ataagctgtc ttcctgtcaa aaacttcatg aacattatta ccgtaaaatg tcgtttctac
    12781 aatcgatcga atactggata acagtggatt tccacttgtg atttcgttta attcgagact
    12841 ctcttttgta ctcatggctc ctcctcattc cctaaacaaa aaaacgtttt ctttattata
    12901 cattattcga gtttttattt gtactgctgc gctgtgggaa gcaaacgagt tatttttcac
    12961 acaaaaaaac cgagaacttg aataaacaag tttctcggtt tcatcatttt ccttttatcg
    13021 gcttagtacc agccgtttgc taaccagaat gttttagcag catcccatga accataacga
    13081 gatgttacat attgttctgc tacacgttct tgattggcag ctgaatagtc gccgtttaag
    13141 tatgaagcat ctaattgata acggccgatg taacgtccgt tagtagctgt gtatgaaccg
    13201 cctgattctt tttgtgcgat ccattctttt gcagagcttg aagttggcgc tgttgcagca
    13261 gctggggcag caggagctac ttcaactgtt tcaacaggtg caacgtatgt atgttcttgc
    13321 gttgctgttt cagttgtaga tgctgtattt tctgttgttt tgttttctgt tgttgctgaa
    13381 gaatcatttg aagttgggat tgttaattgt tcaccaacat aaatgcggtt gatgtttgcg
    13441 atcgaatttt tttcagcaat tgcatcgatc attgagttgt ctcctgcaaa tttttgtgag
    13501 atcttagata atgaatcacc tgattttact gtataaactt catcagcgtg agcatttgta
    13561 gttcctacaa acattgcagt agcaccagca gcaaaagttg tacctaaaac aattttctta
    13621 aatgaattca tattaaactc ctttttttct ccgtttttta ttctttcgac gctattaact
    13681 ttatcattta
//
LOCUS       DAZULO010000069        13399 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000069 DAZULO010000000
VERSION     DAZULO010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.070"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..713)
                     /locus_tag="ACG8OA_002029"
                     /pseudo
     CDS             complement(15..713)
                     /locus_tag="ACG8OA_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            792..968
                     /locus_tag="ACG8OA_002030"
                     /pseudo
     CDS             792..968
                     /locus_tag="ACG8OA_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1144..1713)
                     /locus_tag="ACG8OA_002031"
     CDS             complement(1144..1713)
                     /locus_tag="ACG8OA_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4976795.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1787..2110)
                     /locus_tag="ACG8OA_002032"
     CDS             complement(1787..2110)
                     /locus_tag="ACG8OA_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976796.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2264..3076)
                     /locus_tag="ACG8OA_002033"
     CDS             complement(2264..3076)
                     /locus_tag="ACG8OA_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976797.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3403..4278
                     /locus_tag="ACG8OA_002034"
     CDS             3403..4278
                     /locus_tag="ACG8OA_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFS4976798.1"
                     /translation="MSKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4292..4615
                     /locus_tag="ACG8OA_002035"
     CDS             4292..4615
                     /locus_tag="ACG8OA_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976799.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4612..5364
                     /locus_tag="ACG8OA_002036"
     CDS             4612..5364
                     /locus_tag="ACG8OA_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFS4976800.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5354..7525
                     /locus_tag="ACG8OA_002037"
                     /pseudo
     CDS             5354..7525
                     /locus_tag="ACG8OA_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7637..9808)
                     /gene="copB"
                     /locus_tag="ACG8OA_002038"
     CDS             complement(7637..9808)
                     /gene="copB"
                     /locus_tag="ACG8OA_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFS4976801.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9828..12014)
                     /locus_tag="ACG8OA_002039"
     CDS             complement(9828..12014)
                     /locus_tag="ACG8OA_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4976802.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="ACG8OA_002040"
     CDS             complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="ACG8OA_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFS4976803.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12236..12676)
                     /locus_tag="ACG8OA_002041"
     CDS             complement(12236..12676)
                     /locus_tag="ACG8OA_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFS4976804.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12750..13289)
                     /locus_tag="ACG8OA_002042"
     CDS             complement(12750..13289)
                     /locus_tag="ACG8OA_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4976805.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3765 a         2807 c         2381 g         4446 t
ORIGIN      
        1 gaggggggga ttttttatcg ttgtggaata tttaaatacc aacgcttgtc atgaaaatct
       61 tgtgctccac ccttagtatt tccttctgga tcgtttgtcg cacgcatcat gacgtatact
      121 ttcttattag ggaaatcacg catattgaaa gatacatgat agccaacatt tcctaaagta
      181 ttataagctt gatttacgtc tggtctataa atcccatcag ctcttactct agctagttct
      241 tttccagtat tgtagtccat aatgaaaata tactcgtatt tataattagc aatgtgccat
      301 ccagctacat gcaagtttgc gttttcgatt tctccgaact gatcaatgtg ggcgtaattt
      361 gttccatctg tcagcgtaga atttgcagca cctgctcgag ttggatcaat tactggttta
      421 tcctccgaag tagttggatt atcatccgta aaaccatgag ccaaatcata ggctagtttt
      481 tctttactta cacccatttc agaaagataa ccgtaaggat ctgtatgatc gccccaaata
      541 ttttgtgtta cccataaatg cgatttgatt cctggttggt tataaggagt gtctaatgtt
      601 aatggaatac catatttcat tgctgaatct cttgccaatt caaagtatac tttatagttc
      661 ttttcaaaag tttctttatc atgtgtatgt tgtaactcga tttgcacagg acttttggct
      721 cacgaggttg tcctactcta catcttgctg atagatatgc tgaaaggaaa acaccaacat
      781 tatgttttgc tttttcagct agaacgagac aagcaggaat acctttagaa gatatcaaag
      841 gttttttagg tcataaagat gtttccacta cacaagtcta tgcccatatt tctcccgaag
      901 tgaaaaaaag atcaatgaat caactagaaa actatataga agagcaaata aaaaagcact
      961 caaattgagt gcttttttaa gctcactatc actaaaacta tcactaattt tatttataag
     1021 cgaacatgtt tctatcactg attttttaaa tctagcgaaa aacccttatc ttaaaggcgt
     1081 tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc ctagaagtgc gaacatggtt
     1141 ttcttatagc tcatgtagaa aaattttctt gatgatgatt ttatccatca attgagattg
     1201 actgttttgc tctcctatga tttcagctat gattgtccga ttttgcggtt ttcttcttaa
     1261 ctggtagttg attttcccag tttccatcaa ctcgctaagc atatccacga ctccgccaaa
     1321 tccggtcttt ttcgtaataa aacctagtgc taccattcgt tcatctagat tttttgctgt
     1381 aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg gccagctctt ccatcccttc
     1441 agcattggat acgacggaag ctgttttgtt aataggttct tcgtttattt cttttggttc
     1501 tttcctctcc actttcactg tttgacgaag gacatctagt tcttgcaaga gcgtttcgta
     1561 ttgttcttgg gtcagcatca cgccagctac tttttcacga ttaaatatat atacgccagt
     1621 tcccgcgtct cgcgcttgag caaatacatc catcggcgat cgtttcactt ctgtgatcga
     1681 agatgtggga acttctaatt tctttaattc caatggattt cctcctttac taatcgcttt
     1741 ttattatagc acagactgac ttgttttgat cttttttcca aaggttttat cggtttttgc
     1801 ctaaccagta attgatatcg gtaagaagtc tttcgatacc aaatgaagga tggtacagat
     1861 acgttttttg atcttcgtta tggtctagtg gaaaaaaatg atcgatgatc actagatctg
     1921 cataccgagg cggcttattt cgagtacata catgtataga gatccgtttt cgatgtctta
     1981 gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg gtgttcatag cgaaaaaaaa
     2041 agacgatttt cacttcagga gggtggacat ctaaataccc tcgttcctgg agtgttttca
     2101 gtaaaagcat cccccactct actgcggggt gtgcaagtgc ttgttggatc gctagccgaa
     2161 attgatatag tttatctttg gaattgggaa atttttctaa tgtttcctga taataacgaa
     2221 cttcttctat cagccggtca tggataccaa ttacgtaacg aagtcattct tggtaaagta
     2281 cttttgtcag ctttgatttg ctttcgctct caaattcgct attcagccaa aacagcgaat
     2341 caagcttttc agtcacaatc gtcaatttcc gatagtcttg tgtctcttct atccgtttcc
     2401 accgcttttc agtaatctgg ttacagctca agcgcaaaat tgctagataa tttttttctt
     2461 ttattggcaa aatctgctcc tctctaaata gcaattcagg aaacaattca aagaatagtg
     2521 ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt tctttgcttc aataattgga
     2581 tgaacatctc agaaaaggac tgctctccat catctgcaaa aaaatgcgga aaatctttca
     2641 aaaagcaaaa cagcagatat cttacttttc tttctgaacc aaagatctgt gcttttttat
     2701 aagaaaaaga aaggtggtac tcgccaaacc actttctcaa tcgctcttgc gtagaacgat
     2761 cgtacttctg ctcgtaaggt tcattcaata gcagattttt cagtaaacaa aacgggggtg
     2821 attggatcag aaaaacaaac aagagataag gttcgctata tccttgatca taaactaact
     2881 gaacggtttt cttatgaaga atcagctgaa tgggacagtc gctttccgcc aaacgtttca
     2941 gatgccgatc cactgtttta acatcgattt tcagttcttc agctaaatca gcttttcgcc
     3001 agatcaaatc tttcattaga ttgattagtg aaaataaatt ctgtgcattt tttgataata
     3061 gtttttgcca tctcatcgat catttctcac ctgatttcta aacatattgt ttgatcaaaa
     3121 tgggttcact cctaaaaaga taaaaaactc cctttctatt tatttaaata cgcaaatctt
     3181 cctcattatt ttttatttta ctcaatttaa tcgtattctc atttcaaaac gaatgtactt
     3241 taaaaaaaca taataagatc actttgcttg tatgatgtat tcccttccat tacaatgaaa
     3301 taaatagcga ttttatgaga aggtgatgtg aaaatggaag aaaaaataga agaatccaaa
     3361 caaaaaatcg atcagttgat ataagaatta gaaaaagcgg tcatcagcaa acgttcaact
     3421 atccaattga tgatcgtggc cctattagca ggtggtcatg tcttattcga agatattccc
     3481 ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag ctgtacaggg aaccttcagc
     3541 agagtccaat gtactcctga tctgctgcct agtgatattc ttggtttttc cgtatacaat
     3601 tcacaatcaa aagagtttga atttcgtccg ggaccgatct ttaccacgat cttgttagca
     3661 gatgagatca atcgtacgac tcctcgtact cagtcagcac ttttagaagc aatggccgag
     3721 aatcatgcaa cgatcgacaa caatacatac cctctagaca atcatttttt tgtcttagct
     3781 acgcaaaatc caatcgagta cgaaggaaca tatcctttgc cggaagcaca gctcgatcgt
     3841 tttttattcc gactacaaat cggttatcct tcttttgatg acgaactttt gctacttttg
     3901 gataaattcg aaaaagtgtt ggataactta aatgaagtat tgaacagtta tgaaatagaa
     3961 gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg aagtggcatc ttatgccttg
     4021 cagttagtgt cagcaacaag aagtcatgct gccatccagc ttggcatcag tccacgagga
     4081 agcctttcct ttattcaggc agcaaaagct tatgctttaa tccatgggag aaattacgtc
     4141 acgcccaaag atttgcagga tttagttcct tacgtgtttt cccatcgcct gattttttac
     4201 gatcgttcat tgaaggaaga cgaaaagaaa caaatcattc atactcttgt tgcacaaatt
     4261 cctattcctg tgaggtagat tgaggtagac aatggaaaaa atcaaagatt atttcattaa
     4321 ttttgtgtta tttctggtat atttcatcat tgtgcttttt gctcttgtat tcaacaattc
     4381 tttaggctgg ttcttgttgg ccttttttac tctttatgta tcatggagct tgatttcatt
     4441 aagcagcttt ctaaagaaac tttcggttac ttgtacattg cagccactcg tttatcgcaa
     4501 aaaaagtatg caggttattt tttccatagc aaaaaagaga actttttctt tacctctccc
     4561 tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa aacaagagat tttgattctt
     4621 acaaataaac cgcaagaagt ccaattgctt tggcagccaa aagaaagagg atgccatgaa
     4681 acattaactg tcatttttac agcctatgat agtctaggac ttttcagaaa aagtgcaaaa
     4741 agagagatct cttttcctgt gactgttctg cctgcttttt gtaagattca agcagaaaaa
     4801 ttacagagag ttctggaaaa aaaacagcag ctgaacgcgt accaaagagg tcttgatttt
     4861 cgggaacatc gcgcttatcg tccaggtgat tctttcaacc gaatcgattg gaaattgtct
     4921 gctcatactc aagagtggtt ataccgtgaa tacgattatc aagaagaaga atgttctcct
     4981 cttttttgct tttggggttt gcccagtcca aaatttgaac ctctgctaga cttgtatttt
     5041 tctttatggc atttgagaaa agaaaagcag ccggaacttc tgatattagg caaccgcgcc
     5101 tttcatggca aagaccccgg tcatacctat tttgcttctt tggaagctgg cgatgagaaa
     5161 gcttttttcg cacacttgaa aaaatatgca aaaaaacaaa tcgtcctctt tgttcctgag
     5221 aattctcctg aagtctcaga agctattgaa acattgtcaa aagatcgaac ggtttatctt
     5281 gtttatttcg tagaaaaaca actcatggtc caagaaaaag acaagatatg ttcattgcca
     5341 ggaggtgaat ggattgacga ttaaacggga aaaatggctg cgtggcgttt actcctttgt
     5401 gatactaagt gtaggtgctc cgatatttgt agtagctaat caattgcaaa acagctacct
     5461 tcttatccta actattggtg tcctatgcct tattgtctgc tgtattccta aaaaatgggt
     5521 acgtttgctg ctttctttgc cagtaatgat aggttgtttg tttttatatt ttccttcgaa
     5581 gacattttcc atattatgga tcttacagtt tttttcgtca gtcacagagg aattcttcca
     5641 tctatcccaa ggtaaacttt attatttgcc agaaaagaca gctttttttc tcataatgtt
     5701 gctgatctat ttatttatta cattgaccgt ggattacgac tattggtacg caccctttct
     5761 ttctttgatg gcttacttca tgatgcttac cgtttttcgg aaaaaagatt tactgtacga
     5821 aatgattgcg gtagtaactg ttttgttcgt gtatctggca gcaaggcaat ttttctccat
     5881 ccggttgttg tcgggaaaca ctcgattcca gtcattgact acagttctgc ttatttgttt
     5941 cttaactttt agcgctatct tgcctttata tcttccgaaa gtacaccaat ctctcgagga
     6001 aacgagtgaa ccgatccgag aatatcttaa tgatgaaggg ctttatgaca cactccatga
     6061 gtatcaatta accggaagtg caagaacagg cttcagtgag aatgatgaac agcttggtgg
     6121 acctttatat gataatggcg aagttgtttt tacagcgaac caaaaaggaa atggctattg
     6181 gaagatcgaa aataaaaatc attacaccgg ttctggatgg atagaagatc aggacgcacc
     6241 catccaacca atccaagaat tccctgttga catcacgagc taccaaaccg ttatagaaga
     6301 caaagaaaac attacattga ccctaaaaaa acaagttttc ttaccttatc cttatggaaa
     6361 tactaccttg actgattttt cattagctag tggacaaacg ctttactatt tgcctgaaag
     6421 ccagcgattc attcctgtcc cgaatgaatc gacacgttct gcaactttac aaacagaaag
     6481 gttagttttt acgagagaag aattaaaaag cagtcagtta aaagctgatg atttagaaaa
     6541 tcagcagaca aatcttcaat tgccaaaaag tttgcctaaa cgtattgagg acttggcggt
     6601 cgaattgacc aaaaatgaag caactttaat tcgtaaagtc gaagctgtct aacagtattt
     6661 aaaaacaagc ggaaatttcc gctattccaa aacggatacc gggcatactc caaaagaaca
     6721 cgattatgtg gattatttcc tctttgattc aaaagtcggc tattgtgaca acttttcaac
     6781 ttctatggtc gtccttcttc gaacacttgg tatcccagct cgctggacaa aaggttttac
     6841 aaatgggctc agaacaggca cagataatca agggaatgag gaatacagca ttctcaatag
     6901 tcatgctcat tcttggccag aggtatactt tcctggattt ggctggcttc catttgaacc
     6961 aaccccttca tttgccaatc caatgggttc acaaccagtt gctgaaacaa ccgaaaccgt
     7021 ccagcctgct atcgaacaac caaatgaaag cgaaaatatg gcagaaacta tttccagtga
     7081 gagatctgag gtcattccat cagaagaaac aacactcaca ccagcttata aaatcgtctt
     7141 attcagcctt ggagtgtgtt tatccgtctt gttgttcttt ctattgttat tcagaaaatc
     7201 tctgcgctgg acaagttatc ttatctatct tcattttttt gcttctacta atacgattgc
     7261 atatcaaaaa cttcttaacc ctttggaaac attggtaccg cgcaaacccg cagaatcttt
     7321 gcaggaatat agcctgcgaa tagaacaaga attcaagcta gatctgcgtt tcattcgatt
     7381 aactactcaa tatgaagcag aaatatatgg acgacaagca gatccggtaa aattcgataa
     7441 aaaaacctat ttagaaataa cgcatataat aagaaaaatg gtatacaaaa aaattctgac
     7501 aaaagggagc cgcttcaaaa aataagataa aatttctgga gaatctactt ctggaatgcc
     7561 gagaaccagc tgattgagta tcgattactc gaaaaaaagg ttgtgacacg ttttttgtca
     7621 caaccttttt tcattattat ctgattttca atgtcaaagc atttagagct acaactaccg
     7681 tacttaacga catcaagaca gcaccaactg ctggacttaa tatgatacca attggcgcaa
     7741 gaattccggc tgctagcgga attgcaagaa tattgtaacc tgccccccac cagaggttct
     7801 ggatcatttt ctttcgcgtt tgcttggcta gatcaagaaa atgcaaaata tcttttggat
     7861 cgctgtttgt caatacgaca tccgcagaat cgattgcgat atctgtccct gcaccgattg
     7921 ccatcccgat tgaagcacgg gctaaacttg gcgcatcatt gatcccatca cccaccatga
     7981 ttactttttt cccttggtca aggtatttct ggacaacagc ttctttatca tctggtaata
     8041 aaccgccata atattcttcg atccctaaat aatctgctac tgcttgggca gcgtttttat
     8101 tgtcaccagt aagcatgaca ggagtgattt ttctttcttt caatgttcgg ataaattctt
     8161 ttgcttctgt ttttactacg tcacctaaag caacgattgc agctagatga ttttctaaaa
     8221 tcaggaagct gatggtattt ccttgttctt ggtaaggttt caataatgct tgttctacgt
     8281 ttaatccgag acgctcaact tctttttcat taactatttt cacttgttgt tttttgactg
     8341 ttgcctctag cccgactcct gaaagatttt ttagattttc agcttgataa ggcttgattt
     8401 catgttcttt caaataattc ataatgccaa cagccaatgg atgattggca ttcgcttcca
     8461 aggctcctag atattgaagg atttcatttt tattgaacgc atcatcaagt acttcgacac
     8521 ccgttacagt aaattttcct tctgttagtg ttcctgtttt gtccagcatg atcacatcta
     8581 gatcattggc ttgctccagt gcattgcggt ttttcaaaag taatccgttt ttagctgcta
     8641 ttgatgtgga acgagcaaca actaatggga tagctaaacc tagagcatga ggacaggcga
     8701 tgataaagac tgttaccata cgttctaatg cattaggaag atctgtcaag aacaaccagg
     8761 cgatgaatgc caggataccc acaattaatg cgacataaaa cagccatttt gccactttgt
     8821 cagataatga ctctagcttg gatttttctc cttgtgcttt tcgcaccatt tccataacct
     8881 tcgctagata accattttca ccagttccag ttacttcgat ttcgatcgtt ccatccccat
     8941 tgatagaacc gccaatcact ttgtcattgg cttgtttttt gattcctctt gattcgccag
     9001 tcacagctga ttcgtcaaca attgtttcac ctttcaagat tacaccatct gttggcattt
     9061 tatcaccaga ccggacgatc aaacgatcac cttcgtttac ttctttcaat gaaactgtct
     9121 cttcttttcc atctttcgtc aagcgtttca ctgattctgg cagtaattct gctagttttt
     9181 gtaaagcatt gctggcattc gacacagcat tcatttcaat ccagtgacct agtagcataa
     9241 taacgatcaa agtagccaat tcccagaaga aatccatgac gtgtgtatgt ggattgagca
     9301 gatttgcgat aaacgaatag acactataaa tatatgcaac tgtaatcccc atcgcgatca
     9361 gtgtcatcat agcaggactt ttttgtttca attccatctt agctccactt aaaaatggct
     9421 gaccgccata gataaatagt atagttgcta agatcaatac gagccaatca gaaccaggaa
     9481 acgttacttg aaatggaaat tccattccca tcattggtga aaaaagaatg attgggatag
     9541 ctaggataag cgatagccaa aatttttgtt tgaaattccc catgtgcatg gaatgatcca
     9601 tgccgccgtg ttcgtgatcc atgtgcatcg aatggtccat ttgtttgctg tttgctatat
     9661 ctccatggtt atgtcccatg tgcatggaat gatccatctg actatggtca tgtttcatct
     9721 ccatctgttt ttcatgagat tctgtacttt ctttgtttac tacttctttt ttatgctcat
     9781 gttttttctc catatcgttc ccattcattt ttactccatc ccttctctta ctttttcatg
     9841 tgtcgattta agcacaggga gttcaacaat acactgacgg aactaaacgc catcgctcct
     9901 cctgcaatga ttggattcaa aaagcctagt gcggcaaaag gaataccaat cgtattatag
     9961 acaaaagccc agaaaagatt ttgtttgatt tttctcaatg tcaacctaga caactcgatt
    10021 gtttgtgcaa tggatgccaa actgctattc atcagcgtca catcggctgt ttccatcgca
    10081 atgtccgttc cacttcccat tgccataccg acatcagcta atgctaaggc cggtgcatca
    10141 ttgattccat cgcctgccat accgactttt ttgccatctt tctgaagttt ttctacgtag
    10201 gctgctttat cctctggaag tacttcagca aagatatgct ttggatcaat accaacttgt
    10261 ttaccaatcg tttcggctgc cagtttgttg tctcctgtca gcataaaaac atctaatcct
    10321 ttattctgga gctgcttgat tgcttgtttg acttctagtt tgatttgatc agccacggca
    10381 atcaacccga tcacttgctt ttcatcagct aaaaacatga ctgtttttcc ctgctgttct
    10441 aattctaacg cgtgttcttg gtattcatca aatgataggt tcaattcaat taatcgttta
    10501 cgtgtacccg caaaatagcg tactccgtta atcgtaccgc tgattccagc acctgggtgg
    10561 gctgtaaaat caatcatatc gtatgttgct gcttcttgca gttttccgta ttccacgatt
    10621 gctttaccta gtggatgttc agaagcttgt tctaaagtat aaaaaataga taggacttct
    10681 ttagaaccga ctagatctgt aactttgggc tttccttctg taatcgttcc agttttatcc
    10741 aaaacaatgc tgtctaagtg agcagctgct tctaatgctt ctccgccttt aattaaaatt
    10801 ccatttcgtg caccaacacc cgttcctacc ataatagctg ttggagtcgc taaacctaat
    10861 gcacatggac acgcaatgac taatacagag acactatgga tcaatgcgag ttgccaatca
    10921 ccagtgatca aacctgtagc tatgagcgtg ataaacgcga tgactaatac gatcggaaca
    10981 aaaatccctg agatccgatc tgcgattttt tggattgggg ctttgctccc ttgagcatct
    11041 tccaccattt gaatgatttg cgctaatact gtctgcttgc cgacttgtgt aacttccatg
    11101 tgaagtagac cattcgtatt gattgtcccg ccaaagagcg tgtcatccgg attcttttct
    11161 acaggaagat tttcaccagt aagcatactt tcatctatcg cactgcttcc tgaaataatt
    11221 cgaccatctg ctggaatctg ctcaccagga cggatgacta gttgatcacc aacgactact
    11281 tcttcaatcg ctaacgtttc ttctttcccg tttcttatca cttgggcggt ttttgtttga
    11341 agagacatca attgtttgat tgcattacct gttttcgttt ttgctgtatg ttccaaatat
    11401 ttgccgagca aaatcagcgt gatgatcata ctgctgcttt caaaataaag atcttggggg
    11461 tgtccacgga aaaatccgtt gtaaatgctc aatgcaaagg cagccgatgt tccaattgct
    11521 accaatacat ccatatttgg tgctttcgtc ttgattgcat ggtacgcacc tttataaaaa
    11581 cgagcaccga cataaaattg gacaggagca gacagcacta actggacgat tggaaaatga
    11641 aaaaagtgaa caatcgccgc atggcttcct aacatcatag caatcattga caacatcaac
    11701 ggcaaagtca aaaccgtact caatataaga tcgaatagca tttttttcaa atacgcttgc
    11761 ttttcttctg caattttttg tttgtgggct tcatcgtaca aaattgcacc atagccgatg
    11821 ttttctacgc tttggattaa tttttcagtg gtcgtccctt catatttgac acttgctttt
    11881 tcagtggcca agttgactgt ggcattgacc acaccaggtt gtccgttcag ctctttttcg
    11941 atcctagcag aacaatttgc acaagtcata cctgtaatga cgaacgtatc cattttggtt
    12001 tctgctgttg ccatcaaatc acctctgctt tataacccaa tccattgatt gcttgacaaa
    12061 tcttttcgga ttgaactttt gtttcatcaa acttgaccgt accgtttgct tttttcaaat
    12121 ttactttcac tttttgcacg ccgtcaagtg cgcttactgt ttcttctact cttgctgcac
    12181 aatgattgca gctcatacct tcgattgaaa attgttgttt cattttttct tcctcctatt
    12241 gatgttgttt acattcacat tgacctggga tacagttaca ttcgatcgta tctactggtt
    12301 gcttttgttc cagttgtttc aaaatctcat tgatatcttc ttgagtcaaa gtggcttctt
    12361 ctactagatc cgcaatcgtc tctccaatcc tttttgcaca gatatgggaa aaaagatttt
    12421 ccgtcgcact gcgaactgtt tccatctccg agacagctgg ataataaatg aatttttttc
    12481 cttcttgttc tgtccagatc acttcttttt ttaccaaacg acccagcaaa gttttgatcg
    12541 ttgctggttt ccaatccatc gaatcagcta agatctgcgt gatttcttgg gctgtcgtct
    12601 gtccaagtgt ccaaataaca cgcatgatct cccactctga atcactgatc tttactggcg
    12661 ttttgacttt ttccatttat ttcacacctt tcgtttacaa atgtaatcat tatcttatga
    12721 atacagatta cacttgtaaa caaaaactgt caattatttt tgctctctat tttcaattta
    12781 gttaaaaatt cttgtttctc tcgtcttgtt ttaaggatga tcagtttatt tcttgcatta
    12841 gctacttgga tcagctgctg tattttcggt tcattgtttt cttcaaaatg ccagataaaa
    12901 gataaaaatt ctaagtattc cctgtttagt ttctccgaaa atgattcagg catatctggc
    12961 cgagattttt tgtttatctt tctttttaga gaacgccaaa ctactcggta gatggcgaga
    13021 taacgcggca ctttaagcca aatgatctta tctgcttctt taatcctttc ctctaatgta
    13081 gaagtataat ttccttcaac gatccatgac ggattagata ccataaacaa ttgctgttct
    13141 tgcaaaaacc acttttttgc ctgaatactg tagtctgttg tatgccataa aaggtctagc
    13201 ggcagtagcg gaaagtcggt gatttctttg atcttccttg cgagggtcga ttttcccgac
    13261 ccactttgtc caatcacgat gatcttcatt cttctccctc acttctcttt ggaatttggt
    13321 tcgatcatac cacttataac gccgcaaaaa aacaagggac tgtgacaaaa gtcgtgtcaa
    13381 agtcccttgt ttcgaataa
//
LOCUS       DAZULO010000070        13356 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000070 DAZULO010000000
VERSION     DAZULO010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.071"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            7..174
                     /locus_tag="ACG8OA_002043"
     CDS             7..174
                     /locus_tag="ACG8OA_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFS4976806.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(236..2047)
                     /locus_tag="ACG8OA_002044"
     CDS             complement(236..2047)
                     /locus_tag="ACG8OA_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFS4976807.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2044..2409)
                     /locus_tag="ACG8OA_002045"
     CDS             complement(2044..2409)
                     /locus_tag="ACG8OA_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFS4976808.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2572..2967)
                     /locus_tag="ACG8OA_002046"
     CDS             complement(2572..2967)
                     /locus_tag="ACG8OA_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS4976809.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2985..3947)
                     /locus_tag="ACG8OA_002047"
     CDS             complement(2985..3947)
                     /locus_tag="ACG8OA_002047"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFS4976810.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3947..4159)
                     /locus_tag="ACG8OA_002048"
     CDS             complement(3947..4159)
                     /locus_tag="ACG8OA_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFS4976811.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4180..4878)
                     /locus_tag="ACG8OA_002049"
     CDS             complement(4180..4878)
                     /locus_tag="ACG8OA_002049"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFS4976812.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4898..5440)
                     /locus_tag="ACG8OA_002050"
     CDS             complement(4898..5440)
                     /locus_tag="ACG8OA_002050"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFS4976813.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5572..6576)
                     /locus_tag="ACG8OA_002051"
     CDS             complement(5572..6576)
                     /locus_tag="ACG8OA_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS4976814.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6573..7562)
                     /locus_tag="ACG8OA_002052"
     CDS             complement(6573..7562)
                     /locus_tag="ACG8OA_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS4976815.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7559..8365)
                     /locus_tag="ACG8OA_002053"
     CDS             complement(7559..8365)
                     /locus_tag="ACG8OA_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4976816.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8531..9487
                     /locus_tag="ACG8OA_002054"
     CDS             8531..9487
                     /locus_tag="ACG8OA_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4976817.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9564..10082)
                     /locus_tag="ACG8OA_002055"
     CDS             complement(9564..10082)
                     /locus_tag="ACG8OA_002055"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS4976818.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10170..10319)
                     /gene="rpmG"
                     /locus_tag="ACG8OA_002056"
     CDS             complement(10170..10319)
                     /gene="rpmG"
                     /locus_tag="ACG8OA_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS4976819.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10547..10993
                     /locus_tag="ACG8OA_002057"
     CDS             10547..10993
                     /locus_tag="ACG8OA_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFS4976820.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11188..13083)
                     /gene="asnB"
                     /locus_tag="ACG8OA_002058"
     CDS             complement(11188..13083)
                     /gene="asnB"
                     /locus_tag="ACG8OA_002058"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFS4976821.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4159 a         2832 c         2278 g         4087 t
ORIGIN      
        1 gattcaatga aaaaatttgt atctggaata ttagtaggaa gtctggcaac agccgctgcc
       61 gttgctggtt tggttgcttc tgtgaaaaaa acagtgatcg atccaattga cgaaaaagaa
      121 gcaatgatcg aagaaaaccg taaaaaagca atgcgtaaac gcgtttctcg ttaataatta
      181 tttaaaaata cgcatgaaga ctttctaaaa aacagataag tcatcatgcg tatttttatt
      241 tgtcagtcaa tataagttgt gctattttat ctgcctgtct cagattccat ggatctgtat
      301 aaggtctagc tactgctgca gttagccaat ccacaccttc aacaggtatt ccatagcaat
      361 aaaagtcaga ctggatttcc cctgaagcat caatcacgcg attcgtacct cgctcaacat
      421 ctatagcttc tgaatgataa cttcctgtat tatctgaaaa tgaaaaaggg cgaacaagtt
      481 tgttttcctt caaatttttt atgacaggat tacttgaacg agaaaagctt gtttgtggca
      541 atcgagcttc aatcaaatag cgactgctga tttctttatc ttgtagagaa ataacatagc
      601 ggttgtgctt ttgctgaata tcaaatggcg gttcgatcaa atgaaaaata cccgcatcta
      661 tcagtgctgc taattctttc gtccgaatga ttggcggacc gatcgttaaa aagctattta
      721 gatgagtaaa ccatccccat agtatttctt tgtattcctt agcagaaaaa acgtgccatt
      781 caatagcctg ccggattgga tcacgccagt cttttaatgc atcaaatgtt gaagcaaatg
      841 ctcctgtgca attgcctttt tccgcttctt ttatctgccc ttcaataaat attcggctag
      901 cttctgaaaa gggctcattc tgataggcaa gaggagtttc taaatacgac caatcccatc
      961 tataagggat tagtgctgga tgctttccga gacactcctc tgaagagagg gagagaaaat
     1021 catgctcaaa atttttcttt ggaatcggca agtgatattc ttcgatgatt ttcttgtaat
     1081 aaaacaactc aacgtctttt ttcagatagt caaaaaatgt ttcgccagtc aaaaatcccg
     1141 actgatgcca ttgctccagg ttttcttttg ttagtaaccg tggccatgcc attgctccgc
     1201 cttgcttttg atttcttcct cgagggtaat aagggagccc ccttcttgat ccactgtata
     1261 caatcggttc cttgcctgat ggatgataga ccagttttcc cttttcgttc tcgaagcgcc
     1321 ctcctcttga tactgtaaac aatccgacat aatcaaaaaa acttaagcct agacctctta
     1381 aaaagacatt ttctttaggc ggtatcttcg aaagatctac atctgcagga ttagaaggaa
     1441 gctgatacag taaaacattt ttctttgcgt agtctaccca cactctttct tcttctgtct
     1501 catcattttc ccagtgaccg cttgctaaaa caagctgatc gatagaatag ctttgctgat
     1561 ctgtctgtaa aagataacca tcttttcttt tgcttacttt ttttactaaa gaataaacaa
     1621 actgaataga agctggaaat tcttcttgca actttgagaa aaaccagcgt tgatagagtc
     1681 cgtataaaca tcgagtagat tgctgatctt tttccagacg attgatttct tctataatta
     1741 gttgcttatt tttggttacc gtctgttcga tataatctgt agcaggtcct ttactccatt
     1801 gaaacaagtt gggtccctgt gttaccaatc ctccactgga caatgtttca tctgtaaaaa
     1861 gcgtcacttg ttgagaaaca gaattcatta ataactccgg tggctgattc aaccgccaaa
     1921 ctcgacctcc aggaccattg ggatcgaaaa gaaaaagttc aagagaatgt ctgtcctgat
     1981 tattatggag aatcctttct gctacactta atcctctagg tccggcacca atgatcccta
     2041 ttttcatttg catgcctcca tttcgataaa ttttttcaat aaaacagcat ggttctgatg
     2101 ttcatttttt gcaccataaa caaaagtaat gactggatgt tctttcataa gttgactgag
     2161 tttaaaaata gcttgcttct tttcttcttc gaaaatcaat tcttgctggt atttctttgc
     2221 aaatgaagca aatcgttctg gtacatggtc aaaagttttg cgtgcttcat tactaggtgc
     2281 aatttctttc aaccacagat ctaactgcgc tttttctttt gaaattcctc gtggccagaa
     2341 tctatccacg agtattctat agccatcctc tttttcagca tcaaggtaaa cacgttttaa
     2401 tttgatcata gttgtttcct tctttctacc ttttcgctta gcttatatat gtagataata
     2461 gcacaaaata aaaaaaatga acggcacaac tatccattgc gccgttcatg tgatcttggt
     2521 caaaatctaa tgattgattc ggtttttaaa gctgtcatac aatcatctgt tttatacttt
     2581 tttacttttg atttttccag accatccgtc atatccaccc ttaagtaaat aaatctcttg
     2641 ataacctgct ttacgtaatt tatttgctgt acggatgctc aaacttttct tttgatcgta
     2701 aatatagaca ggctggtctt ttcgaataga tcctaatgaa tctttcaaaa cagtgtaagg
     2761 gatgtttctt gcacctaaaa tatgcccagc atcaaacaca tctttttcgc gaacatcgat
     2821 tacttgtgct tttctcattc cttctcgaaa ttcttcttct gtcaaagtcg ttgctgaacg
     2881 tttggccatt actcgtagat agacttccca aaggatgatt gcaagcacaa tcaatagcaa
     2941 aatgatatta attacccata aaaccatgta atgcctccgt ttttttaagc aaatttcaag
     3001 gctaatgagg cagcaccaat gacaccagct tcattaccaa gctctgctaa tttgattttt
     3061 gttgattctc ttacttgcgg gaaagtaaat tctttaaaat atttctctac acggctacgt
     3121 aaaaattctc ctgctgccga tactcctcca cctaacacga tacttgacgg atttaatgta
     3181 ttccccaagt ttccgcaagc taaacctaag tagaagcata cgcgatcaac aaccatcaat
     3241 gcaaacggat cgtcttcttg agccatttca aagatatctt tacttgttac ttcttctccg
     3301 ttgtctagca ttgctttcag ttttgaatcc cctgcgtatt cttctgccaa gaaacgacct
     3361 aggcgtacca cacctgtagc gctagataca gtttctaaac atccgcgttt accgcaagta
     3421 cattcgaacc cttcaggatc cacagtgata tgtccgattt ctcctccagc tccgctcacg
     3481 ccgtgaatca aatttccgcc agcaacgata cctccgccaa cacctgttcc tagagtaacg
     3541 aatacaacgt ctggatcgtt ttctccagca cctttccatc gttcacctaa agcagcaaca
     3601 ttcgcgtcat tatcgatagc gaatttgatt cctgttcctg cctcgatcag ttttttcaca
     3661 aattgaactt ctgtccagtt taagttatac gcaccaataa ccgttccagc ttcgctgtcc
     3721 acgcttcctg gggtacccat gccgattccg ataaagtctt cggctttcat attatataaa
     3781 ttcaaacgat gattgattga ttcaatgatt tcaggaacga tatgcttacc atcatccaaa
     3841 atattggtat cgatgctcca tttttgctga atctctcctt ctggtgttaa aattgcaaat
     3901 tttgctgttg ttccacctaa atcaatccca ataatcttct tgtccattac aaatttcctt
     3961 tctttcggct ttcttcaatt cggtgctctc tttttaatat gcgccacgct gtcatgtatg
     4021 tatcatgatc aatcaaacgg ctatcataca gattacgcaa ttcgatagtc attaattcaa
     4081 tgtcatagat gcgtgcaccc acatagataa agatgccaaa ttttttgaac agctgctgta
     4141 catcatataa gctctccatc tttaagcact tccttcatat tatacaagta aaccatattt
     4201 ttttaaaccc agtaaaatac atattacaaa aagaaataca aaaactagtg tagaaagaat
     4261 cctttggtgt atcgaatagc ttgtctgttg tttcggtaga cccaaaacat tccctaacac
     4321 taatccgcca attagcccac cgacatgtcc ccaaacatct acagaggtat ccagaagacc
     4381 aaaaacaaat gtcaacacaa taaataaaag aaaatgtctg actagctgct ggatcgctgg
     4441 gttatgttta aaatgaaacc ctaagataaa caacgcaccg aacaaaccga atatcgaagt
     4501 acttgcacca ccagacagaa cattcgcttc gttaaaagca aagcttgctg tatttcccat
     4561 tatcccgctg aacatataaa tcaagaaaaa acgccaatgg ccatagattg cctcgatttg
     4621 ctgccccata aaatacagta tcagtgagtt cattacgaaa tgcatcagtc caaaatggat
     4681 gaagattggt gtcactaaac gccaccattc attgaagtga acaagataag gaccatacat
     4741 tccggcataa taagggatct caagtcgcgg aaataattcc atcatgagga agaccgctgt
     4801 ctgtatccct aaaaaagcat aggtccataa cggtcgtctt aaccatgcac gcaattttat
     4861 ttgctgttga taattcatga aaacctccca tttcatttta gtcaataaac aattgttgga
     4921 tcttctgatc aaagctctcc ggttgccatt gttcattcaa ttgttcacga aaaacaaggc
     4981 tacatgtttt tttatgaaaa ttatttaaat accggtcgta ataaccaccg ccaaaaccga
     5041 ttcgataacc ggctctattg tagataatac ctggtacaat gaggagatcg atcatctccg
     5101 gcaaatagag cttctctgat tgtggctcaa gaacaccaaa actcgaacgt acaagaacgg
     5161 actgttcatc catttcataa aacgccatct gacgtttcgg caaagtttta ggtacaacta
     5221 ttttcttttg ttctttttct gctcgttgga taaccggctg tgtatccagt tcaaattcat
     5281 tcgataaagt cgtccctatc acttttgctg acttccagta ctcgctggcg aatagctgtt
     5341 tatagattcg ctgttctttt tcctttttct tgccactttt ggccagattt ttcagctttt
     5401 cgattccttt ccggcgtaat cttactttca attggtccaa aaactcaccc ttccttctct
     5461 cataaaatgg tataggaaaa aaagaaaaat agcaatcgaa ctttcaaata aatgcataaa
     5521 tagaataaaa agaagaccta gaaatgattg gccttccttt tttaatgaat ttcaactgtt
     5581 cagcgcgtgt ttttgtctga taaaaagtgc atacagaaaa tatggcgcac caaaaagggc
     5641 aatcaaaatt cctgtaggta cttccccact tggtagaatc actctagcaa ccatatctgc
     5701 actaagcaga aaaatggaac cgaacaaacc gctcagaaca aaggtccagt tattttcttt
     5761 acgtacgact agcttcgcag catgaggagc tagtagtcct acaaaagata aactacctgt
     5821 caccgctaca ctaccgcttg ccaaagcaac agctaacaac aaaaggattt tttgactttt
     5881 agccacgtta aggcctaatc cttgcgcgcg ttcttgacca aatgatagca atacaatcct
     5941 ctgtgcataa agtcctccta gcggaattaa gataaccgcc catggaagca atgcccaaac
     6001 ataaggccag ctcgcacccc agattgatcc cgctaaccat gcattcacaa atccataatt
     6061 ttccttagat aattggatcg tgcatagcaa tgttaatgca gaaaggccgg cattaacggc
     6121 aataccggac aataagattc gattcatacc caaaaagcca tttcgatcat aggccgcaag
     6181 atagacacag caagcagcta aaacaccacc tagaaaggct acaactagca atagaaacgg
     6241 cagcgaatag tgcgaaaaga aacctaaata aagcatcact aaaaatcctg ctccggcatt
     6301 aatgcccaat atactagaat ctgccaattc atttcttgtt actccttgca agatataacc
     6361 agaaacgcca agacaagctc ctgctaataa actgatcagt aatcgaggaa ctcgaaattc
     6421 tctaacaatc agcgccaaag aagatgattc tttccctatg aacgtggcaa aaagctgatc
     6481 caggtaaata aaaggcgtac caatcattaa actacctata gacaggatga caagtacaaa
     6541 aagcaagaaa tagaacgtcc aatatatttt tttcaccgtt gttccctcct tacttgtatc
     6601 agtaaaaacg gaacgccaat caatgcaatg actaatccaa aaggtgtttc aaaaggggga
     6661 ttgatcatcc tcgctgtaaa atccgccaac ataaccaatc caccgccaac taaaaaagag
     6721 acaggtagaa cataacgata gtccgttcca accattccgc gaacaactgg cggaaccaac
     6781 agcccaataa atgaaacagg tccgactaac gaaacggcaa tggctgctaa cactgcaact
     6841 agacagaaaa caatcaaccg cgttcgttta ggatgacttc caagagctaa tgcatgtgtt
     6901 tcactcaaat gaatcaaaga tattgctttt cctaatgaaa aagaaaccaa aacagcaagt
     6961 atatataaag gaaacaatac tgctaattgt ttccatgtga tattggcagc tccgcctaca
     7021 aaccaaaaag caagatcctg ctgcagatta aagaattggg taagtgcttg gctgatagca
     7081 gtaaaaaaag aactaatagc tacgccagct aaaatcagct gtaaaggatt catgcccatt
     7141 ttcgaaaact gcaaaaaacc aaatacgagc aaagttacag caaaagaacc tgccaatgac
     7201 attagaaaca cagaaaaatc agaagcttta ggccaaaaag caaaactaag ggccattgcc
     7261 aacgaagcac ccgcgttgat tcctagtaag cctgaatctg ctaaaggatt tcttgtcacc
     7321 ccttgcatca atgcaccgct caatgcaaaa caactcccta caagaaatgc tcccgccaca
     7381 cgaggcaaac gaatatttcg tatgatttgg tgactttgtt ttgatctatc aaaatcaaac
     7441 aacgcctgcc aaaaatctgc tttttccaca gtcgctgcgc catttgaaag agcaacaaaa
     7501 aaaataatca aaagaacaaa acagaccaaa agcaggagtc tatttttctt catagtcctc
     7561 ataattgatc ctcctgctct tttggcaaca aatcataggt tagaaaaact ggtttatttg
     7621 ttcttggaag cgtgacaatc gttgcatcaa tctcaaatat ttttttcata cactcttttg
     7681 tgatcacttc ttcaggtgta ccttctgtga tcaacttacc gtttttcatt gctaataaat
     7741 aatctgaaaa acgtgaagca tgattaatgt catgaatcga catcaagatc gttgtttgtt
     7801 tcatttgatt gatttcagtc aataactgta ggatctctaa ttggtgtgcc ggatctaaaa
     7861 atgttgtcgg ttcatccaag atcaagatat ctgtttcctg agcaagcgcc atagcgatcc
     7921 agacacgctg ggtttgtcca ccagacagcg ttgcgatatc ccgcatacga aatccagcta
     7981 gacctgtagc atccaacgcc cactcaattg cttcatgatc ttgcgtgtcc atgccactga
     8041 acagtttttg ataagggtat cgtccgtaag atactacttc ttctactgtc atacctgtag
     8101 ggtgttcact agtttgtgct aataatgcaa ttttttttgc aagttctttt gaactgtatg
     8161 tagagatatc agagttattg acaatgatct ttcctgattt cacaggtagt atacgggtaa
     8221 aacttttcaa taaagtagat tttccacttc catttggccc aatcaaacta gtgattttcc
     8281 cttcaggaat cgtaacagac atgttttcaa tgattttttt gttttcgtag ccggctgcaa
     8341 tgtcagctgc tcttaatctc gtcatttcac ccatcctttc gctgaaaatc attctcaatt
     8401 atgacgctgt ttcaacattt cgtcaagcgc tactttcata tctatagcca aatgaagcgg
     8461 ttgacagcgt tcgaaatatc aggaataata accaatgaga aagattctca attatgttag
     8521 gaggaaagaa atgaaattaa aaacaaaagt attcacatta tctagcatat tgctgctggc
     8581 tggagtactg agcgcttgct ccccctcttc agataaagaa acagctacaa ctgccaattc
     8641 gacgacttca tccaatagta ctcatacagt cacagataca cttggacata aagtagagat
     8701 tccaaacgcg cccaaacgga tcatcggcag ttacttagaa gactatctga ttgcactagg
     8761 ggaaaaacct atcgcccagt ggactgttgg cagtggttcc attcaagatt atctgcaaga
     8821 tgatttaaag gatgtcccaa cgatttctta tgaccttcct tatgagaaag tgttaagctt
     8881 tgagcctgat ttgctgttga tttcttcttc cgctactgtc gaaggcggga aatacgaaca
     8941 atatagcaaa atcgcgccaa cttatgttgt aaaaaacgga acagatgtca catgggaaga
     9001 acaactaaaa gatgtcggca aagctttggc aaaagaaaaa gaggcagaaa aaatcatttc
     9061 cgattatcaa aaatccatca agaaaactcg agaagaacta gcagataaaa tcgagaacaa
     9121 gacagctgca gtgttgtggg tgaccaacaa ctcagccttt atggtcagca aaaatcggtc
     9181 aagtggaaga atcgtatacg acgatctgga atttggtgtt cctgatctag tggaagaggt
     9241 ctccaaaaaa gccacttctg actggtctgc tgtttcctct gaaaagctag ctgaattaga
     9301 tgctgactac atcatccttg tgaacagtga caaagatgct gccatgttca atgaaccaag
     9361 ctggaagaat ctaaaagcag tgacagacaa tcatgtactg caattcggac ctgaatctag
     9421 ttggctatat aacggaccta ttgccagtca ggaaatggtc aatgatatca aagaaaaact
     9481 aaattgattt taactaaaaa aagcatcttc acgattagtc gtgagatgct ttttttctta
     9541 ttaacaaact agacgcatag attttattct tcttttgaag caaccctatt ttttactctt
     9601 tcccattttt gaaaacgatg ggcatataga ttttcttgat ccgttatccc ctcatactcc
     9661 tctataaggg cgaagtctga aaaatcaata ttcccaatat acgtatcgcc ttcaaacgtg
     9721 ctatcgatca atgttcgcca gatccgacca gtgtaaggaa gaagttcttg aaaaatccga
     9781 ctaccacccg aaacatagat atcttctgag atcgtttgac tatacgacaa aagttcatcg
     9841 atcgaatgca ttacttccgc attttctttc gtgcgaaaat ttggttgcgt tgtcaacacg
     9901 atgatatggc gataaggaag tgaaagatcc cccattcctt cataggtctt tcttcccatc
     9961 actaaaattc gatccattgt atgttcgcga aagaaacgca tatcatttgg caatcgccat
    10021 ggtaacaaac catttttgcc gatcagacca tttttatctt gtgcccacat ggaaataaac
    10081 atattatccg ctccttatga ttttagatgt tttgagataa aaaaagtctc aattctttga
    10141 ccagcaagga attgagaact ttaaaaacat tattttgttt cacggtgcaa agtaactttg
    10201 cgttcgcgtg ggcaatattt ttgtttttcc aaacgatcag gattgttacg tttgtttttg
    10261 cttgttagat agttacgttc tttacaagaa gtacactcta aagtgatgtt tacgcgcatg
    10321 tttttccctc ccatttttac aagaatcctc taagatgact ctcagacttg attatcttac
    10381 catgattttt tctagattgc tagtgttttt cacgattttg ttaagtaaaa ctacttgacg
    10441 ataaatacaa cggttttact tctcaaacaa aatctttttc tttttgtata aataccctta
    10501 cacttgttta tatagattat gttttttaat aaagggggat actaatatgc catggaacat
    10561 gaaggacttt cctgcatcaa tgaaaaactt agataaattg acaagaaaaa aagcaatcga
    10621 catcgcaaat gcattattag atgaagggta ccctgataac cgtgcgatcc caattgccat
    10681 tgatcaagca aaaaaatggt atgaagatgc tagtgaatca gaaaaacgaa ctttcgaaaa
    10741 agaaaaaaat ccaagcaaga cggatgaaca cgatacgaat cctcgtgctg gaaaactgct
    10801 tgattcagat gtgatcgtag aatatgagga agaacaatgg atcgtgaaat ctaaaggggc
    10861 aaaaaaggca agcaaccatt ttgatacaaa aaaagaagca atcgaaaaag gaaagcaagt
    10921 agcacaaaac aaagaatcaa ctttgttcat ttataaaaaa gatgggacaa aagaaaaaga
    10981 aatcgattat tgaacatatg taaaggggtt gtgacgaaaa tcgtgtcaca acccccttat
    11041 gttcgaaaca atggtgttcc tctgccaact ccgaaccata agtcataata tctataaaac
    11101 gggaagagct gttttttgaa attgtttctt atcactcagt gttaaacggt aggagaaaac
    11161 ctctttttta ttgatatttg attttcatca aacagtcaca tagtcgattg cttctgcttg
    11221 cggaatcgtt tcatctacaa agtagacttt ataccatgtc aagaaactga agattgtcca
    11281 gatttttctt tgctcttctt tacgtccttc gtgatggtca tctagcaact tcatgatttt
    11341 ttcttgatca aaaaattctt ttgcaaaatc tgcttcgaat aatgctttga cttgttcgta
    11401 gccgtgttct tctttcaacc aagctttgat tggcacaggg aatcctaatt tcacacgatt
    11461 cgaccattct tccggaagat gtttgttcgc agcttgtctg aaaatatctt ttgtgttgtt
    11521 ttgattcaat aaaaacttac ttggtatttg ttgagccaat ttcatgactt cgatatccaa
    11581 taaaggtact cggacttcca aagaacttgc catagaaagt ttatctgcct ttaacaagat
    11641 atcttttggc atccattgat gaagatcgac atattgcatc ttattgactt catctttgat
    11701 tccttctgtt ttctcgtaat gaacagtcat gatttcatcc acagatggtg ctgtttgata
    11761 ttttggtgtt aacacttctg atgcttctga ttcttcgaac actcgagcat gaccgataaa
    11821 atattctttt gcaggagcaa gtgattcata taaatggatc cgtccatgga agtttttcat
    11881 ttttccgatt ttacgtccta aattatagcg tgttcctttt ggtaattttt tcaatccttg
    11941 agcaatcaca cggatgaatt ttgaattcgt atggaatcca tacgtttggt atcccgcaaa
    12001 cagttcatcc gccccttctc cagattggac tacccgcaca ctttgtgaag caagatttgc
    12061 taaaaagtac aaaggtacgc aagaaggatt tgaatcaggt tcatctaagt gatactggat
    12121 caaagggaat gctttgaacg acatgtctcc gtcgatcact gctgctgtat tatctaagtc
    12181 caatagttcg gttagttttc tggcttcaat cgcttcatta taggttttat catcaaaccc
    12241 gattgagaaa gaatgatctg gtttcaatac agaggttaca taactggaat caaccccgct
    12301 ggataagaaa gaaccaactt ctacatcact gaccgtatgt gcttcaatag aagcttgaac
    12361 ggtttcgtcg attttttcga tccattcttg tcgggaatgc gtttcttttg tttcaaaatc
    12421 tgcatcccaa tattgctgaa tgtctatttt tccatcttta tacgtataat aatggccttc
    12481 aggcaaacga tagacatctt tgaagaatgt ttcgccgttt aatggagaat attggaaagt
    12541 catataaggt ttcaacgctt ctttgttcaa ttctttgtta aaatcaggat gaggcagaaa
    12601 acttttgatt tctgaaccaa acataaatgt tccgttcatt tctgcataat aatatggttt
    12661 gatcccaaag tggtctcttg caccgaataa ctcattcttt tcattatccc aaatagcaaa
    12721 agcaaacatt ccgcggatct tttgcaataa ttctgttccc cattcttcgt aaccatgcaa
    12781 aaggacttct gtatctgcat gtgtttgaaa aacgtggcca gctgcaatca attcttctct
    12841 caaaggctgg taattataga tttcgccatt aaaaataatg atctttgtcc gatcttcatt
    12901 gtaaataggc tgagtaccgc cctctaagtc aataatactt aatcttctga atcctaaagc
    12961 aacatgttta tcgatatatt ctcctgaact attaggtcca cggtgaacga ttcgatccat
    13021 cattgtatta ataattgttt ttttgttgtc cttgtcgtta acaaaaccta caataccgca
    13081 catttaactt tcacatccta atataaaatt actgtttatt tcattcatat gcatcttatc
    13141 atagaataaa tagcggacga atattttctc tcttacaatt tcgtcacgtg aaaacaaaag
    13201 atgaaagttt ctgtttttac agaaaataaa gtcccaatat ctaatttacc atgttttctt
    13261 ttttgaggct accttattct cttcgtgtta aaaaaatatt agtggatgct atttccaaaa
    13321 aaaccagcaa aaaaagacac gacctaaaga tcgtgt
//
LOCUS       DAZULO010000071        13251 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000071 DAZULO010000000
VERSION     DAZULO010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.072"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1227)
                     /locus_tag="ACG8OA_002059"
     CDS             complement(<1..1227)
                     /locus_tag="ACG8OA_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFS4976822.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDIL"
     gene            complement(1224..3413)
                     /locus_tag="ACG8OA_002060"
     CDS             complement(1224..3413)
                     /locus_tag="ACG8OA_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4976823.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            complement(3547..4737)
                     /locus_tag="ACG8OA_002061"
     CDS             complement(3547..4737)
                     /locus_tag="ACG8OA_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HFS4976824.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            complement(5609..7240)
                     /locus_tag="ACG8OA_002062"
     CDS             complement(5609..7240)
                     /locus_tag="ACG8OA_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFS4976825.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(7254..8927)
                     /locus_tag="ACG8OA_002063"
     CDS             complement(7254..8927)
                     /locus_tag="ACG8OA_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFS4976826.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(8940..9257)
                     /locus_tag="ACG8OA_002064"
     CDS             complement(8940..9257)
                     /locus_tag="ACG8OA_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4976827.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(9282..10682)
                     /gene="lacG"
                     /locus_tag="ACG8OA_002065"
     CDS             complement(9282..10682)
                     /gene="lacG"
                     /locus_tag="ACG8OA_002065"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFS4976828.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            10919..11704
                     /locus_tag="ACG8OA_002066"
     CDS             10919..11704
                     /locus_tag="ACG8OA_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4976829.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            11707..12399
                     /locus_tag="ACG8OA_002067"
     CDS             11707..12399
                     /locus_tag="ACG8OA_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4976830.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(12673..13044)
                     /locus_tag="ACG8OA_002068"
     CDS             complement(12673..13044)
                     /locus_tag="ACG8OA_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFS4976831.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         4071 a         2709 c         2177 g         4294 t
ORIGIN      
        1 taaaatatcg tttgcaatta gctttcttct tcgaaccttt tcacctgatt cagtaaagta
       61 caaaattgct aacttgaaaa ataaactatg attatttacc ttaattggtg atatatttat
      121 agcatcaaaa ttgcaatacg gtaattcttt ctttaaatac tcacccagtg aaaacagagg
      181 atagtgttga ggagcaagta cacaaatatc tttttcatga atgccgcttt caatcttttc
      241 ctttattata ttttttatta tgttgggtaa atcctcctta gtaactttgc aatcataact
      301 tataaaacca cggtcgtttt tattattccc tctgctttca atctcgtaag gacaactctg
      361 aaaattagag tagaagtcta ctaatctctg attagacctg taacagccat gtagcgtctt
      421 tgattgaaaa gtgtgactag ttagcatttc tagttcttca actgatttag ctataccatc
      481 taatgatcca taaattgctt ggtcaatatc tccaacaaac attgactgaa tttgagggtt
      541 gttttgaagt aatttagcta ttacattgta ttgtaattct ctagtatctt gaaattcatc
      601 cacataaatc gcccttatat tactagcaat attacgtgca acgttactgt ttacagttaa
      661 aattttatac gctactagta aaatcatatc aaaatcaatt tctttatttt tttccaacat
      721 agtgtgatac atttcagaaa cttgagtata ctgaacttca ttaattttaa aatcatgaga
      781 aagtgacaaa tcaattttgt tcagttcata cgttttgaga tttaaattca gcatattatt
      841 aatttggctg acgtaagctc tttgagtata atcatcaata attgtaaatc cttttgatac
      901 tcttgagaga ttcattttaa aaggataaat aataaattca agacaaaact gatgtattgt
      961 ccccacccaa atattaatat catcatctat actaagtaaa cttaggcgtt ccttaatttc
     1021 ttcagcagct ctattagtgt atgtgatagc aattattctt tttagtgatc catcgtgttt
     1081 aattgcttgg taagcaattt tccttgtaag tacccttgtt ttcccactcc caggacatgc
     1141 atttacataa aggttatctt cactttccac aacatctttt tgttctaaat ttaaatcatt
     1201 tacgattttt tcgaagatat ttgtcatttt gaaacctcag cataaagtat ttttgtgtaa
     1261 ttttctatct gattgattag atacacaaca tcctcatctt taccaaagta ctctaataaa
     1321 gaaattattg ctatattcaa atcagctgta tcacttgttt ttaaactagc tgataaagct
     1381 gacaattctg tagtttcctc gtacaagtcg attgtatgga gtagaatttt tttataaatt
     1441 gagagatcta ctatttcctg acatgcaaaa gcaatagcat taagaatata cttaggaatt
     1501 ttgattaaaa agtcaatttt ttcacctaac tcaattgcaa accacccctt accaacatga
     1561 ttggctagtt ttaacatcgc taaatttctt tctaaatatt gttcactttg tatttcttct
     1621 ttataagcag ttattgtttt tttatctttg aatgcgtcat ctataattgg actgacataa
     1681 gttagaatat tttctttatt cacatctaaa aactcaattt caaatgttgt attagcataa
     1741 aaacaattaa cccaattgtt ttcattaaaa agggaggata atttttcttt tcttgcttga
     1801 cctttctctt gtgcagtgct tttatacaat cgatgttctg atgggattgc ctgttcatct
     1861 aaatcagtta caatagcaca ctttctctgt attctatcat cgtcaaacaa gctagcgata
     1921 tattcaaacg aagtactacc aacattaatc aaacctattc caagttcgtc taagtttata
     1981 ccaaataatt tttttactat ttgaggaata agtatttctt cagcatctcc ttcaactaag
     2041 ataacacctt tagaaaaaag caatccattt cttttaacat ctaaatatct ctctactgcg
     2101 tttattagat ttagattgtt cttttttaac ttttctttag caaatgagtt cagattattt
     2161 gctggattca taacgataga tttgccatta aacgatttta atacattcat ccgagatatt
     2221 tcagacactt cagctaaatg tacagaatgt gtggtcatga gaatctgagt atagtttttt
     2281 tgaagattta atccttcgaa cagagttttt tgaatatgat gatgaatatg agcttctggt
     2341 tcttcaatca acataatatt aagtagttct cttgaacgac acgcttcaaa ttcgactatt
     2401 ttcaaagcta aataaatcat atttagatgt cctaacccta gtaagtctaa gtcgatattt
     2461 tgttctggct tcattgaaag aaattttgct aaagaaccca tatcatctga catagttgag
     2521 gaaagaagaa gattaggaga atacactgaa ccaattatgc tatttagttg ccgattaata
     2581 ttttcgccaa ttttcctaat ttcaggaaca ctggtaatag tctcatttaa ttgctgtata
     2641 attgttttta atgactcaac attatctgat gatatttttg attcaattgt ttccatgatt
     2701 cttttgactg ggtttcgatt gtttttcatt tctctaagaa catctcgaag agcgtcaaca
     2761 taaacaaccg aaatatgctt gaaaatatct gacatgtcaa ttcttgagcc caacagagct
     2821 tcatcgtctt cttcaggatt actagctata ttgtcattga gtcttcctac caatttatca
     2881 taattttcat tgatacagaa atttagattt gatttagatg tgtagtaaaa ttcatagtcg
     2941 gatagacgaa tagaatttct aaattcgtta aattgatgag agtctaattc agtggaaata
     3001 tcaaataatt gctttctaat agctttgtta ggtctaataa atagagaaat actacctaca
     3061 cctttatctg catttgaaat caattcttct aagattgttt gactttcaga atttaaagaa
     3121 atggaagcac aaatctcatt gtcaaattct tcttcgctga ttccttcaaa tgtcgcagag
     3181 ataataatcc aatgcccttg ccacccattt tgaattccat gaaaaaaatc tgattctttt
     3241 agtgttttac tactatagta ataactatca tctaataaga ttctcaatgc agtaagagca
     3301 ttggttttcc ctgaatcatt ctcacctata atagtatttg tctcattatc gaatcgaaag
     3361 atttcggatt cataatttct aaaatttcga atttgaagag tagatataaa cataagcaga
     3421 tctcctaact atttaattaa tatgattata tagtaagttt atcataaatt atgttagtaa
     3481 aagttctcat tataagggag cgtcaataat tttgtgtaaa tgattcttcc ctactgcaga
     3541 ttgtttctaa ttctcaatca gttggcttag catttgttcg agctcgcctt cggcctgttg
     3601 gaaaccttta tgactgcgac ctaggaattt ttggttgtaa gtatcaaaag acgagactaa
     3661 gaaacgttcc atcgactctt cattttggaa ctgttccttg cggtggctgt atttcttgat
     3721 ttgtttatta aaggattcaa tcaagttcgt agaataaata ctcttgcgta tggccaaggg
     3781 gaaatcatag aaagtgagca agtgatcgtt cgaaagaatc gattcaacca cttttggata
     3841 gctggttttc catttttccg caaaagtacc acgtgcttcc aacgccacct ctttagatga
     3901 ggcttgatac accattttaa aatcatcaca gacttcctta cgatcatcga cacgcacttt
     3961 atgactgata ttacgggaaa cgtgtacaca acaatgttga aaacgagctt tgggatagaa
     4021 ccgactaatc gctcctacca tcccctttaa gccatccgtg atgaacagga ggacattttt
     4081 caaaccgcgc tcttgaaggt ccaataaaat ttcctcccaa atcgtgatgg attcagtcgg
     4141 tgcaatcgca tagctcaata cttcctttga tccgtccggg cgaatgccaa ctgcgatatg
     4201 aatagcttcc ttggcgacgg ttttccgctt taacggaata tacgttgcgt ccatataaat
     4261 agcagcataa cggtcatgaa gctcccgccc attaaacgcc gttacctctt ctgtaaatga
     4321 ttttgttata ttggacatgg tttggggcgt gtagtaatgc ccatacattt tctcaatcaa
     4381 gtctgcgatt tccgacatgg taataccttt tcggaagagg tgaatgacgg tctcctctaa
     4441 cgtgtcattc gtccgtctat aagcaggaac agtctgttgc ttgaactcac cattgcggtc
     4501 gcgcggaatc tggagatgaa gttccccata ctcggtcttg accgtacggt cataggagcc
     4561 gttacgagaa ttacccgtgt taaaaccaat gcgatcatac ttttcgtaat ctaggaaagc
     4621 cgttaactcc gttttgagaa gcgtattgac tgctttctcc aaatggacac ggaataattc
     4681 attcaaatcg cctttgttcg ctagagtctt tagaatttct gtagtaaaat cgttcatagg
     4741 gaagtcctct cttctgtgaa tgtgttgtgg taactctatt ctacagaagg gacttccttt
     4801 tttctattta cacaaaatat tttacactct ccattataac aattgggtta aaaagaaaat
     4861 agatagtatt ctacctcata tttatttgaa agtaatatat aaaagacgtt caaattcgaa
     4921 cgcctctcgt taaccaaata ttggattcag aatctagttt tcatatataa acagagtatg
     4981 attaggtgca aaaattgcac ttttattgca cttttatttt tatttatagt gttttactgt
     5041 tctgtatatt gttttaactc ccttgatagt atcgcattct gttttataca ctttacgttg
     5101 aaaacagagt gggaataaag agccaacata atatggaggg ggaatcccta taaatcaaca
     5161 ttcttcaata gctaaaatgc ttgattttca ttaaaaataa gatggttccc taccaaaaga
     5221 gactataata taagttaatt tgtttcattc ttatcataaa aagaagttct cgaaaaacga
     5281 actgttttcc gagaactttt ttctatccat ctaagtgctc aaatcatatt cacgacggaa
     5341 ttctaaggtt accctttctt aaacaaactt tatgttatct gcacaacttt ttgcaatttt
     5401 ttcattgctt cttacgaaat cgttgaactc tctttagcat aatacaaagt gtagatgaag
     5461 tctggtgctt tactcgtttt ggtaaaaatg actggaaatc gtttctggtt atttttggca
     5521 aaggcgaggt gcaggttcga ttcctgcctt ctacgctaac gatgtggctg aagggaacct
     5581 ccgatcttga atcgaaacga tccgcagtct attgtttcac caatgaaaca aaatcaacgt
     5641 tcgtccaagc atttcctgaa gccttgctat aaaccccaat ttcacaaaca cctgttgtaa
     5701 ttggaatgtc tttgattgta atttttgtcc attcacttgt tttaacaacc gcagtattta
     5761 gttccgctcc accataattt ttgatatata actgattagt atcaaactct cccccagaca
     5821 ttacataggc actcaatgtg taagttcctt tgcttatgcc actaacattt tgataagctg
     5881 atgcctcgta tgcttgatcc tgccaaaaag ttagtttgta gtccccaaaa aaagtgcccg
     5941 cttccgtttt gatcgtaccc ttagcggttc cttgttgaaa ccaggtttgc caattggaag
     6001 gactggtcgt tggtccatcg ctttcgaaac ttgcattttt aactagattt atttgcgccc
     6061 ctgcttgttt aaacgcacgc aagcttggca aggcgtttcc agcaaaatca aacatcgctt
     6121 ggttgtccca ttcgtttccc atagtggatt gaacactgag ataattcata cctgcttgtg
     6181 ttgcccaact cacatttccg ttgtaccaaa gaggttccca atagaagaag cccgccccgc
     6241 gattattcgg tactgcctga atccgatcgg caagatccag caaatactct gcttgtcctg
     6301 tcggtgttgg ttcatatccc cccgcagctg cttcggcttc accaaaagca tttggcatag
     6361 tatcggcatt cgctgttgta tgcccataag ctgtttcaac tacgatcact tctttaccat
     6421 accgttggct gatcgcattc atattggttt gaagttcatt cattgaaccg tgccaatacg
     6481 gataatacga cactccaatt aaatcaaatg acacacctcg actcgtgatt tcatcaaacc
     6541 accaagtaaa tgctcctgta tcgccaccat cagctaagtg gagcattact ggagttgttt
     6601 taggttgagt gtctcgaaca gcttggatac cagacttcaa aaaagcagcc aatcgatcaa
     6661 attctccccc gccttctccc caagacttgc cataaggcca gagcattccg ctattcagtt
     6721 cattgccgat ctgaaccata tcaggataaa cgtccagctg cttcatttcc gacagaacgt
     6781 tctttgtata gtcgtacaat gccgtattca tttcttcaaa ggtcagacct tgccatgctt
     6841 taggaagatt ctgtttccca ggatcaaccc aaaaatcact gtagtgaaaa tccagcagca
     6901 ccttaagtcc cagatttttt gctcgttttg caaggtcaat cgtcgtttcc agatcatttg
     6961 tccctgctcc ataactttct ccattactat tataaggatc ctgccacaaa cgcagtcgaa
     7021 catagttgac accattattt tttaaaatct ctaaggcatc ttttgaaata tcgtcttcat
     7081 aatattttgc accagatttt tccatatctg acaggatcga aatatctgct ccgcgaataa
     7141 attcgctggc agatgcttca acagtttttg aaacagcccc caaagcccct aacacagctg
     7201 caaataaaac agctttacgc aaccaaattc ccattttcat tatggctcct cctttattga
     7261 aattggttca gaataaactg aacagctttt tcaggatctc tcgttaatcc gatatattct
     7321 gctccttttg tcgcaaccag cttgattcct aagcgatcgg tgtcttcttt gatatcatca
     7381 tagtaagaat tgacttgcgg tgctaagacc accatatcaa aatcctgcag catttcgtaa
     7441 tgagaaccat aagcacccgc cattgcactg atcggtgctt tcgattcaga tgcacctttt
     7501 tttaaagcat tagctaacat cgcacttgtc cctgcgccag cacaaagtac caacacattg
     7561 atttctttat ttgcatcgct gataacttga ctcgctgcca caggtacttc ctcacgtaca
     7621 tcggcagaag caccttcctc ggttgcgatc acctctgctt tttgtgcttc tttctctact
     7681 aagacagcat cgtatgcttt gcaaaatggt agatagatca ggaagtcaac gacgattaga
     7741 accgctgcta gaacaaaagc taagataccc atgcccgttc ctagaaccaa accgatcggt
     7801 gctggagttg cccaaggcaa aacgtacata aagctgttca tgcccaatac atcgacaaaa
     7861 aacttgaaca ggcaaacgtt tacaacaggt gttaacaaaa atggaataaa aaaataaggg
     7921 ttcaaaatca tgggtgccgc aaatagcaat ggttcgttga cagcgaaact tactggaata
     7981 aaggaagctt tcccaactgc cttcaactgc ttagacttag caaagagtag gaagataaaa
     8041 ggaacaacca atgttgcccc agttcctccc atagttccta caaaattccc taaacccacc
     8101 gtcagtactt tagaagcttg ttcaccggaa ttgaacaatt gcagattggt ttccacattg
     8161 gcatagataa ttgccgcaat tgccggctcc acgatcgacg gtccatgaac accgacaaac
     8221 caaaacagcg ccatcgctcc ccagataata gcgattccta agtacccgtc agctgctgta
     8281 aatagcggct gcagcaccgt aatgattgct tctgcaaatg agatttggaa gatttgtcga
     8341 atgcctaaat caattaaaac ggccgcaaat actgaaaaag agaaaggaaa aatatctcgg
     8401 aaggtttgcg aaatcgttcc aggaacttct ttaggcattt taatggtaat atctttcagc
     8461 acacaaaact tatataaatt aactgttaaa aatgcagcga caaaggaagc cagtagtccc
     8521 tttgtcccca tatattccgt tgaaaatcca ccttcaatcg gagagacgct taacaacagg
     8581 aaacttacca cagaggccag catcacagag gtggtgttga ttactttgtt tttcggtaag
     8641 cgacgattca tcgaatcagc caagcagcga gcagtggttg ctgaaacgag taaccctaca
     8701 acacccatcg aataattata gactttccat agccatgtgg agacagattc aggccattgg
     8761 taaccaaaaa tctcaggaac cgcagcaatc aaaatgaaaa tgcttgaaaa taaaatagct
     8821 ggcatcgctg ttaaaaatcc gtcacgaacg gctcctaaat aactattacg tgagattttt
     8881 tcaaaaaatg gtttcccttt ttcgatttgt ttaatgattc catccatagc gttcttctcc
     8941 tatccttgat tctgtctgta cagtacaata aagtcttgaa tcagatctct cagcaataaa
     9001 gtggtcatta aatgatcctg cccatggata aaaatgaatc ccatctccat ctctttccca
     9061 gatgcttctt ccgctaaaat ttttgtttgc gaattatgtg caagggttaa attttcgtcg
     9121 gcttctttca acgccgaatc aacatgttca aagtttcctt gtcttacttc tttcaataat
     9181 ttcaataatg tactgcgagc gtcgcctgaa tatgcaacga tctcaaaacc aagcatttgt
     9241 aattcctctt tattcatctt ttcttcctcc attatcactt attaaataat cgttttggtt
     9301 tcactgacca acttgtacca gtaagctgat tcttttgggt aacgcttctg ggtttcaaaa
     9361 tcaacataaa acaagccata ccttttgtta tacccatttg tccatgagaa aagatccata
     9421 agcgaccaca aaaaataccc cttcacattt accccggctg ttatcgcctt gcttaaggat
     9481 tccaaataga ctctcaaata gtcgattctc ggctgatcca taataatgcc atcttcaaac
     9541 tgatctttat accccatccc gttctcagtg atatagattt tgttgtaatg tgggtaatcg
     9601 cttttaattc gcagcaacaa gtcatataga ccttctggat aaatcagcca gtcccaatca
     9661 gttcgaggaa ttccttcttt ataaatacgt tctccgattc ccttcacttt ataaacagag
     9721 gtcccttttt cacccgtacc gttatgatgg attgcatttt ctccatcgta agctttgaca
     9781 aaatggcatt ggtagtgatt gatccctaag tagtcattac gcgtggatgc ttttttcaat
     9841 tcaacgaaat cttcttcagg aaaatgatag ctggcctgat ttgcttcaca tatctcatca
     9901 agagcagtca aggtttcagt tgaataatag cctaaatagg tagcatctaa taagaaacgg
     9961 atcgacaatg catcgtctaa aaaggcagca tgcttatctt ccggcgcgtc tgttgccgca
    10021 tatttcgtct ctaatgagtg aactacacca atttcacccg gaagttcgtt ttctttgaaa
    10081 tagttcacta cacgggcatg agcaaccatc atattatgaa gacaggcgac gatctttgta
    10141 aaatcatatt tgattcctgg tgggaaaaca cctaacaaat attgattggt tgcaactgga
    10201 tagatttcat taaaggtact ccacaccttg acttctttga attcatgaaa acaaaaaatg
    10261 gcataggaaa caaatgcttc tattgtttct cgattcaaaa aatctccatg gtcaaacaaa
    10321 cgtttagggg tatcaaaatg atgcaacgtc acaaacggcg ttacgtttct tttcttacat
    10381 tcttcgaaca cacgatgata aaaagcgatt ccttctggat ttggtttccc agctccatca
    10441 ggaaaaatgc ggctccaagc aatggataat cttagaccat ttacaccaaa acgttcacac
    10501 aattcaatgt cctttgcata ttgatgataa aaatcacttg ccggatcggg actgaatcgc
    10561 ccttgttcct ctaaaaagtc atcccaagca acagcgcctt tgccaccttc cttagtggct
    10621 ccttcaacct gataggctgc tgtggctccg ccaaaaataa aatcttctgg taatttaagc
    10681 atgtacatct ctcctcaaat ctataatcag ttttcgctct ttctaactga ttgcattgta
    10741 aacgcatcca aaacatttgt caacaaaaac aaacacaaat aatcataaaa accagaaaac
    10801 agcaataaaa tctattgtaa caaacataaa aaccacccta aatgtttact tttgttcgaa
    10861 ttagattgac tgttgtccgt ttatatgata gattatcctt aaattagagg tgaagacaat
    10921 gttgaaaaaa gaaagattgc ttactatcgt agaaatggtc aataaaaaag gaatccttac
    10981 cgttaatgaa atcatcaatc aattagatgt ttctgatatg accgttcgca gagatttaga
    11041 tgaattagaa aaatcgggca agctgctgcg tgtccatgga ggggctcaaa gtctttccta
    11101 cactttagat caagaacttt ctcacactga aaaagccact ctgcagattc aagaaaaaaa
    11161 agaaattgct gccacagcag cctccttgat tcacgaggga gaaacgattt tcttaggacc
    11221 aggtacaaca atcgaactat tggcgacgca tttactgaac aagaaaattc ggatcattac
    11281 aaataattat ccagtttttg atattcttgc tcaagcagat ggaccagaaa ttctactgat
    11341 tggcggtgat ttccggaaaa ataccggcgc atttgtcgga ccgatcacca acgacaattt
    11401 aagaagacta aagtttacaa aagcatttat cagttcaaat ggcgttcaca acgaagcgat
    11461 ctctacttac agtaccgaag aaggagaagc acagcagatc gctctaaata attcccgtaa
    11521 gaaatatcta ctcgttgata ataaaaaatt caatcgagat gatttttatg tgttttataa
    11581 tttgcatgat ttcgatctgc tcattacgga tcgccagatc tcgcgggagg ttcaagagca
    11641 ctatgcacaa tatgtcgatc tgcaaattgc tgattcaacc gaaaatacca ctataggagc
    11701 gtaactatga aagggattat atttgatttc aacggaacaa tgtttttgga ctctcatctg
    11761 catgaagctg cttggctgca tatgatacat gatcatacaa aagatggttt atcagacgag
    11821 gatattttaa aaaatattca tggtcggacg aatacggaga ttttgaaaca tttcatttca
    11881 gagcatttga ccgcctctga gatcgccgta ttgtcagaag aaaaagaaag ctattatcgt
    11941 atgctttgct tgcaaaatga aaacgagctt cagttgacaa aagggcttga agcgactctt
    12001 gaccagctga ctgaccaaca gattccatta acgattgcca cggctactgt caaagagaat
    12061 gttgcttttt attttgatat tttcgattta gcccgctggt ttgactggga caagattgtc
    12121 tatgacgacg gctctttccc tgggaaaccg caaccggata tttttcttaa agcggcagac
    12181 aaactgagct tagctccggc tgattgtctt gttatcgaag atgcttactc aggattactt
    12241 gctgccaaac gtgcggggat cggaacaatt attgcaattg atccattcgg taaaaacaaa
    12301 acagtatttg aacaggcaca gctaaataaa gatggaacca tcaaagattt tactggtttt
    12361 tggacagagc acctgtctgc cacatccgtt aattcttaaa cgattcaact aactaccaaa
    12421 gaaaagcaac tgaaatgcac acctcctttt ataaaaaact gccagtgaaa cgacatgaaa
    12481 gatacactgc tatatcctta gtgaaatata gcagtgtatc tttgactttt tcattctttt
    12541 aaccatatga cgcgttcatc tctatctata attaacatta aaaatagtgt tttcacatta
    12601 tttctcaaac caatcttaaa aatcatcagg agtacttttt tgatatactt ttattaatgc
    12661 tgggcgtaat ggttaaaaga tttccactag ttcctctcca ccaagctgag ttgaaaaatg
    12721 cttatctgta aacaacatag acaaagagga aataaacgtt tccgtatctt tctctgtccc
    12781 aaggatatta attaattttc ctactaggta agtatcatta aacttaagat ctgccttcac
    12841 atcaaaagtt ttgataaaag cttttatcat gcgtacaatt ttgtctttat cgccataatt
    12901 gatttcacca ctcatatcaa tatagtcact aatccctgaa gaaataatgg caactgcttc
    12961 ttctttcgcc ttatcagata attcatttaa tgactgggat tgattgatga cttcttgagt
    13021 tttatctgtt acgtaatctc tcattatcct atcggcatac tccgcaaagt aatcttgaaa
    13081 aaattcttct acagggtaac caacttcgtt ttcccaacca ggagaaattt cattatcagt
    13141 acttattaaa tctattaaaa accaacccgt tctctgagtg ttgattctat aatattttta
    13201 atttcctata taaatcaaaa atattataga atcaataagc taattaagaa a
//
LOCUS       DAZULO010000072        13169 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000072 DAZULO010000000
VERSION     DAZULO010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.074"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            58..1797
                     /gene="yfcC"
                     /locus_tag="ACG8OA_002069"
     CDS             58..1797
                     /gene="yfcC"
                     /locus_tag="ACG8OA_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFS4976832.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1954..3138
                     /gene="iadA"
                     /locus_tag="ACG8OA_002070"
     CDS             1954..3138
                     /gene="iadA"
                     /locus_tag="ACG8OA_002070"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFS4976833.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3249..3452
                     /locus_tag="ACG8OA_002071"
     CDS             3249..3452
                     /locus_tag="ACG8OA_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS4976834.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3553..4215)
                     /locus_tag="ACG8OA_002072"
     CDS             complement(3553..4215)
                     /locus_tag="ACG8OA_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4976835.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4228..5730)
                     /locus_tag="ACG8OA_002073"
     CDS             complement(4228..5730)
                     /locus_tag="ACG8OA_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4976836.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6278..7957
                     /locus_tag="ACG8OA_002074"
     CDS             6278..7957
                     /locus_tag="ACG8OA_002074"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS4976837.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8084..8563
                     /locus_tag="ACG8OA_002075"
     CDS             8084..8563
                     /locus_tag="ACG8OA_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFS4976838.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8782..9462
                     /locus_tag="ACG8OA_002076"
     CDS             8782..9462
                     /locus_tag="ACG8OA_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS4976839.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9470..10801
                     /locus_tag="ACG8OA_002077"
     CDS             9470..10801
                     /locus_tag="ACG8OA_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFS4976840.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10816..11778
                     /locus_tag="ACG8OA_002078"
     CDS             10816..11778
                     /locus_tag="ACG8OA_002078"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFS4976841.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11889..12227
                     /locus_tag="ACG8OA_002079"
     CDS             11889..12227
                     /locus_tag="ACG8OA_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFS4976842.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12306..13013
                     /locus_tag="ACG8OA_002080"
     CDS             12306..13013
                     /locus_tag="ACG8OA_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFS4976843.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4180 a         2330 c         2896 g         3763 t
ORIGIN      
        1 ttgattatcc attttttatt catcaaaatt actgaaaaaa gaaaggaaga aataaatttg
       61 aagaaaacaa attcttcagg aaaaccgaaa cgcgacttca ctaaattatc gacaccccat
      121 acgtatgtca ttatttttgg tgtagtcatc tttgcatgga tactgacttt tgtcgttcca
      181 gcaggaaagt tcagcacgca agatattgaa tacaaagatg caaatggcga aacaagtact
      241 cgtactgttt tacgtcaaga ttccttccgt tatgcttacg agctggataa gtcgtatgtg
      301 tttgatcaat tagaagaact gcaagatcat ccagcagaac gtgaaaaact ggatgttccg
      361 gaaaaaggat tggaaaaagt cattgctgac ggagaaaaaa atcttacgca ggaaaaacta
      421 gatgaaatct ccttgacaga tgatgtgctt tacgatcagt acggcgaaaa tatctatgat
      481 acttctaaaa aattgcataa aacagccaaa atatggggaa cagatgattt tggcggcttt
      541 ggtttcttaa attttgtttt tgaaggacta gtatctggag ataagtatgg atctgctgta
      601 gggatcgctg cattgatcct tgttgttgga ggtgcttttg gtattattat gcgtaccgga
      661 gcgatcgacg caggtatcta tgcgtttatc agtaaaacta agggactgga gcgtttagct
      721 ttaccgctat tattctttgc tttttcattc ggtggcgcta cattcgggat ggcagaagag
      781 gtaattccat tctcaatggt gatggttcca tttgtcattg cgttaggata tgactcgatc
      841 gtagcagtaa ccgtgactta tgtcgcatct caagtcggaa atgcgacctc ttggatgagc
      901 ccgtttagcg tagcggtggc tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca
      961 ttccgattga ttatgtgggt agttgttact gcattagccg ctggttatat gatgatatat
     1021 gcagaaaaaa tccgtaaaaa accagaaagt tcgttgactt acaaatcaga tgattatttc
     1081 cgttctcata tcaaaaagac atctgatgaa aataaaaaat tcatgcttgg gcataaattg
     1141 atcttattag agatgttagt tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc
     1201 tattacattc cagaaatcgc ttctcaattc tttgtgatgg gattagttgc tggaatcatt
     1261 gctgttcttt tcaaattaga tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct
     1321 ggtgcagctg atttagctgg tacagcaatc gtagtaggta tggccaaagg gattcttctt
     1381 gtcttgggag gatctgatgc atctgttcct tcagctttga atacgatttt gcatgggatc
     1441 gggacggcgt tgaccggtgt tccagctgtc attggtgctt gggcgatgta catcttccaa
     1501 agtttgttca acttggttgt tacatcaaat tcaggacaag cagctttgac tatgccaatc
     1561 atggctccac tggcagattt agtaggtgta tccagacaag tagcagttct tgcttatcag
     1621 cttggttcag gttttatgga cgcttttaca cccgtatcag ccagcttgat tggtgtgcta
     1681 ggtgttgcac ggatcgaatg ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc
     1741 ctgtttgttt taggaactat tttcatcatg attgccattg caataggtct gcagtagtac
     1801 tggagacctg taaataaaaa agtaatccaa acagtagata aggactccaa gaaccgtcat
     1861 tttacggtaa aatcaaagga gtaaagatcc tatcgtttgg attatttttt aagatcctat
     1921 catcagttat acttaaagaa agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg
     1981 tttgcaccag attacttagg aaaaatggat gtactgattg ccggtgggaa gattttggct
     2041 gtagaagaac agctagaagg cggctacgaa ggagtggaag tagaagaact ttcaggtgag
     2101 ggaaaagtac taaccccggg tttcattgac tgtcatttcc atattttagg tggaggagga
     2161 gaaggcggtt atcaaaatcg aacgccagaa gtcacattga gccaattgac tacagctggt
     2221 gtaacgacag ttgtcggttg tttgggaaca gacggagaag gtagagatat gactgcgttg
     2281 atcagcaaag cgaaaggtct ggaagcagaa ggaatctcca cttatgtata cgaaggttcg
     2341 taccgattgc cggtaaaaac agtaacagat tctatcatca aagacttttt gacaattgat
     2401 aaaattatcg gaatcggaga aatcgcagta tccgatcacc gttcttctca gccaagtttt
     2461 gaagagttcg ctcgtgcagt agcagatgct cgtgtaggag gcatgttatc cggaaaagct
     2521 gggatcatca atgtccactt aggcggagga aaaagaaagc tagagctatt gacacgaatc
     2581 gtagaagaaa cagaaattcc gatcacacaa tttttgccta ctcatgcaaa tcgcacacca
     2641 gaattatttg aagcttgcgt ggcatttgcc aaacgtggtg gcacgattga cttcactgct
     2701 agcgaagatc ctgatttttg ggagaagaca gatggtgaag ttcgcttcag caaagcactg
     2761 aaacgattga tcgaggaaga tgtttcttta gataatttca cgatgacctc agatggacaa
     2821 ggaagcttgc cttattttga tgaaaacaat catttcctag ggttaggagt aggaagtgcc
     2881 aaggcattgc tagtagggat caaagaagct gtgcaaaaag aaagtatccc tttggaaatc
     2941 gccttacgag ctataaccag taatccagca cgtatattga aattggataa aaaaggaaaa
     3001 atcgaaattg gtgctgatgc agatctgtgt atcttggata aagaaacgct tgatattgat
     3061 acagttatcg ctaaaggaga aataatggtc caagaaaaag aagtcaaagt atgggggact
     3121 ttcgaaaaaa gtttttaatc aacagctcta actatctttt ttagaaaacg agcgttctgt
     3181 ttgtctgtat ctgttgttga tgttaccctg aaggtagatc aataaacaaa ctaagaggag
     3241 gaagcacaat ggcagattta aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg
     3301 aaggaaaaga agcatacggc aaagcaacag acgacaagag caaagaaaca gaagggaaag
     3361 cacaatcact aggtgctgac atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag
     3421 acaagttttc tcgaaaagac gaaaaaaaat aaaaaccact tattactaat tgaatcaagc
     3481 aaataaaaaa tccgaaccat atctgcgaga cagatatggt tcggattttt tatttgatca
     3541 ttatcctgtt ttttaaactt cgattttttg aaatttttta ttttttagtt caaagacttc
     3601 atcacagctt tttgctactt caatctcatg agtcacgatg ataatacagc gattttgttc
     3661 atgggcgatt tcttggaaaa gtttgacgat atcttgtgaa gttgtttcat ctagatttcc
     3721 agtaggctca tccgctacga ttagttgatg ttcacagcag atagcacgaa cgatagcgac
     3781 tcgctgctgc tgacctcccg aaagtttggt cacctgtttt tttgctaata tttcatcaat
     3841 tcctacacga gccagtcgat ctaaagcaac ttgtcgataa ttctcttgct tggtcttagt
     3901 gatttccatt gctgctatga cgttgtctag agccgataaa taaggaagca agttgtaagc
     3961 ttggaaaata atcgagacat catttttacg ataacttctt aacccgattt tgttcagtga
     4021 acgatcttga tagtatacct ttccttcttt tggtgcatct agtccagaaa taagcgaaag
     4081 aaaagtcgtt tttccagaac cacttgctcc taaaatagcg tacatttttc cagcttcgaa
     4141 attcagttct acatctttat aaagcgcatc ttgcggatct tgataccaaa atcccaattg
     4201 ttctgtccgt aacatcaaat tcctccttta gtttaatagg atttttcttg ggtttaatct
     4261 taatatccct atagaagcta aaaagacaga aaacagactg attccaaatg ctactccggc
     4321 taatagtccc aattgtgcag gtttgacatt gatttccaat tcgctgactt gatcagaaac
     4381 agcaaatgga ttattcgtgg ttccttgttg accatcgcgt tgaccgcctt gttcttgtct
     4441 gttacctggc atttgtccac cgccaggagc ttgctggttt tgtgtcgtgg tttctgtctg
     4501 ttggtcaagt agctgctgtc ccacggcatt cgcgacgacg tttccgctaa gcgaagcaac
     4561 tcctaatgca agaatcatac aaatagccac ttcggtaaac aattggagaa tgattttact
     4621 tctggattct cctaaagata gcaagacgcc taactcatgc cgacgctccc gaatggtaat
     4681 cataatgatc agagtaagta tgataatacc ggctacagcc actagtaaaa caacattttt
     4741 agaaaaactt gccacgttgt ttaaaggttc caacattgac tgatacatgg aatcatttga
     4801 ttgtaagcta aatgtatctg tgtcgatcag cttctcagct tctttgagaa actcatccat
     4861 tttatctgga tcagatagag tgtaaatagc agaatctatc gtgttatcgt cagatgtccc
     4921 atttagttca tttgcaaaag tgtaagaagt gtaaagtgta ttcgacggat tcatgaagtt
     4981 gaatttcata cccatactgc cgcttgtttc gctggattca taaattcctt tgatcgtcat
     5041 ttcataagtg gtatcttcat cttcagtact cgtgattgtg aaagtatctc cgactgccag
     5101 gtcatttgtt tctgctagag tagaatcaat gactacattg tttgtgcctt tgtcgctgtc
     5161 atctattcct tcaccatccg tgatttttgc tgttccatca gaaaaattac tattttgtgc
     5221 agttgctgac acaccactga tttgaaagtc tgcttgagtc atttgcggat tcccgcccat
     5281 tcctccagca ggttcaccgc ctgcatcagt agtttctgaa ctgtcttcac ttgaatcaga
     5341 actagtgatg gcagttattc catcttttgc catagctgag gcggaacttt caaaggaata
     5401 actctttaca ttatctaatt cagctatttt ttccgcgtcc gttaaggaaa ctggtgtaag
     5461 agaaaaactg cctggatctg gacgttcttc agtggtagaa ccgcttgaag aagtttgttc
     5521 ctgtttttta aatgaagctt ctcgattggc acttaaagtc actgttgctc caacttcttt
     5581 ttgtgcttga ttcgctgctt cttctgcagc gcttcgaatc gtgaggccag ctagcacaaa
     5641 aataagaatg gctgtgaaca cggcaaacaa taacaatgaa cgtccccatt ttacttttgt
     5701 acttttccat gcacgtttca taaaattcat attttgcatc cctcctagta gatgcatttg
     5761 ataatagaca acctatctta aagctagctt aaatcttatg ctgtttgcac accatcaaca
     5821 tgaggttctg tatatataaa aagtcactat agttattgca aaaaatgtgg taaaatgaaa
     5881 aaagtatgtt tcaaaccgac gaagaagaaa tgaaagagga atagcaaaag aaatcctaga
     5941 tgctacagag ggatttttac gtgcatagtt gttctaacaa ttgctgatac tagcgattta
     6001 gtttatttgt tgtataataa tggggaacag ctgaatactt atataagtat tctggctata
     6061 gcgtttcgca aaagatccat tttgttaatg aagtatatcc aaaataaaat gcccatccaa
     6121 ctgtcagaac gcggatgtgc tggtaatttt cctgctgatt ctccataagt tgagaaatag
     6181 tgattttgca tatgcttcat tggaatggct catatattta gataaagaag aaaatcgaca
     6241 tctttaaact tggatgtcat aagaagaggt gaagtttgtg agtaccatta aaattatccc
     6301 attaggcggc gttcgtgaaa atggaaaaaa catgtatgtc gcagaagtgg gcgaggatat
     6361 ttttatttta gattgtgggc taaaatatcc tgaaaatgag ttgttgggga tcgacgtcgt
     6421 aatccctgat tttacgtatt tagaagaaaa cattgatcgt gtagctggtg tctttttaac
     6481 acatggacat gcagatgcga tcggggcact tccttacttg ttgtcaaaaa tttctgtacc
     6541 agtattcggg acagagttga cgatcgagtt agcaaaattg aatgtcagtc gtaatgaagc
     6601 agccaaagga tttaaggatt tccatgtggt agatgagcat acagaaatag actttggtga
     6661 aacagtagtg agctttttcc gtaccactca tacgatacca gattcagtgg ggatcaatct
     6721 gaaaacaaaa gaaggtagta tcgtttatac gggagatttt aaatttgacc aaacggctat
     6781 cccaatgtat caaacagatt atgcacgatt ggcggaaatc ggcaaagaag gagtcttagc
     6841 cttactaagt gaatcggcaa atgcagaaaa tcctcagcct gtagcttctg aagcccaaat
     6901 agcagatgaa gtatttgata cgatcaaata ttgggaaggg cgtatcatcg tcgcttgtgt
     6961 agcaagtaat ttgcaacgag tgcaacaagt attggatgca gcagaaaaag cacaacgaaa
     7021 agttgtcttg actggtcaag atttcgaacg aattattcgc acagcgatgc gtttagaaaa
     7081 acttcaatta ccaagtgaag atctgcttgt tacgttgaaa gacatgaaaa actatgatcc
     7141 agaagaactg atcattttag aaactggccg aatgggtgaa cccatcaagt ctctgcaaaa
     7201 aatggcaaac ggtacacacc gtaatgtcaa gattgaagaa ggagatttgg tttatatcac
     7261 tactacgcca agcatcgcga tggaaaccat cgtggcgaaa acggaagata tcatttatcg
     7321 agctggtgga acagtgaagt tgatctctga gaatatgcgc gtttctggcc atgccaatcc
     7381 taatgacttg caattaatga tcaatttgat gaaaccaaca tattttgtgc cagttcaagg
     7441 agagtatcgt gaattagctg ctcatgcaga tttggctcat gcagtaggga tgccatacaa
     7501 aaatatctat attactggac gcggagacgt gctggaatat aagaacaagc gtatgagtgt
     7561 tgcaggaact acaagtgcag aaaatgtaat gattgatgga ttaggcatag gtgatatcgg
     7621 aaacattgtt ttacgtgatc gaaaaatctt gtcagaagac gggattttcg tagcggttgt
     7681 cactatcagt cggcgagaaa agaaaatcat ttcaaaacct caaattactt cacggggatt
     7741 cgtttacgta aaagctagcc gtgatctgat gaaagaaagc agcaatatga ttgaaacaat
     7801 cgttgagaaa catttagaaa gcaatgattt tgagtggagc aaattaaagc aagacatccg
     7861 agatcaatta ggccgttact tatttgaaca aacaaaacgc cgcccagtga tcttgccagt
     7921 cattatggaa gctactcaac gtcgaaataa aaaataaaca aaaagagtaa gatgaacctc
     7981 tctatagagt gctcgtcttg ctcttttttt gcgaaaaaca aataattttt gaatacagca
     8041 tccctcgctt tataataaac gatagaataa agggagtgaa tcaatgatga gtcaaacaaa
     8101 agtcgaacgt tatttagaag aacaaaagat cagctatcaa ccacttgatt ttagtcatgt
     8161 ggtagaagaa tctttcacag aagaactaca aaaaagaggg attgatccag ctttgatatg
     8221 taaaactctc gtagtgaaag gaaacaaaac tggggtgatc atcgcattga ttcctttgaa
     8281 cgatcactta gattacaaaa aaacgagaca agtgacgaaa gatcgtaaag ttggttttcc
     8341 tggaatggat tttgtattaa tgcatacagg atatccccac ggagcaaaca cccctattgg
     8401 aataaaactc gcacatcctg attattgttt tttagcagac agttctttaa aagaaaaaga
     8461 agaagtaatt gtgtcaagcg gtgaaatcgg gagaagcctt cggataaaag tagccgattt
     8521 agaaaagatc gtccatccta tttacgctga tttaattagt taagggacga gaagagaagc
     8581 taagcagagc agagacctca aacttgagat tatatctgct tggtacttgg tgcatcctgt
     8641 attatctgtt acaattgctt tgtacatact gtactatcaa tccaatgaaa atatatttag
     8701 cagctatatt ttttcattga tgtgcaagtg atgaaatcgt ttttaataaa aaagatacag
     8761 agaaaacgcg aggtgtaaaa tatgaaaatt acgtatcatg gacattcctg tttaacaatt
     8821 gaaatggaaa atggccaacg cgtgatgatt gacccgttta ttacaggaaa tccattgaca
     8881 gacttaactg cagaagaagt aaagacagat tggttgctga ttactcatgg tcatagtgac
     8941 cacataggag atatgattcc tattgcaaaa gaaaatcaag ccacaattat cagtattgtt
     9001 gagattgctg attatgcgaa cagcagagga gtagattcat tcggtatgaa tatcggtgga
     9061 aaacatgcgt ttccattcgg cgtggtaaaa ttcgtacatg cacagcatag ttcaagctat
     9121 gaagtagacg gtatcgttca gtatatgggc gagcctagcg gaattattat tcaagcagaa
     9181 ggaaaaacga tttatcatgc aggggatact gcttatttca gtgatctagg attgcttgca
     9241 gaagaattcg atattgatgt cgctttcttg ccgattggcg ataactatac gatggggcca
     9301 gaagatgcag caaaggctgc gcaaacaatt cgtgccaaga aagtagttcc aatccattac
     9361 aatactttcc ctgtcattga acaagatccc gaatcttttg tagagttact ggaagatagt
     9421 gaaggaaaaa taatgagcgt ctcggaaacg ctggaagtgt aggcagaaaa tggcgacaaa
     9481 acatgatttg attttagaat atattgaaag tttacctgtt ggcaatcgaa tttctgtaag
     9541 aagcatcgca aaagaattga atgtcagtga aggaacagcg taccgtgcaa tcaaagatgc
     9601 agaaaatgtc ggactagttt caacaattca acgagtaggg accatccgta tcgaacgcaa
     9661 attaaaaaaa catattgaac gtttgacatt tggcgaggtc gtcaggatca ttgaagggga
     9721 cgttttaggc ggggcggcag gattagacaa ggtcttgaat aaattcgtca tcggcgcgat
     9781 gactaaacat gccatggaac gctatattac gccaggatca ctaatgatcg tcggcaaccg
     9841 ggcggaagtc caaaaattag cattggaaga tggcgccgca gtattgatca caggaggatt
     9901 caatacgact gctgagattg caaagctagc tgatgatctt gaaatgccga tcttacgcac
     9961 ctcttatgat acatttacag tcgcaaccat gatcaaccga gcgttaagcg accaattgat
    10021 caaaaaagat atcatgcttg tcagtgatat ctatatgcca gctgaaaaga ctcattattt
    10081 gcagcagaca gatacgattt ctgattatca gcgtttgtca gaagaaacgc agcattcgcg
    10141 ttttccagtc gtcaaccgcc atcatcgttt gatggggatt gttacggcaa aagatgtttt
    10201 agggaaatcg ccaaaccaga tcatcgatcg ggtgatgacg aaagaaccaa tcagtgtaaa
    10261 gaaaacaatg agtattgcct ctgttagcca tcaaatgatc tgggatgggc tagaagtcat
    10321 gccggtcgtt tccgatgact tgacattgga aggcttgatt acccggcaag atgtaatgaa
    10381 agcgatgcag cttgtccagc gacaaccgca gattgcagat acgatttcgg atcagatatc
    10441 tggaaatatc catacgattg atactgaccg tgaaggaaat cgtttggaaa accctaagtt
    10501 caaattcgta gtagcaccac aaatggtcaa tggcgttgga accatttctt ttggcgtact
    10561 tagcgaaatc atttctgatg tcgcacaaaa aacaatggtg atgaaccaaa aacgaaatat
    10621 cttgattgaa caagtgaacc tacattattt gcggctgatc caattggaaa gcgaattaga
    10681 cattcgtcca cgtgtattgg aaatcggcag acgttccgct aaactggata tcgaagtgtt
    10741 tattgaaaac accatcgttt ccaaagcaat cgttgtttgt caagtaatgg aacgttctta
    10801 gaaagcagga gtgaaatgac agtacagcaa gaaatagtag atacaataaa aaaatacgat
    10861 aaaatcatta tccacaggca tttgcgccct gatcctgatg cattaggatc acaagtagga
    10921 ttagccgaac tattacggga aagtttccca gaaaaagaaa tacgtcaagt aggtgaaacg
    10981 attgaagggc ttcgcttttt agcagaaatg caagaagttg aagatgtctt ctaccaagga
    11041 gcgctagtca tcgtgactga tacagctaat tcacccagaa tcagtgatca aagatatcag
    11101 ttggcagaaa aactgatcaa aatcgaccat catcccaatg atgaccctta tggagatctt
    11161 gtgtgggtaa atacaaaagc aagtagctgt agtgagatga ttgcagaatt tgctttgatg
    11221 tttcctgaat ctttaaagat gaacgttaag gctgcacggt tactttatgc aggaatcgtc
    11281 ggagatacag ggcgcttttt gtatccctcc acgacgtccc gtacattaga gattgcttcg
    11341 atattgagaa attatccatt tgatgcaagt gctttgaaca gagagattga acaaatgccg
    11401 atgaaagtcg ctaaactttc cggttatctt tatcaaaata tccaggtgga tgaaaatggt
    11461 gcaggaagaa taatccttcc gcaagaaatt cttgataaat atggaatcga tgattcagag
    11521 acagcttcga tcgtttcact tcctggagtg attgacgaag tgctggcttg ggggattttt
    11581 gttcagcagc cttcagggta ttaccgcgta cgtttacgtt ctaaggggcc gatcatcaat
    11641 gaattggcta aacgccatca tggaggaggc catccattag caagtggtgc gaatgcaaaa
    11701 gatttagcag aagtagagga aatctataaa gagatccaag atatatgcca atcgtataag
    11761 agcagaaaaa cagaatgata cttcgttcga aaacgtgcct tttgtagtaa aaatcacaaa
    11821 tgtatctttt caagatcttc tttttccgtt acaatagtgc tcactaaaga ttttaaagga
    11881 gaaagaagat gacattacca aattgcccag aatgtggctc agagtacaca tatgaagatc
    11941 gaggcttgtt gatttgccca gaatgcggga acgaatggaa caaagaagaa caagaaactg
    12001 tcatagaaga cggattagtg gtattagatg caaatggaaa ccgtctgtta gagggcgata
    12061 gcgtgacagt gattaaagac ttgaaagtaa aaggtgcgtc aggtgccatt aaacaaggaa
    12121 caaaagtcaa gaacatccgt ttagtagaag gcgatcataa tatcgattgc aaggtagaag
    12181 gatttggacc gatgaaactg aaatcggaat ttgtgaaaaa gaattaaaga atattcaagg
    12241 tcttttcaca ttcttttcct actactaatg ataaaataaa gtaaacgaaa aggagtgaaa
    12301 cgaaaatgta tggttatgga atgtatggtt ttttagatcc gacatttctt ttggtcattc
    12361 ttggattagt tatttccgga attgcgtcag cgtacgtcaa tagtactttc cgaaaatacg
    12421 atcaagtgag aagcaaaaac aatgtgactg gtacacaagc agcacaatat atattacaaa
    12481 gtcaacagat aaataatgtc ggtgttcaac aaattgccgg agatctcaca gataactaca
    12541 attcaggtaa taagatgttg agtttgtctc aagcaactgc acagtctact tcagttgctg
    12601 cgataggtgt agcagctcat gaatgcggac atgcggtaca agatgcttcg ggttatgtcc
    12661 cattgaagat tcgtgcagca cttgttccag cagctaactt tggttcgatg atttcgttcc
    12721 ccttgattat ggtaggtgta ttgcttagct ggaactctac gttaatcaat atcgggattt
    12781 tcgctttttc tttagcatta cttttccaat tggtgacatt accagtcgaa tttaacgcgt
    12841 ctcggcgagc aatccaaatt ttgagtgaag gcggactact gacggaagaa gaagtaccga
    12901 tggcaagaca cgtattattt gcagcagcac tgacttatgt tgcagcagcg ctttcgacat
    12961 tcctgcagct gttgcgcttg atcttattgt tcggcggcaa ccgaagagat taaaataaag
    13021 aagttgtgaa atcagcgctt tgatctgagc taataccgag agttagctga ttgaatacca
    13081 tttatttgga ataagggctc tttgtcaata aggactgatg ggttgtacaa aatcaaatct
    13141 gggtcagaat gaacctcatt ctggctcag
//
LOCUS       DAZULO010000073        13028 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000073 DAZULO010000000
VERSION     DAZULO010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.075"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1349
                     /locus_tag="ACG8OA_002081"
     CDS             <1..1349
                     /locus_tag="ACG8OA_002081"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFS4976844.1"
                     /translation="IVDILGYAIAPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYN
                     QFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQF
                     ASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHP
                     DNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMN
                     PPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVL
                     FRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASR
                     EFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFE
                     EEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1339..2556
                     /locus_tag="ACG8OA_002082"
     CDS             1339..2556
                     /locus_tag="ACG8OA_002082"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS4976845.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2569..5718
                     /locus_tag="ACG8OA_002083"
     CDS             2569..5718
                     /locus_tag="ACG8OA_002083"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFS4976846.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(7653..8318)
                     /locus_tag="ACG8OA_002084"
     CDS             complement(7653..8318)
                     /locus_tag="ACG8OA_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4976847.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            8444..10480
                     /locus_tag="ACG8OA_002085"
     CDS             8444..10480
                     /locus_tag="ACG8OA_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFS4976848.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            10484..11122
                     /locus_tag="ACG8OA_002086"
     CDS             10484..11122
                     /locus_tag="ACG8OA_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4976849.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            11150..11578
                     /locus_tag="ACG8OA_002087"
     CDS             11150..11578
                     /locus_tag="ACG8OA_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976850.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            12035..12946
                     /locus_tag="ACG8OA_002088"
     CDS             12035..12946
                     /locus_tag="ACG8OA_002088"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4976851.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         4524 a         2063 c         2530 g         3911 t
ORIGIN      
        1 caatcgttga tattttggga tatgcaattg caccagagta tcttttcaac gtcttagcgg
       61 atcaagcaaa acaagcaacg ttccaattga acgatctaaa caaagccttt gtgcaattgg
      121 catctaccta caatcaattt aatgggttat ttgatgatgt ggatctccaa tcaaaaaaac
      181 taggaacaga tgagcaacaa cgaaatgtga cgattaccga agtcatcaag aaattgaatg
      241 atgtagatgt tttagaacac gatggggacg tgatcgggga tgcgtatgag tttctgatca
      301 gtcaatttgc ttcagaagca gggaagaaag ccggagaatt ttacacacct catatggttt
      361 ctgatatgat ggcgcaaatc gtaacgctcg atcaaaagga acgacgattc tttagtgttt
      421 ttgatccaac gatgggttca ggttcattga tgttaaacgt acgtaactac ttaacacacc
      481 cagataacgt taaatatcat gggcaagaac taaatacaac gacatacaat ctagcaaaaa
      541 tgaacttgat tttacatggt gtcgacgccg aagaaatgaa tctccgcaat ggggatacgt
      601 tgaataagga ctggccaaca gatgaacctt acacatttga tgcagtagta atgaacccac
      661 cttattcggc taattggtca gcagatacga ctttcttaga tgattctcgg tttaatcgtt
      721 atgggaaatt agcgccaaaa tccaaagcag attttgcgtt tcttcttcat gggttctatc
      781 atttgaagga aacaggtacc atggccatcg tcttaccaca cggagtttta ttccgtggcg
      841 ctgcagaagg ggtcatccgt caaaaattac tagaagatgg cagtatttat gctgtgatcg
      901 gtatgcctgc gaacttgttc tttgggacat cgattccaac gacggtgatt gtgttgaaga
      961 agaatcgtca aactcgagat gtattattca ttgatgctag tcgtgaattt gttaaaggga
     1021 aaaatcaaaa taaactatcg gaagaaaaca ttcaaaaaat cctcgaaacc tacgctgaaa
     1081 gaaaagatgt cgagaaatac gctcatttgg caacctttga tgaaatcaaa gaaaatgact
     1141 ataacttaaa tatcccacgc tatgtagata ctttcgaaga agaagaacca attgatatgg
     1201 ttcatgtcgg aaatgacatc aagaagatac gacaagaaca gcaagtgcta gaaaaaaaac
     1261 ttttagaagc tatctcttcc ctgcaaacaa cacctgaaaa tgaagcgtgg ttacaaggtg
     1321 cgctggaggt gttcaaacat gagcaatgat acacaaccag aaatccgttt cccaggattt
     1381 acagatgatt gggaacagag gaagttgggg gacttcgttg tagattatgt tgaaaagaca
     1441 agtgtacaaa atcaatttcc tatgctaact tcatctcaac aaaaagggat agttttgcaa
     1501 gaagactatt ttgcaaatcg gcaagtaact acagaaaata atattggtta ctttgtacta
     1561 cctagaggat atttcacgtt ccgtagtcga agtgataatg atgtgtttgt gtttaaccgt
     1621 aatgacatta ttgatagagg gattataagc tatttttatc ctgtatttac tttaaaatct
     1681 gctgattccg acttcttctt aagacgtatt aacaatggaa tccaacgaca attatctatt
     1741 caagcagagg gaacaggtca acatgttctg tctctaaaaa agtttaaaaa tatagtagca
     1801 atgtttccta gtgaggaaga acaacaaaaa atcggcacct tcttcaaaca acttgacgac
     1861 acgatcgctc ttcatcagcg taagttagat ttgctgaagg aaacgaaaaa aggattctta
     1921 caaaaaatgt tccctgaaaa cggagcaaaa gtgccagaaa tccggtttcc tggttttacg
     1981 gaagattggg aagagcgtaa gttgggagaa ttaggtaaaa ctcaatcggg aattggattt
     2041 cctgatgccg agcaaggtgg ttctgaaggt actccatttt ttaaagtttc cgacatgaat
     2101 aacattggta atgagtatga aatgagaaat gcaaatcatt atgtatctaa tgagcaaatt
     2161 gagcgtaaaa aatggaagcc aataaaagat gtgccagcga ttatatttgc taaagttggc
     2221 gcagcaataa tgcttaatag aaaacgtttg gtcacaagcc ctttcttaat agataacaat
     2281 accatggctt atttatttaa taatacttgg gatatctatt ttggtaaaat attgtttgaa
     2341 actataaatt tgccaagata ctcccaagta ggggcattgc cttcttataa cagttctgat
     2401 attgagaata tcagtgttaa ggttcctgta aaggatgaac aacaaaaaat cggtactttc
     2461 ttcaaacagc tagatgatac gatcgctctt catcaacgta agttagattt actaaaagaa
     2521 acgaaaaaaa gcttcttaca aaagatgttt gtctaggagg gaagaagaat gaaaattagt
     2581 caccgtgagg aagcagaagt cgaagaacag ttgattcgtg tacttggaga agggcataat
     2641 cagtggacgt atcgtccgga tctaaaatca gaagaagacc tttgggtcaa tttacgccaa
     2701 aaaattatct caaataatca agcagaactg aatgattttc ctttgaccga taaagaattt
     2761 gaaacgatca aaacggaatt actgctgcga acgaaaaccc catttgatgc tgcgaaatgg
     2821 ttaaaaggtg aaaatgggat ggctcggatc acgattgaac gggaagattc gcaattgggt
     2881 tctgtctcac ttatccttta ttccaatcaa gacattggtg gaggaatctc tacttatgaa
     2941 gtggtccatc aaattgcaaa acgtggaagt aacatagaag cacgggatcg acgttttgac
     3001 gtcaccttat taatcaatgg attaccgatt gtccaaatcg agctgaaaca agtaactgca
     3061 aaagatggtg tttatcaggc atttaaccaa atcaaaaaat atgcagaaga aggtatgttt
     3121 cggaacaata tcttttcgac cttacagttg tttgtggttt ccaatgaaca aaccactcgg
     3181 tattttgcaa atgccttacc gaaagacttg caccctaagt ttttatttag ttggcgaacg
     3241 aaagacaatg aaaaagtaga aaacttatat gagttttgta aacaagtgtt aaatatcccg
     3301 gatgctcatc gtttgattgc ggattataca atcgtcagtg aagatcaaga caataaaacc
     3361 ctcatggtgt tacatcctta tcaagtacat gcgattcaag ccttgtttat tgccgcaaat
     3421 aaacatcaat cagggtatgt gtggcatgcg actggttcag gaaagacgtt gactagtttt
     3481 gtatctacta agcttttagc acgaaaatcg gggatcgatc gaacgatcat gttagtggac
     3541 cgcaaagatt tagataatca aaccaccaca gagtttacaa aatttgcttc ggaatttaat
     3601 actgggattt cttccgggaa cgccaaagcg aatagcttga tcgttggaac gggaagtgcc
     3661 aaagaattaa gtgaaaccct gttggcagat gccaatgcaa atgttgtaat cattacaacc
     3721 cgtcaaaaac tagatgcggc gttgaaatat gcaaagaaac aagaagagaa aaaaggaacc
     3781 aatcgcttcc aaaaattaat gggccaacat atcgtgtttg tagtagatga gtgtcatcga
     3841 gcgttaagtg ctgaaaatat ggaagaaatc aaaaaaatgt ttccgaaatc cacttggttt
     3901 ggctttacag gtacaccgat ttttcctgag aatcggaaac aagccaaagg gcaattggca
     3961 cgtacaaccc atgatcagta tggagaggtc ttgcatacct ataccattaa gaatgcgcta
     4021 gaagatggct ctgtcttagg atttcaagta gagcatgaaa acaccgtgga gccgacttca
     4081 ctagagaata aaatctatcg gaaattaaaa gaagtagaaa catatgcaga gtattcatca
     4141 gaacagatca atcgtatgat tgatcaaatg gaacctgtca aaaaagaaag ttatttggac
     4201 ccagctgttt ttgaagcaga tgaacatatc caaaaagtca ttcataaaat gttccgtcca
     4261 gataatgcct atacaaaatt tgattttcga aatggcaggc caacgaaatc tgcaatctta
     4321 accacgagtt ccattgacat ggccaaaaag tattatcggg ccatcaaaga gatgacgaaa
     4381 gagcctgatt ggttaacaaa agagtttccc aatcagctca ttcgtgaagg acgaacgatg
     4441 gaggatcctg attttccacg aatcgctatt acctattcat tagaagaaaa tagccgagat
     4501 tcaagtgccc aacaagaaga aatgcaaaaa attattgagg aatataatca gtattatgat
     4561 acttcctggt cattagcaga cattgagcgt tataacggag acatcaataa tcgtttggca
     4621 cgaaaaagag cggaattcaa acaatttggg aaacaaatcg atttagcgat tgtagtggat
     4681 cgtctattaa caggttttga cgcccctacg attcaaaccc tatttgtgga tcgtaatcta
     4741 gagtatgcag gattgatcca agctttttca cgtacgaatc gtacgtatcc tgataagaca
     4801 aaaggattga ttgtgacgtt tcgtaagcca gcgacaatgg agaagaatgt cgaggatgcg
     4861 acaaaactgt attcaaaagc aaaagaagaa tcaggtttga tttatccaag ttatcaggaa
     4921 tccaaaaaaa gattcaaaca agcatatggt aagttaaaag aggtttcatc catggaatcc
     4981 attgatgaac atacgccact tgaaatccgt atcgagtacg tcaaagcatt tcaagaattg
     5041 aatcgtgctt atgaatcgtt agtgacttat gacgattaca atgatgacat ggtgaaatct
     5101 ccaactttga acaatcaaat tcaacttttg gaagaacaaa ttggcgtgta cgagaccgtg
     5161 aagggttcat taatagagga agaaccagaa aaagaaggag aagatttttc aacgatcgaa
     5221 ttttatagtg aaaataccac taagttgtat gatattgatt caacttatat tgatcaatta
     5281 ctaggaactt atgctgcgaa tagttcagat gcacgagaag aaattgaaaa agctttggct
     5341 aaattgaata aaacagaagg agtcaaagaa gtttaccgac aaattttaaa tgcatttgat
     5401 gtggggactt tagatagtaa tgaagatatc tttgtcatta aacgtcgcta ttttacacaa
     5461 gcgagtgatg atttgattca tgtcttttcc aatgagtggt ttgtttctga gaaagaatta
     5521 catgcttcta atctgcaata tctaccaggt gaagatccaa ttcctaatat gaaagcaatt
     5581 atcaacagta aggattacga aggatataaa gccaaacatc cagaagcgaa gccatttaaa
     5641 tacccacaag aaatgaagcg agcatggcgt aaaatgttag atgatgagtt gattccactt
     5701 gagaatgaat tgaggtaaac gagaagagtt aagaaaattg tacgattcaa agatggtagt
     5761 caagtggaag tgtagaaaat agggatagat gtcttttatt tgttcacatt ttataacgaa
     5821 gtatttaaga cagctattga ataatgtttc ctccaccttt ttcattcatt taccccaccc
     5881 cgttgttata tgaccgtgtt ttgagaggaa ccaataaaaa gcataggcta actaggggtt
     5941 cctttttctt ttgttttttt ggaatccgct taagtgaaat gtatctgaaa tgaaacggag
     6001 ataaagtgat tttttgctat aatgacaagg ttttagctgt ttcagatacc atgtaaccca
     6061 aaatagttta ttgagaaaat aggagagtat ttgatactaa ctagtaggaa aaaatgagat
     6121 aaaaattaaa tggaatcatt tcaggtaatc tcaaataaaa aaatatacag tcctgtggca
     6181 ctttacctat gggactgtat attttttact ctcagcgggc ttgttttgac tccgtgtctg
     6241 actccgtcgt attgatttta aatgatattc gatgaccatt taacaggtca tttttgaaat
     6301 ttttgtctct atatgactca gtttgataat tattgataga tcaatttggg tctatatcga
     6361 acaagggaat aatccaataa aaaatgattt agaatatcaa aataacaaaa aagaggacta
     6421 cctgtattcg tcctcttttt tgaccagatt ttgaccagtc taatttacat tctaatatat
     6481 tcatatgagc taattttttg attaaatggt aagtttttcc ttgtttgttt tagtttaaac
     6541 tagtgtgtaa tttaaaaatc tatatggaag ggtctgtggg gtaatactta aataaaggct
     6601 aggaacctga taacatcaag gttcctagcc tttttataat aaaatgaagc gaaaataatt
     6661 tcttttaaag agccgagatt atcagctata taagagtgaa tttgatgcaa aaaacagagc
     6721 cgaaacagtc ttggtgttcg tagctctgct tatatgtttc gaatttaaga caaagagttg
     6781 gcaaaagatg ttgataagga agataagata actgtgttat ataaactagg taactaactg
     6841 taacaagcta attttaagag attcttatta gaagacgttt aggaatataa ctaagaaaat
     6901 gattatacag gtaattgtca tttcaataat gttttctaat tttcgcgtct ttgttttaag
     6961 aaaaaggcca gaatcagttg gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc
     7021 tactaatatt cccactaaca agagataaaa cggccaggtt ggtgtgattc ctctataaaa
     7081 aaatgcccat gctaccgaaa tcccatagat taacaactga acaattttta caatgaaaaa
     7141 attgttctgc cattactcaa agtctcttcc tccttgtatc gtttatttgt ttcattttat
     7201 cattatgata ggcgcttgaa caatccattt tgtattttat tgagtactct tggataaact
     7261 accagcgatt atcatattta tttatgttac tgtaagccat catataaaag taaatgagca
     7321 gatcatagaa atataaaaaa gaactaaaat tgatctaaat tatgtgcagc ttgactacgt
     7381 accaatctat attacttact agattggtcg ttaatctatt cggatcgaat gagcaaaata
     7441 tttttgtgaa tgtccatcct tgattatgat tccaaaaaga atcattggga ggctgtggcg
     7501 taaatattat actataagcc agaaatacct atcattctga acttttttat ttaggaactt
     7561 tcatctcaag gctttttcaa ttaatttgta cccctgtttt ttggataaga tcatgtgcat
     7621 tgattttttc tatctctacg ttcatctcct acttaatgcc tatctggctc ctgagggctt
     7681 ctttttaagg cgtcgtcata gtcttggctg aggatttctt ggtaatctga gggaatggct
     7741 tcccaagtga gcaggcgaac gttcatgacg tgttttcctt tgtgtttgat tacagcatgg
     7801 tttttctctt tttctacctc tccagcgtga aaagacagcc atgtaccatg gccaaagata
     7861 tcattacata cccaacgttc gatggaattg tccacgtatc ctacagggag tgtggtatag
     7921 ccgatttgtg gtttcaaaaa aggattgaat cgatcgatcg tttcattttt gtattgataa
     7981 ttgataagaa aaagatagac caaacaaaag acgagcagaa agaaagatag tgtctttttg
     8041 agtgagaagg gttgttttga tgaataagat ttcttgttaa caagaggttc atctggcaat
     8101 gttggttcta ttgattcggt agctgattca atagaaggaa aatcaatcgg cagacctaaa
     8161 gtatgatgta aaagtgacag catttcaagg tctggcattg tacgagaatt ttcccaacta
     8221 gaaatcgttt tatcggaaac accaattttt tcgcctaatt ctttttgcgt aagcttacaa
     8281 tttctgcggt aagttcgtat tttttctccg gtgttcatct tcttctcctt tcgattctac
     8341 agtatataga tttattctac atgacctata cacttgatag atacgttttt ttgatgaaaa
     8401 acggtatgat tttatcaaag aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa
     8461 gttcacagtt ttactcacaa tcatagtgac cggtatcatg agtggttttg ccttggttag
     8521 tataaataaa caggtactct atcggacaaa tgaatttttc catcaaacag atacttatac
     8581 tccgattcga ttgccacaac attttattgg gcaagaagaa tatgagcagc taatcatggt
     8641 atttgaaaat gtctcatcag aaactgagct ttcttatatg aaaaaaacag taaatgaatc
     8701 caatgaagta gatacatctt ggaacttcaa gatacagcca aagcttgaaa tgacattgta
     8761 ttatcaaggg gatgtcaaat cttctcacga gaaggtaccc tattctaata cctcctttcg
     8821 cgtaacgtcc attgaagaat cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat
     8881 gacgagagaa aaagaagcct atgaacggtt catttcacta ttcactgatc gattcaatca
     8941 agcatttcac actgattatg ccactaatat ttttgaagac tttgaaaata gctattcgta
     9001 ttcggttatt tcaaatacag acgtatataa ttatcgagtg tatttaacta ttggattgat
     9061 tcttttattc cttttgttat catccttttt cttattgatg aataaagaaa tcagtttatt
     9121 gttgaataat gacttttcat tacatcaagt attgtggtgt ttattgggga aaaagaaaat
     9181 cattattaca ttcataattg caggaatcat tggtggtttt atttttatgt attataagga
     9241 gcaatttctt tatttgttcc agatgtacgt tttcttgttc agcggaatat atggtatctc
     9301 tttgatttct cttttattag ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat
     9361 ccaatacatt ctttttacat ttggattgtt gttttctctt cttttgaccg ttgccaatgt
     9421 cgaattagca actatcgttt tttcttcttc acagttagct gtttcccatg aaccaccaaa
     9481 agaaatcgct ggtgaggact atcatgtatt ttatccagta accatcggga aaaaccacgt
     9541 ggattttatt tattccaatc gttttgcatc agcagcagat caggaattat atgaaacttt
     9601 gaataaaaac ggaagtatat tagtcaatgt atcagattac ttgaatgaag aaaatgaaca
     9661 gtttggcaga gggataaaaa taaatctgaa ttatcttaag aaatttcctt tgattgatgt
     9721 ctctggaagg ttgatccaaa taaccgaaaa agaaacgcac cctgttatat tgatccctga
     9781 acgatataga catacagatt taaaaaatga cctggcgcaa ataacagaat attatcaaga
     9841 aatcagtgaa aaagaaccga aatttctatt tataaaaaac aatcaaccca tttatacatt
     9901 tattccttct atcccctgga tcgaacatta tccggttgtt gaaatactga cattgaaaaa
     9961 cagtatgtat ttggaaagaa atattctcag cggagaaatc tatccaccgt taaagatcaa
    10021 gataggctct ttgtcgaaag aaaggttatt tgagctgatt gaagaaagtc agttacgtga
    10081 taacttgcag ttatcatttc cgtatactca gactgaagag atagctgtca aagtgttaag
    10141 cagatcattt cattacttga tacagatatt tttactcact ttcttttctt tcttcttgtt
    10201 gagctatgtc atgttatgca tatattttgt ctacaattgt cggaaaatcg ctatctttcg
    10261 ttcaagtggt tattcgctct ttgaaacgta taaagatttc ttcatgatga acctcatcaa
    10321 atggggaacg acaagtgtaa tcttcctttt tcttatagaa agagagccta aatatctttt
    10381 taatattttt ttctttagct tcattgattt tattctttca gttgttttta ttttgtctac
    10441 agaaaagaaa agtcagttat tgctgatgaa tggaggttaa caattggagc cgttgatagt
    10501 gataaaaggt gcgaaaaaaa tcattcgaaa caaaacgatt cttcatttgg atcgtttcac
    10561 tgcggatgct ggggatcttg tgactgttgt tggagaaagt ggagcaggta aaagtacgtt
    10621 attgaatata ttaggattga atgatcagtt cagttcagga caattctatt tgttcgatca
    10681 agagacgaaa caattaactg ggagagagaa gcgcttgata aaaagaaata ccatatcttt
    10741 tctttttcaa gattttgggt tgattgaata tgaaacaatc gattttaatt tagatattgg
    10801 attgaagttt tcgaatttaa atgtttcaga taagcgaaca gcaaagtatg aggcattgaa
    10861 aaaagtaaat ataaacaaga agctaagtac gcctatctac acattatctg gtggtgagaa
    10921 gcaaagaatc gcctgtgcga gagtcttatt gaagaatagc aaaatcattt tagccgatga
    10981 accgactggg tcattggata aaagaaataa ggataaagta gttgaaatct tatcgcaaca
    11041 agcttcccaa ggtacgttag ttatcgtagt cacgcatgat ccatatctga ccaaaatagg
    11101 aaacaagaag atcatgcttt aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc
    11161 agttattatt tgtgcactag ggataggttt tgttggagga aacgctatgt catctgaagt
    11221 ggaagcaaat tcagttcacg gtgaaatcta ttacaaatct ttaaagccag gagagattca
    11281 ttttgatgct aaaggtaata ttattaagtc aaatttacca gtagaccggg aaatttctag
    11341 aacagcaatt aatcatagct ctggaagatg ggtttattat agtgatacaa cttgtacgag
    11401 aaaaacaggt cattctaacc actatagtcg tgtctataat cgtcacggtg caaaagcgaa
    11461 agttggaaac tctattcgat cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc
    11521 gacaggaaat agaactgaca cattctcatg ttggtacaat ccaaccggtt ggtattaagt
    11581 atccaaaagt ttatataaaa tagaaaaaac atataatgaa acagcgttaa aagttatttt
    11641 tggtagtttt aatatattca tttttagttg tataaaaata tatcataata aaaacaacaa
    11701 aaaaatgtct ggaaaatcga acataaaagt gtctgatttg aatatcccag cagcggaaga
    11761 agaaaaacaa aatcgataac atttgttcta agaattagtt acgttaaata cggaacgcaa
    11821 gatagacttg aaaaacggta acaaaagaag tagtatatta aggtgtgatg tgctacttga
    11881 catttattct gctgagcgaa ctttctggat tgtgtagcct tatatctatt caggacgtat
    11941 cgagcagatt agtaaaggtg ttcttgatga ttttcatctt gaacaccttt gtttttagca
    12001 attcgcaaaa aacgaaaagt aggagaattg ataaatgaaa aaaagatttt tagcacttgc
    12061 catcgtacta ggcactggct tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt
    12121 tagttataaa ggcggtacaa tcagcgaaca agaagtaatg gatagcttga aaaaaatgca
    12181 aggagcagat tccgctgtgc aacagttgat cgtttatcaa gtatttgaag ataaatatgg
    12241 agatgacgta tcaaccaaag aaatcgattc tcaatatgat caaacaaaga aacaattggg
    12301 tgattcattt gattcacaat tgaaatctgc aggttacaca gaacaaacat tcaaagacag
    12361 tatcaaacaa agtctagcct ttcaagaagg tctaaagaaa catatcaaat tgaccgatga
    12421 agatttaaaa acggcttggg aatctttcca tccagaagtg gaagctcaaa ttatccaagt
    12481 agcaagtgaa gatgatgcaa aagatgtgaa gaaagctgct gataagggag acgatttctc
    12541 taaattagcc aaagacaagt caactgatac tacgacaaaa gaagatggcg gaaaagtgaa
    12601 atttgattcc actactacaa cggttccggc agaagtcaaa gaagcagcct ttaagttgaa
    12661 agatggtcaa gtttctgatg tgatcacttc taccaatgct tcaacttata ctacggaata
    12721 ttatgtggtt aaaatggtga aaaaccaaaa caaaggcaac gacatggata aatacaaaaa
    12781 agaattgaaa gaaattgcaa cagacacaaa attaagtgac agtaccttcc agaataaagt
    12841 aattggcgaa gtattgaaag acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt
    12901 tttgagtaca ttcacttcag attctagtac agcaagtagt aaataagcgg tataaataaa
    12961 aaatatgaac tatttaaatc aataaaactg ttcatctaaa tctttttatt agaactaaat
    13021 caacagta
//
LOCUS       DAZULO010000074        12995 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000074 DAZULO010000000
VERSION     DAZULO010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.076"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            266..472
                     /locus_tag="ACG8OA_002089"
     CDS             266..472
                     /locus_tag="ACG8OA_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFS4976852.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(674..1675)
                     /locus_tag="ACG8OA_002090"
     CDS             complement(674..1675)
                     /locus_tag="ACG8OA_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4976853.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1680..3593)
                     /locus_tag="ACG8OA_002091"
     CDS             complement(1680..3593)
                     /locus_tag="ACG8OA_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4976854.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3761..4267)
                     /locus_tag="ACG8OA_002092"
     CDS             complement(3761..4267)
                     /locus_tag="ACG8OA_002092"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS4976855.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4427..4867
                     /locus_tag="ACG8OA_002093"
     CDS             4427..4867
                     /locus_tag="ACG8OA_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4976856.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            5026..6366
                     /locus_tag="ACG8OA_002094"
     CDS             5026..6366
                     /locus_tag="ACG8OA_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4976857.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(6435..7463)
                     /locus_tag="ACG8OA_002095"
     CDS             complement(6435..7463)
                     /locus_tag="ACG8OA_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976858.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(7518..7592)
                     /locus_tag="ACG8OA_002096"
                     /pseudo
     CDS             complement(7518..7592)
                     /locus_tag="ACG8OA_002096"
                     /inference="COORDINATES: protein motif:HMM:NF016901.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7595..7951)
                     /locus_tag="ACG8OA_002097"
     CDS             complement(7595..7951)
                     /locus_tag="ACG8OA_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976859.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(8249..9223)
                     /gene="sgrA"
                     /locus_tag="ACG8OA_002098"
     CDS             complement(8249..9223)
                     /gene="sgrA"
                     /locus_tag="ACG8OA_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFS4976860.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(9419..10258)
                     /locus_tag="ACG8OA_002099"
     CDS             complement(9419..10258)
                     /locus_tag="ACG8OA_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS4976861.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            10443..11330
                     /locus_tag="ACG8OA_002100"
     CDS             10443..11330
                     /locus_tag="ACG8OA_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFS4976862.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(11914..12609)
                     /locus_tag="ACG8OA_002101"
     CDS             complement(11914..12609)
                     /locus_tag="ACG8OA_002101"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFS4976863.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(12593..12991)
                     /locus_tag="ACG8OA_002102"
     CDS             complement(12593..12991)
                     /locus_tag="ACG8OA_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS4976864.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3888 a         2634 c         2015 g         4458 t
ORIGIN      
        1 tttaggatac atcgaatttt tggggaaatt cttttggggg tatcctaaat cttttctatt
       61 atattcccat attttctatt attggtctag acgtatgatg gtaaactttg tacctatctg
      121 attgctgaat cattaattgc ttatcattag ccatctttgt ttcaagttcg ctagatgatc
      181 attttattca catcctaaca aacttgtcac atttatagat tcttaatcta tttatcatac
      241 aattataaaa aaggaggtgc tgcttatgaa agctatccat atttctttgc tttttttcgc
      301 tagtgcattg attttatttg ctattcatct ttgttcccag ccttatatca ataacaacgg
      361 aatattagtg gaaccagcat tcttttgctt accactcggc tttttgtccc ttcttgcctc
      421 cgcaggcttc ggatttgttg ctttttttaa acgatcaaaa caacaaatct gacttataaa
      481 atttacgtaa aagagatcgt gacaataatc gtgtgatatt cccaaatttc gaataaaagg
      541 tgccccttgc catctctaac tcctcagtca caagttacaa tattcataaa ccatgaaaag
      601 cttgtttatt gaaattgctc cttgtgactt ggagctaagt gacagattac aacctctttt
      661 gcatcataag actttactcc ttgaaaaaca ttactaactt ttgatatgtt tcttgcgctt
      721 gtttattgct gaaatcaaac tgatattcat ggttcaaaac agcatttttc tccgcataat
      781 ctacttttgt gactttcacc cccgctttct caaatacaga agttacttct tccatttgtt
      841 tcgtaaacgt atttgtattt ccatccgtta agaatgtgtt ttgcgtccaa cgatccgcat
      901 gtctgaccaa ggctaaactt ttgaccttct ctgattgttc aaagtcctta ttatcaaagt
      961 aagcccatcc acatttttca taaagaaagc tagagatttt atcacttact tgagtaagtg
     1021 cagaaggatc caacagacca gacaaagaaa taaacttcgt aatctgcttg ttctccaaag
     1081 tcggatcaag atgatcagct tttttaattt cttcattttg aagtgctaac aaatattctc
     1141 cactaatttg tgcacctgcc gaatttccac caatcgaaat attcgaccaa tctgcttgat
     1201 cttttatctc ttttttagct gcttgaatgg cttcgttcaa ttggataact ggtgtaggat
     1261 gttttgcttc cggtgatcgt tcataatcta tatttacgat gatttgctgc gtgtctgaac
     1321 atagatattc caaataatca cgacaatcat ttttatcccc tcctacatat gcgcctccat
     1381 gcatccagaa aagaatcggt aattttttct tttccacttt cggtgtgtag atatccatat
     1441 tattattttt gtactttgaa ggataggata tttctttttt tatatcgatt tgttcgattt
     1501 tttgctggaa atcatctggt cgggcatata cttgttcttt ttcacttgtg tcaaattgtt
     1561 ttctaacaag caagctaatc ggctttggtg tgatttgatt aatcaaaaat acagtgacta
     1621 aaataactac gatgataact ggtactgcaa tcactattcg cttgaaccat ttcattcatt
     1681 taccctctct ttcttcgcca aatccatgtg ataatcgtgc taacagataa aaatccacct
     1741 gaataaatga gaagcgaatg tttcttttct ccagttttcg gcaacattct tttttttgca
     1801 tctgtctttt tttgtacttt cactgtcatc tgaatcggtt gcgtatcatc acttgattga
     1861 tcctctcgag gttcattctt tcccttattg tctgcattct ctgtcggatc ttgctttttt
     1921 ggtggagcta ctgggatttt tttgtaatag aaaataatag ttggtatcgt ctcattaaaa
     1981 atcccttcag tcggtccttc aacatttgct aattcgtatc cttcgaatgt cttttgctct
     2041 gtcatataat tttctccata agctccgtaa agctggattt cgtcctggtc tttgagtaga
     2101 cttttattgt tttcatccac atactttacg tttatctgat catggaacac tactttcaaa
     2161 aagtccgtta catgtttcgt gtaggcagca tggtcaactt cctgtgcctt tccatgccct
     2221 gctccttcta ctaataaaag atctttatac cctcctgata actcatataa tttttgcgcg
     2281 ttagacacag gtactacatc atctgctgtt ccatggataa tcaatttagg caatgaatct
     2341 gacattccaa tcgtaggtag agcagctttt gtatccaaat gcatctcctg atcaaaatag
     2401 tattgtttca aaaggcccat aaataaatct tcatgctctt gatccccaat gatgtctaac
     2461 tgatttttta tggcatccgg tagtgcgggg gctactgtct ggctgtacgt aaaacgtagt
     2521 tgttcatcaa ttgatgcaaa tccgcaattt tcaataattc ctttgacatt gacaggaata
     2581 tctttcgcta atacagacat catcgtagct gctcccatcg aaccaccata aagtaaaatc
     2641 ttttgagatg gccgctctcg aacttcttga tttatccagt aaaggacgtc atctgactca
     2701 tattgtccat aggtcacata atcgcctccg ctattaccag tcgctctgga atcaacaaat
     2761 aataaattat acccggcctt ataaaaatca ttgtattctt ctgtaacgat cccgttattc
     2821 caattccctc gaaatccgcc atggatcatg actgttttat ctgaaccttg atcgacataa
     2881 tatgcatata catccatcat tttgccatct tgtttgttta gtacagtttg atgaagttgt
     2941 tttgtatttg gatagaagcc tgtgttttct gtaggcgttg tcccatcatc tgtacctcca
     3001 agcatcgaca tcaacacact gttttttgca aaagcgatat cgtacaatag ataagtagca
     3061 atcagatgtt tgataatctc tttatctaca ccataatcat taacagcaag ctcagtgact
     3121 aataactcta ttaattttga cttgatgtct tctgttggtc cattaagtat ttctgtcatc
     3181 tgtttaccga agtaattcaa atattcttta ccattgaaat tttcaccttt accgatatca
     3241 tcattttctg tcccattctc tttgtcactt actttagcag aagaggattc gtcgtttgac
     3301 atttttgtag ctgttgcttc ttctgtttgt tggttatctg tggtagcggt cgtttcttga
     3361 tctgtgctag taacagatgt ctcaaccgtt gtttcagtat ttgtttctgt ttgtgaagta
     3421 ctcgatttcg tttcatttcc agcatcagta gtttctgtgg tttctacctc ccgagattcc
     3481 gattcggaag aaatgctctc tccatatact gtccattgcg ggacgcttgt acttacagca
     3541 aataagatga ccaacgctcc ctttaatact tgttttgttc tcaagctatt cactcctttt
     3601 tatgtttcta ggagaattat atgtaaacgc aaacattttt tatacaaaaa ataaatggtt
     3661 tatcccataa aaatagaaca ggattatttt ctcattttac gtaataaagc agaatggata
     3721 aaccagtatc cattctgctt tttttaaacg aagaaacaac ttattgctcc aaaaatgtca
     3781 gaaatcgttt atcccaaatt ttctcgtaaa aagcgattgt ttcctttgct ttcataaaag
     3841 gattatcata agttgtcgtt gctcctctac acatcataag ctggtatcct aatccatgag
     3901 caaacttcac agtcgtatct atacaatact ctgcttgtgc cccacataat tcgatcgaat
     3961 gacagtccaa ttcagttaat aaaacactca aattagtctt ataaaatgaa ttcgcatggg
     4021 tttttctcac aaaataactg tgctcaggaa tagataattc cgacaatagc tcccatggtt
     4081 ttgtttgtgg cactaattga gcatcttcgt gttgaacgaa aataactggt ttcccaatcg
     4141 tttcgtatgt tttgattctt ttattcacta atgctgtcac tgtttctaat tcgtgtatcg
     4201 gcgaagaatc aaaacatacc cctttttgta aatcgatcac tactattgcg tcggctgtgt
     4261 ttgacatact tgtgccttct ttcttttttc ttgatttaat agtatttctt atctagctag
     4321 ttccattata cgtgacaaca ttttcttatt ttacacctct aaatggcact tttcgactat
     4381 ttcttcatgt tattatttca taaatactta gctattggag gaatgtatga tgattaaatt
     4441 gtatacaggt tctggctgct cgtcttcaag acgcgcaaaa cgttggctta tcgtccatga
     4501 tataccgttt atcgaacaga acttaattcg tcaaccacta accaaaaatg aatttatcca
     4561 cttactaaaa ttgacaaaca acggcgtgac cgaaatgctc tctactcatt ctattagtta
     4621 taaaaatctt gttcacagaa tagatgaact cagtttgagt gaactatata ttgagattcg
     4681 tacatcccct tctttattga aaaaaccgat tatctacgac caatctgcaa aacttttgac
     4741 tggatttagc gaagataaaa tcaggatgtt cctttctaaa actaaaagaa agcaagagct
     4801 caacgaactc ttgctttctg gtcatcttgt cgattttaaa gaaaataatc tttttgcaat
     4861 gtcttaattt tggcataaac aatctgcttt tctggatcta taatatctag tgttggtgac
     4921 gccttctgtt gccaatactt tttctttttt ctaataaacg caaacagaca ccttgaaatc
     4981 ctttagaata aagtcgacga aaaccattca aaggagtgtt tcaagatgtc ctattcagag
     5041 tttaccaaag agttactaga tattctagac ctaaatctta cctttcatga agatgccttt
     5101 cgcaaagagc gcattaatga cgaaacatgt tttgtttttg atggcacatt gacctatcaa
     5161 ccagaggaat gcttccattg ccactatcaa aataagcaaa cgatcattaa atggggctgg
     5221 aaaaaagtat ccatcttatt gaatgatgtc agtaactaca agaccattgt gcggattaac
     5281 aaacagcgct tcaaatgtaa acactgtgga aagacttttt tagcagaaga ttcagttagc
     5341 gatcgtcgct gctccattgc ccgcagagtc aaacaagcca ttcttgagct attaagtgag
     5401 cctatttcta tgtctttgat tgcacgtatg aagcacattt ctccgaccac tgtcattcga
     5461 atacttcgca gccttcggcc taaaaccgtt tctttgaacc agccgttacc agaagtggtg
     5521 tgtttcgatg aattcaaatc ggtgaaaaac gtctcgggag ccatgagctt tgtcatgatg
     5581 gacggcaaga cccatcaact gattgatatt gtggaaaatc ggcaactcaa tcctttacgc
     5641 gactatttct tgcgctaccc tagaaaagtc cgtgaacaag tacgtctagt ggtttcagat
     5701 ttctatacac cctatcgcac attggtgaaa gagtgttttc cgaatgcccg aatcgtcgcc
     5761 gatcgctttc atattagcca acacatcggt cgggccttta ccaaccaccg aattcaagta
     5821 atgaagacct ttaaaaaagg ggatcgtcgg tcgaagcact taaagaaata ctggagacta
     5881 cttcaaaaga atgcgtggga attgaatggt cagcatcgct actggcgtcc gtcatttcga
     5941 gatcatctga cagaagcgga gatagtcgat cggctattat attatgatga ctccttaaag
     6001 cgaggctacg aagtctacca ggtttttctt tccgctatca gaagacaaga tgttcctgaa
     6061 ttcgtcgcgc tcctaaaaga agactacaag gagttgccag agcactatca accagtgttc
     6121 acgaccttca agaaatatcg aacagaaatc aaacgagcgt tgcgagttcc ctattccaac
     6181 ggccctatag aatgtttgaa caaccatatc aaagtactaa aacgaattgc ctatggcttt
     6241 agaaactttc aaaactaccg agaacgaatc tttttatatc gaggaaaata tttcaagaaa
     6301 actaagaaca ctacccaact gactaaagcc agaacaacaa cacggctaga caagatagct
     6361 gtctagccgt gtgagtatct cgattattct attgaatttc ttcaccaaca ctcattgacg
     6421 aagagccgct tttcttaatt aacgaaatcc aatttttcca atatggatct atttttctca
     6481 ttcctgattt ttatgatttc agctttcaga cgcgcccaat cattttttgt aggttgtcca
     6541 ttccagtaaa atatctgtgt attcgtttga tcatatgcct ttttgtcgat aggataatcg
     6601 ctaatgacaa tatcaggtaa ctgatcagcg gatgcactat attttattgg gcgttcagtc
     6661 atagataata tccttttctt tgcccataat tcctgtgttt taccatactt tgattgaata
     6721 tagaatatga caggttgttc gtgttttata aaaatatccc tgactaacat acagtattga
     6781 aaaatcaacc gtctgttctt ttcataataa gtattaagct ctggaattct gcgagctacc
     6841 ccactaaaaa actgttgcat gattggatac atcactggaa atatatgctg aaaatctctg
     6901 tcttctgctg attttccaaa agcatccacc ttcttttttg tcccaaaaac ccttgaatac
     6961 aaatggatca ttgccaaatt gacatataga taattttttt ctgaagttgt aaaacaaatc
     7021 ttgaagtttt tttcaaaatt ttctacaata gacgattcaa tccagcccca actttcttgt
     7081 actttatctg gagtatccaa caaatcttct tccaaatagg tgttcattac accaaacata
     7141 aaataaaggt aagcttgctc tgttggagtg atgtgattca tcgaccagaa gtttgcttca
     7201 aaaaaaggct ctagtatgta atgcgtaaac tcttcttttg ttatcaatgg atgcacaaat
     7261 ttgttcatct cttctgggac atacgtaatc gagtggcctt gtttcattcg ttgcaacgaa
     7321 attcttagta ggctgatcca tttctttcta tctatactac gcgtatatgg cgcatgtttc
     7381 gcaaattttt ctaatagttg acgtttattt tttatacttg ataatatatc agaatgattt
     7441 tcgtcgaatg actgccaata catcaagtga aaaaagtatc ggatacggta ttcttctcct
     7501 tctatcggat tacctttttt caagctgcaa gataaatgat agtttttcaa aatagtatta
     7561 agttctctta attttctttg aatcgtacgc aaacttatat aattttttga ctaagatttt
     7621 ctaaggattg ataagtacct ttgtaaagtg aatccagtaa aatataacca atcgttccct
     7681 ctaaaagtat agaataaact ttactcagta aaaactgaga agaaaactcg acttttatca
     7741 tcttgttttc tgaaagaaaa gataatttat attctggata cctttcgcaa aacgattctg
     7801 cttgttgaat caccgaattc atcttagcca gggtgtagtt tagatctaaa gcgatatcct
     7861 tcaccacata taatttctcg tctctttcat aacaatagtt caaaaattga tatacttgcc
     7921 tttcattgat atcaataaat tcttcaaaca aagtacatac tcctttttat ctattattta
     7981 gacttattcc gacagctaca tgaataacgt acaaatagat tcgttactga tttttcagag
     8041 atactaccat gcgcagtgtg cattgtcaat tattcttgct cttattttcc atttgtgttc
     8101 ctgttatttc ctgtttctca aatatgttag taaacaaaaa tagatctggc aaagctacaa
     8161 cttcgccagg caatacagca ctctcgaatg aaatattcac tcaaaaatct atcgttttat
     8221 tcaattttag atctgttgct tttattcttt atttcactcg atggaagaga gaacgaaaat
     8281 agcttgctcc tacaatcaaa atgagtaaca cacccatcca tgtaattagc ttgttcaagg
     8341 ttctactacc agtttctggt aaatcgcttt ttctattatc tgtttctttc gctttttctt
     8401 gatgatcaat tccacgagaa acaaaatcag gctgttttag cacacttttt aggttgctcc
     8461 ctgcatttct cttttgttcc ttagttggta tgataggtac caaatcaagt tcattattta
     8521 cttttcctgt gctgctttcg ctgctcgtgc ttcctgtgct actctcacta ctcatgcttc
     8581 ctgtgctact ttcactactc gtactctccg tactgctttc gctgctcgtg cttcctgtgc
     8641 tactttcact actcgtactc tccgtactgc tttcgctgct cgtgttttct gtgctacttt
     8701 cactgctcgt actctctgta ctgctttcac cgctcgtgtt ttctgtactg ctttcactac
     8761 ttgtgctatc cgtactgctt ggcggtccaa caactgtcca actgcttggt ttcaaattct
     8821 ccgtgatcgt catctgtcca tcataaaaaa caatcaaatc aactttatct ttactcttga
     8881 tctcatcaat cgatagttct gcagaaatca ttattgtata ctcaatacca gcggtccatt
     8941 gtacgtcttt taaataaact tccccaataa ctttttctga acttccatta ccatcccaac
     9001 catatgttag aacagcatgt acatcactag cattcacgct tttgtttggt ttgactggaa
     9061 tagtaatgac ttgttgtgtc atgtcataaa caatccgatc agttttcaca gtaaatgaaa
     9121 gattctcatt tgcccgttca tttgcagaag tactgattcc aatcattgct gcaattatac
     9181 tcagcccaag aataacaaat aatacggtcg ctgttttctt catttctatc actctccata
     9241 taattacctt ctatttgata aaggaattat ataaaatgaa aatgatgaga acacgacagt
     9301 cagttgtgaa aatctaacaa aagatgtcgt gtattcgctt gaatttgttt actttttcgt
     9361 ttaatcaatg acagacttaa aaaagcaaga ccgatatcag ccttgctttc ttcatctttt
     9421 attgataaac agcagtgtat ttcttcatcc gctttgtttt atatgcatag agtaacaaac
     9481 caacaatcaa gactaacaag aataatgtag ttgatataga tgcttcagcc ccaaagtttc
     9541 ccccagtaat tattgtattt tgcgattgga tcagagtgga gaaaaaacct tgttcttgcg
     9601 ttccgctgac tggtataccc aaaatagctc cttgtgtcca attccaagta atatgagcac
     9661 caatagccgt aaataaactt cctgtactgt agataagtgc gctgaaaaag caacctgcta
     9721 aaaagatatt gaaaactgcc agaaatgtca tgttaggatt caatccgtgc aaaagagcaa
     9781 ataatgcagc agatccaatc attgctatta acggaccaaa tctaagcatc tgatgctgaa
     9841 aaaagccacg gaataaaagc tcttctgatg cccccactgc aataaataaa ataaaactcg
     9901 ttatcagcgg cacaatgttt gcgtttcctg aatctaaccg atacgccccc gtcaaatata
     9961 atggactagt aataagcaga aacactccaa ttccaacaac gagacctccc gctaaatacc
    10021 cccagtcttt tcctcgaaca gaaacaccat aatcttctat tctttttcct tcgaccttac
    10081 tagcaagaaa taaagctagt aaaaaaacag ctaaatgtcc gaccgtaccg gcaattgctc
    10141 caatccattc actatttcca ataaacatat ccgcaagtgc attattgaca agaattccta
    10201 aaacaaaacc aactaagact attaaaacag atccaatcaa ccgaataatc ctgatcatct
    10261 ttcatccctc ctttatatta agtttaacaa cccttttctt tcttgtctcg tgaaaactat
    10321 gtaaatgttc tccatcatag agagaataga tttttacgaa ctaagaatat ctacagcaac
    10381 cttacgaaaa tgaaagactg tctccctaat tcttctgtta gactgttttt aataaggaaa
    10441 ggatgggaaa ttttttgaaa aaacaactac taattatgtg cctttcagca tgtgcaattt
    10501 tactattctc aggttgtagc aagtccaatg aaacgattgc aacgtttaaa ggtggaacac
    10561 ttacagttca ggatttttat gataacccaa aagttaaatc aaataatcaa acactattaa
    10621 aaaaaatgat tgtcttcaaa gcatttgatg atgtatacgg aaaagagatt tcttcaaagg
    10681 aagtaacaaa agaatataat gaacaaatca aaaaactcgg tccaaactac aaggaacaat
    10741 taaaagcagt tggccaaaca gaagagacat ataagaaact ctttaaacag atgctggctt
    10801 tccaatacgg tttgaaagcc aatgtaaaat taacagataa agatctagac actgcttgga
    10861 aagaattcta tccagaagtt agtacacaga tcattctatt ttcgactaaa gaagaagcag
    10921 ataaagcaaa aaaagaagca aatgaagggg aaaatttctc caagcttgtt caagcttatg
    10981 gaaaaaataa aacattaaag gaaactgatg gaaaaatgaa ttttgattca accaatccag
    11041 agattcctac tgaagtaaaa aaagcagcat ttaaactaaa aaacggggaa gtttctgata
    11101 tcattcctgt aacagatcca acgacttatc agcaaagcta ttatctagta aaaatggtaa
    11161 aaaaacagga taaaggttct aataaagaca aatacaaatc tgagctagaa aaaattgcta
    11221 ccgaagcaaa aactcttgat acggaattca tggataaaac aatccgtaaa gtgatgaaaa
    11281 aagacaatgt aactattaaa gatccttatg taaaaaatat attcaaataa acaaatttga
    11341 cccaattggg gatgtggcaa aatttgagaa gtgatttttg gaaattcctt cttatttttg
    11401 aggcaaacca cttttgccac aacttcatcg gggccttttt ttatttgtac caaaacaaat
    11461 cactggattt gttacaatca tgaggatata atttaaaaaa gttttttatt tctatcacat
    11521 gaatcaatgc gccattaagt agattctttc atgcttccct attttttcac ttgccgtatc
    11581 gcttcaaaaa caactatcat aaactcaaac tcgactgaaa agttatttta aaaaagacta
    11641 aaaatatccc ttctggactt gttcctctta ttctgttata ctagagaaac acgaaagata
    11701 tttttatgcg acacttttca tttgttttta tgaaatttat tcaaaactgg cgtaacttca
    11761 gagttacaag gatgtattag aggttaggga atacatcgtt taaatgagaa ataagtaata
    11821 gtcacccaaa ctttaaatta atgaatattg aatcgtcaga tagcctcctc gagttttcga
    11881 gaaggctatc gtttatttta tggagaaatt gattcagaag taatcagcga ccttttttag
    11941 ttcaccaatc gtttctgctt ggaaagtttg cacccaatct tcttggaagc ccctcactgc
    12001 tttatcaatc gagggcatct ctaatacttg atctagtagc tctttaccta acgttatcgc
    12061 atgagctcca gatcgaatgg ctgtagtgac ttgttcacaa tttttgaaac tagctgctaa
    12121 tatttttgtt ggtctttgcg tacgttcgat ctcagtccgc aacgaatcaa tcactttcat
    12181 agcatcaatg ccttgatttt gcatcctgtt ataataaggt gcaatataat ctgctccatt
    12241 attaatagct aaataccctt gtaattctgt gtaaatagct gtaccggtaa tcaaataacc
    12301 ttcttttttc aactttttga tcgcttgaag gcctgcttga ttcaccggca ccttgataaa
    12361 cgtatctttt cccagttctt ctatgataac tcgagcatcc tctatcattt cttcttccgt
    12421 tcttcctatt gtctgaatat ggataggaat ctgtccgatt atttttttga tttctttcat
    12481 atgcgagaag aagtttgacg ctccttcttt tttcagaatt gtaggatttg ttgttacgcc
    12541 tgttaaattc aaaaactgtt gacaagaata aatacttttt gtatttgctg tatcaagcat
    12601 gatttccata tagctttccc ccattctgat cctcttgatc gattttaatg attgtcccct
    12661 ttcttaaatg cggcatttcc ttatcttcta catacattgt tcctggaagt tccggctcat
    12721 gagatccatc aaattttatc gtgatatgtc ctaattcatt caaattagtt tggacaacat
    12781 ctcctactgc tgtgattaca tacatttgtt tatcaaaaca gagcttcata tcacaagtta
    12841 tttcttttct tacctgattc aattcaatct tataacaata atctgttaat gtttccggtg
    12901 cttcatctcc aaataaaatc attatttttt ctttcctgaa tacttctgct tctataccaa
    12961 catctgttac aaccgtttca aagatcgtca tctct
//
LOCUS       DAZULO010000075        12945 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000075 DAZULO010000000
VERSION     DAZULO010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.077"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(64..1173)
                     /gene="rpoD"
                     /locus_tag="ACG8OA_002103"
     CDS             complement(64..1173)
                     /gene="rpoD"
                     /locus_tag="ACG8OA_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFS4976865.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1197..3038)
                     /gene="dnaG"
                     /locus_tag="ACG8OA_002104"
     CDS             complement(1197..3038)
                     /gene="dnaG"
                     /locus_tag="ACG8OA_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFS4976866.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3220..5535)
                     /locus_tag="ACG8OA_002105"
     CDS             complement(3220..5535)
                     /locus_tag="ACG8OA_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4976867.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5720..6580)
                     /locus_tag="ACG8OA_002106"
     CDS             complement(5720..6580)
                     /locus_tag="ACG8OA_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFS4976868.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6639..7271)
                     /locus_tag="ACG8OA_002107"
                     /pseudo
     CDS             complement(6639..7271)
                     /locus_tag="ACG8OA_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            7438..8199
                     /locus_tag="ACG8OA_002108"
     CDS             7438..8199
                     /locus_tag="ACG8OA_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4976869.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8225..10816
                     /gene="mprF"
                     /locus_tag="ACG8OA_002109"
     CDS             8225..10816
                     /gene="mprF"
                     /locus_tag="ACG8OA_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS4976870.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11004..12191)
                     /gene="tuf"
                     /locus_tag="ACG8OA_002110"
     CDS             complement(11004..12191)
                     /gene="tuf"
                     /locus_tag="ACG8OA_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS4976871.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12559..12915
                     /locus_tag="ACG8OA_002111"
     CDS             12559..12915
                     /locus_tag="ACG8OA_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4976872.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         3713 a         2708 c         2300 g         4224 t
ORIGIN      
        1 tttttttctt ttaaaataaa ccaaacagtt ttactgattt ctcttctggt caatagtcca
       61 cgcttattct aagaaatctt ttaattgttt tgaacgagaa ggatggcgca attttcttaa
      121 tgctttagcc tcgatctgac gaatacgttc ccgagtaaca ccaaatactt tccctacttc
      181 ttccaatgtt ctcgtccggc catcatctaa accaaaacgt aagcgaagga cattctcttc
      241 ccgatcagtc aatgtatcta gtacatcttc caattgttct tttagtaact cataagctgc
      301 gtgttctgct ggacttgttg cttcctggtc ttctatgaaa tcacctaagt gagagtcatc
      361 ttcttcaccg attggcgttt caagggaaac aggttcttgc gcgattttca agatttctcg
      421 gactttttct gttggaagat ccatttccgc accgatttct tctggcgtag gttctcttcc
      481 aaggtcttgc agcaattgac gttggatacg tatcaattta ttaattgttt caaccatatg
      541 aactggaata cggatcgtac gtgcttggtc agcgattgct cgagtgatgg cttgacgaat
      601 ccaccatgta gcataggttg aaaacttaaa tcctttgcgg tagtcaaatt tttcaactgc
      661 cttcatcagt cccatgtttc cttcttggat caagtcaagg aactgcatgc ctcgtcctac
      721 ataacgttta gcgatactta ctactaaacg aaggtttgct tctgccagac gctgtttcgc
      781 ttcttggtcg ccttcttcga ttttcaatgc taacgctact tcttcttccg cagttaaaag
      841 ggagacacgg ccaatttctt tcagatacat acgaaccggg tcattgattt ttacaccggt
      901 tggcgcagaa agatcttctg tttttgcttg ttcagctttt ttctcagcac tcttcaagct
      961 gtgaattgat ggatcaccat tttcatccac cacactgata cctgcgtctt caatcttttg
     1021 gatcaatttg tccatttcat ctgcattcaa agtgaatggt gtagcaagct gattggataa
     1081 atcatcatat acaacttgtc gtttgggttt gttttctttg atgaaagctg ctactgctgc
     1141 ttcatacgtt tttttatctg ttccatttgc cataaaagaa ggcccccctt gagtagctat
     1201 ttggcttgtt ttaactgttt cgttaaattg atgatttcaa tcgtcaattc aagctcaagc
     1261 tgtttgtttc cagttcgtct ggcttcttgc tgcatttgtt tttttaatgt tatttcatca
     1321 gctaagccag atttgctgat cacatctagc aagtcttgga tttctcgtgg cgaactttct
     1381 tctgccaccg gtaaatattc gatttccacc accaaccgcc ttaaattttc atcttgcaaa
     1441 aagtctaaaa actctgcaag gttgaattca taatgttgtg agacatatgc atcgaataaa
     1501 aaataaactt ctgcatatgc atcatgaata aactgaaatt ctcgttcttt taaaatatta
     1561 cggacagaaa cttctttaaa tgcacgatat aatagaatct gctctgcctt ttctacttgc
     1621 gtcaaaggtc tctttttccg tgcagtttct tgtctcgtaa cctgagtgtg ttgactttcc
     1681 tgaggttctt gacgattttg ctgatttgtc cttcgaagat cgcgcagttg tttttgtatc
     1741 gtctctctgc cgatctgaaa ctctgttgac agttgagata gatacatatc ttgttcgatt
     1801 ggtgaaacaa ctcggctaag ttcaactagt aattcttcga ggtattcgat cttgtctttt
     1861 tcattatcta aatttttccc ttgttttaaa taacgtgatt taaaagtaaa gactgtttcg
     1921 cgcccatgag ccactaattc ttggaaagat gcttctccat acttacggag atattcatca
     1981 ggatcaagct tttctggaac gactacgaca gaaagtgcta atcgactgtt ttgccgaagc
     2041 aattcaatcc ctcgattggt tgcttcaaaa cctgcgttgt ctccgtcata gcaaaagacc
     2101 aattctttcg tcagcttttc catttgttgg atctgttgag ctgttagact tgtccccata
     2161 gaagctaatc cagttttgat accagcctgc catgcagcaa tcacatccat aaacccttcg
     2221 aacaacaaaa tttctccgtt tttccggata tcgcttcttg ctttatcaag attaaataaa
     2281 acttgtcttt tattgaacag ttctgtttct ggactattca agtatttggg ctgatctttt
     2341 tcctgccctt tttcaggtgc aagccagcgt cctgaaaaac cgatcgtctg gccttgagaa
     2401 ttacgaagtg gaaacatcac tctttgataa aaacgatcag acatacggcc atcatcatgc
     2461 gtgacaaaca atccagagcg ttctaacaat tcatttgaaa ttgattcatt cttgaatact
     2521 tgctgaagaa aagaacgctc attaggtgca aaacctatgt tgaattcttc aattaattct
     2581 aaagacaacc cacgactttg caaatattct aaagctggct gtccagcttt tgtatgcaat
     2641 agcatgtgat gatagatttc cgctgctttt tcatgagcag caatcagttt ccccgtagca
     2701 gattcaggat tttggacagc tatagtacgc tgacgccatt gatcttctaa cggtatctgt
     2761 tcgaagtctg ccacttttac cactgcttct ggaaaactta gaccttctag ttcttgaaga
     2821 aaagtaaaca cgtttccgcc tttaccacaa ccaaagcaat gaaaaatctg cttgtcttct
     2881 gcaacagaaa aagacggtgt acgttcttca tgaaaaggac acagccccaa gtaattttta
     2941 ccagactttt tcaactggac atattgtcca atcacctcaa cgatattcgt ttgactgcgt
     3001 actgtttcga taacctcttg aggaatccgt tgagccattt tttcaccccc tgctctattg
     3061 atatcttttg ggtttaacag aagatatttc aggagaataa ccggtaaaaa atcgcactaa
     3121 ctgaaatcag tgcgatttta aaggacacaa ttatacatgt ttgattgtat catatttcat
     3181 tgctttttca agtataacac atttctatct cattaagcat caatttgcta ctcttttcag
     3241 taactgattt tgaatatcag ccttccagta caatactcga agaacaatat ttacgagcat
     3301 gaatacaaag aagtatccta cgctctctcc caaccctaag tttacttcca aaattccatg
     3361 gaacagcatt gcagctacat atatccctac gatcgtcgtt actgctagga agatgcgcaa
     3421 tgggttgaag ggataacaag cttttacaac tgccatgcaa ctgatcccaa tcagcaagta
     3481 atacatcaaa gtagtagttt cagcatttcc ccatgcgaat gtttttccaa gtagatacac
     3541 cgcaatgata ttcactacta caagcaaggc atttggtaaa gcattaataa gtgctgtagg
     3601 caaataacgg taagtgattt ttcttttatc ttgttcaaaa gataaaaaga aggaaggata
     3661 cccttcaatc gctaaatcaa tcagcgtgat ttggataggg ataaacggaa atccaattgc
     3721 tgtaacggca catataatcg ataaaataaa tgaataaatc gttttgataa aaaagatgcc
     3781 tgagactttc gtcacattgt tgaccactcg gcgtccttca aacaggactt ctggcaatgt
     3841 agtaaaatca gagtccaaca ataccagatt cgcaatctga cgtgttgctc catcaccttc
     3901 tgccatagca atgctgcaat ctgcttctct taacgccaaa acatcattca caccgtcgcc
     3961 tgtcattgca accgttctgc cgctttcttt caattcattt accagcaatt tcttttgctg
     4021 cggactcact cgtccaaaaa ccgtatagcg atgagcagct tctcgcactt ccatttcttc
     4081 agtaatttga gaaagatcga catacgcatc ataattcggt aatcctgcac gtctagcgat
     4141 attggagaca gtaaaaggat tgtctccaga aatcactttc agatccacac cttcctcatg
     4201 aagataagct agtgtttctt gagcgttttt tcggattgga tcatcgattt caaacagagc
     4261 aaccggctca acaaatgctg gtttttcttc gttcaattgc tgatttggag cgatccccaa
     4321 cataagtacg cgatagccat tcgcttgggc tgtttttaga ctgtccggaa gccgatcttc
     4381 ttccaccaaa cgttcaggcg ctcctacgta cacgactccc agttctggaa attccatcgc
     4441 gccccatttg cgatcagaag aaaatgcgac tttgtttgtc acatcatacc gattagaaag
     4501 ctcaaaataa tctctcatag cccgcatagt gatgttgtta tcactgcttt ctgccagata
     4561 cgtccccata atttctggga tggctttctc gtacagcgga tgtagtgctt ctacccgctg
     4621 gatactcatt ttcccttcag taatggtccc tgttttatcc aagcataatg tatcgacatg
     4681 ggccagcgtt tcaattgaat acatgtcttg aacaaggatt cgtttttttg ccagtttggt
     4741 tacccctgtc gttaatgcaa tacttattag caataccaaa ccctttggaa gcatgcctaa
     4801 caaagctgct gcagatgcaa taacagaaat tttgatagaa gcatcgcgga tgaacagtgc
     4861 ttccaaaaat agtatgatcc ccaaaggaat aatcacgaaa ctagtgaatt tggatacttt
     4921 gcgaatcgaa tctacaagtt cagaacgaat agctttgtga gtctttgcct cgctagtcaa
     4981 gcggacagca taattatctg cacctactcg gataacttca gccatgacag aaccgcttgt
     5041 caaaaagctt ccagaaagca attcagctcc agtttctttg acaatcaaat cagattcgcc
     5101 tgtcaaaaga gcttcatttg cttctgcccg tccagatata acctgtaaat cggctggtat
     5161 ctgttctcct gctccgattt ttaccaaatc tcctaatacc agctctttag tagaaatcga
     5221 ttgttcttta ccgtcccgga tcactagtac ttctgattgg gaaacaatcg ataatttccg
     5281 tacaagattt cttgcatgta tttcttgata aattccaata acgatattga cgacaataat
     5341 tgctaaatac gccatgttgg aatacgcacc tacaaataac agacaaatcc caataccaaa
     5401 gttcagaaaa ttgaataacg tcaatatatt atcgctgaat atttgttttg tactttttga
     5461 tgcatcttcc tggtagtcat tttgctggcc agaagccatt ttctgaacta cctcttgctg
     5521 tgtaagtcca ttcattttct ttttctccat tttccgccaa ctttcaaaaa taatcacgtg
     5581 tttaagtcta gcgtgttcca agattaaaaa caacttgttt ttataaaatt tatagaaaaa
     5641 cacacgaatg attttacaaa aaagatgcca acctttttgt ttgaatcacc cgtgtgctgt
     5701 ttttattatc aatctatctt tacttatatt gttcctccaa acggctcatc caccaaccta
     5761 gtgctgtacc ggctataaaa agaacaggac aagctagtac cccagcaact cccaatcccg
     5821 cgtaatcggt aggaatgatc ataatcgttg aagcaaaaac tacacctaaa atgaaatgga
     5881 atagcttgct atatgccttt gagaaaatgt aatccatgat ttttgacaat ccaagaacac
     5941 aaagaatccc accaattccg atcggcacaa tcacttcaag tcgtacttct ttaaatccgt
     6001 cagccatcgc tttatacaat cccatataca ccaaaaaatt agaagggctc aatcctggga
     6061 cgatcatacc caaaccaatc aatccgccgg caatcagcca tgttccaaaa ttctgatcga
     6121 cttgcccgtc aaataattga gcgccgtata ataaaaacaa ataagccaga acaaaagtaa
     6181 caaccatgat gatcacatca gaagtatctc ttcctttttt ccctgcttct ttccataacg
     6241 caggaacagt tccagcgata catccgacaa aaaaccataa tatcgttgtt tcatacgcac
     6301 ctaataaaaa actgactaaa aaagaaagta gaaagatgcc tgtcactccg cctagaccaa
     6361 caggcagaaa aaacaaaaca ttttctttga aattttttgt aaggtgggct aaaaaagaaa
     6421 tcagccgctc atacatgcca aagacagctg ctaatgcacc accgctgact cctggcaaga
     6481 taaatcctga accgataaac attcccttca caaaccgtaa catccagtct ttggttgtgt
     6541 accccacttc ttgattttcc attttttgcc ttttttccac tatttgcgca ctccttcttt
     6601 catttaattt tattcatgat tgaacatttc ctgctatttt acttaaagac tttttcatag
     6661 gtctctatta ttttttcatt cattttgaca atatcaggat aaaacatgtt acctccatta
     6721 tgatagagat agccgccatt gtaaagcaag gactggatcc gccaatatcg gtattgtttc
     6781 ccttcctcat ttcctaaaag tgggctcagc acatctttag aatacgcttc tgctaattct
     6841 actgtgtttt ctccgccatg attttttaca taggcaatat aatctttccc aaaattatat
     6901 gcttggacag ctgtccataa atcgcatcct tgtttctttg cttctactat catttccgcc
     6961 aaatatttga caccttgatc aatactgtca gctggattat caatgatctc agcttctcca
     7021 tgcagacttt ccgagctctg catgagatct gtcccttttc cttttgattc ggtcaatatt
     7081 atcgccttga tcaaattctc gtagttttcc atatgataat tttttgcagc tgttgttatt
     7141 tctatttgat agctttccac tcgttttacc gaacgataag ttcttcctca taaaaacaac
     7201 accgcaccaa taaccgtcag aatgatcatg taaaaaagaa tacggataat agataatcgt
     7261 tttcgcttca tttcatcacc tagttatttt gacatctttt tttatcttac tatttcttta
     7321 tcttcttggc tatttcttcc ttgatttctg tgtacgcctt aagaaaaaat gaattttcct
     7381 gtattttttg acatcttacg aaaatgatcg ttgtttttca gtaaaaacaa ttgcccaatg
     7441 actgatataa tcgttatact ttacacagta ggtgattatg tgaaaaaaga aaagaaatat
     7501 gcaaaaatgc cagatgggag cgaaatttac tatgaaaaga gcggtcaagg cttccctctc
     7561 tttttactgc atggcaatga cggcagcggc cgcttttttt cagaacaggt ccctgtactc
     7621 gaacgctact ataccgtgta tctagtagat agccgaggac atggtcgttc gacaaacgaa
     7681 gcatctatgt tgaattttca gttaatggcc gaggatctga atacgatcat gttgctggaa
     7741 aaaatcgatc aggcagattt tcttggattt agtgatggag caaatctagc actggtattt
     7801 gccagcagct tccctaaaaa agttcatcga ttgattttaa attctggaaa cacgcttgtt
     7861 aaaggcgttc gtttttctgc cagagtcatc agtaatattc attatgcatg ggtatggtta
     7921 ctctctttat ttcgcccaag tcttcgcaaa aatcttttag ttatcaaact tttgctgcat
     7981 gacatcgggc ttacagaaaa tgatttaaaa aaaatcaact cccccacttt gatcatcgtg
     8041 ggaaaaaaag atgtaatcaa gctaaaacat tctttgtata tagctaaaac tataccaaaa
     8101 gcttcctttg tgcttgtgaa agaacaagga catgaattag ctagaaaaga tcctgaacgc
     8161 ttcaatcgag aagttcttca atttctttct gaaacataac tattaggaat agaaggtgat
     8221 ttatttgtta aaaaatacca tacaatggct gaaaaaccac ctaggactgt tcaaaacaat
     8281 ctttttgatt tctgtcattg tcattattgt cggtgaattg atgtccatcg gtaaaactct
     8341 atctatccag caattaggag aaacgtttgc cactatacct gtatggaaaa gcggactaat
     8401 gctattgatt ggtcttatct ctgtgttacc tatggttggt tatgatatta ttttgaatcg
     8461 gatgcttgaa caaaagcaga atcctcgtta cctgtttgag acaagctggc tgatcaatac
     8521 gatcaacaat attgccggtt tcggcgggtt tgttagtgtg gggcttcgct ctgaactata
     8581 tggacaaaag aaagagagca gaaacgtgat ccaagcatta tcgaaagttt tcttgttttt
     8641 gatggctgga ctttctattt acagtctgct ttctttttta cttgtagtct tcacgccagt
     8701 tgctcctttt ttgaagcaat attggatatg gttgatcggc ggcggtctat attttcctgt
     8761 cgtcttttta tttactactt tcaaaaagaa aggattcctt ggtggtacct cacttaaaac
     8821 tcaaggacag ctattgctag tttcattgtt ggaatggacc ggtgtcctgt ttagtttcct
     8881 ttctgttggt tatttaatgg aaatccggat tgatctgtgg caaacaattc cattattcat
     8941 tgctgcttca gtgataggaa tcgtgtctat gattccagga gaaattggga gtttcgatgt
     9001 aatgatgatt atcgggttgt ccgctattgg tgtgccaaga gaaatagtcg ttgtgtggat
     9061 cttgctctat cgcctgttct actacatcat tccgtttttg attggaatcg tctttttctt
     9121 caaaaatatc ggttccactt ttgatcagcg ttattctggt atccctaaac aattagcaac
     9181 agaaattgcg cataaaatcg tggttgtctt attatacttt tctggaatca tgctagtact
     9241 ttctgccact atcccgcaag cttttacaga gtttcgctgg cttcatagtt tgaacccatt
     9301 gaaatttcat tttattatcc aatttcctag tatactattg ggatttcttt taatcattat
     9361 gggcagaggg atcgcagcac gtgtgaaacg agcatatttg cctacaatct tcttgattgt
     9421 tctagcttta ctttatgttt tattaagtga ttttagtttc acaccggtgg ttttcttgtc
     9481 tattctgctt ctgatcattc ttgcttctaa aaacgaatta tttcgggaac aattgatcta
     9541 ttcatgggag tggcgtacga tcgatggaat cattattggt gcactgtcct tgctttacat
     9601 cgttattggg gtatacaatc tgccggattt ccctcatcgg cgtcaccatt ttatttcctt
     9661 tttcttattt ccttctgaaa agatttggtt ttcgggattg ttggccatca tcgctgttag
     9721 tttcatgatc gttctatttg ttcatttcct tcaaggagaa aagaaacaga ttggcgaagc
     9781 ctttaatgaa gaaaaggccc tgaagatcct tacaacttat ggaggaaact ccgatagtca
     9841 attgatcttc ttaaaagata agcggatgtt tgcctatgaa aaagacggtg aaccaactgt
     9901 tcttctccaa ttcgcttgct tcaataataa atgtattgta atgggtgatc cttctggtaa
     9961 gaaagaagat tttccagaag caatcgaagc ttttatcgaa gaaacagatc gactatgcta
    10021 tttaccagtt ttctacgaaa caagcgaaga gattgttatg attttgcatg aattcggcta
    10081 cgatttcatt aagatggggg aagaagctta tgtagacctg aatagtttta caacttcagg
    10141 gaaaaaaatg aagggcactc gtgctgtctt gaatcggatc gaacgagaag gatttacatt
    10201 tgatgtctta caaccaccat tttctactga gcaaatgagt actttcaaaa atatctctga
    10261 taattggttg ggttcacgaa aggaaaaagg gttttcttta ggctttttct ctgaagatta
    10321 tctacaaaga gcgcctattg cagttgtcaa aaatacccaa gaagaaattg tcgcttttgc
    10381 aacgatcatg cctacttaca cgaacaataa agtaggaacg attgatctga tgcgatatga
    10441 ttctaaaaaa gcaccttctg gcagtatgga tttcttgttc ttgcatttgt ttgcctatat
    10501 gaaagaagaa catatccaat ggttcaattt agggatggcc cctctttcaa atgtcggaac
    10561 ttccagaaag agttttttac aggaaaggat tgcatacttg gtttacgagt ttggttctca
    10621 tttttattct ttccatggat taagagaata taaaagcaag tacgctacaa actgggtctc
    10681 acgttatact ctatattcta gagatagttg gattgcatat gttatgattg ctttgctgat
    10741 tattgataat gcgccagttg agcagacaca aggtagaata agtggtttga aaaaatggat
    10801 acgaagaaag tattaaaaac tgcataccga tagatgaaca ctctcatgat gaataaaaga
    10861 gactgaagta aaacgaaagt tcatgcttct acaatctctt cttatacaat tgaagaacaa
    10921 agagcggtcc aaacagaaat cctgtttgga ccgctctttt attttaaaca tttttcatta
    10981 ccaaaatatt cgagtcgcgt ttcttatgct tcgatctctg taacgatacc tgaacctact
    11041 gtccgtccgc cttcacgaat tgagaatgtc gttccgtttt caacagcgat tggatggatc
    11101 aagtcaacat caatcgtcac gttgtcacca ggcatgacca tttctgtatc ttctggcaat
    11161 gtgattgttc ctgtaacatc agttgtgcgg aagtagaact gtggacgata gttgttgaag
    11221 aatggagtat gacgtccacc ttcttctttt gtcaacacgt acacttctgc tttgaatttt
    11281 gtatgtggag taatggaacc tggtttagca agtacttgtc cacgttcgat atcgtcacgt
    11341 tggatccccc gtaacaacac gcctacgtta tccccagctt ccccgtaatc taacgtttta
    11401 cggaacattt ctacacctgt tacaactgct ttttgtgttt caggtttgat ccctacgatc
    11461 tctacctcat caccgacacg aacagcacca cgatcaatac gacctgaggc aacagttcct
    11521 cgacctgtaa ttgagaagac gtcttccact ggcaatagta atggtttatc tgtatcacgt
    11581 tctggtgttg ggatatattc atctactgta tccatcaatt ccataatagc agcttcagca
    11641 tctggatcgc cttgcaatgc ttttaatgcg gaacctttga tcacaggagt gtcatcgcct
    11701 gggaaaccat attcgctcaa taactcgcga acttccattt ctaccaaatc gatcaattct
    11761 tcatcatcga ctaaatcaac tttgttcaag aaaacaatca ggtattttac accaacttgg
    11821 cgtgacaata aaatgtgttc acgtgtttga ggcatcggac cgtcagttgc agaaacaact
    11881 aagattgcac catccatttg tgctgcccct gtaatcatgt ttttcacata gtccgcgtgt
    11941 cctggtgcat caatatgcgc gtagtggcgt ttttctgttt catattctac gtgtgcggta
    12001 ttgatcgtaa tcccacgttc acgttcttct ggagctgcat caatactagc atagtcttga
    12061 ggatttgcta ggcctttttt gcctaatact gtagtgatcg cagcagttaa agtagttttc
    12121 ccatggtcaa cgtgtccaat cgtaccaatg ttaacgtgtg gtttacttct gtcataatgt
    12181 tcttttgcca taatttacaa acctcctaaa taatatagat cactgaaaaa tttcagctct
    12241 caccttttta ttatagtctt tttttgacta aatcaaaaag aatacgtccg acttactttt
    12301 tgtaagattc gaactaagct tattatacgt caatattggt caaagtcaac taagagcact
    12361 cgtctgtctt atacgcgaaa aaagtacctt ttataaataa aaaaatgtgt tttttgagaa
    12421 aaacttcaag atagtcctta actttctttt gaagcaataa gaacaaaatg aggaaaagtt
    12481 taaaattcag ctgttgccaa caactttcaa tagacgaaat cttctatttc gttaataata
    12541 aggaaagagg tgaagtacat gaaaaaatta attggcgtac ttgtcgtttt aggcatattt
    12601 ctcggcggaa gttttgcagc ttatcattat ttttacggcg gcgaagctta ttatacaaaa
    12661 atcacgacga atggcgaaat gtcagctacc caagcagata acggagagaa attcacgact
    12721 tatacctaca agcaagcggc ttataataaa gatggtgaaa aaaaacaggt gacgcttcgc
    12781 gaagaaagag aacatccatt aaagatgaat gcgtatctta aactcaaagt caatcctcgt
    12841 aaaggtgtat taagctggga agaaataaag gcttctgaag tgcctaaaaa tgcggcagaa
    12901 aaaatcgata attagtaaat ggtcttaaaa ttttattaga taaaa
//
LOCUS       DAZULO010000076        12734 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000076 DAZULO010000000
VERSION     DAZULO010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.078"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACG8OA_002112"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACG8OA_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS4976873.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ACG8OA_002113"
     CDS             complement(322..891)
                     /locus_tag="ACG8OA_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFS4976874.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ACG8OA_002114"
     CDS             1077..1979
                     /locus_tag="ACG8OA_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4976875.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACG8OA_002115"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACG8OA_002115"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFS4976876.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACG8OA_002116"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACG8OA_002116"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFS4976877.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACG8OA_002117"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACG8OA_002117"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFS4976878.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ACG8OA_002118"
     CDS             8547..8732
                     /locus_tag="ACG8OA_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFS4976879.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ACG8OA_002119"
     CDS             complement(8794..9990)
                     /locus_tag="ACG8OA_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS4976880.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="ACG8OA_002120"
     CDS             complement(10160..11596)
                     /locus_tag="ACG8OA_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS4976881.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="ACG8OA_002121"
     CDS             11889..12698
                     /locus_tag="ACG8OA_002121"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4976882.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DAZULO010000077        12701 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000077 DAZULO010000000
VERSION     DAZULO010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.079"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(287..649)
                     /locus_tag="ACG8OA_002122"
     CDS             complement(287..649)
                     /locus_tag="ACG8OA_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976883.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="ACG8OA_002123"
     CDS             complement(723..1103)
                     /locus_tag="ACG8OA_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFS4976884.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="ACG8OA_002124"
     CDS             1185..2360
                     /locus_tag="ACG8OA_002124"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFS4976885.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="ACG8OA_002125"
     CDS             complement(2437..3021)
                     /locus_tag="ACG8OA_002125"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4976886.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="ACG8OA_002126"
     CDS             3222..4220
                     /locus_tag="ACG8OA_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4976887.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="ACG8OA_002127"
     CDS             4332..4841
                     /locus_tag="ACG8OA_002127"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFS4976888.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="ACG8OA_002128"
     CDS             complement(4871..5530)
                     /locus_tag="ACG8OA_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFS4976889.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="ACG8OA_002129"
     CDS             complement(5532..6296)
                     /locus_tag="ACG8OA_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFS4976890.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="ACG8OA_002130"
     CDS             complement(6304..6981)
                     /locus_tag="ACG8OA_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFS4976891.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="ACG8OA_002131"
     CDS             complement(7018..8403)
                     /locus_tag="ACG8OA_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFS4976892.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="ACG8OA_002132"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8OA_002132"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="ACG8OA_002133"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="ACG8OA_002133"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFS4976893.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9342..10199
                     /locus_tag="ACG8OA_002134"
     CDS             9342..10199
                     /locus_tag="ACG8OA_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4976894.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10280..10615)
                     /locus_tag="ACG8OA_002135"
     CDS             complement(10280..10615)
                     /locus_tag="ACG8OA_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976895.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10682..12052)
                     /locus_tag="ACG8OA_002136"
     CDS             complement(10682..12052)
                     /locus_tag="ACG8OA_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFS4976896.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12130..12666)
                     /locus_tag="ACG8OA_002137"
     CDS             complement(12130..12666)
                     /locus_tag="ACG8OA_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4976897.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3773 a         2579 c         2119 g         4230 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac
     9361 ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt
     9421 cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa
     9481 ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc
     9541 gctatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc
     9601 acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat
     9661 ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa
     9721 taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg
     9781 attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg
     9841 tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc
     9901 acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa
     9961 aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga
    10021 tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc
    10081 ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc
    10141 taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa
    10201 gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt
    10261 gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta
    10321 catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt
    10381 tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga
    10441 tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc
    10501 gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta
    10561 cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta
    10621 tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca
    10681 ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc
    10741 caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt
    10801 gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta
    10861 tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt
    10921 tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta
    10981 actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat
    11041 gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac
    11101 ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg
    11161 caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac
    11221 cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct
    11281 caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg
    11341 caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag
    11401 acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta
    11461 ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc
    11521 gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg
    11581 cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga
    11641 tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac
    11701 caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc
    11761 caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact
    11821 ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc
    11881 tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt
    11941 ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa
    12001 gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt
    12061 ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca
    12121 tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc
    12181 gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg
    12241 caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt
    12301 gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga
    12361 ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac
    12421 aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat
    12481 ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta
    12541 tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc
    12601 ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc
    12661 catcattctt acctccgtct cttttcatct acccataaat a
//
LOCUS       DAZULO010000078        12476 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000078 DAZULO010000000
VERSION     DAZULO010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.080"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            31..504
                     /locus_tag="ACG8OA_002138"
     CDS             31..504
                     /locus_tag="ACG8OA_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFS4976898.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            513..740
                     /locus_tag="ACG8OA_002139"
     CDS             513..740
                     /locus_tag="ACG8OA_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4976899.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1376..1747
                     /locus_tag="ACG8OA_002140"
     CDS             1376..1747
                     /locus_tag="ACG8OA_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976900.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2003..2245)
                     /locus_tag="ACG8OA_002141"
     CDS             complement(2003..2245)
                     /locus_tag="ACG8OA_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4976901.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2277..3179)
                     /locus_tag="ACG8OA_002142"
     CDS             complement(2277..3179)
                     /locus_tag="ACG8OA_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4976902.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3192..3380)
                     /locus_tag="ACG8OA_002143"
     CDS             complement(3192..3380)
                     /locus_tag="ACG8OA_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS4976903.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3394..3957)
                     /gene="amaP"
                     /locus_tag="ACG8OA_002144"
     CDS             complement(3394..3957)
                     /gene="amaP"
                     /locus_tag="ACG8OA_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS4976904.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(3995..4888)
                     /locus_tag="ACG8OA_002145"
     CDS             complement(3995..4888)
                     /locus_tag="ACG8OA_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4976905.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4966..5889)
                     /locus_tag="ACG8OA_002146"
     CDS             complement(4966..5889)
                     /locus_tag="ACG8OA_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS4976906.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5938..6288)
                     /locus_tag="ACG8OA_002147"
     CDS             complement(5938..6288)
                     /locus_tag="ACG8OA_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS4976907.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6321..7223)
                     /locus_tag="ACG8OA_002148"
     CDS             complement(6321..7223)
                     /locus_tag="ACG8OA_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS4976908.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7216..8073)
                     /locus_tag="ACG8OA_002149"
     CDS             complement(7216..8073)
                     /locus_tag="ACG8OA_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS4976909.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8407..9216
                     /locus_tag="ACG8OA_002150"
     CDS             8407..9216
                     /locus_tag="ACG8OA_002150"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4976910.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9256..9753
                     /locus_tag="ACG8OA_002151"
     CDS             9256..9753
                     /locus_tag="ACG8OA_002151"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFS4976911.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(10138..10211)
                     /locus_tag="ACG8OA_002152"
     tRNA            complement(10138..10211)
                     /locus_tag="ACG8OA_002152"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10175..10177),aa:Ile,seq:cat)
     gene            10398..10721
                     /locus_tag="ACG8OA_002153"
     CDS             10398..10721
                     /locus_tag="ACG8OA_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976912.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            10885..11139
                     /locus_tag="ACG8OA_002154"
     CDS             10885..11139
                     /locus_tag="ACG8OA_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976913.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            11209..11454
                     /locus_tag="ACG8OA_002155"
     CDS             11209..11454
                     /locus_tag="ACG8OA_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4976914.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            11530..11895
                     /locus_tag="ACG8OA_002156"
     CDS             11530..11895
                     /locus_tag="ACG8OA_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFS4976915.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(11956..>12476)
                     /locus_tag="ACG8OA_002157"
     CDS             complement(11956..>12476)
                     /locus_tag="ACG8OA_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976916.1"
                     /translation="EYLEIINFRLKTKKESVIILVYFQGGNLMIKKLFLLSILTLAMA
                     GCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCSNRRRC
                     RRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAFSFARE
                     DEDNPKTYDILA"
BASE COUNT         3593 a         2328 c         2133 g         4422 t
ORIGIN      
        1 ttagctatca aagataaaag agggataaaa atgaaattta aaacattgct attaaaattt
       61 gtcgttttag gtataagtgc cgtatttatt ttgttcggcg tactttttta tactcagatc
      121 gctacaagcg aaaaaggatt caattttgat tggcagacca cattatttgt ttctgtcatt
      181 tactttgccc ttctcctagg gatcgttatt gcttatttct tattgaaact attgaaaaaa
      241 atcgaagata accaagcttt ttccactcaa agcttaaaca tactaaaaaa aatcgaacga
      301 accattttgt ttattggttt cgcctttttt ggcttacttc ctaaatttat gaaagtagcg
      361 gatttagacg atgctccagg tttgatgttg gtaggaatcg ctgtcgtatt cattcctttt
      421 gcagtataca cattaacaag cgtgctggaa aaactgcttg aacatgcgat tttacttaag
      481 acagatcatg atttgacagt gtgaggtgcg acatggcaat tatcgttaat ttagatgtag
      541 tattagctca aaaaaaaatg agtgtgactg aattgagtga gaaagtgggc attacgatgg
      601 caaacatctc tattctaaaa aatggaaaag caaaagccat ccgtttttcc actctcgaaa
      661 aaatctgcca agttttggag tgtcaaccag gtgatttgtt gacctataaa aatgactcga
      721 atactatacc agaagaataa aataaagtag aacaaacagt aaataaaaaa taatctgaat
      781 gatagatgtg tgagactttt agaagttatt tacgtctatt attcagattt tttatttcga
      841 ttgaataccg ttttatctat aaataagata tgtactgact catacaaagg gggccttttc
      901 ttctccatgt actatcactt ttcctgatac aactggtttc ctgttacgtt gtagttggat
      961 ctgcttttag tataatactt tattcattcc accagactga tagcagtttg agcagtctaa
     1021 ataatcatgt agtattacag gccattctgt ttcatacctt gaagtgctta aaacttcagc
     1081 taatggccca ttgtgttaac tcttactcca ttctttttta gtaagctccc atatatactg
     1141 taaagccttc tatatattat gtcatgttgc tttttaacat ttactatgta gttttgataa
     1201 aatgatttga ataacagtga tggacaattc ttttttattt ttaacattat tttgataata
     1261 atgattattt atttaaaaat acattagata acatttgtta cgacacgaaa aagcttcaca
     1321 aacaaatatc gttattttaa aatgagggcg aactcacaca attaggagga atagtatgcc
     1381 atttattgtt tacttttttc tttcattact cgtgatctat attccattac caacattttt
     1441 cctgctcgtc aatcgtctag cagagcaaca tgatacgatt gctgctctta cgaaactttg
     1501 tttacttcta tttgtttgtc tagattactt tacgagctat tattggctaa aacagacaag
     1561 agtaaaaaag agatattatt tcttccttgt ctttttcaat ctctttgagt ttctgatcca
     1621 ttgcttttta cttcatcagt atcatacgat ccatttgtgg atcatctggg ttttcaaagt
     1681 ggtcgtatta ttttgtatgt tcacctttcc tatctcaaga aaatttaggg aggcattgtt
     1741 ttattaaaga agcttctctg tatcaataaa gacacattta aggaaaaact tttaaaaatc
     1801 taattgggaa tttgcccgca taaataagag gctgtgacaa aagtcttgtc acagcctctt
     1861 attccgtata aacagtgttc aaaagcttgc atcttcgata atatgcccaa ccaaatgaaa
     1921 aatatgagaa gctatttttg tttggctgtt cttattactt gatgaagaac agctttttca
     1981 cgacctcttt ttaaaagtct ttttacagtt ttcgagcaaa gaatgatacg attagtacta
     2041 aaatgatggc accaatcaat gatggcacga ttgccattcc tgcaatactt ggtccccaag
     2101 aacctaataa tgcttgacca agtgctgatc caactaaacc tgctacgaca tttgcaataa
     2161 tccccatcga agaaccttta ttcgtgattg ctcctgcaat tgctccgata attcctccga
     2221 cgataagtga ccaaataaat cccatgtttc gttcctccta atgttttact tccttattac
     2281 tgaacccgtg gttctgtatt ttcttttact ttttgtgcgc cgcttccagc tgctgatttt
     2341 actttatctg tttgattcga agcaaattca ccggtttttt tcgctgcgtc agttactttg
     2401 tcttgcaccg tttcttgatc tttttcaaat tcttcttttg ttttgatgtc cactacgtta
     2461 gcgttgactt cgatcacttc taaatgtgtc atatttttta cttctttgcc gattacttct
     2521 tgcatttctt caaagatttt ttcgatatct ttgccatatt ccacaatgac atccatatca
     2581 actgctactt gttttttacc gacttctgtt tcgatcccag ccgttacatt atctgtattc
     2641 acaagttttc ctgccacatt agagaagaaa ccaccgtttg cagataataa accgtctact
     2701 tgttcaattg cgattccgat gattttttgc acaactttat cttcataatt cagctcaccg
     2761 cgcattgaat cttcctgttc agaatcatgc tttccttggt tatttgtatg atcagaacca
     2821 agttttcctt gtccgtttgt gtggtcagaa ctaagttttt cttgtgcttt cgcatggtca
     2881 gaaggatgtt tttgctcttc cacaggttta gaaggatcga ttgcatcatg ttgttctgga
     2941 actttagatt ctggagttcc tggatgatga ggtgcatgct tttctgcatc atgtttcttc
     3001 tcttctactc cttgttgaac gttagggact actggatctg gatgtgcaga tactggtgct
     3061 gttttgttct tttcatcgtt cacaccattt gattggtttg gtgtcgttgg gtctggatgt
     3121 gcagacactg gtttttggtt gttcatttta ttgttattat ttttgttatt gttattcatg
     3181 gatgtttcct cctaagattt aaaatagtga tctaagattt tcgtttgttt cagataaaaa
     3241 ccgatagcga cgccgatcgc tgccatgatc aagatcaata aggtcttgaa aaagcccacg
     3301 gtgatccata aaacggctaa tatcaatcca agtaccccaa agatgatagg gagcttgtat
     3361 gtcgaaaata gttctttcat ttttggtccc cccctattct actcgtttgc ggtcattgct
     3421 tcgtttagct ggttcttcaa agcctgtgta tttcaccgat actttcactg gctcttttgt
     3481 tcccaatatg tgttgtactt cattttcgat ttctttcatc aatgttcctg ttttaccaat
     3541 gagtgctgaa gttcgtttca actccccttt tacattgacg gcgatcttgt ttttcgttgc
     3601 tttgacattt acttttggtg aatggatgaa ttcatcttca tgcaagtgag aacgaaccat
     3661 tccttcaatt gcttttttat ccaacgtcaa ctccccatca ccatgtttca gtagaaagtt
     3721 tccgattgtt tttggataaa acaggaaaac aagtaacgtg ataattgtca aaatagcaaa
     3781 tgcaacggca agccaaaata catattgttg cacgtatgaa ttagtgatgg aaaagaatcg
     3841 aaacttctct agctgaaaag gcaatggata ggttgcactt aatagtatcg caaacaaaag
     3901 cacagatagc aaaagtaatg ttccgatcac tccgatcgtt tttacacctt tattcatttt
     3961 cgcacctcct cataggggtg aaacgatcaa cttcttataa gttgatcgct tctccaccag
     4021 ttactccgta aatttgggct gtaacataac ttgctttatt cgatgctaag aaaacataaa
     4081 ctggtgctaa ttctcctggc tgacctgcac gttctagtaa actttgctga ccaaattcag
     4141 ggatcgaatc agaaggttgt ccttcgtcta gttgtaatgg tgtccagatt ggaccaggtg
     4201 ctacaccatt tacacggatt cctttaggtg caagctgttt tgctaaaccg atcgtaaagt
     4261 tagcgattga agcttttgtt gctgcgtaat ctaataaatg ttcgcttgga ttgaatgctt
     4321 gaacagaagt tgttgttacg attgctcctc ctgctgggat atgcggaaca gcttctttca
     4381 ctaaagcaaa catagcgatg atgtttatca taaatgtgtc tttgacttgc tcaagtggta
     4441 actcagcaat agactgacaa gaaatttgct gtcctgcgtt tagtaccatc gtatctagcc
     4501 cgccaagttc actcacggct ttatctatga tttctttagg tgcttgttcg tcacgaagat
     4561 caaaaggtag taagacaatc ttacgtcctg catctttcac gtattttgct acttcctctg
     4621 catctttttc ttctcctggg aagaattgaa gagcgatatc tgccccttct ctagcaaatg
     4681 caatcgctgc tgcacgaccg atccctgaat ctccaccagt gattagtaca cggcggtttt
     4741 ctagttgatt attgccttca taggattctt ctccacaatc aggaacagga atcatattct
     4801 gctgtaaagc tggtgtatcc tgatcttgtt taggaaattt ctccgtatgg tacaagtttc
     4861 gcgggtcgat taattttggt tctgtcattg ctgtattcct cctagttttt aaattgtatt
     4921 gccaaaacaa atttctttgt ttgatacctg ttttaaatag attctttaat ttgtataatc
     4981 tttccggtat aaatggtaac ctaaaactac tgctgctatc aaagagccaa agactacgag
     5041 tataaagcct agagcagaac ttttacccgg taatcctcct gtattcattc cccaaatccc
     5101 tgcgaacaac gccggaacgg aaatgattaa tgcagctgaa tctagatatt tcatgatatg
     5161 atttagatta ttgtccatca tcccgttgaa tagatcacca atactctcta ataaatctcg
     5221 atagatctcg atcatttttt ctgtttgtcg ctgtctcaaa tggacatcat agagtagctg
     5281 ttcatcgtct aaacgtttga tgaaagtttc agaattccat aattgatcca ttgtttcccg
     5341 ttgatctctt aaggtatgat cgatatatac gatgtctctt tcaagatctg cctgtttata
     5401 cagttcttca ttttccgttg ttttacgagc tgctttatcc aaagcgtcaa ttcgcttttt
     5461 catctctctt agttctttca cgtaatgggt gtaaatatct aataatgtat acgtgatgat
     5521 cttttcaaac gagtttaaat gatttccatg ctttgtcagc agccgatcca caaattcggt
     5581 ttttctatcg atacaagtaa tcaagagatt atcggaaagt acaaaagata ttggcgttaa
     5641 ttttttttcg atacgattat ctgactgatc tagattaatc actaccaaag aaaatggatg
     5701 ctctagtttc gtctcataaa ggcgctccat ccgagaaact tcttccggat attcggttcc
     5761 tacgaaaata tcatcaggaa gctcgaattc ttcgattgct tgctcaatct cttcgggctt
     5821 tgcactatca agtacgagcc aattatattt tttttcatct gtttctatta cattcttttc
     5881 ttcatccaca aagtagtatc tcaatgtata cctccttttt ggactttctc gaaactatta
     5941 gctgaagaag cagttcttta atttgatatt ataatcgagt actgcgattt cattttctaa
     6001 tgaaaagctg tcaagctgtt tgttgaagtc catgttcaat cgtttaccga aataattttg
     6061 ctctaattta gatgtataga tgtccaataa tggatgctcg attttagtcg catattctcc
     6121 aaattccgaa acaaaaggaa tgatttgaaa aaagctgcct tttgcaaaag cacctttgtc
     6181 ccttagaaat ggatcggaat cattgataag cgcgagtgcg atttttgtat tttccccaac
     6241 cttttcaatt aacgtctcta ctgcattttt agacattgta atttccattc ctactccccc
     6301 tcttcagttg ttgtcttcta ttatttaaca actacatctg tttttggttg aacttttgca
     6361 tattttggat atttagggaa tttagctaca caacggaaag ccgtcatccc tgcatatttt
     6421 tcgtaatatt tcttcgttgg cccttcaaaa tccttgctcc acggtttcac atgtcctgaa
     6481 aaatggataa tgtaaggatt gttgcgagtt tcttcgtatt cacgttcacc tgctgctgtt
     6541 ggatgttttt ttgcttttgc catgatatag ccttgtgcat tccaacgcgg atgaagtggt
     6601 aaccaacggt catgtaagat agcatttaat gcatcttggt catggaatcg taatttttct
     6661 ggattatgtt cgatgaaatc taatactttt tgcgtaatat tttcatctaa ccattttttc
     6721 acattgatca gcatcaaacc tgagttgaaa taacgcattg atttagctga aattttcatt
     6781 ttttccagtc gttgatggaa cccagcgtct tcaacagctg ctacgattga atcaccgaag
     6841 tcaagacgcc ataatttaga gatatcttgt aaagcgatca tgtcgcaatc catatataag
     6901 atacgttcaa cttctgtgcc gcggaacaat tccggaatcg caatacgata ataagctgtt
     6961 tctggaatac gatcgctgat taccacattt gtaaagaatt ctttattgat tttcaagaat
     7021 tccacatctg agttcattcg tgcgtttttc actgagaagc gtaaaagctg tttactttca
     7081 aaatcgatat catcgtcaat cacatagaac cgaacaaatt tagacgggtt gcaattatct
     7141 aatactgaga caaataatgc agctaaatgt ggtacgaatt ttgtgttaca acttgcgact
     7201 actgctattt cttttttatt cattgatctc gcctactttc gttagagtac tgtgtgctaa
     7261 tttttttaag tataaattgg tatacggatg atctttcaac gtattccaag gtttatcatg
     7321 tcctgtaaag tgaacgatcg aaggtttttc atttccttct ttatactgac gttcatattt
     7381 ttcatttggt gctggatgac gttcaaaaat cagcgaagtc tgcatgttcc attttggatg
     7441 aagttgttcc caatcttcat aaagtacagc attcaaagca tcttggtcgt gatagatgat
     7501 tcgatcgcca ttttcactga ggtaatggat agttttttct gtaatttctt ttttattcca
     7561 ttgatcaatg tcaatcacca tcactcctga attgaagtac aagtcatcgg aatcgattcc
     7621 taatctttcc aaagctttca cttggcctgg atcgataata gcgccgattg tttttccatt
     7681 taacggctca tcatatagtt ttacgatatc gtctagtact aacacatctg aatctaagta
     7741 aaggactttc ttataatcct ctttagctaa taagtttggt aaagaaattc ttaagtaagc
     7801 ggtagttgtg atatggtcac tgactaggaa gtcttcgtag atatcttttt ccactgtcag
     7861 gaattggata cttgcattac ttgaatattt atttaccgtt tcttccaatc cttctttact
     7921 atattctgat agaccatcgt caatcacata aaattttatg cgtcttgttt tattgcaatt
     7981 ttctaatgca gttgcgatca tcacgctcaa gtaaggagca taattttcgt ctgaagcagt
     8041 taccacagga actacgccgt atctcttctc catcaaaatc actccttttt ctaatctttt
     8101 tctaacctgt tctacatcta gaatattcct catttggttt taaagcaaaa tatatgcttt
     8161 gttttcattg aatattttaa aattttttta tcacaaaacg catcggtaag cgctaactat
     8221 tcgtttttca tggcttttta ttcaaaggta ttgaccaaaa actactatat atagccacct
     8281 ttttcttaag aatgtttttt gaaaaaattc ataatgacaa agcaagttag tttcattttt
     8341 ttaaattctc atttaatctt aaggtaacag gaaaatagat atgtaaaaag aaagtaggaa
     8401 gataaaatga aactagcagc gattgattta gacggtacct tgcttgatcg aaatggaaag
     8461 ataccagaag aaaatatcca agcacttcgg aaatttgatg aaaatggcgg aatcgtaacc
     8521 gtcgctaccg gaaggaacag catttctgct aaagatattt tttccgaatt aggaatcggt
     8581 ggctatttga tttcttccaa tggttcactg attgctgaaa tgaaggatgg aaaaatggcg
     8641 aaagttctca gacgttcaaa aatagatagc gatacactga gaaaagcctt cttctatgct
     8701 caagaagcga aaatatccat cattgctagc cgagaaaccc aagatgatca gctgacattc
     8761 gatgaatcaa atctagtcaa agatgaccct tattatgaac atttcaattt acagaaccat
     8821 tcttttgatc aaattttaaa tatgttggaa gatccttctc ttagctatct taaattagca
     8881 ttaacagata agagtgaaga aaaactacag aagctacaaa gagatctgca agcagatggt
     8941 atccaatcag tattctctga tccgcatttc ttagaaatca cgcctacagg tatcactaaa
     9001 gctcattcac ttgtttttct ggcggattac cttggaatcg aatcgagtga catcatggct
     9061 tttggcgatc aagaaaacga cattgctatg ttgaattaca gcgggttgag tattgcgatg
     9121 gggaatgcac agcgacaagt aaaagaaatt gcaaaccaag taacactgac gaatgaagaa
     9181 gctggtgttg cttatatttt aaaaaacttc tattaaaaag aataaaatga cgatatgaat
     9241 gtaggaggaa agaaaatgtt agttattatt gatatgcaaa atcatattct tgatccaaac
     9301 agcgaatttt atattccaga gtcagatcaa ttggcagaac gtattgctca aagattagct
     9361 aaagcacgcc aaaataatga atatattctc ttcacacggg atataccagt tgaactgaag
     9421 gacaaagaag aagaacgaga agatttgcaa ttgatccctc ttctttctcc acaagaaaat
     9481 gaacgagtga ttaaaaagta ttactttacg attccacctg aaactttagc tgaaatccag
     9541 gaaactttat ttgaaagcaa agaagaacaa aaagagatcg aagtagtcgg gattgaaaca
     9601 aacttgtgtg tactctctaa tctgataggc ctgcagagtg cttttcctga agcagacttt
     9661 tttgttgatc caatgttagt ctccagtaga aaacacggag aatccgcttt agagctgttg
     9721 aaagatttca acgtctctat cgtcgaatca taaaaatatc tatcttcatt tacgatattt
     9781 aacacacaat aaaaaaagca cgcagaactg ttttagtcct acgtgctttt tctatcaaag
     9841 tagattggct tttttatgac acagagtgaa acggcaggct cacgacctct ttcttaaatg
     9901 gtgttcaatc agctgaccct cggtattagc ttaacaaacg gcaaaatatg aggaactatt
     9961 tttccgtttg ttaagctaat actcaggtca aaacgctgat ttcacaacct cttccttaaa
    10021 cggtattcaa aagcttgcat ctgcagtaat aagaacagcc aagtaaaaac ataagaagct
    10081 gtttttactt ggctgttctt attacttgat gcagggcagc tttttcacaa cctcttatgt
    10141 accttgtagg actcgaacct acgaccggac ggttatgagc cgtctgctct aaccaactga
    10201 gctaaaggta catcataaat aaaatttatt tttgttactt ttcaactata gcacgaatga
    10261 caaactctca caagtttcaa aatataagta aaataatatt gttattctta tttcttttac
    10321 ttgtgtcata actattttgt tactttaatt tttatttgct tattttagaa aaaatgttat
    10381 tcttttattt attctcaatg ttaggagtga ttattttgcc atttattgcg attttattcg
    10441 atttagttgc ggcagcagct tattttctcc aattaaatta tcaaacctca ggaattctgt
    10501 ttattggaat gatttttcaa ggtgtgatta cccttttgct tcttgtcatg atgctcagct
    10561 ataaaggaaa aagatatgcc cgtattcaaa caaaagtatt tgttaaatat gtttccattc
    10621 gctatgggat catcattctt tcctttttag tgaatgcgat cgtcctcttt ttatatgtat
    10681 tgaattactt agatatcaat ccattgattt tttctcgata gataagacac attcagcgga
    10741 acagttcctg cttatcagca gaaaactgtt ctgctttttc atttttcatt gatctattct
    10801 tcttttattt tcctagcatt tcatttgtga tgtatgcaat aacatgttat cttttattat
    10861 atgaatcgtt gaggagtccg ttacatgatt acgttggtga gagttttgtt ttgggtgcct
    10921 gcggtcgcac tggtcgcaag tattgtttat cttatgaatt ggaacaaaga acggttttat
    10981 ttagctattt tgactcttcc ggctatctac tttatgtgga aagtttttaa ttacaactac
    11041 tttgaacccg atagtgtttt tatagaagaa ttatcaggac tggttttgtc tttattgatc
    11101 gtcattcttt atttgatacg tttaaataaa aaacattgag tactattgat tgcatgtgta
    11161 gctttctata gtcattataa ctattctact ataatgaaag gtgattaaat gagtatcgaa
    11221 gaagctattt tactggatac tttacgttgt cgtctgaatt tcttaagaac tggccgatct
    11281 ttatctcaag tagctgtttc tagcgcgtta agtattcctt tattcaaata tcgtaagtac
    11341 gaagaaggag aagaaattcc aagcatcatt gaattaatct atatcgctga cttttacgat
    11401 atttccgttg attacctaat tggtcgctca gaaacagcaa gcaaactgaa gtgatttttt
    11461 ccagccttgg tgctggtttt ttcatttaac gtctaaggtt aacaatctat atataaagga
    11521 gacatttcta tgcgattatg gcatgaaaaa ttgatttcaa agcttcctcg tcaacagctt
    11581 ttaggacaac atcgggaatg ctgtgcatta agaggaaatg gctggggaaa gaaacatgct
    11641 actgtggatt atgtttttca ctattctcct tatagattat tccaatatca tcagcttgtc
    11701 atggatgaaa tggaaaaacg tggttacagc ccagcaccag agtggaaaaa tcctatgtat
    11761 cgtggcaaat catgcgaagc attttttgat ttacctactg tagagcgcaa cgtgccgatt
    11821 tatccggaac acgatgaagt ttatctagca gaatgtttag caaatttgga gcaaaaagga
    11881 atttatctta cctaatataa ctagaaaagc cttctatctc ctcttacttc attaaaaagc
    11941 tttaaccatc ttagatcaag ctaaaatgtc gtaagtttta gggttatctt catcctcacg
    12001 tgcaaaacta aaagcgtact ctagttttgg ttcatcgcca acttccctag acataaatga
    12061 acatctcctg acgtggttta aaagtaccgc atcttcgacg tctgtatacg atgcacagca
    12121 cagattgctt cttttgatct ttcttttgat ttttctgttg cttcttctac accttcttcg
    12181 attactacat cgatgcttac aattgcttct gcctaatatt cccatgcatc gtcgatttaa
    12241 gcctctgtag tcactgtgat cgtttccaca ttttaactcc tctgtcgtgg cttcagttga
    12301 ttttgtgatc agttcagttg ctaagccaga gtcagaacaa cctgccattg ctagcgttaa
    12361 tattgatagt aaaaatagtt tttttatcat aaggttcccc ccctgaaaat agactaaaat
    12421 aataacgctt tctttttttg ttttcagacg aaaattaata atttctaaat attctt
//
LOCUS       DAZULO010000079        12325 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000079 DAZULO010000000
VERSION     DAZULO010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.081"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..344)
                     /locus_tag="ACG8OA_002158"
     CDS             complement(<1..344)
                     /locus_tag="ACG8OA_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4976917.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREI"
     gene            complement(341..994)
                     /locus_tag="ACG8OA_002159"
     CDS             complement(341..994)
                     /locus_tag="ACG8OA_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4976918.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1282..1802
                     /locus_tag="ACG8OA_002160"
                     /pseudo
     CDS             1282..1802
                     /locus_tag="ACG8OA_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(1800..2519)
                     /locus_tag="ACG8OA_002161"
                     /pseudo
     CDS             complement(1800..2519)
                     /locus_tag="ACG8OA_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(2544..2729)
                     /locus_tag="ACG8OA_002162"
     CDS             complement(2544..2729)
                     /locus_tag="ACG8OA_002162"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFS4976919.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            2956..4229
                     /locus_tag="ACG8OA_002163"
                     /pseudo
     CDS             2956..4229
                     /locus_tag="ACG8OA_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4336..5367)
                     /locus_tag="ACG8OA_002164"
     CDS             complement(4336..5367)
                     /locus_tag="ACG8OA_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4976920.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(5374..6204)
                     /locus_tag="ACG8OA_002165"
     CDS             complement(5374..6204)
                     /locus_tag="ACG8OA_002165"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4976921.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(6201..7016)
                     /locus_tag="ACG8OA_002166"
     CDS             complement(6201..7016)
                     /locus_tag="ACG8OA_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4976922.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(7013..7837)
                     /locus_tag="ACG8OA_002167"
     CDS             complement(7013..7837)
                     /locus_tag="ACG8OA_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFS4976923.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(7827..8927)
                     /locus_tag="ACG8OA_002168"
     CDS             complement(7827..8927)
                     /locus_tag="ACG8OA_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4976924.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            9105..9890
                     /locus_tag="ACG8OA_002169"
     CDS             9105..9890
                     /locus_tag="ACG8OA_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFS4976925.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            9923..10306
                     /locus_tag="ACG8OA_002170"
     CDS             9923..10306
                     /locus_tag="ACG8OA_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFS4976926.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            10382..10513
                     /locus_tag="ACG8OA_002171"
     CDS             10382..10513
                     /locus_tag="ACG8OA_002171"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4976927.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            10482..10718
                     /locus_tag="ACG8OA_002172"
     CDS             10482..10718
                     /locus_tag="ACG8OA_002172"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4976928.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            10730..10816
                     /locus_tag="ACG8OA_002173"
                     /pseudo
     CDS             10730..10816
                     /locus_tag="ACG8OA_002173"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(10796..11767)
                     /locus_tag="ACG8OA_002174"
     CDS             complement(10796..11767)
                     /locus_tag="ACG8OA_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFS4976929.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3698 a         2776 c         2228 g         3623 t
ORIGIN      
        1 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
       61 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
      121 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
      181 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
      241 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
      301 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
      361 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
      421 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
      481 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
      541 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
      601 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
      661 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
      721 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
      781 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
      841 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
      901 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
      961 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     1021 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     1081 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     1141 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     1201 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     1261 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     1321 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     1381 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     1441 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     1501 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     1561 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     1621 attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc
     1681 ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac
     1741 ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt
     1801 tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca
     1861 tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct
     1921 tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc
     1981 agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct
     2041 cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg
     2101 taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac
     2161 aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta
     2221 aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg
     2281 gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac
     2341 aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa
     2401 tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca
     2461 gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc
     2521 cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc
     2581 aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc
     2641 ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca
     2701 tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa
     2761 gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt
     2821 gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat cagtcctttt
     2881 gagcataaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca caacacaatc
     2941 aaaggagcgt tctgtatgcc tgatcttcat cttatcaaac tattactagg aatcaaagat
     3001 ggaaacattc aaatcactca tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt
     3061 gtttctgcca cgctcattaa aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa
     3121 catgggctga tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg
     3181 aatcaacgga cttatcttgc tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc
     3241 tattttacgg cgaataccta cattgttgaa aaaaactgtt ttatcgccaa acaagtacat
     3301 tataaaatat tggaggaatt gactgaaaaa caagcaatga cgacgatttc taaacattgc
     3361 ggcgtttctt ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg
     3421 aaaataaatt ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa
     3481 gtcggtccct attctttttc ttgtatggat ggcgatactg ggaaattact ggatattctt
     3541 ccttcacgta agaagaaaga tctcatttct tattttatgc aatttgaacg acgtgcacgt
     3601 ttaaacgtaa aaatactcgt aacagatatg aacgcctctt atgctttgct gataaaagag
     3661 tgctttcctc atgccaaact cgttgtggat cgctttcaca tcgtcaagca cctcattcgc
     3721 agttttaagg atatttgact atgagtcatg aaaagcttcg accgaaacga tccaatacaa
     3781 gccaaacact atcgacaagt gaaggcttta tctcgacttc tgatcaaacg acaggacacg
     3841 cttgtttatg ataaatggac aaaatggcgt aattttggtt gggcctattt aaccgaaagt
     3901 gaagtcgtag aacgtctact ttctacttct gatgaactac gaatagctta tgcttactat
     3961 caagaaattc ttcaagcttt ttatgacaaa gaagcggaca tcttctttca gcttgtaaaa
     4021 acaatgccca agtcggttcc aagagaactg catcatataa aaaaagcgtt tataaactat
     4081 gaatccggca ttcgtttagc cttagagttg ccttactcaa atggcaaaat agaaaatctg
     4141 catacccaca tcaaagcatt gaaacgaata gcttacgggt taggatgaga accagaattt
     4201 tcttaatcaa tcatctgatc acttattaaa cataaaaaaa catctaaagc agaaaatcaa
     4261 ttctacctta gatgttctta tatcactcta tcagtcctat ttgacaaaga gccagaaatg
     4321 tttgccgact atacactagc tttgttgtgc aaaatgctga gtatcaattt ccacagggat
     4381 tgctagaata tctttcagct gaatgtataa ggcatcgcta attttctctt cccgatcgtt
     4441 caaccgatcc actaaaattt ctttgccctc cagcgtttga aaaacatacc gttgaatgtt
     4501 gcctaaaata aatgattcct tccatagaac cggaatctta accccctgag gaaccggttc
     4561 aaattgaatc agctccggtc ttaaataata aatgcagcga ttgcccttag cggaaaactc
     4621 gtgggtatac tgattcagct cataccattc gaaacgatta tagttgccga taaattccgc
     4681 aacaaatcgg ctctgaggat tctgatacaa ttccttcgag gtggaaacct gagccacccg
     4741 cccagcttcc atcacaaaaa tccgatcact gattcgcatc gcctcttctt ggtcatgagt
     4801 cacaaaaatc atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc gctgcagctg
     4861 ttttctaatt cgggcatcca aagcacttaa tggctcatcc agcagcagga ctttcggttc
     4921 agtgaccaat gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa gaaactggac
     4981 atttttatct gcctgctccg tcatatccac caatgatagc atctcaaaaa ccttttcctg
     5041 aatcacctca ggactcagct tctttacctt caaaccaaag gctacatttt caaaaacagt
     5101 catagttgga aacaatgcat actgttgaaa aacaaagccg atattgcgat ccttcgtagg
     5161 cttatcggca atattctcat tgtccagcac aatctcaccg ctctttggtt gttccaatcc
     5221 cgtgatacag cgcagcaacg tagactttcc gcacccgctg ggacctagta aagtaaccag
     5281 ctcacccttt tcaatctgaa tttcatcaat agctaaaatc tgtttttcat caaaaaacag
     5341 attgagatca tgtatttcta aaaacatttt ccgtcacaac tcctttaagg gcttcttttt
     5401 cgactgcttc tcttccctcg tctgcttaag ggacagctgt tgcttttgtc gataagtcag
     5461 cataaaggta gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca tcacagcgct
     5521 agccaaatgg ccgttttcgt tcatccgccg cagcatatag atgcgtaccg tctgaaatcg
     5581 accgccaatc aacaggttcg tcagcatgta ctccccaaaa accgaagaaa aaatcatcag
     5641 agaaactagg gtcacaccga tacggatgtt tggaaacagc acctggataa taatcgtaga
     5701 catcggggcg cccaaggttt ctgctgcatc cactaattcc ttcagattaa ttaagcgcat
     5761 ctggttgtta agactaagat agacaatcgg caacgccgta ataaacaatg cacctaccaa
     5821 cactacaaac attgggatac cagtttcagc atacgttcga agcaaagccg ttaccaaaat
     5881 cactcctggt aaagcatacg gcagcaggag cagcttttcc agccaacgat tcaaacgagg
     5941 gaaatacaaa tgaatccaaa taatggtggg aatcatcaac aacagaatcg tcaccaagac
     6001 cacacctgct aataaaagtg agcgacccac cgcagcaata aattccctgt ccgtcagcag
     6061 ctgttggaac cattgcaagg taaaatcact aggtaaaagc gattttgacc atttagtgga
     6121 gctggcatac aaaatcgttc caataatcgg cagcaagtaa tacaggacaa agcagaacaa
     6181 aatcggtttt attaactttt tcacgtctgc accaccttgc ctttcttttg aataatcaac
     6241 attgccgtta ccagcaggac acctaaagcg actgccaacg cactgcctaa attaggcttg
     6301 gcgaacacat ctcccgacac taaagccgca attcgaatcg ttaaaagatt tacattactg
     6361 ctggtcaggg catacgctgt ttcataggtc cccatgccat tagcaaataa gatgacaaag
     6421 gtgctggcaa tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc atacgctgat
     6481 gaagcgccca gcaattgtgc ggactccagc cattcctttt tcacttcctt catgctggga
     6541 tacaaaaaca agacccctaa cggaatttgg aaataggtat acgtgatggt caatccccac
     6601 caagaataca aattgaagtc cgctagaaat ggcaaaatca ttttcagcac acctacattc
     6661 cccaacaaaa taataaaaga gaatgccagc ggaatcccag caaaattagc tgccaaattg
     6721 gaaacaaagg tgattttctc ctgcaattta gtcgatagat tcattaagca taaacataaa
     6781 ataaacgtcc cgattaaccc aatcacactg gagctgacag caatcagtac gctgttaaca
     6841 aaggcctgtg tgtaatattt gttgctgaaa atatcactgt aattggccaa tgtccactgg
     6901 ttagttaaat cactgtgcaa gctgcacaga accataaaaa gcagcggcag cagtaaaaat
     6961 gcacagaaga taaaggaggc aggaaccaga accaagcgtt ttttcatctc tttcatcccc
     7021 tcatcaattc ttgaatgtcc tgcattttct ctgacggtgc aattcctaat aaccagcaaa
     7081 acaatggagc cgccagcagc tgtggtacag tttgttcctt cagcttttgg cgtttatttg
     7141 aaatcaaata aaaaggaact tcacgatgtg cagccaagct gccgccatgc agtccgtgcg
     7201 catccatccc gtgatccgca gtcaccacga cttgataacc tttttgcagc cattcaggaa
     7261 ggtaaatagc caataatctg tccgcctgat taaccgctgc tgcatattca tgagaatcgg
     7321 cggtaaattt gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa aagtctggct
     7381 gatactgatt cactaaataa tgggcatccg caaataaatg cgaatcaggg taagtgtcct
     7441 cactataaaa gatgccatgc tgaatcagtc gctcctcctg atgctggatg cgatcaacca
     7501 aacgattata gggggcttga ttgtacaact cgctgtacca gtggtaagcg gcggctcctg
     7561 tttttccgcc agcttcctta actaattcaa acagcgacca ctctttggat cgctgagcat
     7621 ggtaattact atagatgcca ttttcatagg ctggaacgcc ggtcatcagt acttcgtata
     7681 acggacgtga attgctgggc agctcacttt ttaccttcaa taagcttgct tgctggtgct
     7741 caactaaatg attcatgaac ccaagctggg cgacggctgt gtcgtagcga cagccgtcta
     7801 gtataactac aatcagtttg tcaagtctac tttgcatagg tcagtacctc cgcctgccaa
     7861 agctctggaa gctctacaga ggtcttgttc caagcttcat tgtcttcaac tggcaccgcc
     7921 gcttcataag catcctccgg cagtaatttt tcagctactt ctttaggcag ctctaccttg
     7981 cgaataggtc gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga taaaatatat
     8041 tcccgtgcta gctttgccgc attgggatga ggggcatttt tattgattaa ggttgtatac
     8101 ccgctaatga tggagccgtc tgttggcaca gttacctcaa aacgctcgga gtcaatttgg
     8161 tcacgataat ttagtccatt gaagtcccaa acaactgcca catcgatttc acctttttca
     8221 agatttgcta cgctggcatc aacggtggac aaacggcctt tttctgccat ttttttgtag
     8281 taattcagac ctggctgaat atccttttcg tcacctccgt tggcaatcgc cgcagccaat
     8341 acagcaaatt gtgcttggtt ggcaatcgtc acatccccca cgctaacttg atagtcaccc
     8401 tcttgcagct ccttccagct agtcggtgct tgcttcacat tctctttatc ggtgataaag
     8461 gaaatggttc ccgtatagct taacaaccaa tccccatcat catcctttgc ccaatccgga
     8521 atttcctccc aatagttggt cttataaggt aaggttaact ctttgctttc agccaacgga
     8581 ccgaagctga taccgacatc accaatatcg gcggttccat tttttccttc tgattcaaac
     8641 ttagccaatt cttctgcact ggacatatcc gtatccacgt gacttaagcc gtattctttt
     8701 tcaaggtcgt tccaagtgcc tacccaattt gcccaagtat ccggcattcc caagctggca
     8761 atctctcctt cttccttggc tttttctata atcgtatcta aagagtcgtc tgccgcagcc
     8821 gattgcttcg ttgcttcacc tgagccgcag cctgtcagca ggatacttac tgttgctgca
     8881 caaaataaac cgatagtcat gttacgaatt ttttcattca ttttcattca ttcttctccc
     8941 ctttcgataa ttccattcta acaaagcttt gtaatgagag aatcagtttc ttgaaaaggt
     9001 ttcatgaaca atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc ccgttaacct
     9061 tctaaagctt ttgcggtatg atagtagaaa aaaggaggct ccctatgcag tattatgatc
     9121 agcatctcca cacgtatttt tcctttgatt cagaggaaaa atttgaaaac tatttagcct
     9181 atcagccgga gtttttcgtt tctacggatc actttgattt aaacaatcca tctaccggaa
     9241 aagatgacat tccggattat ccagcctata taaacaaact ggactcttta aaagaaacct
     9301 attccaccaa atttctgcga ggtatcgaga ttggcgttgt tccaggacaa gaagagcgga
     9361 tcgtggatta cctcgctcag catccttatg atttaaagct ggtaagtatt catcaaaatg
     9421 gccgctttga ttatatggac gatgtggttt taaccaaaga tcccattcaa actaccaagg
     9481 aatattttga tcagatgttg caggtcttga cggattttcc agaaggagat attttgactc
     9541 actttgatta tggattacgc cgcttcacct tcacagcgga agaattcgcg gagcattttg
     9601 aggctcagct gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg atggagttaa
     9661 atgccaagag cttcttgaaa tacggcaacg aagaccttta ccgttatgcc gttcctttgt
     9721 atcaaagtgt cggcggaaag ctctttacct taggctcgga tgctcatgtt gccgaagatt
     9781 atcagctggg ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta gaaaagttgc
     9841 tagtcattca aggaaaagaa cgccgcctga cgccgctgcc gattttgtga ttcctatata
     9901 atagaagaaa gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag aggcacccat
     9961 ccattattta attgcgcgac attgggatgc gttgaaaatt gaggcggttt gccgatcctt
    10021 gttagcagcc gttccaaaac aacaactgga aaattttttg gtggctgaca gcttgcagcg
    10081 ggaaaaagtt caggcttatt ttgcagcctt taaagatcag cctttggaat atttgcatgc
    10141 gcagtttcat cttttttatc aagtagccgc cccagatgac tacaacgatt tacgaggaca
    10201 gctgcaattg acctttcaag cggatgaaac cgcctatacc gttttattgg gaatggctcg
    10261 ccttggtgat caggcgaagg tcgagtggcg gattttcgat atttgatggc aggatcaagg
    10321 cgtttcagtt gcaaaccttc ctctctttca attagaaaaa ccgcagcagg aagaagtttg
    10381 tgtgctggtc accgatgctg gtgaaattcg tctccgctta tttcctgacc aagctcccct
    10441 ggctgtagcc aactggcgct ccctagctga gcaaggcgtt tatgacaaca cacctttcgg
    10501 ctcgtgtcat taaggaattt gttattcaag gtggcgcttt ggatggttct ggtgatgaag
    10561 ctgtttccag ctatgagggc tttttcgctg atgaagtgca tcgcggactt tatcatttta
    10621 atggcgcctt ggccttaggg gaccacggcc cccacaccaa cggcaaccag ttttttattg
    10681 tgcaaaacac aaaggcgcag gctgacctgc taatgtaaag caagcatact tggagcttgg
    10741 aggactaccg gagctggatg gtcgttatac tgtttttgga caggtatacc aagggttaga
    10801 aatcgtagca gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga ttgagccagt
    10861 gttcattcga caaattaact tcgaaaaaac atcgtaaaaa gacactcact gacttcagca
    10921 aaagctctat tcttgagctg gctgcagcta gtgagtgtct ttcatttatc cataagataa
    10981 agcttggatt caataaataa ataaatgttt tggttaaagc tgtgaccagc aaatgaggaa
    11041 ccgtgcgagc cagttgatct gccaatgcaa acatttcttt aggtgaaggc gctggcgtgt
    11101 ctgccaacgg atactccagt cccaatgctt ggtaaccgtg cataccatag cagtggtacg
    11161 gtagtaattc taacggtacg aaccgatcaa gatctgccac aaaacaagca atcgtcagca
    11221 gctcggtcag gttcatattg atacctggaa ccacaggaac tcgaattcgc acttcgcagt
    11281 taggatactc aattaaaaag cgacgaatat tcttcaaaat ttgttggtta tctaagccgg
    11341 tccattgttt gtggagcttg ctggtaaaca tttttacatc cacaaacaat gtatccacat
    11401 agggagctac cttttggatt tcattataag caccacaaaa gctggtttcc agtacggtat
    11461 tgattccaat gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc tgaaccagtg
    11521 cctcgcctcc gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca gcatccttct
    11581 caatctcatc cattacctct tcagcggtca tggtctggcc aaatcctttt tcaatctgct
    11641 tgctttgtga ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc tttaaataaa
    11701 caacggtccg taaaccagca ccatcatgta gagaaccctg ttctattctt aatactgttc
    11761 cttccatata gatttttgaa ttacacacta gtttaaacta aaacacacaa ggaaaaactt
    11821 accatttaat cataaaatta gctcatataa atatactagg atgtaaaata gactggtcaa
    11881 aatctggtca aaaaagagga ctaatacagg tagtcctctt ttttgttatt ttaatattct
    11941 aaatcatttt ttattggttt attcccttat tcgatgtaga ctcaaatcac tatatcacta
    12001 gatatcaaac ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta aatggtcatt
    12061 gaataacatt caaaatcaat aaaacggagt cagacacgga gtcaaaaacg ctgagagtaa
    12121 aaaatatata cagccttgta ggtaaagtac cacagggctg tatatttttt atttcagatt
    12181 accttaaatg attccattta ctttttatct cattttttcc tactagttag tatcaaatac
    12241 tctcctattt tctcaataaa ctatttttgg atacatggta tctgaaacag ctaaaacctt
    12301 gtcattacag caaaaaatca cgtta
//
LOCUS       DAZULO010000080        12309 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000080 DAZULO010000000
VERSION     DAZULO010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.082"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            289..696
                     /locus_tag="ACG8OA_002175"
     CDS             289..696
                     /locus_tag="ACG8OA_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976930.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            710..1111
                     /locus_tag="ACG8OA_002176"
     CDS             710..1111
                     /locus_tag="ACG8OA_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976931.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1113..1484
                     /locus_tag="ACG8OA_002177"
     CDS             1113..1484
                     /locus_tag="ACG8OA_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976932.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1520..1819)
                     /locus_tag="ACG8OA_002178"
     CDS             complement(1520..1819)
                     /locus_tag="ACG8OA_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS4976933.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1825..2055)
                     /locus_tag="ACG8OA_002179"
     CDS             complement(1825..2055)
                     /locus_tag="ACG8OA_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976934.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            2672..3334
                     /locus_tag="ACG8OA_002180"
     CDS             2672..3334
                     /locus_tag="ACG8OA_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976935.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            3413..6638
                     /gene="fss3"
                     /locus_tag="ACG8OA_002181"
                     /pseudo
     CDS             3413..6638
                     /gene="fss3"
                     /locus_tag="ACG8OA_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            6752..7066
                     /locus_tag="ACG8OA_002182"
     CDS             6752..7066
                     /locus_tag="ACG8OA_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4976936.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            7079..7453
                     /locus_tag="ACG8OA_002183"
     CDS             7079..7453
                     /locus_tag="ACG8OA_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4976937.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            7463..7807
                     /locus_tag="ACG8OA_002184"
     CDS             7463..7807
                     /locus_tag="ACG8OA_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFS4976938.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            7878..9230
                     /locus_tag="ACG8OA_002185"
     CDS             7878..9230
                     /locus_tag="ACG8OA_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS4976939.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            9290..10432
                     /locus_tag="ACG8OA_002186"
     CDS             9290..10432
                     /locus_tag="ACG8OA_002186"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFS4976940.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            10457..10711
                     /locus_tag="ACG8OA_002187"
     CDS             10457..10711
                     /locus_tag="ACG8OA_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976941.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            10967..12151
                     /gene="mobT"
                     /locus_tag="ACG8OA_002188"
     CDS             10967..12151
                     /gene="mobT"
                     /locus_tag="ACG8OA_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS4976942.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            12148..12285
                     /locus_tag="ACG8OA_002189"
     CDS             12148..12285
                     /locus_tag="ACG8OA_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFS4976943.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         4168 a         2017 c         2646 g         3478 t
ORIGIN      
        1 tttgagggga agtacatata gttgttgata tagttgctaa tattgttagc taatttgtta
       61 gtaaaactgt atacgaattt agatagtaaa aaaacagttt gttccttata tagaggcact
      121 tttactactg attaaatgtc agatatgctt acaaatatac ttataaatat gcttacaaaa
      181 ttagctaata tttccgttga caaagctttg ttactattgt taaactaaat tgactagcaa
      241 aagtgaaaca taaatttatt aaataaatgt aaaaattagg agaaatcaat gtcttataga
      301 gtacaattaa taatttcaga agatgtagaa agtcagcaat ttggcactaa tgttattaat
      361 aaagtaatta atccagctct ttctattaat gcgcctttaa ttcctactgc tttatcattt
      421 tctgtaacag ctgtggtgtc tgagatagag gatacagaaa agataaaaat agtagaaatt
      481 gaagttttaa acaagaatga aaaacaaatt ttttctacgg gtgaagtatc ggtaagcttg
      541 cctcctcaag ttaacgatat aaactttaat ataaacgcca gaaatgtctt ggttgaagaa
      601 gcaggagagc attatgctgt tgttaaattt aatggaactg agattggaag gcaaatcttt
      661 gatatcaagg tcaacaaacc agtggaaaaa aattaaggag acgatacgga tgcttgatat
      721 agtaccatca aatacacatc ggagtggtag cttaataaat tttgctgcga tttttgcttg
      781 cgcatcggta attctagcat ctccagttgt tgtggatgca agttctactc cgaaaactaa
      841 aaatgataat caacttgtaa ttactacaaa aaatgagatt aacacaattt caagtaatga
      901 tggcaaaatt tttgatgtga taaatacggt tttaaaaaga aaagctgaaa tcaatataaa
      961 atatgatgaa gatttaaact tgtatttctt tgttatcaaa acaacatctg aattgttttc
     1021 ctcagattac gatgttttgg atactctcga taatgtatta tctgactata aatatatggg
     1081 gaaatctgta gtagcaactt tgggggaata aaatgtttga ctttaatgaa tatttatcta
     1141 tttccaaaca aattgaatca gatgataaat ataattctaa agaatcttat agaagaactg
     1201 ctgtttcaag agcatactac agtgctttta aaaaatctga tgagtatttg aaagaaaatt
     1261 atgatattat ttataatgga agtagcggaa aaggaagcca ccaaaccgtt tggaatttgt
     1321 tttcaacagt taaggaattg aataccttag gaatacataa tagtgggtat agaatgttag
     1381 agaaaagaaa atgtgctgac tatattccta atgaaaccat tacgaaaaca gatatggcgc
     1441 tgatgaatcg agaagcagaa aaaataataa acaaactcac ctaatgaatt tcaagcgagt
     1501 ttgtttgtta tttttttatt taatctttgt atctttctgg atcaacgaaa gtatacttta
     1561 tatagtcata acgccgatga tcgctacgtg cgtccggcac gtcagtcacg atatcaaaca
     1621 aaaagtatac acccttcttc attctagttt tcgcagcagg aattttgaaa tagttcttat
     1681 tagaatagta gagattgatt aataagctat cttcgattgc taaaaagaaa acttctgaat
     1741 tccatacttt ataaaaatct ttgataaatc tattcgtagg atcgaattta aaccataatt
     1801 gtgtctcatt aaaaagcata accattactc cgatctgttt ttaaaattag tttctacctc
     1861 taatatatat cgagttttta ttttgccttc agagaatact gtttcttttt ttgcagttac
     1921 aggttgttta ttttcggaaa aagctaatat agctaaaatt gaaacatcca tctggaattt
     1981 atcttttttg ctgctttgct cataaaagtc tgcatattca tcactgatat tttttctaat
     2041 aaagtcttcc atcataaaaa tcacctcaaa acgattatac gaacttacgt tctcgttgta
     2101 aagcgatatt tgaggggcaa aaagggggca aaaatacgtt tatctttgat atatctcaga
     2161 ttgaacatag tgtttattat atgtcggtat tgttgattta tagctgtttt gagggtatta
     2221 gatacataat agaatatagt ggttttgttt acgtgcatta cacggaaaag ctgctcgtat
     2281 caaagaaatc agaagataaa cacggaaaac ttccttggaa atctgaaatt tgtgaaaagt
     2341 ccttgtatat caagggcttt ttcttttttc tctatcacag ttttaagcca tttttggctg
     2401 ttttggacca tttttcaatc atattccctt aaaaattgtc ctagattgtc cgacattgac
     2461 tatcagtgtc gtcaaaatag ggcaaaattt agtagtcagc ttgacgacaa atataatacg
     2521 aaaaatacca aaacatttac cgaatttagc aacttttgaa cccaagtttc tcaaagggtc
     2581 agctattagc aaaaaagaca aaacatgtac ttttctacta aaaagttgat gtgataggct
     2641 tctatctaaa taactattag gagtttttat cgtgaagcaa aatgatggac gtatcatttg
     2701 gaaaaatcaa agtgagttaa aactgatttt aacaattaat gaattcatcg aaaaacacgg
     2761 aattacatct agccgtcaat accagaaaaa gctatcagaa aatccaaatt ctgcaccaag
     2821 tatgtggttc attaacaaaa aatatggatc atgggagaat ttattgatta gtataggacg
     2881 agaaaatact gggtatggaa aatgggctag aatgtcagaa caagaattat tggaaatagt
     2941 agaagccttt attaagtgcg agaagataac ctctcaacga atgtacgaac aaaaatcagt
     3001 agggaagaat attccttcac ttagtaccat taaaaagatg ttaggagata tacgtccact
     3061 ttttaaagaa aaaaatgatg gatctcgatt tactgatttc gaactgctgt tagaactaaa
     3121 aaatgaaata atccgattaa aactacagga tgatttatca atgacaaagt ttcgaaaact
     3181 agttcaatcc ccaaaactac catcagttga tacaattatg aaaagaacga acaaaaattg
     3241 ggaggaattg atggcagaga ttggatttga ttatcgaaga atcaaaattt ataagcagag
     3301 aaacaaccta tccaagacaa agaaaactaa atagtatcac tctaaatcag acgttcaaaa
     3361 ttaaaatttg gacgtctttt ttattaccca aaaacgaaag gagatttatt ttatgaaaaa
     3421 gccaagattt aagaattggc gtttgctcgc aacactgagt ctattatgcc aaacaattgg
     3481 tggatcgctt ggcccaacga ttgcctttgc tgatgaaatt actcatccac aaacggtaac
     3541 tgtggaattg gatttggctc accagtatgc tgtcgaaggt acatttagcg atggtcgtcc
     3601 catgtcagaa gttaccgttc cgcactatgc agtttacaat ggtgtgaagc aagatatttt
     3661 ctgtattgag ccaggggtgc cgatttacaa tgagttcact cctggttatg agaaaaatcc
     3721 attgcctgat atgtcggaga aagcgaaatt agtttccgtt ttatggaagg aagctggtac
     3781 ggatgtggat acacatattg tggcccagaa gatgatttgg caagaagtta atggctacgc
     3841 ttcactcaat taaacgttct gatggtagtg cagtaaatat cgcagccatt gagtcaaaaa
     3901 tcaatcaagc cattgcggat tatcaaaaga aaccaagctt ccataactcc acagtgaaaa
     3961 ccgtgttagg tcaatcgact actgtgacag atacgaacgg cttgaattta tcggagtttg
     4021 atgaagttgt cgagaatatc gcaaatattg attatcgtgt gaacggcaat caactagtga
     4081 ttactccaaa tgccaattca aaagagagtg gtgaattaac cctaaagaag tcagctaata
     4141 caggaacacc ggtagcttat aaaatggctg gtcaacaaac tttgatggcg ggggcaattg
     4201 ataagcccaa tacctacaca atcaaaattg atgtggaaac tgagggttct ttgaagatta
     4261 aaaaagtcga taaagaaaca ggaaatgttg taccgggaac cgttttccat ttagactttg
     4321 gaaaaacatt acctgcaaaa gacgtgacca ccgataaaga aggcattgcg acattggatg
     4381 agattcctca tggcgcgaaa gtaaccatta ctgaaaaatc agtgccagct ccttatacga
     4441 ttgacactac accaatcacc gcaaccatta aatctggtga gactatttct gtgacttcaa
     4501 aaaatactcg cgaaaaaggt caaatcattt tgaataaaat gggggtggaa acaggaactg
     4561 atctttggaa cgacaaatac tcattagccg gaaatacgtt tgccattcgc aaagacagtc
     4621 caaccggtga aattgtccaa gaaatgacca cggatgaaaa aggtcatgcg gaaacaccaa
     4681 aagagattgc caatgcgttg gaattgggaa cctattatgt gaccgaaacc aaagctagta
     4741 atggctttgt gaataccttt aaaccagtaa aagtcgaatt gaactatgtc aatcaaaccg
     4801 tggctcttgt taccagtaac gtaaaagggc aaaaccaaga aatcactggt gaaaccactt
     4861 tgacaaaaga agacaaagat accggtaatg aaactcaagg taaagctgag tttaaaggag
     4921 ctgaatatac tctctttact gcaaaagatg gtcaagcggt caaatggagt gaggctttta
     4981 aaccagagtt agtgaaggga acgaaagctt ctgatgaaac ggtgactttg actttagatg
     5041 aaaagaatca agttgctgtt aaacacttag cgattaatga gtattactgg caagaaacca
     5101 aagcacctga aggatatacc ttggatgaaa caaagtaccc tgtttccatc aaaaaggtgg
     5161 atgataacga aaaaaatgcc gtgattaccc gagatgttac ggcaaaagaa caagtcattc
     5221 gctttggctt tgatttcttt aaatttgctg gatcagctgc tgggactgcc gaaactggct
     5281 tcaatgatct gacctttaaa gtatcgccat tggaaggaac cagtgagatt acaggtgccg
     5341 aagatgaagc aattacagct tataacgagc aactaggttt tgatggctat ggcaagtttg
     5401 aaaatcttcc ttatggggat tatttacttg aagaagtaga ggctccagaa ggatttcaaa
     5461 aaattacgcc attagaaatc cgttctactt ttaaagaaaa taaagcggat tacactaaga
     5521 gtgaatacat ttttacgatt accgaaaaag gacaaaaaca accaattaaa acggtgaccg
     5581 ttccttacga gaaactgacg aataaagcat tttctgttag cttgaatcgt ttgatgctct
     5641 atgatttacc tgaagaagaa gatagcttaa cttctcttgg gacttggaaa gacggaggca
     5701 aagaattgac agaccttgat tctaccgagc tagttgataa attgagctat aacttgcatg
     5761 aaatcaaaga agactggtat gtggtagcgc aagccattga tgtggaagcg acaaaagctg
     5821 cccaagaaaa agatgaaaaa gccaaaccag tggtaattgc cgaaacaacc gcaacgttgg
     5881 cgaataaaga gaaaacagga acttggaaaa ttttgcataa attaaccgca gaacaagttt
     5941 tggataaaac catcgtctta ttcaactatg tgtatgaaaa caagaaaccc tatgaagcag
     6001 gtgatgatcc agtagcgaaa gatgctagct tgaacaatca agcccaaact gtcaattgta
     6061 cggtggaacg ccatgtttcc atccaaacaa aagcccacct agaagatggt tcgcaaacct
     6121 ttactcatgg ggatgtggtg gatatgttcg atgatgtgtc gattacccat gatgtactgg
     6181 atggctcaaa agaagctttt gaaacaattc tgtatgcatt acttccggat aatacgacca
     6241 aagaaatttg gaaatctggc aaaattgatt atgaagtgaa tgacaaagaa ttcactaaaa
     6301 ccgtacttgc ggaaaaagtg gacacaagta aatatccaga aggtacaagt tttaccttca
     6361 aagaaattaa ctatggcaaa gacggaaaca tcaatgggaa acacaatgag gatctaaaag
     6421 aaaaaactca aactttaaca cctaaaaaag ttccgacaac accaagtgca ccagaacaac
     6481 cggaaatacc aactgttcca agtgactctc aagaatctag tcctacagta aagacattcc
     6541 cacaaacagg ggagaaaaat tctaatgttc tgctgtttat tgggtttacc ttgatctttg
     6601 cgactgctgg gtattatttc tggaatcgcc gaaactaagg tgatgtgatg aaacaactaa
     6661 ataggaaaaa ccaacgtgcg cgaccgcctc ctaaaaaaag tggtcgcgct atttttgtat
     6721 ccaaaactaa aaatgaaaag aggaaaagaa gatggaattg aaatttgttg tgccagatat
     6781 ggccgaaacc tttggaaaga taaggtatgc cggcgaaggc gaagttttaa ctgaaggata
     6841 tgggcggaat actacagtaa ttggtcgtag ttatcatttg tattccagta agcaacgagc
     6901 cgatgatatt gaagtggtgg tagctgcgga agccggcgaa aaggattttg atcaagatca
     6961 accactaaaa gccgtgaatc cccatttggt tgccaaaggc tatgagattg agaatcgtgg
     7021 gtttaccgac tacgtgttgt atgtcgatga tttagtgaaa gcataggagg aaaagaagat
     7081 gagattaaca gaaggtattg ttgtagattc aggactaacg tttgggaaat tacgattttc
     7141 cgcattacgc cgagaagtac ggaagcaaaa tgaggatgga acggttagta acgaggtaaa
     7201 agaacggact tataatttga aatcctctgc gcaaggtcga atgatccaag ttagtatccc
     7261 agcaaatgta cccttacgcg agtttgccta tgacgcagag gtagagttgg tcaatccaat
     7321 catggatacg gtcgccaatt atgttttccg agaaggaact actgtcaatt ggttcattaa
     7381 ggccgatgat ttagtcttga aacgacaacc agatcaggga aattctacaa accaaaatga
     7441 aggaaagaaa taggtgaagc gtatggaaat taatgttgaa catattttag attgtctcga
     7501 tcaatatgga aaaggtgagc taacggagga acaactcact aaagcattaa cctatgatga
     7561 aaaaatgttt ctgattatgc atcaagggtt actggaagta ggtaacgatg ccgaagaaga
     7621 ttttgatgta ctgaattggt taggggaaga agagtccttc tttatggtta tcgaagtaaa
     7681 tgaaagtcta tgtcgagaag ccgaaaccgt attggaagaa attggtgtgg aaatgccgga
     7741 tgccattgaa gtttttttaa agcaactagt ggaaacgaaa caactcccta tagcagtaaa
     7801 tgactagcat aactgcttga aattggatca atgaaatcct atgaagcttt tttcgattgg
     7861 aggtggaaac cataaaaatg tacaaagggc atcgtatacg ggcaggagat cagcacttgg
     7921 tttatcactt tgttcttggc tggctactcg ctctgttcat cggttggatg agcgtctttt
     7981 attttcaaga gcttagacaa tttgatattt ctaagctatc actttctact atagaaatcg
     8041 ttcgttctat aaaagattta atctatttgc taggaagtct cgttctttca ggaagcacga
     8101 tgttgcttta tatacacttt tttcaggatc attggcgcag tttgtggcat cgacaaaagc
     8161 tagcccggat gattctagaa aatcattggt acgaagtaaa acaaactcag agtgaaggat
     8221 ttttcaaaga cctcaatagt agtcgaacca aagagacaat cagttacttc cccaaaatct
     8281 attaccgtat gaaggatggt ttactttcta ttcgcgttca aatttcactg ggaaaatatc
     8341 aagatcagct tttaaagctg gaaaagaagt tagaaagtgg tctatattgt gaactagtag
     8401 aaaaggaact caaagactcc tatgtggagt acactttact gtatgatata attgccaatc
     8461 gaattgggat agacgaagta gtggcagaaa gtggggcttt gcgattgatg aaaaatcaag
     8521 tatgggctta tgattcttta cctcatatgt taatcgctgg cggcacaggt ggtgggaaga
     8581 cttacttttt actcactatc atcgaggctt tattaaaatc tgatgccgag ctttttgttc
     8641 ttgatcccaa aaatgcggac ttagccgatt taggtacggt aatgcctcat gtctattccc
     8701 aaaaggagga aatttctgct tgtgtggaag atttctatga gcgcatgatg actcgtagca
     8761 aggcaatgaa agaaatgccc aactacaaaa caggagagaa ttatgcgttt cttggacttc
     8821 caccaaactt tttaatcttt gatgaatacg tggtttatat ggaaatgtta acgacgaagg
     8881 aaagtgcggt gattttgaat aagctcaaac aaatcgtgat gttaggtcgt caatctggtt
     8941 tctttctgat tctagcttgt caaagaccgg atgcgaaata tttaggagac gggattcgcg
     9001 atcaatttaa tttccgggtg gctttgggtc gtatgagtga actgggatat tccatgatgt
     9061 ttggcgaggt tgataaaaaa ttctttatga agcgtatcaa aggtcgaggc tatgtagata
     9121 cgggagggag tgtgattagt gagttttata cgccacttgt accaaaagga tatgatttct
     9181 taagggaaat tagcgacatt ggtggattaa acgttaatac ggaaggataa aataatagta
     9241 tgtagcctag gagtgtcaag aatcctgggc tttttttacg aggtgattta tgacattttt
     9301 agatttattc gctgggattg gtggcttttg cttaggtatg gaacaagcgg gtcaccagtg
     9361 tatcggcttt tgtgaaattg atgattttgc tcgccagagt tataaggcaa tccatgatac
     9421 aagtaaggag gtggaaatgc atgacatcac aagtgtatca gacgagttta ttcaatctct
     9481 cggaccagtg gatatccttt gcggtggatt tccgtgccaa gctttttcaa ttgcgggaaa
     9541 gcggcaagga ttttccgata ctcgaggtac tttattcttt gaaatcgctc ggttcgccgc
     9601 tattctccaa cctaagtttt tattccttga gaacgtccgg ggattactca atcacgaagg
     9661 aggggctacg ttcgagacga tcctccgaac gctggatgga ttggggtatg atgtggaatg
     9721 gcaagtgctt aactcaaagg cctacgttcc ccaaaaccgt gagcggatct tccttatcgg
     9781 acattctcga gacgcatgta ccgaacaagt atttcctatc attggatcgt ctccaacatc
     9841 tgatcaaaac atcagaaact tattaaatat caacccttcc aatcgaggaa tggggggaca
     9901 agtctatggg tcagatgacg tagcgcctac cttgaccggt gatgaaggaa tcaaaatcgc
     9961 attgccggta gatgatggaa tttctgtagc gggtatgtta ctgggaaatt ttgaacaagg
    10021 caatcgggtt tatgagataa ctggaacagc gcctactttg tcaacgaaac aaggcggaac
    10081 gaagatcatg cttcggaaaa acgaaacaga atatcaagag gtgaagccag ggaacagtgt
    10141 gaacctggct tttcctaatt ccacaagtcg tcgaggccgt ctgggaaaac aaagtgtgca
    10201 taccttatta acaggagacc aacaagcagt cgtcacggat cagtatcaaa ttcgaaagtt
    10261 aacacctagg gagtgctggc ggcttcaggg ctttccggac tgggcttttg atcgagcttc
    10321 tcaagtcaat tcagatagtc agttgtataa gcaggctgga aattctgtaa cagttccggt
    10381 catttttgat attgctagaa gattaaagga ggtaaaaaca gatgatttat gatgagttga
    10441 ttggggaatc tactgggtga ttggcaagat tcagtcagat ccggaattgg aagaagaact
    10501 gtaccggctc aattttgata ttcgcaaaaa cggggtgaaa gtccctgatg atccgtatgt
    10561 aattgatgag gaaacggatg ctcgaatgga gctgaatcaa gtcatcagtg aattggaacg
    10621 aattgcggac tttgctaaag aacctgatat ccgccaatac ttgtttgaga taaaagcaga
    10681 gttggaaatt gagggtatta ccgcggagta acggccaaat aaaccactga taggcactga
    10741 ccgcggggga tttgcgaagc agaaatcctc cgtggtgctg tgcctgttta tggacggcgt
    10801 tagccggcca tgatgtctca ccccccgtat ctaacagggg ggtacaaaaa cacaaaaaat
    10861 aagcaagcaa cgataaggaa tggctgtcct atcaaggata ataagcaatt tgtgtagatg
    10921 ttaactgatt tgaaaaagtt aacaccttaa atttggaggt gagaacttgg cacaaaggaa
    10981 tttagattat cggctattaa aagaccggcg taatgaatat ggcgtttcac aaaataaatt
    11041 agctacgact tgtggactta gtcgcccgta cttaaatcag atagaaaatg gtggcgtaac
    11101 cgcatcgaca aaaaccatga ggaaaatttt tgaccaactg gaaagtttca atcccgagtt
    11161 acctttaacg ttactgtttg actatgtaag gattcgcttt cccacaacgg atgcgaggaa
    11221 aatcattcaa gagattctcc atttgaaatt tgattatatg ctccacgaag attacgcctt
    11281 ttactcctat caagaacaat atgttatggg agatattgtg gtgatgttgt cacatgaaga
    11341 agataaaggc gtccttttag aattaaaggg tcgtggttgt cggcagtttg aaactttttt
    11401 actcgcccaa aagcgtagct ggtatgactt tttcgaagat tgtctaaaag ccggtggcgt
    11461 gatgaaacgc ttagacctgg caatcaatga tcgagttggc ctgttagata ttcctgattt
    11521 aacgaaaaaa tgtcagaagg aagaatgtat ctccttgttt cgtaccttca aaagctatcg
    11581 ttccggggaa ctgttgaaac ctgatgataa agatggtatg ggcaatactt tgtatattgg
    11641 cagtcttaag agtgaggtct atttctgttt gtacgaaaaa gactatgagc aatatatcaa
    11701 gttagggatt cctttagacc agaccgaaac gaaaaatcgt tttgaaatcc gactgaaaaa
    11761 tgatcgagct tatcatgcaa ttcaagattt gttaaaaggt cgcagtattg aaagtaccac
    11821 attttccatt attaaccggt acttgcgatt tgcggataag gtggaaggca aaaggcgaac
    11881 taactggccc ttgaatgaac aatgggggcg cttcattgga cggaatcaga aggaaattca
    11941 attaacttct gaaccgaagc cttataccat tgaacgaacg ttgaactggc ttggacgaca
    12001 agtcgcccct acgtggaaga tggccaagga gttggatcgg ttgaatcaga caacgtacat
    12061 tcaggatatg gtaaataata ctcgactatc agaccggcac aaaaagattt tggagcaaca
    12121 aagtatggca attgaaaatt tgattatatg aaaagaggaa tcgtgatgga aattgtgaaa
    12181 gatattttat tggtgtttgg tggcagtatg cttggtgtta ctgtgatgtg cttgatgcat
    12241 gcaagtgcag cggcggaccg agcaatggaa aagaaaggaa agtgatgaaa tgaatttcgg
    12301 tcaaaattt
//
LOCUS       DAZULO010000081        12225 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000081 DAZULO010000000
VERSION     DAZULO010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.083"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..106
                     /locus_tag="ACG8OA_002190"
     CDS             <1..106
                     /locus_tag="ACG8OA_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4976944.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            583..783
                     /locus_tag="ACG8OA_002191"
                     /pseudo
     CDS             583..783
                     /locus_tag="ACG8OA_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1495..2100)
                     /locus_tag="ACG8OA_002192"
     CDS             complement(1495..2100)
                     /locus_tag="ACG8OA_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4976945.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2282..2617
                     /locus_tag="ACG8OA_002193"
                     /pseudo
     CDS             2282..2617
                     /locus_tag="ACG8OA_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2782..2997
                     /locus_tag="ACG8OA_002194"
                     /pseudo
     CDS             2782..2997
                     /locus_tag="ACG8OA_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3135..4106
                     /locus_tag="ACG8OA_002195"
     CDS             3135..4106
                     /locus_tag="ACG8OA_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4976946.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4246..5559
                     /locus_tag="ACG8OA_002196"
     CDS             4246..5559
                     /locus_tag="ACG8OA_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFS4976947.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5602..6468
                     /locus_tag="ACG8OA_002197"
     CDS             5602..6468
                     /locus_tag="ACG8OA_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4976948.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6465..7313
                     /locus_tag="ACG8OA_002198"
     CDS             6465..7313
                     /locus_tag="ACG8OA_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4976949.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7327..8799
                     /locus_tag="ACG8OA_002199"
     CDS             7327..8799
                     /locus_tag="ACG8OA_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFS4976950.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8816..9688
                     /gene="scrK"
                     /locus_tag="ACG8OA_002200"
     CDS             8816..9688
                     /gene="scrK"
                     /locus_tag="ACG8OA_002200"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS4976951.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(9924..11306)
                     /locus_tag="ACG8OA_002201"
                     /pseudo
     CDS             complement(9924..11306)
                     /locus_tag="ACG8OA_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11768..12091
                     /locus_tag="ACG8OA_002202"
     CDS             11768..12091
                     /locus_tag="ACG8OA_002202"
                     /inference="COORDINATES: protein motif:HMM:NF015824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFS4976952.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTHFGSVAKFSK
                     KSILV"
     gene            12123..>12225
                     /locus_tag="ACG8OA_002203"
     CDS             12123..>12225
                     /locus_tag="ACG8OA_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFS4976953.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQEL"
BASE COUNT         3806 a         2080 c         2454 g         3885 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagacattt
      121 aaaaagaaaa gtgacagata ggaataaaaa cttattaaat gtagttatga tctgttatat
      181 ttcatttcta atactaggtc ttccacaagc agtgtttcct gaaatgtttt ctggagactg
      241 gggtattgtc agctattcta tattaacatt gattgctatg gttttaggta cttcatttta
      301 taatgctttt gtaagtaagc atgttgagga atatgaagta ttaatgtact taaaagagaa
      361 aaaaacaagt aattaatatg agaacaccac gcatagtttt tattttgaga tattctagaa
      421 tgaaacacat taagaacgaa agcaaagcaa aagacgccag aacttctact accttctaag
      481 ttttttcata tttgtttgtt atgctataca tagatctgca taattagact tgcgctgccc
      541 gttattgaag tttcaggcag cgctcttttt gggggatcga taatggaaga agaaaaaata
      601 tttgagaaac gctggcagct agcatcaagt gaacaaagag caagatacaa taatttgatc
      661 acctcctacc ccactgttga ctagacatat aaggaaaaga aatacttact ttggctgtgc
      721 cagttagata tagatacctt tgaaacattt gaagtgatcc tagataaaat taaacagaat
      781 tgatcagaaa caagcgaagc cttctctcgg ttcgcttgtt ttgctattta ttagttggct
      841 cagggcaact ctaagctcgt ttgaaagaaa accgatggat aatccccttg tttgtaccac
      901 tttagaatac atggatgtac gtattgccat tgactcctat tagttactga aaaaaaattt
      961 aaacaagttg agtattgcag ataaatagct ccagtgaggt gaataactgc tccactcaag
     1021 gcaatagata gctccatcca gggcaacaaa ctgctccact ttcatattta atagatgaat
     1081 aaccttctgt ataatagaaa ggcatacctt tcggtatgac aacttataca gaatgttatt
     1141 ttattggttc actatcgaaa aattttggag ctttattttt ccggtatttc tcaacgaacg
     1201 attagtttaa ctgtgggaag ctccagacat acgattcgag aagttacttc tcgagcagac
     1261 aggaaggggg tgactgaact aaccgctgat atgagcgata cttggctgta ggaatatctt
     1321 tttccggaaa aacaaacaac agcacgaggt taccttcctg tagactggga atatgttcat
     1381 aaagaactaa tgaaaaagaa tatgacattg aaactccttc atagagaata tggggtacat
     1441 ggaacataga aacgaaaatg cacggtagaa aagcgaatga atatagtata aattctacaa
     1501 atcattcgct ttctttattt tatatatggt tggtcgagag agaccatttt ctttggcaat
     1561 ttctgcaact ggaactcctt gatttaacat gtccaccaca gttttaaaga ctaatcgttt
     1621 ttgcggatct gttgagttct cagaatattc aattggtcgc cccttatatc gaccagcttt
     1681 tttggccaat aatactcctt gccgctgacg ttcacggatt ttctcacgtt cgttttgtgc
     1741 aatataactt agcaatgata aaaacatatc gaacatagca gtatctagga gttcatttcc
     1801 agtattaaaa tctaaaaact gtgcatctag tatttttaat gctacttttt tctgtttcat
     1861 aaaggtaact gtatttttta tgtcttcata atctctgcct aggcgatcca gtgatgcaac
     1921 aatgacacaa tcaccttctc ggatataatt tagtaatttt tgaaactcag gtcgctggat
     1981 atctttacca ctggcttttt cttggaataa tttatcaatg ttaaggtctt ccatcattgc
     2041 caattgtcga tctaaatttt gatcttgtga agacactcta atatatccaa tttttgccat
     2101 aaaaactcct tttaaaaaat gatataattt acatgtaaaa tttacttgta gttttactgt
     2161 tatcatagca tttttaaaaa taaattttaa gtctacttat aatttacttt ggaggttttg
     2221 aatgaatgaa attaagcgca ataaaaattc ttttggtgtt tatttaggag agcctagctc
     2281 ggaacagtta aataaaggtg aagctcgtca tgctctatgt cgaaatatat tttatggtag
     2341 aaaaggcaaa ctttatcaaa cttattacga cggtatggag gaacagctca acgcattagg
     2401 attagtaact aatgctatca tatattggaa ttcactatat ttagaaaaag tctttgaaca
     2461 aattaaaacg gaagggttcg actgttccga agaagtgtta cgcaagcaat ttccattaat
     2521 tagtgatcac attaattttg ttggcaaata tagtttccgc tatgaaaaag aattatcgaa
     2581 tggaatacta cgtccgttaa atatagagaa ttattgagaa tgttcgtctt ttggttccta
     2641 ccgtgcattt tcgcacggat gttccagata ctatttttga atgtataaaa ttgaaaacac
     2701 aagaatacta ataaatcaac gattcttgtg tttttaattt ttcactaccg tgcattttcg
     2761 tttctatgtt ccatgtaccc caatcttgaa aaattaatta aagacttaga taaagccatt
     2821 gacgacttag tcgtggtgct accggagtat caatgtttaa ccagtatccc aggtattggt
     2881 cctgtttagg ctgctggaat actcgctgaa attggtccaa ttgaacgttt ttctgatgaa
     2941 ataaaattga caaagtatgc cggcctatat tggcgacaga gtcaatccgg taaatctgaa
     3001 tacgaaaatt tttttacgaa aagcttcacg tctaatatct attttaaaat aaaaatattc
     3061 ctgctaattt ggaatcgttt ccttcttttg gtataataat acctgaaatt cttttgagag
     3121 gagttgttcg tgaagtgaaa actactatga aggatgttgc tgcgttagca ggagttggtg
     3181 tgggtacggt atctagagta ataaatggag taaaggtgaa agaggttacc aaagaaaaag
     3241 taatggctgc aattagtgag cttaattatg agcctgatga atatgccaga ggtatgaaaa
     3301 cgaatagtag caatacaata gctcttattt tacctacgat ttggcatccc ttcttttccg
     3361 agtttgccta ctatgtagag gaagctctca gccaaaaaaa ttataagctt tttttgtgta
     3421 atgcgaacgg cagtgcggaa aaagaaaaag aatatatcca aatggtcaag cagagtaaag
     3481 tagacggaat tataggtatt acctattcag acattgataa atatgtatca tcaaatttac
     3541 catttgtcag tatagaccgc catttttcgg aacaggtttc ttatgtaacg gcagataatt
     3601 atttaggtgg gcaaattgcc gctcaagaac tacttactcg taactgtaaa aacttgtgct
     3661 atgttggggg tgtttcacct taccctaacg aaacaaacaa tcgtaaagct ggtttttaca
     3721 atatttgtga ggaaaataaa atagctgtga aggtattgga tatgcctgaa ccaattagca
     3781 atttagagga acaattgctc gctttttttt cggataatcc tgaaattgat agcattttta
     3841 ccatcaatga tttcatggct ttggatgtga taaaagccca aaaaaaacta gggaaggaag
     3901 cacctaagga ttttcaaatt attggttttg atggagtcaa gctatcgttg caaaaagact
     3961 atttactatc aacgattgta cagccgatag aggaaatggc caaggtttca gtagaaacac
     4021 tcatggcgat gattttaaat gaagaaccgc ctaagcgtgt ggtagtaccg gttttctttc
     4081 acgctggaga gacgactaaa gagtaaaaaa ccggtttata acgatttttt tgagataggt
     4141 tgacagaaag tgatttcaat gatatcttaa aaccaattaa ttggaaacgt taccaattaa
     4201 ttgaaaaata aaacggaaac gtttcaaaaa ataggaggaa catcgatgaa aaagagaatg
     4261 ttagcaattg cagcaatttt agcattaaca gctggaggac ttgcaggatg tggcagtaag
     4321 gagaaaagtt caggtagcag tgatccagga gaaattaaga tttgggttca gttttctgat
     4381 gaaacgcctg aaggaaaagc ttgggcagag attgtgaaca attttaatga tgaatacgag
     4441 ggtgaatata aagctgttac tgagtatatt cctcgtagcg gaagcggcgg tggatatgag
     4501 gataaagtaa atgctgcaat tacaaccaat acccttcctg atgttattac gttagatgga
     4561 ccgaatactg cagcttatgc tcattcagga gtcattgctc cattagatga ttatttgaaa
     4621 gatgcggaca tggatgatgt attagacagc attattcagc aaggaaccta cgatggcaaa
     4681 atgtatgcgt ttggtttctc agagtccagt gtgggtgtct attacaataa gtcgctgttc
     4741 aaagatgctg gaattacaga agatcaattg ccgacgttag aagatccttg gacttggacg
     4801 gaatttcaag caatttgcca aaagctgact gataaataca aaacgcctgc aattgatatg
     4861 caaatggcgt cgggagacga aatgctgacc tacgcatata caccatttat atggtcgaat
     4921 ggcggcgatg tcgttaatgc tgatggaacc aaagccgaag actattttaa cggtaaaaaa
     4981 tccgttgaag cgttggaatt tattcaagaa ttggttgaaa aaggttacac cacaatcagt
     5041 cctattgaaa aaggatttga aactggcaaa tatgccatga tgctgagcgg ttcttggaca
     5101 atggcagatt tagaggaaaa ctatcaagag attgattatg gtattttgcc ttaccccgtt
     5161 tcagatgcaa ctggggagtt agtttcacca accggaagct ggcagttagc aatgactgag
     5221 aaaacagaga aaaaggatgc ttctgcggcc tttattaagt ttgcaaccag tacagaatct
     5281 agtcgtatta tgagtttagg gaacagtgtt ctacctatca gaaattcaac agttgattta
     5341 attaaagatg aagtgtcgga aggaatgcag gtattaatta agcaaaacca actttctgga
     5401 catgcccgcc cagtggttgt agcttatccg caagtatcta gagcattcca acaagcagtt
     5461 caagatatta gctacttcga ggaaaaccca gatgtacaaa aagtagcaga tagtcgcgct
     5521 gctgaaatgc aacaggcaat tgatacttca ctaaaataga agtttctaaa gggggccaat
     5581 caagtgttaa aaaaaccgac aatgaaagac aatttgatag gttacggctt tctagcgcca
     5641 gctttgctgc tgctgacgat ttttctgatc atacccgtaa tcatggtaat ttattatgcc
     5701 tttactgatt attatttgtt aacgccagat gctagacaat ttatcggctt agaaaatttt
     5761 aagcgactgt tcgctgatcc aatttttgta aaaagtattt ggaacacggc taaatttgtt
     5821 gtttggatta ttccggtcca aataggggca gctttaggaa tggcacttat tatcaacaaa
     5881 aatcgtaaag gaaatatctt ttttaaagta gccttttttg cgccagtcgt gatgtcctta
     5941 gtcgttattt cgattttatg gttgtacttg ttaaatccta atgaaggtct ggttaatacg
     6001 ctatttgcaa agattggaat tgcttcgcag ccttttttga ctagtcccaa acaagcaatg
     6061 tatacgcttg ttttcgtatc ggcatggcaa ggtgcaggat atcagatgct gctgttttta
     6121 gggggaatgc aaaatattcc gcaagacgtt tatgaggcag ccgaaattga tggtttttct
     6181 aagtttcagc aatttcgcta tatcaccatg ccattattaa agcccacggc gatttttatt
     6241 ttacttacca cattgatttc ggcctttaaa ctaatcattc agccaatggt aatgactcaa
     6301 ggtggaccaa tgaattctac tatgacaatg gtttattaca tttaccaaac tggatttaca
     6361 gaccgtatgg taggctactc cagctccatt gcgttaatct ttaccactat gattggttta
     6421 attacaattg ctcaaagaaa attggtaaag gaggatgatc cagcatgacc aagaaatttg
     6481 gaccgctaat gatcgtagaa tattgtttgc tgttcctttt agcggcatta ttcattttcc
     6541 cgatgctgtg gatggtagtt tcctctatga agcctgaagc agatgtctat caaaatttag
     6601 cttccttcaa agctttttta cctagtttga atccagctaa ttggtttaaa acttatcaag
     6661 aggttatttc ccggttcagt gtgggaactt atttattaaa cagtattttt tatggtttaa
     6721 cctttgcctg tgggtcaatc attgtaaact ctttggcggg ttttgccttt gcgaaggtta
     6781 atttttcagg caagaaaatt atctttggag ctttgttagc tctgctgatt gtcccgatgg
     6841 agacggtttt aattccgcaa tttaccatcg ttaattcact aggcttagtg aatactcgct
     6901 tggcagtgat tttaccagcg atggccagcg cgtttaatat ctatctgttt agaaatttct
     6961 ttatcgcgat tccagaagaa atcattgaat cagcgaaatt agatggtgca aatattgtta
     7021 aaattttctt tcacatcatg ctgccaatgt ctaaaccagc catagccaca gtaggtgtct
     7081 tatcctttat ctccagctgg aatgattata tttggccgtt aatggtctta actgacagca
     7141 gcaaattttc aatgcaagtg gcaattacta cgatcaatac tacacagcca gtttatatca
     7201 atcaagtgat ggctgtttta accattgcaa cgattccact gattattgtt tatatcgttg
     7261 cacaaaaata tattctgcag ggacttggcg gttctggaac gggtataaaa taggagtgaa
     7321 tagtgaatgt tgcaaaaact tttaaaggcc aatgagttta ttgagaaaaa tgctcaaaca
     7381 gtcaatcaag aataccggaa taaatttcat ttgatggctc cgattggctg gatgaatgat
     7441 ccaaatggtt ttgtttatta tcaagaggag tatcacttgt tttatcaata ttatccttac
     7501 gacagcatct ggggaccaat gcattggggc catgctaaaa gcaaggatct aattcactgg
     7561 gagcatttgc cagtagcact ggcaccaggt gaaagctatg acaaggaagg ctgtttttcc
     7621 ggcagtgcca tagaaaagga tggcaagctg tatttgattt atacaggaca caatgttgtg
     7681 gaagggcaag ttcgacaagt tcagtgtttg gcagtttcag aagatggcat tcactttgaa
     7741 aaatatgccg gaaatccaat catcgcagaa gatcacttag cttctgttgc aacgaccgaa
     7801 gatttccgcg atcccaaagt gtttcagcga aaagaagact attttactgt agtcgctact
     7861 aaaactgcag atgctcgtgg acaaattgtg atgttccatt ccaaagattt actaacttgg
     7921 gagttttatt ccgtattgct ggagggaact ccagaacaag gcattgtatg ggaatgccca
     7981 gacttgtttg aactagatgg caaagaagtc ttgattcttt ctcctattca aatgactcca
     8041 caaggcaatg cttaccaaaa tacgaactca actgttgctt tcatcggtac tgtcgattgg
     8101 caaacaggca aatttctagt agagaatttt catgaaatcg atggcggttt agatttttat
     8161 gcaccccaga cctgtgaaaa cgaagcaggc caaagaataa tgattgcttg gatgcaaatg
     8221 tggcagcgta aaatacctac gaatgatctg ggccatggtt ggtcaggttc catgaccttg
     8281 cctagagagc tgagggtggc tgataatcaa ttgttgcaac ggccagtttc atcaatttat
     8341 caagtaattg atccgcagga aaggattgaa tgggcgaaga ctgaagagaa tcgcttctta
     8401 ctgaaggact tagtatatga ccagcactat ttacgcttgg aaatcaattt aaaacaggca
     8461 acgaaactag aacttcacta tgcgaaggga cccgaccata cattactgct taactatgat
     8521 gttgaacggg agtgctttac tgcttccagg gaacacatgc cgtatgattt atcaggagct
     8581 gaaaaggaag tgttgaaaaa gcgcagtgtg acgattcctt tggaaaataa ttgtctaacc
     8641 atcgaaattt tccgtgatac ttcagctatt gagctttttc taaatgatcg tctgactatg
     8701 agtacaactt tttatgagac agaaaaagga caggattttc ttttgctggc agacagtgaa
     8761 gtaacctttt cattggaaag cggcttaatt aatctataga agggagagct cagtgatgat
     8821 ttatggttca atcgaagcag gcggcacaaa atttgtgtgt gccattagcg atgaagaaat
     8881 gacgatcaag gaaagagtga gctttccgac aacgacacct gaggaaacaa tacctttagt
     8941 cattgacttc ttcaaacaat accaagcaga tttagcaggg attgggattg gctcatttgg
     9001 gcctatcgat atccatcgcg attccgccac ttatgggtac attacctcga ccccgaaagt
     9061 ggcatggcaa aattttgatt tcatcggtac catgaaaaaa gaatttccga tcccgatttc
     9121 ttggacaaca gatgtcaatg cggctgccta tggtgagtat gtcttcggca gtggcaaagg
     9181 attgagcagt gtcgtttact atacgatcgg tacgggtgtt ggcggcggtg ccttacaaga
     9241 agggcgcttt atcgaagggt ttagtcatcc agaaatgggg catatgctcg tcgtccctca
     9301 tccaaaagat gactttgctg gcagctgtcc atttcatggc aactgtttag aagggatggc
     9361 cgctggaccc gccatcgaaa aacgcttagg gaaaaaagga caagaggttg cagaagatga
     9421 tccttattgg gaaatcgaag cctcctatat tgctcaatgt gcctacaata caaccttgat
     9481 gttttctcca gatgtgatca tttttggcgg tggtgtgatg aaacaacgcc atatgctgca
     9541 aaacgtccac gacgcatttg caaaattggt caatgggtat gtcgaaacac ctgagttaag
     9601 caaatatatc gtgaccccag cattagaaga caacgctgga acgctcggtt gtttggcatt
     9661 ggctcgagaa gcggtcttac attcataaac gaaaaaaggc tcttcgacat ttagtgttgg
     9721 tagagaaaga tacaactgaa aatttagtca taaccagtta ggcagagtgc ttaactggtt
     9781 tttgttttac ttggaagatc aagccttgaa tgagataagt tcttgctcga aagttatgga
     9841 aatttcgata gccgtaggct acttgtattg tgcaatagtt ttttacaaaa aaaagggggt
     9901 gcggatattt tttttggacc tttttaacaa atatatcttt gcttcttccg atattgaaga
     9961 gggcttagcc ttcctaaaga taacttaatt cgttgattat tataccaatg aatataatca
    10021 tccaatttgt ttataaactg attgattgtt gtattcttcc agtcacgttg ataaaacatc
    10081 tcgtttttta atcgtccaaa gaaaccttcg cacgctgaat tatctggtga ataactcttt
    10141 ttagacatag agtgaagcaa gttggctttg ttccaatagt ccaactaaca atagcaccat
    10201 cgtaacaatc gacaagtggt gataagtaga ttttccctgc gagaattcta aattcagtaa
    10261 tatctgttaa ccatttttca tttggcttct ttgctttaaa attccgattt aataaattgg
    10321 ggtgtgcagg agaaatttcc ccagcatacg aagaatactt ttttcgtcta gaaaatttaa
    10381 caaatagatt ttcctcagtc atcaatctac gtacaacttt ttcagaaagc tttttaccca
    10441 tattttttaa agcttggtga attctacggt aaccatacgc acagaagtta tgattgaaaa
    10501 tcgtgataat taatttgacg ttctttgtaa tacttatctg gtttctcaag tgcttgtttt
    10561 tgataaaaat agctgctttt aggaaactgg agctgttgaa gaagctcttt caaggtgaac
    10621 tgatttcgta aggcatcaat caatatcgtt ttttcttgat ttgatagaca aagaagattg
    10681 acgccttttt ctttttttag cagttcagcc gctccttcga gtaatgcttt ttcgagctgt
    10741 aattggtgta cttgtttttg taagtcttct acttccttta aaagtagctc tttttgtttt
    10801 tcctgatttt ttaaagtcat acacaatgga aaatcctttc caagaaccgt gtttttccat
    10861 tgatatagag taactctact aattcctcca gattcggcta aatttttcct attagtttgc
    10921 ggtttataga attttttcaa cacagcttct ttttgatctt gtgttaactt taccgcactt
    10981 tttcggagtt ttctagctcc tggtgcaagt tcttcacacc gctgccgcaa ggactctcta
    11041 ttaggataac caagcatgcg aatggttcgt gcataacatt gaccatattc aaaataatga
    11101 ttcaccgcta ttcgtttttg ttcttcagaa tactttgatt tttttcggtg ctcctgatgg
    11161 agctctccgg attccaaata ctctttatac catttccgta gtgcaccaat cgacggatac
    11221 cctaattctc gaatggttgc agcataagag caatcgtatt ttaaaaataa ctgaattgcc
    11281 cttacacgct ctttgtggga aaacacacgg acattcctcc taagtttatt ttttcgtcca
    11341 ggattatgtc cgcaccccct tgctttctga tgacttattc tggttctttg agtgtcaatg
    11401 catagatgtg atgtagctag ttagaaaaaa attttgaata ctcttttttt ctttcagact
    11461 cctttttaag taagattttg aagtgtgagt tacggggtca gaaacttaaa agtttttgat
    11521 cctttatttt caagtctgat ttggcagctt ttcggcagct catttattta tggtcagaaa
    11581 tagatgctga aattttaatc tattagcaaa tagtccagaa cgtatgaaga ggagtcggat
    11641 atcatttgtc attaacacca taaatattta taaaattcgt gttcacctac aattaaattt
    11701 aatatataat attcgttgta agggcttata attttttgtt aatgaacaag ataggaagaa
    11761 acatttaatg gacagttttc agagattgtc actagtaaat caatttgagt tattggctaa
    11821 atttgaggac caaaacagat cttattatga aagaaaaatc gaaatattga gagaaggtta
    11881 cgagtatcat tatgatgatg aaatttggag tgatctttct gagccatttc cgaaagaaga
    11941 ttctcgattt gtattagatg tattgaacat gtatcgagat atcaatttta gtaaatcaaa
    12001 gctaaactta gatgaaaacc aaattactaa ttcatactat actcatttcg gttctgttgc
    12061 aaagttttcc aaaaaatcta ttttagtgta aaattgagaa aaaagacaga gaggacagag
    12121 taatgaatca ttttaaaggc aaacaattca aaaaagacgt cattattgtc gctgttggtt
    12181 actacctgcg ttacaatcta agctatcgtg aagttcagga attgt
//
LOCUS       DAZULO010000082        12205 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000082 DAZULO010000000
VERSION     DAZULO010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.084"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..216)
                     /locus_tag="ACG8OA_002204"
     CDS             complement(37..216)
                     /locus_tag="ACG8OA_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976954.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(232..432)
                     /locus_tag="ACG8OA_002205"
     CDS             complement(232..432)
                     /locus_tag="ACG8OA_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976955.1"
                     /translation="MNKIIPLLFVLFVGEILMRHGAALHLNFVVFLGILVFCLIFWRF
                     VLTLVSLIFKLIAAIFLIGLFR"
     gene            complement(597..1304)
                     /locus_tag="ACG8OA_002206"
     CDS             complement(597..1304)
                     /locus_tag="ACG8OA_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976956.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="ACG8OA_002207"
     CDS             complement(1297..1734)
                     /locus_tag="ACG8OA_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976957.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="ACG8OA_002208"
     CDS             complement(1749..2000)
                     /locus_tag="ACG8OA_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976958.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="ACG8OA_002209"
     CDS             complement(2000..2206)
                     /locus_tag="ACG8OA_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFS4976959.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="ACG8OA_002210"
     CDS             complement(2643..2903)
                     /locus_tag="ACG8OA_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976960.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            3077..3181
                     /locus_tag="ACG8OA_002211"
     CDS             3077..3181
                     /locus_tag="ACG8OA_002211"
                     /inference="COORDINATES: protein motif:HMM:NF028245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFS4976961.1"
                     /translation="MPVETALGLMISFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="ACG8OA_002212"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="ACG8OA_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS4976962.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="ACG8OA_002213"
     CDS             complement(3810..4709)
                     /locus_tag="ACG8OA_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFS4976963.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="ACG8OA_002214"
     CDS             complement(4722..5315)
                     /locus_tag="ACG8OA_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFS4976964.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5391..5540)
                     /locus_tag="ACG8OA_002215"
     CDS             complement(5391..5540)
                     /locus_tag="ACG8OA_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976965.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5556..7760)
                     /locus_tag="ACG8OA_002216"
     CDS             complement(5556..7760)
                     /locus_tag="ACG8OA_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFS4976966.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="ACG8OA_002217"
     CDS             complement(7771..9231)
                     /locus_tag="ACG8OA_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFS4976967.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="ACG8OA_002218"
     CDS             complement(9292..11664)
                     /locus_tag="ACG8OA_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFS4976968.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            12074..>12205
                     /locus_tag="ACG8OA_002219"
     CDS             12074..>12205
                     /locus_tag="ACG8OA_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS4976969.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT         3040 a         2531 c         2053 g         4581 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtccgt ttcctcctcg taaaatatca tttgtctaat
    11701 agtttttatt caaaatgttt ctagattata aaatttattg aataacacat tcctaacgga
    11761 atttgtttcg atattgtgtg atttctcaga gtacaatgga tgtatggaaa ggatccaacg
    11821 ggaatttgct atttcctcct gtagcgtttg tctaccctgt ttagtctgtt acccgaacca
    11881 ctgttatcac taccaactca ttagctgttt cgataatgga ctagactttc aaatagtagt
    11941 ttggaaggca gcttctagct cttttatcac cgtattctat taggcgaggt tgtggctata
    12001 cagtgagttt ctgggaaatc ccacagtatc gaagcatttt ccaaaattta tatcgaaagg
    12061 aggtccttct tccatgaaat tatttgtcgg tattgacgtt agctctgaaa aactagatgt
    12121 ttgtttttta acagacgaca atcaattgtc tatcttatct gaaatctctg ttgccaatga
    12181 catcgaaggt gcttcattca tacga
//
LOCUS       DAZULO010000083        12191 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000083 DAZULO010000000
VERSION     DAZULO010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.085"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..630)
                     /gene="frr"
                     /locus_tag="ACG8OA_002220"
     CDS             complement(73..630)
                     /gene="frr"
                     /locus_tag="ACG8OA_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFS4976970.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(633..1355)
                     /gene="pyrH"
                     /locus_tag="ACG8OA_002221"
     CDS             complement(633..1355)
                     /gene="pyrH"
                     /locus_tag="ACG8OA_002221"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFS4976971.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1491..2372)
                     /gene="tsf"
                     /locus_tag="ACG8OA_002222"
     CDS             complement(1491..2372)
                     /gene="tsf"
                     /locus_tag="ACG8OA_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFS4976972.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2471..3253)
                     /gene="rpsB"
                     /locus_tag="ACG8OA_002223"
     CDS             complement(2471..3253)
                     /gene="rpsB"
                     /locus_tag="ACG8OA_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFS4976973.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3612..4091
                     /locus_tag="ACG8OA_002224"
     CDS             3612..4091
                     /locus_tag="ACG8OA_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS4976974.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4308..5399
                     /gene="phnW"
                     /locus_tag="ACG8OA_002225"
     CDS             4308..5399
                     /gene="phnW"
                     /locus_tag="ACG8OA_002225"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFS4976975.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5392..5520
                     /locus_tag="ACG8OA_002226"
     CDS             5392..5520
                     /locus_tag="ACG8OA_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFS4976976.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5524..6069
                     /locus_tag="ACG8OA_002227"
     CDS             5524..6069
                     /locus_tag="ACG8OA_002227"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFS4976977.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6523..8214)
                     /gene="argS"
                     /locus_tag="ACG8OA_002228"
     CDS             complement(6523..8214)
                     /gene="argS"
                     /locus_tag="ACG8OA_002228"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFS4976978.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8634..9581)
                     /gene="arcC"
                     /locus_tag="ACG8OA_002229"
     CDS             complement(8634..9581)
                     /gene="arcC"
                     /locus_tag="ACG8OA_002229"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFS4976979.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9696..10715)
                     /gene="argF"
                     /locus_tag="ACG8OA_002230"
     CDS             complement(9696..10715)
                     /gene="argF"
                     /locus_tag="ACG8OA_002230"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFS4976980.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10806..12035)
                     /gene="arcA"
                     /locus_tag="ACG8OA_002231"
     CDS             complement(10806..12035)
                     /gene="arcA"
                     /locus_tag="ACG8OA_002231"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFS4976981.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3339 a         2528 c         2203 g         4121 t
ORIGIN      
        1 tttttaaata aaagaaatat aaataaaaac agaggaagcg gaatgatccg cccctctgtt
       61 tttatgattt tattaaactt ccaataattc tttttcttta tcagcagcga tcttatctac
      121 ttctttgatg ctgttatctg tcaatgtttg tacgtctttt tctaatccgc gcaaatcatc
      181 ttcggtaata tcaccatttt tttgttgttt tttatattca tcgatcgcat cacgacggat
      241 gttacgaatc gcgatttttg cattttctgc ttctttttta acatctttag ctaattcttt
      301 tctgcgttct tctgtcagtt gcgggataac taagcgaatg acatttccat cattggttgg
      361 actgattccg atatcacttg ccataatagc tttttcaaca tcttgaagaa tgctcttgtc
      421 aaaaggtgtg atcattaaaa cacgtgcttc tggaatttgg attgatgcca tttggttcaa
      481 tggtgttggc gcaccgtagt agttcacagt aatacggtcc aaaaggctag cgttcgcacg
      541 gcctgcgcgg atttgcccaa gttcacgttg aagattttgt gctgcttttt tcattttttc
      601 ttttgcttct gtcataattg catctgccat ttttatttcc cccttacagt tgttccgata
      661 ttttcgccta agattgcacg gcgaatattt ccgtgttcat ttaaattgaa gacaagcagt
      721 ggaatatcat tatccatgct tagtgaactt gcagttgaat ccatgacttg taatcctttg
      781 gcaataactt ccagatgagt cagttcttca aattttactg catttgcatc tactttcgga
      841 tcagcagaat aaacaccgtc tacattgttt ttagccatca ggataacatc tgcgccgatt
      901 tctgccgcgc gtaatgctgc tgttgtgtct gtcgagaagt acgggttgcc ggtaccgcct
      961 gcaaagataa cgatccgtcc tttttctaaa tgacgttctg ctttacggcg aatatatggc
     1021 tctgcgattt gacgcatttc aatcgaagtt tgcacgcgtg tcggtacacc cacgttttcc
     1081 aacgtatcct gtaaagctaa ggcattcata actgtagcta acatacccat gtaatcagct
     1141 tgagcacgtt ccatgcccat ttgtgctccg atttgtccgc gccagatatt tccgccgccc
     1201 acaacgattg ccatttcaac gcccagctcg tgaacttctt tgatttcttg gatgatttct
     1261 ttgattactg gtggtttgat tccaaatcct gcgtccccag ctaaagcttc tccactcaat
     1321 tttaagacta cacgctgata tttcggtgtg accatgttca ttcctccaat attttctatt
     1381 gccattttac catacttaga tgatttcgac actctaagac tggtttttct tgttttgctt
     1441 aaaaaaaggg agcgcacaaa ccgtgcgttc ccgtctgtta ttcaacagaa ttattttttc
     1501 acttggctca taacttcttc tacaaagtta tcttcgcgtt tttcgatacc ttcgccaact
     1561 tcgaaacgta cgaatgtttt aacagttgcg ccttttgatg caacgtattt ttcaacagtc
     1621 atatcaggat ctttaacgaa tggttggtcc accaaagcga tttctgcttt gaatttcttc
     1681 aagcgtcctt ctaccatttt ttcaacgata ttcgctggtt tgccttcgtt caatgcttgt
     1741 tctgtaagaa cagttttttc atgttctaat tcagcttctg gaatttgtga ttcgtttaca
     1801 taacgaggat tgatagctgc tacgtgcata gctacgtctc ttgcaactgt ttcgtcagtt
     1861 gttccatcta gaacagtcaa tacggcaata cgtccgccca tatgcaagta tccaccaaat
     1921 gcagcattgt cgtctttttc tacaacttca aagcgacgga agctgatttt ttctccgata
     1981 acttgagttg cttcgatcaa gtcagattca attgtacctt tatctgtttt gattttcatt
     2041 gcagcttcca tgtctgctgg tttgttttca gcaacaagtt ctgcaatttc ttttactaaa
     2101 tcttggaaca tttcgttttt agaaacaaag tctgtttctg agtttacttc aacgattgct
     2161 gcagtgttac ctttcacagc aactgaagcc aatccttcag ctgcaatgcg gtcatttttc
     2221 ttcgcagcct ttgccattcc tttttcacgt aaaagatcaa ctgctttttc catgtcgcct
     2281 tctacttcaa ctaatgcttt tttcgcatcc atcattccta cgccagtcat ttcgcgtagt
     2341 tcttttacca ttttagctgt aacgtctgcc atgtttaact cctcctgtgt gtttttcttt
     2401 ttaaaaaaag ctgcttcaaa ggagagtgag gcttgtcgcc cagcctttga aacagctcca
     2461 tcattcaaaa ttattctgct gaagaattgt cgccttcaac aacatctacg atttcttcga
     2521 ttgatgttgc tgtgttttct tctgtgaaat cttcttcaac tacttgatct tcaccttggt
     2581 ttccttcgat gaaagcatca gccatttttg aagtaatcaa tttaacggca cgaattgcat
     2641 cgtcgtttga agggattact acgtcgattt catctggatc gcagtttgta tcaaccatag
     2701 ctacgattgg gatatttaat ttgtgtgctt cttgaacagc aatacgttct ttacgtgggt
     2761 caacgatgta cattacatct gggattcttg gcatatcagc gataccgcct aagaattttt
     2821 caagacgttc acgttctttg ttcaaaccag caacttcttt tttaggtaga acttcaaaag
     2881 ttccatcttc ttccattgca ttgatttgtt tcaaacggct gatacgtttt tggatagtat
     2941 cccagtttgt caatgttccg cctaaccaac gatggttaac gaagtattgt cctgcacgag
     3001 ttgcttcttc tttgattgct tcttgtgctt gtttttttgt acctacgaat aatgctacgc
     3061 cgccttcttc agcaacgctc ttcatgtaat cataagctgc atctactaat ttaactgttt
     3121 tttgcaagtc aatgatgtag attccgtttc tttctgtgaa gatatatttc ttcatttttg
     3181 gattccagcg acgagtttgg tgaccaaagt gtacgccggc ttctagtaat tgtttcatag
     3241 aaattactgc catgtttgtt tcctccgatt tggttttatt tttcctcttc ttatcttcta
     3301 ccttgccggc aaactttttc aagcacctac cgacaatcca ataagaatgt ggatttgctg
     3361 atgcataaag caccctcagc tattatacag acaaaagatc cgtatcgcaa gagcaaatat
     3421 acttttctgc tcaacattca atagtttacc tgaaagataa aaagaattca agaaaaacag
     3481 tagatttctc tttgtttctt tattatcctt tttccataaa ataattttag gtataaaaga
     3541 cgaatattat tgggcaatcc ttcttttttt tgttatgata catgtgaaat ctttcgtaaa
     3601 ggagaaagaa aatgcgcaaa gctcaaagac atttgctgat taaacaagtc attgaagaat
     3661 tcccgatccg tacgcaagaa gaactattaa acaaacttgg gcattatggc gttaccgcca
     3721 cgcaggcaac gatttctcga gatatccgtg atctaaaaat cgtgaaggca cctgatgaaa
     3781 atggcgtttc ccgcttcgta ttgtttcgcg aaaaaggaac tcctgagact aggactgagg
     3841 aagaggatcg gctggttcaa atgattgaag acattgtatt gaaagtcgaa cgagtccact
     3901 ttttgacgat cgtcaatacc ttgccagaca atgctcattt atttgcatct gttttagatg
     3961 agatcaaacc tccctctatt gtcagtacaa ttgctggatt cgatacgatc atggtcatct
     4021 cggaaaatga ggaggacgca aaacgagtag aagaatttct tcaccatcca actgaagtat
     4081 cactttttta atcagagcac gtattcctaa tatggaatgc gtgttttttt attttcaata
     4141 ttttccttat tgaataaacc tttacactct ttacaaaaat acatcatttt ttattcaaat
     4201 ttttcaacac cttgattgaa tcacaataat catcatttat attggatgtt aacaagagca
     4261 gcttcttgct ctttgtgtat ttcaaaaaag aaatgaaagg aaatcatatg tataaattat
     4321 taacttctgg accattgacg acttctgaaa cagtgaaaaa tgctatgctg atcgatcatt
     4381 gtacttggga tcaagaatac aaagaaatta cacaatggat aagaaaaaaa ttattggatc
     4441 ttgctcaagt atcggaagga atatacacaa cggtcttaat gcaaggtagc agctccttcg
     4501 gggtagaatc ggtattaagt tctgtgatca ggccagaaga tactgtactg atttgttcaa
     4561 acggcgcata tagacaacga atgatcaaca tgtgcgaatg tctgaaactg aactataccg
     4621 tttatgaggt atcggaacat caaatcctcc aagctcatgt gattacacag ctgattgaag
     4681 agaatccgtc tatctccgca ctagcaatgg tccatagcga gactacttcc ggtattttga
     4741 ataatttgga agagattgct gctgtcacac aagaaagaga tcttttgttt atagtggacg
     4801 cgatgtcaag ctttggtggg ataccgattt gtgtagccga actagggatt gattttttga
     4861 tttccagctc aaacaaatgt attcaaggtg ttccaggctt ttcttttgta cttgctaata
     4921 aagcggaact ggaaaaacga aaaaatgtta gccgaagttt gtcacttgat ctatatgacc
     4981 agtaccgcgt gatggaaaac gagaatggca aatggcgttt cacttcccct acccatacgg
     5041 ttttggcttt tcaacaagcg ctgaaggaac tagaggcaga aggaggaatc gaaactcgat
     5101 attcacgtta tctcgccaat aatcatgtgc tacgacaaaa aatgacagag atggggttcc
     5161 agcctttcat tcaagaatat caggggccat tcatcacctc ttttctttac cctaaggact
     5221 ggcgatttga ttttaatcat ttttatcagt atttaaagca aaatggattt gtgatctatc
     5281 ccgggaaaat cagcgatcat gaatgttttc gtatcggaaa tattggcgag atctatccgg
     5341 aagatatttt ggaactgaca gcaattatca aagactataa ggagaacgtt aatgattaaa
     5401 actgtaattt ttgattgggc aggaacaact gtggactttg gctgtatggc tcctgtccac
     5461 gcttttagaa atgcttttct agaaaaagga atccaactga ctgacaaaga aatccgctaa
     5521 ccgatgggaa aattaaaatg ggatcatatc caacagcttc tttccctccc ttctgtcaaa
     5581 aaacaatgga tagctattta tggacaacta cctcaggaaa aagaggtgga ggaattgtat
     5641 caacaatttg aacattatct gtttaaagat ttggtcaagc attctacctt gaaacccgac
     5701 acattggaaa cgatcgaacg gttgagaaac gctgggatca atattggttc cacgaatgga
     5761 tttaatgctc ctatgatgac aatcgtagca gaaaccgcaa aaaaagaaag gtattcacca
     5821 gacttcatgg caacttttga agacgtcgaa gggtatggtc gaccgtttcc ttatatgatc
     5881 cataaaaaca tgcagtactt caaaaacaag tcggttgatg aagtaatcaa agtcggagat
     5941 acggtctcag atatccagga agggaaaaat gccggagtgt tgacagtagg tgtaattgaa
     6001 ggtagttcgc taatggggat ttcttatgat gagtaccaaa aattagagat ggggaaaaag
     6061 atcgtttaaa acagtctgtt gctcttagat tccaacaagc cggcgctgat atttgtgtaa
     6121 acaatttaaa agagttaaca gaatacattt tgtacgctga catgaatcca gaatgacttt
     6181 tatacaaaat aagaggttgt gaaaaagctg tcctgtatca agtaataaaa acagccaagc
     6241 aaaaatagct cctcatattt ttcgtttggt taggcttatt accgcagatg caagcttttg
     6301 aacaccgttt attcgaaaaa aaaaagggag ctgtgacacg acttttgtca cagtcccctt
     6361 atttgcaaac aaacagtgtt taagagacaa cctctccact aaacggtgtt cattcagctg
     6421 actctcggta tttgctcaaa aaacgataaa atttgaaaag ctattttctc ttttgtttct
     6481 ctaatactcg gatcagaacg ctgatttcac attctctttt tcttacatct tatctggtgc
     6541 atgaagtcct agcaaacgaa ggtcttcttt caataataca gtcactgcat aaactagagc
     6601 taagcgtgct tctttttgtt catcatcagc taaaatcttc gtatgagcat agtatttgtt
     6661 gaatgcttgt gctaatttga ttgcatgttt cgcaatcact gacggttcgt atttttcccc
     6721 agcagacaag accgtttcag ggtatttttg aactaattta accacttccc agctggtatc
     6781 gtcattcaat gcatagtctg ctgtttctga agggacaaat cctgcttttt ccaacaggct
     6841 gactgcacga gcatgtgtat attgcacata aggtccagtt tctccttcaa atcgaacaac
     6901 ttcttccagg ttaaagtcaa atgtattcag tcgatcgttt ttcaaatcgt ggaaaatgac
     6961 tgccccaacc ccaacttgtt tagctacagc atctttgttt tccaaatcag ggtttttctc
     7021 gctgatttgt tctttagcag attggattgc ttcatttaat acttcttcta ataagacgat
     7081 ctttccttta cgagttgata attttttacc accttgcgtg atcaaaccaa atggaatatg
     7141 gtgcatgtcg tcagaccagt caaagcccat ttccttcaag acagctttca actgtttaaa
     7201 atgatagctt tgttcattcc ctactacata tagagattgt ttgaagtcgt aagtacgttt
     7261 acggtacaat gcagcagcta agtcacgtgt aatgtatagt gtcgcaccgt ctgacttctt
     7321 aatcaacgct ggattcaggt cataagcaga taggtcaacg atctctgcac ctttatcctc
     7381 gttcagcaaa tgtttttctt ctaaaagttt gacgatttca tccatcttgt cattgtagaa
     7441 agcttcaccg ttcaacgaat cgaaacgtac ttccagcaga tcatagattt tattgaattc
     7501 tttcatcgat tcatcacgga accattgcca taattgaata gcctctttat cgccttcttc
     7561 cagacgtttg aaccaagcac gagcttcttc atttaattct ggttctgttt cagctacttc
     7621 atggaattgg acgtataaac gaagcaattc attgattggt tctgctttaa cagcttcttc
     7681 tgtaccccat tttttgtaag cgacgatcag ttttccaaac tgtgttcccc agtcacctaa
     7741 atggttgatg cggattggct gataaccgat tttttccata atgaaaccga tcgaattacc
     7801 gataactgtc gaacgcaaat gtcccattga gattggtttg gcaatattag gagaagacat
     7861 gtcgattggt actgttcctt gattcccgat gttgctatcc ccataatgtt ctttttcttt
     7921 cactactgtt tgcaaaactt ttttgctgat aagttcttta ttcatgaaaa agttcaagta
     7981 aggtccgaca acttcgattt tttcaaagtt ggcagaatcg attttttctg ctaaatctgc
     8041 agcgatttgc tgtggcgctt ttcggtaaac ttttgctaat gaaaaggctg gaaatgcaac
     8101 atctccgtgt tcagctgatt ttggattttc tagtaattgc tcgacttgtt ccagtgttaa
     8161 atcatctttg acagcgtcat agacagcttt tgcgacaaga tctttattat tcatctttat
     8221 tcctcctaaa gtgttccctt gattgttaaa caaaaaaaat ctctagcgac ggcatactta
     8281 gtatgccatc actagagacg agatttcccg cggtaccact ccaattgtcc taaaaaagga
     8341 ccagctccat ctgatatcgg tagacgaccg tctgtttaaa acagaattct ccaagtgcgc
     8401 ttcgttgtat cttcttcatt cggcttccac cacttccgaa ttcgctagcc aagaatgata
     8461 caagtactct cttgttcaac aaatcttagt cttattaaac tttcttgaat tatatcagaa
     8521 aaaaaaacga aaggctagtc gtttcataaa aaaacaatcc agatggccta aaggagaaga
     8581 aataccacct ggatagctat aaatcatttc gtaaatacga aagacttaac aaattaatca
     8641 gctacgataa tcgttccttc ttcagaagct aaaagattct cgatattttc taaagaagtg
     8701 atgattgcct tagcgtttgg ttttgcttca acaaatgcaa tagctgcttc tactttcgga
     8761 agcatgcttc ctggagcgaa ttgcttttca tcaatgtatt gtttcatttc tgaaacagta
     8821 acagtttcta atttcttttg atctggtttt tggtaattga catacacatt gtctactcct
     8881 gtcaatacga tcagcaaatc cgcatcgatg atttcggcta atttttctga agcaaaatct
     8941 ttatcgatta cggcttctcg tccttctaat ccagtagctg tttctacaac aggaatccca
     9001 ccgccaccaa cagagactgt gataactcct tgatcgacca gcgtttcgat gacacgtgct
     9061 tctttaatcg agatgggttt aggggaagcg actacttttc tccatccacg tccagcatct
     9121 tctttgaatg tcacagtgga gtcagcattc atttgttctt tggcttcttc ttccgtgtag
     9181 aatggaccga ctggtttact tggatttttg aatgaaggat cattttcatc cacaataact
     9241 tgagtgacta aagaaaccac atctttatcg atcccttttt ccttcagtac ttcgcccatg
     9301 gcgttttgta accagtaacc gatagatcct tctgtcattg caacacatgt atctaaaggc
     9361 attgccggtg ttttctcaga atcagcagct tgctgctgaa tcaataggtt accgacttgc
     9421 ggaccatttc cgtgggagat gatcaattca tctccttgtt cgataaattt cactaaatac
     9481 tttgctgttt ccattaacgc ttcttgttgg gcttttgcac ttgcatccgt agacaagatg
     9541 gcattgcccc ctaaagcgac aactactttt cgtttggcca tttcattcgt cctttcttca
     9601 gttctaccga attttctaga taacaaaacg agccagcgtt ttgtgatttt ccctcaatgc
     9661 tgactcgttt tttcgtgaga tgaatgtggt tatgcttaaa cgcgagggat aaacaagtta
     9721 cctaatgttg ctgccatgat cgctttgatt gagtgcatac gattttctgc ttggtcaaat
     9781 tgacgagcat atttgctgcg gaagacttca tctgttactt ccatttcagt gatgccaaag
     9841 cgttctttca tttgttcacc gtaaacagtg tttgtatcat ggaaagctgg caagcagtgc
     9901 aagaagatca aacgatctgt gttgtgtgtt ttttctacca tttccatatt gatttggtaa
     9961 ggtttcaaca atttgatacg ttcttcgaat ttatcttctt cacccattga tacccataca
    10021 tcagaataaa ggacgtctgc tccgtctaca cctttatcaa catcgtcggt aatcatcaat
    10081 tgtgcaccgg atttttcagc aaagccttct gccattttga cgatttcttc ttcaggttgt
    10141 aattcttttg gtgcaacgat acgcatattc gctcctaaaa ttgcacctgt gactaataaa
    10201 gagttagcca tattgtttcg accatctccg cagtaagcaa cagtgatacc ttctactgtt
    10261 ccaaagtttt cttggatagt taaaaagtca gcgatcattt gtgttggatg ccattcatca
    10321 gtcaagccat tccaaactgg aacaccagag aattctgcta gttcctctac cattttttga
    10381 ctgaatccac ggaattcgat tccgtcaaac atacgtccta gtactttagc tgtatcttct
    10441 gtggattctt ttttacctaa ttggatatca tttgcaccta gatattcagg atgtgcacct
    10501 agatcgatcg ctgcagtagt aaatgcagaa cgagtacgtg tagatgtttt ttcaaataaa
    10561 agcgcaatat tttttccttc tagatagtga tgtgggatac cacgtttttt caagtctttc
    10621 aagtgttctg aaaaatcgat caaatattgt aattcttctt ttgtaaagtc tttttctgct
    10681 aataagcttc ttccttggaa tactgattct ttcatattaa acattctcct ttagattgtt
    10741 tttatttaag attaaagagg aaatcccgac ggcagctgac agtaagactg ccgtcaggaa
    10801 acaaattact tcaagtcttc acggacaagt ggttggctca tgcagcgtgg accaccacga
    10861 cctcgtgaca attcacttga attgatttct aacactttga tgccatagct acgaagcaat
    10921 tcgttcgata cataatttcg gttataagtg actacaacac ctggagcgat tgccaatgtg
    10981 tttgatccat cattccattg ttctcgaggt gcaacgatct catctccatt accagttggg
    11041 atcagtacaa gatcatctaa tccaagagca gctttcatcg tttcgtgtaa gttatctgaa
    11101 tgagtaatct tgatatcatc accatctggt tcgattgtaa agacatccat cttaccgtct
    11161 ttactttgaa ttgctggatg gatagtgaat ttatcgtgat ctaccattgt aaatacggta
    11221 tccaaatgca tcattgcacg gttgttcgga attttgattg ccaacacttt ttcaaatcct
    11281 gaattttttg cgaataggtg gcgtgctaat ttttctaaag ctttcgcatt agttcgttga
    11341 gaaatcccaa cagctactac tttatcgctt aagatcaatt cgtctccgcc ttcgatatga
    11401 tctggatttt cacgatccaa ccatacttcg attcctttat ttgcaaatct tggatgatgt
    11461 ttcaaaatat attctgtaaa catcgattca cgacgacgtg cttcaaaagt catcttattg
    11521 actgtcatcc catttcccat tgatgcggaa gggtcgcgtg taaagtacaa gtttggcatt
    11581 gggtccatgt aaaatggata gtcatcgtct gctgacagat catatagact gctagaacgt
    11641 acttcgatat cttttgtacg aaccccagcc atgattttat ctaccatttc ttgcgtttcc
    11701 attgatagaa ggaactcatg aagaccctct cttacagcat tcgatgcaat atgagattca
    11761 tccaacattt tattcaagaa ttgttctttg acattgcctg cgtcaatcgc ttctgccgct
    11821 aatttttcca agtaaagagt ctcgacgcct tcattttgca atgtcttcgc aaagttgtca
    11881 tgttcttctt gagcaatcgg aagatatgga atatcatcga acaacagacg atccataata
    11941 tctggtgtca aattttccac ttcttgtcca ggccgtttca gtaaaactgt ctttaatttc
    12001 cctatttcag agaaaacgtg aataggttta tccatgttgt gttgcctcct tctttttgat
    12061 tacatgtata ggatatcctc cttttgtaat cactttcata attttgtctc attcaaaatg
    12121 agacattcct cacattaata ttcgataatt aaataattaa acgtttttct atttttaagc
    12181 gtaatttatt c
//
LOCUS       DAZULO010000084        12007 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000084 DAZULO010000000
VERSION     DAZULO010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.086"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            81..323
                     /gene="yaaA"
                     /locus_tag="ACG8OA_002232"
     CDS             81..323
                     /gene="yaaA"
                     /locus_tag="ACG8OA_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFS4976982.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            310..1434
                     /gene="recF"
                     /locus_tag="ACG8OA_002233"
     CDS             310..1434
                     /gene="recF"
                     /locus_tag="ACG8OA_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFS4976983.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1431..3377
                     /gene="gyrB"
                     /locus_tag="ACG8OA_002234"
     CDS             1431..3377
                     /gene="gyrB"
                     /locus_tag="ACG8OA_002234"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFS4976984.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3397..5868
                     /gene="gyrA"
                     /locus_tag="ACG8OA_002235"
     CDS             3397..5868
                     /gene="gyrA"
                     /locus_tag="ACG8OA_002235"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFS4976985.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6104..6403
                     /gene="rpsF"
                     /locus_tag="ACG8OA_002236"
     CDS             6104..6403
                     /gene="rpsF"
                     /locus_tag="ACG8OA_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFS4976986.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6453..6965
                     /gene="ssb"
                     /locus_tag="ACG8OA_002237"
     CDS             6453..6965
                     /gene="ssb"
                     /locus_tag="ACG8OA_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS4976987.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6991..7227
                     /gene="rpsR"
                     /locus_tag="ACG8OA_002238"
     CDS             6991..7227
                     /gene="rpsR"
                     /locus_tag="ACG8OA_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFS4976988.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7370..9343
                     /locus_tag="ACG8OA_002239"
     CDS             7370..9343
                     /locus_tag="ACG8OA_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFS4976989.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9349..9801
                     /gene="rplI"
                     /locus_tag="ACG8OA_002240"
     CDS             9349..9801
                     /gene="rplI"
                     /locus_tag="ACG8OA_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFS4976990.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10075..11442
                     /gene="dnaB"
                     /locus_tag="ACG8OA_002241"
     CDS             10075..11442
                     /gene="dnaB"
                     /locus_tag="ACG8OA_002241"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFS4976991.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         4013 a         2178 c         2657 g         3159 t
ORIGIN      
        1 atatatggat aaatttgttt tattgcgaaa aaaaaggtat aatagagtat acttttaata
       61 atgaaaatga gggatgggca ttgaaacaac aagtcattct aaatacggaa ttcatgacgt
      121 tgggccaaat gttgaaagaa gtcacagtta tcggtagtgg cggacaagca aaatggtatt
      181 tggcagaaaa cactgtgtta gtcgatggcg aaccagaaaa tcgtcgtgga cgcaaacttt
      241 atcccggcat gatggttgaa gttccagaag taggaacttt ttttatggta aaaaaaggaa
      301 acgacgataa tgaggctgaa taagctgtat ttaaaaaatt atcgaaatta cgaagagctg
      361 aacatcgaat tttccaagaa cctcgtcatc tttttaggag aaaatgcgca aggaaaaacg
      421 aatcttttgg aaagtatcta cgtattagct atgacgcgaa gtcatcggac aagtagtgag
      481 caggaattga ttggctggac tgacgatcag gcgatgattc aaggagagat cacaaaaggt
      541 tcatccactc ttccattaga aatcttatta tctaaaaaag gccgaaaaac caagatcaac
      601 catatcgaac agaaaaagtt aagtacttac gtgggacaaa tgaatgtcat tttatttgca
      661 ccagaagatc tttctttagt aaaaggcagc ccgcagacca ggcggaagtt tttagatatg
      721 gaattaggcc agatcaatcc aatctatctt tatgatcttg tccaatacca agcaattttg
      781 aaacaaagaa accagtactt gaagcaattg aatgaaaaaa aacaaactga tttgctttat
      841 ttggatattt tgtccgaaca actagcagcg tttggcagca aagtattgaa agcaagagca
      901 cagttcgtca agcgattgga gttttgggct aattctttgc atcagcaaat cacccatcaa
      961 aaagagcagc tggaaatcga gtatctcacc gcagtcgatt ctttagaaac acatacgcaa
     1021 gagcaaatcc aagaacaatt tctcgcactt ttgaaccaaa ataaaaagaa agaccttttt
     1081 cggggaacaa cgacagttgg tcctcatcgt gacgatcttt ccttttttat taatcagaaa
     1141 aatgtgcaga cttatggatc gcaaggacag caaagaacaa ctgcactaag tgtcaaatta
     1201 gcagaaatcg atttgattaa agaagagaca ggggaatatc caatcctttt gttagatgat
     1261 gtgatgagcg agctggatga taatcgtcaa ctccatctgc tagaaacaat cgaaggaaaa
     1321 gttcaaacgt tcttaacaac aacgacactt gaccatgtaa aaaataaaat gactgttgaa
     1381 cctgagattt tttatgttca acaagggaaa ctaaaaggag aagaaactga atgacagaag
     1441 aaagaagttt agtagaacgc gctaaagagt atgatgccag tcagattcaa gtacttgaag
     1501 gacttgaagc cgttcgtaaa cgacctggca tgtatatcgg ttcgactagt tctgagggtc
     1561 tgcatcacct tgtttgggaa attgtcgata attcgatcga tgaagtatta gcaggttttg
     1621 caacgaaaat ccatgtgatt attgaaaaag acaacagtat caccgttatt gacgacggtc
     1681 gggggatacc tgtagatatc caggcaaaaa ctggacgtcc tgcagttgaa acggtcttta
     1741 ctgttttaca tgctggagga aaattcggcg gtggtgggta caaagtttct ggtggacttc
     1801 acggagtagg atcttctgtt gttaacgcgc tttctacaca gcttgatgtc aaagtataca
     1861 aagaaggaaa tgtgtactat caagaatatc gtcgtggagc tgtagtggac gacttaaaga
     1921 ttatcgaaca aacggatcgc catggaacaa cggttcactt tacgccagac cctgaaattt
     1981 ttacagagac aactgagttt gatttttcaa aattggctac ccggatccgt gaacttgctt
     2041 tcttgaaccg cgggatgcgt atatcgattg aagacaaacg agaagaagaa ccagtcatca
     2101 atgaatacca ttatgacggc gggattaaaa gttatgtcga atacttgaat gcaaacaaaa
     2161 ccgtgatttt tccagaacct gtatatttgg aaggtgaaca gcaagatatc gcagttgaag
     2221 tctctatgca atatacagac ggctaccatt ccaatatcat gagttttgcc aacaatatcc
     2281 atacgtatga aggcggaaca catgaatcag gattcaagac tgctttaact cgtgtgataa
     2341 atgattatgc ccgaaaacaa aagctgatga aagaaaatga cgacaacttg accggtgaag
     2401 acgtgcgaga aggattgaca gctgttattt ctatcaagca tcctgaccct caattcgaag
     2461 gacaaacaaa aacaaaattg ggcaattccg aagtacggac agtcactgat cggttattta
     2521 gcgaacattt catgaaattc ttgctggaaa acccaagtgt gggccgtcaa attgttgaaa
     2581 aaggattact agcttccaga gctcgtctag cagcgaaacg tgcgcgtgaa gtaacgagac
     2641 gtaaaggtgc tttagagatt agtaatctgc caggaaaatt agcggactgc tctagcaacg
     2701 atcctgaaaa atgcgaattg ttcatcgtcg aaggagattc ggccggcgga tcagctaagc
     2761 aaggtcgtaa tcgagagttt caagccatct tgcctattcg tgggaaaatc ttgaatgttg
     2821 aaaaagcaag catggacaag atcttggcaa atgaagagat ccggtcttta tttactgcta
     2881 tgggtacagg gtttggagaa gattttgatg tatcgaaagc tcgctaccac aaattagtta
     2941 tcatgactga tgccgatgtc gacggcgcgc atatccgtac attgctgctg actctttttt
     3001 atcgttacat gcgtcctatt gttgaagctg gatacgtcta tattgctcag ccacctttat
     3061 acggggtaaa acaagggaag aatattacgt atgtacaacc aggaaaaaat gcagaagaag
     3121 agttgaaaca agtcgttgct tcacttcctg cctcaccaaa accaagcgtt cagcgttata
     3181 aaggacttgg agaaatggat gatcatcaac tatgggaaac aactatggac ccaagcaatc
     3241 ggatgatggc ccgcgtcagt gtggacgatg ctattgcagc agatcagata tttgaaatgt
     3301 tgatggggga tcgagtagaa ccccgtcgtg cattcatcga ggaaaatgca cactatgtga
     3361 agaacttaga tatttaatca tgaaggagga aaaagaatga gtgaagaaat cagagaaaac
     3421 atccatgatg tcaatctaac cagcgaaatg aaagactcct tcatcgatta tgcgatgagt
     3481 gttattgtcg cacgtgcact tcctgatgtc agagatggtc tgaaacccgt tcatcgccgt
     3541 attttatacg gcatgaacga attaggtgta acaccggata aagcccataa aaaatctgcc
     3601 agaatcgtcg gagatgtcat gggtaaatac catcctcatg gagactatgc gatctatgag
     3661 tcaatggtac ggatggcaca gccattcagc taccgttata tgctagtgga tggtcatgga
     3721 aactttggtt ctgttgacgg tgacggtgcc gccgctatgc gttataccga agcacggatg
     3781 agcaaaatcg ctacagaaat gctgcgtgat atcaataaga acactgttga tttccaaagc
     3841 aactatgatg atacagaaaa agaaccagtt gtactacctg ctcgcttccc gaatctttta
     3901 gtgaacggaa caacggggat cgccgtaggt atggccacga atatcccacc tcataacttg
     3961 tctgaggtag tagcggctat cgatttgctg atggataatc ctgatgtgac gaccaatgaa
     4021 ttgatggaag tattacctgg accagatttt ccaacaggtg gtttggttat gggaaaatca
     4081 ggaatccgaa gagcgtatga aacaggtaag ggttctatta ccgtacgtgc aaaagttgag
     4141 attactgaga tgccaaatgg taaagaacgg atcatcgtga cagaattgcc ttatatggtc
     4201 aataaagcaa aattgatcga acggatttct gaactgcaca gagacaagcg tatcgaaggg
     4261 atcacagatt tgcgggacga atcttcccgt gaaggtatgc ggatcgtcat tgacatccgt
     4321 cgtgacgcca gcgcatctgt tatcttgaat aacttatata aattgacatc cttgcaaaat
     4381 tcatttggat tcaatatgtt ggcgatcgaa aaaggtgtgc cgaaagtttt gagcttgaaa
     4441 caaattcttg aaaactatat cgaacatcag cgcgaagtca tcacaagacg gacagtcttt
     4501 gaaaaagaaa aagcagaagc aagggcacac attttagaag gtttgcgtat tgctttggat
     4561 catatcgatg aaatcattgc gattatccgc gggtctaaat cagatgacga agcaaaagcg
     4621 acgatgatcg aacggttcga tttatccgat cgccagtctc aagctatttt agatatgcgt
     4681 ttacgccgat tgaccggttt ggaacgggaa aaaatcgaaa atgaatacca agaactctta
     4741 aaactgattg aagatttaac tgacattctg gcaagaccag aacgtgttac agaaatcatc
     4801 aagaatgaat tagcagactt gaatcaacga tttggtgata aacgacgcac agaattacta
     4861 gtcggtgagg tcttaagtct ggaagatgaa gatttaatcg aagaagaaga aatcgttatt
     4921 acattgacaa acaatggtta catcaaacgt ttggcaaata atgaattcag agctcaacga
     4981 aggggtggac gtggtgtcca aggaatgggt atccacaatg acgacttcgt caaaaatctg
     5041 gtttcttgtt ctacccatga tacattgtta ttctttacga ataacggaaa agtgtatcgg
     5101 gcaaaaggat acgaaattcc agaatacggg cgcacagcca aaggaatacc aattatcaat
     5161 ttactaggta tcgattcgag tgaaagcatc caggcaatca ttgcggtgac gggagaagca
     5221 gaagaaggcc actatctctt ctttacgacg cgtcaaggta cagtaaaacg tacttctgtc
     5281 actgcttttg ctaacatccg aagtaatggt ctgatcgcaa ttggtttgaa agaagacgat
     5341 gagctcgtca atgtgctact gacagatggt caatctaata tcattattgg aacacataac
     5401 ggctactctg tcactttcaa agaagaagca gtccgcgata tgggacgtac agcttccggg
     5461 gttagaggaa tacgcctgcg tgaggaagat tatgtcgttg gggcagcctt aatgaaggaa
     5521 gatcaagaag tattagtgat cacagaaaaa ggttacggaa aacggacaaa agtatcggaa
     5581 tatccagtga aaggccgcgg aggtaaaggg atcaaaacgg cgaacatcac tgaaaaaaat
     5641 ggtccactag ctggattgac cactgtttct ggagatgaag acatcatgct gattacagat
     5701 aaaggtgtca ttatccgttt caacgtatcg actgtttctc aaactggacg ttcaacatta
     5761 ggcgtacgtt tgatgaagat ggaagctgat acgaaagttg tcacgatggc agcagtcgaa
     5821 ccagaagctg cagaacaaac agttgaagaa caaggatcag aagaatagcc atcaagacta
     5881 attgtgtaag ctagcaagaa tgaccgatta aaaaatctgg ttattcctgc tggctttttt
     5941 gattagttga atttctctta aaatattcga aactgttcat tctgtattgc ttttgaccaa
     6001 aatagagtgt ataatcagag cttgtgagta gtctaattaa ttagataact ctccttgctc
     6061 ttaggaacct aagagccgaa agtccacagg gaggtgaata tcaatggaaa acacgaaata
     6121 tgaaatcatg tacattattc gtccaaacat tgatgaagaa gcaaaaactg ctttaatcga
     6181 acgtttcgat gcaatcttga aagataacgg agcagaagtt atcgaatcaa aagattggga
     6241 aaaacgccgt ttcgcttatg aaatgaacgg ttaccgtgaa ggtatctatc acatcgttaa
     6301 agtatcttct ccatcatcag caaatgctgt caatgaattt gaccgtcttg ctaaaatcaa
     6361 cgacgatatc attcgtcaca tgattgttaa agaagaagct taattgttgt ttcatgtgaa
     6421 acaatagaaa acgatgagag gagatgacta ttttgattaa taatgttgta ttagttggac
     6481 gtctgacgaa agatccagat ttgcgttaca cagcaagtgg tacggcagtc gcaacattta
     6541 ctttggctgt gaatcgtaat tttacaaacc aaagcggcaa tcgcgaggct gattttatca
     6601 actgtgtgat ttggcgcaaa tcagcagaaa ctttagcaaa ctatgctcgt aaaggaacac
     6661 ttttaggtgt tactggacgt atccaaactc gttcttatga taatcagcaa ggacaacgtg
     6721 tatacgtgac agaagtagta gctgacaact tccaattgct ggaaagtcgt tctgcatctg
     6781 aaaatcgcca acaaagcggc ggattccaaa gttctggtca atcagctggc ggatttggcg
     6841 gaaacaataa ctctaatcaa acatctcaat catccaacgg tatgcctgat ttcgaccgtg
     6901 atacttctga cccattcggc tcaagttcaa cgatcgacat ttcagacgat gatttaccat
     6961 tctaaacgaa tttgatagga gggaaataga atggcacaac aaagaagagg cggacgtaaa
     7021 cgtcgtaaag tagactacat tgctgctaac catattgaat atatcgatta taaagatatc
     7081 gaattactaa aaagatttat ttcagaacgc ggtaaaatct tgccacgtcg tgtaactggt
     7141 acaggcgcta aaaaccaacg taaaattact gttgcaatca aacgcgctcg tatcatgggc
     7201 ttgctacctt tcgttagcga agactaataa atcctaggaa ttgaatgact gatccttttg
     7261 atcagctatt caattccttt ttttctgtaa aaataacctc tattcgttta gtcgctcgaa
     7321 tgacgaaagt tatggtaaaa tcaaattagt catatatggg aggaaaatta tggaaaataa
     7381 aaacaatcat ttatccccga aacctctctt ccttatcctg tttatattaa tcgaagtatt
     7441 ggtagtctta attttaccat ttcgttttgt tagcgcttcg gtcattctat tgatgaattt
     7501 atggggtgtt cgctattggt tgaaaatgaa acgttggctt attttaaata aacgtgaaaa
     7561 aatccagcag gcttcgactg tagctgaaac caatctagct tatgtctcag aagtcatgcc
     7621 tgtaggagtg attgcttatc agccatcaag ccaaaaaatc gaatggctca atccattagc
     7681 attgaaaatc aaagatcaaa gtggattaac agaggaagaa ttgctagata ctttttttac
     7741 tgcaaaagaa aaaaagaaaa accaagtcta tctagcagat aaaacatttc agtttgatat
     7801 ggatacagta aagggtgttg tttattttaa tgatattacc caaaaccagt tgttaaaact
     7861 ccatcagacg aacagtcagc cggtgattgg tattttatct attgataact atgcagatgc
     7921 cattgataaa ttggatgaca aagagatttc ttatttgaac tctttcgtca ctacactagt
     7981 ttctgactgg atgaatgaat accatgtctt tttcaaaaga atcaacgcag aacggttttt
     8041 cttcgtagca cagatgatcg atctgaaaaa gatgatggat aaaaaatttg atttgctgga
     8101 tcgctttcga gcagaagctg aaaaacagag ctcgcctatc acccttagta tggggattgc
     8161 ttatggctca agaaccctcg atgaaatcgg tggagaagct cagaataatt tagatgttgc
     8221 tttagtccgt ggaggcgacc aagttgtcgt gaaagaggta aaagaaggag caaaacctgt
     8281 ttattatggg gggaaatctg ctaagaatgt caaacgcacg cgtgttcgtt ctcgagcgat
     8341 gagtaccgct ttgcgtcgga tttttgatga aacaggcgat gtgtttatca tggggcacaa
     8401 gtttccggat atggatgctt taggctctgc ttttggtgtc agttgtctgg cacgcttcaa
     8461 ccagaaaaaa gcttacatcg ttatcaacga gaacgagatc attccagatg tagaacggtg
     8521 tcttgaagaa atccataagc atcctgaatt agaaatgcaa ctaatctctc cagaacgtgc
     8581 catagagctg aaaaaagaag aggacctcct cgtgatggtg gattaccata aaccttctct
     8641 atcgatctct cagggcctat atgagcaatt tgataagatt gtgatcattg atcaccatcg
     8701 tcgtggggaa gagtttccaa gtaaacctct gctgacttat atcgaatctt cagcttcttc
     8761 agcttcagag ctagtttctg agctgatcca ataccaaagc agcaaaacaa atcgcttaga
     8821 tcgttttgag gccacattat tgcttgcagg gatggttgta gatacaaata gtttcaatgt
     8881 ccgtacaact gcacgaactt ttgacgttgc cagctattta aggtcttgtg gagcagacgc
     8941 atccttagta caatatttat tgagctcgga tttagcgaca tacttggaga tcagcaattt
     9001 gattgctgga agcgactatg tgacttctga tattgttgtg gcggcaggga cagacgaaaa
     9061 agagtataat agtgttatag cagcaaaaac agcagataca ttgctttcga tgatcaatat
     9121 caatgcagct ttcgtcatta caaagcggac agacggcttg attgggatca gtgccagaag
     9181 taccggcacg atcaacgtcc aaatcatcat ggaatcctta ggtggcggag gacacttcac
     9241 aaacgcagct actcaactgg aaaatacgac agttgaggaa acaaaacaga agctatttgc
     9301 tgcaatcaaa gaaaatatga atcaaatata tgataaggag taatccaaat gaaagtaatt
     9361 tttttacaag atgtaaaagg aaaaggtaaa aaaggggaca tcaaagaagt tccagcaggt
     9421 tatgcacaaa attatctaat caaaaataac ttggcaaaag aagcaacgaa aagcagtatc
     9481 agtcagttag caggacaaaa gaaagcacaa gaaaaacacg aagctgaaat cttggaagaa
     9541 gctaaaaagt taaaagaatt cttggaaaaa gaagatactg tagtcgaatt gaaggcgaaa
     9601 gcaggggaag acagccgttt gtttggttcg atcccttcaa aacaaattgc tgaagggtta
     9661 aacaaacaat acaaaatcaa attagataaa cgaaaaatcg aattagaaaa tccgatccgt
     9721 tcactaggct acacaaacgt acctgttaag cttcatcatg aggtgacggc tacgatcaaa
     9781 gttcatgtcg tacaagaata acaaatcttg atagcgatgt tcagctggta agtataacca
     9841 gaaggaaaaa agacaaaccg aaatttatct gagaaaacag ctgcagcaat gaaagcaaaa
     9901 gctgcaaatc gatttcagat tgatttcggt ttcttttttg ggaacagcaa aatagcataa
     9961 agaaaacgag tataataaaa gagaactatt tttttattac actttcggta cgaatgatga
    10021 cttttctctc attttcttgt aaaatgaagc aattgaagac aaggagcgat acgtatgaat
    10081 gaagtgtggc aggatcgaat cccaccgcaa aatattgaag ctgaacaagc ggttttaggc
    10141 tctgtttttc ttgacgcaga aacgatcatt gatgccatgg agtacataga gccaaaagac
    10201 ttttatcgtc gtggccatca aattattttt gagacaatga tcgagttaaa tgatcgtaat
    10261 gaagcgattg acgtggttac tgtcaaagac cggctggaac aagccaattt gttagaagat
    10321 gccggtggtt taagttatct ctcagacttg gcattagcag ttccgacagc agcaaatatc
    10381 gtctactatg cgaaaatcgt tgaagaaaaa tcattgctgc gtacattgat ccagacagca
    10441 acaggaatcg ttacgaaagg attcgagcaa ggagaagaag ttcaaacaat tttagatgat
    10501 gccgaacgca gtatcttgga ggtttcggaa aaaagaaatc gaagcggctt tttatctatt
    10561 gctgaagtac tgaactccac gattgaaaat atcgatcgac tagctcaaaa caatgaagaa
    10621 atcacaggtc taccaactgg ctatcaaggt ctggataaaa tgacagcagg tttccagccc
    10681 gaagaactaa tcattcttgc tgcacgtcca gcggtaggta aaacagcgtt tgcattgaat
    10741 attgctcaaa atgtaggaac aaaaacagat cgttctgttg cgatatttag tttggaaatg
    10801 ggggcagaat ctcttgtaaa tcggatgctt tgtgcagaag gttcgattga ggccagccat
    10861 ttacgtacag gtcagctttc ggaagaagaa tggtcgaatc tgatcgttgc gatggggagt
    10921 ctttctcgag caaatatttt tatcgatgat acaccaggga tcaaaatctc tgaaatccgt
    10981 gcgaaatgtc ggaaactagc gcaagaaaaa ggcaatctag gcttgatatt gatcgattat
    11041 ttgcagttga ttgaaggtaa cggtaaagag aaccgtcaac aagaagtatc agatatctct
    11101 cgccaactga aaaaactggc gaaagagctg aaagtacctg tcattgctct ttcacagtta
    11161 tctcgtggag tagaacagcg acaagacaaa cgaccagtgc taagtgatat tcgtgaatcc
    11221 ggatcaattg aacaagatgc ggacatcgta gccttcttgt atcgagatga ttattatgaa
    11281 agagaaggcg gagatgacga tgatggaaac gagccaaaaa atgacaacgt catcgaagtc
    11341 atcatcgaga agaaccgaag cggtgcacga ggaacagttg aactactgtt tatcaaagaa
    11401 tacaataagt tttcgtcgct ttcgccaaga gaagaatttt aaagaagagg ttgtgagcct
    11461 tgagcaatcc cgagagctct cgtaagaaga tgcaaggagt aaccgcagga acgactagag
    11521 tacttaattt aaacagatct ccaaagggat tgtgacaaaa gtcttgtcac agtccctttt
    11581 tccgaataaa cggtgttcaa tcagctgacc cttggtatta gctcagcaaa cgtcaaaata
    11641 tgaggaacta ttttgacgtt tgtttctcta atactcaggt caaaacgctg atcttacgac
    11701 ctcttttcct taaacggtgt tctcctgcca actcctcggt cacaagtcac aatatcatca
    11761 aacatgagaa gcagtttttt gaaattgctc cttgtgactc agagttaaac ggcaggctca
    11821 caacctcttt cttaaacggt gttcaaaagc ttgcatctgc ggtaatacgc ccaatcaaac
    11881 gaaaaatatg agaagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
    11941 ttcacaacct ctgattcacg gtgttccaaa agtagcttct tcgaaaaata agtcgaaatt
    12001 tcacaaa
//
LOCUS       DAZULO010000085        11777 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000085 DAZULO010000000
VERSION     DAZULO010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.087"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            358..627
                     /gene="rpsN"
                     /locus_tag="ACG8OA_002242"
     CDS             358..627
                     /gene="rpsN"
                     /locus_tag="ACG8OA_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS4976992.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            642..821
                     /gene="rpmF"
                     /locus_tag="ACG8OA_002243"
     CDS             642..821
                     /gene="rpmF"
                     /locus_tag="ACG8OA_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS4976993.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            853..1230
                     /locus_tag="ACG8OA_002244"
     CDS             853..1230
                     /locus_tag="ACG8OA_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4976994.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1251..1400
                     /gene="rpmG"
                     /locus_tag="ACG8OA_002245"
     CDS             1251..1400
                     /gene="rpmG"
                     /locus_tag="ACG8OA_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS4976995.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1424..2407
                     /locus_tag="ACG8OA_002246"
     CDS             1424..2407
                     /locus_tag="ACG8OA_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS4976996.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2437..2559
                     /locus_tag="ACG8OA_002247"
     CDS             2437..2559
                     /locus_tag="ACG8OA_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS4976997.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2577..4100
                     /locus_tag="ACG8OA_002248"
     CDS             2577..4100
                     /locus_tag="ACG8OA_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS4976998.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4124..4363
                     /locus_tag="ACG8OA_002249"
     CDS             4124..4363
                     /locus_tag="ACG8OA_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4976999.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4556..5017)
                     /locus_tag="ACG8OA_002250"
     CDS             complement(4556..5017)
                     /locus_tag="ACG8OA_002250"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4977000.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5212..5430)
                     /locus_tag="ACG8OA_002251"
     CDS             complement(5212..5430)
                     /locus_tag="ACG8OA_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4977001.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5548..7164
                     /locus_tag="ACG8OA_002252"
     CDS             5548..7164
                     /locus_tag="ACG8OA_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS4977002.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7405..8745)
                     /locus_tag="ACG8OA_002253"
                     /pseudo
     CDS             complement(7405..8745)
                     /locus_tag="ACG8OA_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9021..9176)
                     /locus_tag="ACG8OA_002254"
     CDS             complement(9021..9176)
                     /locus_tag="ACG8OA_002254"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFS4977003.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9183..9329)
                     /locus_tag="ACG8OA_002255"
     CDS             complement(9183..9329)
                     /locus_tag="ACG8OA_002255"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977004.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9368..9562)
                     /locus_tag="ACG8OA_002256"
     CDS             complement(9368..9562)
                     /locus_tag="ACG8OA_002256"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4977005.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(9472..9804)
                     /locus_tag="ACG8OA_002257"
     CDS             complement(9472..9804)
                     /locus_tag="ACG8OA_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4977006.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10318..10509)
                     /locus_tag="ACG8OA_002258"
     CDS             complement(10318..10509)
                     /locus_tag="ACG8OA_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4977007.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10603..10989)
                     /locus_tag="ACG8OA_002259"
     CDS             complement(10603..10989)
                     /locus_tag="ACG8OA_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4977008.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
BASE COUNT         3931 a         1830 c         2255 g         3761 t
ORIGIN      
        1 gttttaaaaa attggcgata aaccagttaa acataaaaga ttgaataaaa agttatagtt
       61 tctttcatat tgccgtttta agtagaggaa aagctactta aaacggtatt tgtgtttgat
      121 tggactaata aaagtgaatt cttatttttc agagcctaaa ctgcatattc ttttaaatga
      181 tttgattatc tctatgtacc gataaattcg ttttcaaata gttatctatt catatgagtt
      241 ttatttcatg ttatttattg ttgacaagag caactcttgt tggtaaagta tcagtgtcaa
      301 aacgtaacga ttacgatttg aggtgtctaa atcaaaataa aaggagtata aaggtatatg
      361 gcaaaaaagt cgaaaattgc taaagcaaag aaacaaatgg cgatgattga aaagtatgca
      421 gataaacgtc aggaattaaa agccgcagga gatcgcaccg ctctcgctaa attgccaaga
      481 gattcaaatc cgaatcgtct gaggcttcgt gatcagacgg atggtcgtcc aagagggtat
      541 atgcgaaagt tcggtatgtc ccgtattaag tttagagaac ttgcccatca agggttgatt
      601 cctggagtaa aaaaagcaag ttggtaaaag gaggaaatat catggcagta ccagcaagaa
      661 aaacatctaa ggcgaagaaa cggttaagaa gaactcatca aactgtagta aaaccagaaa
      721 tttcatttga cgaagcgaat ggtgattata gacgtagcca tcatgtatct ttaaaagggt
      781 actataatgg aaagaaagta attaaaggag cttcaaaata gtttaatata taacttttag
      841 gagggaatca aaatggaaat tatttaccca cctttagttg aagaaggatt aaagtattat
      901 cttgaaacga cccagcaatc gctagataaa tgcacttttt atcgttcgat ggtagagaga
      961 ggaattatta ctgaaacggg tttaccgacc caacaggcaa ttgaaaatgg attagtcaaa
     1021 gattactatg aggaccaagg cttatcattt gatgattttt taagaatcta tccaattttt
     1081 gaagagtatg acgaggagtt atttcaatgt atcgatggtt attgggagat acctgttgat
     1141 atgaaagaaa atttagtttc acaattggaa tctggcgagt taacttttga agatgcacaa
     1201 caaattcaag catatcttga agatcgataa gtattgaagg aggaagattt atgcgtcaaa
     1261 atattatttt ggaatgtgtc gaaactggag aacgtctgta tctcaccagc aaaaataaac
     1321 ggaataaccc tgaacggtta gaattaaaaa agtattcgcc aaaattgcgc agacgggcaa
     1381 tttttaaaga agtgaaatag ctaagtggta aaaaggtgac aaaatgggca taccagttac
     1441 aatcgtttct gggtttttag gggctggaaa aacaacgcta atcaatcaag ccttgcaagt
     1501 ctcaccctac cccagggaag aaattattat aattgaaaat gagtttggtg aagttgggat
     1561 tgatcacaaa ttattagttc atagcgagga acgggtgtta caactaaata atggatgtat
     1621 gtgttgcagt cttcgcagtg atttattagc aatattgatt tctttactgg aaacaataga
     1681 agaacatcaa caaccaattt cacaaatcat cattgaaact acaggtatag cagatcccca
     1741 accaatcatt caaacacttt taacagcgcc tcaactaaga ggaagagtat atatcgatag
     1801 tttattgact gttattgata gtgacagttt tgaacttatt gaaagagaac cacaagccct
     1861 taagcaactt gtcatggcag atcgcatttt tgtgtctaga aaacaaaact tgaatttatc
     1921 tgcactaaaa aaattacaga gagaaatcaa aagtattaac cctttatccg atattcgaat
     1981 gttcacacat agtgaaacga ttcaaaaaaa ggatttttat aatttgaaaa aatttaatca
     2041 tcccttagtt tttgaaagtg attcatcaga aaatcactat aatggcaaat actatcatca
     2101 acaccatcaa gtacatgagt ttaaatcaat tctcttaact tcagatcaag acataaaaaa
     2161 agatttttta ctccaatgga ttgactggct gattttaacc aaccaaggga gcctgtatcg
     2221 ttttaaagga ttgattgctt tacaagagca ggattttata gttgccatgc aagaggtaaa
     2281 cgaacaggtt aattttcaat acaccactcg acaaaaagac caaaccacta ttattctaat
     2341 cggaaaaatg ttggatgaag aaaggatcaa acagagtttt gataaattac tggcggaaag
     2401 caaataatga taagtagtga ttgggggtaa tttctaatgg agaaaacgga tatatctagt
     2461 gcatatcgac gacttaaaag cccgaatatt aaaactagaa aaagggcatt aaaaattatc
     2521 aaagaacata aacgaaataa aatgaagaaa ttagcataat taaggaagga aaaagaatga
     2581 agaaaatcag tgtaggactt attgggatag cagctctagg attattggga gcttgctcca
     2641 gcactaatga tgcaaaagta tcgaatgata aagatggaaa attagaaatt gtaacaacat
     2701 tctatccaat gtatgatttc accaaaaaca tcgtcggcga tgaagcgaac gtggatttaa
     2761 tggttccagc gggatcagaa cctcatgact atgagccctc agctaaggat atggcaaaag
     2821 ctcatgatgc tgatgttttt gtatatcata acgaaaacat ggaatcatgg gtaccaaaag
     2881 ctaaagaaag ttggaagaaa gctgggccta atgtagtaga aggaacaaaa gacatgattt
     2941 tattgccagg tagtgaagaa gaagatcatg atcacggaga agaagatcac catcatgaac
     3001 tagatcctca tacgtgggtt tcacccaaaa tggcaattaa ggaagtttca aatatcaagg
     3061 atcagttagt taaattgtat ccgaaaaaag caaaagtatt tgagacaaat gctgaaaagt
     3121 atttgactaa actgaaacgg ttggatgctg actataccac tagtcttaaa gaagcgaaac
     3181 aaaaaagttt tgtgacgcaa cacgcagcct ttggatattt agctttggat tatggattga
     3241 aacaagttcc tattgctgga ctaagtcctg aagaagagcc atcttctggt cgattagctg
     3301 aattaaaaga gtatgttaaa aaaaataaga tcaattatat ttactttgaa aagaatgcta
     3361 atgataaaat tgcaaaaact ttggccaacg aagctgggat aaaattggaa gtactgaatc
     3421 ctcttgaaag tcttacaaaa gaacaaatgg ataatggtga agattatgtt tcagtaatgg
     3481 aagacaatct aaaagcgtta gaaaaaacaa ctatggttgc tggtgaagag gtagttccag
     3541 aaaaagaagc gaaggatgaa aagacagttg ctaatgggta ctttaaagat gccgatgtga
     3601 aggatcgcga attgagtgat tatacaggcg aatggcaatc tgtttatccg ttgttaaaag
     3661 atggaatttt ggatgaagtt ttcgattaca aagcaaaaat caataaagac atgacagctg
     3721 ccgagtacaa agattattat actactggct ataagacaga tattgatact atcaacatta
     3781 aagacaacac cattgatttt gtagtcaacg gtgaacatca tcaatacaca tacaaatatg
     3841 ttggatacaa aattttgaat tatgaaaaag gaaaccgtgg tgttcgtttt aattttgaaa
     3901 ctgatgatgc aggtgctggt cgttttaaat atattcaatt tagtgatcat ggaattgcac
     3961 caagtaaggc agagcatttc catattttct ttggtggaga aagtcaagaa aaactctata
     4021 atgaattgca taattggcct actttctatc cagcttcatt gtctaaacat gaaattgctc
     4081 aagaaatgat ggctcattaa aattcaattt ttggaggaaa tacatggtaa gaaaagtatt
     4141 attgtctatg gattccaaga atattgatgc ttatcggcgt tatcgatttg caaagcaaat
     4201 gagattaaat gggagtacat tagctgatat tgctgcaact ttacatgtta gtattgacac
     4261 agttcataga gtattaaaac gggatcctga ttgttgtttt atcaaggtca atactggaag
     4321 aagtagacgt ttagcaaaac ggcataatag cttggccaat taattgataa taccagagta
     4381 gatcattttt cccttatttg gatttgatct gctttttatt atgtcttgaa ttgtcaaatc
     4441 aagcgcaaca ctttatcaaa gaagacttca taaggtgttc tccaatctaa acactttcta
     4501 ggtctgttat tcagtttttc aatcatctga tttatcgttg agttatctac cgtagctatc
     4561 tctttcgatt ttggcaagta ttctcgaatg agtctattcg tattttaatt tgttcattgt
     4621 tgccaagggc atgaggatct ggaaaataaa agggcatatt attcaatgct tctgtaactg
     4681 cgaaatgatt ggagaattct tttcctctat ctggagtgat cgttttgacc gcctcatttg
     4741 gaatactgct taaaagttca atcattttct cgctcacata agcggctttc tttctgggga
     4801 ccttctttcc tgctaaaagg aagcatgatt ttctgtctgt caaagtcact aaacaagtac
     4861 caccagtttt gccagctaca atatcggctt tccaatgacc aaaatcactt ctatcatctg
     4921 cggattttgg acggccatgg atcgaatgag taaggcaaat ctttccacga gactcgacat
     4981 gattttttgt atgtcttgtt tttcctcgat ggtgcaatga acggatcagg cctttattgc
     5041 cgtgtgagag gggttctgta tcgaaaagat cattgcaaat tgcacgttag atcgtgataa
     5101 aactaattga aaagtccata tgttcatgct tcaaacgatt cgagatttcc tctggcgacc
     5161 actgaaaatc gacaaataaa tgacgaacaa ttgaccaaac tcacggatca tttagaatgt
     5221 gataacgtcc gcattttttt cttcgataag cataattttt ttccgctttg gaaggtgaat
     5281 agtttttttc ttgtttcttc tcagttcatg taaaatagta aacgggctat gctgaatgtc
     5341 gttgccaatt atactaatag aaaatccttc ttcgtgaagc agaaagattc tttctcgttc
     5401 gagtatagta agatgacgat actggctcat aattattttc cttctaggag tgttctcgac
     5461 aaacttatac tagttggata tcgattatga ctcattttta tgtgttgcac ttaaagtgta
     5521 aattcaaggt atagaaagag gttccctatg aaaaaaatac ctattacaat cttaactggc
     5581 tatcttggtt caggaaaaac gatacttcta aatcatattc tgtacggtga tcacggaaag
     5641 gaaattgctg tcattgtaaa tgacataggg gaagtaaatg ttgatggggt attagtcgac
     5701 aagaaaggtt ttaaaagaac tggagaaaaa cttgtttcga tgccgaatgg atgcatttgt
     5761 tgtacattac gcgaagattt gattgttgaa ctagatcaat tagtgcaatt ttctgacttg
     5821 aatcaaattg ttattgaagc ttcaggaatc agcaaaccaa ttccaattat tcaagcaatt
     5881 atattagcta aaaacccttt agacattgat ttgacccaac gattagagat tggtgccatt
     5941 attatagtag tggatgtaaa acgtttttgg gataatttcg aaacgggtga aaagattcag
     6001 gaaagaaaag ctgaaggtaa tgtagttgaa gaagaacagg atattcgtgg cttgttaatt
     6061 gatcaattcg agtgttgtaa catattactt ttaaataata gtgacttagt gtcaaccaag
     6121 acgatttaca aaattaacgg tctaattgct aagttacagt ccaatgccaa aataattagg
     6181 acaattaaag gtgaagtaga cttatcgaaa attctaaata caaatctttt taatatagat
     6241 gagacttcct attcagtggg ctggttcaaa gagttagagc ttggctatca aaatcatatt
     6301 cctgaaacag aagaatatgg tatctcaagt tttatctatc gaaatcggtt actccttgac
     6361 gcacaaaaat ttgcccatct tacggataaa aattttcctg gcaatattac aagagcaaaa
     6421 gggtttcttt ggtttgccca acatagtgat ttttcgacga tctttgaaac atcgggtagc
     6481 tataaaagta ttgaatcatt aaattattgg gtagctagtt tacctaaaat tgagtgtcaa
     6541 atgattatac aaaaaaatcc cgaattgaag gaagcctggc atccgttgtt tggtgaacgc
     6601 attaataaac tagtttttat tggtatgaag atggatcaac aatgtattaa ggaacaactt
     6661 gagggttgtt taactgattc tcttaatgca gataatttcc gaaaaatttc accatttcca
     6721 tggaaaaagt ctaatatgtt tagccagata tcgttaatga aatttttaag aaaagctggt
     6781 gtcaagatag gcgatgtcat aattattggc attttgatta ttacctcttt tattccctta
     6841 tttgtattct ctatagaaaa ggagaatgaa acaactgaag atgctatcaa atatgccatc
     6901 gttcgaattg atggcaagga aatggatcgc ttcgatttgg ataagatcaa tcataaactc
     6961 gtgaattatc atcctgcgaa gggacaatat aatattgttg agattaaaga tggaagaatt
     7021 cgagttaaag aagataatag tcctgatcaa atcgcggtta gaactggatg gatctcaaaa
     7081 cccggtcaaa cgagtatttg tttaccccat aagttagtta ttagtattga gaaaaaagaa
     7141 agcacagatt actacattta ctaatctgga aatagatgag gaggttttta ataagaaaaa
     7201 gatattatta ttaataatag caggggcttt attaagtgtg ttaagcgctt gtggacaaga
     7261 aaaagaagcg gtgacaacag gcacaagtat ctttaacggg acctgcaatg gtttagttaa
     7321 cagtcggtta gattacttat tttggctctt cgtcaatgag tgttggtgaa gaaattcaat
     7381 agaataatcg agatactcac acggctagac agctatcttg tctagccgtg ttgttgttct
     7441 ggctttagtc agttgggtag tattcttagt tttcttaaaa tattttcctc gatataaaaa
     7501 gattcgttct cggtagtttt gaaagtttct atagccatag gcaattcgtt ttagtacttt
     7561 gatatggtta ttcaaacatt ctatagggcc gttggaatag ggaacgcgca acgctcgttt
     7621 gatttctgtt tggtatttct tgaaggtcgt gaacactggt tggtagtgct ctggcaactc
     7681 cttgtagtct tcttttagga gcgcgacgaa ttcaggaaca tcttgtcttc tgataacgga
     7741 aagaaaatcc tggtagactt cgtagcctcg ctttaaggaa tcatcataag ataatagccg
     7801 atcgactatc tctgcttctg tcagatgatc tcgaaatgac ggacgccagt agcgatgctg
     7861 acctttcaat tcccacgcat tcttttgaag tagtttccag tatttcttta agtgcttcga
     7921 ccgacgatcc ccttttttaa aggtcttcat tacttgaatt cggtggttgg taaaagcccg
     7981 accaatgtgt tggctaatat gaaagcgatc ggcgacgatt cgggcattcg gaaaacactc
     8041 tttcaccaat gtgcgatagg gtgtatagaa atctgaaacc actagacgca cttgttcacg
     8101 gacttttata gggtagcgca agaaatagtc gcgtaaagga ttgagttgcc gattttccac
     8161 aatatcaatc agttgatggg tcttgccgtc catcatgaca aagctcatgg ctcccgagac
     8221 atttttcact gatttgaatt catcgaaaca caccacttct ggtaacagct ggctcaaaga
     8281 aatggtttta ggccgaaggc tgcgaagtat tcgaatgaca gtggtcggag aaatgtgctt
     8341 catacgtgcg atcaaagaca tagaaagagg ttcgcttaat agctcaagaa tggcttgttt
     8401 gactctgcga gcaatggagc agcgacgatc actaactgaa tcttctgcta aaaaagtctt
     8461 tccacagtgt ttacatttga agcgctgttt gttaatccgc agaatggtct tgtagttact
     8521 gacatcattc aataagatgg atactttttt ccagccctat ttaatgatcg tttgttcatt
     8581 ttgatagtgg cgatgaaagc attcctccgg ttgataggtc aatgtgccat caaaaacaaa
     8641 acatgtttcg tcattgattc gctctttgcg aaaggcatct tcatgaaaga taagatttag
     8701 gtctagaata tctagtaact ctttggtaaa ctctgaatag gacatcttga aacactcctt
     8761 tgaatggttt tcgtcgactt tattctaaag gattttaagg tgtctgtttg cgtttattag
     8821 aaaaaagaaa aagtattggc aacagaaggc gtcaccaaca ctagatatta tagatccctt
     8881 attttcttcg cttcaaattg tcaaggagtc atcataaatt ttgtatggat tcttttgttt
     8941 gggtagtagt cttctaaata atcttggatt aacaccttca tctctatttt acttttatat
     9001 ttgttggagt cgattaattc tcatttgatt gatcgatgaa acgcttcaat tactgcattg
     9061 tcaacaggag tcccagtttg gctcactgaa tgtgtgatgt ggtggtcgtt caacagctct
     9121 tgataaataa aagaccgatg ctggctacct ctatcggaat gaattaccgt aggcaattct
     9181 ccttaataaa tttgtaatgg acggacgaca atctcttttt tcatgtgtga atctaccata
     9241 tagtaacgta gaatacgagt tgctaagtca atataggtgg acaaatagag gcgaccgtca
     9301 ttacatggaa tgtaagtaat atccatcacc caaacagcat caatttggtt ctgttcaaat
     9361 tatcgattca acattttttt accatttctt tgattgtttt ttctctacca tgagaagatt
     9421 tgcgtcgatg aaatcctctt gtgcataaat tcatttcacg cataagaaca gctacaacac
     9481 gtttattgat ttcgattcct tcatcgtaaa gtttttgagt gatccctgga gatccgtatc
     9541 gatctttgtg ttcgtagaac actcgtcttc ctcttttagt cgtttatttt cctgttcaag
     9601 aatgtagaaa tctgctttat caggtaatct ttttccttgt ccagcaaaag catttttccc
     9661 gtatcgacga tattctgaaa cccatcggga aattgttgga cgagagatat tgtattcttc
     9721 agcaattttc ttggtagtta cttttttctc aaggatatcc tgtgaaatct ttagtttaaa
     9781 tttacgatca tatgtttttc tcaatagagt tcattccttc cggtaacgta actgatagtc
     9841 aagaaaagtg tatcatagcc caacttagta tcagggtttg tcaactaaac tgtggaagtt
     9901 gatagataag agtttttaac aaccaaaatt ctcttgacta tttttgaaat cagacaaatt
     9961 ttgattggac acatagttga ataaatctac cgtcgttacg gaaggtaaat cgcatgcttt
    10021 tcaattctag aggagaagtc ctcgttgaag taccatagcc cagttctgga ttcgacctcc
    10081 tgcccacttc atacgtaaat aaagtaattt taggagtgag actgtgacaa aagttatttt
    10141 taagctaaaa acgaattaaa aaacgttgat aattaaaatc attcgtttta gattctatga
    10201 atcattatag aacgctgtaa aatcgatact ttataattat aaagaagata gtttcaaatt
    10261 tattgttcga aatttgaaac tatcttttta cttatgtcac agactccctt agttgagtta
    10321 ttctttaatt tcgaatttcg agatagctag atatattgca agcatacatt cttgataaca
    10381 atcttgatca tattttccag ttctccagga ttgcttatga atcaaagagc taaatctttt
    10441 gagaattgcc tcaacagctt cttcatttcc agcctttgct aaaatgaaat cttcttttaa
    10501 agaattcata aatctacctt cttcccatgg acttataatc tatttctttg ggaaggagga
    10561 gaacgagcaa agaagcatac aaaattagtt attatgcaga tattaaacca gcattttttg
    10621 aatttttttg taaagtcgat gttttaaatt tgtcgcaccc tgtcttgtaa tccccagaac
    10681 ctttccaatt tctttatcag ttttatcaaa gataaatttt tcgataagaa aaatttgttc
    10741 aggttctttc aacaaaggaa ggatttttgc taattttata tcttcaacaa ggagagtcac
    10801 atttaaaaca ttaatcggtt gtttaaattt tatagtatct ttagtaaaca aatatggtgg
    10861 gagttgatca agttgttcat gtttttgaat ttcttcttgt tttttataat aagtattagc
    10921 ggtgttcaaa atgacctttt caaagaatcg aatcaactgg gcatttacgc gttcgtcgtt
    10981 atctgacata ttgcacacct cctccattaa gatttaaagg gaaaagaggc aaaaaacacg
    11041 aaaagaagct aatgcaatta tgctgcgctg cgaataactt ctataagctc acatgcattg
    11101 ataggactgc tgatgtttat tttcaaaaat gtttttttag ttgataatgg atagttatct
    11161 ttatacttag aaaagaaaat tcatatatct tcttaaatat caaacatgtc cttatggcta
    11221 agaaaatgtc ttgagctttt tacgtataat aaatcttttc taaaacctcc ttttggataa
    11281 tattcttatt cgtgtataga ttgtattttt tatttcttcc cccttcataa ttaacaacta
    11341 gaaaagagtt cgaatagaca cgtatttgaa cgaaaatttt tattggattc ccaaattgga
    11401 atatttagaa acgaaaaatt cctattatct gtatagtgct tagataataa cggatcataa
    11461 taattattgt ctttgaacta gatatatgta aatagtattc atcatgatga aaaatgatga
    11521 gttctcggtg ataaaatgta ctttttacgg tggaatatac atgaaagcga taagtggata
    11581 ttttataatg aagtaagtta gcttattttg acagaggaat agtattttag ataacgtata
    11641 aaatactatt taatgatgta gaaaggaaag agagaaacaa aagtgttttc agataaagaa
    11701 gtcttattaa agtctcttca tgtttgttta aagttaccta tcgtggtttt taattccgaa
    11761 ttcagtatta taaaaga
//
LOCUS       DAZULO010000086        11522 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000086 DAZULO010000000
VERSION     DAZULO010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.088"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(124..669)
                     /locus_tag="ACG8OA_002260"
     CDS             complement(124..669)
                     /locus_tag="ACG8OA_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS4977009.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(681..1373)
                     /locus_tag="ACG8OA_002261"
     CDS             complement(681..1373)
                     /locus_tag="ACG8OA_002261"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFS4977010.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1392..1676)
                     /gene="macP"
                     /locus_tag="ACG8OA_002262"
     CDS             complement(1392..1676)
                     /gene="macP"
                     /locus_tag="ACG8OA_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFS4977011.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1689..2252)
                     /locus_tag="ACG8OA_002263"
     CDS             complement(1689..2252)
                     /locus_tag="ACG8OA_002263"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS4977012.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2480..3142
                     /locus_tag="ACG8OA_002264"
     CDS             2480..3142
                     /locus_tag="ACG8OA_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFS4977013.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3266..4462
                     /locus_tag="ACG8OA_002265"
     CDS             3266..4462
                     /locus_tag="ACG8OA_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFS4977014.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4563..5939)
                     /locus_tag="ACG8OA_002266"
     CDS             complement(4563..5939)
                     /locus_tag="ACG8OA_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4977015.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5976..6842)
                     /locus_tag="ACG8OA_002267"
     CDS             complement(5976..6842)
                     /locus_tag="ACG8OA_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS4977016.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6931..7956)
                     /locus_tag="ACG8OA_002268"
     CDS             complement(6931..7956)
                     /locus_tag="ACG8OA_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4977017.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7974..8648)
                     /locus_tag="ACG8OA_002269"
     CDS             complement(7974..8648)
                     /locus_tag="ACG8OA_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4977018.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8832..9725)
                     /locus_tag="ACG8OA_002270"
     CDS             complement(8832..9725)
                     /locus_tag="ACG8OA_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4977019.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9799..11496)
                     /locus_tag="ACG8OA_002271"
     CDS             complement(9799..11496)
                     /locus_tag="ACG8OA_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4977020.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3422 a         2523 c         1974 g         3603 t
ORIGIN      
        1 taacgtaaaa agaataagtt tttttaaaat aacgtataaa aaagagcata aaacaaagtc
       61 gtttgtttta tgctcttctt ttattatttt gtgcttttta ttttatcaaa tcactttgtt
      121 tttttataaa atttctgcgc ctagtgtgga ctcaaaatgt ctcaatgccc attcatgtcc
      181 aacgggatcg aaacttgcca cggcatcttt gaaaatatgg agtttgtatc ctagattata
      241 cgcatcgact gctgtatgca acacacagat atctgtacat acgccggtca aataaatatc
      301 tgtgatctgg cgttctcgca aacggatgtc tagatctgtt ccactaaaag cggaataatg
      361 ccgtttgtct atccaataaa cattttcctc ttgctcatag tcttggtata aactttttaa
      421 agagccgtac aattcccgtc cacccgttcc tatcacatta tgcggaggaa ataagcggtt
      481 ctccggatga aaggaatctt ttggatcatg cgcatcaatg gcaaaaacga caaaatcctt
      541 ttgatcaata aacttctttg tatgagcagt cagctttttt tcaattactt gtcctgctgt
      601 cccagtagtc agctttccat cctctgcaac aaaatcatat gtgtagtcaa tcgagatcaa
      661 tgcgttcatc ttagtcctcc ttatacgaga tgtttgacaa attcgatcac catttctgct
      721 gatcgtttgc ctgcgtcttc gataaattcg tcaaagcttt gtgtcgccga gtgatctgct
      781 gtgtcgctca ttgcacgtac gatcaaaaat gggatattga actgacgagc tgtttgtcca
      841 acagcggctc cttccatttc gcaagctaat gcttcaggga aattcgttaa gatttctttg
      901 attttatctg gcgagtcgac aaatgtatca cctgtaacga tcaatccttc ttttgcgttc
      961 aaattcgttg cttttgctgc tttaaccatt tcactgcgca aatatgtgct tgcttcataa
     1021 tatagaggca ttcctggtaa ttgtcctggt ttgtagccaa atcctgttgc atctgcatca
     1081 aaataagcta ctttgtctga tataacgatg tcacctactt gtaagccttc accgattccg
     1141 ccagctgaac ctgtattgat caccatgttc acgccgtatt gttggatcaa cagacttgtg
     1201 gtaatagatg ctaagacttt tcctataccg gaacgtacga cgatcacttc gtggttacct
     1261 aatgaacctg aaataaataa agcgcctgcg cgttcccatg ataatggttc gcttaggttc
     1321 tctctcaaaa tttttacttc ttcttccatt gcgccgataa taccgatttt catcttttta
     1381 attccttctt tctatataaa tgcgattgct aaaaaaacca cacataacat taggataaca
     1441 ataataagag atttcatcaa aaaagagttc catttggtcg tcttttcccg ttcgctgatc
     1501 cttgttttcg tgattggttt attctttttg ctttctttac gataaaagct ggcaatcttt
     1561 ttttcttctt gctgataagc tgtttctgct tttcgctgtt tttttaatga ctcgcttgct
     1621 tgtgcttgtt gtcgcttgcg aagttcgctt cgcgtaataa gcggtccttt ggccataagc
     1681 cgatccctct actttccttt ggtcaatagt tcccagtatt gaatcgcata aatcgtcttt
     1741 gcatcacaaa tcaactgatc tttcatcgct tgttgcgcct cttcaagagt tagatgatac
     1801 aactccagta cttcgtcttc atcttgtgct aaaggattct cgaccttctc taccccttgt
     1861 gcatgataaa tatgaaggac ttcatttgca aaacctggtg aaagatacat cgaagtaaga
     1921 tggctcagcg acttcgcacg atagccagtt tcttcttcta attctctagc tgctgtcatt
     1981 tcgggatttt gtccttctcc tggatcgatt tttccagctg gaatctccaa aatcactttt
     2041 tctaaaggtt ttcgaaattg tttgacaaga agcagacgat cttgttcgtc aaaagcaatg
     2101 atcccgacac caccaggatg aaataccagt tctctcttcg aagttcctcc atctggcaaa
     2161 cgaacttcgt ctacagcaac atcaataatt tttccgctat agatttcttt gcgactgatt
     2221 gttttttctt caaattgctc attgttcagc atatcttctc ctgctttctt tgtaatcata
     2281 actgcttttt tttcattttt caatttttct caaaaactcc ttattagttt accatagata
     2341 aaccttagat tttctctaaa tatagaaaat ccttctttag tccgatactc tttctagtag
     2401 tccgtatgct aaaataagta tgtaatcgaa taacgaaaag aattcaatac tcaacgataa
     2461 atgacatgga gggtttatta tgataaaacg aaattggccg tggcttctag cattggtctt
     2521 gctatttctc tttgtccgaa acaatagttt ttcttttgtt ctaggacttt tattgatagg
     2581 tgtagtttta gttttgtggg gactctttgg ttcgcgcagg aaaaagggaa aacaagaaga
     2641 aattcctgca ctgactgacg aacttgaaac tcactatgaa aaaagcggta tgacagcgaa
     2701 tgaaatttca ttttttcgac aaacaatgaa tcaaacaaaa aatgagatca atcaattgca
     2761 aatgaatatg cagcagacag ccaaacttaa agcaatcgat ctccgccatg atccagtcaa
     2821 agcagcgaaa gctttattca aagaattggt gaaagaacca aaccgactgc atgaagcaag
     2881 tcagtttctt tatacgcatc tgccgaatct agttgatttg acaaataagt ataatgagat
     2941 caatgaccat gagatcaaaa acaaacagac ctatgaaaaa ctagaagaaa gcgcgcagat
     3001 catcgaccag ctgtcagctt taatcgcaca agattatcaa aaattcgttg ctgaggatct
     3061 agatgatatc gacgtagaga tatctgttgc gaaacagagc ttaaagcgtg ataataaata
     3121 tgaaccagaa caaaaggatt aactcttttg ttcataaaag aagactcgga acattcagat
     3181 gatgtctggc tgtttcgagt actttttcta aagtagagac attcatgtaa gaatcgagaa
     3241 cagattcaac aaggaggaat tttttatgga tcataaaccg actgaaacaa aagatgttac
     3301 accagttaac gatacacttg aagatttatt gaacaacccg ttttcaactc caacagatac
     3361 gctgaccact tcacaacaag cggagattga tcagctccaa gagcaacaga cagctgctcg
     3421 tttaattgac aaactaccgg cggaacgcca agaacaagct aaacagctag cagcaaaaat
     3481 cgatgctaat gatgcacagt ctgtcatcag ctacggatcg gctgcccaag caaaattaag
     3541 tgaattctca caatcgatgt tgaatcatgt gcaagcacaa gatattggtc ctgtaggaga
     3601 ttccttaact gaactgatgt atcgtctcca agaagccaat cctgacgaac tgcgtgccgg
     3661 agaagggaac atcttccagc gtgtcttcgg gaaggtcaaa caatcgatct atgaagtgac
     3721 agcaaaatat caaaagattg gtgcacaaat tgacaagatt tcagtgaaat tagataaaga
     3781 aaaagacggt ctgctaaaag ataatctgat gttagaacag ctttaccaga aaaacaaaga
     3841 ctactttgat gcactgaata tttatatcgc tgctggagaa ttgaaaatgg aagaacttca
     3901 aacaacgatc attccagaag cgatgaaaag agcagaagaa actggcgatc agatggatgt
     3961 tcagattgcc aatgattaca cgcaattcct agatcgtctg gataaacgta cgcatgatct
     4021 tcgcttagct agacagatca cgatccagca agcaccacaa atccgtttga tccaaaacac
     4081 aaatcaggcc ttggctgaaa agatccaagc atctatcgct acagccattc cgctgtggaa
     4141 aaaccaagtc gtgatcgcat tgaccttgct tcgtcaaaaa gatgcagtaa ctgctcaacg
     4201 ccaagtctct gaaacaacaa acgacttatt gaagaaaaat tcagaaatgc tgaaaatctc
     4261 agccatcgaa acagcgaaag aaaatgaacg tggaatcgtg gatatcgaga cccttcaaac
     4321 cactcaaaat gatttgatcg aaacaatcca agagacattg cgtatccaaa aagaaggaaa
     4381 agaaaaacgt cgtcatgcag aagtagagct tggacacatg gaagaagatc tgaaacaaaa
     4441 actgttggat ttaacccaat agatcacctt tcgcttgtta ctaaaacaaa cgcccaacaa
     4501 cagtttatta caactgttga tgggcgtttt tcttatcttt cttctaactt ctccattgga
     4561 gtctatttct cattcatctt gctgtgtcca tagccatagc caaaataaat gatcagtcca
     4621 ataaccatta cgataccgaa cacgatccat gtcacagttt gcagacgaag catcaataca
     4681 atacaagtca agatggagac gattggcaat agcggaacga atggcacttt gaattctcca
     4741 gcttttggtt tgccaaacat ttttctcaac ttcagtaagc ctagactaac aagcatcaaa
     4801 taggcgagcg ccatgatatt cgttagttcc gctaatacat tcaatgggaa aaatccggca
     4861 aagaatacag ctgccacacc agcggcataa gtcgcttttt tcggtgtacg atgcttttga
     4921 tcgatttctg tcataaactt aggaagtaag ccatccttgc tgacagcaaa cagtaaacga
     4981 gcaagtgaat acatcatcga gatcgttacc gtcagtagcg ttaaaattgc ccctacagcg
     5041 atcaccgcgc ccactgttcc atgtcctaca tacctcattg caaaagccac tgggtcattg
     5101 actcctagtt cagtatacgg aacgattcct gtcaagatca gcgtcacgat gatgtaaagg
     5161 acagtagcaa tcagtatcga accaatgatt cctcttggca catctttttg cgggtttttg
     5221 acttcctcgg ctgccattgc aacggcatca aatcccaaga aagcaaaaaa cacaagagct
     5281 gcaccatcta gcacaccgga aaaaccaaaa ggcatgaatg gctgccagtt atcaggttta
     5341 acgaaaaata ttcctacaat gataaacaac gcgatgatac caaatttgac atataccatg
     5401 gcattattca aacgtaatgc tttcttggct tcaagcgaca cgatgtaagt gacgaatatc
     5461 actacgagca ccgcaatcaa gtcaatgtat gtaccatttt ccggattgta cccgccagtt
     5521 aatgcttgtg gaagttcggc ccctaaactt ttcaaaaaac cttgcatata gccagaccat
     5581 ccggaagcaa cagaggaaac cgctagtaaa aattccccga tcaacaacca ccctgtcagc
     5641 caggcagcaa attcaccaaa tacaacatac agataggcat atggcccacc aatcactggc
     5701 actcgcgaag caaattctgc gaaacttagt ccagacaaaa tgacaacgat tgcagctaag
     5761 acaaatgaaa gtgacagtgc tggaccagca ttttgattgg ccgcaacccc tgtcaccaca
     5821 aatattcccg taccgatgat cgcaccaatt ccaagcatga tcaaatcaga cgtttttagt
     5881 tcttttttca tctcactcgt atgattgaga ttgatctctt tcttacgaaa caaatccatt
     5941 gaagttcctc cccattttag gttggaaaac agcgattaaa ctgttttcat ataccctaaa
     6001 aggataccac ctaaaatgac taaaaggcaa ccaacaataa cataaatgag ctcttttttc
     6061 gtttttcttt cgccaaggat gaaaatccca cctaaagtag agataatgat ccccatttgt
     6121 gaaagtgaga agccaacagc taaaccaagt gatttgactg ttagaagcat gcagatgttt
     6181 cctaagcccc ataataatcc acagatcatg tttttccaca caaactgatc gactttaact
     6241 ttacggaaag caaagaagct tgcgcctaag atcattccga tactttgagg taagataatt
     6301 gacatcgcat ccaaattcgc ccagttgacg atgatggtat atacaccata accaatcgta
     6361 gagaagatca atgctctaaa acccttacca aaatcaagca ttttattatc tgtatctatt
     6421 tttccttcgt catcttgtcg ggcagtcaaa tacgcaccgc ttacaagaag agctaaagca
     6481 ataataccgt atacataatc tttcgtttgt gtccattcat ggaagaaaac ggctcccgca
     6541 atcgtattca aaattaattg aaaaccagta gacaatggta gccctacaga aacacccatg
     6601 tacttcattc catggaactg tccattttgt ccaacactcc aagctaagcc ggataaaaat
     6661 ccgatcaaga aaatccaagg ggtgattaca ggactaacaa cgaaaaagac aactaaagaa
     6721 aacagaaatg caccgatcgt cattcctagt gtttgttgat tggcatcgcc accaatttta
     6781 ccacttacta aaccaatact accccatgcc aacataggga ctaatgctat taaaatttcc
     6841 ataaccaatc tccttttatt tcacatgata agaaacttat cagcgaaaac gttaacaaca
     6901 caagcacttt ttttcgtttc taacgatttt ctaacgtttt tcattataaa aatttaagat
     6961 tgggaaaaat aaagtcattg ttgtcccttt tccttcagtt gattcagccg tcaaatggat
     7021 acctaatttt tcggccagat tttttgctaa ataaagtcct aatcctgttg aatgtgtttt
     7081 gctaagtcga ccatttctcc cagtaaatcc cttatcaaag atccgtttca agtcttccgc
     7141 tgggataccg atccctgaat ctttaactga aaggctgact ccttgcgctt ccttttctat
     7201 gctcacaatg atgtttccgt gatccggcgt atatttgatg gcattactaa gcaattggtt
     7261 gaagataaaa gcgatccatt tagcatcggt caaaacagta tgatcctcac caacgatctc
     7321 gtagcgaata tttttttgga taaaataatt tgcctgactg cgtatcacag gctgaatgat
     7381 tccttttagt ccatattctt ggatgaggta gtcccgcgag aaactgtcga gtcttgcata
     7441 atacaaaact tgttctacgt attcatcgat ttttgataat tcattttcta gtaatgtata
     7501 cttttgatcg tcaatatcga actcgatcga acgaactagc aatttagaag cggaaagtgg
     7561 cactttgatt tcatggaccc atgagtcaat ataatctttt tggtcttgtt gattactgat
     7621 tgctgattgc attacttctt ggtgttcacg gatcagctga ttgatatatt cttctaccag
     7681 tttttcttct tctgtcctcc caccactcaa ataattttgc aaaggggaag ttttttgtat
     7741 gtcggctaat tttccccacc atcttctctt gctcaagtaa ccacccagca gataaaaaat
     7801 cagaaagacg ccctcaatca aagctaaata agcaattgtg ctcaaatcaa ctttgatgtt
     7861 tggcgccagc cacatcacaa agatcgtcaa tgcaataaaa aacagccatc ccaagatcaa
     7921 tgtccactga tctttcaaat atttaaaaaa attcatctta accgctcatc ctttcaggga
     7981 acgatatatc cctgtcctac ttttgtttcg atatatcctt ggatacctgc ctgttcgatt
     8041 tttcggcgta accgattgat gttgactgtc aatgtattgt catctacaaa tcgttcgtca
     8101 tcccacagtg ctcgcagcaa tttttcccga cttaagatct ttccattgtg cttcatcaaa
     8161 ataaataata gacgatattc atttttgctc aaatcaatga tctctccgtt gatttccata
     8221 cttccgttgt caacattcaa cgtaatgcca ttgtggctca tcatttcact agataattct
     8281 gtgtagttgt aagaacggcg aagtaatgca ttgatttttg caatcagtac atcgatttgg
     8341 aaatgttttg tcacaaaatc atctgccccc atgttcatag ccatgatcat atccatattc
     8401 gtattcctgc tggagataaa aatgatcggt actttcgata cttcacgtat tttttgacac
     8461 caataatagc catccaaaac gggaagatta atatctagta atacaagctg tggttcttct
     8521 ctttcaaagt ctgagaacac ttgattgaaa tcggtcgttc caaaggtttc aaattgccat
     8581 ttctcaagtt cctccatgat caattgtcga atgatctctt cgtcttcgac taccatgatt
     8641 tttgccataa aaaatccctc catctttctt cttgctacta caatatcaaa gaagcccttg
     8701 tgaaacaagt tggcttacct ggaaaatgtc attttagcaa acgttaaaaa aaactggaaa
     8761 tatgcgattt aatcgcgttt gtctcttcca agcagcttaa atttattatg ttattcttaa
     8821 taattggtct attatctgtt atttttcttt cgttgttttt gtcttttaaa ttttcgataa
     8881 caccaataac cgaaaagcgt tagcactagc aatataccta gaaggagtat aagtaatcga
     8941 taaagatggt atttttttgt ctgttctatc tttgaagaga cttcttctgt aaagggaact
     9001 cgtcttcctg tcactagtaa tcggtgagtg ttcacggtgt aaggtgtaca agtgataagt
     9061 gtgactagat cctgcttttc tctaattgct aatgtatttg tttttgttgg aaggacaaca
     9121 attttctcaa cgacttcata agcgagcatc cgaccataca cttcaagata gaatcggtca
     9181 tcttgctcca tttcttcaag atccgtaaag agttttttct cagcaatacc gctatgggcg
     9241 gtaatcacgg aatgtgtact ctctcctcca gttggaaaag aggtgccttg caggactgtc
     9301 gctcctctat ctaacaaaag atcattcgtt tcatcaaata aaggcaagct aacattgatt
     9361 ttcggaatat agatggctcc gatcatatgt tgttcgtaat attcttttcc tggattttgg
     9421 acatttctaa ccgcttgctc aaaaggatct ttgacttgtc ccattcctgg gattactgat
     9481 gtatgagcaa gcgtctgatt tttcttttca tgcactgcta attgttgctc atgtttttct
     9541 tctttatcgc gttcaagctg cttttgataa ttctcgatcc ttctttgatc caaataattg
     9601 ctgagagcat ctgctaaaaa tggatatgta aaaactgcag ctccggcaag aaacatcact
     9661 gccataaata attgtaataa aagactgatt tgggtttgag gtctattacc tattttcttt
     9721 ttcattttct tcaccttctt atttaaaaaa actgcagcaa cctttataaa accgattgct
     9781 gcagcttatt tttgttgctt atacttcaga cttcatttta gatttacggt accagctgta
     9841 cgctccaatc atcaaactta ttccgattag taagaatgcc aagagcccgt tcccaccagt
     9901 agcaggaagt gacccgcgtt tcatattcgg tactctttct ggccctggat tttctactcc
     9961 tacgatctga atttcttcat tatagcttcc ataagagatg ataaattcag taaaggctgc
    10021 ctcacctgtt ggaagtacat aaccttctgg cgccatggtc tctttcattt gataggttcc
    10081 ataatctaac ccttgaacct caaagtatcc atttccatct gcgacaaatt ctgttgcttg
    10141 tccgcttgtt gcccactcaa ttctatctgc tcctgattca taaacagcta aaggcgactc
    10201 ccctttataa aatattgcgt attcagaacg gtcttcattc cacaattcga atcttgcatc
    10261 tttaagcaat tcactgcttg atgcatcata tttttcaaac tttcttccac ctgttgtgac
    10321 tttctctggc ggctctactc cttcttcata atcaaattca tcatttcgtc cgatttcaat
    10381 aaccgctgtg ttgttatgga aatcatccgc ttctagttct gtggaaacct tcaaattata
    10441 cgtgatcgtt aaatctcctc cacgtgcatc tgctaatcta cctaaagtat ctactttggc
    10501 tactccattt tcttctgtca aaatcgtcac gacaaacccg ttgcttacta cttctactat
    10561 ataatcttgt ccttttgtta aaatcgtctc gccggctcgc acttctattt cctgatcata
    10621 ataagttaga ccaactgtag gcgtatctgt gattttgtaa tttttgataa cagcgcttcc
    10681 gttttcaagg acagaatcga tgaaataagg aataggtgct gtgatcgtgt aagaagccgt
    10741 ttttcctctt tctagatcaa tataagaaat cgtttcttgt tctccagctt cattaataat
    10801 cgttacttgt ctaaggtcgc tttcctctac attcatctgt ttatctgctt ctctgatttc
    10861 gtttttcgga tacaaatgca catctgtatt gtatgtgtct ttccatgtag aaccatccca
    10921 cacgccatcc accatatcag gcatcatcac tggcatcata acaaccattg gagacgcgat
    10981 tctggaaata tgagctggcg aataagtttc taagaaaaga tagactttat ctctagaaga
    11041 aggtgcttct tccgtcactt ggacggtaga gaataccgct tctccagctt cattcgttgt
    11101 ttgtttgtca atacttgtca gtcgatattt taaaaaccaa ctttctgtgt gccgtttttg
    11161 aatccattca atcgcttcgg cagaattaag accgtcttct cgttccggtg ttaaaggatt
    11221 atctcgaatc aaatcgtaat atacttccgt tacgtcaaac aaatcaaaat cgactcctgg
    11281 aatattttct tcaccaaaat ccgtagaaac caatccgcta ttcggtcgtt cttccggaac
    11341 agatgagaaa ccttttttgt gtaatgtaat cgtcacttca cttggttttt gtgcgacttc
    11401 ttcggctttg actagttttc cgttcccgaa aatccctatt cccgcagtaa cgagtaacag
    11461 tacactcatt cccgtgacaa tcattttttt attcattttt ttctccccgt tcttttttta
    11521 tt
//
LOCUS       DAZULO010000087        10877 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000087 DAZULO010000000
VERSION     DAZULO010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.089"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..281)
                     /locus_tag="ACG8OA_002272"
     CDS             complement(<1..281)
                     /locus_tag="ACG8OA_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4977021.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEA"
     gene            453..1541
                     /locus_tag="ACG8OA_002273"
     CDS             453..1541
                     /locus_tag="ACG8OA_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4977022.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1669..3045
                     /locus_tag="ACG8OA_002274"
     CDS             1669..3045
                     /locus_tag="ACG8OA_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS4977023.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3446..5026
                     /locus_tag="ACG8OA_002275"
     CDS             3446..5026
                     /locus_tag="ACG8OA_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFS4977024.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5224..5447
                     /locus_tag="ACG8OA_002276"
                     /pseudo
     CDS             5224..5447
                     /locus_tag="ACG8OA_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5652..5972)
                     /locus_tag="ACG8OA_002277"
     CDS             complement(5652..5972)
                     /locus_tag="ACG8OA_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFS4977025.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6120..8354)
                     /locus_tag="ACG8OA_002278"
     CDS             complement(6120..8354)
                     /locus_tag="ACG8OA_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4977026.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8833..9099
                     /locus_tag="ACG8OA_002279"
     CDS             8833..9099
                     /locus_tag="ACG8OA_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFS4977027.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9099..10826
                     /gene="ptsP"
                     /locus_tag="ACG8OA_002280"
     CDS             9099..10826
                     /gene="ptsP"
                     /locus_tag="ACG8OA_002280"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFS4977028.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3462 a         1907 c         2208 g         3300 t
ORIGIN      
        1 gcttcttcaa tcaatatttt tcctaaccct tggtgtctgt attcagaaag aatcgccatc
       61 cgctgtaatt tcatttttcc gttttctaaa ggcaaaagac gtacagttcc ttgcggttct
      121 tttttatctg agtacaggac gaagtggata caatgggctt catctttgtc tatttctcga
      181 gacaaaggca caccctgttc gactacaaaa acttgatgcc tgatccgtac agcatctaag
      241 taaatattgc tcattgtatc ttttgtttgt acaactttca tggtctagcc accttccttt
      301 cgtatagtat actggaaaat aaaacaaata aaaagtatgg caagttttca aaaataaaaa
      361 aagaataaaa gaatttttaa cgaatataat atatactagt aagaaagaaa ggaggaaatc
      421 tattgtttga aaaacttaga cagtctaaac taatgttttg gtcgcttgaa ttattgatca
      481 tagctgcatt gatcatgatt tcatcagaaa taaactttat tttcgaaccg atcgggacat
      541 ttttttccac gttatttgca cctgtactga ttgcaggctt tctttactat ttattgaatc
      601 ctattgtcaa gctgttgatg aaagcaaaaa tcaaaagaat atgggctgtc gcaattgtgc
      661 tgctcctttt agttgcagca attatttgga ttttacttag tgtcatccca agtctagtac
      721 agcaaatttc ctcattggca tctaatatgc ctgatttcat caaacaggta gaaagctggt
      781 taaaagaggt agccgaattg cctttgttca aggaagtaga tatcaataaa tattttgagc
      841 aattagacat ttcttacggg acaatcattc agcaattcct tagtggagta tccaatagtt
      901 taggttcgat cgttggaacg atcgcttcgg caaccgtagt aatcattaca gcaccattca
      961 tcttgttcta tatgttgaaa gacggagaaa aactagtgcc aaacatccaa cagtttttcc
     1021 cagaaaaaag aagaaaccaa attgtggagt tattggggca actgaataaa acattggcta
     1081 attacattag cggacaagca atcgaatgtt tattcgttgg taccttcaca tttttaggat
     1141 atatggtaat tggtgtggat tatgctttct tatttggggt cattgcggga ttgacaaacc
     1201 taattccata tttaggacct tatctaggac tagcacctgc atttctagta acagtcttca
     1261 atgatccggt aaaagcatta ctttgttgtg tggttgtgct aatcgtccaa cagttggatg
     1321 ggaatatcat ttatcctaat gtaattggga aatcactgaa aatccatcca ttaacgatca
     1381 ttatcgttct tctagtagct ggaaatattg ccggattgct tggtatcttc ttaggtgttc
     1441 ctttttacgc aatctgcaag acgatcattt cctatataat caaaatcgtc aaagaagaca
     1501 agcaaaatga aaacaagaag aaaattgcta cgacctcgta ggtagtttta gtacacttgt
     1561 ttttattgca aagaaaattg ctttgtgata ccattttaac gtggctatag tgccacgttt
     1621 ttttctatcg taaactaaaa attaatttta taaggagaga atgcgattat gaatgctgac
     1681 cctgagagtc agtcgctgtt agcgcagatt ttattattga tcgttttgac attgatcaat
     1741 gcttttttag cagcttccga aattgctgtt gtttcgatca ataagaatcg aatcgagcaa
     1801 aaagctgatg aaggagatgt gaaatctcaa aagcttttaa aaatattgca gaatccaaat
     1861 aatttcctat cgacaatcca agtaggaatc actttggtca acattttgtc tggtgcttca
     1921 ctagctaata ctttatcgga acggttagct ccggtattag gtggcggcac agcggcaaaa
     1981 aatattgcaa atatcattgt gctggctatt ttgacctatg tatcgattgt ttttggagaa
     2041 ttgtacccta aacgtattgc tttaaataaa tcagaagaag tcgcaaagtt cacttcaggg
     2101 ctgatacgct tgattggtgt agtagctaag cccttcgtct ggctgctttc tgcttccaca
     2161 agtttattgg cgcgtatcac gccgatgact tttgatgatg aagattcaaa aatgaccaga
     2221 gatgaaatgc gttatatgct tgaaaacgaa ggcgtattga ataatgaaga attagaaatg
     2281 ctccaagggg ttttttctct tgatacgaaa gttgcccgag aagtcatggt cccacgaacg
     2341 gatgcattta tgattgatat caacgacact gtggaagaaa atgtgaatga agttttatcc
     2401 gaaaactatt caagaatccc tgtttataac gaagacaaag ataaagtagt cggaatcctt
     2461 catacaaaga atttactaaa agctgcccat aaattcggct ttgataattt agatatcaag
     2521 aaaatcatgc aggagccttt attcgttcct gaaacggtct ttattgatga tctgttatat
     2581 gaaatgaaaa agacgcagaa tcagatggct attttattag atgaatatgg cggcgtcgtt
     2641 gggttagcaa cactagaaga ccttttagaa gaaatcgtcg gagaaatcga tgatgaatca
     2701 gatgaagttg agaatcttta tgaaaaaatc gatgaacacg aatatatcat ccaaggacgt
     2761 atgctgattg atgagttcaa tgaagctttt gacagtgatc tgcatatgag tgatgtagat
     2821 acgatggctg gttatttgat tacagcatta ggcatgattc cggatgaagg cgaaaagtta
     2881 tcgtttgatg tcgataatat tacgctggtt tctgaagaaa tggaaggttc tcgaatcctt
     2941 aaaatccgtg tcatttttca tgatccggaa gaaacagaag cggaacctga agaagaacga
     3001 agatacttca gaaaagaatt cgaagatgac gagccaagaa gataattgga aacttggtta
     3061 tgaaagacgt gttgcttgaa taaacaagca acacgtcttt tcttttagtc agctgacaaa
     3121 aaaatatttg actcttatgg tttggtggct ttttcattcg ctgaatccat gctatactga
     3181 ttcagttgta gtagttgaca aaggttgtga aaaagctatc ctgcattaag taataagagc
     3241 agccaagcaa aaacagcttt tcgtattttt ggcttgggtg ggcttattgc tgataggctg
     3301 tctctagaac gccgtttact cggaaaaaga gactgctaca cggcttttgt cacagcccta
     3361 tatgaaattg tctttcttta agagatcgga caatctgtgc ctagagaaaa tataaataat
     3421 tgaatggaag aaatggagtt aggttatgaa taatccaaaa ttaaagcaac aagtggacaa
     3481 tcgcagaact tttgcgatca tttcccatcc ggatgctggg aaaacaacga ttacagaaca
     3541 attactattg tttggtgggg ctatccgtca agccgggacc gtaaaaggca aaaaaacagg
     3601 taattttgcc aaatcagact ggatggaaat cgagaaacaa cgtgggattt ctgttactag
     3661 ttctgttatg cagtttgatt atcaagggaa acgagtaaat atcttggata caccaggaca
     3721 tgaagatttt tctgaagata cttatcgtac tttgatggcc gtggatagtg cggtcatggt
     3781 aatcgatagt gcaaaaggga tcgaagcaca gacaaagaaa ttattccaag ttgtaaaaaa
     3841 acgtggcatc ccgatattta cttttatcaa caaactagat cgtgatggtc gtgaaccatt
     3901 ggaattgtta gaagaattag aagagctatt agatatcgaa tcttatccga tgaattggcc
     3961 aatcggtatg ggtaaagggc tagaaggtct ttatgatatc taccataatc gtgtagagtt
     4021 ttatcgtccg gaaaattatc aggatgaacg attagtagaa ttggatgaag atggactgct
     4081 tcccgaaaat catcctctaa ctgaaaattc cttatatgaa caagtcttag aagaagtaga
     4141 actaatcaaa gaggctggag atgcatttaa tcaagaaaaa attgctcgtg gcgatcagac
     4201 accagtattt tttggttctg cattgactaa cttcggtgta cagacttttt tagagacttt
     4261 cgttgaattg gcaccagctc cttatggaca caaaacacaa gatgatcaga tggtaagtcc
     4321 ttatgaggaa gaattttcag gtttcgtatt caagatccaa gcaaatatga atccagccca
     4381 tcgtgaccga atcgcttttg tccgaatctg ttctggaaca ttcgaacgag gaatggatgt
     4441 ctggttggaa cgtacaaata aaaaactgaa attaagcaat gtcacacaat ttatggcaga
     4501 ttcgagagaa aacgtcgaaa aagcagtagc aggcgatatc atcggtgtat atgatacagg
     4561 caattatcag attggggata cattgttcga aggaaaacta aaagttgctt atgaagaact
     4621 cccttcattt actccagagc tgtttatgaa agtgactgca aaaaatgtca tgaaacaaaa
     4681 atccttccac aaaggaatct atcaattagt tcaagaagga gcgattcagc tttataaaac
     4741 atatctaacg gaagaatata tcattggagc tgttggtcag ctacaattcg aagtattcca
     4801 atatcgtatg ttaaatgagt ataacgccga agtaatcatg tctccaatgg gacacaaaat
     4861 cgctcgctgg atcgatccag aagatttgga tgagaagatg agttcaagtc gcaatatctt
     4921 ggctcgtgac cgtttcgacc aaccgttgtt cttattcgag aaccaatttg cagaacgctg
     4981 gtttgcggat aaatatccca atgtaaaatt aaaaagtttg atgtaaagaa gtcttgacac
     5041 aagctgccct gcatcaagta ataagaatag ccaaacaaaa acagcctctc atattttgtt
     5101 tagagccgtg acggattttc cgtgacggct ctttttacta tattaaaatc atagaaatgt
     5161 ctaatcaaaa aaatataaaa attattttgc gtacatatga ttaaagaaag gagagtgtga
     5221 cacatggaaa cagaatggca aagacgtgaa agaaaaaaac agaaaaagaa gagatgttac
     5281 gattatgtcg attattagat gtaaaagact gggacaaaaa cgagcaaatt gcacaaaaag
     5341 tacgatcaat catccataaa gaggaaaaag ttaaaaataa agacacgcgt agaactgaaa
     5401 atgaaaagag aaaagtacaa aagaaaagag aaaaatcaaa aagctagaaa gaaaaagagg
     5461 ctgagacaga agcgtttaac tccaagaaat aagctggaat tcacaaaaat ttgaaaagca
     5521 attttcgtga atttcagctt atttccgaag gagttgcttc tgctttcgcc gtttatacgt
     5581 gtttagagcg tgagacaaaa gtgttcttta cttttgtccc acgctcttta attattatgt
     5641 tcaaaatttg tttacacggc taatgtagcg cctaattcat gttttggaat cgagatttca
     5701 acaaggcctg ccaattcgat tactttgatt tcatcctcgt ggtaaacggc gtctttgatt
     5761 ttatctaaga aataattttt agcatcttcg atttctttcg gctggatttt acgtttgtca
     5821 ttgatcagca gaatttcatt atgttcaggg gcttcagtgt agacaatagt ggtatttccg
     5881 gctgtgtaga ccttgactag atgggcatca gtgttttcaa gctggtttaa tacaagacgg
     5941 gaatggctgt ttgttacatt taccaatttc atggcgctca cctcttttat gtatttttaa
     6001 ttgcttgttt ctcttattat aatttctttt ttataaaatg aacagtattt tgacttttct
     6061 gaaaaaagaa tgagacggca tttctcaaag aaataccgtc tcacgctata aaactggatt
     6121 tattcagccg tttcttcggc tgattcttta gccaaacgtt ctggtttaga agtaacgatg
     6181 atttttccat ctttatccag ttttgctttc aactcatgga tagaagggtg atcaagataa
     6241 aattcagcga taccatcttc gatttgttct tggatcgtgc gtcgaagagg acgagcaccc
     6301 atagatggat catagcctaa gtcaactaat ttttctttga cattcgtagg aacttcgatg
     6361 tgaagttgct gtgctgccag catttggttg acatcgtcta gcattagtgt gacgattgtc
     6421 atcaagtttt cttttgataa tgcactgaat tcgatgatcc cgtcaaaacg gttcagaaat
     6481 tctggtgtaa agtagttgtt taattggttc aatacagaac gagtaacacc ttcgcgtgcc
     6541 gcaccaaaac caacgtttgc ttctacttta ccagttccgg cattacttgt cataatgatg
     6601 atcgtatctt taaagctgac cgttcttcct tgtgcatctg tcaatcgacc atcatctaag
     6661 atttgcaaga acatatgcaa aacatctgga tgagcttttt ctacttcatc taacaagaca
     6721 agactgtaag gatttctccg tactttttct gttaattgac cagcttcttc gtatccgaca
     6781 taacctggag gagaaccaat caatttagag acactatgtt tttccatata ttcagacata
     6841 tcgaaacgaa tcattgaatc ttgtgaaccg aatagttcgt aggcaagttg tttcgcaagc
     6901 tctgttttac ctacaccagt tggtccaaca aataagaagg agccgattgg acgattctgt
     6961 ttgtttaatc caacacggtt tcggcgaatc gctttcgcta ctcgatctac agcgttatct
     7021 tgtccgatga catgagcttt caagtcatct gctaagtttt tcagttgtgt ctgttctttt
     7081 tcttttaatt cacccactgg aatacctgtc cgttgttcaa cgattttttc catatctttt
     7141 tcggtaatca caggtgtttc ctcttcagtg agctgacgtt ctttcatttt ttgaagttta
     7201 ttgatctgat cgcgataata agctgctttt tcgaaatctt cttctcgaga agctaaaact
     7261 ttttgttctt ctgcttcttg cagttttttc tcgattgttt taggatcaac gatttggatc
     7321 gtcaagtttt tcttagaacc tgtttcatct agcaaatcga tggctttatc cggcaagaag
     7381 cgatcttgga cgtaacgatt ggataaagtt gcggctgctt cgatggcctc atcagtatat
     7441 tttacatgat gatagtcttc ataacgtttt tgcaaacctt tcaaaatact gatcgtttct
     7501 tcgacagttg gttcatctac ccgtacaggt tgcatacgac gttctaaagc agcgtctttt
     7561 tcgatgatgc ggtactcatt taaggtagta gcaccaacca tttgcaattc tcctcgagct
     7621 aaagctggtt ttaaaatatt accagcatcc atatttccat caccagcaga accagcaccg
     7681 acgatttcgt gaacttcatc tatgaatagg atcacatttt ctgcttgacg gatctcttca
     7741 atcaattttt gcatacgttc ttcaaattgt cctcgtatac cagtaccttg gactaaagat
     7801 acgacatcta aacgaatgac ttctttgtcc attaattttt gcggtacatc accatctacg
     7861 attttttgtg ctaaaccttc aacaactgct gttttaccaa cacctggttc tccaatcaag
     7921 acaggattgt tcttggttcg gcgatttaaa atttcaatga ctcgtttgat ttcgtcatcg
     7981 cgtccgatca caggatcgat atcaccttgt cgtgcttgtt ctgtgatatt gattccgtat
     8041 tctcccaata aaccttctgt attgccttga ggtggctgac caccattgaa gttgttgttt
     8101 ccgccaaatt gtgttggtgg tgtttgctca taatttcctt gctgttgcat ttggcgagac
     8161 atggaacgga aaagatcgtc caaactgcca aatccaaatg gatcattttg ggccatatta
     8221 gtcagacctc cattttgttg atttttgatt ttttgataac agctctgaca gtaatcgagt
     8281 tgtgtccgct gaccattgac agtggcatac aagtgaatcg ttgcctcatt cttgccgcag
     8341 ttttgacaaa gcatgaatca ttcacgtcct ttcacaaaaa tgggtacatc catattttac
     8401 atctcgtttt tcatgaggta aaagaataac ccctagtttt ttaagagatt tatgaggtag
     8461 aaaaggtgaa atctctattg accattcctg acctttgact cttattatac taaaaataaa
     8521 aagggaagca agtaattcgt ttcatatgaa aacaaagagg gtcaaaggaa agaatgataa
     8581 acgtcaggag aagagaaagc gacaagaaaa aggaaataaa cacgtttgtt ataaaatgtg
     8641 aagaaaagga tgaaaaagaa tccggaataa tttgaatact tttatctgaa attgaatgaa
     8701 aactgttgta tctaacgaaa aaaaatgtta atctttacag atgaaagggt ggcatttgtt
     8761 aggattctct gaattactaa aaaagaaaac cctcacataa aaacaacact cacttaaagg
     8821 agaccgatta atatggaaaa gaaagaattt cacgtagtag cagaaactgg gatccacgct
     8881 cgtccagcta cactattagt acaaacagca agcaaattta actctgatgt aaacttagag
     8941 tataaaggta aatcagtaaa cttgaaatca atcatgggcg ttatgtcttt aggtgtaggt
     9001 caaggttctg atgttactat tactgcagaa ggtgctgacg aagctgatgc aatggcagca
     9061 atcgttgaaa caatgaagaa agaaggctta tctgaataat ggttgaaatg ctaaaaggga
     9121 ttgccgctag tgacggtgta gccgttgcaa aagcttacct gctagttcaa ccggatttaa
     9181 cattcagtaa ggcaacagta gaagatactg cggctgaaga agctcgtttg gatgctgcat
     9241 tagctaaatc tactgaagaa ttgcagcaaa ttcgtgaaaa agcagcgcaa agcttaggtg
     9301 aagcagaagc gcaagtattt gatgcccatt tgatggttct ttcagatcca gaaatggtag
     9361 gtcaaatcaa gcaaaatatt aaagacaaca gcgtaaatgc tgaatcagcg ctaaaagaag
     9421 taacggatat gtatatcggt atgttcgaag caatggaaga taatgcttat atgcaagagc
     9481 gtgctgcaga tattcgtgac gttgctaaac gtattttagc gcacttatta ggcgttactt
     9541 taccaaatcc atcaatgatc aacgaagaag tggtcgttgt tgcacatgac ttgactccaa
     9601 gtgatacagc acaattagat cgcaattttg taaaagcgtt tgtaacagac attggtggac
     9661 gtacttcaca ttctgctatc atggcacgtt ctttagaaat tccagcaatc gttgggacaa
     9721 aagaaattac tgctaaagtc aaagaaggcg atattttggc tgtcaacggg atcgaaggcg
     9781 atgtaatcat cgatcctaca gatgaacaaa aagctgaatt tgaaaaagca ggtgcagatt
     9841 acgcagcaca aaaagcagaa tgggaaaaat taaaaaatgc agaaactgtc acagctgacg
     9901 gcaagcattt tgaattagct gcaaacatcg gaacaccaaa agatctagtt ggtgtccata
     9961 ataatggtgg agaagcagtt ggtctgtacc gtactgaatt cctgtatatg gattctccag
    10021 acttcccaac agaagatgac caatatgaag cttacaaagc tgtattagaa ggtatggaag
    10081 gaaaacctgt agttgttcgt acaatggaca tcggtggaga taaagaacta ccatatctgc
    10141 aattacctca tgaaatgaat ccattcttag gatatcgtgc attgcgtatc agtttatctg
    10201 aacaaggcga cgaaatgttc cgtacacaaa tgcgtgcatt gcttcgtgca tctgttcatg
    10261 gtaatttacg tatcatgttc cctatggtag ctacactcaa agaattccgc gcagcaaaag
    10321 cgatctttga agaagaaaaa caaaaattga taagtgaagg caaggaagta tctgatacga
    10381 tccaagtcgg catcatgatc gagattccag cagcagcagt cttagcagac aagtttgcaa
    10441 aagaagtaga tttcttctct gtaggtacga acgatttgat tcaatacaca atggcagcag
    10501 accgtatgaa cgaacgcgtt tcttacttgt accaaccata taacccatca attttacgtt
    10561 tgatcaaaaa tgtaattgat gcagcacacg ctgaaggaaa atgggcaggt atgtgtggag
    10621 aaatggctgg agatcaaaca gctgttccat tgctagtagg tatgggtcta gatgagttct
    10681 caatgagtgc gacatctatt cttaaaacgc gtagtttaat gaaacgcttg gacacagcga
    10741 aaatggctga acttgctgat cgtgcattga aagaatgcga tatgatggaa gaagtcgttg
    10801 aacttgttca tgaatatgta aaataaacaa cgtatcaaaa gctggtgtgg cttgtaaaag
    10861 agctacgcca gtttttt
//
LOCUS       DAZULO010000088        10743 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000088 DAZULO010000000
VERSION     DAZULO010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.090"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACG8OA_002281"
     CDS             33..656
                     /locus_tag="ACG8OA_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977029.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACG8OA_002282"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACG8OA_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4977030.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACG8OA_002283"
     CDS             2152..3033
                     /locus_tag="ACG8OA_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4977031.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACG8OA_002284"
     CDS             3036..3806
                     /locus_tag="ACG8OA_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4977032.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACG8OA_002285"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACG8OA_002285"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFS4977033.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACG8OA_002286"
     CDS             5117..6280
                     /locus_tag="ACG8OA_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS4977034.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACG8OA_002287"
     CDS             6397..6807
                     /locus_tag="ACG8OA_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4977035.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACG8OA_002288"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACG8OA_002288"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS4977036.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ACG8OA_002289"
     CDS             9380..9664
                     /locus_tag="ACG8OA_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977037.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="ACG8OA_002290"
     CDS             9824..10060
                     /locus_tag="ACG8OA_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4977038.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="ACG8OA_002291"
     CDS             10057..10455
                     /locus_tag="ACG8OA_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS4977039.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3756 a         1784 c         2263 g         2940 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg
    10621 aaatagtggt ttagaactag tgggctgatt ttttatatat tttagtttac ataatgtgta
    10681 ttatacaaag tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc
    10741 gtc
//
LOCUS       DAZULO010000089        10726 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000089 DAZULO010000000
VERSION     DAZULO010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.091"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..1091)
                     /locus_tag="ACG8OA_002292"
     CDS             complement(243..1091)
                     /locus_tag="ACG8OA_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4977040.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1255..2157
                     /gene="gnd"
                     /locus_tag="ACG8OA_002293"
     CDS             1255..2157
                     /gene="gnd"
                     /locus_tag="ACG8OA_002293"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFS4977041.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2159..3160
                     /locus_tag="ACG8OA_002294"
     CDS             2159..3160
                     /locus_tag="ACG8OA_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFS4977042.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3157..3441
                     /locus_tag="ACG8OA_002295"
     CDS             3157..3441
                     /locus_tag="ACG8OA_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFS4977043.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3474..4271
                     /locus_tag="ACG8OA_002296"
     CDS             3474..4271
                     /locus_tag="ACG8OA_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4977044.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4285..5118
                     /locus_tag="ACG8OA_002297"
     CDS             4285..5118
                     /locus_tag="ACG8OA_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4977045.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGGFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5136..5993
                     /locus_tag="ACG8OA_002298"
     CDS             5136..5993
                     /locus_tag="ACG8OA_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4977046.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6015..6524
                     /locus_tag="ACG8OA_002299"
     CDS             6015..6524
                     /locus_tag="ACG8OA_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS4977047.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6561..6962
                     /locus_tag="ACG8OA_002300"
     CDS             6561..6962
                     /locus_tag="ACG8OA_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977048.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7074..7427
                     /locus_tag="ACG8OA_002301"
     CDS             7074..7427
                     /locus_tag="ACG8OA_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977049.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7461..7904
                     /locus_tag="ACG8OA_002302"
     CDS             7461..7904
                     /locus_tag="ACG8OA_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4977050.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7921..9006
                     /gene="uxuA"
                     /locus_tag="ACG8OA_002303"
     CDS             7921..9006
                     /gene="uxuA"
                     /locus_tag="ACG8OA_002303"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS4977051.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8999..9712
                     /locus_tag="ACG8OA_002304"
     CDS             8999..9712
                     /locus_tag="ACG8OA_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4977052.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9919..10068)
                     /locus_tag="ACG8OA_002305"
     CDS             complement(9919..10068)
                     /locus_tag="ACG8OA_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977053.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10464..10664
                     /locus_tag="ACG8OA_002306"
     CDS             10464..10664
                     /locus_tag="ACG8OA_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4977054.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3589 a         1696 c         2320 g         3121 t
ORIGIN      
        1 acaaaaataa taaaaaaaag aaaagataaa ttttttacta ctgtatacta actttgttat
       61 cgcatctaac aagtaactcg aataatacca atacaacctt ctccaaagat gcgatgacat
      121 acgttctaac ccaccgacta ttgatgttct gtaccaccct tgcagaacca aaaaagccac
      181 taccaaatat ttggtagtgg ctttttttgt tttatggatt tttcgctttt tctgttatgg
      241 tgctattctt tgatttcaga aatagtatgt cgaactttag cgatagaacg acgtgattct
      301 ttttcgttgt aaaacatcaa aatcacatat agagagtcca aaatgcttag ttgagaaata
      361 cgggaagcta aagcttcaga tcggaattct gtttcttcag aaatagaaat aaaaacgaca
      421 tcgccaagtt tagcaaggga agaattagct tggctagtaa taacgattgt ttttgctcca
      481 gtttttctta cggtttcagc aatatggatt gtttcttttg attgccctgt gtgagaaatc
      541 aaaacagcac agtctttttc cgtcaataaa gaagcttcca tcaattgaat atggtaatcc
      601 gtgctgtgag aagttgcgat aggggaacgc aaaaatttat gataagcgtc agttgctaca
      661 atctctgaac cgccaacacc gaaaaaatag acacgtttag attcattgat aatagtagcc
      721 gcttttttta gatccttttg ttgtaacaac ttttttgtgt catttaaagt tgaaatacta
      781 gactcgaaca ccttttgcgc catagtgagc tcagtatctg ttttctgaat attttcgtgg
      841 atagaaatag cagatagatc attttcttgg atcaacatgg ccattttaaa atctttaaat
      901 ccattataac caagtttttt tgtaaattga aaaaaagtag agtcagcaat gcccagttca
      961 cttgctaaat tgctaatagg actatgactt actttgcttg gattttctaa aatataatcg
     1021 gcgatagcct gttcttttgg gctgaagtcg ttgtacattg ttttgatttg tagtcttatg
     1081 gagacgctca tgagaattcc tcattttcat aataagatta tcattttttc cgttctcatt
     1141 aatgttaaag atacaaggag agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct
     1201 atttacaaaa aaattttttt gtataatgag ggtataagaa atggaggcgc tatcatggaa
     1261 attggtgtaa ttggtttggg aaaaatggga ttgaatttag ctttaaactt aaaagagcac
     1321 catcacaaag tacaaggact cgacatttca agtacagctg ttagtcaggc aagagaaaat
     1381 gacattcctg cttatcaaga gatctctgaa tttctagcta cttttgagca gaaaaaaatc
     1441 atctggttaa tgcttcctgc tggagagata accgaaaaca tgttaagaaa actttttcca
     1501 aaactcggac caggagacat tattattgaa ggcggaaact cttattataa agattcaatc
     1561 cgtcgagcag aagagtttca gaaaaatgaa atcggttact tggattgtgg gacttctgga
     1621 ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg gaggagataa agaaactttt
     1681 ctatccgtgg aacaattatt caaagatgta gcaattgaaa gaggatatct gtatgctggt
     1741 gcatccggaa gcggacactt cttgaaaatg atccataatg gaatcgaata tggaatgatg
     1801 caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt tgacttggaa
     1861 caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg gctggctgat ggaaatcatg
     1921 gaagatcagt taaacgaaca tcccaattta agtaattatc ggggaatcgt ttctgcatca
     1981 ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt
     2041 gcaactagtt tgttcatgag gaatctttca caggaagaag atagtttttc agcaaaagta
     2101 gtttcagcac taagaaatgg gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat
     2161 gccagattat catatagcaa tcgttaattc cagcagtttc ggaagagtct ttccacacca
     2221 cttggaaaga ttaaagtcag ttggagacgt tcatttcttt actgttgatc aagagattcc
     2281 aggaaaagaa ctagcggagt tacttaaagg attcaatatc attattgcaa gtgttacgcc
     2341 attttttcga cgagattttt ttgaaaacaa agatgaactg ctattgattt cccgacatgg
     2401 tattggttac aacaatattg atttaaaagc agcaaaagaa caccagacaa tcgtatctat
     2461 catacctgct ttagttgaac gaaatgctgt agcagaaaat aatataacta atttgttgtc
     2521 catgcttcga cgcacgacac aagcacaagc aagagtcaag gacgatcgat gggaagatcg
     2581 ggcggagttc gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa
     2641 taccggaagc ggagtaatcg aaattttgcg taacggtttt cgctgtgatg ttctagctta
     2701 tgatccttac aaatcagatt tatacatcga aagctttggt gcaaaaaaag tagaacttca
     2761 agaattactg gaacaatcag atatcatttg tttatgtgcg aatttgacag aagagaacta
     2821 tcacatgatt tctgataaag aagtcgatca gatgaaagaa aatgtttatc tatctaatag
     2881 tgcaagaggc gctctagttg atgaagaagc gatgattaat ggtctgaaaa gcggaaagat
     2941 cgcaggatat gccacagatg ttttagaaga agaaccggga cgtcaagatc atccgtattt
     3001 agcgtttgaa caagtagtga ttacgccaca tatatcggcc tacacaaggg aatgtttaga
     3061 agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc ttcttcctga
     3121 acgttctgtc cagacaaaga gcaactatat aaaaggatga tgaagaaaat ggtgaacggt
     3181 ttaaaagtga aaacaggccc tcaattttat ttatatgaag aaggtggtat ttcaaaagtt
     3241 tctgatctct tgaaatcata cggcgcgaaa agagtactgg tcacccacgg aacggtatca
     3301 tgggaaaagg cgctacctaa attagtattt ttgaatgacg aaacgattca atttttttat
     3361 catcgttatt ctggagaatg tagttatgct gaagccagac gaatagctac aatcatcaaa
     3421 aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa actgaccgat ctagtgggtt
     3481 atagtgccaa tcttgcaaga atcgatttcg gagtcatccc tactttagct agtaattgtg
     3541 ctccatggac acctcttgct gtcatgtata aggagaatgg acaagcagaa gggaaaacag
     3601 aacactttta cagacaagct gcttttttta taacagatcc ccaattagtg attgattccc
     3661 ctgttccata tttcattgcc gggttagcag atacactagc aaaatggtat gaatctgagc
     3721 tcatcttaga acaagatttt ttgcaagatg aatcattttt gaagctagca caagatactg
     3781 cgaagatctg caaagaagag attttgaatc attctgctaa agcaatccaa gatatggcac
     3841 aaagaaaact gacatcggag ttcaaacagc tttcagagat tatctttgct gttgctggtt
     3901 tagttggagg attaggagat aaatacgcaa gaaatgcggc agcacatgct atgcatgacg
     3961 ctatcagtaa atatattccg gaaagccatc gttttctaca tggagaaaaa gtagcgtatg
     4021 ggattttcta tcaactggca ctagaaggtc gctgggaaaa aatagacgag ttatatcctt
     4081 tgtatcggga attagaactg ccggtttctc tagaacaaat gggattattt ccgaaagata
     4141 aacagataat agataaaatg gctgaattta ttgattcgaa agagaaagtg catttgattc
     4201 ctatagaggt aacacagaaa tcactgatac agtcaatgat ggaactggaa ggttatatca
     4261 gaaagatctg aaggaggagg acacatggaa acattaagtg tagtacaaag tttgttgatt
     4321 gctttatggg tagccgcggt tatgtcccgc tggttaggtg gaggagccac gttgacctta
     4381 cgtttttctc ctttgatgac tggattagtc gtcgggttga tcatggggga agttgcacaa
     4441 gcgatgattg ttacagccgc tttgcaaatg atttatatgg ggggtttttc tccaggagga
     4501 tcaatgccag ctgaaccatc tattgcagca gctatcgcag taccagtcgc attattaggg
     4561 gatctgtcgc cagaagcagc gattgccgtt gcggtgccag tcggattgct cggaagttac
     4621 ttgtatcagt ttcgtttttt catcaatact tttttaggta aatatacaga caaagctgta
     4681 gaagatttga atacaaaagc tatggcccga tcaattattc tttatccaac ggttgcctca
     4741 tttttattat tcgttccgct tgttttcatt gctttatatt taggggcgcc attgatcgct
     4801 gatgtaatca aagctttaga gggaacagtc gttatccaca ttcttgaagt agtaggtgga
     4861 gggttagcgg caatcggtat cgcgacaact gtatacgtta tcggtcgaaa agattatctg
     4921 gtctttttct tccttgctta ttttatgagt gttgttttga aatctttaga tattacgatg
     4981 gttacttatg ccatttttgg gattattatc gctttgatct ttgtccagtc gcaaaaaggg
     5041 aaacaagtac agcctgccac aagcaataac aattcagcat cttttgatga cgaagatgac
     5101 tatgatgacg gattttaatt taggaggaat gggaaatggc tgaaataatg gataatcaaa
     5161 caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg agatggcatt
     5221 ttgcaaacga aattcctcac tcttttgaac gttacttggc gccatcacta ctatacggaa
     5281 tgatgccaat cttgaaaaaa ctttataaag atgaagactc tttaaaagca gcatataaac
     5341 ggcagttact gttcttcaac actcaattaa gctggggtgg cggtgtgatt actggcctaa
     5401 tgtcttctat ggaacagcaa agagcagaag aagagtatga agaaaaagag atcttgatgc
     5461 aggatgactt gatgtataac acgaaagcgg gattgatggg ggcactagca ggaatcggag
     5521 atgcaatcga ttcaggaacc gtccaatata tttttatcgc aatcgcggta ccttgggcac
     5581 aacagggaag tcctttaggc gcactttttc cttttatctg ctttgggttg tatcaggttt
     5641 tactaggggt atttttcgca agacaagctt tcactatggg ccgtaatgct acaggcctaa
     5701 tgcaaaacac tggtgttcag acagcgattg aaatgctatc agttttaggg ttgtttatga
     5761 tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc attgaaattt gcgatatccg
     5821 gacgtgagtt tgttatccag gaaattcttg atcaaatcgt gccagggata ttacctttag
     5881 ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc acacaagcat
     5941 tattatggct gactggaata ttaattgtgc tagcaggaat cggtatttta taaaaagaag
     6001 ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg
     6061 ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg
     6121 aagttgtagc aaaagataaa tttatgagag atctttacaa aaatgcagga agccgtacag
     6181 gacaaaaaac aattgtcatt acaccagaga aagctaaatt ctattgggat gaatatcaat
     6241 ttaaagaata caattgtatt ttgatcgcaa aaacagtcca aacgatttac gacttagtaa
     6301 aacacggaat ccccatggaa gaattgaata ttggagggat tgctcaaaaa aatcctgata
     6361 cagatctttt agtgacgaaa tctgtttatt taaataaatc agatgctgaa aaattgaaag
     6421 aaatgaatga acaatatggc gtaaagaata tttattttca agccactcca tctgcaccta
     6481 aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa
     6541 aagataaaga gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt
     6601 atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat
     6661 tagtatcaga tatttacagt atgagaacag atgtgacagt cattgcttac gatacgctag
     6721 cctaccgatc aaaaaatatt tatgttggag atatcgaaaa agcagagaaa gttatctgta
     6781 cttattacga tacacccgtt catgcattag gaagttattt catgtttgat tggaaagacc
     6841 aaaggaaaaa aacaatctac tctatattat tgtccttcat tttgttgttc agtttaggtt
     6901 ggtggggaat gatgatatat aacaaaaatc ctcatcacgt attcgatctg ctatccgtat
     6961 aaacaggtat cactgtacta gcatttggga gctacttttt cttattggga aaagcagctc
     7021 gcggatggcc tagcagacaa acttttattc gaaatacttc atccatatta acgatgctag
     7081 aaatgatccg tacgattgat gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt
     7141 atggagaaaa gggcttagac agcgtgagat cgggtatgaa aaaagaggct attctttttt
     7201 atctagactc tgttggggca gatacaccgc tacagttttc gggaaattat ttttcaaata
     7261 aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat tatattttta
     7321 gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa aacagattta cgagggaaaa
     7381 catttaattg gcaaaatgcc aatcagatta ttgctctttt tcgataagta aaaagtgcaa
     7441 aacaaggagt gagaagacta atgttggaaa tcatgatcgt tacacatggt aagttgagcg
     7501 aaggactgaa agatgcggcg gaagtaattg tcgggacatc gagccatatt gaaacaatgt
     7561 gcttagagtc tggggaggac atccaaacat tcggagagaa ggtcaaagaa aaattaacta
     7621 tgatcaacac agcagaaccc atgctagtat ttgttgatct aattagcgca agtccgtaca
     7681 atcaagcgct cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc acttatgtca
     7741 tagggggcgt gaatctgcca atgttgttag aagcaatcaa tcatcagatg cttcacacac
     7801 cgatagaaga agcggtggaa caaattgtca aacaaggaaa agataattta actgtttggc
     7861 atgcttcagc ggaagaaaaa aaattagatg aggacgattt ttagaaggaa ggctgaaaag
     7921 atgaagtggg gatttagatg gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt
     7981 cgccaaatac cgggaataaa tgggataatt ggaactttgc tgactaaaat gcctggagat
     8041 gtttgggaga tttcagaaat tgaggagttg aaagcatctg ttgagagcca aggactggaa
     8101 ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca aggcagggac agatgaacga
     8161 gattactata ttgataacta tatacgaaca atcaaaaact tgtctcgatg tggggttcga
     8221 ttgatttgtt atagcttcaa accaattttt ggatgggcga aaactgattt gtttaatgaa
     8281 aacaacgatg gaagctattc tcttttatac gatcagaaaa tcgtcgatca gatggagcca
     8341 ggagaaatgt atcaattgat acacagccag tcaaagggat tcaaattgcc gggttgggaa
     8401 gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa
     8461 gaattatttg aaaatctgac ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca
     8521 gatgtaaaaa tggctatcca tccagatgat ccgccatggg aactttttgg ttttcctaga
     8581 gtgacgaaaa atcttggcga cttgaagaaa attctaaatg cagtagattc tccatataat
     8641 ggactaacgc tgtgcacagg atctttaggg gcaaatccgg aaaacgattt agttgaaatc
     8701 atccatgagg taggttcgcg catccatttt gtccacttca gaaacgtgga gtttttagga
     8761 gaaagaaagt tcaaagaaac ggcacatccc tctacagaag gctcccttga tatgtatcat
     8821 attatgaaag cgttggtgca agaagggttc gacggtccta tacgccctga tcatggtaga
     8881 accgtgtggg gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc aatgggactc
     8941 acttacatgc aaggtctata tgaggcgata agtaaggaaa aaccactggg agagcattat
     9001 gaataaaatc aagctcttca tctttgatat ggatggcctt ttattagaaa ctggacgttt
     9061 agcttatcgt gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt
     9121 ctattattta ctaactggcc aaacagaaat ggcgataaga gaacaaatgg gcattttgta
     9181 cggagaagat gttccatata tcaaatggcg agaagcaatc aatcaatata aagaaaaaat
     9241 cgtgaaagaa gataagagag tatatacgaa aaaaggagca gaagaaattt tatcttttgc
     9301 aaaagaaaga ggaatccata caatcgtggc ttcgtctaat acacgggtaa aagtagaaat
     9361 gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa atcatttcag gggatgatgt
     9421 aaaaaaagga aagccggaac cagagatctt tttgaaagct tgttcaaaaa tgaatattcc
     9481 tccaagtgaa gcacttgtat ttgaagactc gattgcaggg atcgaagcag caaggcgagc
     9541 gggcattttg tcttttctca tcaaagatca tttgataggg ttgcccgatc ataatgggaa
     9601 gcataagttg aaagtagatg taagtttata tgaatccaat aaagcagact atgaatttta
     9661 tgacctgcat caagcaaaac gatttttaga aagtaaaggg ttgtatctct aataaagagg
     9721 actattttct agtcctcttt atttattgtc ctgcacgact gagggaacag atgaaaaaga
     9781 agtaattttt tattttgaag tatctacctt atttgtagtg aacaatttag aacaaacaca
     9841 aaacgcatct actgttatct accagtagat gcgtttaaag agaagaacga gaatcgtcca
     9901 tatgagggtt tccactcctt atgcagattt ttttaattct tgcactgctt gttcgatcgt
     9961 aacacctgtc gctacatgat tggtattttc tttgtcaaaa acgacaaata gattcaaatc
    10021 tgtatccaag gttactcgat attgtatatc tttgttatag aatgtcatag ccattctcct
    10081 ttctttaaat acttgctgaa aggcaacgaa cgcgttgata acgaaattcc atttataagt
    10141 ataccaccaa ttgaaaggtt ttcatagtta agaaatttta aagtcaaaag ttgcagataa
    10201 aagcaaataa acaagaaaac aaaaagtgaa tcaatcgcgg ttattcaact ttttcttcga
    10261 ttcgaagaca atagaagaca atgaatttta tatttcattg cattatcctt gtgttattag
    10321 aaaaagcatg atatattaaa tgagtaatct attttgaaac gtaatttgag cgatatatct
    10381 acatagataa aactcctttt gcctatcaaa tatttattgc aacgaaaaaa cgtacattga
    10441 tacgtatcag gaggaattta tttatgaata acggtacagt aaaatggttc aacgcagaca
    10501 aaggttttgg ctttatcact ggtgaagatg gaaatgatgt atttgcacat ttttcagcta
    10561 tccaaggtga cggcttcaaa actttagaag aaggccaagc agtgtcttac gatgttgaag
    10621 atggtcaacg tggccctcaa gcaacaaaca ttgttaaagc ttaataaaaa ttgactttta
    10681 accgtctttt cgaaagaaaa gacggttttt ttgtacgaaa aaattc
//
LOCUS       DAZULO010000090        10614 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000090 DAZULO010000000
VERSION     DAZULO010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.092"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            315..1094
                     /locus_tag="ACG8OA_002307"
     CDS             315..1094
                     /locus_tag="ACG8OA_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977055.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            1140..2084
                     /locus_tag="ACG8OA_002308"
     CDS             1140..2084
                     /locus_tag="ACG8OA_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS4977056.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            2100..2879
                     /locus_tag="ACG8OA_002309"
     CDS             2100..2879
                     /locus_tag="ACG8OA_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFS4977057.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            2891..3589
                     /locus_tag="ACG8OA_002310"
     CDS             2891..3589
                     /locus_tag="ACG8OA_002310"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFS4977058.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3617..4381
                     /locus_tag="ACG8OA_002311"
     CDS             3617..4381
                     /locus_tag="ACG8OA_002311"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFS4977059.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4425..5876
                     /locus_tag="ACG8OA_002312"
     CDS             4425..5876
                     /locus_tag="ACG8OA_002312"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS4977060.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            5908..7044
                     /locus_tag="ACG8OA_002313"
     CDS             5908..7044
                     /locus_tag="ACG8OA_002313"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS4977061.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            7074..8150
                     /locus_tag="ACG8OA_002314"
     CDS             7074..8150
                     /locus_tag="ACG8OA_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFS4977062.1"
                     /translation="MHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKSDYDEEIISLG
                     GKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKAAKKAGIPIRI
                     AHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKFTLIPNAVDAK
                     KFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFHAIKNNSVLLI
                     IGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLFEGVPLVGIEA
                     QFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRDSERSILIKAD
                     YNIQTAYKILESKYYELFELIQRG"
     gene            8156..9178
                     /locus_tag="ACG8OA_002315"
     CDS             8156..9178
                     /locus_tag="ACG8OA_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HFS4977063.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            9200..10255
                     /locus_tag="ACG8OA_002316"
     CDS             9200..10255
                     /locus_tag="ACG8OA_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4977064.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
BASE COUNT         3892 a         1473 c         2159 g         3090 t
ORIGIN      
        1 taactattat taattcaatg ttaatttttt tgttattcta ttttttgtgt aacttttaaa
       61 aacacctatg aaaattatgg aaataaaaat gaaatcgatt aatttttatc ttattaatgt
      121 tttatttact tttaaatcgt aaattgccct agtcaagaaa acagtttgtg gtatgattat
      181 atagggaaaa cttgtttaca aatatttctg tgaatatatt agtttaatta tgttttttta
      241 aataaaaagc tgtcattttt atgtaagtga attatatctt aatttatcta tcatcaatac
      301 ttaggagtga atctatggaa aagccattcg tcaagtcatt gactgctgtg gcggtaggtg
      361 caggtgctat tgcgatctgt ttgtttggat accatatcaa taatcagcgg caacatcagc
      421 aacggatcaa ttatgcagaa tctgccatca ccaatcaaaa agacaccttg acttctttat
      481 caaaagaagt agacaagctg tatagtacaa aagaaaaaat ttttctaaac cctgaaataa
      541 cagaagaaac aatcacaagt ttgtcaaata agctatcctc tgtcaaactt tcagcagatg
      601 attacgacat caaagaatca gaattgccta aagaagctgt tgcaatccaa gatgagaaga
      661 aagcaatcgt gacacagtta gatagtgcag aatcaaaact gaagatccag acagctgtca
      721 acgatttatt tactaaaaat gtatcaaact ggcagcaagc tgtagacgat gtcatcatca
      781 aagaaaaggt gacaagtgca gatgtcgctc gtgtacgtga aaatatgagc ttttttaaag
      841 atagtccttg gaaaacggtt gttatgcaat atctaggatt cgctgatatt cagatcgcgc
      901 aagtcacgca actagatcag ttattcgata cgatgttaaa ggatggtcaa gtgactgcca
      961 ccgctactta cgatcaatat ttgacggtgc tcagtcaaat agagcagatc cggaatgaaa
     1021 aaataagcgc agcatacgca acaaaagcac aaactgttgc tgagcaaatg ggctatagca
     1081 atataagcta ttgacagtta aaacgttgat tcaagcaagg ttagcaaacg gaggaaacta
     1141 tggcaagacg aagaagaaaa tcaagaggga aaaaaatatt tctgattatt ttagcagttc
     1201 ttatcgtatt agtattagca attgtaggag taggagcaaa attatatctg gatctctcac
     1261 attctattga gaaaacttac gaatcagtgg aacgaaccca aggagaacga acgaaaccag
     1321 ttgatctgga caaacaagaa tctttctctg ttctattgat ggggattgat actggagatt
     1381 taggtcgggt cgatcaagga agatcagata cgatgatggt tgccacagtc agtcctgaag
     1441 acaaacagac aacgatcgtc agcattcctc gtgatacgta tgtcgacatc gtaggtcgtg
     1501 acacaaaaga caagatcaac cacgcttacg cattcggcgg agctgccatg gcgatggata
     1561 ccgtacaaaa atatgtagac atcccaatcg atcactatgt ctctatcaat atgaaagggc
     1621 taaaggaact cgttgatgca gttggtggta tcgaagtcaa taatgacctc actttctcac
     1681 aagatggcta tgactttcca atcggtaaaa ttacattgaa cggggatcag gcattatctt
     1741 attctcgtat gcgttatgaa gacccaaatg gtgattacgg tagacaaggg cgccaaagaa
     1801 agatcgtaga aggcgtagcg aaaaaagtat tgagtctaaa cggtgtcagc aactatcaat
     1861 ccgttttgaa tgctctagag tcgaatatga aaacagacat gaactttgat gaaatgaaaa
     1921 aaattgcttt agattatcgg gaggcatttg gtacgattaa gcaagatcag ctgcaaggag
     1981 aaggcttcat gcaagataat atctcctatc aaaaagtcag tgatcaagaa cttgatcgtg
     2041 tgaaaaaaga attaaaagat caaatgaatc tagagaataa ataggaaagt ggagaaaaaa
     2101 tggaagagac aatcagcttg aaagaaattt ttgaaacatt acgcaaacac ttggtaataa
     2161 tcatcatctc aatgtttgct ggcttggcca tctcaggaat cgtaacattt ttcatcttga
     2221 caccaaaata cagttcacaa gcacaattga tcgtgacact gccacaatca gacactgcaa
     2281 atgtcaatga tgtcaatacg aacctgcaaa tgatcaatac gtataaagat atgatcgtca
     2341 gtgatttagt gctcaatgaa gtcaaagatc gtttagaaac agaagacaat gtgaaaatga
     2401 ctgctgaaca aatcaaagat gccatctcag ttaatcaggc agaaaattca caaatgttct
     2461 cgattcaggc aataagtacg aatgcagtgg ctgctcaaca gatcgctaac acaacttcac
     2521 aagttttcca agagaacgca aaagatgtga tgaatgttga taaaatctcg atcatttcag
     2581 gtgcagttgc caatacgaca cctgtttcgc caaataataa attgaattta atgactggct
     2641 taatgttagg aatgatggta ggtgtaggtc tggcattctt attggaatta ctcgatcgga
     2701 cagtgaaaga tgaaaaatat gtgacagata cattaggatt cccaatctta ggaacagttc
     2761 cagaaatgtc agcgaaagaa ttgaatgctg gtatccgtaa aaaaggacca gctcagacac
     2821 aaccaaaagc tacaaacgat aaaacagcgc catcacgcag aagccgctca agagtctagg
     2881 aggaaagaaa atggcacgaa cacagaaaca aaaagcagcc caaactcatg cagtcagttt
     2941 ggtcacactc accaatccct cttcaccaat cgctgaacaa tatcgtacga ttcggacgaa
     3001 tatccagttt gcctccgcag ctggtcagca aatcaaaacg attgttgtga cttcatcagg
     3061 acctggagaa gggaaatcga ccactgcagc aaacattgcc gttgtttttg ccaaatctgg
     3121 tcaacgggtg ctgctagtcg atgcagactt acgtaagccc gtcatctata aaacgttcaa
     3181 gttgaacaac gcaagcggat taagtactgt tcttagttca tcaggaagtg tagcagatga
     3241 gatccaacga acaccagtcg acaatttgtc gattctgcca agtggaccta aaccaccaaa
     3301 tccatcagaa ctactaagtt cgccaagaat ggatcagatt ttagcagaag caagacaact
     3361 atttgatgta gtgatcttcg acatgccgcc agtcgttgcc gtaacagatg cacagatcat
     3421 gtcttcaaaa acggatggca ccttattggt cgtacgggaa aatacgtcac gaaaagaatc
     3481 attgactaaa gcaaaagaac tgttagatat ggtacaagca cgagtcttag gtgtcgtata
     3541 caacggagca gaacacagca aagatgctgg ttattactac ggaaattaag gaataagaag
     3601 agaggagaca agagaaatga tcgatctaca ttgccatatc ctaccaggaa tcgatgacgg
     3661 ggcagaaaac ttagaagcaa gtattgcaat ggctgaaaaa gcgattcagc aaggcattac
     3721 gcatatttta tgcacaccac accacaataa tggaaaatac agcaatgaaa aatcacaagt
     3781 catttctcta gtcgcttcgt tacaagccga attagagaaa agacagttgc ctttgacgtt
     3841 attggaagga caagaagtac gtattaccgg aacgctcatc gaagacatcc accgagatga
     3901 aattttattt acagatttag atgatacata tcttctgatc gaatttccga cactggaagt
     3961 gccgttatat gcagaacggc ttttttttga gttgttagaa ctaggaaaga taccagtgat
     4021 cgtccatcca gaacgcaacg cgcattttcg agaagaccca aatcatttgc ttccgttcct
     4081 agatatggga tgtttggccc aacttactgc gccaagttat gttggtgtat ttggcaaaga
     4141 tatccagaaa acagccaaaa caatggtcaa acataatctc gtgcagatgg ctgcttctga
     4201 tgcacacggg gtctctaagc gcacctttta cttaaaagag tgctatgagc agattgccaa
     4261 ggagtttggt aaggaaaaag tagtacagat gaaacaagtc gcaaaggatc tgatcaacgg
     4321 ggatgagatc aattatccaa tctatgaaga ggtaaaaaag aagaagtttg gtttatttta
     4381 gttctttatt cgtagataaa aaggaaatga ggagagagtg agagatgtat agaaaaaact
     4441 caagtggttg gtctaaacat attgatttta ttttattaga cttattttgt gtccagcttg
     4501 ctttttatat atcatatgta atgcgacagg gtgactggaa tccgtatgtg attcctattt
     4561 atcgccacat ggcaattttt atagaacttg agaatcttac gattatattt ttatttgagg
     4621 catataaaaa tgttttaaaa agaggatatt ataaagaggc tgtagcttct ataaagcaat
     4681 cttttatgct gttacttgtg tgcagtttat accttattac aatgcaggat ggaaatgatt
     4741 attccagagt ttctttattt ataatggggg tggtgtatgg attattaaca tatataattc
     4801 gtatactttg gaaagggttg ttgcatcacc gcatgaaaaa cggtgaagat gtatcgttag
     4861 ttattgtaac atctcaaaag attgcaaaaa atgtagttcg taatcttaaa gagaagaatt
     4921 atggcatgta taaacttagt ggtgtgattg tagtagacag agatatgtat ggaactacca
     4981 tcggaggtgt cccagtagtt gcaaatgctg ataatgcagc ggaatatctt ttacagaatt
     5041 gggtagatga agtattcatc aatgttgacg aaagtgttcc atatcccaaa gaattaatcg
     5101 ccagatgttc ggaaatggga cttgctgtac atatgaatct tacaaaagtg tctgatagta
     5161 cacgtggaaa acaatttatt ggccgagttg gtgattatac tgttattaca tctagtatta
     5221 atgttatgac gatgaggcaa gcatttataa agagaacgat tgacattctt gctggaatta
     5281 ttggatgtat tgcaacaggt gttattttta tcttcgtagc accagttatt tacatcaaat
     5341 caccagggcc gattttcttt gcacaagaac gtattggtca aaatggtaag acatttaaga
     5401 tgtataagtt ccgtagtatg tatatggatg cagaagaacg caaagcagag ttgatgaaag
     5461 aaaataagat gagtaataat ctcatgttta agatggattt tgacccaaga atcattggca
     5521 ataaaatact tcctgatgga acaaagaaga caggaattgg acaatttatt cgatctacat
     5581 cattggatga gtttccacaa ttttataatg tcctaatggg aaaaatgagc ctggtggggt
     5641 ttagaccttg cttgaagtca gaatatgaga agtatgattt tcatcatcgt gcgagaattg
     5701 cgatgaaacc tgggattact ggaatgtggc aggtgagtgg tagaagtgat atcacagact
     5761 tcgaagaggt tgtaaaactt gatgcagaat atatcaggaa ttggagtatg ggattagatt
     5821 ttagaatttt gtttaggaca gtcaaaactg tgcttcatag aaacggttcc atgtaagata
     5881 aagatactat agaaggagta aaacgtgatg gcaagaaaaa aattaaaaat ctgtatgcta
     5941 ggtcataaaa gaattccatc aagagaaggt ggaattgaga tagtggtaga agaattggca
     6001 acgcgaatgg ttgcacttgg gcatgaagtg acttgctata atcgaagtgg tcatcatgtt
     6061 agcggaaagg aatttgacca gcataaaaat aaagaatata aaggtgtaaa attaaagact
     6121 atttttacat taaatataaa aggaattgca gcaatgagtt cttcagtatt tggtggaatt
     6181 agagcagcat tcggaaaata tgatgttgtt cattttcatg cagaaggacc atgtgctatg
     6241 ttgtggttgc caaaactttt tgggaaaaga tgtgttgcaa caattcatgg tctagatcat
     6301 caaagagaaa aatggaacaa gcttgctagt acatacatca tgttagggga aaagtgtgct
     6361 gtaaaatttg cagatgaaat aattgtactt agtgaaagcg tccaaaatta ttttgaagat
     6421 atatatggaa gaaaaacaag gtttatacca aatggcgtta aaaaaattga aataaaatca
     6481 gcaggattga ttacagaaaa gtatggatta acaaaagatt cttatatttt gtttttgggg
     6541 cgattggttc ccgaaaaagg aattcggtat ctaattgagg catttaaaga tgttcagaca
     6601 gataaaaaac ttataatagc cggaggaagc tctgatacag atgaatttgc aaatgaacta
     6661 aaggaattag caaaaggtga tgagcgaatt atatttactg gatttgtaca aggacaggaa
     6721 ttagaagaat tatatagtaa tgcgtatatc tatacacttc caagtgattt agaaggaatg
     6781 ccattaagtt tgttagaagc aatgagttat gggaactgtt gtatagtttc aaatatctca
     6841 gagtgtacgg aagtagtaga agacaaggct atgattttta agaaatcgga tgtaagtgat
     6901 ttaaagataa gattagaaga ggcatgcaat caatcggaaa tggttaaagt tttgaagaat
     6961 caagctactg agtttatttg tagtaaatat aattgggata aaatcgtaca agaaacatta
     7021 aatttgtata ggggaaatat ataatgattc gaatattaca gtgtgtgaat gatatgcatc
     7081 gtgcagggtt ggagacgatg ctaatgaact attataggaa tatagatcga actaaaattc
     7141 agtttgattt tttgacacat cgtccatata aaagtgatta tgatgaggaa ataataagtc
     7201 ttggaggaaa ggtctattat gcaccgagac tttatccaca aaattatcct aaatatttta
     7261 aatggatgga acaatttttt aaggatcatc cagaatatga gattgttcat tctcatattg
     7321 atgcgatgag ttatttacct ttaaaagctg caaaaaaagc tgggattccg atacggatag
     7381 cacatagcca taatacatcg atagatagag attttaaata tcttttaaag caatattatc
     7441 gatgtaagtt aacgtctgtt ttgacacatg aattttcatg tggaaaagta gcaggtgatt
     7501 ttttgtttcg aaatgataaa ttcactttga ttccaaatgc agtggatgct aaaaaatttt
     7561 attatgatga aagtattaga aatgatgtac gaatgaaatt aggaatttca agtcagacgt
     7621 ttgtaatggg acatattgga agaatttcat attctaaaaa tcatcgtttt ttgattgaaa
     7681 tttttaaaga atttcacgct attaagaata attccgtatt attaattatt ggaactggtg
     7741 atatggaaga agaaataaaa aattatgcaa agaaaagtgg catagatgat gatattaaat
     7801 ttttaggaaa tagaaatgat atagaaaaat tttatcaagc atttgacgta ttaatgttgc
     7861 catcattatt tgaaggggtt ccgttagtgg gtattgaagc tcaatttgca gatttaccgt
     7921 gtttcttttc ggagaaagtt cctacagaag ttgctttcag tgataagacc aatttcataa
     7981 gtttaaatca atcggcaaaa gaatgggcga gagagattgc aaatgtagat atctctcatc
     8041 gagattcaga acgttcgatt ttaataaagg cagattataa tattcaaaca gcatacaaaa
     8101 tcttagaatc aaaatattat gagttatttg aattaataca aagaggttaa agaatatgac
     8161 acagaaacat tcaagtataa aaagaattat acatcgcata tccgaagaat tgaatgcaac
     8221 aagacgaatt gcaaagataa catcttggaa ggaagcattt gccacgttac aagctaaaat
     8281 tgatattcaa gtcatgaatc gtaatggata taatgaaagt aaaaaacaga aggaacattt
     8341 attaaaaaaa catgaggtaa tgctgaagta ttatgaaaag actttttcag attttttgaa
     8401 aacatatgat tatagtcaaa atcaaataat taatcaatca aaatcaccat attctaactg
     8461 catttgggtt tgttggtggc agggattgga tcaatcgccg gaaattgtaa aagcttgtgt
     8521 aaaatcaata aaacgaaatg cagggaaaca tactgttata attttaacag aagataatta
     8581 tagacaatat gttgatatac caaaatgggt ggaagaaaag aaaaacaaag gaatcattac
     8641 gagaacaaat tattctgatt tattaagatt atctttgcta gcaaaacatg gaggtttgtg
     8701 gttagacgcc acattttatt gtacaaaacc aataccagat gagtattttg atatcgatat
     8761 ttggtctatt aggaggcctg attatgctca cgcatccgta gcatcaggat attttgcggg
     8821 ttattcatta gcatgtaatc aagagtctag atggatgttt gctataatta gagacttttt
     8881 tttaaattat tggaaaaata atgatatgat ggtggattat ttgatgatag attatatgat
     8941 tgttttagca cagagaataa atcatgaaat agcacagaca tttgataaaa tacctagtaa
     9001 caatccatta tgtgacgaat tgattaaaaa tttaaataat atatatgatg aaaaagtatg
     9061 gaacttatta aagaaagata ctttactgtt taagttgaca tggaaacaat cttttaaaaa
     9121 gcaaaaagat gataaagtta ctttttatgg gatgttggta gaaaacaaat tattataaat
     9181 attagaggta taaaaagtta tgaaacacaa ggtaagtgtg atagtaccaa tttacaatgt
     9241 tgaaaaatat ttaagaaaat gtgtggatag tttattgagt cagacattaa aagagataga
     9301 aatcgtatta gttgatgatg gttcgccaga tgcttcgggg gaaatagcag atgagtatca
     9361 aaaaaaatat tctaatgtaa aaacaataca tagagaaaat ggggggttag gaccggcaag
     9421 aaatacagga attgagaatg cgacaggaga atatgtggct tttcttgatt cagatgattg
     9481 ggtagaaagt gatatgtatg aaaaattata cttagttgcg tccaaagaaa atgctgatat
     9541 agtagttagt ggacattgtg attttgcgaa tgacaaagct atggtgaaaa agcaacatcc
     9601 tttgagtggg aatgtatatg atacgcctaa tagtattaaa aatgtacgaa aaaatttatt
     9661 tggacatagt ccaaaagatc aagaagtaga ggccttcccg atgtcagtat gtatgtcttt
     9721 gtataataaa aatctgctag ataaatatag actaagattt gaaaaaattt tatctgaaga
     9781 tacaattttt aacttgaatg catatgatgt cgcaaataaa attgctttta cagaatatac
     9841 agattattgt tatagaaaag aagaacaaga gtctattaca aaaagtttat ctccaaataa
     9901 gcagacggaa tttaaagaat ttttaataac tctaaaagct atagcatcga aagaagattt
     9961 agaatgtcaa atcagagcaa aacgtatggc aattgattat tgtaggcttt atgtgggctt
    10021 agttgatgat gcaaatttgg gaattaaaga gaaaagaaag tatataaaaa aatttattga
    10081 agatgaggga ttaattcaat gttggaaggg atatcctaca aatacgctac catttcaaca
    10141 aaaatttttt cataaaatgc ttgaatgtaa gtgttataca atattgttgt tgttaagtag
    10201 aattagaaag ataattaaaa atatgaccag aaaaaaattg tttataatat tataaactaa
    10261 aagtatatca aataatagaa attaacaagt tacaaaaaaa tattataaag aataatattt
    10321 tctaaaaagt aaactatagt aatgtggatt atcaccttcc aacagtatat aaaatgagcg
    10381 taaaaacggc tatttttgcc ttaacaacga tttttttgta tacaaatacg atatgctcat
    10441 taattattgg aatttgtagt gctgtttaaa ttaatgaagg tgtaattata tgagtgatca
    10501 ttttgaaaaa gtttgttgag aagatgtgct tctagtcatg tgatgggaga aaataattta
    10561 actgttagag tgaatagtta aattattctg gttatatgaa aaaagtagtt tcta
//
LOCUS       DAZULO010000091        10551 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000091 DAZULO010000000
VERSION     DAZULO010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.093"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(251..736)
                     /locus_tag="ACG8OA_002317"
     CDS             complement(251..736)
                     /locus_tag="ACG8OA_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFS4977065.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    826..1171
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1216..1857
                     /locus_tag="ACG8OA_002318"
     CDS             1216..1857
                     /locus_tag="ACG8OA_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4977066.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1876..2511
                     /locus_tag="ACG8OA_002319"
     CDS             1876..2511
                     /locus_tag="ACG8OA_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4977067.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2529..3344
                     /locus_tag="ACG8OA_002320"
     CDS             2529..3344
                     /locus_tag="ACG8OA_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4977068.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3663..5099
                     /locus_tag="ACG8OA_002321"
     CDS             3663..5099
                     /locus_tag="ACG8OA_002321"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4977069.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5288..7735
                     /locus_tag="ACG8OA_002322"
     CDS             5288..7735
                     /locus_tag="ACG8OA_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFS4977070.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7965..9032
                     /gene="rlmN"
                     /locus_tag="ACG8OA_002323"
     CDS             7965..9032
                     /gene="rlmN"
                     /locus_tag="ACG8OA_002323"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFS4977071.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            9022..9228
                     /locus_tag="ACG8OA_002324"
     CDS             9022..9228
                     /locus_tag="ACG8OA_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977072.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9225..10328)
                     /locus_tag="ACG8OA_002325"
     CDS             complement(9225..10328)
                     /locus_tag="ACG8OA_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4977073.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         3491 a         1803 c         2288 g         2969 t
ORIGIN      
        1 taaatatgac tgaaatcaaa agaataaaat tccgatttat aatcgaaaaa agaagcattt
       61 ttatatatgc ttcttttttc gtagaatcaa taaatatgga tttggctaac tatggaagaa
      121 tactcgtatt agttttcagc gttcatacta gttgttacat tgtttgattg gccaaaagaa
      181 tttctgatat attttgtgct aaataaagaa aaagacgctt gaattgggaa ataagaaaac
      241 tactctatca ttacaaattt tcataaactg gcagatgttc taagtaaaca agtatctttt
      301 caaagtcgga aaaatccata atcaacgtag tggtattgat atttggatga aagcctacgg
      361 tatcttgacg atcaatagca gcatcgatca cgacttggat ttttttctct ttgtcgtgca
      421 tcaatgctaa tggagtcact gtacctgctg gaacgcctag gaggtcagga agctgttctt
      481 cagaaacaaa agataagcgc ttttctttta attgttcagc tagttgtttt aaatcggcgt
      541 gcttttcatc aggcaagatg acaagataga tttgttttcc ttttcttgat ttcaataata
      601 gattttttac ttgcggacca ggtagggaaa aagggacatt tttgactgac gtaatcgcag
      661 gatgatcgac tttttgaaaa gagataccca gtttttttag tagttcataa gcttcttgtt
      721 cggttgcacg actcatcgcg catcaccctc ctttttttat tagtgtatca taaaaaagag
      781 ttgacaaatg aaaaataatc atgtaaatta cacgtaaagt taaaacaaat gacatcgaaa
      841 agaagagtag ccgttaggtg tttttagaga gtctctggtt ggtggaaaga ggcagcagtt
      901 aaccgtgaaa cacatcttgg agtcgatttt ctgaagcgta tagtaggaaa atccggggag
      961 cccgttacag ctgcggtcgt tgctagatga ccagataagg ctggcttttc cgtgaggagt
     1021 cggtaaaaaa aggtggaacc acgtgattaa cgtcctttga tcttcggatc aaaggacttt
     1081 ttgttttcat ctgaacggca acgactgtgt gaggtttttt ctgcaaggaa aaaataaaca
     1141 gaggtggaac cacgtataat acgtcctctt ggcatttgcc aagaggattt ttttatttta
     1201 aaggaggaaa agaagatgga gtatttgatt gaagttatgc cgcaattatt agtaggagca
     1261 ctcacaacat tgaaattatt tttatttacc ttgattggct cattaccatt agggattgtt
     1321 ttagcattag gattgtcttt gcattttaag ccgctacgct acttgcttca aacatatgta
     1381 tgggttatgc ggggaacacc gctgttgttg caactgattt ttatttttta tgggttgccg
     1441 acgattggga tcgtttttga tcgtttcgaa gcagcactca ttgcctttat cttaaactat
     1501 gcagcttatt ttgcagagat ctttcgtgga gggatacaat cgattcctca aggtcaatat
     1561 gaagcagcgc aagttttgca attaagtccc tttcaaacgg tgacgaaaat cgtcatccca
     1621 caagtgacaa agattgtttt accttctgta gggaatgaag ttatcaactt ggtcaaagat
     1681 tcttccttga tctatatatt aggtttaggt gacgtaatgc gtgctgggaa aatcgcgatg
     1741 gaacgcgacg cgactttact gcctttgcta ggcgtggcaa tcatctatct tacattgact
     1801 ggtattctaa caatcataat gaaacaagtt gaaggaaaat taaattatta ccgctaactg
     1861 ataggaggaa gaaatatgat tgaattgaag aaagtgaaca aagcatttgg tgaaaaaaaa
     1921 ataatcaaag atttgaatct aatcattcca gatgggaaaa cattagctat cgtaggtcct
     1981 tccggtggcg gtaaaaccac gcttttacgt atcttggctg gcttagaaag ttctgacagc
     2041 ggagaatttt atttagacgg tgtaccgttc aacccattcg agcttaaaat gaaagaacaa
     2101 gttgtaggag tcgtattcca agacttccag ctatttccac atctgaatat ttttgaaaac
     2161 attaccttag caccaaaaat ggtattgaaa cagacaaaag aagaatacac caaaaaagca
     2221 gaagaattag cggcttcatt agggattagt gatttattga atatctatcc ttatcagctt
     2281 tctggcggac aaaaacagcg ggtagcgatt gccagagcat tggctatgaa tccacgggta
     2341 ttggcttatg atgaaccaac aagtgcttta gatcctgaat tacgccagca agtcgagcgt
     2401 ttgatcctat cattgaaaga agatggggta acccaaatta ttgtcaccca tgatttagta
     2461 tttgcagaaa atgtcggtga tcaattacta aaagtttccc caattcatta agaaaggaga
     2521 agatagagat gaaaaaaacc agttttctat tcgctttgat cgcaggtctt cttctttttg
     2581 caggctgttc gaacaaaaaa acttcagcag ataattgggc gactatcaac gaagagaaaa
     2641 aaatcgttat aggtttagac gatacatttg ttcctatggg atttagagat aaagacggga
     2701 agctcacagg atttgatatt gatttagcca aagcagtctt tgaacaatat gggattacgg
     2761 tagactttca gcccattgac tggtcaatga aggaatttga actgaacaat ggaacgatcg
     2821 atttgatatg gaacggctat tccaaaacgc cggcaagaga aaagaaagtg caatttacaa
     2881 aaccttatat ggaaaacgat caagtgctga tcacacctaa agaatcagga atcacaaact
     2941 ttggacaaat gaataataag agattaggtg cgcaaaatgg gtcatctggt tatgatgtat
     3001 ttacgaaaca accagaagta ttaaaagaga tagtcaaaaa ccaagatgct gttttgtatg
     3061 atagttttaa tgaagcattg atcgatctga aaagcagacg ggtcgatggt ctgctgatgg
     3121 ataaagtata cgcagactat tatttgaaac aaagaaacga actagaagac ttcaacataa
     3181 caaaaggttc ttatgagagt gaggattttg cagttggtgc acgcaagtca gatagtacat
     3241 tagtgaaaaa aatcaatgaa gccctagatc aactagagaa aacaggtgaa ttccaaaaaa
     3301 tatccgataa atggtttggc gaagatgtta cgccgagaaa ttaacaaata ggggactgtg
     3361 acaaaagacg tgtcacagtc cctttttccg aataaacagt gttctcctgc caactccttg
     3421 tgactcggag ctgaatggca ggctcacaac ctcttggtaa acggtgttca aaagccagtt
     3481 tttcggtatt aagccaaatt tatgcaaaaa tgtagaaagc cattttcaca aaatttttct
     3541 tactactcga aactaagcga ctttttcaca acctccttta cataatttta ttttgttgtg
     3601 gaaggggttt cgtgttattc taaaagagga acgattgaag gttataggaa aggaagagat
     3661 gaatggaaca caaagaactt acctcatttc caaaagattt tctgtggggc tctgcttctg
     3721 cagcgtatca agtagaagga gcttggcaag aagatgggaa aggagaatca gtctgggatc
     3781 gatttgtacg gatacctgga aagacattta aaggaacgaa cggtgatttg gcagtagacc
     3841 actatcatcg ttataaagaa gatattgctt taatgaaaga acaaggtctg aaagcttacc
     3901 gcttctctgt agcttggaca cggatttttc ctaatggacg aggagaagtc aatcaagctg
     3961 ggctcgcttt ttatgagaga ttgatcgacg aattgattga aaacgatatt gaacctatgt
     4021 tgactttata tcattgggat cttccacaag cattgcaaga tgaatataat ggttgggaat
     4081 cacgtcagat cattgaagat ttcactcatt atgcagaaac attatttgag gcattccgcg
     4141 ggaaagtcca ttactggatt agtttgaatg aacaaaatat cttcacttca ttaggttatt
     4201 tgttagcagc acatccgcca ggtgtgacag acccgaaacg aatgtatgaa gttaaccata
     4261 ttgctaattt agcaaatgca tctgtcatca ataaatttca cgagttagaa atgcctggaa
     4321 aaatcggacc aagctttgcg tatacgccaa attatccaat tgacagtaat cctgaaaatg
     4381 ttctggctgc tgaaaatgca gaagacctga tggccaatta ttggatggat gtctatatgt
     4441 ggggaaaata tccgattgct gccatgaggt tcttagaaga aaaaggatgg gcaccaacga
     4501 ttgaagctgg cgatgcagaa ttgctagaat ctgctaaacc agattttctt gggatcaatt
     4561 actatcaaac ggcgacgaat gcattcaatc cattaaacgg agttggtgct ggaaaaatga
     4621 atacgacagg aaaaaaaggt tcatcagaag aaactggagt tcctggaatg tataaaaaag
     4681 ttgagaatcc atttgtcgaa cgaacaaatt gggactggga gattgaccca gaaggattaa
     4741 ggatcggact gcgtcgtatc acaagccgtt accgcatgcc ggtactgatt acggaaaatg
     4801 gcttaggaga gtatgacaag ttgacagaag ataaacaaat ccatgacgac tatcgaatca
     4861 attacttaca aagccacgtc aaagccatca aagaagcaat ttcggatgga gcagaagtcc
     4921 taggctattg cacctggtct tatacagatt tgcttagctg gctgaacggt tatcaaaagc
     4981 gatatggatt tgtatatgtt gatcaagacg aaacacaaga aggatctttg gaacgatata
     5041 agaaagacag ttattactgg taccaaaaac tgattgaaga aaatggagag aacctttagt
     5101 tcacatactt aaaacaagaa ggtacggctg gatatggccg tgccttttct tgataaaatc
     5161 atttagaaat taagaaaaac tgtaaaataa gctgaaaaat gaaagaaagc gtcagttctt
     5221 tcaaacaaaa ctttagttcc cttacttttt tttgtataat gaaacttgat gaaatgaggg
     5281 ataataaatg gcacaaaaga aacggcctac taaaaaaagg aaaaagacga aaaaaacgaa
     5341 aaagcaaaaa cagcagcagg aacaaatgct gacgattggt gttgggtttg ccttgatttt
     5401 atttgctgtc ttcggctttt taaaattagg ttttttagga atattgattg caaacggctt
     5461 tcggatcatt gcaggaaata cttaccaaat cctctgtctt ttattagccg tattaggatt
     5521 ttggattgtc attaagaata cagagttttc aattggtaaa aaccgacgct ggtttggcgg
     5581 catactcttt tatttcggta tcttattatt gttacatgcc catttatttg gcaaactcca
     5641 tactggggaa ccaaatatca tggggacgac atgggatttg ttagccagtg atataaaaca
     5701 aagtcaagta gacaataacg ttggtggtgg gatgattgga gcaatccttt atcattttac
     5761 ttacttcttg attgcacagc ctggaagtta tttagcagca gtgctattac tggcaggagg
     5821 agcattcctg ttcagtaatc tggaaggcta tcagctgtta aatggtatcc aatcaattgg
     5881 agaacgagta caggaattat tggaaggtga tcctgccaag caagctcgaa agcaagcggc
     5941 aaaggaagag agaatgaaac agcgcgcaga agcgaaagaa gcaaggcgct tagctgcaaa
     6001 agaagctgca gaaaaagaag ctgcagaata tgagaagaaa aaagcggtga gccagcaaag
     6061 ggaaaatact gctgatgaat ggcaggaacc agaaaataca gagccggagc agctaagttt
     6121 tgttccaatc gacagttttc aagaaaatat ccatcctgcg aatctagaga aaccagtacc
     6181 agatacacca aaacagacca atacagcaga aggtttttca gatgagttgc cagaggatga
     6241 cggtacctca ttagaatttg agattgaagc agaacaggaa aatcaagact atgaattacc
     6301 gacagttgat ttgcttgatt ctataccaac tgtggatcaa agtgatgagt ataaaaaaat
     6361 tgaaaaaaat atcggcgtat tagaacaaac tttccaaagt ttcggtgtcg atgccaaggt
     6421 cgtcaaagcc agcttaggac catccgtcac aaaattcgaa gtccagccgg ctgtaggggt
     6481 aaaagtaagt aaaattgtga acttaacaga tgatatcgcg cttgctttag cggcgaaaga
     6541 tgtccggatg gaagcaccga ttccaggtaa atcactgatt ggtattgaag taccaaatgg
     6601 aaagatcagt atggtttcct ttagagaaat catcgaagca caaccaaacc acccagataa
     6661 attactagaa gtacctttag gacgtgatgt ttctggacgg gtacaaacgg ctgatctttc
     6721 gaagatgcct cacttactcg tggctggttc gactggtagt gggaagtcag tagcaattaa
     6781 tgggattatc acaagtatat taatgcgtgc taagccccat gaagtaaaac tgatgatgat
     6841 cgatccgaaa atggttgaat tgaatatgta caacggcata cctcatctgt tgacgccggt
     6901 cgtgacgaac cctagaaaag cagcgcaagc cttacaaaaa gtagtacaag aaatggaaga
     6961 acgatatgaa aaattcgcgg caaccggagt aagaaatatt tctggttaca atgaatttgt
     7021 tcaacagaaa aacttggaaa acggaacgaa acatccaacc ttaccattca tcgtagtgat
     7081 cgtagatgaa ttagctgatt tgatgatggt agccagcaat gaagttgaag atgcgattat
     7141 tcgtttagcg caaatggctc gggcagcggg gattcatatg atcctagcta ctcaacgtcc
     7201 aagtgtggat gttatcacag gaatcatcaa agcaaatgta ccttccagaa tggcatttgc
     7261 ggtatcaagc ggtacagatt ctcggacgat cattgattct aatggggcag aaaagctctt
     7321 agggcgagga gatatgctat tcttgccaat gggtgagaat aaaccgatcc gtgtgcaagg
     7381 agcattcatt tctgaccacg aagtagaacg ggtcgttcaa tttgttacag atcagcaaga
     7441 agctcattat gaagagaaaa tgatgccgac agatgaagtt gaaacgacag gtgctccaga
     7501 acagccacaa gatgagctgt ttgaagaagc aaaagcactt gtcgttgaaa tgcaaacggc
     7561 aagtatttcc cttctccaaa gaagatttcg gatcggttac aaccgagcag cacgtttagt
     7621 agatgaactg gaagctcatg gagttgttgg cccgtcagaa ggaagcaagc ctcggaaagt
     7681 attcattgag caggaagaaa catcagaaac gttgccggac gataatgaca tgtaaaactt
     7741 gtgtgatgaa gggttgaaaa acgaataacc aataaaaaga caaggcatga tgataccaaa
     7801 agaaaaaatg gtttttttcc atcttttcta atacggtatg atgtcttgtc tttttagttt
     7861 ttgtgagatt aaagttctat tcgatttatt tgtgaagtat tgcattaagg aaatgaatca
     7921 tgtatagtat gtagcgaaac tttgagaaaa agagggaaaa tactttggaa aaacagtcca
     7981 tttatggctt gacgaatgaa gaattaatca attggtttat agaaaacggc gagaaaaaat
     8041 tccgagctgc ccaagtttgg gaatggttgt atcaaaaacg tgtaagtaat ttcacagaaa
     8101 tgacgaatct atcgaaacaa ttgatcgaaa aattatcgga gcattttatc atcaatcctc
     8161 taaaacagat ggtcgtccaa gaagcttcgg atggtacagt aaaatatttg tttgaactcc
     8221 ctgataaaaa catgattgag actgtactga tgagacaaga atatggcttg tcagtttgtg
     8281 tgacgacaca agtaggatgt aatatcggct gtacgttttg tgcaagcggg ctgctcaaaa
     8341 agaaccgtga tctgactgct ggagaaattg ttgcacagat catgatggtc caacattatt
     8401 ttgatgagcg caatcttggc gaacgcgttt cacatgtcgt tgtaatgggg atcggtgagc
     8461 catttgataa ttacgacaat gtaatggact ttttgcatat catcaatgat gccaaaggat
     8521 tggcaattgg tgctcggcac attacagttt ccactagtgg attagcacac aaaatcaagg
     8581 aattcgctaa taacggcttg caagtcaatc tggctatttc cttgcacgcg ccgaataatg
     8641 aggtgcgaac atcgatcatg cggatcaacc gaagtttccc aattgagaaa ttgatggaag
     8701 ctgtggatga gtatttagag aaaacaaacc gtcgaatcac gtttgaatat atcatgttga
     8761 atcaagtaaa tgatagacca gagcatgcgc aacaattagc agatctatta aaagataaga
     8821 aaaaattagc atatgtcaac ttgattccat ataatccagt cagtgaacat gatcaatatt
     8881 ctcgcagtcc aaaagctgat gtattgagat tttatgatgt attgaagaaa aacggcgtaa
     8941 actgtgtgat acgtaaagaa catggtacag atattgatgc agcttgcgga caattgcgaa
     9001 gcaagcaaat gaaaaaaaca gttgccaaat aatcgtatcc tactcgtttt ttcaattacc
     9061 ataaaaggag cagttttctt aattaaggaa ctgttcaact taaataagag aaaagattct
     9121 atagcgaaag ctataaagct ttttttgaca agaaaaaccc tcatcaagca aagaaatgtc
     9181 ctcaatttga tgacagcctg cttgatgggg gttttagcaa ttatttaact gattttctta
     9241 tatccttgct ggctttcttg tgtagcatga taagtttcta cgtcaagaat ggcctgttta
     9301 aatttttctc gggtgatttc ataaggcaca tgtttaatat catttgttgc catggcttta
     9361 tctaagattg cttctaaatc ggtcgtgaca ttgagattca tgtcggttaa cgatactgga
     9421 agttggttct ttttcataaa cacatagatt ttatcaaaca aatcgaactg ctgatccatc
     9481 atcaataagg tcagtacacc ataactgaca gatgctccgt gaaggtatcc ctccattttt
     9541 tctagaacag tcgtaccgta atagaatgag tgtgccatag cagaattata gttattgtta
     9601 actaaaacag aaaccaatcc ggtagtaaca atgatatccg aaacgacttg ttcaattgca
     9661 tatgagggac gatcgtcggc actatctttc aatgcttgcg taccataagt caagagacga
     9721 tcaatgcaca tttttccgat ttggattcct aatctatccg tgtagcctag atcatcgcca
     9781 cgagcagaaa aagtagattc gacttctttg gataaagcat cgccaatacc tgcccataag
     9841 tatttatcag gtgcttcggc aataattgct gtatcaatga aggtgtgtac aggtggtttt
     9901 gaaggaaaga agagtccgtc cattacgcct tcttcgttat agatgacaca taccgcagtg
     9961 acgggcgaac agttcgaacc aatagtggga aaggtgaaga ctggtttagc tagtttttca
    10021 ccaatgactt ttacagtatc gcaagcacgg ccaccaccga ctgcaaacag gacgtctgct
    10081 ttttggacac tttcatgctg gatcaactgt tcaatatttc cataagttga attacccccg
    10141 taccagagac tggcgacaac ctctatttcc tgtgtcaaag ctacttttat tttgttttct
    10201 gcctttgaaa gtgcagtctt ccctccaatc agcactactt ttgtcccata ttctttgacg
    10261 attccatttg ctttttcata cgcatctgtt ccaatggtat aatttggtaa gaaaacactt
    10321 tgactcatat tcatttcctt ctttcttcgt tgaaacgggt taaaatttta aagagtgatc
    10381 gatctgactg cggctgtaga tggagtagag gtgaacctaa gacaggataa gtgatgaata
    10441 aggtggcatg aacaccttga aatcatcaat aagtatccta aaaacgaaca agtacaaaaa
    10501 cattgaaaac gacaataatg aatttttgtt cctcttagta tttctaccga t
//
LOCUS       DAZULO010000092        10327 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000092 DAZULO010000000
VERSION     DAZULO010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.094"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..277)
                     /locus_tag="ACG8OA_002326"
     CDS             complement(<1..277)
                     /locus_tag="ACG8OA_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFS4977074.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCK"
     gene            605..1084
                     /locus_tag="ACG8OA_002327"
     CDS             605..1084
                     /locus_tag="ACG8OA_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFS4977075.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            1117..3345
                     /locus_tag="ACG8OA_002328"
     CDS             1117..3345
                     /locus_tag="ACG8OA_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4977076.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3474..5117
                     /locus_tag="ACG8OA_002329"
     CDS             3474..5117
                     /locus_tag="ACG8OA_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS4977077.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(5190..5735)
                     /locus_tag="ACG8OA_002330"
     CDS             complement(5190..5735)
                     /locus_tag="ACG8OA_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFS4977078.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5758..6243)
                     /locus_tag="ACG8OA_002331"
     CDS             complement(5758..6243)
                     /locus_tag="ACG8OA_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4977079.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(6233..7045)
                     /locus_tag="ACG8OA_002332"
     CDS             complement(6233..7045)
                     /locus_tag="ACG8OA_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFS4977080.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(7184..8716)
                     /locus_tag="ACG8OA_002333"
     CDS             complement(7184..8716)
                     /locus_tag="ACG8OA_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFS4977081.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8825..9856)
                     /locus_tag="ACG8OA_002334"
     CDS             complement(8825..9856)
                     /locus_tag="ACG8OA_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4977082.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3427 a         1910 c         1979 g         3011 t
ORIGIN      
        1 acttacattt aaatcgttgt ttcttcagct ccagataagt gcgatactca gacacatcat
       61 tcagtaacaa ccgaacggtt gtcgtccccc aacgaatgat cctagagggg acgacacaaa
      121 gacaatggga acactcttct ggcttgtaca gaagagttcc tttgtaaatc aagcaaattt
      181 gccctttgta tttttcttta gttaaacaat tttcctcgaa atagatattt aggtctagaa
      241 tatttagggt atctttgata agctgagtat gagacatctt gaatcctcct atgaattggt
      301 agccaaatta attctaaagg atttcagggt gtcttttcta tattttttta tacaaaaaag
      361 atgttggtga agttcaccaa catcaaaaag tatagagcca aaatattcca acatcatctt
      421 ggactttcta catgttacta atgagcgtgt aaaagaacga gatggtgttt ttatttctat
      481 caatgaagct ctttttcttt gcaatcctca cttcaatcct ctataatgtc gcatatagtt
      541 tagtaaatga gtaaactact aatttactta tttactaaaa ataaaaggat gaggagttaa
      601 aataatggaa ataccaacaa atttaaaaca taaaccagta atcgttgtag aagactatga
      661 taaagttgat ggaagaaatg cattaaatat ggatgcaaaa gggctttcac tgggccttgc
      721 tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag gtatggcgac atactggaga
      781 aaaatggtca cgacagtcgg aagaattacc aatcacacgt attttagatt tagcgatctt
      841 agctgcgcaa ggaagtttat acttccaaga tgcttatcgt tatgaaaaat tttatgatcc
      901 agaaaatcca gtcgtagaca tcattggatt acaagggaac cgaatgacgg tagaagtaga
      961 taccaaaaat ccaaagatcg atgaagacat cattttgtat tacgatacac tacaaaaaga
     1021 cggagaactg attggggaac gattccgtat tttaaaacgt cttttagatg agctaggata
     1081 ttaatagaca gagatgatca aaaggggaag agaagcatgg agaatttctc actaacagaa
     1141 aaccgaacct tttcacggct agaaaagcaa atgatctgga agaaaccgaa aagccatcgg
     1201 acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag ggaattggca agatcctgaa
     1261 atgaaaatca gtaacgtact tttggaagga gatgccgggt cggggaagac acaattagcc
     1321 aaagcactat ctgctgatct gcaacttccc tatacaaaag tgacctgttt tgcagatatg
     1381 gacaaatcag atgttttcgg agccttatta cctgtagtag attctaataa ggaacaagac
     1441 caagaattac tagaagctat ttaccaaaca gatacacttg aagctgtact gacactgatc
     1501 cagaaccatt acggcattga tcctttttct gctaaagaaa aattagcaga gctagttcaa
     1561 aggatcgaag ctgattatcc agatgcaatc cattacagct attacccttc tgaaatcgtt
     1621 cgagcgattg aaaaaggcta tttattagag attcaagagc cgactgtcat ccgagatgcc
     1681 tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg ggatgttgaa tttaccgaca
     1741 ggaatcattc gtcgccatcc agattgcgtc attgtgatca ctacaaatcg aaattaccaa
     1801 ggaaatcgcc cattaaatga atctttaaga gatcggatgc aacatgcgga aaaaatggat
     1861 cttcctgcct tagaagtcat ggcagaacgt gcgatggcga aaactatgat ccaaaaacaa
     1921 gaacttctct taaagatggc agagattatc cgtttgttgg atgaaactgc aaaagctaac
     1981 gccatcaaag gtgtagcagg aatgcgttct tatttttatt gggcaaatac ctataagcaa
     2041 ggacaagatt tactccagtc gatttatcca aaagttttgt ataaattatc cactgattca
     2101 gatgaattgg caattcttga acaagctttg tctgacagtg gattgctact ggaacttcag
     2161 gacatattga gaaaagaaaa atggggtcat cctcaatctg agcatacaaa agaaagaacc
     2221 attagcgccg aagaagcaaa tgaacgaaac atcactgaaa atgctgaaga aattctgcgc
     2281 ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg agggagaaga gaaacaagaa
     2341 gaaacaatag aaaaaaacga gattcctcct caaatgcaaa aagaacgcag tagcgatatc
     2401 gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt ctcaagaaat gtctgcttct
     2461 gacatggaag cacatgataa ggaaatcaag aagcaactga ataaagaagc gcgacaaatc
     2521 atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc accgaccagc tttccaagga
     2581 aaaacgccgg aagcacgtga gctacacatg caagtaatgc caatagtaga atcacttagc
     2641 cggcaaattt tagagttact ggataatgaa caaacagaga cgtaccaaaa aggaaaatac
     2701 gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg atttgagaaa ttttgacaaa
     2761 aagaacccgc cacatgagca accttcttta gcggtagctg tacggatcga tgaatctggg
     2821 tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag cagcgattgc cattgccgaa
     2881 ttttctaaga aagtcgatat cccattgttg atttacggag atacagcaga ccgttcgtca
     2941 agagaaaaga cttctttgtt ttcttataaa gaatttgaag acggatttca atggttaaat
     3001 gaaaaattag tcactatgaa accaaggcaa aacaatcgtg atggtgctgc acttcgttta
     3061 gcagcagaaa aattaaacag acaatccgca acaaccaaac tgttgcttaa tattagtgac
     3121 ggtcagccaa aagcattgcc ggattataca ggcaaaaaag caaaagaaga tatccagtct
     3181 gttttgaaag aatatgaacg tcaaggaatc ctatttgtct ccgcagctat tggccaagac
     3241 aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg tagatattac tgacttgaat
     3301 gaatttccca agcaaatgat tcaactgatt gcccgctatc tatgataaaa taaagctcaa
     3361 cgaacgcact tagcagattc gttgagcttt tcgtttctag ttaccggtaa caaggatact
     3421 tctaaaatag aatcggttat aatcaagagg gaccgttaaa ggagtgaaac gatatgatac
     3481 aagaacaaaa atggtggaaa gaagcagtag gctatcaaat ctatcctgct agtttcaaag
     3541 atagcaatgg cgatggtcaa ggagacatca atgggatccg tgaaaaactt aattatttga
     3601 aagatcttgg tatcggcttt atctggatca atccgatcta tcaatctcca tttgtcgata
     3661 atggttatga cgtaagtgac tatcaagata tagaagagag attcggaacg atggaagatt
     3721 ttgatttatt gctaaaagaa gcacatgaat tagggatcaa agtgattatg gatcttgtca
     3781 tcaaccatag ttcggatcag catcaatggt ttgaagaatc gaaaaaatct aaagaaagcc
     3841 cgtatcgcga ttattacatt tgggtcgacg gggtagatgg caaagaaccg aataattgga
     3901 catcgatttt cggcggttct gcttgggagt attctcatga aactggacag tattatctgc
     3961 atgtttttgc aaaagaacaa ccagacttaa attgggaaag cgagaaattg aaagaagaac
     4021 tattcaatat gatccgatgg tggttggata aagggataga tggatttcgt ttagatgcga
     4081 tttctcatgt aaaaaaagat gaatattcag taaaagccac agagaaccct ttctcaccct
     4141 ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt gaaacatgtt tttgaagagt
     4201 acgatatcat gactgtcgga gaagcaagtg gtgtcactgc agaagaagga ccacaatggg
     4261 tagggaaaga cggttatttc gatatgatat tcgaatttga tcatatccat atctggcagc
     4321 aagaaaaaga agggcaatta gatgtcctta aattgaaaca tgcattaagc gcttggcaaa
     4381 cttcattgga tggtatcgga tggaacgctt tatatatgga aaaccatgat gttcctcgtg
     4441 ccgtttcagt gtttggagat actcgtcccg atttttgggc tatgtctgca aaagcaatcg
     4501 ctatgatgta tttctttttg caaggtacgc catttattta tcaaggacag gaaatcggaa
     4561 tgacgaatat gccctttgaa tcgatcgatc aagttgatgc agtagattcg aaaagacttt
     4621 ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc attggatatc attcgtgaaa
     4681 caacgagaga caacagcagg acacccatgc aatggacttc ggaacaatat gctggttttt
     4741 ctacacatga accttggctg atcactaatc caaataccaa aacgatcaat gtagaacaac
     4801 aagaatatga accagagtcg gtcctgcaat tctataagaa catgatccgc attagacaaa
     4861 ccaataaagg tctgatatac ggttcttata aagaatatct tcatgaacat ccacaacttt
     4921 atgtctatga aagatattta gaagatgaac aatatcttat tatggtaaac ttaacagaat
     4981 cacttgctga ctatgaactt ccaaaagaag cagatcagtc ctggacatta ctcttgtcaa
     5041 attcctctag cggagaattt gaagctaaag gcatactagc accttatgaa gctcggttat
     5101 ataaaacaaa caagtaaaac ttcctcgtta aagttcatta gttggacttt gcgaggaagt
     5161 ttttcagatt gctctgtcaa ttactcattt caataattcg ctctaggacc accgatatat
     5221 ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa gggttttcaa acgaggtctg
     5281 acaggtactt ggacatgctt gatatgcata ccgattgatg tgtcaccgat atcgataccg
     5341 gcttgtccaa ctacgtgttc cacttcgaca ggatcattga actggtcaaa agcagcaaca
     5401 gaacaagcac ctccagcatg tagtgcagga acaacagaga caatttcgaa attttttgct
     5461 tctgccactg ttcgttctac tactaatgct cggttcaaat gttcacaacc ttggacagct
     5521 agcgcaattt ctttaggatc caacaactct tttaacgtac ggatgatcca ttgaccaact
     5581 tccgcactgg aattttttcc aatatgtcct ccaacgactt cgcttgtgct gcatcctaaa
     5641 acaaaaagat cacctttttt caagtgtgcc tcagccaata tgtcattaac tatttcagtc
     5701 aattggtctt ttaattcttt ttctgacaac ttcattttct aaaaccttct ttttcaatta
     5761 atattgcttc cgtgcttgaa tacgttgaag acttgcagtt ataggaaagg caagtcccat
     5821 tccaatcaga ttttggaaga tgtttcctgg tatagaagcc actccagccg gccaatcata
     5881 taaaatccaa ccggcgatac catagccgat gatcataact aagcttccta ataaaagccc
     5941 taatccccac atacgagtag aagatttgct gaaagaacca gcaacatatc cttgtaaccc
     6001 gtggatcact aatgaaaaaa ctgcccactg cggatatcct gaaatcaaat cgatcaatag
     6061 accactggaa gcaccaacaa ctaatccacc cgttggacca taaagaaaag cagcggtata
     6121 gatcccggcc tcgcataatg tcacaaatcc attcgtagcc ggtacaggga tcaaaattgt
     6181 taatgaaagg gcaactgtca acgcaatcag catagcatag atcgttaatt gcttagtttt
     6241 catttctcat tcctcctttt tgaataccgc cagaccagac acttccggta tgatcgttca
     6301 tcagcacgcc attagaaata caatgggcaa caaatttttt actttctgct actgctttgg
     6361 taaaatctgc ccctaacaca agattcgctg ttattgcaga agaaaaggca caaccggccc
     6421 cgtttactgt tgtagtgtcg atttttttat gagaaaatac ctcataatga tcaattgaat
     6481 agagaagatc cacagcttca tctccagcca tccttgtgcc gcctttgatc acaatgtttt
     6541 ttgttcctga agcagtgagt tctttagcga gatggatcat ttctgttaac gaagagggaa
     6601 gagcttgacc agaaagtaaa gcagcttctg ccaaattagg cgtaatcatc gtaacaagtg
     6661 gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt gatcacagct tcagtttctt
     6721 tgaatgccaa aacagggtct aaaatgattg ggatattttg ttttgttttt atgaagtctc
     6781 gaacaaccat gattgcttca cttgaattca ataacccaat tttgatacca tctaatgaag
     6841 tcattttatc aatcgaaact aactgtcgtt taagcaattc caaagatata gattccagca
     6901 taaatcgctc attaatatct agtgccccta tagaagtcaa ggcgcttagt ccgaatagac
     6961 caaaattctc aaaagttttt aaatctgttt ggattcctcc accagcaaat ggatcagaac
     7021 cgccaattgt caagattctt ttcataacat tttcctccat gaataagagt ataacgaaat
     7081 tttcaaaaaa gaaaaacagc caattggcat atttttatct catccaattt aaaaaaacaa
     7141 aaagaaaaga aagaccaaaa tggatctttc tttccttaaa tagttaggac ttttgtgatt
     7201 ttaattcttt ctcgatttga agccgttgca ctttcattgt agctgttcgc gggattgcat
     7261 caaaatcacg gatgataggg acgttcaagc gaggaagttc atggacttgc tcccaccatc
     7321 gattccaatc catttcttta tcaggagcta aagcaataat cggttgtgga gaatgatttt
     7381 tatcccttac aatcactact tctgccaaaa aatctaacga atctaataaa aagtcttcaa
     7441 tagctaatgt actattgatc gtttcgatca aatccacttg ccgatctttc aaaaataggt
     7501 ggccatgctt atccataaaa ccgtaatctc cgctatccca ccaatcgcca taaacatttt
     7561 cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg tcctttagac agtaattgta
     7621 tatgaccatc tgtgttttca ggtaggacat tcccaactga atctgtaatc cgagcgcttg
     7681 tcatgtcttc cagtccaacg cccatatctc ttgcatcact tgttttcaaa ctctctagtg
     7741 tatgagcctt aagaatcatt ggtccgcatt cactttggcc atatacttga agaaataccg
     7801 catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt ctggttgttt atagcatcaa
     7861 aagtggagtg atagtatcgg atacccgaaa aagcttctgg atgctcctta gcagtaaaac
     7921 gccactgaac aaagttattc ggatgggtct ctagtgcaat tggccggtga gcttccaaca
     7981 tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat cataggaaat cccatggaca
     8041 tcaaagaaga gataccaata ttgaatctgg aatgcactgg agagatgtga aaagcgatta
     8101 atttcttttc cgaaattttt gtgaaaactg ttttttgcca tttcgtccgc catcccatag
     8161 aatgagcaga atgacaaata agtttaggga taccagttgt accagaagta tgggtcatat
     8221 aagaaatttc atttttgtct aaaaatgatt gagaaacaga caaagcaggt tgttccatta
     8281 aataaagaat aggaatctgt tttgattttg ttccatttga aatttctctc actcgctttt
     8341 ttgtctcatc atcaaataaa atgaaagggt cttctaaacg atcgataaat acttccattg
     8401 ttgttgttgg tagatgataa gaagtcatga caggcactgc accaaggtaa gacacagaaa
     8461 cagctagaag gtatgtgtca aaagcggaac tcttataaat aataactttg tctccggatt
     8521 tcactccaag atgagccaat tgataagccc gtttcaacac actttcgcag cattttttgt
     8581 aagttgtttc aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag ataatcgggg
     8641 tctcggaaaa gttagctgca gcttcttgaa aattcgtaaa taagttcaaa ggggaatacg
     8701 ttatggtctc catcattcac actccattat cttttgttaa gtaatataca aattcaagat
     8761 aatcgtaaca taatccttta tataataaca tagtttattg tgaaaaaaag atcatacatt
     8821 tgttttatat ggaagaaacc cgaacgaatg ccgctaattc gtgataagaa aaatcaacca
     8881 tcttttccca gatatcttga aaagaatggg atgagaggta tcgattacca cataaataat
     8941 ttaccgcgtc agcatcatct aaatatagtt ctgcataata acgatatgtt tttctgcata
     9001 gttctggcga tacgttttta tatactgtaa ttttgctcaa aatttggcga atagtccgag
     9061 gagaaatttg ctgatcatgt gaggaacgaa aaacaaaaga attttcgtcc gtattttgtg
     9121 ctgttaactt aaaatagttc tccagaaatg gcatcgcttc ttttggtatt ggtactttat
     9181 tcagtctgtt ttcttttcta ttcagattaa gaatctgctt ggtcaaagat acatctttac
     9241 gtttaagttc tactacttca tggatcttta atccgcaata cagaagaatg gcagtaattg
     9301 caaggttcct cggtctattc ttttcttcaa agctagcttc tctgcttgtt tgaaagcctt
     9361 caatcgggat aaacaaatgc tcaaaatctt tgaaaagttc tgcatcgata tatcgcggag
     9421 ctgctttggt tgtttccggt aaacttgtac ccaaaagaga aatctcttct tcaatcagtc
     9481 ctttttgata caaaaatcgt aaaaaatgat ttaatgaata ttgcttcctt gaaatcccgt
     9541 aatagctatt ttcaaacaat tgctccgttt cttggttcgc tgtagtttgg aacgaacgag
     9601 tatgtcgata gattttagct aagaaattct gataatcttg tttttctaaa tggataatca
     9661 gctgatcatc cacttcttca gaaaatactt ttctcaaata atcagtaaac agatgaaaat
     9721 cttttgcgta ttcatagcga ctgcctaatg aaagggaaga acgattttct tctaaaaact
     9781 gttgaaagac ggaagggagc aagtgtgtac gttgctttaa cttttcttgc agttgttttc
     9841 tagttttgct gtccattaca aattccctcc aatagtgttc attttctata ttttaccgca
     9901 tttcatcaaa tgaagataga cagtttgtct tgatcttagg aagcaaattt ttaactaagt
     9961 actagctaaa agatatgaag taagaagata catttttagt tattttatta gtgcagtcat
    10021 tcttttgatc ttttaattga tttatcctct ttttagctat gtggttactt catgagcaat
    10081 gaaatggatt tggttcgttc taatggcagg aggctctttc ttttatttcc gaaaaattag
    10141 aaaacatttt ttcagaatcg attggctctc catttattca ataatggttt gtctaacgat
    10201 attgttggga taattcttaa agaagtatta gaaatagttg gtacttcatt actttacatg
    10261 aagatttatt ctatggtagg agcaattata ttgagaatta atcctttttt atataagttg
    10321 tttgctt
//
LOCUS       DAZULO010000093        10193 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000093 DAZULO010000000
VERSION     DAZULO010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.095"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..626
                     /locus_tag="ACG8OA_002335"
     CDS             120..626
                     /locus_tag="ACG8OA_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFS4977083.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            643..1542
                     /locus_tag="ACG8OA_002336"
     CDS             643..1542
                     /locus_tag="ACG8OA_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFS4977084.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1629..2513
                     /locus_tag="ACG8OA_002337"
     CDS             1629..2513
                     /locus_tag="ACG8OA_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS4977085.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2705..2776)
                     /locus_tag="ACG8OA_002338"
     tRNA            complement(2705..2776)
                     /locus_tag="ACG8OA_002338"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2741..2743),aa:Arg,seq:ccg)
     gene            2836..4170
                     /gene="rpoN"
                     /locus_tag="ACG8OA_002339"
     CDS             2836..4170
                     /gene="rpoN"
                     /locus_tag="ACG8OA_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFS4977086.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4319..5410
                     /locus_tag="ACG8OA_002340"
     CDS             4319..5410
                     /locus_tag="ACG8OA_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFS4977087.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5451..6452
                     /gene="gap"
                     /locus_tag="ACG8OA_002341"
     CDS             5451..6452
                     /gene="gap"
                     /locus_tag="ACG8OA_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS4977088.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6562..7752
                     /gene="pgk"
                     /locus_tag="ACG8OA_002342"
     CDS             6562..7752
                     /gene="pgk"
                     /locus_tag="ACG8OA_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFS4977089.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7856..8611
                     /gene="tpiA"
                     /locus_tag="ACG8OA_002343"
     CDS             7856..8611
                     /gene="tpiA"
                     /locus_tag="ACG8OA_002343"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFS4977090.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8727..10025
                     /gene="eno"
                     /locus_tag="ACG8OA_002344"
     CDS             8727..10025
                     /gene="eno"
                     /locus_tag="ACG8OA_002344"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFS4977091.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3565 a         1886 c         2112 g         2630 t
ORIGIN      
        1 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
       61 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
      121 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
      181 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
      241 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
      301 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
      361 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
      421 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
      481 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
      541 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
      601 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
      661 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
      721 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
      781 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
      841 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
      901 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
      961 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
     1021 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
     1081 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
     1141 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
     1201 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
     1261 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
     1321 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
     1381 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
     1441 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
     1501 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
     1561 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
     1621 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
     1681 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
     1741 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
     1801 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
     1861 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
     1921 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
     1981 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
     2041 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
     2101 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
     2161 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
     2221 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
     2281 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
     2341 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
     2401 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
     2461 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
     2521 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
     2581 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat tttctttggc
     2641 ggacagtttt cacttaaaaa taaagaagca actattcgta aattatgcta aaatagcagt
     2701 agaacgcgtc ctgagggaat cgaacccccg tctcaagaac cggaatctta cgtgatatcc
     2761 actacactaa ggacgcaagc actgggataa ttgtaactaa acagataaaa aattacaagg
     2821 ggattggaca aaaaaatgaa atttgagcaa aatttttcac aaaaacagca gcagacacag
     2881 aaactagcca tgacgcaaca gttgcaacag tccatccaag ttttacaata taacagtgag
     2941 gagctgttgg cattcgttga gaatcaagca atggaaaatc cattggtgga agttgttgag
     3001 ccggaatggc agccagatta cataaaagct tcttcatctt cttatgaagg agaagagaca
     3061 aattacctaa atcagattcc tgatacaaaa gggtctttat ttgattcatt gatcgaacaa
     3121 gtccatttga attaccgaga tacttttttg aggaaaatcg ttctttatct tgtagaatac
     3181 atcgatttga acggattttt gacgattagt ttagaagaag ccggaaaaga gattggagct
     3241 acgcctatcc aaatgctcga tgcgttgact ctgattcagc aattagaacc agctggagta
     3301 ggtgcacgta gtttgcaaga atgtctcatg cttcaaacag aacgagatga ttatgcgccg
     3361 gaattagctt atatcgtgtt ggaagaatgt tttgaggaac tggtggagcg aaaatggaaa
     3421 gaaatcgcac aacgtttttc tgttgatcta catgctgttc agcagatatt cgactattta
     3481 caaacgttga gtccaagccc tggccggatt ttcgaccgta gcagcgaatt gtacattatt
     3541 cctgatgtcc gggtattagt ggacgacgat aaaaatgtac aagttatctc aaaccgaaaa
     3601 aatcagccaa atatacgctt tcaagagagt tacttcaaac aaatgtcaca acaagcagat
     3661 aaagaaactg aaaattattt aaaagaacgg aaacaggaat tcgaatggtt gaagaaaacg
     3721 atcctacaac gtggggatac aattttgcgg gttgctcaag tcattgtttc ccggcaaaaa
     3781 gctttcttta tcgacaagga acgtccaatc aagcctctta cgttaaagga agttgcaact
     3841 gagatagatg tgcatgaatc cacggtcagt cgtgcagtca acgggaaata tttggaaaca
     3901 gacttcggtg tatttgaatt aaaaaaattt ttcactacac gaattcctac gaatagtaca
     3961 gaacagacag aagacctttc agcagataca gcgaagaaaa agctgcagga attagtcgat
     4021 caagaagaca aaaacaagcc gttgtctgac cagaaattgg ttgaattgct gaaaaaagat
     4081 gagatagcga tctcaagacg aacagtagca aaatatcgtg atttattagg aatcccgagt
     4141 tcatcaaaaa gaaaacgtta tgataactaa tcagtcaaaa gactagaaaa agtatttcac
     4201 tttttctagt ctttttagtt gaaaaacgag agcaaaactg ttagacttac agtcgtgagg
     4261 ttgagatttc cctttattga tgtgaaattc gaatagttaa tttctttttt tatcgtatat
     4321 gggtcgtttt aagtctacat tggacgtaaa gtgtccaact aaggagatcg ctatgctgga
     4381 tgaaataaaa atgattgaat ctgttgcacc agatattatc gaagtgttgc aagaacgcta
     4441 taaaatttta cgaaatatct attggatgca gccgattgga cgtcgaagtt tatcggaaag
     4501 tatggggatc acagaacgag tattgcgaac agaaacagat cttttgaaaa atctgaattt
     4561 gatcgacacc tcaaaaagcg gtatgacgct gacaactaag ggcgaagaag tttatcaaag
     4621 ccttgaaaac ttcatggatc agcttttagg tacgcatcaa accgaacaac agttggcaga
     4681 atattttggt aaccaacgct gtatcgttgt ttcaggaaac agcgaagagc agaccaaagt
     4741 ggctgatgca ttcggcgaag cgctgagcga agccctagac cgactacttc cagaaggcga
     4801 gaatatcatc gctgtgatgg gaggaactac tatggctgtg gtagccgaac aattgtctaa
     4861 tctggaaaat aagaaacggc ataacctgtt tgtccctgca agaggcggga ttggtgaagc
     4921 gataactgta caggcaaatt ctgtcagtgc aagaatggct gctaaagcaa acggtaacca
     4981 ccgtgcactt tatgttccgg aacaattaag tctggcaact tacaattctt tactcaatga
     5041 gccatctatc caagaagtct taaacttgat tagtgaagcg aattgtgtta ttcatagtat
     5101 tgggcgtgca ttacacatgg cagcacgtcg taagatgact gaaaaagaat tagtcatgct
     5161 gaaacaagca aatgctgttg cggaatcttt tggctacttt ttcaatgaaa aaggtgaagt
     5221 cgtttataag gtccctagaa tcggcataga attgcaagat ttggaaaaag tcccgatcat
     5281 catggcaatc gcgggcggaa aatcaaaagc caaagcaatt cgagcttata tgaaaaatgc
     5341 accaaaacaa acgtggctca tcactgatga agctgctgca aatgagattt taaaaggggt
     5401 aaccctttaa aataaatatt tttttgattt tcataaggag gaaatcttaa atgacagtta
     5461 aagtaggtat taatggtttt ggacgtattg gacgtctagc attccgtcgt atccaagatg
     5521 tagatggaat cgaagtagta gcaatcaacg acttgacaga tgctaaaatg ttggcacact
     5581 tgttaaaata cgacacaact caaggacgtt tcaacggaac agttgaagtt catgaaggtt
     5641 cattcaacgt aaacggaaaa gaagttaaag tattagctaa ccgcaaccca gaagaattac
     5701 catggggcga actaggcgta gatatcgttc tagaatgtac tggtttcttc acttctaaat
     5761 cagcagctga aaaacattta acagctggtg ctaaacgcgt tgttatctct gctcctggcg
     5821 gaaacgatgt tccaacaatc gtttataaca caaaccacga aacattaact ggtaaagaaa
     5881 cagttatctc aggtgcttca tgtactacta actgtttagc tcctatggct aaaacattga
     5941 acgacaaatt tggtgttgtt gaaggattaa tgacaactat ccacgcttac acaggtgacc
     6001 aaatgactct agacggacct catcctaaag gtgacttccg ccgtgcacgc gctgctgctg
     6061 caaacatcgt tcctaactca acaggtgctg ctaaagctat cggtttagta attcctgaat
     6121 tgaacggtaa attagacgga gctgctcaac gtgttcctgt accaacaggt tcattaacag
     6181 aattagtaac agttcttgaa aaagaagtaa ctgttgacga aatcaatgca gcaatgaaag
     6241 aagcttcaaa cgaatcttac ggatacaaca cagacgaaat cgtttcttca gatatcgttg
     6301 gtatgactta cggttcatta tttgatgcta cacaaactaa agtaatgaca gttggcgaca
     6361 aacaattagt taaaactgtt gcttggtacg acaacgaaat gtcatacact gcacaattag
     6421 tacgtacttt agaatacttc gctaacttat aagatagaca aacgtacaag tgtaaattat
     6481 agacatgtac atttaaaagc ggggaagcaa tcgcgcttct tccgcttttt ttgataataa
     6541 gtttattcag gaggaaataa aatggctaag aaaacagtga aagatatcga tttaaaagac
     6601 aaaaaagttc ttgtccgtgt tgacttcaac gttcctttga aagacggcgt gatcactgac
     6661 gacactcgta tcaaagcagc attgccaaca atcaactacg ttctagaaca aggcggaaaa
     6721 gcaatccttt tctctcacct tggacgtgta aaaacagaag aagataaaga aggaaaatct
     6781 ttagctccag ttgcaaaacg tctaggcgaa ttattaggta aagaagtaac attcgtacct
     6841 gaaactcgcg gagaacaatt agaagaagca atccgcaaca tgaatgacgg cgacgttgtc
     6901 gtattcgaaa atacacgatt tgaagatatt gacggtaaaa aagaaagcaa aaatgataca
     6961 gaactaggta aatactgggc ttcattaggc gatgtatttg ttaacgatgc tttcggtaca
     7021 gctcaccgtg cacatgcttc taacgtaggt atcgcttcaa ctggtatccc aacagttgct
     7081 ggattcttga tggaaaaaga aatcaagttc atcggtgaag cagtagaaga accaaaacga
     7141 ccaatgattg caatccttgg tggcgcaaaa gtttctgata aaatcggcgt aatcgaaaac
     7201 ttgattccta aagcagataa aatcttgatc ggtggcggaa tgacttacac attctacgaa
     7261 gcaaaaggaa tcaagatcgg taactctcta gtagaagctg ataaagtgga actagcaaaa
     7321 gaattaatcg aaaaagcagg cgacaaactt gtcttgccaa tcgacaatgt ctgtgcacca
     7381 gaattttcaa atgatgttga aacacaagta atcgaaggag acattcctga tggtttgatg
     7441 gctttagaca tcggcccagc atcagtaaaa ttatttgctg atacattaca aggtgcaaaa
     7501 acagttgttt ggaacggacc tatgggtgta tttgaaatga gtaactttgc taaaggtaca
     7561 atcggtgttt gtgaagcaat cgctaactta gaaggcgcaa caacaatcat cggtggcgga
     7621 gactcagccg cagcagcaga acaattagga tttgctgaca aattcacaca catctcaaca
     7681 ggtggcggcg caagcttgga attgcttgaa ggtaaagaat taccaggact agcagcaatc
     7741 aacgataaat aaacgaccaa gtttgctcaa ttctgacgag taatcgatca aaaggtgaag
     7801 ggagttttca tgactcccta gccatttttc aataaattaa ggagtgtgtt cctcaatgcg
     7861 taaacctatt atcgcaggta actggaaaat gaacaaaact gcatcagaag caaaagaatt
     7921 tgctgaagca gtaaaaaaca aaatcccttc aaatgacaaa gttgattcag tcatcggatc
     7981 tcctgcatta ttcttacaag aattaatgac atcagctgaa ggtacagaac taaaaatctc
     8041 agcacaaaac tgctactggg aaaactcagg tgcgttcact ggtgaaactt caccagcagc
     8101 tcttgctgat ctaggtgttc attatgtgat catcggacat tcagaacgtc gtgaatattt
     8161 ccatgaaaca gacgaagaca tcaataaaaa agcgaaagct atctttgcaa atggtatgac
     8221 accaatccta tgctgtggcg aatctttaga aacctatgaa gcaggcaaaa ctgctgaatg
     8281 gatcgaagga caaattacag ctggtttagc tgatctttca gctgaacaag tgtcaaacat
     8341 ggttattgct tatgaaccaa tctgggcaat cggtactggt aaatcagctg acgcacaaat
     8401 cgctgacgaa atttgtggag ttgtacgtca aacagttgaa aaactttatg gaaaagaagt
     8461 atcagaagca gttcgtatcc aatacggcgg ttctgtaaaa ccagaaaata ttgctgaata
     8521 catggcaaaa gaaaatgttg acggtgcttt agtcggtggc gcaagcttgc aagcagattc
     8581 attcttgtca ttgttagacg ctgtaaaata attgttgaaa atttgcgaaa aatgcaagaa
     8641 atgtttgcac taatgagcaa aattaactaa aatcgtatat gagggaaatc ccttaggaaa
     8701 aaacaaactc aaaggagaga caaaacatgt caattattac tgatgtttac gctcgcgaga
     8761 tcttagactc acgtggtaac ccaacaatcg aagtagaagt atacacagaa agcggagctt
     8821 ttggccgcgg aatggttcct tcaggtgctt caactggtga atacgaagca gttgaattac
     8881 gtgacggaga caaagctcgt tatggcggta aaggtgtaac taaagctgtc gacaacgtaa
     8941 acaacatcat tgctgaagca atcattggct acgatgttcg tgaccaaatg gctatcgaca
     9001 aagctatgat cgctttagac ggaactccta acaaaggtaa attaggtgcg aacgctattt
     9061 taggtgtttc tatcgctgtt gcacgtgcag ctgctgacta cctagaagta cctttatacc
     9121 actacctagg cggattcaac acaaaagtat tgccaacacc aatgatgaac atcatcaacg
     9181 gcggatcaca tgctgataac tcaatcgact tccaagaatt catgatcatg cctgttggcg
     9241 ctccaacatt caaagaagct ctacgtatgg gtgctgaagt attccacgca ttagcttcaa
     9301 tcttgaaagc tcgcggatta gcaacttctg ttggtgacga aggtggattt gctccaaacc
     9361 ttggttcaaa cgaagaaggt tttgaagtaa tcatcgaagc aatcgaaaaa gctggctatg
     9421 tacctggtaa agacgttgtt cttgctatgg atgctgcttc ttcagaattc tacgacaaag
     9481 aaaaaggtgt ttacgtacta gctgattcag gcgaaggcga aaaaacaact gacgaaatga
     9541 tcaaattcta cgaagaatta gtttctaaat atccaatcat ctcaatcgaa gacggattag
     9601 acgaaaacga ctgggacgga ttcaagaaat taactgacgt attaggcgac aaagttcaat
     9661 tagttggtga cgacttgttc gtaacaaaca ctcaaaaatt atctgaaggt atcgaaaaag
     9721 gaattgctaa ctcaatcctt atcaaagtga accaaatcgg tacattaaca gaaactttcg
     9781 aagctatcga aatggctaaa gaagctggct acacagcagt tgtatctcac cgttctggtg
     9841 aaacagaaga ttcaacaatc tctgatatcg ctgttgcaac aaacgctggc caaatcaaaa
     9901 ctggttctct atcacgtact gaccgtattg ctaaatacaa ccaattacta cgtatcgaag
     9961 accaacttgg tgaagttgca gaatacaaag gtttgaaatc tttctacaac ttaaaaaaca
    10021 aataattctt ttctaatgaa gatacatgat cgactattga aagatagtcg agtatgatca
    10081 cttagttaag taatcgaact ctctacttgc tgaaatatcg gctctttgtc aataaggact
    10141 gatgagttgt gcaaaatcaa atctgagtca gaatgaacca cattctggct cag
//
LOCUS       DAZULO010000094        10086 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000094 DAZULO010000000
VERSION     DAZULO010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.096"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(247..759)
                     /locus_tag="ACG8OA_002345"
     CDS             complement(247..759)
                     /locus_tag="ACG8OA_002345"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFS4977092.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="ACG8OA_002346"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="ACG8OA_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFS4977093.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3655)
                     /locus_tag="ACG8OA_002347"
     CDS             complement(3212..3655)
                     /locus_tag="ACG8OA_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFS4977094.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="ACG8OA_002348"
     CDS             complement(3656..4444)
                     /locus_tag="ACG8OA_002348"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4977095.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /gene="rnz"
                     /locus_tag="ACG8OA_002349"
     CDS             complement(4441..5382)
                     /gene="rnz"
                     /locus_tag="ACG8OA_002349"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFS4977096.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="ACG8OA_002350"
     CDS             complement(5559..6146)
                     /locus_tag="ACG8OA_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFS4977097.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="ACG8OA_002351"
     CDS             complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="ACG8OA_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFS4977098.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="ACG8OA_002352"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="ACG8OA_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS4977099.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="ACG8OA_002353"
     CDS             complement(9063..9998)
                     /locus_tag="ACG8OA_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4977100.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DAZULO010000095        10084 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000095 DAZULO010000000
VERSION     DAZULO010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.097"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            162..392
                     /locus_tag="ACG8OA_002354"
     CDS             162..392
                     /locus_tag="ACG8OA_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFS4977101.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="ACG8OA_002355"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="ACG8OA_002355"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS4977102.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="ACG8OA_002356"
     CDS             1803..1979
                     /locus_tag="ACG8OA_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS4977103.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="ACG8OA_002357"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="ACG8OA_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFS4977104.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="ACG8OA_002358"
     CDS             2474..2941
                     /locus_tag="ACG8OA_002358"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4977105.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="ACG8OA_002359"
     CDS             3026..3910
                     /locus_tag="ACG8OA_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4977106.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4024..4260
                     /locus_tag="ACG8OA_002360"
     CDS             4024..4260
                     /locus_tag="ACG8OA_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFS4977107.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="ACG8OA_002361"
     tRNA            4348..4428
                     /locus_tag="ACG8OA_002361"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="ACG8OA_002362"
     tRNA            4435..4506
                     /locus_tag="ACG8OA_002362"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            4631..4801
                     /locus_tag="ACG8OA_002363"
     CDS             4631..4801
                     /locus_tag="ACG8OA_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977108.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5021..5728
                     /locus_tag="ACG8OA_002364"
     CDS             5021..5728
                     /locus_tag="ACG8OA_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4977109.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="ACG8OA_002365"
     CDS             complement(5788..6432)
                     /locus_tag="ACG8OA_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFS4977110.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="ACG8OA_002366"
     CDS             6559..6846
                     /locus_tag="ACG8OA_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977111.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="ACG8OA_002367"
     CDS             6954..7274
                     /locus_tag="ACG8OA_002367"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4977112.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="ACG8OA_002368"
     CDS             7355..8668
                     /locus_tag="ACG8OA_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS4977113.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="ACG8OA_002369"
     CDS             8665..9384
                     /locus_tag="ACG8OA_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFS4977114.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="ACG8OA_002370"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="ACG8OA_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFS4977115.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3359 a         1668 c         2151 g         2906 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaa
//
LOCUS       DAZULO010000096        10024 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000096 DAZULO010000000
VERSION     DAZULO010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.098"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(488..712)
                     /locus_tag="ACG8OA_002371"
     CDS             complement(488..712)
                     /locus_tag="ACG8OA_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977116.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            903..3680
                     /locus_tag="ACG8OA_002372"
     CDS             903..3680
                     /locus_tag="ACG8OA_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4977117.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3761..4180
                     /locus_tag="ACG8OA_002373"
     CDS             3761..4180
                     /locus_tag="ACG8OA_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4977118.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4192..4662
                     /locus_tag="ACG8OA_002374"
     CDS             4192..4662
                     /locus_tag="ACG8OA_002374"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4977119.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4679..5458
                     /locus_tag="ACG8OA_002375"
     CDS             4679..5458
                     /locus_tag="ACG8OA_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4977120.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5445..6266
                     /locus_tag="ACG8OA_002376"
     CDS             5445..6266
                     /locus_tag="ACG8OA_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4977121.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6283..7332
                     /locus_tag="ACG8OA_002377"
     CDS             6283..7332
                     /locus_tag="ACG8OA_002377"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4977122.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7345..8349
                     /locus_tag="ACG8OA_002378"
     CDS             7345..8349
                     /locus_tag="ACG8OA_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4977123.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8982..10001
                     /locus_tag="ACG8OA_002379"
     CDS             8982..10001
                     /locus_tag="ACG8OA_002379"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS4977124.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3362 a         1651 c         2094 g         2917 t
ORIGIN      
        1 tttaatttgt tcttgcaatc ttttttgcta cgtaattttt gtaaaaaagt gggttgagag
       61 aaatctaatt gattttcaaa gaaggagtat gaaaatgaga aaaatattta ttcgtttatt
      121 gtgggtacta tcttagagga ataaataata gtggagaaaa ctatggtttg gcgcagattc
      181 tatcaggttt gatggaacga agacaacctc ccgacatcaa gtcaaaagct acttgagatt
      241 ttgatctgaa aataaaaaaa gaaaacaagg taggaacatc aatgtttcta ccttgttttc
      301 gtaatcaatg ggaaaaaact aagagtggtg tacgtaatca ataaaaagga tggtgacaac
      361 agtacctctt tactctctga aagcatcata actcgttttt cctcattatg ccaataaatt
      421 gttgttatac cacatataag gatttgtaat agctaaaaac agacaggaaa gttgaaatct
      481 gtgaatctta ttttgtatgg aaggatttat agaacgcaaa aatcaagata aagatgaata
      541 aaaatcctgt ttcggctata tattgcgtat ctacgccaat aaaacgagtt tgttctgtca
      601 tagccatata aagcaaaggc aaaaatacaa ctacagcgag ataagatccg atgatttctc
      661 gaaaagtttt ccaggaagta tgtttcctgt ttttatattt atccgatttc attgaatagt
      721 catagcctcc ttattctttc atatgttgaa attccagtat aaaaactgga aggaactgta
      781 cgttttttag aataaaaatt ataagggata agtaggctgc tgtctataat aagatatatt
      841 tttcaattct ttattaaaga ttttattgac aaaatgaatc cgttttctat gatggaggag
      901 gggtgaataa gttgttgaag aaggaaattt tatgttactt agagaaccag acagcctttt
      961 tcgatttaga acatatgaac gaggtcttta cagcacaaag gttagctgat cattttgggg
     1021 taaaaagaaa cacagtcagt cattatttga accagttaaa tgatgagcaa ctactagtca
     1081 aaatcaattc tcgaccagtg atttatttcc acaaagaagc ttttgaaaaa caatttttta
     1141 agttacgcac gaatttttat ttttctatcg atgatctcaa agaggaacag ccttttttcg
     1201 cgcaaccaaa agacttattc tcttttatga tcggatacga cgccagtttg aaagaaagta
     1261 tcgaacaaat caaaacagct ctttactatc cagatggcgg attaccactt ttgattacag
     1321 gcgaaagtgg cacaggtaaa agttatttag ttaatttggt ctatcagtat tgtctgcttc
     1381 atgatctgct agaagattct gcaccattca tcacttttaa ttgtgcacaa tatgcagata
     1441 atcctgaact tttgactagc aatttgtttg gacatgttaa gggagcgtac actggtgcag
     1501 aagaaaataa aaaaggggct tttgaagcag cagatggtgg gatcttgttt ttagacgagg
     1561 tacatcgctt aacaccagaa ggtcaggaaa aactgttcac ctatcttgat caaggtgtga
     1621 tttatcgtat gggagaacct aatttgtcac gccggatcaa aacacgtctc ttttttgcta
     1681 cgactgaaga gattactagt catttcttaa caactttcat tcgaagaata cctatccgaa
     1741 tcgaactacc tgcacttaat cagcgtagtc gtaatgaacg gctagaactg atttactctt
     1801 tttttctcga agaacaaaga aaaatgggga gccctttgtc agtcagtgga ccagctttgt
     1861 cacttttaac aagtcagaaa ttatccggaa acataggaga gctgaaaaat atcgtcaaag
     1921 tttcagcagc aaaagcatat gctgaacagc gtgaacagcc tgaaatccat gtcacgatcc
     1981 atcatttgca aaaagaacta cttgcgcagc ctattactaa aaatacagca cagcaaggaa
     2041 tctatattac gcaagaccag accttagagc aattaatgga gaagcaacag cccgaacagc
     2101 agcgaatcgt taaaagtttt gagcaaatcc taattgattt caagaaaaat aactgtctgc
     2161 tatattcttg cgaaggaaaa ttaaagcaag aagtgattca attatttgat tttttgctgt
     2221 ttgagaccag tagacaagaa aaacacgagc tgctggttta ttggacacaa tctatccgag
     2281 agacctttcg acaaatggaa actgcctatc aaatcaaatt cgatggaaac agtgtctatg
     2341 ccttgagtta ctatctctat cagcgaagca ctatcaaatg gttaccggaa gaacatgata
     2401 ttatccaact aatcaaagaa ttagaacaac aagtttctca gtcttatcca tctagttatc
     2461 actatgtcca acgaattctg gaattaagta agatcaaatt agatattgaa gacacaagta
     2521 tggatcggat tttactgacg atctatctaa aaaaagcgaa atggtcaaaa gaaaaacgac
     2581 tgccaaaagc gatgattgct gctcacggtt atgcaactgc tagcagtatg gcaaatgtag
     2641 taaatcgttt attacaagag gatctatttg aatcatttga tatgccacta gatgtcacac
     2701 cacagcagat tgcttcagaa atccttgatt attgcgaaaa cagcgatgtt tcaaatggat
     2761 tgatcatatt agtagacatg ggatctctga aagaaatcca tcagtttttt aaaaaacagt
     2821 tatccgtacc gcttttgatt ttgaataatg tgacgacacc tttagcaatc acagtagggg
     2881 aatgcctgca aaaaaatgta tcattagaag agatagcaga agagactgtt cgacagatcc
     2941 agccagaatg gcgactgctc tatccagagg aaaacaaacc caaagcactc attactactt
     3001 gcttcactgg aatcggtacg gcggtccact taagtgaact gctagaaaaa agcttaccta
     3061 cggcttgtca gttgaaaatc atcccatatg aatatcagca attaaaagat aagaaaaaca
     3121 gtgacccatt attttccata tacgaagtcg taggaatgat tggcaccaca gatcctgata
     3181 tcaccaatat cgactatctt tcattagaag acctcatctc gggggaaaag atgtctgtac
     3241 tagctgaatg gttggacact actatgaatg cttcagagaa ggaaatattc aatcagcgca
     3301 tcattcgtaa tttttcattg gaaaaagtac tggattccgt gactattcta gatacagaga
     3361 aggtgatggg agaaatcgat cattttatga gagaattaga ggtgcagttg aatcgaagca
     3421 tcagcaatcc caaaaaatta gcgttgtatg tccatgtgag ctgtctcatt gaacgattga
     3481 tccgacaaat gccaatcgaa acctatcaag gattagagaa atggaagcag tgtcaaaaag
     3541 aagggttatc tgctattaaa tctgcgttta gtgtcattga agagcattat agtgtcatga
     3601 tccccaattc tgagattgcc tatatctatg atattttgag tgaagataca gagtttctct
     3661 cgatagaaga agaattttga acataagcaa gttgttgcca ctaattttgg catgcaactt
     3721 gcttttttag tatgtgtaag tgaaaagagg agatgaaaaa gtggatagag aaattatctt
     3781 agcttcccat ggaaaatttg cttcagggat attagattca ttagagctga tctatgggaa
     3841 aaatcattct attactgtat tcgattgcta tactgatgag aattttgatt tagcggaaac
     3901 agtaaggcag ctttttatga ggtacaaaga taaagaaatc attgttctga cggatctttt
     3961 tggcggaagt gtcaacaacg aattcttaca atatatcaat cgggatcatg tctatctcgt
     4021 tgccggattg aatttgccac tattgattga gctagtatct cggttggacg ctgaagtaga
     4081 gacaactgtt ttgatccagc aggtgctaac tgattcaaaa aaaatgatcc agttttgtaa
     4141 cgagcggctc aatcagaaaa tagaagaaga cgaattttag gaggaagaaa aatgattgta
     4201 ttgacaagag tcgaccatcg gttattacac ggacaagtag ctttttcatg gacacaaaca
     4261 ataggcgcag actgtatttt gattgctaat gatgatgtac ctacgaatga gatacgaaag
     4321 acaacgatca aattagccaa gccacaagga gtcaaactag tgatcaaatc aattgatgac
     4381 tcgattgccg cgttgaaaag cggggtgaca gataaatata agttattcat cgtagtggaa
     4441 tcgatcgagg atgcatataa gctggcagaa gcgtatccag aaatcaaaac catcaactta
     4501 ggagggatca aggcaaaacc tggtactcgc agcatcggaa aagcggtaaa tattttacca
     4561 gaagaggagg aacgcttgaa acgattaatt gacaaacaaa tagaagtaga aatccgtcaa
     4621 gtaccagcag ataaaaaagt ggatgtaaga gaagttttgt aagaaaggaa gaacctcaat
     4681 gattgtacaa gcagtcttat taggaattgt tgcatttgtc gcacaatcag aatatgcact
     4741 agggacatcg ttgctttctc gtccgattgt gacggggctg tttacaggaa tcgtgttagg
     4801 tgatgtaaaa actgggatca ttatgggcgc aactttagag ttagcattta tcggatcgtt
     4861 ctcagtaggt gcttcgattc cacctgatgt tgtgaccggt gggattttag ggacggcttt
     4921 tgctattaca gctggagccg gtacagaaac cgcgctttta ctaggtttgc cgattgctac
     4981 gttgacattg atattgaaaa atatttactt agggcttttg attccaatta tgaaccataa
     5041 agcagacaat tatgcagaag aaggaaatta taaaggagtc gagcatatgc acttgcttgc
     5101 cggtatcggt ctttccttga tgcttggtgt tgtggtaatg gtttcttata tggtgggaag
     5161 caatacgatc ggaaatctat taaatatgat tcctgacttc gttcaaaagg ggttgtcagt
     5221 agctacaggg ttgattccag ctcttggttt tgcgatgctt gctcgtttgc tgatcaacaa
     5281 acaagttgca ccatattttt tcttaggttt tgctatagct tcttacttag agattcctgt
     5341 gaccggagta gctatttttg gtgcgatttt agcagttgtg gtagtcaata ttatgaatgt
     5401 ccgtcaacta ccacttcaaa caactgcagg ggaggtagaa gaagatgaag aattctgaga
     5461 tcaaaaacag caaaataacg aaaaaagagc taaatcacgt attttggcgt tcttttcaaa
     5521 tggagttttc ttggaattat gaacgtcaga tgaatatggc ttatgtttat gcaatgattc
     5581 cgattttgaa aaagttatat cagtcgaaag aagaaatggc agcagctttg aaacggcatc
     5641 tagagttttt taacacgacc ccacatatcg ttacactgat tttaggaatc acggctgcaa
     5701 tggaggaaga aaataaggat gatcctgaat ttgatgtgac tgcgatcgat agtatcaaaa
     5761 catctttgat gggacctctt gcagggttag gagattcttt cttttgggga actttgcgat
     5821 tgattgccac aggtgtgggt acctctttag ccatgcaagg aaatatttta ggccctatct
     5881 tgttcatttt gattttcaat attccgcaca ttctgtttcg ctatctagca acaggttggg
     5941 gatatcgctt aggaacgggt tttttgaaaa agatccaggc aaacggtatg atggaaagtt
     6001 tgacattagg cgcgtctatt attggactga tggtcgttgg agcaatgact gccacgatga
     6061 tcaccatcaa tattccttta aaaatcggtt caggagaaaa tgctgtgact gttcaaagta
     6121 tttttgatga tatcgttcca aatatcttat gtcttggagc atttggcata gtgttttatc
     6181 tattgaagaa agaagtgaaa ccattaacga tcttgatcgg actagctttc tttggaattt
     6241 ttggttcgtg gatagggatt ttttaaaaaa ggagcagaga aaatgtttac gatgcaagat
     6301 tatatctatg aagaaaagga agtactaagt acgatcttga aaaagaatga tttttcgaca
     6361 agagaacata tgaaaaaaac agtgaattta ctgatcttag caacgggttc atcttataac
     6421 gcttgtttag cagcgaagcc agcattggaa tcttatggtg atcttactgt cgatattcaa
     6481 gaaccttttt attttttcaa ttatggaaaa ctttctcctt caatcgatac tgtgattgct
     6541 gtgtcacaaa gcgggaaaag tgcttctact gttgaagcag taaaaatgat acaaaaacaa
     6601 gggttacctg ttgttgctat cacaaatgac gtgcaaagtc cacttgcttt agaagctgca
     6661 caaattattg atttaggtgc cggggtagaa tcagtaggct tcgttacaaa aggctattct
     6721 gcgactgttt tacagttact actcttaggg ataggaattg gaataagtaa aaataaaatc
     6781 agcaaaaaaa tagaacaaga ctacatgcaa cagttgcgca aaataatcaa ccatttacca
     6841 gcgattattc aaaaaacgga agaatttttt gatggatatc aaagtttatt tcgtttagca
     6901 cagcgtttcg tcaccattgg atatggtcca aactggggaa cagctaaaga agcagagacg
     6961 aaattgactg aaacaatccg cgttccgtca caaggatttg agttagaagc ttatatgcac
     7021 ggcccatacc tggaggcaga tgcttcgcat ttgctattct ttattgaagg ggaaagtgta
     7081 aataaggaac gttcgcaaaa gttgcaaaga tatatgagtc ggtacgttgg agagactctc
     7141 accattacca caaaaaaagc aagaaacgag aaaacacttg gattagcaat agaatgtgat
     7201 gaatatctgt ctgtattagc gcttgtcgtt ccgtttcaac tattcgctta taaaaccgca
     7261 gtagctaaag gtattgactt gaacaaaaag atttttgaag actttgatac agtcttgaaa
     7321 agtaaattat aagaggtgga aatcatgctg aaattcaatg aagaagaaca aatcaaagca
     7381 ataaaaggcg cactagcttt acgtccgcaa gtagaagaaa tcatcgataa actttatgcg
     7441 gaaaaatttg acgctgtcta ttatctagga atcggtggaa cgtatgcttc ctcgatgcaa
     7501 gctgtcactt atatgaatgg gaaaagcaac ttgcctgtct ttgtacagca tgctgcagaa
     7561 tattacacta ctggaaacaa aagattaaca aaagattcta tcgttatttt gtcctctgtt
     7621 accggtacaa ctcaagaagt agtgaaagca gtagaacaaa tcaagaaagt aggagcgaca
     7681 ctgattggat ttatcgataa agcgaacagt aagttgtctc agctatgcga ttttgtggtc
     7741 acttatccag ctcctggaac agaacaaatc aaatttttta tggcagcaga tcgattgatg
     7801 tatttgaatg gagaattcga agcttattca gaatactatg aacagctcga acgttattta
     7861 ccaacagggt tagtggaagc agagaaaaaa gcagatgcat ttggcttatc atttgcagaa
     7921 aaacatcgac gtgatagtat gcattatttt attggagcag ggaatcaatg gggagctgtt
     7981 tattcatatg ccatgtgtta ctgggaagaa caaagctggc ttccatcgaa atctatccat
     8041 gctgccgagt ttcttcacgg gacgttagag atcgtagaag aaacaacccc agtcacatta
     8101 tttttaggcg aagatgaaca gagagtgtta tctgaacgtg tggctaaact tttgccaaaa
     8161 atttgcagca attatacatt gatagacaca aaagactatc cagtagaagg tatcagtgaa
     8221 aaatatcgcg gacgtgtttt gtctttctta ttgatgcatg tagtgacaca acgcatcgat
     8281 gctcatgtgg agcagttgaa ctgccatcca ttagagatac gccgctacta tcgtcagttt
     8341 gattattagg atagactgga taaaaagaat ataaaaaata aagagagcag cgataactgc
     8401 tccctttatt tttttaaaat attggcatca attacgttat aaaacacatt ttttgcatta
     8461 tctacagttc aacttataat ctgtttaatg ggacatgtgg gtgccaacca ttttttgtta
     8521 tgcaatagta ctatttagtt gttgcatgga tccgtagtaa aagtccaagt acaacgagtt
     8581 gcgccagttt acatagcgtt ttgtatccag aatgttgact aaaacaaaag ttcctatgtc
     8641 aaccgtcaac agagacaatc tgtccgttgc aggacctgac agatttagaa atgttcaatc
     8701 tgtctcttat gtattttaaa tcaatactca ctaggagaag tttcctaacc tcctaatagt
     8761 aatgctggta gtaacattgt caaaactgct tcctcttagg aacaagcaaa gcataacgag
     8821 tgtaaaaata aaatgtagac tattgtaaca tatcattgat tatttcaagt aaaaacgata
     8881 aaagaagtaa acaaacactt aaaaacaatg gaaattagtc ccaactttaa gaaatgtata
     8941 ctataatgga aattatcaat tatttatttg gaggaatatc catgaaacaa aaaatcgcag
     9001 ttcttggtcc tggatcttgg ggcactgctt tagcacaaac attggcagaa aacgggcatg
     9061 atgtccgtat ttggggaaat gtacctgaac agattgatga aatcaataag tatcatacaa
     9121 atcagcattt tttacctgat ttgacgatcc cagaatcgat tatcggatat aaagaattgg
     9181 cagaagcagt ggataacgca gatgctattt tatttgttgt tccaacaaaa gcgattcgtt
     9241 cagtcgcaca ggaacttatt ggtaaaatga atactaagcc aatcattata catgcaagta
     9301 aaggactaga acaagatacg cataaacgaa tctcagaagt tttggctgaa gaaattccag
     9361 aagaaaaaag acaggcaatt gttgtattat ctggtcctag ccatgctgag gaagtggcag
     9421 tacatgatat cacaacaatt acagctgcaa gtattgatca aaaagcagca gcctatgtac
     9481 aaaaactgtt catgaatgat tatttcagga tttatacaaa taatgatgtg attggcgtag
     9541 aaactggtgc agcactgaaa aatatcattg caattggtgc cggcgctatc catggattag
     9601 gattcggaga tgatgcaaaa gcagcaatca tgactcgagg attggcagaa atcagccgtc
     9661 ttggtgtcgc gatgggtgcc aatcctctga catttatcgg tttgagtggt gtgggggact
     9721 tgatcgttac ttgtacaagt gttcattctc gcaattggcg tgccggtaac ttgttaggac
     9781 agggacataa gctggaagaa gttctagaaa acatgggaat ggtcgttgag ggtgtggcaa
     9841 cgacaaaagc agcagtagag ctagctcaac agctaggtgt tgagatgcca attactcaaa
     9901 caatctataa tgttttgtat aatggagaag atatcaaaaa agcagcaaaa gaaatcatgt
     9961 tacgtgacgg aaaaatggag aatgaatttt ctcttcgtta aatagatgat caatagttaa
    10021 tcgt
//
LOCUS       DAZULO010000097         9830 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000097 DAZULO010000000
VERSION     DAZULO010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.099"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..205)
                     /locus_tag="ACG8OA_002380"
     CDS             complement(<1..205)
                     /locus_tag="ACG8OA_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977125.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(221..835)
                     /locus_tag="ACG8OA_002381"
     CDS             complement(221..835)
                     /locus_tag="ACG8OA_002381"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFS4977126.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(982..1665)
                     /locus_tag="ACG8OA_002382"
     CDS             complement(982..1665)
                     /locus_tag="ACG8OA_002382"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS4977127.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1774..2232)
                     /locus_tag="ACG8OA_002383"
     CDS             complement(1774..2232)
                     /locus_tag="ACG8OA_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFS4977128.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2316..4079)
                     /locus_tag="ACG8OA_002384"
     CDS             complement(2316..4079)
                     /locus_tag="ACG8OA_002384"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFS4977129.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4300..5760)
                     /locus_tag="ACG8OA_002385"
     CDS             complement(4300..5760)
                     /locus_tag="ACG8OA_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977130.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="ACG8OA_002386"
     CDS             complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="ACG8OA_002386"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFS4977131.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6917..8344)
                     /locus_tag="ACG8OA_002387"
     CDS             complement(6917..8344)
                     /locus_tag="ACG8OA_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4977132.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8375..9589)
                     /locus_tag="ACG8OA_002388"
     CDS             complement(8375..9589)
                     /locus_tag="ACG8OA_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFS4977133.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2917 a         2041 c         1628 g         3244 t
ORIGIN      
        1 atttgttaaa gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac
       61 catctaaagt aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact
      121 gcccttgcag aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg
      181 atggtttata gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg
      241 tgaaaatttc tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt
      301 ttcttggatc gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct
      361 gtacttctag caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt
      421 catataagga tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta
      481 ctgaatcagt taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg
      541 ctgctgtttt atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc
      601 ttgtctcaag aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca
      661 aggataagat gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca
      721 ctatatcttt tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa
      781 tcagcaactt gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt
      841 cgccttcttt ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg
      901 cttttttcat cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag
      961 aatctgtgcc gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt
     1021 agtgatcaat tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc
     1081 atatgctttc tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc
     1141 gacttgttct atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata
     1201 ttctgtatag cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag
     1261 cgcttcctca aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg
     1321 gacaagttca gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat
     1381 gatttctgct tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac
     1441 ttctgaccca tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt
     1501 gatttcattg atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc
     1561 agccaaagcc attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg
     1621 acaagaaacg acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt
     1681 aatgttatat aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa
     1741 taatgagcta tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc
     1801 gtatacgacc agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt
     1861 aaaaaactca gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt
     1921 ccaagcatgc tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat
     1981 gatacaaact ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa
     2041 aggctcattc tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt
     2101 tacaactagt tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc
     2161 taatttttct gattgataac taggatattt ttcttggaac tttttgacat attcttctac
     2221 ttgctgatac ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta
     2281 gtataaacaa ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga
     2341 ctatcttcaa cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga
     2401 acgctatccg tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca
     2461 taataacgac tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg
     2521 actaattcgt gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt
     2581 cctggattga tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt
     2641 cctaattcaa gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt
     2701 tgataggctg gaatactata atcatttgcg atagctgtac catgttctac accatgtgta
     2761 ggaattcgga ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc
     2821 atatcctctt ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga
     2881 atgtcaaatg ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa
     2941 ataatcactg gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc
     3001 aagaaagtac ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg
     3061 aaagtgatag agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct
     3121 tggaatgttt tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct
     3181 gtacttgttt gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca
     3241 gaagttactt gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc
     3301 attttattat cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca
     3361 atcggatgat caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt
     3421 ccattatcca cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta
     3481 ccgttggttt gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata
     3541 gacatctcat tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc
     3601 aatccttcaa aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt
     3661 cctgattctt tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta
     3721 gatgtgtgcc ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc
     3781 acttcacggt aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca
     3841 taagactgat gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga
     3901 aacgcaatcg ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg
     3961 tccttttgca agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat
     4021 aattttttga catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata
     4081 atacattctc tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct
     4141 attctactac ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc
     4201 ttgttatata agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa
     4261 ttaaaaagct ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa
     4321 taggtgaagt tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg
     4381 atagatcttt gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac
     4441 cactcttttt ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat
     4501 aggatttatt tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa
     4561 ttgtttgact gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc
     4621 actgttgatc aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag
     4681 ttggatttga tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag
     4741 ttgttctttt tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca
     4801 caacgtacgg acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat
     4861 ttcagcgata ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa
     4921 atttttataa acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt
     4981 tctactgttg aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc
     5041 tttttgctct tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt
     5101 cttatcttct tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc
     5161 tcgtatgata aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac
     5221 ataatcaatc aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc
     5281 ccaattgaca taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat
     5341 gatttcttta tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat
     5401 tgtatatact ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat
     5461 accattaaaa taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt
     5521 gagctcagaa agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac
     5581 ttgttctttt atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt
     5641 tggtgtagtc acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa
     5701 taattgttca cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat
     5761 tcctgataca cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat
     5821 ttttgtttcc tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga
     5881 caaaagtcaa acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac
     5941 ttttacccca gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac
     6001 tacatctttc aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct
     6061 gaagacacct atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct
     6121 ttttgcaggt acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt
     6181 tgtcataatt gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca
     6241 aggagcaatt cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac
     6301 attgagggag cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat
     6361 ttgattacct ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga
     6421 atgatagatt tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga
     6481 caagattcgc tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat
     6541 cgctcctgca ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat
     6601 gatagaattg aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat
     6661 tccgttgtct ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa
     6721 gcgcacatcg gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat
     6781 acctaattgt ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt
     6841 ttgataatag tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat
     6901 aaccagcttt cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg
     6961 taaatggaat aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat
     7021 gctgtccagc tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag
     7081 tcaaaagacc taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg
     7141 ctctttcaag actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt
     7201 acaacatcag gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc
     7261 ccccgccgct taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca
     7321 tcaaatgagc aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt
     7381 cttctgctgc tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga
     7441 tgacacctaa acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa
     7501 tgtgtcttaa taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt
     7561 gaggtactgt agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa
     7621 ccaatacaaa cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac
     7681 tggcagatgt ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag
     7741 gcaatttcga aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc
     7801 ctgaagcgac ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag
     7861 ctacaaaacc gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta
     7921 aaatagccca gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag
     7981 caccaatacc cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc
     8041 caactgttat ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg
     8101 tgcgactgac ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg
     8161 ctgcgcacaa tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta
     8221 agctgaagcc tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg
     8281 tcatagccaa aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt
     8341 gcatgaatct tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga
     8401 aaaagtccaa tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg
     8461 ccgattttaa aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa
     8521 tcacttgaat ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg
     8581 caaagatccc tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat
     8641 caagtgcatc atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac
     8701 ccgcaatgat cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa
     8761 tggctgccaa tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt
     8821 ttacttttgc aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac
     8881 caaatgcatt gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc
     8941 agccaaacca aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc
     9001 tttctcctaa aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc
     9061 cagctgtcgt gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa
     9121 atccgccacc ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa
     9181 caagtataat atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa
     9241 cggtaatcac gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac
     9301 catgaaacat gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc
     9361 caataacacc gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa
     9421 tcgttgttat cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc
     9481 gtgctaatcc gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac
     9541 ataaaatcat gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct
     9601 cctttctacg attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga
     9661 attaatactt gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt
     9721 tttcgttttt cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt
     9781 ttattttcgt ctttttttat ttttaaagat attttttcat aaggaataaa
//
LOCUS       DAZULO010000098         9673 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000098 DAZULO010000000
VERSION     DAZULO010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.100"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            251..676
                     /locus_tag="ACG8OA_002389"
     CDS             251..676
                     /locus_tag="ACG8OA_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4977134.1"
                     /translation="MHLHRISALAVQIGRYHHSETRAVMEGLEEAIQTTNDCPYRRTF
                     HSDQGWSYQMKAYRNKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCF
                     EELKSAIDSYIYYYNNERIKQKLNWQSPVQFRKTTVTVV"
     gene            complement(971..2104)
                     /locus_tag="ACG8OA_002390"
     CDS             complement(971..2104)
                     /locus_tag="ACG8OA_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4977135.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(2095..2763)
                     /locus_tag="ACG8OA_002391"
     CDS             complement(2095..2763)
                     /locus_tag="ACG8OA_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4977136.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(3219..4205)
                     /gene="lacD"
                     /locus_tag="ACG8OA_002392"
     CDS             complement(3219..4205)
                     /gene="lacD"
                     /locus_tag="ACG8OA_002392"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS4977137.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(4218..5144)
                     /gene="lacC"
                     /locus_tag="ACG8OA_002393"
     CDS             complement(4218..5144)
                     /gene="lacC"
                     /locus_tag="ACG8OA_002393"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HFS4977138.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(5157..5672)
                     /gene="lacB"
                     /locus_tag="ACG8OA_002394"
     CDS             complement(5157..5672)
                     /gene="lacB"
                     /locus_tag="ACG8OA_002394"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HFS4977139.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(5691..6119)
                     /gene="lacA"
                     /locus_tag="ACG8OA_002395"
     CDS             complement(5691..6119)
                     /gene="lacA"
                     /locus_tag="ACG8OA_002395"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HFS4977140.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(6139..6912)
                     /locus_tag="ACG8OA_002396"
     CDS             complement(6139..6912)
                     /locus_tag="ACG8OA_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4977141.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            7077..7556
                     /locus_tag="ACG8OA_002397"
     CDS             7077..7556
                     /locus_tag="ACG8OA_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4977142.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            7613..7915
                     /locus_tag="ACG8OA_002398"
     CDS             7613..7915
                     /locus_tag="ACG8OA_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFS4977143.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            7937..9394
                     /locus_tag="ACG8OA_002399"
     CDS             7937..9394
                     /locus_tag="ACG8OA_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFS4977144.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
BASE COUNT         2795 a         2093 c         1697 g         3088 t
ORIGIN      
        1 ttataaataa ctcataaaaa atattttgac tttcaaaatc acttttgcta ccagaagttg
       61 aggcgaagtg ttttaagcat agttgtaccc tagtttgacc gttaaaaata aataacgatc
      121 gctagagtgc aactatgttt gtttgcccta ccgtcagcgt cggcttggcc tctcacatcc
      181 attaccgtcg ggattatctc cgttacatac ccttacaaaa tacgccctgt aagcattgca
      241 tgaaaccacc gtgcatttgc accgtatttc cgccctagcc gttcaaatcg gtcgctacca
      301 ccattcagaa acacgtgcgg taatggaagg tttggaggaa gctatccaga ccactaacga
      361 ttgtccctat cgtcgtacgt ttcactcaga tcaaggttgg tcgtatcaaa tgaaagctta
      421 tagaaacaaa ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt
      481 agataattca cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa
      541 agtttataac tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa
      601 taatgaacgg ataaaacaga aattaaactg gcaaagtccc gtccaatttc gtaaaacaac
      661 cgttacggtt gtgtaaaaat atcattttag agcatgaaaa aaagagtgga tttctccact
      721 ccaaaaaagt ctaacttttg ggggtcacta catttttgat cctttttatt tgattaatta
      781 tatccagctg gataataatt ttgggggcaa catttctatc ttactattat cttcctaaag
      841 tagtagactt tcttattggg aaaaattaca acaaaaagga gtggcttagt tgacaactcc
      901 tttttgttta caaatcaatt ggatatctat aaattgtatt ttctaacgaa ttttatagac
      961 atctgatttg tcaaattaag ctagacaata tgtctttatc tatgcagctc aaaaatactt
     1021 ccaatggtgt tcgataattt aatgattttc ttggaatatg gtttcttttc gaggcaacgg
     1081 attgaataaa ggtttcatcc gtgtccctaa agtccattgt tttaggtaag ccatcctgct
     1141 tgagcccatg cactcggtac tgatgagaaa aaattgatgc ctgtttctcc tgaggaatgc
     1201 caattccctg atcttttact tcaataatca cgctaccctt ttggtttgaa atggtgacaa
     1261 ttaccagttt tccttcacca tatttgattg cattgtcaac taaattcgaa accgcccgat
     1321 aaatccaacg atctcgtcca tgaatcaggg ttggttgatc ttcagtaaac gcaaacaaaa
     1381 gattcggata attcattttg taggcatcac aaacttccgc acagatcagt gaaacgtcta
     1441 cgacttccgt gctaagatcc tctgagttat tggataatgt caaaatatcg tcaatactgt
     1501 cggtcatccg atctaaaagc attaaatttc cttgatcggc atgttgccgt tctatttcca
     1561 gattcgtacg caaaatagcg atggcatttt tctgctcgtg ggtcagataa ctatttaatc
     1621 gtcgataatc ctccaaatga ccatcgaact tatcctttag cttattaaaa gccgcattta
     1681 atgcctcgtc cttcacagta tttttaggaa cttgatcaat aaacgttaaa tcacgcgtaa
     1741 tcgctaagat ttgattactg taaattttgc gtaaaaccaa ccaaagaata aacgttaata
     1801 acatgacggc agaacaaata accagcacca tcgctggtaa atatttaaat agttcgtcct
     1861 ggtaagcttt atctatcatc gaaatagtca taaattgatt gttggaagat ttcgaagctt
     1921 ccatctgcgt ctctgcttga accgtcttac cctcttccat aaaataaaca tcttcaccta
     1981 aggtagatgc gatactgatt ccctccaaat tatttctcag aaagaagaaa ctcactaaaa
     2041 tcacgcctgt caccaacagt gaaaaagcca gcatcgttaa cgtcatttta aaatttaata
     2101 gcatagctgg tacccctttg ctcgaatggt ttttaagacc tctttttcag ctgcttcagc
     2161 cagtttcttt tttagacgag ccagatgcac tcgcagaacc gacgaaaaag gatcaaaatc
     2221 ttcatcgtag acatgttcag aaatttcctc agacgaaaca accgctggat gacgctgagc
     2281 gatgcacagt aagatatcaa attccttggg tttcaggacc acctctgtct tttgatagct
     2341 gacccttcga cctaatggat caatcttcaa agcaccaatt gaaatctgcg gcagcgcacg
     2401 tccatgataa cgacgaataa ccgcgcgtat tcgtgctttt aattctagta actcaaaggg
     2461 tttgactaaa taatcatctg ctccaaaatc caagcctttt gctcgttctt caatctcatc
     2521 acgtgcagta ataatgataa ctggactttc gacttcttca gcacggagac tttgtaaaac
     2581 agataatcca tcttttttag gcagatttaa atctagtagt atcccatcgt atgaattgat
     2641 gaaggccttc tcttctcctg actcgccatc ataagctaca tctacagaaa tattctcctg
     2701 ctctaggccc ttttttacgg ataaaaccag ctcacgattg tcctcaacca ccagtacacg
     2761 catgtatatt cactcctcat acggatttag taattacact tatgcttttt agcatagtac
     2821 acgctcttct tcaaagtcca cgttcttttt gtgaagattt gaagaaattc atacattctg
     2881 ttaaaaaaca cataattaag aatgatttta ctgtttgaaa atacctagaa tagaaaaaga
     2941 ctggaacctt tcctccagtc tcactacttt ctatttgctt ggttttcttg tatatacttt
     3001 tcaaagttcg ttggtttatg accaataagc ctcgttagaa tactgaaaat tgccatcaag
     3061 tcattaagta ccacatgtaa gaaaatctct ctaaagaaaa accccttcgt tttaactcac
     3121 ttactatttt agaattcgtt cccctaataa tgcagcatga ttttacgctt tatgtataaa
     3181 aaaagactgc aagcaatttt gcttcacagt ctgatttctt atgatagtga gcttgttaac
     3241 acttccaccg gtgtagcagt ttctttcaat acggcattta attcttcaat atttttacgc
     3301 ccttgcgtat tcatccactc catcgctgct tcttttccat caacgatata gctttctaca
     3361 ccatctgccc aagtcgcacg gccgcaaagt acaccattga aggttgagcc ggcatttttc
     3421 gcgaagcgca gtgtttcttg gaacaactct gcttttacac cggcgcttag gaaaataaat
     3481 ggcaggttag tggcttcagc ctgttccttg aagaagttag ccgcttgtgc ctttgtgtac
     3541 acgacttcgt ctgtcccata gccttccaca taattcatat tgacgggtac ttccaccttt
     3601 aacacatcta cgccataacg tggatcagaa aattccttca tcatatcgtt gactttatga
     3661 ggtttcttaa tggcgtattc tttcgaagtc gcatcagaga tgttcgcgtc ataagaaaca
     3721 agctccaaga agaagggtaa gttttcagcc tgacattctg agccgattcg ttcaataaag
     3781 gcatgttttt gattattgat ttcttctgct tcatcgacat catagtaaag taggaactta
     3841 caagcatcgg cgcctgcttc ttttaaacgt ttaactgacc agatcgataa taaatctggt
     3901 aaacggccgg gagttgacgc atcgtaacca gttttttcat aagctaacag caagccggca
     3961 ttttttgctc taacgtcagc tgctggcaag ccatattcgg gatctaataa aatcgatgag
     4021 gcatatttcg ttaattgtga agaaacaatt tctttaaatt caacgatttc tgattcttct
     4081 gcttcgctgc ccttgaattt tgtgatcatc ttcttcaaag ccccgcgttg atcaattgct
     4141 aaggccccaa tcactccatc ttcattgcat agttgcttga tatattgacg tttttcttcg
     4201 ttcatttccg tatcctccta cactttctcc actaagattt gatcatatag tttttgataa
     4261 ttggctaatt ggacatagcc agtcgtcgct tcctgagcat tcagcattcc taaaacatta
     4321 ccctgtttta acacggcttc atccgaaaga ccttgttgaa tcgcagcggt caatccggca
     4381 acggtcgcat caccagagcc aactggattg acgacatcaa tctttggaat tgtcacgcaa
     4441 taaaaagtct cgtgatgttt ggcaaaggct ccgtcgcccc ccattgaaac cactatccat
     4501 tcaatttcat caaacatcga atctgacaag gcggctttta aatctgataa ttctgttgag
     4561 atttctcgtc ctagcagacc agccagctct tcggtattgg gttttattag cttaggcttt
     4621 acactgcctc gcaacacttc ccttaatgct gtacctgaac aatctaaaac gacaggcttt
     4681 ggctgacaaa cttgcagcat ttgtcgataa aaatcagcag gcaatccttc tggcaagctt
     4741 cctgaaaaag aaatgatttc ggctttaaca gctaattttt cgaaatgttg caagaacgcc
     4801 cttgcttctt gttcagtaat aacaggtccg ttttctagga tttccgtctg ttttccgtca
     4861 tgtaaaacag cgatgcaatt acgtgtctct ccgctgatag gtaaaaagtc atgagcaatc
     4921 tcattttgag tcaggtcctt tttaatctct tcacctaaaa aaccgccgat taaaccacta
     4981 gcaatcacct ccgtatctaa ttgcttcaag atacgtgtca catttaggcc cttaccgcca
     5041 gctgttttgc gaacatttct tactcgattg actgtgtcga gcttgaaggt ttctaatgga
     5101 taagaaatat caatcgatgg attcatcgtt attgttaaaa tcatttgtcc acccgcttaa
     5161 tcgtggtatt cgccacgatc ccatttctcg ataaattcat cgaagaaatg cggatcagac
     5221 tgcttgtcac tggctttttc gacgtgatcg attttggcga tcaatttttt gttttcttca
     5281 gtttcttgat aatcggcatt gatgaaggcc tctaatatgt cacagatcaa aaattctccc
     5341 gtaatccgag cgccaaagcc gatcacattg gcatttagtt gttccttcgc atagatagca
     5401 gtcgtcatat cgcgaaccag cgcactgcgg ataccaggta ctttattgac agcgttgtta
     5461 atcccaacac ctgttccaca caaacaaact cctagatctg cttctccgct agtcacggcc
     5521 tctccgactt tttttccgta gatcggataa tgggttctgg taaagtcata ggtccccata
     5581 tccaacactt catgtccttt tgattttaag aaatctgata ctgccatttt tgtatctgtg
     5641 acgatgtggt cacagccaat tgcgattttc atttcattca tccttcctaa ttacgccatt
     5701 ttatttaaca tgtcgacacg tacttgatga cgaccaccat cataatttgc ttgtagaaat
     5761 tccttaacaa tattcaacgc caactcatcg ccaacaattt ttgatccaag tgtgatcatc
     5821 cgagaattat tatgtttacg ggtcatatac gcactgcgct catccgaaac ttctgctgct
     5881 accattcctt taacctttgt tgccgtgata aagctgccag cgccgtagcc atcaaccgcg
     5941 attcctaaac taccttcgtt ttcattcaga tcatttgcga tcgccaaagt tgattccaca
     6001 aaatcttctg aagccgtttc actcttatct actactgtaa taccttcttt gatcaatttt
     6061 ttcttgatta attcctttaa ttccatgccg tcactgtctg aaccaattac tacacgcatt
     6121 tttgtttcct cctatttttt actgaatgat tttagtgaat tgctgataat aactcttcag
     6181 ctcggctgaa agagtagaat ccgtaaataa tttgtcgatc ttgcgcaaat catagaaatt
     6241 ataaaaatcg ctatggtcga atttgtttgc atctgaaacc aaacattttt ctgccgcatt
     6301 atctaaaaca attttttgca aggtaccttc ttcaatactg aaattcgaaa cctccgaacc
     6361 cttcaatcca ttcactccga caaaagcctt cttaataccg attttttgaa cagcttcatt
     6421 ggcaattgaa ccgacaaagg ctccagtctt ttcccgatat agcccaccga tcaaatacag
     6481 ttcaaatctt tttatttcac ttaatagatt gaaaaccggc agactattgg taacgattcg
     6541 gagagatgac ggcttgatgt atcgtgtcat taattcgatc gttgtacccg atccaagaaa
     6601 aatcgtgtcg tcttcttgat tgatatgagc tgccgcattt ttagcgatgt attccttctc
     6661 ttttgtatgc agcttctttt tttcagtatg agaaagctca tttgcttcta tagaagtcga
     6721 catttccaga taatcgattt tttgtgcacc gccatgaacg cgtaaaagct ttttcttctg
     6781 cgcgagttcc tctagatctc ggcggatcgt catatctgaa acattcagta cttcctgaat
     6841 aaatttgact gttatcgtcc catagcgatc acacatcgct aagatcgaac gctgtcgttc
     6901 tgttttcagc atattctgta cctccacgac cttataataa aacatttacg aacatttttc
     6961 aacacatatt gttcgtttat tttcgtttat gcgttgacaa tgattttgta agcggttata
     7021 ctcaacttgt aaacatataa ataaacataa acgaactaat aaaggagctg ttttgtgtga
     7081 atgaattctt tttcaaagac actgtctatg tttctgacaa gcagtcgcag gaagaagtgt
     7141 tcgaggaagt ctatcaagat ctgttaaaga aagaattggt aaccgaagat tttttggaga
     7201 accttttaga acgggagcgt aattatccga cgggattaga tctctcacct gtctctaaaa
     7261 tattgccaga tatcgctatc cctcataccg aaagtgagtt tgtgcgaacc acccgtatca
     7321 ttccaatcaa agtaaaaaat gaaatccctt tctacaacat gatctcgcca agcgatcaga
     7381 tttccgcttc atttcttttc atgatcttga acgaaaatgg tgaagcacag gctggcatgt
     7441 tggctaatat catggatttc attaattctg ttgacgaaga aaaactgttt tcgtttttca
     7501 aattagagga cacagaagcg atttatcatt ttttagaaga acatttttat atatagaagt
     7561 ctctgcctaa ccgcaggata aaaaataaac ttattttaag gggagaatta ttatgatcaa
     7621 aattttagca gcatgtggag caggcgttaa ttcaagccat caaatcaaaa gcgctttgga
     7681 aactgaattg aaaaatcgtg gctatcaagt cacctgtgac gccgttatga tcaaagatat
     7741 caatgaagaa atgctgtctc attatgacat tttcgcacaa attgccaaca ccgacttggg
     7801 ttttgacatt aacatccctg tcgttgatgc aggtgctatt ctttatcgta ttcctgctat
     7861 ggctgagcct gtttaccaaa gtatggaaag cgcaatccaa tcattaaaca aataggacta
     7921 aagaaaggaa aagcatatga atacaattat tgatattgcc aataaagtat ttacccccct
     7981 aattaattta ggtgcggcac cgttaatgac catcgtatta actgttattg ctttattttt
     8041 taaagtaaaa ccgtcacgcg ctcttgaagg cgggctcaaa ttagggatcg ccctgactgg
     8101 gatcggagct attattggaa ttttaactac tgctttttca gacgctatga cagcttttgt
     8161 tgaacgcaca ggtatttcat taaacattac agatgtcggt tgggccccac tagcaaccat
     8221 cacatggggg tcgccataca cgctttattt tttactaatt atggtgatcg tcaatattgt
     8281 catgttagca atgaaaaaaa ccaacacatt agatgtggat attttcgata tttggcattt
     8341 agcaattgtt ggtttgttcg cgatcttctg cggtgctaat ctgtttattg cttctgcatt
     8401 agttattttc attggtgtat taaaaatttg gaactccgac ttaatgaaac caaccttcaa
     8461 tgacttgtta aatgcgccag acagcaaccc gatgaccacg actcatatga actacatgat
     8521 gaatccgatt attatggttt tcgataaaat tttcgacaaa atcttccctt ggttggataa
     8581 atatgatttc gatgctgcca aattgaatag taaaattggc ttctggggat caaaattcgc
     8641 gattggaatt tatttgggga tttttgttgg attactagca ggacaaacac caacgcaaat
     8701 tttctcgcta gcctttaccg ctgccgtctg tttggaattg ttctctttga taggcgcttg
     8761 gttcatcgca gccgtagaac cgttatcaca agggattacc gattttgcca ataaacgact
     8821 aaaaggacgt accatcaaca taggtttaga ctggcctttc atagcaggcc gcgctgaaat
     8881 ctgggctgcc gctaacgtat tagcaccaat catgttgcta gaagccatca tcttaccagg
     8941 aaataaatta ttgccactag gcggaatcat cgcaatgggt gttaccccag cactattggt
     9001 cgtaactcgt ggaaaattaa tccgtatgat cgtaatcggc gcgatcgaat taccattatt
     9061 cttatggtcc ggcaccttga tcgccccgtt cgttactcaa acagctaaag cagtcggagc
     9121 ctttccagcc ggcttgaacg ggtccgccat gatctctcat acaacgatgg aaggcccgat
     9181 tgaaaaattc ctaggttatt tagtcggtaa tgcttctcaa ggacaaatcg aatttgtgat
     9241 atacgctgcc ttagcattag gtgcatacct tttaatcttc atttggtacg ctcgtcaaat
     9301 gaaaaaacgc aacgctgcat acgcagctga aaaagaagag aatgcatctc cggcaacggc
     9361 gcataatatg acttatgcag ccgaagctaa ataagctaaa aacaccccga atgtctttag
     9421 acttcggggt attattttac ctataaaaaa taaatagatg taaaatccat ttcaaaaagc
     9481 atagcccatc tcttgccaca cccaataaag aaattatcaa cgtttatgct ttttttaact
     9541 cctttaagct tgcctttgtt ggtattcctc aacgattctt tttgtgttct tgtttgtttt
     9601 gtgggttctg ttgcaaagtt ttaaatctac tatcaaataa gatagaataa tagaaaaaga
     9661 tagcaggagg aat
//
LOCUS       DAZULO010000099         9604 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000099 DAZULO010000000
VERSION     DAZULO010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.101"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(218..640)
                     /locus_tag="ACG8OA_002400"
     CDS             complement(218..640)
                     /locus_tag="ACG8OA_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS4977145.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            complement(630..2804)
                     /locus_tag="ACG8OA_002401"
     CDS             complement(630..2804)
                     /locus_tag="ACG8OA_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HFS4977146.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2820..5162)
                     /locus_tag="ACG8OA_002402"
     CDS             complement(2820..5162)
                     /locus_tag="ACG8OA_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS4977147.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(5184..7727)
                     /locus_tag="ACG8OA_002403"
     CDS             complement(5184..7727)
                     /locus_tag="ACG8OA_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4977148.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7737..8135)
                     /locus_tag="ACG8OA_002404"
     CDS             complement(7737..8135)
                     /locus_tag="ACG8OA_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFS4977149.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(8216..8500)
                     /locus_tag="ACG8OA_002405"
     CDS             complement(8216..8500)
                     /locus_tag="ACG8OA_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977150.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8524..9522)
                     /locus_tag="ACG8OA_002406"
     CDS             complement(8524..9522)
                     /locus_tag="ACG8OA_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4977151.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2750 a         2034 c         1491 g         3329 t
ORIGIN      
        1 acaaaatacg tttatttttt ctattgattt tagctcaaaa tcgattcaat tcgagtgaaa
       61 tttgcttcaa ttttcaattg gtttttgttc aaattttgaa acatttttgc tcaaaaaatg
      121 agtgaatttg tttcaaaaga aaaacaaata cgatcgaaaa aaatttgaac tcataacaat
      181 tttttgtttt tttcaacgta ttctctttca attttcacta aaaattagga agaaattgat
      241 tcaatgtctt ttcaaaaaag aatgtttttc tctcaatttt tttgacgttt ctatcatgaa
      301 tcacgagaaa acttgggaca cttttaacat ggtaacgttg taaaaaagtt tgaagttctt
      361 cttgtcttgg ttcttttctt gtttcaattc gatatacatt cctagagaat tttttgtcgt
      421 atctttccaa gaaagattca acttcttggc attttttaca atcatttctt gctagataca
      481 caaaaaatga ttgattcgtt ttttgaagtt ctagtacttc ttctaaatta ctattgatta
      541 gttttccatt tgattcttcg ttagttttta tcagtttaaa acaaccaaat aagaggaaac
      601 taacaataag tatcctaaca ataactttct tatttcgcat attgtttctc caaatttggt
      661 ttccgaatat agacgactcg cggtggttcc tggacgtctt cagctgttcg atacaccaca
      721 aattcgcctg ttctcaatgc tcgaatgcga ttcggattga atttaaagcg gtctacgtct
      781 cgtttcgtgc ctctttctcc tgtccaatca acctttccca tatcagaata acctgcttct
      841 tgtgtcgtca tttccgttaa ttcaatgtct tcttctgtcc ccattaaatc tgcccaataa
      901 tcaatttctg tcgggtcatt tgtcttccca ataaagaacg tattcacatt cccaatcaac
      961 tgttttcgaa ggacaggatc aattctatca atgtccgcaa gcgtttgagg ggcatacgcc
     1021 gcatgcaccc cagcgcctcg tcccttattg actaagtcta taaggttatc atttagataa
     1081 gaagctggtt cgtcaaatat tactaggact ttcgagcgat tggctgtttc tcctgcttgt
     1141 ttatacactt ctgacgcaaa gaaattaata tctcccacaa gcatttgagc caatgtccga
     1201 ataaactcac tataaatcaa tccgtttaac gagatataaa caatctttcc ttctttaaca
     1261 acttcttgaa tatctaattc tctttttcct ccagaagtat caaagagttc accaagctca
     1321 ctatccaata acatattgat attggtctgt aaattctcag aagtcgcata caaataaaac
     1381 gggttcttct tatgttcgta acgaacaaac aaccgtttaa acaacgtttg ctcgatttct
     1441 gtcatgagag aaatgttttc ttttaataat agataatacg tttctaagtc aacggtttta
     1501 cgatcatgtt caacgattcg ctcttcactt aactgttcca catcggcaaa atcacgttct
     1561 gttttgtcca tcgcttcatt ctttttcgtt tttttcttcg tggttttctt cttttctacg
     1621 accacttttt ctttaaataa atctggattt ttcaactgaa tacgatccgc aaacaaatgt
     1681 aagacgtttc gtggcaataa ataatgttgt aaccaaacta gatttctttc aattttatgg
     1741 ctcttttgga actcgtctaa aagctgaatc gttacctgta ataacgcttt ggctgctgtc
     1801 gaataataca cgctttctgt ttctgctaga ttggttaatt tatctcgaat ggcaatatca
     1861 ttgccatttt ttacaggatt ataacgcaaa tcgccaccgt ccacaaagac ttcaatcggg
     1921 cgattgtatg cctctgccac ttcttttaac gcttgtaaag tctctcctgc tcctttccca
     1981 tcgactaaga taacggggat acgaaactta cacgcatgtt ctgctagtaa ttggatcacc
     2041 gtcgtttttc ctgaacctgt agtcgctgga ataatcacat gttggttcaa ttcactaaag
     2101 ttcatcacat aagggagccc atactcatct gttcctaata gaatcacttt ggctaatgaa
     2161 gccagttgtt tattccgcaa agcaacgtct tttatgacaa ttgctacctg gtacgcttgc
     2221 tgatactctg ctgtatattt ttgggactcc tgaaatttat tcttccgacg ttccttgtat
     2281 tctttactat ttagcaaagc tgtttgccgt tctttctttg aaacaacttt tcttttttca
     2341 ataaaagacg catacgcaat caaacaactt cctgcacctg tcccacctaa aaggagggca
     2401 aaaatcgttt gtccattaaa ctgcaacgca tgagaaaaca agtttaatgg aaataataag
     2461 taacacgaca aataccctct aggaagaaca aaaataaaac aaaatagatt taatagcgta
     2521 aaacctaacg caaaaataaa aaaaagagag cttcttcgtt ttaagaagct ctcatactca
     2581 aagtcataaa tatgtgcacg tgtctctttt ttatctatca taaagacaat cgctaaagga
     2641 atactgccta caataaaaaa tggatataga caaacaccta ataaagtgac gataaaataa
     2701 agttgttgaa accgactttt tggcgtttct ttttcttgtt ttcttttgcg atcgacagaa
     2761 aacatttgct caaaatgtgt cggtcgtttt ggtgcattca tcatactatc gatttcctcc
     2821 tatcgttttc gttgtttcat ttctcttcga taaatcggtt gtttttctcg ttttcgtttc
     2881 atttttgctt gagttgctcg aaactcactc cgtgtcttcg gtgcttgttt tggtgatttt
     2941 gtttgtttca tggttgagta tgctttagat acggttgaag gcgctttggt ttgtgctgtt
     3001 actcgattga ctgctgacat atgttcccga accccctctg gacgtcgaac agatttcggt
     3061 gtggtagaac ctgttgctcg tggtacattc ccttttctta gtggtttttg taccgcatgt
     3121 ttttgtcctg caactgtccg ggttttcatc gaacttgttt ttgttacggg agtaagtgcc
     3181 tccttccgac gacgacttgt ttgtccctga cgacgttttc gatcgttcaa ttctgttcta
     3241 ggcgtatgct tctggccata agacttgtgt ttttgctttg gattcgctaa tgacaccacc
     3301 attgcagaac cctgattgcg atacggacta cgagacgcac cttttctact tctttctctt
     3361 agactaggat tgccttgcaa ttctcgttgt aataaatcgg ctgtttttct tgcctgtttc
     3421 tccattgctg ttcgttgcat acgttcttga ctttgctctt ctgcttgttt cgcttgatca
     3481 tgcaagtctc ttgctttttg tgccatacgt tccatacgat ttggtgcttt atgacgaggt
     3541 tgttgtgcgg attcacgccg aaaagcactt cgtgtggctt gtttcgccgt ttgtggcata
     3601 cgaacgtctg agccaccccc acgttttcgt tgacgttctt gtctcttccg tgcgttttct
     3661 ttttcttttt gtttctgtgc ttttctcgct tctttgcgtt ctttcaaccg ttgtttggct
     3721 tcccttctca attttgcttc tgccccaaat ggatttcccg caaaagctaa cgctcctcga
     3781 acacccattc ttccagtaaa tagactgcct aaaaagcctc ggaacaaata aattcccaac
     3841 ggcgcaaaca aataaacaat gatcaagaga attttttccc atggatcgtc ttgtgtacta
     3901 cttaattgga acccgtaact aagaaaagaa gaaaaaagga tacatgcaaa acctgtcatt
     3961 ccttttaaaa acgtcaaaat accaaaaact ttggcatagt taccaaacac attcattgtg
     4021 acaagaaaca atcctaaaaa aagtaagaac ggcaacatcg gtactaacaa tagttgcata
     4081 gttccaataa tcaatcgaaa catacttaag acaaaataaa caacggtttg aatcagattc
     4141 acgacaaaat aaaacaaggc aaatgctgta tttttccaac tattgtaata ttgtatgttc
     4201 ttgtttttca gtgtatctgc ttcatactct gtgactttgt catgaatttt ctcccctatt
     4261 tttgtaccta aatcattgtc caataaaaga ttgattcgat catattcttt tccagcatat
     4321 ttgactttct tctcacgaat tgtgtctaca ttggttgtgc cataatttaa caacagatat
     4381 ggttcataaa cattactata aaagacccga gccgccatca atttgcctgc cgtttccact
     4441 tttttatccg ctgttacctc ttctgtttct tttgtttcat aatttgtttc ttcttcaggt
     4501 gttaacactg gattgacctt cacgaacgcc gtttccactg tagaatctaa agaaaaaaga
     4561 acatcaaaaa gattcttatt tgtcccagaa tcttttaata cgcttaaccc agtaaaaata
     4621 aggatcgtca taagaaagac acggagtgct tgtttcattc gagcctctcc agcatatttg
     4681 attgccatca taattgccga aaaactgatt ccaatagttc ctgcaatact taacatattg
     4741 tccgcaattt tttgtgttaa gttttgaatg ggttctcgaa tggcggaaat aatatctaat
     4801 tcaaagagaa attcaaccat tgaagcattg aattctccta acatttttac gccactccaa
     4861 acaaaatttt tcatcactcc tgcaacattg tacgatagct tttttttaag gttcattgac
     4921 tcatagtcat tggtcatcaa ttcaaacgaa ttctttttgt aatttttata caattgatcc
     4981 acatcagtcg ttacttcttt tacttctact ttatcgttgg cattctctcc ttcagaacga
     5041 tcggtttcag tgacacccgc accatcattc atttgatcgg cagaaacagt agccccttgt
     5101 agacagaaaa aaagaccccc taataaaagg agcgtccacc atttcagaga tttttttcgc
     5161 aaaaaaacca cctctttttt gttttatcgt tgtttttcat tttctaacat tgcttgaatt
     5221 ttttcatctt gattttcagt agaagaaaca gccaataacc aactatcaaa caacacatca
     5281 atggcaatcg gttgatttct tcccaaatga tcctggaata aacaggttcc tgtttgtaat
     5341 tgttccatca tttctaaatt ggctgaattc acttccatat caaagaagga caatagtttc
     5401 tctcgtgctt ctttttgttt cggacgaaac gcaaatttgt aagaaattaa ctctttttta
     5461 tcttcttgat cgtaatcttt aaacgcttgc acaacaaggt aaatatccgt attattcgca
     5521 cgtccttttc gcaagctatc atcaatcaaa tagcttcctt gtgcggtatc ttcataccct
     5581 tttgcctcgt cataaataat cgcagcgtct tcttctttgt ttgtagaaaa gatatacgta
     5641 aacttcataa tgacttccat aattgccatt cctgcaagtt gttcgctatt taatcgaccc
     5701 gaatcaatct ctttttgtgt cggaatgcgt attccttgtg tccctaacac gttgatttgt
     5761 gaatcaaaac gtatcggcgt actgtgctca ttcccaatga gaatctttcc taaaccaacg
     5821 ctatgcccta aaatcaattt agctagttct ttgtcacttt ttcgaatttc ttcgataact
     5881 tgacttaacg aatgcttacc tggcatggtg taaactacat cgatggcgtc ttggatcacc
     5941 gttttcttgt ggctcgctgt ttctcgattg gtatctactt ctcctaacat tcctaacaca
     6001 tttttagcgg tttgaattgc ctcttctttt ggtaaaaaca atagaggatc aagcatccct
     6061 cgacagcttt catctgaaga taacgaaata aaattaattc gttcatacat agccacaaac
     6121 tctggtatgt gtccgaattt ttcaagtgcc tttaaaaaga acttttgggt ttcatttttc
     6181 ggatcaatat aaagaatttt ttgacctaaa aacatcagcc ataaaaaaat atattttaca
     6241 aggaccgatt ttcctttacc tggtggacca ataatcaacg tatttccatt ggtatgttcc
     6301 gcgccttcaa tacttttctt tgttaaattg ggataatacc atactagttt actagatagc
     6361 gctaacgctt ctttcgcaga tttaaacttt tttgcaataa caatccgtcc taaaggaaat
     6421 cctttcttat ttcctacttc tttctttaag tccaagccat aatctgccac aaaacctgtc
     6481 gtagcgacct gctcgtaact acgaaaacgt tgcgtacttc caattaagca ttgattaaac
     6541 aacgttagct gatccgctac cgatcgataa aatttgaaat ctgtagactc aatcgcaaat
     6601 tcaatatctt tgactcgttc ttccaattct tcttttgaag aagcagatac aaccagcgtg
     6661 acggtagaat aacacaatcg tttttctttg cgcttcagct tatcttttac atccattaac
     6721 cgttctgtac caaaatttaa tacatcgtct gaatctaaaa tgccgtcaac ggtctcatat
     6781 tctttttctt gttcataaat ccgtccagcc attttattaa tttttcgttc atcagatttt
     6841 gaatgctcaa atcgaagtcg aatatgcgta tcaattggaa aagataagct atcctgaaga
     6901 ttttgaataa agctactccc atacatggct aaaggagcgt ccgtttgact taaaaacgtc
     6961 gaataatgcg tttgattcaa ctgttcaatc gcaatgtatc ctgcttcatt cgaaatgacg
     7021 ccctcgcaaa tttccattgg cgtaagctgt ctttcaggca atttttcttc tgatcgatgg
     7081 aaaaaataat aataaatttg tccaatctct tgggcagata agcgctctat cggcttataa
     7141 ttggacaatt ccgtatagag ctccttttct aatgtttcat acgttggtaa cacactactt
     7201 ataggaactc gttgtcccgt aatacgatag ataaactttt taaaaacatg cttaagcaat
     7261 tgaaaatatt cgatcggatc agacactttc atttctgtag tgaaacgcac aaacagatac
     7321 gttcgatact cgtttaattg cacttcgtct tttaaaatct ttcctgcctg ttcaaagtac
     7381 actttcccaa gatctgcgca ttctcccttt accaaatctc gaatggtttc ttcaatgtgc
     7441 gtatctaggt taaattgacg tggaatattg ataaaatgat agtcatattc atcatgtcca
     7501 agtatgcctt cgccatcttg aagatagttc ttgaaaaaat caatgtcatt taaaggaaat
     7561 acttgggaag aaagttgata gcctacccac acatcatcat ttttggtcaa aacaaaattt
     7621 cggataatgg cgtctatcgg aaattctaat cgaggaagct tcgttttttt ctttttgaac
     7681 tgccaaatcc cgataataaa gacgaccaaa agaaacaaaa acgccattta ctcgactcac
     7741 tccttccatt tgtgtaggga atacgtcact ttttcatgga aaaacggcac atacatttca
     7801 tgttcaaagc gttgtttccg tttctgataa taggacgttc gcccaattaa atactcgaaa
     7861 aagtgtttat tgtcccattt cattgaaaac aatgtccaaa cgagaacacc aatcccaaaa
     7921 atcataatca accaggactt tcgaaacagt gtctgcaaga aactaatttg attcacatac
     7981 gcaatcaaaa ataggatcaa aagaatgaag agaaataccc cacctatgac taattgttcc
     8041 atgcgaatgc cattagaaat ccagggaatt ttttgtcctt taatcccgta aattttgtga
     8101 gggtgcttaa actcctttgt atagttgtat tccatgttca ctctacctcc ttttacaaaa
     8161 aaagaagcga ccagaagttc taatcttctg atcgcttttg cagtttgtct ttttcttatg
     8221 ataattgtat ccagggcaca atcatttgcg ttacccccac gttctcttca ctatccttaa
     8281 taaagataat gttctttagc cattcaccaa tatcaacggc attcgataac aataagacga
     8341 gcattatcaa aataaagacg ccaataatgg caaacgctgc ccgcgcaaag ccttgggtcg
     8401 cataagcacc aaccattaga acaatcgcaa cgattgccaa tagccaaatg acttgtccag
     8461 atacatcatc aaataacgat ttaagatcaa aaagtttcat ttccattctc ctctaagttc
     8521 attttattca tttaacgtag taacaaaata actatcgcca cttgttttta cttccattga
     8581 aaagtttgat tggaactcgt ttcctttctc aaaatagaaa cgatacttcc cttgcaaaaa
     8641 gtagtttcct tctttatttt ttactgcatg atccagttgt acattgatca attcgccgcc
     8701 ttctaaccct ttttcgttag aaatcaatgc caatttttca tcatttttga catataactc
     8761 aaaaaaccgt ttcacaaatg cttccagctt gatttcttcg ctttcagtga ccgcttgccc
     8821 agaagaataa aatcgatttt cctgatagtt cacagaattg gtattcttac tctcagaaag
     8881 attcaaaaat tgaacgccat caagtacttt taaaacattg ttttgataac tgactggaaa
     8941 agaaagttga ctgcttttcc aatgttctcc ttctgtaaaa gtaacctcat aaaataggcg
     9001 atacattgct tgtgtcaaat cgatttcttc tagtttaata aagcgaacat ctttcgctac
     9061 tcgattggta gccgtcgtta caaagcctag atcagacgct tccaatcctt gtgctaaata
     9121 atgattgacg gaagcctccc attcgtttcg tttttctggc gaataagtaa ataacctttc
     9181 aataaaatgt tgtccctcgt acttcaattg ttccgtagaa gacaaggttt ggttgacttc
     9241 agttaacaat gtttctggat tgacttgttt tttcactgct aaacgcgtta atgtatccac
     9301 tcgtccaaag tttaggagga gcacgacacc cattaacaga aacaaacaaa aaaaagcgcc
     9361 tgaaaataca ctcatggcac ttttttgcga aaattggatc attcgatctg acggttctgt
     9421 tttctttttt tggaacaaaa aacgtttctt ttgtttcttt tccttctgtt gtttcggtgg
     9481 cttagtttgg aacgcttgtg gattaaattt tgcattactc atgagaaatg ccataccgtt
     9541 ctaacaataa tttcggtatt ttgtccacaa tcactacttc gtggacaacc acacttgccc
     9601 cctc
//
LOCUS       DAZULO010000100         9360 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000100 DAZULO010000000
VERSION     DAZULO010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.102"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(91..759)
                     /locus_tag="ACG8OA_002407"
     CDS             complement(91..759)
                     /locus_tag="ACG8OA_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4977152.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            893..1180
                     /locus_tag="ACG8OA_002408"
     CDS             893..1180
                     /locus_tag="ACG8OA_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977153.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1596..3134
                     /locus_tag="ACG8OA_002409"
     CDS             1596..3134
                     /locus_tag="ACG8OA_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFS4977154.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3131..4096
                     /locus_tag="ACG8OA_002410"
     CDS             3131..4096
                     /locus_tag="ACG8OA_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4977155.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4083..5405
                     /locus_tag="ACG8OA_002411"
     CDS             4083..5405
                     /locus_tag="ACG8OA_002411"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4977156.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5468..5572
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5646..6389
                     /locus_tag="ACG8OA_002412"
     CDS             5646..6389
                     /locus_tag="ACG8OA_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4977157.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6404..7396
                     /locus_tag="ACG8OA_002413"
     CDS             6404..7396
                     /locus_tag="ACG8OA_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4977158.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7408..8145
                     /locus_tag="ACG8OA_002414"
     CDS             7408..8145
                     /locus_tag="ACG8OA_002414"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4977159.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8135..8857
                     /locus_tag="ACG8OA_002415"
     CDS             8135..8857
                     /locus_tag="ACG8OA_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS4977160.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8941..>9360)
                     /locus_tag="ACG8OA_002416"
     CDS             complement(8941..>9360)
                     /locus_tag="ACG8OA_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFS4977161.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3068 a         1607 c         1972 g         2713 t
ORIGIN      
        1 ttttttataa agaagatttt actgaataat atcattttag tttacataat gatatttctg
       61 taaattaaat aaagtagtga aaaataagaa tcagtgctct aactgttcaa gaggatcaag
      121 tggttcaaat gtttctgtag tctgttttgc ttccaattgg ggcaaaacag atccaatttc
      181 cagatacttc actttattca atgtataaga tagaatagaa cagctaagta atgtatacag
      241 aaaatgtgtg agatccaaat aaagcaagga gacaactaaa ataccaaaag taatcaagaa
      301 aagacttttg ccaatcttaa tatgaaaata tttttcaatg gcttgataaa tgatatctgt
      361 tccgcctgtg cttccgccga atcgaaagac gagaccgcaa ccaatgccag aaagaatacc
      421 tgtcatcaaa ctattgatca gtagactatc tacagcaaaa tgtatatgaa ggctctccca
      481 taaatgtaaa aagaaaacta gcgcaaatgt tccgacgatc gtccggagaa aagcgtgttt
      541 tccaaaaaga aacaatgaga taagcaataa aggtaaatta aaaagcagtg aagtgacaga
      601 aggagcgaat ccaaaaagat tatgcaaaag caaagcaata ccagtaaccc caccatctgc
      661 cagcttagat ggaatattga ttcctgtcac agctaatccg tagattcctg cgcctaaaat
      721 gattccgcac aattctgtaa ataaatttaa ttttttcatt tctagcctct tttcttttga
      781 aaattttcag caacttcttt attatactga cttatactct tacttcaaga ggaaaatgaa
      841 aaaaaattat ttttggctgt ttctttttat ttatcaaata aggagtggaa aaatgcgagc
      901 agcagactta ttgacaattc ttacaaaatc acccaaaaaa acagagtatc aaggagtttt
      961 tttggctcaa gaacaaaaac tggtgcctgt gacaatggtc cagataaata aagcaaatca
     1021 atttatcttt ttatttgaac acggcaaagc accacttgct atgaaagaca tctttctttt
     1081 tttgatgaag aaccgtgaga aagctgttta ttatcagtca aaagaagaac tcattcctct
     1141 ttatggagtg aaagagatag aaaataaagt ggtgatataa gtaaaaataa gaaaacaaga
     1201 cgtttatgat gtcaaaacaa ccatttttta catttttggc aaaaaagtgt catgatacag
     1261 tataatattc gttgaacagg ccaataaagg tgttctcccc cacgagattt ctactttatt
     1321 ggctgtttct ttttattaaa ataaaagttg tgtgtgtaaa aaagatatag ctgattccaa
     1381 gtgacaattt cgtatagatt tttatggtat tcgtagtgaa aaaacgagaa ccaaaaagtt
     1441 gatccgactt tgttgcttgg attctttttt tgtcagcata tttagaggta tttttttctt
     1501 ttttttgttc gtgctgtaag gttaaggcaa gacaagttag ttgggaaaaa ggaaagaaat
     1561 gtttatactt aaagaataac agaaaggaag gtcgcatgaa taattttata caaacattaa
     1621 gtgaaagaaa aggcgagttg ctggaagctg catttcaaca tctgtccatt tctctcattt
     1681 ctttgctgat tgcagcactg attgctattc ctttagcgat ttgggcagcc aatcataaaa
     1741 aaatggcaga agtcttattg cagatcacgg gtgtgctcca aacgattcct tcacttgcct
     1801 tattaggttt acttatccca tttgtaggga tcggaactgt tccagcattg attgcacttg
     1861 ttatttacgc tttgcttccg attttccaaa acacgtatat tggtctagct gaaattgacc
     1921 cgtctattga agaagcagcg gtagcatttg gtatgtccag aatgagacgc ttgctgaaag
     1981 tagagcttcc catcgcatta cctgtcatta tctcaggtat ccgaactgcc ttagttttaa
     2041 tcatcggtac ggctactttg gcagcattga tcggagcagg tggattaggg acatttattt
     2101 tgttaggtat tgaccgaaat acacctgttt tgacattgat tggtgcgatc agttctgcct
     2161 tgcttgcaat tatattcagc ggactgattc gctggttgca gcataagaaa cctcgatatt
     2221 ccatcattac tttagcagca gtggtgtgtt tatttggcgg cgtttcttta tatcagtctg
     2281 ccttgtttaa aaatgaaacc atcacgattg ctggaaaatt aggagcggaa cctgaaatcc
     2341 tgatccaaat gtataaagaa ttgattgaag acaacacaaa aacagaagtt gaattaaaac
     2401 cgaattttgg caagacgagt tttttgttca atgcactgga tcaaggtcaa atcgatatct
     2461 atcctgagtt cacaggtact gtcctagaaa gcttggtgaa ggtacctgaa gcaatgaaaa
     2521 gtgctgattt gacaaaagaa aaaacatatg acgaagcaga agaattattg aataagcaat
     2581 tcgatatgac attattaaaa ccaatggctt accaaaatac gtatgccttg gcagtcaaga
     2641 gatcttttgc gaacgaacat caacttaaaa agatttccga tttggcagct atctctgatc
     2701 agatcaaagc gggattcaca ctggaattta ttgatcgttc agatggatat aaaggaatcc
     2761 aagaagcata tggcttgaat ttttcaagtg tacaaagcat ggagccaagt ttgcgttacc
     2821 aagcaatcaa taatggagat gtcaatattg tggatgccta ttccacagat agccagctca
     2881 aggagtacga tctagtgact ctagaagatg atcgagggtt attcccagct tatcaaggcg
     2941 caccgctgat gaaaaaagat tttgctaaag aacatcctga aatcgttaaa gcactgaata
     3001 aattaagcgg aaaaataacg gaggatcaaa tgattgatat gaattaccaa gtcaatgtcg
     3061 aaaagaaaaa gccgacagaa gtagcacgaa tcttcctgaa aaatgaagga ttagtaaagg
     3121 aggaagatca gtgactaaat taatcgagtt caaacatgtg caaaaaaagt atgatggaaa
     3181 gtatgtcatc gatgacctaa atctggcaat caaaaaagga gaaatctttg ttttggttgg
     3241 tccttccggt agtggaaaaa caactacttt gaaaatgatc aatggattat ctaaaccatc
     3301 agcaggagac atctacttca aaggaaaatc gttaaatgaa tataatctgc agaaaatgcg
     3361 gtggaatatg ggatatgtgc tgcagcaaat cgctttgttc ccgaccatga cagtcaagca
     3421 aaatattgaa gtcatccctg aaatgttagg ttgggaaaag cagaaaagag cagaccgtgt
     3481 agatgaactt cttcaaaaag ttggcttatc tcctgatatt tatcgagatc gtatgcctag
     3541 agagttgtct ggaggagaac agcaacggat cgggatcatc cgagctattg ctgcaagccc
     3601 agatgtgatt ttgatggacg agccatttag cgcattagat ccgatttcaa gaaactcatt
     3661 acaagaattg gttttgtcct tacacgaaga attgggcacg accatcgttt tcgttacgca
     3721 caatatggaa gaagcaatca agctaggtga tcgtattgca tttatgaagg atggagaaat
     3781 tatccaatgt gacacaccag agcagttatt gatgaatcct aaaaacgact acgttcggca
     3841 tttctttgat gaaccgaaac agacgaaaga gtggcgagta gaagatttag tcgtcaatgg
     3901 atatttctta aatgaaatac cagaaaatgc tcgtcaacaa gtttgctttg atacgcctat
     3961 gaaagaagta tattcgcttc tttcggtgta tccaagcatt gtgatagtag aaaagcaaag
     4021 aagtattggg tcgctgacaa gtaaagaaat ctttgctttt ttaagtcgag aggaggaagg
     4081 aaatgaaaac atatgatgca atcatcatcg gttcaggtgt gagcggctta agtgctgctt
     4141 atggattgaa agaagcaggt aaaacagtac tagtggtcga agaagatcta tggggcggga
     4201 cttgtcctaa ccgtggatgt gatcctaaaa aagtcttgtt aagtgcagta gaggcacgta
     4261 atcgggtaaa acaattgagt ggaaaaggtt ttaacgagat cccaactgcc aactgggaag
     4321 aacttcaaaa attcaaacga acatttacgg atccagtccc agaaagccga aaaaaacaat
     4381 tagcagaagc tgaaatcgat catctttcag gaaccgctcg ttttttggat gattcatcta
     4441 ttgaagtcaa cgaagaagtc ttccatgcgg attacttagt tttggcaacc gggcaaagac
     4501 ctacgatctt accagtggag ggaaaggaat acttgaaaac tagtgcagac tttttatcat
     4561 tgcctgtact gcctaaagaa atcattttta tcggtggtgg ttatattgct tttgagctag
     4621 ctacgattgc caatgctgca gggagcaaag taaccattgt ccatcataat caacggccat
     4681 taaaagaatt tgaggcgtct ttggtagagg aagccgtcca tcaaatggaa gcatctggca
     4741 ttcagtttgc ttttggtgtt gaaacccaaa agatcatttc agaaggtact cgctatcgtt
     4801 tagtcggaaa agagacggaa ttagtagcag atatgatttt ttgtgcgact gggagacagc
     4861 cgaatacaga atctttagca cttgagcaag cgaatatcgt ctttgataaa cacggaattg
     4921 cagtcaatga ttatctgcaa acgagcaatc caaaaatttt tgcgtgtgga gatattgttt
     4981 caaggaaaac cccaaaattg acaccagtcg ccacttttga aggaaattat gtagctaagc
     5041 ggataacgga tgcaacaagc gaacctatca aatatccgat tatacctacg atcgtctacg
     5101 ccagtcctaa acttgctgaa gtaggagtta caaaaagtca cgcctcttcg tcagatcaag
     5161 tggtagagat ggatcttacc agttggttca cctatcatcg tgtcaatgaa ccagttgcta
     5221 aggctgaatt gacctttgac cagcaaaatt atttgattgg tgctgcagtg attagcgagc
     5281 aagcagacga attgatcgat gatttgacac tcgtcatcaa tcaaaaattg actaaaaaag
     5341 agttggatag ctatatcatg gggtatccga cattggcaag tgatctctcc tatttattga
     5401 aataatcagc atgataacta aagcgcttgt tgctttttcc ttcttgtgct acactttaaa
     5461 aaatgaatac ctgtttctgg ggatgtcgta gacgacatga gagtaagttg tgaacttaga
     5521 cccattgaac ctgtcagtta atactggtga agggagaaac atagcagacg cttgaagcgg
     5581 gttactatgt cgtgcccatt tcaagcgttt tttgttattc atattagaag aaaggaggta
     5641 acaaaatgaa gaaaaatctt tatccagcag tcagtatggc attcatttta attatttggc
     5701 agattgttgt catagtttgg aaaatacctg cctttatttt acccggacct gctgctgtat
     5761 tttctgcatt agcggcagat gcacaagtat tactttccca ttctgtcacg acattaaatg
     5821 aagcagtgct tggtttggta atagctagcg ttctttcttt tattactgcc ttggcaatgg
     5881 attattggaa gtttttagaa atgtcttttt atccgttact agtgatttca caaatgttgc
     5941 caatcatggt gttagggcca ttacttaccc tatggtttgg tttcggtatg cttccaaaag
     6001 tgatccttgt tgtcttgata agctattttc cgatcgtagt ttctttttcg gatacattga
     6061 ggaaagtatc aaaagagcaa atcatctttt tgaaaacgat gggggcagat acagggaaga
     6121 tttatcgaat atataaaata ccagcgggtt tatccggctt cttttccggt ttaaaagtgg
     6181 ctgctactta ttgtgtcagc ggagcagtcg ttggagaatg gctaagcgct caagcaggct
     6241 tagggtatta catgatacgc gtaaaaaaca gctaccagat cgataaagtt tttgctgcga
     6301 tcatttgtgt cattctattg agtttattgc tgaatggcgc ttgttcatta ttgaaaaaag
     6361 ggtattatcg tatgctttat attttttaag agaaggggat caaatgaaaa aattatggct
     6421 tttattaccg ctactgttgc ttttatccgc ttgtggcact gcaaaagaaa caagtagcaa
     6481 acaagaaata aaggatttga aaaaagtgac acttgttttg gattatgtac cgaatactaa
     6541 tcatactggc atctacctag caaaagaaaa aggctattat aaagaggctg gtttaaatgt
     6601 ccaaatcatc gaaccaggag ataattcgac aagtattggt ctagtaggtg cggacaaagc
     6661 acaatttggt gtaagttatc aagaagatgt cacttatgct cacgcagatg gacagaatat
     6721 accagtcaaa gcaattgcta cagtgatcaa acataatact tctggattcg ccaccctatc
     6781 agacagtaac atccattcac caaaagattt tgaaggaaaa acatatgcag gttggcaaag
     6841 tccaagtgaa gaagctgtct taaaagctgt gatggaaaaa gacggcgggg acttttcaaa
     6901 actgacaatg gtaggaagca atggagaagg gccagaaagc ttaggaaaat cctcagatat
     6961 ccaatggtac tttgaaggtt gggatatgat caaagcaaaa gaagcgggta ttgaactaaa
     7021 ctatatccca ttgaaagaat tagatgaacg gttagattac tatactccgg taattattac
     7081 gaatgatcaa ctgatcaaaa gtgatccaga attggtacag tcttttatgg atgccacgaa
     7141 aaaaggctat caggaagcta ttaaggatcc aaatgacagt gctaaactgt tgcaaaaata
     7201 tgcaaaagaa aatgatcgga cgtttttaga agaatcacaa gcattcttgt caaaaaatta
     7261 tacggatgat cctgagaatt ggggattgat ggaagaaaaa gtttggcgca attatacagc
     7321 ttttatgcag gaaaacggat tgatcaagca agatgtacct tcgcaagaac tattcactaa
     7381 tcagtttata aaataggagt gatagatatg tcaatccaaa tcagccactt gaatgtagtc
     7441 attgaaaacc tgaaaatatt agaagacatc catttcacta tccctaaaga tacctttaca
     7501 tcgattgtcg cgccaagcgg agccggtaag tcaacattgc tgaaaacttt aacaggagtc
     7561 cagcctatga cttctggaac gatcaaagta gacgatcaaa aagtaactgg tctccataca
     7621 gcatttagtt atatgccgca agaagatatg ttacttcctt ggctgagcgt ctatcaaaac
     7681 gttacgttat atcaaaaaat caatcatcta acaatcaatg aagaacaagt aagggattat
     7741 ctggatatct ttgggttaaa cggctatgaa cattttttgc cggaacaatt atctggcggg
     7801 atgaagcagc gaacggcctt gttacgaaca atcatgaatc cttcttctta cttgcttctt
     7861 gatgaaccat ttggagcgtt agatgctatg accagagggc agatgcaaga ctggatgttg
     7921 aagctgccta aaaaagcaaa gcggactact ttacttgtga cgcatgatat cgaagaagca
     7981 atttatttat cagacaggat tcttgtgctt tccgcccgtc cggcacatgt gattgctgag
     8041 attcaagtgc ctgaaaaacg acggtcacgt gaatggctac tgcagcaatc agagttgaag
     8101 caaacgattt atcagttatt ggcaggtgaa ttagatgtta agtgatgctc attgtcattt
     8161 agatgggagt cagagtttag cactccttca gcaggaacac acgattttga cgatcatcaa
     8221 ttgtgattcg ccagaagaat ggaaggaaaa cagacagctt gcagcaagta agactcaagc
     8281 cttaagttac ggtatccatc cttggaaagc agatagctat acgtttgaac aagttgagcc
     8341 ttttttgaaa aaagcaagga tcatcggtga aatagggtta gataatattt ggacgaatgt
     8401 accgatgact acacaaaaaa aagtatttga gcgacagctt gcttttgcag ccataaatga
     8461 gaaacctgtt gtactgcaca caaaaggatg cgaaaaagag atcttaaatt atatccaagc
     8521 ttaccctaat cactatttga tccattggta ttcttctttg gactatcaac aagactatat
     8581 tgatttaggc tgttatttca gcattggtct tgatttgaaa aagaatccag cagtctggca
     8641 actagctaaa gcagtcccaa tcgaccactt acttattgaa acggatggac tacatgcagc
     8701 agagtgggct ttagggggga ttttttcaga aaaagattat gtctcactgc ttgaagaaca
     8761 gttaaagaca atcgctgaga tcaaagagct atcagttgaa gaagttaaaa aaaatactta
     8821 tcaaaacttg gaacgtttca ttagattagg taaataaaaa agagatgtga cacaactagt
     8881 gtcgcacctc tttttggctt tctttgtata aaagtaaact gtttgattaa taaatgaatc
     8941 ttagtaccaa ccgtttgctt cccagaatgc tttcgccgct tcccaagaac cataacgtga
     9001 agtaacatat tgttctgcta ctctttcttg gtttgcagca gagtagtcac catttaagta
     9061 agaagaatct aattggtaac gtccgatgta acgtccgttc gttgctgtgt aagaaccgct
     9121 tgattctttt tgtgcgatcc attcttttgc agagcttgtg cttgctggag tagcagcagt
     9181 ttctgttaca gcaggagcag gttgtgtttc agcaacaggc gctgcttgtt tttccgtttg
     9241 aaccgtttct tgtacaacag gttgttcagc ttggactggt gcttcttgaa cggctgcttg
     9301 aggttcagca gctgcttgtg cagagccttc tgttgggatt gttaattgtt gtcctgagta
//
LOCUS       DAZULO010000101         9280 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000101 DAZULO010000000
VERSION     DAZULO010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.103"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACG8OA_002417"
     CDS             <1..435
                     /locus_tag="ACG8OA_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFS4977162.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACG8OA_002418"
     tRNA            complement(759..842)
                     /locus_tag="ACG8OA_002418"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACG8OA_002419"
     CDS             1370..1870
                     /locus_tag="ACG8OA_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4977163.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="ACG8OA_002420"
     CDS             complement(2060..2404)
                     /locus_tag="ACG8OA_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFS4977164.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="ACG8OA_002421"
     CDS             complement(2558..3478)
                     /locus_tag="ACG8OA_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4977165.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="ACG8OA_002422"
     CDS             3665..4156
                     /locus_tag="ACG8OA_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFS4977166.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="ACG8OA_002423"
     CDS             complement(4180..6555)
                     /locus_tag="ACG8OA_002423"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFS4977167.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="ACG8OA_002424"
     CDS             6881..7507
                     /locus_tag="ACG8OA_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4977168.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="ACG8OA_002425"
     CDS             7530..8234
                     /locus_tag="ACG8OA_002425"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4977169.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="ACG8OA_002426"
     CDS             8426..9259
                     /locus_tag="ACG8OA_002426"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFS4977170.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2828 a         1791 c         1651 g         3010 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct
//
LOCUS       DAZULO010000102         9038 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000102 DAZULO010000000
VERSION     DAZULO010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.104"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1187
                     /locus_tag="ACG8OA_002427"
     CDS             <1..1187
                     /locus_tag="ACG8OA_002427"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4977171.1"
                     /translation="RKYLFVCLLIPLTFFVELFTDVSAAIPIIGSYFILKEKKKTNIV
                     LLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGYVVFQIMLDSLFLLMFIWLYRK
                     LAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQVFDHFVFGVLIFLIIQMFVVL
                     FLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQEKFARFRHDYKNLLLSLKEIA
                     NQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKNEYLKSLLIAKLYQANNNKICC
                     KFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQDRELSLAIYRDDKQIEISISN
                     SCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKNPNMFVNYQNDPYQFTTQVIL
                     LF"
     gene            1202..1954
                     /locus_tag="ACG8OA_002428"
     CDS             1202..1954
                     /locus_tag="ACG8OA_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4977172.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(2657..2872)
                     /locus_tag="ACG8OA_002429"
     CDS             complement(2657..2872)
                     /locus_tag="ACG8OA_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFS4977173.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(2891..3127)
                     /locus_tag="ACG8OA_002430"
     CDS             complement(2891..3127)
                     /locus_tag="ACG8OA_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFS4977174.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            3385..5538
                     /locus_tag="ACG8OA_002431"
     CDS             3385..5538
                     /locus_tag="ACG8OA_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFS4977175.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            5507..6922
                     /locus_tag="ACG8OA_002432"
     CDS             5507..6922
                     /locus_tag="ACG8OA_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFS4977176.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            6955..7989
                     /gene="sppA"
                     /locus_tag="ACG8OA_002433"
     CDS             6955..7989
                     /gene="sppA"
                     /locus_tag="ACG8OA_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS4977177.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            7991..8512
                     /locus_tag="ACG8OA_002434"
     CDS             7991..8512
                     /locus_tag="ACG8OA_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFS4977178.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            8509..8895
                     /locus_tag="ACG8OA_002435"
     CDS             8509..8895
                     /locus_tag="ACG8OA_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFS4977179.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT         3219 a         1271 c         1599 g         2949 t
ORIGIN      
        1 aaagaaaata tctttttgta tgtttattga taccactcac tttttttgtt gaattattta
       61 ctgatgtttc cgcagctata cctataatcg gaagttattt tatattaaag gaaaagaaga
      121 aaactaatat agtgctttta aataaattac tattatgtat gcttattgat gatttcagat
      181 ctattattac ttccatggtt atgacgtata tgttttcatt tgaagggata aaagggtatg
      241 gttatgtagt attccagata atgcttgatt ctttgttttt gctgatgttt atttggttat
      301 atagaaaatt agctataaat aggattgttg aacagtccag ttctaaactt actgctatat
      361 tcatgattta tttacttaca attggtttat ttgtttctta tgttgctcat caatatcaag
      421 tatttgatca ttttgtgttt ggtgtcttaa tatttttaat cattcaaatg tttgttgttc
      481 tatttttatt tattagaata actattaggc aacgagaaca atatgaacag caattgaaaa
      541 gaaaagaatt ggtttatcta aaaaaatata ctgattcact ggaaaaagat caagaaaaat
      601 ttgccagatt tagacatgac tataaaaatt tattgctgag tttaaaagaa atcgctaatc
      661 aaaaccatga ttcgatattg gaaaaagaaa taaaagaatt agagatctat tctaattctt
      721 atctaaccaa taagtttgaa tatcgttatt taaaaaatat aaaaaatgaa tatttgaaaa
      781 gtttgttgat agcgaagcta taccaagcaa ataacaataa gatttgttgt aaattcgaat
      841 gtccaagtac tattattgaa gttcctgttc caatatttga ctgtattagg gtgcttggaa
      901 ttattctcga taatgcaatt gaagcatcta tagagagtca agatagagaa ctgtctctag
      961 ctatttaccg agatgataaa cagatagaaa tttcgatatc gaatagttgt agagagatac
     1021 tactctctat agatactcta atgaaaaaag gaataacaac aaaaaaggga catcaagggt
     1081 taggtttatc tagtatagag gaaatcaata aaaagaatcc caatatgttt gtgaactatc
     1141 aaaacgatcc gtatcaattt actactcaag ttattttgtt gttttgaggg aaggaggatt
     1201 tatgagttat ccaataatat tatgtgaaga tcaaattata caattaaatc agttagaacg
     1261 aattattgat aatttcattc tttttcacga taaagtattt aagatagtat tgaaaacaca
     1321 aagtccgtta gaagttaaaa aatacctcaa acaattccga ccaaagcaag gaatatattt
     1381 cttagatatt gatttaaatc atgaagttaa cggtatagaa ttagcagaag taatcagaaa
     1441 atatgatgtt caagcaaaaa tcatttttac aactactcat gatgagatgt tacccgtaac
     1501 aataaaaaga agagttgaaa cgttaggatt tgtaacaaaa gatcaaacac tagatgagta
     1561 tcgaaacgag attgttgagt tattgttatt agcgcaagaa aggatagatg caacaaaaca
     1621 ggttacgaat caagcatttg ttttttccat aggttctcaa acatttactt ttgatataaa
     1681 tgacatttat tttgtagaag cttcaaaatt accacataga ttatcattgt gtactaaaga
     1741 tgggcagtac gaattttatg gaagaattag tgagcttgaa aagaaatatc ctatgttaac
     1801 aagaattagt cgagcgtgtt tagtaaatat ctttaacgtt aaagaaatag attttaaaaa
     1861 acgttcacta tattttgatt cagagttggc cagaaacttt actttaggga aagcacaaaa
     1921 aattaaggag aagttgaaag aaagcactgt ttagtggttt tttctttagg gagattgcat
     1981 ttttctaata aaatagattc cttcactagt gcaaattttt ctgaatccta ttaaagttgt
     2041 accaattttt atagctgtta ttagttacgt gcataacagc aatattagtt actggaggaa
     2101 tatttctatg gtctggaacc aaaaaagggc caaatgaata gttcttttct tgtttttatt
     2161 aagtaccata aaggggaatt aaaattaaat gtatggtgga agatgcgaaa gatttggcaa
     2221 attctactaa tttttagaaa gtgtttttca ttttttagtt aaaaaatttg tcctgtcaag
     2281 tgcaatccta tgattgtact tgataggatt tgagataatt actttcctgt ttttataaat
     2341 ttaacttgca ggaacaggct ttaatgcaca tccgacaagt acattaccaa tacctcctgt
     2401 aacggccgaa ataggtcctc ctgcagcacc aactacagcg ctgcaagtcc ttttgtaaaa
     2461 atatctctac cattacattt ccaaagaatt tttatctgac tttaataaaa gataaaaatt
     2521 taatataatt ataaaaacta gcacaagaag ctttagtaaa aagttctgtc cattccaaac
     2581 ttttgaaaag ataaagaaca tacctatgtt cgtaatcgac acaaaccaat cttgaggttt
     2641 gatatttttt aattgtttac gcaccggtcc acatgctgaa tccagcacct aataaagctc
     2701 cacctacacc acaagcagct attgcataag gtcctccggc tgaacaaagt tttactcctg
     2761 ctgttgcccc agaaaaagta gtgcctaaaa ctcccacggc actcccacct tgaatatttt
     2821 tcaactcttc tgaagataat ataaatgttg tttttgtgtt tttaatatgc atactactta
     2881 ttctcctttt ttattttttt cgagcatccc agccagctgc agcacctcca gcgacagctc
     2941 catataaccc tccaacaaca ccgcaagctg gtccgcaaat tgctgttcct aaacctgcac
     3001 cacctactgc ccctgtggta agtccaccta caacttgccc caattttccc caagcattat
     3061 tgcctccacc aacagtttgt gcatttccac aatacttagt tcctttacat tttgcatgtt
     3121 taaacacgct tctttaaata atatttttta cggtgctact gtaacatacg tatttgatta
     3181 acagtaaaaa tcttcctgaa acttatcaag tttattcttg aatagtaaaa tttccaaaat
     3241 aaatttgatt caggaatgaa aaagttagtg ttcaagaact gttttcaaag tttttgtgga
     3301 attatgagac aatattttca ctgtattaaa gctatattga gaatttttct ataaaaattt
     3361 tttagaagga gttgtgttag ataagtgata aaatcatttc cttttgtgca gcaacaagat
     3421 gaaaaagatt gtggtgttgc ttgcctttcg atgattttaa aatattttaa aactgatatc
     3481 ccgattcata aattacggga cctatctggg actgatttag agggtacttc agcatttgga
     3541 ttgaaaaaga catttgaaaa attgtctttt gattgtatgg ctattcaagc agatagtgag
     3601 atctggaaag aaagagatct tatcttacca ttaattgcac atatattggt tgataacagc
     3661 tatatgcact acgttgtaat ttataaaacg aagggaaacg tactatggat tgcagatccc
     3721 gctaatggaa aaaatagaaa aacgattgaa gcgtttcaaa aagaatggac aggtattctt
     3781 ttattaccta tgcctagaga aacttatatt cctattaaag aaaaagtagc tggattaagt
     3841 tcttttttgc ctataatttg gaaacaaaaa ggattagtat tccacatcgt tttagcatcg
     3901 attttcatta cgttttttgg cataggaagc tcatattatt ttcaaggtat tcttgatttt
     3961 ttcatcccta atcaagcacg ttcaacttta aatataattt ccattggctt gatcgctgtc
     4021 tatttatttc gggtattgtt tgaatatagt cgtagttatc ttcttattat tttaggacag
     4081 cgaatgagta tggcaattat gcttcagtat ttcaaacatg tgttaacttt accaatgaat
     4141 ttttttgcta cgagaaaatc aggggaaatt atttctcgtt ttcttgatgc cagtaagatt
     4201 attgatgctt tagcaagtgc tacgttatca atatttttag atattggtat ggtgttgctt
     4261 gttggggtta cgttagctat tcagaatagt acgttgtttt taatcactct atgctctttg
     4321 cctttttatg tagttgcaat attcgctttc gtgaaaagct atgaaaaatc gaacagggaa
     4381 gaaatggcag ccggtgcagc tgtaaattct agtatcatag aaagtcttaa aggaatagaa
     4441 acaattaaag catatagcgg tgaagagaaa gtatataacc gtatagacca tgaatttgtt
     4501 aaattaatga aaaaatcatt tcgtacggtt acgcttgata atattcaaca aggaattaaa
     4561 catttgattc aattaattag cagtgcatta attttatggc taggttctta ttttgttatg
     4621 gataatagaa ttagtctagg tcaacttatc acatacaatg ctttactagt atttttgaca
     4681 gatcctttac aaaacattat taatttacaa gtgaaaatgc aaacggctca agttgcaaat
     4741 aatcgtttaa atgaaatttt cgcgattgat tcagaaaaat cgaaccagtc ttttgaatat
     4801 atgctttctg aacaattatt tcaacaagat atcactgtaa aaaacgtttc attctcgtat
     4861 agcatgaagg ccccaacgtt gaacgaaatt tcatgtacaa ttccttcgca cagtaaaacg
     4921 gctatagttg gagttagtgg ttctggaaaa tcaactcttg caaagttatt tgttcgtttt
     4981 tatgaagcat cagaaggatc gatccattat ggaaacatta atcacttaga cattcctttt
     5041 cagatattac gcgatcgagt gacatatttg cctcaagagt catttttctt tagtggaaca
     5101 atcattgaga atcttatatt cggtttagat agcccaccaa gtttcgaaca aataatagaa
     5161 gtttgtgaga tagtccaact aaatgaattt attaatcaac agccattacg ttacgagaca
     5221 ttgttggaag aaggtggaaa taatctttca ggagggcaac gacaacgatt agccttagca
     5281 cgggctttat taaaaaatcc ggacatatta attttagatg aagcaactag tggattagat
     5341 accttgttag aacatgcaat tatgaattat ctcctcaatt taaaagaaaa aacgattatt
     5401 tttattgcgc atcatttacc aatagcaaaa gcatgtgatc aaattttagt tctccatgaa
     5461 gggcatttgg tagaaaaagg aacgcacgat gaacttcgtt ttgccgatgg tatctatcaa
     5521 aaattatggg aaatctagga gagggaaaca atttatgcag aataataatt ggttcgaaca
     5581 ttctagtatc tatactaaac aacatcatag cttctaccgt tgggtgttat accctgtaat
     5641 tcttttttta tgtattattg gattgttttt aacctttgct aaaaaagagg ttgttattcg
     5701 gacaacggct caattgaacc ccgaaaaaat agagaagtta caagtgccat tagaagctaa
     5761 aataatggaa aacttcctaa aagaaaatca atttgttcat aaggacgatg tgttggttag
     5821 attggattgt tctttaatag agaatgaaaa ggctcagata gaacaagaaa accaacgtat
     5881 tactcaacaa ataaagatgg ctcagctatt tattgaaagt ataagtaaag gaaaaaattt
     5941 gttttcaacg gatgacagtt ttggctacag taatcaatta aagagcatgt tgtcagaaaa
     6001 agaatcactc cgctacgctt tgaagcaaag tgaattaaat gatcaaaagc aattagaagt
     6061 atacgaaaag acaaaaagac aactagaaaa acaaattgag agttcagata gtaaattaca
     6121 agaatggcaa caagtacagg tagcttggag taataatcaa tcattaaaag atttttcaaa
     6181 agaaatgatg gcaaactatg agaattggca agaacaacta aataatgttt ctgatgatca
     6241 aaaaaatcaa gtgaaactga caatttcagc aagcataaat gaacaaattg agcaactaaa
     6301 aaaagaagta gaacagtatc agtcagaaaa agctaaatta gttaaaccaa ctacttctga
     6361 gaatgacaga attagtcaaa cggaaaaagg aaagcaagag ctagaacaag caataacaac
     6421 tgtaaaacaa acagttacag agctaaatga aaaacaagag aagaaccagt cagttatcaa
     6481 aagtttggac gatcaacttt caaaaggaat actaaaagct cctgtatctg gaacggttca
     6541 tttaaatgaa gaaacaaagg gacaagcaga actagccaaa ggaacagtgc ttgcagaaat
     6601 ttatccgatc cacgaaaaat caaaaatgaa atttacagca cttttacctg cgaatgaaag
     6661 tacctatatt aaagaaggga tgaaggttca ttttaaattg gatcaaaaag gtggggctcc
     6721 gattacgatc gacggaactt tagatgaaat ttcagaaaat tctactgcca ctgagaaagg
     6781 tgttttttat gcagtgaaag gaacgttaaa aacaactcaa tcgtttccgt ttcgttatgg
     6841 attaactgga gagttgtctt taattgtagg agaaaaaaca tattggcaac aaattgtcga
     6901 tgtgtttcta aataaaaaat aattattttt actaagaata taggaggagt ttatatgaat
     6961 aaaagaagat ggattgctgt gggagtagcg tgcggattat tgttactatc cattatcgtc
     7021 gcgctaattc caggaaaaga taaagaggaa gcatcaaata caaccctaac aggaataaat
     7081 aagatttttt acggatctaa cgaaatcaca gaagaaacgt tagaagaagg cgcatcaaat
     7141 aaaaagattg tcaaactatc cgtcaatgga gttattgcag atacaggtga aagcaatctt
     7201 tttagtaggg aacaatataa ccatcagaat tttttaacac agctaaagaa aattcaagaa
     7261 gacaaagcgg ttaagggagt gttattagaa gtaaattcac ctgggggagg aatatatgaa
     7321 agtgctgaga ttgctaaaga aatggcaaaa attaaaaagc tagatattcc tatctatact
     7381 gcatttaaaa atacagcggc aagtggaggg tactacattt ctgctggttc agataaaata
     7441 tttgctacgg aagaaacaac aactggatca attggtgtca ttatttctgg tttaaattat
     7501 tctggcttat tagaaaaact tggcgtaact gatgcgacgt ataaaagtgg tgcattaaaa
     7561 gatatgatgt cacctcaaca caaaccttca gaggaagaga ataaagttat acaggaattt
     7621 gtgatgagtg cttatgatcg ttttgtcaat gttgtagcaa aaggaagaaa tatggatact
     7681 aatgcagtga aagaacttgc ggatggaaga atttatgatg gtaaccaagc agtagaaaat
     7741 gggttggtgg atcaaattgg ttactctgaa gatgcattag attccctaaa aaaagagaag
     7801 aaattaactg atgcgacggt tatagaatat aaaaatgaca caactggctt tgcttcttct
     7861 tggttaggta ataagattgc tgaatggcaa gggctaaagg caactacatc agatcgtgta
     7921 atttcagttt ttgaaaaact tggattttct gatactccaa aaccaatgta ttactatgga
     7981 gggctataaa atgaataaga agagtggcat tgaaactttg cctaattatg tatttgcggg
     8041 attttggtta cgattttttg cttttcttat tgatttaatt tgtattagca tcatcaccca
     8101 tgctactata ggtctctttt acaaaatcat gggactggaa tctgacaaat ccattattac
     8161 tttttatggt atagcgtcat tagctattta tttaacctat tttgtacttt tgactaagct
     8221 gaattcagga caaacaattg ggaaaatgat ttttgggata agagtagtga gctttgagga
     8281 aaaagagcta agttggcaaa ctatctttat tcgtgagtgt atatgtaggt ttatattaaa
     8341 aaattttgta tttcaaattg ggtatttggt ggctgctttc acgcccaaga aacaacatat
     8401 tggggattat ttttctaaca cgtcagtggt tgttattcgt tcattagaag ctattaacta
     8461 tggtcaaaaa atttctaatt ctaaaacact taatctggag agaattaaat gaataagaga
     8521 aaaacagagt ttattttgat tttaattagt ggaataacag ggattttatg tagtctttta
     8581 gcattgatgg gtggtatgtt tctatcttct attgattcaa ctactttaaa tactacagaa
     8641 ctaaccagta aggatataga tatgttagtc aatctaagcg ggaatgttgt tttatttgga
     8701 ggaattaccc tagttttttc gataatgatt ttcataatag gttttttagt aagaaaaaat
     8761 caaaaaacag tggtctatgg tattagttta ctcattttat ctattattcc attttttctt
     8821 ttaacattat tgtgggtaat tcctggagtt ttaggtatta ttgcgggatt gatgcttcta
     8881 ataagaaaat cttaattagg caatgtgtag acaacgtgaa acgattaatc aattaaaagc
     8941 taaatacgtt ttgagaaatg ataacttgta gtcgatggaa gacatgtaga taaaaaaact
     9001 acgtgtcttt tatttattct aattttttcg aaaaaaag
//
LOCUS       DAZULO010000103         8494 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000103 DAZULO010000000
VERSION     DAZULO010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.105"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACG8OA_002436"
     CDS             complement(<1..149)
                     /locus_tag="ACG8OA_002436"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4977180.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACG8OA_002437"
     CDS             complement(593..1714)
                     /locus_tag="ACG8OA_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFS4977181.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACG8OA_002438"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACG8OA_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFS4977182.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACG8OA_002439"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACG8OA_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFS4977183.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACG8OA_002440"
     CDS             complement(4304..5215)
                     /locus_tag="ACG8OA_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4977184.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACG8OA_002441"
     CDS             complement(5232..6239)
                     /locus_tag="ACG8OA_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFS4977185.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACG8OA_002442"
     CDS             complement(6236..8365)
                     /locus_tag="ACG8OA_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4977186.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACG8OA_002443"
     CDS             complement(8370..8480)
                     /locus_tag="ACG8OA_002443"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977187.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DAZULO010000104         8301 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000104 DAZULO010000000
VERSION     DAZULO010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.106"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..382
                     /locus_tag="ACG8OA_002444"
     CDS             <1..382
                     /locus_tag="ACG8OA_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFS4977188.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNLLTIPGAMSPTEIFKMFEMGADI
                     VKVFPANCLGPEYFNQVQAPLGSLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKS
                     AVHQLNYERLMELAESFIDSLRVE"
     gene            complement(776..1783)
                     /locus_tag="ACG8OA_002445"
     CDS             complement(776..1783)
                     /locus_tag="ACG8OA_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4977189.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            2250..3656
                     /gene="lacG"
                     /locus_tag="ACG8OA_002446"
     CDS             2250..3656
                     /gene="lacG"
                     /locus_tag="ACG8OA_002446"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFS4977190.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            3679..4002
                     /locus_tag="ACG8OA_002447"
     CDS             3679..4002
                     /locus_tag="ACG8OA_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4977191.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            4013..5695
                     /locus_tag="ACG8OA_002448"
     CDS             4013..5695
                     /locus_tag="ACG8OA_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFS4977192.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
     gene            5901..6859
                     /locus_tag="ACG8OA_002449"
                     /pseudo
     CDS             5901..6859
                     /locus_tag="ACG8OA_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6878..7105
                     /locus_tag="ACG8OA_002450"
     CDS             6878..7105
                     /locus_tag="ACG8OA_002450"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HFS4977193.1"
                     /translation="MYQVFEEMGYPIKTVTRKLEVIKADDTISDLLDVEEGDPMFYFH
                     TQGFSLERLPVEYSISKYRGDINYFVFELAQ"
     gene            7320..8072
                     /locus_tag="ACG8OA_002451"
     CDS             7320..8072
                     /locus_tag="ACG8OA_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4977194.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
BASE COUNT         2700 a         1268 c         1691 g         2642 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccttcta acaattcctg gggccatgtc gcccacagag atattcaaaa tgtttgagat
      121 gggtgctgat attgttaagg tgttccccgc caactgtctt ggaccggaat atttcaatca
      181 agtgcaagcc cctttaggaa gtttaccttt gatggctgta ggaggggtag atcagactaa
      241 tgcacaaaac tatttgaaca atggagcctc ttatgtagga attggttcaa aattttttga
      301 aaaaagtgct gttcaccaac taaattacga gaggctaatg gagttggctg aatcttttat
      361 agatagttta agagttgaat agaacccatt tgcggaatac aacttgtttt ataatatggc
      421 gttatctgta taaaacatag tatcacagtg aatgagattg aactggtaga ttgatttttc
      481 gaccagttct ttttgcgcgc tctgatgctt gatatattcc caacgagata aaaaatcccc
      541 caagatacta agatgaagcg tttatccaac ctcatactta gataaaaagg aggaaatgtt
      601 ctgaatgata aacaaaaatt agcgcgcaca atttggcgat gaaaaaatca tattgtattt
      661 acgtcaaaat atcaccatca aattatttat ggaaaacata ataggcataa acccgattgc
      721 tcggctacta tgtggacaca attatagttt taaagataag tttcaattac ttattttaca
      781 cctcttcttt agggataaaa cttttcctaa cggtcaattt tgcgccgatt aatgttttct
      841 ttgatatgtc ccgcgaatat accagttgat cacttaataa atctacacag gttttagcta
      901 tctcttcgat atctatctta aaagttgtaa gtggtggtgc aacgaatttt gcgatgtcgt
      961 catcattaat acttattact gccatatctt tagggatgtt aatacccaat tcatggcagc
     1021 cctgaagaac gccggcagct atagtgtcag atggtatgaa acatgcctcg ggcatatctt
     1081 catttaactc agctttcatt ttaagagtta attgataccc ttcgtcaaca gtaaatgatc
     1141 cttgggagaa aatatacttt tcattgagta gtccgctctt ttctaaatga tatcggaagg
     1201 catattcacg gctatcaatc tcttctttac ccgtttctag gttaaagaaa tttcctccaa
     1261 taaatccaat ttttgtgaaa ccttcattaa taaagtagtt aattgcttgt ctggccattt
     1321 cgtggaaatc aggttctaca gtatcgaagt gttcaggtaa tgggtttatt cctcggatta
     1381 cccccttata ccctttttga tttaagtagt acaattcctt ttttgtcaat tgtcctattg
     1441 cgataaatcc agtaatatcg tcaggaatgc tttttacacc atcgttttta gagatgaaaa
     1501 tcaaatctag attagctttt ttactataat ttttcaaatc atctttgaca tgttgatagt
     1561 agacatcctt ttttaactgt tcctctgaga tccaaaaaag aatggcaatc ttgtacagag
     1621 tagtcttaat ttttgtcaga tcatatccta aagatttggc tgtaacgatt attttgtttt
     1681 tagtatcaga tgtaatagag agtgactcat cacctttaaa aagtcttgat atagtagccg
     1741 ttgagtaacc agattttttt gcaatatctc taatagtaac cattgttcat cctcctaggg
     1801 atataataat ccttttcttt gttaaagtaa atcgaaactg ctcttttagt aacttaaatt
     1861 actttttcta gcaggaaacg aaactctgat atcagcagag agcctaatcc attaacagag
     1921 aagattgaat cgataatgga tagccacttg gagaatgatt agtaggtggg attgatttta
     1981 gtgggaatca gtccgttttc ttgtttcgtg taaattaaat tagtaaaaag ataaatttat
     2041 tttacaaaaa aacgtttaca aaacggtttt tgaatggtat atttatttta catcaaggga
     2101 tataagtgct tgtatatcgc aataaaatag atattcgagg tcggtttttc gttgtgtaaa
     2161 aaaatttact tatacatgtt aaaattatgc tgaggtgatt tatggattat tggcatattg
     2221 atcaaactaa ttttcaagga gagattaaaa tgaattataa aactttacca gaagacttta
     2281 ttatgggagg agctacggca gcttatcaag cagaaggctc tacaaaagaa ggtggaaaag
     2341 ggcaagtagc gtgggatact ttccttgtag aaaagggacg ctatactgct gatccagcta
     2401 gtgattttta taatcaatat ccagttgatg ctgaattatg caaaaaattt aaaattgata
     2461 gtattaggat gtctattgca tggagccgta tttttccaaa aggctatggg gaagtgaacc
     2521 aaacaggagt ggaatattat cataaggtat ttgctgaatt gaataagcat ggtattattc
     2581 cttttgtaac tcttcatcat tttgataccc ctaaagaact atttgataat ggagattttt
     2641 taaatcgaga aaatgttgaa catttcgtaa actatgcaca gttctgtttc gatgaatttt
     2701 ctgaggttca ttattggagt acatttaatg agatttatcc agttgcttca aatcaatatt
     2761 tgatcggatc gtttcctcca gaagttcaat ttgatataga gaagactgta acttgtttac
     2821 acaacatgat gtatgcgcac gcaaagacag taaacatttt taaagatggt ggatataaag
     2881 gggaaattgg tgttgttcac tcgctagaga caaaatatcc agcaagtgac aaagaagaag
     2941 ataagcatgc tgcgaaatta gatgatgcgt tatcaattaa gttcttatta gatgctactt
     3001 atttagggta ttactcagat gaaacaatgg cacatattaa tgagatttta gcagcaaatg
     3061 ggcaatctgc agatattgat tctaatgatt ttagagagat gaaaaaagca gcaaatcgaa
     3121 atgattttct tggtatcaat aactatggta gccattgggt taaagagtac aaaggagaaa
     3181 gcacacttca ctacaacact accggagaaa aaggtagcca agtctattgc attaaaggga
     3241 ttggcgagag aatcgtgcgt gaggatcttc cgaaaaccga ttgggattgg atcatttatc
     3301 caaaagggtt atatgattta cttgtacgaa tccaagacga atatccaaat tataataaaa
     3361 tatacattac tgaaaacggg ctagggtata aggataattt tgaagatggg attattctag
     3421 atgatgatcg aatcgattat ttacgccagc attttaatgc gatttccgat gctatcaaaa
     3481 tgggagttaa tgtaaagggg tatttcattt ggtcattaat ggatgttttc tcttgggtta
     3541 atggatacac taagcgttat ggactattct atgtagattt cgatacacaa aaaagatatc
     3601 cgaaagagtc tgcatattgg tataaatttg ttagcgaaac aaaaacgata atataaaaat
     3661 agttaggagg gcaaggaaat ggataggaaa gaattgcaat tattaggatt tgaaattgtt
     3721 gcttatgcag gagatgccag aagttcatta ttaacagtgt tgaaggaagt gagagaaggt
     3781 aattttgata atgttgaacg attattagcg gatgctgatg aaaatttaac cttagcgcat
     3841 aactcacaaa cgaaatgttt agctgaagaa gcgtcaggga acgaaatgga agtaggcgta
     3901 atttttattc atgggcaaga ccatttaatg acaacgattc tattaagaga acttatcaat
     3961 gattttgttg aattgtacaa agaaaaattt gctaaagcat aggagaagaa aaatgaatac
     4021 gattattaag caaattgaga agggaaaacc gttttttgaa aaaatctcac gtaatattta
     4081 tttaggggca attcgcgatg gctttttaac tgctatgcca gctattctat tttcaagtat
     4141 ttttattatg attgcggctg ttccagaagt atttaacgtg acgttaccag aggcattctc
     4201 gacgtggctt tggaaagttt atggttattc aatgggatta gttggtcttt tggttgttgc
     4261 tacaacagct cgatgtttag ctaattcaat gaaccgtcaa ttatctgatt caggtaagca
     4321 gattaatgaa gtttctgtta tgttagcgtc aatttgtggt tttgtaatga tggcagctga
     4381 tcaaattgat ggtgggatag ctactgatta tttagggaca aagggattat tagcagcttt
     4441 cgtttctgcg tttgttactg taaatgtgta taaattctgt gttaaaagag atttgacgat
     4501 ccgtatgcca aaagaagttc ccggaacaat cacacagatg tttagagata ttatcccctt
     4561 ttcaattgca gtatttattg ccgttttgat tgatacggga gttcgtcaat tttttggaac
     4621 atcatttgca gcaggtgtaa ttcaattatt gcagccatta tttagtgctg ctgatggata
     4681 tgtcggtatt gcaatcattt ggggagccat ggcaatgttc tggtttgttg gtgttcatgg
     4741 accatcgatt gttgaaccag cgattgctgc aattatttat gcgaacgtag aaactaactt
     4801 tgaattattt aaaaacggtg cacaagcctc aaaagtatta acagttggtc taggaaactt
     4861 tgttggaacg atgggtggta ctggagcgac actagttgtt ccttttctgt tcatgttgtt
     4921 cgctaaatca aaacaattga aagcagttgg taaagcttca tttattccag tttcgtttgc
     4981 ggtaaatgaa ccgttactat ttgctactcc tattattttg aatccgtact ttctaattcc
     5041 atttttatta gcaccaatcg ctaacgtatg gatattcaaa ctttttgtcg atgtcttggg
     5101 gatgaatagc tttatatccg tattgccttg ggcgacgccc gcaccgattg gcttggtttt
     5161 aggaaccggg tttagtgtgt tggcgattgt attagcggta gtattgattg ttgtcgatgc
     5221 gattatttat ttaccttttg ttaaggccta tgattctgaa ttagtagaac aagagttaaa
     5281 aaacattgct gcagaagaag taaaagaaga attggtcgaa gaaatccaag aagttggtgc
     5341 aactgcggca gttatagttg atgaagcaaa agcatcggtt ggtgaaggaa agcgcgtttt
     5401 agtactttgt gctggtgccg gaacgagtgc aatgttggct aatgcattag aagagggcgc
     5461 aaaagagaca ggtgttaatc tttctgctaa agcaggagct tatggttctc attatgatat
     5521 gatgaaaaat tttgatttaa tcgttctagc acctcaagtg aattcctatt tggatgatct
     5581 ccgtaaagat gcagacaaat tagggataaa agtaattgca actaaaggtg cagaatatat
     5641 caatttaaca cgtgatccta aaggagcagt tgattttgta ttgaagcaat tttagagctt
     5701 gcttagatta ataaagcgct agccaactta gctagcgttt tatcttaaac acaatggtaa
     5761 gcgctatctt tttttgctgt ttaaatttcg ggtattcttt ttgaggcgtc gattgtaaaa
     5821 ttaagctaga caactaaaaa gatcatttgt gctacactca aaatagttcc gaccaaagaa
     5881 ttaataagga gtgagtacaa atgacctaca cccatcttac aacagacgaa ctagtaatca
     5941 tagagtctta tttcaatatc gagaaacctg tcgctcttgt cgcacaaagt ttaaatcgct
     6001 ctagacaaac catttataat gtttattcgt ttctcaaaca aggaaaaaag tgctagatta
     6061 ttaccgtcaa tacaaacaaa ataaatctcg ttgtggacga cgttcaaccg ttttatctga
     6121 tgaacaatca acatatgttc aagaaaaggt agctcaaggg tggtcacccg atgtgattat
     6181 cggccgtggc gagtttccaa ttgattgttc tgttcgtaca ctctatcgga tgtttaaaaa
     6241 aggccttttt gacacacagt cgttacctat gaaggggaaa cgtaaaccga ataatcatca
     6301 agaaaagcgt ggaaaacagg cctttcgcag aacgattcat gaacggaaca gtctctatcc
     6361 acacctgact aaagaatttg gtcatctcga aggcgacacc attgtgggag ttaaacacaa
     6421 gagcgctgtt attacattgg ttgagtgttt atccaaggtt attattactt tgaaaccgac
     6481 aggcagacga gccgaagata tcgaaaacag gttaaatgat tggttcaaat ccatgcctaa
     6541 acacttgttt aaatccatga catttgattg tggaaaagaa ttttccaact ggcgatctat
     6601 aagtaactcg aacgatattg ccatcttttt cgcagatcca ggaacacctt cgcaaagagg
     6661 gctaaatgag cactcaaacg gcttgcttag acgagatggc ttacctaaaa caatggactt
     6721 tagggacatg gatgaaacct ttattcaatc cgttgcctcg aaaagaaacc atattccaag
     6781 aaaatcatta aattatcgaa caccattgga agtatttttg agctgcatag ataaagacat
     6841 attgtctagc ttaatttgac aaatcagaaa agaaaaaatg tatcaagttt ttgaagaaat
     6901 gggttatcca attaaaacgg tcaccagaaa actagaagta atcaaagcgg atgatacgat
     6961 cagcgatttg ttagatgtgg aagaaggaga tccaatgttc tatttccata cacagggttt
     7021 ttcgctagaa cgtctgcccg ttgagtattc gatttcgaaa tatcgaggag acattaacta
     7081 ctttgttttt gaacttgcac aataacttca gattaaataa aatgatttat taaagataaa
     7141 agtcgaatga acaaggctgt cactatgaca gtcatgctcg ttcggtttta ttttttagaa
     7201 tagcagcttg caaattctga aaaattttgc tttccttcct gcagaaggct tcttaagaaa
     7261 acttgttcta gcacttttct taagaatttg atagtcttaa aaagaaaaga ggcgatgaaa
     7321 tgttaaaaac ggatcgattc caagcgatca tgtcgttatt agaggttaac ggtacagtac
     7381 gagtcagcga tattatggag aagttttcgg tttcggatat gacagtccga cgtgatttgg
     7441 acgaaatgga gaaacaagga ttattagaac gcgtccatgg cggagcgaaa ttgaaggatt
     7501 tttatcggca tgtggagctt tctcatagag aaaaacagat tatgctgcaa gaagaaaagc
     7561 agcggattgt ggaaaaggcg aatgaaatga tcaatgatgg tgatacgata tttcttggac
     7621 caggaactac aatcgagctc atgtcaaaaa tcattcaagg ggattcactg cggattgtta
     7681 caaactgttt gcctgttttt gaagaattaa ataagcgaaa agggaatcga aaaatctatc
     7741 tattaggtgg cgagatgcgg gaaacgactc aatgctttgt tggtgaaatc acgaataaat
     7801 cgctacagga tatgaatttt cataaagcat ttttcagctg taatgcttta aatgaacaga
     7861 aaattatgac ttcgactttt gaagagggcc aaactcaaca gatcgctctg aataattctg
     7921 ttgaacgctt tttattgatt gatcattcga aaattgggaa aagagatttt tcagtatatt
     7981 ataatctgaa tgatgtaaca gtagttataa cggatgatga tgaacaaaag cactatgaaa
     8041 aaatcgaaaa agaagaaaaa tatgttgtgt aaatgagaaa ctgccaaact ctagataatt
     8101 agaacttggt agttttttgg tagccttgaa tttaagcgaa ttatgttatt tttagggtgc
     8161 atcattacta attcctccga atacttgaat acaccatttg aaaaaagata gtcgattttt
     8221 ttgggatgtt atgtttatgt gaaggaaaaa atagcagtat caatcaccgc tttattaagg
     8281 aggaaaaggg atatcaaaac a
//
LOCUS       DAZULO010000105         8210 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000105 DAZULO010000000
VERSION     DAZULO010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.107"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACG8OA_002452"
     CDS             476..1339
                     /locus_tag="ACG8OA_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4977195.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACG8OA_002453"
     CDS             complement(1468..3090)
                     /locus_tag="ACG8OA_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4977196.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACG8OA_002454"
     CDS             3339..3860
                     /locus_tag="ACG8OA_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS4977197.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8OA_002455"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8OA_002455"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFS4977198.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACG8OA_002456"
     CDS             5941..6759
                     /locus_tag="ACG8OA_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFS4977199.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACG8OA_002457"
     CDS             complement(6862..7059)
                     /locus_tag="ACG8OA_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977200.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACG8OA_002458"
     CDS             7228..7806
                     /locus_tag="ACG8OA_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS4977201.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACG8OA_002459"
     CDS             7901..8038
                     /locus_tag="ACG8OA_002459"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977202.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2461 a         1480 c         1619 g         2650 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa
//
LOCUS       DAZULO010000106         8129 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000106 DAZULO010000000
VERSION     DAZULO010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.108"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            384..572
                     /locus_tag="ACG8OA_002460"
     CDS             384..572
                     /locus_tag="ACG8OA_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFS4977203.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            650..1663
                     /locus_tag="ACG8OA_002461"
     CDS             650..1663
                     /locus_tag="ACG8OA_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4977204.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1730..2764)
                     /locus_tag="ACG8OA_002462"
     CDS             complement(1730..2764)
                     /locus_tag="ACG8OA_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4977205.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2879..5104
                     /locus_tag="ACG8OA_002463"
     CDS             2879..5104
                     /locus_tag="ACG8OA_002463"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS4977206.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5122..7005
                     /locus_tag="ACG8OA_002464"
     CDS             5122..7005
                     /locus_tag="ACG8OA_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFS4977207.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6986..7960
                     /locus_tag="ACG8OA_002465"
     CDS             6986..7960
                     /locus_tag="ACG8OA_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFS4977208.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2631 a         1478 c         1712 g         2308 t
ORIGIN      
        1 tagttagtct acattatttt ggcgatttgc caataaggac tgttgagttg tgcaaaatca
       61 aatctgggtc agaatgaacg tcattctgtc tcagattttt cgttatttca atttgattaa
      121 ctgattgatc aaaaagattt ttattttcat attctcaaat gattcaacac taaatataca
      181 tgtttgacgc ggaaattatt attcgatttt ggcggcatca ggggggatga cgacgtagca
      241 gacccattat tttactcaag aaaatgtccg tgcctattat ttagaatacg atagtgaacg
      301 tgctgggagt tttgttcttt tagagaaagt ctctgaagat aaattagttg tcttagattt
      361 tgactgtaac gaaaaatggc gaaatggaag agcaccagca agtgattgac cggatctacg
      421 aagcgacacg atatctgcct ttggatcgtt tgtgcttaag tcctcaatgt ggatttgctt
      481 caacagaaga agaaaatgtt ttgtttgaag aacaatggaa aaaatttctt ttgtcaaaag
      541 cattgcagaa gaagtttggg gaaaatcatt aaatcatcta ggagagcttg ataggaaata
      601 acactatggt ataatcagaa tgcttaatac taactactga gggagcctta tgaaaaagaa
      661 aattcttaaa tttatcggat ggttcttcgg aatcattctt gggatcgcgt ttgcaatctt
      721 tcttgccttc cagctttccc ccagacctgg agcattcttg atcaaccata tgttttcaaa
      781 tactgttcaa atcactgata aaaaaacgtt cgatcaagca gaaaaaaacg tggcgaaaaa
      841 aacagatttg acgtatgagt ccaagtataa agagaatact tatgacatct actatccaaa
      901 aaacagcaag gggcctgttc ctgttctatt ttgggtacat ggtggaggat tcgtcggcgg
      961 agataaatcg ggtgtcaaag aatttgcgac aaaattagtt tcagacgcca acatcgcagt
     1021 ggttgcgatg aattatgaat tagcgcctga ttcagaatac cctaatcaag ttcttcaagt
     1081 gaatgaactg atcaaagatt tattagctga agaaaaagac gacaaattgc tagacatgga
     1141 acaattattt tttggtggag atagtgcagg ttcacaaatc gcattgcaat atgcagctat
     1201 acaaacgaat gatgcgtatg ctaaacaaat gaaaatcaaa cagctacttc caaaagatag
     1261 tctgaaagga accatttctt attggggacc tgttgatttg aagctaacag cacagcagca
     1321 ttcggatgat cgatttatga agtttttcgt caaaaccgtt gcgtggtcgt tgattggaac
     1381 aaaagattgg aaaacgagtg agcaattgaa agaagctagt gtggctgacc atgtttctaa
     1441 ggaatttccg ccaacgtata taactgacgg aaatgcatac tcattccagg agcaaggtca
     1501 agcattagaa aatcggttga cagaattaaa ggtacccgtg caatccttgt tttatacaga
     1561 tacaaaaaaa gagattaccc atgaatacca atttgattac acgctaaaag aatcgcaaaa
     1621 ttgttaccag cagacattgg attttgtgaa taagtataag taagaggttg tgaaaaagcc
     1681 gtttcgtttt gagtatgaag aaacgaagcg gcttttcaca acctctcttt caataccttt
     1741 ttgttcttgg gataaaactt tccctaacaa tcaatcgtgc aggataagtc aaaatgattg
     1801 gttcgtcccg tataccgtca attcgctcca atgccattcg aacagctgct tgcccaattg
     1861 atttcgcatt gatttgaaca ctggtcaagc caggggagag gtttgcgatt gtctcgatat
     1921 tatcaaagct tgctacaaca atctcttctc caatttggat atttttttgt ttcaatgccc
     1981 gatagacacc aattgccatt ggatcgctcc cgacaagaat ggcagtagga agaggggatt
     2041 cattttctaa tagattagtc gcaagtacct caccttgttt ttctgtccag ccacctagat
     2101 gataagacat atatttttct aagtgatgat cgatcataaa atgctgatag gcacgcatcc
     2161 tcttttcatt tatagaaatc tgcttcccac cgaattcatc catatcgact ttatatcccc
     2221 cgatataggc tattctttta tgaccatttt ctaagaacaa ttccagtacg gttttagtca
     2281 tccgttcaag atctgcatac accatatcga cctcttgctg gatgtcaggg ttatccacga
     2341 cgactatttg catgttttgt tttaacaagg cggtaatcga ttctttcgta acagtcccaa
     2401 caacgatcac cgcacctaac tgatctagat cttcccattg tttaggatta tcagatagat
     2461 tatagattcg attcatccct aaatgcagac gtcgagcttc ttcttccatc cctactcgca
     2521 aatgtttgta atagatatcc tgttcttcgc tcgctttgcc gatcgtagag atcacaccaa
     2581 tcgaaggcat tctctttttg atatatttac gagtactgat gtcataatcc aacgcagcag
     2641 ctgcctctac tatccgttgc cgagtagtct gagccacatg gatcgtctga tcgttattca
     2701 acacacggga aactgtagca ggagagacat ttgcctttcg tgcgacatct ctaattccaa
     2761 ccatttttac acactcctca aaaaaataaa aaatttttat tgacaaaatt gtttagtaaa
     2821 cagtatacag gaattataaa aaaaagtaaa cggtttccgt tttgttggga gtgaaatgat
     2881 ggatattacg catagaaaaa cagataaaaa tttcatctta tttgatgaaa agagccgagt
     2941 cttccattta cgaaatacat ttctttctta tctgataaag atagaagaaa gcaatgtatt
     3001 ggcgcatatt tattttggca aaccagtcaa gcagtacaaa gacaataaaa attatccccg
     3061 aagagatcgt ggattttcag gaaatgttcc cttaaatcct gaccggtctc tgtcgaaaga
     3121 tacactaccg caagaatact cttctcatgg cagcatggac tttcgaacgc ccgcatcgat
     3181 cattcagcgc aagaatggat cagacttact ggatctgaga tatgacagcc attatattac
     3241 agatgggaag ccagagatcg aaggcttgcc tcaaacttat gtgatggaca agtcagaggc
     3301 acaaacgttg gtgatttcac ttaaggatag agaaacagcg atttatttcg atttgttcta
     3361 taccatattt actgatagag cagtgatcac acgatctgta aagataagaa atgaaaccgg
     3421 agaaacaatc aaactagaaa aagcggctag tttccagcta gactttgccc atactaggcg
     3481 atttgatgaa gtcattgctt tgccaggagc acatgtcaat gaaagacaga tttcacggca
     3541 atctgtttta tctggaacaa aagtatttga aagccgtcga ggaacgagca gtcaccatat
     3601 gaataacttt attgctttag ttcatcatca tacgacagaa aatactggag aagcgatcgg
     3661 acttcaattt gtttattccg gcaaccattc tttcgaactt gaaaaagatc agatcaatca
     3721 attgagagtc gtcggtggga tcaacagcca tcgtttttcg tgggaattga atgcaggcca
     3781 gtcttttcaa acacctgaaa tgatcctttc ttactcaagt cagggattga acaaaatgag
     3841 tcagatccac catgagttgc tacgtgaaag aatcgcaaga ggtcgtcatc aatttgctga
     3901 acggccgatt ctggtcaata actgggaagc cacttacttt gacttcaata gtgaaaaaat
     3961 caaggcgatc attgatgaag caaaggagct aggcatcgaa atgttcgtat tagatgatgg
     4021 ctggtttgga aaaagagatg cggacaattc ttcattagga gactggttcg agtatgaagg
     4081 caagttgaca aatggattac gtgaaatagc agattatgct catagcaaag gattgaaatt
     4141 tggtttatgg tttgaacctg aaatgatctc ggttgattct gaactgtatc gaacacatcc
     4201 tgattttctt atgcaggagc caggtagaat gccttctgct tccagatcac aacatgtact
     4261 ggattttaca aggttagatg ttcggcagac gattgagaaa caaatgagaa agattttaga
     4321 tacgattcct cttgactaca tcaaatggga tatgaaccgt tcgctttcag atgtctattc
     4381 gattaccctt gacccacaga gacaaggaga agtagcgcat cgttatatgc tcgggcttta
     4441 tgaactactt gaacatttga caactgatta tccggaaatc ttatgggaag gctgttctgg
     4501 tggaggtgga cggtttgatg ccgggtttat ttattatatg ccgcagtcat ggacaagtga
     4561 taacacggat gcagtggagc gtatgaaaat ccaatatgga acaagccttg cttatccgat
     4621 ttcgtccatc acagctcatg tatcagctgt accaaatcat cagaccgaca gatctacttc
     4681 gttgaaaaca agaggagaaa cagctatgag cgctgttttt ggttatgaac tggatttgac
     4741 aaagctgtct cctgaagaaa aaaagcaagt aaaagaccaa attatatcct atcaaacgat
     4801 tcgtccggtg atccagtatg gccattatta tcgtttggcg agtccttttg aagaaaatat
     4861 tgccgcatgg atgtttgtgt ctcctaaaca agatgaagct attgtgtttt tagggaggat
     4921 actagcttca gctcaaccag cgttccatga agtatatctg atggggttag atgatgaggc
     4981 actttatcag gaacagacct cgaagcggat attttcgggg gccgaattga tgacagttgg
     5041 actttacttc cccgattttc aaggtgattt ccaaacagaa ctgcttcatt tcaaaaagtt
     5101 atgagagaga aggaaaaaag tatgaaagca aatataaaat ggttagatga tccacaagtt
     5161 tttagagtga accaatcaaa tgcacatagc gatcatgttt gttatgcaac aagggaagaa
     5221 gtagaaacca catcctccct taaacagtct ttaaatggat catggcgttt ttgttattcg
     5281 ccaaatcctt cggtacgacc aaaaacattt tataaaaaag attttgatgc ctgttctttt
     5341 gatgaaatca ctgttcctag tcatattgaa ttagcaaatt atgacaagat ccattacatc
     5401 aatacgatgt atccgtggga gggacatgta tttcgccgtc cccctttcac gacaagccct
     5461 gagaaagagg cggaagggat cttttcagct gctaaagaca atccggttgg ttcgtatttg
     5521 acgacttttc gattagaaga aggtctaaag ggaaaacaaa tctcgattca ttttgctggt
     5581 gtagaacaag caatgtttat ttggttgaat ggtcatttta tcgggtatgc cgaagattcg
     5641 ttcactccgt ctgaatttga tttaacacct tatttggaag aagaaaatat tctagctgtg
     5701 gaggtttaca aaagaagtac agctgcattc ttggaagatc aggacttttt ccgatttttt
     5761 gggatcttca gagatgtctt cttagttgct cgaggacaaa accatttaga agatctttgg
     5821 atcaaacctc gcttagatga caaatgtcaa aatggcaaac ttgaagtaga tttgtctttt
     5881 gagcaagtag ccaaaaacag tcagctgaat gtagcgatac tagatcctga tgggaaggaa
     5941 atctatcaac agcaatatcc gttaaaagag caaatgacga ttcgcatcga tggatttgaa
     6001 aatgtggcac tgtgggggca ttttcagccc aatttatata agcttgaatt ggagacgata
     6061 gacgaagaaa aaagagtaac ggagtatatt gactattcct tcggttttcg taaaatcgaa
     6121 atcaaagata aaatcatcta cttcaatgga aaacggatga ttatcaacgg ggtaaatcga
     6181 catgagtggc atccgaaaaa aggacgcgcc atcactatag aagatatgga aaaagatttg
     6241 gctatcataa aaaatgcaaa gatcaacggc gtacggacat ctcattaccc caaccagatc
     6301 acttggtatt atttatgcga ccaagcagga atccatgtga tggcggaaac gaacctcgaa
     6361 tcccatggct catggcaaaa aatgggcgca tttgagcctt cttacaacgt accaggtgca
     6421 gtacctcagt ggcaggcggc tgttcttgat cgagcaaaaa cgaatttcga aacattaaaa
     6481 aaccatccaa gtattctttt ttggtctctt ggaaatgaat cttatgcgga agaaaacatt
     6541 cgacaaatga acgattactt caaaaaagta gaccccgatc gacttgtcca ttatgaaggg
     6601 aattttccaa atccagcatt cgaagattcg atttcagatg taaaaagctg catgtatgcg
     6661 ccaccagaag agatcattga ctaccttgaa aataaagcgg aaaaaccgtt catcttatgt
     6721 gaatatatgc atgacatggg gaattcgtta ggtggaatgg attcttacat gacgttgctt
     6781 gatcgttatg aaatgtacca aggaggctat atatgggatt ttaaagatca ggcgatccaa
     6841 gtgactgatc caatcacagg cagagaagtg atgcgctatg gcggtgactt tgacgataaa
     6901 ccaagtgact atgaattctc agggaatggg atcgtttttg cagatggaca agaaaaaccc
     6961 gccatgcagg aggtaagata ttattatgaa aaatacagta aataaaagtc atatggatac
     7021 ggaaaaagtt gcaatcgtct tcggcgactg tacattaggt gtcaaatcgg ggaatacgca
     7081 ttatattttt tcttatacaa gaggcggact ggaatcgctc aataaaaacg gaaaagaatg
     7141 gctataccgg gaaacacttc cagttttttg gcgagcaacg accgataatg acagaggtag
     7201 cggatttgga tttaaatccg gtatgtggtt aggagccgga ttgtatccaa aagtcaaaga
     7261 aatagaagta ctggtggaca ataaaaagat cgatttgccg attgctccag taaataatca
     7321 atattcaagt aatgaatacg ctgaaacggt tgaaatcatt tttacactgg aaatcaacac
     7381 agtaccaaaa acccaagtga caatttccta ccaaatcgct ggagagggta aaatgcttgt
     7441 caatatgcat tattatggac aaaaaggatt accggaatta cccgcattgg gtttaaggtt
     7501 cattatgcca acgaaagcaa aattttttga gtatgaaggc ctatcagggg aaacatatcc
     7561 ggatcggatg gctggtggtg tcgaaggtat ttataaagtg gcaggactcc ccgtttcccc
     7621 ataccttgtt ccccaagaat gcggcatgca tatggaaacg aaatgggtag aactaacaag
     7681 aaatcaaacg ttggataata cggataaaga tcaagaagat ttttcgttgc gatttgaaca
     7741 gcaggatacg ccatttgctt tttccgcttt accattcaaa gcagaagaac tggaacaggc
     7801 aacacatata gaagaactac cgccagaaag acgaacggtt cttacgattt ttgcaaaagt
     7861 aagaggagtc gggggtatca atagctgggg agcggatgtt gagtcacctt accgaatcag
     7921 cagcgaagag gaccatgaaa tcggctttat tattcagtag caatctttag attattattt
     7981 gaaaaacgga gtttacaaga caaagctgct ttattctata tgtcatgaac aagagagtaa
     8041 aaaagttctc agatactttt gttcatgact attttcttca ataaaaccaa ttgttatttt
     8101 tctgaaaaac aaagttaact atcagaaaa
//
LOCUS       DAZULO010000107         8085 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000107 DAZULO010000000
VERSION     DAZULO010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.109"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            169..870
                     /locus_tag="ACG8OA_002466"
     CDS             169..870
                     /locus_tag="ACG8OA_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4977209.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1479..2495)
                     /gene="tsaD"
                     /locus_tag="ACG8OA_002467"
     CDS             complement(1479..2495)
                     /gene="tsaD"
                     /locus_tag="ACG8OA_002467"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFS4977210.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2492..2956)
                     /gene="rimI"
                     /locus_tag="ACG8OA_002468"
     CDS             complement(2492..2956)
                     /gene="rimI"
                     /locus_tag="ACG8OA_002468"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS4977211.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2963..3505)
                     /gene="rimI"
                     /locus_tag="ACG8OA_002469"
     CDS             complement(2963..3505)
                     /gene="rimI"
                     /locus_tag="ACG8OA_002469"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS4977212.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3489..4211)
                     /gene="tsaB"
                     /locus_tag="ACG8OA_002470"
     CDS             complement(3489..4211)
                     /gene="tsaB"
                     /locus_tag="ACG8OA_002470"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFS4977213.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4402..5382)
                     /locus_tag="ACG8OA_002471"
     CDS             complement(4402..5382)
                     /locus_tag="ACG8OA_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4977214.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5406..6890)
                     /gene="galT"
                     /locus_tag="ACG8OA_002472"
     CDS             complement(5406..6890)
                     /gene="galT"
                     /locus_tag="ACG8OA_002472"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFS4977215.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6902..7891)
                     /gene="galE"
                     /locus_tag="ACG8OA_002473"
     CDS             complement(6902..7891)
                     /gene="galE"
                     /locus_tag="ACG8OA_002473"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFS4977216.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2293 a         1721 c         1417 g         2654 t
ORIGIN      
        1 attcgcttca aaacaaatta ttcaatacca ttgtatattt tttacataat aacaaattat
       61 atttgtaaag tattaaaact tgtttctaat gttggtatgt taaaacttta caaattcttt
      121 aaaaaaagtg acaataaata tagtgaacaa taaggagagg actttttaat gcgtgttaat
      181 aaaatcgatt ttcacttttc aaaactcaga tatcaacctg attttgaaca tttctcggat
      241 gaagagttcc aacagatcaa ggaacatact gttcttcgtt cttataaaaa aggacagatc
      301 cttttcgacg atggtgatcc cagacaacgg ttgtatatct tgaccaaagg actcgttcgt
      361 ttagaacgtt atgatgaaag tgcaacttat tactactatg actatgtgaa tacaaataac
      421 ctttttccta tgtcaggaat gtttggagaa gagacctaca cttatacagc tcaagcgatg
      481 actgatattg aaacattcta ccttcctgtt tcctactatg aaaaattagc acgaaaaaat
      541 cccgaacagt tgatctatat gatacaaaaa atttctaaag tagttgaaat acatgaattg
      601 cgtatccaaa tcggattgac ctctagcgcc tttgaccggg taaaacaaaa tttattcatt
      661 ttgaaagaag aattgggcgt accaacaaaa gatggtcata tcaccattcc ttatccgatc
      721 accttaaaag aattagcagt gaatagcggc acgacacgtg aaactgctgg acaagtaatc
      781 aaacaactac gtgattccgg gctgttagat tatgacaaaa agcgttttac tttctgtaag
      841 acagctacta ctccagctat gagagaataa cgcgaacaaa aataagcagt gaccctgata
      901 aatatcaggg tcactgctta ttttcctccc taaaacttct gaaagatccg gcagttttag
      961 atcactggac aagactcaaa gattttctat gtgtgtttca gttatgtaat attcactgtt
     1021 tgcaaaaaat actgttagat taactagatg aagcgtcttg tcctcatccg atctagatta
     1081 ttcgatattc ctcgacaata aacgattact cagtgagggc aatcagagga acggataatg
     1141 ataaataagc tgtaaaaaat agcaaaataa caataatgat taaaagtatg taaaaaatag
     1201 ggctttgaca aaaacgtgtc acagccctat ttcgattaaa cggtgtgtac ctgtcaactc
     1261 ttcggtcaca agtcataatg ttcataaaca tgaggagctg ttttttgaaa ttgttccttg
     1321 tgaatcagag ttaaacgaca ggcttacgaa ctcttgatta aacggcgttc aaaagcttgc
     1381 atacgcggta ataagaacag ccaggtcaaa aacatgagaa gccgtttttc ctgggctgtt
     1441 cttattactt gatgcagggc agctttttca caacctcttt attcagataa aaccaagctt
     1501 ggatcagcat tcaactgata gctagcaaaa tttctttttt gcatttccac atgtgctgct
     1561 gctccaatca ttgcagcatt atctccacaa agtcgaagag ggggaatcac tagttctacc
     1621 tcagggagtt tagctgccaa agcatcctgc aatccttcac gcaacccttg attggccgct
     1681 acacctccag ctataatcag ttgcttgact ggataatttt gacaagctcg aagtgtttta
     1741 gtaatcaata catcgatcac acttgcttga aagctagctg ctaaatcatt tttgtctaga
     1801 tcttctccac gctgttgtgc attatgcacc aaattgataa atgcactttt taatccgcta
     1861 aaactgaaat cataattatc ttcatggatc atcgctcgcg gaaaatggta attatctttt
     1921 ccctgatgag ctagctgatc gatttctttt ccgcttgggt aagaaagacc taatactctt
     1981 ccaactttgt cataagcttc tcctgctgcg tcatctcttg tctccccaat gatttcatat
     2041 gatccatctt cttgcatata gactaattct gtatgtcctc cgctaacaag aagtgccatc
     2101 aatggaaact ggaatggttt aaccagacgg gctgcataga tatgccctgc catatggttt
     2161 acaggaataa gcggcaattg gtgtgcccaa gcaaaagcct tagctgcaga aataccaatc
     2221 agcaacgagc cgacaagtcc tggtccataa gtgacagcga ctgccgataa atcttctgcc
     2281 gacacaccag cttctaccag tgcatcttcc aagcataagg tgatttgttc tacgtgatgg
     2341 cggctagcta cttcaggtac tacgccacca aaacgtttat gactgtttat ttgagaagca
     2401 acgatgttag ataagatctc tgttccattt ctaacgaccg ctacactcgt ttcatcacaa
     2461 cttgattcaa tcgccagaat caattcttca ttcatttcat cgtctccgtt tttaactttc
     2521 tgctcataat gatcgcatct tccgtcggat tgcgataata tgatttccgt ttacctaata
     2581 tacgaaactt ttctgattca taaagttttc ttgctgcttc attagataga cgtacttcta
     2641 aaaaaacatg cagcgaatct tttatttgtt ccgtaatcag taattgtaac aaatgccgtg
     2701 catgcccttt tccttgttct gaaactgcaa cagcaatatt tgtgatctcg acttcatcta
     2761 ttactttcga gtatccaata aagccacaga tctggttatc ttcagctaaa agcagataat
     2821 cagagtgagg ctgttggatg tctgccaaaa actgcatttt cgtccaaggt gaaccatgtg
     2881 gataagcatt ttcgctaatc atccatacgc attctgccaa gtacgcttca tcaaaatctt
     2941 tttttctgca aatcaactaa tctcactcat tttctaaaag ttttatcata tccaacgcat
     3001 cttcttttgt ttcagtgtaa tatctttttt tgatcgttgt tgcttgaaac cccaatttcc
     3061 gataaagtct ttgtgcatct tgattactca tacgcacttc taatgacatc gtctcacagc
     3121 gattcattat agcaaagttc tcgatttcgt caataagcag cgaaccgatt cgtttacgct
     3181 gatatgccgg tcgaacggca atattcgtga tatggcaatc taaaccgaac gctcttgaac
     3241 cgataaaacc aatcattctt ccattatcaa agatacctag atatagatga ggaagcggtg
     3301 acttcaattc tgatataaag gcactttttg tccatggcaa ttcgccatta tagacttctc
     3361 gttcgacatc cagcagctct ttgatatctt ctgtagtcaa ttctcttagt acgtaacgac
     3421 gatcgatttg ttcgactgtt ttttctgtat aaatcatttt atcttgaaaa aagcttctgg
     3481 catacgctct aaattttttc aacataactt tcgtctccag gtgtgtgcgt ctccaaccat
     3541 ttttcttctg cttctactcg ttttaaatag cgaggaagaa aagcttgttt ctctgcaact
     3601 ggttcaacta aagcacctaa ttgagccaca gttacaccac ttggaatatc ccatgctgga
     3661 actgtattaa tcatcgcact aggcaatatt tcctttattt cagagaagaa tttgtgacaa
     3721 tcactcccga cgaaataaat agcttcattc agcttttgta gttgtgtgaa caaatcagaa
     3781 gcagaaatat gtacatcttc aagtacagtt tctaatctat tttctttcca gcggtatgcc
     3841 ccagcgtata cattttttct tcgagcatca aacatgggca caataatatt atccacaccg
     3901 atgcaattag ccgcgatggt tttcagacta gatactccta ctagctcttt atctaatgta
     3961 taagcaatcg tttttgctgt cgttacacct atacgaagcc ctgtataaga accgggacca
     4021 tcagaaaccg cgattcggtc aatgtctttt ggacttattt gaagatctgc aaacaattgg
     4081 tcgattgcag gcatcagtgt tacactgtgg ttgcgtttga tatttgtttg tatttgtcca
     4141 agaatttgtt cgtcttctac cacaccgatc gccaaagttt ggtttgcagt atctattcct
     4201 aatgtaatca tatatttgat cctttctgct atgacattct cgccttccat tgtagcatac
     4261 ttttttattc tgaggatgaa aataccaaag gcaaaactag tacaataata catgattatc
     4321 tgctggcggg caaattttgc cccttcctga aacacaaaaa agctgcggta tcactccatt
     4381 ttcccacagc ttttccatct tttatttcgt tgattctcga ataatcaact ctgtttccac
     4441 aataatcttt cttgaaaccg gtgctttgga attacttaat tgaagaatcg tctccactgc
     4501 tagctctccc atccattcgg tataaacttt tacagtagaa agcgagggcg ttacatattt
     4561 cgctacactg acatcattga agcccatcac agaaatatct tctggtacca ctaaaccttt
     4621 ttcttgaatc gctttcagtg cgccaatcgc taatgcatca ttagctgcaa aaagcgcatc
     4681 aggaacatgc ggattcgttt ttaaatattg cttcactgct tgatatcctg actcgacaga
     4741 aaaatctgct tcgataattg gaccttcgtc gtagcctttt tctgccaaaa cttgttgaaa
     4801 tgccataatt ctgggatctt ttagccgctg atggtttttc ttcgtatatt ctgttccagc
     4861 aagcatagcg atatgttgcg ctttatggga gacaaagtgt tcaatgactt tttgaacact
     4921 tgacttaaag tcgacaacta gagagtcgat tccttgagaa gtgccgtcag aatctaccaa
     4981 aagcaaggca ttttgggatc ctttcatttc taataactga gcttcgtcga attttcctag
     5041 tgcgatcgtt ccatctgccg gaagatcact aatggaatcc cacgtttctt tcaataaagt
     5101 aacgtttgct tcttctgcct ttttttcgat tcctaggcga atagataaat aataaagatc
     5161 ttcaagctct tccgtatcgt cgtaccactg gatcaggcga agcgtttgct tagtatttgc
     5221 tgcattttta cgatgtttcg tataatttaa gcgttctgct gcttcaaaaa ttttttgctt
     5281 tgtggctaag ccaacggaaa gatcaggatc atagttgaga actcttgaaa cagtagcagg
     5341 agaaactcct gccaactgcg caatgtcttt aattgttgcc atttgtctct cctccttttt
     5401 tacgtttata aaatcgcagt aaatcgttca aacgctgcct gtcctttttc atctcgcttg
     5461 aatactccag cgtcttccag tacacgtgca aaaacctgac ctacagattt ttgtacgaca
     5521 tcggtgaccg tatctttcgt caaatcagga tgttcttttt ttaattgttc tgcccacgga
     5581 cgatgatatt cagccatctg gttttcttct cctaataaga aatgctctac ttctactagt
     5641 tctggtttca aacgaggtgg taaaacagca agccccatta cttcgatcaa cccaatgttc
     5701 tcctttttga tatgttggac atctttatgt ggatgaaaaa tcccatcagg atgttcttct
     5761 gaaacattgt tgtctcgaag gacaaggtcc aactcaaagt aattgtccct tcttcgtgca
     5821 attggcgtaa tcgtatggtg tggtgtacca tctgctgaaa aagctctgat agatactgtt
     5881 tcatccgaat atgctttcca tttttccaat actgagttag ctgcttcaat aagcaagttt
     5941 ttctctgttg attgcaaacg gatcacagac atcggccatt tcacgactcc tgcgatgact
     6001 tgcggataat cttctagttt gataagtttt tctatcggtg cttttgccat aggaaattca
     6061 tgacgtcctg cctgataatg atcatgagaa agaatcgatc ctccaacaat tggtaagtca
     6121 gcattcgaac caacaaagta atgaggcaag acttcagtaa tccgaagcag tctagcaaat
     6181 gtttcctttg tgattttcat cgggcggtgt tgttctgata aaatgatcga atgttcattg
     6241 taataagcat aaggggaata ttggaatccc cagctttcac cgtctagatt cattcgaata
     6301 atccggtgat tggtgcgggc tggatagttc aagcgacctc tatatccttc gttttccatg
     6361 caaagcatgc aggtaggata gtctactttt accgcctcgc gttctgctgc gatttgtttt
     6421 ggatcttttt ctggcttaga taaattgatt gtgatttcca gttcaccata ctccgactct
     6481 gccggaaatt tgatgttttt tgcgatggct cttgttttga tgtagtcatt tttcttactt
     6541 aaattaaaga aatactcagt tgcttcctct gggcttttcg aatagtgctg tgcaaagaat
     6601 gcattgacga ctgaaggtgg cggtgtgaga aaatccatta attgcgcttc aaaaatctct
     6661 tgttctgctg gcaattctga aagaacacca ttttctcttg ccttagcgac aagctgatcc
     6721 aataagtcta cagattcttc cttaactggt cttacttcaa tgctttctag agcatcttct
     6781 cctaccatcc ctaaaagacg attctgtaaa tataagcggt ccatttccat ccatccgcca
     6841 gcttcgatcg caagtgtggc aaaatcacaa atcgtttgac tgattgacat atctcattcc
     6901 cctattcttc ataaccattt ggatggctga catgccaatc ccatgctgtt ttgataatcg
     6961 ctttgatatc tgtgtattca ggtttccagc ccagaatctc tctcgctttt tcacttgaag
     7021 cgactaatcg gctaggatct ccagctcttc ttggtgctat ttctgctgga atttcttttc
     7081 ctgtcacttc tctagctgct tctagcattt ccttgactga gtatcctttg ctgctgccta
     7141 aattgaagaa gttgctttca tttccttctt tcaagtattc caaagctaaa atatgtgcag
     7201 caatcaaatc ttctacttga acataatctc tgatacatgt tccatctggt gtatcataat
     7261 cgtctccata aacagccaag gcttttcttt gaccaagagc tacttgtaaa atgattggga
     7321 caagatgcgt ttctggtgta tgatcttctc cgatagatgc atctgctttt gctccggcta
     7381 cattgaagta gcgcaaagcg acatagcgca tgccatatgc ttggtcacac cacttcatca
     7441 ttttttccat catcaattta ctttcaccat acggattttt aggattagct ggtgtatctt
     7501 ctgtgattgg agactctttc ggctctccat aagttgctgc agtagaagag aatacgattt
     7561 tattcacgtt gaactcgtgc atgacttcaa gtaagatttg cataccgtat acgttgttgt
     7621 tgaaatacat caatggtttt tcgacagatt ctcctactaa agagcttgca gcaaaatgga
     7681 ttactccttc aatcggttcc ttttcaaata cggaacgtag aaattcttta tcccggatgt
     7741 ctccttcata aaaatgagca tctggatgta ctgcttgttt gtgacctgtg agcaaattat
     7801 ctaccacagc tacttgatac cccttttgaa ctagctggtc tacagcgtgc gaaccgatat
     7861 aaccggctcc gcctagtact aaaattgtca taaaaaacac tcctttgatt agtaaaattg
     7921 tgtttgttga ttctatataa tttaaaaaac gaagtttact tttatggtga aaacaaacaa
     7981 agtaaaattt cgtaaattca tatggtaaat ttataataaa acatttagta aacattttca
     8041 agggtttttt gcaaaggttt acaaacaaac gtaaaactag ccttt
//
LOCUS       DAZULO010000108         7907 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000108 DAZULO010000000
VERSION     DAZULO010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.111"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..995)
                     /locus_tag="ACG8OA_002474"
     CDS             complement(21..995)
                     /locus_tag="ACG8OA_002474"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS4977217.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="ACG8OA_002475"
     CDS             complement(1075..1950)
                     /locus_tag="ACG8OA_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFS4977218.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="ACG8OA_002476"
     CDS             2080..3987
                     /locus_tag="ACG8OA_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HFS4977219.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKKLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="ACG8OA_002477"
     CDS             complement(4528..7806)
                     /locus_tag="ACG8OA_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFS4977220.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         2238 a         1664 c         1465 g         2540 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaacttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatta gaaagcgttt acttaaagta ttacctaatt
     7861 tatttttact gtcaataaaa aacaaaatta taataagtgt attttat
//
LOCUS       DAZULO010000109         7799 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000109 DAZULO010000000
VERSION     DAZULO010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.112"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..355
                     /locus_tag="ACG8OA_002478"
     CDS             <1..355
                     /locus_tag="ACG8OA_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977221.1"
                     /translation="LAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMS
                     LKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGY
                     FPNKNNNFYFYRNKL"
     gene            complement(481..1158)
                     /gene="nth"
                     /locus_tag="ACG8OA_002479"
     CDS             complement(481..1158)
                     /gene="nth"
                     /locus_tag="ACG8OA_002479"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFS4977222.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1165..1863)
                     /locus_tag="ACG8OA_002480"
     CDS             complement(1165..1863)
                     /locus_tag="ACG8OA_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS4977223.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2131..3315
                     /locus_tag="ACG8OA_002481"
     CDS             2131..3315
                     /locus_tag="ACG8OA_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFS4977224.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3321..3764)
                     /locus_tag="ACG8OA_002482"
     CDS             complement(3321..3764)
                     /locus_tag="ACG8OA_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS4977225.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3990..4277)
                     /locus_tag="ACG8OA_002483"
     CDS             complement(3990..4277)
                     /locus_tag="ACG8OA_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS4977226.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4448..5800)
                     /locus_tag="ACG8OA_002484"
     CDS             complement(4448..5800)
                     /locus_tag="ACG8OA_002484"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFS4977227.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6073..6519)
                     /locus_tag="ACG8OA_002485"
     CDS             complement(6073..6519)
                     /locus_tag="ACG8OA_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS4977228.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6755..7681)
                     /gene="cysK"
                     /locus_tag="ACG8OA_002486"
     CDS             complement(6755..7681)
                     /gene="cysK"
                     /locus_tag="ACG8OA_002486"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFS4977229.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2108 a         1586 c         1271 g         2834 t
ORIGIN      
        1 ccttgcattt attatttttt ctgtactttt aagcactact cttaaaggaa aatttatgga
       61 aagaagctct catgcaatga ttctcacttt tttatggttg ttgttcacca gaatctatat
      121 ttttctgatg agcctgaaac cgcccaaatc cattagttta tgtttagtta taaatggtat
      181 ttttttatta tcacttatca tttttattac acctttagat agatacgtca cttatttata
      241 taatcctttg gaatactgga cttattttat aggtatattg gaaggcatta ttttctacat
      301 cgggtatttt cccaataaaa ataacaactt ttatttttat cgcaataaat tgtaataagt
      361 taccatccta gctactgagt atgtacattt actttttcat gcaaaaaaga taaacgaaac
      421 agcttttctc cataataaca agataattca tacatttatt tgcaattaaa gaaaccacgt
      481 ctacaggcgt ggtttctttt ttagcgcctt ttcttttaga cgcatccgat ttttcccatc
      541 ttgacatatt gatagcaaag gacatacctc gcacttggga tttcttgccg tgcaatgata
      601 tctaccaaaa aaaatcagtg tatgatgtgt ctttacccat aattcttgtg gtactttccg
      661 catcagtgtt tcttctactt ccatgaccgt agcatctagt ttgcagatcc tcaggcgttt
      721 tgagacacgt tccacgtggg tatctactgc gattgctggt atcccgaatg catcccccaa
      781 tacgacattt gctgtctttc tgcctacacc aggaagagac atcaattctt ctcttgacgt
      841 tggcacttgc ccatcgaaac gttcaatcag ctgctgtgca cacgctttga tgttttttgc
      901 tttatttcga tacaaaccaa tcgtttttat tttaagaatg atttcatcga ttgaagcttc
      961 tgctaacgca tcgggtgtcg gaaaagaggc aaacaaatct ggcgttgctt tattcactga
     1021 tacatctgtc gcctgggcac ttaagatcac tgcaatcaat aactcaaatg gattattgtg
     1081 cttgagttct ccatgtgctt cgggaaacat tccatacatc gtttctagag cttccattgt
     1141 tttttgtttg cttaacatat tccgctactc ctccaaccaa tttttcagtg agactttcgg
     1201 caattcttct tgttgagctg actgttctac ttctctttga agaagctgct gttttcttcg
     1261 tttttggtct tcttccactt gttgttttgt acgtagattt tttctttccc acgatagcaa
     1321 aatgcggtca acgtaattaa aactataagc ctgatttaat acggcttccc gcagtgctag
     1381 cttcaaaatt tccggctgat aatggtcttc ttctagccat tgtccgatcc gctggaactc
     1441 gatcgaagaa agcgggcgtc caaattcttg ttcaaataat tgatacactg actggatttc
     1501 tttttcttgt gttttttctt cgtatttttt ttcttgtgtt tttaaatact cacctaatag
     1561 atcatagatg gggtaaagat tatatcgatc tgctttttta ccgttgacaa tcgtcgtttc
     1621 tatcttgata aatccatttg ttacgagacg atccagtatt tgaaaaatct gatcttgttt
     1681 gattcccata tccatagcaa tcatctgcaa atcagggaaa gtatctcctt caagctgtgc
     1741 catatgcaac tgtagaacaa ataaaaattc ttcagattgt agaccgatcc tatgataatt
     1801 tttaagtaaa agattagata tgactgtttg tccagcgctt aagtaatttt ctaaattcaa
     1861 catgatttct cctccgctca atcacagtat acgtaaaaaa aacatgggaa gcaagaacag
     1921 gtttcttttc tatttacttc actgatcggt cagatttaca atgtcgtcta cttgattttt
     1981 gtttgttcat gtttttgatg cgtatcttca cttttcggaa taaataaatt aagtagattt
     2041 cgctgttcct tagaatgttg acaaggaaac aaaattacta tacactattt ttgtttccaa
     2101 gttaacatta ttgcataaag gagtttttac atgtacagtt tcacaaataa acattctaaa
     2161 gtttttggat acgtctctgt cttattcact ggattaggat ttactatcgt cagtcctgta
     2221 cttccgtttt taacgctgcc ttattctcac tccatacatc agcaagcttt ctatatcact
     2281 ttgctcatgt ctgtttatgc tctagctgcc ttcttatctg cccccattct cggtagctta
     2341 agtgatcatt ttgggcgtca tccgatcctt attatcagtt tattaggatc aagtatcgga
     2401 tatttgcttt ttggtttagg aaattcgata tggatgttgt tccttggtcg tatcattgaa
     2461 ggattgacgg caggagaaat ctccacctta tatgcttact ttgccgacat cacggaacct
     2521 aatgaaagaa ccaaagtttt tggatggatg ggtgctttag tcggtatagg aacaacgctg
     2581 ggtcctatta tagggggtct attagctgag ttggggaatt ctgttcctat atttattgga
     2641 gcactcttta ctttcttaaa tgccgtttat ggctacacat ttatgctaga aagccttcca
     2701 ataaaaaaac gttcagtaga tttaagtttc tcccatgtaa gaccttttca ccaattacaa
     2761 caattattca agatttcatc cgttattcct ttattgacag caggttttgc tgtttggttg
     2821 gcagctggct ctcttcaatc tatcttttct caattttcga ttgacgcctt tcagtggaag
     2881 gctgggttgg ttggtttaag cttctctttg attggaatct tggatactgt ctcgcaattg
     2941 ttcattatgc ctcgattgct caaaaaattt tcagaacagc agatcacaag aatagggatg
     3001 ttcagtgaaa tcctcgccta tctattcatc acattatctg gaattcttct actgccgatt
     3061 ttgtttttat ttgggattat ctgttatggt ttcggtgatt ctatttttac tcctgtattc
     3121 aatggccaac tatccaattc tgtctcagat aaccagcaag gtcttgtaat gggcggtacc
     3181 caaagtatac aatctctatc gcgcgtcatc ggcccgctaa tcgcaggaca gctttatgct
     3241 gcagcaccat ttcttccgac ggtattcgga tttttgctta tgatttttgc ctattatcac
     3301 tacaataaaa aatagtagct tcatttgcta cttcgtataa attctctgta tcgaccgata
     3361 aaacgaaata ctgtgtcaaa ttcttctttt gacatatttt caaatacagt agcatctcgc
     3421 tgtacccatt tttgatggag ttgctcgtgt ctattcctta ttttttgtcc gattggcgtc
     3481 aattgaaaat agatttcttt tttattctct tcgctttgat aagtgtttat gatccctcta
     3541 gctatcagtt tttttgttag cttactgatg gctcctctag tcatgtaact agcattcgct
     3601 aattttgtca cgttaggatc agggattttc ccgatatgtt caatcacttc tagttcattt
     3661 agagtaaatc ctgcaaattc tttttccatt tctggtttat tacgatgaat aatctcatta
     3721 gctaaatcca gatattcttc tattagcagt tgtcttttat ccatttctac accttctttt
     3781 tctttcatta tattgttttt ttgtttccta ttcaacaaag ataaaaatat tttgtaggaa
     3841 aacccaatct gaaaccaaga aaaaaagggg ttgacaaagc ttgtcataac ccctttttct
     3901 gattaaaaga tagtaaagag acagtcatcg gtaattgtga tgattttttg tcatcactta
     3961 aggatgagcg gccttttcaa aaactgtctt taaagatcat catcagtttc tagcacatat
     4021 ggctgacctt catatgtgta ctctgtattc aatgtgccgt cattttcatc tgagtagatg
     4081 cgtctactga aaggaaaacg atgagaaaca ttatctttgg cgtcatttgc tggcatcgtg
     4141 taatattcct cttgcgtttc attcaactga gagagtactt gatctgccaa ctcatcaata
     4201 ttgatcgtta atttcttcga agcactttta taatcttcta aaaatgcatc ttgttggaca
     4261 aaatattctt tattcatctt ctttctcacc tttcttttca aaatcaaata aagctgcttg
     4321 ttcttttatg taacagcttt atctttgctg cttattgtga caaaaaattc gaagaaagac
     4381 aactaattgt acttttcaca aaaaaagaca gagggtcgtg acaaaattgt gtcacaaccc
     4441 tttttcttta ttgtgcagtt tcgatttcgc ctagcgctgc ttgtgcaagt aggttcaagt
     4501 aattccatgg gcggtcaaaa tgtggttgga agaagaaatc tgaaagtgcg agatcttcta
     4561 cagtcatttt attttgaaca gctaaagaca tggtatttgc tgattgtgta atgtcgtatt
     4621 tggacatgaa ctgagcacct acgatacgat tcgttccttt ttcatagact agctccatca
     4681 ttactttttc agtagttggc atgaactctg gacgataatt atcttcaaat acagtcgctt
     4741 ggacttctag atcgttcaaa gttgcgcttt ctttggtcac acctgtcgaa ccgatcgtcc
     4801 agccaaatag gtaaagtcct gaagttcctt gagtaccacg atacgccatt ttatgatctg
     4861 tcaagttatt tcccacaagc aaaccttgac gaacggcatt tgttgccaaa ggaatatagt
     4921 tttgcgtgcc acttggattg tagttgacta ctgcagagtc tccagctgca aaaatgtctg
     4981 gattacttgt ctgcatgtat tcattcactt tgattgcccc gtttggcagt gtatctactt
     5041 ttccttcgat cagttttgta tttggacgga agcccacaca taaaatgacc atatctgtat
     5101 caaatgtatc tgttggtgta gctacttgtt tcacttttcc ttcttcatct gtgataaatt
     5161 cagtaacatt ttcgcctagc gcaagagtaa caccgcggtc tgtcaattct ttttctaaga
     5221 tatctgtgaa tggtttatct agatatttat tcaagatgcg atctaatcca tcaatcaatg
     5281 tcacttgttt tccagattcg acaaaagctt ctactaattc aataccgata tatccaccac
     5341 caacaattac tacacgtttt gcttctttcg cttgtgcaat aatttcattt gcttgattgt
     5401 agtttttgca taggagtaca tttttcgcat cgattccact aatcggtggg ataataggcc
     5461 atgaaccagt tgtcatcact aatttatcat aagaaaccgt ttctgtttct cctgttttca
     5521 gatcttttgc cgttactgtt ttattttctg tatcgacgtc tgttacatcg tgttccattt
     5581 taactgttgc tcctaatgaa gataattctt caggatttga gtagaacaag cctgctgggt
     5641 cctttactac tccgccgaca taaagtgcga taccgcaaga taagaaagaa atattgtcat
     5701 ttctttcata tactgtcact tctgcttctg gatgattttt taaaatactt tttacagctg
     5761 cagtacctgc atgagtacag ccaattacta ctactttcat ttatggtccc tccgattaac
     5821 attttgtcct tcgacaaaac tactataaca gaaataaaac ggatacaaca gtttaatgct
     5881 tgtgaattca caaaggtttg tttgtagttt cattcctttt caagcataag aacccaatat
     5941 ctacaagatt cttattttaa taatattcac ttttatatct aagattgtga ataaaagaca
     6001 aaaaaataaa aaagctagcg ttttttttac acagctagct ttttttaggt ataacacttg
     6061 tagaaactgt ttttacattt gcgccttttt tgctgactct ttacaatccg gacagagtcc
     6121 atacaattcc aaccgatgtt cattaatctc aaaatcagtc agtctgcttg cagccatttc
     6181 tatatcttca agccctgggt aatgaaaatc aacaatctta ccgcagtttt cacaaattac
     6241 atgataatga cgctttgaac tgaaatcaaa tcggctagaa gcatcgccgt acttcatttc
     6301 ttgaacaaat ccaatcgaag taaataagcg aaggttattg taaacagtcg caacactcat
     6361 atttggaaac cgatgttcaa gtgctttata aatctcatca gccgttggat gggaatgatg
     6421 ttcaatcaaa tattctaaaa ccgcgtaccg ttgaggtgtg attcgaatgt tctctttttt
     6481 taacttttcg ataacctctt cgactaactt attttccatg taaacacccc acgttgttat
     6541 tctcgtctta tcatactatt tttacatttt aaaatccact taacatgaaa agaaaattca
     6601 attttattgt attttaatga aaaaagacgt aaaacagtcg cgttcactat tctacgtctc
     6661 tattcaaata atttttctca tcataaattt ttgtgtgttc ttttcatctt taaaagtttt
     6721 ttacataaac ctatctggag aaaaaccaag tgccttatat ctcttggtaa agggctgtag
     6781 ataaataacg ttcaccattg tcgggaacca cagctaatac cttcttgcct tttcctaatt
     6841 cttttgcgac ttttagcgcg gcagcaattg cagcgccaga tgagatgcct actaaaatcc
     6901 cttcttttcg cccaacttct ctagctgttt ccatcgcttc gtctccacta atagaaagga
     6961 ctttatcata cacagaagta tccagtgttt taggaacaaa acctgtaccg attccttgga
     7021 ttttgtgagg tcccgcttct ttcccttcta aaatagcaga ttctgctggt tctaccccga
     7081 ttaattcgat tttcggatac acacgtttta attctcggcc agcgccagtg atcgttcctc
     7141 ctgtaccgat acctgcaaca aacgcgtcca agccatttac tccaaatgct tgatgtatct
     7201 caggaccagt cgtcttctca tgcacaagag gatttgcttc attttcaaat tgtaatggta
     7261 aaaagtagcc gttttctttt gccaaacgtt ctgcttctct gattgatcca gaaataccat
     7321 ccgcaccagg agtcaaaatc aactctgcgc cgtacccttt catcaataaa cgtctttcga
     7381 tactcatcgt ctcaggcatc acgatgatta ctttatatcc ttttgcaact cctaccatcg
     7441 acaatccgat tcctgtattt ccagaagtag gttcaatgat agtatcacct ggtttcagta
     7501 gaccatcgtg ttctgctttt tctatcatac ttaaagcaat acgatctttt acacttcctc
     7561 ctggattaaa aaactcaagc tttacaaaaa catctgctga atcttctggt acgattttat
     7621 ttaatttgac gattggcgtt ttgccaatca attcggtgac tgagttatag atagctgtca
     7681 tcttcaattc ctccttgtct ttgctcttct tacttatcat aatacaatct gttcatttat
     7741 ggcgagtgat tcgtctttga ctagcaaaaa aaagacaaag atataccata tatggtaat
//
LOCUS       DAZULO010000110         7708 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000110 DAZULO010000000
VERSION     DAZULO010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.113"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(97..1245)
                     /gene="nagA"
                     /locus_tag="ACG8OA_002487"
     CDS             complement(97..1245)
                     /gene="nagA"
                     /locus_tag="ACG8OA_002487"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFS4977230.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1321..2157)
                     /locus_tag="ACG8OA_002488"
     CDS             complement(1321..2157)
                     /locus_tag="ACG8OA_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4977231.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2341..3558)
                     /locus_tag="ACG8OA_002489"
     CDS             complement(2341..3558)
                     /locus_tag="ACG8OA_002489"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS4977232.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3707..3868)
                     /locus_tag="ACG8OA_002490"
     CDS             complement(3707..3868)
                     /locus_tag="ACG8OA_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977233.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3941..4024
                     /locus_tag="ACG8OA_002491"
     tRNA            3941..4024
                     /locus_tag="ACG8OA_002491"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3975..3977,aa:Leu,seq:gag)
     gene            complement(4264..5634)
                     /locus_tag="ACG8OA_002492"
     CDS             complement(4264..5634)
                     /locus_tag="ACG8OA_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFS4977234.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            5727..6076
                     /locus_tag="ACG8OA_002493"
                     /pseudo
     CDS             5727..6076
                     /locus_tag="ACG8OA_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(6348..6638)
                     /locus_tag="ACG8OA_002494"
     CDS             complement(6348..6638)
                     /locus_tag="ACG8OA_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977235.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(6846..7538)
                     /locus_tag="ACG8OA_002495"
     CDS             complement(6846..7538)
                     /locus_tag="ACG8OA_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS4977236.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         2348 a         1528 c         1182 g         2650 t
ORIGIN      
        1 acttttgaaa cttatgtaaa gtgattttta ctagaacaca aaaaaacctt aaaaccaaac
       61 ttaagaagtt tagttttaag gttaacttaa attatattat gcgtggaaac gttccacgcc
      121 atctagataa gttgcttcca atgtcatatc agggttcaat acgataaagt ctgcatcgcg
      181 tccttgagcg atttttccac atgtatcatc gattttacag ctgattgctg gaatcagact
      241 agccatcatg actgcttgtt ctggtgttgc taatccccag tctacaacat ttttaattgc
      301 ttctttcaat ttcaatatac ttccagctaa gttgccagat tctagacgtg ctgttccttc
      361 ttttactact actggaaatt cgcctaaatt gtaatttcca tcaggcatcc caccggccat
      421 catacagtct gtaattaatg cgacatgatc atgtcctgct ttttccatta ggattcccgc
      481 agcatttgga tgtacatgat ggccatcaca aatcaattca gaaaagacat gttctaaaga
      541 taataacgca cccaccatac ctggttcgcg atgattcaat ccacgcatac cattgaaagc
      601 atggacaaat acacttgcgc ctgcttcaac agctttttgc gcttcttcta gtgtagcatt
      661 actgtgtcct agagaaacaa ctactccttc ttcagtcact tgtttgacaa actcttctac
      721 accttgacgt tccggtgcta aagcaatttt tttgattaaa ccacctgaag cttcttgcca
      781 ttcatggaaa gtatccaaat caggatcccc gaaataactt gggttttgtg cccctttgtg
      841 ttcttctgtg aagaaaggac cttcaaaata gattcctttg attttagctc ctggtgcttc
      901 ctgatacatt ttgccgattg tctcagctac atcttttaaa cgttctttgc ttgacgtcaa
      961 tgttgttggt aaaaatgaag tcactccaca agataacaga ccttcagaca tcactttgat
     1021 gccttctgca tcattatcca ttacatcatg attcatatag ccgtgaatat gtgtatctac
     1081 caaaccagga gcaatccatt tgccgctttg gtcaatgatg ttgacttctc cctcaggtag
     1141 ttctttggtg tattcaccaa aaagtccatc ggtaatttca agatagcctg catgtttgac
     1201 acctgacgct aaaaagaatt tgtcagcata gataaaagtt ctcattctat tctctccctt
     1261 tgtttttcta cctctaaagt actcttattt agagaagaag tcaagaatgg tctacaccat
     1321 ttacaaattt tttgaaattt gtttcacagc ttgtgctaag ccttcataga atgctgctct
     1381 ttctggttct tctttacgct tgtatattaa gcttttaagc acaaataaat cattgaggta
     1441 atagtagcta ttcttctctt gagcccaata gattccttga ttcacctgtt ctaacgcttc
     1501 gtcgatttca ttttgatgga ataagaaatt tccataatta taaaaaattt gcgaaaattc
     1561 actttttgat cgttcatcag ctgattgatg cagcagttgg atgcttcgac tcaaatagta
     1621 gcgtgcttca gccgtttttc ctgccacacc atatattttt ccgatacaac taatcagttg
     1681 tatttctgtg tcagaaacat ggattctttc tttttggcta gagtagctta atgcttcttt
     1741 caaataataa agggactgag agatattttg acgcaacgtg aattcgcaaa caccgagata
     1801 ataataatag cgttgaaagt cagttgcttg atacagattt tcagaaatat cagaagattt
     1861 tagtacttgt tctaacttcc gataatcacg tttttggaaa caactatcca acagttcaaa
     1921 taatttatcg tttgtccgag tagtttttcg agagagattc atcacatgat caatcgttac
     1981 ccctagccgt tgacaaatct gcatcatcac caatacattc ggcagttcct cattattttc
     2041 gatccgactt aatacacttt gtgaacaaat atcatgcgaa agcatttttt gcgtcatttt
     2101 ttgttctttt cgaatctctt taatgatcgt tccaaaggac tcgattaacg tttccataag
     2161 tatcctcccc aaaatatgcg tattcgcatt ctattcgtat tttactttag gaagtcgcaa
     2221 acttcaagaa aagacttgca actaaataat atactttagt tatttaagaa aactccacta
     2281 gaaagagagc ctcagtgtgt ttgtctgtgg ccctcctact gaaaaatttg gtttttaaaa
     2341 tcatttttgg cgataggttg ctaggaattc tctcattttc ttagctgttt gtttcaaatc
     2401 atccatttta ggaagataaa cgattcgaaa atgatctggc tggttccaat taaatccacc
     2461 accatgtaca agtagaatat gatgttcatg taaaaagtcc aaaacgaatt tttcatcatc
     2521 caaaatattg aatcgctttg tatcaatttt tgggaatata taaaaagcag cttttggttt
     2581 tacggctgaa agaccaggaa tatcattgat tgcattatag atatattctc tttgttcata
     2641 gatccttccg cctggcagga gcagctcatc gacactttga taaccgccta gcgcggtttg
     2701 gataatttgc tgagacaata cattggaaca caaacgcatg gaggacaaca tattcagccc
     2761 ttctatatag cctttgacgt gtgacttatc tccacttagg accatccagc ctactcgaaa
     2821 tcctgctaca cgatgagatt tggatagacc gttcagcgta acaacgaaca aatcaggcgc
     2881 taatgtagca atcggaatat gttctagacc atccatcact aagcgatcat agatttcgtc
     2941 ggaataaata atcaggttat tttgtcgagc tacttcaacg atttcttcta ataattcttt
     3001 tggataaagt gccccagtag gattattagg attaatgata acaatagctt ttgtccgact
     3061 cgttattttt gatttgatat catcaatatc aggataccat tctgcctctt catcgcaaat
     3121 ataatgaaca ggtgtgcctc cagctaatgc aatagaagcc gtccacaacg gatagtccgg
     3181 cattggaacc agtacttcat ctccattgtc cagcaacccc tgcatacaca tcgtgatcaa
     3241 ttcgctcact ccgttgccgg tgtagatatc attgatcgtc acattcggaa aatgtttcag
     3301 ttggcaatat tgctcaattg cttttctagc agaaaagatc cccttggaat ctgagtagcc
     3361 ttctgagtca cgggcattca tgatcaaatc acgaatgact tcgtttggtg cttcaaatcc
     3421 aaaaggtgcg ggatttcccg tgttcaattt taaaatacta atgccctctt cttgcatccg
     3481 ctccgcttct tctagaacgg gaccgcgaac atcgtaactg acaccttcta atttgttgga
     3541 tttttcaaat tctctcatca cttagccaat ccctcatttc aaaaagttag aactttccaa
     3601 attttttgtt atttactatt tcaatatcat actccatcct agaaaatttt gccacttatt
     3661 taggaaaaac gtaagtatta aaaaaacaca aacaagcgtt gagaacttaa ttgtcatgat
     3721 agtcattcca tagattcgct gcccaatcca ttgcaattcc acaaatgatt cctagcataa
     3781 aaccaagcaa aaaacgtccg aatgcaagtc caatcaatac acctaagatc attcccagtc
     3841 ctgaaaattc tccgtcatat ttctccatct taataagtcc tcccattttt gagcttgaca
     3901 actatgtaaa acctcgctat cattagtgtg ttattattac gcagatgtgt tggaattggc
     3961 agacaagcat gattgagggt catgtgagcg ttagctcgtg tgggttcgag tcccatcatc
     4021 tgcactacaa ctcgtgaagt taattttgct tcacgagttt ttttgtttga acgtttcttt
     4081 atgccattat atacatatca ctgtatcctt tacaaaaaga acgtacaatc atgtaaacag
     4141 cctgaggtga gtatatgaag gtcggaaaca agctactcgt agaaacttgc aagtgttaaa
     4201 acaattaatc gaaaaacgag taaaatccct cacctaatac taaagcgagg gattttttgt
     4261 atattatctg tttatcaccg ttattataaa ggtacaaata ccaagcacgg ttaatccaaa
     4321 ttgcataaat tcattttttt gtacactcat gcttttcatt agctctttaa ctgccattag
     4381 tactaagatg gcgataacaa cataaagtat atagtacgtc cgcattccgg aagcccatac
     4441 tgtgggagaa aatcccataa ttaaccttga acaaaatccc attatcatca aaataactaa
     4501 aaaaatagaa gttttcttat tgtcaaaact taaataaatt ccaacaatta aacatagtaa
     4561 taatgccaag actactaaag tagctatcca agtacctgga taaaaaagtg acagtttcgt
     4621 tcctaactct gtaaagagat tattcaggtt actactactc caaatcattg cccttttatt
     4681 tccattgacg taagtaaagc tttgacccat tgtattacca aaataaataa tcaggtttaa
     4741 gaaaaacggt aatgcagtca atatacgcac ataatagttt tgacatttcc tatatgttag
     4801 aaggaaaatt aagaaaaata gcaataaaaa taagatattc atgtctaaaa aaaacggctt
     4861 tccaaatgat gaaataccta aatctaactt attaagaaag ttgaaatttt tatattcagg
     4921 aaaccattta ttaatttcat gtgcagcacg taatgcattc cctggagttg tcaaagagaa
     4981 aattaattct gctaagctta ttattgaaaa tactgataac ttataattat atcgtccatt
     5041 gacaatcaaa tataaactaa ctatacttgt tagaacaaaa aaacaggcgt tgacttgttc
     5101 ttgattagtt aaaaatatca ataaaggaat acttacccaa tatatttttc tatttatttc
     5161 ttctcctctt aagagttgaa aaaacggata aaaagttagt aaactaaatg ctactggcca
     5221 ctgatagttc aaggtggttg ctctccaacc ggtttccccc attactgtca atggtattaa
     5281 acagtatata gagcaaacca ataataaatt tttaacattt attttctcaa aaacatactt
     5341 gcaaagtaat actacaattc ctaacataat cgtagaattt aataatttcc atagcataaa
     5401 gtgtttagaa aagaacatta agaatagttc tataataatt cttgaactcc atgtcttata
     5461 atgttctatc gaagcaccca aatagccaag ttcatctaat ctacttgcat aaactacgtc
     5521 atccgctctc atagtcggca tatacatatt aaagagtaaa aagatacccc aaatccctat
     5581 tagtaacaag atcagttctt tgttattcgt tatttttcta ttatgtttgt tcatttttat
     5641 actccttctt atatttccta atagagtata acatagcttt aagcattact taaagataac
     5701 ttaaaagtta tcttttaaat acttaattgt taaatacctt atttgatgca aaagaaatct
     5761 acgatagtca acaaaaaaat tttcattata tccagaaaaa tttttcttag ttaaggacct
     5821 atggattgca gtaatgcaga attcgtcgaa taaactgcca aagacataca atcatattgc
     5881 ttttaaaatt gacgaacaag atatagattc gtttgtctca aaaatccaaa tgttaggttt
     5941 aactgtcgaa ccaggacgtt ctcgtgtgaa aggcgaggct tcttcaattt acttttacga
     6001 ctatgataat cacctcttcg agttacatac cggtactctc caggaacgtt taaaatccta
     6061 caattccact acctgagact agtaatgact agtctctttt tatggcctct gatcccccca
     6121 ttgagcgaat actaataggg cttcgaattg atctgttcaa tctttaagca atatccctca
     6181 aatgagtttg gaaagtttag tgattcatca caaatactta aatacatgta gttatatata
     6241 gattgaggtt cttgtgagcg tatgctcgtg tgggttcaaa tcccatcgtc tgcatccatc
     6301 tttccaaaac aagcggctag gacatttttg tctcagccgc ttgttttcta gtttattctt
     6361 tcatcttgtc ggaccttttt tcttgggtat agacttttct catattctac cacaattttg
     6421 ggtttcccta acacatgata ggatctcaca acaaattgct ttctattatt ataataacct
     6481 gtaacgacta acttcatccc ttcgtctact tcagctagga agttcaaact atgggaagca
     6541 atcaaacaat tgaccccatt taaactgaaa cggatcaacg gtctggcact aacttgcaaa
     6601 gtacggatct tatctactgt tccatatttt ttctccataa ccttcacctc aataccatta
     6661 tacgaacata cattcccttt gtaaagaaga aactctgttt tatctttttg actttttgtc
     6721 tattaccttt cttaaaatca tattggcact tcctgcgaag atgaaggaca tgtactaaaa
     6781 aaaagaggca gcagtataga ttactgttgc ttctttacta atttaggttt acttgttaag
     6841 aaagattatt tacgcaaatg ttcttcaaca atttgctgag atttatcagc tgtatcttcc
     6901 acttcatctt ttgctttttc taacttttga atatattgtt tcaagcttga gttttcagca
     6961 gttgcttcgt ctaatttctt ttgtgtatct gttaactgag tttctacatt tgttttttct
     7021 tgtgtcagtg ttgcgatttg ttgattcaaa ccatttacag tattttgtaa ttctgaaagt
     7081 tttgcttgtg cttcttgtag tcttgctgtc caatctgcat tcagatgatt gatttcatca
     7141 tttttttggt tgatctcatc tagtttgaca ttgatttcat tttgtttgct ttgtagttgt
     7201 gcatccttgc tgactaattg actttctaag ttttgttttt ctgctgccga ttgatttttt
     7261 tggttttcta attcatttac ttttgtttgg tattcttgga catctgcctt tgaatcttgc
     7321 aatgtctgtt ctttttgtgc tagtcgagcg gtaagatctg taagcgcttg ctctttatct
     7381 gtcaatgtat gatcaagctg tgtcagattc tcattgattg tatcaatatc tgtctctcca
     7441 ccccacaaaa taatttcatt tgcgtaccgc gtgcctgtgt agccaattag acatagaaat
     7501 gcaacgacaa tcccaccagt aataaatttc tttttcattt gcatagctcc ttaatccaat
     7561 acttttacat gctaaaagta agtgatctca aacacaatgt caagcgtttt taaattacat
     7621 gtgtaatttt aatataaata ctatcaaata ctcatttaat cctccttttt tatctattta
     7681 aaaaatcttt tttatagaga aaaatgca
//
LOCUS       DAZULO010000111         7627 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000111 DAZULO010000000
VERSION     DAZULO010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.114"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(645..1991)
                     /gene="gor"
                     /locus_tag="ACG8OA_002496"
     CDS             complement(645..1991)
                     /gene="gor"
                     /locus_tag="ACG8OA_002496"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFS4977237.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2125..3378)
                     /locus_tag="ACG8OA_002497"
     CDS             complement(2125..3378)
                     /locus_tag="ACG8OA_002497"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFS4977238.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3384..4307)
                     /locus_tag="ACG8OA_002498"
     CDS             complement(3384..4307)
                     /locus_tag="ACG8OA_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFS4977239.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4528..7020)
                     /locus_tag="ACG8OA_002499"
     CDS             complement(4528..7020)
                     /locus_tag="ACG8OA_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFS4977240.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7027..7494)
                     /locus_tag="ACG8OA_002500"
     CDS             complement(7027..7494)
                     /locus_tag="ACG8OA_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFS4977241.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2104 a         1652 c         1225 g         2646 t
ORIGIN      
        1 aaaaattttg tgaaaatggc tttccacatt tttgcataaa tttggcttaa taccgagaaa
       61 ctggcttttg aacaccgttt atcagaggtt gtaaaatcag tgttttgtcc tgagtattag
      121 agaaacaaat ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgaagga
      181 cagctgattg aacactattt gttcggaaaa agtgactaac tatgacacaa tttttttcac
      241 agtccctttt ttcacttgat ttgattcgta ccagcgaact tgacgagaac tttcttggtt
      301 tggtaactat atggtattct tttaacatcc tcgtttgctt cttctaattt atttatacaa
      361 agtcacactt ttttaaacat ctgttcttcg atacatttct atttctttta aacatcgata
      421 ccaatatttt gaacttaaga gtgcatacca aatggattca tttactcttc gtcagttacg
      481 aagataatca aaatctccaa cttccctact tatttcgttt tgagaattat cgaagcaatc
      541 ttacataaat aactaaaaaa caaaaaacca gaaaatcagt atactgattt tctggtttta
      601 catggtttgt tcaataaaaa gctgttggaa ctgttaatat taaattatcg catcgttacg
      661 aattcttccg ctcctgttgg atggatggct actgtattgt caaagtcttc tttagttgca
      721 cccatcttga tagccacggc aaaaccttgc agcatctcat cgacaccaac accaatagca
      781 tgtaagccga cgattctttc ttcttttccg acacagatca atttcatttc gcatttttgg
      841 cgataatcat ttaatgcaaa gtacattggg gtaaagcgtg aacgataaac tttcaattca
      901 ttttcgccat atttttccaa tgcttcttct tctgtcaaac caattgttgc aattggtgga
      961 tgcgtaaaga cgactgtcgg aacaagttta tagtcaagat aagaatcttt ctttccatta
     1021 aataaacgtt ctgataaacg tctaccagct gcgattgcaa caggagtcag atctaattta
     1081 ccgatcacat caccaacagc aaaaatatgt tcatccgttg tattttggta tttatctact
     1141 ttgataaacc cttttttgtc caattccaca tttgtatttt ccagacctaa tttttcggta
     1201 ttcggtacac gtccaccggc aaaaagtaca caatcaccag ttaaggtttc accattttca
     1261 aatagcaccg tatattcgtc attgttttta gtaatttctt tagcaatatg atgtgcgtaa
     1321 gtttttattc cagcttcttg gtacatttgg atcaaattgt ccgaaagcat tttatcaaac
     1381 gttctaagtg gtcgttcttt tcgatatgcc cacatgactt cactacctaa accacgaaat
     1441 acacctgaaa gttcagccgc aatgtatcca gcacctaaaa cgattgctct cttaggtaac
     1501 tctgttaatt caaaaaatcc atttgaatca attgcgtatt ctccaccagg aatggtcatt
     1561 ttacttgcac ggcctccagt agcgatcaag attttaggag cacgataggt ttcaccgttt
     1621 acttcgatcg tttgactatc gataaaagta gcgtaacctt ttatagcctc gacatgattg
     1681 ctatctaatc ctctttgata agcaccgtgt agaaaatcaa tatatttttc tcgtctttct
     1741 actagaccgg cgaaatcaac attttttaaa tctgcttgga taccgtaact tgaggcatct
     1801 cgttcaattg tttccaaaat cgtacttgct tgccacataa cttttttagg tacacaaccg
     1861 acgttgacac atgttccgcc caattcattc gcttcaatca gcaaaacttt tgcgccatgc
     1921 atccctgctc tgtttgctga agcaataccg ccagagccgc caccaattac gatgtaatca
     1981 tatatttgca ttttgttcct ccttattgaa gagatcaatc aaaaccttta tttaacgatc
     2041 ctttttctac agaaaacaag tgtaacactt ttcttgaatt ttgtatgcga agatgcttaa
     2101 aacagggata tatcagcttt ttccttattt acgttttcgg atcatatctc caggtttcac
     2161 cggctgtgcg actggcatag ttaaaatcat cattggattt ggtgctcggt caatggattc
     2221 gccttcttca ttatgcataa cttcaacggt ttgactgaaa tgactgaatc ctggaccata
     2281 aaattcgatt tcatctccta cagaaaacaa gtttctctga cgtatcgtag caatctgtgt
     2341 tgcctcatca taagataata cttcaccgac aaatttatat actgggattt tacgacgttc
     2401 accgaataat tgttcgtttt cagttggtgt attgtagtaa aaacctgtag ctaattctcg
     2461 ttgtgctact ttccataact cgtcgatcca ttcttgcttg cagacatagt tttcaggatc
     2521 ttccatataa ctgtctacag ccgctttata gacatttgac accgtagata cataatggat
     2581 cgatttcatt cttccttcga tcttgaagct gtctacgccg ttttcaatca attctggaat
     2641 atgttcgatc attgacatat ccactgcact catcgagaat tcttcttcca cttcgcctgt
     2701 atcagttaaa ctgtttcgtt cttgaccaaa aggcatatcg aacaatccat acttccagcg
     2761 acaagattga gaacatccgc cacggtttgc atcccgcatc gacatatggt tggacaatgt
     2821 gcatcgacca gaataagaga tacacatggc accatggata aatgcttcga tttcggtatc
     2881 cgtattttta cgaatctctg ccacttcctc cattgaaact tcacgagcta aaacgacacg
     2941 ttccaaccct tcttctttcc agaattccaa tgtctcaaaa ttagttgctg aagcttgagt
     3001 ggataaatgg atcggcagac ctggggcttc tgtcgcacaa atttcgatca aagctggatc
     3061 agaaacaatc actgcagaaa taccgacatc acgcaaactg cggaaaaatt cccctgctcc
     3121 atcttcattt ccttcatggg tgaccatatt agcagcaacg tataccttcg catcatgttc
     3181 ttttgcaaaa gcaactcctt cagccatttc ttcataagta aaattacctg cacggcttct
     3241 taaaccatat gcattcccgc caatatatac tgcatctgct ccataatgga tcgcggtttt
     3301 taacttttcc agtgttcctg ctggcgctag gatttctggt cgtttcaatt ttctttttac
     3361 tgtcatttct ttcatcatac tccttatttg atagcagaag gatcaatata aaagaatcct
     3421 gtatctaatt cacggttttc aggatgcaat ttactgattt cgctagtcat tttttccgct
     3481 ttttcttgat cccagctacc ttctaaaatt tgtttttttg cttcggcgaa tagtcgagtg
     3541 atgtttacga atttttcgcc tggagtaaat aatccgtcca atttccatgt acagtaatga
     3601 tgctgataaa gttcattcaa ttcattcgag agattcagat cattgctggc aaaaatatgt
     3661 gtgccatgac tatcttcata aatagaataa tgtgtctctt cttttttagg ttccgagata
     3721 aaaagacctc tttcacgatc tttttgttcg tcttgtttcg tatagttata gtaattctgt
     3781 agcagtggac gtttggattg atggatgcat gtagcaccat acacaagggt ttctacagga
     3841 atatcgagtt tttcttccat cgccttcatc tcttcaaaag gaacttcacg agcaagtact
     3901 gctccaactg cgcctttttt cgcccagaaa ttgatttgac gtgcgctcgt tactaatgtt
     3961 tcagcatcat aaatatacgg tatacgcaat tcaggatttt ttttcatcgt ataaataata
     4021 cctgtatcac caactgtgat cacatctacg tttatttctt ttaaaaaagc caaatattca
     4081 ggtacatttt tcattttttc aggatgaatg atcccattta cagcaatatt cgctttttta
     4141 ccatacttgt gtgctaattc taccaattgt cgctgttctt ctcgtgtaaa tgaagcaggc
     4201 agacgcaatc caaattgctc ttcaccgaaa tatagtgtat ctgcacctgc agcaagcaac
     4261 tgttctgctt gttcaatagt ttctactgtg gttatgagtt cgatcataat tttctcctcc
     4321 tacaacttca taatagtagc atagctctaa aaactttcca atacttcttc tatttaaagt
     4381 cggatttttg aaaattattt cacaaaaaat caaaataaag ttcgatatat tctgtccaaa
     4441 tataaaaaga gattgtgaca aaaatcttgt cacaacctct taatcaagtc ttactattat
     4501 aaaaattcag ctacacaggc caagtaacta tagggaacct actgtttcct ttttaggtcc
     4561 gcgaacattc aatgtgatct taccttttga agcacctact gttacgtggt cacctaaacg
     4621 aatttctccg ccaagcaaag cctcgctcaa tcgatcttca atttcttttt gaagtgcgcg
     4681 acggattgga cgtgcaccat attcaggatc aaatccagct ttcccgatca catcgattgc
     4741 agatggtgtg attttcactt gaatatcttg ttctttcaat cgtttgataa tcgctttgct
     4801 catgatttgg acgattgtat ggatttcatc tttactcaat gaatggaaga cgacagtctc
     4861 atcgattcga ttcaagaatt caggtcggaa agctttcttt aactcttcta agatacgttt
     4921 ttgcatggct ttgtgatctt tagtaacatc ttggacattg aatccgacat ttttttcttc
     4981 tcgaatttgt gtcgctccga tatttgaagt cataatcatg attgtatttc taaagtccac
     5041 ttttctacct ttagaatctg ttaaatgacc atcatccaat acttgcagaa gtaaattgaa
     5101 aacgtctgga tgtgctttct ccacttcatc taagagaatc actgaatatg gtttttgacg
     5161 aattttttct gtcaactgtc caccttcatc ataccctacg tatcctggag gagacccgat
     5221 taatcggctt gtactgtatt tttccataaa ttctgacata tcaacacgga tcagggcatc
     5281 ttcacttcca aacatgactt ctgaaagtgc tttggccaat tctgtttttc cgacaccagt
     5341 tggtccaagg aacatgaatg aaccaattgg acgatcagga tctttcaatc cgcttcgtgc
     5401 acggcggatc gcacgtgaaa ctgctttgac agcctcatct tgaccaacga cacgttcatg
     5461 cagaagtcct tccagttcta acaagcgttc gctttctttt ttctccaact gctgtagagg
     5521 gacaccagtc cactccgata caactgtagc aacatcttct tctgttacac gatcagaata
     5581 accttgctcc tgttttgctt caattgcaga aactttttgc agctttttac gtagtgcttt
     5641 ttcttgtctg cgtaattgag cagcattttc aaagtcttgt ttttggatcg ctgcttcctt
     5701 ttcttgtact agttcatcga tttctagttt gatcacagtt gattttgtta agtgatcggt
     5761 ttgatccaaa cggactttcg ctgctgattc atcgatcaaa tcaattgctt tatcgggtag
     5821 ttgacgagaa gtgatataac gaacagaaag attgactgct gctcgtacag cttcatctgt
     5881 gatctccact ccatggtgct cttcatatct tgtacgtaat ccttgaagaa tcacttctgc
     5941 ttcttcagga gttggctcat ctacttgaat gcgagcaaaa cgtcgctcta atgcagaatc
     6001 tttttcgata tatttttgat attcatctag ggtcgttgct ccgatcgttt gtaattctcc
     6061 tcgagctaac gccggtttca agatattcga agcatcaatt gctccttctg ctccacctgc
     6121 accaattaag gtatgcaatt catcaatgaa taagattact tgaccatcat tatagatttc
     6181 gtcgatgacc tttttcattc gatcttcaaa ttcaccgcga tatttcgtac cagcaaccaa
     6241 agatcccata tcaagcatca tcaggcgctt ttcttgcatg tcttctggca cttcacctaa
     6301 aatgattttt tgagctaacc cttcagcaat ggctgttttg cctacaccag gttcacccac
     6361 cagtacaggg ttattctttg tccgtcggct aagaatctgg ataagccgtt tcacttctct
     6421 cgaacgacca acaactgggt ccagcttgtt ttcttttgct aatttcgtca aatcacgtgc
     6481 taatgaatct agcgtaggtg ttccttgcgg tgtttgccgt ttatcttgta catttacatt
     6541 tctacgacga ttcattcctt ttccagcaga atcaccagct agccccattt tttgtttcag
     6601 aacttgacgg gcttttgaaa ggctgatacc taagttgatc atgattcgag tagccatgat
     6661 actttcatct tttaataagc tcatcaaaat atgttctgtt ccaacttttt gagaaccagt
     6721 tttcttcact tcgctgctag ctaaagcaaa aacttgtttc attcttggtg aatatggaag
     6781 aaagactcct ttagggtaag aacggatacc accataacca atcagatgtt caatttcttc
     6841 ataaatttcc gtttcattca aatccatttc acgtaatact tttccagcaa ttccattttg
     6901 ctcgaccact aaggctagta aaacatgttc tgaactaact gattgatgtt tcaaccgttt
     6961 tgcttcttct tgtgcaattt ctaatactgc ttgggctctt tcagtaaaaa gttcttccat
     7021 ctaatctcac ttcccatctt cataacttaa acgttccaga aatgctcgga gaatccgagc
     7081 acgtgtatga tcttcatatt cattgaaatt caatgtgttt tttgcaattg cactcaacat
     7141 caagtttcct tctttttttg taattatttg gtcttcataa agtttttgaa taatagcaaa
     7201 agcatttttc tcactgattg tatcatcaaa taactgattt atctgctcaa gtaattgttt
     7261 tttatctgaa atccttactt ttgcaattcg tatataacca ccaccaccac gtttgctttc
     7321 tactagataa ccacgttgga tggtaaaacg ggtattaatc acatagttga tttgtgaagg
     7381 aacacagtta aagaggtctg ccatttcaat ccttcgaatc tcgatcattt cactattttc
     7441 cagaattttc tttatatatg attcaattaa atccgaggta ttctgatgtg ccatcttatt
     7501 cattcccttt gactaatatt gaccttaatt ataacgaatt tgataataga aagaaagaag
     7561 aacgcttttt gttaaataaa tcctttagaa tcaacaaaaa aaaatacgga atatactatt
     7621 ccgtatc
//
LOCUS       DAZULO010000112         7615 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000112 DAZULO010000000
VERSION     DAZULO010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.115"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..787)
                     /locus_tag="ACG8OA_002501"
     CDS             complement(59..787)
                     /locus_tag="ACG8OA_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS4977242.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(800..2080)
                     /locus_tag="ACG8OA_002502"
     CDS             complement(800..2080)
                     /locus_tag="ACG8OA_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS4977243.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2221..2823)
                     /locus_tag="ACG8OA_002503"
     CDS             complement(2221..2823)
                     /locus_tag="ACG8OA_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS4977244.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3068..3274
                     /locus_tag="ACG8OA_002504"
     CDS             3068..3274
                     /locus_tag="ACG8OA_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977245.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3586..4305
                     /locus_tag="ACG8OA_002505"
     CDS             3586..4305
                     /locus_tag="ACG8OA_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS4977246.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4386..5069)
                     /locus_tag="ACG8OA_002506"
     CDS             complement(4386..5069)
                     /locus_tag="ACG8OA_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFS4977247.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5287..7473
                     /locus_tag="ACG8OA_002507"
     CDS             5287..7473
                     /locus_tag="ACG8OA_002507"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFS4977248.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2727 a         1283 c         1435 g         2170 t
ORIGIN      
        1 agaaccgaag ggtttggatc ggctggtcat ttagtcggtc caaactattc ggttcttttt
       61 atgaaatcaa tttctttaat ccacggatag aggctgggat ggtctcgccg tttgccaacg
      121 tgacttctct ggttttttta ttaatcgacc gcacattttg tgtattgata acataggatt
      181 tatgacaacg gataaaattc cgatgcattt tctcgatttc tttgatcttc tcatagaatt
      241 gtaattgggc atgagtcaaa tggacttcca gtttatgtgg cgctcctagc gaagtagaaa
      301 aatattggat gtctttgatc ggtaaaaatt tgatctcatc attaaactct attttgaaga
      361 tatctttttg gatttgggac atttgaaaat cttcgaaaat tttgctcagg atatgctcga
      421 cattttcttt taaatcataa agatcttctt tgacgatata atcagtcggt tcgatgttgc
      481 tttttaagat tttcatcgac atttccgtat gggaagtcac atagattatt tttcctaaag
      541 gatctaattc acggatcaat ttaccgatcg ctacaccatc catttcactg tcttctagtt
      601 cgatatctaa aaagtaaata ccaaaagaag aagttctgat cttggctgca ttgagaagtt
      661 cttccggatc agaggtagaa atatggacat aactgtctaa gttttcaatc atgattctat
      721 ttttgatcac cgttgataaa acttctaatt gttttggatc gtcttcacaa atataaatgg
      781 gaatcatatc attactcctt tattttattt gtatataaat cgtttgagaa aaacgctggt
      841 tttcaattga tgtttcaagg aaaagataag gattggtctt tgtcaattca tccaagataa
      901 acaagccaag gccttcgttt tttggatttg tttttgttga gaaccctttt tgtttcaact
      961 ggtttaacgg taatgtattt gttgttgtat tgatcacacg aatcatcacg gaatgattct
     1021 tcttgagtat ggtcaattcg atttttttct tctttgcagc actagctcct tcaatcgcat
     1081 tgtccaagat aataccaagc atacgtacaa ggtttaccgt atgtttcgta tccagttcga
     1141 ttggctcagg aaaatgaagc tggacgatca accctttttc ctgtgccatc atgagtttta
     1201 atgacagtaa actacggatc tcaggaactt tcatattatc caacatactt agattggccg
     1261 gaaaaaggtt catcatctgg cgtgtaggta atatcgtttg atcataataa gaccgtaatc
     1321 cttctaagtc gccttctgaa atatagctgt gaagagaaaa aagcagattt tggtaatcat
     1381 gtttgaattc tctcaagtct tgatagtttt tttctagatc tttgatataa tcctgttgca
     1441 tcctgacttg ttgctctaat aactgatcag ctaatttctt attagagatt ttccaataat
     1501 aaaccaagat gatcaggcca acacacagat atagaatgaa aaaactcgta ttcaaagcta
     1561 agacatcagg tgtttcacca gaaaaacggg tataaaaaat aatgatatag tatgtaaata
     1621 atatcaatgt cccgatcgta ccgatcatac ggcggtccac agtgtgaaac caagtctcta
     1681 acgatttttt taaacaaaaa gctaataaag cagataaaaa agcggataag acaatgtgaa
     1741 aatactgcaa tgaatctcct ggttcaataa ccgaagattt caaaacaaaa aaatcgtcga
     1801 tgcttgttaa gtgatctcct aaaagcgacg tcaataaccc aaaaggaagc gcgtaaacca
     1861 attgttgacg cgaagtcgaa aagaagaaaa aagtaagcat cagcattcct atagcaatta
     1921 gagcagtaaa aatccctaaa atcgaataga cccatccaat caaaatacaa gaaatcagaa
     1981 tgactcccgc aaacagatat ttgtatcgga tgtgatggtc aatcaataag atacaaattg
     2041 tgtaaatcaa atattgcaac gctatggacc ataaaaacat aaagtacagc tcccattgat
     2101 ttttaccaaa ttatagcaca attagcgtat tattttttta atttttcagg gattttgaat
     2161 tcagaactca taccaaagca gcagccagat acaaaaaagt cagataagtt agttaattgt
     2221 ttaatgattg tttttttcat gaaagattcc tccaaaattc atcaagtagt ttgtaatagg
     2281 aagcaacgta gctatttcta aaacaatccc aagtagcaaa taggtagaaa agtaagaagc
     2341 tgagaaaaat aaagtaatga gagaaaaaat tagaaacatg ctgatcgcct tctttaataa
     2401 tgggggacgt ttctcgggtt caatgggatt gattgctgta cctcttgatc catagcgaag
     2461 cagtaacaaa aagcagctga ttaacaaaaa gaccatcatt gggaaggaga tagtcaattg
     2521 cagatgtttc atcaccaagg gaacgcccca taaataaagt agtgtccaca gcagacaaac
     2581 gaattcagag tcggaatgca atccataagc atatctgcgg atgcagatat aagaaagaaa
     2641 ggtgattcct gtttccgccc atgagcctgt caccagagaa aagactagaa cgaccgctag
     2701 ttttccaaga ttattcaata aaacttctaa ggcaaatttt acttggacat acaccatatc
     2761 ttcttccgat ttgtctccgc tgaataaggc ttctaataat ttgttcacaa ctttatcttt
     2821 catctcatac gtacctccac cacaaatttt atgctaaaaa aaataaaaaa cgtcttttgt
     2881 ttcataaaaa gtggttttga catcaaaacc gtcatgatct cctaaaaaaa tcttcgtttt
     2941 ccaagaacct taccaaaaaa agtgacagtt ttgatacgaa aagtactttt tatgacaagt
     3001 ttgtgctttt tcgagtgatt tatgttaaaa aattactgac cttataaaag gtgatggagg
     3061 gattcttatg gaaaatgaac aattgtttga agtcggcgaa acatatgcat ttaaatctga
     3121 tttatttgaa tcaccaataa aaggaaaaat agaaaaaatc tatgaaaata gtgtgatgat
     3181 acgtgtcatg aaatgcgcag tagaagacaa tgataccgct cataatctgc actggcagtt
     3241 agttgtacgc aaatcaagta caatagaaga ataaaaacag ataaaatgtt gagagcataa
     3301 agaggttgtg agtctcccgt ttagctctga gttacaagga ataatgttaa aaaacagctt
     3361 ttcatgtttt atgaatattg ccttgtgacc gaggtgttgg caggagaata ctgtttattt
     3421 gaaaaaaggg ctgcgacaag acttttgtca cagcctttat tttcatgaat aggagttact
     3481 tttggctact tttagagaag tagcttttaa ggagcagata aagtcaaggc ttttatttct
     3541 ataaatgaag tagtaaaatg aagaacagat tttattacag gaaggatgcc gataattgtg
     3601 atcattgttg caggaacgat cggtgcaggg aaaagcacac taactgaaat gcttgcccaa
     3661 gatttagaaa caaaaccttt ttatgagaac gtggaagata atgaagtatt gccgctattt
     3721 tattctaatc cagaaaagta tactttttta ttacagattt tctttttgaa caaacgcttt
     3781 ttggcaatca aagatgcttt cagccatgat gacaacgtac tagaccgctc catctacgaa
     3841 gatagtatgc tgtttcattt gaatgcagat ttagggcgtg tttctgaagt agaagtaaag
     3901 cagtatgaag gtcttttaga aacgatgctg aaggagttag aagaaatttc gccacaaaag
     3961 aaaccagatc tactggttta tattcgtgta tcctttgaaa ctatgttagc gagaatcaaa
     4021 aaaagaggcc gggaatatga acagctagaa caagatcctg aactctattc ctactataaa
     4081 gaactcaatc gacgatacga agagtggtat gaacagtttg atatatgtcc caaaatagtg
     4141 atcgatgggg acaaatatga tttcgtagcc gatccggcat gtggagaaca aatcgtacaa
     4201 gaaatcaaga tgcgggcaaa gaaaatgaca gaagaggcag atgcccagtc gatccgctac
     4261 actacaaata acccgaaagc aaagggaatc tttacaaata aataatataa aaactaagca
     4321 aattgctcac tcgcagatat ttctctgtga gcagcatctt gcttagtttt tttgattcat
     4381 tgagatcaaa tagtgaaccg attcgtatga taaaattgcc ataaatgaaa tgtctgagtg
     4441 agggccatga tcaaaaataa aatggaaacg atcaaaatcg aatgggcgcc aaacagatga
     4501 tgacagaaaa caaggacaat agctcctgcg ataaaggcaa gaacgacact tagatagtgg
     4561 aaaaatcgat caaaatctgc tttttcttta gaaatacaat aatcatataa gttcgttgct
     4621 acccctttta aatttccagt tgtaaataaa ttagtgtagg cacgcccgtt gattttatca
     4681 aaagctaccc actggatcga tgcagtaaaa gcaatcaaaa tcgttactaa tgcgggatgc
     4741 tcatggacgg gtgtcaaact aacaaagaaa aaaatcactg cttcataata tagaacaaac
     4801 aagcgccaga aatgtttttc tttttttcta aaataatcaa ttagatactt tgttagaaga
     4861 atccctaaga aaaagaaaag ggtagacaat acttttttac caacacttag ccattctccg
     4921 tcaaatgctt ggatgatcga tagaatgata ttgccagttt gagcagaggc aaaagcacca
     4981 tattggatat aagtataact gtccatggct cctccgataa atgtcagcaa aagtcctatt
     5041 gtacgcccct cgtgaaagga agtctgcatt tttttcatca ccttttatga atatttttgt
     5101 cttccttatc ttagcataaa cgattgaaaa aaaggaaaaa ttttcagaaa actaaaatca
     5161 tatcgcttcc cattttcatt tttagaaagg tctgttagta taaagaaaaa atagacaacg
     5221 aaactttaaa taaccaactt gaaataaaca aattcaaaga agaaagatgg gaaggaaatc
     5281 aatcagttga atgaaaaaga aattctccga ttacaaaaag aattgaaacg ctgggagaac
     5341 aagtataaaa agcttcaaca agtctttcgg ctacaagaag aaatcattgc tacctatgaa
     5401 gcggcttatg gcgaagcacc cggacttatt gaggaagaag tacaagtaaa agaaagtgaa
     5461 ggaaacagct ctttctttaa aatgatggag aaacgttcct ataatcgctt caatcaagaa
     5521 caaaaagcag ctatcacata tgatatgaag aaacatcttc gaatcatagc aggagctggg
     5581 agcggcaaaa cgcaaacaat ctgtgcaaaa gctgtttatc tgatgacgca aaaacaagta
     5641 gacgaagaac gcatactaat gatcactttt acaaggaacg ctgcaaatga actaaaaaaa
     5701 cgagtagata atttcagcca gagaaaaacc gctgtccata tcggaacatt ccatagtatc
     5761 ttttttagga tatacaatga aatttgtcga aaatttcctg aagtagcaat gcaaggaata
     5821 caaggagact tttcaaagga tactgcacaa aaagtgaatg ctgttttgca acagctgata
     5881 cgtaaatata atctttatat atttgatcaa tatggagaaa aaacgattgc ttctcggtta
     5941 gactattggc agaatatgaa cctttcagta gaagaaatgg ttcagttagt taaagaaaaa
     6001 tatgattcca ttgataagaa tgcacgacag ccgatatcag aacgactgta tggattgctc
     6061 acggaactcc aagaacaaaa aagaagccag caattgctag agttcaatga tgtactccaa
     6121 aatctgaaaa gcgctttgga gaatgaggag atccagcatt atatcggcca aaaatatgat
     6181 tatctattca ttgatgaatt tcaagatact aatccacttc aatgggaaat cgtgaaacgg
     6241 atgacaaagg aaaacaggat caagttgatt gttgttggag atgatgatca gagtatctat
     6301 ggattcagag gatcagagcc agcatacatc aagaactttg aaaaagaata tcctaccaaa
     6361 actcttttct tattgactaa ttatcgttct cgggcagcta ttgtccaagg agcaaataga
     6421 ttgatcagct ataataaaaa tgatcggata ccaaaggcga tgattccagc acaaaaagaa
     6481 gcaggtgtta tggaagccta tttgttttct gatactaaag cagaaagtca gtggctaatc
     6541 aaacaaattc agacatttat tcaaaaaaat gggaaatata aggaatcaat catattatac
     6601 cgatcagctt cacaaacgca gcagcttgtc caatctctgc tgaaaacgaa tattcctttt
     6661 gtactggaag cagatagtcc atatgaaggt atctttggta tcaagtcttt ccaacatttc
     6721 tatcaaaaaa ttgttcattg gcaaacagca ctgaatctgc aaaagaaaaa tcaggcctac
     6781 cagcaattgc tccggcagct gatggcagac tgctacctga aaaaaagtga atgcgatcaa
     6841 tacttttctc ctaaatatca aaatatagcc gttgcagatt atattttgtc gataagcccc
     6901 aatttaaaat cgaaatctgc agaaatccga gagtttgagg aagcgttgcg taaaacagac
     6961 cagcatcaag aaatctattc gcttgttcaa agctatcttc atctgccacg tatagctaag
     7021 gaaatcgacc agagtgatca agaatggata cgagaagaag taaagcagca tgctactttc
     7081 agaagactcc aagccttatc gcaagaaacc caagcgacag ctaaagaatt aaaaacccgc
     7141 ttgattgctt acagacaagg aaaacttgat gcactatgta tccagagcat tcacaaaagt
     7201 aaaggtctta gttaccgaaa tgtctttttg attggttgta acgaaggaag tttgccttat
     7261 aacggagcga cagaaaagaa agtaatcgac tttaatgaaa taaaagcaga accagtgacg
     7321 acgatagaag aagaacgaag actattttat gttgcgatga ctagagcaag aaatcgtctg
     7381 tatctatgtg tacctcaaat gaaaaattca agaaaattga aagtatcgag atttatcaaa
     7441 gaaaccggga gtcgtgtgaa aaaaggaaag tgatagaaga gagggtgtga cagaagtggt
     7501 cagtttcaag aaagaagaag gaatgtacga aaattgcttc tcaaattttt gtgaaatttc
     7561 agcttatttt cgaagaagct atttctggaa cacagtttat tcggaaaagg gaggt
//
LOCUS       DAZULO010000113         7601 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000113 DAZULO010000000
VERSION     DAZULO010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.116"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..428)
                     /locus_tag="ACG8OA_002508"
     CDS             complement(138..428)
                     /locus_tag="ACG8OA_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977249.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="ACG8OA_002509"
     CDS             complement(546..806)
                     /locus_tag="ACG8OA_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977250.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="ACG8OA_002510"
     CDS             complement(943..1356)
                     /locus_tag="ACG8OA_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFS4977251.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="ACG8OA_002511"
     CDS             complement(1812..2297)
                     /locus_tag="ACG8OA_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977252.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="ACG8OA_002512"
     CDS             complement(2436..2651)
                     /locus_tag="ACG8OA_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFS4977253.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="ACG8OA_002513"
     CDS             2875..3675
                     /locus_tag="ACG8OA_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4977254.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="ACG8OA_002514"
     CDS             3694..5562
                     /locus_tag="ACG8OA_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS4977255.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="ACG8OA_002515"
     CDS             5555..6046
                     /locus_tag="ACG8OA_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFS4977256.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /locus_tag="ACG8OA_002516"
     CDS             6034..6588
                     /locus_tag="ACG8OA_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFS4977257.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..7601
                     /locus_tag="ACG8OA_002517"
     CDS             6606..7601
                     /locus_tag="ACG8OA_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFS4977258.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2709 a         1213 c         1442 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg a
//
LOCUS       DAZULO010000114         7464 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000114 DAZULO010000000
VERSION     DAZULO010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.117"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..1175
                     /locus_tag="ACG8OA_002518"
     CDS             join(34..262,262..1175)
                     /locus_tag="ACG8OA_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4977259.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1410..1862
                     /locus_tag="ACG8OA_002519"
     CDS             1410..1862
                     /locus_tag="ACG8OA_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977260.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1876..2565
                     /locus_tag="ACG8OA_002520"
     CDS             1876..2565
                     /locus_tag="ACG8OA_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977261.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2690..5041
                     /locus_tag="ACG8OA_002521"
     CDS             2690..5041
                     /locus_tag="ACG8OA_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFS4977262.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5121..5516
                     /locus_tag="ACG8OA_002522"
     CDS             5121..5516
                     /locus_tag="ACG8OA_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977263.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5841..6458
                     /locus_tag="ACG8OA_002523"
     CDS             5841..6458
                     /locus_tag="ACG8OA_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4977264.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6499..6798
                     /locus_tag="ACG8OA_002524"
     CDS             6499..6798
                     /locus_tag="ACG8OA_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFS4977265.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6832..6999
                     /locus_tag="ACG8OA_002525"
     CDS             6832..6999
                     /locus_tag="ACG8OA_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4977266.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7044..>7464)
                     /locus_tag="ACG8OA_002526"
     CDS             complement(7044..>7464)
                     /locus_tag="ACG8OA_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4977267.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2574 a         1124 c         1495 g         2271 t
ORIGIN      
        1 gaatctaact actaagaaaa ggacgtgtcc acgatggcaa aagccaagaa aaaatttgat
       61 gaagacttta agaagatgat cttagattta aaccagtctg gccaatcggt cgaagaactg
      121 gcagcgcaat atggtatcgc tacacaaacg atttatcgtt ggaagaagct tcatacaaag
      181 aatgtagcaa ccggcatgac tgaggcagaa atcctagcga tgaaaaagga aatggcccgc
      241 atgcaggagg aaaacactat cctaaaaaag gctttaacca tattcgctca aaagtaaaaa
      301 tgagtgatgt cttcgaattt attcaatcaa atgttcatga tcatgacgta aaacaaatgt
      361 gtaccgtttt ggaagcaccg aaatccagct actatgactg gaagaaaaag aaaccttcca
      421 agcgccaatc agaaaacaaa cgcttaaacc gcttgatttc aggaatctac tttgaaaaca
      481 agggtatcta tggtgcgcct aaaattcata agattcttgt tggccgtggc gagaccttgt
      541 ccttaaagaa agtacaaata ctgatgagaa aattaggctt gcgttcagtc accctcaaga
      601 agtacaagcc tggcaaacaa gcgaaagtta aaagtgaagg acgtaaaaac ctgttaaacc
      661 aagattttac cactacgtcg atcaatcaaa aatgggtgac agatattacc tatattcata
      721 ccctaaaaga tggctggacc tacctctcca ctattcaaga cctccacaca aagaaagtca
      781 tcggttggaa gtttggcaag cagatgacga aagaattggt tatcgaaacc cttgacaatg
      841 cgctcttaaa ccaaacaccc tccgagaact tgatcatcca ctcagatttg ggttctcagt
      901 acactagcga agcctatgag gcaaagctga atgaattgaa cattcggcac tccttcagtc
      961 gtaagggctg tccctacgat aatgccggta tcgaatcgtt tcacgcctca ctcaaaaaag
     1021 aagaagtcta tccatcaaaa gcgtatgaaa actttgaagc tgcttattta gcgttatttc
     1081 aatacattga aggattttac aatcgcaagc gaatccatag tagtatcaac tatttaacac
     1141 caaatcagat ggaggagttg gcattgcaag cttagttcct agctaccact tacatttttt
     1201 tggcgagatt tccacagggt ttcaacttca tcgcttcgat cagcggtcaa acggttttgg
     1261 tttgaccgct ggtcgaaacg atgagatact cacccgctgg aatctcgaaa aaattctcac
     1321 aaatcgtgtc cgaggtattg actcaaatcc accaattatc ttatcgtaga tatatattat
     1381 aaatttaata agaaagcgag gtgagaatcg tgaaaaaaac gatactaagt attttttcag
     1441 tatttacaat attaattggg ggctattttc ttttgaattt agattctgtt aattcaaacg
     1501 ctcaaataaa agatcaaaga gtagctgaac ataataataa aaaaatgagt ttgaccaaaa
     1561 atatctattc tatgtcagat gaagaattgt ataaagaatt tggtgattta tccggggaac
     1621 agttagaaga aagtattcca ggaattagtg actcagataa aattgttgtc ttgtctgctg
     1681 gtgggcaatt aatgggagat ggcaaatccg aattagttag ttataaacaa aatcacagac
     1741 agtttattga tgttaatact gattccaacg ctatttcgat taaggatcta aaagagcttt
     1801 taaatagtaa aagaaatgaa ttaaagcaga taaaagaaac taattcagtg gaattagagt
     1861 aaaggaagat tcattatgaa aaagattttt ggtgtttctg ttgggctact tatgatgtta
     1921 acatttggaa atgcagtata tgctgctgag aaagaagtta tttcagatcc agagcagtac
     1981 gcctcacgat actttgctga ttttgaagaa gaagataaag tacttgcatt gccagaaggt
     2041 ggttttttaa aaggggaagc taaaattgta tctatggata atccagatat aattcaaggt
     2101 tcatataatt cagaaactga tcctgcttca gtttcagtga cagaagcaaa aaaaattgtt
     2161 gctgaaaaat tcaaaaatga tattgaaact actccaatta tgcctcgagg agcatttcca
     2221 cccgatgttg gtactagcgg acaccttctt gtactagcag gaggaggaag ctataaatca
     2281 cagccattta gtggtgcggg atggcgtttt ggaggtatgg gtgtaaaacc agctgatggt
     2341 acaggcaact ttttacgttg gaaatcctac attgacgatg gaagagtagg aaatgtaaat
     2401 caagccaacg caactaaatc tggttctatt caaggaatgg cattgtatgc tcgtgaagga
     2461 ttcaaatact ttgcaccaaa cgggcctggt tggacaatga atataggtat ttattacact
     2521 tataatcctg ttgcaggtac gtattatgtt gttcaaaata tttaataaaa cagcacaaag
     2581 agatttcttt gtgctgtttt attattttta gttgttgaca aacactaaaa tataaaatat
     2641 aatcaacaca taaacgaacg tatgttcggc aagaaagcag gggattttta tggatgagat
     2701 ggttttaagt actcagaaat ggctaaacaa gaaatacagt aatgttacag ggtttgataa
     2761 ggttccagaa aatgggcgaa cagggtggcc tacaatctat ggacttattg aaggactaca
     2821 agtagaactt ggtattacta atctagtagc taattttgga cctaccactg aaaaaatgta
     2881 cgataatcag gttactccta aatggggcaa aaatctacct aaaaatattg tatttcttat
     2941 tcaaggtgcc ttttggtgta aggggattaa tccaggtggc ttcgatgggg tatatactcc
     3001 atatttagat actgctgtaa aagagttgca gactgatgca gggtttaatg gaagcgctgt
     3061 aactggagtt ttggatgcga aatgggccaa agcgttattt gatatgagtg cttttgttct
     3121 tgttcccggc ggagatagta aaattagaaa aatgcagcaa tggttaaatg taaattattt
     3181 agaatacacg ggaataatgc cttgtgatgg tatttatcaa agaaattcaa atcaagcatt
     3241 aatttttgca ttacaagctg aactaggata ttctcccagt gaagcaacag ggtcttatgg
     3301 taatgggaca actagtaaaa cttatcctgt gtctgaaggt aactcgggaa actatgtaag
     3361 aataattcaa tatggattat atgttaatgg ttattttgaa gacggtacat tcgatggtat
     3421 tttcactaaa tatatgggtt tagaagtact ggcattccga aaatttatgg tattgcctga
     3481 atatactgaa atagcagatg ttgttgtaat aaaaggacta ttgtctagcg ctggagatat
     3541 tagaagatct gcagatggcg cagatacttc tacgcaatta actagatctc aaatccaaac
     3601 attggttgat aatgatataa aaatagttgg tagatatctt actggtactg ttggaattgg
     3661 aaaagatgaa cgaaataagt atctaactac agaggaattg aataatattt tttctaaaaa
     3721 tttatctgtg tttcctatct atcaagatgg aggtgcagca ttagcttatt ttacttatga
     3781 tcgtggctta tcagatggta agaaggctat agatgcagca aaaaatctaa atataccatt
     3841 aacaacagtt atttattttg ctgttgatct agacatgtta ggggaagaaa ttcttgcata
     3901 tgctggagaa tattttaagg gggtatctgc tgtaatgtca tatagcgggt atcaaacggg
     3961 tgtttacgga acaagaaatg tttgttctca aataatcaat aacgggtatg cttcgtttgc
     4021 atttgtttca gatatgtcga ctggttatag tgggaattta ggttttccta tgcctagaca
     4081 atggtctttt gatcaatttg ttgaatttac cataggaagt ggcaacggag ctgttggtat
     4141 cgatttaatc gctacatcgg gtagagatag cggatttaat gagttatcaa atgatactaa
     4201 taatgataat tacattgcca aatacaatca gaaggttata aatcagatgg tacgtgcgta
     4261 tcaatatcta tcacaagctg gtcttgataa tccttggaac cctcatttga cattttatag
     4321 atatgtaaat tattctggtt tgtcatggga catcatttct tctcctgtaa cagagcatga
     4381 tagaaaaatt tatgatgaat ataggaataa attaactaat agcgagggcc tgtataatta
     4441 ttttatagat cctaatactg gttcaataat aggtttgcct cacttaattg tgacgttaca
     4501 atcacagatg tttctagccg acacaatcaa tgatagtgtt tctgactttg caggttggtt
     4561 aggagattta atgacttgct ggggtgaagt taagaaatta ggaatacaaa tggagcaagg
     4621 tgtatttgca ttagtaggta cggctgcagg tggaacattt agtctagaag atttatacca
     4681 agatgttgac gcggtaaatg tttatacctt agcaaaaaga aattctgatt caacagcttt
     4741 atcggcttta aaaacgtact attgtgataa gggtgctatt gatagattta atcaatttgt
     4801 taagaacaga tttggttctt cagataaaat cgcttcagta acaaatagtt tattagaaaa
     4861 tgacgaaaca gttgatatct taggtgtagg gataatttca tttttcattg ataaaatagt
     4921 taataattca cttgggacag attttactta tcttgatttt attggagatg aggggaaagt
     4981 tgcaaatgga ttttctgaaa aaattattca atttcaagaa aatagaagta aacttaatta
     5041 agaatcgttt actatttaaa cagtgtcctt ttccttttta aatatatacg ataaaataag
     5101 tatatgaatt gtaaaaggac atgatgatta tggaagaaat agaatggaat aatctgactc
     5161 aaaataataa aaataattct ggatgtggaa ttatctttat gtttgttata gttttgggaa
     5221 tagtagtact tttaataact gtcaagatag tagattactt tgcgaatact cctgaagaac
     5281 aaatacagag aaaacttcct gagtttgaaa gagtaccacc ttatgatcca agagataatt
     5341 catttcaagt aaaaaaatta aaagagtctc caaatgaaat gtggtttgaa gttacagttg
     5401 aatcaagcga cttcaaagaa aaaaagaaag aatacaatta tattgtaaag gcaaaagtat
     5461 caaaaagatt tatttttaac cctaaattag aaatatatag tgttgtaata gaataatagt
     5521 gttaaaaaaa ttgtaaaagt taaagaagtc aaattctgat gtgaaaaata aaatatacca
     5581 tggaagacat aaaagaaaaa ctggataact agtttattta atggcagacc gttaaggcct
     5641 gccattttta tgtattttag cggaataaat ataatcatgc gggagtgtaa acttactgtt
     5701 tcaaaaagta tactttttga catgccttga ttttctttgt ttatgtatgg cataataacc
     5761 acataataca atttgaaacg tttctgattt aatctgattg ttttgaaaca attttagtga
     5821 aaaaagggta gataaaaacg atgagaaaat ttttttatat ccgggtcagt agttatgatc
     5881 aatcgatcga acgacaactg atttccgcga aggaattagg aatccctgaa gaatatatat
     5941 ttgtagaaaa agccagcggc aaagatttta aacgccctga atatcaatta atgaaacgga
     6001 tgtttcgaaa aggagattta ctctatctac aatcattaga tagactaggc agaaataaac
     6061 aaatgatttt agaagaatgg cgagaattaa ctaaggaaaa gcagattgat atttctgtgt
     6121 tagacatgcc tctgttagat actgctagtt acaagcaact agatgggtta gagacgctta
     6181 tctctgactt aattctgcaa ttactcagct atatggccga agacgagcgg aaacgaatta
     6241 atagccggca aagagaaggc gtagaagctg cgaaacaaaa gggtgtaaaa ttcggcagaa
     6301 agaaaattgc tttacctcct gaatttcctg cgatctattc tgactggaaa gcaggaaaga
     6361 taactgcagt ctatgctatg gatcaattgg gcatgaaacg aaataccttt tatcgacggg
     6421 tgaaagaata tgaacatacc ttgcaccact tttcttgata gatataacag ctattagacg
     6481 ggaagggaga gtttgaaaat ggacttaacg aatcaaaggg tcgtttttac tggaacgctg
     6541 cagcaaatga cacgaaccca agccatcggt ttggttcact ctttaaacgg ccattgtcaa
     6601 tcaagtgtta cttcaaaaac aaatttcctg gtttgtggtc agtattctaa gtcgttgttt
     6661 gatgattctc tctatacaaa aaaggaacaa accgcgcggg acttgattgc gttaggtcat
     6721 gagattacca ttttatcaga agtcgaattt tatgcgttaa ttagtcaaca gttgaaagaa
     6781 tggcaacggt atttataatc gttagtaaaa tttacaagga ggaaaatttt tatgaagcga
     6841 aaatcttatg atcgttgggg cattgaaata actagtttta atcgaaatat tgcttttcac
     6901 gtggagtgtg actgtgggga attagcaaaa agtgtttatc aaaaaaatta cttttactgt
     6961 cctaaatgcc atcgccgata cggggtacat ggaacataga aacgaaatag tgcggtagaa
     7021 aagcgaatga atgtagtata tttctacaaa tcattcgctt tctttatttt ataaatagtt
     7081 ggtcgagaga tcccgttttc ttttgcaatc tctgcaactg gaatgccttg ctttaacatg
     7141 tccaccacgg ttttaaaaac taagcgtttt tgcggatctg ttgagttaat agaatactcg
     7201 attggtcgtc ccttatatcg gccggctttt tgagccaata atactccttg ccgctgacgt
     7261 tcacgaattt tctcacgctc gttttgtgca atataactta gcagtgataa aaacatatcg
     7321 aacatagcgg tatccaggag ttcatttcca gtattaaaat ctaaaaactg tgcatctaat
     7381 atttttaacg ctactttttt ctgtttcata aaagcaactg tattttttat atcttcataa
     7441 tctcttccta aacgatctag tgat
//
LOCUS       DAZULO010000115         7325 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000115 DAZULO010000000
VERSION     DAZULO010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.118"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..2721
                     /gene="clpB"
                     /locus_tag="ACG8OA_002527"
     CDS             112..2721
                     /gene="clpB"
                     /locus_tag="ACG8OA_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFS4977268.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2872..3558)
                     /locus_tag="ACG8OA_002528"
     CDS             complement(2872..3558)
                     /locus_tag="ACG8OA_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977269.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3536..4030)
                     /locus_tag="ACG8OA_002529"
     CDS             complement(3536..4030)
                     /locus_tag="ACG8OA_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS4977270.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4050..5270)
                     /locus_tag="ACG8OA_002530"
     CDS             complement(4050..5270)
                     /locus_tag="ACG8OA_002530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4977271.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5417..7252
                     /gene="lepA"
                     /locus_tag="ACG8OA_002531"
     CDS             5417..7252
                     /gene="lepA"
                     /locus_tag="ACG8OA_002531"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFS4977272.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT         2412 a         1400 c         1503 g         2010 t
ORIGIN      
        1 gtcaaaaaac tatgtgtata ttttgaccaa aattgaccaa agcgatataa tagtttttgt
       61 aaagatcaaa gaaataagtg atcttacctt taattaggag ggtatacctg tatgaatatt
      121 gagaaaatga ctacgacgct tcaagaagcg attgctgaag ctcaacagat tgcggtcact
      181 cgaaaacatc aagatatcga cattgcccat gtatggaaaa tctttttaca atctaatcat
      241 tttggtcgaa atttctatac ggatgccgga ttggatgtcg aatcttttga acatgaaatt
      301 gatcgtctac tggatgagta tccagttgtt tctggtggaa acgtccaata tggacaaaac
      361 ctcagccaga atttctttcg attattgaat gaagcagacc aaatacgtga aagcttcggc
      421 gacgagtttc tttcaacgga agtcgttatt ttagcgttga tgaaattaaa aaattatccg
      481 ttaactgttt atttgaataa aaacggatta tctgaaaaag aattgcgtaa aaacatcgaa
      541 gagatgagag gaggagaaag agtgacctca caaaaccaag aagaacaata caaagcatta
      601 gaaaaatacg gcgttgattt agtacagcaa gtcaaaagcg gaaaaatgga tccaatcatc
      661 ggtcgtgatg aagaaatccg tgatgtcatc cgtattcttt cacgaaaaac aaaaaataat
      721 cctgttctga taggagaacc cggtgtaggg aaaacggcaa tcgttgaagg gcttgctcaa
      781 cgtatcgtac gcaaagatgt gcctgaaaat ttaaaagata aaacgatttt ttcattagat
      841 atgggtgcat tgatcgctgg agcaaaattc cgtggtgaat ttgaagaaag actaaaagca
      901 gtcttgaaag aagtcaaaaa aagtgatgga cgtatcctct tgttcatcga tgaaatccac
      961 aatatcgtag gggcaggaaa aacagaagga agtatggatg ccggaaatct gttgaaacca
     1021 atgcttgctc gcggtgaatt acatttgatc ggtgccacaa ctttagatga gtaccgtcaa
     1081 tatatggaaa aagacaaagc gttggaacgt cgctttcaaa aggtattagt caaagaacca
     1141 acagtggaag atacgatcag tattctgcgt ggattaaaag aacgttttga gatccaccat
     1201 ggtgttaaca tccatgacaa cgcattagta gcagcagcta ctttatctga ccgttatatt
     1261 actgatcggt tcttaccgga caaagcaatc gatttgatcg atgaagccag tgcaacgatt
     1321 cgggtagaaa tgaactctat gccaacggaa ttagatcaag ttactagacg tttgatgcag
     1381 ttggaaatcg aagaagctgc actgaaaaaa gaatcagatg atgcaagtaa aaaacgcttg
     1441 aaaaatttgc aagaagaatt agctgaactt cgtgaagaag caaatgcgat gaagatgcag
     1501 tgggaaacag aaaaagaaga agtcaattct gtttcagcaa aacgtgctga aatcgataaa
     1561 gcaaaacacg aattagaaga tgcagaaaat aactatgatt tagaacgcgc tgctgtattg
     1621 cgtcatggta cgatacctca attagaaaaa gaattgaagg aattagaagc taaagcaaaa
     1681 gacagcgaga ttaagatggt tcaagagtcc gttaccgaaa atgagattgc tcaagtcgtt
     1741 ggcagattaa cgggtatccc cgtaacgaaa cttgtagaag gtgaacgaga aaaactaatt
     1801 aaattgaatg aaacacttca taaacgagtg attggtcaag atgaagcagt tgatgcggtc
     1861 agtgacgcgg taattcgttc acgggctgga ttgcaagacc caaatcgtcc tctaggttct
     1921 ttccttttcc taggaccaac tggtgtcgga aaaaccgaat tagcgaaagc gttagctgag
     1981 aacttgtttg actcagaaga tcatatggta cggatcgata tgagtgaata tatggaaaaa
     2041 catgctgtgt ctcgtctagt cggagctcct ccaggctatg taggttacga agaaggtggt
     2101 caattaacgg aagctgtcag acgaaatcct tatacaatcg tcttgttgga tgaaatcgaa
     2161 aaagcgcatc ccgatgtctt caatatcttg ttacaagtcc tagatgacgg acgcttgact
     2221 gattcaaaag ggcgagtagt agatttcaaa aatacggttc tgatcatgac cagcaatatc
     2281 ggttcacagt tactgctgga aggcgtgact gctgatggaa cgattccaga agcagtggct
     2341 gaacaagtca acacgctatt acgtggaaac ttcaaaccag aattcttgaa ccgaatcgac
     2401 gacacgattt tatttacacc attgagccta gacaacgtga aaggaatcgt tgacaaaatg
     2461 gtggcgcaat tggcacaacg tctggaacat caagaaatcc tgctgacaat cagtgatgaa
     2521 gccaaaacgt ggattgctga aaatgcttat gaaccagcat atggtgcaag accattgaaa
     2581 cgatttatca ctaaggaagt agaaacacca ctagctaaag aaatcgttgc aggacatgtt
     2641 atgcctaaat caaaagtaac gattacttta cttgatggac aacttcattt caaaacagaa
     2701 gagctagaag aaatcgtcta atcttccatt gagactataa aagaaaaaag gggtctgaca
     2761 caactagtgt cagacctcct tttttcaaat aaacagtgtt ccagaagtag tttcttcgaa
     2821 aaataagccg aaatttcttg aagctgcccg cttctgtcac aacctctttt attaggtatt
     2881 tttcactttt tctaatttat aactttcaaa gtaacgctca tcttttttac tttgagacca
     2941 tgcaaacaat cccattaagg cagcacctat cgtatttgtc agcaagtcgc ccatcgtatc
     3001 catcaatgct gctcgtccga caaacagtgt gccatcagaa gcagcaaaac gttgaagatt
     3061 catgcctaag aactggtcgc ataagaattc ccagaactcc caaaacacgc cgcaaagtcc
     3121 agcaaacgca aagccgaata agaggaacaa ccaaggactg gtttttgata tctcagcatc
     3181 tttcatcaag taaccaatca atccatagcc aagagctgtc aggaccattg gactcaccgc
     3241 atgtaagatt ttgtcccaaa aagagatgat actgatcaaa tgcatccctg tgcctaaaaa
     3301 gacagagatg aacaagaaaa accagtaaaa caaaacgatt gctgctggta ttttaagttt
     3361 gaaaactttc aaaatgactt gtggcatgaa aatcaatgca atccctaata aagattccac
     3421 gataaatgtg attccttgag aagtcgaata tttttgtgtg ataaattcat aaaaattata
     3481 gcagacaacg aatacaccaa aagcaagtaa agctatttgc acctttttga tttctttaat
     3541 cactgaaagt ccctccatct aaaaatcttt ttacttctga aagcaactca atttctgaag
     3601 aaaccacttt tccgttcaat ttgatcaaac ctattgtata taagttcagg tagtgaaatt
     3661 gattttcagc tgtttcttgt agtgcagcga tttttttcgg gtgggttccg ccttgctgtc
     3721 gagtatcagt atataagccg atcactggga tgcctttcgc ataagccaca ccgatttccg
     3781 atgctacccc ggcatcgatc gtcagcccat ctagtaaagc aatcattaaa tcactagcca
     3841 acactttttc tgtgtcagca agagcaatca ttcgactgtc tgcataagca gttttgtcat
     3901 tgattgcttc attttcttga ggaagataga tcgaaagctc agaagagagc tctctgattt
     3961 ctttaactaa ctgttggtta tataacaaat cacttttagc aaacattggt gcggcaaaat
     4021 aaattttcat ctaaacatca tcctttttat caaaaataat cgctaaagta agcttccatg
     4081 tttggaatga tacgctctag tgacttcagt gtttcttcat ctgtttctaa ccgttcgatt
     4141 cttgccaaat aagaagcacg gcgataattc atcatcaagc aagtcaatgt ttggatatcc
     4201 agtcgaacgg attttcctat aggttcattt aagacacgga cttgtccctg ttcatcccat
     4261 gttaaagcga agacaccatt attccaagga gcgacaggat cttcaatgat gaaatgaaaa
     4321 gcatcagctg ttccaacaaa cggaaaccgt tgaaggaatt cttttacatc aacgatccga
     4381 gccataaaat atggttcgat ctgctctttg atctgactat cttcaatcaa aaatgctaaa
     4441 ggctcatttt tataaatatc tccatgtacc cagtagatca tggaaaaatg tgcggaaata
     4501 aagttccata agccatttcg agcttcttga tcaagataga acatttcttt tacgtgaaat
     4561 atttcttcag ctacccagta gaacagaaca cctcttggtt catggttgct atcgtagtag
     4621 acagcggctg tgcgctcatc ttcattttcg aaacgccaat actcttccca atgaaaatca
     4681 ctgcggaata aagcaccatg gttttgttgg gcaaatttgt cataaacgga aaatacgtct
     4741 ggatgatcaa ctggtaatcg ctcaaccata ccacttactg ggatttgttt aggtagctgt
     4801 gtatctctga ttttaaagga aagtttatca gacatgattt cccatccttt acgccgatag
     4861 taggggatac tgtaaggata caaataagag atccattgct tattcttgcg catattatcc
     4921 aatgcaacga taattagatc ttgcatcaat ccatggttag catattctgg ataagttcca
     4981 actccagtca caccacccat tttataacgt gcaccgtgaa tattgacttc acaaggatag
     5041 atagcgattt gagagatcag tttattttcg tggaaccatc cgaacacttt cgatacttcc
     5101 aaaatcggtt ttttggaacg gataaaagct cgtttattct caaacccgct ttcctcgatg
     5161 tctgcttcag tcacttggaa cacataggaa agcaattcat tgaattgatc gatatgttct
     5221 tgagtgaccg gttttaattc aagattttct ctaaaatctt tttgatccat tttttcatct
     5281 ccatttatta gcttgataac agtatagcaa aattctacaa aaaaaacttc tatagctgac
     5341 taattaaaaa ctctttaaat tactgctttt cgttggtata atggaaagtg ctgattatat
     5401 gaaaagagga gacgctatgg atataaagaa aatgaaagaa cgccaagagc agattcgtaa
     5461 tttttcgatc atcgctcata tcgaccatgg gaaatctaca ttggccgatc gtattttaga
     5521 aatgacgcac actgtgacgt ctcgcgaaat gcaagatcag ctgttagact cgatggattt
     5581 ggaacgtgaa cgtggaatca cgattaaatt aaatgctgta gaactcaatt acacagcgaa
     5641 aaatggagaa acatatatct ttcacttgat tgacacccca gggcacgtcg acttcaccta
     5701 tgaagtctct cgaagccttg ctgcctgtga gggtgccgtt ttagtggtcg atgcagcaca
     5761 aggaatcgaa gcacaaacat tggccaatgt ttatttagcg ttagacaatg atttggagat
     5821 cctgccagtc atcaataaaa tcgacctacc cgcagcagat cccgaacgtg tccgtcaaga
     5881 aatcgaagac gtaatcggaa tcgatgctag tgaagctgta ttagccagtg ccaaatcagg
     5941 gattgggatt gaagatattc tcgagcagat cgtggaatat gttcctgcac caagtggaga
     6001 cttagatgca ccgctaaaag ctttgatctt tgactctatt tatgatagtt accgcggtgt
     6061 tgttctaaat gtccggatca cggacggtat ggtcaaaccc ggagacaaaa tcaaactgat
     6121 gagtaatggc aaaacatttg atgttacaga ggtcggggtc ttctcgccaa aagcagttgc
     6181 tagagacttt ttgatggtcg gtgatgtcgg ttatatcaca gcaagtatca aaactgtcca
     6241 agatacacga gtaggggata cagtaacgtt agcagacaat cctgccgctg aagctttacc
     6301 aggttaccga aaaatgaatc cgatggttta ttgtggtctt tatccaattg atacttctcg
     6361 ttacaacgat ttgcgggaag ctttagaaaa actgcaattg aatgatgctg ctttacaatt
     6421 cgaaccagaa acctctcaag cattaggttt tgggttccgc tgtggattcc tcggacttct
     6481 tcatatggat gtcgtccaag aacgcttgga acgggaattc aatctagaat tgatcacgac
     6541 tgcaccatcc gttatttacc atgttaataa aacggatggt acgactgcta cagtagataa
     6601 cccggcagat tttcctgaac ctgtaacgat tcaggatgta gaagaaccat tcgtcaaagc
     6661 acagatcatg gtcccaaatg attttgtcgg tgcagtaatg gaactttctc aacgaaaacg
     6721 gggagaattc attacgatgg attatttaga tgattaccga gtaaatgtcg tctataatat
     6781 tccgctttct gaaattgtct ttgacttttt cgacaaattg aaatcaagca cgaaaggcta
     6841 tgcctcattg gattatgaaa tgtctggtta tcaaaaaagc aagttagtga agatggacat
     6901 cttgctgaat ggagaaaaag tcgatgcatt aagctttatc gttcaccgag actttgccta
     6961 tgaacgtgga aaagcaatcg ttgaaaaact gaaaaaacta atcccacgcc aacaatttga
     7021 ggtgccaatc caagcagcta ttggacaaaa aatcgttgcc cgttcagaca tcaaagcctt
     7081 gcgtaaaaac gtcttggcta aatgttatgg tggagatgtt tctcgtaaac gtaaactctt
     7141 agagaaacaa aaagaaggga agaagcggat gaaacaaatc ggctccgtcg aagttccgca
     7201 agaagccttc atggccgttc tgaaaatgga cgaggacgaa ccgaagaaat agcaagggtt
     7261 tgtttgaaaa cagtgtaaaa aataaaccat gtttacatgg atttgccacc ggtttgccac
     7321 cggtg
//
LOCUS       DAZULO010000116         7283 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000116 DAZULO010000000
VERSION     DAZULO010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.119"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..3319)
                     /locus_tag="ACG8OA_002532"
     CDS             complement(170..3319)
                     /locus_tag="ACG8OA_002532"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFS4977273.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3333..4550)
                     /locus_tag="ACG8OA_002533"
     CDS             complement(3333..4550)
                     /locus_tag="ACG8OA_002533"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS4977274.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4540..6135)
                     /locus_tag="ACG8OA_002534"
     CDS             complement(4540..6135)
                     /locus_tag="ACG8OA_002534"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFS4977275.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            6260..7045
                     /locus_tag="ACG8OA_002535"
     CDS             6260..7045
                     /locus_tag="ACG8OA_002535"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HFS4977276.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            7038..7280
                     /locus_tag="ACG8OA_002536"
     CDS             7038..7280
                     /locus_tag="ACG8OA_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977277.1"
                     /translation="MTNNILISIYPRYMEKIINKQKNFEFRNFYLTSDPYNIFWIYET
                     IPTKALKYKLIVKNPITKLKKNQHYFLGDDEFEQLV"
BASE COUNT         2132 a         1477 c         1199 g         2475 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag
      361 aagcatgtaa ttctttctca gaaacaaacc actcattgga aaaggcatga atcaaattat
      421 cacttgcttg tgtaaaatag cgacgtttaa tgacaaagat atcttcatta ctatctaaag
      481 tccccacatc aaatgcattt aaaatttgtc ggtaaacttc tttgactcct tctgttttat
      541 tcaatttagc caaagctttt tcaatttctt ctcgtgcatc tgaactattc gcagcataag
      601 ttcctagtaa ttgatcaata taagtagaat caatatcata caacttagtg gtattttcac
      661 tataaaattc gatcgttgaa aaatcttctc cctctttttc tggttcttcc tccatcaatg
      721 aacccttcac ggtctcgtac acgccaattt gttcttccaa aagttgaatt tgattgttca
      781 aagttgaaga ttgcaccatg tcatcattgt aatcatcata agtcactaac gattcatacg
      841 cacgattcaa ttcttgaaat gctttgacgt actcgatacg gatttcaagt ggcgtatgtt
      901 catcaatgga ttccatggat gaaacctctt ttaacttacc atatgcttgc ttgaatcttt
      961 ttttggattc ttggtaactt ggataaatca aacccgattc ttctttagct tctgaataca
     1021 gttttgtcgc atcctcgaca ttcttctcca ttgtcgctgg ctttcggaaa gtaacaatta
     1081 gtccttttgt tttttcagga tacgtacggt tcgtacgtga aaaggcttgg atcaatcctg
     1141 catactctag attacgatcc acaaataggg tttgaatcgt aggggcgtca aaacctgtca
     1201 atagacgatc cactacgatt actaaatcga tttgtttccc aaattgtttg aattccgctc
     1261 tttttcgtgc caaacgatta ttgatgtctc cgttataacg ctcaatgtct gctagtgacc
     1321 aggcagtatc ataatactga ttatattcct caataatttt ttgcatttct tcttgttggg
     1381 cacttgaatc tcggctattt tcttctaatg aataggtaat agcgattcgt gggaaatcag
     1441 gatcctccat cgttcgtcct tcacggatag gctgattggg aaactctttt attaaccaat
     1501 cagattcttt cgtcatctct ttgatggccc gataatactt tttggccata tcaatggaac
     1561 tcgtggttaa gatcgcagac ttcgttggac ggccatttcg aaaatcaaat tttgtatagg
     1621 cattgtctgg acggaacatt ttatgaatga ctttttggat atgttcctct gcttcaaaaa
     1681 cagtagggtc caaataactt tcttttttga cagactccat ttgatcgatc atacgattga
     1741 tctgttccgg tgaatactct gcatatgttt ctacttcttt taatttccga tagattttat
     1801 tctctaatga agtcggctcc acggtgtttt catgctctac ttgaaatcct aaaaccgagc
     1861 catcttctaa tgcattcttg atggtatagg tgtgcaagac ctctccatac tgatcatggg
     1921 tcgtacgtgc caattgccct ttggcttgtt tccgattctc agggaaaatc ggtgtacctg
     1981 taaagccaaa ccaagtggat ttcggaaaca tttttttgat ttcttccata ttttcagcac
     2041 ttaacgctcg atgacactca tcaactacaa acacgatatg ttgccccatt aatttttgga
     2101 agcgattggt tccttttttc tcttcctgtt tctttgcata tttcaacgcc gcatctagtt
     2161 tttgacgggt tgtaatgatc acaacattcg cattggcatc tgccaacaga gtttcactta
     2221 actctttggc acttcctgtt cccacgatca agctattcgc tttggcatta ccagaagaaa
     2281 ccccagtatt aaactccgaa gcaaatttcg taaactctgt ggtggtctga ttatctaaat
     2341 ctttgcggtc cactaacatg atcgttcgat cgatccccga ttttcgtgct aaaagcttag
     2401 tagatacaaa actagtcaac gtctttcctg aaccagtcgc atgccacaca taccctgatt
     2461 gatgtttatt tgcggcaata aacaaggctt gaatcgcatg cacttgataa ggatgtaaca
     2521 ccatgagggt tttattgtct tgatcttcac tgacaatcgt ataatccgca atcaagcgat
     2581 gggcatctgg gatatttagc acttgtttgc aaaactcata taagttttct acctttttat
     2641 tatctttcgt tcgccaacta aataaaaact taggatgcaa atccttcggt aaggcatttg
     2701 caaaataccg agtcgtttgt tcattggaaa ccacaaacaa ctgtaaggtc gaaaagatat
     2761 tgttccgaaa catgccttct tcagcatatt ttttgatttg attaaacgcc tgataaaaac
     2821 catcttttgc agttacttgt tttagctcga tttggataat cggtaagcca ttgatcaata
     2881 aggtgacgtc aaaacgtcga tcccgcgctt ctatgttact tccacgtttt gcaatttgat
     2941 ggaccacttc ataagtagag attcctccac caatgtcttg attggaataa aggataagtg
     3001 agacagaacc caattgcgga ttttcccgtt caatcgtgat ccgagccatc ccattttcac
     3061 cttttaacca tttcgcagca tcaaatgggg ttttcgtccg tagcaataat tcggttttga
     3121 tcgtttcaaa ctctttatcg gtcaaaggag aatcattcag ttctgcttga ttatttgaga
     3181 tgattttttg acgtaaattg ctccaaagat cttcttctga ttttagatcc ggacgatacg
     3241 tccactgatt atgtccttcc ccgaggacac gaatcaactg ttcttcgact tccgcttcct
     3301 cacggtgact aattttcatt cttcttccct ccctaaacaa acatcttttg caagaagcct
     3361 tttttcgttt ccttcagcaa atctaactta cgttgatgaa gagcgatcgt atcatctagc
     3421 tgcttgaaga aggcaccaat tttttgttgt tcatttatat ccggtggaaa ctgaaatgtc
     3481 ctttgttcta taccaccttt agtcacatgt accattgtcg ttccgttagt tgattcttta
     3541 attttctctt tatcagtcaa tagccaagta tatagatact gtttgtccaa ttccttagat
     3601 aaaaatttaa gtttccaaat atgataatgg tagattacac gttcttcatt ccacatttca
     3661 ggaccgaagc ttgttgccca taaatataag agatctcctt tattagcata cttgtcttcc
     3721 tctaattcta agtttgaata ataccatcta tcattagtat taaaattacc tactcttaaa
     3781 accctatatt ttccagaatc aagtaattct tgttgtttaa aagctctacc attcaaaaaa
     3841 actaccactt cccccaactt ccgctcttcc caatctcccg taaatcctgg aaaacgaact
     3901 tctggcactt ttgctccgtt tttagggaac attttttgta agaatccttt tttcgtttcc
     3961 ttcagcaaat ctaattcgtg ctggtgaagc atgatggtgt gatcaagttg tttgaaaaat
     4021 gaaccgatct tgatctgttc gtcataagtt ggaagtgaga taggtgtctt tttaccacta
     4081 tcaattgtat aggttccgac ggttccaatg cctgcaacgt cacggatata cttctgaatt
     4141 ttgttattta tgaacgagta gtaaaagaag tttccgtcaa ttttatgctc attcttgaac
     4201 caaatgatgt ttccatcctt aaagtaaatt gggttatcat cagtgattaa taaggggaca
     4261 cctattgatc cgacaccagt tacaagcaag tctccttgag aaactttacc tgaatttttc
     4321 gaatattcat tatatttttc ttctgaaatg taaagataat cggatggttc ctcatttttt
     4381 gcagctgaga caatatcacg agcacgtaaa aatctaatac ctgagttagt ccaatctgat
     4441 tgatgaattc gcttcacaga agtaacatcc atcatatctc ctaacttacg ctcttcccaa
     4501 tcttccgtaa aaccaggaaa ccggatttct ggtcgtgtat cattgctcat gtttgaacac
     4561 ctccagcgca ccttgtaacc acgcttcatt ttcaggtgtt gtttgcaggg aagagatagc
     4621 ttctaaaagt tctttttcta gcacttgctg ttcttgtcgt atcttcttga tgtcatttcc
     4681 gacatgaacc atatcaattg gttcttcttc ttcgaaagta tcgacatagc gtgggatatt
     4741 taagttatag tcattttctt tgatttcatc aaaggttgcc aaatgagcgt atttctcgac
     4801 atcttttctt tcagcgtagg tttcgaggat tttttgaatg ttttcttccg ataatttatt
     4861 ctgatttttc cctttaacaa attcacgact agcatcaatg aataatacat ctcgagtttg
     4921 acgattcttc ttcaatacaa tcaccgtcgt tggaatcgat gtaccaaaga acaagttcgc
     4981 aggcataccg atcacagcat aaatactgcc gtcttctaat aatttttgac gaatgacccc
     5041 ttctgcagca ccacggaata aaaccccgtg tggcaagacg atagccatag tccctgtttc
     5101 cttcaaatga tagaaaccat gaagaagaaa cgcaaaatct gctttggatt ttggcgctaa
     5161 tttcccgtag cgattaaacc gagaatcatc taagaaggtc gtatctgctg accaattagc
     5221 cgaataaggt ggattcatca ctactgcatc aaatgtgtaa ggttcatctg ttggccagtc
     5281 cttattcaac gtatccccat tgcggagatt catttcttcg gcatctaccc catgtaaaat
     5341 caagttcatt tttgctagat tgtatgtcgt tgtatttagt tcttgcccat gatatttaac
     5401 gttatctgga tgagttaagt agttacgtac atttaacatc aaggaacctg aacccatggt
     5461 tgggtcaaag acactaaaga atcgtcgttc tttttgatcc aacgtcacga tttgtgccat
     5521 catatcagaa accatatgag gcgtgtaaaa ttctccggct ttcttccctg cttctgaagc
     5581 aaattgactg atcagaaatt catacgcatc cccgatcaca tccccatcgt gacctaaaac
     5641 ttccacgtca ttcaatttct tgataacttc ggtaatcgtc acatttcgtt gttgctcatc
     5701 tgttcctagt ttttttgatt ggagatccac atcatcaaat aacccattaa attgattgta
     5761 ggtagatgcc aattgcacaa aggctttgtt tagatcgttc aattggaacg ttgcttgttt
     5821 tgcttgatcc gctaagacgt tgaaaagata ctctggtgag atcgaatacc ccaaaatatc
     5881 aacgattgtg gcaatcaggt cttcttttga ttcttcatct gaaagtaatt cacgatacaa
     5941 cattgtttgt ttgcttactg tgtcatattc ctctaaggat tcatctgcta aaagaaccac
     6001 ttgttccaac aaacggtcgg ataaatattt atagaaaatc agtcccaata aataattttt
     6061 atattctgac gcatccattt tactacggag attatctgcc gcactaaata atttttgatt
     6121 taattctgca cccaaaaaga tacactccta tctcttattt aaagttgcta tataccctct
     6181 tattttagca tactcccttt tagaaaaagc aaaaaaaaaa gaaaaaatct tattcttgat
     6241 aacaagctac gtgacgtata tgcctaacaa aaatagaact tttgaatcaa tcttaaatga
     6301 aggtttaacc acagaaccgg catccattat ttttgatgaa gttatcgatg acgttgaaga
     6361 tagtaagtat cataaaaatt taaaagaact tttttatttt gcactcaaca atggaccaag
     6421 tttgttcact ggtatatcag tacctgatag tgataatcca ttaattatgg caaaaattta
     6481 cttaacaaaa tggtgtaata aatacatcag ggataggaat aacccagcat taaaaaaacc
     6541 tcttaaaaca tttggcgaaa aagatgctgc tttaactact agagtagcag caaatgctaa
     6601 tattgatgat caaaaagtat tgaatgacta cttgcgtgga cattttttat atatgagcgc
     6661 tgagaacatg aacggaagta ttttagagga atatttagcc gaagtgttag aacctgaagg
     6721 ttggatttgg tgcgccggct ctgtatatcg agctgttgat ttctgttatt taggaacctc
     6781 accaattctt ttacaagtga aaaataaata caatacagaa agcagttcga gttcagctat
     6841 aagagtagga acaacaatta gaaaatggaa tcgtttaaat aagtcgacaa aaatttctgg
     6901 tttggatagc cctatcccca attggaaagc attaatagaa atgactgaag ctagtaagga
     6961 actggcagct aagttaactg aaaatagtta tttagcttat attaatgaaa aatccacacg
     7021 agaattatgg acactcgatg actaacaata tccttattag tatctatcca cgatacatgg
     7081 aaaaaattat aaacaaacaa aaaaactttg aatttagaaa tttctatctt acctcagatc
     7141 cttataatat cttttggatt tacgaaacaa tccccaccaa agctttaaag tataaattga
     7201 ttgtaaaaaa tccaattact aaacttaaga aaaatcaaca ctacttttta ggagacgatg
     7261 agtttgaaca attagtttaa aag
//
LOCUS       DAZULO010000117         7258 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000117 DAZULO010000000
VERSION     DAZULO010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.120"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..341
                     /locus_tag="ACG8OA_002537"
     CDS             <1..341
                     /locus_tag="ACG8OA_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4977278.1"
                     /translation="VDELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLKTISSTL
                     PQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARI
                     YLQQGLIFEN"
     gene            complement(485..2014)
                     /gene="ahpF"
                     /locus_tag="ACG8OA_002538"
     CDS             complement(485..2014)
                     /gene="ahpF"
                     /locus_tag="ACG8OA_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFS4977279.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(2024..2587)
                     /gene="ahpC"
                     /locus_tag="ACG8OA_002539"
     CDS             complement(2024..2587)
                     /gene="ahpC"
                     /locus_tag="ACG8OA_002539"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFS4977280.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2731..3393)
                     /locus_tag="ACG8OA_002540"
     CDS             complement(2731..3393)
                     /locus_tag="ACG8OA_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4977281.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3577..5223
                     /locus_tag="ACG8OA_002541"
     CDS             3577..5223
                     /locus_tag="ACG8OA_002541"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS4977282.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            5214..6296
                     /gene="uxuA"
                     /locus_tag="ACG8OA_002542"
     CDS             5214..6296
                     /gene="uxuA"
                     /locus_tag="ACG8OA_002542"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS4977283.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(6404..>7258)
                     /locus_tag="ACG8OA_002543"
     CDS             complement(6404..>7258)
                     /locus_tag="ACG8OA_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFS4977284.1"
                     /translation="VKDVQKEFTEYEYIDFFIDLSVDILRPNLPICREQRILVNILAR
                     LSITKIESEETSLEQYKLLKKANVPHVLYSREMVESLCTVFDLFIPEKEKILYSLAFA
                     RNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILEK
                     ENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQNDF
                     MENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQS
                     KKENQVTS"
BASE COUNT         2251 a         1361 c         1362 g         2284 t
ORIGIN      
        1 tcgttgatga gctgttaagc tacaacgaag aattgcgaac tgcgtatcat ttttgtcaat
       61 tattacgcta ttatttcgtt aaacgaggta cggaaggctt tcaagaaacc cttaaaacaa
      121 tttcatctac gttacctcag tcgtttaaaa agaaattcac gatcttccat cgctaccaat
      181 cgggtatttc aaatgccttt aaggtgtccc actcaaacgg cgtcactgag gggctgaata
      241 acaagatcaa gctgattaaa cgaattgcgt ttggttatcg caacttttat aactttcggg
      301 cacggatcta tttacaacaa ggtctgatct ttgaaaacta aaaaaatatg cggcgcacag
      361 aacgcgccgc atatcggact aagttttatt caaatttgtc ttcaccaaca cgatttgacg
      421 aagagcccaa aattcttgtc accatccttt tttccgctct ctgacttttt acaatcttca
      481 tagctcaagt gcgcatcaga taatcgaatg ctcctaaagc tgcagtagct cctgaaccca
      541 ttgaaacaat gatttgtttg tatggactat ctgtacaatc accagctgcg aaaacgccgg
      601 ggatattcgt tgcgccattt ttctcggtca ctatttcatt tcttggattc atttcgatcg
      661 ttccatctag ccaatccgta ttagggagta agccgactag aatgaatact cctgaaacag
      721 ctagtgtatg catctccatc gtacggcgat cgatatagga gagagattct acctgattcg
      781 taccgctgat ttctttcgtc gctgcatgtt tgataacagt gacattcgca agagagacta
      841 gtttatcctg taagatttga tctgctttta attctggtaa gaattctaga acatatacat
      901 gttttgctaa cccagccaaa tcgattgctg cttcaacacc agaattacca ccaccgataa
      961 cagcaatctc ttttccttta aataaagggc cgtcacagtg tgggcaataa gcaattcctt
     1021 tatttttaaa ctctttttcg cctggtacat tgatagaacg ccagtgcgca cctgtcgcga
     1081 tgatggcggt ttttgcttta agtgatatac cgttagctaa gccaatatta ataaaatgtt
     1141 gctctttatt taaggaaacg gcctgttgat tcatcataat gtcgataggg taactacgca
     1201 catgactttc tacttgctgc atcaattttg gaccttctgt gtaagaggta ccaatcatat
     1261 tttcaatccc tagtgtttca acgacttgtc cgccgaaaga atcagcgaca agtcctgtac
     1321 ggattccttt acgtgctgca tagatagctg cactactagc tgctggtcca ccgccaatca
     1381 ctaatacatc ataaggttct ttttcaaaca actcttcttc aatcaaaggg ccagtgattt
     1441 tttcaacaat ttgttcgatc gtcatgcgtc cgctactaaa ttcttctcca tttaaaaaca
     1501 cagttggcac tgccatgatt tttttatcat cgatttcttt ttggaacatc ccaccttcaa
     1561 tcatcgtatg agtaatattt ggatttaata cagctaatat attcaatgcc tgtacaacgt
     1621 cggggcaatt atgacaagtc aagctaacat aactgtcaaa atgcaatgtt tgtgttattt
     1681 ttttgatttt aagactcaaa gattcactga ttttcggtgc ccgtccacta atttgtaaca
     1741 tagccagtaa aaaagaggaa aattcatgac cgagggggat accagcaaaa acgattccac
     1801 tttctttgcc aactggattg atagaaaaac taggtgtgcg catcgaatct cttttttccc
     1861 aagagatttt aggagacatt tctgctattt ccttcgtaaa ttcagctagt ttttgactat
     1921 tttcactatt atccaaacta acagaaaaaa caataggaga ttcaatcagt gctaaatatt
     1981 gttcgagatt ttgtttagtt tctttgtcta acatatccac cacctaaatc tttccaacaa
     2041 gatcaaaact tggtttcaat gtttcgccac tttctttcca ttttgccgga caaacttcac
     2101 ctgggtgggt acgtacatat tgagcagcgc gaatcttatc aatcagtgca ctagcgtcac
     2161 gaccgattcc atctgcattg atttccatag cttgaacgat tccgtctgga tcaataataa
     2221 atgttcctcg ctgagctaat ccttgctctt catccaaaac atcaaaaata cgagaaatct
     2281 gatgagacgg atcaccgatc atcacgtatt cgattttacc gatagcatct gatgtatcat
     2341 gccatgcttt gtgagtaaag tgggtatcgg tagagcaaga aaagacttca acacctaaag
     2401 atttcaatgt actgtattgt tcttgtaagt cttctaattc agtaggacaa acaaaagtaa
     2461 aatcagctgg atagaagcaa agaatactcc agttccccat aagatctttt tcactgactt
     2521 ctaaaaattc tccttgatga taagcatgtg cattaaattc ttcgattttt ttcccaatca
     2581 atgacatagt aaaacctcca ataataatta tttatttata attattgtaa tttagttgga
     2641 gttgcaaagt caatggatat tttgttattt aataaagatt actcaaaaac gaaggttaat
     2701 tataaaaata gaagaggtcg caagaaaatg ctactgctgg aaatactcgg gataacgttg
     2761 agtaacagtt tcctgttctt tcagcatcat gtgtaagtgc aatcccgttt gaaatcgagc
     2821 ttcttctggt tttttatcaa ctaaagcctt gagtaaatca taatgttgtt ccatgatcgt
     2881 ttcccatgga agcccctgaa tggtcactcg taaccagcgg aaacgttcta aatgggtatt
     2941 atggtcatct aaccagttcc aaacttcttt tcttcctgca atttcgaaac aagcttcatg
     3001 aaataaatta tctgcacgga aaaaagcgcg ttcgtcattt attttgactg ctttttcttg
     3061 ttcttctaaa atcgtttcta gcactattcg tgtttgtgtg tccaatttgg aacaacattc
     3121 aatcatgatt tgctcttcca actgttctcg gacaaagcga gcattttctg cagaaacaag
     3181 attaattttg gaaacatacg taccactttg aggtatcgta tacacgagtt cttgctgacg
     3241 tagttgaatc aatgcttcgc gaatgggagt ccggccgata gcaagatgtt cctccagtgc
     3301 cttttcagat attttccttc caggttctaa gtcagcatag ataatttgtt tacgaatcgc
     3361 ttgataagct tgtgattgca agctgctttt catctttatt cctccaaaat catttctatg
     3421 aacgtagtat aacagaagag gtctgaaaaa caatctggta tgtcacatta actagtaaat
     3481 ttgtataaat agtaaaattt tggttgacat agcgattttt attgcataaa attcacttga
     3541 atacaaaaac taatatgtta gtttaggagg ggaaaaatga ttgatttaaa caactggagg
     3601 aactttatcc ctgaattatc tcaaaaagaa gtagaaatac ctcattttga ccgtgcatca
     3661 ttaagagaaa atggacaaaa aaatcctgtc tggatccatt tcggtggcgg aaatctttat
     3721 cgaggatttc atgcagaaat cgctcaacgt ttagcggatc taggcgaatt gtccactggt
     3781 gtcattgttt gtgaaacatt cgatgaagaa ataattgaaa gggcttacca agattttgat
     3841 gatttgattc ttgaagtggt catgccaagc agtggagagt tcgtcaaacg tatattagct
     3901 gcgacagctg acagtctgta ttgtcatcca gattgttctg aacactttga taagatgatt
     3961 cgacactttg aaaatcctag tttacaatta gttacttttg ctattactga aaaaggttat
     4021 cgattgaaag atacaaataa taaatatata ggaccgattg cagtcgatat aaaaaatggt
     4081 ccaaagaagc catcacatac aatgagtatt cttgctgtgc tactttataa acgtttttta
     4141 tctggagctt ggccgattgc acttgtctct acagataatt tttcacaaaa cggtagtcaa
     4201 ttacgtactt ctttgctaac gatcgcaaaa gaatggcaaa aggaaggtta tgtacaaaat
     4261 acatttatcc aatacttatc gaatgaagaa attgttgcgt ttccatggac gatgattgat
     4321 cgtatcaccc cgaatccttc cttgtcagtt agcaaaatgt tggcaaaaga gggcttttca
     4381 aatatgtcga ttttccaaac cgcaaaagga aataatattg ctccatttgt caatacagag
     4441 gacatccatt atttaatcat ggaagatcat tttcccaatg gacgtccgaa attcgaaaaa
     4501 gctggggtat tcttgactga tcgtgcaacg gtagataaag cagatacgat gaaggtgacg
     4561 acttgcctta atcctttgca tacgtcttta gctattatgg gttgtttact ggggtatagg
     4621 agcatagctg aggagatgaa agatccagat ttatcgctgc tagtttctga aatcgggtac
     4681 aatgaaggcc tcccagttgt ggagtctcca ggtatcattg atccaaagat ctttctggaa
     4741 gaagtaatta ctgtgcgctt ttctaatcca aatattccta atatgcctca gcgaatcgcc
     4801 acggatacct ctcaaaaggt ggcgattcgt tatggtgaga cgattaaaaa atatatggaa
     4861 atagaagtta aagacccgca gacacttgta ttcataccat tgactctcgc tgcttggctt
     4921 cgatacctta ttggcattga tgatcaagga aaaccattca tcccgagtcc agacccacta
     4981 ttgacagacc ttcagcataa gctttccgat attcaactgg gtacgcaaga tacatcacat
     5041 attcatgaga aaatggctcc tattttgtca aataaagaaa tttttggtgt agatctttac
     5101 caagcagagc ttggcagaaa aatagaagaa atgttcatca agatggtagc aggaccaaat
     5161 gccgtacgaa aaacattaca ggattactta caagaacatg gaggaaattt atcatggaaa
     5221 tgacttttag atggtacgga tcacaggaag aaaagattac cttagatgaa atcagacaga
     5281 ttcctgcggt caaagggatt gtaggcacat tgatggatat accagtagga gaggtatggc
     5341 ctagggagag gatcaaagaa ctaaaagaag aaattgagtc tcgtgggtta acactaaaag
     5401 taattgaaag cgtcaatgtg catgatgcca ttaaaattgg attaccttcc cgtgagcagt
     5461 atatcgaaaa ctataagcag acaatccgaa acttggcaga atatggaata gaagtcatct
     5521 gctataattt tatgcctgta tttgactggg tgaaatctga ccttgactac cgcttagaag
     5581 acggctcatc cacgttagct tttatatctg atgatattcc agatgatcca agagaaatcg
     5641 tcgcgcgcat cgaacattcc agcaatgaat ttggattgcc gggatgggaa cctgagcgct
     5701 tagctcctat caaatccttg cttgaagcct atgcatcagt agacgaagaa aaacttcgag
     5761 aaaattttgc ctattttctc caatcaatca tcccaacatg tgaagaggta ggggtcaaaa
     5821 tggctgtaca tccagatgat ccgccatact cgatttttgg attagctcga atcgtaaaga
     5881 atcgagaaga tttagattgg ttgtgtcgca tagtagacag cccttataac ggaatcactc
     5941 tttgtacagg gagtattgca gaagatccag aaaatgatgt atatgggatt ttagcagaat
     6001 tttgccaaag agatcgaatc ccatttgcac atgttcgaaa tattaaattt ttagctaatc
     6061 gagatttcta tgaggcacca cataaatccg aatacggttc attagacatg taccgcatac
     6121 taaaaacaat gtatgacaac ggatttaacg gatacatccg tcctgatcat ggaagaatga
     6181 tctggaatga gacaggaaga ccaggatacg gcttatatga tcgagcatta ggtgctgttt
     6241 atctaaatgg attatgggaa gcaatcgaaa aaggaaacaa aagtaaagat aattagtgaa
     6301 aaaaactgta attttattat gtaaataaga aggatgttca aattcttgat acataaatcc
     6361 ggaggttgtt aataagcatt cggatttatg tatcttattt ggtctaggaa gtaacttgat
     6421 tttctttttt cgattgatcc atgagtgctt cttcttttaa ttcaaagata tattctgaga
     6481 tcctcataat cacttttttc cagtcttctg gtgtgatgat tccttttaaa atatagattt
     6541 ttttgatatg agaaggcaat tgtttcaaag ggcagtcgct gataacgatg ttgcagttct
     6601 ccataaaatc attttgtatt ttgatcgatt catttgaaaa aatttgttct atcttaacga
     6661 tcagatcttt actgatataa aaatcacgcg tatagttcac accaatcaca attcgcggct
     6721 gttttttgta gcgatcatac aaatgtacta ggtcttcaac catccattgc acattttcct
     6781 tttctaaaat aactggtaac aagtgttgat tttgttctct gaaatccata taaaattgtt
     6841 caatggcggt ttggacttct ttcaatacgc tatgttcttg ataaagcatg aaagtaggaa
     6901 ggggcgtatt tggttgattg aagttaagaa atccattata aagtttattt cgtgcgaatg
     6961 ccaatgagta aagaatcttt tctttttcag gtataaacaa atcaaataca gtacaaaggg
     7021 actccaccat ttctcttgaa taaagaacat gaggaacgtt tgcttttttt aaaagtttgt
     7081 attgctccaa gcttgtttct tccgattcga ttttcgtgat agaaagtcga gctaaaatat
     7141 tcactaagat gcgttgttcg cgacaaattg gcagattagg tcgcaaaata tctacagaga
     7201 gatcaataaa aaagtcaata tattcatact ctgtgaattc tttttgtacg tctttcac
//
LOCUS       DAZULO010000118         6693 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000118 DAZULO010000000
VERSION     DAZULO010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.121"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            209..2884
                     /locus_tag="ACG8OA_002544"
     CDS             209..2884
                     /locus_tag="ACG8OA_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFS4977285.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3066..3305
                     /locus_tag="ACG8OA_002545"
     CDS             3066..3305
                     /locus_tag="ACG8OA_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977286.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3321..4694
                     /locus_tag="ACG8OA_002546"
     CDS             3321..4694
                     /locus_tag="ACG8OA_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4977287.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4698..6332
                     /locus_tag="ACG8OA_002547"
     CDS             4698..6332
                     /locus_tag="ACG8OA_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4977288.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6495..>6693
                     /locus_tag="ACG8OA_002548"
     CDS             6495..>6693
                     /locus_tag="ACG8OA_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977289.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2352 a         1075 c         1338 g         1928 t
ORIGIN      
        1 taattgaaca atattttcct aaataattta ataaggattg atttatcgta aaagaaaaaa
       61 gttgtgattt ccaaaatcac aacttttttc ttttgtcaag attttgagtg ggatatattt
      121 ttgtagaact gaagatcatg aaattatgag aggttggagg taatgtagtc atacaaataa
      181 aaatttttta gaaagtgagt ggtaattaat gaaaatgaat cacacttcaa ttcagaagca
      241 tttacaactg ttaaatacac tggggatttc ttatgttaat ttgagaactt tttctacatt
      301 aaacaagaaa accgtttctc atattttttc tactgcagat tctcaagcaa ttattaacta
      361 tttagaaaaa cagaaattgt ctgaaggtac agtaaacata acttataatc ctattaaaca
      421 agaggtgatt acacaaaaaa agcattctgt gagagacaat gatattgaaa aaattcggtt
      481 tgtatttatt gatattgacc ctaaaaggaa agagggaagt gcaacagata gcgaaaagaa
      541 aaaggcagaa aatgttatgg aacaagtaga acgttatttg aaagaaaaga gaatagaatc
      601 atttgtaaaa gttgatagtg ggaatggcta ccatattttt cttcccataa atgaacaacc
      661 aaataatcac gagacgattc ttactattca gaattttctt caattacttc accgaagatt
      721 tggagtagaa gatgaagttg atattgatac ttctgtttat aatccttcaa gattatgcaa
      781 attagttggt acaccagcgg tcaaaggaaa gtccacaaaa gagcgtccgc atcgtttatc
      841 tagtttcctt tctattcccg ataacataga tagaagtaat tgttttaaaa ttcttgaaca
      901 gatagttgaa gaaaattcca tgatgtcaac ctctagagta aaaagccatc catcttttag
      961 agaagtgtta ccttttattc aagcagacta taaacaatgg ttagctcact atcctgaatt
     1021 aacttatacc ataaaagagg gcgatcataa aggagttacc ttacttatat ttgacgaatg
     1081 tccattaaga aaacacacga ataattctaa tggttcttgt ttgtctgtta cggctgataa
     1141 taagattcga tttcattgtc tgcatgcttc ggataaaaat aaagatattc aagactttgt
     1201 aaaaaaatat ccgttgccaa aacctgtaaa tccttctgta gaatcattag aatccaatca
     1261 agaatattat tttgaccagt ttaaactgaa tcaagaagga gtatttttgg tgaaagaagg
     1321 tcaagaagaa aaagttgctt gtccgatgtt tattaagcaa atacgcaaaa atatcgaaca
     1381 aaaagtcatt acacttgttc ttgcctatta tgtcgatcat tgttgggatg aaatagaaat
     1441 agaaggtgga tgtttacaaa ctaataattt taaacaatta gcaaaacatg gcttaatcat
     1501 taaagctaga agagaagctg aagtcgttga ctttttacag attcaacggg agaatgctcc
     1561 aatctttttt gaacatgaat ctcttggctg gaaaaaagaa aatgaagcaa tgatttttca
     1621 gttacaagat tcttttggac gtgaaattcc ttctgtatta actaaaaact catgctacca
     1681 attaacaact aatggtacag aagcggaatt tgacgcattg gttcataagt atgttttagg
     1741 aacgaactta gaacttgctt ggtcgattgg tattgcttct attgtgttag gctatttaaa
     1801 cgttacgaag gctaatactt ttttgagttt actaattaac ctacagggca agagcacaac
     1861 gggtaaaacc actacgttac actttattgc ctctttattt ggaaatccaa ctacggttct
     1921 tagaaatatg aatgctacca ataaagccat tataaagcta gcatcaaaca atcagggagg
     1981 tgttccacta atactagatg agttaggagc agcaactcaa aataacttta gctcgttgat
     2041 ctatcaatta gcttcaggag aagaaaggct aagattagat tctaattgtc agcttatgcc
     2101 acaagagact ttttctgttt tgacccttac ctcttctgaa gagcgtttgg aaaattattt
     2161 caatgagaaa ttaactggtt tgaaagttcg atatatcgaa ttttctgata tcaagtttac
     2221 aaaggatgcg aatcacgcag agaaagttaa aagtctttct tctaatactt ttggttgggg
     2281 tataaagtta tttcttttga aactctttga acaagggatt acaaaaattg aagcaacttt
     2341 tgtcgaagct caaaaaaaac tatgggctgt tttacctgaa cattctttga agcaacgcat
     2401 tggtaataat ctagctttga tttatagtgg agccctccta attcaagata tactgggcta
     2461 tcctttacga ttagagcata tacagacggt gcttattaat tcgtacgaac agttaattgt
     2521 agatactagt ttaggaagtg agcaaacggt atttgaccga gtgaaaacat tgttggtgat
     2581 gaatagccat aaatttttaa caaacagtga caacaaacat acgcccagta cgatttgggg
     2641 aaaagtatca attcaaaaaa atggggatat taaggtaaat atttttccta tcccgtttga
     2701 ggacttgtta aggcgagaat ttcaagtaaa ggatctaacc tatcttttta ggcaattgct
     2761 tgattcgggt aatatgtgtg cagaaaaagg gagaagaacg aaacgtattc acatgaatcg
     2821 tcaagttctt ccaacatacg agattctatt accttcttca ctcaaatcgt attttcgatt
     2881 gtaatttaga ataaaccaaa gtatgaaagt gaggcaccat gttatggaaa tatttcttat
     2941 tgatttagca aaacaactga ttacttatct gtatgatagc gagcaaaccc aaacggaagt
     3001 aaatgaagct tgatctatgc ttcatttact tcattcaatc aaacatgaaa gaaaggaaaa
     3061 ttctaatgac taaaaataaa atgacactaa aagcagaggt tcttctgtat attcaagaac
     3121 atttttcaaa tcaagccttt tttacaaaac caatttactt ggatttcgaa ataagagggg
     3181 tatctgctgg atctatcggg ggtactcttc aagcgttgaa gaacgaagga tatttagaaa
     3241 atcactttgt gcaacgttcg ttcaatagaa gagtcgtgaa aaaatggtat ttagttcatt
     3301 catagagagg ggaatcaagc atgcgtgtgg cttgttatga tcgaacgtct gtttatgaag
     3361 aagcgaaaat tagtaatgac gctcaatatg atgcagtgaa agagtatgtt ttagagaaat
     3421 atgaacaaat tccccaacga ttttcagaca aaggagaatc aggggaaaaa gcggagcgtc
     3481 cagcttttcg acagcttaga gcttgggttt gtgcgtcaga agaagagggg gtgatctatg
     3541 tttggcgtta tgatcgattg gcacgtcaag ccaaaatagc cttagaattt attgagttgt
     3601 gccaacaaca tcaggttcaa gtgtattcga tttctgaacc tcttcctgct tctttctcac
     3661 acgcgtcaac gaataaacta tttgtgcaat tattgttcac ttttgcagaa caacaacgaa
     3721 acgtcattat tgagaatgtt cgtgccggac tttcctataa aaagcaacaa cggcaatatt
     3781 tgtcttctgc ggtaccatat gggtatcgtt tagtgaatgg gaaaatccaa caagaagtac
     3841 acgaagcaag gattgttcgc tatatttttc aactttattt gaccaaaaaa tatggctaca
     3901 aaaagctttg tcaacgattg acgcaacaaa agcttttctt ccgcgaacgt ccttttcagc
     3961 cctatcatat ttatagtatc ctaaaaaatc cattgtacta tggcgaaatc aaaggaggat
     4021 ctttggggaa gtatctagga acctttgaac cgattctctc gaaagcaacc tttttacaag
     4081 cccaagagat tcgccaatca aggtgtacag ctaaaaaaga tacgtatctg tacttattac
     4141 gtcaaaaaat tcggtgtccc ttctgtggtc gtcatctgag ttcgaagtat caatggaata
     4201 caaagaagac gaagacgctt cattattatc attgtaccga ccgaagctgc caaggaattt
     4261 ttcttcgggc agaaagggtt gagtcagaag tattggaaaa gatacaggct ttttttcaat
     4321 acgaaggcgt ctatcaagcc ttgattgccc aattaacgca acaattagcc gaaaagcgaa
     4381 aagatgaaaa acgcaaagta atccaacaaa agaaacaaga agcacaatgg attaaggcat
     4441 ttgaagaggg caattgttca ctggaagaac tcaagcaaca actgcaagct cttcaaaagc
     4501 cgatgtctac ttcttcgttt ctttcactcc aacagtgtga agcaaaagtg aaagaatggc
     4561 ttcaactaaa agacctctct agtcgacaat tattatgtga ctatgtttgt cggattgagg
     4621 taacaaagga taaaacagtt tgtgggattt acttccgaca actgacacaa aatattttag
     4681 aaaggaacga atgatctatg gaaatgatac aaaccaaacg agtcgcattg tatattcgcg
     4741 tgagtacaca agaacaagct ttagaaggac atagtattca agcacaagaa caaaatggac
     4801 gacagtttgc cgaacggatg ggctatgaag tggtagaagt ttatgccgat gaaggaatca
     4861 gtgggaaatc cacaaaaaat cggttcgcct atcaacgaat gatgaacgat gctcggaacc
     4921 atcgttttga tctactagtg atttggaaat tgacccgtct cggacgcaat atgttagatg
     4981 tcttacaaac agtcgaggaa ttattgcgat atgacgtagg attacattcg atctcggaac
     5041 aatttgacgt gaccacttcg acaggtaaat tgatgttaca actccttggg agtttcggtg
     5101 aatttgaacg caaccaaatt gcggagaatg ttcaaatgtc aatgcaaagc ttagtgaaag
     5161 ataaaaagcg ttatgcagga ggccgacgtt taggctatgt cagtggtatt gacacgaatg
     5221 gtcaaaaaca attggtcatt gaaccagaag aggcgaaaat tgttcgtctg atctatgcaa
     5281 aatatttagg cggagcaggg tatcgagcga ttgccaattt tttgaatcgt caaggatatc
     5341 aaacagtcaa aaagaatgct ttttctacca cggcagtcaa agatatctta cgcaataaaa
     5401 tttatgcagg gtatttagaa tatgcgcgct acatcaattg ggaaaccaaa cgacggaagg
     5461 ggaagaatcc gagaccgatt ttagtgaaag gaacccatga accgattatt tcggaagaag
     5521 attatcaaaa agtacaagaa cgccttgctt tagaaggaaa acaacccaaa tggaaccata
     5581 caggtgaaaa tgtattgaca ggcttgttac gttgtccaga atgtggcgca ccgatggcag
     5641 cgagtaatgt cacaaatacc ctaaaggatg gcacaaagaa acggattcga tattattctt
     5701 gttcagtctt tcggaataaa ggagcaagtg tgtgtcatgc caattcgatt cgtgccgaac
     5761 aagcggaaac atttgtcgcc gaccgattaa aggagatcgt tcagctacca gaagttcttc
     5821 cccgattggt ggcagcgtta aatgaagaaa tagtgcgaca aagccaacca ttggaacaag
     5881 aactcgtggt gttactggaa cgaaaggaag aattgaagac taaaattgaa aaatgggaag
     5941 ctgctttgga agatagtcct gaattgtttc cgatgttaaa agaccgctta gacgaattga
     6001 ctgaaaaacg acgacagttg cacattcgag aaaatgaaat cttagggatc tttcaacaag
     6061 gagagccgat tcaagtcaaa gatgtccaac ggatattgac taatttggat cgttttcttg
     6121 cccagagtga gaagaagcaa atcaaagcct tgtatcgtac ctttatagag aaaataacct
     6181 ttgatcctaa ccataaggag atgttggaaa ttacgatgaa attcacgcat gccgtggtac
     6241 aacaattgaa tgagcaatat caaatagccg tatccaaaac aaaggatacg gctatttttg
     6301 tgttaaaaac accctttacg ttaacgatct agtctaggtt atatcaaaca acgttagagg
     6361 ggcgctaacg cttctctagc gatttgaaat cgttagagaa gtagttaggg aggtccttat
     6421 cataaatagg gatatttatt aatgaatatt tgaaatataa ttgtatataa gaattaaaaa
     6481 gagtggggat agatatgaaa aagatgggta agtgtatttt atgtgaaaaa tacactgaac
     6541 ttactaaaga acatgtacca cctaaaaaat cagggaatac tggaggtaaa aagaccagaa
     6601 ctggtaattt agatgatttc cttaagagcg attttacaaa aggtgatttt ccgaagggga
     6661 ttaaaaggaa acctcaagga aatgtttatt ata
//
LOCUS       DAZULO010000119         6303 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000119 DAZULO010000000
VERSION     DAZULO010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.122"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            334..714
                     /locus_tag="ACG8OA_002549"
     CDS             334..714
                     /locus_tag="ACG8OA_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFS4977290.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="ACG8OA_002550"
     CDS             696..1610
                     /locus_tag="ACG8OA_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS4977291.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="ACG8OA_002551"
     CDS             1855..2544
                     /locus_tag="ACG8OA_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977292.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="ACG8OA_002552"
     CDS             complement(2665..2751)
                     /locus_tag="ACG8OA_002552"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS4977293.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="ACG8OA_002553"
     CDS             3427..4377
                     /locus_tag="ACG8OA_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS4977294.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="ACG8OA_002554"
     CDS             4398..4931
                     /locus_tag="ACG8OA_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS4977295.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="ACG8OA_002555"
     CDS             4947..5117
                     /locus_tag="ACG8OA_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977296.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="ACG8OA_002556"
     CDS             5567..6100
                     /locus_tag="ACG8OA_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977297.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            6112..>6303
                     /locus_tag="ACG8OA_002557"
     CDS             6112..>6303
                     /locus_tag="ACG8OA_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977298.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKS"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DAZULO010000120         6287 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000120 DAZULO010000000
VERSION     DAZULO010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.123"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..690
                     /locus_tag="ACG8OA_002558"
     CDS             190..690
                     /locus_tag="ACG8OA_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS4977299.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="ACG8OA_002559"
     CDS             787..2634
                     /locus_tag="ACG8OA_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFS4977300.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="ACG8OA_002560"
     CDS             2637..3533
                     /locus_tag="ACG8OA_002560"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4977301.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="ACG8OA_002561"
     CDS             3583..3972
                     /locus_tag="ACG8OA_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4977302.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6287
                     /locus_tag="ACG8OA_002562"
     CDS             3959..>6287
                     /locus_tag="ACG8OA_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4977303.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacg
//
LOCUS       DAZULO010000121         6243 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000121 DAZULO010000000
VERSION     DAZULO010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.124"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..288
                     /locus_tag="ACG8OA_002563"
     CDS             <1..288
                     /locus_tag="ACG8OA_002563"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977304.1"
                     /translation="TKVSSINTCSSYVQLRKSRYRGTFFFAAFFGLMRSTFAGRIRYK
                     TSFFTKDINRVKETSFCCQFFHFSKNENKKFWKWRRILLAILCLVEKRWTK"
     gene            362..1873
                     /gene="gadC"
                     /locus_tag="ACG8OA_002564"
     CDS             362..1873
                     /gene="gadC"
                     /locus_tag="ACG8OA_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HFS4977305.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            1886..3286
                     /locus_tag="ACG8OA_002565"
     CDS             1886..3286
                     /locus_tag="ACG8OA_002565"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFS4977306.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(3451..4891)
                     /locus_tag="ACG8OA_002566"
                     /pseudo
     CDS             complement(3451..4891)
                     /locus_tag="ACG8OA_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            5284..5877
                     /locus_tag="ACG8OA_002567"
                     /pseudo
     CDS             5284..5877
                     /locus_tag="ACG8OA_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
     gene            complement(5861..>6243)
                     /locus_tag="ACG8OA_002568"
     CDS             complement(5861..>6243)
                     /locus_tag="ACG8OA_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4977307.1"
                     /translation="FSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKG
                     CPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVG
                     YRNQRLAQRILDNELGCANASEAV"
BASE COUNT         1983 a         1026 c         1190 g         2044 t
ORIGIN      
        1 acaaaagtat cttcgattaa tacttgctca agttatgtgc agctacgaaa aagtaggtac
       61 cgaggtactt tctttttcgc agcttttttt ggacttatga gaagtacatt cgctggaaga
      121 atacgatata aaacgagctt tttcactaaa gatattaata gagtgaaaga aacaagcttt
      181 tgttgtcagt tttttcactt ttctaaaaat gaaaataaaa aattctggaa gtggagaaga
      241 attttgcttg ctatactttg tcttgtagaa aagaggtgga caaaataatg caagctcaaa
      301 taagcgattt tccataacct tggtaggaat gacaaattaa ataggaaagg gaagtaataa
      361 tatgaataaa aaaaagttat ctctattcgg tttttttgcc ctgacagctt ccatggtttt
      421 gactgtttat gagtatccga cctttgcaac atcacaatta catttggttt tctttcttct
      481 tttgggaggt attttatggt ttctaccagt tgctttatgt gctgcagaaa tggcaacagt
      541 tgaaggatgg aaagatggtg ggattttcag ttgggttagt caaactttag gagaacgttt
      601 tggttttgca gctatttttt tccaatggtt tcaaattaca gtaggtttta ttacaatgat
      661 ctacttcata ctaggagcgc tgtcgtatgt aattaatttt ccagagctta atacagatcc
      721 atttatcaag tttattggtg tattagtcat tttctgggta ctgacttttt cacagttggg
      781 cgggaccaat cgtaccgcta aaattgctaa agcaggattt gtcattggaa ttgttattcc
      841 ttcgatcatt ctctttggtt tagcagcagc ctactttatc ggcggtaatc cgattcaaat
      901 ggctattaat gcgaaagctt ttattcctga cttttctcag ctttcaactt tagtagtatt
      961 tgtatctttt atattggctt atatgggcgt agaagcttca gcatcacata ttaatgagct
     1021 ggaaaatccc caaagaaact acccattggc aatgttttta ctagtgattt tggccatcgc
     1081 cttagatgca atcggcggtt tctcagtagc agcggttatt ccacaacatg acttgtctct
     1141 cagtgctggt gtaatccaaa catttgaaac gcttatttta cattttaaca gtcatttaca
     1201 ttggttggta aaagtgattg ctctcatgat tgcctgtggc gttatgggtg aagttagttc
     1261 ttgggtagtt ggtccatcac gagggatgtt tgcagctgct cagcgaggtc tgttaccagt
     1321 tttcttccgg aaaactaata aacaagacgt tccagttccg ttgattatga tacaaggaat
     1381 tatcgttact atttgggcag cagtcttaac ttttggtgga ggaggcaaca atttatcatt
     1441 cttagtagca atctccttaa ctgttgtcat ttatctcgta ggatatttac tatttttcct
     1501 aggttacttt gtattagttt ttaagaaaaa agacttaaaa cgaacttaca atgtaccagg
     1561 aaaaactgtt ggcaaaacga ttatcggtgg aattggtttt attttatcag tgttcgcatt
     1621 gcttatctct tttgtaccac cagcttcaat tgcagctagt gagcaccgaa cataccaaat
     1681 catactactt attagctttg tagtgactgt tcttttacct tttatcattt atgaagtaca
     1741 tgataaaagt gctcacacaa ctattgagga acctactcac gtgaaagctg aagacgttaa
     1801 tcccgctgtt tatccagctg ctagaggaga gcaccacgtt acaaaaaatg atgaacatat
     1861 tttaaaacat taggaggaat tcattatgtt atacggaaaa gataatcaag aagaaaaaaa
     1921 ctatttggaa ccaatttttg gctctgcaag tgaggatgtt gacttgccta aatataagtt
     1981 aaacaaagaa tccattgaac cacgaattgc ttatcaatta gtacaagacg agatgttgga
     2041 tgaaggaaat gcgcgattaa acttagctac tttttgtcaa acgtatatgg aacctgaagc
     2101 agtgaaattg atgacccaaa cgttagaaaa aaatgcaatt gataaatcag aatacccacg
     2161 aacaacggaa attgaaaacc gctgtgtaaa tatgattgct gatttatggc atgctccaaa
     2221 taatgaaaaa ttcatgggaa cttcaacgat cggctcttca gaagcctgca tgctgggtgg
     2281 tatggccatg aaatttgctt ggcgtaaacg tgctgaaaaa ttaggtcttg atattcaagc
     2341 aaaaaaacct aacctggtga tctcttctgg ttaccaagtt tgttgggaaa aattctgtgt
     2401 atattgggat gtggaactga gagaagtccc aatggatgaa aaacatatgt caattaatct
     2461 agatactgtc atggattatg tggatgagta cacaattggt attgtaggta ttatgggtat
     2521 tacttacact ggtcgttatg atgatatcaa gggtctgaat gatttagttg aagctcacaa
     2581 taaacaaact gactataaag tatacattca tgttgacgct gcatcgggtg gcttttatgc
     2641 accatttact gaacctgatc tagtttggga ttttcaattg aaaaatgtta tctcaattaa
     2701 ttcttcaggt cacaaatatg gtttggtata tccaggtgtg ggttgggtct tatggcgtga
     2761 ccaacaatac ttaccagaag aattagtatt taaagttagt tacttaggtg gagaaatgcc
     2821 aactatggct atcaacttct ctcatagtgc agcacaacta attgggcaat actacaactt
     2881 tgtacgctat ggctttgatg gttatcgtga tattcaccaa agaactcatg atgttgctgt
     2941 ttatttagcc aaagagatcg aaaaaactgg tatttttgaa atcattaatg atggatcaga
     3001 attgccagtt gtgtgctata agctgaaaga agatcccaat cgcgaatgga cactatatga
     3061 tttatctgat cgtctgttaa tgaagggatg gcaagtccca gcctacccat tacctaaaga
     3121 cttggatcaa ttaattattc aacgcttagt tgttcgagca gactttggaa tgaacatggc
     3181 tggtgattat gtacaagata tgaaccaagc aattgaagag ttgaataaag ctcatattgt
     3241 ttatcataaa aaacaggatg tgaaaacgta cggctttact cactaaaatt cttcagagaa
     3301 atataaaaga agttaattaa ctaataaaag gagttgtgat taccacacag ctccttaaat
     3361 ctatttgaat aaatatatga tattcatttg ttacactctc aatcttagct gttgttctat
     3421 tcatcattac tcttattgta agtagttcag tcaaatttgc ttgttcaatt gatcgataac
     3481 agtatgaatt gtttgaaaag taatcggata tataattgtt ttctcactac ctaagccttc
     3541 ttggtataaa gaactatcgc aaagtataat ccgctgagta caaaagaaag tgtcgtctac
     3601 tatttctcta agttgtttac aaatgatatt actaatagaa aagtgttctc ccaaaatgtc
     3661 ttctaactgt tgtcgatagt ataaatacct aaagtctgac ggagctacta tgaacacttc
     3721 aattggagat ttccgtttat gctcatttaa aagacttaaa ctaatggtta gtttacgaat
     3781 agaattctca ttccaacgca gaaataaatt gttttgtttt cgccattgtt caagaatttc
     3841 atgaacttct aagtacaaag cgcgttgctc ttcatttaac aagtaagtct tatcaggttg
     3901 aaatatctga atatcagccc aaagtgagcg gataaacggg agaagtgctt tatcaaaaag
     3961 tatctgattt tcttcagata tattcagaga taagataagt ttttgactga gctcaatcac
     4021 caaagggtta tctgctacat ggttttgata aacaacgttc aagtcttttt gtaacaattc
     4081 aaagttatcg catagataat ttctagagtt gaatagaaag tagatataaa ggcattcatc
     4141 ttcgtcatga cataaagaga aagaaagttg ctctaatcct ttctgtatta gctgaaacaa
     4201 gggagtatgt cgggcaactc ttttttcttc ctgactaaaa tatattgggt tagtcttaga
     4261 tctctgcaaa ccgatagcga tacctaataa aataaattgt ttttcaactg tagggtaact
     4321 tcttgaaaaa aaatgttttt ctacataatc aatcaattgt tgaccgcttt gaataattga
     4381 tttatttagc tggatcaact ctgtccgatt tgtctcatag attatcgtta gtaacaaact
     4441 tcgataatcc ttttcaggaa tttcaaaatg gccatcagca tttaagtact gcatttgttt
     4501 aaaaaaatta atcaactcat ttcgtaagtt gtaaactttg cttaacgaaa tgaaggtagt
     4561 atcagctata ttactccaat tgtgttcccc tgataaataa tgtcctagta cattgaaaaa
     4621 gtcagagcgc tgatagatga gttgagtgag tgccaacctc gaaaacgaag agtcgaatgc
     4681 gactgtaaag ctacttctag atttctccac tgaaataatt tgctgatgga gtatgtcggt
     4741 aatgcattcc aagtcgttag tgacagacag tgagtgggca aacgttcaat tgatctgcta
     4801 tttcgcgaat ctgaatggtc cctttttcgt gtaattgctc acaaatgtaa acttgacgaa
     4861 aaatactttt ctccagatag gtttccaaca tagtactcct cctagtcatt ttatacataa
     4921 ctattataac tctaccaatc ggaatagtcc tgtaatatat tgaatcaaaa atataaatta
     4981 taaaataatt agatggggta tttggtttaa gtatccctta tatagtaata aatttatagg
     5041 tgtgattctc gaattaatac ccgaaaatgt gagagaattt ttatggattt tttaggatac
     5101 aaattaatta tttttgcttt tatcctgatt accagtccag tactgtcggt atcccatttt
     5161 gcagcaaaat catgttgata aagtgacaag aagactgatg agatatatta tccaaattga
     5221 gaagataatt aatcggattc ttgagtatac cctacttttt aagaaagtag ggtatacaag
     5281 cgtatgaata aagacataac tttccctgtg atggacgttc ggcttgttcc aattaccaaa
     5341 atacaggcga acgattataa tcccaatcga gtagcgtctc cggaaatgaa cttattggag
     5401 ctttctatca ttgaagatgg ctttactcaa ccattagttt gttattacaa ggaaaaagaa
     5461 gagaagtata tcttggttga tgtgcttcat aggtatcgtg ttgctaagga aagactcaac
     5521 ttaaaagttg taccggtaac agttattgat aaacccatat aagaacgtat ggcttcaaca
     5581 attcgacaca atcgtgcaag agggacccat gaaataagag gaatggaaat tgtcatccaa
     5641 agattagtaa aaaatggatg gtcagacaag agaatatcca aagaattagg catggacatt
     5701 gaagaaattt ttcgctttaa gcaagtgtct ggtggaatgg caacagtttt tttgacaaat
     5761 tttataaggt gcagaacttc tttccgtatg ctattccgat tgtccttgac aatgagcctc
     5821 ctcggatgtg acgatcttcg gcaggagcta acagttaaag ttagactgcc tcgctagcgt
     5881 tagcacatcc gagctcatta tcaaggattc gctgcgccaa tctctggtta cggtaaccaa
     5941 ccggtgtctc gtagccaagt gtaccgtgca accgaaggtg gttccaccaa tttacatagt
     6001 caaataactc caaatccaat tgttgtaagg tttcaaatgt gtattgatag acaaattcta
     6061 ctttcaacga cttataagtt gattcagcta cggcattatc aaaaggacag cctttatgac
     6121 tcaatgatcg attgatgtca aaagttgtta ataattcatc aatagcttgg ttatcaaact
     6181 cttttccacg atcagtatga aaaatctcaa cctctgtcag aggttgtttg atacggctaa
     6241 atg
//
LOCUS       DAZULO010000122         6235 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000122 DAZULO010000000
VERSION     DAZULO010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.125"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..1189)
                     /locus_tag="ACG8OA_002569"
     CDS             complement(59..1189)
                     /locus_tag="ACG8OA_002569"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFS4977308.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1248..1475)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1837..3039)
                     /locus_tag="ACG8OA_002570"
     CDS             complement(1837..3039)
                     /locus_tag="ACG8OA_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4977309.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3099..3302)
                     /locus_tag="ACG8OA_002571"
     CDS             complement(3099..3302)
                     /locus_tag="ACG8OA_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS4977310.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3444..4511)
                     /locus_tag="ACG8OA_002572"
     CDS             complement(3444..4511)
                     /locus_tag="ACG8OA_002572"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4977311.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4569..4676)
                     /locus_tag="ACG8OA_002573"
     CDS             complement(4569..4676)
                     /locus_tag="ACG8OA_002573"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4977312.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4839..5651
                     /locus_tag="ACG8OA_002574"
     CDS             4839..5651
                     /locus_tag="ACG8OA_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4977313.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            5806..>6235
                     /locus_tag="ACG8OA_002575"
     CDS             5806..>6235
                     /locus_tag="ACG8OA_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4977314.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT         1880 a         1253 c         1012 g         2090 t
ORIGIN      
        1 ctccactcca tgaaaagccg actttttggg acgctttttt ttgccaagcc tcacattttt
       61 atagccaaac ttcttttgcg acttgctgca ataattggat tttcttccat tgttcttctt
      121 ctgttaaatg gtttccttct tctgttgaag caaacccaca ttgaggactg atacttagat
      181 tttccaatgg tacaaattgt tgtgcttctt ttattcgttc ttttattttc tccgtatctt
      241 ccaaatctgg aaacttagaa gtaactaatc ctaaaacaac tttactttct ttgttactcc
      301 aatgtcttaa aggagtaaaa tctcctgctc ggtcagaatc gtattccaaa aagaaaccat
      361 cataatttgt tgaaaacaac tcttctgcaa tgggttcata tccaccggaa aacagccaag
      421 aagaagcgtg attcccgcgg cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt
      481 ccagtgcgtt attgataatc tctgtacaaa tatgggccat ttctttcgga tcaattcctt
      541 tttgaagaag tgcttctctt ttagtttgat cagctagaaa cgcccaattt gtatcatcga
      601 attgcaggta acggcaacct ttttcataaa aagcatgaat cgctgctttg taagtagagg
      661 acaagtcctc ataaaaatca gatagatcag gataaatctc cttgattttt gaagaaccat
      721 cattagatag gatctcttgt cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa
      781 caaaagtatc atctgtttcc actacttctt tcagaaaagc aaaatctttt aaaaatgggt
      841 gatttggatt gaaagataat ttatctacta cccgaatatt ataagctggt gctgcttttc
      901 cgtggaatgt ttgattatat ccagtttctg gaacatagcc ttcgatacca tttagttctt
      961 ctaaaaaatc aagatgccaa aatccacgcc gaaattcacc atctgtaatt cctttgatgc
     1021 cgactgcttt ttggtcttcc actaattttt ttatttcctg gtcttcgatt gcacgaagtt
     1081 cttcttttga aagtaagcct tcttgaactt tttttcttgc ttctttgatc gattgcgggc
     1141 gtaagaaact tccgacatga tctactgtaa atggtctatt tttgctcatt tcactcactc
     1201 ttttctttgt tttttgtaaa aaaaatcctt ttactagcaa atactagtaa aaggacgatt
     1261 atactcgtgg taccaccttt tttcagaaaa aacattcttt ctctcagcct gacaataaaa
     1321 tcagtggtct tggtaacgaa agactgttcg tttttgccta cctttcgaca aaaaatcctc
     1381 taaggccatt ttcgacagtc aacttctagc tttttacacc aaccaaagcc tctcttgaac
     1441 cattttctgt ctactttcct tctcatcgga tactattaga ataaaattag aatagcatgt
     1501 ttgaaagaaa agtcaatagg gaataagaac aaacaaaaac taatttgtta ttcttagtaa
     1561 tttgtttgtg gaatacaata aagcttttct aatatgataa ttgttcagtc gtcctttcat
     1621 cttagatcac ttccttaaaa tgatcatacc cgtttcaacc acataaaaaa ggacttaatt
     1681 ttgaaaaatt tcccatcaaa actaagtcct ttttattata gttatgcaaa tttgcttatt
     1741 ttctcgctat ttccgataca tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa
     1801 agcatttatt tgtcttatgg tagaaccgtg gaaaactcag actggtaata aattgtcatt
     1861 ttttgaaaga atagcgccct cttgaacatt atcaatcgca cttaccactt gttgatctga
     1921 ctttgcatat aagtgaccgt aagttcctaa agtgatttga atattcgcat ggcccaatcg
     1981 ttcttgaata gatagcggtt gaatattctg ctcaattaga aatgcaacat gagaatgtcg
     2041 tagcgcgtga agcttaatcg gtttgacacc cgctttttta gcagcttctt ttaaccagtt
     2101 cgcaaaagaa taaggtggcg taaatgttcc gtccaactga gcaacatatt caaaatttcc
     2161 aatacgtttt tgcaagtcat accactgtct gagttttaag gaaagcttct tccccaaccc
     2221 aatcgttcga taagcacatt tagttttggg agtcgaaaag taccaatcat cttttgtatt
     2281 ataaatcaat gtcttctcaa tcgttatcgt gtttttctca aaatctacat tcgaccaaga
     2341 caaagcttca agttctccaa tccgaatgcc agtataaaag agcatttcat acacaatctt
     2401 ccgatggtgg gtttgaatct tatcatcctt caattcattc atcacttttt gaaactcact
     2461 gtgttcccaa aactcaactt tcttattctc agaaggaagc gaagaaacat cttcaacggg
     2521 atttttagca atcaccccct ctttaacagc ccgatccaat acaattttta ggtgccctcg
     2581 aatccgattg atataagatt ttgataacgt ttcgccatta acttttttcc gtgaattacg
     2641 tgactctgtc tggcttagtt caaataacca atcctcaata tcgtgacttg taatatctcg
     2701 aacacgaaaa tgatagaaaa actgcatacg cttttcgaaa atgaattgcg cattctcata
     2761 tgtttgactt ttgacctgtc tctcgtacca ttttacaaac ctatccttgt aaaactcttc
     2821 gaaagtcatt tgattggtag aacacagttg attaggaatt gtcttgttaa aatcatgtgt
     2881 ttccatgtac ttgtcataag caattttggc atctttttgt gaagcataac ctcgtttagt
     2941 cttctgaacc tttcttccat tttcatctat acctagatac gctcggaaga accatttttt
     3001 tgtcttttta tcttggtaaa tatttttaat ttttggcatc gttccattcc tccctgtact
     3061 tgagaaaagc tactttaata gtaccgcgct tgtactgttt attccgcaac ttgaatgtga
     3121 aaaagatgtt caattactcg ggatggcacc acaccaactt ggcgattatt ataaaaatca
     3181 ataccttcaa tccttgcaag atatgccttg ctctctttaa tcattcgtgc agcttgacta
     3241 ggtttaaagc ccatctctat caaaattgca cgatttacca tgaaatcaca ttttgtttcc
     3301 attctctcac ctcctgattc tttactcctt ttaaaataat cctctttata atctattgtt
     3361 tctcattcga tggttgtgtt atactcgtct tgtcgaggcg gagtacgttg cacaacttgt
     3421 tgtgtaagca gtactttgct tttttagtag cttgttttct catttgatat actttgtaat
     3481 tttcgttcat aatactcaac ctcctcctga taatactccg ggtctgcctt catattttgg
     3541 agaagtttga tctgtcttgg attcatttct ccttgttcaa taatcaaagg caacaaggaa
     3601 gaatgaaacg tttgatcgac ctgtgcaata aaaactaatg atggtgaaac accatgaatt
     3661 aaccaactca tagttttctc aaaactttga ggaattgtat ctaaagaaat ggtgattgcg
     3721 ccataattgc taataaaggc tgcccatttg gcatcaactt ttgcacctag atcatttgga
     3781 tcacggtcat aaaaacaaat tgctccatta agtaactcaa agaacagttt ggttaaatca
     3841 tgggtttttg aaataatctt tgccaagctt tgcgctttct cttttcgata ccgtagttca
     3901 aagcgattct ttacttttgc ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg
     3961 tccttctgat aaaagcaaaa acgacactga ctcttctttg agcctaaata caaggtctca
     4021 ccatttgatt cattgtttat gatatccgta ccgccatgag attcactcac ttgaaaagtg
     4081 gtccacacat ggcctagtat taccttatgc ttaagttctg gaatgctgag actccccaca
     4141 aaatcatcta aagtaaaatc aatccgtgta aagttcccct gataatctaa taccttacga
     4201 aaaaactgtt gccaatttgt ttttcgtgtt tttagccatc ttgctaagtg tttacagcct
     4261 tgacctgaaa actccatcac agttccttta accgcatcgt caatggaata ccgaatcgtg
     4321 attacttgat tccacacgta ttggccaata tagccctttg gcgcataatc cagttcctca
     4381 aaacgttcaa tgggcatttc aataatctct tcaattagct ttaaatgttg attcgagaaa
     4441 aagtgaatgg ttagataatc aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa
     4501 ctatccaaca caagattcaa tttttcttca cttaattgtc tcgttccttt ttctactttc
     4561 gataataact atctcgaaat ccccactttt tctgctaaat atcgttgtgt ccaccccctt
     4621 tcttctcgag aaactcgaat ggcttcaccg agatcatgtt gattaacttg gttcatcgac
     4681 aaacctcctt ctgctattta cttgtcaaag aacctattga acttatttta agtacatttt
     4741 tattgtacgc ctgtatttat tttaagtcaa tatgttttga ctttttttaa gtacacacgt
     4801 atactaaatt taagaactta tcctgaggag gagttttcat ggagatagac aagcaagcag
     4861 tcggcaatcg aattcggcag attcgccaag aattaaagtt gagtatggag aaatttggca
     4921 aactaattgg tgatttaccg cgaagtacag tcaataactg ggaacgtggg atcaaccttc
     4981 caaaaaccga aacccttcac caaatcgcag agataggtca tgtcaccaat gaatatcttt
     5041 tgtatggtga tcaagagaat caatacattt tagaaatgct ccaaaaaaag gcaggtaagc
     5101 ttgatcctaa gattgaaggt ctcatagtag acgaaatcaa acacgctgac cttgttagtg
     5161 aaaaagaaat ggatcgaatg attgaatttt ttattacaaa tctcattcca ccaactgaac
     5221 aagaccagtt tactttccaa tgtatcgatg aaaaaaagca gctttatctt ggttcaacta
     5281 attttggtaa ggaagcccag ctctatttgc aacatgataa tcaaaatcat attctgcaca
     5341 tgatgccctt taccttctcc aactttagtg tggatcgcat actcgtctat ttagcaaatc
     5401 aaaaatccta ttcctatttt gggaaacatt tacccaagga actaggtgag aaagccatcc
     5461 ttctatactc aattaatcaa ttgaccagcg atgtgcgaat tgccccgttg gtctattcag
     5521 aagaaaccgc ttcttatcag tacacagagg acaatcaata tctactagaa cagcaatatc
     5581 tttatctacc attcgtgatg gaagtcgaga aagaacgcct gttaaatgca gcgtatccat
     5641 catcaaaatg aggtgacaaa attgataagc acgtgacaaa gaaaatactg ctaggacact
     5701 ccccaatggg tagttgtcac agtttcctgt tctttgttag ccccctgtta gatgatgtgc
     5761 accccaaaag ttagactaga aatctaatta aaaggggtgc tttttttgcg gaaatataca
     5821 tttgttttta agaaaaaagt agtttcagac tatttaaata acgaaggcgg ctacaaatat
     5881 cttgcacata aatatcaaat aaatcgtaca ctggttagac attgggtaag gatttataac
     5941 tatcatggtt gggaaggctt ggttggaggt ggcaaaagct acactacaaa atttaaactt
     6001 gatgttatag aatatatgga aacaaatggt ctttctatcc aagaaactgc taaaaaattt
     6061 aatatcggtt caaatagaac tctaagtaaa tggatagagc aatatgaaga aggcggtgct
     6121 tcttcacttg agagccaaaa aaggggcaga aaaattagta tgaattccaa gctaaacatt
     6181 cctaaaaaac ttaaagatga gtctcttgaa gaagaagtta ttcgtttaag agcag
//
LOCUS       DAZULO010000123         6173 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000123 DAZULO010000000
VERSION     DAZULO010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.126"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACG8OA_002576"
     CDS             725..811
                     /locus_tag="ACG8OA_002576"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS4977315.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACG8OA_002577"
     CDS             complement(932..1621)
                     /locus_tag="ACG8OA_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977316.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="ACG8OA_002578"
     CDS             complement(1628..1906)
                     /locus_tag="ACG8OA_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977317.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="ACG8OA_002579"
     CDS             complement(1866..2780)
                     /locus_tag="ACG8OA_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS4977318.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACG8OA_002580"
     CDS             complement(2762..3142)
                     /locus_tag="ACG8OA_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFS4977319.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACG8OA_002581"
     CDS             complement(3592..3879)
                     /locus_tag="ACG8OA_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS4977320.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACG8OA_002582"
     CDS             complement(3899..4123)
                     /locus_tag="ACG8OA_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS4977321.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACG8OA_002583"
     CDS             complement(4578..4748)
                     /locus_tag="ACG8OA_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977322.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACG8OA_002584"
     CDS             complement(4763..5296)
                     /locus_tag="ACG8OA_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS4977323.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACG8OA_002585"
     CDS             complement(5317..>6173)
                     /locus_tag="ACG8OA_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS4977324.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1633 a         1152 c         1042 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaacaaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAZULO010000124         6122 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000124 DAZULO010000000
VERSION     DAZULO010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.127"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(451..1233)
                     /locus_tag="ACG8OA_002586"
     CDS             complement(451..1233)
                     /locus_tag="ACG8OA_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977325.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1298..2134)
                     /locus_tag="ACG8OA_002587"
     CDS             complement(1298..2134)
                     /locus_tag="ACG8OA_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4977326.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2152..3561)
                     /locus_tag="ACG8OA_002588"
     CDS             complement(2152..3561)
                     /locus_tag="ACG8OA_002588"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4977327.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3575..5398)
                     /locus_tag="ACG8OA_002589"
     CDS             complement(3575..5398)
                     /locus_tag="ACG8OA_002589"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4977328.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1907 a         1309 c          976 g         1930 t
ORIGIN      
        1 cccttttata aaaagcaaaa aaaaaaagaa agaaacttat tcttgctaat aaactacgcg
       61 aaaggagtct aacgttgttt gatataactt agactgatcg ttaacttatt ttttatattt
      121 ctacatagtt atctttagaa ccatttcaga tacatatgaa acaattacat atgatttttt
      181 cataaaaaaa cagtactgtc tagtagtata aaatatacaa atttcttaaa aattttttca
      241 ctaaaatcac tacctgaaac agtctaaaac acttatatat caacgtttaa tgaaactatc
      301 tccgtttcat ttcagataca aataaatata gtcgggctcc aaatccgaaa aaaaataccc
      361 ccagtagctt atcctaagct actggagatg tataagtacg gtcatataac aacggggtag
      421 ggtaaataaa tgaaaaagat ggaagaatca ttattcaata acttttttaa atacttcgtt
      481 gtaatcagtt acctgtccgt tcttaaatac cacagaataa tcttttttcc cattttcaag
      541 taaccgaagt ggaatgtctt taccaacttg ctctgtcact gcattactga tacctgttag
      601 attagctaat aaatctgata aagattgagc atctgtagaa atctctgtat cagtatttat
      661 cgtgattcct ttatcttcat ttacaagctt ccaagtaggt ggtatagctg tgtatgtttc
      721 gatgaatgaa tcaattcttt catctatcgt cttcccagta tctacttctg tctcattact
      781 cttagatgaa tcgattcttt catccatcgt ctgctccgta actgcttctg tctcattatt
      841 ctcaccggaa actctcttca cacccaaact tatatattgt ccatctttag tgtgcatagt
      901 taaaattccc acgcttttac taataaattc actataacca gttctttcaa tagagacttc
      961 aatgtatttc acactatcat actcaaaaga aaaatagaca tgtaaaagca ttttgttgtt
     1021 tacatctaca taataatcct ttgactctac ttgagacctt cctatacgga atacagcttt
     1081 actgtctgat atctctatac cagcatcctt attattaatc tgccaatctc caagtagctc
     1141 ttctggcaag ctagtagttt cttgtgaaca ctttaccact tcactggtag aggtcgaaac
     1201 attcacctcc gcactgttag aagaattttc caaggaatca ggagccttag cacaaccaac
     1261 aagtctttat atattgtgca gccgatattt tcttcattca gtacaccctt gaaatatgca
     1321 gtgttagata aagcagttcg tcctgatttg ctgaggcacc catctcttct tgaacgtact
     1381 ccttgatttt caaggccgcc cgatactctt tcggaaaacg ctgcttcact gaagccagca
     1441 aaaaatcatc cttctccggc aactcttcct tcttaatcat tcgggcaata aaaaactgta
     1501 aatgcgtcac aaatcttaag taaagcatcg aatctttatc aacctttttt tcgggaaaca
     1561 gatatcctag catagccaag atatccttta gtgctcttac cgagagcatg gtttcttcca
     1621 tatttgcttg ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca gcctcctctt
     1681 ctggcaactg aacattcatt tttttgttga tcaattggat agcatagcag ccgatctcaa
     1741 attcttttgg gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa acaatgcctt
     1801 tttgactgcg ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat agacttttat
     1861 tcagcttaaa gtcaaacttg gcgttggcat aatcgataat tcgcttgacg caaataatta
     1921 gatcatccgg agcagcctca aacagtttaa tgtaatcctt ttttagagca tcagaaagtg
     1981 aatctaattt aaatactttt tggattttgt cagccggaac caaatcgcct ttctttagtt
     2041 gaaatccaat ccctttcccc atcaaaatta cttctttgcc ttgcttatct aagcaaagaa
     2101 ttaaactgtt gtttaacgtt ttaattactc tcattagatt caccgttctc tctaagctaa
     2161 gccatttctt gcgattactt tttgatacca aaagaagctt tcttttttta ttcgttttaa
     2221 ttctttaaga ttctcatcgg ttcggtcaac atatataaaa ccataacgct tattgaagcc
     2281 ttgatgggta ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa catctacacc
     2341 atctgttaaa gctgcttgaa tagccagaat atgtttggct agataatcaa ttcgatcctg
     2401 gtcattaatt cgtctatctt cctcaactat gtcgggtgcc ccatagccat tttcagtaat
     2461 taaaataggt aaatcatagc gatcgtacaa tttcctcaat gttaatcgta gtccagtagg
     2521 gtcaactacc cagccatatg gcgttttatc tacataagga ttttcagctg cccggtagac
     2581 accttcttct cccaacatga tctgttgatc ccccgctcta gcggaaacat ctgatccgtc
     2641 atttttgctg gcagcgatgg tggcagttga ataataatta atcgcaataa aatcgggttt
     2701 accggaggat aaaattgcct gatcctctgc ctcaatttta ggatataatc cacgatcatt
     2761 caaatagttt tcaaacaaat aattgtattt tccccgaact gctacatcta agaaagacca
     2821 gcctctcaat gtttcccaat tatgggctgc aattgcatct tctggactgg aggtagcttg
     2881 atacatagcc gttaaattga ttgctggacc gatttttcct ttaagattca actgatgata
     2941 caagtgaatt actctagctt gagctaataa aacatggtga ttaatttgaa agagttcttt
     3001 ttttgaaggc agctctccac ttggtggcaa accaatcgct ccaggatgaa ggatcatcgt
     3061 gttttgctca ttaattgtca gccaatagtt caccttgtca ccatactcag taagcaatac
     3121 ctgtacatag tccacaaaat aatcgatagt tgctcgattt aaccaaccgc cctgctcctc
     3181 tagaaaataa ggtaaatcga aatggtacat ggtgacaatc ggttcgattc cctgattcag
     3241 gcattcatca attacttgat gataaaaaga aattcccttt tgattggttt tattattttc
     3301 aatatccata attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc ctaattcttt
     3361 aaacagctgt atatcttctt tatagtgatg gtaaaaatcc gaggcaactt caaaattagc
     3421 taccccttct gtatgctgat acttgtcaat aatagacggt tgtttcccat cctcctgata
     3481 ggcaccctct gcctggtagg cactaatgga ggcaccccat aaaaacttct ctggaaatgc
     3541 tgaaagctgt ttgtattgca caaaaatcct ctccttaact aatttgcatc aatggttcgt
     3601 gttcctttaa gcttccgctt gcttcactaa ttcctgcgta attttcactg ttggtcacta
     3661 ccacaatcac tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg gcgttgatta
     3721 ataattgtcc tgctgacacc tgatctcctg cttttacaaa tacttcaaag ggttcccctt
     3781 ttaaattcac ggtgtcgatc cccacatgga tcaataattc aataccttct ttagaacgta
     3841 gaccaatcgc atgtttagat tctaaaatag taatgacttc tccctcaaca ggtgaataaa
     3901 tgctgttttc ttctggatga atggctacgc cctttcccaa tagtccctta gaaaagacag
     3961 catcgttcac ttcagaaata ggacacacgg ttccttttac cggattttca acaatgactg
     4021 cttcggctat tgccgtttgc tctgattcat cagaagtatc gtcaaatcca attagataag
     4081 caatgataaa ggttgcaaca aaggcgatta cacacgtaat aattgcgtga acaatattca
     4141 ttggattttc tccaataaag gctggtaagg ctgctaagcc aggggaaaca aaggcatatc
     4201 tgactaaacc gctcaagcca gcatacaaac cagcaattgc accaccaatc atggaagcaa
     4261 ctaatggttt tttcagcttc aaggttactc catagagtga aggctcagta attcccatta
     4321 aagcagtgat cccagtggac acagctagct gcttcaactc tttgttttta gttttcgcgg
     4381 ctacagcaaa ggtagcacct gattgagcaa tattagaagc cagcattcct ggcccattga
     4441 tcatttcaga acccgtactg ctcaattgca tagtagcgat tggcgtcatt gcccaagctg
     4501 tacccgtaac caccaaaaat ggctgcaaac cacccatcaa aaatggaatt aaccaagctg
     4561 ctttgccgtt aatccattca gcccccattg ctataagatc gttcagcaac gttccaaacg
     4621 gtccaatgac aaccaacgct aaaggcccca taattaaaaa taccagcatc ggcttagtaa
     4681 agaatttaat aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag gacatgatcc
     4741 aaacagtaaa gataatcggt aaaactgatc ccgcataatc tgcagctaat accggaattc
     4801 ctagaaaaga aacggcatcg ccagtagaaa aaagctgtga aatatttgga tgaagcaaaa
     4861 tgccagcaac agtaatcgcc aaaattgggt tgacatcaaa ctttatggct gccccatagg
     4921 ctaatagtac tggcataaaa taaaaggctg catcagcaat agtattcaaa atgatgtaag
     4981 tttgactact gtctgatagt acaccagtca aagtcaagac ggccaaaatg gcttttacca
     5041 tcccggcacc tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg acactaatca
     5101 atcttgaaat aatactttct tgattgtctg aagaggggcc ctttttaatt gaagttccca
     5161 cttccttttg aatttccttg aaggctgttt ggacttcatt gccaatcacc acttggtatt
     5221 gtccaccgct atccaccaca ctaattacac caagtgtttt ttctaaggct ttcgtatcgg
     5281 ctttcctttt atctttgagt tcaaaacgca gtctggtggc acaatgggtt aagctgacca
     5341 cattttcttt tcccccaacc tgttgcaata catttcttcc tacttctctg taatccatta
     5401 ttctctctcc ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat caacattgaa
     5461 gtgttagttt gtcttggtct tgcctgcata atcagtaaca tgccaataac gttattttgc
     5521 tgtaatccaa taatagcaca ttatgatagc gcaatcaaca gggaatttaa ttttttatct
     5581 tttcgtttga ccgtataggg taatataaca gttagatcac atcaactagg gtatatctgc
     5641 gtatacttac tgctaaaata agtatatcta atattaacta taccagtaaa accattccta
     5701 cataaccagc gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat aacggatcac
     5761 acaacttcaa caattctgct cttgggaaaa attgatgaat ttggtgagta aaggatggaa
     5821 taaacagcat tccaacaata taggcaacaa tagtgatact tgtttttttg gtaaaaatca
     5881 gtaagcaata gccaatttca atcattccga gatataaagg tatcgataga atgatttggg
     5941 ctagtattct taaaaattaa ctagcaaatg tttaaactga tttatttcta atctacatgt
     6001 agtaagtatt tcaattccac caattatcaa taaaaatacc aaacatatcc ataataattt
     6061 tttctttttc atttctttta tattgttata acagtgaata acataaaaat tcccgacaat
     6121 ca
//
LOCUS       DAZULO010000125         6002 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000125 DAZULO010000000
VERSION     DAZULO010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.128"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..395
                     /locus_tag="ACG8OA_002590"
     CDS             <1..395
                     /locus_tag="ACG8OA_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS4977329.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="ACG8OA_002591"
     CDS             398..1228
                     /locus_tag="ACG8OA_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFS4977330.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="ACG8OA_002592"
     CDS             1244..2095
                     /locus_tag="ACG8OA_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4977331.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="ACG8OA_002593"
     CDS             2092..2451
                     /locus_tag="ACG8OA_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFS4977332.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="ACG8OA_002594"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="ACG8OA_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="ACG8OA_002595"
     CDS             2624..2929
                     /locus_tag="ACG8OA_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977333.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="ACG8OA_002596"
     CDS             2929..3240
                     /locus_tag="ACG8OA_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFS4977334.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="ACG8OA_002597"
     CDS             3237..3410
                     /locus_tag="ACG8OA_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977335.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="ACG8OA_002598"
     CDS             3407..3823
                     /locus_tag="ACG8OA_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFS4977336.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="ACG8OA_002599"
     CDS             3823..4491
                     /locus_tag="ACG8OA_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFS4977337.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="ACG8OA_002600"
     CDS             4488..4865
                     /locus_tag="ACG8OA_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977338.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="ACG8OA_002601"
     CDS             4862..5161
                     /locus_tag="ACG8OA_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977339.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="ACG8OA_002602"
     CDS             5151..5309
                     /locus_tag="ACG8OA_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977340.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="ACG8OA_002603"
     CDS             5403..5819
                     /locus_tag="ACG8OA_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFS4977341.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2231 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 ga
//
LOCUS       DAZULO010000126         5914 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000126 DAZULO010000000
VERSION     DAZULO010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.129"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(525..1028)
                     /locus_tag="ACG8OA_002604"
     CDS             complement(525..1028)
                     /locus_tag="ACG8OA_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS4977342.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1387..2412
                     /locus_tag="ACG8OA_002605"
     CDS             1387..2412
                     /locus_tag="ACG8OA_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4977343.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2735..3613
                     /locus_tag="ACG8OA_002606"
     CDS             2735..3613
                     /locus_tag="ACG8OA_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFS4977344.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4089..4205
                     /locus_tag="ACG8OA_002607"
     CDS             4089..4205
                     /locus_tag="ACG8OA_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS4977345.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5133..5471
                     /locus_tag="ACG8OA_002608"
     CDS             5133..5471
                     /locus_tag="ACG8OA_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977346.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5565..5795
                     /locus_tag="ACG8OA_002609"
     CDS             5565..5795
                     /locus_tag="ACG8OA_002609"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFS4977347.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         2010 a          888 c         1082 g         1934 t
ORIGIN      
        1 aataacatat ctcattttac gcttttgtta ttttatactt aaaaaataac gacacagaaa
       61 ataccgtgtc tcagatttct ctactacttc taaaaactac tgtttttttc ctttgaaatg
      121 ccttcaaaaa gtaggatcag gcagaatata ctggcagaat caaggagaag ggagcctaaa
      181 taaggagaaa aaccatagaa aatataaagt aattacttat aaaacaagct ttttatagaa
      241 aaataatata cctgagtagg gtataaatta agacggaatt cctttctaga ttgagatatt
      301 gactaggaag aatgttaaga ttttaagcct ttaagctaaa aaaaaaccct ttttgttaaa
      361 taaggggaat atagcctgtc agtttcaaac tgcaagtcgt cctgctgcaa taatatagta
      421 gattagagat gaaaatagat ttttttaaac caatgactga ataaaaagac cgtcatagat
      481 acacattgga atgtgtctat tacggtcttt ttttacgaac gattttattg aagacggaca
      541 gttcgggtca aaaataaaaa agctacgatc agaaataaga ataaaatgta tcccaaaata
      601 atggctagac ggtctaactt atttgtcgat agatgtttgt ttagaagttg ataggcccat
      661 aaacctatca gtcctcctgt tgtattagtt atcaaatcag tgatgtccgc tacgccaata
      721 ccgaatagga attgaatcaa ttctgccaag aagcttacaa ggaaaataat ccctaatttt
      781 ttagaaaaag aaagtctctt gaaattgaca ttcaaaagca ccccaaaagg aaaaaagaca
      841 atcaagttat aagtggtctc acgtaatacc gtcgtatttt caaaagaaaa agtagaaaaa
      901 gggacgagat tgacattgct gtaagaataa gtgagaaatt gtggaagatt aaatgatagc
      961 ttgaataaaa tcaaccataa tagaaagaat aaataaatcc ctaataaaaa ttttgataca
     1021 taccgcatag acatctcctc catttaaaaa gtataccatc gaaaaccgct gaaaaatcat
     1081 aaaaaagtat gaagtttaag gaccaacgta gttatcgtac gaaaattaga agcttttttt
     1141 tgttgggaat ccggtttctt taacaaagct aagatagcga gttgcttgga aaagttgcta
     1201 tcttttttct gtcaaaacga ctcggaatgt tcgttttttg ttctaatttc tccaagttgt
     1261 ttactttttg atactaagtt tatgaaaaat caagaaatag ttgttatact aggtaatgct
     1321 gacaaacggg attggcattt catcttggtt cagctttttt tccaattact aggaaggagg
     1381 gatagattgt tcaagactta ttttcgtttc aataaaatac aagataatcc ttttcgtatc
     1441 atgaatctga tgctacttat ctttattgta ttatggttag gttacataaa tcatgatttt
     1501 ttgatttcaa gtttttcatc attagggatc tttacttttt tctattatca aaacataccg
     1561 atgaaatatt tgctgaaacg tatgtttctg atcggttgcg gactgttggt tgctttcgtt
     1621 ttggggatct tatcgactta tgttttatgg cttgaaccat ttgccgtagc tttggttgct
     1681 ttttccagca gattcgtgtt gcgattattc catatttcta aaccaggtgg cttgtttttt
     1741 gcgatgcttt ctgccatggg aacgagcatg cagctgccaa tcgctcaatt accgatcgtc
     1801 tcactttatt tttttatggg tgtcgttttt gccctgattg cggcagtgat tacaaaatta
     1861 ctggattcac ggcctgagca gacaattgaa aaagcaactt taaaagaaag atttcacgaa
     1921 gagcctttag tgatcattga ttctgttttc tacagcgctg ccttatttct ttccgtttat
     1981 gtcagtcacg gcttgaatct gcataatcct tattggttga ccttgtcatg tgcatcgatt
     2041 ttgctagcag aaaacttgga tgcgatgaag catcgacaag tccaatatct gattggatcg
     2101 atgggtgggt tatgtgtgtc ggcattcttg tcatttgtgc cgtttacgca gttgcagacc
     2161 atttttctga tcacttttct atatggaatc gcacaatttc tagttgctag aaattatgca
     2221 gttgctaata tttttcttaa tccgatggcg ttgatgctat ctacacttat tcgtggagca
     2281 tacttgattt ctttgatcga atatcgtttt ttagggatcg tcataggaag tttcatcggt
     2341 ttaggtgttg cgtgggtaat gacggttggt ttgcaacatt atttaacggt tgttcgtcaa
     2401 aaattagaat agaaggacaa atatatgtgc caaaagaggt tatgacaaga tttttgtcac
     2461 aacctctaat tcaaggtgtt caaaagccag attctcagca ttaagtcaaa ttcacacaaa
     2521 aatgtggaaa ggaactttca aaaaaatttt atatttattc tatcaaaaaa ttttgtttat
     2581 gatggtaact gaataaactt tcctgagatg gtcaatgctt tccttgaaag tcttacagat
     2641 attctttctt catagaaagt ttaagtttta tagttagtgt acaataaaag tatgtttata
     2701 ccatatggtg tccggataat cagtcattta aacaatgagg ttcaaagata tgaaaagggg
     2761 aaatttgatg aagaaaataa gcaatgaaat ccaaattctg gcagaaactt taactcttcc
     2821 caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac atccttgatg aggtgtttga
     2881 gcaatatagt catgaactaa tggatcctaa taaagcaaag gcgactgcaa agaaaataga
     2941 caaagtctat gaagaggaac cttttgcagc attagctatt tatctatatg cagcttctca
     3001 aagttggaaa aaaatttatc aaccattgaa gattccaaga actatctatt tggcaacaat
     3061 gaatgctttt actagattta ttcatgaaca tttccaagta acaggaagct atcgctttga
     3121 ccgtggtttt tggatttggc attacactag tggcttgatc tttcggattg gtgaattgga
     3181 gtacgaaatg acatattttc cgaaagggta cacggatcta agattagaag gaaagcagtg
     3241 cctttctctt catattcctt ccgatgcaga tttatctcag aataagataa gagatagtta
     3301 cgaaagtgcc attcgttttt ttgaacttta ttttcctgat taccattacc aagtgatgta
     3361 cacagatact tggttactat cacccaatct tactaaatgg ttgaaaaaag agtcaaaaat
     3421 ctgtcttttt gcagccgatt atcgattatt atcagttgat gaacaggacg atagtggagt
     3481 cccttggatc tttggacgag tagatgccca aatacatgat tatccagaaa gtacgagttt
     3541 acagcgacaa gcaaaagagc aattgcttgc aggcgagcac attggctctg gcttaggtat
     3601 tcttacttca taagtttagt acttcgaaat agtttaaaag agcgtgagac aaaagtaaag
     3661 aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc aactccttcg
     3721 gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc ttcttatttc
     3781 ttggagttaa acgcttctgt cccagcctct tttttagtgt tctatcgaac acacagtacg
     3841 ttcaataggt aaactagcta taacaataga tattaaagtt aacggacaag agggcctttt
     3901 ttaggtagct aaaacgtaat aatgaaaatt tatttccaga gataggagtc aatcagctag
     3961 tctgatcgac tgttttaaca agttgacttt cattttaact tttgatacca tttaaaacac
     4021 aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta caatcaaaga agaaagtggg
     4081 tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa agcccaaata
     4141 tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat agtgggaaaa
     4201 ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa acgtttttcg gctattttca
     4261 ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt tgattttcca tttttaacat
     4321 atgataaatt ggtagaatct ttcttttcaa attgatacca ttccctaatt ccagttatat
     4381 agttaactac agaaccacaa agcacgtacg tttataaaag tattatctgt agttattttt
     4441 caattccctt tcaacctctt attttgacca gtagtattga tcactttacc ttttaacacc
     4501 tttgttcgtt tttgaaacat ttttagtcaa gaatggaaac gtaactacat aatcaaaggt
     4561 tttgcgaaat atttcatgaa atatttcatg tagaagcgag caatacggtt gcaaataacc
     4621 attatcgttt aataagcagt tacgaaagct cccaatatct ctaacgttca gctcactcca
     4681 tttcttttct tcaaaaaaat cagtgattcg tatctctttc tatttgtcga aaaagttaac
     4741 tttaaaaagt aacgccgaaa aaatatgcaa aaaaataaaa aagcagttac tattgctaga
     4801 ataacatcaa ggaataataa gttttggttt tcagataata gtcgataagt tgtgtattgg
     4861 aattttagat acagtaataa agtatttttc atgaaatttt gtgagaatca ctattacaag
     4921 tatctttaat aaaattaatg gttaaggagg aacttaaagt agaatgtaaa tgaatagttg
     4981 tagtcagtcg attgtgatac tagactcaaa gttgcaggaa taagtttttc aatttgtgat
     5041 tggaagataa aaaatgaaaa agaagggctt ttttttatac tttgttgatt gtagtgttta
     5101 ttttccctct gtttagccaa gtaacatttt ctatgactca accagtttca agtgaggaac
     5161 atgataacct ttttcgtgct acagaagagt ttaagaaata tcaggaaaat attgttccgg
     5221 agactccatt agctagtgag ccaattactg atattgatgg aaaccaaata ggatggtata
     5281 tacagtacga agttatggtg gtaaataatg actcaatagt aaaaaataca gtaggcttag
     5341 agctaacacc gattgaaaag gtgagttcat tgttaacgta tatttatact gatgaaagtg
     5401 acgagactga gatcatttta acggattact caaaaattgc tactgataaa aagtatatat
     5461 tgtggatgta gacactagtg aaatacttta tgatatagaa attgatgaaa atttactaga
     5521 tgtagcacaa gaaatcgatc atcaaaagca ggaagctatt cgatatgctg gtagaaaagg
     5581 gaaaagaaaa tatttattac gtgaatgtag caaaagttag agaagatgaa aatgagtgga
     5641 aagaattcaa gagtcgctac tcaattaact cgactccgac atttacagtt tacagagaag
     5701 gatcgataga aaaaactgtt ttttggacta aagaaagtgg aatgtcttta gctgaagtag
     5761 aagagttttt ggattatgtt agtatgcaac agtaagggag ttcttattat gaaaaatttt
     5821 gacacatatt atgttattgt tttttcgata tctttgctgt taatattact aactttaatg
     5881 cagtataaag agtatccaga gtattttatt ttaa
//
LOCUS       DAZULO010000127         5646 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000127 DAZULO010000000
VERSION     DAZULO010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.130"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      198..364
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            512..1069
                     /locus_tag="ACG8OA_002610"
                     /pseudo
     CDS             512..1069
                     /locus_tag="ACG8OA_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1127..3166
                     /locus_tag="ACG8OA_002611"
     CDS             1127..3166
                     /locus_tag="ACG8OA_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS4977348.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3275..4426)
                     /locus_tag="ACG8OA_002612"
     CDS             complement(3275..4426)
                     /locus_tag="ACG8OA_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS4977349.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4580..5110)
                     /locus_tag="ACG8OA_002613"
     CDS             complement(4580..5110)
                     /locus_tag="ACG8OA_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS4977350.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5209..5559)
                     /locus_tag="ACG8OA_002614"
     CDS             complement(5209..5559)
                     /locus_tag="ACG8OA_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFS4977351.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         2052 a         1033 c         1050 g         1511 t
ORIGIN      
        1 tataaaaaaa cacataaaac taaaaaatta tcaaagaatc ctggtgcaac tgaaagaatt
       61 tgcaacggga ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta
      121 aatttcacta tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac
      181 tcgtaaatag aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt
      241 tgcccagaaa cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt
      301 tccaatagct aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg
      361 atgctaggtc agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac
      421 tgacttattt gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag
      481 gagatgcggt gaaaaaagat cgattggtgg aggtcgctcg gattcacttc tggttgcaca
      541 atacaaccag aaaacacaac aagcaaaact gatctctatc atgagagatt catatgtcga
      601 tattccaggt tacggaatga ataagataaa tgcagcttac tcttatgggg gagttgattt
      661 attaaatcaa acattaaagg aaaatttcaa atttgaagct ccctattatg caagtatcac
      721 atttcaagat tttatcgatt gcgttaatga actgtttcct ggtggagtca agatcgatgc
      781 agaaaaatct ctagatttag atggcgtata tataaacaaa ggagagcaag tgatggatgg
      841 taatacttta ctccagtatg ctcgatttcg ggaagacaaa gaaggagact tcgggaggat
      901 cagaaggcag cagcaagtga tcaaagctat ctctcagcag ctgaaagacg taacttcaat
      961 attcaagcta cccaaagcgg ttgggaaatt acttggtagt atccaaacga accttccaga
     1021 aagtgtgctt ctggattgtg gtatggattt cctcaaagac atatgttaga ataaacaggt
     1081 ataaatagtg aaataaagga atgacaagca agagaaggag gaaaaaatga aaagaagtga
     1141 caagcacggc aaaaatcgaa caggcgctta tattgccggc gcagtgatct taatagcagc
     1201 tgcgggtggc ggttattttt actaccagca ctatcaagaa acccaagcag tagaagctgg
     1261 agaaaagaca gttgagcaat ttgtccaagc tttgaacaag ggagattata acaaagctgc
     1321 agaaatgaca tcaaaaaagg cggcaaataa aagtgcatta tctgaaaaag agatcttaga
     1381 taaataccag aatatatacg gtgctgctga tgtcaaagga cttcagatat cgaatctaaa
     1441 agtagataaa aaagacgatt ctacttacag tttttcatat aaagcaaaga tgaatacctc
     1501 attaggtgaa ttgaaagatc tttcttataa aggaacatta gacagaaatg atggtcaaac
     1561 cacgatcaac tggcaaccca acttggtttt tccagaaatg gaaggaaatg acaaagtaag
     1621 cctgaccaca caagaagcag caagagggaa tattatagat cgaaatggtg aaccattagc
     1681 aacaacaggc aaactaaaac aattaggagt cgttccaagt aaacttggag atggaggcga
     1741 aaaaacagcc aatatcaaag cgattgcttc ctccttcgac ttaacagaag atgctatcaa
     1801 tcaggcgatt tcacaaagct gggtacaacc cgattacttt gtcccattga aaatcattga
     1861 tggagcaacg ccagaacttc cagctggagc taccattcaa gaagtagacg gcagatatta
     1921 tcctttgggt gaagcagctg ctcagctgat tggttacgtg ggagatatca cagcagaaga
     1981 tattgataag aatccagaat taagcagtaa tggaaaaatc ggtcgctcag gtttggagat
     2041 ggcttttgat aaggatcttc gtgggacaac aggtgggaaa ttaagcatta cagatgcaga
     2101 cggtgtagag aaaaaagttc tgatcgaaca tgaagttcaa aatggcaaag atatcaaatt
     2161 gacaatcgat gcgaaggcac aaaaaatagc ttttgacagt ctaggaggaa aagctgcatc
     2221 aactgttgcg acaacgccaa aaaccggtga tcttcttgcg cttgctagct ctccaagcta
     2281 tgatccaaac aaaatgacaa acgggatctc acaagaagat tacaaagctt atgaagaaaa
     2341 tcctgaacat ccattcatca gccgatttgc gacaggttat gctcctggat ctacgtttaa
     2401 aatgattaca gcagcaatcg gtctcgacaa cggcactatc gatccaaatg aagtgttgac
     2461 gatcaacggg cttaaatggc aaaaagacag ttcttgggga tcttttaaaa caacgcgtgt
     2521 cagtagtgat gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata
     2581 tgcggcacaa gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt
     2641 catttttggt gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga
     2701 agatagcttt aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct
     2761 aattactcct atccagcaag cagcaatgta ttctgttttt accaacaatg gcacacttgt
     2821 ctatcctaaa ttgattgcag ataaagagac aaaagataag aagaatgtca tcagcgaaac
     2881 agcagtacaa acgatcgtgc cagatctgag agaagttgtg caagatgtaa atggtacagc
     2941 acattctctt tctgctttag ggattccgtt ggcagcgaaa actggtacag cggaaatcaa
     3001 agaaaaacag gatgtaaagg ggcaagagaa cagtttcttg tttgctttca atcttgataa
     3061 ccaaggttat atgatggtca gcatgttgga aaataaagaa gatgatgatt cagcaaccaa
     3121 acgagcatcg gaactattgc aatacctcaa ccaaaattat caataaaaaa ttgcttttta
     3181 agtacagtaa aaggactgtg gcgttatcgt cacagtcctt ttttcaaata aacggttttc
     3241 acgatgtctc tttcacaact tctttttgat ttactcacac ttcataaact ttttttgtaa
     3301 aatatttgag aacaagtgga gaaataagtg tacttaataa aatcacgata actaaaggtg
     3361 aatagtactg tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca
     3421 tttctccacg cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg
     3481 caatctgagc accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata
     3541 tagaaataaa gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga
     3601 caaaaaatac tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt
     3661 ctttaacctt tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa
     3721 gcccaacaag atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa
     3781 tagaagaatt tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga
     3841 ctaataagaa aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag
     3901 cattgctaga agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta
     3961 aaatgtcatc taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt
     4021 ttagttcttt taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa
     4081 agaaaatcgc ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa
     4141 ccgggaagat gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa
     4201 gagataaatc actttccaga ccagctaaaa acattaataa gatcacacca atctccgaaa
     4261 agtcatggac tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac
     4321 caacaagtaa ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa
     4381 tcgttgttga aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt
     4441 tgtataaaat atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata
     4501 ctcacaaaaa aaccaacaat ccaaaagatt tttttggatt gttggttttt atacggtcta
     4561 ttttaataac taaaaatgat taacgtatag tcatttttct tattctgatg aaattgataa
     4621 tctcctggat gcccatgaaa atcaaaataa agccgaatat ttggaataga agcatcaaac
     4681 ttttgaatgg attgaacaat aagacgaatc cgccaatcaa caacagaata ctactataaa
     4741 cactccaact tttagctttt gtattcatgc tgaagaacaa ctggaacaag ctgttgatga
     4801 taacagctaa tccaagaaga atcggcagga tagaaacgat tgcaggtgca aaagcataga
     4861 cgatcagagc taagatcagg aaaaaaagac cactgaacag accgatactc caagaacctg
     4921 ttttcctttt aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa acaagatagg
     4981 cggtgatcag ataaccgata aaatgaaaaa cagcagaagg gtttagcaca atcgcaatcc
     5041 cagaaataat ataaacagca gctcttaata aggcgtattt ttgaatattt tgtttaatcg
     5101 tctcaaacat ataagaaaac ctcctttgac ttattttaac gtgaactagt cattttacca
     5161 aattaaatgt tacaaaatga gcgaaggaga agaatatctc tggaaaaatc aacttctgag
     5221 gatcttttcc atttttttgc cttttgctag ctcatcgatc attttatcta aataacgaat
     5281 ttctttcatc aagttatcct cgatgtcttc tactcgtaca ccacaaacaa cacctgtaat
     5341 caaggaacgt gaaggattgg ggacaggggc ttccgcaaaa aattgttcga aagtcacttg
     5401 tttttgtagt tgatattcta gttctgtttg ggaataaccc gtcagccaac aaatgatttc
     5461 atctacttcc tcttttatac gatcttttct ctccgctttt gctacataca taggataaac
     5521 gcttgcaaaa cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc tttctttttt
     5581 tcaaattatt tactacttat agtatataag aaaaagagaa gaaaaagcga ttattcaaaa
     5641 aaacaa
//
LOCUS       DAZULO010000128         5615 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000128 DAZULO010000000
VERSION     DAZULO010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.131"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..2009)
                     /locus_tag="ACG8OA_002615"
     CDS             complement(60..2009)
                     /locus_tag="ACG8OA_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4977352.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2051..3109)
                     /locus_tag="ACG8OA_002616"
     CDS             complement(2051..3109)
                     /locus_tag="ACG8OA_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFS4977353.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3228..3683)
                     /locus_tag="ACG8OA_002617"
     CDS             complement(3228..3683)
                     /locus_tag="ACG8OA_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977354.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4267..5070)
                     /locus_tag="ACG8OA_002618"
     CDS             complement(4267..5070)
                     /locus_tag="ACG8OA_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4977355.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1653 a         1108 c          777 g         2077 t
ORIGIN      
        1 tcttacgacc catcgtttta ttctaaggtt ttgtagtaaa tatttcttga atttcatagc
       61 tacgcccctc caatcgaaaa tacaatcttg ttttctttac tttcagcaac ggaaggcttt
      121 gcaataattt cagcattaga atcacctttt ccttcaaacg ctccggcttc actcaactta
      181 acatcgaata tttttgcgtt tggataagta attattgctt cagtaatcgc ttcttcaaac
      241 tcagattcaa ctaaatgcaa gatattcctt gcaccccctg atttagaatc ctcttgtgaa
      301 gccatgtcaa aaactaagtg catggagaga tcgtctgcat aaaaattttg accatatact
      361 ctttctttcc taggtggaag acgtaaatct atatttaaat gttttcgtac taattcagca
      421 ctcttcaaca tttcattata agcaatttcc agaagagttt gttcggataa agcccaaaat
      481 ggaactattc gtccaaatcg gttaataact tcaggcttta gaccttcacc aatcaaggct
      541 tgtttgatcc ctgatgcgac ttttcggtat tctcttttta gttcagtgct gtattcatta
      601 gaattttcct tgtttgtaac acgagatagt gagctatatc ggtttaacga atcgaacatc
      661 tccgcacctg catttgttgt cgcaaataaa attgtatttg acaatgaaac ggtgtcgtga
      721 acttctcgat catcccatcc tctttcagta aaacttatcc taccttcatc taaaattgat
      781 aaaaaggcat cgagtgtttc tacaccagct ttctcatatt catcacacac aataatcgca
      841 gaaggatcat gccttacatg agtacctata agtcgtctaa aacgcattgc atcatctttg
      901 gttttgtagt ccggcatatt aactaagaga actttatttt catcgccata aagtcctctt
      961 gccacggctt tagccattgc agttttaccg acccctgttg atcctgtaaa cagagcagtt
     1021 gcttttggtt gctttcgacc tttaaaacga tcaaatccaa gcttcatacg tttaaacaac
     1081 attgttatag tataaactgc taacggttgt ccttttatgg actttaattc ctcttgaagt
     1141 ttttccatat caaaatcaga atctagctga atattatact taccaaatat ttgcaataga
     1201 ctttctcgat tataagcatt tccgtctact cgatcttttg caatcaaatc ctccattaca
     1261 gcaattgatt tagccggttc ggacaaatcc tcacgataaa gtttgtttgc ttgtacgatt
     1321 tcttgaagta attcatcagg aatcctttgc gataatctaa aagaagcctg aggatcatct
     1381 ttaacagagg atgtatttag ccattgtctc aaaatatcga cgacaacatc gctttttagt
     1441 tcattcaatt ctaacggttg tagccgtctc cctaaggcat catctctttt gatatagttt
     1501 tcatattctt ttcttgtagt agcggtaatc atttgaagtg attcatttgc taacggttct
     1561 tttagcgaat cacccccaat tttattgcca ctcccaaaag agctaacaac tttatgaaac
     1621 tcgtcaatga agaggacaaa ttcattatct ttgtcctcag cttttaaaat tcgctcatat
     1681 tctttcaatc ttggaattaa tgtttctaaa cgtgtttgga ggacatcatc tcccccaaca
     1741 cgcaattgtc cgattgataa tttatatact tgtactttct ttccatcttg tcgctgtctt
     1801 tcagcccatt cacttgcaag tgctgtttta cctgttccgg cttcacctaa caagatagct
     1861 tgcggtgttt ccggtctatt caagatcagt gacaactcat ggagttcgtc atcacgtccc
     1921 actaacttat gttttggtgg tttcattggt gtgagagcat gattaaattc ggtttgaata
     1981 ttcttaaaaa actcggtact attttccatc tgtttgtgtt cacttcctct aaaataagaa
     2041 taatttactt ctagtcagtc aaagttaggt acttaaatgt cgtcttcatg tctccaaaag
     2101 aatctttgtt aaagtcagca tttttgtatt gatcaatcaa tttttgtgta tcattgattg
     2161 ctttaatttc ggtctccatt cgactgtcta gttgacgaat actttcttgc gaatctttat
     2221 cgtcaggatt ttgttgtaaa cgttccagat actcagcttt tgtggcttca attttcttga
     2281 tgttagtatt tgaagcattt aactgatctt ctagcttatt tacaacatta ttcttacttg
     2341 taactgctac aacttttgaa taatcaatat cgccatcttt ggtaagaaag cttccttgat
     2401 agacattagt tgaatctgaa tatggattta ttctgaaatt gatattatca ggagtattaa
     2461 caggttttgc ttttttccca aaagaaatta ctccttcatt attaacatca ttcatagaat
     2521 attgagaaag tgcttttgta actgaatctg cttcattcac attgactttg gtatcatcgt
     2581 cagctgtatc atcaccagaa attagtgata cttggtttgc aataaaaact tgataagctc
     2641 tttcctctaa tttacctttt aggaataaga aagcatcccc atcagtactt aacatcccat
     2701 agctcatttc aagcttctta ggacgatctt tttcttttgt tgccatatat aagttatagt
     2761 tttttccttg cgtagataac aactttctag cgttttggga gtaaccaagt tttactactg
     2821 ttacatctct attttgatct gtccacacat ctgaaatagt gatttctgct cctgatcgag
     2881 caaactccaa tgaagaccct ataggtgttg tattgttcac gagataataa ttatagtttc
     2941 ctaattgata aatgtagcta cccaagcctg ttaataaaaa aagcactcct agaaaagttg
     3001 caagcataat gcgtaatccg tgacgtgttc tcttatcctt atcaccaagc aaccctccaa
     3061 taatactgta ttgatctttg gtagatgttg aattgacgtt tttttccatc agtttgtctg
     3121 ccccctttca taaaagagca aacaatcaga cgttttttcg gtaattctag gttcgggatt
     3181 ttgaatcatt tcggtatatt cttccccatt aatatttaac acaaaaatta tcttcctttc
     3241 ttcgttcctt tcttttgctc tttattttcc aaataatcaa tgatttttgc tattgtacca
     3301 agatagtaat tcccctcagc tttgtttttt cggattttgc taaccattgg agtaggtaca
     3361 atctcttcat ctctcaattc actgaaagat tttttaccta gatattttcc aataacagat
     3421 tttaaaaaac gttcaaaatc tttctcgtaa gttgttttct tgccttttag ttcatggttc
     3481 atattgtcaa cgacttttgc ggtttcaata attgttttag ttctcagctt aataatttca
     3541 tctggaattt tcaatcctcg taatttcatc gttgtactaa ctggtaaccc atgctttact
     3601 gcatcagcaa tactaaaact ttttagatca tctagtaagc tctccatata gttaacaaat
     3661 tcatgccaat taatagcatt cattgtctca ccccttcatt tttttaaaat gaggttcggg
     3721 cgtaacacat ttatgttatt ataatatcat aataacataa ataaatcaat cagcaaattt
     3781 agatatccct taaagtatca gcaattattc aacaagatat ttattttagc tatttcagtc
     3841 atcataaaag actaattagc aataaaataa ctaatattat ccatttatca gacgaaaaga
     3901 aaaaacttat ctaattactc tatattaact tttaacaaat agttgacttg tccaaaaatg
     3961 ttaccttcaa tctcacaaag atattaagat gtttcctacc aaaactgata tcaccggact
     4021 ttttattctg tgttgtccaa aagtgttacc tttagtcgat caacaaatta aaaaaaattg
     4081 tttttttctt gacttgtcca aaagtgttac ccatagttgt taaaatttgt atagattagc
     4141 cattctttcg aatattattt agactgaaac ttgcttacaa aaaaacttct tgcatttatt
     4201 gtccaaaagt gttacccata cttttttaaa gaagcaggtt gtattaaaat tacaacctgc
     4261 ttcttctcaa tcttcttcta accaattatg cattgaaatg gttggcaagt ttgattctgg
     4321 ctcactaaaa gtgaatctga gccgatcaac tcttcttcct ttctttgcat agattttttc
     4381 ataatcaaaa gtttcaaata cctgtattcc gttttcatca ggagctaaaa attcttcttt
     4441 cgcctgctct agtattcttt tgtcaatatt tgtcattcta taacttgaag gtatacttaa
     4501 tgctcttcta aaatcttcaa ctttgactac ccaataacca tttcgccact ctttatttct
     4561 gtattgcatg agatgacgat acaattcttt tgtgtagcta cttcttagtt ttgtaaaatt
     4621 atcaagttca aaacgagtaa agtttttgcc aattgagttt aagataaatt caaattcctc
     4681 atttgcctta attgtaactg tttccttctg tttatctatc cggaattttt gaaaaagcca
     4741 cagctgttca tagacgtctg aatcttcaaa acggtaggtt aaagtaccta gtttgtcact
     4801 caaagctttt aacacagaat aaaaactgtc cttttctctt ctgtcgtatt tgaccaaata
     4861 acgaaagagg ttgaaatcaa aagttacttc tgctgttcct tgacgtttca tttttgataa
     4921 aatagcccaa aacacatcca tttcaacagg cttaaatcct cgcaaagctg ttgtattcag
     4981 accattgtta taacgcacaa ttgcatttgt ttgttctgat actaaatcta catcactatc
     5041 aataatcttt tcggtaactt cttgagacat tgattactct ccaatcttct ttttgcgctc
     5101 ctttaatcat gttaatctgg tactaattaa aggaaacaat tttttctgct tgttacctaa
     5161 attaaatcac aactaaaggt aacagtcaac aaagatttgt tactaatcta attttctatt
     5221 ttttcgcctt ccaaatcaag aaaaccgaag gtaacgtctt atcctagtgt gatacctcaa
     5281 atgtccactt ttgttacctt tgctgtccaa aagtgttacc cttactgtcc acttttgtta
     5341 cctttactgt ccaaaagtgt tacctttata cccctttaat acttactctc ccaagagatt
     5401 aagcctcccc taacagatat attaacagat atttaacaga tatattaaca gatatttaat
     5461 aaaggaggat tactcctctc ttatttcaaa atcttaaaaa taaaaaatct taaaaaaaac
     5521 taacactatc taggagttta ttttgcttgt gtaactttga ataattgaaa aaaaggaaaa
     5581 ggaggaagct tcagcttccc cttttattta tattt
//
LOCUS       DAZULO010000129         5531 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000129 DAZULO010000000
VERSION     DAZULO010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.133"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..985)
                     /locus_tag="ACG8OA_002619"
     CDS             complement(<1..985)
                     /locus_tag="ACG8OA_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFS4977356.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR-A"
                     /locus_tag="ACG8OA_002620"
     CDS             1137..1832
                     /gene="vanR-A"
                     /locus_tag="ACG8OA_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFS4977357.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..2964
                     /locus_tag="ACG8OA_002621"
     CDS             1810..2964
                     /locus_tag="ACG8OA_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFS4977358.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3179..4147
                     /locus_tag="ACG8OA_002622"
     CDS             3179..4147
                     /locus_tag="ACG8OA_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFS4977359.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4140..5171
                     /gene="vanA"
                     /locus_tag="ACG8OA_002623"
     CDS             4140..5171
                     /gene="vanA"
                     /locus_tag="ACG8OA_002623"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFS4977360.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5177..>5531
                     /locus_tag="ACG8OA_002624"
     CDS             5177..>5531
                     /locus_tag="ACG8OA_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFS4977361.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSR"
BASE COUNT         1669 a         1078 c         1251 g         1533 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat
     1981 gacttaaatc acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat
     2041 atctttattt atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca
     2101 aaattcgcaa aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa
     2161 gataaacaaa ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta
     2221 aaacggactc tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac
     2281 gttgttatgt acttggcgca cgatattaaa acgcccctta catccattat cggttatttg
     2341 agcctgcttg acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc
     2401 acgttggaca aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg
     2461 tataacctac aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg
     2521 cagatgaccg atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac
     2581 gcccccgagg atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac
     2641 attttgaaaa acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc
     2701 ctctccgggg atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag
     2761 ctagctgcca tatttgaaaa gttctatagg ctggacaatg ctcgttcttc cgatacgggt
     2821 ggcgcgggac ttggattggc gattgcaaaa gaaattattg ttcagcatgg agggcagatt
     2881 tacgcggaaa gcaatgataa ctatacgacg tttagggtag agcttccagc gatgccagac
     2941 ttggttgata aaaggaggtc ctaagagatg tatataattt tttaggaaaa tctcaaggtt
     3001 atctttactt tttcttagga aattaacaat ttaatattaa gaaacggctc gttcttacac
     3061 ggtagactta ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga
     3121 ttaccattgg catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat
     3181 gaataacatc ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc
     3241 tctttcgcct cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa
     3301 cgccaaatcc gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc
     3361 ctctattctt cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg
     3421 ctgcaatcat atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc
     3481 gtactcgccg gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa
     3541 cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg
     3601 caaggtactc agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt
     3661 tattgagcgg ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat
     3721 agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca
     3781 tgtgccgctc aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa
     3841 gcaaggagca tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt
     3901 taaagcatta gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga
     3961 agagtttttc tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact
     4021 tcaaagaatg cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt
     4081 gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca
     4141 tgaatagaat aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg
     4201 taaaatctgc aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca
     4261 ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa
     4321 acgacaattg ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta
     4381 aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca
     4441 agtcaggtga agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag
     4501 gctgcgatat tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga
     4561 aaaatgctgg gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg
     4621 cagctacgtt tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg
     4681 tgaaaaaagt caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg
     4741 acagcaaaat cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg
     4801 gaaacagtgc cgcgttagtt gttggcgagg tggaccaaat caggctgcag tacggaatct
     4861 ttcgtattca tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc
     4921 ccgcagacct ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata
     4981 aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca
     5041 ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta
     5101 tgatggccgc tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt
     5161 taaaggggtg ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt
     5221 tcgttgggac gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta
     5281 tgaagtaaat cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga
     5341 actggctgct acccaagggt acggattgct tctatgggac ggttaccgtc ctaagcgtgc
     5401 tgtaaactgt tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta
     5461 ttatcccaat attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag
     5521 ccatagccgc g
//
LOCUS       DAZULO010000130         5152 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000130 DAZULO010000000
VERSION     DAZULO010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.134"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..649
                     /locus_tag="ACG8OA_002625"
     CDS             <1..649
                     /locus_tag="ACG8OA_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977362.1"
                     /translation="DTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLS
                     AVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMR
                     YQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITM
                     IHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            708..1148
                     /locus_tag="ACG8OA_002626"
     CDS             708..1148
                     /locus_tag="ACG8OA_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977363.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1160..1828
                     /locus_tag="ACG8OA_002627"
     CDS             1160..1828
                     /locus_tag="ACG8OA_002627"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS4977364.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1892..2515
                     /locus_tag="ACG8OA_002628"
     CDS             1892..2515
                     /locus_tag="ACG8OA_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFS4977365.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2571..2957
                     /locus_tag="ACG8OA_002629"
     CDS             2571..2957
                     /locus_tag="ACG8OA_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFS4977366.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3170..4561
                     /locus_tag="ACG8OA_002630"
     CDS             3170..4561
                     /locus_tag="ACG8OA_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFS4977367.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1707 a          861 c         1084 g         1500 t
ORIGIN      
        1 agatactctg attgagcgat ctacatttct atatgttcaa ttgcaggaaa ataaaacatt
       61 gctgcatttt tcaccagagt tccatctaga ggggctaaca ttaggagaaa tctatcaagc
      121 agaagggctg tcggctgttt tgcacttttt taaggtagaa ttggaattac cagtaaaaga
      181 ttatatcgag ctttctcttg aatcttggga tcgagcagtg gttgaactgt ttccagaagg
      241 tttaatgatc gatacaaacg atgggaagat ccatctgact gcagcagaac tgcaaaaaca
      301 aatgcgttat caaatagatg acgaaaattc tttttctatt tttagacgtc agcaaaagat
      361 tctaaaaagc tttttaaaag tgatcagtaa aaaacggaat ctgatcaaaa cgacacagct
      421 gttgaaaaaa tatccaggtc tgcttcacac gtctattcaa tttcctcaat tgattacgat
      481 gatccatcgt tttatacgta tgcaacagct accatctaag aagctgtttc ttccattgac
      541 ggacacattt cgagtagtca acagagaaga taaaaagaaa gtgattgtga ttgactttac
      601 cagaaatcgg aatgtccttc accaagttgc aatggaaaag gccaattaag gctttttctt
      661 tttggatttt tttgtcataa taaatattag atgatggagg agataatatg atgcaaacat
      721 ggttagaaag gctaatacga tttcagcaga actatcacaa acttatgcag ggaagatatg
      781 cgaaatttga tcagttgaat cgcggtctat tgatcgctgc gcttgtcttt agtttgctaa
      841 gtcgttggtt gccctataga atcggacaat tgttgttcat cgtctttttc ggatggatga
      901 tatttcgttt cctatcaaaa aaaatctacc ccagactaaa tgagaatcaa cgttatttga
      961 aatatcttga tagagtcaaa gaaaaatatc atcagataaa aaacaaaaag caagtcaaaa
     1021 tggatcgaaa aagttatact ttttttgagt gcccgaattg tcatcaaaag cagcgtgcac
     1081 ctaaaggaaa aggacgaata cgtgtgacat gcaaaacatg tggtataaaa ttcgaaacga
     1141 atgtttaagg gagaaaagaa tgaaattagt attattacgt catggcgaaa gcgaagcaaa
     1201 ttttgaaaat tactggacag gatggctaga tgtcgcctta acagaaaaag gaaaagaaca
     1261 agcagcagtt gccggtcaaa aaatgcttga agccggactt caattcgatg ccgtttatac
     1321 ctctgttttg aaacgtgcga ttcttaccgg acaagtcgca ctagaagaaa tgggtcagtt
     1381 atggcttcct ataataaaaa catggcgtct aaatgaacgg cattatgggg atcttgtggg
     1441 taaaaacaaa gataagatga aagaaatata cggaaaagat caagtaaaaa catggcgcag
     1501 aggatatacg gaagttccgc cgttaaccgc agaaaataga tttgaccgca gatatgatca
     1561 cttggatccg catttgatcc catacggaga aagtctggaa atgacagtga atcggatcat
     1621 tcccttatgg caagatcatc tagcaccaaa actaaaagat cggcaagatt tactagttgt
     1681 gggtcatggt aatagcatcc gagcactgac aaaatattta gaagatgttc ctgaagatca
     1741 aatggatacg atcgatatcc caaatgcaca accaatccag tatacattag cagatgattt
     1801 gacaattctg gacaaaaaaa tactttaaag aacaccttta tctaaaagtg agagatagaa
     1861 aaaagaagca aaggaatgga acaaaaggaa catgaaaaat tatatatggg actttgatgg
     1921 tacattgttt gatacgtatc ccgcaatggt agatggtgca tggcaagcac taaaagattt
     1981 tggtatcagt atggacaaaa aagaaatcta cttcaaaatg aaaaagtatt ctactagtta
     2041 tttgatcaat gaatcaaact tgaatgccag agagtttaat gaattatttc accgatatga
     2101 aaaagaaagt acggaagttt cccgtccatt tccagaaacg aaacaagtac tggaaatgct
     2161 aaaagacaac ggcggtcgtc atttcatcct gacccaccgt ttgacagaat caacatgggg
     2221 cctgttaaaa gaacatcgat tggcacatct gattgaggaa gtagtaggga tcgatcagga
     2281 ctttcctcgt aaacctgatc cagcatcttt aaattatttg atcgatactt ttcatttaga
     2341 aagaacagac acgatgatga tcggcgatcg tcgtttagat atcgaagcag ggaaaaatgc
     2401 aggagtcgct acttgtcttt acgacatcga tcattttctg ggtgagatcc cggcagatta
     2461 tgtggtagga aatctgaatg aaatccttac cttgcagagc atcattaaaa agtagtatcc
     2521 cctttcaaag gagatatgtt ataattttca taaaggagtg ataaaattat atggaaatca
     2581 gaagacaaaa agaagttgta gaagcatttc attatgatat gagattgccg aaccaagaag
     2641 tagaaacaga tttgaaagta ggcttctcgc ctttaaaaga aacgatcgaa aactatccga
     2701 aagaacatag catcgtagga gcacgcttag agttccgttt agtgtttgat acatatgttt
     2761 tatctggaag tgtgagtcag atcaatcata tcctcaatcg gaaaattgaa ggtcaaaatg
     2821 atgtcacaca agaagaagtg gacgagctag tagcaccgtt attcgatatc gtacgccgaa
     2881 tgtcttatga ggtgacagag atcgccttag atcagcctgg ggtaaaattg aattttcaat
     2941 cagaaaaaca agaatagaca gtaaaaacat acctgcgata gattaaagct atcacaggta
     3001 tgttttttta ttactaattg taagcaaaaa gaatgtcatt tcgataaaaa tcgtctaaaa
     3061 tataaaagtc actgaaaaaa attttttcag gaggatggtt gaagaatcat tctcgggaac
     3121 ctttacttcc ttcgatggac ttgtcattaa taatatgagg aggagaaaaa tgacaacact
     3181 catcacagtt ttagtggttc tagtttttgc ggcattatta tatgtgttct atcgtatgca
     3241 aaagcgccat acaaaatttt caacacgcgt ctttacagca ttaggagttg gtatcgtctt
     3301 tggcggtctg cttcaattga tctttggtgt agacgacaaa gtcgttactc aatcaatgga
     3361 atggatcggg attgtaggtc aaggatatgt atcattcttg caaatgctag ttatgccttt
     3421 agtgtttatt tcgatcattg gtgcattcac aaaaatgaaa gtttctgaaa agctagggaa
     3481 aattagtgcg actgtattga cttctttact tggaacaaca gctattgcag cattgatcgg
     3541 aattggttca acaatgctgt tcggactcca aggagctaaa ttcacacaag gagctgcaga
     3601 aacagcacga atctctgaac tggcaacacg tcaggaaaca gttgaaaact tatcgattcc
     3661 acagcagatt ttatccttta tccctaaaaa cgtttttgct gatttagcag gtagtcgtgc
     3721 aacaagtacg attggagtcg ttattttcgc ggcatttgtc ggaatcgctt atctaggcgt
     3781 gaaaagaaaa gcgccaaaag aagcagcatt cttttcagat ttagtcgaca gtctgtataa
     3841 aatcattatg cggatcgtga cgcttgtttt gcgtttgaca ccttatggag tatttgcttt
     3901 gatgacaaac gttttggcaa caagtgattt tgcagctatc gtcaatttag gtaaatttgt
     3961 cgttgcttct tacgtagcat tgcttgctgt tttgattgtc cactcactga ttttgattgg
     4021 tgcaaaagtc aatccaatca actatttcaa aaaagccttt ccggtattga gctttgcgtt
     4081 tacttcacgt tcaagtgctg gagctttgcc attgaatatc gagactcaga caaaagcttt
     4141 aggagtagat gatgcaacag ctaatttctc agcaagtttt ggtttgtcta ttggacaaaa
     4201 cggctgtgct ggcgtttatc cagctatgtt ggcaacgatc gtcgcaccag cagctggagt
     4261 gaatgtcttt agtttggaat ttattttgat gcttgtggca attgtaacaa tcagttcttt
     4321 tggtgtagct ggagtcggtg ggggcgcaac atttgcttca ctgatcgttt taggggcaat
     4381 gaacttgcca gtagcaatcg ttgggttagt tatctctgtt gaaccattaa tcgatatggc
     4441 acgtacagca gtaaacgtca gcggtagcat ggtagctggt atcgtgacaa gtgcacgcat
     4501 caaagattta gatcgcgatg tcatcaatga tgattctaaa gtgatcgatg tgaatgcata
     4561 gtcaaaaaga ggttgtgata gaagtggtca gctttaagaa ataagaagaa gtttcctaaa
     4621 aatagtttct caaatttttg tgaaatttcg gcttatttcc gagaagctac ttctgaaaca
     4681 ccgtgaataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     4741 aaatagttcc tcatatttta cgtttggttg agcttattac cgcagatgca agcttttgaa
     4801 caccgtttaa gaaagaggtc gtgagcctgc cgtttcactc tgagtcacaa agagcaattt
     4861 caaaaaatag cttctcatgt ttgttgaata ttgtgtcttg tgaccgagga gttgtcagga
     4921 gaacaccgtt taccagaggt tgtgacttgt ttttagtcac gacctctttt tttatttatt
     4981 ccatataaat acacattatt tattccgaaa agtgaaagcg caaataacaa aaacaaccaa
     5041 cttatttatt attaaagaaa agcgtattta agtaggacga ttctgttgag agctttcgta
     5101 gattttccca ttttcctatg gtaaactgac aaagttgaga atgttagaac ag
//
LOCUS       DAZULO010000131         5061 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000131 DAZULO010000000
VERSION     DAZULO010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.135"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(205..660)
                     /locus_tag="ACG8OA_002631"
     CDS             complement(205..660)
                     /locus_tag="ACG8OA_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LMxysn_1693 family intestinal colonization
                     protein"
                     /protein_id="HFS4977368.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDD
                     EYYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIAR
                     NTSASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
     gene            complement(1063..1236)
                     /locus_tag="ACG8OA_002632"
     CDS             complement(1063..1236)
                     /locus_tag="ACG8OA_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977369.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYN
                     SPNEELFVKETDK"
     gene            complement(1917..2456)
                     /locus_tag="ACG8OA_002633"
     CDS             complement(1917..2456)
                     /locus_tag="ACG8OA_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS4977370.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
     gene            complement(2472..3395)
                     /locus_tag="ACG8OA_002634"
     CDS             complement(2472..3395)
                     /locus_tag="ACG8OA_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4977371.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            complement(4523..4678)
                     /locus_tag="ACG8OA_002635"
     CDS             complement(4523..4678)
                     /locus_tag="ACG8OA_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977372.1"
                     /translation="MKLTKLTISLVIIAGIILYFVLPANQFFLYFGIPTILIVNGVRI
                     FEQRKKM"
     gene            complement(4697..>5061)
                     /locus_tag="ACG8OA_002636"
     CDS             complement(4697..>5061)
                     /locus_tag="ACG8OA_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFS4977373.1"
                     /translation="FSVLFLINILNTSFSEGINPNEVNGSLLLFFLNAATYGAFLEEG
                     IFRFCMIDPQANKKQQYISILISSFLFSIVHGGGLSIFFIGIILSFVYIQTKNIWYSI
                     VAHGFYNTIGILIYLISI"
BASE COUNT         1607 a          839 c          771 g         1844 t
ORIGIN      
        1 aaaaaaaact atatttaaag agttttttta gatttacttc tttgaattca cgaatgaagg
       61 tagaatgaaa tagtataatt attagcacta aaaaaaatat atcactatgt tcaaataaaa
      121 aatatatatt tgacggtact cctaatgatg ggatagcaaa ggtcaaaacc aagtaagcaa
      181 tactaattat ccattttatt gtcattaggc aggaatataa tcaatatatt gtgtatttgc
      241 tgtagaagtt tgagtacgaa tatatttatt atctacctta cgataaactc tgtaggttac
      301 agttattctt gcattttgac gatatttttt aaattttcca cttgcagaag tgtttctggc
      361 tatttcgatt ttcatcccaa atacatttgc actgacagat aactttgtac ctcgactatg
      421 agtttctata cctaaataag tacggcttcc tttagcaatt gaccagcttt gaggtttaga
      481 ccaagtcctt gtttgaataa ctggttcgtc taacacatag tagtactcat catcagaata
      541 agtggctata tcagaaatgt tgttctcatt agcgaatacc gctccgctag gagtaatagc
      601 agaaccaaaa gttaaaatag aagtagataa taaaaaggtt ttaaataact gttttttcat
      661 caaaatcacc tcgaatataa gatacttaag aaaacgcttt ctttatataa ttagaataat
      721 caggaaattt aatgttgtca agagggttta ttttataaaa gctgttatat taaaggttag
      781 tagacataat gtacagtatg ccaagtagcc cttgtatcaa ccgatacagg ggctcacttt
      841 gtgattttta attggtctag gtaaaaagct ctttttaagg cttgattttc tgcagttgct
      901 tctttccgtt tctttgtgaa tttttaccag tcatttttta ctggattgtt atttttcata
      961 aaatatgata aatttttggt agcgctttat aagcgcgttg ctttttcaag aataaacact
     1021 cattattttt taactggctg ctcggaagag cagtcttttt tattatttat ccgtttcttt
     1081 tacaaacaat tcttcgttag gactgttgta ataatttgaa ttcaatttaa gttctctgtt
     1141 ggaattgttt tttccaatta cggataatag catggggcta aaacctaata ttcctagaga
     1201 aatagaaccc agtaccaaca cagttttttt taacataggt tatacctcca ctttttttaa
     1261 cgctaccact tgtatacaca ttttaaatac tagccttcag tttatcaaat gtttaaattg
     1321 aacggatata aatcaaaatt aaattttcaa aagttgttct aaaaaatttt tcttttttgg
     1381 aagttactct aacttttttt gcttgtggtg cttggtatgt tttctccgcc tcccaggttc
     1441 ttgaccattt tctcttataa gtgggatttt taccacgttg agagaaaatg gtcaagaacc
     1501 tgggaggcgg agaatgccct taataccaag caccgcaagc ctttcccata ctttattcga
     1561 tgatcccacg atgatctcat aatgattcta caatgaccct acgatgatca aaattctcaa
     1621 aaaaaataaa cgaaatttca aacgattttt ttgaaatttt gttaaaatca catttgtttt
     1681 tttatgactt tttcaacaaa atcgattaga atttattttg tttttccacc aaaaattgag
     1741 tgagatttca acctatttta agcaataagt caccatatag tttgatagtt tacaactata
     1801 aacattgatt gtttacagtt gtaaactatt ttttctttct cataaaaatt tgagtgatct
     1861 ccccatcagg tagtacaact ttttcctcta ctatccgaac taacttctag aaaattttag
     1921 agattggttc tattagattt ccaaatttgc caccactttt tttcaataag tggttgtgta
     1981 ttgtctgctt ttttttcaga aaaggcagtt gattcggttg ttggagtttc ttctgtataa
     2041 gttaaatgga gctgttcttg taggttctca atttgttggt tcgcttgtag agtgagttgt
     2101 tgttgctggt cgagtaattt ttgcatttgt tttatttgat tatctttttc ttgtagctgt
     2161 atatcttttc gatttaactg tttttctagg aaagaaagga gttttaattc ggaaacttgg
     2221 ttggtgtttt tatggttttt ggtttttgat ttttcgccaa ctagttttcg atttaagttt
     2281 gcgttatcga ctaaaaacat tgattttata gctttttgac cggtttcgtt aatcacaaac
     2341 ttgtttccgt tttttgcaaa ccaaagtttt cgatttttgt cagaaacttt tttgttaatt
     2401 gctgttttac taactcctaa ttcttccgca agttctttga ttgttttatc tgccataaat
     2461 actccttaag cttaatcgaa taagttttgt aaatttccta gtaaatcttg tttgagtttg
     2521 tttgaaagtt ctcgtttttc tgcctgtttt tcttcagttt gttgttgttg tttaaaatca
     2581 gcttcatgag tgcctaaggg aagatttagt attcgatctt tagcgcgcca tttttcttct
     2641 cgtgttaatt ctccattgtg atcaatatta aataatttta tacgtaaatc ttcttgtttc
     2701 cctttggaaa aatcgtcagc atggttaatt tcagctttcc aagtaaaacg ataaccgata
     2761 acaggtttac ctcgaccttt accatatttt ttacgaatag ttaatccttt aaataaggga
     2821 gtgagttctt ctttaattgg ccttaagata aatttgtcaa tatttgacgg actattccaa
     2881 taacttttag gaatatctag taaatcaaaa aatttttctt tggaaaattc agtccagcct
     2941 tgagttcgat attgttttaa taatcgaaac atagtttttg aatatgtgct atccaaatta
     3001 gtaaattgct ctagattaaa tcttgtccag ttctttaatt gattaaaaag tcctttaaat
     3061 tctggattaa ctgaaattat aacaagttgt ttatctcgta taatgctata ttttgtgaaa
     3121 gcaacaaatt tagttaaagt atttccatca tcattccaag cattaatact taataatttg
     3181 tcatacgtgc tactaagatc ttttataaat tgttccccac gttctgtata accgcttagt
     3241 tttttcaaat cgtgaaatga aaaaataatc tcatctacac ctttttcacg tacacgtgaa
     3301 gcaattgaaa aaaataagtt catttcacga ctattaaatt ttctcagagg aatatgattt
     3361 aatctatttt catattttac aatttcattt gccataagct tctcctactt ttataaagta
     3421 tgaaataaaa gtaacatact ttataaaaat actcaataat gtcggaaaac ctacttgaaa
     3481 agcgtagaaa acctacttga aaagcgtaga aaacctactt gaaaagcgta gaaaacctac
     3541 ttgaaaaaac tctgaattgc ttgggagagt aaggacacag ccgacataaa agagatataa
     3601 aaagagatat aaaaagagat ataaaaagag gggaggctaa ttttcccgcc taaaaaatca
     3661 ctcctttttt ctctaaacag gttcgcgaat ttatcaaaac tgccaattcc aaaccttgct
     3721 ccctacggtt cgctaaaaag gtgtgatcgc tctgctctca cattatactg cgcagtcgcc
     3781 tccggctcct ttgctaaaag ccgttcataa ggaacccttt gagaagctct gagcgttcgt
     3841 ttaagacatt ttaagacagt cctagtataa atcccttatc ttgttcttaa aacgccttac
     3901 aggcggtcag ggcaagccct aacaacccta aaattgtcta aatgccctct aaaattcgat
     3961 ttaagcactc ttaaacctat tcttatatat ttatatactt aggcgtttca aacgtcttaa
     4021 aacgcaaatt agagcttgat aaagatattt gctcgtgtgc ctagactttt aaaaaagtcg
     4081 gcacacgcca tgacaaagct gaaataaact gcttcagctt tgtcatggcg gattcgctca
     4141 cttgaaaaag tgtttatttc gctcgattat tagcatatat tttttagctt ttttgctagc
     4201 caaattttgc attaatttgc tcataagtcc caccctttca gtacgtccta cggacgtatc
     4261 gttcgtaatg tgaaagtacc tttaaaggta ctttctacaa aactcactaa aagggtttgg
     4321 caagctctat atttgctagc caaataccta cgtatttggt gcaaataacc gcttgtcaaa
     4381 ggggcgcaag ctcccccttg aatcccccct caaaacccac gctccacaat acaaaaaagg
     4441 ctagcaaatg ctagccactt tttgtaaagt ttcgcttgtt tcaccttcgg tgaaattgtt
     4501 gctttgcaac gatcaggtag agctacattt tttttcgttg ttcaaatatt cttacgccat
     4561 ttactattaa aatcgtaggt attccaaagt aaagaaaaaa ttgattagca ggtaatacaa
     4621 aatataaaat aataccagca ataattacca aacttatagt aagttttgtt aatttcatga
     4681 agtatgccac cttatcttat atagatatca aatagattaa tatgccgatt gtattataaa
     4741 atccatgagc aactatggaa taccatatat tttttgtttg tatataaaca aaacttaaaa
     4801 taataccaat aaagaaaata cttaggcctc ccccatgaac aatagaaaac agaaaagaag
     4861 aaattagtat tgaaatatat tgttgttttt tgtttgcttg aggatcaatc atacaaaatc
     4921 gaaaaattcc ttcttctaaa aaagctccat aagtagcagc atttaaaaag aataaaagga
     4981 gagaaccatt tacttcatta ggatttatac cttctgaaaa agatgtattt agaatattga
     5041 ttaggaataa aacagagaat g
//
LOCUS       DAZULO010000132         5024 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000132 DAZULO010000000
VERSION     DAZULO010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.136"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG8OA_002637"
     CDS             complement(<1..182)
                     /locus_tag="ACG8OA_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4977374.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACG8OA_002638"
     CDS             517..951
                     /locus_tag="ACG8OA_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4977375.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACG8OA_002639"
     CDS             974..1294
                     /locus_tag="ACG8OA_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977376.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACG8OA_002640"
     CDS             1435..1626
                     /locus_tag="ACG8OA_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977377.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACG8OA_002641"
     CDS             complement(1697..1954)
                     /locus_tag="ACG8OA_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFS4977378.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACG8OA_002642"
     CDS             2112..2375
                     /locus_tag="ACG8OA_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977379.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACG8OA_002643"
     CDS             2399..2587
                     /locus_tag="ACG8OA_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977380.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACG8OA_002644"
     CDS             complement(2670..2855)
                     /locus_tag="ACG8OA_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977381.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACG8OA_002645"
     CDS             3070..3441
                     /locus_tag="ACG8OA_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4977382.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACG8OA_002646"
     tRNA            complement(3591..3663)
                     /locus_tag="ACG8OA_002646"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACG8OA_002647"
     CDS             complement(3709..4137)
                     /locus_tag="ACG8OA_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977383.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACG8OA_002648"
     CDS             complement(4197..4955)
                     /locus_tag="ACG8OA_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFS4977384.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1750 a          784 c          983 g         1507 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tatt
//
LOCUS       DAZULO010000133         4906 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000133 DAZULO010000000
VERSION     DAZULO010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.137"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            317..1240
                     /gene="truB"
                     /locus_tag="ACG8OA_002649"
     CDS             317..1240
                     /gene="truB"
                     /locus_tag="ACG8OA_002649"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFS4977385.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1244..2185
                     /gene="ribF"
                     /locus_tag="ACG8OA_002650"
     CDS             1244..2185
                     /gene="ribF"
                     /locus_tag="ACG8OA_002650"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFS4977386.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2537..3322
                     /locus_tag="ACG8OA_002651"
     CDS             2537..3322
                     /locus_tag="ACG8OA_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4977387.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3335..4186
                     /locus_tag="ACG8OA_002652"
     CDS             3335..4186
                     /locus_tag="ACG8OA_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS4977388.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4310..4762)
                     /locus_tag="ACG8OA_002653"
     CDS             complement(4310..4762)
                     /locus_tag="ACG8OA_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4977389.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         1636 a          801 c         1051 g         1418 t
ORIGIN      
        1 tgaaaaaata aataaaaaaa acggaaaaaa gactggaaga atggccagtc ttttttttcg
       61 tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc caatcaaatg
      121 gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaagcataac atgatttcta
      181 tttacgtgag catgaatttt gttacgtttt tattgccgaa aaataaaaac cgttcattat
      241 gatgtcaaat tcgtcagtaa ttgatgcaat gtgttataat aattcagtta aatcaaaaat
      301 gaaatggaga aaagacatgg acgggttatt acctttatgg aaagaacgcg gaatgacaag
      361 tcatgattgc gtattcaaat taagaaaaat cttacatacg aaaaaaatcg gacatggggg
      421 tacgcttgac ccagacgtag acggtgtgtt gcccatttgt attggaaaag ccacaaaagt
      481 tatcgaatat ttgacagatt ctgggaaaat ctatgtaggc gagataactc taggtttcag
      541 tacaacgacg gaagatcgat ccggagaacg ggtagaaaca aaaccagtag atatgtcttt
      601 atctgaaaaa aaaatagatg aagtgatggc ttcatttatt ggtgagatca cccagatacc
      661 accaatgtac tcagcggtaa aagtcaacgg taagcggtta tatgaatatg cccgcaataa
      721 tgaaacagtg gagcgtcctg ttagaaaggc tcagatttac cgttttatcc ggacaagtga
      781 attgacaaga gacgaagtga caggaaccat ttcttggcgc ttcgaagtag aatgcgggaa
      841 aggaacctat gtccgcacat tagctgtaga tacaggcaag aaacttggtt accctgcgca
      901 catgtctgat ctgacccgaa cagcaagtgc cggtatgtcc cagaaggatg ctctgacatt
      961 agaagaagta gctcaagctg tgaaaaatgg gacaatcgat gattgtttgc tgccaataga
     1021 aaaaggagtg gaacggttca aacgagtaga tattactgat gaagtatggc agaaagtaaa
     1081 aaacggtatg agacttgatt atactgtatt tgcgctccca gaaatgccgt cagaagaaat
     1141 tgctttgttt tataaaggaa aagttgtcag tatttatcag ccaaacccga aagaaaaaaa
     1201 caaattaaaa cctagtaaag tattgagaaa tgaggaataa cgaatgaata tcatcaagat
     1261 cagacatcct taccaatcag aacaaattcc agaagacgat gtcgttttag tgctgggatt
     1321 tttcgacggc gtgcatatcg gacaccagaa agtcataaag acaggtatgg aaattgcaca
     1381 aaaagaagga ttgaaattag cattgatgac tttcaatcag catccatcta ttgtctttaa
     1441 aaagatcgat cctagcagcg tgaaatattt gactagcctt aagcaaaaag aagaaaaaat
     1501 ggccgctttg ggtatagatt atttatatga agttgatttt acttcttctt ttgctcattt
     1561 agcaccgcag gaatttgttg atcaatacat cgtagggtta catgcaaaat atgctgtttc
     1621 cggttttgat tatacgtatg gaccaaaaga cattgcagat gtcgctcatt ttcctgaata
     1681 tgccaaagga agattcaaaa tcgtcacagt ttctaaagaa gaagaacaag gtgaaaaaat
     1741 cagctctacc cgtattcgtg gattactaga cagcggacaa gtaaaagaag cagcaaaact
     1801 attaggttgc acatatgagg tagaaggggt agttgtccat ggcgatgcac gaggcagact
     1861 actaggattc cctacagcaa atgtcaaagt aaagagcacg gttcatttgc ctaaagaagg
     1921 tgtttatgtg tcggaaataa aaattgggga aacatggtat ccagccatgg gttcaattgg
     1981 acataatgat acattcggtg aaggacggga gctgactgta gagttgtata ttttggactt
     2041 taatcaagat atctatggtg aacatgtcgc ggtacgctgg aatcaatttt taagagatca
     2101 agtgaaattt tctagtgtag aggaattgat tgaacaactc aaggaggatg aacaagcaac
     2161 agctgaatat ttttctcagc aataaaacat tatgcataaa atctcttgtt tatgtataac
     2221 tagcaattac aataaaatgg gtgataaata agcccaggaa aggggctgtg acaaaagtct
     2281 tgtcacagcc tttttttcca aataaatggt ttttcagaag tagcttcttc ggaaataagc
     2341 cgaaatttct tgaagctgac cacttctgtc acaacctctt tctttataga aataaaagga
     2401 cactagcaat tcatgaaaac gattgctaga gcgtatcatt tgctttagac tatatatctt
     2461 ggtgtacact cactttaagg cggaaataaa agttatccgt aataacaggt ttaggtatga
     2521 aaaaggagtg ggtactatgt acaaggaatt agaaaacaaa gtcgcagtag taactggtgg
     2581 ctcaaagggg attggaacag cgattgctca acgattcggg aaagaaaaaa tgaatgtagt
     2641 tgtcaactat aactcggata aagaaggagc agaaaaagca gtcaaagcaa tcgaagaggc
     2701 aggcggaaaa gcagtctctg tacaagcaga tgtcagttct gaagaaggaa tacagcgttt
     2761 acttgatacg gctttagaaa attttgggac actggacgtt tgggttaata atgctgggat
     2821 ggaaaatcag catccaaccc atgaacttag ccttgaagat tgggaaagag tgttaaaagt
     2881 caatttgact ggcgttttta tcggaacgaa agcggcttta aattacttta cagagaataa
     2941 taaaaaagga aatatcatca atatgtcttc tgttcatgaa cagatcccat ggccaacgtt
     3001 tgctcattac gctgcttcaa aaggcggggt aaaattattt accgaaacgg tagcaatgga
     3061 gtatgcaccg aagggaatca gaatcaatag tataggccct ggtgctatcc gtacacctat
     3121 taatgcggca aaatttgatg atcctgaaca aaaagaactc ttggaaagta tgatcccact
     3181 aggacgcgta ggtgaaccaa aagaaatagc ggcgacagct gcatggttgg cttcagacga
     3241 atccagttat gttacaggta ttacactatt tgcagatggt gggatgactt tatatccttc
     3301 tttccaaggt ggaaaaggat aagggtgaat aaaaatgggt ttattgattg cattgatccc
     3361 ggcagttggc tgggggatcc agccactgat tttgaaaaag attggtggca gaccaacaaa
     3421 tgagatatta ggaactggta ttggagcggt gcttgtcggc ttactcgttc aattattcgc
     3481 ttctccagct ggtatttcag tgactacatt tttgattagt ttactttctg gtgcattttg
     3541 ggttattgga caaacaggac agtatacatc tttcaacttg attggtgttt ctaaaacgat
     3601 gccaatttcg actgcattac agttagtcgg aacctcgttg attggtgtct tcgcttttgg
     3661 agaatggtct ggaagtatgg ggaaaatcat tggagcagca gcaatcattc ttcttgtatt
     3721 tggttctgct ttaacggcaa tcagtgatga tcctggaaaa aaaggcggta tcacaaaagg
     3781 aattagtcta ttagcatcaa caagtattgg ttattgggta tacagtgcat tgcctaaact
     3841 agttgatgca agcggacttt ccattttctt tccgcaaatg atcggtgtct ttttaggggc
     3901 agtgatttat gtgattttcc gtaaaccaac cgcattttct gaagacaaga gttggaaagc
     3961 aacgatcgtt ggagccatct ttagtgtgtc agcattggct tatatttttt cagcaaatga
     4021 aaatggggtt gctactgcat atatcattac acagctaaac gtagttatct ctacattagg
     4081 cggtatgtac atcctgcatg aaaataagac accaaaagaa aaaaaattaa cgattatcgg
     4141 attattgctg atcgtagtag gaagcatggt cacggtcttt atttgaggat aaagatggta
     4201 aaatttcatt tattaaaaaa atatttgttt gaaccaaaca gtaagtactt ataaaaaaat
     4261 agcgctagaa tttccaacat ctctggaatt tctagcgctt tttaattgtt taagatacaa
     4321 gggaaatatc tgtctggatc aataattttt cattgatcca ttgactaaag tttacgacat
     4381 aattgaagtt accaccgatt agtatctccg catgattgtc tttataaaga tcgtattgta
     4441 aaagacggtc ttttttattt gcgtaaacat cgttatggta ctggactaat tgttctcgga
     4501 attgattgat tgctgtaaaa tcacgatatt gaaggttgaa acgcatcgtg attggtgcat
     4561 ttagcatgaa tcgctgcatt tcttttgctg cttcctctgc ttgatctccg tataggaact
     4621 caaaaagatt acgttcagaa aatttccatt gcccgccaac ctttgttcct tttagttttc
     4681 cttcatgaag ataatttctg atagaacgtg tagtaacacc taattgttcg gctaattcat
     4741 ttactgagta aaaatctttc aatgttcacc ctcctatgtg aatggttcta ctgacttagt
     4801 tgatatttgc ttaatcacga aatgtagaaa gaacgatttt aattaaggtc attgtaaaaa
     4861 tcaagtcaga aaattctaac ttttatttct ctaaagaaat tcgttc
//
LOCUS       DAZULO010000134         4804 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000134 DAZULO010000000
VERSION     DAZULO010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.138"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..290)
                     /locus_tag="ACG8OA_002654"
     CDS             complement(<1..290)
                     /locus_tag="ACG8OA_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS4977390.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
     gene            complement(283..624)
                     /locus_tag="ACG8OA_002655"
     CDS             complement(283..624)
                     /locus_tag="ACG8OA_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977391.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(798..1523)
                     /locus_tag="ACG8OA_002656"
     CDS             complement(798..1523)
                     /locus_tag="ACG8OA_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFS4977392.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            1576..2052
                     /locus_tag="ACG8OA_002657"
     CDS             1576..2052
                     /locus_tag="ACG8OA_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977393.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            complement(2058..2240)
                     /locus_tag="ACG8OA_002658"
     CDS             complement(2058..2240)
                     /locus_tag="ACG8OA_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977394.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(2240..2539)
                     /locus_tag="ACG8OA_002659"
     CDS             complement(2240..2539)
                     /locus_tag="ACG8OA_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977395.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            complement(2644..2847)
                     /locus_tag="ACG8OA_002660"
     CDS             complement(2644..2847)
                     /locus_tag="ACG8OA_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4977396.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(2862..3608)
                     /locus_tag="ACG8OA_002661"
     CDS             complement(2862..3608)
                     /locus_tag="ACG8OA_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFS4977397.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            3686..3994
                     /locus_tag="ACG8OA_002662"
     CDS             3686..3994
                     /locus_tag="ACG8OA_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977398.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            complement(3965..4222)
                     /locus_tag="ACG8OA_002663"
     CDS             complement(3965..4222)
                     /locus_tag="ACG8OA_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HFS4977399.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(4520..4660)
                     /locus_tag="ACG8OA_002664"
     CDS             complement(4520..4660)
                     /locus_tag="ACG8OA_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977400.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
BASE COUNT         1321 a          935 c          753 g         1795 t
ORIGIN      
        1 tctgtttccg tatgtccttt aaccgttact gagacagata cataagtccc tgtttcatcc
       61 ataagaaaag gtttatattc ctcaacaaga aagtcttgat gagttccaga aacaaccctg
      121 tagtgtttat actcattaat agttaccgtt gcctgtggat cattcttttt cataatctcc
      181 cacgcgtgag cccaagataa ataatcaaaa tttccttttt tcttgagaat tttatttaac
      241 ttacgactaa aaagtttttc aaagttcgtt gtccctttga tttcactcat caaattctgc
      301 ctccatttca gcaatgtatt tcttacctga tccgtaataa gagatatcaa tcaagttatc
      361 tctgttgtac tcttctagcg catcaatcaa gccatcttcg atgacataga tgtattcagg
      421 tttatttgaa tgttttgata gatggataag gtaaacatga tcccaaatac tcacaaaatt
      481 tcccaagtca tcttgatcac atgatagttc ttcgttcgtc aaaagatttc gtctgatttt
      541 tcgattattt gtttccttga tattcgactt gccccattca gaatcagtca aatattgatc
      601 taaagtggaa agttctttat tcatatgcta aaattctcct tagatatgtt ttgtgactct
      661 atgcttgccg gctgagtcac ttttttattt gttgccatgc tttttgcttt tcgatatgtt
      721 gttggcttag gatgtttggt ttattgtgtc tccaccagcg attagcaatt accgtcccta
      781 ttcttagcgc ttcagctcta ttcattttca tcaccgaaaa gtctttgttg tctgttcagt
      841 tgatcgattt ccatgcggat cgcagtttct ggtaaccaca tttcaataaa tgaaacagca
      901 tcatcgaatc tcttacgagg taactcgcca tatcttggga ttgaaaaggt acgtttaaat
      961 tcagaccaaa attttgagaa tacttttttg ctgatttctt cataagctcg gctttctttt
     1021 ccccctagaa cttccataac tttcatattt cctttttgct taatttcaaa ctcttgttgt
     1081 ccgctaattc gcatagtatc tttaagcatg gaaacatctt ttttaacatc tttcatttct
     1141 tctaattgat agatcatcat gtcttcaatt gtttgaggaa cagtattctt ccgaataaca
     1201 tcttccattt cgttgaaagc ttcaataaat tttagtttga aatttattgc tttacttcca
     1261 gtgaatccca tagctagcaa ggaaaatccg tctctattca tgaaataaac tctccgactt
     1321 cttccgtatg agtctggctc gttcccttcc acaaacatct gtccaaaatt ggacccatct
     1381 tcaacattgg ccgaattttc gaccgatctt tttattgatt caattgcttc tagtacatgc
     1441 ttatgtttct ttttgaaact ttctgccact tgtaagctcg tagttacagc ttctttattt
     1501 ttcaaaatta ctaattcttg cattatttct tctctccttt ttggtataat tttcgttaga
     1561 aagtgaggtg attctatgag gaaaaagctc aattacattc ttactgcgac tttaaccata
     1621 tatataattc tgtttattac cttatctttc ctgtcttcaa ttaaaaaaat tccttttatt
     1681 acattgaata aaaatagtat tttgaaggag ttcaataaca ctgactacat cgcatttcta
     1741 aaactttttt cagtctgtgt gctaatatca tgtgtaatta tttttattgt tgctaaaaca
     1801 tgttggacaa tgttaaataa aaaccaacgc gatgttgaaa atattgctat atttttaatt
     1861 aagattactt cctcaatatc aattatcatt ccgatgactg taactttttc aaaagaacag
     1921 tttaatgtag cggctactta tctttcattt atagcgctat tttcttttgt tcttcctaaa
     1981 agaaataaga gcaactttga caacaaggag ctggatgaag tacgcaatag cagcccaaag
     2041 gaacaaaaat aaaaaaatca tattttagtc agtccctccc gactggcttt tttcgctctg
     2101 tgctcagctt catcaagccc cataaaaatc caaaccatgt aaacgattgt ccctatcaac
     2161 gcttgtctgc ttccccagag gcctaaagcg tagatgatta gcggtgcgct gaatactagt
     2221 gctctgttga atttgcccat taacttgcct ccttcaaaac atctgaaggc gataaacttt
     2281 ttacacgata tttgtttgcg tctttccact ttaaaaacca aataaaagta tggtaatgaa
     2341 taaatgtggt tgagtgacct ggacgaagta ttccttctgc aaattcagga attgtttcca
     2401 tttccttgca gtattcaact aaagtagttt ttgacatgcc gtggaacttc tccaagatta
     2461 aactttgtcg gtaccagtcg tctggtttta ctgccttctc agcagcttct atcagctctg
     2521 aaagtgttgg ttttttcact tattatccct cctaaaaatt atcgttccat actcgatgtt
     2581 taacatttct tgaagctctg attttgtaag aatgatttta tcttccaacg actttacctc
     2641 cctctacaat tcgtacatag tgataatcga atctataatt ctgtttgctt ctgcagaagt
     2701 ctttttaccg tttaaaatta aagataagta gcttttgcta attccaaatc tttcagcaag
     2761 catggtgtaa gttaagaact ttgaactttc gacatattct ttgatttttt ctctatctcg
     2821 ttgagtgatt tctgcaatgt cagtcatact aaaactcctt tctaaccaat ttcctctaaa
     2881 tccatttgag ggtaatagcc ctctgcgatt aataagttgt aaataaacac tcgccctttc
     2941 tgtgtccatt tggtattcat tacaacttta gtgccgccat cggctttcgg tatctcactt
     3001 gtgtgcgatt ttgtgtatcc ttggttcata tgttttcggt aaagtatcca ttgtccgcta
     3061 actttatgct gaacacctaa ctcgtttagc aatttgttta gagcaattgc cgacattccg
     3121 taatcagctg caatttgaga agtagctacc gtatctgtcg atgaaagaat cgtatctaag
     3181 tagctaatct ttggttcgta ctcggcaatt tgttgttcga gtaattggtt cttttcttct
     3241 aaatccgctg ctaaacgcaa tgcttgtgca aaactttgag gtacgtttga atagctacct
     3301 gtttttctaa ttgttggaag gacttcactc gttacccaac gtttaaattt tttggcagag
     3361 ggaagttttg attttaagat taaactgtaa aggcctgact cgttaatcaa aacaactgct
     3421 gttccattga cggtgaacga atcgttctgc gtcttatcct ccaagtccac atgatctcga
     3481 atagcttttt gcggattcga atatcctaga acgtctgcta catcttttcc tacaaaatat
     3541 ggttcatcat ttactaaaat agttcgaact tcgttttgct cgaaattaaa aatttgtggt
     3601 gtattcatat ttctcattcc tttctttggt ataatttcct tatcagtgtg acaggctgaa
     3661 ataattgtta aggaggtgaa aatttatgaa accagaattt gtaattacta tgcttgatgg
     3721 agaaaaaatc aaaatatttg ccgatacttt ggtcgttgga tacgatgatg cgtcaaatat
     3781 cgaagaaaaa gatggaatgt tctattcttc tcaaatttac gtgaatacct tgcaaggtga
     3841 tattggggta tctactcttg caacatcaag accgcttgta ggtcttatcg gctttttgtc
     3901 aaaagcaaca ttcttttctc tcggcgatga tatagattcg gatgaaactg tcatctataa
     3961 aacatcagct gttaaaagta tcggtttcaa atagtaattc ttgctcgttg atagcggaag
     4021 ccaacttttc ttccaacaca tcggcgagct ttctttttgt tggattcatt tcaaagttga
     4081 tagtcttgag aagcattttt gcatccttaa ctttttgtcc ttgcaattcc ttgatgattt
     4141 tatcggcttg gctatcaatt ccatgtgtta gatcatctat cattttttgt accttgattg
     4201 tttggctcat ttccttgttc attgtttagc ctcctttaat aaatgatttt ttgtattttt
     4261 taatttccat aaattcctca aattgctctt tggtattatt accacgacca tacatattgt
     4321 gaaattcttt atggcaactg acacaaagtg taacgccgtt tgaaatcgct gcggatttag
     4381 ttgttgcatg gctaaatcac gaatcacaag tatctactaa aggcgaatca atttcgccaa
     4441 aagaaattgt tcaagcttat ttagatattc attatgctgt tatctatggt cagcttccag
     4501 aagaccgtaa aaacgatgac taaacgtgag ctaaaatctc tgctatggct gcaaccatgg
     4561 cagagtcttc tttatctacc gcgtgttcca tcgccacttt tgcttgtttc agtatgtcta
     4621 atttcaaatc ttcgattttt gccgagactg ttttttccat attcttctct cctttctttt
     4681 atattcgtaa acaaatttaa caacttttta aaaaactgcg ttgactttat tagattaata
     4741 atctatacta aatgcatagt taaataagac atataaactt tgatttaaaa agctttcttg
     4801 gcgg
//
LOCUS       DAZULO010000135         4788 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000135 DAZULO010000000
VERSION     DAZULO010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.139"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            564..989
                     /gene="bacA"
                     /locus_tag="ACG8OA_002665"
     CDS             564..989
                     /gene="bacA"
                     /locus_tag="ACG8OA_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFS4977401.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1019..1183
                     /locus_tag="ACG8OA_002666"
     CDS             1019..1183
                     /locus_tag="ACG8OA_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977402.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2141..3118
                     /locus_tag="ACG8OA_002667"
     CDS             2141..3118
                     /locus_tag="ACG8OA_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4977403.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="ACG8OA_002668"
     CDS             3128..3625
                     /locus_tag="ACG8OA_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS4977404.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="ACG8OA_002669"
     CDS             4001..4282
                     /locus_tag="ACG8OA_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977405.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="ACG8OA_002670"
     CDS             4284..4700
                     /locus_tag="ACG8OA_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977406.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1758 a          817 c          785 g         1428 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtacacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DAZULO010000136         4756 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000136 DAZULO010000000
VERSION     DAZULO010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.140"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..1118
                     /locus_tag="ACG8OA_002671"
     CDS             72..1118
                     /locus_tag="ACG8OA_002671"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS4977407.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            1135..2358
                     /locus_tag="ACG8OA_002672"
     CDS             1135..2358
                     /locus_tag="ACG8OA_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS4977408.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            2447..3334
                     /locus_tag="ACG8OA_002673"
     CDS             2447..3334
                     /locus_tag="ACG8OA_002673"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4977409.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            3420..3650
                     /locus_tag="ACG8OA_002674"
     CDS             3420..3650
                     /locus_tag="ACG8OA_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFS4977410.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(3707..4672)
                     /locus_tag="ACG8OA_002675"
     CDS             complement(3707..4672)
                     /locus_tag="ACG8OA_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS4977411.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         1606 a          790 c          968 g         1392 t
ORIGIN      
        1 aaaaataggg cttaagtttg agtgacagtt tgtaggctat gttatactat ataagtgaag
       61 ggggacgtaa ggtgaaggta ttattatatt ttgaaagtga aaagatgctt gcaaaatctg
      121 gaatcgggcg tgcgcttgat catcaaaaac gtgctctgac ggaagtcggt atctcttata
      181 cactagatga aaaagaagat tatgacatcc tgcacatcaa tacatacgga atcaatagtc
      241 ataatatggt caataaagcc agaagagaag gaaaaaaagt tgtctatcat gcgcattcta
      301 cagaggaaga tttccgaaat tcctttatcg gatcgaacca gctttctcct atcgtaaaaa
      361 aatatctggt aggattgtac caaaaagcgg actatctgat cacgccaaca ccttattcaa
      421 aacagttgtt ggaaagctat ggtatccgtg tgccgattca agctatttcc aatgggatcg
      481 acttagagaa atatcaccct gatccaatga aagaacaaaa gttcagagaa tatttcaaac
      541 tgtctccgga tcaaaaagta atcatttgtg tcggtttgtt tttcgaacgt aaaggaatca
      601 ttgatttcgt agaaattgct aagaagatgc ccgaatacac atttatttgg tttggtcatg
      661 ttcccatgta ctcgattcct cggaacatcc ggaaaattgt aaaagaagat catcctgaaa
      721 atgtcctgtt tcctggttac attcgtggag agatcataga aggtgcgtat tcaggtgcag
      781 atctattctt tttcccttct tatgaagaga cagaagggat cgttgtttta gaagctttag
      841 caagcaaaca aaacgtactt gttcgagata ttccagtgta taatggctgg ttagaagata
      901 gcaagaactg ctatatgggg aagacaaacg aagaatttat ccgactgatc caacagatta
      961 ccaatcaaga actaccgagt acgacacaag ccggttatca aacagcaaaa gagcgaagta
     1021 tcaataaaat cggtgaagaa cttaaaaatg tatacgaaag tgtactgaat gaaaaagtct
     1081 ctagcaaatt aaaaaaagta aatcaggtca aggactagtt tggaggcgaa aaacatgaaa
     1141 atcggctttt ttaccgatac ctactttccg caagtcagcg gagttgctac atcgatcaaa
     1201 acattaaaac aagaattaga aagacatggg catgaagtct atatttttac aacgaccgat
     1261 cctaatgcgg ataagttgga aaaagatgtg attcgaatgc caagtgtacc atttatttct
     1321 ttcaaggatc gaagagttgt tgttagaggc atgtggtatg cttatttggt tgccaaagaa
     1381 ctagaactag atcttattca tacccataca gaattcggag cagggatact tgggaagatg
     1441 gtagctaaga agttgaaaat acctctgatc catacttacc atacgatgta tgaagactac
     1501 cttcattata tcggtaaagg aaaagtgatc aggcaatccc atgtaaaata cttgtctaga
     1561 ttatttgcaa atcatacgac aggtgtagtt tgtccaagtg aacgtgttat tgatacatta
     1621 cgcagctatg gtgtaatagc accgcttcgc gtgattccta ctggtatcga cattgaaaaa
     1681 ttcaaacgtt ctgatatcac tcagcaggat atcagcaaat taagggagtc actaggtctt
     1741 caggaaaacc atttgatgat tctttcttta agcaggattt cttatgagaa aaatatccaa
     1801 gccttgatta atgggtttcc tcaggtcatc tccgcttatc ctaacgccag attagttatc
     1861 gtaggaaaag gaccttatgt agaagagtta gaggaattga tctcagaact gcagttagaa
     1921 gaatttgtgc agttcacagg agaagtggac aacaatgatg tagcacttta ttataaagcg
     1981 gcagattatt ttgtcagtgc ttctacttca gaaacacaag ggcttactta tacagaagcg
     2041 atggcagccg gaacaccttg tgtcgtggaa ggaaatgctt atttgaaccg attgttcgat
     2101 catccttcat tagggaaaac ttttgaaacc gacgaagact ttgcaccagc acttattgat
     2161 tatatttcca gtgaagttcc taaagatcca aaagttttag aagctaaact ttatgatatt
     2221 tcatctacac atttcggcga agctatgatc gaattttatc aagacatggt tgtttatcat
     2281 gaagaagaga tgcgaagaaa agaagaagaa gactcgattg aacgaatcaa aataaaatta
     2341 aattcattta aaagataaaa ttcaaacatt ttttatgaaa aaatccttgt ttctagcgta
     2401 tactagcata gaagcaagga tttttgattt ttaggaggtc gaaaaaatga aacttggttt
     2461 tatcggaaca ggcgtcatgg gaagtgctgt cgcacgccat ctactagaag caggacacga
     2521 agtagctgtc tataatcgga caaaagcaaa agctgatcct ttagtaacag aaggagctat
     2581 ctgggcagat acaccaaaag cggtagcaga acaaagtaat attctcttta caatggtagg
     2641 ttaccccaaa gatgtagaag aaatctacta tggacagtcg gggatttttt ctgcagatat
     2701 ctcaggacat attttagttg atttgactac aagtacacca agcctggcag aaaaaattgc
     2761 aaaaacagca aaagaaaaag gcgcagatgc gttagatgca ccggtatccg gtggtgactt
     2821 aggagcaaag aatggtacac tgacgatcat ggtcggtgga gaagaagccg tttatgatca
     2881 agtactgcct ttattcaaag aatttggtac gacatttaca ttgcatggtt ctgctgggaa
     2941 aggtcagcat acaaaaatgg ctaatcagat catgattgca ggtacgatga caggaatgac
     3001 ggaaatgctt gtctatgcac aaaaaaacgg acttgattta aagaaagtga ttgagacgct
     3061 ttcaggtgga agtgcagcta actggtcttt aagcaattat tctcctcgta ttttaaaaga
     3121 agattacaca cctggttttt ttgtcaaaca ttttatcaaa gatttaaaga ttgcattaga
     3181 agaagcagaa aaaatggatc ttgtattgcc agcaactacc caagcattga aactatatga
     3241 agagttggca gataaaggtt ttgagaatga cggaacccaa gctttgatca aactttggtg
     3301 gcctgaagga aaaataccag aaaaaaaatc ataaaaaacg agttttttta gagaaaatca
     3361 atgcaagtag gctatgcttt tgatacaata taacagaaat aagaaaagga ggacctccga
     3421 tgacacattc acaattagtc gcaatcataa aacgactgga ggctatggtc gagtcagcag
     3481 ataatgaact gcaagttcgc cgttttgaaa aagaaggcgt agagaagtgt acagtaactt
     3541 atgataaagc gaccgataca tttgaattaa ctgaaacaga tactcatcag caatacgaat
     3601 ttgataatat cgatatcgta gcgatggaaa tctatgactt aatccaataa attttaggat
     3661 aattgacatt tgaagttgaa gatgcctaaa agaagccgag aagcaactaa tttagttgct
     3721 tttcagcttc ttttttgttt aacagttcaa tttcaaactg ttctttttct aattttttgc
     3781 ggtaactgat caagatttgt acaggatcga tttcttcttt tgacaattga cgtttcaaag
     3841 ataaatgagc ttctttcttt tcataataga agtcaggaga aacgtcacca gaggtaatca
     3901 ttgtttctgc aagttgacca taacaaacga ggtagtgctt tttctgactc tcatctgctg
     3961 ctaatggcat gctgaaggtg agcgatggtg ttcttaaaaa ctgcttttgt tcctggatcc
     4021 gtctaataaa ataagtaact aattcgatat ctttttgaag cataccggct ttttcgaaat
     4081 tcaactgata gatgaaatgt tgttgtcttt ttttcaagta agtcagtagc tttttatttc
     4141 ggccttgaaa cagcccttgg agttcctttt ttttctgttc aaaagtcatt tcctgtaggt
     4201 caggaagctg gactcgcaaa gctaacaaac taatttcaga caaccaaggc atttgataag
     4261 tttcttccat tatacgcaaa atagacggca tttttttata gagacgaaaa ggcccgtatg
     4321 ttcgagccgt aggagtatct gttaatacaa atcccttatt ggtaatatgt acataattat
     4381 aattactgaa ataattcatt tgtcggttat aaaaaggacg gtaatgttgg atcaattgac
     4441 actcgagcaa taaagcatct aattccgtgt ctactacgac gaaatcaaaa tctgttatat
     4501 gatgaatcat acgtaatact ttttttgaat gctgtttatt ctgatgaaaa tagctgctta
     4561 ctctattttt gagccgtttt gctttaccaa catagatgac cttaccatct cggtctttca
     4621 taagatagac acccggcagc atggggagag ttttcatttt ttcttttaac ataggatctc
     4681 cttttacgct ttgaataaaa agactataac aaattttgaa ataatggaca agttttaggg
     4741 aattctatag ttctgt
//
LOCUS       DAZULO010000137         4713 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000137 DAZULO010000000
VERSION     DAZULO010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.141"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            108..359
                     /locus_tag="ACG8OA_002676"
                     /pseudo
     CDS             108..359
                     /locus_tag="ACG8OA_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            452..1246
                     /locus_tag="ACG8OA_002677"
                     /note="aph(3')-IIIa"
     CDS             452..1246
                     /locus_tag="ACG8OA_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFS4977412.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1656..1739
                     /locus_tag="ACG8OA_002678"
                     /pseudo
     CDS             1656..1739
                     /locus_tag="ACG8OA_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            1788..1871
                     /locus_tag="ACG8OA_002679"
     CDS             1788..1871
                     /locus_tag="ACG8OA_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFS4977413.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1996..2733
                     /gene="erm(B)"
                     /locus_tag="ACG8OA_002680"
     CDS             1996..2733
                     /gene="erm(B)"
                     /locus_tag="ACG8OA_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFS4977414.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2996..3859)
                     /locus_tag="ACG8OA_002681"
     CDS             complement(2996..3859)
                     /locus_tag="ACG8OA_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS4977415.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3861..4133)
                     /locus_tag="ACG8OA_002682"
     CDS             complement(3861..4133)
                     /locus_tag="ACG8OA_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS4977416.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4150..4365)
                     /locus_tag="ACG8OA_002683"
     CDS             complement(4150..4365)
                     /locus_tag="ACG8OA_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFS4977417.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4457..>4713)
                     /locus_tag="ACG8OA_002684"
     CDS             complement(4457..>4713)
                     /locus_tag="ACG8OA_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HFS4977418.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1447 a          796 c          865 g         1605 t
ORIGIN      
        1 atttcttggg agttttgaga ctactatatg cgtttttttg ttgatttacc aacatgttta
       61 gacattttcc tctaggaaaa aattaactag caccacgggt tatcttttat atagaagata
      121 tcgccgtatg taaggatttc agggggcaag gcataggcag cgcgcttatc aatatatcta
      181 tagaatgggc aaagcataaa aacttgcatg gactaatgct tgaaacccag gacaataacc
      241 ttatagcttg taaattctat cataattgtg gtttcaaaat cggctccgtc gatactatgt
      301 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
      361 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
      421 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
      481 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
      541 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
      601 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
      661 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
      721 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
      781 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
      841 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
      901 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
      961 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
     1021 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
     1081 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
     1141 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     1201 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagatttaga
     1261 tgtctaaaaa gctttaacta caagcttttt agacatctaa tcttttctga agtacatccg
     1321 caactgtcca tactctgatg ttttatatct tttctaaaag ttcgctagat aggggtcccg
     1381 agcgcctacg aggaatttgt atcgataaga aatagattta aaaatttcgc tgttattttg
     1441 tacatttaac ttgacggtga catctctcta ttgtgagtta ttagtggtac agttttcaac
     1501 cgttttaatt ataaaaaagt ggtgcatttt taaattggca caaacaggta acggttattg
     1561 caggtgtatt tcttatctat gggtttaaca tggattttat cattaaaatc atgagtattg
     1621 tccgagagtg attggtcttg cgtatggtta accctaaagt tatggaaata agacttagaa
     1681 gcaaacttaa gagtgtgttg atagtgcatt atcttaaaat tttgtataat aggaattgaa
     1741 gttaaattag atgctaaaaa tttgtaatta agaaggaggg attcgtcatg ttggtattcc
     1801 aaatgcgtta tgtagataaa acatctactg ttttgaaaca gactaaaaac agtgattacg
     1861 cagataaata aatacgttag attaattcct accagtgact aatcttatga ctttttaaac
     1921 agataactaa aattacaaac aaatcgttta acttctgtat ttgtttatag atgtaatcac
     1981 ttcaggagtg ataacatgaa caaaaatata aaatattctc aaaacttttt aacgagtgaa
     2041 aaagtactca accaaataat aaaacaattg aatttaaaag aaaccgatac cgtttacgaa
     2101 attggaacag gtaaagggca tttaacgacg aaactggcta aaataagtaa acaggtaacg
     2161 tctattgaat tagacagtca tctattcaac ttatcgtcag aaaaattaaa actgaatact
     2221 cgtgtcactt taattcacca agatattcta cagtttcaat tccctaacaa acagaggtat
     2281 aaaattgttg ggaatattcc ttaccattta agcacacaaa ttattaaaaa agtggttttt
     2341 gaaagccgtg cgtctgacat ctatctgatt gttgaagaag gattctacaa gcgtaccttg
     2401 gatattcacc gaacactagg gttgctcttg cacactcaag tctcgattca gcaattgctt
     2461 aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt aataaaactt
     2521 acccgccata ccacagatgt tccagataaa tattggaagc tatatacgta ctttgtttca
     2581 aaatgggtca atcgagaata tcgtcaactg tttactaaaa atcagtttca tcaagcaatg
     2641 aaacacgcca aagtaaacaa tttaagtacc gttacttatg agcaagtatt gtctattttt
     2701 aatagttatc tattatttaa cgggaggaaa taattctatg agtcgctttt gtaaatttgg
     2761 aaagttacac gttactaaag ggaatgtaga taaattatta ggtatagggc acctctaata
     2821 actaccaatt aattcacctc aaaatgaaat tagaaaaaaa cagcgaaatt tttaaatcta
     2881 tttcttatcg atacaaattc ctcgtaggcg ctcgggaccc caacatgagg aaatgttttt
     2941 ataaaaaaag agtagttacc gttttttggt aactactctt ttcctttttt aaggtttaaa
     3001 tacctggaag tttaggtgtt ttgggtattg gtggtgtagg tggctgcaag ctttccaatt
     3061 tttgttgaat tgctttaaat tcaggtgtct cttggtgttt atttagaacc attttttgct
     3121 ctattcgttc taaagtcggt tgaatttctt tccctgatac tttacgattc aattcttttt
     3181 ctaaggtttc ttttggacta atagaaggtg tttccaaact tgaatagagt tttactcctt
     3241 ctctgttata tagccttata tcgctaaata agcccgtttt atgaagggtt tctaaattgg
     3301 tcggtaagtt tttgacaaca atatcatgcg cttgttttgg tgttgccctg gctgtcattg
     3361 gatcatctgc atacatggtt tcatatcgtt caattgttcc taaatatgag ttgattttag
     3421 gtactgccat gacatacatt tttgtttcat aacctttggc ttgaagcatt gttgcggttt
     3481 gaataggaac gtctgttgtt cgtcctgtac cttcgatcac caaattatac ccttgatcgc
     3541 tcaaacggct tatgatcgct tctgtcatgc gattagaata aggggtaacg tgttttacta
     3601 cgtctttttc ataaagtttc actagttcat caaaattagg gtgctgttgt ttaaaggtat
     3661 cattatcaat gacaataaca ttcccttgtg tttcttcaaa aattgctgat cgcaaactgg
     3721 ttttccctga ccctggttgc ccaccaagta aaaaagcggt tggcgattca accgcttttt
     3781 ttccttgaat caattcttct aaattatcat ttaagcgatt ctcaaattgt ttgtcagtaa
     3841 aattgactat atttgccata ttaagccact ttctctttat tcaaaacttg ttttgagtaa
     3901 cgattcattg accgccaata ctcatgaacg gcttgtaatt cttctaaaga ataatcaaaa
     3961 agatttacac gttttgcaag ctttaattgg tttaataaat tgacttccaa taattgagag
     4021 tcatttttat ttaattcatg gttcaaaaca tagtttaata ctcgattata aacgcttgcc
     4081 gataattcgt taatgatctc tatttcaaat gttttttcat acgtaactgc catttcattt
     4141 tcactccttt taaagtttgt caggtaaata tttccgtata tattcttcta aggcttgatc
     4201 cataacttct tttacgtttc cgccattctt tgctgtttcg atttttatga tatggtgcaa
     4261 gtcagcacga acacgaaccg tcttatctcc cattatatct ttttttgcac tgattggtgt
     4321 atcatttcgt tttgcttttt gtgcgcctaa atttcccaca atcactcact tctttctatt
     4381 tcttcttact cttattttat catcaacaat cacaaatcac aagtgatttg tgattaatca
     4441 cttgtgattt gtgattctat tctttttcgt tttctaattg aataattcgc tcaagcattt
     4501 caaaaaatac gttcttatac atggataaaa cttttttgtc atagccttta tgctctgtaa
     4561 tgccgttttt agaccaggta cttactttat tacttcgctt gataatattt tggaaaacca
     4621 agttatcttc tttatgttgc ttatacagtt cttccaggtt tgattttatc gttgcgctgt
     4681 ccgtatcaac taaataagga acaaaaccga tca
//
LOCUS       DAZULO010000138         4682 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000138 DAZULO010000000
VERSION     DAZULO010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.143"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(231..842)
                     /locus_tag="ACG8OA_002685"
     CDS             complement(231..842)
                     /locus_tag="ACG8OA_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4977419.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(965..1090)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1172..1888)
                     /locus_tag="ACG8OA_002686"
     CDS             complement(1172..1888)
                     /locus_tag="ACG8OA_002686"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS4977420.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1894..2463)
                     /gene="scpB"
                     /locus_tag="ACG8OA_002687"
     CDS             complement(1894..2463)
                     /gene="scpB"
                     /locus_tag="ACG8OA_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFS4977421.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2447..3217)
                     /locus_tag="ACG8OA_002688"
     CDS             complement(2447..3217)
                     /locus_tag="ACG8OA_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFS4977422.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3214..3585)
                     /locus_tag="ACG8OA_002689"
     CDS             complement(3214..3585)
                     /locus_tag="ACG8OA_002689"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4977423.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3604..4491)
                     /gene="xerD"
                     /locus_tag="ACG8OA_002690"
     CDS             complement(3604..4491)
                     /gene="xerD"
                     /locus_tag="ACG8OA_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFS4977424.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         1286 a         1006 c          805 g         1585 t
ORIGIN      
        1 tcagtttttt aaagctttct tctttttcat tgtagcgttt cctttttttt ataaacaaac
       61 attttcgtct tttaaaagga gaagaatttc acatttgcaa agaattgatt gatttttatg
      121 cataaatcaa aaaaatactc aaaacaaaca aataaaacgt ttacattatt gcatgtcttg
      181 tatttttttc caaaaaaaaa gaccaagaaa tttcttgatc tttttgatat ttatttgact
      241 gctgtatgac gttgttcgaa ctgatgttgt tttcgactta accatggaag aagttgcgca
      301 tgcagcacta aaaagacagc actcacgatg aatcctttga tcaagttgaa aggaacgatc
      361 ccaatcagta catagtttgc taaagacatg cccagcatct gtgttgcgtt cagccctaaa
      421 aacattaagt aaagaggcgt aataacgaaa taatttgcga tactcataaa aatagttaga
      481 gcgatggttc ctgtaatgac acctaaaaat ttgtttcttc ctttatgctg tttctttaca
      541 ctaaagaaat aataaatcgg gaatgtaaag atcgttgttg ataagaaact agcagtgtct
      601 cctaccaaat tatccggaga aaaacctgta gtcagcagat gtaatactga acgcaagaac
      661 gctgttgcaa ctccggccgc tggaccgaat aagaacatgc tgatcaatac cgagatatca
      721 ctaaaatcga tcttcaaaaa actaaaagcc ggcatgatcg gaaaagcaaa atactgtaaa
      781 actaatgcga tcgcagcaaa catcgctact gcaaccattt tctgtacacg ggtgttcttc
      841 atttgaaatc ctcctgttag gactcctctt cagcgaactt tttgacgttt ctctccaaat
      901 gaaaaaactc tattttccag ggaaaataga gttagagtta tttgatcaag cgtcgtcaaa
      961 taagctctat cttctttatc cagactatac tgtcggtatc ggaatttcac cgattcagct
     1021 atttttttgc aaaataggtc gtggactata accaccggtc gggaatttca ccctgcccct
     1081 gaagacgaac cttatataat ttttgtttac atattcatta tacatatcta ttcaaaaaac
     1141 gcaacatcta acaattataa aaagtaagtg ttcattttga gtggttcagc aaactgctga
     1201 cttctttttt gttcagttca cggtaatccc caggacgtag gccttgtaaa gtcagatctc
     1261 cgtatttttc acgttttagt ttttgcacag gaagaccaac tgcttgaagc atatttttca
     1321 cttgatgatt acgtccttcg tggatcgtca attccacgat actgtggttt gtcttaggat
     1381 ccactgataa aatttggaat ttagctggag atgtttttcg tccttcgatc tttattcctt
     1441 ttgtcaaagg tgcaagcatc gattttgtag cgatcccttt tacctttgca acgtaaactt
     1501 tatccacttc atgttttgga tgtgtcaggc gctgagcaaa atctccatca tttgtcagta
     1561 gcaagatccc cgaagtatcg taatccagtc gacctacagg atagatacgt tcttttacca
     1621 tcggcaaaag atccgtaaca acttttcttc ctttgtcatc tgcaacagca gaaataactc
     1681 ctttaggttt gtacaataaa tagtaaccgt attcttcttg ataaattggg acttcatcca
     1741 cttccacagt gtcatgtttc ccaactttta tccctaattc ccttacaact tgtccgttta
     1801 cttttacacg tcctgcaagg atgaattctt ctgcttttcg acgagaagtg atgccagcgt
     1861 gggcaattac tttctgcaat ctctccatta caattattct cctttattca ttccatcaaa
     1921 gaatagatct ggtgacacgt cttcttcaag tgaaatttcc atttcttgtg tatcaggcag
     1981 ttcttctaat gatttcaaac caaaataatc taagaagtag ccagttgttc catataatat
     2041 cggccgacct ggtccttcta ctctaccttt ttcttcaatc agtttatggg cagctagacg
     2101 ctgtaccgct cctgaaacct gaactcctcg aatctgctcg atttcagctc ttgtgattgg
     2161 ctgtttataa gcgatgattg ctaaggtttc gacggcggct tgagataatg tgtttgatgt
     2221 cccttgcgca tatcgcttaa gtaagggagc cagctctttt ttagtcgtta atacataatg
     2281 gctttccgtt tcaaaaatat gcaaagctga gtgcgagttt tgagtgtaag attcttttaa
     2341 ttctttcact aaacttgtga cttctgcttc gctgagattt aacagtccgc ttaattcttc
     2401 attactgatc ccttcctcgc caacaataaa aagaagggct tcaagttcac ttaatttcgt
     2461 catgttcggt atctcctact gctggataaa gcataattga ttcatagttt tctttttgtt
     2521 gcgcaatgat ctgccctttt ttcatcaatt ctagcaaagc cataaaagta gtgacaactt
     2581 cacttttaga aggtgactca aaaaaagaat cgaaagtaca gccatgcgca tttcttttta
     2641 tacgctcttc gataaatacc attcgttctt cgatcgttgt gtcatcgggc gtgatcgttt
     2701 tttccacttt ttgagaaaat ttcttacgtt ctaagatttt ctgcatggca cggatcaaat
     2761 cgattttttt gacttgtcct cgcttcaacg gcttttcagc atccgctaac ccatcgatgt
     2821 tcataggttc ttttgtgtaa taaagacttc gttcctctgc tttttctgtc agtctttcag
     2881 ctgcatattt atatttacgg tattctaaaa gctgagtgac caattcttct cgaggatctt
     2941 cttcccataa ttcttcatcg tctgtgccga attcttggac aggaagcaat gtcttgcttt
     3001 tgattgccat caatgtagca gccatcacta agtattctcc tgcaacagct agctcgagtg
     3061 ttttcattgt atggatataa ttcatatatt gctccgtgat cgtagcgatc ggtatatcat
     3121 atatatcgat ttccatttgc tgaatcagat gaagcagcag atcgagagga ccctcgaata
     3181 catctaattt aatattgatc tccgtcaact cattcactct ttcttctttt tctttgctag
     3241 ccattttcca atgtaaggct ctgtgatttc agttgcacgg atggagacat ccgggtactt
     3301 ttcctgtaat tcatctaaca tttgaaagcc catattctca ccgcgataag acggattcaa
     3361 agaaatgtca tggatgatca gctgatcgtt cgttgcccag taaataccaa taatcccttg
     3421 aatgttggtt gaaccttctg gcgtaaagaa aaaaagttca taatcgtctt cagattcgta
     3481 cgtatcaatc tccttcaaca aagaagtttt acctgtcagt ttcttatgga aactcaatag
     3541 tcccatagca attttacggt tttctttccg gtaatgtgtt aacatcttct caccttccta
     3601 tgactaagcc cttggaaaat gctgcttata aacatccgtc attcttttct tcgtgatatg
     3661 tgtataaatc tgagtcgtag agatatccgc gtgacctaac aattcctgta ctgtccgcaa
     3721 atcagctcca ttttctagca gatgtgtggc aaagctatgc cgcagcgtat gtggtgttac
     3781 ttctttgtaa atcccagcct ctcgtactaa ctgcttcaga tttttccaga ttccttgccg
     3841 agaaagacct ttcccatgat tattgacaaa tacatgcatt tcagatgcgt cctttacaag
     3901 taatggacgt gcttcgtcta agtagcgttc cagccattgg atcgcataat cacctaaagg
     3961 aatgatccgc tctttatctc cttttccaac tgtctgtacc agtccaagag aaagatgaag
     4021 atctcccaat ttcaatccaa tcaattcact gacgcgcatc ccagtagcat acatgacttc
     4081 caagattgca cgatctctta tccctaacgt ttttgtcgta tcaggtgctt caatcagctt
     4141 ttctacttct gtcagagtca atgtacttgg taatttctgt gctttcttcg gcgtatcgat
     4201 atgttgcatc ggatcatgat cagtgatacg ctcttgccgt aaaaactgat ggaatcttct
     4261 gagacttgag atcatccgag taacagttgc tgtcgctttt ttttcttcat gcaacttctc
     4321 cagaaaacgg atgactaaaa agcgatcgac atcctgccat gaagcaattt tctgctcctg
     4381 tagaaaatgc aagtactgtt ccaaatcacg ttcatagctt tgtcgtgtat tggcggacaa
     4441 tccccgttca atattcaagt agtggagata atcgttgatt tgctctttca taataatccc
     4501 cctcatgcct ctctatcata tcagataccg cttcattctg atagtctttt tccataatct
     4561 gagcaaagaa aaagccgtct agacactcag acggactggt ttcttttttc ggctctataa
     4621 tttatgggac tgatgaatca gaattgattc ttcagcccca ttttcatttt aggtattaaa
     4681 aa
//
LOCUS       DAZULO010000139         4646 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000139 DAZULO010000000
VERSION     DAZULO010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.144"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..717)
                     /locus_tag="ACG8OA_002691"
     CDS             complement(133..717)
                     /locus_tag="ACG8OA_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4977425.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(928..1131)
                     /locus_tag="ACG8OA_002692"
     CDS             complement(928..1131)
                     /locus_tag="ACG8OA_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977426.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1141..1563)
                     /locus_tag="ACG8OA_002693"
     CDS             complement(1141..1563)
                     /locus_tag="ACG8OA_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977427.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1801..2415
                     /locus_tag="ACG8OA_002694"
     CDS             1801..2415
                     /locus_tag="ACG8OA_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4977428.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2580..3404)
                     /locus_tag="ACG8OA_002695"
     CDS             complement(2580..3404)
                     /locus_tag="ACG8OA_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977429.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(3428..3583)
                     /locus_tag="ACG8OA_002696"
     CDS             complement(3428..3583)
                     /locus_tag="ACG8OA_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977430.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
     gene            complement(4042..4299)
                     /locus_tag="ACG8OA_002697"
     CDS             complement(4042..4299)
                     /locus_tag="ACG8OA_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS4977431.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(4292..4561)
                     /locus_tag="ACG8OA_002698"
     CDS             complement(4292..4561)
                     /locus_tag="ACG8OA_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4977432.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT         1533 a          816 c          626 g         1671 t
ORIGIN      
        1 taatattaac aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact
       61 tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat tttcagcatt
      121 aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt ttgccaaata
      181 acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa taggtgtacc
      241 gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt caggacgacc
      301 accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt ccaggatcaa
      361 attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc ctgtagaact
      421 tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac gctcattcag
      481 tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta agcgccaaac
      541 gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg gtcgattact
      601 ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat ttaggctgtc
      661 ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg gcgtatccta ttttcaaaaa
      721 aatcactcct tcattaattt tattgtaaca taactttatc catatagttt aactgaacat
      781 agatattgtt atgggtttat gaacatgaaa tgaattgttt ttttgaaaaa taaagcaaaa
      841 aaagaaagtg tccagaaatg ggtgatttta tgaacacttt caattagaaa aaaagaatgt
      901 cgactagaca ctcttttttt aaaatcatta tttttctttt tgatatgttt tttggtctct
      961 tttttttctt ttttcttcag aatcttctag attagaaatt gatttttgaa ttttttgtgt
     1021 ctcctgtaaa gttcgctgaa cttgtaaagc tgattcttct ttcggtaaat taattttact
     1081 tagaatatcg ctatcttgat aaaaatttgg tgtgttcaca aattctggca taaaaaatcc
     1141 ctactttcta gttaaggttc ctgaataagt cgcaatccaa ttgtttccag ctgatttata
     1201 actttgtaaa tacaattttc catagtacca ataaccattt cttttttctt tccaattccg
     1261 tgattgagga ggtctagaag gccaaccaac tttatcaaaa atcatcgttc ctgtcactcg
     1321 tatcgtttta gaagctgcta gagcaagtaa tggagaaact attaaataat tttcagtaac
     1381 agttaagtcg ttagttgggt taccaatggc tattccgtcc ctatctaaag gagttaattc
     1441 ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg taggaattgt
     1501 agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa acttattttt
     1561 cattgtctat cacctcgaaa tcaaaactaa gaaaacgttt tcttaataaa tcttaacaga
     1621 taaatcaagt tatatactag aaaattcata aaatatattt ttaatgtcat aatcgtttaa
     1681 attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacata
     1741 tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta ggtaaaagtt
     1801 ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg ccagattgaa
     1861 gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg cgcaacgatt
     1921 actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga tatttttatt
     1981 gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc agtcaattac
     2041 ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc tgaagcaatt
     2101 ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt ggccatgatc
     2161 gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa gatcgcaaaa
     2221 gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa agatccccaa
     2281 cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc gattgctaag
     2341 attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa agacagcatg
     2401 gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta atttcctaca
     2461 tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata aaattccaac
     2521 aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac ttatttacgt
     2581 taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc cttattatct
     2641 tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact aattcctata
     2701 gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat ttgagattta
     2761 tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata cataaagcta
     2821 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     2881 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     2941 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     3001 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     3061 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     3121 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     3181 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     3241 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     3301 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     3361 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     3421 aaatcagcta atttttcttt agaataaaaa aaacatttat atcagaatca ataaattcta
     3481 ttttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
     3541 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
     3601 ataaaaatta cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta
     3661 aattctatga gaagtttatt tttgttgttt ttgctaatta actaaaacta gtacaaaaaa
     3721 tttaaaatag attattaaac ctattgctta ttctattcag tcatatatat aagcgtcatc
     3781 ctttagtaac taacatccca cactacaata gtattataac atatctattc aacttcaaat
     3841 tgttgaaatt taagccattt atatttactt ttcctcaact ttagaaatga ttttactaac
     3901 cttatatccg taatacgcgt aacaaacaca ttcattaata acaatcaaca ctaagctata
     3961 ttagcttttt aaatgactag ggtaaataaa ataaggatca aaaactttta agtttctgac
     4021 cctttcctta cttccgattg gttaatagtg atcttttgca gaataaataa atatcgtttc
     4081 attttcaact ctatatatca gtctatgttc atctgtaatt cttctggacc attttccaga
     4141 taaatcatgc tttaatggct caggttttcc taatccagca aagggggaac gatcgatatc
     4201 ttttattaac ttgttaatct tttttatatt gcttttgttt ccttgctcat gccaataaag
     4261 ataatcatcc caagcatcat cagaccaagc cttaatcatc agattcaacc tcgattaagt
     4321 catgtgtttt aaatgcacct ttggagaatt gttcatctcc tcgacgaatt ttttccatga
     4381 cgtaattatt agaaagtgtt ctcaacgttt cttgcataga atcataatct ctttttgata
     4441 atacaacaac tgtatcttct acatctttac ttgttacaat aagtgtttca gcatcctcat
     4501 taacttgttt catataacta cgtaaatttt ggcggaaatt tgaataagct actgcttcca
     4561 ttcctttcac cccttattta tttctataaa acaattgtac attatattgt acaattaagc
     4621 aaatgattaa tattctaact tcttat
//
LOCUS       DAZULO010000140         4609 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000140 DAZULO010000000
VERSION     DAZULO010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.145"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(84..398)
                     /locus_tag="ACG8OA_002699"
     CDS             complement(84..398)
                     /locus_tag="ACG8OA_002699"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4977433.1"
                     /translation="MLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQ
                     RYGATKIHQVLLKEGISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQ
                     KN"
     gene            complement(449..703)
                     /locus_tag="ACG8OA_002700"
     CDS             complement(449..703)
                     /locus_tag="ACG8OA_002700"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4977434.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(896..1171)
                     /gene="prgO"
                     /locus_tag="ACG8OA_002701"
     CDS             complement(896..1171)
                     /gene="prgO"
                     /locus_tag="ACG8OA_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFS4977435.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1143..2096)
                     /locus_tag="ACG8OA_002702"
     CDS             complement(1143..2096)
                     /locus_tag="ACG8OA_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4977436.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2708..4201
                     /locus_tag="ACG8OA_002703"
     CDS             2708..4201
                     /locus_tag="ACG8OA_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFS4977437.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4336..>4609
                     /locus_tag="ACG8OA_002704"
     CDS             4336..>4609
                     /locus_tag="ACG8OA_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFS4977438.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1541 t
ORIGIN      
        1 gcttgaattc ttgtgaaaaa gttcttcgtg ttctcttaga cataaaaatt cctcctggta
       61 tgttttcttc tagtctacac accttaattt ttctgtctag ttaattgtag cctatccatg
      121 atcggtttat tagacctttg aggtctatat tttttaacta caatcgacct taaatttaat
      181 tgtttcatta gcttttggac atgtttaaga gatactgaaa tcccttcttt taataaaact
      241 tgatggatct ttgtggcacc atagcgttgt ttattcgtaa aaaaaattgt tgaaatcttc
      301 tttttcaact tattatttct gatttctgta gctgtcaacg gtttattctt ataaaaataa
      361 tagacacttc tagggatttc taataaccga cacaacaaag aaacagtgta atccttacac
      421 catttttcaa taaattgaac aagcgaattt aggttttccg agagaatatg gctgcggctt
      481 tttttaagat ttcaagttcc tcatttaaac gagcattttc ttttctcagt tcagctactt
      541 cttttccagt cagtcctgtg gactgatcag gtaaatataa attcttccat ttgtaaatcg
      601 ttgcttcaga taagccatat tctttcgcta accctcgaac agaacgtcca gtttgattca
      661 gttcaacaat catttgttta aaattttctt catatcgggt cataaaaatt catcctctcg
      721 tagataagtt taatttattc ttgtctctac gaaaagctgt ctaagatttg atactaacac
      781 caaatgttgt atacccccaa acaatacaca tattttgaag gattaggatt aataaagcta
      841 aaaatatagt aagtatacta aatatattta ctatatctag tatacttact atattttaat
      901 cttttgatcg tctattatat ataatccgtt cctcgtcgtt taaagtatca acataggcgt
      961 ccattgcaag tttgactatt tcatacatag gaatatcttt tgcataacta agatcccgaa
     1021 tggtttttag tacgggagga tctacttgaa ctggttttct ttctttcgca gtgtactttc
     1081 gttcccctaa ctcgacatta atatcatctt taattgaaat atcttcagca ccaattttct
     1141 tcttatcttt gttcgttggt aataatgcca tttagtttaa tctcctttcc aagaggtagg
     1201 atatgattgg ccattgagtc agcatattta ggtgtataat caaatccttc aatatcacca
     1261 tgttgttcat agtattcaat tctttcttct aattctgtaa agatgtctgc aaaaatagcc
     1321 caccatcttc gatctacata atcatttaat tggataccat tctcaccata catttctaac
     1381 cgatccgagc ctttaacaat gcttttaaaa atgtttgatt caccatactt atcaattgtt
     1441 ttttgatagt ttgcgtgttg agaggaacgt cgcactgaga aaagaacagg caaaattcca
     1501 atgatttgaa gatgagactc ttcaaaattc gtgacaatcg gaactaaaac tttattaata
     1561 aaatcttctg ttccttccat tgcgtaccgt tttaattctt gcattgcaat aatataatct
     1621 gcagcagtta agaatgcacg aaccacatta tctgttgatg gtgggcagtc gataaagata
     1681 taatcgaaat tttttctcaa ttcttctaaa ggtttaatga aggccaatga tctcttacga
     1741 cgatcaggat atttttcgga caagtattca ttaagttctt cagatccagg agagccagcc
     1801 ataatatata aatgtggact tacttgagta atacctgaaa cccagtcccc ttttttgatt
     1861 gctgcagaca tggatcgatc aaagctagta atttgatacg ttgatccaat gttggaagta
     1921 gtatttcttt gttggtcata atcaattagc aacacgtctt tttcaaaaag ttgactagaa
     1981 acaatcgcta ataaatcatt gtctgtagtc ttaccaatgc cgccttttct acctgtaagc
     2041 attacaactt ttcctagttt atctgatgtt ttaaatgcag tccgtaagtt ttccatagtt
     2101 ccctccaatt tcctatacta aatatattta gtatacttca tatacttaat atattcgata
     2161 tatctaatat actccaaatt gataagaatt cagactttat aaggaatata gtggatatac
     2221 taaatatatt tgctatattc agtatatcag atatattcaa aatgtcaaaa tatatttgtg
     2281 cgttttgcgt tgacaattta gagtgaaaag aggtaaactt aattctatag aaaccacaga
     2341 taattcacta tttacaaagt agcaaaagca gtggtactct ttaaacagaa aaattacata
     2401 aaaaaaagag acggaaataa ttgattttag cagaatcaat tatttctgaa tcacatggtt
     2461 aggacatgtg ttcacgctcg tctcaagaat atgtaagtag ctaccaacta cttatattaa
     2521 aagttgatcg gatacctttt ccgattgcct ttattctacc aaaaaaaggc gagcaactca
     2581 acagaagaac gtttatttag tgtacttaaa ttaaatattc ttaaacactg aagagagaac
     2641 acatactatg gctgagatta aagctaaact gtgttctctt tttgtttgag aaaggacgtt
     2701 acaatcgatg ttgagcgaaa tttattcagc tattcttaaa aacggcttac gtacatacaa
     2761 atatcgaaac agcaccatga aacctgcagg tcatattgat tccagtagga gtggtgctat
     2821 ctttgcgtac cgtagcaagc aattaatgaa tgttgggcgt ggtatggtca ttacttcgga
     2881 agaagctatt ttagagaacg aaaagaaact aacgcattgg acacctaata cctatcgctt
     2941 tggtacttat gcggatgatt atcgaacagt ggttaaaggt cattctgaga aaaacttaag
     3001 tcaaatcaac acatttgtag ttgatatcga ttcaaaagag aaccatcaag gcgaaattat
     3061 tttagcctgt ttagatcaag tgggctacat gccaacgcta attttagaat cagatcatgg
     3121 ttatcaagtt tattttgtac taaagacacc aggctatgtc acaaagaaaa gcaatttcaa
     3181 ggtgatcgat gtagcaaaaa aaatttcaaa aacaattcgt ctacagttgg ctgaaaaatt
     3241 gtccggagtg gatctaggat gtaaccactt cggaattgct cgattcccac aagaaaataa
     3301 tattgttttt aaagaaatga actatcagta ttcttttgct gactggattc aatggtcgat
     3361 gaagcttgaa tcagatcaaa aaacaaaaga agatcgagaa cgaaaactaa ttgtctttcc
     3421 tgagaaaaaa gaaattcgtc aagtagatga accgtggttc gatatgttac ttcataaagc
     3481 aaacatcgtc ggcggagaag gacgtcttgg tcgaaataat gtgattttca cgctttcttt
     3541 agcttattac tcttcaggtt atgcattgga tacttgcgaa tacaatatgt tcacattcaa
     3601 cgaacggtta caggaaccgc tatctgaagc agaattagta aagattgtac gtagtgctta
     3661 ttctggtaat tatcaagcag caagcagaga attcattatt gaattgtgcc agcaatgggt
     3721 ttcaccaacc ttaaaaaata atgatctgtt cattcaacga cgtggctggt ggaaatttaa
     3781 aaagccacga gatcaaagaa aatatagtca taaaaatgaa tggaaagaag atctattggc
     3841 ttatctaagc gaaaaaagtc ttgctagtga gccttatttg aacctgtcaa aaaaagaatt
     3901 ggcttcacaa ctaacgatgc ccaagcgaac acttgataag atcttaaatg agttacgaga
     3961 ggagaataaa gtcttctaca aggtaacacg aggaagaaat ggcggaatga tcattgcttc
     4021 agtcaaaacg ttgttcgcga gggttattca actaagtaaa gaaaagagac tagattattc
     4081 aacaaggatc acagaggtat ttggactcaa cgagagctta gtagaagaga catttagaca
     4141 attatcaacc cccttgaacc ctcttaaaga agtgaatctt tttgaagtag atacaggttg
     4201 aaaaataaaa ggtgcaaatt gccttataca atatatctat gatacttctg taggtgttgc
     4261 tccctttgcc tagctacgtg taggtggtat acttataact gcgtatgcag tcttatgtat
     4321 catggaggaa gttgtatgtc gttaaaaaat tatgtctatt cacatcctgt taatgtgttt
     4381 atcattggtc gtttgggatt atctgtggaa gtgttctgtg agctttatgg ttttaagcag
     4441 gggactttgt cttcttgggt taccagggaa aaaacagttg cttctttgcc gattgagttt
     4501 cttcatgggt tgtccttagc ttctggggag actatggatc aggtatatga ttgtttgtgt
     4561 gtgttagagc aggagtatat tgagtataaa atagctaacg agttaagaa
//
LOCUS       DAZULO010000141         4585 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000141 DAZULO010000000
VERSION     DAZULO010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.146"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..409
                     /locus_tag="ACG8OA_002705"
     CDS             <1..409
                     /locus_tag="ACG8OA_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFS4977439.1"
                     /translation="EIIRKMYQKYLKTDIIYITNLEPEEYLTILKECKFMIGNSSSGI
                     IEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKIQDPQFIKSLKNVKNI
                     YGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            382..1059
                     /locus_tag="ACG8OA_002706"
     CDS             382..1059
                     /locus_tag="ACG8OA_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFS4977440.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1010..2191
                     /locus_tag="ACG8OA_002707"
     CDS             1010..2191
                     /locus_tag="ACG8OA_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFS4977441.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            2204..3181
                     /locus_tag="ACG8OA_002708"
     CDS             2204..3181
                     /locus_tag="ACG8OA_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4977442.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            3181..4089
                     /locus_tag="ACG8OA_002709"
     CDS             3181..4089
                     /locus_tag="ACG8OA_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HFS4977443.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            4090..>4585
                     /locus_tag="ACG8OA_002710"
     CDS             4090..>4585
                     /locus_tag="ACG8OA_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFS4977444.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVN"
BASE COUNT         1730 a          551 c          853 g         1451 t
ORIGIN      
        1 cgaaattatc agaaaaatgt accagaaata tttaaaaacg gatattattt atattacgaa
       61 tttagagccg gaggaatacc tgactatatt aaaagagtgc aaatttatga tcggaaattc
      121 ttcttctggg ataattgaag ctgcatcatt caaaatacca gtaataaatt taggaaccag
      181 acaaaatgga cgagaacaat ctaaaaatat tataaatgca aatctaaatg aaaatgaaat
      241 tgaaactgct ttgaataaaa tacaagatcc tcagtttata aaaagcttga aaaatgttaa
      301 gaacatctac ggtgacggca acagttctag aagaataaca aaaacattga atcaaatatt
      361 gaatgatggg gaagatatta agtggattca aaaacaaata gcttattaaa atatactagt
      421 gatccataca ttattattgg ggctggcgga cacgcaaaag tcattattga aaccttaaga
      481 ttagaaaata agttaatata tggatttttt gatgataagc aaaataaaat gcaagatctg
      541 aaatatcttg ggaaaataaa cgaattgaat agctgggcgg atagcaaaaa aaaaatctat
      601 aaatttattg tagctattgg tgactgtgta gtaagaaaaa agattataaa aaaattgaat
      661 atgccaagtg agcaatatgg aatagctgtt catccaagtg ctatagtggc aaaagaaatt
      721 tcaattggtt atggaactgt tatttttgcg aatgctgtaa ttaacccaga tgctgtaatt
      781 ggagagcatg ctataataaa cacaggtagc attgttgagc atgattgtag aattaataac
      841 tatgttcatt tatctccagg tgtatgctta gcgggtggag tacatgtagg tgtaggaact
      901 caaataggaa ttggttctca atgtatccag atgaagaaaa ttggatcttg gtgtatgata
      961 ggcgcaggga gcactattgt taaggatatt ccctcacatt ccttagctta tggcaatcca
     1021 gctaaaatta agaaagaagg aattaatttt gaattctaga attttattat caagtcctca
     1081 tatgggagga acagaagaaa aatatgttgc taaggctttt aaagataatt ggatagcgcc
     1141 attaggtgaa aatgtggata aatttgaaga aagtattaca aaatatactg gttctaaggg
     1201 cggagcttct gcgttaagtt caggtacagc tgccatacat cttgccttaa gactgcttaa
     1261 tgttggaaaa aacgatattg tctttgctcc ttcatttact tttatagcta cagtaaatcc
     1321 tattttatat caaggggctg taccagtgtt cattgactca gaacctaata catggaatat
     1381 gagccctgtt gcactagaga gcgcattaga agaagctgaa agaaacaacc aattacctaa
     1441 ggcaatcatc gtagtgaatt tatatggtca aatggctgat tatgaacgaa ttttagctat
     1501 tgcaaaccaa tttaatgttc cagtgataga agatgcagcg gaatcgttag gttctactta
     1561 taagggaatt tcgtcaggct tatttgggac aattgggatc tattcattta acggtaataa
     1621 gatcatcaca acctcaggtg gaggagctct agtgtctagt gataaaaaat atgttgagca
     1681 ttcaagattt ttagcaacac aagcaagaga tcaagcaccg tattatcaac atagcgaaat
     1741 aggttataat taccgtctaa gcaatatttc tgccggtatc ggacgtggac aaatggaagt
     1801 attgaatgat agagtagcta aacgaagaga aattttcaat cgatataagg aagctttttc
     1861 tggaattgat agaattgaaa tgatgccaga agtggatggc atggtgccaa atcgttggtt
     1921 gtctacttta acgatcaatg attataattc tattgatcaa gtagttaacg taataaataa
     1981 actaaatgaa aaaaacattg aaacaagagc tttgtggaaa ccactacatt tgcaaccagt
     2041 atttcaaggg gtaaaaatgt attaccatga taataatctt agtgtgtcag aaaagttatt
     2101 taatagcggc ttatgcttac cttctggaag taatatgaca gatagtgaac aagaatttgt
     2161 tattgatgag tttaaaaaaa tattcaagta ggtgtgatat tttatgaaca taggaattat
     2221 tggtttaggt tccatgggga aaagaagact tagactcatt caagaacact ttaatgatat
     2281 tttactatgt ggtgttgata ctaatgaggc gagacgaaag gaagttgaaa aggaatttgg
     2341 aataccaact tataatagtc taacagaggc aaaaaatttg tttgaaataa attcagtttt
     2401 tgtttgtaca tcccctataa cccatgaatt tattataaaa gaggctttaa atttagattg
     2461 taatatcttt acggaaatca atttattaaa tgaatattat gatgaaataa tcgaagtagc
     2521 taaaaagaaa gatagattac tttatctatc ttctacattt ttgaagagaa aagaaattca
     2581 atatattaag tctaaagtga cgcataataa aaaaatttct tatcaatacc atgtaggaca
     2641 gtatttaccc gactggcatc cttgggaaga ttatcgcaat ttttttgttt caaacaaaaa
     2701 aactaatggg tgtagagaaa tattcgctat tgaattacct tggattatag atacatttgg
     2761 gaaagttaaa tcgtttactg taatgaccca gaaattatcc aatcttgata ttgattattc
     2821 ggacagtttc actcttatta ttgagcatga aactggtata gttggaactt taagtgtaga
     2881 tgttgtttca agagtagcta aaagaaattt aactataatt ggtgaaaaga ttttaataga
     2941 atgggaaggt aacccggatt ctctattttc atggaatgaa gaaacttcta gtatgacaga
     3001 tattcaattg tacgatgagg ttatgcatga taataattat tcacatacta ttattgaaga
     3061 tgcatattta gaggagatta aagaattttt cacgcttttg gaaaagaaaa tggaacccca
     3121 atatactttt gaaaaagata agtatattat taacttaatt aacgacattg aggtaaatta
     3181 atgatgaaag taattttttt tgggttaggg tcaattggaa agagacatct aaaaaatcta
     3241 ataagctatg gaaaaaagaa ttctattaat tttgatataa cagcttatag tagcagtgga
     3301 aaaaaagagt ttgaagaaat aacgtatatt caaaatagag acgatttgga tacacattat
     3361 gatatagctt ttattacaaa tccgactttt aagcatctgg aaacactaaa tgtaattgtt
     3421 gaaaaagttg attacatatt tttagaaaaa cctgcttttt ctgagagtat tgatcttact
     3481 caatataatt atgattacaa aaatatttat gttgctgctc cactgaggta taaaaatatc
     3541 atggaagttg ttcgtaatag tattcaaagt aaaaaagtat attctgtaag agctatttgt
     3601 tctacttatc ttcctgattg gagaaaaggt actgattata gaaataatta ttctgccttt
     3661 aaatctatgg ggggaggagt agaattagac tgtatacatg aattggatta tattataaac
     3721 ctatttggtt tccctaaatc agtaaaaaaa cagtttggta aatattcgaa tcttgaaatt
     3781 aacagtaatg atttagcaac atatatattg agatacgatg ataaaattat agaaattcat
     3841 ttagattatt ttggaagagt tcctacaaga aaatttcaat tgtttacttt agatgaatgt
     3901 atcaatgttg acttattaaa taataaaata agttttgaag aaaacattgt taaagaaatg
     3961 cctgaatctg ctaatgatat gtatgagaag gagttgaaat atttctttga aaacgtagta
     4021 acaggcaaag aaaactggaa tgatttatat catgcaaacg aagtattgaa aatagctgag
     4081 gagaaataaa tggaaatttt atttactatt tgtggtagag ctgggtctaa ggggtttaaa
     4141 aataaaaatt tgaaatattt ggcaggaaag cccctagtcc atttcgcttt agcaatgata
     4201 gatatctatc aacaaaaaca tcctgaacat aattgcacga cttgtttgaa tacggacagt
     4261 aatgagttag tagaattgac gactaagtta aatccgtctg tgaaaattgt agaacgtaaa
     4321 cctgaattag ctacagatac tgtagggaaa attgaagtga tacgtgattc attcttacaa
     4381 ctagaaagag aagataatat atttgattta attatagatt tagatttgac ttcaccttta
     4441 cgtgaagttt ctaatcttga agaattaata gatgaacatc taaaaaattt agataaagac
     4501 gtctactttt cagttgtttc tgcaagacgt aatccctatt ttaatatggt aaaaagaatt
     4561 gaaaatgaag ttactatagt taata
//
LOCUS       DAZULO010000142         4559 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000142 DAZULO010000000
VERSION     DAZULO010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.147"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            655..1566
                     /locus_tag="ACG8OA_002711"
     CDS             655..1566
                     /locus_tag="ACG8OA_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HFS4977445.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            2053..2319
                     /locus_tag="ACG8OA_002712"
     CDS             2053..2319
                     /locus_tag="ACG8OA_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977446.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2391..2732
                     /locus_tag="ACG8OA_002713"
     CDS             2391..2732
                     /locus_tag="ACG8OA_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977447.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2787..3206
                     /gene="ssb"
                     /locus_tag="ACG8OA_002714"
     CDS             2787..3206
                     /gene="ssb"
                     /locus_tag="ACG8OA_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS4977448.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3283..3579
                     /locus_tag="ACG8OA_002715"
     CDS             3283..3579
                     /locus_tag="ACG8OA_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977449.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3594..4025
                     /locus_tag="ACG8OA_002716"
     CDS             3594..4025
                     /locus_tag="ACG8OA_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFS4977450.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4330..4512
                     /locus_tag="ACG8OA_002717"
     CDS             4330..4512
                     /locus_tag="ACG8OA_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977451.1"
                     /translation="MQRTLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKR
                     FTEEIEQLAELNIRYF"
BASE COUNT         1663 a          719 c          984 g         1193 t
ORIGIN      
        1 aaaggttata tgtaataagt ggggggaggg ctgtcggcgc ttggtatgaa aggcattctc
       61 cgacttccag gtttttgacc attttctctc aacgtggaaa aaccgccact tatgagagaa
      121 aatggtcaaa aacctaagcg ggaagtctcc gaataacata ccaagcgccg acagccaaca
      181 gataaagaat acactactaa aaatagtagt agcataaaat ttaaaaggga aaaaatcaac
      241 ccctacccca gtcttggggt ctcaaaacta actcccccct aaccccctct ttcagcctct
      301 tttgaaagag ggggagaatc aaccccacgg ccaacgcccc agtgttcttg gggctgtcca
      361 gaacagaaac ttgcgagaga gtgggggtaa aacaagtgta gaggaaactt gaagacttgt
      421 tcgaacagaa acttgcgaga gagccggagt aaaacaagtg tagaggaaac ttgaagactt
      481 gttcgaacag aaacttgcga gagagttgga gtaaaacaag tgtagaggaa acttgaagac
      541 ttgttcgaac acaaacttac aagagagggg agtaaagcag ttgcagagga atcaggaaga
      601 tttgttcgaa cagaaacttg caagaaagca ttattaaatt gaaggtgatg aaatatgaag
      661 tatttattgc atggctattt tattgattat tctactcgca atagtagtac tttagcgatt
      721 ctatgtttgc ttcatgaatt gagagtggtt actagaaaac agctatacaa actattgaat
      781 tttgacagat caattagtaa acaaggagta acgaatgctt tagtgagatt atatgaaaaa
      841 cagttaatcg atcgaaaaaa atatggaaca gaaatgtatt attatttgac gaaagaggga
      901 catcaaagca taggtgggta ttatacgttg ccgaaagttc ctgaatataa tttgaatcac
      961 catttacaaa ttaacaatta tttgattaaa ggaatggcat tggcaaaaga ccacccacat
     1021 ttaaaacgga ttatttcaga aagacgtacc gtctatgaaa cgaaggattt aaatcctaag
     1081 acaaaaggaa gaaaattttt tgtgccagat tttatttttc gttttcgaga caaagaaaat
     1141 catgagatca attggtcctt tgaaattgag ctaacgttga aaacacgtcg tcgctaccgt
     1201 gaagcgattt ttccgaaata tattcgtgag ttgcgaacac gtcctgaatc acatttaatt
     1261 tatgtgacac ctagtggcgc aattcaagaa gaacttcatg cgtttaagcg tatttttttc
     1321 aagcgcgaaa ctgaaaaaga tgcgagtcaa caagaattgt ttgagcggtt gcatattttt
     1381 tcagctttag agtttgaaga gggattaaaa gcattattag agaatgatcc agcagtcaat
     1441 tggcggacag tagaacgaga aaaaatttat ccgaatggcg ctgttcaaag tcgtttgaat
     1501 ttatttgaag aatcaactga aaatgaggaa aaaccaaaag tgaagtttac gcctgaagta
     1561 ttgtaaaaga gggtgttcgc ttcgctcttt ttttgtgcca gtttgctggc accgttcaga
     1621 cgcaagcgtc tgaaccggct cgtttttagg aaggactaac ttgtttgatt tatccgttgt
     1681 caaagaagtg gacgttcgag agtccctaat cgtatagcag tcattcggcg agtataacct
     1741 aaaacataac ctgccaattg ccaagggtaa aatgcacgct gcgcgccctt ggcaatcgtc
     1801 agaacatgtt ttcgagccgg ttatgccgaa cgaccgctat acgaaaaggg cctctaaaca
     1861 gaagtccact ttgatccttg acaactgaaa cccaaaatca aacaagcagg ccttcgtcta
     1921 aaaacgagcc gaatgaagac cttgggggtt gcacccccaa acccccgtgc ccccgacaca
     1981 ctaccgaaaa ggtgggggtg ggtggcgaaa aaatcaaata aaacattcta ttcaagaaag
     2041 gaagaaacaa ccatgacaac taaaaacgag caactgaaag aacaggcaag aaaagagttg
     2101 caagaaagag gtttgattat tgaggggagc tttgaaggag attttgaaac ctatattggt
     2161 tgttatgcaa gaccgataaa caaacctaca gccttagacc caatgaacga acaagaagta
     2221 aaagaacaag aaaaacatgc agtgaatggt tttccgcaag atttcactga atggtatgag
     2281 tgggaaatta acaacgggaa gcttgaaaac ttcttataaa agtcagatga cgtgcaaaga
     2341 gaatgattct ctttgtacgg taaaaatata aagaaagaag gaaaaagaaa atggcaaaaa
     2401 acacacctta tcaagtagga gacatttttt atagtagttg gggctacgaa caaacgaacg
     2461 tgactttttg gcaaattgtg aagttaacag aaaaaacagc atggtttcag cctattcaaa
     2521 agaaaacggt cgagcattat tcgtccatga gtggaaaagt tgttccaatg aaagacaagt
     2581 ttatagagta tcctttagca aaaactaaag acagtattgt aaaaaaacga attgatccag
     2641 aaacctatcc tgatatgtta tgtggttatc gtagttggga cacgtttcat agatataaaa
     2701 atcaagctgt ttatgaaagt agttattatt aaggggatag tttccccttt caaaaataat
     2761 aaatagaaga atggagagaa aaaatcatga tgaattcagt gaatttagta ggacggttaa
     2821 caaaagaagt ggatttaaaa tacacaccaa aaggcaatgc gacaggcaca tttattttag
     2881 ctgttaatcg aaattatacg aatgcaaacg gcgagcgtga agcagattat atccgctgtg
     2941 tcatttggcg gaaagctgcg gaaactttag caaaatatac tggaaaagga agtttgattg
     3001 gcattaatgg acgtcttcaa acgaggagct atcagaatca gcaaggacag accatgtatg
     3061 ttacggaagt tctagtgaca gatttctatt tgttagagag taaagaaatc aatgagcaac
     3121 ggtcaaaatc aatggcgaat aatcaaaata attcttcaat tccaggaaat gagattcaaa
     3181 tttcagatag ttatttgccc ttttaagtga aaaataattt tgttctatac ctgatttctt
     3241 caggtataga acaaaaaata aatagagatg aggcgatttt ttatgaaaat cattgacagt
     3301 tacgatcaac gaaccatgca atatttgggc tcgtttgatt atacagatga aaatattatt
     3361 aattatgttg caagtttacc tccttatcaa agcgttcgct tggtagattt tgcaagtgat
     3421 gaattggtat taacaacgat gggcaacttt ttagattatg tcggagacaa aaaatggttg
     3481 gaaaaaattt tgtctaaatt gattgcgaaa caaacaggag aagaacccat aaatgcgatt
     3541 caattgttca atcgatatga acaagaaaac aatgattaaa gaggagagaa aaaatgaaaa
     3601 gattaaaacg aagtattttt attgtgctag cattgattga ttttgttggg acaggttgtg
     3661 aagcaattga cgttcaaaaa gctcccttat cggaaataaa acaagcaata acaaaacaag
     3721 atgaagaaca accgctaaat acacaagtag aaatagtgaa tcaaaataaa ccgagttttt
     3781 ctgaagaaga tttttcgtta gaaaatggaa ccaggcaatt tttttcagat ttggatcgtt
     3841 taaatcgggt aggtgtagca agtgcacttt tgcataaaag caggatgcca acagaagaac
     3901 gagaccgatt atacattaag ccaactggct ggaaacaaaa gaaaatgaag aatggcgatt
     3961 atctttataa tcgttgtcat ttgattggct atcaacgtgc gacaagtttc cccataagta
     4021 tgtaatacga aacgggagga ttaatcttaa gattaatcac aactaaccta tttgacagtt
     4081 ggaatgaaag ctatcatgtc tgctgaatca actggtctga tactcctaca tgcaataaag
     4141 tctactagga agttattgta tgaagctagg taaaggcggc agaacaattt ctaagtgtgg
     4201 aatcatatga agttggatat gccgggacgc tataaattac tcagggtgag actgttttta
     4261 attaaactga cgaacttgcg aatgtacggg tctataaccg acttatattc gaaagatata
     4321 agattgcgaa tgcaaagaac attatcgaaa agtaagaatg ttggtcatga aattcgagat
     4381 ggaagactag cggtgtatct gtcaagagtt ggtaaaacat gtttgattga ttcagatgag
     4441 agtgggaatg cgacaaaacg ttttacagaa gaaatagagc aactagcaga actgaatata
     4501 cgatattttt aaatacttta gcggagaata aaaaaatcct ctacagaaaa ctacggagg
//
LOCUS       DAZULO010000143         4473 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000143 DAZULO010000000
VERSION     DAZULO010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.148"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            324..1052
                     /locus_tag="ACG8OA_002718"
     CDS             324..1052
                     /locus_tag="ACG8OA_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS4977452.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1197..3224
                     /locus_tag="ACG8OA_002719"
     CDS             1197..3224
                     /locus_tag="ACG8OA_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFS4977453.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3332..4429
                     /locus_tag="ACG8OA_002720"
     CDS             3332..4429
                     /locus_tag="ACG8OA_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS4977454.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1572 a          717 c          923 g         1261 t
ORIGIN      
        1 aaaatataat atacattttt tatttaatta taattaatat tgattgaagt ctaggcagtc
       61 aagtcacatt ttgatagata ggagggggac ttacgaatta ttttaaaggc tatgacagct
      121 gtatttctag attgtgtttg catcaagttg caataaaaac tagaaatcaa atcgggctga
      181 taatcattta taactaatcg atacatagga ccaaatatag gtctatgttc ttttatttca
      241 taagtatcgt aaaacagaat tttgctatgg attaatccaa tcgactgata tgtttagtaa
      301 ataaagaaag aggaatgtca atgatgaaaa aagaaatcgt attaacagca gcaatgatgt
      361 tattcagcat ggtagcaagt acgacttttg tttctgcaac tgaagtatac ccaaaggaat
      421 ataacacaga aggaacgatt acatttgaag caggagatga gggtgtaact cctcctgttg
      481 atccagagaa tcctgatccc aataaacctg tcgatccatc agatcctcca agccctggaa
      541 caggaggggc attatcgatt gattatggat caaaattcaa atttgggact caaaaaattt
      601 ctactgcgga taaaacgtat tatgcagctg ctgatgtgat gaacgatggg tctagaaaac
      661 cgacatatgt ccaagtgaca gataggagaa gtaccttatc tgggtggaaa ttaagtgtct
      721 ctcaaccaga acagtttaaa actgccagtg gggatgaact agttggagca cagctgaaat
      781 ttacgaaagg gcaggctgtt tctttggttg atccaacata tacgccgcaa acggtgaatt
      841 ctgaattaac gttgacaccg ggaggaaaca atacgttagc aattaatgct aaatcaggaa
      901 caggtgtggg gacttgggtc tatcgatttg gtgcaaatga aaatgaaaat caagatgcgg
      961 tacagctttc ggtacccggt aaatctgtca agttagcgca acaatattcc actaaattag
     1021 tttggacatt agaagataca cctaacaact agtttactga aaagaactgt taaaaataaa
     1081 tacgtagtca tattgtcttg cgaggtaatc gagcaaaaat tgtcaaaggc taggctaaat
     1141 ttgtttggat acgaatagtt catctaggaa tttaacgatc aggaggaaaa gatatattga
     1201 aaaagaggag gatcgtgctc attttaagca gtctcttagt gactaatatc tttccgatca
     1261 tagggtactc tttggaattg aatcagttgg agcatggaaa tagaagagac gaagaaagca
     1321 aactcacttc gacattcgac agtttaatag aatccaacca gacacaagaa agcagtgagg
     1381 atttaagtat ttcgagtagt ggagagacca ctgacaacgt agaagctgag ggttcggaca
     1441 aacaaggact agaagatgca ccaatttctg cggttgagct gcctgacgat cccaatatta
     1501 tttctatcga taaaattttt caaacaccta tcggtactag cacgtccata ttggaaaatg
     1561 ggaagttact gcagttgaac ccagcagaaa ggtctcaaag aggagctatt tggtcaaata
     1621 agaagattag tttactctcg gattttacat ttaaatctta tttatactta ggaaatcaac
     1681 gagatagagc gggagatggc atgaccttta cattgactgg cgatcctaga atggctagta
     1741 atcccagtca agttattgga tcgccaggta tgggaattgg ctcatattct actcgttctg
     1801 gccaacctta cattgcgaat gcgctctcga ttgagtttga tacgtataaa aatagtggtt
     1861 cttctgatcg aatggatcga gagataactg ctgataatag agggcatgga catgtcgctt
     1921 ttgtcacgcc taaaccaaat aataataatt attctggaga gcatactggc gtgacggtag
     1981 ctcctacatt tctttctaat ggcacatggc gcttgttaat aattaaatgg gacgcggagc
     2041 aaagaagtct tacttatgat ttggaaggtg tcggtcactc ttctcggatt atacctgatc
     2101 tcattaggca gtttggttct acagaagtaa attgggggtt tacttcttca acaggtactt
     2161 tttttcaaga aaatgctgtc gctgtcacgc gacttccatc gaatgtcaca tcaacagctg
     2221 atgtaaaagt caacgatagt gattatgggg ccatcgctga agctggagga aacgataaga
     2281 taactatcag aaatacattg aaggtcgagg gggattttct tgaatataag aaaatgcaga
     2341 ctagtatcga cctcccacaa gaattaacta ttcccgagca tatcttgatc aatggaaatc
     2401 aaattgatgc caaagatatc ttgataaccg gaaatcgagt cacgctaaat ctgacagatt
     2461 attttattgc tggagatacg ttaataatcg aattggagac aactttgcag gaagtaattc
     2521 ctgaaaaagt actcactatg cattttgaat accttgaaaa cgggaatttg attgagcaat
     2581 cgaatgaaat aaacctaaag atcgcaaagc tgaaggagat agatatccat gtttattata
     2641 aagataaaga cagtcagatt gatattgctg aaatgaaaac aatcactggg aaaataggag
     2701 agaagtataa agaaactcct attgatattg aagggtatgt ttttgactcg gattcagcga
     2761 atacagaagg catcttctcc gaaaatacag aggatatcta tttttattac agaaaaggca
     2821 aactttatct ctcaactgct cctcaatatg tcaattttgg aaaacataaa atatccagta
     2881 gaccattaag tgaattcgga cagttttcag gtgggttgaa aattatagat gaacgggctt
     2941 ttggaagttg gaaattacaa ttgaaacaag agaaagttct tacaaatgga gattttgagc
     3001 ttccagaagc tctttccttt gttacctctg aaaatacaac ggttataggg acatccgctg
     3061 ttacgatttt cgaaagtgat caaaagggtg aaagcgattt gtcctctctt ttagattcta
     3121 cagctcatcg tggaatcaga atagatattc ctgtagaaaa ccaaagaatc ggaacctttg
     3181 aggggaaact atcttggata cttgcagatg tcccaggaaa ttaacaggaa gagaagaata
     3241 taacaaaatt attgagtttc aagaagggca caggtaccat aggctcttgt gtcccacaga
     3301 gacaagattt tgttatagtc ttctcttaca tatgattaaa ggagcgattt tcttgagacc
     3361 aaaacaacaa ttatttataa cactcctagt tattttaggg gcacttttta acaatatatc
     3421 agtgtcagct agtgcattta atttttcagt gacgccgata acttcagaga atcaaatcga
     3481 caagagaaaa acttacttcg atcttcaact tgatccggat caggaagtag aagtaaaagc
     3541 ggaattgaga aatgatacag aaaaagaagt gaaaatcgat atttctgtga atagcgcaac
     3601 caccaattca aatgtgatgg ttgaatacgg aaaaaacgag attgaaaaag acatgagcct
     3661 gatctttgat ctggtagatt atgtgagcta tccacagata gtgacgctaa aacctaaaag
     3721 tgtccaaacc gttgcttttc atgtcaggat gccgaatgag cgatttgatg gtgtattggc
     3781 tggtgggatc acattccaag aaatccagac ggaaaaggac caaacagaaa ctaaggatca
     3841 aagcttatcg attgagaatg aatacaccta tattattgct ttgttgatgc aacagacact
     3901 gaacgaagtt gctccgaacc ttactcttca tgaagttaaa ccagatcaaa tcaatgcaag
     3961 aaatgtcatc ttagcaaacg ttcaaaatga tcaaaaaacc tatatcaatc aagtagttat
     4021 tgaaacaaaa attacaaaaa aaggacattc tgaagtttta tatcaagagg aaaaagaagg
     4081 attgcagatt gcgccgaata caaatttttc ttttccaact gctttaaatg gtcaaccgct
     4141 aaccccaggt gaatatcacc ttacaatgac tgttttagga aatgaaaatg aaaatggaaa
     4201 attttcgaga aaaaaaggaa atactacgat caactatacc aatcaatggg tatttgaaaa
     4261 agattttaca atagacggaa aagtagccaa ggaattgaac acgacggatg ttacgattaa
     4321 aaaagacaat aattggattt atatcttagt agcttcactt ttcttattgt ttattttatt
     4381 atgcatactt ttgtttgttt tgagaaaaaa agaagagaaa gaaaagtaag gttaagataa
     4441 caaatagtaa atttttatta ttgtaggatg gtt
//
LOCUS       DAZULO010000144         4434 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000144 DAZULO010000000
VERSION     DAZULO010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.149"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(543..2048)
                     /locus_tag="ACG8OA_002721"
     CDS             complement(543..2048)
                     /locus_tag="ACG8OA_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4977455.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="ACG8OA_002722"
     CDS             complement(2045..2731)
                     /locus_tag="ACG8OA_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4977456.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACG8OA_002723"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACG8OA_002723"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFS4977457.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1547 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attt
//
LOCUS       DAZULO010000145         4425 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000145 DAZULO010000000
VERSION     DAZULO010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.150"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            690..1658
                     /locus_tag="ACG8OA_002724"
     CDS             690..1658
                     /locus_tag="ACG8OA_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4977458.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="ACG8OA_002725"
     CDS             1669..2484
                     /locus_tag="ACG8OA_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFS4977459.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="ACG8OA_002726"
     CDS             2552..3262
                     /locus_tag="ACG8OA_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4977460.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="ACG8OA_002727"
     CDS             3298..>4425
                     /locus_tag="ACG8OA_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFS4977461.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAZULO010000146         4418 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000146 DAZULO010000000
VERSION     DAZULO010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.151"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..913
                     /locus_tag="ACG8OA_002728"
     CDS             59..913
                     /locus_tag="ACG8OA_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS4977462.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="ACG8OA_002729"
     CDS             1010..3259
                     /locus_tag="ACG8OA_002729"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS4977463.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..>4418
                     /locus_tag="ACG8OA_002730"
     CDS             3262..>4418
                     /locus_tag="ACG8OA_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4977464.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
BASE COUNT         1556 a          625 c          807 g         1430 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga tatcggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acgggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actagagt
//
LOCUS       DAZULO010000147         4348 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000147 DAZULO010000000
VERSION     DAZULO010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.152"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(387..1853)
                     /locus_tag="ACG8OA_002731"
     CDS             complement(387..1853)
                     /locus_tag="ACG8OA_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4977465.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /locus_tag="ACG8OA_002732"
     CDS             complement(2144..4228)
                     /locus_tag="ACG8OA_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4977466.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1415 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaattt
//
LOCUS       DAZULO010000148         4327 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000148 DAZULO010000000
VERSION     DAZULO010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.153"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..283)
                     /locus_tag="ACG8OA_002733"
     CDS             complement(<1..283)
                     /locus_tag="ACG8OA_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFS4977467.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENI"
     gene            complement(732..2140)
                     /locus_tag="ACG8OA_002734"
                     /pseudo
     CDS             complement(732..2140)
                     /locus_tag="ACG8OA_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(2361..2723)
                     /locus_tag="ACG8OA_002735"
     CDS             complement(2361..2723)
                     /locus_tag="ACG8OA_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977468.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2727..3035)
                     /locus_tag="ACG8OA_002736"
     CDS             complement(2727..3035)
                     /locus_tag="ACG8OA_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977469.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3044..3463)
                     /locus_tag="ACG8OA_002737"
     CDS             complement(3044..3463)
                     /locus_tag="ACG8OA_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977470.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3456..3689)
                     /locus_tag="ACG8OA_002738"
     CDS             complement(3456..3689)
                     /locus_tag="ACG8OA_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977471.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3686..>4327)
                     /locus_tag="ACG8OA_002739"
     CDS             complement(3686..>4327)
                     /locus_tag="ACG8OA_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4977472.1"
                     /translation="NPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFIT
                     QYRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWW
                     RLELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAK
                     NTKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1224 a          962 c          620 g         1521 t
ORIGIN      
        1 caatattctc ttcatgttgg tctaacaact gccaaccatt attactaaca gatcttaaat
       61 aaccattacc ttcatttagg cgcataagct gttcaaaatt taaaatcaac acatcatttg
      121 gagtgtgata ataaatattt tctttcagtt ttccgactat ggtaattcgg tcaattgacc
      181 actgcatttt taagggaagg gtcaaacttt gatactttaa ccgcctgtta gagggggctg
      241 ttaatttttc ccactcattt atgatcgtgc cttttttact caaattttgt tcctcctcaa
      301 agaaaaagag acttctggaa agaattttca aaagttcacg taactcttaa aaattcatta
      361 ccaaaaaatc tctagtcttg aagaaatcta aaaatattcg tataatgatt aagaagttac
      421 ggagctagtg cgattagttt ccgtaaaggt cgagaacgtg tccagcgttg ctcgacttta
      481 tttttttgct cttttttcag tttatttatt tgataactca ataatagcac ttctctttag
      541 ctatgcaaat atccaaaaaa taaaaccgca ccctttgaaa agggcgcccc caaacttcgc
      601 taacgctagg ttggccaaaa aatggacttg ccatttttta gcaaatgtca gcttcaggga
      661 ttagtggttt agccgtcaac tccttatcct cggcacaaac cgcctccggt accgttgact
      721 gcacatcgta actaccactc gatctcatct tcagaacgct ctaatttacg ctgtgagacg
      781 ttttgacgat aaaataaggt tttctcctgc tctaacactt ctaattcctc agaaagcccc
      841 atatcctttt ctgcaagacc aaaacggata gacaaacgag tgatcgattt ttgaaatgcg
      901 ttttttaatc gctctaaacg ctggcgtaaa gagcgatttt cttctttcag ctttttattt
      961 tccacttgta aatctgctaa ttgttttcta ccagacaaat gacgctcttc ttccaatgta
     1021 gctacatact catttaattc tgtattttgc tcaagcatct gtaaatattg ctcactcatg
     1081 ttatcagcat ttctcttggc atttgttgct ctcgtagctc gttgatacaa atcaacaaaa
     1141 tcattcccag ataaatgata cgaaccatca ggaagcctct tgaaactttc taaaggaaca
     1201 atatcttttt ggaaaatgac cgccatctct ttttttacat tcagcatatt tttcttctct
     1261 tcttcaatct tcactctctc agaagccacc agagcctttt ctcgctcatt ttcttctctt
     1321 tgcctagaaa aatatacctc aactcatcag aaacgctctc acgctctttt acggccgttt
     1381 gacgcaattc tgaaatctcg ctcatgattt ctttatattc gtgaacatct ttgattttgt
     1441 tagtaccgac taactcacgc tcccaaccaa aatctgccat caaacgttca attgactcaa
     1501 cttcctgatt cctaaaatta ttccaaatca ctcgaccatt ctctttatct gctcgtaaac
     1561 cctgctgttg caaggctttg ttaaaacttg gttgcttttg aacaccccgt ttataccctt
     1621 cagcaacagg aacaatattc aaatgcaggt gcggacttac ttcgtcatta tgaataacag
     1681 cattgtagac ggataaatta ggattccgtt ctttaaattc tttgaaatat tcaactaaaa
     1741 ttttgttggc aatcactcga ttttgttctg cttgttgttc cgatatgcca gtagaagaac
     1801 catcatcacg agtagtacga aaatcatcaa gagtaccaat ttgcacgata aattctcgct
     1861 gtggttccaa ttttttatcc ttgcgaacct ttgctaaata gtcctcgatt tttctatcgg
     1921 ctcttttctg ctttgcatta tatttttcta cggcttcacc gaataattct tcatattttt
     1981 cacgaatatt tgtttgttct aaatagatat tttcatccga ttttgtgaaa tcaatatgct
     2041 tatgccagtc attgattttt tggctatcag ttaactcacg attattatga gaaatgcttg
     2101 tattctttgt tccctttttg aacgaaatac tgaaccccaa gttaaaaccc tccttcggct
     2161 gtcgctaata aaaagagtgt aaacctttct gttactcttt gtcaaagagt aacattttcc
     2221 taattgtgac atggctgtca caattccaaa tgctctccga gggtattcgc tgtcacaagt
     2281 gacaacgaaa aaaggagccg aaagcgtgtc acgctctcaa ctccttttac gctttatttt
     2341 gcttcacgca aaataatcgc ttatcgtaaa ttcacgtcat taaatattga cgtaaattta
     2401 ctctcactca tctgactttt cttattcggt atcactccga ataagccgtc agattcatcg
     2461 ggataatctc gttcgcttgg cgctgatttt tcatcagcaa gccactcatt atccactgat
     2521 ttttcgtgtt gctgatcgtc agaaatcgac gatagccacc gctcaaaatc agcaaaagtt
     2581 tcaaacccaa aacgcttccg aagagccttg cccattgcag cattttcttt ctcaatcaag
     2641 cgttttttct gttggcgaag tttcgcttgt tcttttttta atttttcttc ttgcgcttta
     2701 atatctgcta aagtcattcc cataaactac atctcctttt ctttggaatt aataacggaa
     2761 atttggttta actgcataac atgactcatt aatttcaacc aaaattcttt ttctgattca
     2821 tcagttgttt gcgctaaatg cttatgaaca aattcattag tcaaaactga agattttgct
     2881 aaatcagcat tgataaccga gttgagaaca tccataattc gttcaagcat ttcactataa
     2941 ttatcttcat ccggcaaatt caaataatca gctaaccgtt catacatcaa ttcaattcta
     3001 tcgactgttt cttctctcaa atcaatttta tccatgtcac acctcacttc cagaattgcc
     3061 accattttcg attttttaat tcttgatttt ctagtaacaa ctgatcgttt ttttgttgct
     3121 gaagcagggt caatttttgt tgatcacgaa tcatttctgt taattcagaa atatgtttgt
     3181 ccttctcagc taattgatca cgcaaaaact cattctcttt ttctaaagga aaatcactgc
     3241 ctgtgttatt gaccggtaat tgaccagtca attgatcact ttttgaccga taaattgacc
     3301 gaacaacacc aatcaactct ttatctagca tgataatgcc attttcttta tactgagcaa
     3361 ggtcaaaccc tgaattgacc agttgattgc gcactgtatt tttcgataca cccaattcgt
     3421 cagcaagatt ttttatggtc atcttttctg acaaatcagt cattagcttc tccactactt
     3481 tcagctaccg cataaatata tttaacggcg tgcttaggga taataatcgg agagtcaatt
     3541 ttattatttt tcaaaatcaa ttcatattgc gttacatcaa gtaacttacc ggacattttt
     3601 ttgctatcca aaaaaacaac cacaacgtaa tcacctgtta aatttaccaa ccaatttttc
     3661 ttaccaaaat acttttgttt ttctgtcatt tttcttcctc ctctgttacg tctagcccta
     3721 gaagccatgt gtctaattct ttttttaaat cgtctgccga ttccgcaaag gtttctcgca
     3781 aatgattcgt caattcatca ttttgacttt cttgcttcaa caaatccctg tattttttct
     3841 ttgtattctt agctagattt ccccactcag aatgatcata aagcaaacct ttcaccataa
     3901 tccgttcagt aactttagta tttggcggaa aataatgtgg actagcaaag ctatccaaac
     3961 tttcatgaac catctcatgc caatcagttg cttttcccct tcgtaactgc aattctagtc
     4021 gccaccatgt cgttatttcc ggcggaacaa ttttacgttt tctttcttgt tccagttttt
     4081 tattatacat ccgaatttgt cgttcactgg cacgactacc ccaatatgcc gtttctaact
     4141 taccagaacg cccataaatt ggtttaaaag acacaggatc aactacccga tattgtgtaa
     4201 taaactcatc aggtacatcg ataatgtcgc aagcaatatc cgcacgtgaa aagtgaggtt
     4261 cgataaacaa atcatgaata aaatttttca tggaactagc taagaattga ctaatcttat
     4321 tcggatt
//
LOCUS       DAZULO010000149         4282 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000149 DAZULO010000000
VERSION     DAZULO010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.154"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..1257)
                     /locus_tag="ACG8OA_002740"
     CDS             complement(37..1257)
                     /locus_tag="ACG8OA_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS4977473.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ACG8OA_002741"
     CDS             complement(1277..1888)
                     /locus_tag="ACG8OA_002741"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFS4977474.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ACG8OA_002742"
     CDS             complement(1931..2863)
                     /locus_tag="ACG8OA_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFS4977475.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="ACG8OA_002743"
     CDS             complement(3284..4144)
                     /locus_tag="ACG8OA_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4977476.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DAZULO010000150         4251 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000150 DAZULO010000000
VERSION     DAZULO010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.155"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            118..558
                     /gene="fabZ"
                     /locus_tag="ACG8OA_002744"
     CDS             118..558
                     /gene="fabZ"
                     /locus_tag="ACG8OA_002744"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS4977477.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(673..1434)
                     /locus_tag="ACG8OA_002745"
     CDS             complement(673..1434)
                     /locus_tag="ACG8OA_002745"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4977478.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            1623..2717
                     /locus_tag="ACG8OA_002746"
     CDS             1623..2717
                     /locus_tag="ACG8OA_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFS4977479.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(2803..3762)
                     /locus_tag="ACG8OA_002747"
     CDS             complement(2803..3762)
                     /locus_tag="ACG8OA_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFS4977480.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         1345 a          718 c          812 g         1376 t
ORIGIN      
        1 ttgattgtca aactattttt tcttaaatta aaaaaagatt gctaaatatt taggaatatg
       61 atactttgat agtcaaagta atgaaaatgg caattgcctg aaagaaggag tttaattatg
      121 tcttacgtat tatctgctac tgaagtaatg gaattgattc ctaatcgtta tcctatttgt
      181 tatattgatt atgtagatac tttagaacca ggaaaaaaga tcgttgcgac aaaaaatgta
      241 acaatcaatg aagatttttt ccaaggtcac tttcctgaca atccagtgat gccgggtgcg
      301 ttgattctag aaacactggc acaagcaggt tcgatactta tcctaaaatc agaagagttt
      361 ctaggaaaaa gagcttatat cggtgggatc aataaggcca aattccgtca aaaagttgtt
      421 ccaggagatg tgctgaaatg tcattttgaa atcaccaaaa tgaagggatc agtcggtaca
      481 gcgttatctg cagcatatgt agatgataaa aaagtctgtg agtgcgagtt cacgttcatc
      541 gtcaaccaaa atgcttgact ataaaacctg atatagcaaa acaaaaggaa gcttacttgc
      601 tttttatgta aaaagaagac gcctgaatcc tagcatacgt tgtcatctat gcaggattca
      661 gacgttattt ttttattttt gttttgcttc ttgcagtata ttgattttat cgatttttac
      721 ttcttctttt ggtttgtctg aactatctgt ttcgatattt gcaatagcat ccacgacatc
      781 cattccttca atcacttggc cgaagacagt ataatggcca tctagtgaag gataaccgcc
      841 gtttttataa gcgtcgatga ttgcttgagg atagtcgtct tttaaaagac catcatgcat
      901 atcatctgta ttttgaacga taaagaactg gctgccgtta ctgttaggat cttgtgagcg
      961 agccatagat aacgcacctc ttatattata aaggtggttg ttgatctctg tttcaaatcc
     1021 ttctccccaa atgctttccc ctccagtgcc atcgccttta ggatcgccac cttggatcat
     1081 gaaatctttg atgacgcgat ggaaagtcag tccgttgtag taaccgtctt ttgcatgtgt
     1141 cataaagttt tcaaccgctt ttggtgcgtg ttcgggaaat aatttgattt tgattgtgcc
     1201 tttattcgtc accatttcga ccagatcttc attttcggcg acatctttcg tcaattgtgg
     1261 taattccaat gcgttcaaat cgacgctact ggcagtcgtg ctttccgcag tagatgtact
     1321 ttttgaactt tctgtagacg aactagaaga agtcgtgttg tttgcagaac aaccagcagc
     1381 aaacagcaat gctgctgcta aactaatact taaaataact ttatttttct tcataactgt
     1441 tcctttccaa tttactattt tgttatcttt aatgataaca aaatagaggg aaaataccta
     1501 cttaaattat tggaatttat catttatttt aaaagaaaaa gaaaaggtgt agaccattta
     1561 ctttgtgttt gctataatgt aagtggatac aaaaacgaaa agatgcagga ggaaatggta
     1621 atatgggaaa attaggaatc tcgatttatc cagaacggtc aacatttgaa aaggacaaag
     1681 cgtacttgga tttagctcat aagtatgggt ataaacgggt ttttaccagt ttgctgcaga
     1741 tcaatgatga taaagaaaaa gttctttcag aatttaaaga agtagttgat tacgcgaata
     1801 gcctaggcat ggaagtaatg gttgatatca atccagcgtt atttgaacag ttggagattt
     1861 cttacgatga cttatctttt ttccataaga tgggtgccta cggggtccgc ttagatatcg
     1921 gattcacagg agcagaagaa gcaaaaatga cgcgcaatcc ttttggtatc aaaatcgaga
     1981 tcaatatgag ttcgggcacg aattacgttg acaacatcat gagttattcg ccaaacacgg
     2041 ataatttatt aggttctcat aatttttatc ctcatcgata ctctggttta ggatacgaac
     2101 actttgtttt ttgttcagaa aaattcagaa aatataattt gaatacgatg gcattcgtca
     2161 attcgcaatc tgctgagttt ggcccttggc ctacgcaaga tggcttgtgc acgctggaag
     2221 accaccgaga tcttgaaatt gcgacacaag tgaagcattt aatccttaca ggattgattg
     2281 atgatatttc tattggaaat gcgtacgcaa gtgaagaaga attgaaagaa atggcagaag
     2341 catttaacgc ggattatcca acattgaaag tagatacgga agaaggtatt acagaaaacg
     2401 aacgaatctg cttgtttgat aaccttcata gttatcgagg ggatcgctca gaatatatcc
     2461 ttcgttcaac gatgactcgt gtatactaca aagataaaga tttcccgcct cataatacgc
     2521 gagatatgca tcatggggat gtactgattg acaatgaagg gtacggtcaa tacaaaggag
     2581 aaacacaaat cgccttgaag gatatgaaaa acgacggacg tgtcaatgtt gtaggaagaa
     2641 tctccgatga cgaattattc ctccttgact ttttgaaacc atggtcaagc tttaaattaa
     2701 ttgaaaataa aaaatagata attagttatt gcagaaataa tccgctagaa atagccagtt
     2761 atttctgctt ttttattttg tttttttata ggttatgatt tgtcaaatta agctagaata
     2821 aaatgcttct tgtacatagc tcaaaaatat ttcgattggt gttctgtaat tcaatgattt
     2881 tcttggaata tgattacgtt gattgctgac actggaaata aatgtctgat tcacttttct
     2941 aaaatccatt gatttcggca gtccattacg acgcagaatc ccgttagaat tctcgtttaa
     3001 tgggcgttga gaaggtgttc cagggtccgc aaaatatata tcaatatcat gttggttact
     3061 aatggctttc cagttagaaa attcttttcc acagtcaaac gtaatagatt taaagaagtt
     3121 tttagggaag cgagaaaacc attgattaag ggcagtttca atatctaatg ccttacggcc
     3181 gttgggttta atcgtgataa tgactttaga taatctttca actaaagtaa tgacggcact
     3241 tttatgatga atgccaacga tcgtatcacc ttcaaggtga ccaaactcag aattgaaatc
     3301 aggataatta tcaggtcgat catggattga gcgctgatac tgttgttttc cccgtttttc
     3361 ctgatggcca ttgggttttc ttttaccttt catcggtagt gtgtcaatat caaatattcc
     3421 tttagaaaat aaacgataaa gtgttctcat accgcatgaa acaggccttt cttttcgccc
     3481 gataatgacg tcaggcgtcc aaccaagagt gactttctct ttaatgtaat ctacttcatg
     3541 agcaggtaat tgaatgactt ttctaccaca ttttttctta ttttctttat actggtgcca
     3601 ataatcaaga gcagtcttgc cttgcttgaa cttattgact acattataaa tagtttgtat
     3661 agcacgtccc attcggttag cgatttcaac cggcttatta tgttgaagat aatatgattc
     3721 tatcattgtc agttcgtcta tggtaagatg tttgtaggtc atttatggtt acactccttt
     3781 gttttctttc gtcggaaata caatttgagt gtaccataaa tgccttttta tttttctaac
     3841 ttaattttac aattcgcgat atattaaaca acgttgtcgc gcttcattga aacattttat
     3901 gaaaattcca aaaaagagtt gacacatgtt tttagacgtg ataagataac taagtcgtta
     3961 aaaagaacca cttgcgaagc ttgattctat cgaaaaaaaa cttcaaaaaa gttcttgaca
     4021 aataacaaac gataagttat gataaatgag ttgctgaaag atttcgaaaa acgaaatcga
     4081 aaaaaacttt taaaaaagag ttgacaatcg cttgtctctc tgatatgata taaaagttgc
     4141 tacggcagcc aaaaacgaat cgggaaacac tcccgatgaa gtagaccttt gaaaactgaa
     4201 caaagtaaga caaaccaaat gtgtagggcg tcttgattca attcaagaca a
//
LOCUS       DAZULO010000151         4217 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000151 DAZULO010000000
VERSION     DAZULO010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.156"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            405..926
                     /locus_tag="ACG8OA_002748"
     CDS             405..926
                     /locus_tag="ACG8OA_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977481.1"
                     /translation="MDKPFLDSVREYLSNPKSTLFPHEDYKIIMNYYLPIGAPQNSKI
                     KIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGL
                     DLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLM
                     ELKKYSNTQTKFS"
     gene            1042..1362
                     /locus_tag="ACG8OA_002749"
                     /pseudo
     CDS             1042..1362
                     /locus_tag="ACG8OA_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1540..1899
                     /locus_tag="ACG8OA_002750"
                     /pseudo
     CDS             1540..1899
                     /locus_tag="ACG8OA_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            1963..>4217
                     /locus_tag="ACG8OA_002751"
     CDS             1963..>4217
                     /locus_tag="ACG8OA_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4977482.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFS"
BASE COUNT         1547 a          574 c          774 g         1322 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa tacgttatgt agtaaatgta atagtttttt tggtagtgag tatgttgagg
      241 aatatattag atttgcagaa gacaataaaa atttccttta taacaataca agtttaaaga
      301 atggtaagtc tgatttaaca cattcaataa agaaaatgaa ttcacttaga gttgcaaaag
      361 caattgtagg aatgtttttt agtttaaatg gggatgaaga ttcaatggat aagccattct
      421 tagattcggt acgagaatat cttagcaatc ctaaaagtac tttatttcct catgaagact
      481 acaaaattat aatgaattat tatcttccaa taggtgctcc acagaattct aaaatcaaaa
      541 ttcctaatga cattgtaaat gatggtgaca ctcacaatgt aataaaaggt tttctgcccc
      601 ttgatctatc aactgggaga aaaattcgat tttcagaaat tcagtatgaa ggaattggtt
      661 ttactcttat agatttaaaa aattcaactt ttgaaataaa agaaggatta gatttaaaaa
      721 atctcctttg tgcagatgat aaaatcaaaa aaatacaatt gtacaaagtc cctatattaa
      781 caccaacagt acttacaatg ttggagatgg aaaataatat taattatatt gactcaggtt
      841 cagatttgct gattaggtgg caggtaataa ctaattttct tatggaatta aaaaaataca
      901 gtaatactca aacgaagttc tcttgaagtt ataaagaata caataaatat aaatttcctt
      961 tagactatat taaaatcagc aatcgaagga aaataaaaga agaaaatggt acgcaaaatc
     1021 taactattag agcaaaaaca ggaagctgct ttggaagata gtcccgaatt gtttccgatg
     1081 ttaaaagatc gtttaaatga attgactgaa aaacgaagac agttacaaat tcgagagaat
     1141 gaaatcttag ggatctttca acaacaaggg gaaccgattc aagtcaaaga tgtccaacag
     1201 atattgacta gtttggattg ctttcttatc cagagtgaga agaagcaagt taaagctttg
     1261 tatcacacct ttattgagaa aatcaccttt gatcctaatc agaagtagat attgccgatc
     1321 ccgatgagat tcacacctac tgtgatttga taattgaatg agtaatatca aatagccgta
     1381 tccaaaccaa aggatatggc tatttttgtg ttagggatat acgtcacgta gcttgttatc
     1441 aagaataagt ttctttattt ttttgctttt tctaaaactg agtatgctaa aataagaggg
     1501 tatatagcaa ctttaaataa gagataggag tgtatctttt tgggtgcaga attaaatcag
     1561 aagttattta gtgcggcaga taatctccgt agtaaaatgg atgcgtcaga atataaaaat
     1621 tatttattgg ggctgatttt ctataaatat ttatccgacc gtttgttgga acaagtggtt
     1681 cttttagcag atgaatcctt agaggaatat gacacagtaa gcaaacaaac gatgttgtat
     1741 cgtgaattac tttcagatga agaatcaaaa gaagatctga ttgcaacaat cgttgatatt
     1801 ttggggtatg cgatttcgcc agagtatctt ttcaacgtct tagcggatca agcaaaacaa
     1861 gcaacgttcc aattgaatga tctaaacaaa gcctttttag caagtcatat ttatggaaaa
     1921 atactgtcgg caaaattcga atagtgtcga ggaggtgctg tgatggaaga attaaaagaa
     1981 cgcattgtca attatgtcat agagaactct gagattaaaa ataaatgggg gaacaataca
     2041 gcacagaaaa tatctgaaaa attaaatgtt agtcgtaata ttgtctctca atatttgaat
     2101 gaattcgtaa aagatggaga atttggaaaa atcaatactc gtccagtaat cttcttttct
     2161 ttaccggaga aagttccaaa tcaacaaatt tttagctctt cagaggaagc ggaaaaatgg
     2221 tatgatagct cttttagggt tggaatagaa agggttatcg gaagtcaagg aagtctgcga
     2281 aatgtaattg ctcaaattaa agcagcaatc tactatccgc caaatggctt acccatttta
     2341 cttcatggcg agactggaac tggaaaaagc tatttagcaa attatgtgta taaggatttg
     2401 ttggccaata attatataga agagaaggca tctttcattt cagttaattg tagtgaatat
     2461 gccaataatc cagaactttt cttgtccaat ttttttgggc atgtaaaagg tgcatataca
     2521 ggagccgaag atggaagaaa gggtttagca gaattagcta ataatggagt cttattttta
     2581 gatgaaattc atgcattgag caatgaatgt caggagaaac tttttcagtt tatggatacg
     2641 aggaaatttc atcggatggg tgataatgaa aactggtaca catcaagttg ctggattata
     2701 tttgcaacat cagaaccacc tgaggataaa ctattatcaa ctttgttgag aagaatacct
     2761 gtaaagctaa cacttccaac attagacagt cgttctcttt atgaaagaaa acagttaatc
     2821 aagatatttt ttaaatcaga agaagaaaga ctagcaaaga aaatactgat atctcaacaa
     2881 ttattagatt ttttattaaa ctatcgcttt agtgaaaatg ttggcaattt gaaaaatatc
     2941 attcaattga cctgtgccaa tgccttttat ttcttagatg atttggaaga actttatatt
     3001 cagatgtatc atttaccaca atgggtgaat ttcaacggca tgaaggttat gaaaagacag
     3061 aatgaattaa aatatattca aatggatgat ttggaaaaag tagacgaagt ttttaatatc
     3121 aacgaactta tagagtatct ttatcaaacg tggaagaaag acactgataa gcagcagctt
     3181 tttgagaaat atgatagata ttttagacaa aagaatgttg atatccaaaa taattcagct
     3241 ttaacatatc ccacctatca agagattcga tttagacagt atgttaagga aatagagaga
     3301 gattttcaaa tagaatttga ctattattat cttcaaataa cgtctatctt tttgaattct
     3361 tggatttatc aaatccaatt aacatcggac gataaattat attccttcat caatgatatt
     3421 aatagttggt gtagtcgaga agttcaatta gtcaatcgtt ttattcaaat aggggatatt
     3481 tatttaacaa aaattcaaaa aaatgttttg gttattttat ttaagttaat gtgtagtaaa
     3541 gaatttcagc aaaaacgttt agcactgatt ttggctcatg gagatctaac ggcaacctgt
     3601 atagcaagta ctgctaatag gatcgttgag agatatatat acgacgccat tgatatgcca
     3661 ctaatgactt caagtagtga gattgcagac aaaattaata gatacttaca agagtgtacc
     3721 ggatatgaag aaattatttt acttgttgat atgggttcac tggagaaaat ttatctaaat
     3781 ttggaactag acgctcaaaa aaaattcgga ctactaaaca atgtttcaac aagaatggct
     3841 attgatgttg caaatcagat attattagat ttaccgctta aacagattct tgaacaagta
     3901 aaagaaaatc aaaatgtgga ttatatttat gaatcatcaa atattaagaa ggatatgatt
     3961 gtctgttctt gtgcaagcgg attaggaact tcgtttaaat taaaaaagat acttgaagat
     4021 agctttccaa gggatactga tcttgtcata gaaacgtgtg actattactc tttagtgaat
     4081 gaaaactatt tatcggagct tcgtaaagag aacaatattc tttttattgt tggaacacta
     4141 aatccacatc tagaagatgt agaattcttt tcaattgaag atatgatttt aggaatgaat
     4201 ataagtaaat tctccaa
//
LOCUS       DAZULO010000152         4133 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000152 DAZULO010000000
VERSION     DAZULO010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.157"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..153)
                     /locus_tag="ACG8OA_002752"
     CDS             complement(<1..153)
                     /locus_tag="ACG8OA_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFS4977483.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            complement(475..696)
                     /locus_tag="ACG8OA_002753"
     CDS             complement(475..696)
                     /locus_tag="ACG8OA_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977484.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(794..1039)
                     /locus_tag="ACG8OA_002754"
     CDS             complement(794..1039)
                     /locus_tag="ACG8OA_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977485.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1279..1893
                     /locus_tag="ACG8OA_002755"
                     /pseudo
     CDS             1279..1893
                     /locus_tag="ACG8OA_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2263..3264)
                     /locus_tag="ACG8OA_002756"
     CDS             complement(2263..3264)
                     /locus_tag="ACG8OA_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFS4977486.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3423..3689)
                     /locus_tag="ACG8OA_002757"
     CDS             complement(3423..3689)
                     /locus_tag="ACG8OA_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4977487.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3679..4035)
                     /locus_tag="ACG8OA_002758"
     CDS             complement(3679..4035)
                     /locus_tag="ACG8OA_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4977488.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1309 a          749 c          548 g         1527 t
ORIGIN      
        1 tacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
       61 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
      121 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      181 aattttacac taaaatagat tttttggaaa actttgcaac agaaccgttt ttactatata
      241 cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag ttcattgggt
      301 ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt tatgtcctat
      361 ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt gtaatatgaa
      421 gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga accattatag
      481 atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa atgtttcaaa
      541 ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt aagtccaatc
      601 aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa ctgaagccaa
      661 ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc aagacgctac
      721 ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta tagcaaacaa
      781 atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg cttctattct
      841 taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac tcatttattg
      901 ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg gcatcctgat
      961 agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga acttctttta
     1021 caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat ctataatatt
     1081 tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta ttataaacta
     1141 tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa atagacaaaa
     1201 atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat aaacgttata
     1261 gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat cgatcaaaat
     1321 gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt tatcgaaaaa
     1381 caatctggag cgacgattag tcatcggccg attttccagg aagctttaga ttttgtcaga
     1441 gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta tgacgagatt
     1501 atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac cagtttaccg
     1561 atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga cttaatcatt
     1621 taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg ccaggcacaa
     1681 ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt atacagtgca
     1741 gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga tcttgagaat
     1801 ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac aatttaccga
     1861 ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac gtaattatta
     1921 attagctttg tcatcaatac agacaatcat tttagattca tctagctaag ttgattacat
     1981 ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg tccttattta
     2041 aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat aagatttgaa
     2101 ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat ttacgattgc
     2161 acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca ttaaaaaagt
     2221 ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg gtaaatctga
     2281 ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct ccataataac
     2341 agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt cctctgaaat
     2401 caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct ttaaaattcc
     2461 cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac tccttaaatt
     2521 gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg aaaagataga
     2581 attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa attctattgg
     2641 atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt ttttgttaat
     2701 tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt ctaaattatt
     2761 ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga tattgtcttt
     2821 gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct taacttctat
     2881 atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc ctacctcgcc
     2941 tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat aattatcaac
     3001 tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt ctacaatttg
     3061 tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg cattcgtttc
     3121 ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat tccatctata
     3181 tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg ctataaatac
     3241 ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc tttaaagtaa
     3301 tttttgtata aaatttattg acttttacac tataggacaa cttttatcta tttagtacaa
     3361 aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt ttcaactcgc
     3421 ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc atcaagttta
     3481 tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt tgtatgtgct
     3541 ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata aagagcaacc
     3601 gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt gtctttttgc
     3661 ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa gttcttttgc
     3721 atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat gggtaatgac
     3781 ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc ctaccttact
     3841 tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt gagctactag
     3901 catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat ctttttttgg
     3961 taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa atgtttcaaa
     4021 agatattttt tccataccca attatatcat atattgattt tatattatag tatataacat
     4081 aaaaattcga attatggttc tgttgcaaag ttttaaatct actatcaaat aag
//
LOCUS       DAZULO010000153         4036 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000153 DAZULO010000000
VERSION     DAZULO010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.158"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(242..391)
                     /locus_tag="ACG8OA_002759"
     CDS             complement(242..391)
                     /locus_tag="ACG8OA_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977489.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            853..1065
                     /locus_tag="ACG8OA_002760"
     CDS             853..1065
                     /locus_tag="ACG8OA_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977490.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1343..3142)
                     /locus_tag="ACG8OA_002761"
     CDS             complement(1343..3142)
                     /locus_tag="ACG8OA_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFS4977491.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3266..3862)
                     /locus_tag="ACG8OA_002762"
     CDS             complement(3266..3862)
                     /locus_tag="ACG8OA_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS4977492.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1449 a          711 c          689 g         1187 t
ORIGIN      
        1 atattaaata ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt
       61 cataaaaaac gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag
      121 agagacaatt tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt
      181 acaaacgtct tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc
      241 cttatctaga ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt
      301 tattttcgtt ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc
      361 ggtattgcat atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta
      421 gttcttgaaa gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt
      481 tgatgcaatt aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct
      541 attttctttt aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat
      601 ataaatcata tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga
      661 acaacatagg aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata
      721 gttatctaaa aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacgttt
      781 tcaaatacaa ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa
      841 agagggaatg aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga
      901 gtatctcatg aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat
      961 gttgaaagtt atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc
     1021 gtaaaattct gtgatattag cgcacctgct gaattggttt cttaaacagt atttttgaga
     1081 attcgcaaga actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa
     1141 aaagaaaaag ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta
     1201 ttttagctat tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg
     1261 tcaaacaaaa aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac
     1321 ttttttcatt aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg
     1381 aataagtgat ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg
     1441 ttatgatacc atccacacca taaaacatca tgcgactcat agcatctgaa tcgttgattg
     1501 tccatacata tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg
     1561 tactatattc cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag
     1621 gtaagatgta gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt
     1681 acgtcaaact ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa
     1741 atcgttcgac aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt
     1801 ggtttagctt atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc
     1861 cgttttcagt aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat
     1921 ttacccctgt taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg
     1981 tttcctgaac atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg
     2041 cttctaaagt attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg
     2101 gttttgtctg aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga
     2161 aaaaaactgc aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg
     2221 cttgtgggat aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa
     2281 caatgatata aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta
     2341 aaagcgtcat agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca
     2401 tactttgtgc caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc
     2461 caatcacaat aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt
     2521 cccgaatggc tgctcgaaaa ggacgatctc gtagaatcat ttccggtaaa gcaaaaatca
     2581 gtcgaatccc aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc
     2641 aacgattagc aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat
     2701 cagaattaaa acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca
     2761 aaaaagtaat gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca
     2821 aagagattgg ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga
     2881 aataaactaa cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat
     2941 gttgagaaaa aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta
     3001 aaataaaacg ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca
     3061 tcagcaagac atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta
     3121 agctacgttt caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat
     3181 gaaaaaggaa agggtgcagt gtgttttgca gaccctttcc cagatttaac attgtcaatc
     3241 aatgactaat tttacaaaat aaatattatc caagaagacc acttagatat tgacctccat
     3301 aaatcaaggc catactatat aagaaaatcg acagtggttt agcaatcaaa atgattagtg
     3361 tatatcgttt cactgacatg tttgtcaatc cagccatcaa acataacgca tcgtcaggag
     3421 caactggaag aaaaatagct agagcaaaca gcttctcaaa acggttttga ttatccagcc
     3481 agccaatata tttgttatat gtcttatcgc taatcatgga aagtatgaat ggctttccat
     3541 aacgacgccc tagcagaaaa atgataatcg aaccaatgca gattccaata tagttataga
     3601 taaatccagc ataagggcca aatatcagga caccagctgc agtactgatc cctccaggaa
     3661 tgattggaat aactacttgc aggatttgga tgaagacaaa aataatcggt cctaaaatga
     3721 tggaatcacc tactaatcct cttaacgcat tcaaatcttt gaaaacacct aaattaataa
     3781 aataaatcgt cagcgcgatt gataggccta atccgataat ggatatgaaa tttatcagtt
     3841 ttcgagaggc agcaatattc attattcatc aactccttag aggttctttg ttaactatac
     3901 tttactagaa aagcaactaa ctgcgccatt ttttgatatg acagaaaaca aacaattttg
     3961 taagttcatc ataagtataa aagattgatc tcaaaaaaac atccaactaa agacttattt
     4021 aataacttgt tttttt
//
LOCUS       DAZULO010000154         4034 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000154 DAZULO010000000
VERSION     DAZULO010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.159"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..234
                     /locus_tag="ACG8OA_002763"
     CDS             73..234
                     /locus_tag="ACG8OA_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFS4977493.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="ACG8OA_002764"
     CDS             253..1530
                     /locus_tag="ACG8OA_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4977494.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="ACG8OA_002765"
     CDS             1540..2196
                     /locus_tag="ACG8OA_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4977495.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="ACG8OA_002766"
     CDS             2196..3572
                     /locus_tag="ACG8OA_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4977496.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3702..>4034
                     /locus_tag="ACG8OA_002767"
     CDS             3702..>4034
                     /locus_tag="ACG8OA_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS4977497.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1612 a          440 c          704 g         1278 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa
     3721 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     3781 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     3841 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     3901 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3961 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     4021 atgtctccga catc
//
LOCUS       DAZULO010000155         4029 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000155 DAZULO010000000
VERSION     DAZULO010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.160"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(679..1236)
                     /locus_tag="ACG8OA_002768"
     CDS             complement(679..1236)
                     /locus_tag="ACG8OA_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977498.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1415..2026)
                     /locus_tag="ACG8OA_002769"
     CDS             complement(1415..2026)
                     /locus_tag="ACG8OA_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977499.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2139..2396)
                     /locus_tag="ACG8OA_002770"
     CDS             complement(2139..2396)
                     /locus_tag="ACG8OA_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977500.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2993..3271)
                     /locus_tag="ACG8OA_002771"
     CDS             complement(2993..3271)
                     /locus_tag="ACG8OA_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977501.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3305..3556)
                     /locus_tag="ACG8OA_002772"
     CDS             complement(3305..3556)
                     /locus_tag="ACG8OA_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977502.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1226 a          827 c          538 g         1438 t
ORIGIN      
        1 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc
       61 aactccttct tatttcttgg agttaaacgc ttctgtccca gcctccttat tccgaataaa
      121 tggtgtccaa aagcttgcat ctgccagatt atctcatttc ttatgacaag ctaagcaaat
      181 tatctccacc aaataattta ttcatatttt tgacttgcgc atactaacta attcttaaat
      241 tttcatacaa aatttctcaa tttcccttaa cgagtaatat ttatgtacgg tgattctcac
      301 tagtatccca atcacttttt aagttatccc gatttcaatt ctacttcaaa aatagttgct
      361 tttatgtatg ctgttagttg attagatggt catttgttga gctaggtcgt ttattttaaa
      421 caagttgttt ctttagctta tacctatctc gtctaccaaa cgcaagatgg caaacacaaa
      481 tgcatacgca tctgtatttg ccactttgat tcgtataaag ctaacaacta ttaactcctc
      541 tcagctaata gttgctctct tttttattca gattcatttt tgctgatcac agcctagtat
      601 ttcaatggtt ttgaacacta aaccaaattg ttccattaca acattcactg gctgatgaaa
      661 tggattagta gatttcgatt attcaaagcg attttctatt ggtggatcgt gttcccaact
      721 aataagacgg attccaaacc tatcgtacag atcttctaaa ccacttataa attttgcacc
      781 tatttcaggt gttttattgc gtggatattc tattgttacg ccattaaatg gataccaatt
      841 aggattaggc tctctttttt tctgtacatc tcccatagaa tagttagcac tttctgggac
      901 ataatatatt cttcgaccat cctctaattc atctaataag atatttcgtg atcgtctatc
      961 acttgctaaa tgtagacgcc acgtgcgata ccatgccatg ctacctgcat ctgcagaaaa
     1021 tccatcaaat aatgcaacaa ttgtatcttc tatcacaatc ctttccccat ctacttcata
     1081 ttctaatcta aaatcaaact cccctcgggt aatttctggt cttggtggct taggacccaa
     1141 tattattcta cttaaaaccg cattcgttcc cagatgaaac acagctataa caacaataat
     1201 gattaatatt ttgtttctat ttgataaatt tctcattatt cgcacatcac ctctacattc
     1261 tcacatatta gttcgtaata ctcctttaat atcaaaatat ctactggtcg taataatatt
     1321 cgctatgaat tcctactact cgtataagaa aggccaaatc atcagctttt acttgctgat
     1381 gatttggctt gtttctattt atcgtaattg ttttctaatc accaaaattg ttaacaatcg
     1441 gttcagatat ttcccagttg attaaggtaa ttccgtattg tgtcaaaact tcttgcgctt
     1501 ctccgaaatt aagtacagtt cttatgtatc cactttctcc taatataaag tctacctctc
     1561 ttttaaaacc ttcctcgaga tttctaaggg ctatatgtgg ttttaatgac attgattgta
     1621 cattttcccc catataatat tgcgcacttc ctcgactaaa atagattcct agatcgtccc
     1681 caaaaggtag atcttctcta ccactagcta atctacttct ccatcttcgt tctcttttca
     1741 tagaacctgc gctgaactca cacccgttaa actcagctat gacagtatct tcaattatgt
     1801 taatctgtcc atctagttcg tattcaatcc taaatggaaa ttctccatag gtgatttccg
     1861 gttcgggtat tttaggtgag attaatgcaa agaaaggaaa tattaagctt ggcaacatca
     1921 aaaaaactcc tcctatgatc acaattcccg atgtaatcca aaatataatt tgtacaattc
     1981 tcattgatcc attgtattcc attttattcc ccctattact attcatatcc gcctatctat
     2041 atcgatcttc caatgcagag ataattgctt ccataccttg attttttact gagttaccta
     2101 tgcgagatat cattctccac taaggtgcat catattgtct atctaacatg attaattctc
     2161 caataggtgt atactcctcc aaaaacaaca tttaatgcat ctccctcgac aacataagct
     2221 accatgtttg ctgtatatgt gtttgagtct aattcataag cagataaatt tactgtcgct
     2281 ggattaaata taatcgcatt cgtatttata gctactgaat ttgctgcttc agcaccacct
     2341 tttgaatgtc cgatcattgt gatttcaata gtaggattta cgtggacagc cctcaccgtt
     2401 taccctgacc attatagaaa tcgcttactt ttttgaacta cgcaacaaag gatacaatgc
     2461 tcctaacttc actgtctcca tttttgtcca ctaaggaata taaaaaatag gcattaattc
     2521 atttaattcg gtcacaacac gttacctttt aaatcattat actgtatctc atacattatt
     2581 atcaataaac tattataaaa atgcaaaaaa tgctttatca atctattttg taattggtca
     2641 taatatcatt gaaatcatac cctgtatcga tttttcctat gaatctcgtc tatttgtgat
     2701 tgacgaaaaa gaggcaagac agacactgga atggtaattt tacccactat tttgctccaa
     2761 ctatttgtaa tcgtacccac ttttgaaata ccgagatgat cattttaatt gatatgctga
     2821 ttcccaagca aaaccccaag aaattaaatg ctctatcgca tctagcttct tgggttttgc
     2881 ttatataaat tccaacattt aaatttttta tgctgttcgg tctcagaaat gccgtttttg
     2941 cttatatcac aatatcaaga tgtcaaatta ttcgtgttgc cgatgacttt gattaatttt
     3001 tttcaaattg tttattaaga ctaatgctgt ctttttgcag ataccaatgt tcgaactttt
     3061 taacattatc atatgatcct gcaagtttta acttcttacc attagatgtt atcaaagtta
     3121 taattaacct tgcctttttc ccattaggat cttttatttt atgaattttc tgcaggtaat
     3181 ctctcctaat ggcagctatt tttacctcat cagtgctgat aaaccattga tcatccacat
     3241 acatactgcc tgttatgttg tgtttttcca tttcttccga gaacgaaaag tttaaggctc
     3301 gttcttactt ccaaagttga atgagtccat ggattggact tagtgtgaga attgagaaca
     3361 acaataagaa tccatatcct cccgcaaaca tccctaaggc tatactgtct ctccatccat
     3421 aagtaggatt agaagagaaa agagtcaccg tttgtaataa tatgaaaagg aataatccag
     3481 cacaaacacc aaagataaca actccaaaca ttgaattttt atacaatgac catttattta
     3541 taggcttttc tttcatttta cttcccccta ctgttctgtt cacacggaat gaattcaaag
     3601 ttcataagcc ctcctctttg aacatatgaa tacgacttgc taactatact ttaaaaaagc
     3661 ttgttttaag cggttatagg cgagttgtaa tctctattca tctcattagc aaataacttg
     3721 ccgaaaagtc tactgaaatt aaagcagata gtggaagaat tatttagcca ggatagacac
     3781 tatttgtgaa acacatgtct ttacttttga aattctatga tgaaaagcct tggaatacag
     3841 tagctcttaa ttggtaatac tttttccatt ataaataacc tcttttcatt ttatatttat
     3901 cccctctaaa aataatatct aatctaatat tttaaactta tatttccctc gctgtgctaa
     3961 tagagcatta ctaaataaat agaaactaga aaaaagcatt acgcctgcca gaataaacca
     4021 attatttgt
//
LOCUS       DAZULO010000156         3986 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000156 DAZULO010000000
VERSION     DAZULO010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.161"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1026
                     /locus_tag="ACG8OA_002773"
     CDS             <1..1026
                     /locus_tag="ACG8OA_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HFS4977503.1"
                     /translation="NTIFSDEYVEIIISDHSKNTKEIAKKIEEKKVFDGCHYLESKSF
                     DDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFDEFICFSMGEVWLEGIYACLYR
                     NNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLKKRTPLYNKSYNFYCFFPNIYT
                     GRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKYIFFTSVYDFEGGEPVGEYELV
                     CKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSSIPWEAIQLSGDFNDKIFMTIN
                     SGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAYDIERLLKQKTMKNILKNVKIA
                     ESLDDIL"
     gene            1023..2444
                     /locus_tag="ACG8OA_002774"
     CDS             1023..2444
                     /locus_tag="ACG8OA_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS4977504.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINIS
                     YIKQLCYRGRNIISMIKSREHKR"
     gene            2480..3196
                     /locus_tag="ACG8OA_002775"
     CDS             2480..3196
                     /locus_tag="ACG8OA_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFS4977505.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            3215..>3986
                     /locus_tag="ACG8OA_002776"
     CDS             3215..>3986
                     /locus_tag="ACG8OA_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HFS4977506.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLL"
BASE COUNT         1475 a          465 c          724 g         1322 t
ORIGIN      
        1 aatacaattt tttccgatga gtatgttgaa attattattt ctgatcattc taaaaacact
       61 aaagaaattg cgaaaaaaat agaagaaaaa aaagtgtttg atggctgtca ttatttagaa
      121 agcaaatctt ttgatgataa tattgggtat tttcaaaaga ttaaggactg tcttcagatt
      181 tcttgtggta aatcaaatcg atatgcctat tatttgaaaa actgtaaaag tgaatatttt
      241 gatgaattta tttgtttttc aatgggagag gtttggttgg aaggaatata tgcttgctta
      301 tatcgaaata ataaaaatgt acaggtatca attttagaag aaggtatttt aagctatgga
      361 gttgacatta ttccaacagc aagaacaaaa gctatcgcag tactcaatta cttaaaaaaa
      421 aggactccac tatataacaa aagctataat ttttattgtt tttttcctaa tatatataca
      481 ggaagagaga atacaataca aattccatta gttacagctg ggtcaagaac cgcattgcaa
      541 ctttcggata tttttaaccc tatgatagat atttataaag aaaaaaaata tatatttttt
      601 acgtctgttt atgattttga gggcggagaa cctgtcggag aatatgaatt agtatgcaaa
      661 atagcagact tagtaggaaa agataatcta attgttaaga cgcatcctag agatgtaaga
      721 acaatttata aagataatgg atttatcgag gataaaaatt catcaatacc ttgggaggcg
      781 attcaattgt caggcgattt taacgataaa atatttatga caattaattc aggaagtgtt
      841 atttcaggta gtacaatgtc tgcaaaacct gtacgcacgt attatatgta taaattgtgt
      901 aacatagaag gaaatccgtc ttgcaaaaag aatgcgtatg atattgagcg attgcttaag
      961 caaaaaacga tgaaaaacat tttaaagaat gtgaaaatag cagaaagctt agatgatata
     1021 ctatgagcaa aactaaatat ttaattaaaa atatgggaat attaacaata agtaattttt
     1081 cttcaaaaat tttagttttt ttgttagtcc ctttatatac aagtgtatta tccacaacag
     1141 aatatggcac atatgatttg acaatttcaa cagccacatt attgtttcca attttaacct
     1201 taaacattgt agatgctgtg atgcgctttt taatggataa agataaaaat aaaaaaaata
     1261 tagcatctat agcgattaaa gttatatttt taagcgtgat agtctttgga tccgtaatgt
     1321 atttgttaaa tattacaaaa ttatggcaag atgtttatgg attagaaaaa tatatttttt
     1381 tgtattatat atcctatgta tggaatcaat ttctaataca atttgctaaa gggttagaaa
     1441 aagttaaagc tatgggcata gcaggtatta tcagcacaat tgtaacaata attgccaata
     1501 ttttcttctt gcttattctg aaatggggat taattggttt tttcattgca aatattttat
     1561 cacagattat ttcagctact tatctgtcag tattagtgag aatatgggaa tatatcgatt
     1621 ttgaggctaa agatcctcag ctaaagagag aaatgctaaa ttactcgatt ccattaattg
     1681 caactgttgt tggatggtgg attaatagta cagctgatag gtatgtggtt tcctttatag
     1741 taggtgtctc ggcgaatggc ttattgtctg tggcatataa aatacctcaa ataattaata
     1801 ctttgcaggg aatatttata caggcctggc aaatttctgc aattaaagaa tatggagata
     1861 atgagactgc aaaattttat ggaaatacat ttgttatgat taatttgtta atgtgcataa
     1921 cctgttcaag tcttatcata ttaactagac cgttttcaca tattctttat gcgaaagact
     1981 tttatgaagc gtggaaatat gttccatttt tattaatagc taatgtttta aattgtgcat
     2041 caggtctgtt aggacctatc ttatcagcaa aaaaagattc taaatccatg atgtggtctg
     2101 caatcatagg ggcattagtt aatgtgatta tgagtgtagt ccttgtttat aagatagggg
     2161 ttcaaggtgc cacaatagcc actgttatat gttcatatgt aatatatgtt gttcgtaaac
     2221 gagctgtggg aaaagatatt tttataaagc attatcatat tgttgtggaa tcttggggat
     2281 tattgtttgt tcaggcaatc atagaaatat attttaaaaa ttatgtaata gaaattataa
     2341 ttatgattat tttaattatt ataaatatca gttatattaa gcagttgtgt tatagaggaa
     2401 gaaacattat ttcaatgatt aagagtcgag agcacaaacg gtagacgatg ttatttttat
     2461 aaaaagatat aatttattaa tggtcgataa ttatgaaagg aaaaatattc ttaaaaaaaa
     2521 gagtatagga tgtcgtatga aaatagtcgc tatagtacca atgaagttaa ataacaaaag
     2581 attgccacaa aaaaacacga aagcttttac caatggaaaa ccattgtgtt attatatact
     2641 ttcaacactt ttaaaagtaa aacagattga taaagtgtat gtctattgta gtaacccaga
     2701 tattaaagaa tttataccag aaggtgtaac atttttaaag agatctagtt ccttagatca
     2761 ggatacaacg agtattacac aggtattagc cggtttcaca aaagaagtag aggctgatgt
     2821 atatgtaatg acacacacaa cagcaccatt tattagcgaa aaatctattg aaaaaggact
     2881 agatgccgtg ctgacaggag aatatgattc tgcatttgct gcaaaaaaat tgcaagattt
     2941 cttatggaaa gacggtgaac catttaatta tgaattggac aatattccta gaacacagga
     3001 tttgccgcct ttatacgaag aaactagtgg attttatatt tataatcgca atgtgatgac
     3061 cgagttgcat agaagaattg ggaaaaaacc ttgcatagtt gaagtaggtg agattgagtc
     3121 agttgatatt gatgaagccg aggattttat gattgctgat gcgatttata atcatcttat
     3181 tttacaaggt agttaagaca gggggaaggt taggatgaac aatgtacaga ttttagattg
     3241 cacacttaga gatgggggct attgtaacaa ttgggaattt ggatttgata atacaaaaaa
     3301 aattatttct ggacttatag aatcaggaat tgagattata gaatgtgggt ttctcacaaa
     3361 taaaacagta tacaataaaa atgttacaaa atatacgacc ttagaagaat tagcacagat
     3421 aattcctgaa aaaagagaag gaaaattatt tgtagcaatg atgaactatg gcgaatataa
     3481 tatagaggat cttccagatt atgatggttc atctattgat ggaattagga ttgcgtttca
     3541 taaaaaaaat tgcaatgaag cgttgaaatt atgtcgtgag ataaagaaaa aaggctactt
     3601 agtttttgtt caagctatgg tatctctcaa ttatacagat gaagagtttt tagcagtaat
     3661 aaaaaatgtg aatcagttta aaccatacgc tttttatatt gttgatagtt ttggaatgat
     3721 gaaaggaaaa gatttaactc gtctttttta catgattgag cataatctag aaaaagatat
     3781 acagataggt tttcactcac ataataacat gcagcttgca tattctaatg cacaaaaact
     3841 tataaccgta caaacaaatc gaagcctgat tattgattct tcggtgtacg gaatgggaag
     3901 aggagcaggt aatcttaaca cagaactatt tgtagaatat ttgaatgata atgcaggaaa
     3961 aaaatatcaa ttaaaaccat tattat
//
LOCUS       DAZULO010000157         3885 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000157 DAZULO010000000
VERSION     DAZULO010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.162"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..923
                     /locus_tag="ACG8OA_002777"
     CDS             33..923
                     /locus_tag="ACG8OA_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFS4977507.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(996..1820)
                     /gene="nadE"
                     /locus_tag="ACG8OA_002778"
     CDS             complement(996..1820)
                     /gene="nadE"
                     /locus_tag="ACG8OA_002778"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFS4977508.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1832..3304)
                     /locus_tag="ACG8OA_002779"
     CDS             complement(1832..3304)
                     /locus_tag="ACG8OA_002779"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFS4977509.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT         1140 a          795 c          685 g         1265 t
ORIGIN      
        1 atataagaaa aaccttttaa gaggtgatat atatgcaact acaaataaac gaagaatcct
       61 taccggtata tgaagcatta gctagtaaaa caaggatcaa gatcatccag ctactttcca
      121 aaaaaaagat gaatgtcaaa gatttggcaa aagaacttgg agtgagcagt gctatcacga
      181 cgatgcacgt gaaaaagcta gaggaagcaa acatcatcaa aacagaaaaa gtaggacaac
      241 aaaaaatttc gagccttcgt gtcgataaaa tcgatattag ttttccagaa aaaatattca
      301 atgcttttga tacgaaagaa acatcgatac ctattggtca ctatacaaat tatgcaattg
      361 aaccaacatg cggtttagct acgatccatg attttatcgg gaaagtggat gaaccacgct
      421 attttatgga tcctcgaagg atggatgccc gcattctctg gtttaccagc ggatttgtcg
      481 agtatcaggc acctaatttt ttaaatacag aggatacatt ggagatgttg gaagtttctg
      541 tggaaatttc ttctgaattt cctttttcaa atgacaattg gccttcagat atcactttca
      601 gcctaaatgg tgtggagcta ggaacatgga cgagccctgg agattttgct gatatccgcg
      661 gtaaatatac gcctgattgg tatccagata atcttaatca atacggcttg ctgaagacga
      721 tccgaattac caaacacttg acgaatatga atggagaacc cttgtcaaac atcacgatca
      781 acgacatccc taaagagcaa gacacttggc atttgcgaat cgaagtgaaa gacgatgcca
      841 aacatgtggg gggctgtact ttatttggta aaggattcgg taattacgat caggatatca
      901 aattaaaagt ttactacagc tgatattttt cttttctatt ttataaaaca aaaacagcca
      961 atccctatga agggacggct gtttttgctt tattgttatt tccagaaatc atccaaaacg
     1021 gaaattggca aatgtcgttt atgttgtgtc ttgttccacc atgcttcgat tttttcttgt
     1081 gcttgagcgg gaacttcttt tccttctaga taatcatcaa tttcattata tgtgacacct
     1141 agtgcgactt catctgcaat cattggtttc ccatcttcaa gatctgcagt cggaattttt
     1201 gtgtataatt tttccggtgc attcaatgct tgaagcaatt gtttcccttg tcgtttgtcc
     1261 aaacggaata atggcaaaat gtcggctcca ccatcaccaa atttcgtgaa gaatccagta
     1321 atgttttctg cagcatgatc tgtccctaat acggctcctg ctctttctcc agctacggca
     1381 tattgcgtaa tcatacgttg acgtgctttg atatttcctt tgttaaaatc gctgattccc
     1441 acacctgctt cgcttaatac ttgtacttgt gcatcaacag ctggtttgat atttactcgc
     1501 agactaacat cgggctggat aaagtcaaga gcagcttttg catcttcttc atctgcttgt
     1561 tcaccataag gaagacgaac agcgataaat tgatagtttt catcttttgt ttcttcacgc
     1621 atttcttcca tcgttagttg cgccaaacgg cctgctaaag tagaatcttg gccgccgctg
     1681 atacctagta caaaagtttt aagaaacgga tattttttca aatatgcttt cataaagtca
     1741 atacttttac gtatttcttc ttttggatca attgttggtt tgacacctaa ttcattgata
     1801 atttctgtct gcaggctcat ttattctcct cctatgcttc tgtatgaagt tctgctacgc
     1861 tttttttcat tcgttccatg atagccattt tatggttcca gcattcagta gacagatcga
     1921 ctgggtattt ttgcggattc aagtctcgtt tatactcttc ccatagtgaa tctagatttt
     1981 cttttgtata ttctttgatt tcgtttagtg caggtaattc gtaaactctt tttccttcga
     2041 taaagatatc ttgtaaaact gggcgagctg taaagtctcg aacagtttta ttgataaacg
     2101 tatgcactgg atggaacatg aagatagctt cttcttctcg agggtcttca tcccataatg
     2161 tcacgtagtc tccttcagat ttcccatcaa aatttcgagt gatgcgccac acttgttttt
     2221 tccctggagt agttactttt tctgcattgc ttgataactt gatcgtatcc atcatttttc
     2281 cttcatcatc ttcgatcgat actagcttga atactgcacc aagggctggc tgatcatagg
     2341 ctgtgatcaa ttttgtccca acaccccata catcaatttt agctttttgc atctttaaat
     2401 tcaaaatcgt tgcttcatcc aagtcattcg aagcatagat ttttgcttcc gtaaatccgg
     2461 ctgcatctaa ttgttcccga acacgcttag atatataggc catatctcca ctgtcgatgc
     2521 gtacgccttg gaagttgatt ttgtcaccta attctttcgc cacgcgaatc gcacttggca
     2581 cacctgattt caacgtatca taagtgtcga ctaagaacac gcagtcttta tgggttttag
     2641 cgtaggccat aaatgcttca taatcatttc cataagattg gactaacgaa tgagcatggg
     2701 tcccgctagc tggtatccca aatatcttcc cagcacgtac attgcttgtt gcatctgctc
     2761 cgccgatata tgccgctcta gttccccaga ctgctgcatc cagttcttgt gcccgtctag
     2821 ttccaaattc caatagtgga tcttctccga tgacagattt gatacgagct gcttttgtcg
     2881 cgatcaacgt ttggaaattg accatattca acagtgctgt ttctactaat tggcagtgcg
     2941 ctaaaggccc ttccacttgg atcaatggtt catttgcaaa aactagttct ccttcacgag
     3001 cagaacgaac tgttgcttta aattcaaaat tttgtaaata ctccaaaaaa ccttcaggat
     3061 agacttctaa tccacgcaag taagcaatat cggaatcatt gaacgtcaga ttctctaaat
     3121 aattgactaa ccgttccaat cctgcaaaaa ccgcgtatcc gtggttaaaa ggcatttctc
     3181 gaaagtaaca ttcaaaaaca gcatgtaaat cagctcgacc taattcccag tatgtctgca
     3241 tcatattgat ttgatataga tccgtatgca gcgtcaaact gtcgtctggg taagtattat
     3301 tcatcttgta ttactcctct tggcaagttt tcatttccta cccattataa caaaaaaaat
     3361 taataggaaa agaaatcttc cattttttct gaaaaagcac atgttttacg gatatttctt
     3421 gctaaaaatg aactccatct gctaaaacta tcctttctta ttggtattta acctaaacga
     3481 gaaagaatac ttattttatt caatgtatcc gagacacgaa gaaattagcg ttttctattt
     3541 ttcttacttg ataaaataag tagattttcc tactacaatc gtagccgtta agataccgat
     3601 gattgaacaa atataaccaa atgtctctac aacataatca atggcaaaag tttttgaagc
     3661 aataatacgt ctttcctttt ttgcttctta tccccaaaga aacggatata acttattatg
     3721 tattgaattt ttttggaaag gcgaattatg acaagatata ccttgcgttg aaaaaactcg
     3781 cggaaaaaat atcacacgtt tttaaaaaga tacaaagtaa gaaagcagct gtcaatagaa
     3841 tggaaacaaa aatggcgaga catcaatcag tctcgccttg ttttt
//
LOCUS       DAZULO010000158         3876 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000158 DAZULO010000000
VERSION     DAZULO010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.163"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..2257
                     /locus_tag="ACG8OA_002780"
     CDS             158..2257
                     /locus_tag="ACG8OA_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFS4977510.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2453..3316
                     /locus_tag="ACG8OA_002781"
     CDS             2453..3316
                     /locus_tag="ACG8OA_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFS4977511.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3421..>3876
                     /locus_tag="ACG8OA_002782"
     CDS             3421..>3876
                     /locus_tag="ACG8OA_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS4977512.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT         1544 a          646 c          782 g          904 t
ORIGIN      
        1 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
       61 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
      121 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      181 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      241 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      301 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      361 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      421 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      481 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      541 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      601 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      661 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      721 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      781 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      841 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      901 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      961 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
     1021 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1081 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1141 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1201 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1261 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1321 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1381 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1441 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1501 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1561 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1621 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1681 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1741 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1801 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1861 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1921 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1981 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     2041 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2101 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2161 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2221 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2281 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2341 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2401 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2461 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2521 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2581 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2641 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2701 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2761 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2821 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2881 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
     2941 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
     3001 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
     3061 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
     3121 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
     3181 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
     3241 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
     3301 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
     3361 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
     3421 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
     3481 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
     3541 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
     3601 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
     3661 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
     3721 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
     3781 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
     3841 catggaatct gtcaggaatg ctatgaaaaa gaaaaa
//
LOCUS       DAZULO010000159         3864 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000159 DAZULO010000000
VERSION     DAZULO010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.164"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..135
                     /locus_tag="ACG8OA_002783"
     CDS             <1..135
                     /locus_tag="ACG8OA_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4977513.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(313..2310)
                     /gene="tkt"
                     /locus_tag="ACG8OA_002784"
     CDS             complement(313..2310)
                     /gene="tkt"
                     /locus_tag="ACG8OA_002784"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFS4977514.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2412..2663)
                     /locus_tag="ACG8OA_002785"
     CDS             complement(2412..2663)
                     /locus_tag="ACG8OA_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFS4977515.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2810..3433
                     /gene="lexA"
                     /locus_tag="ACG8OA_002786"
     CDS             2810..3433
                     /gene="lexA"
                     /locus_tag="ACG8OA_002786"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFS4977516.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3613..3831
                     /locus_tag="ACG8OA_002787"
     CDS             3613..3831
                     /locus_tag="ACG8OA_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977517.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1080 a          827 c          707 g         1250 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
      181 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      241 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      301 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      361 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      421 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      481 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      541 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      601 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      661 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      721 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      781 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      841 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      901 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      961 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
     1021 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
     1081 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1141 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1201 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1261 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1321 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1381 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1441 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1501 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1561 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1621 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1681 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1741 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1801 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1861 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1921 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1981 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     2041 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     2101 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2161 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2221 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2281 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2341 ttacctttat ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc
     2401 agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct
     2461 tttaattttt ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata
     2521 tgatgacgca taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct
     2581 tgttcttcgg cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc
     2641 tctatttttt caggtgacag catgctcagc cctcctttta ctctccttca tattatataa
     2701 aaactgaaaa aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc
     2761 gctttttata tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca
     2821 aactgaatca agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg
     2881 atacccgccg actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt
     2941 tcacggtcat cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc
     3001 tcgtgcaatc gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat
     3061 tccagtactt ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga
     3121 cttctttcca atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat
     3181 tcgtggagat agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa
     3241 gcaagaaact gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac
     3301 ttgcaaacga ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct
     3361 cgaaccaatc atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag
     3421 tcatatctat taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg
     3481 gtctcaaact cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt
     3541 tacaaaccga acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag
     3601 aggtgagaat tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta
     3661 ggtattatta ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt
     3721 actttgtggt tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt
     3781 catgaagatc atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg
     3841 actgctaatt attcaaaaga caag
//
LOCUS       DAZULO010000160         3679 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000160 DAZULO010000000
VERSION     DAZULO010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.165"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..545
                     /locus_tag="ACG8OA_002788"
     CDS             75..545
                     /locus_tag="ACG8OA_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFS4977518.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGFPYFDFGWHIFHAKFCDFNSIGKI"
     gene            complement(465..683)
                     /locus_tag="ACG8OA_002789"
     CDS             complement(465..683)
                     /locus_tag="ACG8OA_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977519.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            790..1104
                     /locus_tag="ACG8OA_002790"
     CDS             790..1104
                     /locus_tag="ACG8OA_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4977520.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="ACG8OA_002791"
     CDS             1208..2386
                     /locus_tag="ACG8OA_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4977521.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2567..2752)
                     /locus_tag="ACG8OA_002792"
     CDS             complement(2567..2752)
                     /locus_tag="ACG8OA_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977522.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            2789..3082
                     /locus_tag="ACG8OA_002793"
     CDS             2789..3082
                     /locus_tag="ACG8OA_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFS4977523.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(3170..3316)
                     /locus_tag="ACG8OA_002794"
                     /pseudo
     CDS             complement(3170..3316)
                     /locus_tag="ACG8OA_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT         1256 a          542 c          715 g         1166 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     2581 ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt ggtaggggag
     2641 aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc actatgataa
     2701 tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac ataaatggag
     2761 tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc aagaaaagat
     2821 gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa
     2881 aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag tgacttatac
     2941 agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt atgtcgaaca
     3001 aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc gggcatatca
     3061 gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa gttcaagtat
     3121 gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc tataatacaa
     3181 agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag cgattcgcag
     3241 ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa tcaattggat
     3301 gcgttacatg aataaagata attctctttt atttgtacga ataaatacca aaacgtacct
     3361 cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc gttcttgaaa
     3421 caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc tgagcgttag
     3481 aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga atacaagatc
     3541 agctaattga acaccgttta ttcgtgatga gggacacaac actgcagtcg acacatttct
     3601 cttttatgtt caaagataac aaatatttaa aacaaagaaa aatagtagat aatcccgtcc
     3661 taactctttt attcttccc
//
LOCUS       DAZULO010000161         3678 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000161 DAZULO010000000
VERSION     DAZULO010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.166"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            428..1468
                     /locus_tag="ACG8OA_002795"
     CDS             428..1468
                     /locus_tag="ACG8OA_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS4977524.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            2292..2624
                     /gene="traE"
                     /locus_tag="ACG8OA_002796"
     CDS             2292..2624
                     /gene="traE"
                     /locus_tag="ACG8OA_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFS4977525.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3243..3584
                     /locus_tag="ACG8OA_002797"
     CDS             3243..3584
                     /locus_tag="ACG8OA_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFS4977526.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1355 a          482 c          679 g         1162 t
ORIGIN      
        1 ttgtattttt tagtagtata taatctattg ttttctagcc taaagccttg tcttataaga
       61 ataatataat atatactact gaaataaggt agtatatatt actagtaatg gtagtatgtt
      121 ttatatgcat aaggtagttc ttaatttatt ttttgttttg acactggtag tacttaatta
      181 tctttaattt taaaactaaa ttttattcat acgtttttct ggtagtattt aattataaat
      241 aatatttatt taaagtagtt cttaatgact ttaaaggtag tacataattc attttatttc
      301 tgattttagg tagtatttaa ttaaaaaaca gttttaatgc gtttgcattt tttatttatc
      361 tttgctattt tcgaaaagag taggtaattt tacataaata aaacaacgtg aaagtagagg
      421 atctacaatg actgatttta aattttttaa ggcggataga gtttacaacg aattatttta
      481 tcaatttcca aaagtcttta ttgtttctga cgaatacaaa aaaatgaaag attcaactaa
      541 gattgcctat atgcttttaa aagcaagatt agagatcgca atcagcaaac ggcaaatcga
      601 tgaagaaggt aatgtttatt ttacttatac gacaaatgaa ctatgtagag tattaaactg
      661 ccaaaaacaa aaagcgatag caatcaaaaa agagttggaa tcctttggtt tattattaca
      721 aaagcagatg ggatttaaca aacagttagg gaaaaataat cctaatagac tatatctagc
      781 agaattaaaa gtctcagaaa atgatatcta cttactcgaa aaatttgata gagagaatag
      841 ggaaaacgtt gataaatcag agggtatgaa aatcataccc accctcgacg aaaaatcaga
      901 cgctgaatcc cttggggctc aagagggtat gaaaatcata ccgtgccaaa acgttgataa
      961 atcagagggt atgaaaatca taccagaact taataataat atattagaca ctaatagaca
     1021 caatatagac actgaaaaag accgcctaca agatcaattg ttgttagaca attttgagac
     1081 aattatgaca aacgacagca ttgctacgtt tgtccctgaa cgagtattaa atttgataaa
     1141 aacattttct tcaagttaca gtgaagctca aaaaaccgtc cagactattc ataatgcaaa
     1201 gaaaaaagct gaaatagaaa gtgatatttc gatagttttt gaagaactcg atagttatta
     1261 tgtcaatgca gaacaagaat tatacacgac actgttaaaa gcctatcaaa aattaaaaac
     1321 cgaaaaagtc gaaaatatcc agaacctgat ttttgtctat gtaaaaaatt ggtttatcga
     1381 aaaaccaata gctgctaaag tatcaagtga aaaacgtttg aattatgcaa gctccccaag
     1441 cactattacg aaagactggt tagagtgaaa ctttactgac aaagtgattg ttgcttcatg
     1501 gagtaacatt aaaattaaaa attttttatt attctaatta tattacgaat attacaaaat
     1561 aatagctatt ttaatagaat ttataagtta aaatattatg ataaaaatca caaacgtagc
     1621 cttttacgtc ataagtgtta aaagaaataa ttaatttcaa atatagaaaa gggaacgtat
     1681 gtttgcatac tccttttttt tgagttataa taatacgcga agagagataa tttcattatt
     1741 taaacaagct aaaaatcttg atattacttc ttttcaggca atacgaatat tattcgctta
     1801 cattttttgt ataaatttaa ttgtctgcta tgcttgtgat gaattgattt tcgttttgca
     1861 cttttggatg ataaatttgg acaattgaaa atacaaaaaa aagaaaggcg gtatgaactc
     1921 ggaaggctgg taaccttcgt cagcgttcat acacggcatt aagatttggc ccttaatgcg
     1981 ttaacctttc agtgttacgg tattacccgc aatggattta gtataacaga tattcaaaag
     2041 tgataccact ttttttggct gtgttaaggt taaaaatacg tgatattacg atgaatctat
     2101 gcctaagcat aatgttataa aaatgtccgt gtggctcttt gccgtatgac gaacgctgaa
     2161 attagaggga acgcatgttt ggatgcgttc cctctttttt gtttgtcatg aacgaaaatc
     2221 aattctctat ttttgaaaaa taacaagaaa aacttgttaa aaatatttaa agaaattggg
     2281 tggtttattt tatgtcatat acaggaattt tgagtctcaa agatatttgt cactacggta
     2341 aacggtgtac cgctacagag aaaatcacga agaaactatc aactggccaa agtaaaaccg
     2401 ttgtgcagtg taagaaatat ataatccaga aagacaaagt ctcagaggaa atgatttatt
     2461 acattgggaa acaaaagcaa ataattttaa aagatcctat ccccttaaaa gagctatatc
     2521 caacaatcaa acatgtttat gaccaaaacg gtgtactgat tggtcgaaga aaaaatggag
     2581 tcttgcgttg taccgcaaaa gggatggggc gtttgatcgg ctgaattgat cattctcata
     2641 acttgtattt tatcgttttt cgcttgcttg aaaatattgt cgagacttct aaaggaactt
     2701 agagtcacta gaaaaataag gagtagactt gatctatcaa caattccctt cggggagtga
     2761 cggggtaggt tttaacgatt ggtaacgtaa aaaattggtc aatcgagtaa atgcgcttgt
     2821 ttggatagtg gtgaaatgaa tttgtgtaaa taataagcgc agagtaaaca agagggaatt
     2881 tagaagttag acaagttaga atagcgtcta gattaatcat gagcagctac taagggatct
     2941 agactttgac gcaaactcat ggaataagga gtaggtcttc tcgtaatggg gaggacgcac
     3001 aagaaattgt gtcgtgtgct gcagtacatg tagatacttg cgaggacgtt gattattgga
     3061 tcaatcgagc atttaccgct gtctactagg gtttctcgtt tcggacgcca taaacgagtc
     3121 gaaatttttt atgtattttt aagtaaacga aaaacgataa aatatgaaca agtcctgaat
     3181 aaagtgaaaa aattaatata aagcatagag aaaatgaaat ttacgtaatg gaggaatgac
     3241 aaatgaaaaa ttatgatccg aatatacgat tgggcacaca tactattaag gtttctttcc
     3301 aacgttggga ttacaaagga tttgtgacgt ttagaagggg cggaaattgt aaaggattag
     3361 atgttttagc attagacgag gatgatttat acgatcaaac attgactgat aatccaattg
     3421 gattcggctt gctacctgaa gatgatgaag gtaatgaatg gtttaaaatg acgcttatga
     3481 atgataatgg agacgaatta tctgttgaag atacttggtc atatttgagt gattatattg
     3541 tcagctttga aatcattgaa tttgtagcgg ataaagaaga ataaaaactg gaggaataaa
     3601 ttttacagat aaaggcttag aaggagtcac gacgattaat aaaaaggtag tattacctta
     3661 ggcattgagt aaggaatt
//
LOCUS       DAZULO010000162         3663 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000162 DAZULO010000000
VERSION     DAZULO010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.167"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..276
                     /locus_tag="ACG8OA_002798"
     CDS             <1..276
                     /locus_tag="ACG8OA_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFS4977527.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="ACG8OA_002799"
     CDS             419..640
                     /locus_tag="ACG8OA_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4977528.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="ACG8OA_002800"
     CDS             656..1498
                     /locus_tag="ACG8OA_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977529.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="ACG8OA_002801"
     CDS             1561..3363
                     /locus_tag="ACG8OA_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFS4977530.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3422..>3663)
                     /locus_tag="ACG8OA_002802"
     CDS             complement(3422..>3663)
                     /locus_tag="ACG8OA_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFS4977531.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1306 a          519 c          746 g         1092 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcccatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actactggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaataga tacccgtggt gctagttaat
     3421 tttaatatcc taaagaatat ctcaaagttc cttcaaaacg tcgttgtgct cgacttaata
     3481 gaacactgta agccagtaat atgctttcaa atctactctc tagcccttta acagaacgtg
     3541 agttgaaatt ttgacaacct aatttttcta aggaagaaaa aacagtccca actgtttttc
     3601 tttgttttcc aagtaagctg tggtctagtt tttctgattt atccatgttt ttacgtggcg
     3661 gaa
//
LOCUS       DAZULO010000163         3527 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000163 DAZULO010000000
VERSION     DAZULO010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.168"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(252..956)
                     /locus_tag="ACG8OA_002803"
     CDS             complement(252..956)
                     /locus_tag="ACG8OA_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977532.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(1143..1388)
                     /locus_tag="ACG8OA_002804"
     CDS             complement(1143..1388)
                     /locus_tag="ACG8OA_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977533.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(1405..2256)
                     /locus_tag="ACG8OA_002805"
     CDS             complement(1405..2256)
                     /locus_tag="ACG8OA_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4977534.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            2422..3426
                     /locus_tag="ACG8OA_002806"
     CDS             2422..3426
                     /locus_tag="ACG8OA_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFS4977535.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1097 a          600 c          687 g         1143 t
ORIGIN      
        1 gaaaatatgt ttattattag aattttttat agttaaggtt aggcatctta agaaatcagg
       61 caactccaag atatcttcag gtagaagaga agtcgcctaa cattgtagga tcataggtga
      121 tttgttggac aggtcaagct gatatttgag cctgatgtta gaaggaattt ctttttactt
      181 gctgtagaaa ttattatatg actaaaaagg ctgggatttt aaatcccagc cttttatgac
      241 aaatagaatt tttatcgatc agtaatcgct gtcgctgcaa attgttcgat caaaggaatc
      301 aaagtcaatt tgactttgta tttccgttcg agtgcttttt cgataacgaa ctcattttca
      361 gtagtcatag aaatagttgc taatttacga ggttcatcag catttttttc attaataact
      421 acttcatgtt cattttgttc tgtgtaaaat ttaggatact cagcaacggc ttttgaaaaa
      481 tcagcaaaac ttaaagcggc attcgtatca gttgagcgtg tattttcagt tgtgtccgct
      541 tttggttttt ccgttttttc ttctggcaca ctgactgttt cttctgtaac tggtgtcgca
      601 acagcttgcg cagttggttc atcgtttttc aatcgttgat cattccagta agacacgatt
      661 tcaataagaa gattgtcacg gaatcgttcg aaaatcccgt tttctcctga acgaattctc
      721 tttaatacag tattgaagtt gtcatcgatt ggcacgttga tccgtgtatc ttcatttgtt
      781 ttcccagcta atccgatcgt taagatttga cggtttttgg gatcaagcat gacttgtaac
      841 ggcaaaccat aacgtcgcaa gctctcttct tctagagaac ggaataattg tcctcttaca
      901 ccatccaatg aaggacggaa ttgatttaat aatttttgtt tctgtttttt cttcatatgt
      961 tctctccttc gctcataccc tttcgattat aagaaaaaaa cagccaaatg aaaaggagta
     1021 atgcaaaaaa acttgcgtat acgcaaatta caacagaaaa atttgcgtat tttcccgttt
     1081 tttgaaagaa aaataaaaaa tggaacggat tatgtatata tacataatcc gttccattct
     1141 ttctattttt tcggtatcaa ggcaaaaaca ggtagttcag gtgaataaat gacttcatgt
     1201 tcgtgccagg gtttgaccat tagcgaatga tcgaaccgtt ttttcagttc ttcgatgatt
     1261 tgatcatgtg tccagtttct tgcaggaaaa tgttggattt tctctgcatc gatcaaaaag
     1321 acaaaatcgg aatgaaaagt ctgggtaact tcttccgaga gagaattcaa gcgttcgatt
     1381 ggatccataa aatcaccttt cgacttaaaa ttgggtagta tctgggtctt ttgctttgcc
     1441 gactacacca tctaactgat cgatttgttt catttcttct tctgttaatt caaaatcaaa
     1501 taattcagta ttttctttga ttctgcttgg tgtcactgac ttaggaagag gaaggaattc
     1561 gtgttgcagc gaccaacgca aagcaacttg tgcaatcgtc ttgtcatgtg cttcagcgat
     1621 ttgtttcatt tcaggcacat cgaagatttt accagtgcct aatggactat atgcttcgag
     1681 gatcatatca tgtttttttg catactcaac tacagttgtc tgtaattcgc ctggtgcaag
     1741 gaaaacttga ttgaccatcg gcataatttt tgctgttttt gctagtgtat ctagatggtg
     1801 gggtaagaaa ttgctcacac cgattgcttt gattttacct gcttcataca gttcttcaaa
     1861 tgctcgccat gtatctgcat ttgcttgttc ccaattatca cgaaaagcaa cagggtttgg
     1921 ccagtggatc aaaaacagat ctaaataatc tgtttggagc ttcttcaatg attcttcaaa
     1981 agaagacatc acaagttcat agctgtggtt tgcattccat aattttgttg tcaagaatat
     2041 ctcttctcta ggaattccac tttccttaat cgcttggccg acgctttctt cattcttgta
     2101 tccttgggca gtatcgatat ggcgataacc tgcagccagc gcttctttaa cagaggaaac
     2161 agcgacatcc ccatcaggtg tttgccatgt gccgaatcct actaccggga tttcgtaacc
     2221 atttgataga cgataagtgc ttgttaatga attcatactt tcagctcctt ttttattcgt
     2281 tcaactataa gtgtagcgca tttttcgaaa aaaagaatcc attttgtttc tactcttgaa
     2341 atttttgttt aaaagaaaat gattttgatt ctggtatttt tgattttttt tccgtaaact
     2401 aagacagaag ggaatgagaa aatggatatc tatttaaaaa aagcaattct gcatattatt
     2461 gacagagaaa ctggttctcc agtcttttca caaaaagagc tggatctgac aaaagaatat
     2521 attcgtgatt ttcttcaaaa gaagatccaa aaaatctctt ctgctcaaac aaaaactgga
     2581 caactagcag aagaaagcat gatcgttgaa tcacttaaaa tcgctcaaga aaattttgtg
     2641 gaagcaagtg aagcaatcgt tcagcggtgg ttcgatatct accaagaaag tgaagaagca
     2701 cctagtgcag atgtatttgt tgtcttgtat gaattagata cagtgatgta cttatcttta
     2761 ttgaaagtca attatcgtga ggcttacact cactttgttg aagcagacga agcagggatc
     2821 gacaaccgct tgattttgaa tcgagcaatt ctaggggcga aaacacaaaa agcagatgaa
     2881 gcattcgctg tgaatttgac tgattggacc tacgaattaa ttgaaaaaaa atatgagttt
     2941 tctggcaaaa aagagcttta tttttctgga agagtgatcg aaagtgttcc tgcaccttct
     3001 ttagaagaaa atgtaaaagt gatccaaaaa gtagccaagc atcttggaaa aaaatttgag
     3061 acagaagaat ttgatatcat ggctgatttg aaagaagctg tctatgatac gatagaagaa
     3121 aaaggtcggt tggatcatga gatgattgcg gaaaaagtct tcaaggaaaa catcacggca
     3181 aaaatggctt ttaaagaaga agtacaagag caaggatttg tcccagaagc acctcctgta
     3241 aaagaagtac aagaaatttc tgagaaaaaa tttggaaaac aaaaattcaa gctatcaaac
     3301 gggatcgaac tgatcgtacc agtagatgta tatcgcaacc cagatttgat tgaatttgtg
     3361 aataatccgg atggtacgat atccgtgatg atcaaaaatg tggatgaagt attgaatcgt
     3421 ttataaaaat gactagaata ttaggtgttc aagtaccagt gatcagttaa atgaattgtg
     3481 tcaagagaag gatcggaaaa gacgaatata acaaaaaaag agcgtga
//
LOCUS       DAZULO010000164         3481 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000164 DAZULO010000000
VERSION     DAZULO010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.169"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACG8OA_002807"
     CDS             complement(<1..2232)
                     /locus_tag="ACG8OA_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4977536.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACG8OA_002808"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACG8OA_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          979 a          770 c          548 g         1184 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg aatccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacact tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 a
//
LOCUS       DAZULO010000165         3406 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000165 DAZULO010000000
VERSION     DAZULO010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.170"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..772
                     /locus_tag="ACG8OA_002809"
     CDS             <1..772
                     /locus_tag="ACG8OA_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HFS4977537.1"
                     /translation="YWGYSLPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDE
                     EKKVSYDKQYIEDLYYKYMETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIK
                     EFVTKEEVVIISVNFEYSHIDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLR
                     TSYKKLLSEREVVRDNAGLMAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIIT
                     KFALLDAMNVQMRDELSELAKKVKVTFLTDPRWVRIDK"
     gene            901..1485
                     /locus_tag="ACG8OA_002810"
                     /pseudo
     CDS             901..1485
                     /locus_tag="ACG8OA_002810"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            1431..1949
                     /locus_tag="ACG8OA_002811"
     CDS             1431..1949
                     /locus_tag="ACG8OA_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977538.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            1972..2997
                     /locus_tag="ACG8OA_002812"
     CDS             1972..2997
                     /locus_tag="ACG8OA_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFS4977539.1"
                     /translation="MKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVAVSLAI
                     FGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQRPSKVI
                     YDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAKKRGIT
                     ISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINSGELNK
                     DGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYNIQIVD
                     RVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYNLMNSG
                     GNGRVLR"
     gene            3246..>3406
                     /locus_tag="ACG8OA_002813"
     CDS             3246..>3406
                     /locus_tag="ACG8OA_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4977540.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         1229 a          381 c          674 g         1122 t
ORIGIN      
        1 ttattgggga tattcattac caaactatat ttcagcagtt cataatgctc atcctaatta
       61 tgcaagctat ctgtcatata agaatacttt aacaattgaa gcaatgaatg aaatattcga
      121 aatgatggat gaagaaaaaa aggtatctta tgataaacaa tatatagaag atttatacta
      181 caaatatatg gaaactggga aaatacagga ggaatatatg gaagaactga gaaaatttct
      241 ctcagacaaa aaaattctac tgattgcacc aggaaaaagt tctgtagatg aggtagaaag
      301 aataaaagag tttgttacca aagaagaagt cgtaataatt agtgtaaatt ttgaatattc
      361 tcatatagat atagattacg tatttttaag taatttaaga cgttttcatg aattaccgaa
      421 agaaagcaga actaaatgta ttgttacatc aaatatacaa gcatcggatg tatatttacg
      481 gacgagttat aaaaagttgt tatctgaaag ggaagttgta agagacaatg caggattaat
      541 ggcaattaga tttttagccg atatgaatgt ggatgaaata ttcttagctg gatttgatgg
      601 atattcacat aatgaagatg agaattatgg tgagcaatcc atggaaatta taacaaaatt
      661 tgcattatta gatgcaatga acgtgcaaat gagagatgaa ttgtcagaat tagctaaaaa
      721 agtaaaggtg acatttttaa cggatccacg ttgggttcga attgataagt agtagtaata
      781 atgtttttaa aattttgata aagagatagt gagagaataa aaatgggtaa agtcttaaat
      841 aaaaaagaag aaaaatattt aagggacgat ggggcgagat gaaactctaa ttttgaactt
      901 ttaagaatca tttctatatt gtgtattgtt gcttaccatg ctaatttgcg tttaaattat
      961 aatgcagacg gaattatagt acatggttca ttaactgcta accagatttt cttgaattat
     1021 attggtattt ggggtcagct aggagttaat tgttttatat ttatttcggc atatttttta
     1081 agtgagaaaa gaaacattca ctttgaaaat gttttgggaa ttgtattaga atgtattttt
     1141 tatagtgtat tagtttatgg aattctttgt gcatgtggaa tagaaacatt ttcattgaac
     1201 agctttgtaa aagaaatatt atcatttgct ctaggtagat attggtttat tacatcctat
     1261 ttagcattgt atattctaag tccgtattta aatatcatgc ttaacaatct ggaaaaactt
     1321 cagttgcaaa aaataagtat gttattgttt ataggtgtat gctgttatcg tatgttattt
     1381 aaaacatctt tgtttgggga ttgtgctgtt ttaattgcac tatatgtcat atgttataca
     1441 ctgaaaaaaa cgataataat atttttgaga gacacagaat cataggaaca attgtatgta
     1501 taacagttat accacttctc atatccatag gaatactttt caaatttata ccaaatgcaa
     1561 tggttaacca gatggttcat attggttctc cattgatggt tatagcaagt atatttttat
     1621 tttatgtatt taaaaatttt tcattttaca gtaagtatat aaataaactt gcgagaacca
     1681 gtttaggggt atatatttta catgaaaatc attatttcat tgatggtatg aagtctagag
     1741 gcttaatatg ggatgggata tttggattag gcaaaaggtt tttagatagg gatttttgga
     1801 tttggtttat aattgcagtt gtagttgtgt gcataatttg catggctatc gatgttgtaa
     1861 gagaaaaatt tgaaagacag atttttaaaa ttaagagtgt aaagaacctg tgtaaactta
     1921 tgaatcatgt tataaatata tacccataat taataaatga ggagaaaaga agtgaaagtt
     1981 atatcattcg gagaattatt attgagactt gcagcacccg gttattcaaa gttatttcaa
     2041 agagatagtt tggagactac attctgcgga ggggaagcta atgtagcggt atccttagca
     2101 atatttggat atcaatcaga atttataaca aagcttccgg attcagatgt tggatatgcg
     2161 gcagctaatg ctttgcgtta ttttggagta aatgttgata atattatatt tggagaggga
     2221 cgcatgggac tgtattattt agaaaaagga gcagctcaga gaccatctaa agtaatttat
     2281 gacagaaaat tttctgcaat ttcgttagct gatagtgatg aatttaagtg gaaggagatc
     2341 ctaaacgatg caaattggtt tcattggaca gggataaatc cagcattgtc tgagaatctc
     2401 tttgagacgt ccttagatgc atgtcaggct gccaaaaaaa gaggtataac aatttcttgt
     2461 gatttaaatt atcgatccaa gctttggaca tcagaggaag caaaagaaaa gatggaacag
     2521 atactaccat tggttgatat ttgtatttgt aatgaagaag atgcagaaaa agtgctagga
     2581 attaaagctg agcatacaaa tattaatagt ggagaattaa ataaagatgg ctatgtgcag
     2641 gtggctaggg agattcacaa gaagtttgga tgtaggtatg tagctacaac tctgcgtaga
     2701 agttattcgg caagtagaaa tggttggagt gcaatgttat acgaagcaga aaaagataaa
     2761 gcgtattttt ccacagaata caacatacaa attgttgatc gcgttggtgg tggagacagc
     2821 ttttctgctg gtataatata tgcgttatgt gaaaatatgt ccggacaaaa ggctattgaa
     2881 tttgcgactg catctagttg tttaaaacat actatagaag gtgattttaa tcgaactaca
     2941 attgaggatg tatataattt aatgaatagc ggtggaaatg gtcgcgtgtt gcgttagtca
     3001 acatataaag ataaatcaca gtaattttta tggtgattat ctgtcttagt agtgattaga
     3061 ataacgtgaa aataaattgt tgagcgttta tactgttttg aaaaataaat ttaacatatt
     3121 gaaaaataca aatatatacg tatattataa agcgccgatt gtaaaattaa gctagacaac
     3181 taaaacagtc atttgtgcta cactcaaaat agttccgacc aaagaattat aaggagtgag
     3241 tgcaaataac ctatacctat cttacaacgg atgaacttgt aataatagag tcttatttga
     3301 aaattaacca atctgttgct aaaactgcgc attgcttgaa tcgttcaagt caaacgatcc
     3361 ataaagtata tctgttcttc aagcaaagaa aatcagcctt agaata
//
LOCUS       DAZULO010000166         3237 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000166 DAZULO010000000
VERSION     DAZULO010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.172"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            103..1347
                     /locus_tag="ACG8OA_002814"
                     /pseudo
     CDS             103..1347
                     /locus_tag="ACG8OA_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="ACG8OA_002815"
     CDS             1360..1992
                     /locus_tag="ACG8OA_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4977541.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2200..2424
                     /locus_tag="ACG8OA_002816"
     CDS             2200..2424
                     /locus_tag="ACG8OA_002816"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFS4977542.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2526..2759
                     /gene="relB"
                     /locus_tag="ACG8OA_002817"
     CDS             2526..2759
                     /gene="relB"
                     /locus_tag="ACG8OA_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFS4977543.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="ACG8OA_002818"
     CDS             2749..3018
                     /locus_tag="ACG8OA_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4977544.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1135 a          478 c          638 g          986 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gcttttttat ggctctttgt caaataggac tgatagagtg agataagaac atctaaggta
     3181 gaattccttt tctactttag atgttttttg attcataggt aatcagatta tttagta
//
LOCUS       DAZULO010000167         3221 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000167 DAZULO010000000
VERSION     DAZULO010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.173"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACG8OA_002819"
     CDS             301..585
                     /locus_tag="ACG8OA_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977545.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACG8OA_002820"
     CDS             588..1004
                     /locus_tag="ACG8OA_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977546.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACG8OA_002821"
     CDS             complement(1141..1860)
                     /locus_tag="ACG8OA_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4977547.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2544..2630
                     /locus_tag="ACG8OA_002822"
     CDS             2544..2630
                     /locus_tag="ACG8OA_002822"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS4977548.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..>3221)
                     /locus_tag="ACG8OA_002823"
     CDS             complement(2932..>3221)
                     /locus_tag="ACG8OA_002823"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977549.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DAZULO010000168         3220 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000168 DAZULO010000000
VERSION     DAZULO010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.174"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(440..1054)
                     /locus_tag="ACG8OA_002824"
     CDS             complement(440..1054)
                     /locus_tag="ACG8OA_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4977550.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1660..2037)
                     /locus_tag="ACG8OA_002825"
     CDS             complement(1660..2037)
                     /locus_tag="ACG8OA_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977551.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2337..3218)
                     /gene="galU"
                     /locus_tag="ACG8OA_002826"
     CDS             complement(2337..3218)
                     /gene="galU"
                     /locus_tag="ACG8OA_002826"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS4977552.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          978 a          659 c          498 g         1085 t
ORIGIN      
        1 ttttgtgtat tttatttcta ttagaaaaag gtatcctgtt ggacaatttt gttatatcag
       61 gtaaacttca ttgcaataat gataacaaat gtactataat tactttgtaa agagttttgg
      121 ggaagactct ttcatcaacg tgtattaaga gacaagaaag aatccatatg gattctttct
      181 tgtctctttt tccactactt tttatttttc tgacactaca tattcttata actcatttct
      241 cataactgtg aaacaactta taaaaataac aaactttaaa caaaacaagc ttaaaatcaa
      301 agatgatcca tcttaaaata actaaaacaa aaaaagtagt cgttgttaat cctcattaat
      361 aatcgttttc tttttttcaa acgctccatt tttatgtgga acattatttt tttaaggaac
      421 tttgtcaaca tgtggataac tattttccac ttgtgtcctg gactagctgt tttgctgtaa
      481 aatacaaaac ttgctgcagc aaaaagatcg ttgcaagaga ggctgttccg cagatcgctg
      541 gatctagatt tggcaaagtc aagactgcat catgtaaatt ggcgtggttg tccaaaacca
      601 aatcagcaaa ttcaaataat cgcaaaccac tgctatgttg cgatttgata cttcgtgaca
      661 aatcaaatcc agtaacaatg attaatggat gaccattttc tttgatccac tgagctagtt
      721 cgatgattgc cgggtttctt ccttcattcg aaagaagaag aaaaatgtct tctgatttga
      781 cagaaagctg atcaataatt gcctgtcctg caccttctag cgtttcatag acacctccgg
      841 ctggattttc aatcaatata cttggtacaa gatccggggt ttgatcccaa aattcaaatg
      901 ctacaccact tagtgtgcgg ctagcatata tctgcaatct accaccttga ttaatttgtt
      961 tcgcgatcca agtgccagct tgaagcatca gcggctcttc tatccttaat ttctcttcca
     1021 attggtgata atagtcaaaa taacataaac tcatttatct ctcccgccta gctatacttg
     1081 acatgaatag aaacagctgc ctaatctaag gaagtacata aaaaaacagt agttagacag
     1141 taacacatgt ggtctttgct gtttcaaaca caaaaaaggc attgaacgaa tacgcccaat
     1201 accttgacat agcaaataaa acaacgtgca gaccatggaa aatatctaca taggggtaga
     1261 attttcaccc tagattaggt acaataaatt aggtcgatgc actgcattga ctatggcaaa
     1321 atgatcggtc agggtgtatg tgtgtgactg gtcattccct gattcttagc gccaactaag
     1381 ggtcagggag ccttttttta ttcagctgtt tctatctgaa tttattgtaa gtaatctctt
     1441 atccaagtgc aagcaagaaa atacaaaaaa acattggtat gatttatata tatataaata
     1501 acataaaaaa aggaatggct tatcccgata caacaagcat tcctttaaaa aagattcttt
     1561 tatataagtg aaaaaagctt ttttccacca attaaataaa tctctcatct caatagttta
     1621 tccattattt tatctttttt tgcgtatacc tactaaatat taccgtcttc ttctcgttct
     1681 tctactttgt tcgagagaat ctgtactaga aaaagtagtt gtatgtctac cttttctgcc
     1741 tatccgagac tttcttttca tatatgggat ataaatgatt agatacagta cagcaaaaaa
     1801 taaagcaaat acgcaagctt cccaactacc taacgccaat tcaaccatca aggtagcaac
     1861 cccaacggat gctgcatacc agataaaaat cagaaacgtt tctttttttc tgaccatgcg
     1921 aaacatgata aatagactaa taagcaatag tggcaacaca aatatccaat tgcttccttc
     1981 catcagccac ttgagtatat caagcagcga ctgtgcaaca gcgactaatt ttcccatagt
     2041 gaacacacac ctttttttat aattaaaaaa ttcgcctttt tattctacta tgaatcttct
     2101 ggcttggctt tcatttctct tatgtaatca ttttctttta tacaacttaa aaaaaaatta
     2161 aaccattagc ataatatttt ctgaatctat tcacgaaagt ctttttctat ctttcgtttt
     2221 ccacgactca actgatctag agtcaaaaaa ctaaaacgac agttgcgcaa atgacgcaca
     2281 agctgccgct ttaataacaa atagcctttc attctcagca ggccacttat ttccagttaa
     2341 ctttctgttc ttagcttttt actcaactgg ataatatatg cacttaatga agtcccaatt
     2401 tccgaatgtt gaagtccata ttcaatattt gtcttcatat atcctaactt atcgcctacg
     2461 tcgtatcttt ttcctttaaa ctctctcgca tagaccgcct ctgtctggtt aagagtctga
     2521 agtgcatccg ttagctgtat ctcgtttcct gctcctggtt cttgagtttc taatatctca
     2581 aatattttag gagtcagtag ataacgtccg ataatggcta aattacttgg tgcttttccc
     2641 atttcaggtt tttctacaaa cccttttact ctgcaacgac ccttttcgct atctgtttta
     2701 ggatcaataa ttccgtattt agatgtttca ttttctggta cacgcatgac ggcaacagcg
     2761 gaagcttgtg tttcttcata atccatcatc aattgttgag tcaatgggat atcatctttc
     2821 atcaaatcat cccccagcat gacgacaaac ggttcatttc ctataaatgc ttttgcttga
     2881 agcacggcat cccccagtcc tcttggatga gattgacgga tgaagtgaag attgacttga
     2941 gtcgtttctt caactaaagc gagtaattct gttttttcct tttcccgcaa attgttttcc
     3001 aattcgatat ttgcatcaaa gtgatcttca atgggtcttt tttcttttcc cgttacgatc
     3061 aagatgtctt cgattcccga atccaatgct tcttccacaa taaactggat cgttggttta
     3121 tccacgattg gaagcatttc ttttgccatg gcttttgttg ccggcaaaaa acgtgttcct
     3181 agcccagcgg caggaatgac tgcttttttg accttcactt
//
LOCUS       DAZULO010000169         3044 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000169 DAZULO010000000
VERSION     DAZULO010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.175"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1849
                     /locus_tag="ACG8OA_002827"
     CDS             <1..1849
                     /locus_tag="ACG8OA_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFS4977553.1"
                     /translation="IESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPIT
                     ADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWK
                     EKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTV
                     DLTDNVTNLPTLPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVT
                     DNRSDADKYEPTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGN
                     YEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDASEGIKNKE
                     DLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEG
                     QKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEE
                     VEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNF
                     TVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQ
                     DKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1983..>3044
                     /locus_tag="ACG8OA_002828"
     CDS             1983..>3044
                     /locus_tag="ACG8OA_002828"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFS4977554.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESI"
BASE COUNT         1202 a          428 c          622 g          792 t
ORIGIN      
        1 tattgaaagt ggaacaacag tctttgcagt gaaagacggc gtaagcttac cagaagataa
       61 acttccagtg ttgaaagcaa aagatggtta tacagatgcg aaatggccag aggaagcaac
      121 tcaaccaatt acagcagatg atacagaatt tgtatcaagt gcaacaaaac tggatgataa
      181 atctgacgct gacaaatata atcctgaagg tcaaaaagtg actacagaat tgaataagga
      241 accagacgca tttgagggaa ttaagaacaa agaagattta ccaaaagata ctaagtatac
      301 ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa aaagggactg ttgtagtgac
      361 atatccagat ggatcatctg atgaagttga agtagatgtt acagtgacag acaatcgttc
      421 tgacgccgat aaatatgagc caacagtaga aggcgaaaaa gtagaagttg gaggcacagt
      481 agatctaaca gataatgtaa ccaacctacc aacattacca gaaggaacaa cagtaacaga
      541 tgtcactcct ggtggtacga ttgataccaa cacaccaggt aattacgaag gggttatcga
      601 agtaacgtat ccagatggta cgaaagatac agtgaaagtt ccagtagaag tgacagacaa
      661 tcgttccgat gccgataaat atgaaccaac agtagaaggc gaaaaagtag aagttggagg
      721 cacagtagat ttaacagata atgttactaa cttgccagcg ttaccagaag gaacaacagt
      781 aacagatgtt actcccggcg gtacaattga taccaacaca ccaggcaatt atgaaggtgt
      841 cattgaagta acgtatccag acggtacgaa agatacggtg aaagttccag tagaagtgac
      901 agacaatcgt tctaatgcag ataaatacaa tcctgaagga caaaaagtga ctacagactt
      961 aaataaagag ccagatgcat ctgagggaat taagaacaaa gaagatttac caaaaggaac
     1021 tacttatgct tggaaagaga aagtagatgt tagcacagct ggaaataaaa aaggtactat
     1081 tgtagtgaca tatccagatg gatcatctga tgaagttgaa gtagacgtta cagtgacaga
     1141 tactcgttcc gatgcagata aatataatcc tgaaggacaa aaagtgacta cagacttaaa
     1201 taaagagcca gatgcatctg agggaattaa gaacaaagaa gatctaccaa aaggaactac
     1261 ttatacttgg aaagaaaaag tagatgttag tacagctgga aacaaaaaag gtactgtagt
     1321 agtaacatat ccagatggat ctaaagaaga agtagaagtt actatttctg tagtagataa
     1381 aaaagcaccg aataaacctc aagttgatcc tattacagag ggtgaccaaa ttgttactgg
     1441 taaaactgaa ccaaatgcag aggtgacagt tacattacca gacggaagtc aacaccatgg
     1501 gacagctgat aaaaatggta attttacagt taaagttcct aaattagagg cagggacaaa
     1561 agtgatagta actgcaactg atgaatctgg taatactagt gaacctacta atgtagttgt
     1621 ttcttccaac aaaaaagaca gtggaaaaaa cggtagtaaa ggtagtaaga ctgacaatca
     1681 aggtagcaac agcaatcaag acaaaaatag aggcaaaagc cagtcctcaa aagtttttcc
     1741 taagacggga gagagtgaca gtaatatctt tactattagc ggtggtttaa tactgttagg
     1801 aacgttagga ttattagggt ataaaaatcg aaaaaaagag aatgaataga aaatcaattt
     1861 cttaagtctt ctctgagaag aggaagaatc tttttcttcc tctttttgct tttttatgat
     1921 gggaaataag tgttagttaa agatgaatat actttttata gaaaggaaaa gaatgtaatt
     1981 aagtggaaaa aatagtaatt attggttcaa accattcagg gctagccgca gctcgatttc
     2041 taaacttaag cgattcaaat tatgaagtta ttctaatcga taaaaaatca gaaattagtt
     2101 acttggaatg tgggacatcg ttatttataa aaggagaagt aagtaattta gaagatcttt
     2161 tttatcaaaa tagaatgaca ctaaattctg agataaaaaa gttatttcta gagacagaag
     2221 ttgacaaaat tgatattcaa aataaaaaga ttagttgtgt aaaaaaagat ggtacaagat
     2281 tctctcaaga atatgacaaa gttatattag ctacaggttc aaagcaaatt atgctagata
     2341 taccggggaa taatttagct aatatttttt cgataaagaa ttttccaaat gcaaaagaga
     2401 tttttaaagc tttggatgat agtactatta aaaaagtagg aattattgga gcaggatata
     2461 ttggagttga aattgctgaa gccataagaa aaagagggaa agaagtatat cttttcgatg
     2521 ttgcagatag agtgttgtcg acttattacg accgtagttt ttcagataaa gttgaagaaa
     2581 tactatcaaa aaatggaata catctttgtc tatcagaaag tcctaaaaaa tatttaggaa
     2641 gaaaaaagat tgaaaaaata gtcactacaa gtggtaccca atactctatg gatatggtcg
     2701 tacaagctat aggatttcta tctaatagtc caattgggga aaaggaactc ttgagagatg
     2761 ttagtggagc atatctaact aatgaatacc agcaaacaaa tataaaagat atatatgcta
     2821 ttggggattg tgctacaact ttttttacgt ccattaatag aagtagtgtt gatttttcta
     2881 tttctaatgc tctaagaaca gcttatattg caactcaaca tattaacaat caagatttta
     2941 caccttctgg ttcccaattt acaaatggct tttctatatt taattacaat ttttacgcag
     3001 caggtttgaa tttaaaaaca gcacgattat taagtgagtc tata
//
LOCUS       DAZULO010000170         2788 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000170 DAZULO010000000
VERSION     DAZULO010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.176"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..1064
                     /locus_tag="ACG8OA_002829"
     CDS             57..1064
                     /locus_tag="ACG8OA_002829"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFS4977555.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1076..2080
                     /gene="neuB"
                     /locus_tag="ACG8OA_002830"
     CDS             1076..2080
                     /gene="neuB"
                     /locus_tag="ACG8OA_002830"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFS4977556.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2077..>2788
                     /gene="neuC"
                     /locus_tag="ACG8OA_002831"
     CDS             2077..>2788
                     /gene="neuC"
                     /locus_tag="ACG8OA_002831"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFS4977557.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPII"
BASE COUNT         1037 a          357 c          541 g          853 t
ORIGIN      
        1 ttttaaagat taagaatcga attatagatg actataattc ataggaggag atataagtgt
       61 ttaaaggaaa aacaatatta gtaactggcg gaactggttc cattggaagt gaaattgttc
      121 gtcagctttt aaaatatgaa ccaaaagcta tcagaattta tagtagaggt gaagaaaaac
      181 aattttatat gcaacaagag ctctcaaatc acactaattt gagattttta attggtgata
      241 tcagagactc tcaaaggatt gattatgcat gtcgtgatgt ggatattatt tttcatgctg
      301 ccgcaatgaa acacgttcca gcaagtgaat ataatcctat ggaagctgta aaaacaaatg
      361 tgatgggaac tcaaaatgta atagatgcag cgatcaaaaa taatgtaaaa cattttgtag
      421 gtatcagtac agataaagta gtgagtccta ctaacaccat gggtgcgaca aagttattga
      481 gtgaaaaatt aataatagct gccaatcagt ataaaggaaa aacagaaaca atattttctt
      541 gtgtaaggtt tggaaatgtc atggggtcaa gtggatctgt tattcctctt tttagtaaac
      601 aaataaaaaa caataaagat attactttga cagatgttga tatgacacga tttatgatgt
      661 caataccgga tgctgcaaga ttagttctgt ctgctgctat gaattcaaaa tttggagaaa
      721 catatgtctt aaaaatgccg gcaatcaata ttttaacttt agctcaatca ttaataaaaa
      781 tatataatga tacacatgat gaaaaatata gaggtgaaat aaaagttgta ggtatcaggc
      841 caggagaaaa attatatgaa gagctaatga ctacagacga aattgaacgc tcatatgaaa
      901 atgataaaat gtatatcatt ccaccacact atcatgctac tgacgaactg tacaaagatt
      961 atgaaaaaat taaaatcact gcatactctt cagatgcaac agaattaatg agtggacagg
     1021 aatttattag aatcgcaaaa gagaataatg tcgtgacttg gtaaggagtt ttatgatgaa
     1081 atcagaagta tatgtaattg cagaaattgg tgtaaaccat aatggaagtc tagatttagc
     1141 tttagaaagt attgataaag ctaaagaatg tggagctaac gcggttaaat ttcaaacatt
     1201 taaaacgaat aagctagttt ccaaaaaggc tccaatggca aaatatcaga aggataatct
     1261 taaagaggac agttctcaat ttgaaatgct aaaaaaatta gaattatctc aatctgattt
     1321 tattagaata aaagattatt gtatacaaaa taaaattgat tttttgtcta ctccttttga
     1381 tgaagaaagt gcagactttc taaaacaaat tggtgtacaa ggatttaaaa ttggctcagg
     1441 agatttaaca aatttacctt ttcttagaaa aatagatatg tatgggttgc cagttttatt
     1501 atctacaggt atggctgata ttcatgaggt cagtgaggct gtagattgtt tcatagatag
     1561 tccagttact attttacatt gtacttcaaa ttatccggct agttctgaag atattaattt
     1621 attggcgatt aacacattaa aagaaatgtt cgggataccc gttgggtact ccgatcattc
     1681 attgggatat gatgttggta tttgtgctgt tgcgatgggt gctaaggtaa ttgagaaaca
     1741 ttttaccttg gataaggaac tacctggtcc agatcataaa gcttctttag atcctcagga
     1801 atttgcagat tttgtaaagc atattagaaa cactgaaata tttttaggtg atggtgttaa
     1861 gcgagcaatg ccttctgaac ttagtacaaa agaagtatca agaaaaagcc tagtggtcac
     1921 tgagaattta aatgtgggag atattcttac agagaaatct cttacaataa aacgtcctgg
     1981 aaatggaata gctcctaagt ttttatatca atatattggc aaaaaagtta aaagaccaat
     2041 aagtgcagat agtgttctgc ttgaggaaga tgttgaatga aaaaaatact tatagtaagt
     2101 ggaacacgag ctgattatgg aatatataaa cctgttgctg agaatttact acaagataat
     2161 tgggatgtat catttgttgt ttctggaatg catttatccc ataaatatgg atttactaaa
     2221 gaattgatct tagctgacgg atttagaatt agtggagaag ttcaaagttt gtttttagag
     2281 aacaataagg gaaacatggc tcggtctgtg gcattagaga taaatggttt cacaaatata
     2341 tttgaacaaa ctagccctga ttttgtatta ttactagggg atagaggaga aatgttggca
     2401 gctgcgatag cttgtttgta tcttggaatc aaaacagcac atattcacgg aggcgaagtg
     2461 tctggtactg tagatgaatc aataagacat gccataacga aaatagcatc tgttcatttt
     2521 gctgctacgg aaaaaagcaa agagcgttta ataaaaatgg gggaagattc ttggagagtt
     2581 tttcccgtcg gagctcctag aatagatact attttaaata atagattacc tagttttaga
     2641 gatataaaag aaaaatatca tctaaaattt gaaaagaaaa aatacgtttt gcaagtattt
     2701 catccagttg taaccgaact agaaaatata gaaaaacaaa ttttagcttt gatcaattca
     2761 atggaacaac aatctcaacc aattattt
//
LOCUS       DAZULO010000171         2712 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000171 DAZULO010000000
VERSION     DAZULO010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.177"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..519
                     /locus_tag="ACG8OA_002832"
     CDS             175..519
                     /locus_tag="ACG8OA_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFS4977558.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            520..939
                     /locus_tag="ACG8OA_002833"
     CDS             520..939
                     /locus_tag="ACG8OA_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFS4977559.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1031..1381)
                     /locus_tag="ACG8OA_002834"
     CDS             complement(1031..1381)
                     /locus_tag="ACG8OA_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977560.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1347..2677)
                     /locus_tag="ACG8OA_002835"
                     /pseudo
     CDS             complement(1347..2677)
                     /locus_tag="ACG8OA_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
BASE COUNT         1005 a          491 c          523 g          693 t
ORIGIN      
        1 aaaataaaaa aagcgttagc aactatgctt aaaaagcata tcgactgaat aaactaagca
       61 ttggacaggc tgaaagacta catttaaaat gaaatgcgaa gaaacaatag aaaataaagt
      121 tgccttagaa ccatagttga atcacctaac agtcgtgaag ggagaataag agaaatggca
      181 gaaaaacaaa ccgcaggacg agatcaatta ggagattttg caccaaaatt tgcagaattg
      241 aatgatgatg ttttatttgg acaagtatgg tccagagaag aattatctgc tcatcagcgt
      301 agtatcatca ccatatctgc attaatttca ggaggaaatt ttgaacaatt gccagctcat
      361 ctgaagattg gcaagcaaaa tggcattact agtgaagaaa tagcagaaat cattacccat
      421 ttagcttttt atgtaggatg gccaaaagct tggtcagctt ttagtttagc aaaagaaatc
      481 tatttagaag aaaatagcga agataagaaa gaggcgtaaa tgacgacaaa acacgaagaa
      541 atacacaatg gcgtgatttt tccagcaggt gaaaaaaacg aagcttacgc tcaagtattt
      601 accgggcaaa gctatctgaa tatgttagtg aatgatcccg aagtttctgt tggtgtaggt
      661 aatgtaattt ttgaaccagg ttgtcgcaat cactggcatg tccataaaga cggctatcaa
      721 atcttacttg tgaccggagg agaaggctgg tatcaggaag aaggaaagcc agcaagacga
      781 ttaatggctg gagatgtcgt cgtaacgcac aaagggatta aacattggca tggtgcgaca
      841 aaagacagtt ggtttgaaca tttggcgatc actgctggta attcagagtt tttagaacct
      901 gtaacagatg aagtgtatga aggattggaa aatcaataat gtcaagcttg aacagctcct
      961 gaaagtagat tttaatcgaa ctttcaggag ctgctttttt gattcagtct tttataaata
     1021 gaaaataggg ttaataatag gaatgagaag cacgtcgaaa gatcttaata ttgataatcg
     1081 gctgggcata catgatttcc acgtcatcaa tcaaactacc taacagatga taatcttccc
     1141 gatcatgtcg gggattacct aatagttctt gatagtagat ttccatacta gggtcccgag
     1201 gattgttcag caaatcagca agtacatcta aatcttctgg ctgagtttct gccgtaccct
     1261 gtatctctat gaaaagccct tcctcagcaa attctactcc aatacgtatt tcagttattt
     1321 catgggaata taaataagta agaagctcat cgatgattct ttttgataat tctttttcca
     1381 agtgtctttt tttcctttct gacagcggta aagttatcta gtatcggtac aaggatacct
     1441 gcaacgaaac cagttgaaaa accactgtta tatagattta gtcctccgtg caaagtgctg
     1501 acgttatgga caagagcaat atgtagtaca ccagctatta caccgtaaaa actgccgtaa
     1561 aaaccgctta tgggcgcgag acctgtccca aacacagctg caacaagcat gccagtactg
     1621 gtgatctcat gaaaagttcc aaacaaagaa gcaataaata ttccgactag tatcggaaaa
     1681 ctgtttttta aatgacaacc aaaagcactg aaacccaccg cagataaaat agcaccgatg
     1741 acaggtccat ttaactgacc gccaaccagc aaaacataag aagacagcat cagaccaact
     1801 aagcctatgt tgatcaaagt agcaccaata ccagaattag caatgaaatc agttgtcaat
     1861 tttccagatg aatcgaaaat ttctcgaatc cccaaaagcc gaaaggaatt ataatagaaa
     1921 ccaattccga ctgtcagcga aaaaaagcca aaaataatcc aaatgagagg aaagtgatat
     1981 tcattgaaaa ccaatgtatt ttcgacgatt gaatagctga ataacctcaa aacagcagta
     2041 aaagtcatcg caatcaaccc agaggtgaat ccaatattat aaagtgtgaa tccacgatgg
     2101 aaaactaaag tttgagcagc taagggcgga agaagtagac caattgctat gccagctaaa
     2161 ttccccaata aaatacctat cagtaaagga aaacgcatac caaaagtgat ataactaacg
     2221 accggtgata atgcgctacc aaacagtccg atcatgatgt actgagaaaa aggtttattg
     2281 acaaatacag cgtatagata cacacctaaa atgattgaga tagaattata aaggtttttg
     2341 ccaaaaaaag agaaaccggt aactgttaga atcgaagcaa ataagggacc gtttaaaatc
     2401 accttgtgcc gataagctaa aagcacagaa agtaaggtca aaatccctac atttatgaat
     2461 gcgcttccga aaccgcctaa agcaaaataa tctgtcaaaa gattgcttgg cgaggtaaga
     2521 atagttagca atccactcca attatcagta ggagtattag aaagcaagcc aataaaaaga
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc
     2701 tttaactata ac
//
LOCUS       DAZULO010000172         2693 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000172 DAZULO010000000
VERSION     DAZULO010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.178"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            367..>2693
                     /gene="esp"
                     /locus_tag="ACG8OA_002836"
     CDS             367..>2693
                     /gene="esp"
                     /locus_tag="ACG8OA_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFS4977561.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          974 a          416 c          533 g          770 t
ORIGIN      
        1 cttatgtcac agtctcattc agtaatgcgg gataaggctc cttattccgt tatttgaata
       61 ctctgaagtt taatctaaga aagggtaata ttttgaaaac atgcttcatc gcatagaaat
      121 gaacttaatt tcagaaagaa tacatacata tctttttata tgttttcagc tgctgaatgg
      181 ttaaaaagca caaatatccg aaaaaaacat gaagaaatgt actttttttg ttgtaatgtt
      241 catgaaattc tctaagtaaa ttagttaaca taaggttagt aagattccta ttctgtgaat
      301 aatacgtatc agatttatct tttattctta ctaggaaagt aattattaag ggagttgatt
      361 tgaataatgg ttagcaagaa taataagaga gtatttctag aaaaaacaaa gaaacgagta
      421 ttgaagtaca gtatcaaaaa attaagtgtt ggggttgcat ctgttttgat tggtgttggt
      481 cttgtcttgg gaacaactga actagttaaa gcacaagatg aaatttctcc aagcagccca
      541 cttgaaacag cgataagctc cgtacaagta ggtgacaaag tagcttcggg gaatactttt
      601 caggagaatc ctggttatac gaaaaattat aacttttcgg atttacaatt tagtcctcaa
      661 gaactaactg gagacacact aaaaggcaat acaattggat ttgaagttta cggaaaacat
      721 aatattgcag catcaactaa aaactgggaa atccgtcttc aattagatga acgattagct
      781 aaatatgtag aaaaaattca ggtcgatcca aagaaaggaa ttggatcttc tagacggacc
      841 tttgtaagaa ttaatgattc gctgggtaga cctacaaata tttggaaggt caattatatt
      901 cgtgcaagtg atggattatt tgctggggca gaaacaaccg atacgcaaac cgctcctaat
      961 ggggtaatta cgtttgaaaa gagcctagat gaaattttta aagagatagg catagacaat
     1021 ctcaagaccg atcgtttaat gtatcgaatc tacttggtaa gtcatcaaga cgatgataaa
     1081 attgtaccgg gtattgatag tacgggctac tttttaacag attcagatga tttctataat
     1141 agcttagatg tatctgaaaa taatcctgac cagtttaaac atggatcagt aagtgcaaaa
     1201 tatgagaaac ctaatactca aacaaaagat ggttcaggat ccacaggtgc taatggagct
     1261 ataatcttag atcataagtt aacgaaaaat tataatttct catattcatc ttccgcaaag
     1321 ggcacgcctt ggtatgctaa ttacaaaatt gatgaacgat tagttccata tgtagcgggt
     1381 atacagatgc acatggttca ggctgacaaa gtaacatatg atgtttcgtt tgaatccgga
     1441 acaaaagtag ctgatttagc aatcgagcga cgggaaggtc atgaaaatta tggcataggt
     1501 tcaataacta ataatgattt aactaaactt atcgattttg ctaatgcaag tccacgtcca
     1561 gtcgttattc gatatgtttt acaattgact aagccattag atgaaatctt agaagatatg
     1621 aaagcaacag cacaagttga agaaaataaa ccatttggtg aagatttcat ctttgattct
     1681 tggttgtcgg atacgaataa aaaattaatc cagaacactt atggaacagg ttattattat
     1741 ttgcaagata ttgatggtga tggaaaccct gacgataaag aagagagcgg agacacgaat
     1801 ccatatatcg ggaaacctga attagaagaa gtatatgatg ttgacacaac agttaagggg
     1861 aaagtattca tccacgagtt agcgggaaca ggtcacaaag cccaacttgt tgataaagaa
     1921 ggtactgtat tagcagaaaa aactatcgct ccaaatgaaa aagatggggc tccaatttca
     1981 gatactgtag aatttgaatt tacgggtgta gattcaagta aattaatcgc gaaagatgaa
     2041 ttaaaaatcc aaatcgtttc tccaggtttt gataaaccag aagaaggttc aaccgttatt
     2101 aaggaatcac caaaagcggt tgataaacaa accgtggtag ttggatttaa accagatgct
     2161 aaagaatcaa ttcggaataa taaaaactta cctgaagatg cagagtattc atggaaaaca
     2221 gagcctgata cttctaacgt tactgatagt acgaaaggta ttgtaactgt taagatcgga
     2281 aatcgaactt tcgacgtgga tgtagagttt gctgtaaaag cttctcaagc tatggaaaat
     2341 gatgcaacat acgtacctat aacaacaact ccagaaacga cagttcaaag tggaaaacct
     2401 acatttgata aaccagatgt tcctttagct aaagatgcct tttcaatttc agatgtctat
     2461 aataagtact tcggtaatgc aagtgttgat gcaaacactg gtattgttac attcactcca
     2521 gctaaaggtg taggagaatc ggagccgatt actggaacaa ttcctattaa aattgtttac
     2581 caagatggtt ctgtaggcac gaccgattta gcagtaactg taagtaaaga tatttatgaa
     2641 aatccaggag aaaacattcc tgcaggctac cacaaagtaa ccttcaccgc agg
//
LOCUS       DAZULO010000173         2586 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000173 DAZULO010000000
VERSION     DAZULO010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.179"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..2543
                     /locus_tag="ACG8OA_002837"
     CDS             102..2543
                     /locus_tag="ACG8OA_002837"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFS4977562.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT          868 a          457 c          611 g          650 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttat
//
LOCUS       DAZULO010000174         2510 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000174 DAZULO010000000
VERSION     DAZULO010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.180"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            206..2359
                     /locus_tag="ACG8OA_002838"
     CDS             206..2359
                     /locus_tag="ACG8OA_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFS4977563.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          866 a          430 c          549 g          665 t
ORIGIN      
        1 aagaaaacat aattttcaaa aataagttta ttttttaatg aaaaaaatag ttggatatgt
       61 aactttaagt gattgctgtg aaacaaaaag ttgattttat cttgaaatta ggtaaagaat
      121 ttcttgctcc gcccattttt tcttttttat taagaagttt ttgggtacaa tgaagcatga
      181 atgtaaatag gcggaaggat aggaaatgat gaaagatttt atgaaaaaaa tattaaatac
      241 ttcttggtta gaaaaaataa gaaaaggtgc acaagaacct aagaacaaaa cgtttcgccg
      301 gtcccatgtt ccttttcgct taaattttct ttttttcatt atatttacgt tatttgttgc
      361 gttgattgct agattgggtt atttgcagat cgtgaacggc gaagagatgg aggcgcgagt
      421 gaagtctact tctacgatta cggtccaaga aagtactcct cgcggaatga tctacgactc
      481 cacaggaaaa gctttagtta cgaataaagc gaatcaggcc atcacatata cgagaggaac
      541 acaagttacg gctaaagatt tgcttgaaat cgcaacgaag ctgaactcgc tgatcgatgt
      601 tccggtagat gaaaatctga cagatagaga taaaaaagat ttttggctgg ctgatcccaa
      661 acatttgaaa gaagccacaa aaagactttc ggcaaaagaa aagcaattag ggaacagcga
      721 acaatatagt gcgatggtca ataaagtcac agctgatgag attaatttaa acgaggagca
      781 attaaaaatt gctaccatct ttaaacggat gaatagtgca tatgaattaa acactgtcta
      841 tatcaaaaac tctggtgtta ctgatcaaga gttagctgtt gtggcagaac gcgcttctga
      901 acttccagga gtttcgactg gcacagactg gacacgagaa tataatgcag caagttcact
      961 gaaaagtatt cttggcagtg tgacgactga aaagcaagga cttccagcag atgaagcaga
     1021 gaaataccta gcaaaaggtt attctcggaa tgaccgtgta ggacaaagtt atttggaaaa
     1081 acaatatgaa gatgtcctgc aaggaaccaa aacccaatat gaagtttccc ttgataatga
     1141 aggaaatgtt tcgaatcaaa aagaaatctt ctctggggaa aaaggttcta atttaatgtt
     1201 atctatgaat gcagaattcc aatcaaaagt agaagaaatt ttgaaaagga attatcaaac
     1261 attgatcaac aacggcaaag cacagtattc gcctggtgct tatgcagtgg ctatgaatcc
     1321 tcagacagga gaagtgctag caatgactgg tttcagtcat gaacaaggtt ccaaagaaat
     1381 caccgagaat gctttaggca cgattactag cgcctttgat ccagggtctg tagtcaaggc
     1441 tggtactttg acagcaggct gggcaagtaa cgcgatcagc ggaaatcaag ttttgattga
     1501 tgagccgatt cgtctgcaag gtgcttcaga aaaaagctct gtctttaatc gaagcggaca
     1561 agttgctctt gatgcagtca aagcatcgga attgtcttct aatacgtata tgatcaaagt
     1621 ggcgttaaag atgttaggac tagactatac acctggaatg ggactaccta gtttagatga
     1681 agaagctaaa gcttatcagc aattgagaga ctctttcaaa gagtttggtc ttggtacaac
     1741 gactgggatc gatttgccga atgaatctcc tgggatctca cgttctgttg attatatgaa
     1801 gaagttcaat gcggataatg gcaaagaatg gtatgctcca ggtaacttta ccgatttggc
     1861 atttggacag tttgacacat atacaccaat ccaattagct caatatgctt caacagtcgc
     1921 taatggcggg aaacgagtac agccgcacct agtcaaagcg atctatggca atgacgaaaa
     1981 tggaaacttg ggtgaagtga agaaagaaat tggcacaaca gtagaaaata cggtcaatat
     2041 ttctgcagaa aatatggcga tcctccgtga aggattccac caagttgtcc atggtaccga
     2101 tccttacaca acagccaaac cattagccag tgcgaaaatg gacctttccg caaaaacagg
     2161 gactgcagaa acggttgcgg aaggtcatcc agatattgcg acagtcaata gtaatatcgt
     2221 ggcatacgga ccaacagaca atccggaaat tgccatcagt gttgtcttgc cgaacttatt
     2281 agacgaacaa gaccacataa acttgacgat tgctaaagaa ataatggacg cgtattatga
     2341 tatgttcatg gcaaaataaa aaagaggtcg tgaaaaagcc gcttagtttc gagtatgaag
     2401 aaaaattttg tgaaaatagc ttctcacatt tttgcttaaa tttgacttca taccgagaaa
     2461 ctggcttttg aacaccgttt actcggcaaa aaaggttgtg acaaaaattg
//
LOCUS       DAZULO010000175         2473 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000175 DAZULO010000000
VERSION     DAZULO010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.181"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..624
                     /locus_tag="ACG8OA_002839"
     CDS             85..624
                     /locus_tag="ACG8OA_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977564.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            1644..>2473
                     /locus_tag="ACG8OA_002840"
     CDS             1644..>2473
                     /locus_tag="ACG8OA_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS4977565.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          905 a          326 c          397 g          845 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt tta
//
LOCUS       DAZULO010000176         2436 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000176 DAZULO010000000
VERSION     DAZULO010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.182"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..272)
                     /locus_tag="ACG8OA_002841"
     CDS             complement(<1..272)
                     /locus_tag="ACG8OA_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977566.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
     gene            complement(309..446)
                     /locus_tag="ACG8OA_002842"
     CDS             complement(309..446)
                     /locus_tag="ACG8OA_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFS4977567.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(448..894)
                     /locus_tag="ACG8OA_002843"
     CDS             complement(448..894)
                     /locus_tag="ACG8OA_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977568.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(891..1040)
                     /locus_tag="ACG8OA_002844"
     CDS             complement(891..1040)
                     /locus_tag="ACG8OA_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977569.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            complement(1054..>2436)
                     /locus_tag="ACG8OA_002845"
     CDS             complement(1054..>2436)
                     /locus_tag="ACG8OA_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977570.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGSTATPYQPNLL
                     DDPYWVGKTSLGENIANKGTTFPIKSSAYEIYKGNTEEELIIGQTYTITLKGTKPASQ
                     TFVAYNYWNVNFGELKPVEGLTDVWSLTFTPTKLEPGLPKDLRIFQLPKETAGACQID
                     WLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDITPEYTEKGLNDTVSLTEPQSI
                     EGLKNFEDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNG
                     KSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGS
                     AYSFVITYSI"
BASE COUNT          638 a          502 c          420 g          876 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcatagtg gaacgaatag tcgaacttga tcgtcagtaa aacagcccca atcatacatc
      421 attttcacat cgctaaaact aaacatacta ctcacctcct tttgaagctg gatttagttg
      481 ctctttaatt tctgaaatgt ctttgctatt ttgtaacgaa gcaagcatca tttttgaatt
      541 gatttgtgct aaactgtcag ctttttcttt caatgcagta ttttcctgtt taatcgctac
      601 atcgcttagc atgagtttgg catttagctg ttttaggttg ccgttttcat gttcaagagc
      661 ctcgtacatc gctttgagat tgtttaaatc gttgtgatct aacgcgttcg ctaaaataat
      721 ccattggttc aatttaggat caaacatctg atcagcaatc gttagcggtt cgccatctga
      781 acgaacccct tcgagcggtg gctgatctgt gtaaggaacg gatacaagca tgtcgtccaa
      841 tacttttcct gcatactctc cgccagtacg gccatatttc caaatgtttt tcatttattt
      901 ccctcccagt aattgaattt cggtttccaa tttggaatcg gtggttcgac ttctgtgcat
      961 tcttccggta aatgttcttc gtcattcaca atgatttgct cgaatccgta aggcttgatt
     1021 gatttgtaaa ctactttcat tagatttcct cccttaaatt gaataagtga taacaaatga
     1081 ataagctgat ccataacttg agtttcttcg ccatttaatg gctccatctg caccaataga
     1141 taactgagca ctgttcaaag tagaacggtc tatcgagcca accagttgct caaaactaat
     1201 tggtggccga tagccttctg gaattgttag tatcgttgaa tcatttccac cactgctttt
     1261 cccattcaac gcaaaaaaat aaatagtgac ggtttttcct tcacgataaa gctttgctga
     1321 tccggtattc ccgtttgtaa ctgttaaggt tacgacttca tatttgttgt cgtctgatgt
     1381 caatacagat tttccttttg attgaatacc atcttcgaaa tttttcaatc cttcgatgga
     1441 ttgcggttca gtcaaactaa ccgtatcatt caagcctttt tcagtatatt caggcgtgat
     1501 gtcccagctg tagtcgttcg gattgttgct atctttcaat ccttcaccaa agtatttaaa
     1561 ctgactaata ttcggggttc gggtgtcgcc tttttcaatt ttgagccagt caatttggca
     1621 tgcgccggcc gtttctttag gcaactgaaa aatgcgaagg tctttaggca aacccggttc
     1681 aagtttcgtt ggtgtgaatg ttagagacca tacgtctgtc aatccctcaa ctggttttag
     1741 ttctccaaaa ttaacgttcc aataattata tgctacaaag gtttgacttg cgggttttgt
     1801 tcctttaagc gtgatagtat acgtttgacc tattataagc tcttcttccg tgttaccttt
     1861 atatatttcg tacgcgctag atttaattgg gaacgtcgta cctttatttg caatattctc
     1921 acccaacgat gttttaccta cccagtaagg gtcatctagt aagttaggct ggtacggtgt
     1981 ggctgttgag ccttcttcga ttttgatgtc gtacaactta aatccgccat ttattttatc
     2041 cctgtcaaca aagctaatac tcatataaaa tcgatctaaa tttgtgattt gataattaac
     2101 gttagctgta cctttgattg ttatttcttt ccctacatca tctgttgtaa tatttgtact
     2161 atttgcttcc aataatatct tttctcctgg tgatgtacga tacaccaaac gtaatttatc
     2221 aatagctcct gtagttcctt catcaaatcg aactttcgca ctcagagtat aggttttccc
     2281 actagcaagc tgaggtgtat taatacgcgt aaacataata atgcttcctg taccatcaga
     2341 agtaaaatgt agcttttcgt tatccgaagt tagtgacccg tggtaaccaa cgttaaaatc
     2401 gctcgatttt agtttggaca ttaaattcgg cttacc
//
LOCUS       DAZULO010000177         2407 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000177 DAZULO010000000
VERSION     DAZULO010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.183"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1150
                     /locus_tag="ACG8OA_002846"
     CDS             5..1150
                     /locus_tag="ACG8OA_002846"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFS4977571.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="ACG8OA_002847"
     CDS             1321..1779
                     /locus_tag="ACG8OA_002847"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFS4977572.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="ACG8OA_002848"
     CDS             1938..>2407
                     /locus_tag="ACG8OA_002848"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFS4977573.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DAZULO010000178         2400 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000178 DAZULO010000000
VERSION     DAZULO010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.184"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACG8OA_002849"
     CDS             207..2069
                     /locus_tag="ACG8OA_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4977574.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="ACG8OA_002850"
     CDS             2069..>2400
                     /locus_tag="ACG8OA_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4977575.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAZULO010000179         2334 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000179 DAZULO010000000
VERSION     DAZULO010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.185"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..461)
                     /locus_tag="ACG8OA_002851"
     CDS             complement(<1..461)
                     /locus_tag="ACG8OA_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4977576.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
     gene            complement(745..1032)
                     /locus_tag="ACG8OA_002852"
     CDS             complement(745..1032)
                     /locus_tag="ACG8OA_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4977577.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(1022..1351)
                     /locus_tag="ACG8OA_002853"
     CDS             complement(1022..1351)
                     /locus_tag="ACG8OA_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4977578.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(1428..1700)
                     /locus_tag="ACG8OA_002854"
     CDS             complement(1428..1700)
                     /locus_tag="ACG8OA_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS4977579.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(1700..1963)
                     /locus_tag="ACG8OA_002855"
     CDS             complement(1700..1963)
                     /locus_tag="ACG8OA_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4977580.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            2197..>2334
                     /locus_tag="ACG8OA_002856"
     CDS             2197..>2334
                     /locus_tag="ACG8OA_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFS4977581.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
BASE COUNT          745 a          446 c          304 g          839 t
ORIGIN      
        1 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
       61 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
      121 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
      181 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
      241 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
      301 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
      361 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
      421 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
      481 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaga
      541 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
      601 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
      661 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa agaagccgtc
      721 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
      781 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
      841 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
      901 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
      961 tttcaatggc ttctctttta gcttttttaa atacttaatg acatgttttg aaggaacgat
     1021 ttcataagcc aatttctgtt gccaactcct ctttagtcat tactttagaa ttagtaagtg
     1081 tatcttcagc aatatctcta aaggaagaat gcattttgtt ttttctattc ttaaattcta
     1141 caagaatctc atctttatcg tagccttctg caattatatc agacaaaata tcttcgctaa
     1201 aatcataaaa ttctttttgt ggttcaacaa attcataaag aataccattc tctaccaact
     1261 ttgcttcaac ttcgactcca ttaggaacat taaaatcttt aggtactgtc aacatgattg
     1321 aattgccttg ttttcttgtt tttgtaatca tttaaatccc tcatttctaa cagtatgtaa
     1381 ttacttagta aatacattat accacgagat aatgtattta caagtaatta ctgtgactcg
     1441 taatgcgtcc ttgctgaata aatttcaacc acgtgttcat cttcatctac cttataaaca
     1501 acccggtgtt gtctattcaa tcttctagaa tacctacctt catgtgtagg ttttaacttc
     1561 tcaaatgatt gggtagggag atagggatct tcctttaaag tctgaataac cttctcaaat
     1621 tgaggtttca aattagactg tttgattttc tttaaatcag ctttagccga gtttttaatc
     1681 atcacagaat agttattcat tataagttat cccaatcaat ttcatcaaca ttagtaaatc
     1741 cactatcatc tttttctcgc ttacgaacaa cacccaaagt gcctatttgc tcaagtaata
     1801 gcgtttcttt gattgctttc caatcatcca aaccaataag caccgcatta ttttcactgc
     1861 gctcactaat gatttcaatt tccgtatgat tctcatttac atctttcaac aacttataaa
     1921 aatttttccg tgctgagcta ggatttaaga ctgtttttgc catatctact ccccctctaa
     1981 tttatatctc cattctaacg tacttaatta agtacgtaaa ttcgattgtt ttgtttaaat
     2041 tggagtacca aaacagcatt gtaaagtgtt atccgtttcc tttatacccg tggtgctagt
     2101 taattttttc ctagaggaaa atgtctaaac atgttggtaa atcaacaaaa aaacgcatag
     2161 agtagtctca aaactcccaa gaaatgagga ctacatatgc gttcatcaaa atatactgaa
     2221 cattatacag atacttttat aacattcaag gaaattatga gaactgtttc taacatatac
     2281 cacaattgtg taccagataa gattaaaaac cgaagaaata ctgatcaact gaag
//
LOCUS       DAZULO010000180         2231 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000180 DAZULO010000000
VERSION     DAZULO010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.186"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..>2231
                     /gene="acm"
                     /locus_tag="ACG8OA_002857"
     CDS             116..>2231
                     /gene="acm"
                     /locus_tag="ACG8OA_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFS4977582.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLL"
BASE COUNT          875 a          345 c          441 g          570 t
ORIGIN      
        1 ctatcaattt tttagttaaa agattggaaa tgagtggggg aggaattgaa gaaaggattt
       61 ttgagagatg atatagtagg ttttattttt agttaaaata ggggagatag aaaaaatgaa
      121 aaagtgtaga aatattattt tatcaatgtt atttattatt actaatataa catcactgat
      181 tcctgttcat gtctatgcgg atgcaggcag agatatcagc agtaatgtca cttcgttgac
      241 cgtggaccca acgaatatta cagatggagg aaacataaaa gtaaaattct cgtttgacga
      301 aaaaaaacaa aatatccaac caggtgatta tctttggatc aattggccaa gtgaaggaaa
      361 tattcgcggt gaaggattcc aaaaagaaat cccattaatg attgaaaata aaaatgttgg
      421 aactttaacg gtcagaaaag attctgcgca ggttgtgttt aatgaaaata ttaaaaacct
      481 agactcagta gaaggttggg ggcaatttga gatacaggcc agaaacgtaa ccgataccgg
      541 tgaagaaaat acgggaccat tcaccgtgac gagcggtgat aaaacagcta ctgtaaatgt
      601 gacaaagcca gcttctggtt cttcttctag cgttttttat tataaaacag gagacatgct
      661 acctgaagat actaagcaca ttcgatggtt tttgaatatt aacaataacg gcacttatgt
      721 tgaacaacca gttaaaatca gtgatgaaat ccaaagtgga caaagattag atccaagtac
      781 gtttgaaatt aatcaaatac acttaggtga acagaaggtt tatagaggag aagagggaat
      841 ccaacaattt ttacaagatt tccccagcgc tacatttaat ttttcagtaa ctgataatta
      901 cattgaaata actataccaa aaaactttgt caatcttagg aaaataatgg tgagttataa
      961 gacgatcata gaaaacccag aacaaataaa ttttgagaat cattcggaag cttggtttaa
     1021 agaatttaat aaacctgctg ttgatggaga aagtttcaat catacagtta aaaatatttc
     1081 agcttctggt ggagtcaacg gtacagttag aggcgaattg aagattttca aatatatcaa
     1141 tgacacagag attggtatcc caaatgtgac tttcgaatta agacgagcag atgaacagcc
     1201 tattcaagga cagtctagta tcctgttgac ttcgaatgaa cagggagaag ctagtatcaa
     1261 aggcctgcaa gtaggagact atgttgtaaa agaaaaagaa gcacccaatt ggattgattt
     1321 tgatccacta agttcaaatg aactcaaatt ctccataaac gaaaatgata cagagggagt
     1381 cagtttacct atttataaca agaaaaaggt tacgaacatc acagcaacta aaatttggaa
     1441 cggtggaacc actccaagac cgagtattta ttttaaatta ttcagagcaa gtacaaacaa
     1501 ttgggaacca gttcctgatg cagaaacaaa acgattagac aatggaataa cttcagtgac
     1561 ttgggaagat atccaacagt atgatgatag tggaaacgaa tatacgttta aagtgcaaga
     1621 agtagatcaa aacggaaatg attatgtccc ttcaggttat caaaaaattg aaaacgggct
     1681 agtcgttaca aatgagaata aagaagtcat ttcagtctca ggacaaaaaa catgggaaga
     1741 caatgagaac caagacggca aacgaccgac gacgatcaca gtaaatctat tagccgacgg
     1801 tcaaccgatc caacacaaag aagtaagtga aaaagatgat tggcgctatc gtttcacaaa
     1861 tctgccaaaa tacagagacg ggcaagaaat catctatact gtaacggaag acaacgtgcc
     1921 cgaatacagt acgacgattg acgggtataa catcaaaaac agttatacac cggaaaaaac
     1981 aaatatatct ataccaggta gccatataac accgtggaaa aattttccag agaagacaga
     2041 gagagagaaa attagaaatc tattctctca aaaattgcaa atatttgctc atacaggaga
     2101 atccagcgaa caaagaagca attatattag taagagagct gtacctaaac agataacaaa
     2161 taaatcaaaa agcattcttc ctaaaacaag tagtaaaaaa tctagttttg ctgtaatcat
     2221 tggtttattg a
//
LOCUS       DAZULO010000181         2122 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000181 DAZULO010000000
VERSION     DAZULO010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.187"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACG8OA_002858"
     CDS             complement(<1..914)
                     /locus_tag="ACG8OA_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFS4977583.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACG8OA_002859"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACG8OA_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFS4977584.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          717 a          374 c          412 g          619 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aa
//
LOCUS       DAZULO010000182         2065 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000182 DAZULO010000000
VERSION     DAZULO010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.188"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1008
                     /locus_tag="ACG8OA_002860"
     CDS             <1..1008
                     /locus_tag="ACG8OA_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977585.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="ACG8OA_002861"
     CDS             1025..1174
                     /locus_tag="ACG8OA_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977586.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="ACG8OA_002862"
     CDS             1171..1617
                     /locus_tag="ACG8OA_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977587.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="ACG8OA_002863"
     CDS             1619..1756
                     /locus_tag="ACG8OA_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFS4977588.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..>2065
                     /locus_tag="ACG8OA_002864"
     CDS             1794..>2065
                     /locus_tag="ACG8OA_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977589.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          753 a          358 c          425 g          529 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa acttt
//
LOCUS       DAZULO010000183         2058 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000183 DAZULO010000000
VERSION     DAZULO010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.189"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..337)
                     /locus_tag="ACG8OA_002865"
     CDS             complement(<1..337)
                     /locus_tag="ACG8OA_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFS4977590.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
     gene            complement(404..559)
                     /locus_tag="ACG8OA_002866"
     CDS             complement(404..559)
                     /locus_tag="ACG8OA_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFS4977591.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1176..1397
                     /locus_tag="ACG8OA_002867"
     CDS             1176..1397
                     /locus_tag="ACG8OA_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977592.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1398..1655
                     /locus_tag="ACG8OA_002868"
     CDS             1398..1655
                     /locus_tag="ACG8OA_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977593.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1791..>2058)
                     /locus_tag="ACG8OA_002869"
     CDS             complement(1791..>2058)
                     /locus_tag="ACG8OA_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005727 - extrachromosomal circular DNA
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rep family protein"
                     /protein_id="HFS4977594.1"
                     /translation="SDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDL
                     IDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          678 a          462 c          314 g          604 t
ORIGIN      
        1 ttttagcata ttgaaatttt tctggattat ccatatgagc aaaatatcgc accatacctt
       61 ttgcattagc acatttttgc gggtttggag accgaagctt ttgagttatc tcacgtattt
      121 gatcatacgt ctttttaccg ctaaacatta tcaaaacatg ccaatgaggc tttttcaatt
      181 caccgtcagg attcacatct ttatcatgaa gtggactctc aacccaagga acatgtaact
      241 catctaaaaa ctccctccaa ttttcaggag ccgattcagg atagactaca aacgtccaat
      301 tccttgtgcg ttcatcttta gtttttctac tcgtcatgct ataattacct taattgaaca
      361 acagtctaac agcaaaagca gtgcgccaac actaatgctt ttgctatttt ttttgctcat
      421 tttcctttcc gttagcatta acccaaattg taataagacc agacttacta agacccattt
      481 gtttcgcaat tttatcaaga ttatctgcca cttccttagt aagcgttaca acgactctct
      541 ttttttcatt actgatcata attcgtatcc tccaacgact ttttcttaaa aaccatagta
      601 acaaaaaagt aactaaggtg caacagttta gatactaatt agcgaataat aggccttaaa
      661 cccttggcat tactgatttt gtcaaaaaaa aacgctgaaa cccttgggag agtaaggcta
      721 aacagctatc gaccaatgac caatgaccaa taaggggggt gtcgtagtcc ccccctcctc
      781 cgcccaattc ataaacacca aaaccaaaaa acaaacctca gggctccgcc ccgaaccccg
      841 acctccttct accgaaatat atgcaggcgc cacgcttact atttcgttca gctatcggct
      901 tttgcaatct aacacgaccg tttcgctacc agtcgcctta caggctccat tctcgcttct
      961 cttacgtgtt ttaacgattg cgccgataac taaacgaaat agacgctaaa tcgcttaaaa
     1021 cgcaaaatag acccttttta gagcattgat tttttctact agtcatttcg ataatataaa
     1081 attgaccaca acacagaaaa cttatattat aagcttatgt cgtaagtccg acgtactcac
     1141 gtaatataga cggacgttaa atggaaggag cataaatgag agtgataaaa tcagaaaaat
     1201 tcactttggc cgaaattatg acgaccagcg aacttgcaca agaacttgat tacaaccaag
     1261 cttacgtttt gcgcttagcg aaagaacatc tgactgaagg aaaagaatat cgctctgccg
     1321 gtagaagaaa ctatcttttc agcccagaag cccttaaaaa actagaaaaa gtcataacca
     1381 ccaaagaagg tgagtaaatg cctaaagaaa caactatcaa cgttgaacaa ctagaaaaat
     1441 tgctagaatc atattccaat gattttatcg aattgaccgg cggaaaagtc gaagaaacca
     1501 ccgacctctt tgacctactt ctcacaaata ttctggaatt aaactctgaa gaacaaaaag
     1561 agcataaggg aaaactgata gcaccttcct acaggaaggt ggctaagtcg gctgtgccaa
     1621 ctggttttct tacaaaacaa ccttatattt tttgatatca caaaaaaatc cattttggac
     1681 ctattttcgg tcataatata gtacctactt ttggtcatag tttcgtccgt agttttggtc
     1741 atagtgacta agactttcac gcacaaaaaa agagctgaaa ttaacagctc ttatccttta
     1801 tttatttttg aatttacact tccaccatga cgattcgatt ttatatattg cccgataata
     1861 taagctgaat tatcacaaag aagcggaaac caatcatcaa atctttctga cattgcatag
     1921 tcaattaaat ctttgatttc agtgatattc ttagaatcaa caaacgacat catctctcta
     1981 atcaactcat atcgttctgc actcgttact gacaaataac tagcaatttc tgcaccacca
     2041 tgagcgataa tatcactt
//
LOCUS       DAZULO010000184         2055 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000184 DAZULO010000000
VERSION     DAZULO010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.190"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..365
                     /locus_tag="ACG8OA_002870"
     CDS             <1..365
                     /locus_tag="ACG8OA_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4977595.1"
                     /translation="CVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNKIR
                     HSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIHSS
                     IHYLTPQEFEELAKEKMA"
     gene            complement(721..1293)
                     /locus_tag="ACG8OA_002871"
     CDS             complement(721..1293)
                     /locus_tag="ACG8OA_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4977596.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1309..1914)
                     /gene="fic"
                     /locus_tag="ACG8OA_002872"
     CDS             complement(1309..1914)
                     /gene="fic"
                     /locus_tag="ACG8OA_002872"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFS4977597.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          638 a          425 c          308 g          684 t
ORIGIN      
        1 attgtgtcat tcaaacattg aataaagcaa aaatacatta tcacattcca gaaggaatga
       61 ttctacacac tgacttgggc agccaataca cagcaagaga agtggaacaa tggcttaaaa
      121 ccaacaaaat aaggcattct tatagtagaa aaggaacacc ttatgataat gctggaatcg
      181 aatctttcca tgcctcattg aaaaaagaag aagtctacac gactagctac tcagattttg
      241 aagaagcaaa tcgagcgcta tttagctaca ttgaaggatt ttataaccga aatcgaatcc
      301 atagctcgat tcactatcta accccacagg aatttgaaga attagcaaaa gaaaaaatgg
      361 cttaaccgtc tctactaaag acaatcaaca ccgtagggat atggagataa aatcaataga
      421 gtcaatagat tatttatcga taataacact aaaaaagcca cgttcctaaa cgtgacttac
      481 gctgaacgat atgtgttgtt tggtagacga acaaatcgtt cattataaat atatgatggt
      541 atcctaatat agtaagcgtg gctagcatac ctttaattac tttacctttt acagttacca
      601 tacacatttt ttgagcagaa atcaagtaaa taaaaaaata ttatatttca caacatgcta
      661 atagactcat atctagaacg cattaagtat ttacctgtct ggttcgctga gacttctttt
      721 ttaactttct tcaattttct tctttattct aaaaagcgta ctgcgactga aaccggttat
      781 agcttcaact tccttatacg atttacctga agtaagcagt tcatatgcgt gcctcttctt
      841 cggtgtgatt gttgctttcg gacgtccttc tctgaaatta ggattattct ttcttgcaaa
      901 catttttcct tcttgggtac gttccacaat catatcacgt tccatttctg ctacactcaa
      961 caacgtccgt gtcaccattc gccccatgga cgtattttca atcgttccta gattcaaaac
     1021 cttgatcgtt acgttatcat cgagtaaagg ttcaatgatc tctaatgctt ctctcgtgtt
     1081 acgagcaaat cgatcaagct tcgtaacaac tatttcatct ccaggcttca gaccagccaa
     1141 cagctgattg aattttggcc gatcccgttt tgtacccgta aatttttctg aaaaaatatt
     1201 cttcttctct acgcagttct caagcaactg ttgaatttgt tcttttgtac tttgagaagc
     1261 ggttgagaca cgtgcatatc catatttcgt catttggtcc acctactttt acaaatcatc
     1321 tatcgtatac tcagaatacc cttcatagaa atagctgaca tcgattcctt tcatatataa
     1381 ctcgcgatcg tcaattttat cagtcagagc attggaaatt agatagcgta tttccaaatc
     1441 gtttatggga cttcgctcca ttgcagaaag atagtctgct ttatcgatta agttccaatc
     1501 tacaactttt tgcaattctt cctttaaaat gagatccagc caaattctag tacttcggcc
     1561 atttccttct cgaaatgggt gagcgatatt catctccaca tatttcttga taatctcttc
     1621 aaaagaagtt tgtggcatct gatcaatatg ctgtaacgaa tgttctaaat acataactgg
     1681 tgcaaatcga aaatttcctt ttgcaatatt gaccgatcgg atttttccgg caaaatcgta
     1741 aatatccgaa aatagaaatt catgaatttc agataaacct ttaaatgtac caacttcgat
     1801 ttcatttatt tttccacaat cgtacaattc tttcgctttt tgtttactga gtttttcttc
     1861 aaccttagcc aactcaactt gattggttaa tcccaattta ttctctaaaa tcatgcgaaa
     1921 tctccttaac tttatgacat aagtttatga aacactctca accccattat atcgcgattt
     1981 tgtttatgtg tcaaaaacta ttaagtttct gacccccgat agatttttca agtgtctcta
     2041 ttttgcacag tctag
//
LOCUS       DAZULO010000185         2003 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000185 DAZULO010000000
VERSION     DAZULO010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.191"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACG8OA_002873"
     CDS             <1..378
                     /locus_tag="ACG8OA_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFS4977598.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACG8OA_002874"
     CDS             426..1826
                     /locus_tag="ACG8OA_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFS4977599.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DAZULO010000186         1993 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000186 DAZULO010000000
VERSION     DAZULO010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.192"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ACG8OA_002875"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ACG8OA_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS4977600.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ACG8OA_002876"
     CDS             852..1040
                     /locus_tag="ACG8OA_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS4977601.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ACG8OA_002877"
     CDS             1053..1610
                     /locus_tag="ACG8OA_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4977602.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ACG8OA_002878"
     CDS             1642..1884
                     /locus_tag="ACG8OA_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4977603.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          308 c          406 g          555 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc ctt
//
LOCUS       DAZULO010000187         1992 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000187 DAZULO010000000
VERSION     DAZULO010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.193"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            69..1238
                     /locus_tag="ACG8OA_002879"
     CDS             69..1238
                     /locus_tag="ACG8OA_002879"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS4977604.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(1505..>1992)
                     /locus_tag="ACG8OA_002880"
     CDS             complement(1505..>1992)
                     /locus_tag="ACG8OA_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977605.1"
                     /translation="LNYSSVVNMVKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKD
                     QLLLKNHQVTEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNA
                     TVLKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSH
                     E"
BASE COUNT          705 a          338 c          302 g          647 t
ORIGIN      
        1 ttatcaagat acaagctatt ttagctttat tctttctgta aaaaagaaag gagactaaat
       61 aaactattat gaaaaaagga aaacatgcta aaaaaagaaa atattcattt ttaatgatcg
      121 ttgtcgtgtt gtttagcctt agtgcgttct ctacaggaac tattcttgga tggcaatttt
      181 acaataatga acactctcag atagttgccc atgctcaatc agctcaaaaa aagttgatac
      241 tgactaccaa tgaacaacag ctcgttgaaa aaatcgatca actattaaga aaaaatgatt
      301 atattggctc aatttacgta aagaaaaata atcgcatcat catggaaaaa ggctatggct
      361 atgcgaataa actcacgcaa caaccaaatg atccttctct ttattatcag attggttcgg
      421 ttcaaaaagc attgactact cttttgattt taaaacaagt cgagcgtggg cacctttctt
      481 tagatactaa gctggctcaa ttctatccac aaattgctgg cagccagcaa attactatcc
      541 gtgacttgct gtatatgcgc tcaggcttgc accgaactgc ttctccaacc ataccgatga
      601 ctgatgaaga agtcgttcaa tttgcgattc agcacttaaa atttacagat tatcagacct
      661 ctcattatga ttcattgaac tttaccatat tgactggaat tttgatcaaa ttaacaaatc
      721 aaccgtatga aaaattattg accgatgata tcatcgaacc tttaggatta cagcatacaa
      781 aattttatga ccaagtcaaa aattcatcca atcatgcact atcctatcaa atgtctaaag
      841 aggatgatta ctttaaacca ctgacagaat ctgaaacaga gatccataac gaattaggga
      901 ctggtaatat cagtatgtcc gtctatgatt taaatacgct gtttacacaa gttttatctg
      961 gaaaactagt ttccaaagac ttactcttct cattatggcg tgaaaatgcg aatggacatc
     1021 catatagtgg tggggtatat agcggagata attatatcct gtcgcaagga aatatcgact
     1081 cttttgatac atctgctata tttagcaaag attcacagga tgctatcatc atggaaagca
     1141 atataaaagc tgacaaaact atcaaactgc ctactgctga tctaagaaac caaatctatg
     1201 acctgatgga aggaactact aagttggcaa atagctgata tgatgaaaag atagcaaata
     1261 ctacttcttt gaatagcatt tgcttaaaaa aagtactcga ctaaacgtag gaaagagtgt
     1321 gggacaaatc taaaaatcaa tttttgctac agactttaat tccaaataaa cagaacagaa
     1381 agaggtgttc ttctttctgt tctgtccaaa ttattctaag atttctcttt accaacatta
     1441 tttgacaaag ggcctattaa atctaaaata gtatctttag attgctaaaa tatgtgctag
     1501 caaatcattc atggcttgtt cttattttta attttatgca ataaaaaaga agaacaatta
     1561 gcgtgattat agaaataaat aatgagattt tagaaaaaaa tgtgtatgaa tacctgattg
     1621 ttactttatt tattccactt tcacttttta ctgtaacaaa aggacctcct tgttttagta
     1681 ctgtagcatt ttttgaatta acagtaaccg ttaatccttt ataccggaat acaggtaaaa
     1741 ctattgtttt gccttttgaa gatttaaacg taaatgtaat ttcatcttta tttggagaaa
     1801 agctactata aataacttga ccatcttctg taacttgatg gtttttcaaa agcaattgat
     1861 cttttttata gcctataggt acataatcaa aattctcaaa ttcttcaagt ttattttcca
     1921 tttgttcatt agaataagtt ccttgaaaat tcatttcttt caccatgtta actactgatg
     1981 aataatttaa aa
//
LOCUS       DAZULO010000188         1889 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000188 DAZULO010000000
VERSION     DAZULO010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.194"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..609
                     /locus_tag="ACG8OA_002881"
                     /pseudo
     CDS             83..609
                     /locus_tag="ACG8OA_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            764..1339
                     /locus_tag="ACG8OA_002882"
     CDS             764..1339
                     /locus_tag="ACG8OA_002882"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFS4977606.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1382..1579)
                     /locus_tag="ACG8OA_002883"
     CDS             complement(1382..1579)
                     /locus_tag="ACG8OA_002883"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977607.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            1545..1748
                     /locus_tag="ACG8OA_002884"
                     /pseudo
     CDS             1545..1748
                     /locus_tag="ACG8OA_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          672 a          292 c          365 g          560 t
ORIGIN      
        1 tattttgtgt aaatagaaaa aaggaagtcc cttctgtaga atagagttac cacaacacat
       61 tcacagaaaa gaggacttcc atatgaacga ttttactaca gaaatcctaa agactctagc
      121 gaacaaaggc gatttgaatg gattattccg tgtccatttg gaaaaagctg tcaatacgct
      181 tctcaaaacg gagttaacgg ctttcctcga ttacgaaaag tacgatcgca ttggttttaa
      241 cacgggtaat tctcgtaacg gctcctatga ccgtacggtc aagaccgagt atggggaact
      301 tcatctccag attccgcgcg accgcaatgg cgagttcaag caacagactg ttcctgctta
      361 tagacggacg aatgacacat tagaggagac cgtcattcac ctcttccgaa aaggtattac
      421 catgtcggaa atcgcagact tgattgagaa aatgtatggg catcactaca cgccccaaac
      481 catgtccaac atgactaaag tgctgactga agaagtaaat gcatttaaag ttagaactct
      541 aaatgataag tatgtcgcta ttttcatgat tccttcagtt tttcagatga tataccactc
      601 aaaaaacaat atgagagatg ttcaggaata ccatctctaa tggcttataa attggtttta
      661 gggatagtag atgttataga catatcaagg aataaacgag cagctgcata aaagcagatg
      721 gaattaagga ataattgaat tgtaaaagaa gggaataaat ttaatggcac aattatttta
      781 cagatatgga acaatgaata gtggaaagag tattgagcta ctgaaagtgg ctcataatta
      841 tgaagaacaa gggaagagag ttatcataat gacaagcgca atagatactc gatcaggatt
      901 tggaaaagtt gagagtcgta taggtgtttc gaaaccagct attgcaatta cttctgaaat
      961 ggatgtgtac ggagaattag tggagattat tgatcagtac ggaaaagttg attgtatttt
     1021 ggtcgatgag tctcaatttt taagtagaga gaatattatt gatctatcac ttatcgtaat
     1081 gaatgaggaa gtaccagtaa tttgctatgg attgaagaat gattttcaaa ataatctctt
     1141 caagggatcc gaagcattat tgacatttgc tgataaaatt gaggaaatta aaactatttg
     1201 ccatttttgc aataaaaagg ctgtaatgaa tgctcgtatt gttgatggta ttccagttta
     1261 ttcaggtgaa cagattgtag taggaggtaa tgaagattac ctttcggtat gtcgcaacca
     1321 ctactacaac gaagattgaa ataaaaaacg tgtaatcaaa taattgatta cacgtttttt
     1381 attattctgt taaaggttga ggatcagaaa tttttaggta tctataatcc tcacctaaaa
     1441 atccgttaaa acgaagtaaa acatcatgac catttaaatc agttggaact aaaattggaa
     1501 ttaatttatc aacagtagtt ttgttgtaaa gttcaaaagt gttctccatt ccttctggtt
     1561 ctacaatcga atctttcaat aaacaaatca agaaatacag ccgcagaaaa gagcagtttc
     1621 aaaatgaaga atcactagag cgtttcctag tatccatctt tgatacatac aatcaaaaat
     1681 tcttaaatag aagccataaa ggcttccaac aagtgacgga tacattagct tcaatgttta
     1741 ctgagtaacc cattattttg cagaaggacg agttatttac acaaaattat tgacgctccc
     1801 tgtttggagt ggtaaaaaga ttgagaaatt gtttcattag ctattacgat tattattgta
     1861 atatattttt attgaaaatc aaaattaaa
//
LOCUS       DAZULO010000189         1878 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000189 DAZULO010000000
VERSION     DAZULO010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.195"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            178..744
                     /locus_tag="ACG8OA_002885"
     CDS             178..744
                     /locus_tag="ACG8OA_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4977608.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            889..976
                     /locus_tag="ACG8OA_002886"
     tRNA            889..976
                     /locus_tag="ACG8OA_002886"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:921..923,aa:Ser,seq:cga)
     gene            1310..>1878
                     /locus_tag="ACG8OA_002887"
     CDS             1310..>1878
                     /locus_tag="ACG8OA_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFS4977609.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT          660 a          282 c          361 g          575 t
ORIGIN      
        1 aaaatgtaag ggaacccatt gaatttaacc aaattttttt aattttgcaa aaaaaagtta
       61 aagaagactg aaaaaaaatt taaaatataa cgttttgagt aaaatgtttg caaaaaatgc
      121 taaaaatagg gtacaataaa acaacatata ctgcagtaaa gaagtggggg agttttaatg
      181 agaagaaaag tctataccaa ggatcaaatt ctaaaagctg cttatgatgt tgttgcaaag
      241 gaagggttta aaggatttac tgcgagaaat attgctaaaa agatggggat ttcgacacag
      301 ccgatttatc tggaattcaa aaacatggaa gatttaaaaa atacgttgct ggattctatc
      361 tttgaacaac taaaagaaga tgttttccct attgaacgta ctggtgataa aatcattgat
      421 ctcggtttga actatatcta ctttgcaaaa aagcatcata gtctttacat ggcattgtac
      481 attgatgaac atggggacgg aaagaaaatg catgattttt catacaatta tttccgtgaa
      541 atagttaaag aagatgaaag atacaatcaa ttatcggatg aatatgtcga tgctctgcat
      601 actggtacat gggtagcagt gactggctta gcgtcattga tgtcatcaaa catcatgcat
      661 ccaacagaag aacaaatcat tgcactgatc aaacggacaa ttgactcgat tttagcatta
      721 gataatcctg aaataatcag atagtgttct atcagtatga atttgatcgg taagtccgtt
      781 ggctgcaaac tgtcaatgga cttttttgct aaaaaaagcg atttctgtta tttatctatt
      841 agaaataggc ttgacatttc gtgaaaaaag tttaaaaata gtacttacgg aaggttggca
      901 gagtggtaat gcaccggact cgaaatccgg cgaaccggct aataccggcg cgcaggttca
      961 aatcctgtac cttccttata tcaacgatta gaggttgtga agaagctgtc ctgcatcaag
     1021 taataagaac ggccaaacaa aaatagcttt tcctattttt tcgtttgatt gggcttatta
     1081 ccgcagatgc aagcttttga acaccgtttg ttcggaataa ggggctgtga caagactttt
     1141 gtcacagccc cttattgttg tacttttaac ctacaatatt ttttgattcc ccctgagtac
     1201 tattttttat aaatgctagt aaatcaatct tctttggcct ttttttgata aaaaaaatac
     1261 tagatgtaag ataaaagtgt aaaaacgatt tgttttagga ggggaaacga tgtgtaccgc
     1321 aatcacttat gcaacgaaag accattattt tggaaggaat tttgattatg agatgtctta
     1381 taatgaagtg gtcactatta ctccaagaaa ttatcgtttc gattttagaa aggtaaaaaa
     1441 tttagataaa cattatgcaa tgataggaat agcagctggg gtatccaact atccactcta
     1501 ttatgaggct acaaatgaaa aagggttgag tatggcagga ctgaattttc ctggcaatgc
     1561 tgattataaa gaattacaag aaggaaaaga taacgttgca ccatttgagt ttattccatg
     1621 gatactaggg caatgctcaa ccatagacga agctaaagaa ttattggcta ctatcaatct
     1681 agtcaatatc gattttagtg aaaaactacc cttgtcacct ttacactggt tattagctga
     1741 taaagaaaaa tcgatagtaa ttgaaagtat gaaagatgga cttcatttat atgataatcc
     1801 tgtcggtgtg ttgacaaata atccgccatt tgattatcaa ttatttaatc tgaacaatta
     1861 tcgctcgcta tcaaatgg
//
LOCUS       DAZULO010000190         1812 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000190 DAZULO010000000
VERSION     DAZULO010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.196"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            208..804
                     /locus_tag="ACG8OA_002888"
     CDS             208..804
                     /locus_tag="ACG8OA_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS4977610.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(862..1344)
                     /locus_tag="ACG8OA_002889"
     CDS             complement(862..1344)
                     /locus_tag="ACG8OA_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977611.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1450..1647)
                     /locus_tag="ACG8OA_002890"
     CDS             complement(1450..1647)
                     /locus_tag="ACG8OA_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977612.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          613 a          364 c          276 g          559 t
ORIGIN      
        1 tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga aaatccttac
       61 ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg tgagcatatc
      121 tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa aaaaatgcaa
      181 aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt gaaaaaccgc
      241 cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt aacaactttg
      301 atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac acgcgcagtt
      361 atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc acttcgccca
      421 ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt caaaaatggt
      481 tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca agaacaattt
      541 gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat cgcaacagct
      601 aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca ttacaaccca
      661 gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa actacgttcg
      721 caattagtat tcggttcacc agaaacaccg gctggggaaa aagaatacat gaacgatgcc
      781 gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt tgtgacaaac
      841 atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct ctgcctcatc
      901 gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa atttcagcat
      961 cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac cttttggctg
     1021 atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa agctattcaa
     1081 aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa aaatcattgc
     1141 tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg tataccaaat
     1201 gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca cagtccgata
     1261 aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga ataaaaacaa
     1321 tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc cagaaaatag
     1381 gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc gcacgtccaa
     1441 ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt tttcatctaa
     1501 taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt ttgtcatttt
     1561 tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg catattgttc
     1621 tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga acttttctcc
     1681 acattaccac tgctatttct ttggagattt aactcattga tttttattta cagttaaaaa
     1741 tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac agctgttgta
     1801 tttagaagaa tt
//
LOCUS       DAZULO010000191         1799 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000191 DAZULO010000000
VERSION     DAZULO010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.197"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="ACG8OA_002891"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="ACG8OA_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS4977613.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          506 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaaaaa
//
LOCUS       DAZULO010000192         1730 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000192 DAZULO010000000
VERSION     DAZULO010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.198"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..1509)
                     /locus_tag="ACG8OA_002892"
     CDS             complement(28..1509)
                     /locus_tag="ACG8OA_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4977614.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          536 a          233 c          301 g          660 t
ORIGIN      
        1 gaataagagg atgtgacaag aattttgtca catcctcttt gttttgttct cataaccttt
       61 tttcatatac agttcatgaa ccgcttttat taacttgcca aataacactg tattgtgcgt
      121 tgtatccaga tagaaagtct ccgtttcatc tgaaattttc tcatgctgat ctgtaatgat
      181 caaatcagca tgttctgaat atgcggtaat tttaacagca tctttgcgaa ataactgctg
      241 caaatggctt tgaagatgat aatttcctgt aaactgtttg gttatctgca aatggatgag
      301 taactgctct ttcgtttctg tttgtagtaa gtgttggatc aattgactaa ggtattgact
      361 gatttggttt ctttccccat tttttgaaaa aattgtatcg attttttctc ttaatttctt
      421 ttcctgtata aaagaactag aatgattgga cgtggcttct ggaaagcaaa gtgtagaaaa
      481 actactgctg gattcaccca acagttcgaa aaaaacaact gccaacacaa gaaaataaaa
      541 ataaattggt attttatttt ctaaatggtt gagaggaaac tgttgagtta agtaagtcat
      601 caattgaccg acttgtcgac aaataaggtt tttgcttgtg gaaaataaaa aaccaatctc
      661 ctgtttttgt cgaaaagaga tttgatttgg aaaaaaacaa tagaaaaaat aattaaagta
      721 ggctctctct gtttctgtct cttcacaagg gcgactcact gaaaaagaag taaagtattc
      781 tgaataatca tgtgtatgct gcaataagtc catcaattca gtgatttcag aagaagtttt
      841 tggaaaatat gcctgttgtt tgtttcgtaa tatgaataaa gttaacagca agtagatatt
      901 tttttcttgt tttgaaattt tttctatttg ctcatctgta ctgttcattt ttagtgcatt
      961 taattcaagt gaggaaaatg gccaatcgtt acctccaaat gtatctgaaa gaaaaaagta
     1021 aagatagaga cggatagata actcatttcc ttcaagagta cagcaatttt ttacagatag
     1081 gatccgtaaa tggaattttt ttaaggaatg attgagctgt tttgtcagtt tttcgagata
     1141 ggagggcgat atataaagtt gttcaagtaa atattttttt ggtacagcag tttgtggata
     1201 attgattagt aacatcaaat agttaaaacg attggattct tttaggtagc ataattttaa
     1261 cttagaaaaa acagctaact ttttagtttg atcggtttct ttggtttgga tctctccttt
     1321 atgattagaa taaataaatt catcaatcaa taaaaattgc ttgagtgata catttatttt
     1381 tcggatcagt tctttgattg tccgttttgg aatatgtaag tcattggata attcttgtaa
     1441 agaacaatta tttttttgaa tgaccatttg tagaacaagt aactcttttt ttcttgcttc
     1501 aattagcata aatacctcct ttttattctg tttttgtata tagttagaca aaaactgtga
     1561 gtggaaagtc atttcagtct gtgttattct cacagagtgt cgccttcttt tttatataac
     1621 gttatttaat ttttttgtta ccttgtttat tctattgttt atttcctagg gtattttgtc
     1681 aagatattct tgtttttaag gcggaaaaat tgatttttaa tggatataac
//
LOCUS       DAZULO010000193         1666 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000193 DAZULO010000000
VERSION     DAZULO010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.199"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..507
                     /locus_tag="ACG8OA_002893"
     CDS             133..507
                     /locus_tag="ACG8OA_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS4977615.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(621..1250)
                     /locus_tag="ACG8OA_002894"
     CDS             complement(621..1250)
                     /locus_tag="ACG8OA_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4977616.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1384..1456)
                     /locus_tag="ACG8OA_002895"
     tRNA            complement(1384..1456)
                     /locus_tag="ACG8OA_002895"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1421..1423),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          284 g          558 t
ORIGIN      
        1 gtctacactc tgagatgtaa gaaatatcat aagatatttc ttacatcttt ttgttttgag
       61 caggacttta gataatttta ttaatatatg ctagcatgct tttgtaagaa aaagaaagag
      121 ggcgtattag ttatgtatct taaaattacg gaacaagcac aagaaagatt acaaaaatat
      181 attgatgaag gcgcaacagt tatattggat ttagatgatg gcgttgggga atattctaaa
      241 atgggtgttt gctctttaga tactagtttt cgtttacttc ttcttgataa agaacagaga
      301 aaaaatgatt ataaactcca attagatagt gatattgggg atgtttatat aaaagattat
      361 tcaaaaatgt acatggatga acatatgacc ctttctttag atccacgtct aggtgtattt
      421 actcttgaaa gcccaagtgg ttctttagac agtcatgttc aattagtaga tctacgacca
      481 gaaaaattct ctggaataaa acaataactt tctctctatt gtccataaaa ctagtctcaa
      541 tcaagctgta ttgaaaaaac aagacaacat tcttaccatg gtaaataaaa agacgacaca
      601 ttttgtcgtc tttttttaaa ttaactattt tcgataaaag atcgcatagc ttgtgccatt
      661 atttctggct tttctctatt caattcatgt tttccgtttt taataaagag tagccggcca
      721 ttcgttgaaa gtttatataa acgttttgct gctggtttat tcgcatgatc tttggtaccg
      781 cataaaatca atttaggaag tcgtgatgta cgaagctgtt ttttgcagtc taatgtttca
      841 agtgaacgaa gtagggtaag ggtttgtttt ttagatagac ctaatttttc aaatatctta
      901 gaaggtacgc agtaaaaata aatattctgg aaagtaagca ataagcgatt aggagactca
      961 aattgtggag cgactaaaac aacctttttt aaatatggag aaggagttgc aagatagttt
     1021 aaagcaataa cagcgcctaa agataatcca cataaaataa aaggttcctt tatctgattc
     1081 aattgtactt cgatgtattt ggttaagtcg tctaaagtag cagtgtctgt gctgtttaaa
     1141 tgattgaaca atgaaagact tttgcaagaa taactattgg gtagataaga gattacatct
     1201 ttccatgatt gttcgtcttg gcctagacca tgcaaaaaaa caattttcat attgctctcc
     1261 tttttatagt tatcatatag actttctttg tactattaaa atataaacat tcatttctta
     1321 cgtcataaaa taaaaaaaca gcgcagacac attgttaaac caacatgttg cactgtttaa
     1381 ggaagccact tgccgggctt gaaccggcga cctcttcctt accatggaag cgctctacca
     1441 gctgagctaa agcggcatta tcattaatac aaataatagg ataacgaata ttaatgtatt
     1501 tgtcaacaga attttttcga aaaaatgtgt ttctctaata ggaaaacaga ttttagaaaa
     1561 aataaaaata acaaaaaatg cgaacggttt ctttgataat ataataaaaa aattcaaaaa
     1621 aagtattgcg ttatttagaa gaaattgata ttatattcat gtactt
//
LOCUS       DAZULO010000194         1592 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000194 DAZULO010000000
VERSION     DAZULO010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.200"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1022
                     /locus_tag="ACG8OA_002896"
     CDS             <1..1022
                     /locus_tag="ACG8OA_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFS4977617.1"
                     /translation="KKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFMDNE
                     LTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTNGSL
                     VTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEEVDV
                     AFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLVSDI
                     NYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGNIKC
                     HSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILDEYK
                     HDILI"
     gene            1078..1296
                     /locus_tag="ACG8OA_002897"
     CDS             1078..1296
                     /locus_tag="ACG8OA_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977618.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1367..>1592
                     /locus_tag="ACG8OA_002898"
     CDS             1367..>1592
                     /locus_tag="ACG8OA_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977619.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYE"
BASE COUNT          596 a          188 c          305 g          503 t
ORIGIN      
        1 gcaaaaaact tcagatgggt gaaattgtta gaggtcctag tcaaatatct attgatgtta
       61 catataaatg taattttaga tgtaaacatt gttacaatgc gagtggtgga gacaattgtt
      121 ttatggataa tgagttgact gatgatgaac tgttagaact tacaaaagat atatgtaatt
      181 taaagccata taatatttgt ttctgtggag gagaaacttt actgagaaaa aaaatagttt
      241 tatcttgtgc cactttatta aaagaggcag gtattcctaa tattgctatt gttactaatg
      301 gatcattagt aactcaggat gttgcaaaag aattattcaa agcaggtatt aatcgatttc
      361 aagtaagttt agatggaaaa aatgctagta gccatgaagc acttagatta tatccaaaca
      421 gttttgaaaa ggcaattaat gctatcaaat tatttagaga attgtccgaa gaagtggatg
      481 ttgctttttg tcctactagt tttaattaca atcaattacg agaagtatat caactagtaa
      541 atactcttaa agtgaagtct ttaagaattc aaccatttat gcttagtggt aggggaatgc
      601 aaaataggga aatagtgcca actgtttctc aatatagaaa actagtttct gatattaact
      661 atattaaaag aaatgaagat tataccaaga tagattgggg agatccaatt gatcatatca
      721 tccgacacag aactttattg aaagattttt cgaattttgt ttctattagg tcagacggag
      781 gattaatggc atcaccttac ttgccaattc aagttggaaa tattaaatgt cacagtcttt
      841 ctaaatattg gcgagcagga ctttataaag ttttttcttt agatttagtt caagatatag
      901 ctactcaagt ttcttcaata caagatatga gtaaagaatt aaaaaaagga catattaaat
      961 ggcttaatga agatatagaa attgatattc tcgatgaata caaacatgat attttaattt
     1021 aggtttatca ttggaaagtt tagataaaca aagtactttt agaaaggagg gaaaaaaatg
     1081 atttctgaac ctcaaatgaa taatatgtat gaaagcccaa ctataatggg agctggtgaa
     1141 gctgcgccaa gagcttgttc agcaattatg gtgtgtgtgt tattagcatc tgcaggagta
     1201 ggtatccaag caggagcggt gtatacttat gctgtagttg ctacagtagc tgtagctgca
     1261 gccgcagttt ggaaaacagt aggattagtt ggttaggaat taataggaga ctaactgtct
     1321 cctattgatt ccaatgatta agctaaggag gaattttaag aaaataatga aatacaataa
     1381 tttaaaaccg aaattactta atgagaacat agaatatata agagaagaaa attatggata
     1441 tttaaccata gttcccaaaa atggaactga attgaaagat aacgttttca atccaacagt
     1501 aagatatata ttggctaact gtactggaaa aaacagtgta aaaaaaatag cgaatggttt
     1561 aaaaaagaag tttcctagtt tagaatatga aa
//
LOCUS       DAZULO010000195         1577 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000195 DAZULO010000000
VERSION     DAZULO010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.201"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(141..1433)
                     /locus_tag="ACG8OA_002899"
     CDS             complement(141..1433)
                     /locus_tag="ACG8OA_002899"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFS4977620.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          444 a          338 c          273 g          522 t
ORIGIN      
        1 ttcggaaaaa gggactgtga cacggttttt gtcacagtcc cttattctga ataaacagtg
       61 ttcaagttat tattatcgta tagataaaca cttcgactcg ctctggtaag aacgtcctga
      121 ttcagtcgtc gtttttatcc ttagatttgc gcccagacgc tttctaagat gtttgtttgt
      181 gttctatcag gtccgacaga aaatgttgag atacgtacac caacaagctc tgagatacga
      241 cgtacatagt tacgtgcgtt ttctggcagt tctgcaagtg ttttgcatcc tgtaatgtct
      301 tctgaccagc ctggcaattc ttcatagatt ggtttgcagc ggttcaactc tttcaagctt
      361 gctggatagt ggtagattag ttcgccatct aattcataag ccgtacaaat ttttactgtt
      421 tccaatccac ttaatacatc gatcgagttt aaagataggt tggtaatacc tgaaactcgt
      481 ttagaatggc gcataactac agaatcaaac cagcctacac gacgcggacg accagttgtc
      541 gtaccgtatt ctcgtcctac ttcgcggatt tgttctcctg tttcatcaaa taattcagtt
      601 ggaaatggtc catcaccgac acgtgacgta taggctttgc agacgccgac aactttattg
      661 atttttgaag gccctacacc gcttccgatt gttaccccac cggctactgg gttagaagaa
      721 gtaacaaaag gataagttcc ctggtcgata tccagcatta caccttgtgc gccttcaaac
      781 aacacgcgtt tgcctgcatc caaagcatca ttcaagataa cggaagtatc tgtgacgtat
      841 tgtttgattt gttgtccgta ttcataatat tcttcaaaaa tatcgtcaaa atcaagtggt
      901 tctgaatcaa acatttttac aaattgacgg tttttgtcat ctagattcaa acgaagacgt
      961 tcttcaaaaa tttctttatc taataagtct gcaatacgga tacctacacg agctgcttta
     1021 tccatatagg caggtccgat tcctttaatc gttgtaccaa tcttattttc gcccttagca
     1081 tcttcttgta attggtccaa tttgatatga taaggcaaga ttacgtgcgc gcggtcagaa
     1141 atacgcaaat tacttgtttc tacacctttt tcatgtaaat aagctaattc tttaaccaat
     1201 gatttaggat tgactacaac gccgtttccg atcacactga tcttgtcttt ataaaaaatc
     1261 ccagaaggaa tcaaatgcag tttataagtc acaccgttaa acttaattgt gtgccctgca
     1321 ttgtcgccac cttggtaccg tgcaatcact tcagcatttt cactgagaaa gtctgtgatt
     1381 tttcctttcc cttcatcgcc ccattgcgta cctactacta ctacagatga catgtgaaca
     1441 cctcgttcta tattatttaa tcctgaacca ttgtatcaat aatagataca tatttcaaat
     1501 aaaatcgaat gatttttatt tttattttat aaaaacgctt aaaaaacgaa ctttatcttt
     1561 ttagaatggt tattaaa
//
LOCUS       DAZULO010000196         1573 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000196 DAZULO010000000
VERSION     DAZULO010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.202"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          594 a          179 c          320 g          480 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caaaggagtg taa
//
LOCUS       DAZULO010000197         1554 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000197 DAZULO010000000
VERSION     DAZULO010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.203"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..1510
                     /locus_tag="ACG8OA_002900"
     CDS             44..1510
                     /locus_tag="ACG8OA_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFS4977621.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          430 a          290 c          327 g          507 t
ORIGIN      
        1 tgtataatat tttctgaaaa tttaaagaat tggggtatag attatggaaa aacagcaaga
       61 caagggcttc ttcgggcagc cgaagggatt gtcaactttg ttcttcactg agatgtggga
      121 acgatttagt tattacggta tgcgtgcatt acttatctat tatatgtacg atacagttgc
      181 caacggggga cttggattac cacgagcaac agcattagct atcatgtcga tctacggatc
      241 tttgatctac atgtctagta tcgtcggcgg atgggtatct gaccgagtgt taggagcaaa
      301 acgcactgta ttcttcggtg gagttttcat tatgcttggc cacatcgtct tggctttacc
      361 atttggtgta agtacattgt ttgtttcgat ggcattgatt attatcggaa ccgccttttt
      421 gaaaccaaat gtttctggca tggtcggaca tctttattca aagactgact tgcgaagaga
      481 tgcaggattt tcgatttttt acatgggtgt caacttaggg gcattattag caccactaat
      541 cgttggtaca atcggtcaag aatataacta tcatttaggg ttctctttag ctgctattgg
      601 gatgttcttt ggattattac aatacatcat ccaaggacgc aagacattag acggagtggg
      661 tacaacaccg ccgaatcctt tgacaactga agaacgtaaa ggattcatcc gtaacttgat
      721 catcgcactt gttgttatcg ctgcagtatt cggcggggca caactaactg gtcatctgac
      781 aattgacttt tttgtcaata caatcagtgt attggggatt atcttaccgt tgtattactt
      841 tatcaaaatg ctgacttcaa aagatgtaac tgctgaagaa aaaccaagag tatgggctta
      901 tatcccatta ttccttgcag caatcatttt ctggtcatta caagaacaag gttcatctat
      961 cttagcttta tttgctagtg aacgtacaca atctacgcta tttggtgtaa cgatccaagc
     1021 aagctggtat caatcattga atccaatttt catctttatt ctgacaccag tattcgtaac
     1081 gatgtggacg aaacttggtg aacgtcaacc atctacagta gtgaaattct ctcttggact
     1141 tttatttgca ggattatcgt acttgctatt gatgttgccg ggtatgcttt atggtacaag
     1201 tggaaaagta agtccattgt ggttgttggg aagtttcttc atcgtggtta ttgctgaatt
     1261 gtgtctttca ccagttggtt tgtctgtgac aacaaaatta gcaccacgag catttgaatc
     1321 acaaacaatc gctatttggt tcttagctga tgcttcttca caagcaatca atgcacaaat
     1381 tgcacgcttc tacacaccgg gaacagaatc tgcttacttc ggtattatcg gtgcagtagc
     1441 aatcgtcggt ggtatcttgc tatttactgt gaaagaacca atcaaaaaac taatgggctc
     1501 tattcattaa tcaatgaaca taaaaacagg aattgcttat ttgcagcagt tcct
//
LOCUS       DAZULO010000198         1551 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000198 DAZULO010000000
VERSION     DAZULO010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.204"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..344
                     /locus_tag="ACG8OA_002901"
     CDS             132..344
                     /locus_tag="ACG8OA_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977622.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="ACG8OA_002902"
     CDS             506..1024
                     /locus_tag="ACG8OA_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFS4977623.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="ACG8OA_002903"
     CDS             1031..1543
                     /locus_tag="ACG8OA_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977624.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
BASE COUNT          611 a          181 c          241 g          518 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt a
//
LOCUS       DAZULO010000199         1516 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000199 DAZULO010000000
VERSION     DAZULO010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.205"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..888
                     /locus_tag="ACG8OA_002904"
     CDS             <1..888
                     /locus_tag="ACG8OA_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFS4977625.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
     gene            complement(1187..1378)
                     /locus_tag="ACG8OA_002905"
     CDS             complement(1187..1378)
                     /locus_tag="ACG8OA_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977626.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT          590 a          238 c          234 g          454 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc aagaaccggg ggctttattc ctgcagtccg gctacgcctt cctgcaatag
      961 acaaaactcc agatcgggta attcgcttgt ggcgaaaccc gacctggagt ttgtctctgt
     1021 attttgaacc ctaaaaatta atcaaacaga ctgatattct cataatcagc gcgaaataag
     1081 ccattaagta cagttttcct acctattttt gataacccgc tggttagtta atattccttt
     1141 tccgagagag ataaatgatt ggttaaatct atatacatct caacctttaa aaatgttttc
     1201 gaaatacttc atcagtacat gtcatacaaa ttaccagtgc attctcaaat tctagttctt
     1261 taattgattt cagttgagga gcagttactt ttggcggaaa tttccatttt tgtccaaacc
     1321 tcttcccccg atgaaatttc tttgttgcaa ttattgcaat gaaaaatttt tgtttcaaaa
     1381 atatacataa ttacttcctc caataaaaac tatctacctc tcaaattatt tttctctaat
     1441 aagaaaaaca gaaaaggtaa taagatacaa cagtgatgat tcctaataaa gaaaagacta
     1501 caataatatt tttcta
//
LOCUS       DAZULO010000200         1490 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000200 DAZULO010000000
VERSION     DAZULO010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.207"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..221
                     /locus_tag="ACG8OA_002906"
     CDS             <1..221
                     /locus_tag="ACG8OA_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFS4977627.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            218..1237
                     /locus_tag="ACG8OA_002907"
     CDS             218..1237
                     /locus_tag="ACG8OA_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS4977628.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
BASE COUNT          486 a          281 c          310 g          413 t
ORIGIN      
        1 ataataaaac gttcgaagta ctaaaatggt tcgcactggt aattattccc gcactagcta
       61 ctttcgtggg gttagttggt aaagcgctca attggcagta cacagatatc tgtgttgtca
      121 tcattactgg ttttggcgcg tttttaggga gtgtgttggg tgtatcaaat cgaacctaca
      181 aaatgttctc ggctgaaagc gaagaaggag gaaacaaatg aaaaagaaaa ttactattac
      241 tgcgatgagc ctgttaacgg ctcttttttt attgccaatt aacggatttg cctatactat
      301 caacaatgaa tttaatttgg gcgcaaatga aggtagctca caagtagcaa ataatcagta
      361 tattttactg catgaaacgg ctaatgaaac agcaacagga cgcaatgaag cgcagtatat
      421 gcaacgttca tggactagcg cttatactgc ttatattgtg ggagacggcg gaattgttta
      481 ccaagtcgga caacctggtt atgtacagta cggtgctggt tcatatgcta atgccaatag
      541 tcctgtgcag attgagttac aacacacaca tgataaagca acttttgaaa aaaactataa
      601 agcatacgtt gaattggcta gagattcagc aatgaaatat ggtattccat taacattaga
      661 cacgccttat aaccaaccag gaatcaaatc acatttatgg gtaactcaaa acatttgggg
      721 cgatcacact gatccgtatg gatacttatc agaaatgggt gtaagtaagg aaaaactggc
      781 ttacgattta gctcatggat ttacagacga aaacccaacg acttctgaga acaagcctgt
      841 cattgatcca acccgagcag gtgcagcaaa tccaacttta tcagatggaa cgaaccattc
      901 tcacattgat cagtttggag aaatcgaaaa tgcgaactta cacgtcgctg gatggcacat
      961 cgctaactat aagtacgagt atattttcat tatggactac aatactggga aagaattagc
     1021 tcgagtaaga gctgatggaa tttatagacc ggatgtaaat caagcttata atacttctgg
     1081 aaatgttggc tatcatgtat cttttaacat gcgtaacttc cctaataaga aagtctatgt
     1141 catgatgcgg gcaacgaatg atccagaggg aaacactaaa ggcggtgcgc aagatttcca
     1201 tgacaaacgt tggtatttaa atattcctaa acgataaaaa tagctcctcg ttgaggagca
     1261 ttacataact atattgacaa ctataaaaat cattcgataa aatagtgatg ttatcgcata
     1321 tcttcactat cacccataat agtcacactc caagctatgc gataacaggt ttgttgccac
     1381 acattctact ggttgattgt ttatggcttt atgtggcaac aaccagtacc cttagctcag
     1441 ttggtcagag cagacggctc ataaccgtcc ggtcgtaggt tcgagtccta
//
LOCUS       DAZULO010000201         1489 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000201 DAZULO010000000
VERSION     DAZULO010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.208"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1100
                     /locus_tag="ACG8OA_002908"
                     /note="aph(2'')-Ia"
     CDS             <1..1100
                     /locus_tag="ACG8OA_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HFS4977629.1"
                     /translation="RYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIED
                     LPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVA
                     YLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGY
                     KEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEY
                     ILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTG
                     IIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYY
                     PIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1230..>1489)
                     /locus_tag="ACG8OA_002909"
     CDS             complement(1230..>1489)
                     /locus_tag="ACG8OA_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4977630.1"
                     /translation="KYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRR
                     REKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          596 a          139 c          252 g          502 t
ORIGIN      
        1 caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct aatgcagtta
       61 ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa tctggtttta
      121 gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa gattgttatt
      181 taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa tatttaattg
      241 agcattactt tgataatttc aaagtagata gtattgaaat aatcggtagt ggttatgata
      301 gtgtggcata tttagttaat aatgaataca tttttaaaac aaaatttagt actaataaga
      361 aaaaaggtta tgcaaaagaa aaagcaatat ataatttttt aaatacaaat ttagaaacta
      421 atgtaaaaat tcctaatatt gaatattcgt atattagtga tgaattatct atactaggtt
      481 ataaagaaat taaaggaact tttttaacac cagaaattta ttctactatg tcagaagaag
      541 aacaaaattt gttaaaacga gatattgcca gttttttaag acaaatgcac ggtttagatt
      601 atacagatat tagtgaatgt actattgata ataaacaaaa tgtattagaa gagtatatat
      661 tgttgcgtga aactatttat aatgatttaa ctgatataga aaaagattat atagaaagtt
      721 ttatggaaag actaaatgca acaacagttt ttgagggtaa aaagtgttta tgccataatg
      781 attttagttg taatcatcta ttgttagatg gcaataatag attaactgga ataattgatt
      841 ttggagattc tggaattata gatgaatatt gtgattttat atacttactt gaagatagtg
      901 aagaagaaat aggaacaaat tttggagaag atatattaag aatgtatgga aatatagata
      961 ttgagaaagc aaaagaatat caagatatag ttgaagaata ttatcctatt gaaactattg
     1021 tttatggaat taaaaatatt aaacaggaat ttatcgaaaa tggtagaaaa gaaatttata
     1081 aaaggactta taaagattga ttatataata tatgaaaagc tattataaaa gacattagta
     1141 ttaaatagtt taaaaaaatg aaaaataata aaggaagtga gtcaattcca gactcctgtg
     1201 taaaatgcta tacaatgttt ttaccatttc tacttatcaa aattgatgta ttttcttgaa
     1261 gaataaatcc attcatcatg taggtccata agaacggctc caattaagcg attggctgat
     1321 gtttgattgg ggaagatgcg aataatcttt tctcttctgc gtacttcttg attcagtcgt
     1381 tcaattagat tggtactctt tagtcgattg tgggaatttc cttgtacggt atattgaaag
     1441 gcgtcttcga atccatcatc caatgatgcg caagcttttg aatattttg
//
LOCUS       DAZULO010000202         1417 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000202 DAZULO010000000
VERSION     DAZULO010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.209"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG8OA_002910"
     CDS             complement(150..1337)
                     /locus_tag="ACG8OA_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFS4977631.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAZULO010000203         1378 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000203 DAZULO010000000
VERSION     DAZULO010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.210"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..469)
                     /locus_tag="ACG8OA_002911"
     CDS             complement(170..469)
                     /locus_tag="ACG8OA_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS4977632.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            820..>1378
                     /locus_tag="ACG8OA_002912"
     CDS             820..>1378
                     /locus_tag="ACG8OA_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS4977633.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEH"
BASE COUNT          462 a          220 c          195 g          501 t
ORIGIN      
        1 agtttatact agctgttttt gtgttaatta aaggataaaa tacaatataa agaaacatct
       61 gtttctttat ttagaataaa aaaatacaga ctctgttcaa ataaatgaac ctagcctgca
      121 ttatgagatt gactatactt tttaattcat tgagatgaat aactcgtttt tataaatgcc
      181 caaaagtaat cggtgccgaa gccattcctc gacctaattc tttttcaaat tttcctattg
      241 ttgatatctc catataaaac ggtgatactg gtttagacaa ttctcccctg attgctaacg
      301 gacgtaaact acgttccaaa ttatttatga ctttaggata atactgacct ttttctaagc
      361 ctgtctttgc gtctatcaaa agtatacgtt ccttttcttt gatctctgga tttaaaacga
      421 gatcatataa ttttagtacc atgttttcta tttcttcact tttagacatc acgatttcct
      481 cccaaaaata tatttcttcc cactacattc tataaacaaa gtatacgcta catctagatt
      541 tttttaaatc attactggat tgttaaaacc tttcaataca ctgttatggc aacgattttt
      601 attttaaaaa cattctaaat taaatcagta aaaattttta ttctaagatt tttattagaa
      661 ttacgcctaa tttgtgtctc tcagggcaat tctaagaacg tttcaagaga tagcgacact
      721 atcgttatcg aaaaaggcga aaacctatct cattttctgc taatgtaagt tggtttagct
      781 tttgttaata tgactttatg aaaatggagg taattaaata tgatttttcc taaagttaat
      841 gaagcaagct tgaataaagg atcctttttt catatctatg aaaacgaaga ttttgaactg
      901 tataatatcc tttcacaaaa agaggtcatc gaaaaccaaa taatctgcca tccaattatt
      961 tacaaatcta ttttcaagta tgcatttttt gcttccaatg attttcaaag aatggagatt
     1021 accgactgta tattcgaaaa ctgtgatttt tcaggctgta tattgacaaa aagcatgttg
     1081 catcgcgtga aatttgtcaa ttgtaaatta acaggtacta aattgatgga aagttatatt
     1141 gggaacacgt tattcgagaa ttgtaaaatg gactatgtaa atttatctgg ttcaaacata
     1201 aaagaaagta cctttgagca ctctgtctta tcaagcgcag attttgtaga ctgttccctc
     1261 actaaaataa tgttttctac taatgattta gaaaatgcta gcttttttga tactgattta
     1321 aaaaatatcg atttaagtag caactccttt gaaatgattg aagtacgctc ggaacatc
//
LOCUS       DAZULO010000204         1362 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000204 DAZULO010000000
VERSION     DAZULO010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.211"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1362
                     /locus_tag="ACG8OA_002913"
     CDS             82..>1362
                     /locus_tag="ACG8OA_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFS4977634.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          379 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DAZULO010000205         1362 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000205 DAZULO010000000
VERSION     DAZULO010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.212"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..1349
                     /locus_tag="ACG8OA_002914"
     CDS             414..1349
                     /locus_tag="ACG8OA_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977635.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          509 a          164 c          241 g          448 t
ORIGIN      
        1 gatacaatca ttagtctaga gaaaaattaa gaaccgtagg aactacgggg atcgcttggt
       61 gaataagaga cactgctatt agtgaaaaaa taagatatca agtacgctct attcccaaaa
      121 atttcgtgac tttactcatg agaggttcaa gtagtatatt tttaatcaaa attgacgtaa
      181 cttttaataa atactaccta aataaggtag tatttatttt tgtgttttaa aagaggaagt
      241 ccaatatatc tatgggaaac ttcttaaata ttatgttggt acatgaaaat atacatccct
      301 gctagactat ttacaacatt ttactttgta cgtattcttg ttttaaaagg aatgggataa
      361 aatataggta caataactat ttgatgctaa atataattaa tggaggagag atagtgaaca
      421 tacttaatga aaaaagatat gtatggtttg ctggtaaaaa atatattact actttcaaaa
      481 gattatccga gttaatgcag cagactgtta aaagtgctat gcctgaagga tacaagttta
      541 attttgttca atcttcagta tctactgaaa gtatctattt ttcaattctt tctgagagta
      601 ggctttttca ttttagaata agttctcatg taaagaaaac tgatgttgat atatatacgt
      661 tccgtacaaa taattataaa aatttaaatg atttgagtag aagtatcagt gaatatctca
      721 aggggaatag ctatctcaag attcgatttt cacatatatt aattttacta ccatatttga
      781 atgagaattt caccctaatt caagtagata agtctttcat tgcctcaggt aatcaaggaa
      841 atatgaatat tacaagtaac aaaacgtcac gtgtgctatt ggcgttgatg ggttacggaa
      901 ttctttatag agatgaccaa aatggccaga ttctaataaa tcgggctggg cgttcattat
      961 taatgaaata tagaaaattt ataccggaag attttcaaaa cttacagtta gaaaacatgc
     1021 gtttagaaga tttatttaga ttagcagaag atgataagta tagatttgaa gttttgaaaa
     1081 ttcaaaagaa tgaactattg aataagttat atcctgagaa tgcgataaaa gcacattttg
     1141 gtgatgctaa tcatatgtat agttggttca ttcctaaaag atttagaggg aaaatcaatg
     1201 ctggagatca agtgaaagta ctcaatcgaa atagtacaga tgtcgtaatg ataacagata
     1261 tctatgtaga agatgaagat ataatcaaat atatgaagcc tgtgataaaa cttgttaata
     1321 aaaataaagg aacccgtggt gctagttaat tttttcctag ag
//
LOCUS       DAZULO010000206         1355 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000206 DAZULO010000000
VERSION     DAZULO010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.213"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1355
                     /locus_tag="ACG8OA_002915"
     rRNA            <1..1355
                     /locus_tag="ACG8OA_002915"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAZULO010000207         1324 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000207 DAZULO010000000
VERSION     DAZULO010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.215"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..820
                     /locus_tag="ACG8OA_002916"
     CDS             278..820
                     /locus_tag="ACG8OA_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977636.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..>1324
                     /locus_tag="ACG8OA_002917"
     CDS             976..>1324
                     /locus_tag="ACG8OA_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFS4977637.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          533 a          157 c          248 g          386 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccag
//
LOCUS       DAZULO010000208         1307 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000208 DAZULO010000000
VERSION     DAZULO010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.216"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            217..>1307
                     /locus_tag="ACG8OA_002918"
     CDS             217..>1307
                     /locus_tag="ACG8OA_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977638.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTG"
BASE COUNT          433 a          131 c          227 g          516 t
ORIGIN      
        1 gtttctttga aatttctata tacaatgaga agctgaaatt tacttttatt tgatgactgg
       61 aaatagaaat taaatatatt gtattgttac attaaagaaa acttgtatgg aaaaatcagt
      121 acaataccat tatttaatgt tcttcactta aagcgcaaaa aatatcagtt tattaaactc
      181 gtatatagaa caaatatatg tagacggggc gaaacgatga gtgtaaaaaa aatgctattt
      241 atggtaagta caataattgt attaacatcc cgattttggg gaattaatac tagtttatct
      301 atgcgatatt ttattatggc aatatgggat ttatatttta tgttaagcta tttttcttat
      361 cgtgagaaaa catataaaaa atacgttatc aagaataact tttggttgac tataatgccg
      421 tttgtgatat tttctatcta tactctaata atatggggaa ttggttcaaa tgcagaattt
      481 gaaaatttta taaagttatg ttccacgttg ctttatttaa ttcttgcata tggttacgca
      541 tgtacagcat tttacttatt taaggcagaa ggaattgatt ttattttttg gtcaggtgtt
      601 atttcatata tgagtggttc agttttttat ttaattgtat cttatggttt aaatggttta
      661 atttcttata taaaaagtct tataacaggt gaaacaaata ctgcaaattt tgctatggag
      721 gtacatgatt taacttttgc gatgggggtt tttttccttt attacgtatt ttttgaaaat
      781 aaaaatacaa aaaaacataa gataaaaatt atcgtatctt taattttaat tttcatggga
      841 ttgaagagaa ttgaactgtt agctttaagt ggtgcaattc tagtgtatta tgttttatta
      901 aagtggggaa aaaagattca aatccgtgca ttagtgtgta ctatttgtgc aactgttatt
      961 tcgttagtgt ttatttacat tatagataca ggaattttgg cattgcttat gtcaaatttg
     1021 ggcattacag atgacggtgc gaggctttca ttttatctat attcatctaa attttatgaa
     1081 cttggtttag gatatgtagg aacaggatgg acatggtttt caaaatactt ttttaatctt
     1141 tatttatcgc actttaggtt aaatggtgga agaattgcag caagcctaca cagtgatata
     1201 ttaactattt atatagaagt tggatgtata gtttttatta tatggatgtt ttggttattt
     1261 tttgtgaaac cgataagact aactaaaaaa tttggattga aaacagg
//
LOCUS       DAZULO010000209         1294 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000209 DAZULO010000000
VERSION     DAZULO010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.217"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1244
                     /locus_tag="ACG8OA_002919"
     CDS             <1..1244
                     /locus_tag="ACG8OA_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFS4977639.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAZULO010000210         1269 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000210 DAZULO010000000
VERSION     DAZULO010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.218"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..230
                     /locus_tag="ACG8OA_002920"
     CDS             <1..230
                     /locus_tag="ACG8OA_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFS4977640.1"
                     /translation="PEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDIDS
                     EEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            235..1182
                     /locus_tag="ACG8OA_002921"
     CDS             235..1182
                     /locus_tag="ACG8OA_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS4977641.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          415 a          182 c          264 g          408 t
ORIGIN      
        1 cacctgaagt ttttgaaatg aatgcctcaa tgtattcttt taaacctgaa tttctaaaaa
       61 aaagtaatta tatttttgat gggcaatgtg ggataataaa aatgagagat tatctaattt
      121 tagatattga tagtgaagaa gattttaaat ggttagaatt tttgtttcct aaatttttag
      181 aagattcttc ggagcttcaa gatatctata attatttata tcggaggtaa aaggatgagc
      241 tgttatctta tcaccggtgg ggctggcttc attggttcta ccttagccaa tcatttagga
      301 gaagaacatg tggtaatcgt cgtcgatgac ttatcaatgg gaaaagtaga aaatcttgat
      361 atgacgaaaa atatcacttt tattgaggga gatgtcgctg agaaatcctt gatggaagag
      421 atcatgaaag ctaataaatt tgattatatc tttcatttag cagcagttgc tagtgtggca
      481 gattcagttg cccgaccagt agaaacacat cgtgtgaatt ttgaaagtgt attaatgatt
      541 ttagaattga tccgcaagta tcaacctgat ttaaaacgga tcgtgtttag ttcttcagcg
      601 gctgtttatg gggatgaacc gactttacca aaaaaagaag agtccgtgat tcgcccattg
      661 acgccttatg cggttgataa attcgctgca gaacaatatg tgctagatta ttgtcattta
      721 tatgatgtac ccggtagcgc agttcgtttt ttcaatgtct atggtccaaa tcaaaatccg
      781 aattctccat acagtggtgt gatttctatt ctcgtagatc gctataagaa acaaatcgta
      841 ggagaagcaa cttcatttac attatttggt gatggaagcc agtctagaga ctttgtttat
      901 atagatgatg taatccaagc attactttta gttgcgaatg aggaagctgc actggggaaa
      961 caattcaatg ttggaacggg taaagcaacc acacttttgg aattgattca tgcaatcaat
     1021 gaaattctcg gtgtagaatt gacacttgaa tacaaagaag agcgttcggg tgatatccat
     1081 gactctctag cagatattac taagatccag tcaattggct atcaaccgaa atatggtatt
     1141 ttaagtggaa tgactaatta tttgaaaact gaaatcaatt aaaacatact aagataagat
     1201 tagagggtaa attcaggctc tttgtcaata aggactgatg agttttgtaa aatcaaatct
     1261 gggatagaa
//
LOCUS       DAZULO010000211         1265 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000211 DAZULO010000000
VERSION     DAZULO010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.219"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..1099
                     /locus_tag="ACG8OA_002922"
     CDS             20..1099
                     /locus_tag="ACG8OA_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4977642.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT          376 a          301 c          310 g          278 t
ORIGIN      
        1 tagacaagga tgaattcaca tgacgcaaac ccatgataac acagtttcac gcaagggaaa
       61 acatctttcc tactcagaac gttgccaaat cgctatcctg aaaaaggaag gctattccaa
      121 ccgtcaggtt gccaaagtgc tgggacgtgt tccgcaaacc ataaacaatg agattgatcg
      181 cggaaccatc acccagttaa aacgccaaac gcaaaatgga aaagtgtatg acacctacta
      241 ttctgtttat gacgctgatg caggacaggc cttctatgaa aagcaacgac tgaactgtgg
      301 cagacgcccc aaatgggcga acacaaatgc cttcattgat tgggcagatg acaaaatgtt
      361 aaatgaaaaa tggtcgccgg acgtcgtggt cggatttgcc agaaaacacg agctgttcga
      421 ttgtgccatc attcctggca caaccgctct ttatcaatgg attgaccgcg gaatcatgcg
      481 aacaaagaac atggacttgc ttgaaaaact gtcccgtaaa atcaaagcgg tccgcccagg
      541 acaccgtccc aacaagcgtg ttctcgggac gtcaattgaa gaacgcccta aagaagttga
      601 aacacgggaa acgttcggtc attgggaaat cgacacggta gtgggaaaca agaccaagtc
      661 ggatgccgtg ctgctgacgc tggctgaacg gcagacacgc tttgaagtca tcctaaagtt
      721 gaacgggaag gacaccgaat cggtggacca agcggttcaa cagctaagag aacgggctgg
      781 cgctaacttc gatcaattat ttaagaccgt tacatccgac aatggctcgg agttttctgg
      841 gctacacgat gctctgcaag atgtagctga cgtctatttc agccacccct atgcttcttg
      901 ggagcgtggg acgagcgaaa accagcacaa attaattcgc cgtttcgtcc ccaagggaag
      961 cccgattagt gaggtgagcg agaaccagat tttgcggatc cagcgttgga tgaacgatta
     1021 cccgcggaaa attttgggtt atgcgacacc acatgataca ttcgtccagg cattcaagca
     1081 agaacggttg gtggcttgac ccattattcc ttgataacga tcctcactcg ggacatggct
     1141 tcatgtctgg aaagctggcc tggggccaga atcgtctgaa gactatcata cctcagttca
     1201 ttatcaaggc ctcactgatt tagaataacg aggcagttac ggtatcaaac aaatgagtta
     1261 cctgt
//
LOCUS       DAZULO010000212         1249 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000212 DAZULO010000000
VERSION     DAZULO010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.220"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..>1249
                     /locus_tag="ACG8OA_002923"
     CDS             39..>1249
                     /locus_tag="ACG8OA_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4977643.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKR"
BASE COUNT          402 a          255 c          234 g          358 t
ORIGIN      
        1 aattgaatca ccacaacaca atcaaaggag cgttctgtat gcctgatctt catcatatca
       61 aactactact tggaatcaaa gataaaaaca ttttcatcac aaatgtggaa tcgaagagca
      121 ttaagaatat caagagtctt gttgtttctg cgacgttaag taaagaaatt cgtcgctgtc
      181 ctttgtgtaa acagatgaat catgaaggca tgatcgttaa gaacggaaag aaaaaatcgc
      241 tcattcaact taataaatgt gcgaaccaac tgacttatct tgctttagcg aaacaacgct
      301 accactgtcg tggctgccac acctatttta cggcgaatac ctacattgtc gatagaaact
      361 gctttatcgc taaacaagtg cgctataaaa ttttagaaga gctaactgaa aaacaagcaa
      421 tgacgacaat tgctaagcat tgcggcgttt cttggtcgac cgtttctcgg acactcgctt
      481 ctctgatccc catgacaaaa gtaaaaagaa attggttacc gcgttgttta ttaatggatg
      541 aatttcgttc actgaaaaat caagtcggtc cctactcctt ttcttgtatg gatggcgata
      601 ctggaaaatt actggatatt cttccttcac ggaagaaaaa ggctctcgtt tcttatttta
      661 tgcaatttga acgacgcgca cgattgaagg taaaggtcat cgtgacggac atgaatgttt
      721 cttatgcctc tctgataaag gaatgttttc caaaggcaaa actcgttgtg gatcgttttc
      781 acattgtcaa acatctcatt cgtaaatttg aggatattcg cgtacgaatc atgaaaagct
      841 ttgatcgaaa cgatccaaca caagccaaat attatcgaca attaaaggcg ttgtctcggc
      901 ttctgatcaa acgacaggac aagcttgttt atgacaaatg gaccaaatgg cgcaattttg
      961 gttgggcgta tctcaccgaa agtgaagtgg tggatcgtct gctttcgatc tctgacgaat
     1021 tacgaatcgc ctatttttac tatcaagaaa ttcttcaagc gtttcacgac aaagaggcgg
     1081 acactttctt taaacttgta agaacgatgc ccaattccgt tccaaaagaa ctgcattcta
     1141 taaaaaaagc ttttataaac tatgaatccg gcattcgttt agccctcgaa ttgccttatt
     1201 caaatgccaa aatagaaaat ctgcataccc acatcaaagc attgaaacg
//
LOCUS       DAZULO010000213         1193 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000213 DAZULO010000000
VERSION     DAZULO010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.222"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACG8OA_002924"
     CDS             <1..1076
                     /locus_tag="ACG8OA_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS4977644.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DAZULO010000214         1189 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000214 DAZULO010000000
VERSION     DAZULO010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.223"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..>1189)
                     /locus_tag="ACG8OA_002925"
     CDS             complement(85..>1189)
                     /locus_tag="ACG8OA_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFS4977645.1"
                     /translation="FPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISY
                     HVPTGTPGLLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFY
                     MMNFIVQIFYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSIL
                     LVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEI
                     SAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMH
                     NGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEM
                     VEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          462 a          196 c          146 g          385 t
ORIGIN      
        1 tttgattctc ggtaaaaatt ttgataaaca tttaatatat taatcaaatg taaaggagtc
       61 caagcaatga aagctgtctt catattaatt ctcctttttt aaatacaagt aagaaatgct
      121 tcccaaataa taccagaaaa caattgaaat gacatcgcgc ttattgaaaa gtacatgagc
      181 ctccaccatt tcatctataa aaatagatag tataaaaatt aaaacaatac caagaaacgc
      241 ataattcact ttacttctaa aaaatgtttt gagtaaacta aagatattta acacatagaa
      301 agcatagata catataaaag caattatacc acttgataca agtacttgaa gaaatccatt
      361 atgcatgttt ccatgtgctc tttttagttc attcacatct gtagtatcaa gatctacttt
      421 tttcaaaaat tttgcatcgc tattcccata gaaatcggtc tctccataac caaataacaa
      481 agttggtctt ataactttaa gcgcagcttt ccaaatcgta aaacgaccag ctgaaatttc
      541 ggagttatca ctagaatgct gtaatattac ttctttttct ggaagtggag ttttatctga
      601 ttcatttgac tctataactt ctacttttct atcaatcgat acaggaattg ttgctaaaaa
      661 atccttagca taattagtac caaaagaaaa atataccaat agaactaata atatgcttac
      721 catccatttg atttttacag gtagtacttc tctaatgctc tttctaaatt ttgaattggc
      781 aaacaacaaa agaaataata ctaaaaaaga acaaattact acctgggcac ctctagacga
      841 agaaagtgta atataaaata tttgtacgat aaaattcatc atataaaaaa cacgaatcgt
      901 ttttgttatt tttgaaatta atacactaat taaacttaaa ataatagaag tatatccaaa
      961 catagctcca acatttggac ttgtatagac accaaataac cgatgttcaa taaaaccttg
     1021 tcttgcaagt aatcctggcg ttcctgttgg gacatgataa gaaatcaaaa aaataaattg
     1081 atagatagat attagcgcag ctaaaaaaat tatgataatg aaaacattat taaaatacac
     1141 catttttttc tttttttcct cttcactttg tgaaaagtct gcaggaaaa
//
LOCUS       DAZULO010000215         1182 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000215 DAZULO010000000
VERSION     DAZULO010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.224"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..102
                     /locus_tag="ACG8OA_002926"
     CDS             <1..102
                     /locus_tag="ACG8OA_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HFS4977646.1"
                     /translation="LNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            complement(350..898)
                     /gene="aac(6')"
                     /locus_tag="ACG8OA_002927"
     CDS             complement(350..898)
                     /gene="aac(6')"
                     /locus_tag="ACG8OA_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS4977647.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          369 a          236 c          170 g          407 t
ORIGIN      
        1 ttaaataatt ttcgttatat tgtctattat tgccatacaa agataaaaaa gaataaacaa
       61 aaagaaactg tgacaaaagt cttgtcacag tcccttatct gaataaacgg tgttcaaaag
      121 ccagtttctc ggtattaagc caaatttatg caaaaatgta gaaagccatt ttcacaaaat
      181 ttttcttact actcgaaact aagcgacttt ttcacaacct ctggtacacg gtgttccaaa
      241 agtagcttct tcggaaataa gttggaattt cacaaaaatt caagaagtaa ttttcgtaaa
      301 ttccttctta tttcttgaag ctgattactt ctgtcacaac ctctttttat tattgagaat
      361 ttggtcgagg aataatcgtt tttgccatcc aaatatccgg tttgtcccag ccatttgcat
      421 ttggtaagac acctacgatt ttataaccta atttttcata gaattcatac ggatgttcac
      481 gaaggttctg gatggaagcc actttatcaa atgtatgctc atacaggtcc gtttgactta
      541 acgttgttcc atggtctaaa tcatccgtac ctaaataaat cgtgattcct cctctggaag
      601 ctacttcttt ttctaagtaa ttgactaatc gagtacctat ttggttcttt cgtcgggagc
      661 tttctacaac taatggatgc aattcccaac ctgtgatacc gtattgaggg attgcaccaa
      721 taaatcctac taactcatct tggtctaccg ctgctaccgc gattcgttct ggattcatca
      781 tttcttctac ttcttctgcc gagctgtctc catattcttc cggccaagtc agtctcagta
      841 aatcagaaag ctgatctttc aatactggat tattacggtc aaattcactg attatcatac
      901 gtcttccccc ttgttcttgc ttattctaac agtccttttg aaaaaacgaa atgttctcgc
      961 tcaaaaatct ttagctacca atcagtatct ctattctttc attatttatc ttacttataa
     1021 aataaaaaaa ttatacagaa acactttatt caaaatagga aggatcagga aagccatcaa
     1081 ttggaggaaa aaactctgtt tgttcatcat gatatagatt atcttttgct ttaaaaaaaa
     1141 ccaattatga attgacaaat aaaacctgtt ttcatataat aa
//
LOCUS       DAZULO010000216         1157 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000216 DAZULO010000000
VERSION     DAZULO010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.225"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1157
                     /locus_tag="ACG8OA_002928"
     CDS             <1..>1157
                     /locus_tag="ACG8OA_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFS4977648.1"
                     /translation="QKKKTLIVDAVFSPAPHTCRNCGSTVVDGNGKVIVVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNAKVV
                     IDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKT
                     WKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFSLLAELP
                     ETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYGF"
BASE COUNT          379 a          229 c          226 g          323 t
ORIGIN      
        1 ttcagaagaa aaagacgttg atcgtcgatg ctgttttctc acctgctccc catacctgta
       61 gaaattgtgg ttctactgtg gtagatggaa acgggaaagt aattgtagtg aaaaatggga
      121 agaaggaaac gattgtccgc tttgaacaat acaatcacat gcctttggtt atgcgcttaa
      181 aaaagcagcg ctatacctgt aaaaactgtc gaacccattg gacggctcaa agttattttg
      241 ttcaacccag acattcaatc gcgaatcatg tcagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa attgtcaggt atctttgacc accgttattc
      361 gtacgttgaa agagtttaaa agctacttac caaagcaatc taagaggatt ctccccagag
      421 tattgatggt tgatgaattt cgttcgcacg cttccataga agataaaatg agctttattt
      481 gcgcagatgg cgaaacagga caattaattg atgtcttgcc tacgcgtaaa ttgcctcgat
      541 taacaagcta tttcttaggc tgcgctaatc ctgaagaagt aaaattttta gtcacagaca
      601 tgaacgccgc ctacttccag cttaccaaac gtattctgcc aaatgcgaaa gtagtgattg
      661 atcggtttca tattatcaaa cacatgaatc aagcctttaa tgagttgcgt atccgtgaaa
      721 tgaatgaact tcgtaaagta ggacagaaaa gtcaggcaga aaaactgaaa aagaactggc
      781 gctttttgct aaaaaatcgt gcaaacatca accattatga atacaaaaca tggaaaagtt
      841 ttcgagcacc aaaataccca tttcttacgg aagcgatgat gattgatcga ttgcttggct
      901 tttctacgtc cctgaaggag gcgtatccct attttcatga attagttgaa gcctttcgag
      961 acaaagaccc tgacttattt ttctccttat tggcagaact tcccgaaacg ttagatgacg
     1021 gctttcggga aaagctccaa aaccttctga cctatgaaga aggcatcacc aacgcgatga
     1081 tctatcctta caccaatgga aaaatagaag cgaagaatac ccatataaag acaatgaaac
     1141 gagtatccta cggattt
//
LOCUS       DAZULO010000217         1144 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000217 DAZULO010000000
VERSION     DAZULO010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.226"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..924)
                     /gene="xerC"
                     /locus_tag="ACG8OA_002929"
     CDS             complement(19..924)
                     /gene="xerC"
                     /locus_tag="ACG8OA_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFS4977649.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          380 t
ORIGIN      
        1 ttgttctttt ccttattttt acgctcttgg atggaaagaa cgatagtttt tttgtaaact
       61 ttcttttgtt acatgggcat agatctgggt agtcgataaa ttggcatgtc ctagcaattc
      121 ttggactgtc cgcaaatctg ccccattatt taataaatga gtagcaaatg tatggcgcag
      181 catgtgagga tggatatcac tatccaaact gctttttttg atcatttgat tcaagacata
      241 ttcaattcca gtaggcgtga ttggttttcc atgatgattc acaaaaacga tatcatgctc
      301 ttcgtgatat tttgtcatca atacctggcg gccttccctg aaatattctt gcagagcatc
      361 ttgagcaaaa gaacctagag gagcatatct ctctttgttc cctttaccat ggatcaacat
      421 gacctctgaa tcaaaatcaa tgtctgatac cgacaaactg ctacactcac ttaaacggat
      481 gcctgacccg taaaggactt ccagtaaagc gcggttcctc aaatccaaag gactatctcc
      541 tgctacgctg tcaaaaagtg cttgcatttc attttcataa aaaaaacgtg gcagtctcaa
      601 atttttcttt ttgagatgaa cgtaagaaaa tggattttct tttatcactt cttctttgag
      661 aagatactga taaaacgaac gtaggctggc tatcttgcga ctgatagaat ttctgctata
      721 gctaggacga tgatcagcta atgcactcaa atagacacga accgtaaaat attccgcctt
      781 cagcagatca gcgtctccag attcctccaa aaaatgtttg aaatggagaa tgtcttcttc
      841 atatgcttct ttcgtttttt ctgaatatcc tctttcgaca atcaaataac gcaaaaattt
      901 ttgttcccac gtggcttctg tcattttctc accttccttt tgtcccagag aataatttag
      961 cacagcgttt ataaaaagac aaataaattg tcagaattta accaatattt ctaagtttgt
     1021 tcacaattgg cgcaattcct cacattttgt ttgagaaatt cttaagaaaa acgcaagaaa
     1081 aagggctcta taatttatgg gactgatgaa tcagaattga ttcttcagcc ccattttcat
     1141 ttta
//
LOCUS       DAZULO010000218         1137 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000218 DAZULO010000000
VERSION     DAZULO010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.227"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..355
                     /locus_tag="ACG8OA_002930"
     CDS             <1..355
                     /locus_tag="ACG8OA_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977650.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="ACG8OA_002931"
                     /pseudo
     CDS             615..910
                     /locus_tag="ACG8OA_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            985..>1137
                     /locus_tag="ACG8OA_002932"
     CDS             985..>1137
                     /locus_tag="ACG8OA_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFS4977651.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT          431 a          135 c          237 g          334 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa gggttctgtt gcaaagtttt ccaaaaaatc tattttagtg taaaattgag
      961 aaaaaagaca gagaggacag agtaatgaat cattttaaag gcaaacaatt caaaaaagac
     1021 gtcattattg tcgctgttgg ttactacctg cgttacaatc taagctatcg tgaagttcag
     1081 gaattgttat atgatcgtgg aataaatgtt tgtcatacta cgatttatcg ttgggta
//
LOCUS       DAZULO010000219         1136 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000219 DAZULO010000000
VERSION     DAZULO010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.228"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..425
                     /locus_tag="ACG8OA_002933"
     CDS             15..425
                     /locus_tag="ACG8OA_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4977652.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            439..795
                     /locus_tag="ACG8OA_002934"
     CDS             439..795
                     /locus_tag="ACG8OA_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4977653.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          398 a          170 c          217 g          351 t
ORIGIN      
        1 aaatgagtga gaaaatgata aaagtatata cttcgcctag ctgtacttcc tgtagaaaag
       61 caagagcatg gttgcaagcg aatcaacttg aatttgaaga gaagaatatt tttgccgaac
      121 cgttaacaga aagtgagatc aaaaagatct tggcagcgac tgaaggagga gttcaagata
      181 ttatttccac tcggtcgaaa atttacgaaa agctgggcat agattttaac gaattaactt
      241 taaagcaaat gatcgtctta ttcaaagaat atccttcatt attgagacga cctattttga
      301 ttgatgaaaa aagaatacta atcggattta atgaagatga gatccggtca tttgtccctc
      361 gtgaaattcg atgtgttgcc agaaatctga tctatttaca aaacaataac atcttaaatt
      421 catagggagg gagccttcgt ggaagtgaaa gtctatggat cagcgcactg tgtaacaagt
      481 ctgaaggcga aacaatggtt agaacaccat caattgcctt atcaatttgt tgacttagaa
      541 gaacgcgatc taagtacaga agaagcagaa gaattatgca gttttgaaga agtgcaagca
      601 gaacagctat ttgcgacttg gtctgaggct tttcacaaca ttgcgattga cctttcctgt
      661 gtaggaaaaa aacaactgat ttttttatgc acacaacata ccaatctcct cagacgtcct
      721 ttgattatta tcgatgaagt tctatttatt ggatacaatg agcagttgat ggaacagctt
      781 atcttaaata aatagacttt aactgtttta atcaagtcac tgagcatttc atacagttgt
      841 aacagtcgaa cacttagcat caagcgtgtt atgtgttctt tttttgactt tatttttcta
      901 ctatagagac atgctcaatt tatgtggtca aaattcaaat ctaggtgggg atatagagtt
      961 aaaaatatat tgacatgtgt aaatttacat gatatattca tttctgttgt tttaagtagc
     1021 aaagcaataa aatagtattt taaaaaacat ataaaatagt tgttgacatg ttagaaatga
     1081 catgataaga taactgagtt gttaaaaaag aaccacttgc gaagcttgat tctatc
//
LOCUS       DAZULO010000220         1125 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000220 DAZULO010000000
VERSION     DAZULO010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.229"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..>1125
                     /locus_tag="ACG8OA_002935"
     CDS             14..>1125
                     /locus_tag="ACG8OA_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977654.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRR"
BASE COUNT          363 a          152 c          153 g          457 t
ORIGIN      
        1 aatataaaaa aacatgaggt ggtatatgaa tttgaaaaaa attttttctg taaaaatatt
       61 tatagtttat gcatttgtta gtttattata tttatttcca gcatttaaaa acatggaaat
      121 atttggcttt tatgatacgg catttcacat taatcgtgcg ttaagtttag aatctatttt
      181 tagctcccct attaattttg aaacgtttag atcatatggc atgcaagtaa ataattttta
      241 cccatggtta actctatatc ctttattttt attgatcaag tttactaatt tagcaatagg
      301 ctataacctt tttctttata ttgttacgct cattactcta tttatatgtc attatgtaat
      361 gtatgaaatt accaaaaaac atgttactag ctcctttttc gctattatat atacaacatc
      421 ttcttttagg agtgtagaaa tatttcttcg aggggcaatg ggggaattac tcgcaatgtc
      481 aatacttcct cttattttac tcgggtttat aaagctttac gactcaaaaa aagaatcttg
      541 ggttatgttg gcgattagca tgactttatt aatttacact catgttcttt ctgtagctat
      601 gactatggta atgattataa ttgctttaat tattcaattt tatcggaaaa agaaaataga
      661 aatcttctta attattaaat taataaaagc tgctatagtt actttacttc tttcactagc
      721 gtttataggt cctatgattg agcagacatt gtatcaagat ctgaatagac cttatgtgga
      781 tatattacaa aaaagagcaa tttatctggg taaagagttt ttgattggaa gtatagattc
      841 agaattacta tcatttggta ttggcttaac tttgcttatt tgtttaatta tactaacatt
      901 taactttaaa aaattaggaa tcttatctaa aatcacttat ataatgggta taatatctat
      961 tctactttgt acaaaaatat ttccatggtt tgccttacaa aatactcctt taaatattat
     1021 tcaagctcct tggcgtttta tgatattttc aacattgttc atatcttttt cagtatctct
     1081 atttatttct ccacagttgg aaaaattttc ttatacaagg agaag
//
LOCUS       DAZULO010000221         1106 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000221 DAZULO010000000
VERSION     DAZULO010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.230"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(627..713)
                     /locus_tag="ACG8OA_002936"
                     /pseudo
     CDS             complement(627..713)
                     /locus_tag="ACG8OA_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          401 a          188 c          150 g          367 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgagt gacttctgcc taattacgtt
      121 aaaaaatatt gaaagtaact ctatgtagca aaaaatatct agaatttcat ttcctcaaaa
      181 ctgatcgata ttgtatctaa aaatatatac tacctaaaat aggtagtata tattaaaata
      241 tgtgcttttg ttagaaaaat caatacataa attatcatat ctgtatatgt catttaggta
      301 tttcagttat gttataatag ttttgctgtc tgctaccccg gcctaatctg gcaaggggag
      361 gaggagatat aatgaaatac ctttttgatt ttgtttttgc aatcaagcca cttgcagatg
      421 tagtgtgcag ttacctctgc aaatggcttg attgcaaaat tgccaaaaaa gacagtaagc
      481 ctacccgtta attctataat atagaaacgt aaaaaaaacc ccatcagcct gcacgctgat
      541 ggggtttggt gatataatga aatacctctt tgttactcat aattatacca taattagtca
      601 cgaatgcaac caatagaaaa aactacttag aacggtagat catcgctaat aacaatgcat
      661 tgtccaccaa atggattttg tgaagcatta aatacattgt tattaattgt cgctgctttt
      721 aactaggcct ctccccttct attataaaag aagatatata tcatttctta attacctact
      781 accttaaacc aaaatttaag tactacctaa tttaagtagt acttaaacaa tctaaaaaat
      841 agttatccca attgttatgc ttttataata cgattatttg taacaaaatg aatattatat
      901 aattaaacac taccatattt aggtagtgtt taattattag caaaacaaaa aatatttatt
      961 ctattctctt aacttcaact aagttatgct gcacaccttt atataaatca tatgcaactc
     1021 attagctaga caaactacaa gcatgtagtt ttatctgtta attatgtact acctaaataa
     1081 agtagtttat aattagcaag tatata
//
LOCUS       DAZULO010000222         1105 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000222 DAZULO010000000
VERSION     DAZULO010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.231"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACG8OA_002937"
     CDS             <1..1087
                     /locus_tag="ACG8OA_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4977655.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          402 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaa
//
LOCUS       DAZULO010000223         1103 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000223 DAZULO010000000
VERSION     DAZULO010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.232"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..634)
                     /locus_tag="ACG8OA_002938"
                     /pseudo
     CDS             complement(1..634)
                     /locus_tag="ACG8OA_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          341 a          190 c          189 g          383 t
ORIGIN      
        1 aggtaaaaaa taagtctgat tccttgtctc taaaggcttc ggtcagctca tggaaaaaag
       61 tataggcgtc ctttaaaggc gttgaaaatg ccagcaatcg atcaatcatc attgcttcag
      121 tgagtagcgg gtatttaggt gcacgaaaac tgcgccatgt tttgtaatca tagtgattga
      181 ttttttgacg gttttttaga agaaagcgcc agttcttttt cactttctcg gcttcccgtc
      241 gttgccctaa ctggtgtaat cttttcattt cttgtacacg aaaatcattg aacgcctgat
      301 tcaaatgttt gatgatatga aaacaatcaa tgacgacttt cgcatttggt aagacacgtt
      361 ttgtcagttg gaagtaggcg gcgttcatat ctgtcactaa atattccacc gcttcaggat
      421 ttgcacagcg actaaaatag tgtgttaatc gagacaattt tcgtgtaggc aaaatatcaa
      481 tgagttttcc agtctcgcca tccgcacaaa taaagctcat tttatccaca attgaagcgt
      541 gcgaacgaaa ttcatccacc atcaatactt ttggcagggt cctttttgac ggcttcggaa
      601 gatagttttt caactctttc aatgtacgaa tcatatacct aaaataacaa tgggactgaa
      661 gaatcaattt tgattcatca gtcccataaa ttatagagcc tatattttgc gaattgcgct
      721 acggtttctt cttttctttt gtctgtaata tgaatatata gatccataat gatttgaata
      781 gaagagtggc ctaaacgctc ttgcacatct ttaatatttg cactagcttg ctaaaagcaa
      841 gctagcatgt gtgtgtctaa gaccatgaat agtaattcgt ttaagattgt ttttcctgat
      901 gattacatct aaccattttc gagatttaga taattgaagg tgttcatttc tttctggtaa
      961 aaatttaaat atataactac attttctcta ctaactttga aacactactt ttgtaatata
     1021 aaaaatattt tattaataat aaaattaatc acattttata tagtgagtaa tctagtgttt
     1081 taaaaaaata aatgatacta tat
//
LOCUS       DAZULO010000224         1069 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000224 DAZULO010000000
VERSION     DAZULO010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.233"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACG8OA_002939"
     CDS             <1..969
                     /locus_tag="ACG8OA_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFS4977656.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          185 c          214 g          302 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccat
//
LOCUS       DAZULO010000225         1060 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000225 DAZULO010000000
VERSION     DAZULO010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.234"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..994)
                     /locus_tag="ACG8OA_002940"
     CDS             complement(38..994)
                     /locus_tag="ACG8OA_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977657.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          380 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttttctg
      961 tttttataat tataggtagc gtatacaatt ccacaaaact tatctctgtt gagttaaaaa
     1021 gattatataa aaaaagccga atgtttttag gattccctgt
//
LOCUS       DAZULO010000226         1051 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000226 DAZULO010000000
VERSION     DAZULO010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.236"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ACG8OA_002941"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ACG8OA_002941"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFS4977658.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DAZULO010000227         1039 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000227 DAZULO010000000
VERSION     DAZULO010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.237"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..725
                     /locus_tag="ACG8OA_002942"
     CDS             33..725
                     /locus_tag="ACG8OA_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS4977659.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
BASE COUNT          457 a          118 c          156 g          308 t
ORIGIN      
        1 tttaaggaaa ttttttgaag gagaagataa aagtgagaaa aaagattatt attaatgctg
       61 gagtaattat aggattgatg ttagtaattt tctccggaaa gcaatatgct gataatattt
      121 tattatgggg aggggaagat aatattgaaa agatcaacaa caatttaact atattagaca
      181 cagctctaga aaataaagag caaaaaatta gtattttaaa ttcacaattg tcttcaaaca
      241 accaaactct tgaacaacta agaactaata ttgaagtttc caaacaaaaa gtggcatctt
      301 tagaaaacga caagaatcaa ttagtatcag agaaaactaa tttagaaaat caattgtcag
      361 caaaagactc tgaattgcaa ggaaaacaaa atgaaattaa tgcgaaaata gacgaaatta
      421 accaaaaagt agctgaaata aatcgattaa atagcgattg gaataatcgt ttacaaaatg
      481 aaaagaacaa atttaatgaa gaacaatcta ctattaatag tttaaaccag caaattaatc
      541 aaatgttgag tgaaaaaaat aacattcaaa atcaactgca agctactcag caaaagctag
      601 aagaagaaaa gtcaaaaaat agtagtttga ataattacat tgaaaagctt caaaaagcta
      661 aaaatgatgt agaggatacc gcaaataaag cacaacaaat tgtaaatgaa catacaatcc
      721 aatagatata gttttaattt aacaaatggt atgtttggag ataatgagat agaatataaa
      781 tgtcataata ttatacaatc taaacaccgt ttccttttct atttttgatg acatcagtat
      841 atagctactc gaaagagtag tctttttatt attctcgctt attattgata tgaaaatttc
      901 gatttgacaa aaggtaccga ataattttgt aatatgaatt attatcaaga agtaattatg
      961 atgataacat atggaaacta gttagtaata ataacaggtg attaaacttt tgaacgactt
     1021 gtaattctat attttttct
//
LOCUS       DAZULO010000228         1038 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000228 DAZULO010000000
VERSION     DAZULO010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.238"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(155..>1038)
                     /locus_tag="ACG8OA_002943"
     CDS             complement(155..>1038)
                     /locus_tag="ACG8OA_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFS4977660.1"
                     /translation="RERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIE
                     YTENLSKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGI
                     IPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVL
                     KVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLR
                     ESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGV
                     SPKQYQLGLVDNFNNIS"
BASE COUNT          359 a          179 c          122 g          378 t
ORIGIN      
        1 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
       61 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
      121 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
      181 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
      241 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
      301 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
      361 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
      421 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
      481 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
      541 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
      601 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
      661 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
      721 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
      781 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
      841 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
      901 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
      961 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     1021 tatagagtcg ttctctat
//
LOCUS       DAZULO010000229         1030 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000229 DAZULO010000000
VERSION     DAZULO010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.240"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            60..548
                     /locus_tag="ACG8OA_002944"
     CDS             60..548
                     /locus_tag="ACG8OA_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS4977661.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
     gene            535..>1030
                     /locus_tag="ACG8OA_002945"
     CDS             535..>1030
                     /locus_tag="ACG8OA_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977662.1"
                     /translation="MEKIKQTFPLINKYWNKKKIFIFFITFLLSSLFSLIFPYLFSNV
                     IDLYLENELSQNIVRIVTILIFQLFLVLIKEVYGYIKTIYAFEVDLSMKIDFLKVLQK
                     KNLFFSQTKHSGEIQFRMFSDISTIVNTIFIFSVELPVIGVLVLVAVFIISKVNLIFL
                     IFSLI"
BASE COUNT          381 a           92 c          171 g          386 t
ORIGIN      
        1 gtttttatgt gaaaacatga gcaatagaat tgtactagta ggataaggat ggatggaaaa
       61 tgaaaaaaac tatttttgtt ttagcgattt ttatatgttt tgggctactt ttggtaggct
      121 acttgttgtt taattctaat gaaatgagaa ctatttctac gaatgacttg ttaaaaaaaa
      181 gtgaattaaa ggataaaaaa aaagaatctc ttgcctttat aatgtttact caagaagagt
      241 gtgagtattg tatagcaata aatccaatca tagatagtgt aaataaggaa actttgaata
      301 agataaagaa atatgatata tctgtagatt ttcaatctaa acaaaagtgt aaaattaagt
      361 ttgttccgac tattgttgca tatagtaaga ataaagaagt ggggagaatt gacaatgaat
      421 tagataattt gtatcgaaaa aagcaagatg gttcaattgt aatagacaag aaaaaaacaa
      481 agacattgct tatagattgg atgaaaaaaa tggataaaca aggagaatat ttgtatggag
      541 aagattaaac agacatttcc gttaattaat aaatattgga ataagaaaaa aatttttata
      601 ttttttatta cgtttctgct gtcatcttta ttttctctaa ttttcccgta tctatttagt
      661 aatgttattg atttatattt agagaatgag ttatcacaga atattgtgcg aatagtaacg
      721 attcttatat ttcagttatt tttagtctta attaaagaag tgtatggata tataaaaact
      781 atttatgcat ttgaagttga tttatcaatg aaaattgatt ttcttaaggt tttacaaaaa
      841 aaaaatcttt ttttttcgca aacgaagcat tcaggagaaa ttcaatttag gatgttttcg
      901 gatatttcta ctatcgtaaa tactatcttt attttttctg ttgaattacc tgtgataggc
      961 gttttagttc ttgtagcggt atttattata agtaaagtga atcttatttt tctgattttt
     1021 tcactaataa
//
LOCUS       DAZULO010000230         1017 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000230 DAZULO010000000
VERSION     DAZULO010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.241"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..885
                     /locus_tag="ACG8OA_002946"
     CDS             <1..885
                     /locus_tag="ACG8OA_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFS4977663.1"
                     /translation="NIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWLAKKNGIRQR
                     IAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQNHPFEIVHNG
                     IDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDS
                     YLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMV
                     GLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQELKS
                     YDVSYTTEQMESIYSRDH"
     gene            918..>1017
                     /locus_tag="ACG8OA_002947"
     CDS             918..>1017
                     /locus_tag="ACG8OA_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HFS4977664.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHI"
BASE COUNT          394 a          112 c          190 g          321 t
ORIGIN      
        1 aatattttta aatatttaat agaagtgaat aagttactaa aaaaagaatc tttttctata
       61 gttcacaatc aggtttattt tggcggggga attaatttat ggttagccaa aaagaatgga
      121 atcagacaga gaattgctca tagccatgca actgaagatg gaaaatctca aaatattgta
      181 atgaatgtat tgcgtaaatt tttaaccaaa ttgttgttac aaaatgcaac agactacctt
      241 gccgttagtc aagaagctgg tgaaagtctg tttcaaaacc atccctttga gatagttcat
      301 aatggtattg atttagagct ttatagtaaa aattcagaag ctaaggttaa taagagaaaa
      361 gaattggata tctcaatgag tacttttgtt gtgggaaaca ttggacgatt agagaaacaa
      421 aaaaatcaat cttatttgtt agagattttt ttagaaatac taaaaatgaa ttctgattct
      481 tacttgattt tagtaggtga agggtcactt agaaataaac tgaaagccaa agcagtagaa
      541 ttggatatag agaaaagagt aaaattttta ggagaaagaa acgatattcc cgagttatta
      601 tctgtgatgg atgtttttgt aatgccttct ctttatgaag gtctgccaat ggttggatta
      661 gaagcacaag cagcaaaatt aaaattagtc ttagcagata ctatttccaa agatacaaag
      721 ttaacaaata atgtggattt tgtatcatta gatgaaacac cacaagaatg ggcaaaaaaa
      781 gtaataaaag agccgtatgc taataaaata acacaagaac taaagagtta tgatgtttcg
      841 tacactactg aacaaatgga gagtatatat agtagagatc attaaaaatg ttcaagatat
      901 tttaggagtg attatttttg aaaatatgtt atttgatatt ggcatacaat gatattggca
      961 acttaaagag attggtcaat cgattgaacg aaaatgcagt ttttattatt catatag
//
LOCUS       DAZULO010000231          997 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000231 DAZULO010000000
VERSION     DAZULO010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.242"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..913)
                     /locus_tag="ACG8OA_002948"
     CDS             complement(26..913)
                     /locus_tag="ACG8OA_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4977665.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          323 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaatt
//
LOCUS       DAZULO010000232          994 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000232 DAZULO010000000
VERSION     DAZULO010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.243"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>994)
                     /locus_tag="ACG8OA_002949"
     rRNA            complement(<1..>994)
                     /locus_tag="ACG8OA_002949"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DAZULO010000233          975 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000233 DAZULO010000000
VERSION     DAZULO010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.244"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>975
                     /locus_tag="ACG8OA_002950"
     CDS             <1..>975
                     /locus_tag="ACG8OA_002950"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4977666.1"
                     /translation="QLPLKKTIENTKSVNQSITGELNAHFDQLEIISAYNFENISILK
                     FDSRFKELFNVFKKQNKLLTLGSCLAQVIFQLWIGGIFLIDVILLAKGTISITNFVIV
                     FMYANMVPTLWTTLISIIWKWQDYSVSLNRYYEYYSNNNKVAGTKKINKINSITGQSL
                     NFSYNKRKVLSNISFSTEMNKLFMISGENGTGKTTLMKLFTKQLPVSNNQLFVNGIDI
                     NMIDNESLRRLISIATQIPYIINSSVKENILMGTKNYDKKKFSKLIELLGISKFMTKL
                     GITLDDNIYDEKILLSGGEKQKISLARALIKSASVIILDEPFRNLDNET"
BASE COUNT          406 a          107 c          147 g          315 t
ORIGIN      
        1 tcaattaccg cttaagaaaa caatcgagaa tacaaaaagt gttaatcaaa gtattacagg
       61 agaattgaat gcacattttg atcaattaga aattataagc gcatataatt ttgagaatat
      121 ctctatttta aaattcgatt ctagatttaa agaattattt aatgttttca aaaaacaaaa
      181 taaattacta acactgggaa gctgtttggc acaggttatt ttccaattat ggattggagg
      241 aatatttctt attgatgtta ttttattagc taaaggaaca ataagtatta ctaattttgt
      301 aatagtattt atgtacgcta acatggtccc tactttatgg actactttaa ttagtattat
      361 ttggaaatgg caagactatt cagtgtcatt aaatagatat tacgaatatt attcaaataa
      421 caacaaagtg gctggcacaa aaaaaataaa taaaataaat tctattactg gtcaatcatt
      481 aaatttttca tataacaaaa gaaaggtatt aagcaatata agtttttcaa ctgaaatgaa
      541 taaactattc atgatatcag gagaaaatgg aacagggaaa acaacactga tgaaattatt
      601 tactaaacag ttgccggtta gtaataatca attattcgtc aacgggatag atattaatat
      661 gattgataac gaatcattaa gaaggttaat ttcaatcgca acacaaatac catatataat
      721 taatagctcc gtgaaggaga atattttaat ggggactaaa aactatgata aaaaaaaatt
      781 cagtaaatta atagaactat tgggaataag taaatttatg acgaaattag gtattacttt
      841 ggacgacaat atttatgatg aaaaaatatt attatctgga ggagaaaaac aaaaaataag
      901 tttagctagg gcgcttatta aatcagcttc agttattata ttagatgaac catttagaaa
      961 tttagataat gaaac
//
LOCUS       DAZULO010000234          964 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000234 DAZULO010000000
VERSION     DAZULO010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.245"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..>964
                     /locus_tag="ACG8OA_002951"
     CDS             111..>964
                     /locus_tag="ACG8OA_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977667.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGT"
BASE COUNT          384 a           95 c          173 g          312 t
ORIGIN      
        1 ttttaagaat aaagtgtgaa ttgacgtatc ttttattctt ttttctagtc taccggtaaa
       61 ttttgagagg agcgtcatat gaaatttatt atgaataagg agatgtatta atggctacaa
      121 attatgcaaa aaagataaat tttgatgtgg ataaacttta taaaagtttg atagttgttc
      181 ccaaaaataa atcagaagat gggaattatg agttagaagt tttgcaattt aagattgaac
      241 aaaagtccag tgaaagattt atgagcaatc aattagtgaa aatcaaaaaa tggctcccta
      301 tgattgatca attagtatct aataataagg aagctaaaaa tttatgtatt tttgtcttat
      361 tattacatgg aagagttatt aacttagtta atcgaaaaag tacgactaaa tttgcaaact
      421 cattctggtt tttaaaaaat attaataaaa cagtggaaaa gaatggagtt tataaaggtg
      481 attttcattt tcaaaatgct gagactattg aacgagaaca tcattatttc gatttattag
      541 tgaatacagt ggtagaatta gcgaaatggg caaagtcaaa cgaaacttat agtcaagtac
      601 ttaaaagaat tatgagtcaa gaaacttcat ttatctatga gttaagcaag tcaaatttga
      661 aatatactta taatgaagat gaacttgaaa ttttagataa tccagaaaat tcacaatgga
      721 ttaaagagtt gaagagagaa gactttattg agacactaag aattctaaaa tcgttatttg
      781 atttgaaatt agccaaagct accatcatgg gaattgaaag agattcatat tattgggagg
      841 aaattggact atatgctccc cagattgaag gtgaagataa agagaaaagg atagacaaca
      901 ttattaacta tattaatagt ttaagtgaac ctttaaaatg tagaggactt gattttatag
      961 gaac
//
LOCUS       DAZULO010000235          959 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000235 DAZULO010000000
VERSION     DAZULO010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.246"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..208)
                     /locus_tag="ACG8OA_002952"
     CDS             complement(<1..208)
                     /locus_tag="ACG8OA_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4977668.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEG"
     gene            284..356
                     /locus_tag="ACG8OA_002953"
     tRNA            284..356
                     /locus_tag="ACG8OA_002953"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:317..319,aa:Lys,seq:ctt)
     gene            518..739
                     /locus_tag="ACG8OA_002954"
     CDS             518..739
                     /locus_tag="ACG8OA_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977669.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
BASE COUNT          324 a          158 c          135 g          342 t
ORIGIN      
        1 taccttcatc ttgtattttc aatatatact caatgacagc ttttttaggt attttgtacc
       61 ttgctccaat ttttatattt tctattgatc tatctttcag caaattataa gctttatttt
      121 ttccaatatt taacatttta gttaaatcag taattttcaa tatcgatgga taactttcaa
      181 atattttttc ataatcttga aaatccataa aattcactct caattctgat aataagtatt
      241 gcactttttt attttaggtg ttagaataac aaagtattaa tttgggctgt tagctcagtt
      301 ggtagagcag atgactctta atcatcgggt cgcgggttcg agcccctcac agcccattgg
      361 gtgccaaacc cttaaataca gtgtaaacgt tggtgcttag catttagaaa acgtatatac
      421 tgtatttttt tgttttcaaa agagactgta gtaccccttt ttttcactaa aagcccactg
      481 tagtcattga tatttttgat aagaaaggaa cataattatg cgtaatgaaa ttgctaatat
      541 aacaataatc cgagaaactt tacaaaacca tactgcaaat gagatctcta aacatacagg
      601 attaaatttg agtactataa aaaaactaaa aagtggagaa cgttcaatag aaaaactaaa
      661 tttacatgat gctatttgcc tcactgaatt tggtcttaaa aataatagaa aaaatgttga
      721 aatcaacata tggaaataat tcttaatcag ctgcttttgt caaaagcaca taggaatttc
      781 acctctctcc aagtttacgg cgagccttac ggaagtatca ttatcccaac ttcaaatcat
      841 cgctttttat tgactataaa aattatttta gaatgtttat cgctccttct ttacagtaag
      901 aaatcatggc gcaactaaaa tatgctcctt gaagtgttaa cgtactgata gaatgtatt
//
LOCUS       DAZULO010000236          931 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000236 DAZULO010000000
VERSION     DAZULO010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.247"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>931
                     /locus_tag="ACG8OA_002955"
     CDS             <1..>931
                     /locus_tag="ACG8OA_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4977670.1"
                     /translation="HCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFY
                     RPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFS
                     RFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETT
                     VRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAA
                     CPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNA
                     LHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          286 a          190 c          180 g          275 t
ORIGIN      
        1 acactgtttc attagtcaaa aagttcgttg gtctgtcatt gctcgcttaa aagagaatac
       61 ttccatgacg gaaatcgctc gtcagaaaaa tatttccact tcttcggtct atcgtgtcat
      121 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
      181 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      241 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      301 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      361 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      421 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      481 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      541 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      601 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      661 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      721 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      781 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      841 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      901 gtacggcttt cgcaactttt acaatttcaa a
//
LOCUS       DAZULO010000237          928 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000237 DAZULO010000000
VERSION     DAZULO010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.248"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..538
                     /locus_tag="ACG8OA_002956"
     CDS             <1..538
                     /locus_tag="ACG8OA_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977671.1"
                     /translation="KIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDET
                     DIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISP
                     ISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKI
                     LLPENWAELRKLVEYGII"
     gene            525..>928
                     /locus_tag="ACG8OA_002957"
     CDS             525..>928
                     /locus_tag="ACG8OA_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS4977672.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFV"
BASE COUNT          318 a          122 c          138 g          350 t
ORIGIN      
        1 caaaatagat gaaaacagta aattgatgaa aaacatacaa tcaatctttg ggatagaaga
       61 agtttttgtc ggagataaat gtatcttttt tgatcaacct ttattatctg atggttatgg
      121 catagatgaa acagatattt tcgctaaagt aaatagtata ttttctggaa aaaaagaaac
      181 tttaattaag cttcatccta gagcaaggac aaatcgttat ggtgaaaaaa acatattgaa
      241 ttccagttta ccgtttgaag tcttgctact tgaaaatgac ttctcttcaa cacttttgat
      301 tagtcctata tctacaattt ctttttcacc atctatgatg tttgaaaaga cgatcaactc
      361 tattatgttg gctaaattaa ttttaaatga atatacagca ttatctgaag ataaaaaaga
      421 aacattgaca aatattgttc ttttctgtga acattataac cgtctatcag aagaaaaaat
      481 tttactccca gaaaactggg cagaattaag aaagttggta gagtatggga ttatttaaca
      541 cactttccaa gtcaaaatta gttaagtcca gtttctggta tactataggg aattttttta
      601 ttaagggaat ttcattcata actattccta tttttgtaaa tattactact caagcagaat
      661 atggtttgat taataatttc acttcatttg tatcaatttt ttcgattctt gttgggctaa
      721 gcctaaattc ggcaattaat aatgcaagtt ttgactataa agatgacata aatgaattta
      781 tgtcatcggt actttctcta gctacacttt cgttaattgt gttcttatta atagggaaca
      841 tatatttttt gttttcaaat gctttttttg atatgaacca acttgtattt aatttgatgt
      901 tattacaaag ttttgctagt tttgtaat
//
LOCUS       DAZULO010000238          921 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000238 DAZULO010000000
VERSION     DAZULO010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.249"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(145..>921)
                     /gene="menA"
                     /locus_tag="ACG8OA_002958"
     CDS             complement(145..>921)
                     /gene="menA"
                     /locus_tag="ACG8OA_002958"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFS4977673.1"
                     /translation="NYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVI
                     GVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYV
                     NTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDI
                     ENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHL
                     DQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
BASE COUNT          327 a          191 c          159 g          244 t
ORIGIN      
        1 tttcacaacc tcctgataaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc
       61 aaacgaaaaa tataaggagc tatttttatt tggctgttct tattacttga tgcagggcag
      121 ttttttcaca acctcttcat gctgttaaat ggcttgcata atgattgtta gtaatagccc
      181 caatacataa ctggcattga ataagaccat atttttgatg gaatagctga aactgatagg
      241 ctggggtaac gtttgcttga attgatccaa atgcttcttc acaattggca aggtcaagaa
      301 ggtgaccaag atcggccatt catagatacc gctcaacaac cccattaaaa tcacgaaata
      361 gcaagcatac atcaatagct ggaataagac tgttcccgca ggacgtccga tataaaaaac
      421 tagtgtatag cgatgatttt caatatcacg gtcaagatcc cgcaaattat tagccaacat
      481 gatattggca atcgtaaaca ccaagggtaa ggaagctaat acgattgctg ctatagccca
      541 aagatttcct accaaatcaa attgtccaga tgaaaagttg actgtcagat catagacatc
      601 tgaaccgaat gtattgacat aaacgaccat ggcaaaaatt ccaaggccca tagtcacccc
      661 actgaaaatt tcccctaaag gcattcgaga taaaggaatc ggtccgaatg tatagaatac
      721 tccaataaaa caacagataa atcccatcaa aagtaaaagc caacccgtat tgatcgttag
      781 aataacgccg ataacaaaaa caaatgcaat catagctaaa atcatgtttc gaatcaatac
      841 aggagataga tttctgcggc caatgacatt ttcttcatac ttatatactt cagaatgagc
      901 cttttcataa tccatgtaat t
//
LOCUS       DAZULO010000239          879 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000239 DAZULO010000000
VERSION     DAZULO010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.250"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..310
                     /locus_tag="ACG8OA_002959"
     CDS             137..310
                     /locus_tag="ACG8OA_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977674.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          290 a          144 c          107 g          338 t
ORIGIN      
        1 tttttcggag aattaaaaaa tacaataaat aactttagtt actttaatgc gttcttactt
       61 tatttttata ttatcatttt tcttattcac ttctttggtt tatactattc ttataaataa
      121 aaacgaggta tgtcctatga tgaaaagatt gtactattca ttgatcataa cgattggcta
      181 tttgattgta tcaaatctag gaaatatggt atttggtatc agtaaagaat tcagttggac
      241 tactacatta tgggaatctc tattcttttt catcttcgta tttttgttac aaaattatcg
      301 aaaaaaataa acatgtgcca tctatctgtt ggcacatgtt ttaggattta tggtactttt
      361 tattcggcaa tccattttat ttttctactt cttcttaata cctctaacac ttttgattta
      421 taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga tagtaccacc
      481 ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata aaggacaaat
      541 aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa taaacatgaa
      601 cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct catacagttc
      661 tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta ttgtccaata
      721 taatcccagg catctaaact agcattatag aagagacaca catagaatct aaaataactg
      781 gttttaaatg tttcttacaa tcaaaaaaag ctgttaaatg cactccacac cttcatactc
      841 tatcattttt tataaacgat atatttttat tgtttttcg
//
LOCUS       DAZULO010000240          842 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000240 DAZULO010000000
VERSION     DAZULO010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.251"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            380..778
                     /gene="spxA"
                     /locus_tag="ACG8OA_002960"
     CDS             380..778
                     /gene="spxA"
                     /locus_tag="ACG8OA_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFS4977675.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          316 a          114 c          150 g          262 t
ORIGIN      
        1 aaataagcgt tataataaag aacagtatta tttaaaatga cagaaaacat tactattaaa
       61 gaaataaaaa acataaaaaa tcttaagaaa tcggtttcat ttagcttttg agataaaata
      121 atagtacagt tttatacgct gtttcatatg tttagaaaat ttgaattaga tttgaaaact
      181 ctaattttta aatgataaaa aaagcgagaa tgctggtttt actgtatatt aaccttagat
      241 aactttcatt tttttaaaaa aattatgaaa aaaaggtagc tatttaaaag ttgattatgt
      301 ataatagaaa cagatgggag attattatga tcttctgtta caaaaaagtg attcttatca
      361 cgaaaaggag tggtgcacgt tgttaacatt atatacttcc ccaagttgta cttcctgcag
      421 aaaggctcgt gcgtggttgc aagaacatca gattccattt gttgaacgta atattttttc
      481 agaaccattg aacagttctg aattaaaagc gattttacaa atgacagaag acggaacaga
      541 agaaatcatc tcgacacgtt ccaaagtatt ccaaaaactg aacatggatt tagatgatct
      601 gccgttacaa gaattattgg aacttgtgca aaacaatcct ggattgcttc gccgtccaat
      661 catgattgat gataaacgtc tgcaagttgg ttttaatgaa gatgaaattc gtcggttcct
      721 accgcgcgat gtacgacaat tagaattacg tcaagcacaa ttgatggctg gattatagaa
      781 ggataatacg aaacttggaa aagactggct ctttgtcaaa tgatgttggt aaagagaaat
      841 ct
//
LOCUS       DAZULO010000241          821 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000241 DAZULO010000000
VERSION     DAZULO010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.252"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..794
                     /gene="fabI"
                     /locus_tag="ACG8OA_002961"
     CDS             39..794
                     /gene="fabI"
                     /locus_tag="ACG8OA_002961"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFS4977676.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT          261 a          158 c          177 g          225 t
ORIGIN      
        1 gactatcaaa tttttatcag aaacaaagga gagtcattat gtcatttcta gaaggaaaaa
       61 aaatcgtcat tatgggcgtc gctaacaaaa aaagcattgc ttggggttgt gctgaagcaa
      121 tgttgaaaca aggggcagaa attatctata cttaccaaaa tgaacgaatg aaaaaatcat
      181 tagtcaaatt ggtcggcgaa gaagcattca ctgtagaatg tgatgtttct agcgacgaaa
      241 gcatcgaaaa agcaatgaat gccatcggtg aatatgctgg ggagatccac ggccttgtcc
      301 atgcagttgc ttacgccaac aaagaagaat tgtccggaaa tgtgtctgat atttccagag
      361 acggctatca attagctcaa gatatcagca gctactcttt aattgctgta tcaaaatacg
      421 cacaaccgtt tcttgcacca aacagcggga tcgttagcat gacttatcta ggttcagaac
      481 gcgcaatccc gaattataac atgatgggta ttgcgaaagc ttctcttgaa gctgctatcc
      541 gttatttggc agctgagttc tcgcctaaag gaattcgggt caatggaatc tcagctgggg
      601 caatcaaaac attagctgtc acaggtgtta aagactatca aaaactgatc cagttatctg
      661 aatctcgtac gcctgatcag caaggtgtga cgatcgaaga agtcggtaat gcttgtgctt
      721 tcttaatcag cccactttcc agcggaatca tcggtgatat tatctacgtt gacaaaggtg
      781 tccatctttc ataaactttt gttgcattct ttaaagtaaa a
//
LOCUS       DAZULO010000242          778 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000242 DAZULO010000000
VERSION     DAZULO010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.253"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            514..777
                     /locus_tag="ACG8OA_002962"
     CDS             514..777
                     /locus_tag="ACG8OA_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977677.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
BASE COUNT          307 a           86 c          137 g          248 t
ORIGIN      
        1 aattttttcg ttagtgtatt aatgggtgaa agtttaatcc taatactgat tttgtgttta
       61 attgttcatg aagtgaataa acaattgtga attaaaaaaa taaaaaccta gtataatcac
      121 tttaataaat tgttggtaga aggtgatcaa aatgggagta aacgaaatga tgaaagtcag
      181 attgagtttt taaatgatac cttcctcaga acttcaaaac gaattcaaag attcgttgac
      241 aaatcttgag caagatcatt taaaaaaata ttttctcttc atttgacgaa aaatgtttag
      301 aaatcttata taagtgaaga taaagtaatt ggtgtaatta attgtagaga atagcctttg
      361 agcaattgaa gcgatacaat tatttagaaa atgaacttcc tccagcatac tacatgattc
      421 cttacgggac gatgttaaag aagaaagcaa gaaattttag aagtattagt agataaacta
      481 atgggctttt ctcaatttaa gggagtagcc caaatgttta tcaaatatgg tgcagataca
      541 gtagcaacca tgtttacaga agaatatcaa gtagactatt atgaagtaat agataaatgt
      601 gtacattttg tttatgaaaa agtgtggaat tttatgaatt ttgcgataag tgaagaaatc
      661 ttacgagtac aagaaaacag aacaggaata tttaccagag acagaaagct taatttgatt
      721 gcgcattata tacggaatcg ctggtcatca ataccaaatt ttaattataa aaaataat
//
LOCUS       DAZULO010000243          773 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000243 DAZULO010000000
VERSION     DAZULO010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.254"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACG8OA_002963"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACG8OA_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS4977678.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="ACG8OA_002964"
     CDS             complement(481..675)
                     /locus_tag="ACG8OA_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977679.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          229 a          162 c          134 g          248 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaag acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag taaggtactg ttctatgggg cgcttacgtt aaaaatgttt tgc
//
LOCUS       DAZULO010000244          752 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000244 DAZULO010000000
VERSION     DAZULO010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.255"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(91..>752)
                     /locus_tag="ACG8OA_002965"
     CDS             complement(91..>752)
                     /locus_tag="ACG8OA_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFS4977680.1"
                     /translation="RIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQ
                     PLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLT
                     DVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQY
                     QFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEA
                     A"
BASE COUNT          204 a          176 c          144 g          228 t
ORIGIN      
        1 ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc ctcggatctg
       61 attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc
      121 gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc
      181 atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc
      241 tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgacaga
      301 tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac tcaatgaacg
      361 agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt cctttccccg
      421 gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa aggcttcttt
      481 taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc gattaaacaa
      541 atcaagtatt aagcagatat aatgccactt tttccccacg cgaacataag taagatccgt
      601 aacgatggct tccaatggct gttcttgtgt aaaggtccga tctaatacat tcgctgtttt
      661 cgcttcatta caagctgttc gttgcccttt aaaatgagcg atcgtatagg tagatgtcaa
      721 tccgcggcgt ttcatgattc gaccgattcg gc
//
LOCUS       DAZULO010000245          733 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000245 DAZULO010000000
VERSION     DAZULO010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.256"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>733
                     /locus_tag="ACG8OA_002966"
     rRNA            <1..>733
                     /locus_tag="ACG8OA_002966"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DAZULO010000246          712 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000246 DAZULO010000000
VERSION     DAZULO010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.257"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..446)
                     /locus_tag="ACG8OA_002967"
     CDS             complement(<1..446)
                     /locus_tag="ACG8OA_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4977681.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DAZULO010000247          703 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000247 DAZULO010000000
VERSION     DAZULO010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.258"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          297 a          106 c          122 g          178 t
ORIGIN      
        1 attccttagg cagcttttgc tgtgtagaaa ggaataaata ataacgaaag ttcaaaagct
       61 aatgccgact attctagaca ttaaagaaga cgaaaacgaa cgataaagaa agaaaagtta
      121 tgaataatcg tttattgaaa aaaacttgaa aaaatacaaa aaattcgttg accaagctaa
      181 atttagttgg tatattatta cttgtccaaa agacagaaga aaatatttat tcaaaaacat
      241 aacaaaaaag ttattgacat gttactaacg acatgataag ataactgagt cgttaaaaaa
      301 gaaccacttg cgaagcttga ttctatcgaa aaaaacttca aaaaagttct tgacaaataa
      361 caaacgataa gttatgataa atgagttgcc gaaagatttc gaaaaacgaa atcgaaaaaa
      421 acttttaaaa aagagttgac aatcgcttgt ctctctgata tgatataaaa gttgctacgg
      481 cagccaaaaa tgaatcggga aacactctcg atgaagtaga cctttgaaaa ctgaacaaag
      541 taagacaaac caaatgtgta gggcgtcttg attcaattca agacaacaaa catttttaac
      601 aagcaagcaa tatgctagca aacaaattga gcttaacaat cgcaagattg ttcgaacttt
      661 ttatgagagt ttgatcctgg ctcaggacga acgctggcgg cgt
//
LOCUS       DAZULO010000248          674 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000248 DAZULO010000000
VERSION     DAZULO010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.259"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>674
                     /locus_tag="ACG8OA_002968"
     CDS             <1..>674
                     /locus_tag="ACG8OA_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS4977682.1"
                     /translation="LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIK
                     GAHFFDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGI
                     ILSVIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFM
                     APKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMI
                     FIPRLG"
BASE COUNT          212 a           84 c          129 g          249 t
ORIGIN      
        1 ttgaatattg caatctctgt agctttgatg cagacattat attttgatga taaatatgtt
       61 ggcagagtag tcggcggaac actgtcattt gtgttgatag gtttagtaat ttatttaaga
      121 ataatgataa agggtgctca tttttttgat aaaaaacagt ggagatacgc tttaaaaatc
      181 tctttgccgc taatcccaca ttccttagca aatatagttt tagcacaatt cgatagaatt
      241 atgattaatt cttatttagg tcctgctgcg gggggaatat attcctacat tagtaacata
      301 ggtattattc taagcgtaat atggatgtct actaataacg cctgggttcc atggttctat
      361 ggtgagatga gtaaaaataa tgaaaaaaaa gtgaagaaag ttagtaacta ttatttagta
      421 gtctttactt tgattacgat gattgtaatg gtagtttcga ttgatttagc aaaatttatg
      481 gctccgaaag actattattc tgggttatct ttagtggttc cgataacctt aggctatttc
      541 tttcagtttt tatatagttt accagttaat gctgaatttt atgagaaaaa aacatcgttt
      601 attgcgattg gtacagtagc aagtgctata gttaatatac tattaaacat gattttcata
      661 cctaggttag gaat
//
LOCUS       DAZULO010000249          662 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000249 DAZULO010000000
VERSION     DAZULO010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.260"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>662
                     /locus_tag="ACG8OA_002969"
     CDS             <1..>662
                     /locus_tag="ACG8OA_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4977683.1"
                     /translation="YKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPI
                     SCSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNE
                     FGHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLF
                     KSITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFN
                     SV"
BASE COUNT          221 a          112 c          136 g          193 t
ORIGIN      
        1 gtataagaaa aacaaatcaa actgtggtag acgtccgctt gttttacccg aggaacaatc
       61 agaatatatt caaagaaagg ttgttcaagg atggacaccc gatgtgattg ttggtcgtgc
      121 agcgtttcct attagttgtt ctgctcgtac catttatcgt atgttcaaaa aggggctatt
      181 tgattcttct gacttaccga tgaaaggcaa gcgtaaaccg aatggacatc aagaaagacg
      241 tggaaaacaa actttccgcc gctctattca tgaacgtgaa aaggattata gccaattctc
      301 aaatgagttt ggtcaccttg aaggtgacac tatcgtaggt ctgaaacata aaagtgctgt
      361 aattacctta gttgaacgat tatcaaaagt tatcatcaca ttgaaaccgt gtggtagaca
      421 agcgattgat attgaaaaaa aattaaatca atggtttgaa tctgtaccga aaaacctatt
      481 caaatccatc acttttgatt gtggaaagga attttcaaat tggaaacaga tcagtaatgt
      541 caatgatatt gccatttatt tcgctgatcc aggaacgccg tctcaaagag gcctaaacga
      601 gaattctaac ggattgttac gtagagatgg tttattgaaa tctatggatt tcaattcagt
      661 ag
//
LOCUS       DAZULO010000250          643 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000250 DAZULO010000000
VERSION     DAZULO010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.261"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..>643
                     /locus_tag="ACG8OA_002970"
     CDS             26..>643
                     /locus_tag="ACG8OA_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFS4977684.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKE"
BASE COUNT          234 a          108 c          129 g          172 t
ORIGIN      
        1 gaaaaagata gcaggaggaa tgacgatgaa tcattttaaa ggaaagcaat ttcagcagga
       61 tgtgattatt gtagccgtgg gctactatct tcgttataac cttagctatc gtgaagttca
      121 agaaatctta tatgatcgtg gcattaacgt ttctcatacg acgatttatc gttgggtgca
      181 agaatatggc aaactactct atcaaatttg gaaaaagaaa aataaaaaat ccttttattc
      241 atggaaaatg gatgaaacgt acatcaaaat taaaggaaaa tggcattatt tgtatcgagc
      301 catcgatgca gatggtttaa ccttggatat ttggttacgt aaaaaacggg acacacaagc
      361 agcctatgct tttcttaagc ggttagtgaa gcagtttgat gaaccgaagg ttgtagtcac
      421 agataaagcc ccctctatta caagtgcctt taagaaacta aaagaatacg gcttttatca
      481 agggacagaa catcgtacca ttaaatacct gaataatttg attgaacaag accatcgtcc
      541 agtaaagaga cgcaataaat tctatcgaag tttacgcact gcctctacca cgattaaagg
      601 catggaagcc attcgaggat tatataagaa aacccgaaaa gaa
//
LOCUS       DAZULO010000251          637 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000251 DAZULO010000000
VERSION     DAZULO010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.262"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..511)
                     /locus_tag="ACG8OA_002971"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="ACG8OA_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
BASE COUNT          202 a          121 c           99 g          215 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaa
//
LOCUS       DAZULO010000252          635 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000252 DAZULO010000000
VERSION     DAZULO010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.263"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..270)
                     /locus_tag="ACG8OA_002972"
     CDS             complement(<1..270)
                     /locus_tag="ACG8OA_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977685.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="ACG8OA_002973"
     CDS             complement(276..>635)
                     /locus_tag="ACG8OA_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS4977686.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DAZULO010000253          629 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000253 DAZULO010000000
VERSION     DAZULO010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.264"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..629
                     /locus_tag="ACG8OA_002974"
                     /pseudo
     CDS             1..629
                     /locus_tag="ACG8OA_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          248 a          105 c          120 g          156 t
ORIGIN      
        1 atgggtaaag caatctaaaa cgagtggttc attcaaagaa aaagataatc ttacgcctga
       61 acaaaaagaa ttgttagaac tacgtaaaag aaaccagcaa ttagaaatgg aaaatgatat
      121 tttaaagcaa gcagcgctga tattcggacg aagagacaag taatcgatgc gaataagcat
      181 ctttacccta tatcagcgat gtgcagaata ttaggtctat cacgtcagtc ctattattat
      241 caatcaaaac caaagaaaga cgaatcagaa cttgaagaag tagtcgctga agaatttatc
      301 cgcagccgaa aggcctacgg ctcaagaaaa ataaaaaaag ccttatcaaa acgaggcatt
      361 cagatcagcc gacgaaaaat tagtagaatc atgaaaaata gaggattaaa atcgagctat
      421 actgttgctt attttaaagt acatcattct acttgcaatg aagccaaaac gacaaacgta
      481 ttgaatcgta aattcttaag agacaaccca ttagaagcga tcgtaacaga cttgacttat
      541 gtacgagtcg ggaaaaaatg gaattatgtc tgtttcattt tggatctgtt caatcgagaa
      601 attctcggct attcttgtgg agaacataa
//
LOCUS       DAZULO010000254          625 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000254 DAZULO010000000
VERSION     DAZULO010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.265"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>625
                     /locus_tag="ACG8OA_002975"
     CDS             <1..>625
                     /locus_tag="ACG8OA_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFS4977687.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASI"
BASE COUNT          237 a           87 c          113 g          188 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatca
//
LOCUS       DAZULO010000255          584 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000255 DAZULO010000000
VERSION     DAZULO010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.266"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(74..205)
                     /locus_tag="ACG8OA_002976"
     CDS             complement(74..205)
                     /locus_tag="ACG8OA_002976"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977688.1"
                     /translation="MQSAKACGIAFGLAKWGAKNIENFQSVDIIIDSPQEILDLLEK"
     gene            complement(178..300)
                     /locus_tag="ACG8OA_002977"
     CDS             complement(178..300)
                     /locus_tag="ACG8OA_002977"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977689.1"
                     /translation="MHKPFPDPIQKAIDELGIKNKKRFILVIHYMTCKVRKLVG"
     gene            456..>584
                     /locus_tag="ACG8OA_002978"
     CDS             456..>584
                     /locus_tag="ACG8OA_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFS4977690.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTD"
BASE COUNT          207 a          104 c           81 g          192 t
ORIGIN      
        1 tggcaaaaat ttcaatcttt aaatggcgaa aaacaaagag cgtgagaccc tatccttatc
       61 tcacgctctt tgtctatttt tctaacaaat caagtatttc ttgaggagaa tcaataatta
      121 tatctacact ttgaaaattt tctatatttt tggctcccca tttagctaaa ccaaatgcta
      181 tcccacaagc tttcgcactt tgcatgtcat ataatgaatc accaatataa atcgtttctt
      241 attttttatg cccaattcat caattgcctt ttgtatgggg tccggaaaag gtttatgcaa
      301 cttagtatct gaagctgtaa tttgtatatc aaaatataga tttaacccgt ggtgctagtt
      361 aattttttcc tagaggaaaa tgtctaaaca tgttggtaaa tcaacaaaaa aacgcatata
      421 gtagtctcaa aactcccaag aaatgaggac tacatatgcg ttcatcaaaa tatactgaac
      481 attatacaga tacttttata acattcaagg aaattatgag aactgtttct aacatatacc
      541 acaattgtgt accagataag attaaaaacc gaagaaatac tgat
//
LOCUS       DAZULO010000256          560 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000256 DAZULO010000000
VERSION     DAZULO010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.267"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          187 a           88 c           87 g          198 t
ORIGIN      
        1 gaagagtatt atacaagcta aagcttttct tcattgccac tcaaatgagt ggctttttta
       61 tgtatccttt tatggattaa tgaaaggatg tttcacatag ttatacttct gtatatttga
      121 aaagttttac tttgattttt aaatagaaag acatttgggt taaattgtga gataataata
      181 aagaagagtt taaagcgcac cccaaaccac ttccccataa gtgtgttacg ctttaaactc
      241 ttttatattt gaagccatta aaaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttacaattg gagtggtagt taattagtga cttatttttg attttatagc actgatacta
      361 taaaatatag atatcatcat attacacaat cttaatacta acttaaaaaa tatctccttt
      421 cacaagtatg gtgataaaat tcgttccggg ctaccttttt aggtagccta ctttaatctt
      481 tatacctttc tggatcaacg aaagtatact ttatatagtc ataacgccga tgatcgctac
      541 gtgcgtccgg cacgtcagtc
//
LOCUS       DAZULO010000257          531 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000257 DAZULO010000000
VERSION     DAZULO010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.268"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..455)
                     /locus_tag="ACG8OA_002979"
     CDS             complement(<1..455)
                     /locus_tag="ACG8OA_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4977691.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKS"
BASE COUNT          150 a           88 c           84 g          209 t
ORIGIN      
        1 gatttgataa tcccttttgg ttgtctatga agcttaattc ttactaatga cttcaattta
       61 ggaactttga tgaatttact atcaatcaaa gcaactgtac cattttgatt attcgttgta
      121 taactctgaa caggattttt cttacttttg aaacgtggaa atccaacgga tttatcccga
      181 aaaaaattct tgtatgcttt atctaaatta agttgggcat tggctaaagc aaggctatca
      241 atttctttca aaaacggaaa ttctttcttg tatttagctg gtgtcggaaa agtcattttt
      301 ttagaagaat catttttaac ttcttcatac gccttttttc ggtcatcaag cattagattg
      361 tagaccttac ggacacaacc aaaagatttg gcaaagaaga tttcttgttc ctctgttgga
      421 tagattctga atttgtatgc ttttagttgt tccataaagt tcacctactt tctatttatg
      481 attgtttcct tgattttgga tatacttctt aatcacatca attggtgcgc c
//
LOCUS       DAZULO010000258          526 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000258 DAZULO010000000
VERSION     DAZULO010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.269"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..354
                     /locus_tag="ACG8OA_002980"
     CDS             <1..354
                     /locus_tag="ACG8OA_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS4977692.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
BASE COUNT          209 a           75 c          115 g          127 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacaga
//
LOCUS       DAZULO010000259          489 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000259 DAZULO010000000
VERSION     DAZULO010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.271"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          162 a           96 c           58 g          173 t
ORIGIN      
        1 agaatgctaa atcactctac aataacggcg aaagcacaaa aaataaaaaa attattacgt
       61 tattaaaaaa acagatgaaa gatcaggttt tattagatct cattctttca tcacttgaag
      121 ggatttaatg aactactttt tcctctttgt cgtttttata tattcgtatt ccttcaaaca
      181 gtaggatagc ctctttcaat gcttgatatt tgaactgtcc gttacttaaa aatttagatg
      241 tgccgccctc ttcctagaac atattctttt ttactttttc attaattgac agaccaatcg
      301 aacgaaactg taaatttcga taggataatg attggtcaaa ctcattcatt ttcaagacat
      361 ctcgattcat attcaagata ggaatctttt tatcaacatc actttattcg tattttcctc
      421 taccataaaa gcttcacgct ccacaaaatt gttttataca ctactattca gcccattcaa
      481 cgaccaatg
//
LOCUS       DAZULO010000260          443 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000260 DAZULO010000000
VERSION     DAZULO010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.272"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..>443
                     /locus_tag="ACG8OA_002981"
     CDS             22..>443
                     /locus_tag="ACG8OA_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4977693.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQA"
BASE COUNT          160 a           84 c           91 g          108 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
      241 gctgaggaaa aagaatacat tgaaaaaaga tcaactgaag gttggacccc agacgtcatt
      301 attggccgtg cagagcgaac cttctcttgt tcagtaagta ccctctatcg ccggtttaag
      361 acgggagaat tcaatgtttt acatttaccg atgcaaggaa aacgaaaacc aaatggttat
      421 aaggaaaaac gtggaaaaca ggc
//
LOCUS       DAZULO010000261          374 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000261 DAZULO010000000
VERSION     DAZULO010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.274"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..281
                     /locus_tag="ACG8OA_002982"
     CDS             78..281
                     /locus_tag="ACG8OA_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HFS4977694.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
BASE COUNT          150 a           41 c           78 g          105 t
ORIGIN      
        1 gatatttaat attacaagca atcttaagaa attcgaaaag gaaaaaatga ttatgaaaaa
       61 gaaagaaaaa gaaagtattg aaacattgtg ttattctagg aatattagga acttgtctag
      121 ctggcattgg tacaggaata gacgttgacg cagctactta ctatggaaat ggtctttatt
      181 gtaacaaaga aaaatgttgg gtaaattggg gacaatcatg gtcagaaggg ttaaaacgtt
      241 ggggagataa tctttttggt agttttagtg gaggacgata atgacttcaa taaacattga
      301 tcacacaaaa aaagtcatac atgaattata caatagttta atgacacgac ctaatcagac
      361 gaaagcatta ttag
//
LOCUS       DAZULO010000262          349 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000262 DAZULO010000000
VERSION     DAZULO010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.277"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>349
                     /locus_tag="ACG8OA_002983"
     CDS             <1..>349
                     /locus_tag="ACG8OA_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HFS4977695.1"
                     /translation="DIWLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRK
                     LQSVGLYTKTEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKK
                     NRRNGTLFGFSVST"
BASE COUNT          118 a           72 c           71 g           88 t
ORIGIN      
        1 agatatctgg ttacgaaaga aacgggatac gcaagcagcc tatgctttct taaaacgact
       61 ccataaacag tttggtgagc cgaaagcaat tgtgaccgat aaagcacctt ctcttggctc
      121 cgcctttaga aagttacaga gtgtgggttt atatactaag acagagcacc gaactgtgaa
      181 gtatcttaac aatttaatag aacaagacca tcgacctatt aaacgacgaa ataaatttta
      241 tcaaagtctc cgtacagcct cttccacgat taagggcatg gagaccattc gaggaatata
      301 taaaaagaac cgaagaaatg gaacgctctt cggcttttcg gtgtctact
//
LOCUS       DAZULO010000263          321 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000263 DAZULO010000000
VERSION     DAZULO010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.278"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACG8OA_002984"
     CDS             complement(<1..>321)
                     /locus_tag="ACG8OA_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4977696.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAZULO010000264          320 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000264 DAZULO010000000
VERSION     DAZULO010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.279"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          112 a           39 c           54 g          115 t
ORIGIN      
        1 aagagtatta tacaagctaa agcttttctt cattgccact caaatgagtg gcttttttat
       61 gtatcctttt atggattaat gaaaggatgt ttcacatagt tatatttctg tatatttgaa
      121 aagttttact ttgattttta aatagaaaga catttgggtt atattgtgag ataataataa
      181 agaagagttt aaagcgttcc ccaaaaacca ctgccccata agtgtgttac gctttaaact
      241 cttttatatt tgaagccatt aaaaagcata ccatattttt gaaaaaaagt gagaaaaaag
      301 gcttataatt ggagtggtag
//
LOCUS       DAZULO010000265          309 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000265 DAZULO010000000
VERSION     DAZULO010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.281"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(77..>309)
                     /locus_tag="ACG8OA_002985"
     CDS             complement(77..>309)
                     /locus_tag="ACG8OA_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4977697.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           94 a           46 c           57 g          112 t
ORIGIN      
        1 gatgttggtg aagttcacca acatcaaaaa gtatagagcc gatagaagga ctgaccttct
       61 gtctcagatt tttttgttat ttcacttgaa tcaattgatt gatcaaaaaa atcctgattt
      121 tcatgttctc aaatgattta aatccataag ataatcgttt cagtgtcttg atatggggaa
      181 tcttggcctc tattttgcca ttagaatacg ggtaaatcat agcattgtaa ataccttctt
      241 catacgttaa aaggttttga agtttcgtgc gaagttgtgc atcaagtgtt tcaggaagat
      301 tttttaata
//
LOCUS       DAZULO010000266          305 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000266 DAZULO010000000
VERSION     DAZULO010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.283"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG8OA_002986"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG8OA_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFS4977698.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           95 a           45 c           50 g          115 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgtt
//
LOCUS       DAZULO010000267          299 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000267 DAZULO010000000
VERSION     DAZULO010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.284"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>299
                     /locus_tag="ACG8OA_002987"
     CDS             <1..>299
                     /locus_tag="ACG8OA_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4977699.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT          102 a           63 c           54 g           80 t
ORIGIN      
        1 acgattgtcg gcatccacca taaaagtgcc gtcatcacac tcgttgagcg actttcaaaa
       61 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
      121 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      181 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      241 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DAZULO010000268          299 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000268 DAZULO010000000
VERSION     DAZULO010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.285"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..299
                     /locus_tag="ACG8OA_002988"
                     /pseudo
     CDS             1..299
                     /locus_tag="ACG8OA_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          119 a           59 c           56 g           65 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcag
//
LOCUS       DAZULO010000269          282 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000269 DAZULO010000000
VERSION     DAZULO010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.denovo.286"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>282
                     /locus_tag="ACG8OA_002989"
     CDS             <1..>282
                     /locus_tag="ACG8OA_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4977700.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLE"
BASE COUNT          114 a           52 c           45 g           71 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aa
//
LOCUS       DAZULO010000270        31624 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.guided.022, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000270 DAZULO010000000
VERSION     DAZULO010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.guided.022"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..380
                     /locus_tag="ACG8OA_002990"
     CDS             <1..380
                     /locus_tag="ACG8OA_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4977701.1"
                     /translation="QAMKDLLAHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERW
                     EWFIGKNRGALRLTTQPEPYSFERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQE
                     AKLTEKHEKLIEQQHLAMEDLIT"
     gene            377..667
                     /locus_tag="ACG8OA_002991"
     CDS             377..667
                     /locus_tag="ACG8OA_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977702.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            664..885
                     /locus_tag="ACG8OA_002992"
     CDS             664..885
                     /locus_tag="ACG8OA_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977703.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            927..1706
                     /locus_tag="ACG8OA_002993"
     CDS             927..1706
                     /locus_tag="ACG8OA_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFS4977704.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            1767..2267
                     /locus_tag="ACG8OA_002994"
     CDS             1767..2267
                     /locus_tag="ACG8OA_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS4977705.1"
                     /translation="MEMKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEE
                     YAIHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENK
                     EDIHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHG
                     VFEYCQ"
     gene            2323..2961
                     /locus_tag="ACG8OA_002995"
     CDS             2323..2961
                     /locus_tag="ACG8OA_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFS4977706.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            3033..3428
                     /locus_tag="ACG8OA_002996"
     CDS             3033..3428
                     /locus_tag="ACG8OA_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4977707.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            3412..5865
                     /locus_tag="ACG8OA_002997"
     CDS             3412..5865
                     /locus_tag="ACG8OA_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4977708.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            5862..7889
                     /locus_tag="ACG8OA_002998"
     CDS             5862..7889
                     /locus_tag="ACG8OA_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS4977709.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            7886..8908
                     /locus_tag="ACG8OA_002999"
     CDS             7886..8908
                     /locus_tag="ACG8OA_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFS4977710.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            8925..9854
                     /locus_tag="ACG8OA_003000"
     CDS             8925..9854
                     /locus_tag="ACG8OA_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4977711.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            10168..10215
                     /locus_tag="ACG8OA_003001"
                     /pseudo
     CDS             10168..10215
                     /locus_tag="ACG8OA_003001"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10231..11031
                     /locus_tag="ACG8OA_003002"
                     /pseudo
     CDS             10231..11031
                     /locus_tag="ACG8OA_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            11269..11505
                     /locus_tag="ACG8OA_003003"
     CDS             11269..11505
                     /locus_tag="ACG8OA_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977712.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            11512..13464
                     /locus_tag="ACG8OA_003004"
     CDS             11512..13464
                     /locus_tag="ACG8OA_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFS4977713.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(13536..14033)
                     /gene="dfrG"
                     /locus_tag="ACG8OA_003005"
     CDS             complement(13536..14033)
                     /gene="dfrG"
                     /locus_tag="ACG8OA_003005"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFS4977714.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            14308..15435
                     /locus_tag="ACG8OA_003006"
                     /pseudo
     CDS             14308..15435
                     /locus_tag="ACG8OA_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            15533..15721
                     /locus_tag="ACG8OA_003007"
     CDS             15533..15721
                     /locus_tag="ACG8OA_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFS4977715.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(15779..16132)
                     /locus_tag="ACG8OA_003008"
     CDS             complement(15779..16132)
                     /locus_tag="ACG8OA_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4977716.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            16663..17085
                     /locus_tag="ACG8OA_003009"
     CDS             16663..17085
                     /locus_tag="ACG8OA_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4977717.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            17082..17312
                     /locus_tag="ACG8OA_003010"
     CDS             17082..17312
                     /locus_tag="ACG8OA_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4977718.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            17810..18010
                     /locus_tag="ACG8OA_003011"
     CDS             17810..18010
                     /locus_tag="ACG8OA_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFS4977719.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            18037..19230
                     /locus_tag="ACG8OA_003012"
     CDS             18037..19230
                     /locus_tag="ACG8OA_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4977720.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(19298..20863)
                     /gene="guaA"
                     /locus_tag="ACG8OA_003013"
     CDS             complement(19298..20863)
                     /gene="guaA"
                     /locus_tag="ACG8OA_003013"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS4977721.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(20922..21845)
                     /gene="coaA"
                     /locus_tag="ACG8OA_003014"
     CDS             complement(20922..21845)
                     /gene="coaA"
                     /locus_tag="ACG8OA_003014"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFS4977722.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            22041..22646
                     /locus_tag="ACG8OA_003015"
     CDS             22041..22646
                     /locus_tag="ACG8OA_003015"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4977723.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            22954..23970
                     /locus_tag="ACG8OA_003016"
     CDS             22954..23970
                     /locus_tag="ACG8OA_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4977724.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            24111..25412
                     /locus_tag="ACG8OA_003017"
     CDS             24111..25412
                     /locus_tag="ACG8OA_003017"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFS4977725.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            25458..26120
                     /gene="deoC"
                     /locus_tag="ACG8OA_003018"
     CDS             25458..26120
                     /gene="deoC"
                     /locus_tag="ACG8OA_003018"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFS4977726.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            26141..26539
                     /locus_tag="ACG8OA_003019"
     CDS             26141..26539
                     /locus_tag="ACG8OA_003019"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFS4977727.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            26605..27687
                     /locus_tag="ACG8OA_003020"
     CDS             26605..27687
                     /locus_tag="ACG8OA_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4977728.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            27899..29461
                     /locus_tag="ACG8OA_003021"
     CDS             27899..29461
                     /locus_tag="ACG8OA_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4977729.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            29454..30587
                     /locus_tag="ACG8OA_003022"
     CDS             29454..30587
                     /locus_tag="ACG8OA_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4977730.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            30587..31543
                     /locus_tag="ACG8OA_003023"
     CDS             30587..31543
                     /locus_tag="ACG8OA_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4977731.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
BASE COUNT        10474 a         5475 c         6416 g         9259 t
ORIGIN      
        1 cccaagccat gaaagattta ttggcacatc aacaggcgga aaaaacagcc tttgaaatta
       61 tcaatcgcta tatccgcttt gcagataaag acgacactaa acgtcggagt gattggaaaa
      121 ctaatgaacg ttgggaatgg tttatcggta aaaatcgtgg agcattacgc ctaaccacac
      181 agccagagcc ttattctttt gaacgaacat tgaactggtt acatcatcaa gtcgccccta
      241 cattaaaaat cgcaagcatt ttagatgttc tcaacggtac aaccattatc tccaccatga
      301 ttcaggaagc caaactgaca gaaaaacatg agaaattaat tgaacaacaa catctagcca
      361 tggaggattt gattacatga gccttttaac aaaagaatta aagaagttag ggtttcagtg
      421 tggcattgag tttcaagcgt atatacaaaa tacagggaaa tatacgtctc tcattattga
      481 gggaaaacgg caagctggtg ataccatcta tacctatgat ttttacaagg tgagatttta
      541 taataattat accaatcgag taaccgtcta tggagagcat ttaacccctt ttcaattatt
      601 aaggcgtgta aaaagctata tctattatcg agaaaaatat cttaaggaaa ggaggacaat
      661 cacatgaatt ttgggcagaa tttgtacaac tggttcttgg ataatgcaca gtcattggta
      721 ttgttagcga ttgttgtcat tggactgttt ttaggtttta aacgagaatt ttcaaaactc
      781 attggcttct tgattattgc tctaatcgcc gtcggacttg tgttcaatgc agctggcgtg
      841 aaagatgtgt tattaaactt atttaaccga atcattggtg cttaacagat atttttcatg
      901 ataaaatagg aagtatatat tttgttatga aaggatttat gaatatgttt acaaatgatc
      961 aatttaaatc tcttttaatt aaaacattag aaagaaagag ctcttcaaat tattatgaag
     1021 gaggtaacga gattgtttct aaaatgaaaa tatcagaggt aactttagat gagactgacg
     1081 attttactta ttataaagga tataaaaagg gctggaatac gctttgcaca tatcttaaaa
     1141 taagagttcc ttttgatgat ttagactttt ttgaaagtca taaagatttg attacccaga
     1201 cagcaagctc catatatgat aaacaaggtg ataatgtatt agtggatact attttagtac
     1261 cacttccaga gaattacgaa gtaattaact ttagtcagtt gaaaattagt gatgtggttt
     1321 cacaagctat tgaagacgct gagtctttta tgagtaatgg tgaatatcaa cgtgcctttg
     1381 acagagttca cactgctttt catggatatt taattgaaat actaaaaaaa tatgaaatta
     1441 cagttcctag agacgaaaat ctatctaaat tatatagtag gatacaacaa ttaattgaaa
     1501 aagaaattca acctactgaa cttgctgata tagtgaagac aacgataagg tcatcaaatg
     1561 gaatgattag ctctcttaat gaagcaagaa ataggcattc acttgcacac cctaatacaa
     1621 atattattgg aaagagagaa gcaaaactaa taataggtat ttcaagtaca gttacagatt
     1681 atattagcgg ataccttgat aagtagcgtc attaactgat tgctactttt tttatgacta
     1741 tttttataaa aaggatgtga taagaattgg aaatgaaagt ctatgtggca aatttaggac
     1801 ggtataatga aggtgaatta gtcggagcat ggtttactcc accaattgat gaggaagaaa
     1861 tggcagaaag aatcggttta aatgaggact acgaagaata tgcaatacat gattttgagc
     1921 ttccttttga tgtggacgaa tacacgccga ttagtgaaat caaccgtcta tgtgaagcca
     1981 ttcaagaaat cgaaggtaca cctatttata atgaactcaa agaaattcaa ggcatgtggt
     2041 ttagtagctt ggaagaatta ctggaaaaca aagaggatat tcattgttat agtgattgtg
     2101 attcgatgga agatgtcgcc cgttattacg tggaagaaac aggacagctt ggtgaagtcc
     2161 cttccaattt acaaaattat attgactacc aatctttggg acgtgatatg gaaatagagg
     2221 gtaattacct agttacctct catggggtat ttgaatattg tcagtaagtt taacaatccg
     2281 tgttataatt aaattaataa atagcacgga gaggtataaa ttatggtaaa tgtatttgaa
     2341 aatcttaaac tattaaaact agacatgaaa gataaaggct gggtgataga ttccttttac
     2401 tttcgttaca aacagcaaaa ttatattgta cttgttaagc tatttgaaaa agaggaaaaa
     2461 gttccagaat atgctctgtt aaaactagaa tttttaaagg aaaacgattt ttcagatatg
     2521 ctagctgtct atgctaattc ggtaaaatta tatacagaca cgaaaacaat aagagaatat
     2581 tttggtatcg aatacagtag taacttggga gatgttttat ttcaatttag ccaaactctt
     2641 gctcgattta ttccgacaga agttagtgag aagaaaaatg aagaccaaaa agaagccatg
     2701 tgtttctcat taagtcaaag tgattcagaa gatcccagaa aaaaatattg tttttcagtt
     2761 agaagaaatc ctttaaaggg aaatggtgag ctgggaaaac gaagtccttt taatgacaat
     2821 aaaacgagat tgtatagacc cagcttatat gaacgattag ggtcagatac caatttaagt
     2881 ttcagatatt caatgaatcc agatgatgaa gaaactgatg aaggtattat tgcaaaatgg
     2941 acaaaaaata aaattgaata gttaaagcat tgaaagagca atcacctatt ttgattgttc
     3001 tttttctatt tttacggaaa ggacgtgaag acatgaaaaa aataagaagt tacaccagta
     3061 tttggtcagt agaaaaagtg atttatgcga ttaatgattt acaactccct ttcccaatta
     3121 cctttacgca aatgacatgg tttatcgttt ctttgtttgt cgtcatactc tttggcagtt
     3181 taccaccact ttctctgatt gacggagcac tactcaaata cttagggatt cccattgttc
     3241 tcactgggtt catgtgccaa aagacctttg acggaaagaa accctatgga tttctaaagt
     3301 ctatcattac ctatgctcta cgccctaaag taacctacgc tggcaaacaa atacgattaa
     3361 aaaaagaaac aatgaatgaa tcgattacga ttgtaaggag tgaacaacta gatggcatat
     3421 ccgattaaat atatggaaga taatctcgta tttaatcatg atggcgaagt attcgcttat
     3481 tatgaattaa ttccctataa ttacagtttt ttaagttccg atgaaaaaat tcaagttcat
     3541 gataatttct gtcaattgat tgcccaaaat cgtgatggca agattcatgc tctgcaacta
     3601 gcaaccgaat caagtattcg agccacacag gaacaatcga aaaaagaaat cactggacga
     3661 ttaaaagaga tagcttacga aaaaatagat gaacagaccg aagcgttaat ttctatgatt
     3721 ggtgacaatc aagtagacta tcgctttttt attggtttta aactctacct caatgaacag
     3781 gaagtatccg taaaaaatgt tgggaaagaa attcaaaaag catttgcgga attctttcat
     3841 ggtgtgaatc acaaattaat gggcgatttt gtttctatgc ctatggcgga aatcgaacgc
     3901 ttttctaaga tggaacagtt gttagaaagt aaattatcca gacgctttca gattcgtaaa
     3961 ttggagaaag atgattttgg ttatattatt gaacatcttt acgggcaaac aggtgtccct
     4021 tttgatgact acgactacca tttaccgaaa cgatacgaag ataaaaaagt gttggtaaag
     4081 aaatacgata ttctcaaacc cactcgctgt ttaatcgaag aaaatcaacg ctatttacgg
     4141 attgaacagg aagataccac tacttatgtg gcgtatttta caattaatag tattgttggt
     4201 gaattggatt tccctaatag tgaaattttt tattaccagc aacagcagtt tactttcccc
     4261 gttgatacca gtatgaatgt tgaaattgtc gccaataaaa aagccttatc taccgttcgc
     4321 aataaaaaga aagaactgaa agatttagat agccacgcat gggaaagcga caacgaaacc
     4381 agtactaatg tggtggacgc tttagaatct gtcgatgaat tagaaagtaa tttagaccaa
     4441 accaaagaat ctatgtacaa actaagctat gtggtacggg tatcagcaaa gaatttggac
     4501 gagctgaaac aacgttgtga tgaagtaaaa gatttttatg atgatctgtc tatcaaatta
     4561 gtacgacctt ttggcgatat gctaggcttg cataatgaat ttattccctc aagcaagcga
     4621 tatatcaatg actatattca atatgtcacg tcggattttt tggctggtct tggtttcggt
     4681 gctacacaaa tgattggtga aaattctggt atttacgtcg gttacaatct cgatacaggc
     4741 agaaatattt atctccaacc tagccttgca agtcaaggaa ttaaaggctc tgtaacaaat
     4801 gcattagcgt ccgcttttat tggttcatta ggtggcggaa aatcattctc taacaatatg
     4861 ctggtctatt attcagtact ctttggcgga caggcaacca ttgttgaccc aaaagctgaa
     4921 cgtggtgaat ggaaagaaac cttacctgat attgcccatg aaatcaatat tgttaatctc
     4981 acgagtgaga acgacaataa agggttactt gacccttatg tgattttaaa actcccgaaa
     5041 gattcagaaa gccttgccat tgatattctc acgttcttaa caggtatttc cagccgagat
     5101 agcgagaagt ttcctgtcct gcgtaaagct attcggagtg taactcaaag caaacaacga
     5161 gggttgttat tggtgattga tgaattgcga aaagaagata cgcccatcag caatagtatt
     5221 gctgaccata tcgaaagctt tgtcgattat gattttgccc atctgttatt cagtgatggc
     5281 agtgtcaccc aatccattag cttggaaaaa caactcaata ttattcaagt ggcagatttg
     5341 gtcttacccg attccaatag ctcttttgaa gaatacacta ccatggaatt attaagtgtg
     5401 gcaatgctga ttgtcattag tacctttgct ttagacttta tccactctga ccgttccatt
     5461 tttaaaattg ttgaccttga tgaggcttgg agttttttac aagtggcaca aggaaaagct
     5521 ctatctatgc gattggttcg agcaggacgt gccatgaatg caggggtcta tttcgttact
     5581 caaaacgcgg acgatttact agatgaaaag atgaagaata atattggctt gaaatttgcc
     5641 tttcgttcca ctgacctagt ggaaatcaaa aagaccttag aattttttgg tgtggacaag
     5701 gaagatgaaa acaatcagaa acgcttacgt gaattagaaa atggacaatg tttagtttca
     5761 gacatctatg gtcgtgtagg ggttatgcag tttcacccga tttttgaaga gttgcttcat
     5821 gcctttgata ccagaccgcc tgtgcgaaat gaggtggaag aatgacacaa aaacaaaaga
     5881 aatttcttca ctatagttgg ataacgctcc tttgtgtggg cgttatttta cttttattag
     5941 gcacgctggg caatgcagta caagcgacag gcttagttga tgaaaccatt gatacaagca
     6001 atgaatactc caagtatggt ttgaatcaat atcagcttga ttattatgtg gacaatagct
     6061 ggggctggct tccatggaat tggagtgatg gcataggaca atcggtgatg tatggattgt
     6121 atgccattac taattttatt tggacaattt ctctttatct atcgaacgcc acaggttatt
     6181 tggtgaaaga agcctatgcc ttagatttca ttagtcaaac atcccaagca attggcgaaa
     6241 acatgcagac gattgcaggc attacaccga atggcttttc cagttcaggg ttttatgtcg
     6301 gtttcctcct tctttttatt ctcattgttg gaatctatgt tgcctataca ggattggtta
     6361 agcgagaaac gacgaaagtg atacgagctg ttttaaactt tgtcgtggtg tttattctat
     6421 cggcttcctt tattgcgtat gcacccgatt atattgcgaa aatcaatgat ttttccaaag
     6481 atgtcagtac cgccagtctg aatattggga caaaaattgt tcttcctaac acggatagtc
     6541 aagggaaaga cagtgtggac atgatacgtg acagcttatt ctctgttcaa gtaaagcaac
     6601 catggctatt gcttcaatat ggctccactg atgtagaagc gttaggggaa gaacgtgtgg
     6661 aaagtttatt gtctaccagt ccagatacaa acaatggtga agatagagaa aatgtagtga
     6721 ttgaagaaat tgaagatcat gacaatacct atttaaccct accgaagacg atttccagat
     6781 taggaacagt cttctttttg tttgtattca atattggtat ctcgattttt gttttccttc
     6841 taacaggaat catgattttt tcgcaagtgc tatttattat tttcgctatg tttttgccaa
     6901 tcagtttcct attaagtatg ttgccgagtt ttgatggcat gggcaggcat gccattacca
     6961 agttatttaa tgtgattatg acaagggctg gtattacctt gattattacc gttgccttta
     7021 gtatttccac tatgctttat tcgctgtcag caagttctcc ctttttcatg attatgtttt
     7081 tacaaattgt gacctttgca gggatttatt ttaaactggg cgatttaatg agcctattca
     7141 ctttacaaag caacgactct caaaacatgg gcagacaagt gtttcgcaga cctcgtcaaa
     7201 tgatgaatcg acaaaccaga agatttcaac gcagtatgag taagattttt cgtggaaaag
     7261 cagggtcaac gaagcaaaag acaatgacaa caaagcagaa tcaaaccaat aaagctaacc
     7321 atactcgtca aaatgaacgg actacaccaa agaaagaaac agcactttcc aatgtgaaaa
     7381 agcaacaagg cggtatcggg caacgattag gtgaaaagac agcccatgta ttggacacga
     7441 aaaatcgtct agtcaataaa agaaagcagg caaaacaaca gatacaggcg acaccaaccc
     7501 atgtcaaata tgcccttcat caaggaaaag agaatgtcac tcataatgta acagacttta
     7561 aacaatctat ggcagatacc aaagctcaac gcaaacaaca gcgtatgaac cagcaggagc
     7621 aaaaacgaaa gaacattgct caaaagcgtt tggagctgga taaagcaaaa gaaaaaagac
     7681 gaaaagaaaa cccatcatct cctgttcaga ataaccagca gcatgttcgc aagcgtcagc
     7741 aaaccgtcac gcctgttttg tcacaaaaca ctaaacgtga aaatcatcag cgaccattga
     7801 aaaaagagca aaaacaaaag acaccaccga agcaattcaa tcagaggaag ctaaataaaa
     7861 gaacggataa gcgaggacaa caaacatgaa gctaaaaata acagtgattt gtagtgtggt
     7921 cttctctctc ttcttttttc tcttattatt cctagtcatt ttctttgcag atgatacgga
     7981 cagtggcgaa aacaacaagg attcttctat tccacaaggc ggtgtgaccg tttcaccaga
     8041 agtgttggca caccgtccgt tgattgaaaa atatggtaag gaatatggga ttgaagacta
     8101 tgtttcttat atcttagcca ttatgcaagt ggaatcgggc ggtacagcag aagatgttat
     8161 gcaaagttcc gaatctttgg gtttaccgcc caacagtttg agtaccgaag agtccatcaa
     8221 acaaggggtt aaatatttca gtgaattatt aaccagtgca gaacaacaag gagtggatat
     8281 agattctgtt atccaatctt ataattatgg cggtggcttt ttaaattatg tgaggagtca
     8341 tgggaaaaaa tatacctatg aattagccga acagttttct aaagagaagt cgggtggtca
     8401 aaaagcagac tatcccaatc ctatagccat acctgtgaat ggtggttggc gatataacta
     8461 tggaaatcag ttttatgttc agttggtttc acaataccta accgacactt ccccaacaga
     8521 atttgatgat gaaaccgtgc aagtcattat ggacgaagcc ttgaaatacg aagggtttcc
     8581 ctatgtcttt ggaggggcgt cgcctaccac ctcttttgat tgtagtggct taatacaatg
     8641 ggtgtatgac aaagctggca tttctctccc tcgtgtcgcc caagatcagt atgacgctac
     8701 gcaagaaatc tctatggaag aagcccaagc aggcgacctt atcttctttc attcaaccta
     8761 taatgcaggc acatatgtga ctcatgtggc gatatattta gagggaaatc gcttttatca
     8821 tgcaggcgac ccaatcggtt atggcgactt aagcagtcgt tattggcaag accatctgat
     8881 tggggctaga cgtgttattc ataactaaga aaggaatgaa tcaaatgatt ataaaaatcg
     8941 agcgaaaaga aaagaaggag aaaccaaaga aatctaaaaa accgagcaag caaagaaaag
     9001 ttccgatgat taaagttggc acgcataaga aattaacctt tgttttatgg gttttattga
     9061 ttggtagtgt cggatttgga atctataaaa attttactgc cattgacact catactgtcc
     9121 gtgaaacaga aatcattaaa cagcagattg tcgataccaa ccaagtagaa agttttgtga
     9181 aatcctttgc gaaagactac ttttcgtggc aacaatccca agaagccatt gataaacgaa
     9241 atgaaaaatt gacccattat ttgacggaag aattgcaggt attaaatgaa gaaatgattc
     9301 gtaaggatat tcctactagt tctagtgtga acgatataca ggtgtggcag gtttctcaag
     9361 tgaatgagaa cacctttgag gtactgtttt ctgttgaaca agttattacc gaagacaaag
     9421 acaaggaaac tatatcgtct agctttcatg tggttgtcca tatagatgaa tcagataata
     9481 tggttattat caaaaatccg acaatgagta agaaacctca aaaatcggac taccaaccca
     9541 aacagctaga aagtgaccat acagtagata cagaaacgat ggacgaaatc actagctttt
     9601 tagaaacgtt cttccagctc tatcctaccg ccacagaaaa agaattgact tactatgtaa
     9661 gtaatcatgt gttacctatg attaacaagg aatatgtttt cgaggaattg gtgaatccta
     9721 tctttaccag aaaagataac caagtaatcg tgaatgtcgc tgtgaaatat ttagatcaag
     9781 aaacaaaggc aacccaaatc tcgcaatttg agttgatatt agaaaaacaa gataattgga
     9841 agattgtaaa ataacaaata ttggtacatg attacagata ctttgtaatc atgtactctt
     9901 tttgataaaa aattggagat tcctttacaa atatgctctt acgtgctatt atttaagtga
     9961 ctatttaaaa ggagttaata aatatgcggc aaggtattct taaataaact gtcaatttga
    10021 tagcgggaac aaataattag atgtcctttt ttaggagggc ttagtttttt gtacccagtt
    10081 taagaatacc tttatcatgt gattctaaag tatccagaga atatctgtat gctttgtata
    10141 cctatggtta tgcataaaaa tcccagtgat aaaagtattt atcactggga tttttatgcc
    10201 cttttgggtt tttgaatgga ggaaaatcac atgaaaatta ttaatattgg agttttagct
    10261 catgttgatg caggaaaaac taccttaaca gaaagcttat tatataacag tggagcgatt
    10321 acagaattag gaagcgtgga caaaggtaca acgaggacgg ataatacgct tttagaacgt
    10381 cagagaggaa ttacaattca gacaggaata acctcttttc agtgggaaaa tacgaaggtg
    10441 aacatcatag acacgccagg acatatggat ttcttagcag aagtatatcg ttcattatca
    10501 gttttagatg gggcaattct actgatttct gcaaaagatg gcgtacaagc acaaactcgt
    10561 atattatttc atgcacttag gaaaatgggg attcccacaa tcttttttat caataagatt
    10621 gaccaaaatg gaattgattt atcaacggtt tatcaggata ttaaagagaa actttctgcc
    10681 gaaattgtaa tcaaacagaa ggtagaactg tatcctaata tgtgtgtgac gaactttacc
    10741 gaatctgaac aatgggatac ggtaatagag ggaaacgatg accttttaga gaaatatatg
    10801 tccggtaaat cattagaagc attggaactc gaacaagagg aaagcataag atttcagaat
    10861 tgttctctgt tccctcttta tcatggaagt gcaaaaagta atatagggat tgataacctt
    10921 atagaagtta ttactaataa attttattca tcaacacatc gaggtccgtc tgaactttgc
    10981 ggaaatgttt tcaaaattga atatacaaaa aaaagacaac gtcttgcata ttaagtagtt
    11041 cagttttgga gtacaaaaag tgaaaatgta aacctaaatg agtatcaaat tgcaactgaa
    11101 tttgatactc gttttttatt gttaaatcaa cgtttgttgt taatttacac cacaaaatgt
    11161 aagttaaaat gtatataaaa atgcgaacgt gaaataatta ttgtaggaat gcaaattggt
    11221 gtgttaaaat ggttacaatt tactaagcaa aaaggggtgc attttcctat gcaatttctt
    11281 gagccaaaaa ataagaacgc taagtctgtg gattgggaaa tatcagagca agtaagggtg
    11341 atagttaagc aatacgctga atatgcagag cgtactgaaa gcgaagcagt ggatgaattc
    11401 ttattgaaca ttttagacga taagaagttt attgaatgga ttgccaacaa gcgaagtaat
    11461 aagcggattg ttgaaaaaat gggtatcaaa gatagagtgg gttgatagac aatgaccaag
    11521 cttaaaagat tagccacgaa ggaagatgaa attgtagatg ttaaaattcc gatttcaaac
    11581 gaagaattaa aagatagggc taaacaatat gatttattaa ctccaaaacg atttgcaaca
    11641 aggtataaca aaatgttgtt cttgccaacg tcttttaaat ggaacggttc tgaatatccc
    11701 attcaatata attactgcat caatccgttt tgttgtaatt ttgggaaaga acagcacaaa
    11761 ttcaaagatg taaaagggaa accaagtcga tataaaatga ctggtagttc caaagataaa
    11821 ggtcataaag gtatgtactg caatgacaat cctattggca gaggtgtatc gcaaaactgt
    11881 acagtaaccc ctttatctaa ttggtctgtt gttgaagaaa ttaaacgcct tatagaaatc
    11941 aatagcatcc aagatgttga gccagattat caatttcata aagaaggctg ctcggaagaa
    12001 gaatcaaccc cattcaatga gccgaagcaa ttttacaaaa gggggaaaag taggggcaaa
    12061 tctcaacgct atcaatgtaa ggcgtgtaag aagttcacta atgttctacc aaaaagagaa
    12121 gaaaccacta cttatcacca acaaaagaac accattcttc caatgttcgc taagatggtt
    12181 gttggaaggg tttcagtcag tcgtacttgt gatattttag gaattggagt tggaacctac
    12241 tatcataagc tggagtggct gtataggcgt tgtttggagt ttttagaacg atatgagaca
    12301 caaccccttc aaacaaagaa atttaacgag atgtggctta atacggacaa gatgcactac
    12361 tacctaaata acgtccgtaa gaaaggacaa ggttcaaaga aatatacggg gtttgaagac
    12421 cttaatatgc aaacgtatat agttgtatct gctgaggtct tatcaagata tgtgttccgt
    12481 tctgatgttg cctatgattg gaatatttca atggatgaat taaatgaaga tacaagaaaa
    12541 ttcaaagaag accatctcaa tacttttagt cgtaaaaatg ataggttaga ttggtcttat
    12601 tatccacaag aaccatccgc taacgatagc gaaaatagga atgcttatct tcacgaatta
    12661 gggaaaatta ctaataggag tagatttgtc gatggtttaa atgttgatgc tccttatact
    12721 acaactgctc actattggtt aattaaacaa atggttaatg ccgacgaatg gcgaatgatt
    12781 agtgatgatg acttctctat tagaaacgcc ttttatagag tattcacgaa agaattaagg
    12841 ttgagcgatg cacatcattt tatttgccaa gttaacaaga cgaaaagcag aaaacaatgc
    12901 ttaaaagaat tcggacaagc aaaagcagaa ctgctcgatt ggggagatat cagaggattc
    12961 aaaacaaagt ttctacggac tttggcatct cattatctaa ctgaattact aacaagtcat
    13021 caattccacg aagaggctat tagtaaagat ggagaaagat atcgcaaata tgccgataac
    13081 cctatcaaac atccattagc cactaaggat aaaggatttt attcagttga ttgtagaacg
    13141 gatttatctg ctctggaacc aaacgaaatc gcaaagatgc tattaaatgt taatgaccat
    13201 tcgaccaata gctttattca acagataaga cgttatatat catctcttga aagaccatta
    13261 acaacagctc gtggtgataa gaagagctat atttacgcta acttcaatcc gaagtatgct
    13321 caatttgcga ttacaattct aagaacttac tacaactttt gtcgcccttt taaatcagcg
    13381 gataaaaagg ttttgacacc tgctcaaaga ttagggatta cagataaaca atttgattgg
    13441 aaagatataa tttatttcaa atgacaaaaa gagcttggag taatcccaag ctcttgtgct
    13501 ttatctttac tgttcttctt caattaacgc acccgttaac tcaataagtt ttttctttca
    13561 tatacatgaa aatagtagtt atacggattt ttatcattct ttatcccttt ttgggcaaat
    13621 acctcattcc attcctcata attcacttct ggaaaaaaag tatctccttc gaattcatga
    13681 tgtatttttg tgatgtacat tttctcaaca taagggaaaa acaaattata aatctgttct
    13741 cctccgaaaa taaaaatttc ttcttcgttt ttacataact caaaaacatc ttctattgaa
    13801 tgaacaattt cacaaccatt aaaggtaaac cccttatctc tcgtcagaat aatatttctt
    13861 ctgtcaggta aggctcttcc gattgattca aggttcttcc tacctaatat tatcggatgt
    13921 ccctttgtag tatttttaac atattcccag tccttgggaa tcctccaagg aatgtcattc
    13981 tctttgccaa tcactctatt cttatccatc gcagcaatca aagaaacttt catttataac
    14041 acagcctttt taactaattt accatctaat ttcttatttc tatcaaaaaa ccttttctca
    14101 ttatcctgcc cgttagttga ataagcaatt ttttctataa gttatattat cataatacca
    14161 caaaatcaca aattttcatt caattatcat tttcaattgc attttataaa tcattttgta
    14221 tgtaatttgg tatgtaagtt aaaatgaaaa aagtagtcca ttttgaagta cattttgtca
    14281 ttttgtactc caaaactgaa ctacttactt gcatatatac gcctttatag tggagtacta
    14341 catttacgag attcggttag agtatcagaa aaagaaaaaa taaaagttac agaaatgtat
    14401 acttcaataa atggtgaatt atgtaagatt gatagagctt attctggaga aattgttatt
    14461 ttgcaaaatg agtttttgaa gttaaatagt gttcttggag atacaaaact attgccacag
    14521 agaaaaaaga ttgaaaatcc gcaccctcta ctacaaacaa ctgttgaacc gagtaaacct
    14581 gaacagagag aaatgttgct tgatgccctt ttggaaatct cagatagtga tccgcttcta
    14641 cgatattacg tggattctac gacacatgaa attatacttt ctttcttagg gaaagtacaa
    14701 atggaagtga ttagtgcact gttgcaagaa aagtatcatg tggagataga actaaaagag
    14761 cctacagtca tttatatgga gagaccgtta aaaaatgcag aatataccat tcacatcgaa
    14821 gtgccgccaa atcctttctg ggcttccatt ggtttatctg tatcaccgct tccgttggga
    14881 agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc atttcaaaat
    14941 gcagttatgg aagggatacg ctatggttgc gaacaaggat tatatggttg gaatgtgacg
    15001 gattgtaaaa tctgttttaa gtacggttta tactatagcc ctgttagtac tccagcagat
    15061 tttcggatgc ttactcctat tgtactggag caagccttta gaaaagctgg aacagaattg
    15121 ttagagccat atcttagttt taaagtttat gcaccacagg aatatctttc acgggcatat
    15181 aacgatgctc ccaaatattg tgcaaatatc gtaaatactc aactgaaaaa taatgaggtc
    15241 attattattg gagaaattcc tgctcgatgt attcaagatt atcgcaatga tttaactttt
    15301 tttacaaatg ggcttagtgt ttgtttagca gagctaaaag gatatcaggt taccactggc
    15361 gaacctgttt gccagacccg tcgtctaaat agtcggatag ataaagtaag atatatgttc
    15421 aataaaataa cttagtgcgt tttatgttgt tatataaata tggtttctta ttaaataaga
    15481 tgaaatattc tttaatatag atttgaatta aagtggaaag gaggagattg ttattataaa
    15541 ctacaagtgg atattgtgtc ctatttgtgg aaataaaaca agactacgaa tacgagtgga
    15601 tactatactt aaaaatttcc ctttatactg ccccaaatgt aagaacgaaa ctttaattaa
    15661 tgttcaaaaa atgaatataa taacaatcaa agagccagac gccaagacgc agagccgata
    15721 atttgagaaa tgaaactctc atcttatcgg ctctttttgt ttatctgaat tttactgact
    15781 agccttcaat atttcttgct tcattgatac cttttgctag tgcttccatt aaggatagtt
    15841 ctttgtctgt aaagctatcc atgtattttt ctacctgtaa ccttcgagtg cttttaatca
    15901 aattatctgt aggtaagaaa aattcatcaa tagatacatg gagtaatgat acaagatcat
    15961 acagcacttg tgtacttggg tgttgtcctt tgttttctat atttgttaaa taacgtgggt
    16021 caatttcaat cattgttccc acttgttcac gagtgagacc ccgtttcatt cgagcctctt
    16081 taatcgctaa accaacggct ctaaaatcgt atttatcttc ttttttacgc atactaaatc
    16141 acctctatac attttactgt tcctatataa ccataaacag gtatagaaaa acatgttata
    16201 tagtttatga gttcctatag aacactgtgg aatttattaa tgcgatgaat ccaatttcaa
    16261 atagaaaggt cgctgatttt atttacctat acaactttat aaaatttagt catactccta
    16321 gaaagtagat aaaattggcg acttaatgga gggaaaagtc gccatgagaa agataatctg
    16381 tcatctagtt ctcttaatta agttcttatc aaaaaaactt atccacctgt actggataga
    16441 tatgaccatc aatgtggttt tgcagattac atagatagaa aaatcaaagc atatgaacag
    16501 agaaaacaat ctgtttatgt gcttttttgg tcacttaata aatatctttt aggatttctc
    16561 ttcatttatg caacaaatcc cccgttcgtg tcggggttta gtatgaaggg agataaagaa
    16621 gcacatgcca ctccctttca cgacagaaag ggggtgaaaa gcatgaaacc atcttctttt
    16681 caagaaacca tagaaaatca gtttgactat atctgcaaac aagttattga agatgaacga
    16741 aaagattact tcaaacatct agacagacta tcagcaaaag aagtctcgtt ttctgatata
    16801 gataactacc ttgtcaactc tttttctact gttgatacgt acgtcactga tttccaacta
    16861 tttaaattat ttggattaag aatcaatgtt gaaagtgatt tattaagtga agccctgcgt
    16921 catctttcag agaaaaaacg gaacattatt ttacttcatt actttatgga tatgagtgat
    16981 gtggagattg cggagttact aaatttgaat cgttcgactg tttatcgtca tcgaataagt
    17041 ggactagcca tgattaaaga attcatgaag gagtgtaaag aatgaaaaca gcttatccaa
    17101 gggttccctt ccccctcatt gtaaaggcga cagatggcga cgtggaagca attaatcaga
    17161 ttgttaaaca ttatcgaggg tatacctcaa aacgttcatt acgtcgcatg acagacgaat
    17221 atggaaattc tcatatggtt attgatgaaa ccctacgtgg cagaatggaa acgaaactca
    17281 tctctaagat tttatcgttc gaaattaaat agtcccattt tttcgtggaa gcgtgtcgaa
    17341 tcgtatccac gcttcccgat ttgggaggtt tgttattcca tcaaagcata ttgtgtgttc
    17401 agtaggtttt gataggctaa caagccaaac gttctttgac aaccaaataa aggcaatcga
    17461 atacgtttcg gtaagaaaag agctgatgaa ctaacacgcc atgacctaca aaattagtga
    17521 gcgattcatg ttagtaattc gaaagtaatc tttagcagga ttgctgccat gactttctta
    17581 tcgtgataat gatactcccg tatagccata gtccgagcgt tagaagcgtc acaggcaatg
    17641 ggtacggcta catgagaacc atgcagaagt gaaattcctg tgagctttgc tagagctgtt
    17701 cgattgctaa gatattcttc tcttgtcagt gtattattat aaaactattt gatataatta
    17761 taggtgaata ttggagtttt cctagcctat aattgggaaa ggagtaataa tgataacggt
    17821 cacaaaaaag gatttagttg agctaggata tggaacttct ttttcagcag acatcataaa
    17881 aaaagcaaaa gaattgatga ttacaaaagg gcatatctac tatcaatctc gaaagctaga
    17941 tagagtccct cgtgaagctg ttgaagaaat tctaggaatt cggttttcaa atgaaaacag
    18001 gtctgagtag tttttgcaca gtaaggagta aaaactatgg ttaaagaccc aataaaaaaa
    18061 gcaaagaatg gaacctatta ttttcgtgca aatctaggtt atgattctaa agggaaaaaa
    18121 attcaaaaat atcgtagtgg attcactact aaaaaagaag caagagaaga atattcgaaa
    18181 ctgctattga tgaaaccaga agaattaagt gagaataagg ataaaatgac gtttaaacat
    18241 tatatctttg aaattttcct tccatggtat aagacacagg taaagctcag aacatatgaa
    18301 aatcgcttac caactattaa gaagcatttc tcttattttg ataaaatggc tgtttccgat
    18361 attgagccta ttgatgtgca aaattggcaa ctaaaattat caaagaagtg taagtcttct
    18421 tatgtaagag ctgttcaagg actattttca gttgctatgg atcgagcaat tgtccttggg
    18481 ataaccacca caaatccatc taagattatt ggcaatgtga aaaagcaaaa gtctaaaatt
    18541 gaattttgga caaaagaaga atttgaaaaa gtaatctctc taatttacaa agaagattac
    18601 tatcaacact ttttatttat ctctctgtgg tttttattta tgactggaat gcgaattggt
    18661 gaagctactg ccattcaatg ggaagatatt gattttgact caggagtact aaccatcaac
    18721 aaaacactct attacaagaa tcaatcgaat tatagatttg ttgaaccaaa aacaaaggcg
    18781 agcgttcgtc atattgtatt agataaatgc acattaactt atttatccga atggaaggaa
    18841 gtacaacaaa atctaattca gacagacttt gtaatgagct ataacggtat tcctacccaa
    18901 aaacatacac tatcatatgc tattgaacgc tatgctaagc tggctggtat ccatcgaatc
    18961 agaatccatg ggttacggca ttctcatgct tccttgttaa tcagtatggg agaaaatcca
    19021 cttatcatta aagatcgtct aggacacgaa gatattgaga cgactttagg aacgtatgga
    19081 catctttatc ctaacagtaa tttcgaggta gctaacaaat taaatggagc catttccttt
    19141 aaagaagcaa aacaaaatta tgatacttca ccaaagaatc aatttactgt agactacttg
    19201 cgaaacaaag aaatgaaaaa agtgcaataa aagtgcagta aaaagataag aagagccaga
    19261 aacccttggt ataagaggat ttctagctct cctacaacta ttcccactcc actgttgctg
    19321 gtggtttaga agtgatgtca tacacgatac ggttgacgtg ggctacttcg tttacgatac
    19381 gtacagagat tttttgcaag acttcccatg ggatacgggc aaagtcagcg gtcattccgt
    19441 cgattgaagt tacagcacgg ataccgactg tgtagtcgta agtacgtccg tcacccataa
    19501 caccgacaga gcggatccct ggaagaacag tgaaatattg ccagatatca cggtcaagtc
    19561 cagcagctgc gatttcttca cgcaagatag catcagattc gcgtacgatt tctagcttgt
    19621 cttcagtgat ttcgccaaga acacgaatac caagaccagg accagggaat ggctgacgcc
    19681 aaacgattga atcaggcatg cctaactgag tccctaattc acggacttcg tctttgaata
    19741 atgtgtttaa tggttcgatc aattcaaatt gcatatcttc tggcagtcca cctacattat
    19801 ggtgagattt gatcgtctgt gctgtttctg taccagattc gataacgtca gtatacaacg
    19861 ttccttgtgc taggaaagag acgccttctt caccagcaag ttttgttgct tcgtcatcaa
    19921 aaacatatac gaattcattc ccaatgattt tacgtttttg ttctgggtca gatacacctt
    19981 tcaacttgtc taagaagcga tctcttgcat cgactttgat gatgttcaag ccgaattttc
    20041 cgcctagact ttccattact tgttccgctt cgcctttgcg tagcagaccg tggtcaacaa
    20101 agatagatgt caattggtca ccgatcgctt tttgtaaaag aaccccaacg acactagaat
    20161 caacgccacc tgaaagacca agcaagactt ttttgtcacc aacttgttca cggatttttt
    20221 ctacttgcat atcgataaag ttatccattg tccagttacc ttcacagtga caaacatcaa
    20281 aggcaaagtg gcgcagcaat tcgattccgt attcggaatg acgtacttct gcatggaatt
    20341 ggataccata aaaattacgt gttgtgtctt ggatagaagc aatcggacaa tctttgcttg
    20401 ttccgactgt ttcaaagcct tcaggaactt tagttaccaa gtcaccatgg ctcatccata
    20461 ctgtttgttg ttttggtgtt tctttaaaca agacagcttg atcgcttgtt acttctaaca
    20521 aggctttccc atattcgcgg tttttagcag gctctacgga accgccaagt ttgtacgtga
    20581 tcaattgcat accatagcaa atccctaata caggaatacc taaattgaag atctcaggat
    20641 cgatcccaaa tgcattctca tcatatacac tgtttggtcc gccagaaaga atgattccct
    20701 ttggattcat cgcttttact tcttctgcag taatgcggtg gctcaataat tcggaaaaga
    20761 ctccaagatc acgaatacgt cttgtgatca attggttata ctgactaccg tagtctaata
    20821 cgataatctt ttcaacgttc gtcatatcgg caacgtttgt caaatttatt acccctatcc
    20881 ctttaaattt tatctcaaaa tagtcaaact attttaaaat gctaatattt tcgcatgaaa
    20941 atcgaatcga tgatatgatt ttcagtttta tggagaatta tatcggctct tcctcgtgta
    21001 ggcaagatat attcctgtaa atttttcaaa ttcacatttt tccatacatc ttttgccatt
    21061 ttaaaggctt cttcacgatc tccgatagca aaacgataat aataattctc tggattttgg
    21121 aaagccgtat ccagtaacgc accaaaacga tccaaatacc atttttcgat cagcacggga
    21181 tcggcatcga catagacaga aaaatcaaaa aagtcgctga cgtagatttg ctgattcgca
    21241 ggtaattgaa gcgtattgat cccctcaacg atcaagatat caggctgctg gatcaattcg
    21301 tacttgtctg gaatgatatc atacacatca tgtgagtaaa caggtacttt tatctcttcc
    21361 tgaccagatt ttacacggtt caagaaatcg atcagcatct ccatatcata actttcagga
    21421 aatcctttac ggtccatgat tccacgctct tttaatatac gatttggata aagaaatccg
    21481 tcagtcgtga tcagctgaac gtttcttcgt ttgaaggtac gagacaaaat cgtctgcagc
    21541 agtctggctg ttgtactttt tccaacggct acacttccgg cgatcccgat aataaaaggc
    21601 ggtacttcta cgtatttatg aagaaataaa cctttactca agaacaaaga ttcaaattct
    21661 ttcatataaa gataaatcag atgagcaaga ggtacataga tatctcgaac atcctgcatg
    21721 gaaatgcggt cattcaaact tttgatatta tctagttcat cttgtgtcaa aggtgcttgc
    21781 ccattacgat aaaattctcg ccattcttct cttgccacac ggtagtagtt cattggatca
    21841 ttcatattag cctcctgcgt tctcccatca atggaactat tttaacacaa aataaacgca
    21901 aaccccacca cttaagagag attgccgatc tttttgtata atctttataa tagaaagaaa
    21961 cggagtctta ttcttgactt gcgcacggat acataaaagg atagactgtt gtcatgaaag
    22021 aaatgagaag gatggaagac atgacaaatc attactattc tgaacgccca gatacggcgc
    22081 acgattttga acaatgggct tttgagttaa aaggcagaac ctttcaattt gtaacagatt
    22141 ctggtgtatt ttcacgtgaa acagtggatt atggttcacg tgtcctgatt gatgctttcg
    22201 attgggaaga gttacctgat gaaggaaaga tcttagatgt gggatgcggg tatggaccga
    22261 ttgggttagc tttggcattt gcgacacagc ggtttgttga aatggtcgac attaatgaac
    22321 gagcggtaag tctagcgcaa ggaaacgcaa agcgtaacca aatcgaacat gttgatatcc
    22381 atcaatcgaa tatctatgaa gcagttcatg aagaaaccta tgcagctatc gtaagcaatc
    22441 cgccgatccg tgctggaaaa aaagttgtac atgaaatttt gacaggcgca tatcctcggt
    22501 tgaagaaggg cggtacactg acaattgtga tccagaagaa acaaggagcg ccgagtgcgc
    22561 aaaagaaaat ggaagaaaca tttggtaatg cagaaatcgt cacaaaagat aaaggatatt
    22621 atatcatcaa aagtgtgaaa gaataactag ctgaaaaata gtggagcgac gaaagagtgg
    22681 aactgggcgt gcatgtacgg gtacgtatca gttctttttt tgttgagaag tttttaaaaa
    22741 caaagaacta ttttttcttc aaaaagaaat agtaggaaca gagtcgttgt tttttacgat
    22801 aaaataaata agatacagaa cttgtataga aggctgccaa gtcggtagat aaaggaggtt
    22861 tttatcaaaa aacatacttt ccttttttct ctattctttt cttgggggtt ttggtacact
    22921 gatagggaat ttagatagaa gggagccaat aggatgaagc tgaccatcag agatatcgca
    22981 gaaatggcag gcgtatcagt aacaactgtc tcacagattt tgaataacaa aggaagcaga
    23041 tttagtgata agacaagaaa aaaagtgttg tcgatcgtga atgaacatca ttataagccc
    23101 gattatttcg cttcaaatgt gatcaatcga cattcaaaga ccatcggaat gatcgtccct
    23161 gatgtcactg atttcttctt ctccaaactg atcgaaggtg tagagagcta tttgaatcct
    23221 ttgggctatg tgatcatgtt atgcaattct cgtcatagcc aagaaaatga gatcaagtat
    23281 ttacaagagc tttcccaccg ctcagtggac ggaatcttgc tggccacacc aaatatttta
    23341 cctgagaaat atgcattaga cagcggtttt tatcaaaaaa tgccaatcat tttaatcgat
    23401 cgcggattga atcgtcggga taacggacgg cttatcgtaa aggaatatga gggcgcctat
    23461 caagctgttt cgctgtttat tgaaaatggc catacgaaga tcggcatgct gaaggaaacc
    23521 actggatact atcaattaga agaacgtttc aacggatatc gtcatgcgct gaaagatcat
    23581 ggcctgcctt ttaatgggaa atttgttgaa catggagagt tgactgtcca aggtggatat
    23641 actgcatcaa aaaaattact caaacataaa gatatcacgg ctattttttg cgggaatgat
    23701 gccatggcga tcggatgcta tcaggcgatc gatgaattgg gcaaaaaaat cccagaagat
    23761 atctcagtca taggattcga tggattaaag ctgtcagaat acatgattcc gcaattgacg
    23821 acagtgcagc agccaagttt tgatattgga ttttatgctg cacgtttcct gatcgatacg
    23881 atcgaatttc ctcaaagaaa agttccgaac aaagtgttcg aaacaaaatt aattattcgt
    23941 gagagcgtaa aagtattgac aaagggctaa ctcggcggta tattgcttat gtaagcattt
    24001 acaaaggcta gttagtgctt tttttatttt tagtaaacca gtttactaaa gtggtttacc
    24061 tttcctaaaa ataatgtata ataccgaatg aagacaagga ggatcaggca atgcgaatgg
    24121 tggatctgat tgaaaaaaag agggatggaa acgagctgtc gaaagaggaa atcgagtaca
    24181 tcgtcacgaa ctatacgaat ggaaagattc cagattatca agtcagtgca ttattgatgg
    24241 caatttttta tcaagacatg acaaatgaag aaatcacgaa cttaacattg gcgattgcta
    24301 attctggtga tgtcatcgat ttgagttctc ttgaaggaat aaaggtagat aagcattcga
    24361 caggtggcgt tggtgacacg accacattga ttttagcccc cctagtagct agtgtcggag
    24421 tgacagtggc taaaatgtct ggacgcggtc taggttacac aggtggtaca ctagacaagc
    24481 tagaagcaat tcccggattt cagatcgagc tttcggatga agaatttgta cgcattgtta
    24541 atgaaagtaa agtagcggta attggtcaat caggaaactt ggcaccggcg gacaaaaaat
    24601 tgtatgcctt gcgtgacgtc acagcgaccg ttgattctct tccgctgatt gccagttcga
    24661 ttatgagtaa aaaaatcgcg gcaggagcag atgcaatcgt tttggatgtg accactggag
    24721 acggtgcttt catgaagaac atcgaagatg caagacgtct ggcaaagacc atgacgagca
    24781 ttgggaaact ggctaaccga gaaacagtgg cggttatctc agatatgtca gaacctcttg
    24841 gcgaagctat cggaaatagt ctagaagttg tcgaggcaat cgaaacatta caaggaaatg
    24901 gtccagaaga tctagtagaa atgtgctacg ccttaggaag ccaaatggtt gttcttgctg
    24961 gcaaagctaa gacaaccgat gaagcaagaa cgttgcttca agaagctctg gaatcaggta
    25021 aagcgttagc aaaattcaaa gaaatgatcc aaaatcaagg gggagaccca gcgatcgtgg
    25081 aacatccaga acgaattttg actgcacgct acacaatgga gcttcctgct aaacaaagcg
    25141 gagttgtgtc aaaaatcgtg gcaaatgaat taggaattgc tgcaatgatg ctaggtgctg
    25201 ggcgtaaaac caaagaagaa gatattgatc acgctgttgg attgaaactg cataaaaaaa
    25261 ttggtgacac tgtgacaaaa ggtgagtcac tattgacgat ctatagtaac gacgaagaaa
    25321 tcacttctgt cattgaatta ttatataaaa atattgaaat tggtgaatcg gcaatgaagc
    25381 caacgttgat tcatgacatc atcacagaat aaaggaaaaa agcggacttt accgttttaa
    25441 agaaaagggg aaaagaaatg gaactaaacc gaatgatcga ccatactatt ttgaaagcag
    25501 acgcatctaa agaagctgtg atgcaaatta tcgaagaagc aaaaaaatac catttttatt
    25561 ctgtctgcat caatccagca tgggttgcgc tagcgaaaga acaattacaa ggtgagccag
    25621 tagccgtatg tacagtaatc gggtttcctc taggagcaaa cacacccgag acaaaagcat
    25681 ttgaaacaac agatgccatc aataacggtg cagatgaagt cgatatggtc atcaatatcg
    25741 gtgctttgaa atcaaaggat gatcaacaag tccaaaaaga tatcgaagca gttgttgaag
    25801 cagcaaaaga tcgtgcgctt gtcaaagtta ttattgaaac atcgttatta gatagagaag
    25861 aaatcatccg agcttgcgaa atagcaaaag ctgcaggcgc tgattttgtc aaaacatcaa
    25921 ctggtttctc aacaggcgga gcaaaggtag aggatgtccg tttgatgcgt gaaactgtag
    25981 gaccagaaat gggtgtcaaa gcttccggcg gtatccataa cgaagaagaa gcaatggcaa
    26041 tgatcgaagc tggtgctaca agaatcggaa caagcgcagg agtagcaatc gtatctggtt
    26101 caactggtga aggctattaa ttttttcaaa ggagaagctc atggcagaac tcaaacaaga
    26161 atggatcaac acagctattg aagcgttaga taaagcctat gttccttatt cccattttcc
    26221 ggtaggcgct tgtctcgtaa cggaatcagg caagatttat caaggcatca acattgagaa
    26281 tgcatcattt ggattaacga attgtgccga gcgcacagct tttttcaaag cagtatctga
    26341 aggagaaaaa acatttacgc atctagtcgt agcggggcac acccccgatc ctatctctcc
    26401 ttgtggtgca tgtcgacaag taatggcaga attctgttcc cctgatatgc cggttacgct
    26461 agtaggagac aatggtgtga cgaaggcaac gaccgttcgt gcactgctgc cttatgcatt
    26521 tactgaaaaa gatctataac tcatgttcat tcagtcagct gactgttgaa aaataaaaca
    26581 attattttag gaggctttac agaaatgaaa aaagcaaaat tatttggttt aggtgctgtc
    26641 gcactagcag cagggttatt tttaggtgca tgcggcaaca acggctctac tgattcatcc
    26701 ggtggcaaaa gctcatcaga cacaacaact gcagcattga tcacagacac aggtggtgta
    26761 gacgaccgtt cattcaacca gtcagcttgg gaaggactag aaaaatgggg gaaagatcaa
    26821 ggactttcac gaggaaatga tggcttccaa tatttccaat cttcaaatga atcagattac
    26881 atcccaaata ttgaccaagc attgaatgct ggattcaaaa cgatttttgg tattggttat
    26941 aaactaaaac cagcgatcca agaacaagca acaaacaata caggtacgaa ctttgttatt
    27001 atcgatgatg tcatcgatgg attagataac gttgtatctg caactttcaa agacaacgaa
    27061 gcttcttatc ttgcaggtgt tgcagcagct tatacgactg aaacaaacgt agtcggcttt
    27121 atcggcggtg taaaaggtga agtaatcgat cgttttgatg ctggattcaa agccggtgtt
    27181 gatgcaggtg cgaaagaatt aggaaaagaa atcaaagtat tgaatcaata tgctggtgac
    27241 ttctctgctc cagataaagg acgttcaatt gctcaaggta tgtatgcaca aaacgcagat
    27301 atcatcttcc atgcttcagg cggtacaggt aacggtgtat tccaagaagc aaaatcattg
    27361 aacgaatcag gcgacaaaaa agtttgggtt atcggtgttg accgcgacca atcagacgaa
    27421 ggcgaataca cattaaacgg cgaaaagaaa aacttcacat tgacatctac actgaaagca
    27481 gttggaacag tagtagaaga tttggcacaa aaatcagcgg acggtaaatt ccctggcggc
    27541 gaacacactg tttatggatt gaaagaagac ggtgttggtc taacagaagg acaactttct
    27601 gacgaagcga aaaaagcagt tgatgaagca aaagaaaaaa tcatttcagg tgacgtaaaa
    27661 gttccagaaa ctcctgaaga aaactaaaac catttctata gccttgttag acacatgttt
    27721 gacaaggcta tggtttgatt agaacaagaa ttgttcattt atccaagctg ccttgtgaca
    27781 gtttggataa gggaacaact ccctgaagta atgtttgttt caaaagaaga acgctcacta
    27841 agcgtgaatc ttagtcaatc gttgccctag atagaaatcg ttaggatgtg aaaaaaaggt
    27901 ggttcaagaa aatattgtca tagaaatgca gaatattacc aaagaatttg gaacattcaa
    27961 ggcaaatgac aacatcaatt tacaagtaaa ggcaggagaa attcatgcgt tgcttggaga
    28021 aaacggtgct ggcaaatcta cattgatgaa cgtgctttcc ggattattag agccgacatc
    28081 agggaaaatt ttgatgcgtg ggaaagaagt aaagatcaca agtccgacca aagccaacca
    28141 attagggatt gggatggtcc atcagcactt tatgcttgtt gatgccttta ctgtaacaga
    28201 aaacatcgtg ttgggaagcg agcctagtcg cgcagggatg cttgaccata aaaaagcacg
    28261 aaaagagatc caaaaagttt ctgaacagta tggattgtca gtcaacccag atgcttacgt
    28321 tcgtgatatt tcagttggaa tggaacaacg ggtagaaatt ttaaaaacac tttaccgagg
    28381 agcagatgta ctgatttttg atgagccgac agctgtattg acccctcaag aaattgatga
    28441 attaatcgtg atcatgaagg aattagtcaa agaaggcaag tcgatcattt tgattacgca
    28501 taagttagat gaaatcaaag cagtagctga ccgctgtaca gttatccgcc gtggaaaagg
    28561 aatcggtaca gtcaacgtta aagacgtaac ctcacaacaa ttagccgata tgatggttgg
    28621 aagaacggtt tcattcaaaa cgatgaaaaa agaagcgaag cctcaagaag tcgttttgtc
    28681 tattgaaaat ctagtggtga aagaaaatcg tggattagaa gccgtgaaaa acttgaactt
    28741 agaggttcgt gctggcgaag tacttggtat cgctggaatc gatggaaacg ggcagtcgga
    28801 gttgatccaa gctttgactg gtttgcgaaa ggcagaaagc ggacatatca agctaaaagg
    28861 ggaagacatc actaataaaa aaccccgaaa aatcactgaa catggtgtag gacacgtgcc
    28921 agaagaccgt cataaatacg ggttagtcct agatatgaca ttatctgaaa atatcgcact
    28981 acaaacctat catcaaaaac cttacagtaa aaacggcatg ctgaattatt cagtaatgaa
    29041 tgaacatgcc agagaattga tcgaagaata tgatgttcga acaacgaatg aacttgttcc
    29101 cgcaaaagct ttatcaggcg gaaatcagca aaaagcaatt atcgctcgtg aagtcgaccg
    29161 agatcctgat ctgttgattg ttgcaaatcc aactcgtgga ctggatgtag gtgcgatcga
    29221 atttattcat aaacgtctga tcgaacaaag ggacaaatac aaagcagtgt tactgatcag
    29281 tttcgaatta gaagaaattt taaatgtttc ggatcgtatt gctgttatcc atgaaggaga
    29341 aattgtcggt atcgttgatc cgaaagaaac atctgaaaat gaattaggtc tattgatggc
    29401 gggctatacc ttagaagaag cacgaaaaga gttagaaaag ggggctggcg cacatgaatg
    29461 atcgcagcga aaaattccgt aatttattag tgcctgtact ttcagttgtt ttaggattga
    29521 tcttaggcgc gatattgatg gctgcttttg gttttcagcc catccaaggt tattcttcta
    29581 tgctagatgc agcccttggt tcgcaaagag ggataggtga aacgttaagg caagcaacgc
    29641 cgttgatttt tacagcatta ggtttttctg tagctaactc agctgggttt ttcaatattg
    29701 ggctttctgg acaagcactt tgcgggtgga tcgtcagtgt ctgggtggca cttgcgtttc
    29761 cagatctgcc aaaagttgtt ttgcttccgt tatgcgtgat cttaggtgcc ttagctggtg
    29821 cgatggcagc ggctgttcca ggtttacttc gagcattttt tggaacaagt gaagtgatcg
    29881 taacaatcat gatgaactat atcttattat acgtaagcac atttgcttta catgaagtga
    29941 tgcctgaaag ctatcgctca agtttggact cttccaatat gatcagtcaa aatgcctcac
    30001 tgcggttacc atggttaaca caagcttttg gcggctctcg cgtaaactca ggattatttt
    30061 tagcaatcat cgcgcttatt ttcgtttggc tgctgatgaa gaaaacgact ttaggatttg
    30121 aaatccgttc tgttggattg aatccttttg cttctgagta tgcaggaatg agtagtaaac
    30181 gtacgatcgt tttgtcgatg gtcatttctg gcgctttggc agggcttggc ggtgtggttg
    30241 aaggattagg aacgtatcaa aacttctttg ttcaaacgac ttcgctatct attggatttg
    30301 acggaatggc tgtttccttg cttggttctg gatcagcaat cggtattctt ttatcagcga
    30361 tcttgttcag tattttgaag atcggtggac ttggcatgca gacaggagca ggtgttccgt
    30421 ttgagatcgt caatgtatcg attgctttga ttatcttctt tgtcgggatc aacttcctga
    30481 ttcgtttcgt catggctaaa gtcttccaag ggaaaaaaca agaagagatc gttgcgacaa
    30541 tagaatcgaa accaacgaat caaaaccatg aagggggcga actataatgt cgactatcga
    30601 attaattgcc ttgatcctta cttctacact ggtttattca acaccgctaa ttctaacgtc
    30661 actaggcgga actttttctg aacgaagcgg gatcgtaaac gtagggctag agggaatcat
    30721 ggtcatgggt gcttttagtt ccatcgtttt caacttgacg atgagtccat cacttggttc
    30781 tatgacacca tggatcgcta ttttagtggg tggcgtcgta ggtatcctat tttctctgat
    30841 tcacgcagta gcaacagtca gtttgcgtgc tgaccatatt atcagcggga cagtaattaa
    30901 tctgatggca cctgctttag gtgttttctt gatcaaagca tggtatggta aaggtcaaac
    30961 tgataacatc acgcagaatc tggggtattt ttctttccca ggattagcag atattccggt
    31021 cattggacca attttcttta aaaatacatt cttacctgct tattgtgcta ttttgattgc
    31081 tgtcctagct tgggtgatca tcttcaagac gaaattcggc ttgcgcctcc gttcagttgg
    31141 tgaaaatcct caagctgccg acacattagg tatcaatgta tatgctatgc gttattcagg
    31201 cgtactgatt tctggcttgt taggcggtat gggcggtgct gtttttgctc agtcgatttc
    31261 cggtaacttt tcagctggta cgatcgttgg acaagggttc atctccatgg ctgctatgat
    31321 ctttggtaag tggaatcctt taggggcaat gggtgcctca ttgttctttg gttttgctca
    31381 aagcttgagt atcattggcg cacaattacc agttatttct tcgattccgc cagtattgct
    31441 gcaaattgct ccatatttgc tgacaatcat tgtgctggtt gttttcttag ggaaagcttc
    31501 tggtccaaaa gcaaacggga agaactatat caaatcaaaa taatctttta gcgaataaaa
    31561 agggtgggtt acgggaagtg tttttcgtct cgcccttttt gttttaaagg caataaaaat
    31621 aaaa
//
LOCUS       DAZULO010000271        13213 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.guided.073, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000271 DAZULO010000000
VERSION     DAZULO010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.guided.073"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..2162)
                     /gene="nagE"
                     /locus_tag="ACG8OA_003024"
     CDS             complement(204..2162)
                     /gene="nagE"
                     /locus_tag="ACG8OA_003024"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFS4977732.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="ACG8OA_003025"
     CDS             complement(2159..3031)
                     /locus_tag="ACG8OA_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4977733.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="ACG8OA_003026"
     CDS             complement(3373..4245)
                     /locus_tag="ACG8OA_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4977734.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="ACG8OA_003027"
     CDS             4373..4942
                     /locus_tag="ACG8OA_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFS4977735.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="ACG8OA_003028"
     CDS             5009..5746
                     /locus_tag="ACG8OA_003028"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4977736.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="ACG8OA_003029"
     CDS             5977..7245
                     /locus_tag="ACG8OA_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS4977737.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="ACG8OA_003030"
     CDS             7732..8196
                     /locus_tag="ACG8OA_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4977738.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="ACG8OA_003031"
     CDS             8180..8809
                     /locus_tag="ACG8OA_003031"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS4977739.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="ACG8OA_003032"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="ACG8OA_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS4977740.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="ACG8OA_003033"
     CDS             10688..11134
                     /locus_tag="ACG8OA_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS4977741.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="ACG8OA_003034"
     CDS             complement(11333..11848)
                     /locus_tag="ACG8OA_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4977742.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="ACG8OA_003035"
     CDS             11965..13167
                     /locus_tag="ACG8OA_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4977743.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DAZULO010000272         8028 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.guided.110, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000272 DAZULO010000000
VERSION     DAZULO010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.guided.110"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..524
                     /locus_tag="ACG8OA_003036"
     CDS             <1..524
                     /locus_tag="ACG8OA_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS4977744.1"
                     /translation="MNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKRDVTTNYFIL
                     PHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQKKLVTDNQAI
                     AHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKRFITNDSTIY
                     EQYEYDLSSVENK"
     gene            complement(674..1615)
                     /locus_tag="ACG8OA_003037"
     CDS             complement(674..1615)
                     /locus_tag="ACG8OA_003037"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS4977745.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1741..2862
                     /locus_tag="ACG8OA_003038"
     CDS             1741..2862
                     /locus_tag="ACG8OA_003038"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS4977746.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3120..4622
                     /gene="eat(A)"
                     /locus_tag="ACG8OA_003039"
     CDS             3120..4622
                     /gene="eat(A)"
                     /locus_tag="ACG8OA_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS4977747.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4689..5216
                     /locus_tag="ACG8OA_003040"
     CDS             4689..5216
                     /locus_tag="ACG8OA_003040"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4977748.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5427..6488
                     /locus_tag="ACG8OA_003041"
     CDS             5427..6488
                     /locus_tag="ACG8OA_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFS4977749.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6491..7387
                     /gene="murQ"
                     /locus_tag="ACG8OA_003042"
     CDS             6491..7387
                     /gene="murQ"
                     /locus_tag="ACG8OA_003042"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS4977750.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7401..7988
                     /locus_tag="ACG8OA_003043"
                     /pseudo
     CDS             7401..7988
                     /locus_tag="ACG8OA_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
BASE COUNT         2697 a         1405 c         1738 g         2188 t
ORIGIN      
        1 atatgaattt ttcttctagc gaaccaaaga cggatctatt ttttggtgca acgaataatg
       61 ggagaaaaga acgagcagaa agctataagc aggaatttat aaaaagagat gttacgacca
      121 actatttcat tcttccacat cgagggaatg aacaacctgg atatcttact tatgaagagt
      181 acctgaaaaa aacaggggaa tcgcgcggga ttctggaact gttgcgggag ggtcagcaag
      241 gagtgacttt gcggacattt gaatccatgt attttcaaaa gaaactagtg acggataatc
      301 aagcgattgc tcattatcat ttttatcgtc ctgaaaatat ttttttgctt cgagaacgtg
      361 atttagatga attaccggaa tttctgcata caccatttaa gccaatccta tcagagctat
      421 tggaatttta tgatgcagaa aattgggcaa aacgatttat cactaacgat tccactattt
      481 atgagcaata cgaatatgac ctctcctctg ttgaaaacaa ataaaaaacc tacattttga
      541 agagattatg tactgaaaca aacaagcaca attcttatgg catcatgaga gttgtgtttt
      601 ctttttgatc ttttattggg cttaaaaaaa gagaaccagc tgctgattca gctgattctc
      661 atcagagagc cttttattct gcgccaagga ttccttcttt tgcatggact aattgggtca
      721 aaaaggcaca atatggtgta gcaatatcat atttttgtcc ttttcgccaa atggcaccat
      781 tgatgtagtc gatttcagtc aaacgatggt ttttgatcag gtcttggtac atagatgggt
      841 aatgcaaccc gatgttgtca ggatcataag tagaagcaat atgttggtac acttcttctt
      901 gatctagtat gattccttct tttgctgcga tagaagcaaa ttcagatacg attgttcgaa
      961 ccatgctctc tgccgctttt tgcgcgccta gttcagcaat attacaatcc aaaatcgtgc
     1021 ataaaccatt caatgttcca tttacacaag cttttcgcca aatggaataa cgaacgttat
     1081 ggctatagat cggatttaaa tttgcttctt gaaagacttc tacaactttt tttgtaaatg
     1141 cttcgccttc tggttccaag ttctctagtt cgatttctcc atcacctaac aatgtgactt
     1201 ttccgggacc agctagacca gctgtccaca tcgtgatccc aaataagata tttttctttg
     1261 gcacatattt ttccagtaca tcttcatgac ctaatccatt tagtaaacac aaaacataag
     1321 tattgtcagt aataatggat tggatcgaac aaaacatatc atctaattga ttggcttttg
     1381 ttaatgctac gatcaagtcc acgtgttcgt tgctttcgat gatttcttct ggtgaataga
     1441 ttggcagttt tgcgaccact tctttaccgt taaagtcagc aatcaaccca tttgtacgaa
     1501 tggcttcaat gtgagcaggc caacgatcga tcaagaggac ctcatttccg ctttgatgta
     1561 gcatcaatcc gattcggctg cccattgctc cggccccagc tattgcgatt ttcatgtttt
     1621 ttctctcctt tattcaatga gttccctttt agtatataga aaaatggaag aaaatagcat
     1681 tgttttgttt gaaaaaaatg aaaaaaagtg tcttacttaa gaagaaagtg agggatacag
     1741 atggatattt tggcggcaat tgattcattt aaaggaagtg cttcttcatt tgaactaaat
     1801 caggcagttc taaatggctg ggaagatcgt aaaggaagaa aaatcaatgt gccgattgca
     1861 gatggcggag aaaatactgc agaagcaatc tattacgcgc tcaggggaac atggcgtacc
     1921 attgatggga tggatctctt attccgtcag cgaaaaatca gctatttatt gacaacttgg
     1981 caaggaaaaa acaccgcagt catcgaatct gcacaagcat tggggttaga tctacttgat
     2041 aaaccaacgg atcaaactat ccgacttgct tccagttatg ccttaggaga acttattaaa
     2101 gatgcactgc ttcaagaagt agaacaaatt attgtcactt taggaggaag cgggtgtagc
     2161 gatggtggtc taggattact ccaatccttg ggtgcttctt tagaagggta cacagaagga
     2221 aatcctttat tatctactaa aaaacttcat tttgatcgaa gaaacgttct tgtttctaaa
     2281 gtaaaattga ttatcgctgc agatgttacc aatccatata caggtcaaca aggagctgct
     2341 tggcaatttg gaagacaaaa aggaggaata gatacgacgc tagctttctt agataaacaa
     2401 gcaggaaaga ttgctcagca aataaaagat ttctgggagg tggatttaga tgatattcca
     2461 ggaagtggag cagcaggcgg tattggtgga gccctttttt tgttgggagc gaagatggtt
     2521 tctggtttcg atttagtcag ccaaatcact ggattggaaa aaaagatcga acaagcagat
     2581 gtcatcatta caggagaagg gcggatcgat cagcaaacga tccacggaaa agttccatac
     2641 ggtgtagcag tgcttgcaaa aaaatatcga aaaccaataa tcgctatttg cggaagtaga
     2701 gaccaagagc tagaaggact ttctgatttt ctcccaattg tcttaagtat tcatttgggt
     2761 ccaataggtt tagaagaagc aatggaacac aggcaaacat tagaaaaaac aaaaatctta
     2821 ggacagacac ttagcagatt tttcaccatc ctcccgaagt agttgacaaa atcattttct
     2881 ttatgctatc gtaatttcaa atataggaca aagtgaggga tccgtgcatt gaaaaattaa
     2941 gtcgattgat gaaagctgca ttcagactgt accttgtgga gacaaggaag cagtgtgcag
     3001 attttcggga ttggcttggt tttttcgatg tatgggtctt tttgtgtgta tccaaccctt
     3061 gcatcctctt tagccagttc tgaaaatagg actggtcttt ttttacgagg tgatggaaaa
     3121 tgtctaaaat cgaaataaaa aatctgacat tcggctacga cagccaaggc acattattat
     3181 ttgaacaagc aaatctaaat tttgacacac aatggaaact aggacttatc ggacgaaacg
     3241 gtcgaggaaa gacaacttta ctgaatattc tacaaaacaa actaccttat caagggcaag
     3301 taatccatca gcaagaattt gcctatttcc cgcaacagac aaaagataaa gaacgtttaa
     3361 cctattacgt gttaaatgat attacggatt ttgagatatg ggaaatcgaa agagagctcc
     3421 aattgatgca aacagatcct gaaatcttat ggagagaatt cagcacacta tcggggggag
     3481 agaagacaaa agtcctactg gcacttttat ttgtggatga cactcatttc ccgttaatcg
     3541 atgaaccaac gaatcatttg gatatctctg gtagaaaaca agtagcggct tatttgaaaa
     3601 agaaaaaaca aggcttcatc gtggtcagcc atgaccgggg atttatcgat gaagtagtgg
     3661 accatgtttt agcaatcgaa aaaagtcaac tggaacttta tcaagggaat ttctctatct
     3721 atgaagaaca gaaaaaactt cgtgatgaat ttgaaatggc tcaaaatgaa aaattgaaaa
     3781 aagaagtcag taggctaaag aaaacagcag ctgaaaaagc cgaatggtct cgttcccgag
     3841 aaggagataa aacaaagaaa caagtcggat tcatcgatac tgaatctaga cgagtgaata
     3901 aaggagcagt gggtgctgat gctgcacgga cgatgaaacg atccaaagca atcgtgaatc
     3961 ggatggagac ccagatcagc gagaaagaaa aactattaaa agatatcgaa tatatcgatt
     4021 ctttgacgat gaatagccaa gcgtctcacc ataagcgact tttaagcgta gaagatcttc
     4081 aattagggta tgaaaatctg ttattcgagc caattcattt tacaatcgag cctcatcagc
     4141 gggtggcgat ttcaggtcct aacggtgcag gaaagtcatc cattatccat tatcttctgg
     4201 gggcattcaa cggcaaggtt ataggagaaa aataccagcc aaaacatctg agcattagtt
     4261 atgcaagcca aaattatgaa gacaatcgag gaacgttggc ggaatttgca gagaaaaacc
     4321 aagtagacta ccaagcattt ttgaacaacc tccgaaagct tgggatggaa agagatgttt
     4381 ttcataacaa gatcgagcag atgagtatgg gccaacggaa aaaagtggaa ttggctaaat
     4441 ctttatcaca gccagctgaa ctatatacat gggatgaacc attgaattat ttggatgtct
     4501 tcaatcaaga acaattagaa caactgatct tgaacgtgaa acctgccatg ttactagtgg
     4561 aacatgatca aaccttcctg gataaagtat ctactgagat tatttctctt gagagaatct
     4621 aacccttgta atctcaagaa caacaaggta tggttaaggg gaactttgcc aacgaaggag
     4681 agaatcacat gctttatgta acgagacacg gagaaacaac ttggaacgca caaggattag
     4741 tttgcggacg ggcagatgtg ccgctgactg aaaaaggaca gatgcaggca cagaaactag
     4801 cagaaaaggt agtagatttg cctgttccaa tcacgaaaat catccattcc cctcttcaac
     4861 gagcaagaga tacagcacaa gcagttgctg atcgtctcag cttgccgctg accgttgatg
     4921 aacgattggt agaaatggat tttggggatt atgatggact gccaagcaaa gatgagaact
     4981 ttcaaaaagc acgtttagcg ttcgctgtac gattcccaaa tggggagtca gtcttagatg
     5041 tgtatgcacg gatcgttcct ttattgaaag aatgcatcga agacgaagaa aatgtctatc
     5101 tgttagtttg ccataatgcc ttgataagag tgatcaatgc ttattttcat ccgatgccta
     5161 atgaggggtt ctttacattc atggtagata atacagaatt ggtttcttat gagtaattat
     5221 gtttttgtgt tatttaaata aaatatttca aggcactgct tttgcttagc agtgcctttt
     5281 ttaggttatg acaattcttt tgataatata aactcaacct ttagaaatga aatttaatta
     5341 ttttaataaa agtattgaaa ttttgtttct ttagatatat actctagtca tagaaatgaa
     5401 gacggataca gaaaaagagg gataatatgt ttggcttttc aatatttatg aacgattctt
     5461 tgtcagaaga gaagaaacag tatatccgcc aaatggcatc caatggcttt gtcggtattt
     5521 tcacttccat gcatatacca gaagatgatg tttcggcata tcaaaagcga ttgatcgaac
     5581 taggaagcga agcgaaaaag aataaactag aattgatggt cgatatttca ggagaggcat
     5641 tggaacgagc gggtttttca ttagaagatc taacgccttt gaaagcaatt ggtgtaacag
     5701 gtttaagaat ggattatcat atttccaatc agcagattgc cgactggtca catcaattga
     5761 aaatcagttt gaatgctagc acgatcacgc ctaaagatat cgatgaattg aaagaagcag
     5821 aagctgattt ctcacagatt gaagcttggc ataattatta tccacgtccc gaaacagggc
     5881 tggacaaaga atggtatcag aaaaaaaatc aatggctcaa aattcaaggt ctccttgtcc
     5941 aaggttttgt accaggagat acagagttga gaggcccact atatcaagga ttgccaactt
     6001 tggaagaaca tcgagggatc catcctcttg caggcgctct agatctatta gcaagtaaca
     6061 cggatattgt ttacatcgga gatgcaggtc tgtcagaaaa cgttcaggag caatttgctt
     6121 ctttccaaaa agaacagaca gtcttgctcc atacggaacc tgtagatgaa gaattttacg
     6181 aatatatatt agggaagcat acgaatcggc aagacgatgc aagagatgtc atacgaagcg
     6241 tagatgcacg ttttcgagag attccgccta tcccagcaag aaatacagct acgcgtatga
     6301 aagggtctat tactttagat aacgaaaagt atttgcgcta tatgggtgag atccaattaa
     6361 ccaaatatga tttgccagcg gatgaaaaag tgaatgtagt agctaaagtt atcaaagaag
     6421 agctaccatt gatcaaccaa atcaaagctg gtatgaacta tcaatttata agaaaagaag
     6481 gaagataaaa atgagcaaaa tcaatttaga gaacctaaca acggaacgca gaaaccaaga
     6541 aacttttgga ttagatgaga tgagtatttc tcaagccgtt caactaatga acaaagaaga
     6601 tcagaaagtg gccgctgcgg ttgaaaaaga actgacaagt atcgaaccag tgattgctaa
     6661 cacgattgag tcctttaaaa aaggtggacg tctgatttat atgggagccg gaacaagtgg
     6721 aagactaggt gtgttagatg ctgctgaatg cgtaccgaca ttcggtgtag aaccggaaat
     6781 ggtcataggt ctaattgctg gtggagaaga agcaatgact gtagctgtcg aaggggccga
     6841 agattcttat gagctagggc aagaagatct aatcaagctg aatttgacaa aaaatgatat
     6901 agttattggt atcgcagcta gcggtcgtac accatatgtg atcggtggat tagattacgc
     6961 aaaatcgatc ggtgctgtca caggaacgat ttcttgcaat aaacaagccg aaatcagtaa
     7021 acacgcggat ttcccaatcg aagtcgatgc aggtccagaa tttttgacag gctctactcg
     7081 attgaagtca ggaacagcac aaaaacttat cttgaatatg atttctacta tcagtatgat
     7141 tggtataggt aaagtattca ataacttgat ggtcgatgtc aaaccaacaa atgaaaaact
     7201 aatcgaacgc tcaaaacgaa tcatcatgca agcgacagaa gctgattacg aaacagctga
     7261 aaagtacttt gaagaagcag atcaaaatgt taagctggct atcgtcatga ttttgacaaa
     7321 tagtgataaa gaggaagctc aagcaaaatt agctgaagca aacggatttg taaaagggac
     7381 cttatgaggg ggaaaagacc atggcaaaaa acgaactgac tttggaagaa ctggcaaaac
     7441 aaatttataa aggggcaggc ggcatgggca atgtagaaag cattgtccac tgcatgactc
     7501 gtgtgcgtat gaagatcatc aatgatgatc ttgtaaatgt ctctgaatta aaagcgattc
     7561 caggagtgct tggtgtagta gaagatgaac agcttcaagt catcatcgga cctgggaaag
     7621 tgaacaaagt agcacagcaa atggttgatt tggcaggcgt gaaattagga gaaaaacttt
     7681 cttctgtaag tacagctaca gcaaattcat cagcatctgg aaaagaccgt gtaaacgaac
     7741 gggcacaaga aatgaaagcg gcacaaaaat cgaaacaaaa accatctaaa atcaaatcgg
     7801 tattaaaaga catttctaat atctttgtgc caatgattcc tgcatttgtc ggtactggga
     7861 tcgtggcagg gattgccgca gttttagcaa acttagtcac agccggaaca cttgatgcag
     7921 caacatggca acaatatatc gatgtgatga acatcttgaa aaacgggatg tttagctacc
     7981 tagttatcta ggctctataa tttatgggac tgatgaatca gaattgat
//
LOCUS       DAZULO010000273         5610 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.guided.132, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000273 DAZULO010000000
VERSION     DAZULO010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.guided.132"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..1023)
                     /locus_tag="ACG8OA_003044"
                     /pseudo
     CDS             complement(37..1023)
                     /locus_tag="ACG8OA_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1175..1870
                     /gene="vanR-A"
                     /locus_tag="ACG8OA_003045"
     CDS             1175..1870
                     /gene="vanR-A"
                     /locus_tag="ACG8OA_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFS4977751.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1848..3002
                     /locus_tag="ACG8OA_003046"
     CDS             1848..3002
                     /locus_tag="ACG8OA_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFS4977752.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3217..4185
                     /locus_tag="ACG8OA_003047"
     CDS             3217..4185
                     /locus_tag="ACG8OA_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFS4977753.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4178..5209
                     /gene="vanA"
                     /locus_tag="ACG8OA_003048"
     CDS             4178..5209
                     /gene="vanA"
                     /locus_tag="ACG8OA_003048"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFS4977754.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5215..5580
                     /locus_tag="ACG8OA_003049"
                     /pseudo
     CDS             5215..5580
                     /locus_tag="ACG8OA_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
BASE COUNT         1696 a         1091 c         1260 g         1563 t
ORIGIN      
        1 ttttattctt tatttaaaac tttgcaacag aaccctattt ccgataaccg tgtattgaaa
       61 ggcatcttcg aagccattat ccaatatttc gcaggctttt gtatatttag gctggtcgat
      121 atattcaccg actaaggcat tcttagctgt tcgagcgagt tcaatatccg taaacttaaa
      181 gatcgctttt actgcttctc taaacggttt tgaattcttt tttggaatgg aactgaagat
      241 gtttcttaaa aaatggacct ggcatctctg ccaacttgcg ttggtaaatg acttacgaat
      301 cgcagacact aggcctttat gggcatcaga aatgatgagt tctgtcccct ttaggccgcg
      361 ttcttttaag tattcaaaga agatggacca tgtgtcatca ctttcttcat tttgaatcat
      421 gaagccaatg atttcacggt cgccaccttc tgttatcccg atcgcaatat ggcagctttt
      481 agaaagcact cgatggtcct cacggacttt tatgtagaga acatcagtca tcagataagg
      541 ataattcgta cctgagagtg aacggttctg ccattcgttg accatcgggt ctaactgctc
      601 agtcaggcta gaaacaaaag atttcgatac agattttcca catagctctt caacaatctt
      661 tgaaacttta cgtgtcgaaa cgcccgaaac atacatctca agcattgaag cgagcagtgc
      721 cttttcattt cgctgataac gctcaaacac cgtcggtgaa aattcaccat cacgtgttct
      781 aggcactttt aattcgagtg tacccacacg agtcgtaaag tctcgctcat aatagccatt
      841 tctttgactc tgacgacttt ctgaacgttc atagtcatcg gcttgaatat attctgttcg
      901 ttgattttcc atcaattggt tgaaaacagt ggttaaaata tttttagaaa catcatcttt
      961 taccgaatgt tcaataatac tttgaacctc ttcgttgttc agtgtaaaat gtacttgggt
     1021 catgtaacgt cctcctgggt atgtttttgt ggttaaaaac attgtaccgt aaaagggctg
     1081 ttacatggcc ttttatcttt tacacaatta tatggacttt atcctataat ctgataaatt
     1141 attaagggca aacttatgtg aaagggtgat aactatgagc gataaaatac ttattgtgga
     1201 tgatgaacat gaaattgccg atttggttga attatactta aaaaacgaga attatacggt
     1261 tttcaaatac tataccgcca aagaagcatt ggaatgtata gacaagtctg agattgacct
     1321 tgccatattg gacatcatgc ttcccggcac aagcggcctt actatctgtc aaaaaataag
     1381 ggacaagcac acctatccga ttatcatgct gaccgggaaa gatacagagg tagataaaat
     1441 tacagggtta acaatcggcg cggatgatta tataacgaag ccctttcgcc cactggagtt
     1501 aattgctcgg gtaaaggccc agttgcgccg atacaaaaaa ttcagtggag taaaggagca
     1561 gaacgaaaat gttatcgtcc actccggcct tgtcattaat gttaacaccc atgagtgtta
     1621 tctgaacgag aagcagttat cccttactcc caccgagttt tcaatactgc gaatcctctg
     1681 tgaaaacaag gggaatgtgg ttagctccga gctgctattt catgagatat ggggcgacga
     1741 atatttcagc aagagcaaca acaccatcac cgtgcatatc cggcatttgc gcgaaaaaat
     1801 gaacgacacc attgataatc cgaaatatat aaaaacggta tggggggttg gttataaaat
     1861 tgaaaaataa aaaaaacgac tattccaaac tagaacgaaa actttacatg tatatcgttg
     1921 caattgttgt ggtagcaatt gtattcgtgt tgtatattcg ttcaatgatc cgagggaaac
     1981 ttggggattg gatcttaagt attttggaaa acaaatatga cttaaatcac ctggacgcga
     2041 tgaaattata tcaatattcc atacggaaca atatagatat ctttatttat gtggcgattg
     2101 tcattagtat tcttattcta tgtcgcgtca tgctttcaaa attcgcaaaa tactttgacg
     2161 agataaatac cggcattgat gtacttattc agaacgaaga taaacaaatt gagctttctg
     2221 cggaaatgga tgttatggaa caaaagctca acacattaaa acggactctg gaaaagcgag
     2281 agcaggatgc aaagctggcc gaacaaagaa aaaatgacgt tgttatgtac ttggcgcacg
     2341 atattaaaac gccccttaca tccattatcg gttatttgag cctgcttgac gaggctccag
     2401 acatgccggt agatcaaaag gcaaagtatg tgcatatcac gttggacaaa gcgtatcgac
     2461 tcgaacagct aatcgacgag ttttttgaga ttacacggta taacctacaa acgataacgc
     2521 taacaaaaac gcacatagac ctatactata tgctggtgca gatgaccgat gaattttatc
     2581 ctcagctttc cgcacatgga aaacaggcgg ttattcacgc ccccgaggat ctgaccgtgt
     2641 ccggcgaccc tgataaactc gcgagagtct ttaacaacat tttgaaaaac gccgctgcat
     2701 acagtgagga taacagcatc attgacatta ccgcgggcct ctccggggat gtggtgtcaa
     2761 tcgaattcaa gaacactgga agcatcccaa aagataagct agctgccata tttgaaaagt
     2821 tctataggct ggacaatgct cgttcttccg atacgggtgg cgcgggactt ggattggcga
     2881 ttgcaaaaga aattattgtt cagcatggag ggcagattta cgcggaaagc aatgataact
     2941 atacgacgtt tagggtagag cttccagcga tgccagactt ggttgataaa aggaggtcct
     3001 aagagatgta tataattttt taggaaaatc tcaaggttat ctttactttt tcttaggaaa
     3061 ttaacaattt aatattaaga aacggctcgt tcttacacgg tagacttaat accgtaagaa
     3121 cgagccgttt tcgttcttca gagaaagatt tgacaagatt accattggca tccccgtttt
     3181 atttggtgcc tttcacagaa agggttggtc ttaattatga ataacatcgg cattactgtt
     3241 tatggatgtg agcaggatga ggcagatgca ttccatgctc tttcgcctcg ctttggcgtt
     3301 atggcaacga taattaacgc caacgtgtcg gaatccaacg ccaaatccgc gcctttcaat
     3361 caatgtatca gtgtgggaca taaatcagag atttccgcct ctattcttct tgcgctgaag
     3421 agagccggtg tgaaatatat ttctacccga agcatcggct gcaatcatat agatacaact
     3481 gctgctaaga gaatgggcat cactgtcgac aatgtggcgt actcgccgga tagcgttgcc
     3541 gattatacta tgatgctaat tcttatggca gtacgcaacg taaaatcgat tgtgcgctct
     3601 gtggaaaaac atgatttcag gttggacagc gaccgtggca aggtactcag cgacatgaca
     3661 gttggtgtgg tgggaacggg ccagataggc aaagcggtta ttgagcggct gcgaggattt
     3721 ggatgtaaag tgttggctta tagtcgcagc cgaagtatag aggtaaacta tgtaccgttt
     3781 gatgagttgc tgcaaaatag cgatatcgtt acgcttcatg tgccgctcaa tacggatacg
     3841 cactatatta tcagccacga acaaatacag agaatgaagc aaggagcatt tcttatcaat
     3901 actgggcgcg gtccacttgt agatacctat gagttggtta aagcattaga aaacgggaaa
     3961 ctgggcggtg ccgcattgga tgtattggaa ggagaggaag agtttttcta ctctgattgc
     4021 acccaaaaac caattgataa tcaattttta cttaaacttc aaagaatgcc taacgtgata
     4081 atcacaccgc atacggccta ttataccgag caagcgttgc gtgataccgt tgaaaaaacc
     4141 attaaaaact gtttggattt tgaaaggaga caggagcatg aatagaataa aagttgcaat
     4201 actgtttggg ggttgctcag aggagcatga cgtatcggta aaatctgcaa tagagatagc
     4261 cgctaacatt aataaagaaa aatacgagcc gttatacatt ggaattacga aatctggtgt
     4321 atggaaaatg tgcgaaaaac cttgcgcgga atgggaaaac gacaattgct attcagctgt
     4381 actctcgccg gataaaaaaa tgcacggatt acttgttaaa aagaaccatg aatatgaaat
     4441 caaccatgtt gatgtagcat tttcagcttt gcatggcaag tcaggtgaag atggatccat
     4501 acaaggtctg tttgaattgt ccggtatccc ttttgtaggc tgcgatattc aaagctcagc
     4561 aatttgtatg gacaaatcgt tgacatacat cgttgcgaaa aatgctggga tagctactcc
     4621 cgccttttgg gttattaata aagatgatag gccggtggca gctacgttta cctatcctgt
     4681 ttttgttaag ccggcgcgtt caggctcatc cttcggtgtg aaaaaagtca atagcgcgga
     4741 cgaattggac tacgcaattg aatcggcaag acaatatgac agcaaaatct taattgagca
     4801 ggctgtttcg ggctgtgagg tcggttgtgc ggtattggga aacagtgccg cgttagttgt
     4861 tggcgaggtg gaccaaatca ggctgcagta cggaatcttt cgtattcatc aggaagtcga
     4921 gccggaaaaa ggctctgaaa acgcagttat aaccgttccc gcagaccttt cagcagagga
     4981 gcgaggacgg atacaggaaa cggcaaaaaa aatatataaa gcgctcggct gtagaggtct
     5041 agcccgtgtg gatatgtttt tacaagataa cggccgcatt gtactgaacg aagtcaatac
     5101 tctgcccggt ttcacgtcat acagtcgtta tccccgtatg atggccgctg caggtattgc
     5161 acttcccgaa ctgattgacc gcttgatcgt attagcgtta aaggggtgat aagcatggaa
     5221 ataggattta cttttttaga tgaaatagta cacggtgttc gttgggacgc taaatatgcc
     5281 acttgggata atttcaccgg aaaaccggtt gacggttatg aagtaaatcg cattgtaggg
     5341 acatacgagt tggctgaatc gcttttgaag gcaaaagaac tggctgctac ccaagggtac
     5401 ggattgcttc tatgggacgg ttaccgtcct aagcgtgctg taaactgttt tatgcaatgg
     5461 gctgcacagc cggaaaataa cctgacaaag gaaagttatt atcccaatat tgaccgaact
     5521 gagatgattt caaaaggata cgtggcttca aaatcaagcc atagccgcgg cggttctgtt
     5581 gcaaagtttt aaataaagaa taaaatccct
//
LOCUS       DAZULO010000274         4705 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.guided.142, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000274 DAZULO010000000
VERSION     DAZULO010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.guided.142"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1336
                     /locus_tag="ACG8OA_003050"
     CDS             <1..1336
                     /locus_tag="ACG8OA_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFS4977755.1"
                     /translation="WDRRNLPLPDDKVNPTQYEYTINFKGFRNTYFKQLVKRYCKLRL
                     NMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSELNMMGIKPSTIT
                     GRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEFVLEQLNSHLDKL
                     PEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKMKKEHIVPISKEV
                     ALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQNIVDKSGEIYRF
                     HAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETLKNEFTKFQEKLV
                     TNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGCPHANACLDCTHF
                     CTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQIIGSLTG"
     gene            1343..1720
                     /locus_tag="ACG8OA_003051"
     CDS             1343..1720
                     /locus_tag="ACG8OA_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HFS4977756.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            1874..2656
                     /locus_tag="ACG8OA_003052"
     CDS             1874..2656
                     /locus_tag="ACG8OA_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HFS4977757.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(2782..3513)
                     /gene="erm(A)"
                     /locus_tag="ACG8OA_003053"
     CDS             complement(2782..3513)
                     /gene="erm(A)"
                     /locus_tag="ACG8OA_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HFS4977758.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(3571..3630)
                     /locus_tag="ACG8OA_003054"
     CDS             complement(3571..3630)
                     /locus_tag="ACG8OA_003054"
                     /inference="COORDINATES: protein motif:HMM:NF019858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HFS4977759.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            4022..4684
                     /locus_tag="ACG8OA_003055"
     CDS             4022..4684
                     /locus_tag="ACG8OA_003055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4977760.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
BASE COUNT         1656 a          682 c          895 g         1472 t
ORIGIN      
        1 ttgggataga cgtaacttac ctttgccaga tgataaggtt aacccaacac aatatgaata
       61 tacaattaac tttaaagggt ttcggaatac atattttaaa caacttgtaa aaagatattg
      121 taagttgaga ttgaacatgg atagcttttc ctatgtaagt gatattgccc aaagacttaa
      181 agagttcttt aattttctgg acatgaaatt taaacaagtt cagagagtac accaattaac
      241 gagagtggaa attgaagcat atttaagtga actaaacatg atgggaataa aacctagtac
      301 aataactggg aggatctcta tattggaagg actatttagt acccttctta ggctagaatg
      361 ggatgatgtt ccttccaaaa tattaattta ttctgaggac tatccgaaaa taccaagagc
      421 aaaaccacgc tttatagatg aattcgtcct agagcaattg aacagtcatc ttgataaatt
      481 acccgaatat atagctacga tgactatgat tgttcaagaa tgtggaatga ggataagtga
      541 attgtgcacc ttgaaaaaag gctgtctatt agaggacaaa gatggagatt tctttttaaa
      601 gtattatcaa tggaaaatga aaaaggagca tatagttcca atatctaaag aggtagcttt
      661 acttattaaa gttcgggaag ataaagtttc agaggaattt ccagatagtg aatacctctt
      721 tccaagaaaa gatggatcgc cattaaaaca agaaacattt agaggtgagt taaataaatt
      781 agcttatgag caaaatatag tggataaatc aggtgagatt tatagattcc atgcccatgc
      841 ctttcgccat acagtaggaa caagaatgat taacaacggg atgccccagc atattgtgca
      901 gaaatttttg gggcatgaaa gcccagaaat gacaagcaga tacgctcata tctttgatga
      961 aactctaaaa aatgaattta ctaaatttca ggaaaaactg gttaccaata atggagatgt
     1021 gcttgatcta gatgaagata atgaagtcga tgatgtagag cttcaatggt tcaagaaaaa
     1081 tataaatgca caagtgcttc caaatggtta ttgtagattg ccagtagtag caggtggttg
     1141 tccacatgcg aatgcatgct tagattgcac tcacttctgt accagtaagc aattcttacc
     1201 acagcacgaa gaacagttag agcgtacaga agagttatta gccatagcta aggataaaca
     1261 atggcaaaga caagtagaga ctaatagccg tgttaaagag cgtttagaac aaatcattgg
     1321 aagtttgacg gggtaattat caatggataa acaagttaga aatacaacag aaattgtacg
     1381 tttggcgaag cagaaatcaa aaaagacaag ggaaaaagta gacaaagcga tttctaaatt
     1441 ttcgattgaa ggtaaagtta ttaattttaa ttcaatagca aaggaagcta atgtttctaa
     1501 atcatggctt tataaggaac acgatattag gcaaagaatc gaatcccttc gtgagcgtca
     1561 aataacagca aatgtagtct caaaacccaa gaaaagttct cgttcggagg aaatccttat
     1621 taaaacctta aaaagaagag taatggaatt agaaaaagaa aataaaaaat tacagaacca
     1681 aattcaaaaa ttatatggag atctgtataa taaagaataa ttattaatct gtagacaaat
     1741 tgtgaaagga tgtacttaaa cgctaacggt cagctttatt gaacagtcat ttaggtatat
     1801 gtccaatcta gggtaagtaa attgagtatc aatataaact ttatatgaac ataatcagtt
     1861 acgaggtgaa atcatgagca atttgattaa cggaaaaata ccaaatcaag cgattcaaac
     1921 attaaaaatc gtaaaagatt tatttggaag ttcaatagtt ggagtatatc tatttggttc
     1981 agcagtaaat ggtggtttac gcattaacag cgatgtagat gttctagtcg tcgtgaatca
     2041 tagtttacct caattaactc gaaaaaaact aacagaaaga ctaatgacta tatcaggaaa
     2101 gattggaaat acggattctg ttagaccact tgaagttacg gttataaata ggagtgaagt
     2161 tgtcccttgg caatatcctc caaaaagaga atttatatac ggtgagtggc tcaggggtga
     2221 atttgagaat ggacaaattc aggaaccaag ctatgatcct gatttggcta ttgttttagc
     2281 acaagcaaga aagaatagta tttctctatt tggtcctgat tcttcaagta tacttgtctc
     2341 cgtacctttg acagatattc gaagagcaat taaggattct ttgccagaac taattgaggg
     2401 gataaaaggt gatgagcgta atgtaatttt aaccctagct cgaatgtggc aaacagtgac
     2461 tactggtgaa attacctcga aagatgtcgc tgcagaatgg gctatacctc ttttacctaa
     2521 agagcatgta actttactgg atatagctag aaaaggctat cggggagagt gtgatgataa
     2581 gtgggaagga ctatattcaa aggtgaaagc actcgttaag tatatgaaaa attctataga
     2641 aacttctctc aattaggcta attttattgc aataacaggt gcttactttt aaaactactg
     2701 atttattgat aaatattgaa caatttttgg gaagaataaa gcgtcctctt gtgaaattag
     2761 agaacgcttt attactttaa tttagtgaaa caatttgtaa ctattgaaaa tagaaagaaa
     2821 ttgttccttc gatagtttat taatattagt gacatttgca tgcttcaaag cctgtcggaa
     2881 ttggttttta gtgaaaagaa cacgatattc acggtttacc cacttataaa caaaagatcg
     2941 atactttttg tagtccttct ttgaaatcaa tggttgatgt cgttcaagaa caatcaatac
     3001 agagtctaca cttggcttag gatgaaaata tagtggtggt acttttttga gcatttttat
     3061 atccatctcc accattaata gtaaacccaa agctcgttgc agattttgca atcttttcgc
     3121 aaatcccttc tcaacgataa gatagctata tttagcctga ctttcaaagg taattctttt
     3181 gacaatatcc gtactgatgt tataaggaat attaccatat atcttatagt ttatatgttt
     3241 tgggaaggaa aattttagaa tatccgtttg aatcactttt atattctcag aggggtttac
     3301 cgcttcttta gtcacttgac ataagcctcc atcaatttct atagcagtaa ctgatcgact
     3361 cattttgact agctctttgg taaaatgtcc ttttcctgat ccgatttcta ttacgttgtc
     3421 ttgtttactg atattcgtgt gattcaatat ttcttttaca tgctttttag aagtaataaa
     3481 attttgcgtg tctttagggt ttttctggtt cattataacc ttctccttac tggttataat
     3541 gaactgacat taagcgttca ttataaccaa ttaattttga tttggttgat aacgaacttt
     3601 attaataaca aatatagaaa aagtacccat acttttatct cctttccatt tttttcatta
     3661 tgaatgagat aaagtaattt ctactactgc gatactggtg cacataattg aaacctcctt
     3721 atgtaactga atatgattgt atcatctttt cattttagaa taaaccttta tatgtaattt
     3781 tgcacttgac acttcagatg gacgttttct tcaaaataat atgttttgaa acttgataag
     3841 agaagccact gtctttacta aaaagtaaag gctgcgataa aggacagtga ctttaataaa
     3901 gttgcactgt tgtattgtct ttggataaaa ttgtctaaag ggaagtttta tccccaacga
     3961 aaaaacatct aaagtggtaa gtaatgaaag aatgtcgata aagtaggaat gggggatctt
     4021 tttgaataca atagaatcac agtttgataa ggttgcagaa gattacgatt tcgtgaatga
     4081 gcttttgaat gattattcat tctttgtgtc taatatgtct ccaaagaaag gcagagcatt
     4141 agatatcgga tgtggctcgg gtttgttagt ggagaaatta gcaagttatt atgatgaagt
     4201 ggtagggatt gatatttcta atcaaatgct cgatcttgcc aaatctaaac gtcaactaac
     4261 aaatacggtc tatctgaata tgaatgcaga acaacttaat tttaatgaga agtttgattt
     4321 tattgtaagc cgaacaacct ttcatcattt ggatgatata gccagcgtaa tacagcaaat
     4381 gaaggaactg ttgaatgagg aaggaagaat agttattctt gataatgtat ctgaagtcga
     4441 gacaccgcct acctatgttt ataaattagg ggcaatccaa gagtttttac cgcattgttt
     4501 caaatttgga ataaaaaatg cgattagaat ttataatcac aacacatcta aatcatggct
     4561 tgagcattta gcttcagata agtacctatc tgaacaaaat tattacgatt tatatgaaaa
     4621 gttgttgcct ggatgccaat tccataaaat gggttgggca atgggggttg tctggacaaa
     4681 ataacttaaa gctgtggctt tgaaa
//
LOCUS       DAZULO010000275         3305 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.guided.171, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000275 DAZULO010000000
VERSION     DAZULO010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.guided.171"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1146)
                     /locus_tag="ACG8OA_003056"
     CDS             complement(<1..1146)
                     /locus_tag="ACG8OA_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977761.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVIL"
     gene            1239..1588
                     /locus_tag="ACG8OA_003057"
                     /pseudo
     CDS             1239..1588
                     /locus_tag="ACG8OA_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1860..2150)
                     /locus_tag="ACG8OA_003058"
     CDS             complement(1860..2150)
                     /locus_tag="ACG8OA_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4977762.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(2358..3050)
                     /locus_tag="ACG8OA_003059"
     CDS             complement(2358..3050)
                     /locus_tag="ACG8OA_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS4977763.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1057 a          589 c          456 g         1203 t
ORIGIN      
        1 caaaataact aaaaaaatag aagttttctt attgtcaaaa cttaaataaa ttccaacaat
       61 taaacatagt aataatgcca agactactaa agtagctatc caagtacctg gataaaaaag
      121 tgacagtttc gttcctaact ctgtaaagag attattcagg ttactactac tccaaatcat
      181 tgccctttta tttccattga cgtaagtaaa gctttgaccc attgtattac caaaataaat
      241 aatcaggttt aagaaaaacg gtaatgcagt caatatacgc acataatagt tttgacattt
      301 cctatatgtt agaaggaaaa ttaagaaaaa tagcaataaa aataagatat tcatgtctaa
      361 aaaaaacggc tttccaaatg atgaaatacc taaatctaac ttattaagaa agttgaaatt
      421 tttatattca ggaaaccatt tattaatttc atgtgcagca cgtaatgcat tccctggagt
      481 tgtcaaagag aaaattaatt ctgctaagct tattattgaa aatactgata acttataatt
      541 atatcgtcca ttgacaatca aatataaact aactatactt gttagaacaa aaaaacaggc
      601 gttgacttgt tcttgattag ttaaaaatat caataaagga atacttaccc aatatatttt
      661 tctatttatt tcttctcctc ttaagagttg aaaaaacgga taaaaagtta gtaaactaaa
      721 tgctactggc cactgatagt tcaaggtggt tgctctccaa ccggtttccc ccattactgt
      781 caatggtatt aaacagtata tagagcaaac caataataaa tttttaacat ttattttctc
      841 aaaaacatac ttgcaaagta atactacaat tcctaacata atcgtagaat ttaataattt
      901 ccatagcata aagtgtttag aaaagaacat taagaatagt tctataataa ttcttgaact
      961 ccatgtctta taatgttcta tcgaagcacc caaatagcca agttcatcta atctacttgc
     1021 ataaactacg tcatccgctc tcatagtcgg catatacata ttaaagagta aaaagatacc
     1081 ccaaatccct attagtaaca agatcagttc tttgttattc gttatttttc tattatgttt
     1141 gttcattttt atactccttc ttatatttcc taatagagta taacatagct ttaagcatta
     1201 cttaaagata acttaaaagt tatcttttaa atacttaatt gttaaatacc ttatttgatg
     1261 caaaagaaat ctacgatagt caacaaaaaa attttcatta tatccagaaa aatttttctt
     1321 agttaaggac ctatggattg cagtaatgca gaattcgtcg aataaactgc caaagacata
     1381 caatcatatt gcttttaaaa ttgacgaaca agatatagat tcgtttgtct caaaaatcca
     1441 aatgttaggt ttaactgtcg aaccaggacg ttctcgtgtg aaaggcgagg cttcttcaat
     1501 ttacttttac gactatgata atcacctctt cgagttacat accggtactc tccaggaacg
     1561 tttaaaatcc tacaattcca ctacctgaga ctagtaatga ctagtctctt tttatggcct
     1621 ctgatccccc cattgagcga atactaatag ggcttcgaat tgatctgttc aatctttaag
     1681 caatatccct caaatgagtt tggaaagttt agtgattcat cacaaatact taaatacatg
     1741 tagttatata tagattgagg ttcttgtgag cgtatgctcg tgtgggttca aatcccatcg
     1801 tctgcatcca tctttccaaa acaagcggct aggacatttt tgtctcagcc gcttgttttc
     1861 tagtttattc tttcatcttg tcggaccttt tttcttgggt atagactttt ctcatattct
     1921 accacaattt tgggtttccc taacacatga taggatctca caacaaattg ctttctatta
     1981 ttataataac ctgtaacgac taacttcatc ccttcgtcta cttcagctag gaagttcaaa
     2041 ctatgggaag caatcaaaca attgacccca tttaaactga aacggatcaa cggtctggca
     2101 ctaacttgca aagtacggat cttatctact gttccatatt ttttctccat aaccttcacc
     2161 tcaataccat tatacgaaca tacattccct ttgtaaagaa gaaactctgt tttatctttt
     2221 tgactttttg tctattacct ttcttaaaat catattggca cttcctgcga agatgaagga
     2281 catgtactaa aaaaaagagg cagcagtata gattactgtt gcttctttac taatttaggt
     2341 ttacttgtta agaaagatta tttacgcaaa tgttcttcaa caatttgctg agatttatca
     2401 gctgtatctt ccacttcatc ttttgctttt tctaactttt gaatatattg tttcaagctt
     2461 gagttttcag cagttgcttc gtctaatttc ttttgtgtat ctgttaactg agtttctaca
     2521 tttgtttttt cttgtgtcag tgttgcgatt tgttgattca aaccatttac agtattttgt
     2581 aattctgaaa gttttgcttg tgcttcttgt agtcttgctg tccaatctgc attcagatga
     2641 ttgatttcat catttttttg gttgatctca tctagtttga cattgatttc attttgtttg
     2701 ctttgtagtt gtgcatcctt gctgactaat tgactttcta agttttgttt ttctgctgcc
     2761 gattgatttt tttggttttc taattcattt acttttgttt ggtattcttg gacatctgcc
     2821 tttgaatctt gcaatgtctg ttctttttgt gctagtcgag cggtaagatc tgtaagcgct
     2881 tgctctttat ctgtcaatgt atgatcaagc tgtgtcagat tctcattgat tgtatcaata
     2941 tctgtctctc caccccacaa aataatttca tttgcgtacc gcgtgcctgt gtagccaatt
     3001 agacatagaa atgcaacgac aatcccacca gtaataaatt tctttttcat ttgcatagct
     3061 ccttaatcca atacttttac atgctaaaag taagtgatct caaacacaat gtcaagcgtt
     3121 tttaaattac atgtgtaatt ttaatataaa tactatcaaa tactcattta atcctccttt
     3181 tttatctatt taaaaaatct tttttataga gaaaaatgca ggttaatact ttcttaaata
     3241 aatatttatg gtgaacaaaa acaaaaaagc aagttttatt aaacgtaacc cttttcattt
     3301 catag
//
LOCUS       DAZULO010000276         1504 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.guided.206, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000276 DAZULO010000000
VERSION     DAZULO010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.guided.206"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            262..345
                     /locus_tag="ACG8OA_003060"
                     /pseudo
     CDS             262..345
                     /locus_tag="ACG8OA_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            394..477
                     /locus_tag="ACG8OA_003061"
     CDS             394..477
                     /locus_tag="ACG8OA_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFS4977764.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="ACG8OA_003062"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="ACG8OA_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFS4977765.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DAZULO010000277         1349 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.guided.214, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000277 DAZULO010000000
VERSION     DAZULO010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.guided.214"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..297
                     /locus_tag="ACG8OA_003063"
                     /pseudo
     CDS             46..297
                     /locus_tag="ACG8OA_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="ACG8OA_003064"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="ACG8OA_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFS4977766.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
LOCUS       DAZULO010000278         1209 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.guided.221, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000278 DAZULO010000000
VERSION     DAZULO010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.guided.221"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1141
                     /locus_tag="ACG8OA_003065"
                     /note="aph(2'')-Ia"
     CDS             <1..1141
                     /locus_tag="ACG8OA_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HFS4977767.1"
                     /translation="GFYSLFKTLQQNRTRYIKLIFEFLKKERNANAVILDPHKNNPRA
                     IRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKV
                     DSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNI
                     EYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDIS
                     ECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFS
                     CNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDI
                     EKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          521 a           91 c          196 g          401 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccgt acaagatata ttaaattgat
       61 ttttgaattt ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa
      121 taatccaaga gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc
      181 agaacatgaa ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga
      241 tgataatgcc acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt
      301 caaagtagat agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa
      361 taatgaatac atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga
      421 aaaagcaata tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat
      481 tgaatattcg tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac
      541 ttttttaaca ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg
      601 agatattgcc agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg
      661 tactattgat aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta
      721 taatgattta actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc
      781 aacaacagtt tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct
      841 attgttagat ggcaataata gattaactgg aataattgat tttggagatt ctggaattat
      901 agatgaatat tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa
      961 ttttggagaa gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata
     1021 tcaagatata gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat
     1081 taaacaggaa tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg
     1141 attatataat atatgaaaag ctattataaa agacattagt attaaatagt ttaaaaaaat
     1201 gaaaaataa
//
LOCUS       DAZULO010000279         1037 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5148
            SAMEA115707527-rid22576593.guided.239, whole genome shotgun
            sequence.
ACCESSION   DAZULO010000279 DAZULO010000000
VERSION     DAZULO010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707527
            Sequence Read Archive: ERR13197256
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288058.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 32 of 93
            Pseudo Genes (incomplete)         :: 52 of 93
            Pseudo Genes (internal stop)      :: 26 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707527-rid22576593.guided.239"
                     /isolate="VRE5148"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            322..870
                     /gene="aac(6')"
                     /locus_tag="ACG8OA_003066"
     CDS             322..870
                     /gene="aac(6')"
                     /locus_tag="ACG8OA_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS4977768.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          367 a          149 c          210 g          311 t
ORIGIN      
        1 cttttttgta attatctaaa aattctaaaa attaagatta ttatatgaaa acaggtttta
       61 tttgtcaatt cataattggt tttttttaaa gcaaaagata atctatatca tgatgaacaa
      121 acagagtttt ttcctccaat tgatggcttt cctgatcctt cctattttga ataaagtgtt
      181 tctgtataat ttttttattt tataagtaag ataaataatg aaagaataga gatactgatt
      241 ggtagctaaa gatttttgag cgagaacatt tcgttttttc aaaaggactg ttagaataag
      301 caagaacaag ggggaagacg tatgataatc agtgaatttg accgtaataa tccagtattg
      361 aaagatcagc tttctgattt actgagactg acttggccgg aagaatatgg agacagctcg
      421 gcagaagaag tagaagaaat gatgaatcca gaacgaatcg cggtagcagc ggtagaccaa
      481 gatgagttag taggatttat tggtgcaatc cctcaatacg gtatcacagg ttgggaattg
      541 catccattag ttgtagaaag ctcccgacga aagaaccaaa taggtactcg attagtcaat
      601 tacttagaaa aagaagtagc ttccagagga ggaatcacga tttatttagg tacggatgat
      661 ttagaccatg gaacaacgtt aagtcaaacg gacctgtatg agcatacatt tgataaagtg
      721 gcttccatcc agaaccttcg tgaacatccg tatgaattct atgaaaaatt aggttataaa
      781 atcgtaggtg tcttaccaaa tgcaaatggc tgggacaaac cggatatttg gatggcaaaa
      841 acgattattc ctcgaccaaa ttctcaataa taaaaagagg ttgtgacaga agtaatcagc
      901 ttcaagaaat aagaaggaat ttacgaaaat tacttcttga atttttgtga aattccaact
      961 tatttccgaa gaagctactt ttggaacacc gtgtaccaga ggttgtgaaa aagtcgctta
     1021 gtttcgagta gtaagaa
//
